text:
|Property type
|Minimum Stay
|2 - 3 nights
2 bedroom, one bath house with hardwood and tile floors throughout, tasteful decor. Bright, sunny kitchen opens out to back deck. Internet, cable TV and stereo on site. Small, landscaped, fenced yard allows for privacy and ease of use. Owner lives nearby and is available, for assistance.
I am the sole owner of the property. I'm originally from the east coast but have lived in Montana for 22 years.
Dar Armstrong purchased this bungalow in 2009
I moved to Missoula a few years ago from Bozeman to finish my graduate degree and found this great house. It's a comfortable, easy to maintain, in a quiet neighborhood but only one block from the bike trail to downtown and a straight shot around the corner to U of M. If you haven't spent time in Missoula before, you're in for a treat. There's always lots to do - concerts, theatre, lectures, etc. and it's an outdoor mecca, as well. The Clark Fork River runs through the city and then passes within walking distance of my property. If you value quality foods, the Good Food Store is a short walk from my place too. Every season is fun in Missoula, but my favorite is summer. The temperature is warm and the air is dry rather than humid. Great weather for hiking, fishing, biking, or just hanging out at our wonderful Farmer's Market. My place offers easy access to it all!
Great for long term rental!
My husband and I just spent the month of September here and had a wonderful time. The location was great for easy access via walking or biking to downtown, the Hip Strip and the University. The kitchen was cheery and well-stocked. We were visiting family and could do great entertaining outside on the back deck and in the backyard. There were a lot of nice touches -- comfy furniture and a console table so you could grab your stuff easily in the living room, big kitchen table for extra counter space, a nice desk area for the computer in the second bedroom, lots of closet space, new, super-quiet washer and dryer, AND an electric fireplace/heater that took the chill out of the air in the mornings. Dar was so accommodating -- she even let us use part of her garage to store our biking, hiking and fishing gear. We felt very happy, cozy and safe there and would definitely go back.
The house was wonderful, location was great, and Missoula has to be one of the best places to spend the summer.
Dar made things easy. The space was perfect, everything as described. Great for exploring both in and out of town.
We came to Missoula for the birth of our grandchild, intending to stay for a week or so. Due to complications, we ended up being here for almost 3 weeks. Fortunately, the house was available and the owner, Dar Hamilton, very gracious! The house really felt like our "home away from home". The furnishings were tasteful and very comfortable. Though the bedrooms are small, the main living space where we spent all our time was spacious, cozy, and warm - there are some nice touches like an electric fireplace and Netflix. We were able to host dinners for our family in the well-appointed kitchen and the location was perfect. We biked everywhere- on the river trail, into the Rattlesnake, the University, and downtown. It would be a little harder to get to these areas on foot. The neighborhood is very quiet and the Good Food Store is just a few blocks away. The house was clean and Dar was very attentive to our needs, always responding immediately to questions. I highly recommend this VRBO to couples, small families. Though you could get by without a car if you have bikes, it's nice to have one when the weather isn't cooperative. My thanks to Dar for her thoughtfulness throughout our stay!!
My wife and I stayed here with our toddler while in town for a class. The house was very clean and had everything we needed.
Regarding the owner, I can not say enough; she answered all my emails promptly, was very helpful and understanding when we decided to change the day of our arrival.
The only regret my wife and I had is that our vacation was too short.
We stayed at this house for an extended ski weekend. Lost Trail Powder Mountain is an easy drive to the south, with Montana Snowbowl just north of town. The bungalow is close to downtown where we enjoyed a delicious dinner, and is just a short walk to the Good Food Store for healthy groceries and snack items. Comfortable, well-provisioned, and tastefully furnished, this home is the ideal spot for a Montana getaway any time of year!
is located along the Clark Fork and Bitterroot rivers in Western Montana and at the convergence of five mountain ranges, thus is often described as being the 'Hub of Five Valleys'. The 2010 Census put the population of Missoula at 66,788 and the Missoula Metropolitan Area at 109,299. It sits at an elevation of 3200 feet and is considered an outdoor mecca. The city offers lots of cultural and recreational activities, from the International Documentary Film Festival, the longest running Farmer's Market to the annual Festival of the Book - there is always something fun and interesting to do in Missoula. It is located on route to Glacier National Park, Flathead Lake, and the Salish Kootenai Indian Reservation.
|Rental Period
|Minimum Price
|Additional Nights
|Weekend Night
|Monthly *
|Event Price
My Standard Rate
2 night minimum stay
for 2 nights
|Property Damage Protection
|Tax Rate
* Approximate monthly price. Actual pricing will depend on the days of the month you stay.
Please Note: The owner of this property accepts payment in the original currency specified (USD), before use of conversion tool. The conversion tool is provided for your convenience only. The owner may accept other currencies if specifically agreed in advance.


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National Mall
National Mall design competition concepts on displayComment
Designers and architects are dreaming big for a chance to improve the place sometimes called America's front yard-- the National Mall.
Bus tours restored on National MallComment
The National Park Service is bringing interpretive bus tours back to the National Mall after the previous tour operator went out of business.
Reason Rally: Thousands to convene on the National Mall in a 'Woodstock for Atheists'Comment
As many as 20,000 people plan to gather for the Reason Rally at the National Mall this weekend, united by their lack of belief in the kind of higher power promoted by most religions.
Capital Bikeshare's bikes dominated the National Mall this weekend
The red bikes were everywhere this weekend as the District resident celebrated its century-old cherry blossoms. The two new Bikeshare stations, despite initial kinks, appeared just in time.
Bikeshare comes to the Mall, just in time for cherry blossoms
The first two Capital Bikeshare stations have been installed, with three more set for the coming weeks.
Food trucks on the National Mall being pushedComment
Is that the lobster truck on the National Mall?
POLL: Should food trucks be allowed on the National Mall?Comment
There's a new push to bring D.C.'s food truck craze to the National Mall, one of the nation's most sacred sites. What do you think: should food trucks be allowed on the National Mall? Take our poll.
National Mall grass replacement being consideredComment
There may soon be less grass on America's "front yard" under plans being considered to improve the turf on the National Mall and provide space for events.
Capital Bikeshare on the National Mall vote set for March 1stComment
The Washington Post reports that a vote to install the first of five Capital Bikeshare stations near several National Mall monuments is coming soon, and that stations could be up and running by the Cherry Blossom Festival.
Akridge donates $1 million for National Mall restorationComment
The family foundation of a Washington developer is announcing a $1 million gift to help restore the National Mall's grounds and facilities.
Washington shuttle shuttle service begins at Arlington CemeteryComment
Visitors to the National Mall and to Arlington National Cemetery will have new transportation options in time for the National Cherry Blossom Festival.
Trust for the National Mall holds design competitionComment
The Trust for the National Mall is currently holding a design competition looking for ways to reinvent the space.
Yes, really: NPS proposes five Capital Bikeshare stations on the National Mall
NPS is inviting public comment on the five proposed Capital Bikeshare stations for the National Mall.
Power outage on National Mall expected Friday night for repairsComment
Power on a large area of the National Mall and Memorial Parks will be turned off Friday night to allow for repairs to damaged power lines.
National Mall ice rink reopensComment
The ice rink in the National Gallery of Art Sculpture Garden is reopening for another season of skating on the National Mall.
National Mall to get overhaul in design competitionComment
A nonprofit group that raises money to improve the National Mall is announcing a jury of architects, preservationists and other experts who will judge the first phase of a design competition to overhaul three sites on "America's front yard."
Grab shovels: Wednesday is National Fossil Day on the D.C. MallComment
Today, take your kids to National Fossil Day on the National Mall, where you can sift for fossilized remains and trade "fossil cards." Paleontologists will be present.
Glenn Neff to appear in court, planned to set off fireworks on MallComment
Glenn Neff, the man who allegedly planned to set off fireworks in a show of protest on the National Mall in August, will appear in court Friday.
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2011 Volkswagen Tiguan Nationwide
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"New Facelift 2011 VW Tiguan Sport."
I bought this mk2 Tiguan new in November 2011 and am so far very happy with it. The ride, handling and acceleration are very good for the size of the vehicle. I got the Dynaudio speaker upgrade and the sound is excellent. The only things I'm not to happy about is the front seats could be more comfortable and the boot is a little on the small si...
