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Phylogeny and primary structure analysis of fiber
shafts of all human adenovirus types for rational
design of adenoviral gene-therapy vectors
Sebastian Darr, Ijad Madisch, So ¨ren Hofmayer, Fabienne Rehren
and Albert Heim
Albert Heim
Institut fu ¨r Virologie, Medizinische Hochschule Hannover, D-30625 Hannover, Germany
Received 19 June 2009
Accepted 4 August 2009
The fiber shaft of human adenoviruses (HAdVs) is essential for bringing the penton base into
proximity to the secondary cellular receptor. Fiber shaft sequences of all 53 HAdV types were
studied. Phylogeny of the fiber shaft revealed clustering corresponding to the HAdV species
concept. An intraspecies recombination hot spot was found at the shaft/knob boundary, a highly
conserved sequence stretch. For example, HAdV-D20 clustered with HAdV-D23 in the fiber
shaft, but with HAdV-D47 in the fiber knob. Although all shafts exhibited the typical
pseudorepeats, amino acid sequence identity was found to be as high as 92% (interspecies) and
54% (intraspecies). In contrast to a previous study, a flexibility motif (KXGGLXFD/N) was found in
eight HAdV-D types, whereas the putative heparan sulfate-binding site (KKTK) was only found in
species HAdV-C. Our results suggest that pseudotyping of gene-therapy vectors at the shaft/
knob boundary is feasible, but that flexibility data of shafts should be considered.
Members of thefamily Adenoviridae aredouble-stranded DNA
viruses with a non-enveloped, icosahedral capsid (Swenson et
al., 2003). The 53 human adenovirus (HAdV) types are
grouped into six recognized species (HAdV-A to -F) and a
proposed seventh species (HAdV-G), which were defined
historically on the basis of haemagglutination properties,
oncogenic properties in rodents and DNA homology. The
viruses are mainly responsible for causing respiratory-tract
illness (HAdV-B3, -B7, -B21, -E4, -C1, -C2 and -C5),
gastroenteric infections (HAdV-A12, -A18, -A31, -F40 and
-F41) and ocular infections (HAdV-D8, -D19 and -D37).
Adenovirus capsids consist in total of 240 copies of
trimeric hexon protein, and a penton complex at each of
the 12 vertices. The penton is made of a pentameric base
and a trimeric fiber. The fiber is organized in three well-
defined regions that play an important role in adenovirus
infectivity (Shenk, 2001). These are the amino-terminal
'tail' that binds to the penton base, a central 'shaft' of
variable length and a carboxy-terminal 'knob' domain. The
HAdV-C2 fiber shaft was the first to be analysed in detail
by Green et al. (1983), who suggested pseudorepeats of 15
residues consisting of two short b-strands and two b-
bends. The fiber knob interacts with primary cellular
receptors, e.g. coxsackievirus–adenovirus receptor (CAR),
CD46, CD86 and sialic acid (Marttila et al., 2005; Roelvink
et al., 1998; Seiradake et al., 2009; Short et al., 2004; Sirena
et al., 2004). Primary binding to cells is followed by cell
entry after interaction of the RGD tripeptide loop
extending from the penton base with cellular avb3or avb5
integrins (Bai et al., 1994; Mathias et al., 1994; Schoehn
et al., 1996; Wickham et al., 1993). The flexibility of the
fiber shaft seems to be essential to bring the penton base
into close proximity to the secondary receptor after the
primary receptor interaction. Two flexibility loops have
been described in the fiber shaft of most HAdV types, but
they have not been found in species HAdV-D (Chroboczek
et al., 1995). Furthermore, a putative heparan sulfate-
binding site [KKTK or, in general, BBXB and BBBXXB,
where 'B' stands for a basic amino acid such as Lys, Arg or
His (Templeton, 1992)] was found in the fiber shafts of
species HAdV-C serotypes, but not in other HAdV species.
The virus enters host cells by a process termed receptor-
mediated endocytosis (Maxfield & McGraw, 2004) via
clathrin-coated pits using dynamin-mediated endocytosis
(Li et al., 1998; Morgan et al., 1969; Nemerow & Stewart,
1999; Wang et al., 1998). Cell entry is followed by an
interaction with cytosolic molecular motors, which drive
the capsid along microtubules (Mabit et al., 2002;
Suomalainen et al., 2001) through the microtubule-
organizing centre to the nucleus (Kelkar et al., 2004).
Even though the adenovirus fiber shaft is essential for
infectivity and understanding of virus tropism (Nakamura
et al., 2003; Roelvink et al., 1998; Seki et al., 2002;
The GenBank/EMBL/DDBJ accession numbers for newly generated
sequences are FM210540–FM210562 and FN397565–FN397571.
Supplementary figures and tables are available with the online version of
this paper.
Journal of General Virology (2009), 90, 2849–2854
DOI 10.1099/vir.0.014514-0
014514G2009 SGMPrinted in Great Britain 2849
Page 2
Shayakhmetov & Lieber, 2000; Smith et al., 2003; Vigne
et al., 2003), a complete dataset of fiber shaft sequences has
not been available to date. In this study, fiber shaft
sequences were generated and a complete dataset of all 53
HAdV types and eight simian adenovirus (SAdV) types,
which belong to the HAdV species, was used to study the
primary structure of the fiber shaft and to predict flexibility
loops and heparan sulfate-binding sites. Furthermore,
clustering of the fiber shaft region was compared with
that of the fiber knob in order to search for recombination
events in the evolution of HAdV types.
HAdV prototype strains were obtained from the ATCC,
except for HAdV-B14, -D10, -D13 and -D30 prototype
strains, which were obtained from our collection at the
German National Reference Laboratory for Adenoviruses,
Hannover Medical School, Germany. DNA was extracted
with a Qiagen blood kit. Fiber shaft amplicons for species
HAdV-D were generated by PCR using primers displayed
in Supplementary Table S1 (available in JGV Online). PCR
products were separated in 2% agarose gel and extracted
from the gel with a Qiagen gel extraction kit. Cycle
sequencing was performed with PCR primers and rhoda-
mine-labelled dideoxynucleotide chain terminators (ABI)
and an ABI Prism 310 or ABI 3130 automatic sequencer.
The generated fiber shaft sequences were deposited in
GenBank under accession numbers FM210540–FM210562
(see Supplementary Table S2, available in JGV Online).
Multiple alignments were generated by sequential pairwise
alignment using the CLUSTAL algorithm implemented in the
BioEdit software package (version 6.0.5; Hall, 1999) and
adjusted manually to conform to the optimized alignment
of deduced amino acid sequences. Phylogenetic trees of the
fiber shaft region based on nucleotide positions 31162–
32239 (referring to the HAdV-C2 fiber shaft sequence;
GenBank accession no. J01917) were constructed with the
help of MEGA software (version 4; Tamura et al., 2007) with
several phylogenetic-reconstruction algorithms (neighbour
joining, minimum evolution and maximum parsimony)
and were congruent in overall structure (Fig. 1 depicts
neighbour-joining trees).
Sequences of adenovirus fiber shaft clustered according to
the species concept, as observed previously for the hexon e
determinant, the penton base d determinant and the c
determinant of the fiber knob (Madisch et al., 2005, 2007).
Clustering was supported by high bootstrap values.
Interspecies variability in the shaft region was higher (55–
93% amino acid sequence divergence) than in the fiber
knob region (50–59%) (Madisch et al., 2007); in contrast,
intraspecies divergence was higher in the knob region (18–
53% amino acid sequence divergence) than in the fiber shaft
(18–44%). Surprisingly, all HAdV species except for HAdV-
B showed a lower intraspecies ratio of synonymous/non-
synonymous (S/N) mutations in the fiber shaft (e.g. HAdV-
D, 2.2) than in the fiber knob (e.g. HAdV-D, 3.03). This
suggests that more positive selection occurs in the fiber shaft
region, although the fiber knob contains a well-established
immunogenic determinant (c).
Both the tail/shaft boundary and the shaft/knob boundary
were highly conserved throughout all adenovirus subtypes,
with a completely conserved GVL sequence and a conserved
TLWT motif, respectively. However, species HAdV-F has
two fibers of different lengths expressed at a relative ratio of
1:1 (Albinsson & Kidd, 1999; Favier et al., 2002; Kidd et al.,
1990, 1993; Yeh et al., 1994) (Supplementary Fig. S1,
TIWS sequence as the fiber shaft/knob boundary and an
amino acid sequence identity of only 41% to the long shafts,
suggesting that one of the fibers was acquired from another
HAdV species (Kidd et al., 1993) (Fig. 1a). As only the long
fiber is able to interact with CAR (Roelvink et al., 1998), the
short one may bind to an additional, as-yet-unknown
receptor mainly expressed in the gastrointestinal tract
(Favier et al., 2002), explaining the tropism of HAdV-F.
Comparison of phylogenetic trees of fiber shaft and knob
suggested several intraspecies-recombination events in the
phylogeny of 10 (of 33) HAdV-D prototypes, because of
different clustering confirmed by high bootstrap values
(Fig. 1). For example, HAdV-D20 clustered with HAdV-
D23 in the fiber shaft region, whereas it clustered with
HAdV-D47 in the fiber knob region. Bootscans were
performed with the software SimPlot (version 3.5.1; Lole
et al., 1999) with a window of 200 bp (20 bp step) based on
a Kimura two-parameter substitution model (Kimura,
1980) with a transition/transversion ratio of 2.0 (Fig. 2).
Bootscan results determined a recombination hot spot at
the shaft/knob boundary [nucleotide position 440, referring
to HAdV-D20 (GenBank accession no. AJ811444) in a
complete fiber alignment], a highly conserved region
that is prone to promoting homologous recombination
(Supplementary Fig. S2, available in JGV Online). These
recombination events in species HAdV-D, with several
prototypes sharing identical shaft sequences, suggested the
feasibility of a fiber knob-replacement strategy for
pseudotyping of gene-therapy vectors with a species
HAdV-D-derived fiber gene. As the fiber knob contains
the major receptor-binding site, fiber knob pseudotyping
holds promise to modify tropism. Species HAdV-D-
derived gene-therapy vectors were developed recently for
the treatment of malignant melanoma and soft-tissue
sarcoma (Hoffmann et al., 2007, 2008).
Furthermore, we included seven intermediate HAdV-D
strains (HAdV-D15H9, -D17H29, -D19H30, -D30H44,
-D37H13, -D37H17 and -D46H13) in our phylogenetic
analysis. Previously, these strains had been typed with
contradictory results by neutralization testing (hexon e
determinant) and haemagglutination-inhibition testing
(c determinant of fiber knob), indicating at least one
recombination event between the hexon gene and the fiber
knob-encoding region. However, a detailed genetic analysis
of these intermediate strains had not yet been performed.
Only one of seven HAdV-D intermediate strains (HAdV-
D19H30) was recombinant at the fiber shaft/knob
boundary hot spot (Supplementary Fig. S3, available in
JGV Online). Surprisingly, the fiber shaft of HAdV-
S. Darr and others
2850 Journal of General Virology 90
Page 3
D19H30 was related closely to that of HAdV-D49, whereas
it clustered immediately adjacent to HAdV-D30 in the fiber
knob region, supportedby
(Supplementary Fig. S4, available in JGV Online). This
result indicated another recombination event in the
genome of HAdV-D19H30 between the hexon gene and
the fiber gene, suggesting a multiple-recombinant HAdV
isolate similar to the recently published novel HAdV type
D53 (Walsh et al., 2009). Recently, frequent recombination
events were also described in the phylogeny of species
HAdV-C field isolates (Lukashev et al., 2008).
high bootstrapvalues
A complete overview of the length of all human adenovirus
fiber shafts was generated (Supplementary Fig. S1). All
fiber shaft sequences were congruent to the cross-b model
of pseudorepeats, which contains two b-strands and two
turns (Green et al., 1983). The highest number of
pseudorepeats was found in species HAdV-A (23) and
the lowest in species HAdV-B (six). Several HAdV types
revealed differences in the length of their fiber shafts in
comparison to other HAdV types of their species, e.g.
HAdV-B16 (30 aa, about two pseudorepeats longer),
HAdV-A31 (31 aa, about two pseudorepeats shorter) and
Fig. 1. Phylogenetic trees (neighbour joining) of the nucleic acid sequences of (a) the fiber shaft domain (1077 bp, referring to
HAdV-C1; GenBank accession no. AC000017) and (b) the fiber knob domain (522 bp, referring to HAdV-C1). Bootstrap
values (%) were generated with 1000 pseudoreplicates. In addition to the newly generated fiber shaft sequences, reference
sequences from GenBank were used; see Supplementary Table S2 (available in JGV Online) for details.
Adenovirus fiber shaft phylogeny
Page 4
HAdV-C6 (51 aa, about three pseudorepeats shorter).
These differences in length may also influence flexibility
of the fiber shaft.
All HAdV-D shafts consisted of eight pseudorepeats with a
length between 140 and 148 aa. HAdVs of species D can be
divided into three groups according to the length of their
fiber shaft. The first group, with fiber shaft lengths between
140 and 142 aa, consisted of types HAdV-D8, -D9, -D15,
-D28, -D43, -D44 and -D48; the second, with a length of
145 aa, consisted of types HAdV-D17, -D19, -D37, -D36
and -D45, and the third group, with a length of 147 or
148 aa, consisted of all remaining HAdV-D types.
The entire fiber shaft dataset finally allows determination of
the absence of a heparan sulfate-binding site (KKTK motif)
in all HAdVs other than species HAdV-C. This included a
search for any general motif, BBXB or BBBXXB (where 'B'
stands for a basic amino acid such as Lys, Arg or His).
The heparan sulfate-binding site was formerly described as
a sufficient receptor for initial binding of several HAdVs
0 50 100 150 200250 300350 400 450500550600 650 700750 8008509009501000
050 100 150 200 250300 350400 450500 550600 650700 750800 850 900 9501000
050 100 150200250300 350400450500550600 6507007508008509009501000
0 50 100 150 200 250
Percentage of permuted trees
300 350400 450500 550600 650700 750800 850 900 9501000
Fig. 2. Bootscan analysis performed with SimPlot revealed recombination events at the shaft/knob boundary in the phylogeny
of HAdV-D types HAdV-D47 (a), -D26 (b), -D20 (c) and -D30 (d).
Fig. 3. Porcupine molecular-dynamic repres-
entation of the fiber shaft/knob boundary
including the second flexibility loop of HAdV-
C2 (a), which was also predicted for HAdV-
D23 (b). For comparison, HAdV-D17 (c) is
depicted, which also had differences in the
adjacent amino-terminal loop (red circle).
S. Darr and others
2852 Journal of General Virology 90
Page 5
(Dechecchi et al., 2001). In contrast, recent studies
demonstrated that the KKTK motif of the fiber shaft plays
only a minimal role, if any, in the binding of heparan
sulfate glycosaminoglycans, but is important for post-
internalization steps of virus infection, especially trafficking
to the nucleus (Kritz et al., 2007).
Adenovirus fiber shafts of all HAdVs except species HAdV-
D contain a different number of residues in the third
pseudorepeat that are thought to allow bending of the fiber
shaft (Chroboczek et al., 1995; Wu et al., 2003). Our
complete sequence dataset of fiber shaft sequences now
demonstrates the absence of this flexibility loop in any of
the 33 species D adenoviruses.
A second flexibility loop (the KLGXGLXFD/N sequence,
where X stands for any amino acid) is located in the
penultimate pseudorepeat of the fiber shaft. After analysing
the fiber shaft sequences of HAdV-D8, -D9 and -D15, it
was assumed that all HAdV-D adenoviruses lack this
second flexibility loop, in contrast to all other HAdV
species (Chroboczek et al., 1995). However, our complete
dataset of adenovirus fiber shaft sequences demonstrated a
KDGGLXFD/Nmotif in
(HAdV-D20, -D23, -D24, -D32, -D33, -D46, -D47 and
-D51), which is only 1 aa shorter than the formerly
described motif.
eight HAdV-D prototypes
The flexibility of the proposed KDGGLXFD/N motif in
HAdV-D23 was analysed in silico in comparison to HAdV-
D17 (without a flexibility loop) and HAdV-C2 (with the
flexibility-loop sequence KLGXGLXFD/N). For this pur-
pose, homology models of the partial fiber protein were
initially produced and visualized by using the Swiss-
PdbViewer protein-modelling environment (version 4.01;
Guex & Peitsch, 1997) and VMD (version 1.8.6; Humphrey
et al., 1996). Based on the crystallography fiber data of
HAdV-C2 (Pdb accession no. 1QIU), predictions for
HAdV-D23 and -D17 were performed by using a structural
alignment to guide the threading of model sequences onto
the known molecular structures using the Swiss-PdbViewer
software. A CONCOORD-based simulation and principal-
component analysis on the resulting ensemble was
performed with the Dynamite web server (Barrett et al.,
2004). A higher flexibility was displayed by the length of
the dark-blue spikes of both HAdV-D23 and HAdV-C2
compared with HAdV-D17 in porcupine molecular-
dynamic diagrams of the fiber shaft/knob boundary
(Fig. 3). This suggested that the proposed flexibility motif
KDGGLXFD/N of species HAdV-D is functional. This may
also imply higher infectivity, as previous studies suggested
that the flexibility of adenovirus fiber shafts was crucial for
adenovirus–receptor interaction (Wu et al., 2003).
In conclusion, positive selection of fiber shaft mutations as
strong as seen in the fiber knob region was indicated by the
high intra- and interspecies divergence and low S/N values.
Moreover, a recombination hot spot was found at the
shaft/knob boundary of species HAdV-D and may be used
in future for efficient pseudotyping in the design of HAdV-
D gene-therapy vectors. Further work is needed to
determine whether differences in HAdV-D fiber structure
influence organ tropism.
We would like to thank Mark van Raaij (Instituto de Biologia
Molecular de Barcelona, Barcelona, Spain) for his help in modelling
the HAdV-C2 fiber X-ray diffraction file and Kelly Te for critical
reading of the manuscript.
Albinsson, B. & Kidd, A. H. (1999). Adenovirus type 41 lacks an RGD
av-integrin binding motif on the penton base and undergoes delayed
uptake in A549 cells. Virus Res 64, 125–136.
Bai, M., Campisi, L. & Freimuth, P. (1994). Vitronectin receptor
antibodies inhibit infection of HeLa and A549 cells by adenovirus
type 12 but not by adenovirus type 2. J Virol 68, 5925–5932.
Barrett, C. P., Hall, B. A. & Noble, M. E. (2004). Dynamite: a simple
way to gain insight into protein motions. Acta Crystallogr D Biol
Crystallogr 60, 2280–2287.
Chroboczek, J., Ruigrok, R. W. & Cusack, S. (1995). Adenovirus fiber.
Curr Top Microbiol Immunol 199, 163–200.
Dechecchi, M. C., Melotti, P., Bonizzato, A., Santacatterina, M.,
Chilosi, M. & Cabrini, G. (2001). Heparan sulfate glycosaminoglycans
are receptors sufficient to mediate the initial binding of adenovirus
types 2 and 5. J Virol 75, 8772–8780.
Favier, A. L., Schoehn, G., Jaquinod, M., Harsi, C. & Chroboczek, J.
(2002). Structural studies of human enteric adenovirus type 41.
Virology 293, 75–85.
Green, N. M., Wrigley, N. G., Russell, W. C., Martin, S. R. & McLachlan,
A. D. (1983). Evidence for a repeating cross-beta sheet structure in the
adenovirus fibre. EMBO J 2, 1357–1365.
Guex, N. & Peitsch, M. C. (1997). SWISS-MODEL and the Swiss-
PdbViewer: an environment for comparative protein modeling.
Electrophoresis 18, 2714–2723.
Hall, T. A. (1999). BioEdit: a user-friendly biological sequence
alignment editor and analysis program for Windows 95/98/NT.
Nucleic Acids Symp Ser 41, 95–98.
Hoffmann, D., Heim, A., Nettelbeck, D. M., Steinstraesser, L. &
Wildner, O. (2007). Evaluation of twenty human adenoviral types and
one infectivity-enhanced adenovirus for the therapy of soft tissue
sarcoma. Hum Gene Ther 18, 51–62.
Hoffmann, D., Bayer, W., Heim, A., Potthoff, A., Nettelbeck, D. M. &
Wildner, O. (2008). Evaluation of twenty-one human adenovirus types
and one infectivity-enhanced adenovirus for the treatment of
malignant melanoma. J Invest Dermatol 128, 988–998.
Humphrey, W., Dalke, A. & Schulten, K. (1996). VMD: visual molecular
dynamics. J Mol Graph 14, 33–38.
Kelkar, S. A., Pfister, K. K., Crystal, R. G. & Leopold, P. L. (2004).
Cytoplasmic dynein mediates adenovirus binding to microtubules.
J Virol 78, 10122–10132.
Kidd, A. H., Erasmus, M. J. & Tiemessen, C. T. (1990). Fiber sequence
heterogeneity in subgroup F adenoviruses. Virology 179, 139–150.
Kidd, A. H., Chroboczek, J., Cusack, S. & Ruigrok, R. W. (1993).
Adenovirus type 40 virions contain two distinct fibers. Virology 192,
Kimura, M. (1980). A simple method for estimating evolutionary rates
of base substitutions through comparative studies of nucleotide
sequences. J Mol Evol 16, 111–120.
Adenovirus fiber shaft phylogeny
Page 6
Kritz, A. B., Nicol, C. G., Dishart, K. L., Nelson, R., Holbeck, S., Von
Seggern, D. J., Work, L. M., McVey, J. H., Nicklin, S. A. & Baker, A. H.
(2007). Adenovirus 5 fibers mutated at the putative HSPG-binding
site show restricted retargeting with targeting peptides in the HI loop.
Mol Ther 15, 741–749.
Li, E., Stupack, D., Bokoch, G. M. & Nemerow, G. R. (1998).
Adenovirus endocytosis requires actin cytoskeleton reorganization
mediated by Rho family GTPases. J Virol 72, 8806–8812.
Lole, K. S., Bollinger, R. C., Paranjape, R. S., Gadkari, D., Kulkarni,
S. S., Novak, N. G., Ingersoll, R., Sheppard, H. W. & Ray, S. C. (1999).
Full-length human immunodeficiency virus type 1 genomes from
subtype C-infected seroconverters in India, with evidence of
intersubtype recombination. J Virol 73, 152–160.
Lukashev, A. N., Ivanova, O. E., Eremeeva, T. P. & Iggo, R. D. (2008).
Evidence of frequent recombination among human adenoviruses.
J Gen Virol 89, 380–388.
Mabit, H., Nakano, M. Y., Prank, U., Saam, B., Dohner, K., Sodeik, B.
& Greber, U. F. (2002). Intact microtubules support adenovirus and
herpes simplex virus infections. J Virol 76, 9962–9971.
Madisch, I., Harste, G., Pommer, H. & Heim, A. (2005). Phylogenetic
analysis of the main neutralization and hemagglutination determi-
nants of all human adenovirus prototypes as a basis for molecular
classification and taxonomy. J Virol 79, 15265–15276.
Madisch, I., Hofmayer, S., Moritz, C., Grintzalis, A., Hainmueller, J.,
Pring-Akerblom, P. & Heim, A. (2007). Phylogenetic analysis and
structural predictions of human adenovirus penton proteins as a basis
for tissue-specific adenovirus vector design. J Virol 81, 8270–8281.
Wadell, G. & Arnberg, N.(2005).CD46 is a cellular receptor for all species
B adenoviruses except types 3 and 7. J Virol 79, 14429–14436.
Mathias, P., Wickham, T., Moore, M. & Nemerow, G. (1994). Multiple
adenovirus serotypes use av integrins for infection. J Virol 68, 6811–
Maxfield, F. R. & McGraw, T. E. (2004). Endocytic recycling. Nat Rev
Mol Cell Biol 5, 121–132.
Morgan, C., Rosenkranz, H. S. & Mednis, B. (1969). Structure and
development of viruses as observed in the electron microscope. V.
Entry and uncoating of adenovirus. J Virol 4, 777–796.
Nakamura, T., Sato, K. & Hamada, H. (2003). Reduction of natural
adenovirus tropism to the liver by both ablation of fiber–coxsackie-
virus and adenovirus receptor interaction and use of replaceable short
fiber. J Virol 77, 2512–2521.
Nemerow, G. R. & Stewart, P. L. (1999). Role of avintegrins in
adenovirus cell entry and gene delivery. Microbiol Mol Biol Rev 63,
Roelvink, P. W., Lizonova, A., Lee, J. G., Li, Y., Bergelson, J. M.,
Finberg, R. W., Brough, D. E., Kovesdi, I. & Wickham, T. J. (1998). The
coxsackievirus–adenovirus receptor protein can function as a cellular
attachment protein for adenovirus serotypes from subgroups A, C, D,
E, and F. J Virol 72, 7909–7915.
Schoehn, G., Fender, P., Chroboczek, J. & Hewat, E. A. (1996).
Adenovirus 3 penton dodecahedron exhibits structural changes of the
base on fibre binding. EMBO J 15, 6841–6846.
Seiradake, E., Henaff, D., Wodrich, H., Billet, O., Perreau, M.,
Hippert, C., Mennechet, F., Schoehn, G., Lortat-Jacob, H. & other
authors (2009). The cell adhesion molecule ''CAR'' and sialic acid on
human erythrocytes influence adenovirus in vivo biodistribution.
PLoS Pathog 5, e1000277.
Seki, T., Dmitriev, I., Kashentseva, E., Takayama, K., Rots, M.,
Suzuki, K. & Curiel, D. T. (2002). Artificial extension of the adenovirus
fiber shaft inhibits infectivity in coxsackievirus and adenovirus
receptor-positive cell lines. J Virol 76, 1100–1108.
Shayakhmetov, D. M. & Lieber, A. (2000). Dependence of adenovirus
infectivity on length of the fiber shaft domain. J Virol 74, 10274–
Shenk, T. (2001). Adenoviridae: the viruses and their replication. In
Fields Virology, 4th edn, pp. 2265–2299. Edited by D. M. Knipe, M.
Howley, D. E. Griffin, R. A. Lamb, M. A. Matin, B. Roizman & S. E.
Straus. Philadelphia, PA: Lippincott Williams & Wilkins.
Short, J. J., Pereboev, A. V., Kawakami, Y., Vasu, C., Holterman, M. J.
& Curiel, D. T. (2004). Adenovirus serotype 3 utilizes CD80 (B7.1) and
CD86 (B7.2) as cellular attachment receptors. Virology 322, 349–359.
Sirena, D., Lilienfeld, B., Eisenhut, M., Kalin, S., Boucke, K., Beerli,
R. R., Vogt, L., Ruedl, C., Bachmann, M. F. & other authors (2004).
The human membrane cofactor CD46 is a receptor for species B
adenovirus serotype 3. J Virol 78, 4454–4462.
Smith, T. A., Idamakanti, N., Rollence, M. L., Marshall-Neff, J., Kim, J.,
Mulgrew, K., Nemerow, G. R., Kaleko, M. & Stevenson, S. C. (2003).
Adenovirus serotype 5 fiber shaft influences in vivo gene transfer in
mice. Hum Gene Ther 14, 777–787.
Suomalainen, M., Nakano, M. Y., Boucke, K., Keller, S. & Greber,
U. F. (2001). Adenovirus-activated PKA and p38/MAPK pathways
boost microtubule-mediated nuclear targeting of virus. EMBO J 20,
Swenson, P. D., Wadell, G., Allard, A. & Hierholzer, J. C. (2003).
Adenoviruses. In Manual of Clinical Microbiology. Edited by P. R.
Murray, E. J. Baron, M. A. Pfaller, J. H. Jorgensen & R. A. Yolken.
Washington, DC: American Society for Microbiology.
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007). MEGA4: molecular
evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol
Evol 24, 1596–1599.
Templeton, D. M. (1992). Proteoglycans in cell regulation. Crit Rev
Clin Lab Sci 29, 141–184.
Vigne, E., Dedieu, J. F., Brie, A., Gillardeaux, A., Briot, D., Benihoud, K.,
Latta-Mahieu, M., Saulnier, P., Perricaudet, M. & Yeh, P. (2003).
Genetic manipulations of adenovirus type 5 fiber resulting in liver
tropism attenuation. Gene Ther 10, 153–162.
Walsh, M. P., Chintakuntlawar, A., Robinson, C. M., Madisch, I.,
Harrach, B., Hudson, N. R., Schnurr, D., Heim, A., Chodosh, J. &
other authors (2009). Evidence of molecular evolution driven by
recombination events influencing tropism in a novel human
adenovirus that causes epidemic keratoconjunctivitis. PLoS One 4,
Wang, K., Huang, S., Kapoor-Munshi, A. & Nemerow, G. (1998).
Adenovirus internalization and infection require dynamin. J Virol 72,
Wickham, T. J., Mathias, P., Cheresh, D. A. & Nemerow, G. R. (1993).
Integrins avb3and avb5promote adenovirus internalization but not
virus attachment. Cell 73, 309–319.
Wu, E., Pache, L., Von Seggern, D. J., Mullen, T. M., Mikyas, Y.,
Stewart, P. L. & Nemerow, G. R. (2003). Flexibility of the adenovirus
fiberisrequiredforefficient receptorinteraction.J Virol 77, 7225–7235.
Yeh, H. Y., Pieniazek, N., Pieniazek, D., Gelderblom, H. & Luftig, R. B.
(1994). Human adenovirus type 41 contains two fibers. Virus Res 33,
S. Darr and others
2854 Journal of General Virology 90


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i want to attend diving school in florida for under-water welding but i have to have a twic card. they listed a drug conviction is a disqualifying offense but you can ... - read more
Apr 10, 2011 · How to Get TWIC Card in Louisiana. The terrorist attacks of September 11, 2001 affected all aspects of government. In response to 9/11, the Maritime ... - read more
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To be auctioned at NO RESERVE at the Lucky Collector Car Auction Spring "Jackpot" Collector Car Auction at the Snoqualmie Casino in Washington on April 4th,... More
1941 Cadillac Series 62 Convertible Coupe AACA National First Place Winner, Desirable Options, Exceptional Conditon Throughout It has been rightly said that, in terms of aesthetics,... More
1941 Cadillac Series 62 Club CoupeThe Cadillac Series 62 is a true classic car with beautiful classic lines from 1941.The Cadillac Series 62 was... More
1941 Cadillac 60S, New paint in 2010 and new upholstery in 2014; good mechanical and running condition; driven very little since restoration. ... More
1941 Cadillac 611941 Cadillac Sedanette fast back. Very desirable body style. Fully restored. Steering wheel professionally recast wood grain completed by Bob Kennedy in... More
1941 Cadillac series 61 4 door sedan. Nice original car with original paint and interior. 346 cu. in. engine with a 3 speed column... More
1941 Cadillac 62 Series Convertible 4 year high point restoration just completed. Gorgeous restoration of a rare classic 346 cubic in. Flathead Engine - Whisper quiet New XYZ... More
GR Auto Gallery is pleased to present this 1940 Cadillac Series 60 Touring Sedan for purchase. While Bill Mitchell was in his early 20's... More
1947 Cadillac Limousine, black exterior, front seats black leather, back seats original tan cloth interior, division window, jump seats, Runs Great! $ 33,900.00, Charles... More
Very desirable, good paint and int, rechromed bumpers, wire wheels, V8, 3spd, 69,000 mi, runs good.... More
War was already on the horizon even as 1941 models were introduced in September 1940 in North America. The United States was already shifting... More
1941 was the last full model year before civilian automobile production ended for the duration as Americas manufacturing capacity yielded to the war effort.... More
From owners research he believes car spent 1941-1980 in Colorado. In 1983 the engine was rebuilt. ( Only about 4,000 miles ago) From 1980-... More
Our 1940 Cadillac LaSalle ``Type 52`` Torpedo 4 Dr.Sedan is the last model year the car was produced. *Just 38,457 original miles !!!... More
Cadillac made significant styling and mechanical changes to it's 1941 model line. Headlights were fully integrated into the front fenders and a horizontal-theme grille... More


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Looking for restaurants open for Easter?
Easton Breakfast and Brunch
Pennsylvania > Restaurants in Easton
Restaurants open for Easter in Easton
Looking for restaurant jobs in Easton, PA?
Colonnade Steakhouse
American Restaurants, Breakfast and Brunch, Steak Restaurants
437 Main Street, Bethlehem
The Breakfast Table
2 Crown Plaza, Easton
Tic Toc Family Restaurant
Breakfast and Brunch, Family Restaurants
2510 Northampton Street, Easton
Granola Factory
518 Long St, Bethlehem
Blue Sky Cafe
Breakfast and Brunch, Cafes, Vegetarian Restaurants
22 West 4th Street Frnt, Bethlehem
The Wise Bean Coffee and Espresso Bar
Bars, Breakfast and Brunch, Coffee and Tea
634 North New Street, Bethlehem
Bakeries, Breakfast and Brunch
1342 Chelsea Avenue, Bethlehem
Easton restaurants by cuisine
Nearby Locations
- Allentown restaurants
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- New York restaurants
- Philadelphia restaurants
- Phillipsburg restaurants
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- Stewartsville restaurants
- Stockertown restaurants
- Whitehall restaurants
- Wind Gap restaurants


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Vortex Viper 10x28 Binocular V210
objects! Viper 10x28 Binoculars are tough, reliable in the worst conditions and compact in size. You ... Vortex Viper 10x28 Binocular V210 another award-winning set of binoculars among all types ... warranty. Specifications for Vortex Viper 10x28 Binocular V210: Magnification: 10 x Objective Lens ... with argon gas to inhibit internal fogging. Package Contents: Vortex Viper 10x28 Binocular V210 Rainguard Comfort neck strap Soft carry case ... Height: 4.7 inches Width: 4.5 inches Weight: 12 ounces Features of Vortex Viper 10x28 ... of hunters and guides. There's nothing better than having a great lightweight and compact binocular when ... can't go wrong with this great set of Vortex binoculars! We are an Authorized US Distributor...
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this is the new look at what hard-core hunters demand. Bushnell Trophy XLT 10x28 Hunting Binoculars feature ... Bushnell Trophy XLT 10x28 Binoculars, with world-class optics and superior durability, ... periods of game movement. Bushnell Trophy XLT 10x28 Waterproof Binoculars also feature tough, streamlined ... : Magnification x Obj. Lens: 10x28 Size Class: Compact Focus System: Center Prism System: Roof Prism ... New Dura-Grip Rubber Fast Focus Soft Touch Thumbgrips EF Glass Package Contents: Bushnell 10x28 Trophy XLT Fogproof Hunting Binocular ... waterproof/fogproof dependability. Specifications for Bushnell 10x 28mm Trophy XLT Hunting Binoculars ... of Bushnell 10x28mm Trophy XLT Waterproof Binoculars: 100% Waterproof/Fogproof Fully Multi-Coated Optics...
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Shock, blame after 19 trampled at German festival
DUISBURG, Germany (AP) -- Throngs of techno fans followed the floats, the dancers and the throbbing music to the festival venue: an old freight railway station that local media estimated could handle 300,000 people.
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Update: XL Apologizes for Misleading Atoms for Peace Instagram Post
By Evan Minsker on January 12, 2015 at 9:25 p.m. EST
Update: XL have taken down the Instagram post. It was meant to be a behind-the-scenes look at what goes on in their office's screenprinting room. The label apologizes for being misleading.
Atoms for Peace have been cooling off following their 2013 LP AMOK. In the interim, Thom Yorke released a new solo album and Radiohead confirmed that they're working on new music. But XL have teased that there's something on the horizon for Atoms for Peace. It's unclear what's coming exactly, but the Instagrammed illustration above features the words "for the benefit of all mankind" and "save the date".
Watch Atoms for Peace Perform "Before Your Very Eyes" in Tokyo
By Carrie Battan on December 20, 2013 at 11:26 a.m. EST
In October, Atoms for Peace premiered the new video for AMOK cut "Before Your Very Eyes" live during their appearance at the Hollywood Bowl. MTV Japan also filmed a live performance of the song at Studio Coast in Tokyo in November—it's live now; have a look.
Watch: Atoms for Peace: "Before Your Very Eyes" Making-Of Video
By Jenn Pelly on October 21, 2013 at 1:07 p.m. EDT
Last week, Atoms for Peace shared their surreal, stop-motion clip for AMOK's "Before Your Very Eyes", in which Thom Yorke's body emerges from a desert landscape. Today, director Andrew Thomas Huang has shared a clip documenting the making of the video. The footage shows Yorke undergoing his transformation by the makeup artists, and also captures the whole team of digital artists, sculptors, and animators in action, bringing the video to life.
Video: Atoms for Peace: "Before Your Very Eyes"
By Amy Phillips on October 17, 2013 at 4:52 p.m. EDT
Last night, Atoms for Peace performed at the Hollywood Bowl. During the show, they premiered the video for "Before Your Very Eyes" from AMOK. Today, Thom Yorke Tweeted, "Aaah Blinkin' Hollywood:) Thanks to everyone who came last night. http://atomsforpeace.info/"
Watch Atoms for Peace's Austin City Limits Festival Makeup Show
By Jenn Pelly on October 15, 2013 at 12:42 p.m. EDT
The Austin City Limits festival took place in Austin, Texas over the past two weekends. The final day of the fest, October 13, was canceled due to flash flood warnings. Thom Yorke, Nigel Godrich, and Flea's project Atoms for Peace was among the bands meant to perform that day. They instead played a different Austin venue, the indoor ACL Live at the Moody Theater. Watch that set in full below.
Watch Atoms for Peace Cover Marvin Gaye's "Got To Give It Up"
By Jenn Pelly on October 10, 2013 at 2:46 p.m. EDT
Photo by Erez Avissar
Last night in Mexico City, the Thom Yorke/Nigel Godrich/Flea band Atoms for Peace played at the Pepsi Center. As Radiohead fansite The King of Limbs Part 2 reports, during a second encore, they performed a cover of Marvin Gaye's "Got To Give It Up". An interesting choice, considering this is the song that Robin Thicke's "Blurred Lines" allegedly rips off. Watch below via TKOLP2.
Watch: Thom Yorke and Flea Chat With Jon Stewart, Atoms for Peace Play "The Daily Show"
By Evan Minsker on September 26, 2013 at 8:20 a.m. EDT
Tonight, Thom Yorke, Nigel Godrich, Flea, and their band Atoms for Peace plugged their album AMOK on "The Daily Show With Jon Stewart". Thom Yorke and Flea chatted about the band's origins in an interview segement and the band performed "Default" and "Harrowdown Hill".
Thom Yorke and Atoms for Peace to Appear on "The Daily Show With Jon Stewart"
By Jenn Pelly on September 20, 2013 at 2:20 p.m. EDT
Photo by Eliot Lee Hazel
Thom Yorke, Nigel Godrich, Flea, and their band Atoms for Peace launch the American leg of their tour in support of their AMOK LP next week. On Wednesday, September 25, they'll make an appearance on "The Daily Show With Jon Stewart".
Listen: Thom Yorke and Nigel Godrich Soundtrack Rag & Bone Fashion Show
By Jenn Pelly on September 9, 2013 at 10:17 a.m. EDT
Photo by Erez Avissar
Atoms for Peace: You Wouldn't Like Me When I'm Angry (Mush Mx) (via SoundCloud)
Thom Yorke has teamed up with the fashion line Rag & Bone once again. Yorke and his Atoms for Peace partner Nigel Godrich have provided the soundtrack for Rag & Bone's latest runway show with a new mix of music. (Yorke has soundtracked the line's shows at least twice before.)
Watch: Atoms for Peace Perform "The Clock" During London Residency
By Carrie Battan on July 26, 2013 at 2:35 p.m. EDT
Tonight brings to an end the Atoms for Peace residency at London's Roundhouse. As part of their partnership with Soundhalo, the platform that publishes high-quality video and audio of live performances, they've shared a video from Wednesday night's show. Watch them do The Eraser cut "The Clock", below.
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WISCONSIN NEWS ROUNDUP: Wausau woman sentenced to seven years prison for stealing from a 95-year-old woman
A Wausau woman will spend just over seven years in prison for stealing 200-thousand dollars from a 95-year-old woman while handling her financial affairs. 47-year-old Laurie Goetsch pleaded no contest in February to six felony embezzlement charges. She was sentenced yesterday in Marathon County Circuit Court. Besides her prison time, Goetsch must spend 12 years under extended supervision. She also has to perform 40 hours of community service.


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- Work 1-888-393-8923
- Riverdale, GA
- 33.576461791992 -84.417030334473
690 Apartments for Rent Near McDonough, GA
Of the 690 apartments in the Atlanta metro, we found exactly 7 apartments for rent in McDonough and 11 apartments nearby. Also find 1, 2 or 3 bedroom apartments for rent in McDonough as well as cheap apartments, pet friendly apartments, apartments with utilities included and more. Use our customizable guide to narrow down options for apartments in McDonough and find exactly what you want during your McDonough, Georgia apartment search.
No listings exactly match your search
Please see these nearby communities.
Waterford LandingExact - 1900 Waterford Landing McDonough, GA 3025333.51954267262 -84.188187709525
Our luxury 1, 2 and 3 bedroom apartments in McDonough, Georgia are just minutes for I-75 and I-675. We are convenient to some of the best shopping and dining. Waterford Landing in the only Eagle's Landing Apartment Community located in the Accredited Union Grove School District. Named Top in the Nation for Four Years by Newsweek! Our luxury amenities include: 9ft Ceilings with Crown Molding, Detached Garages Available, Garden Tubs (In Select Homes), Gourmet Kitchens with Dishwasher.
- Elegant 1, 2 & 3 Bedroom Apartment Homes
- Award Winning Union Grove School District
- Resort Style Swimming Pool
- Cardio Fitness Center & Lighted Tennis Courts
- Vaulted Ceilings/Ceiling Fans/Fireplaces
- Patio/Balcony with Storage
- Intrusion Alarms
- Gourmet Kitchens
- Large Closets
- Private Garage Parking (available)
- Auto Detailing Bay
- Resident Business Center
Amber ChaseExact - 570 McDonough Parkway McDonough, GA 3025333.443615287542 -84.167038947344
Located in McDonough, our gated community offers over sized one, two and three bedroom apartments with fully equipped designer kitchens, private patio or balconies, extra storage and high ceilings. Our affordable apartments are within minutes to major malls, dining and schools. Reserve your new apartment unit with us today!
- Washer/Dryer Connections
- Swimming Pool with Wi Fi
- Henry County Schools
- Athletic Center with Sauna
- Private Resident Movie Theater
- Garages Available
- Pets Welcome
- Instrusion Alarms
- Total Electric
- Extra Storage & Private Patio/Balcony
- Easy Access to I-75
- Walk-In Closets and Garden Tubs
Preston Creek at McDonoughExact - 100 Preston Creek Drive McDonough, GA 3025333.435227 -84.177103
Great McDonough, GA apartment homes with 1, 2 and 3 bedroom floorplans. Our smartly designed interiors offer washer/dryer connections, walk-in closets, ceiling fans and private outside storage. Amenities include two swimming pools, lighted tennis court, car care center, playscape, practice your spike at our sand volleyball court, fitness center, or browse the web to check your e-mail while using our Wi-Fi. . We are close to I-75, shopping, dining, entertainment, and are near Lovejoy and Hampton.
- Built-In Microwaves
- Extra Patio Storage
- Controlled Access Entrance Gates
- Community Clubhouse
- Swimming Pool w/ Sun Decks
- Lighted Tennis Court
- Fully Equipped Fitness Center
- Business Center with Free WiFi
- Attached and detached Garages Available-Remotes
- Resident Activities and Social Life
- Playscape
- Car Care Center
The Crossing at McDonoughExact - 100 Crossing Boulevard McDonough, GA 3025333.421662646719 -84.157151329937
We offer spacious and well-appointed 1, 2 and 3 bedroom apartment homes for rent in McDonough, GA. Location only minutes from I-75, we are convenient to shopping, dining, entertainment and a short 30 minutes from Hartsfield Jackson International Airport.
- Luxurious Gated Community
- Monitored Alarm Systems
- Resort Style Pool w/sundeck and Gas Grill Stations
- Tennis Courts
- Health & Fitness Center
- Ceiling Fans in all Bedrooms and Sunrooms
- Crown Molding, Garden Tubs, Sunrooms
- Wood Burning andf Electtric Fireplaces
- Modernized Laundry Facility/Smart App Technology
- Play Scape, Playscape
- Executive Business Center
- Jogging Nature Trail with Picnic and BBQ Areas
Woodlawn ParkExact - 100 Woodlawn Park Drive McDonough, GA 3025333.43413 -84.1575805
We offer spacious one, two and three bedroom apartment rentals in McDonough, GA. We are close to shopping, dining, and easy access to I-75.
- 1, 2, & 3 Bedroom Apartment Homes
- Sparkling Pool with Free Wifi and Grilling Areas
- Washer/Dryer Connections
- 24 hour Fitness Center with Kids Corner
- Tennis Courts
- Resident Car Wash & Picnic Areas
- Controlled Access Entry
- Detached Garages and Storage Available
- Built in Microwaves
- 24-Hour Emergency Maintenance
- Resident Business & Media Center
- Handicapped Adaptable Units
199 Fashion XingExact - 199 Fashion Xing McDonough, GA 3025233.4444 -84.10071
1010 LABONTE Parkway 1010Exact - 1010 LABONTE Parkway 1010 McDonough, GA 3025333.41907 -84.15446
- 11 Apartments within 10 miles of McDonough, GA
Ashley WoodsNearby - 1050 Rock Quarry Rd Stockbridge, GA 3028133.530989 -84.2781275
Affordable Stockbridge Apartments with rates starting at 1 Bedrooms $634, 2 Bedrooms $587 to $747 and 3 Bedrooms $794 to $818. Our spacious and affordable apartment homes in Stockbridge, GA are located within a beautiful park-like setting with great amenities that include: Mini-blinds,Walk-in Closets, Laundry facility on-site, Washer / Dryer Connections, Dishwashers, Bay Windows, Pantries, Ceiling Fans, Energy efficient appliances, High-Speed Internet Ready and Handicap accessible homes.
- Newly Renovated
- Black Energy Efficient Appliances
- Pool with Clubhouse
- Business Center
- Picnic Area
- Washer and Dryer In Every Home
- 24-Hour Emergency Services
- Handicap Accesible
- Central Air/Gas Heat
- Free WIFI
- On Site Maintenance
- Pet Friendly
Carrington RidgeNearby - 860 Rock Quarry Road Stockbridge, GA 3028133.524758 -84.225765
Carrington Ridge Apartment Homes offers 1, 2, and 3 bedroom apartments in beautiful Stockbrige, GA. Minutes from shopping, dining, I-75, 675 and a short drive to I-475. You will enjoy our State of-the-Art Fitness Center, Total Electric apartment homes, Resort Style Swimming Pool and Eagles Landing School District. Come Home to Carrington Ridge Apartment Homes Today.
- Stunning Interiors
- 9 Ft Ceilings/ Crown molding
- Luxurious Garden tubs
- Washer/Dryer Hookup
- Laundry facility/ Auto Detailing Center
- Tranquil pool with Sundeck
- Oversized Windows and Closets
- Lighted tennis court/ Playground
- Garages/ Additional Storage available
- Controled Access Gates
- Free Poolside WI-FI/ Cyber Cafe
- Accredited School District
Glenwood VistaNearby - 10 Glenwood Way Stockbridge, GA 3028133.5125305 -84.2197835
At Glenwood Vista you'll enjoy quality living in a great location near shopping, dining and major business centers. Our apartment rentals include the following: Spacious One, Two and Three Bedroom Garden Style Apartment Homes, Tile Foyers, Private Patios or Balconies, Full-size Washer/Dryer Connections and Custom-Designed Kitchens. Glenwood Vista offers luxury apartments located near Pleasant Grove Elementary, Woodland Middle School and Woodland High School.
- Controlled Access Gates
- Detached Garages with Auto Remote
- Full Size Washer/Dryer Connections
- State-Of-The-Art Fitness Center
- Laundry Facility
- Community Activity Center
- Executive Business Center
- Swimming Pool with Sundeck
- Ceiling Fans
- Expansive Walk-in Closets
- