text:
Zheng Xicheng , 71, has just published a collection of drawings of Beijing courtyard houses, in an effort to raise public awareness of the need to protect these relics.
Why did you publish this book? How did you come up with the idea?Sunday, 14 June, 2009, 12:00am
We have been hearing horror stories about climate change and the extinction of animal species for years, but Home - a soon to be released documentary - prefers to dwell on the beauty of what is left.3 Jun 2009 - 12:00am
by Marilynne Robinson
Virago, HK$10410 May 2009 - 12:00am
The latest record from Italian-Japanese singer-songwriter Angela Aki is everything its title Answer suggests. She tells us her views and answers to the questions of life, love and music.
Inspired by the legendary Sarah McLachlan, Aki broke into the mainstream Japanese pop music scene with her refreshingly gentle and wonderfully soothing debut Home in 2006.26 Apr 2009 - 12:00am
There's a quiet revolution on the domestic front in Island South - and it's just one of the ways in which the owner of Belgravia apartments hopes to clean up. Executives at Kerry Properties say the refurbished apartments in South Bay Road boast the first centralised vacuum-cleaning system of its type to be used in a residential building in Hong Kong.13 Mar 2009 - 12:00am
There's a new stack of serviced apartments in Central. The Yin was opened on Thursday with a cosy cocktail gathering at its Wellington Street premises, in close proximity to the Graham Street wet market. Offering all the modern amenities any Blackberry-carrying executive needs in a slick rooming package, architect Philip Liao Yi-kang described its look as 'industrial chic'.23 Feb 2009 - 12:00am
Dennis Yip Chor-hong had a brilliant day at the office, not only landing a 330-1 double with Sunrise and Local Power but seeing a return to form of two of last season's more promising horses as well. Sunrise, 12-1 winner of the fifth event, was the first leg of Yip's double and the second of a two-timer for Brett Prebble.9 Feb 2009 - 12:00am
While I don't like the cold weather, I love the food we tend to eat at this time of year. The following two main courses are easy to cook and the results are hearty and satisfying. The dessert is warming because of the ginger.
Honeycomb tripe with parmesan cheese (pictured)1 Feb 2009 - 12:00am
The multimillion-dollar Christmas hampers once exchanged by the rich and famous appear to have fallen victim to the financial crisis, but shops and hotels are hoping a range of less pricey gift packs will persuade people to spend.15 Nov 2008 - 12:00am
Kitchens are capable of hiding secrets. Behind those shiny cupboard doors, in hard-to-reach corners and the darkest depths beyond the sink, can exist all manner of strange and unusual things. And they can be just what we'd all love to have in our homes.26 Sep 2008 - 12:00am
Any flat flipper will know that handsome kitchens add value to homes. I don't intend to sell my place in the near future but as a typical Hongkonger I can't help but consider my return on investment. That was before I checked out the options.29 Aug 2008 - 12:00am
The State Council, the mainland's cabinet, plans to introduce new laws this year to create more affordable housing for low-income families.
But analysts say the legislation is unlikely to be the comprehensive solution the mainland needs to bring down housing prices.18 Mar 2008 - 12:00am
Born to a Japanese father and an Italian-American mother, Angela Aki made her name among the list of popular J-pop singers with her debut Home, which sold over 500,000 within its first year of release in 2006.
Now with her second album, Today, Aki shows she still has what it takes to produce another success.
Today features 13 piano pop tracks written by Aki.17 Feb 2008 - 12:00am
What's going on around the globe
An unmade bed might not seem the most obvious inspiration for an artist. But to Brooklyn-based Chris Doyle, staying at numerous hotels across the US got him thinking about the status of the bed and provided the impetus behind a solo exhibition in Los Angeles.3 Feb 2008 - 12:00am
Question 1
What did Mr Jones leave in the hotel?
Mr Jones has just got home after a business trip. But he left something behind at the hotel and he's phoning there now to ask if they have found it. Listen to the conversation and answer the questions.
1. What did Mr Jones leave in his hotel room?25 Jan 2008 - 12:00am


text:
New Laundry Room!
It is small but it is done and I love it, especially after having to go out to the garage to do laundry before!
Washer and Dryer: Samsung
Cabinet/wall/trim paint: BM Richmond Grey
Counter: Desert Limestone Quartz
V-Groove Backsplash
Moen Faucet
Tile: 17x17 Rocks Beige with Oyster Grey grout
Hardware: Emtek 86092 - SP
Laundry Rack by Ballard Design
Congratulations!! Your new room is awesome, and it does not look small! My laundry room is for sure smaller, and while it has a door leading outside..there is no window! I love your window, and the previous member stated; it lets in great natural light. Very classic, tasteful and timeless choice for materials and colours! Excellent!
Wow, you did a fabulous job on this! Beautiful Laundry room...I only wish my laundry room were this small....in that I mean, mine is smaller. :)
I'm still in renovation and loving your Ballard Laundry Rack. Are they actually built into the wall? I can still do this I think. Thanks for sharing your photo.
Beautiful! Love the beadboard backsplash and the laundry rack. Did you ever consider stacking the units rather than opting for pedastals? Just starting our reno and I'm still deciding. I have space for either arrangement,but will likely opt to stack.
Thanks for the compliments!
The laundry rack is from Ballard. I didn't like the white with antique white color (I don't know why they didn't make it all white). My cabinet maker sprayed it the color of my cabinets so it blends in. It is just hung on the wall like normal.
We bought the washer/dryer before the reno and they were out in the garage and we got the pedestals then. I did consider stacking though so I could have more counter space. That would definitely have been a good option.
My laundry room definitely makes doing laundry feel less like a chore!
peteyjd: The pull down rack over the washer is by a company called Hafele. I believe it is a German company. My cabinet maker ordered it for me, but I think you may be able to find it online. It solved the problem of where to put hanging clothes. I didn't want a bar over the sink at the window and since I have pedestals under the W/D it needed to be high. It pulls down so I can reach it. It isn't very pretty but it is functional!
Thanks hoyamom! I throw the clothes over the rungs of the rack like you would a regular drying rack. I was concerned about how well the clothes would dry as they overlap a little but it works great. I love my rack. Ballard does sell one that has something at the bottom that flips out to hang things on hangers if you want both options.

subset: commoncrawl


text:
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Narrow your search
Which has the most apps? Which has the coolest features? Which one is the best? The most popular streamers all have their merits, so we'll help you decide which box is right for you.
Sling TV is a new $20-per-month live TV package with 16 channels, including ESPN, AMC, TNT, CNN, History, HDTV and the Disney Channel. How does it work, how can you get it -- and will it let you quit cable?
So-called 4K video could arrive later and cost more because of a surprise royalty demand for a certain video compression technology.
It's unlikely to dethrone major names like Chrome and IE, but CEO Jon von Tetzchner hopes Vivaldi will attract power users who want a full-featured PC browser.
The search giant is reportedly adapting its popular mobile game Ingress for television. But don't expect Google to develop more TV shows.
The latest version of the Opera browser for desktops comes with a new tool that lets you share multiple bookmarks at once.
That's an awfully inexpensive way to give your TV an awfully good audio upgrade.
The Opera Coast 4.0 update includes a new Turbo mode that uses Opera servers to compress images and videos for faster page loads.
The mobile browser now has the third largest user base of any app, behind WhatsApp and Facebook.
European carrier giant Telefonica, parent company of the UK's O2, has embraced WebRTC technology so its customers just need a browser to make calls. Too bad about Apple's Safari, though.


text:
See our store section for gifts by different stores
See our top gift ideas section for most popular items.
Create your own personal gift registry. Register for any item, from any store, for any occasion. Find the gift registries of your family and friends.
Ever find the perfect gift idea for someone, but their birthday was months away? We make it easy to remember your gift ideas.
Provides timely email reminders for a birthday, anniversary, and other special occasions.
Get advice from friends and family on which gifts to buy.
Sterling Silver
A Mother's Heart Birthstone Charm Angel Figurine
Personalized 7-Diamond Love Ring
Our Family Of Love Personalized Birthstone Ring
Personalized Linked By Love Necklace
Forever in a Mother's Heart: Personalized Birthstone Necklace
Personalized Birthstone Family Tree Necklace
A Mother's Faith and Family Pendant
Two Hearts Become One Personalized Gemstone and Diamond Ring
Love Blooms Forever Personalized Sterling Silver Pendant
Granddaughter Love Bug Birthstone Personalized Music Box
Mother Holds Her Child's Heart Personalized Necklace
My Daughter I Love You Always Personalized Birthstone Music Box
Mother's Precious Gems Birthstone Necklace
Granddaughter Fills My Heart Engraved Birthstone Music Box
Swarovski Crystal Birthstone Mother's Infinite Joy Pendant
My Heart Belongs to My Grandkids Personalized Necklace
Love Holds Our Family Together Personalized Birthstone Ring
Sky Gazer Created Star Sapphire and White Topaz Ring
Personalized Bracelet with Family Birthstones and Names
A Mother's Embrace Personalized Birthstone Ring
Blessing of the Claddagh Emerald & Diamond Hidden Message Ring
Dazzling Delight Multi-Gemstone Necklace
Pride of New England Patriots Blue Sapphire and Ruby Ring
Serenity Diamond and Sapphire Eternity Ring


text:
Nobilo Sauvignon Blanc 750ml$15.54
Nobilo Sauvignon Blanc
Nobilo - Marlborough - New Zealand
Sauvignon Blanc grapes originated in France, where they are still widely grown today and treated to a wide range of processing practices from aging in oak barrels, blending with other varietals, and undergoing malolactic fermentation to encourage a more mellow, buttery finish. These green skinned white wine grapes are highly versatile, and are now grown in several countries around the world which have the correct climatic conditions for getting the best results from them. Generally, Sauvignon Blanc varietal grapes prefer a cooler climate, as too much heat dulls the flavor present in the fruit. As such, they are generally grown in valleys and on coastal areas, where they can benefit from cooling breezes before being typically harvested early in the summer. The grapes themselves produce wines which are often very dry and crisp, yet full of a wide range of flavors including grasses, tropical fruits and citrus notes.
When it comes to New Zealand wines, the Marlborough region is the most highly regarded and by far the largest in the country. The region itself has been closely associated with high quality white wines for over forty years now, and consistently produces some of the finest Sauvignon Blanc wines in the world. However, in recent years it has begun to expand its repertoire, and wineries have been busily raising Pinot Noir and Chardonnay vines in Marlborough, amongst certain others. The excellent soil and ideal climate the region benefits from ensures the fruit there ripens slowly, achieving maximum flavor and expression of terroir. Furthermore, Marlborough's situation on the north easternmost tip of New Zealand's South Island ensures there are plenty of cooling oceanic breezes crossing the vineyards, making this one of the most perfect locations in the world for quality viticulture.
New Zealand is widely regarded as one of the most beautiful and ecologically diverse countries in the world, and the wines which are produced there are a fine representation of this diversity and overall quality. With beautifully long and warm summers, a brisk oceanic climate and mile upon mile of gently sloping mountainsides, the vineyards which have sprung up there over the past century have brought about truly fantastic results, with wine now contributing a considerable amount to the economy of the country. In recent years, New Zealand wines have won some of the most prestigious awards available in the wine world, with most of the lauded wineries coming from the Auckland region, where there has been considerable effort to replicate the finest wines of the Bordeaux region of France. The Bordeaux grape varietals (Cabernet Sauvignon, Merlot, Cabernet Franc, Petit Verdot and Malbec) all flourish in the mineral rich soil of the region, and the results have been outstanding, marking New Zealand a strong contender for finest New World wine country.


text:
Page 1
Effects of Interferons a/b on the Proliferation
of Human Micro- and Macrovascular Endothelial Cells
Joris Erdmann,1,2Giovanni Vitale,2–4Peter M. van Koetsveld,2Ed Croze,5Diane M. Sprij-Mooij,2
Leo J. Hofland,2and Casper H.J. van Eijck1
Synthetic interferons (IFNs) are used in the treatment of several types of cancer. In addition to an antitumor effect,
IFNs show antiangiogenic activity. The aim of this study was to investigate the effects of IFN-a and IFN-b
on human micro- and macrovascular endothelial cells in vitro [human micro vascular lung endothelial cells
(HMVEC-L) and human umbilical cord endothelial cells (HUVEC)]. By immunohistochemical staining and
quantitative reverse transcriptase (RT)-polymerase chain reaction, we studied expression of type I IFN receptors.
We evaluated the effects of IFN-a and IFN-b on the proliferation (DNA content), apoptosis (DNA fragmentation by
enzyme-linked immunosorbent assay), and cell cycle distribution (flow-cytometric analysis) of endothelial cells.
HUVEC and HMVEC-L cells show comparable expression level of the distinct IFN receptor subtypes. Proliferation
of HMVEC-L and HUVEC was inhibited by IFN-b (the half maximal inhibitory concentration [IC50]¼60 and
90IU/mL, respectively), but not by IFN-a at a dose up to 1,000IU/mL. An interesting and unexpected observation
was an inhibition of apoptosis by IFN-b. After 72h of treatment with IFN-b. Cell cycle inhibition occurs in late S-
phase in both cell lines. In conclusion, only IFN-b, not IFN-a (10–1,000IU/mL), has an inhibitory activity on
endothelial cell proliferation. Surprisingly, apoptosis was decreased by IFN treatment, whereas inhibition of
proliferation is caused by cell cycle arrest in late S-phase.
1957) as cytokines released by virus-infected cells inducing
endonucleases and inhibitors of protein synthesis in neigh-
bouring cells creating an antiviral state (Samuel 2001). Syn-
thetic IFNs are currently used in the treatment of several types
of cancer, multiple sclerosis, and viral infections such as hep-
atitis B/C and severe acute respiratory syndrome. IFNs are
highly species specific and consist of 3 families of glycopro-
teins: type 1–3 IFNs. The type 1 family includes the IFN-a
(leukocyte IFN), IFN-b (fibroblast IFN), IFN-o, and IFN-t
subtypes. Type 2 (IFN-g) and the recently discovered type 3
IFNs (IFN-l) (Kotenko and others 2003) will not be further
discussed in this article. Thirteen forms of IFN-a and only one
of IFN-b are known to exist (Roberts and others 1998). The
type 1–3 IFNs act through separate membrane receptors that
have distinct properties and induce different responses. The
type 1 receptor consists of 2 subunits, AR1 and AR2. AR2 is
known to have 3 splice variants: long, short, and soluble forms.
nterferons (IFNs) were first discovered in 1956 by
Alick Isaacs and Jean Lindemann (Isaacs and Lindenmann
Only one form of AR1 has been identified (Novick and others
1994). Binding of IFNs to their receptor causes hetero-
dimerization of the 2 receptor subunits, AR1 and AR2c, the
long form splice variant (Croze and others 1996). One of the 2
membrane-bound variants of AR2, the short form AR2b, is
able to bind IFNs but does not couple to signal transduction
because it lacks the signal transducing tail of AR2c (Novick
and others 1994, 1995). This soluble AR2a may act as regula-
and others 2004). After heterodimerization, Tyk 2 and Jak 1
associate with the receptor complex and form a docking site
for STATS 1 and 2. These STATS then translocate to the nu-
cleus were they activate transcription of IFN-stimulated genes
(Caraglia and others 1997, 2005; Jonasch and Haluska 2001).
The list of IFN-stimulated genes is still increasing and currently
over 300 genes have been identified (de Veer and others 2001).
IFN-a and IFN-b induce different subsets of genes in fibrosar-
coma cells (Der and others 1998); however, no differences were
found in endothelial cells (da Silva and others 2002).
IFNs have known antitumor effects (Jonasch and Haluska
2001) and we recently demonstrated a more potent in vitro
1Department of Surgery, Erasmus MC, Rotterdam, The Netherlands.
2Department of Internal Medicine, Division Endocrinology, Erasmus MC, Rotterdam, The Netherlands.
3Chair of Endocrinology, Department of Medical Sciences, Faculty of Medicine, University of Milan, Milan, Italy.
4IRCCS, Instituto Auxologico Italiano, Milan, Italy.
5Department of Immunology, Berlex Bioscience, Inc., Richmond, California.
Volume 31, Number 5, 2011
ª Mary Ann Liebert, Inc.
DOI: 10.1089/jir.2009.0103
Page 2
antitumor activity of IFN-b compared to IFN-a in adrenal,
pancreatic, and neuroendocrine tumors (van Koetsveld and
others 2006; Vitale and others 2006, 2007).
In vivo studies show growth inhibition of several types of
cancer by IFNs. Inability to respond to endogenous IFN-a
and IFN-b in type 1 receptor-deficient mice accelerated de-
velopment, tumorigenity, and metastasis of cutaneous neo-
plasms (McCarty and others 2002). In hepatocellular
carcinoma grown in nude mice, IFN-a inhibits metastasis
and recurrence after curative resection by inhibition of an-
giogenesis mediated through downregulation of vascular
endothelial growth factor (Wang and others 2003). Gene-
therapy-delivered IFN-b is able to reduce invasiveness and
angiogenic potential of prostate and renal cell cancer in vivo
(Dong and others 1999; Nakanishi and others 2003).
It is clear that endothelial cells play a critical role in tumor
growth, since the size to which a tumor can grow is limited
to the diffussional capacity of the tissue for oxygen and nu-
trients. An angiogenic switch (Folkman 2002) is necessary to
expand beyond this point. Proliferation of endothelial cells is
thought to be an essential step in this process of angiogen-
esis; therefore, inhibition of proliferation may result in anti-
angiogenic effects (Carmeliet 2003; Tonini and others 2003;
Taraboletti and Giavazzi 2004). In vitro both stimulatory
(Cozzolino and others 1993; Mintzer and others 1998; Gomez
and Reich 2003) and inhibitory (Heyns and others 1985;
Hicks and others 1989; Ruszczak and others 1990; Yoshida
and others 1996; Hong and others 2000; Minischetti and
others 2000; da Silva and others 2002; Pammer and others
2006) effects of type 1 IFNs on endothelial proliferation have
been reported. Remarkably, IFN-b, potentially the most po-
tent subtype (van Koetsveld and others 2006; Vitale and
others 2006, 2007), has not been studied extensively. There-
fore, in the current study a comparison is made between the
anti-proliferative effects of IFN-a and IFN-b on micro- and
macro vascular endothelial cells in vitro.
Materials and Methods
Cell culture
Human umbilical cord endothelial cells (HUVEC) and
human microvascular lung endothelial cells (HMVEC-L)
were supplied by Clonetics-Cambrex Biosciencea ¨ (Walkers-
ville), and cultured in EBM-2MV?medium supplied by the
same company. All supplements (human epidermal growth
factor [hEGF], Hydrocortisone, vascular endothelial growth
factor, human fibroblast growth factor [hFGF-B], Long R3-
IGF-1, Ascorbic Acid, GA-1000 [antibiotics], and fetal bovine
serum 5%) were added to the medium. The cultures were
grown at 378C and 5% CO2. Standard culture instructions
supplied by Clonetics were applied. Cells were passaged by
Grand Island, Canada). Before plating, the cells were coun-
ted microscopically using a standard heamocytometer. Try-
pan Blue staining was used to assess cell viability, which
always exceeded 95%. HMVEC-L were used only from
passages 3–6 and HUVEC, passages 3–7.
[0.05%/0.53mM]; Gibco,
Drugs and reagents
Intron-A (IFN-a-2b), REBIF (Recombinant IFN-b 1a), and
Poly I:C were obtained from Schering-Plough Corporation
(Utrecht, The Netherlands), Serono (Rockland, MA), and
Sigma-Aldrich (Leiden, The Netherlands), respectively. All
compounds were stored at ?208C, and the stock solution
was constituted in distilled water according to the manu-
facturer's instructions.
Quantitative reverse transcriptase-polymerase
chain reaction
Expression of receptors (AR1, AR2 total, the short-form
AR2b, and the long-form AR2c) and housekeeping gene
was evaluated by quantitative reverse transcriptase (RT)-
polymerase chain reaction (PCR) in HUVEC and HMVEC-L
cells as published previously (Vitale and others 2006). In ad-
dition, IFN-b primer and probe sequences were purchased
from Biosource (Nivelles, Belgium): forward, 50-CAGCAATTT
TCAGTGTCAGAAGCT-30; reverse, 50-TTCATCCTGTCCTT
GAGGCAG-30; probe 50-FAM TGTGGCAATTGAATGGGAG
The detection of HPRT mRNA was used for normalization
of mRNA levels. Expression of AR2a mRNA, the soluble
form of AR2 subunit, was determined indirectly by sub-
tracting AR2b and AR2c from AR2 total. Several controls
were included in the RT-PCR experiments. To exclude con-
tamination of the PCR reaction mixtures, the reactions were
also performed in the absence of DNA template in parallel
with cDNA samples. As a positive control for the PCR re-
actions of HPRT and type I IFN receptors, human cDNA
[from BON cells (Vitale and others 2006)] was amplified in
parallel with the cDNA samples.
HUVEC and HMVEC-L cells were grown on Thermanox?
coverslips (Nunc, Naperville, IL) fixed with acetone and
subsequently incubated for 30min at room temperature with
antibodies to human AR-1 (rabbit polyclonal antibody; Santa
Cruz Biotechnology, Santa Cruz, CA) or AR-2c (monoclonal
antibody, Dr E. Croze, Berlex Biosciences, Richmond, CA)
subunits. Standard streptavidin-biotinylated alkaline phos-
phatase (IL Immunologic, Duiven, The Netherlands) was
used according to the manufacturer's recommendations to
observe the bound antibodies. Negative controls for the im-
munohistochemistry were included by omission of the pri-
mary antibody.
Proliferation assay
After plating of cells (20,000 cells per well HMVEC-L and
10,000 cells per well HUVEC experiments) on 24-well plates
(Corning, Schiphol-Rijk, The Netherlands), the plates were
left in an incubator for 24h for the cells to adhere to the
bottom of the wells. After refreshing the medium, com-
pounds were added. The medium was refreshed at day 3
and compounds added again. After 6 days the cells were
harvested and DNA contents were measured. Total DNA
contents, representative for the number of cells, were mea-
sured using the Hoechst 33258 reagent as published previ-
ously (Hofland and others 1990).
DNA fragmentation assay
After plating 100,000 cells/well for HUVEC and 200,000 for
HMVEC-L on a 24-well plate, cells were left overnight to
Page 3
adhere. After refreshingthe medium, compounds wereadded.
After incubation for 24h DNA fragmentation (apoptosis) was
assessed using the Roche Cell Death Detection ELISA Plus?
(Penzberg, Germany). This assay is based on a quantitative
monoclonal antibodies directed against DNA and histones,
respectively. This allows the specific determination of mono-
and oligonucleosomes in the cytoplasmatic fraction of cell ly-
sates, typical for apoptosis. The standard protocol supplied by
the manufacturer was used. By both light microscopy and
measurement of DNA contents, we confirmed that cell num-
bers were not significantly affected during these experiments.
IFN-b expression
In baseline conditions IFN-b expression is low but detect-
able. To explore the possible induction of IFN-b expression,
cells were plated and refreshed as described for the apoptosis
assay. Poly I:C, double-stranded RNA, a potent activator of
IFN-b expression (Field and others 1972), was added at a
concentration of 100mg/mL and samples were collected after
2 and 4h as described in the Quantitative RT-PCR section.
Cell cycle analysis by fluorescence-activated
cell sorting
Cells (0.8–2?106) were plated in 75cm2flasks. After 3
days, the medium was changed with a fresh medium (con-
trol group) or with a fresh medium plus IFN-a or IFN-b at
the concentration of 1,000IU/mL. After 3 days of incubation
(with a confluence of about 60%–70%), cells were harvested
by gentle trypsinization and prepared for propidium iodide
(Sigma-Aldrich) staining as published previously (Vitale and
others 2006). The stained cells were analyzed on a FACSca-
libur flow cytometer (Becton Dickinson, Erembodegem,
Belgium) using CellQuest Pro Software.
Statistical analysis
All experiments were performed in quadruplicate, except
for RT-PCR and fluorescence-activated cell sorting (FACS),
which were performed in duplicate, and at least twice with
comparable results. For statistical analysis, GraphPad Prism?
4.0 (GraphPad Software, San Diego, CA) was used. One-way
analysis of variance, followed by multiple comparison Tukey
test, was used to determine statistical significance, defined as
P<0.05. All data are shown as mean?standard error of the
mean. Statistical significance is marked with an asterisk. Bars
represent means, and whiskers, standard error of the mean.
Expression of AR1 and all the variants of AR2 mRNA
have been evaluated by quantitative RT-PCR, as shown in
Fig. 1A. In both cell lines, receptor subtypes AR1 and AR2
a/b/c have comparable distribution; overall expression lev-
els are not significantly different between cell lines (P>0.05
for all subtypes). Immunostaining of both cell lines shows
the presence of AR1 and AR2c on endothelial cells (Fig. 1B–
G). These 2 subtypes jointly represent the active signal
transducing complex. Both cell lines show a comparable
staining and distribution of receptors: whereas AR1 staining
is diffuse and membrane bound and/or cytoplasmic, AR2
staining is granular and appears to be more cytoplasmic than
membrane bound.
Endothelial cell proliferation
the proliferation of HUVEC and HMVEC-L, IFN-b (10–
1,000IU/mL) treatment shows a statistically significant cell
growth inhibition in both cell lines (Fig. 2A, B) in a dose-
dependent manner [HMVEC-L: the half maximal inhibitory
concentration [IC50]¼60IU/mL (95% confidence interval:38–
84); HUVEC: 90IU/mL (78–122) P>0.05]. Population dou-
blingtimesfor HUVECare41h,and81hafter IFN-b treatment
(1,000IU/mL), for HMVEC-L 81 and 384h, respectively.
DNA fragmentation
Stimulation of apoptosis by IFN-b has been reported to be
one of the main mechanisms involved in the inhibition of
proliferation, particularly in cancer cells (van Koetsveld and
others 2006; Vitale and others 2006). Therefore, we measured
DNA fragmentation after 24h of IFN treatment (Fig. 2C, D).
Surprisingly, IFN-b inhibits DNA fragmentation in a dose-
dependent manner [HMVEC-L: IC50¼51IU/mL (95% con-
fidence interval: 26–97); HUVEC: 105IU/mL (39–281) at
1,000IU P<0.001 for both cell lines]. IFN-a did not have a
significant effect on apoptosis at a similar dose (P>0.05).
After 24h of incubation with IFN-a or IFN-b, there was no
change in cell number (DNA measurement; data not shown).
IFN-b expression
Figure 3 shows the IFN-b expression levels with and
without poly I:C treatment. Poly I:C binds to a toll-like re-
ceptor (TLR-3) and is a known inducer of an antiviral re-
sponse in cells (Basu and Fenton 2004). Under baseline
conditions, IFN-b expression is very low but detectable. After
2h of Poly I:C treatment there was a dramatic increase of
IFN-b mRNA (HUVEC: P<0.001 and HMVEC-L: P<0.05),
followed by a decrease after 4h (HUVEC: P<0.05 and
HMVEC-L: P>0.05). The increase of IFN-b mRNA during
stimulation was significantly higher in HUVEC than in
Cell cycle effects
The effects of treatment with IFN-a (1,000IU/mL) and
IFN-b (1,000IU/mL) on the cell cycle phase distribution have
been evaluated in HUVEC and HMVEC-L cells. While IFN-a
does not affect S-phase phase cell counts after 72h of incu-
bation, IFN-b induces a significant accumulation of both cell
lines in S phase compared to the control. The proportion of
cells in G0-G1-phase is not modified by either treatment in
HUVEC and HMVEC-L.
Endothelial cell proliferation is an essential step in an-
giogenesis. Therefore, the inhibition of this process may re-
sult in antiangiogenic effects (Carmeliet 2003; Tonini and
others 2003; Taraboletti and Giavazzi 2004). In literature
there are contradictory results about the effects of IFN-a and
IFN-b on endothelial cells, showing both inhibitory (Ruszc-
zak and others 1990; Hong and others 2000; da Silva and
EFFECTS OF INTERFERONS a/b
Page 4
others 2002; Pammer and others 2006; Wada and others
2009) and stimulatory effects (Cozzolino and others 1993;
Mintzer and others 1998; Gomez and Reich 2003) on prolif-
eration. These apparent discrepancies between the different
studies may be related to the culture conditions, concentra-
tions, and the synthetic IFN subtypes used. The aim of the
present study was to evaluate the antiproliferative effects of
IFN treatment on endothelial cells, and especially the dif-
ferences between IFN-a and IFN-b subtypes. Parallel to
HUVEC, the most commonly used model for endothelial
cells, we also studied HMVEC-L because of their adult mi-
crovascular origin, possibly resembling tumor endothelial
cells more accurately. A completely different endothelial cell
model next to HUVEC adds to the biological validity of our
For the first time, type I IFN receptors are identified im-
munohistochemically on both HUVEC and HMVEC-L. Both
cell lines have similar expression of these receptors. Func-
tional activity of the receptor complex is shown by the re-
sponse to IFN treatment. Both cell lines can promptly express
IFN-b when stimulated by poly I:C. Presumably this is
similar to in vivo pathophysiological circumstances, such as
during viral infections.
We assessed growth of endothelial cells after IFN treat-
ment. Both cell lines HUVEC and HMVEC-L show a potent
inhibition of proliferation by IFN-b. IFN-a is not able to in-
hibit the growth of endothelial cells at a concentration
ranging from 10 to 1,000IU/mL. Additionally, cell cycle
analysis by FACS shows an increase in S-phase after 72h of
IFN-b treatment, for both cell lines (Fig. 4). This represents a
cell-cycle block in late S-phase, as has been reported before in
several tumor cell lines as well (van Koetsveld and others
2006; Vitale and others 2006, 2007). IFN-a does not induce
any effect on cell cycle distribution at this dose range. The
number of cells in G1/0 seems unaffected after IFN-b treat-
ment, considering that we showed that the apoptosis, mito-
AR1 and AR2a/b/c mRNA
(Copies/HPRT) (P>0.05 for
AR1, (D) HUVEC AR2c, (E)
HMVEC-L AR2c, (F) HUVEC-
negative control, (G) HMVEC-
L-negative control.
transferase; HUVEC, human
umbilical cord
cells; HMVEC-L, human mi-
cro vascular lung endothelial
(A) Expression of
Page 5
sis, and growth rates are decreased in the treatment group
(Fig. 2). The increase in S-phase can be compensated by a
decrease in the aforementioned phases. Possibly, only after
prolonged incubation, a decrease in G0/1 may be observed.
Since our cells were not synchronised, more and more cells
arrive at S-phase and are blocked during IFN-b treatment,
leading to accumulation in S- and decrease in G1/0 and
other phases.
Both HUVEC and HMVEC-L show a dose-dependent
decrease in apoptosis after IFN-b treatment. This was unex-
pected and in contrast with our findings in tumor cells (van
Koetsveld and others 2006; Vitale and others 2006, 2007). An
analogous effect was noted by Pammer et al. (Pammer and
others 2006). They found that IFN-a could prevent apoptosis
induced by serum starvation in HUVEC, using an identical
cell death detection ELISA. They also studied endothelial cell
apoptosis under normal culture conditions, without observ-
ing a significant effect on apoptosis by IFN-a.
Wada and others (2009) recently showed a moderate in-
hibitory effect of high-dose IFN-a (10,000IU) on cell growth
of HUVEC cells. The following were observed: (1) a slight
suppression of apoptosis was reported during IFN-a treat-
ment (apoptosis in control versus IFN-a: 11 versus 7.6%); (2)
IFN-a induced accumulation of HUVEC cells in S-phase after
24h of incubation. In these experiments a less proliferative
culture medium was used possibly leading to lower dose
thresholds for IFN effects. However, a proliferative medium
may more accurately resemble a highly angiogenic tumor
environment. Inadvertently, HMVEC-L must be cultured in
EBM-2MV?medium; in a less proliferative environment
these cells do not proliferate consistently. For matters of
comparability it was necessary to culture HUVEC in this
medium as well.
As mentioned above, Wada and others (2009) reported a
moderate inhibitory effect on cell proliferation using high-
dose of IFN-a (10,000IU/mL) and only a slight effect with
lower doses. We did not observe a significant inhibitory ef-
fect on cell proliferation at 1,000IU/mL of IFN-a, but we did
Dose Interferon b b
DNA Contents (% control)
DNA Contents (% control)
on proliferation after 6 days (dotted line: HMVEC-L). (C) Effects of IFN-a and IFN-b treatment on DNA fragmentation
(apoptosis) after 24h. (D) Dose-dependent effects of IFN-b treatment on DNA fragmentation after 24h. (*P<0.05). IFN,
(A) Effects of IFN-a and IFN-b treatment on proliferation after 6 days. (B) Dose-dependent effects of IFN-b treatment
(0h), and after 2 and 4h of poly I:C treatment. In baseline
conditions, expression is low but detectable. After stimulation
by poly I:C, a vast increase can be observed in IFN-b mRNA
expression in HUVEC (*P<0.001) and HMVEC-L (*P<0.05).
From left to right: 0, 2 and 4 hours for both cell lines.
Expression of IFN-b mRNA, baseline conditions
EFFECTS OF INTERFERONS a/b
Page 6
not evaluate the activity of this cytokine at higher supra-
clinical dose. In addition, we observed a potent inhibitory
effect on HUVEC and HMVEC-L cell proliferation after in-
cubation with IFN-b. This could partially be explained by the
fact that although IFN-a and IFN-b interact with the same
receptor, IFN-b has a binding affinity *10-fold higher than
IFN-a (Johns and others 1992).
Apoptosis and the cell cycle are considered to be inti-
mately coupled (Vermeulen and others 2003). Indeed both
processes share many of the controlling pathways. However,
according to the classical proliferation–survival model (Bla-
gosklonny 2003) apoptosis should increase when prolifera-
tion is inhibited. Our results show a contradictory relation. In
a study by Tanaka and others (1998) it was shown that IFNs
are key mediators of apoptosis in virus-infected cells. Heal-
thy cells could not be induced to undergo apoptosis unless
they were co-stimulated. Similar co-stimulatory effects were
seen in adhesion molecule expression (inter-cellular adhesion
molecule 1 [ICAM-1] and vascular cell adhesion molecule 1
[VCAM-1]) after IFN-b treatment; endothelial cells were only
stimulated by IFN-b to express these molecules when co-
stimulated by tumor necrosis factor-a (Kobayashi and others
2008). Takaoka and Taniguchi (2003) propose that even under
physiological circumstances, IFNs play a critical role in
to external and internal signalling. In pathophysiological cir-
cumstances, tumor and virus-infected cells have intrinsic and
extrinsic co-stimuli; this makes them susceptible to the cyto-
toxic effects of IFN-b (Tanaka and others 1998; Takaoka and
Taniguchi 2003; Takaoka and others 2003). HUVEC and
HMVEC-L may lack these co-stimuli.
In conclusion, there is clear evidence that IFN-b inhibits
proliferation of endothelial cells. The main cause of growth
inhibition is a cell-cycle block in S-phase. This may partially
explain the antiangiogenic effects as seen in vivo after IFN
treatment, although paracrine effects also appear to be in-
volved (Wang and others 2003; Wada and others 2009). This
hypothesis should be further strengthened by future in vivo or
in vitro studies (such as endothelial cell migration or tube
formation on matrigel). Furthermore, IFN treatment is not
directly cytotoxic or pro-apoptotic for endothelial cells, in
contrast to tumor cells (van Koetsveld and others 2006; Vitale
and others 2006, 2007). Currently, IFN-a is the most widely
used type 1 IFN in clinical-oncological treatment protocols
(Jonasch and Haluska 2001; Picozzi and others 2003). Since
our data suggest a more potent effect of IFN-b treatment,
further in vivo and clinical research on this point is warranted.
Author Disclosure Statement
No competing financial interests exist.
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a abbaabbaabbaabb
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Address correspondence to:
Dr. Joris Erdmann
Department of Surgery
Erasmus MC
Dr. Molewaterplein 40
3015 GD Rotterdam
The Netherlands
E-mail: firstname.lastname@example.com
Received 5 October 2009/Accepted 13 October 2010


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text:
USB OTG adapter cable doesn't work as promised
Device: ASUS Google Nexus 7 16GB.
OS: Got it with Android 4.1.1 that immediately updated to 4.1.2. Now Google sent an update to 4.2.
Problem: To get an USB stick running at the device as a "drive" (shown as folder for opening it).
Vendor at AMAZON.
Video at YouTube.
The adapter doesn't work as suggested. Even a direct connection of a stick (several typed tested!) doesn't work... - with or without "StickMount".
However 1: All WLAN drives from several PCs are shown (that's normal).
However 2: I get a keyboard and a mouse running properly.
But I need/want my sticks shown by the NEXUS.
However 3: Sure, I can connect the Nexus to one of my PCs where also Sticks are plugged and exchange any datas in any way.
Thanks in advance for any good advice.
Edited by: consulting ( )
@ Seamus,
at first: THANKS.
ROOT? - I'm s simple user. I purchased the adapter, detected the StickMount software and did install it by the offered procedure. - That's all I was able to do.
Is there a special way to install StickMount? (At present I'm using the update version Android 4.2.)
BTW: When I use the WLAN for seeing the other PCs and connect a stick to my notebook and release the stick in the notebook explorer I can see and open the notebook and the connected/plugg
Accepted Answer
Did you root your device? Without root stickmount does not work.
use nexus media importer it works without read but the nexus can only read media and not write (btw the nexus media importer is not free you have to buy it if you want to look at pictures etc.. from the sticks etc.. sorry.)
oh and just search the internet for android root but you lose waranty if you root :/


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Rising to the latest technology challenges in animal research
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King's is one of 17 R&D consortia to be awarded up to £100,000 each in a bid to solve five of the pharmaceutical industry's biggest drug discovery and development challenges, where the replacement, reduction and refinement of animals in research (the 3Rs) is the ultimate goal.
The CRACK IT Challenges programme, led by the UK's National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), is an open innovation platform set up to solve scientific and business problems with a 3Rs theme.
The latest round of preclinical challenges, identified with companies such as GlaxoSmithKline, Pfizer and Roche, focus on the development of new non-animal drug safety assays, a virtual infectious disease research platform and an approach to reduce animal use in the development of chronic inflammatory disease treatments.
Phase 1 finalists will have six months to develop the most successful proof-of-concepts for each of the five preclinical challenges, with the most successful group winning a three-year contract per challenge of up to £1m for further development and validation. The approach is intended to improve the chances of viable products emerging onto the market from the programme.
Dr Ben Forbes, Reader in Pharmaceutics at King's, will lead a team looking at ways to expediate the development of inhaled medicines for the treatment of chronic inflammatory diseases of the airways, such as asthma and chronic obstructive pulmonary disease, which remain areas of considerable unmet medical need. Few new drugs have made it to the clinic during the past 50 years, with many that perform well in preclinical animal studies failing in humans due to lack of safety and/or efficacy. This Challenge aims to enable the longitudinal and non-invasive assessment of inflammation associated with drug toxicity in the same animal, rather than using multiple animals, to reduce animal use by up to 90% at certain stages of drug discovery and development. Data obtained would therefore be more reliable and less variable and would lead to earlier go/no-go decisions on a drug candidate that may otherwise fail later in development after further animal tests had taken place. This project is sponsored by Huntingdon Life Sciences, Pfizer and GlaxoSmithKline.
The competition was run through the UK's innovation agency, the Technology Strategy Board, as part of the Small Business Research Initiative - with a total budget of £7 million. This includes £2 million from the government-backed Technology Strategy Board - a doubling of its contribution compared with the previous year.
Dr Vicky Robinson, Chief Executive at the NC3Rs, said: 'In many cases drug-induced toxicity results in a significant number of new drugs failing before they reach the market place; often this is not identified until animal studies have taken place. By developing more predictive technologies and approaches for use in the earliest stages of drug development, industry scientists will be better equipped to identify whether a new drug is suitable for later-stage testing in animal studies and humans. This is not only more cost-effective, but has the potential to significantly reduce the number of animals needed overall.'
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BRP's four-wheel EXIT Concept aimed at Gen Y
By Mike Hanlon
August 31, 2006
September 1, 2006 Gen Y consumers (14 to 23 year olds) are a complex lot, an intriguing market to attract. They are environmentally-conscious, thrill-seeking, competitive and they like to define their own counter culture. In seeking to address these fickle consumers, Bombardier Recreational Products (BRP) has developed an interesting new and innovative motorized recreational vehicle aiming to provide the highest fun factor with the lowest amount of horsepower. The BRP EXIT, is a 4-wheel crossover vehicle, positioned somewhere between a trial motorcycle and a BMX bicycle. Powered by a low horsepower Rotax 4-TEC engine, it is lightweight, small and simple with a high fun factor. To make it light and easier to handle, the frame of the EXIT is a minimalist steel centre beam known as a Surrounding Spar Technology (SST) frame, like the ones used on BRP's all-terrain vehicles, supporting an equally minimalist polyethylene body structure.
Its distinctive character comes mostly from its innovative rear-entry platform. It creates a new kind of man-machine interaction, resulting in a new way of riding and "working" the vehicle. It allows the rider to discover new tricks and continually improve his or her riding skills. The styling and graphics are non-conventional, motivated by Gen Y youths' fondness for the trendy "dark side". Ultra-basic geometric language was used to emphasize the simplicity of the concept and the colour-scheme is borrowed from classic military vehicles. The EXIT is on the forefront of eco-friendly, low-impact motorized performance products. It is a project conceived as part of BRP's design team ongoing search for innovative solutions.Share
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One of my all time favorites, that and Drunken Angel are perfect to see together. Get them both. Young and handsome Mifune playing two very opposite roles. Read morePublished 1 month ago by Julie Merwin
I find it rather telling that Akira Kurosawa didn't really like this film. He talked about how it was lacking in depth, and how despite the fact that it had the technique, it was... Read morePublished 4 months ago by Andrew Ellington
One of his best! One of the few movies that shows post war Japan.Published 6 months ago by Leonard Roos
"Stray Dog" is possibly the greatest suspense noir ever made. It's a detective police story about a young police officer who gets his gun stolen while he's traveling on a... Read morePublished 9 months ago by B. Adducchio
`Stray Dog' is Akira Kurosawa's 'Ladri di biciclette'. But, the movie doesn't reach the top level of Vittorio De Sica's masterpiece. Read morePublished 19 months ago by Luc REYNAERT
This is a good detective story with a dramatic ending but what makes it really interesting are two other things. Read morePublished on March 10, 2013 by The Curmudgeon
Filmed a few years after the end of the war in the still bombed-out Japanese cities, this excellent movie depicts a police officer pursuing a killer.Published on December 26, 2012 by Paul Mccarthy
I first saw this film on TCM. once again, Kurasawa gave us a masterpiece. one of the most interesting aspect of this film is that it was shot within a few years of the end of World... Read morePublished on December 11, 2012 by Richard Ranta
"Nora inu" ("Stray Dog") from 1949 was directed by undoubtedly one of the greatest 'auteurs' of all-time, Akira Kurosawa. Read morePublished on June 1, 2012 by CARLOS ROMERO


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Hummer Repair in Romeoville
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Create a Profile For Your Black Business & Events And We'll Do The Rest!
THE Netflix of #BLACKBUSINESS #URBANEVENTS PROMOTIONS. Create a Profile at WWW.BLACKFOLKHOTSPOTS.COM for us to start promoting your business.
How many of you are actively authoring a blog related to a business niche or just personal topics? I've been blogging since 2002 and I try to visit as many black blogs as possible in an effort to provide other bloggers with traffic and to share information that I find helpful with my own readers. I have two blogs: IcemanBaldy.com and TheHustleTownChronicle.com.
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Gourmet Glamping: a Chef, a Meal and a Whole Lot of Fun
on June 26th, 2014Print This Post
Glamping on San Juan Island
by Foodista.com
Add this to your summertime bucket list: Gourmet Glamping. If you're wondering what "glamping" is, think camping but without actually roughing it. Sleep in a comfortable bed in a wood-floored canvas tent and eat really, really good food that you cook over an open fire with a guest chef as your guide. Food truly does taste better when it's cooked outdoors, especially when paired with exceptional wines and an amazing group of people.
Continue reading "Gourmet Glamping: a Chef, a Meal and a Whole Lot of Fun" »
The 25 Best San Francisco Restaurants for Summer 2014
on June 25th, 2014Print This Post
The Best San Francisco Restaurants to Eat at Right Now
Every summer, San Francisco comes alive with culinary possibilities, both new and old. There are the neighborhood standbys, of course, but the refreshing summer season tends to call for something a bit more exciting. So dive into Eastern European fare deep in the Mission, carouse over bay views and New Orleans-themed plates from a Slanted Door alum, then make your way to Berkeley for some of the city's best regional Mexican fare. Summer is here. Presented in alphabetical order, these are GAYOT's 25 best restaurants in San Francisco to eat at, right now.
Continue reading "The 25 Best San Francisco Restaurants for Summer 2014" »
The 25 Best New York Restaurants for Summer 2014
on June 19th, 2014Print This Post
The Best New York Restaurants to Eat at Right Now
Summer is a great time to be in New York City, especially for anyone intent on eating well. Patios open up for all-day dining, outdoor rooftop lounges offer sweeping views of the city at night and there always seems to be an endless variety of new restaurants to enjoy. This summer, choose to experience Mediterranean flavors from a celebrity chef, tasting menus at a grocery store in Brooklyn or searingly spicy Thai food from a dingy restaurant in Red Hook. Presented in alphabetical order, these are GAYOT's 25 best restaurants in New York City to eat at, right now.
Continue reading "The 25 Best New York Restaurants for Summer 2014" »
10 Must-Visit Restaurants in Orange County
on June 13th, 2014Print This Post
Putting the "Oh" in OC
by Rich Manning
After decades of languishing in the shadows of Los Angeles, the Orange County food scene has as of late been able to craft a strong identity all its own. These days, one is never too far from finding a restaurant that is driven by innovative technique, a desire to build the perfect flavor profile and an ambience that coaxes guests into staying a little while longer.
Continue reading "10 Must-Visit Restaurants in Orange County" »
Top 5 BBQ Styles
on June 06th, 2014Print This Post
Finger-Licking Barbecue from Around the World
by Nick Winfrey
Barbecue is more than just a method of cooking. It's a social event, a source of regional pride, and, for many sticky-fingered aficionados around the world, a way of life. Continue reading "Top 5 BBQ Styles" »
Beer vs. Wine Smackdown Round 2
on May 29th, 2014Print This Post
A Food and Drink Pairing Competition
by Bob Barnes
"The filet mignon will go very well with a dry Cabernet or a spicy Belgian saison." Until recently you would not have heard a sommelier make the latter part of that statement, but times are changing. Proof of the leveled playing field for beer and wine was the "Beer vs. Wine Pairing Smackdown" held on May 21 at miX amidst the stunning views from the 64th floor of THEHotel at Mandalay Bay. The event pitted beer against wine in a five-course pairing dinner, each dish served with a beer and wine selected by the respective teams. Audience participation involved voting for the libation that paired best with each course; and selecting "Team Beer" or "Team Wine" buttons to wear, some choosing to don both out of a fear of commitment.
Continue reading "Beer vs. Wine Smackdown Round 2" »
Exploring the Côtes de Bordeaux
on May 26th, 2014Print This Post
Introducing one of France's newest appellations
by Michelle Kwan
France's famous Bordeaux region comprises numerous appellations and sub-regions, from St-Emilion, Pomerol and Médoc to Entre-Deux-Mers and Sauternes. In 2007, the AOC Côtes de Bordeaux was created, combining the "terroirs" of Blaye, Cadillac, Castillon and Francs, in order to distinguish this unique region from its neighbors. The appellation also gives winemakers within the region the flexibility to blend wines from different areas within the appellation.
Continue reading "Exploring the Côtes de Bordeaux" »
L.A. Restaurants Celebrating Cinco de Mayo
on May 02nd, 2014Print This Post
Eat, Drink and Save Dinero
With Cinco de Mayo falling on a Monday this year, some of the city's best eating and drinking spots have upped the ante with a three-day weekend full of deals, drinks and Mexican food.
West Hollywood's Tortilla Republic is hosting their own block party on Sunday, May 4th. The booming affair runs from 2 p.m.-10 p.m. and will feature DJs, a live mariachi band and VIP suites complete with bottle service. Of course, $6 margaritas and crispy chicken flautas will also be available.
Continue reading "L.A. Restaurants Celebrating Cinco de Mayo" »
Iron Chef Jose Garces Opens Volvér in Philadelphia
on April 22nd, 2014Print This Post
Iron Chef Jose Garces Will Take the Stage at His Newest Restaurant in Philly
by Robert DiGiacomo
If chefs are the new rock stars, then Jose Garces is inviting you to a very intimate culinary jam session.
The Philadelphia-based "Iron Chef" recently opened Volvér, a high-end restaurant located in the city's Kimmel Center for the Performing Arts. The latter is home to the city's acclaimed orchestra and a presenter of jazz, dance, classical and pop concerts.
Continue reading "Iron Chef Jose Garces Opens Volvér in Philadelphia" »
French Rosés on the Rise
on April 15th, 2014Print This Post
Provence in the City
by Michelle Kwan
Crisp, chilled rosé is commonly considered to be a warm weather drink, but that didn't stop Provençal rosés from coming to New York City early this year. To be precise, they arrived ten days before spring at Lafayette restaurant in NoHo on an overcast Monday in March. This year's "Provence in the City" tasting and seminar featured more than 60 different wines. Six were paired with four Provençal-influenced courses from Lafayette.
Continue reading "French Rosés on the Rise" »
« Newer Posts — Older Posts »


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10,023pages on
this wiki
this wiki
Category page
This category is for glitches in the Pokémon video games. Some of these glitches are known to corrupt save games and mess up the graphics in the game.
Pages in category "Glitches"
" Glitches in Pokémon games, both major and minor, have been a mainstay throughout the existence...
Pokémon Glitches
" Charizard 'M is a hybrid of 'M in Pokemon Red and Blue. It can only be obtained by trading the...
Charizard 'M
" A is a dual-type Normal/Normal Glitch Pokémon found in Pokémon Red and Blue. It can be...
A (Glitch Pokémon)


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2013 Summer Camps
Robert Morris offers a multitude of camps in a variety of sports throughout the summer months. Come join us at our various camps this summer! For information on any of RMU's upcoming 2013 camps, please click on the respective link below. As more camps become available, look for more links.
• 2013 Nike Boys Lacrosse Summer Camp at Robert Morris
• 2013 Coach Sal Buscaglia Girls Basketball Camp at RMU


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Book your room at Moulin de Vigonac
Grand comfort double roomMax Occupancy: 2 guests (up to 1 infant)
Grand comfort double room with terraceMax Occupancy: 2 guests (up to 1 child, 1 infant)
Grand comfort room with terrace, ground floorMax Occupancy: 2 guests (up to 1 child, 1 infant)
Luxury Double Room, BalconyMax Occupancy: 2 guests (up to 1 child, 1 infant)
Luxury LoftMax Occupancy: 2 guests (up to 1 child, 1 infant)
Comfort Double Room, Courtyard ViewMax Occupancy: 2 guests (up to 1 child, 1 infant)
Luxury Double Room, TerraceMax Occupancy: 2 guests (up to 1 child, 1 infant)
4-star hotel in Brantome with an outdoor pool
Situated on the riverwalk, this hotel is close to Brantome Abbey. Area attractions also include Chateau de Bourdeilles.
Hotel Features
In addition to a restaurant, Moulin de Vigonac features an outdoor pool. Other amenities include complimentary wireless Internet access and a porter/bellhop.
Room Amenities
Flat-screen televisions come with satellite channels. Guestrooms also feature complimentary wireless Internet access, complimentary high-speed (wired) Internet access, and premium bedding.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
The hotel offers a restaurant. High-speed wireless Internet access is complimentary. This Brantome hotel also offers an outdoor pool, a terrace, and tour/ticket assistance. Onsite self parking is complimentary.
- 24-hour front desk
- Total number of rooms - 10
- Number of floors - 2
- Breakfast available (surcharge)
- Concierge services
- Express check-in
- Express check-out
- Tours/ticket assistance
- Free WiFi
- Beach/pool umbrellas
- Multilingual staff
- Garden
- Terrace
- Porter/bellhop
- Restaurant
- Free self parking
- Number of buildings/towers - 3
- Outdoor pool
- Free use of nearby fitness center
Available in all rooms: Free WiFi , Free wired high-speed Internet
Available in some public areas: Free WiFi
Free self parking
Room Amenities
- Premium bedding
- Furnished balcony or patio
- Phone
- Minibar
- Private bathroom
- Designer toiletries
- Bathrobes
- Free toiletries
- Hair dryer
- Slippers
- In-room safe
- Desk
- Blackout drapes/curtains
- Satellite TV service
- Flat-panel TV
- Free WiFi
- Free wired high-speed Internet
- Individually furnished
- Individually decorated
- Connecting/adjoining rooms available
- Free cribs/infant beds
Where to Eat
Continental breakfasts are available for a surcharge and are served each morning between 8 AM and 10:30 AM.
Onsite venue - Overlooking the garden, this fine-dining restaurant specializes in French cuisine and serves breakfast and dinner. Reservations are required. Open select days.
Nearby Things to Do
The recreational activities listed below are available either on site or nearby; fees may apply.
- Golfing nearby
- Kayaking nearby
- Bicycle rentals nearby
- Hiking/biking trails nearby
- Golf lessons available nearby
- Boat tours nearby
Hotel Policies
Check-in time starts at 3 PM
Check-in time ends at 10 PM
Special check-in instructions:
Check-out time is 11 AM
Payment types
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
No pets or service animals allowed
- A tax is imposed by the city: EUR 1.32 per person, per night. This tax does not apply to children under 12 years of age.
- Fee for continental breakfast: EUR 17 for adults and EUR 8 for children (approximately)
We should mention
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation. No pets and no service animals are allowed at this property.
National Rating
This hotel has received its official star rating from the French Tourism Development Agency, ATOUT France.


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Shaun Murphy thrashes Neil Robertson to win Masters
- Other Sports
- January 19, 2015, 09:35
Shaun Murphy became the 10th man to win the Triple Crown after thrashing Neil Robertson 10-2 at Alexandra Palace to win the Masters. Robertson, the wo(...)
Stephen Hendry considering World Championship comeback
- Other Sports
- January 16, 2015, 10:22
Stephen Hendry has confirmed he could come out of retirement and return to snooker in time for the World Championship in April.The 46-year-old Scot wa(...)
Ronnie O'Sullivan breaks Hendry century record
- Other Sports
- January 15, 2015, 14:54
Ronnie O'Sullivan claimed one of snooker's great records on Thursday as he began his Masters quarter-final against Marco Fu. The 39-year-old fired in (...)
Ronnie O'Sullivan signs off fourth round win with a 147
- Other Sports
- December 4, 2014, 23:10
Ronnie O'Sullivan finished off with a brilliant 147 break as he stormed into the quarter-finals of the Coral UK Championship with a 6-0 demolition of(...)
Ireland's David Morris causes huge shock with victory over Mark Selby
- Other Sports
- November 29, 2014, 20:30
Kilkenny's David Morris caused a huge shock at the Coral UK Championship when he knocked out world champion Mark Selby. Selby, who became a father for(...)
Ronnie O'Sullivan set for UK Championship despite injury
- Other Sports
- November 26, 2014, 12:39
Ronnie O'Sullivan is set to begin his Coral UK Championship campaign on Thursday, despite suffering a nasty ankle injury. O'Sullivan is targeti(...)
Jimmy White reveals crack habit
- Other Sports
- November 3, 2014, 18:37
Six-time World Championship finalist Jimmy White has told of his battle with crack cocaine addiction. The 'Whirlwind' (52) became a huge fans' favouri(...)
Seamus Heaney first editions, rare Belfast whisky, comic books and a famous snooker trophy for auction
- Fine Art & Antiques
- September 7, 2013, 00:00
Whyte's auction of "History, Literature and Collectibles" in Dublin next Saturday features a selection of first editions by Seamus Heaney, including a(...)
O'Sullivan extends lead over Hawkins at World Snooker Championship
- Other Sports
- May 5, 2013, 23:27
Barry Hawkins was rising to the challenge of his first Betfair World Championship final last night as he and Ronnie O'Sullivan produced a high-(...)
Selby dumped out of World Championship by Hawkins
- Other Sports
- April 27, 2013, 20:29
Weary world number one Mark Selby staggered to a dismal Crucible defeat as his dream of completing snooker's 'triple crown' was dashed by a stu(...)


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Family Getaway to the Valley of the Sun: Top 10 Things to Do in Phoenix
From art museums to hot air balloon rides – the top ten things to do on your next visit to Phoenix, Arizona.
From art museums to hot air balloon rides – the top ten things to do on your next visit to Phoenix, Arizona.
Incorporate those colored Easter eggs into easy recipes.
It's not quite blockbuster time yet but there are some good new releases to check out!
Stock up on staples and basics for effortless style.
Here are 5 great ideas to ways to incorporate cabbage to your recipes year round.
Summer is on the horizon and we've got pool tips to keep your children safe.
Can technology, especially electronic games, also help children develop better people skills?
Social and emotional learning is becoming more of a focus in our schools.
Pack your suitcase! This is a great time of year for another family adventure!
In addition to your home, subject your workstation to a little spring cleaning.
A parent's guide to the Harry Potter series.
Enjoy your favorite coffee shop snack at home.
An interview with 19-year-old Juliana Lyons about a video she edited and produced.
Jennifer Garner is reclaiming her acting career after focusing on family.
Smart ways to keeps your waves healthy all year long.
A delicious dessert filled with bananas!
Watch this video with a powerful message for our daughters.
Think seasonal this spring with these recipes.


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nuLOOM Serano Shag White Area Rug
This product has additional required/recommended options. To configure, add to your cart.
9' x 12' Rug Size
8' x 10' Rug Size
6' x 9' Rug Size
5' x 8' Rug Size
4' x 6' Rug Size
Special Offer:
24 Issues of Better Homes and Gardens
Included with your purchase - a $10 value (Subscription and Rebate Info)
nuLOOM Serano Shag White Area Rug
Weights & Dimensions
9' x 12' Rug Size
8' x 10' Rug Size
6' x 9' Rug Size
5' x 8' Rug Size
4' x 6' Rug Size
|Product Type: Kids Rug
|Construction: Machine made
|Technique: Machine woven; Shag and flokati; Tufted
|Primary Pattern: Solid
|Primary Color: White
|Gender: Unisex
|Lifestage: Kid
|Type of Backing: None
|Material: Synthetic
|Reversible: Yes
|Rug Pad Needed: Yes
|Eco-Friendly: No
|Outdoor Use: No
|Product Care: These rugs can be spot treated with a mild detergent and water, professional cleaning is recommended if necessary.
|Country of Manufacture: Turkey
About the ManufacturernuLOOM believes that floor coverings and art should not be mutually exclusive. Founded with a desire to push boundaries and break the rules of what is expected from an area rug, nuLOOM was created to fill the void between brilliant design and affordability. nuLOOM's creations infuses their expertise in producing high quality floor coverings with passion for nature's wonders and art. The designs encapsulate the organic beauty of the earth with the fresh and modern palette of today's fashion forward shopper. Innovative animal prints and color combinations in nuLOOM's Earth collection, bold lines and bright tones represented in the Metro collection, and the subtle elegance of the Flur collection are just some of nuLOOM's inspirational home decor pieces. nuLOOM's Flokati collection of 100% pure wool shag carpets handwoven in Greece brings back a home fashion accessory that has lasted for the past three millennia. The Flokati carpet dates back to the 5th century where they were used by Vlach shepherds in the Pindos Mountains of modern day Greece. Today Flokati's have emerged as one of the most contemporary floor coverings of the modern era. nuLOOM is proud to bring back this timeless plush rug from Greece to create a warm and inviting look in your home.
nuLOOM Serano Shag White Area Rug
Questions & Answers (5)
"Hello, is this rug pure white?" asked by Michael
"Hi, does this rug shed?" asked by Felica
"Hi, can I vacuum this rug?" asked by Samantha
"Hi, what material is this rug made of?" asked by Rachelle
"Hi, does the rug have a backing?" asked by Patty
Still have questions about this product? We're here to help
|5 star
|4 star
|3 star
|2 star
|1 star
Customer Reviews
Best. Rug. Ever.
Christina from Ann Arbor, MI – Verified Buyer
Do you have a dog? Buy this rug. A kid that hasn't yet mastered how to get their food directly into their mouth? Buy this rug. I know what you're thinking... why on earth would I buy a white rug if I have a dog or an uncoordinated mini human in my house?! I thought the same thing and, as a result, went through rug after rug when the inevitable accident would leave a messy spot on my area rugs. At first I assumed my trusty spotbot would take care of the messes, but somehow it got rid of both the stain AND the color in the carpet, leaving a nice white blotch. This is officially the only area rug I use in my formal dining room and casual eat in kitchen. If you have a spotbot or a decent carpet cleaner it's virtually indestructible. I. love. this. rug.
ok rug
Kristina from WA – Verified Buyer
Based on all the great reviews, I decided to purchase this rug. It has a nice off white color to it, but you can tell it's cheaply made. I guess that is expected for the price. I've seen much worse quality so that wasn't why I gave it three stars as I did expect a lower quality based on the price I paid. Perhaps I am sensitive to chemicals, but this rug smelled so bad and I've had it in my garage for over a month to "off-gas" it. I can't smell it anymore while in the garage, but when I get close to it I can still smell the chemicals. I am bringing it in the house next week as I bought a thick, non toxic rug pad from eco rug pads made in the US. If there is a noticeable smell I guess I am out a couple hundred dollars. Word of caution: as with so many products these days, be cautious of what goes into our products and perhaps pay a bit more for more environmentally friendly and people friendly products. It's unfortunate we have to worry about what they put in our rugs these days.
Just what I wanted
AKS from Midland, TX – Verified Buyer
This rug looks great in our living room. It anchors the leather furniture and provides great texture. As others have mentioned, it does have a very strong chemical smell. Ideally I should have let it air out before putting down and after taking the plastic off. After a few days (one with the door open), I sprayed it with Febreeze and it helped tremendously. A week later, for the most part, the strong smell is gone. I expect it will continue to get better.
Good product for the price
Amy from Tennessee – Verified Buyer
The rug looks whiter which is what I wanted. It is not as soft as I would like but, it was cheaper than most so I am okay with that. It looks very nice in my living room, however, it is VERY thin and has a very scratchy backing. You need to buy a thick rug pad to add some cushion and to protect your laminate or hard wood floor from being scratched. Overall, for the price I am happy with it. It looks great and with a thick rug pad it feels great now too. good value, good size, and looks nice! VERY thin rug with scratchy back. Must purchase a thick rug pad.
candice from wilmington, NC – Verified Buyer
I am in love with this carpet! It is beautiful and very soft and plush. I see people say its beautiful or happy for the price, you would never know it was so affordable I would have been happy if it were double the price
Nice rug
Pricilla from Aberdeen, MD – Verified Buyer
Nice rug. But I must admit it's not as full as the pic portrays. Not too skimpy either though. If u run ur fingers through it and fluff it up a bit it looks better. I bought a thick rug pad underneath so it feels much better under my feet. I have dark hardwood floors do the pad is necessary to prevent scratching. I'd suggest anyone who purchase this rug do the same since the underside us very rough. All in all I'm very happy with this rug.
Exactly what I wanted!
Valerie from North Salt Lake, UT – Verified Buyer
This rug was exactly as described. It is white, in a heavily trafficked area and I am raising two puppies! It looks great. It is lightweight, cleans beautifully, and I am pleased as punch with the fabric used....it is under a breakfast table and every stain has come right out!
love this rug
Kristen from Camas, WA – Verified Buyer
This rug is very plush. It has a bit of a smell from packaging so air out outside if possible or leave in the room with windows open. Needs a rug mat but for half price and no shipping costs...cant beat it.
White rug
Julie from Princeton, NJ – Verified Buyer
Good quality for price, although like some of the other reviewers I found the smell very strong for the first week. I bought a rug pad and that has been very worthwhile in ensuring the pile has a plush quality to it. Lovely and white if you are looking for a light rug. Perfect in our bedroom but I would not put it in a high traffic area. As it is low priced though it is something that can be replaced if it begins to show marks and wear.
Great rug!
Kathleen from Colchester, CT – Verified Buyer
Really elevates the space and matches well with our other white accents in the room. We have two cats and the rug doesn't show any fur.


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Hands-free faucets
I've read some favorable posts here about the Tapmaster foot controls for faucets, and am intrigued by a hands-free kitchen faucet operation. But I don't want a solution like the Tapmaster which puts the foot-controlled valve in series with the regular faucet valve: I want the faucet to always work normally (move the regular valve, water comes out) no matter what, with no latching or pre-opening required. I also want to be able to walk up to the faucet and use it hands-free without any prior preparation.
I'm thinking about getting foot-pedal valves (e.g. from T&S Brass) and a remote-valve kitchen faucet (there's one in the Kohler Simplice line, for example) and connecting the faucet input to both the regular valve and the foot valve with a (likely homemade) wye. Has anybody done this successfully?
Thmetcalf, have you looked at the Moen MotionSense faucets? Ours can be used just like a regular faucet by turning it on with the lever on the faucet, or I can walk up, wave my hand over it and it turns on. You don't have to do anything such as latching or pre-opening. Perhaps I am missing something you are looking for because it seems the MotionSense units may work the way you hope. Good luck!
I've got the Delta Touch faucets and they can be turned on or off manually by moving the handle or they can be turned on or off by touching the top of the faucet or the handle.
EmmaR, if you're using the motion sense the heat and speed are set to what you manually set. I usually leave the water pressure on medium (so there's not a ton of splashing) and at a warm setting. If I'm away from the faucet for a long time than it'll be cold as soon as it turns on but if I'm back and forth for awhile it stays fairly warm. I usually use the touch setting just to get it turned on if I've got something like cookie dough on my hands and after I've mostly rinsed off my hands I'll change the temp or pressure if necessary. It's REALLY convenient!
Here is a link that might be useful: Home Blog
The sensor and touch faucets do look interesting. The first drawback I imagine is that they might befuddle visitors; someone comes up to the faucet and is startled when water suddenly starts flowing, because they touched it or activated the sensor. For those of you that have these faucets, how have visitors reacted to them?
The other question is how long the sensing mechanism will last for. Will it keep working for 20 years? How repairable will it be?
The touch definitely befuddles visitors. I don't have unique visitors enough that it's a big deal, but if I did, and I was organized, I'd remember to put the handle in the off position. Remember that if they go for the handle, unless they've touched the neck or something else previously, it'll operate like normal...except that if they let go with it in the on position, it'd stay on. But since most people's habit is to turn it off, it works fine. I imagine someone were wearing long sleeves, they could inadvertently get a wet sleeve, but that'd be about it.
I used to be annoyed that visitors would turn it off. Now, I get it.
If the touch feature died, you'd still have a nice enough faucet.
I'm replacing it with a non-touch one in our reno and I know I'll miss it. But I also know if I miss it enough, we'll get a Tapmaster.
The problem we have with the sensor faucets at work is that they go off and on spontaneously and sometimes will not go on when you want them too.
We are getting a Kohler Sensate touchless faucet. I don't think it'll be a problem for visitors since they usually go for the handle which will work normally. If they inadvertently put their arm underneath the arc and trigger the sensor, they might be surprised but they are going for the water anyway so it shouldn't be a problem.
I'm not sure there is much historical info on the longevity of these faucets. As a previous poster said if the sensor dies you still have a regular faucet. As for the surprise arm drenching, I turned off the front-facing sensor on our Moen to prevent that from happening. The sensor on top is still activated for the wave-over on. I love the faucet. One more thought, the sensor control is in a separate box mounted below the sink. I am guessing that could be replaced in the future if needed.
We bought a Delta touch faucet about 18 months ago ... love the concept, but it's been a flaky experience for us - sometimes when the dishwasher starts to drain, the faucet turns on for 1 minute or so. A few times the touch system "died" and I had to pull the batteries to reset it. This was their 1st generation, so maybe they have improved ... but it was enough trouble that I'm going with Moen for our new one.
The biggest advantage of the Moen motionsense is that you can preset a temperature and that's what always comes out when you activate it via motionsense.... i.e. if you want warm water for washing your hands, that's where you set it (under the sink). The manual water selection overrides that, so you can easily go to hot or cold just by pulling the handle out.
Love my touch Delta. It's a funny thing and we had a few problems getting it working properly when it was first installed, but I really like it, especially when I'm handling raw meat (the main reason I got it). As for visitors, they are always befuddled but it's also a cool conversation piece.


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1st Street
New Port Richey
Commute to Downtown Jasmine Estates
Most errands require a car.
Score Details
1st Street has a Walk Score of 27 out of 100. This location is a Car-Dependent neighborhood so most errands require a car.
Nearby schools include Mandala Treatment Center, Genesis Preparatory School and Chasco Elementary School.
The closest grocery stores are American Food Distribution Inc, Sweetbay Supermarket and Publix Super Market at Regency Crossings.
Nearby coffee shops include Dunkin' Donuts, Dunkin' Donuts and Phil's Cafe. Nearby restaurants include Jeannie's Diner & Restaurant, Boulevard Family Restaurant and Wendy's.
Explore how far you can travel by car, bus, bike and foot from 1st Street.