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2011 Volkswagen Tiguan SE 4Motion Used Cars in Orlando, FL 32822
Price: $14,999
Mileage: 35,554
Location: Orlando, FL 32822
2011 Volkswagen Tiguan S 4Motion Used Cars in Fuquay Varina, NC 27526
Price: $13,500
Mileage: 61,504
Location: Fuquay Varina, NC 27526
2011 Volkswagen Tiguan SEL Used Cars in Albuquerque, NM 87110
Price: $16,395
Mileage: 46,653
Location: Albuquerque, NM 87110
2011 Volkswagen Tiguan SE Used Cars in Highland Park, IL 60035
Price: $13,995
Mileage: 56,986
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2011 Volkswagen Tiguan SEL 4Motion Used Cars in Washington Township, NJ 07676
Price: $19,995
Mileage: 18,727
Location: Washington Township, NJ 07676
2011 Volkswagen Tiguan S Used Cars in Bridgeview, IL 60455
Price: $12,954
Mileage: 45,166
Location: Bridgeview, IL 60455
2011 Volkswagen Tiguan SEL 4Motion Used Cars in Riverside, CA 92504
Price: $15,977
Mileage: 70,763
Location: Riverside, CA 92504
2011 Volkswagen Tiguan S Used Cars in Hawkinsville, GA 31036
Price: $13,900
Mileage: 35,735
Location: Hawkinsville, GA 31036
2011 Volkswagen Tiguan Used Cars in Hamilton, NJ 08691
Price: $15,987
Mileage: 17,105
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Price: $17,500
Mileage: 28,399
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2011 Volkswagen Tiguan S Used Cars in Lewisville, TX 75067
Price: $12,997
Mileage: 56,320
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2011 Volkswagen Tiguan Used Cars in Greensboro, NC 27407
Price: $14,250
Mileage: 58,453
Location: Greensboro, NC 27407
2011 Volkswagen Tiguan S Used Cars in Warrenville, IL 60555
Price: $13,800
Mileage: 45,464
Location: Warrenville, IL 60555
2011 Volkswagen Tiguan S Used Cars in Novi, MI 48375
Price: $10,900
Mileage: 76,602
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2011 Volkswagen Tiguan SE 4Motion w/ Sunroof and Navigation Used Cars in Saint Louis, MO 63122
Price: $18,950
Mileage: 37,170
Location: Saint Louis, MO 63122
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|Property type
|Minimum Stay
|3 - 14 nights
This is a beautiful condo in building #6 that is kept very clean and well decorated in tropical decor. One of the largest 2 bedroom, 2 bath, corner units with almost 1200 sq. ft. plus a huge covered wrap around lanai with a peek a boo ocean view and views of the 13th and 14th fairways of the Kona Country Club golf course. Quiet location away from Alii drive in building 6, just steps away from the ocean.
The bright and airy living room fully opens to the lanai on 2 sides with screened sliding glass doors allowing for a wonderful fresh breeze and the sound of the pounding surf. Beautiful tropical island decor and artwork.
A previous guest commented in her review, 'This is a beautiful home is in a great location on the shore of Hawaii's Kona coast. The condo is large, very clean and well-maintained, nicely furnished, attractive and comfortable.'
The first bedroom has a comfy queen plush bed with fresh clean linens and opens to the lanai with a screened patio door. It can be closed off from the living room with a sliding wall. Ample closet space and a separate full bath with fresh linens plus all the shampoo and soaps are provided.
The second bedroom has a comfy queen bed and a twin bed, fresh linens, large closet, large dresser and a full bathroom with tub and shower furnished with shampoo and soaps. A privacy door in the hallway separates the two bedrooms and baths.
PREMIUM AMENITIES INCLUDE: In unit Secure Wi-Fi Internet. High Definition 40', 1080P, television. Blue Ray DVD player with large selcection of movies and an internet connection to enjoy your favorite movie subscriptions. Beach Gear includes: snorkel masks and fins, coolers, beach chairs, boogie boards, tennis rackets, beach towels.
Ceiling fans in all rooms for year round comfortable breezes.
Enjoy your large covered wrap around lanai with ample patio furniture to sit and enjoy the views over the golf course, gently swaying palm trees and listen to the sound of the pounding surf . An additional dining table on the lanai invites that early morning cup of fresh Kona coffee while you listen to the birds singing.
Your fully equipped unit includes washer/dryer, fresh linens, an entertainment unit that includes a modern HDTV and blue ray DVD/CD player with a selection of movies and CD's for your enjoyment. Secure Wi-FI. Beach towels/snorkel gear/tennis racquets/coolers also in unit for your enjoyment. A previous guest made this comment in her review, 'The condo is fully equipped with everything: boogie boards, snorkel gear, tennis equipment, coolers, beach chairs, beach towels and more. The kitchen is a dream with a great selection of cooking utensils. Since our stay is an extended one, it is important to us to have all the comforts of home, and this is it. ' Both bathrooms have hair dryer and curling irons. Updated kitchen with newer stainless cook wear, granite counter tops and nice appliances. Complete supply of kitchen utensils including full set of designer dishes and baking cook wear. Extras include a full set of spices. One of our guests left this comment in her review, 'The well-stocked kitchen had everything we needed to make Christmas dinner, including nice pots, pans, mixing bowls, kitchen utensils, spices, oil, etc. '
This ocean front gated complex has 17 acres of lush, tropical landscaping with palm and coconut trees. Lot's of open space in our complex that is built around ancient Hawaiian ruins. Three freshly resurfaced, lighted tennis courts, a large pool with a shallow wading area fit for small children and a deeper end for swimming laps. Large covered pavillion with wi-fi and several tables and chairs for relaxing and socializing, plus a small room with library, soda machine and restrooms. Enjoy a covered barbeque and dining area for your outdoor cookouts and socializing. From your condo enjoy a short stroll to a secluded beach on the northern boundry of our complex with tidal pools, sea turtles and unique ses shells. In front of the complex is a paved walking path along the ocean with a picnic table for your relaxation and enjoyment of beautiful sunsets and whale watching. Take an evening stroll down the 13th fairway to the ocean with magnificant blow holes and sunsets. Bring your bottle of wine and meet the locals here.
A convenient and easy 8 minute walk to the famous Kahaluu beach for some great snorkling with the sea turtles around coral reefs and surfing. Only 3 blocks to the Keauhou Shopping Center that has groceries, drug store, many resturants and bars, shopping boutiques and a medical center plus more.
We know that you will love your stay in our home!
State of Hawaii Required Note: Our local property manager is PAEC Company and our Hawaii Tax ID is W26475210-01
Keywords: Condominium, Ocean Front Complex, Pool, Tennis Courts, Golf course views
My husband and I were both hard working professional business people, now retired, who need our vacation time to be relaxing, yet close to activities. We both grew up in midwest farm families and now live on the south coast of Oregon.. This condo is our second home and we spend as much time as possible here.
Maggie and Steve (owners) purchased this condo in 2012
We have traveled all over the world and this is our dream location to spend our free time with so many of the things we enjoy close by. Ocean sunsets, whale watching, snorkeling, scuba diving, surfing, golfing are all very close by. Very close to shopping and resturants. We love the friendly staff and beautiful landscaping with all the singing birds and open space of the Surf and Racquet Club complex.
We love to watch the whales and awesome sunsets just steps from our condo. Our quiet location in the complex makes for a relaxing snooze on our lanai. My hushand is an avid golfer and loves to play the course next door and the view of the 13th fairway from our lanai. I love to swim in the pool and listen to the sound of the surf from our lanai. We both love to snorkel and surf within a few minutes walk from our condo.
This review was submitted from our guestbook:
We enjoyed our stay in Kona! The weather was perfect, and this location was just what we had hoped for. We snorkeled and boogie boarded and fishing the blow hole at sunset was memorable. Thank you for providing a comfortable place to stay.
We truely felt at home.
We enjoyed our December winter vacation at this lovely Keahou Condo. It is nicely decorated, well furnished. It has a lovely wrap around lanai. We definitely will return.
We enjoyed staying at this condo for one week in November. For our purposes, it was perfect. The resort was quiet, relaxing, beautifully landscaped with tennis courts, a refreshing pool and close to some great snorkeling and a sandy beach. For meals, there were restaurants and a grocery store nearby. The deck overlooked a golf course where you could go for walks to watch the sunset. We had one small issue which Maggie and Steve resolved immediately. We would come back here in a heartbeat and would certainly recommend staying here if you are visiting Kona.
Thank you so much for letting us stay at your place. The condo had absolutely everything that we could have
possibly needed---------all the beach toys, coolers etc. The corner unit was fantastic with the large lani wrapping around two sides. It was so open and bright. The beds were very comfortable. You also took great care in your decor-----------loved it all. The view was very nice and quite private. Location was great. We did a hike near near the Kilauea Visitor Center down through the rain forest and across an old lava crater which was great. The Botanical Gardens in Hilo should not be missed. Did a dinner cruise with Body Glove which was excellent. We drove all over the island-----so much to see. Seven days was not enough time so
will simply have to go back! :)
We really enjoyed the 2 weeks we spent in this condo. It is perfectly located, very well equipped, clean, the lanai is beautiful, we really felt at home. Besides, Maggie and Steve gave us lots of information about nice places to vsit, they were really helpfull.
The swimming-pool is also very nice and the shopping center is near !
I hope we get the opportunity to come once again.
Mahalo nui loa, Steve & Margaret, for sharing & trusting your beautiful condo to us from May 12 to May 16, 2014. We had a very enjoyable & comfortable stay in your condo – everything we needed during our vacation was there & you provided them with the guests' needs in mind. The condo was very welcoming, clean, breezy & every room has its own ceiling fan & screened windows where you can open for the breeze to come in…a tropically nice feeling! All rooms were tastefully decorated with Hawaiian décor (artwork, furnitures, beddings, etc.) which I love so much & added to that tropical feeling!! When I was in the process of choosing a Kona condo, you were so patient & answered all our questions immediately. Even when we were already in the condo, you were always available. You even gave us suggestions on where to eat, where to watch the sunset, etc. If we have another chance to go back to Kona, I would not hesitate to stay in your condo & I would definitely refer your condo to our family & friends who might want to go to Kona for a relaxing vacation. Again, alooooha & mahalo so much to both of you.
Steve and Maggie are great rental owners! The condo is beautiful with great views. Complex is very nice, safe, and clean. Comfy beds and lots of clean linens, fully stocked kitchen, snorkel and beach gear, and entertainment systems. We stayed for 10 days and loved the condo. Highly recommended!
Next to Kona Country Club with a public 18 hole ocean front course and an 18 hole mountain course. 8 minute walk to great snorkel and surfing at Kahaluu Beach. 3 blocks to Keauhou Shopping Center with great resturants, bars, grocery store, Longs Drug store, movie theatre, post office, art galleries and boutiques. On bus route to get anywhere around Kailua Kona.
|Rate Period
|Weekend Night
|Monthly *
Apr 1 2015 - Jun 1 2015
4 night minimum stay
|Notes: Rates are based on 2 guests; add $5 per night, per additional guest
Jun 2 2015 - Oct 2 2015
4 night minimum stay
|Notes: Rates are based on 2 guests; add $5 per night, per additional guest
Oct 3 2015 - Oct 12 2015
7 night minimum stay
Oct 13 2015 - Dec 1 2015
3 night minimum stay
|Notes: Rates are based on 2 guests; add $5 per night, per additional guest
Dec 2 2015 - Dec 19 2015
6 night minimum stay
|Notes: Rates are based on 2 guests; add $5 per night, per additional guest
Dec 20 2015 - Dec 27 2015
6 night minimum stay
New Years
Dec 28 2015 - Jan 4 2016
6 night minimum stay
Winter 2016
Jan 5 2016 - Apr 16 2016
14 night minimum stay
|Notes: Rates are based on 2 guests; add $5 per night, per additional guest
My Standard Rate
4 night minimum stay
|Notes: Rates are based on 2 guests; add $5 per night, per additional guest
|Property Damage Protection
|Cleaning Fee
|Tax Rate
Rates are for 2 people. $5 per day each additional. Maximum 5 guests.
60 day cancellation policy.
Discounts for multi month reservations.
50% deposit due at booking, balance due 60 days prior to arrival.
Local management company available for maintenance or cleaning needs.
Hawaii Tax ID: W2647521001
* Approximate monthly rate. Actual rate will depend on the days of the month you stay.
Payment is usually accepted in the quoted currency (USD) unless the currency and the amount is specifically agreed in advance with the owner / advertiser.


text: Idea Exchange: Heating pads keep fluids toasty Mar 01, 2011 By dvm360.com staff VETERINARY MEDICINE To have warm fluids available when we need them, we lay a couple of unopened fluid bags on a heating pad set on low and then place another heating pad on top. This way warm fluids are always on hand but with less muss and fuss than with other methods. If we don't use them, it's no big deal. Dr. Lee Roberts Greencastle, Ind. Related linksRead more Idea Exchange tipsIdea Exchange: Pipe insulation keeps fluids flowing Hot topics on dvm360Pol on defense as Michigan veterinary board discusses negligence charges Photo gallery: The top 10 veterinary schools in America, according to U.S. News Front Desk Disasters, Episode 3: Dude looks like a lady Video: Flea hideouts in the house Veterinarians: Your clients are going to Google with these cat questions


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All eyes of the collector car world are focused on California this week as Monterey Week 2012 gets underway. Each year thousands of collector car enthusiast head west to participate in the weeks' activities which culminate at the esteemed Pebble Beach Concours d'Elegance. Included in the car shows, race car reunions, and road rallies are 5 high-end collector car auctions. Mecum, RM, Russo and Steele, Gooding and Company, and Bonhams all are holding events this week. Our handy guide to those Monterey auctions awaits you after the jump.
Three low-mileage C1 Corvettes are going under the microscope to help the National Corvette Restorers Society (NCRS) update its Technical Information Manual & Judging Guide for 1953-55 Corvettes.
A long-time Corvette restorer and NCRS member, Troy Pyles, volunteered for the task of examining the three cars, with the goal of improving and ensuring the accuracy of the current handbook and adding any newly found details. He talked first with David Brigham, the 1953-55 Corvette Judging Leader, to seek out all the knowledge he has collected through the years, before heading on to Effingham, Ill.
Billed as the world's largest one-day automotive event, the annual Woodward Dream Cruise in Detroit this Saturday is expected to attract more than a million car fanatics. Chevrolet is once again the presenting sponsor and one of the highlights will be the celebration of Corvette's 60th anniversary.
Classic Wisconsin Circuit Is Corvette's Proving Ground and Ancestral Home
ELKHART LAKE, Wis., Aug. 13, 2012 – The long and winding road to the American Le Mans Series championship runs through Road America, a track that is revered and respected by generations of sports car racers. The classic 4-mile circuit in the rolling Wisconsin countryside will host the Road America Road Race Showcase, the seventh round of the 2012 ALMS on Saturday, followed by the Corvette World Tribute on Sunday.
Racing legend Bob Bondurant gets a sneak peek, in this minute-long video from the Austin American-Statesman, at the Circuit of the Americas track under construction near Austin.
"Look at the elevation changes right now," Bondurant comments as he makes a reconnaissance lap of the 3.4-mile circuit track. "This is gonna be so cool."
Mecum Auctions will be in Monterey, California this weekend for their annual August sale. The auction runs August 16-18 at the Hyatt Regency Monterey Hotel and Spa on Del Monte Golf Course. Velocity will have 18 hours of live coverage of the auction. Nearly 60 Corvettes are scheduled to cross the block including a black 1968 L88 roadster, Mickey Thompson's 1963 Z06, and a COPO 1967 convertible shown at the NY Auto Show.
We first told you about the Corvette-based SV 9 Competizione back in the summer of 2009. At that time there were plans to build as many as 1,000 of the custom coaches for just under $100,000 apiece. That was before SV Motors' boss was indicted on investment fraud charges. It appears that just 4 of the carbon fiber-bodied cars were ever built. You've got your chance to score one member of that quartet at Mecum's Monterey auction next week in California.
If you live up in Northwest Washington State, be on the lookout for a Nassau Blue 1965 Corvette Coupe and an all-original 1970 Chevelle SS that were stolen from a warehouse in Puyallup, south of Seattle. The two classic cars were owned by Gary Tucci and were stolen sometime last Wednesday night.
Each year on the Wednesday before the annual Woodward Dream Cruise Corvette enthusiasts gather to support the Open Hands Food Pantry in Royal Oak, MI. Over the last few years organizer Larry Courtney has grown the event from a one-day cruise down famous Woodward Avenue to a multi-day festival of all things Corvette. This year's festivities kick off on Wednesday August 15th. Read on the see all of the activities on deck this year.
What would you like to see Tony Stewart drive during a special exhibition at Charlotte Motor Speedway on August 28?
It's up to the fans to make that choice as part of a special Mobil 1 Facebook page survey going on now through August 27.
Mobil 1 is sponsoring the voting that will decide the vehicle he'll be commandeering around the track: a Mobil 1 Racing-edition Bondurant Corvette Grand Sport, the Mobil 1 Formula Drift Chevy Camaro SS, a Chevy Astro Van, or a World War II-vintage tank.


text:
A guest post by Monica Shaw One of my big "achievements" of 2014 was #monthofbirthday. Most of us, including myself until last year, very humbly choose to celebrate our birthdays on one particular day, and if we're feeling extravagant, maybe for a weekend. But I decided that I would make July all about doing as much as […]
Paris, differently
Join me in Paris (NEW DATES: July 2015)
Who doesn't love Paris in the springtime (or anytime for that matter)? Well now you have the chance to join me in Paris, for seven days from March 8-15th 2015, to experience Paris differently – with the locals! EDITED ON January 17th 2015: We will now be offering this from July 19 – 26 2015! […]
A food tour by the Canal St Martin (Paris) with Localers
Many regular readers will know I love taking food tours wherever I go and that food tours in Paris are amongst my favourites! Last summer, I was excited to check out a couple of newcomers to the Paris walking tour scene – like Localers, whose Paris tour offerings are so varied you'll want to take […]
Learning to make mille feuille at La Cuisine Paris
It started with butter. A lot of butter… After a few summers of taking classes at La Cuisine Paris, I'm never surprised to see how much butter is used in their baking classes. Because all great pastries begin with butter, right? What is amusing, however, is watching other people's reactions to the amount of butter […]
A tour of the Marché d'Aligre (Paris) with Context Paris
Longtime readers won't be surprised to see another Paris food tour here on the blog – many of you know food tours are something I actively seek out wherever I go. I'm a huge fan of Context tours because they offer something a little different – claiming to be "committed to the character of the city", […]
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Searched for: "social chatter ios"
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Arts & Culture
2014 Denver Fall Theater Preview
There are lots of exciting new shows on the horizon this fall in Denver. Check out some of the newest plays and musicals to hit the local stage this season.
A Night At Denver's Edge Theater
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Quench your thirsty scientific brain with some hands-on play at any of these great science exhibits in Denver. Kids and adults alike will love exploring the many different concepts that each of these cover.
Top Upcoming Art Exhibits In Denver
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Top World Class Cultural Opportunities In Colorado's High Country
Experience some culture among the scenic beauty that is Colorado's High Country. Here are some of the best ways to check out the art this summer.
How To Get Involved In Denver's Theater Community
If you've always wondered how you can audition for shows or get involved in the local theater community, there are a few great resources you should know about.
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Get some clarity, relax and focus in on the things that matter most to you. Meditation can bring all of that and more. Here are a few of the best places to learn more about the art and practice of meditation in Denver.


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LOS ANGELES -- Occidental Petroleum CEO Stephen Chazen said low share prices mean the company will probably buy back shares and will take a closer look at options for selling assets or parts of the company.
"With a lower stock price, things that might not have worked before might work now. That isn't any kind of forecast, it's just sort of a tautology" he said.
Mr. Chazen said share buybacks are "probably in our future."
In addition, putting the company's midstream assets into a Master Limited Partnership (MLP) would be one option that would generate cash from the sale of the units, but one that would be "fine tuning rather than a major mover."
"You just look for things that at least initially are things that move the needle a lot rather than tweaking" he said.
Other possibilities mentioned during the conference call included selling assets and splitting off parts of the company.
"But I think you don't want to go down the path of sort of a delicatessen approach to this, where you slice a piece of baloney off and you throw it to the wolves" he said.
But on the other end of the spectrum, he said selling off the whole company is an "improbable outcome."
The company reported that profits fell by 13% to $1.36 billion. Occidental's board said earlier this year that it is looking for a new CEO. Mr. Chazen said that he would stay on through the search but would not comment beyond that.
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apparently wall street crooks already knew back in Jan.
don't get caught holding short
i agree
some of the tools here were shouting fire here last month. since then EXXI is up 40%. man these tools lost big time shorting oil stocks and this is nothing yet. just wait until summer, oil stocks will be trading double from these levels today. EXXI is easy 10.
I am so glad to know that you are so proud.
more to come.
Bought 5K last month at around 6. Already up almost 40%. Then I received an email from the broker, today received $2500 dividend. Go LRE. Oil has been going up slowly. By summer, LRE will be trading above 12. Lots of shorts to cover oil stocks.
Those hawkish talks about oil going to 30 is purely a mind game and not reality. Load up oil stocks. Gas at local stations have gone up 40 cents a gallon last week (in the 30 sq. mile area I checked). Smart people know oil cannot stay this low for more than a few months.
it is 20 now. going up every day.
load up
Nice job. You may cover at 70 by the end of this year. Everybody knows that this dive in oil prices is a political scam. Oil will be manipulated above 100 in 2015 itself.
Vnr and other oil stocks may double from here. heavy short covering ahead
Best start of year 2015. So 120000 x 0.5 = ?
Can you call brokers from mental facilities?
With connected cars and homes going to be a hot commodity and NXPi being in the early lead in these segments, do you guys see NXPI goes to 200-300 range in the next 5 years or so?


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HOOFPRINTS: Glow run upcoming
March 27, 2015 Hello Mustang Fans! The next event on tap for the Mustang Booster Club is the 5th Annual Mustang Stampede "Glow Run" and after party will be held on Friday, April 24, 2015, at the Schwan Regional... more »»
HOOFPRINTS: Winter tourneys, spring sports ahead
February 26, 2015 Hello Mustang Fans! It is a championship week for our winter sports as the SMSU women's and men's Basketball teams began the first round of the Northern Sun Intercollegiate Conference/Sanford Health... more »»
HOOFPRINTS: Hoofprints: Additional meaning for this weekend's games
February 19, 2015 Hello Mustang Fans! The SMSU Department of Athletics will be hosting "It's a Slam Dunk – Don't Drive Drunk" this Friday and Saturday, when the Mustangs play their final regular season home games of... more »»
HOOFPRINTS: Another successful Gold Rush Raffle at SMSU
February 12, 2015 MARSHALL — The Gold Rush Raffle was a huge success again this year with the SMSU Conference Center Ballroom packed with supporters of SMSU Athletics and the Mustang Booster Club. more »»
HOOFPRINTS: Hmielewski talks Hall of Honor, Hawaiian Night, Gold Rush Raffle
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HOOFPRINTS: Hall of Honor, Gold Rush Ahead
January 8, 2015 Hello Mustang Fans! Next week, the spring semester begins at SMSU. We have a busy week as many of our winter sports are competing on the road. more »»
HOOFPRINTS:Fall sports giving way to winter sports at SMSU
November 13, 2014 Winter is upon us. The fall sports regular season will come to a close this weekend with Mustang football's final game, and two SMSU teams are in postseason competition. more »»
Hoofprints: Hmielewski highlights Celebration of Excellence
October 16, 2014 Hello Mustang Fans! It was an amazing game-day environment on the campus of SMSU for Homecoming 2014 last Saturday. more »»
Hoofprints: Hmielewski outlines homecoming activities
October 10, 2014 Hello Mustang Fans! It is Homecoming Week at SMSU! This year's Homecoming theme is "Rise of the Mustangs", and we still have a host of events you and your family can attend throughout the week. more »»
Hoofprints: SMSU homecoming lies ahead
October 1, 2014 Hello Mustang Fans! Last Saturday, the SMSU Department of Athletics hosted the 8th Annual Ag Bowl presented by Ralco, Inc. more »»
Hoofprints: SMSU homecoming lies ahead
October 1, 2014 Hello Mustang Fans! Last Saturday, the SMSU Department of Athletics hosted the 8th Annual Ag Bowl presented by Ralco, Inc. more »»
Hoofprints: Ag Bowl activities ahead this weekend
September 25, 2014 MARSHALL—Hello Mustang Fans! This Saturday, the SMSU Department of Athletics will host the 8th Annual Ag Bowl presented by Ralco, Inc. The festivities will begin at 2:30 p.m. more »»
September 11, 2014 Hello Mustang Fans! Last Saturday was a beautiful day to host the 9th Annual Mustang Shootout at Shooter's Sporting Clays presented by the Marshall Radio Station and Shooter's Sporting Clay. more »»
MUSTANG HOOFPRINTS: Mustang Booster Club Drive underway
May 23, 2014 Hello Mustang fans! On Tuesday night, the Mustang Booster Club (MBC) hosted a Membership Drive Social at the Schwan Regional Event Center where new and current members gathered information and sign... more »»
MUSTANG HOOFPRINTS: Finals approaching; seasons ending
May 1, 2014 Hello Mustang Fans! Finals week at Southwest Minnesota State is next week. The SMSU softball and women's golf teams concluded their seasons last weekend. more »»
MUSTANG HOOFPRINTS: Spring season coming to fast conclusion
April 24, 2014 Hello Mustang Fans! The spring semester is winding down, and we are two weeks away from finals on the campus of Southwest Minnesota State University. more »»
MUSTANG HOOFPRINTS: Track and Field Complex taking shape
April 17, 2014 Hello Mustang Fans! On Monday, Southwest Minnesota State University and Marshall Public Schools broke ground on the Marshall Regional Track and Field Comple. more »»
MUSTANG HOOFPRINTS: Mustang app available now to be downloaded
April 3, 2014 Hello Mustang Fans! This week, the Southwest Minnesota State University Department of Athletics and sponsors, Aramark, Hy-Vee and Pepsi, are excited to announce a new way for fans to interact with... more »»
MUSTANG HOOFPRINTS: SMSU secures most pledges
March 27, 2014 Hello Mustang Fans! The NCAA, along with the T.E.A.M. more »»
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A method of interrogating an addressable array having a plurality of different chemical features. The method may include simultaneously illuminating different regions of the array with light of different intensities (which, for example, can, but need not be, also of a same waveband). Light emitted from the different regions in response to the illuminating (which light, for example, can, but need not be, of a same waveband) may be simultaneously detected with different detectors. An apparatus, addressable array unit, and related methods are further provided.
1-28. (canceled)
29. A method of interrogating an addressable array having a plurality of different chemical features, the method comprising:
(a) simultaneously illuminating different regions of the array with light of different intensities and a ratio of intensities of at least 5/1; and
(b) simultaneously detecting light emitted from the different regions in response to the illuminating.
30. A method of interrogating an addressable array having a plurality of different chemical features, the method comprising:
(a) simultaneously illuminating different regions of the array with light of different intensities and of the same waveband; and
(b) simultaneously detecting light emitted from the different regions in response to the illuminating;
wherein the different regions are simultaneously illuminated with light of different intensities and of a same waveband.
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50. An apparatus for interrogating an addressable array having a plurality of different chemical features, the apparatus comprising:
(a) a light system which simultaneously illuminates different regions of the array with light of different intensities and a ratio of intensities of at least 5/1; and
(b) light detectors which simultaneously detect light emitted from the different regions in response to the illuminating by the light system.
51. An apparatus for interrogating an addressable array having a plurality of different chemical features, the apparatus comprising:
(a) a light system which simultaneously illuminates different regions of the array with light of different intensities and of the same waveband; and
(b) light detectors which simultaneously detect light emitted from the different regions in response to the illuminating by the light system.
52. The apparatus according to
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This application claims priority to copending application Ser. No. 10/261,807, filed Sep. 30, 2002, under 35 U.S.C. 120, the entirety of which is incorporated herein by reference.
This invention relates to arrays, particularly biopolymer arrays such as DNA or protein arrays, which are useful in diagnostic, screening, gene expression analysis, and other applications.
For the purposes of the discussion below, cited art is not admitted to constitute prior art to the present application.
Polynucleotide arrays (such as DNA or RNA arrays) and peptide arrays, are known and may be used, for example, as diagnostic or screening tools. Such arrays include regions (sometimes referenced as spots or features) of usually different sequence polynucleotides or peptides arranged in a predetermined configuration on a substrate. The array is �addressable� in that different features have different predetermined locations (�addresses�) on a substrate carrying the array.
Biopolymer arrays can be fabricated using in situ synthesis methods or deposition of the previously obtained biopolymers. The in situ fabrication methods include those described in U.S. Pat. No. 5,449,754 for synthesizing peptide arrays, and in U.S. Pat. No. 6,180,351 and WO 98/41531 and the references cited therein for polynucleotides. In situ methods also include photolithographic techniques such as described, for example, in WO 91/07087, WO 92/10587, WO 92/10588, and U.S. Pat. No. 5,143,854. The deposition methods basically involve depositing biopolymers at predetermined locations on a substrate which are suitably activated such that the biopolymers can link thereto. Biopolymers of different sequence may be deposited at different feature locations on the substrate to yield the completed array. Procedures known in the art for deposition of biopolymers, particularly DNA such as whole oligomers or cDNA, are described, for example, in U.S. Pat. No. 5,807,522 (touching drop dispensers to a substrate), and in PCT publications WO 95/25116 and WO 98/41531, and elsewhere (use of a pulse jet in the form of a piezoelectric inkjet head).
Further details of large scale fabrication of biopolymer arrays by depositing either previously obtained biopolymers or by the in situ method, are disclosed in U.S. Pat. No. 6,242,266, U.S. Pat. No. 6,232,072, U.S. Pat. No. 6,180,351, and U.S. Pat. No. 6,171,797.
In array fabrication, the quantities of DNA available for the array are usually very small and expensive. Sample quantities available for testing are usually also very small and it is therefore desirable to simultaneously test the same sample against a large number of different probes on an array. These conditions require the manufacture and use of arrays with large numbers of very small, closely spaced features.
The arrays, when exposed to a sample, will exhibit a binding pattern. The array can be read by observing this binding pattern by, for example, labeling all targets such as polynucleotide targets (for example, DNA), in the sample with a suitable label (such as a fluorescent compound), scanning an illuminating beam across the array and accurately detecting the fluorescent signal from the different features of the array. Assuming that the different sequence polynucleotides were correctly deposited in accordance with the predetermined configuration, then the observed binding pattern will be indicative of the presence and/or concentration of one or more polynucleotide components in the sample. Peptide or arrays of other chemical moieties can be used in a similar manner.
Techniques and apparatus for scanning chemical arrays are described, for example, in U.S. Pat. No. 6,406,849, U.S. Pat. No. 6,371,370, U.S. Pat. No. 6,355,921, U.S. Pat. No. 5,763,870 and U.S. Pat. No. 5,945,679. Apparatus which reads an array by scanning an illuminating beam by the foregoing technique are often referred to as scanners and the technique itself often referred to as scanning. Conventionally, such scanning has been done by illuminating array features on a front surface of the substrate one pixel at a time.
Array scanners typically use one or more laser beams of different waveband as light sources, which are scanned over pixels covering the array features. The lasers are generally set to provide as much light as possible to a scanned array, and consequently the relative intensities of such different waveband light sources are close to equal, or about 2/1 or less. detectors (typically fluorescence detectors) each with a very high light sensitivity is normally desirable to achieve maximum signal-to-noise in detecting hybridized molecules, particularly in array scanners used for DNA sequencing or gene expression studies. At present, photomultiplier tubes (�PMTs�) are still the detector of choice although charge coupled devices (�CCDs�) and avalanche photodiodes (�APDs�) can also be used. PMTs and APDs are typically used for temporally sequential scanning of array features, while CCDs permit scanning many features in parallel (for example, one line of features simultaneously, in which case an illuminating line may be used).
A difficulty in reading chemical arrays is that given the large numbers of features which may be present, for example, many thousands of features, and given the various samples that may be exposed to the array, the fluorescent signals detected from different features can vary over a wide range of intensities. In order to obtain the maximum signal from a feature it is desirable to illuminate the array features with light of an intensity and for a time, as high as possible so as to obtain a detectable signal even from features which may only have a few labels present. On the other hand though, in this situation a detected signal from a feature having many labels may be so strong as to cause detector saturation (that is, further increases in signal from the array feature no longer cause an increase in detector signal output). In this event, meaningful measurements from such features are lost. The range over which meaningful signals may be obtained from a feature by an array reader may be referenced as its dynamic range.
It would be desirable then, to provide an array reader with a high dynamic range covering both features which may produce a low or high detectable signal, and which is not overly complex to construct.
The present invention provides a method of interrogating an addressable array having a plurality of different chemical features. The method may include simultaneously illuminating different regions of the array with light of different intensities (which, for example can, but need not be, of a same waveband). Light emitted from the different regions in response to the illuminating (which light can, but need not be, of a same waveband) may be simultaneously detected with different detectors.
The present invention further provides an apparatus which includes a light system to provide the simultaneous illumination to the different regions of the array, and different light detectors for the simultaneous detection from the different regions, both as mentioned above.
The present invention further provides an addressable array unit. The addressable array unit may have a plurality of different chemical features (such as different polynucleotide or poly-amino acid features). The array unit may also include instructions that different regions of the array can be simultaneously illuminated with light of different intensities and that light emitted from the different regions in response to the illuminating can be simultaneously detected from those regions with different detectors. The instructions may further include an indication of the relative light intensities for the simultaneously illuminated regions or detector sensitivities for the simultaneously detected regions. Alternatively, rather than the array unit including any instructions, it may instead include a code (for example, a machine readable code) linked to such instructions or a combination of part of the instructions and a code linked to the remainder. Such a code can, for example, be located on a substrate carrying the array or an element which carries the substrate.
The present invention also provides a method for receiving a code as already described, and communicating the instructions in response to the received code.
The present invention may provide one or more of the following or other benefits. For example, a high dynamic range may be obtained without a need for scanning the array multiple times.
Embodiments of the invention will now be described with reference to the drawings, in which:
To facilitate understanding, the same reference numerals have been used, where practical, to designate similar elements that are common to the FIGS.
In the present application, unless a contrary intention appears, the following terms refer to the indicated characteristics. A �biopolymer� is a polymer of one or more types of repeating units. Biopolymers are typically found in biological systems and particularly include polysaccharides (such as carbohydrates), and peptides (which term is used to include polypeptides and proteins) and polynucleotides as well as their analogs such as those compounds composed of or containing amino acid analogs or non-amino acid groups, or nucleotide analogs or non-nucleotide groups. This includes polynucleotides in which the conventional backbone has been replaced with a non-naturally occurring or synthetic backbone, and nucleic acids (or synthetic or naturally occurring analogs) in which one or more of the conventional bases has been replaced with a group (natural or synthetic) capable of participating in Watson-Crick type hydrogen bonding interactions. Polynucleotides include single or multiple stranded configurations, where one or more of the strands may or may not be completely aligned with another. A �nucleotide� refers to a sub-unit of a nucleic acid and has a phosphate group, a 5 carbon sugar and a nitrogen containing base, as well as functional analogs (whether synthetic or naturally occurring) of such sub-units which in the polymer form (as a polynucleotide) can hybridize with naturally occurring polynucleotides in a sequence specific manner analogous to that of two naturally occurring polynucleotides. For example, a �biopolymer� includes DNA (including cDNA), RNA, oligonucleotides, and PNA and other polynucleotides as described in U.S. Pat. No. 5,948,902 and references cited therein (all of which are incorporated herein by reference), regardless of the source. An �oligonucleotide� generally refers to a nucleotide multimer of about 10 to 100 nucleotides in length, while a �polynucleotide� includes a nucleotide multimer having any number of nucleotides. A �biomonomer� references a single unit, which can be linked with the same or other biomonomers to form a biopolymer (for example, a single amino acid or nucleotide with two linking groups one or both of which may have removable protecting groups). A biomonomer fluid or biopolymer fluid reference a liquid containing either a biomonomer or biopolymer, respectively (typically in solution).
An �array�, unless a contrary intention appears, includes any one-, two- or three-dimensional arrangement of addressable regions bearing a particular chemical moiety or moieties (for example, biopolymers such as polynucleotide sequences) associated with those regions. An array is �addressable� in that it has multiple regions of different moieties (for example, different polynucleotide sequences) such that a region (also referenced as a �feature� or �spot� of the array) at a particular predetermined location (an �address�) on the array will detect a particular target or class of targets (although a feature may incidentally detect non-targets of that feature). Note that the finite small areas on the array which can be illuminated and from which any resulting emitted light can be simultaneously (or shortly thereafter) detected, define pixels which are typically substantially smaller than a feature (typically having an area about {fraction (1/10)} to {fraction (1/100)} the area of a feature). Array features are typically, but need not be, separated by intervening spaces. In the case of an array, the �target� will be referenced as a moiety in a mobile phase (typically fluid), to be detected by probes (�target probes�) which are bound to the substrate at the various features. However, either of the �target� or �target probes� may be the one which is to be evaluated by the other (thus, either one could be an unknown mixture of polynucleotides to be evaluated by binding with the other). An �array layout� refers to one or more characteristics of the features, such as feature positioning on the substrate, one or more feature dimensions, and an indication of a moiety at a given location. The array �substrate� includes everything of the array unit behind the substrate front surface. �Hybridizing� and �binding�, with respect to polynucleotides, are used interchangeably.
When one item is indicated as being �remote� from another, this is referenced that the two items are at least in different buildings, and may be at least one mile, ten miles, or at least one hundred miles apart. �Communicating� information references transmitting the data representing that information as electrical signals over a suitable communication channel (for example, a private or public network). �Forwarding� an item refers to any means of getting that item from one location to the next, whether by physically transporting that item or otherwise (where that is possible) and includes, at least in the case of data, physically transporting a medium carrying the data or communicating the data. An array �unit� may be the array plus only a substrate on which the array is deposited, although the unit may include other features (such as a housing with a chamber). A �chamber� references an enclosed volume (although a chamber may be accessible through one or more ports). It will also be appreciated that throughout the present application, that words such as �top�, �upper�, and �lower� are used in a relative sense only. Wavelengths are peak wavelengths unless otherwise indicated.
A �same waveband� in relation to the light illuminating the array means having a spectral peak at the same wavelength, plus or minus 20 nm (and can include the same plus or minus 10, 5 or 1 nm). In an array reader which reads the array in different wavebands (for example, different color channels) any band overlap is best minimized by using light sources with narrow half-height peak emission spectra (for example, no more than 20, 10, or 5 nm) such as provided by different color lasers of half-height emission width typically smaller than 0.1 nm. However, for or emission of fluorescent dyes or phosphors the half-height width may be wider. Wavelengths are measured in a gas (such as air or nitrogen), or a vacuum, whichever one is in contact with the array during reading, unless the contrary is indicated.
By light �intensity� is referenced photon flux per unit area (that is, the number of photons per unit area per unit time, such as photons/second/m2).
A �processor� references any hardware and/or software combination which will perform the functions required of it. For example, any processor herein may be a programmable digital microprocessor such as available in the form of a mainframe, server, or personal computer (desktop or portable). Where the processor is programmable, suitable programming can be communicated from a remote location to the processor, or previously saved in a computer program product (such as a portable or fixed computer readable storage medium, whether magnetic, optical or solid state device based). For example, a magnetic or optical disk may carry the programming, and can be read by a suitable disk reader communicating with each processor at its corresponding station. Reference to a singular item, includes the possibility that there are plural of the same items present. �May� means optionally. Methods recited herein may be carried out in any order of the recited events which is logically possible, as well as the recited order of events. All patents and other references cited in this application, are incorporated into this application by reference except insofar as they may conflict with those of the present application (in which case the present application prevails).
Referring now to
The probes of features 16 are typically linked to substrate 10 through a suitable linker, not shown. Suitable materials for base substrate 10 include, for example, silica, glass, sapphire, or a plastic (that is, a synthetic or processed high molecular weight polymer that is, or is not, thermosetting or thermoplastic). Substrate 10 may have a thickness of at least 1 micrometer (or at least 5, 10 or 100 micrometers), or at least 1 mm. Substrate 10 may be transparent to the wavelength of the interrogating and detected emitted light. The front surface 11 a of substrate 10 may be modified with one or more different layers of compounds that serve to modify the properties of the surface in a desirable manner. Front surface 11 a should ideally exhibit a low level of binding during hybridization or other events.
The array 12 may cover an area of less than 100 cm2, or even less than 50, 10 or 1 cm2. In many embodiments, substrate 10 will be shaped generally as a rectangular solid (although other shapes are possible), having a length of more than 4 mm and less than 1 m, usually more than 4 mm and less than 600 mm, more usually less than 400 mm; a width of more than 4 mm and less than 1 m, usually less than 500 mm and more usually less than 400 mm; and a thickness of more than 0.01 mm and less than 5.0 mm, usually more than 0.1 mm and less than 2 mm and more usually more than 0.2 and less than 1 mm.
An array identifier 40 in the form of a machine readable bar code in
Arrays such as those of
In use, the features 16 of array 12 are simultaneously exposed to a sample such as by contacting the different features 16 simultaneously with a layer of the same sample fluid, such that at least some of the features bind to respective moieties in the sample which sample moieties include the fluorescent label. For example, different polynucleotide sequences at respective features 16 can each hybridize to a complementary sequence, such as polynucleotides at feature 16 a in
Referring now to
The two interrogating beams of the red pair originating from laser 100 a and beam splitter 122 a are directed along respective paths 130 a, 130 b, while the two interrogating beams of the green pair originating from laser 100 b and beam splitter 122 b are directed along respective paths 130 c, 130 d. Light is directed along all of the paths 130 a, 130 b, 130 c, 130 d by means of full mirror 151, dichroic mirror 153, and full mirror 156 onto four different locations of an array being read (namely an array 12 of an array unit 30 mounted on holder 200), using optical components in beam focuser 160. Note though that
Each of the four beams provided on paths 130 a, 130 b, 130 c, 130 d then provide four spatially separated spots on an array 12 of an array unit 30 mounted on holder 200. These may be focused on front surface 11 a directly without passing through substrate 10 when the array is being read with front surface 11 a facing beam focuser 160 (that is, facing down in
Light emitted, in particular fluorescence, at two different wavelengths (for example, green and red light) from regions illuminated by spots 140 a, 142 a, 140 b, 142 b, in response to the interrogating light, is imaged using the same optics in focuser/scanner 160, and is reflected off mirrors 156 and 154. The two different wavelengths are separated by a further dichroic mirror 158. There will be four paths of detection each separated by a small angle, resulting from the spaced interrogating light spots 140 a, 142 a, 140 b, 142 b. As already mentioned though, for the sake of clarity these are only shown as one path in
Instead of using dichroic 158, one can also use a design that images the different scanning spots onto different light-guiding fibers that then guide the signal from each one of these to a different detector. Such an arrangement for two scanning spots is described in U.S. Pat. No. 6,320,196.
The detectors 150 a, 152 a are set with the same sensitivities to one another, as are detectors 150 b, 152 b. However, some variation in sensitivities is acceptable although typically any sensitivity difference should be less than the excitation intensities, so that one obtains different �overall� sensitivities from the two detectors of a pair for the same waveband. In the foregoing, �overall sensitivity� is the product of illumination power and detector sensitivity. Similarly, detector 150 b which detects green fluorescent light from the region of more intensely illuminated spot 140 b will be set to the same or somewhat varied sensitivities as discussed above in connection with detectors 150 a, 152 a. This then allows for simultaneously detecting with the same or somewhat varied sensitivity detectors 150 a, 152 a, light emitted from the different regions 140 a, 142 a in response to the simultaneous illuminating with light of different intensities of the same waveband. This is similarly true for detectors 152 b, 150 b and spots 142 b, 140 b, respectively.
In order to raster scan spots 140 a, 142 a, and 140 b, 142 b in the manner already described, the scanner is provided with a scan system. In this manner, each of the multiple features 16 of the array is read, with each read feature containing multiple pixels (for example, more than five, ten, or twenty). This can be accomplished by providing a housing 164 containing mirror 158 and focuser 160, which housing 164 can be moved in a first direction along a line (that is, from left to right or the reverse as viewed in
An autofocus detector 170 is also provided to sense any offset between different locations on array 12 when in the reading position, and a determined position of the focal plane of the detection system. An autofocus system includes detector 170, processor 180, and a motorized adjuster to move holder in the direction of arrow 196. A suitable chemical array autofocus system is described in pending U.S. patent application Ser. No. 09/415,184 for �Apparatus And Method For Autofocus� by Dorsel et al., filed Oct. 7, 1999, incorporated herein by reference, as well as European publication EP 1091229 published Apr. 11, 2001 under the same title and inventors.
Controller 180 of the apparatus is connected to receive signals from the detector pair 150 a, 152 a (these signals being one �channel�), while also receiving signals from detector pair 150 b, 152 b (forming another �channel�). The signals in each channel are obtained at each of the two detected wavelengths from emitted light for each scanned pixel on array 12 when at the reading position mounted in holder 200. Controller 180 also receives the signal from autofocus offset detector 170, and provides the control signal to EOM 110, and controls the scan system. Controller 180 may also analyze, store, and/or output data relating to emitted signals received from detectors 150 a, 150 b in a known manner. The controller 180 may optionally set any one or more of the following: the light intensities for the simultaneously illuminated spots 140 a, 142 a (by controlling EOM 122 a); the light intensities of the simultaneously illuminated spots 140 b, 142 b (by controlling EOM 122 b); and the sensitivities of one or more of the four detectors. This setting can be made in response to instructions which may include an indication of the light intensities for the simultaneously illuminated regions of a same waveband or detector sensitivities for the simultaneously detected regions. Such instructions may be carried in identifier 40 read by bar code reader 168, or the read code may be linked to a file containing such instructions which are either located in a local or remote memory (for example, in a manner the same as which various items of array information can be obtained using a bar code, as described in U.S. Pat. No. 6,180,351). In the case of a code linked to a file such as may be located in a remote memory, the code may be received and the instructions linked to that code retrieved from memory based on the code and then communicated back to the location from which the code was received. Note that in the apparatus of
Controller 180 may include a computer in the form of a programmable digital processor, and include a media reader 182 which can read a portable removable media (such as a magnetic or optical disk), and a communication module 184 which can communicate over a communication channel (such as a network, for example the internet or a telephone network) with a remote site (such as a database at which information relating to array unit 30 may be stored in association with the identifier 40). Controller 180 is suitably programmed to execute all of the steps required by it during operation of the apparatus, as discussed further below. Alternatively, controller 180 may be any hardware or hardware/software combination which can execute those steps.
In one mode of operation, the array in unit 30 is typically first exposed to a liquid aqueous sample (for example, placed directly on substrate 10). The array may then be washed with buffer then water, and dried following washing then inserted into a scanner for reading. Drying may be accomplished using any suitable drying method and conditions which will not decompose the probes and their bound targets, such as any suitable one or more of: air drying at room temperature or raised temperature; reduced pressure; centrifuging; or exposure to a dry unreactive gas stream (such as dry nitrogen). Following a given array unit 30 being mounted in the apparatus, the identifier reader may automatically (or upon operator command) read array identifier 40, and use this to retrieve information on the array layout as well as any further instruction as to how the array should be interrogated or read (for example, the intensities of different interrogating lights or the sensitivities of different detectors, as described above). As already mentioned, such information may be retrieved directly from the contents of identifier 40 when identifier 40 contains such information, with the retrieved information being based wholly or only in part upon the read identifier 40. Alternatively, identifier 40 may be used to retrieve such information from a database containing the identifier in association with such information. Such a database may be a local database accessible by controller 180 such as may be contained in a portable storage medium in drive 182 which is associated with package 30, such as by physical association with package 30 when received by the user (for example, by being in the same package), or by a suitable identification. Alternatively, the database may be a remote database accessible by controller 180 through communication module 184 and a suitable communication channel (not shown). The retrieved instruction on how the array should be interrogated and read, can be used by controller 180 to control or check any one or more of: light intensity for each interrogating light spot on the array; detector sensitivity for each light spot region on the array; and oriented of the array within the reader (for example, if the array 12 is facing toward focuser 160 (when the array is to be illuminated and read without light passing through the substrate) or away from it (for illuminating and reading the array through the substrate). Assuming that lasers 100 a, 100 b are already operating, controller 180 then operates EOMs 110 a, 110 b and detectors 150 a, 152 a, 150 b, 152 b so that each detector generates a signal from a corresponding one of the spots as it is raster scanned over the array 12, in the manner already described. Signals read from the two detectors within a channel can be combined into one file for that channel by controller 180, for subsequent display or further processing.
In apparatus and methods of the present invention, use of two interrogated regions of the same waveband but different intensities to obtain a high total dynamic range, allows the use of simple optical elements to split an interrogating light source beam. This can be advantageous over illuminating only one region in each color channel and trying to split the detected emitted light in some proportion in each channels with a beam splitter. This is so since it is generally more difficult to construct an optical system which will split a beam to a desired ratio of intensities as the spectral width (for example, the width of the � maximum peak height) increases, and the emitted fluorescent light tends to have such a higher spectral width than an interrogating laser light. This difficulty can be further exacerbated by the fact that different end users might use different fluorescent labels in a same waveband, which different labels have different emission spectra. Thus, desired beam splitting ratios are easier to obtain for laser light than for the fluorescence the former excites.
The saved results from a sample exposed array, read according to a method of the present invention, may be raw results (such as fluorescence intensity readings for each feature in one or more color channels) or may be processed results such as obtained by rejecting a reading for a feature which is below a predetermined threshold and/or forming conclusions based on the pattern read from the array (such as whether or not a particular target sequence may have been present in the sample or whether an organism from which the sample was obtained exhibits a particular condition). The results of the reading (processed or not) may be forwarded (such as by communication of data representing the results) to a remote location if desired, and received there for further use (such as further processing).
It will be appreciated that various modifications can be made to the present invention. For example, a variety of geometries of the features 16 may be constructed other than the organized rows and columns of the array of
Other various modifications to the particular embodiments described above are, of course, possible. Accordingly, the present invention is not limited to the particular embodiments described in detail above.
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text: Though probably not as niche as Rural Free Delivery (RFD HD), World Fishing Network HD still has a pretty narrow audience. That being the case, there's still some logic to pushing the channel in rural parts of America or in regions with nearby fishing outlets. Call us crazy, but New York City is not the first place that comes to find when someone mentions deep sea fishin'. Nevertheless, Verizon has just announced that WFN HD has arrived in the Big Apple on FiOS TV, giving transplants an easy way to get back in touch with their small town roots. There's no word on when the station will hit other FiOS TV areas, but we'd bet it'll be making the rounds soon enough.


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Top 10 'Must-Have' Hostas
I'm fairly new to serious Hosta collecting, although I've been growing them on a lesser level for 20 years. So, I'm interested in hearing the "Top 10" lists from the old-hands here in the Hosta world, so I know which ones I should be looking for...
To start off, here's my OWN top 10, and keep in mind, these are the ones I currently HAVE, not the ones I WANT. In addition, these are all young plants, added to my collection within the last 3 years:
- ('Cause that's how Letterman does it)
Twist of Lime (for it's compact mini-size, brilliant color, and extremely fast growth)
9. Gold Standard (color, again, and size)
8. Fortunei Albopicta (for the markings and unique growth, but It grows SO slowly!)
2009 (same plant, no divisions)
7. Lakeside Little Gem (ADORABLE! And fast growing, and it gets these gorgeous degrees of green-to-blue hues)
6. Blue Cadet (one of the most gorgeous blues I've ever seen, neat medium size, and decent growth)
5. Golden Tiara (gorgeous color, great growth, a perfect focal point)
4. Regal Splendor (amazing color, interesting upright growth, great size)
3. Queen Josephine (slow growth & medium size allows it to be put in a focal point, beautiful patent leather sheen, lovely color)
2. Paul's Glory (show-stopper, large leaves, gorgeous texture & coloring)
1. Tokudama Flavocircinalis (coloring, size, texture (as plant matures))
Of course, this list will likely change tomorrow, and CERTAINLY will change by next year. I can't wait to see what you all have!
Ptilda, can't do it - got to top 20 but thats it, and only one is a 09 purchase (Popcorn) and one from 08 (Guardian Angel) - so gonna get even tougher next year as they mature.
Top Twenty Hosta (not in order)
Row 1 - Aristocrat, Blue Arrow, Dream Weaver, Fair Maiden
Row 2 - Frances Williams, Golden Prayers, Guardian Angel, Jimmy Crack Corn
Row 3 - June, Liberty, Love Pat, Montana Aureomarginata
Row 4 - Paradigm, Popcorn, Sagae, Striptease
Row 5 - Sun Power, Whirlwind, Wide Brim, Krossa Regal
I'm just a wimp to cut those last 10 - lol.
How cool! NONE of yours were mine... which means you obviously have a much more exquisite taste in Hosta! lol But I have both June and Striptease on their way to me (along with a dozen others), so I'm SUPER excited about that.
Can you give me an idea of why these are your favorites? I don't need all the dirty details, just an overview of what you look for in a "favorite".
My Top 20 tonight (subject to change tomorrow or whenever I see someone elses list) if you only want 10 - sort them in a random order. I am not cutting any of them off the list!
Blue Mouse Ears
Royal Standard
Regal Splendor
Stained Glass
Old Glory
El Capitan
Little Doll
El Nino/ First Frost (either one)
Olive Bailey Langdon
Savannah Supreme
Guardian Aangel
Rainforest Sunrise
Niagara Falls
I don't know about taste - unless you consider box wide exquisite - lol. Twist of Lime is on my short list for '10. I love Gold Standard, Regal Splendor, and Paul's Glory; my Toku Flavo is too young, but I know for some this is one of the most beautiful hosta.
Alright here goes for reasons-
Aristocrat - dusty grey blue with nice margin as matures - some struggle with this one
Blue Arrow - Great leaf and mound shape, holds blue for me, good grower
Dream Weaver - Not great grower for me, but great leaves - think more sun would help - I think DonR and Papou have huge specimens
Fair Maiden - Variety of Colors is beautiful and not common - can be finicky, a bit thin so can look worn later in season
Frances Williams - Great Colors, Leaf Size, Presence - burns easily for many, but usually pretty clear of burn for me
Golden Prayers - like this more each year - getting pebbled look and holds color pretty well, smaller
Guardian Angel - whats not to like - growing well, colors, texture
Jimmy Crack Corn - Deep veins, wavy, pointed, just have to look at this one when I walk by
June - Good grower, color, changes nice form, must have
Liberty - great spring color, must have
Love Pat - nice dusted blue that holds for me, good consistent cupping, growing well, wife's name
Montana Aureomarginata - the one I look for (notice) in every hosta garden - see a mature one and you'll know why
Paradigm - Texture, colors, changes color, handle sun, good grower for me
Popcorn - new for me in 09, love the small look, nice substance, great colors - hope it keeps current color even gets better
Sagae - must have, vase shape, margins cut into center color
Striptease - 1st one showed hvx when getting bigger - then bought nice sized end of season - this year, wow! Nice mound and color.
Sun Power - Made a jump over last two years - nice form, wavy edges, long pointed leaves, color
Whirlwind - pointed twisting, cream/misty green center, very different and unique
Wide Brim - as it is maturing with wider margins and textured leaves I just like it better each year.
Krossa Regal - tall, nice dusty blue early, Vase Shape. Another of the see a mature one and you'll want one.
I've added a lot of wavy/rippled edges so I know this list will change quite a bit as those mature.
These are my favorites this year. How about top 30? I cheated even more by listing families. Sorry, I couldn't go any lower.
June/ Touch of Class/ Ann Kulpa/ Halcyon
Blue Mouse Ears
Guacamole/ Tortilla Chip/ Stained Glass
Krossa Regal/ Regal Splendor/ Regal Promenade
Liberty/ Majesty/ Sagae
Old Glory
Brother Ronald
Silver Bay
Blue Jay
Bressingham Blue
El Nino/ First Frost
Olive Bailey Langdon
Earth Angel/Guardian Angel/ Blue Angel
Maui Buttercups/Rainforest Sunrise
Bright Lights/ Tok. Flavo.
Abiqua Recluse/ Paradigm (Slow for me but getting there)
Sum and Substance
Potomac Pride
Jade Cascade
Mount Everest (great grower this year)
Alex Summers
Alligator Shoes
Ventricosa Areomarginata
War Paint
Golden Meadows
Golden Waffles/ Golden Sculpture
Spilt Milk
Dream Queen/ Thunderbolt
Top 10 must haves: (only 10 ;( ! )
1. Kossa Regal
2. Regal Splendor
3. Sagae
4. Blue Cadet
5. Dream Weaver
6. Gold Standard
7. Sum & Sub
8. Gand Tiara (any Tiara)
9. Gaucamole
- Earth Angel (any large blue)
Just a list of the basic Hosta many of us had as a start to our obsession...
Your hosta labeled #8 albopicta is not labeled correctly.
It looks like Great Expectations to me. There are a few look alikes to GE, but definitely is not Albopicta.
I'm with Dave on this one, soon as I saw the picture and then the comment about its growth, GE has a reputation for being a slow grower, none of the Fortunei family are that I know of.
On to my top 10 list, in no particular order:
Blue Mouse Ears - I call it my desert island Hosta
Holy Mouse Ears - see a theme here....
Sea Octopus
Maui Buttercups
Fire Island
Monashee Blue Cup
Blue Moon
Dream Queen
Twisting The Night Away
I'll go hunt for some pix and will post those later...
Here are my top 12 must haves for any garden. Some are oldies but there are so many fabulous new ones that far surpass the oldies that I prefer them:
1. Sum and Substance
2. Blue Mammoth
3. Jewel of the Nile
4. Rainforest Sunrise
5. June
6. Queen of the Seas
7. Embroidery
8. Liberty
9. Grand Tiara
- Sea Fire
- Pineapple Upside Down Cake
- Fair Maiden
Bear in mind that I'm not crazy about greens with white or gold edges, unless they are magnificent. That's why Fair Maiden and Liberty make the list.
Here goes with my top ten [plus] list:
Montana Aureomarginata--outstanding vase shape, size, colors
Yellow River--same as above
Blue Cadet--a wonderful blue, neat size, grows fast, gorgeous blooms
June--great colors and shape--slug proof--very dependable
Striptease--wonderful colors--fast grower--nice sized
Shade Fanfare--I have never seen a more exciting green in a hosta
Dream Queen/Thunderboldt--visitors always ask what their names are--very striking in color , size, and form
Royal Standard: magnificent size, color, adaptability--gorgeous aromatic flowers
Patriot--every garden needs the pop that only a green and white Hosta offers
Liberty--the biggest "eye popper" of all
Sun Power--every garden also needs a large gorgeous dependable yellow
Spilt Milk--I love the uniqueness of the colors --and the name of this H
Now when is someone going to ask for a top 50 list--that would be so easy!!! LOL Mary
Hmmm, only 10?
Okay--my list will confirm so many others:
Regal Splendor
Krossa Regal
Jewel of the Nile
Summer Breeze
Cutting Edge
Sum and Substance
Blue Angel
Earth Angel
Guardian Angel
August Moons (many of them)
Jimmy Crack Corn
Dick Ward
Stain Glass
Fragrant Bouquet
Okay--that's 23--sorry--I don't think I can eliminate a one of them either!
Thanks for the shopping list guys ;)
LOL - my favs are different yet again (and in no order, order changes daily):
1. NOID (I know helpful right - but I posted a pic yesterday asking for the ID)
2. June
3. Night Before Christmas
4. Captain Kirk
5. Blue Mouse Ears
6. Patriot
7. Gold Standard
8. First Frost
9. Spilled Milk
- Paul's Glory
1) Empress Wu
2) Lemon Lime, Twist fo Lime, Lemon Frost
3) Pineapple Upsidedown Cake, Pineapple Punch, Pineapple Juice, Pineapple Poll
4) Dark Star
5) Ani Machi aka Geisha
6) Cherry Berry
7) All Mice
8) Blue Ivory
9) Tequila Sunrise
10)Samual Blue
- Clovelly
1. Blue Angel (IMO, the best big blue)
2. Sum and Substance (grows for me in all day sun)
3. Sagae (right at the entrance to my garden)
4. Liberty
5. montana Aureomarginata
6. June
7. Dancing Queen (right now it dominates my deck)
8. Lakeside Cha Cha
9. Regal Splendor
- Dawn's Early Light, Key West, Mississippi Delta, Brother Stefan or anything else that Olga Petryszyn creates.
This list may change next month.
My top 10 from MY small (so far) garden would be...
1 - Regal Splendor. (Growth rate, habit, color. Amazing hosta.)
2 - Sagae. (Becomes a show-stopper as it matures!)
3 - Twilight. (Underrated, IMO. The coloring is beautiful and it grows well for me.)
4 - Blue Ivory (The bluest blue, the whitest white. Stunning.)
5 - Krossa Regal. (It's a classic for a reason!)
6 - Golden Tiara. (Small, tidy, pretty. Grows like gangbusters.)
7 - June (Another one that's a classic for a reason.)
8 - Queen of the Seas. (New to me, but there's nothing not to love.)
9 - Paradise Island (Also new, but it became an instant favorite. Decent substance, dimpling, amazing color!)
10 - Clovelly (Also new to me, but good gods is it gorgeous and growing beautifully.)
This list could change at any moment, but that's it in THIS particular moment. :)
Adding to my previous list of '09:
Satisfaction (awesome wide yellow margins, stands med/tall with draping leaves--I have 3 of them now--I'm SO satisfied)
American Icon (very substantial leaves, longish with nice yellow margins)
First Frost (love, love, love it--nice blue with creamy yellow margins espec. right now)
Atlantis (Abba Dabba Do on steroids)
Blue Jay (nice aqua blue and smallish)
Pizzaz (thanks to Phil's pix and recommendation--very nice bluish green with crinkly cream edges)
Carnival (that one has surprised me--I have it in a very bright location and it's just an awesome sort of leaf with a cream edge, slightly undulating and round)
Climax (definitely a climax of my garden as you round the bend of our octagonal deck in the yard--nice limey margins and big leaves in my setting)
Flemish Sky (love it--gorgeous Mediterranean/pale turqouise blue, undulating and pointy leaves that multiplies rapidly for me)
Gunsmoke (nice grayish blue and ruffly long leaves)
Island Charm (bought it but wasn't sure I'd love it, but I do--very small, a neat mound of dark margined with gold centered leaves)
Katie-Q (a gift from diggindutchman with an order and a side of choco-chip cookies and I'm absolutely loving it with it's smaller leaves coming to a point with wide gold margins and multiplies like crazy for me)
One Man's Treasure (Naylor's told me I'd love it and I do--stands tall on very dark burgundy pedioles with long dark bluish green and pointing leaves--so lovely in a pot)
Powder Blue (totally awesome big and puckering dark aqua leaves and did I mention BIG?)
Ventricosa Aureomarginata (an oldie but very much a goodie--love the shiny dark green and wide margins of creaminess)
Spartacus (still new--2nd year but looking very good, ruffled edges and all)
Ginsu Knife (if you have it, you know why it's a keeper)
Dick Ward(Wow--it's taken off this year, dark margins light yellow/gold centers--very puckery and the leaves are just HUGE--not S&S huge but BIG)
Pineapple Upside Down Cake (love it's long slender ruffly leaves in a pot on a stand--looks so lovely and different)
Wolverine (have several and they complement so many of my groupings with their wide creamy margins, with dark centers, long and draping mounds that stand out)
Summer Breeze (pretty sure it was on my previous list but I have to still say it's a standout in my garden, intensely golden wide margins with the lovely grassy green centers, more rounded medium-sized leaves)
I have no idea how many I've just listed and so I won't feel I have to cut any, I'm not going to count them! ;o)
1) Jade Cascade - the best ever
2) Montana macrophylla - WOW...this year it blew me away
3) Flower Power (hmm...I see a trend in my choices lol)
4) Krossa Regal - what a stud
5) Garden Party
6) Lady Isobel Barnett
7) Ebb Tide
8) Atlantis
9) Tidewater
- Vim and Vigor
- oops....11...Blue Mouse Ears...how could you not??
- yeah...still going...Metallic Sheen
- Moonlight Sonata
- Sagae
- Monument Circle...for many reasons (thanks friend...you know who you are :)
ok, I will quit now :)
I lost my list of hosta I have when my computer crashed and I haven't got it back yet, but since I only have around 70 different ones I'll try to come up with some favorites off the top of my head.
In no particular order...
Cherub - just the right size, not too big or small
Prairie Sky - great color
June - 'nuff said
Whirlwind - neat and unusual form
Popo - cute little blue and great grower
Great Expectations - I know, but mine has done great
Liberty - awesome in the spring
Salute - like the ones that stand up ;-)
Gypsy Rose - nice color combo
Lakeside Dragonfly - longer leaves and nice form
I can't remember the names of a few others I really like, but it's dark out, so I'm not going out to check the names.
I am not going to list 1o since all of them have already been mentioned. But one I didn't see on the list which lept last year and blew me away was Spring Fling. If you are looking for a large, vase shape with varigated, piecrust leaves, this is the one!
I love the addition of the pictures - nice touch. Here's my top ten - not in order:
Dawns Early Light - magnificent dome shape and ruffles
Moon Lily - Dusty gold and cute
Lakeside Cha Cha - glows in the shade
Great Expectations - Even if I have to treat it like an annual - it is worth it.
Striptease - Definitly one of my garden visitor's favorites
Halcyon - Gorgeous blue that holds up into fall
Coldheart - love the limey color and ripples
Dream Weaver - big lush leaves on pinky stalks
High Society - love the color combination and the way the blue holds up all season
Wolverine - beautiful and can take a lot of sun
I can't stop...
Aristocrat - I agree with everyone else here - a must have
Tough Question. Don't have a lot....so best way for me to answer is to tell you which ones I would definitely replace if I lost them.
Lakeside Shoremaster [Wins the prize in my yard this year]
Hanky Panky
Golden Tiara
Grand Tiara
Touch of Class
So Sweet [Because of the fragrant blooms & vigor]
Captain Kirk
Sum & Substance [Doubt this one is capable of dying..HUGE]
Sorry that's 12, but I'd replace any of them.
Funny how one's list changes over the years. I just looked at the ones I posted in '09 and there certainly are changes.
2 years ago they were:
On to my top 10 list, in no particular order:
Blue Mouse Ears - I call it my desert island Hosta
Holy Mouse Ears - see a theme here....
Sea Octopus
Maui Buttercups
Fire Island
Monashee Blue Cup
Blue Moon
Dream Queen
Twisting The Night Away
This time around they are:
'Blue Mouse Ears'
'Dawn's Early Light'
'Ray of Hope'
'Sea Octopus'
'Fortunei Hyacinthina' - don't know why I left it out the 1st time
'Rhino Hide'
'Maui Buttercups'
'Blue Ivory'
'Fire Island'/'Paradise Island'


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Could Disney Cruise Line be getting new Ships?
Discussion in 'Disney Cruise Line News & Rumors' started by Wendy, Apr 12, 2013.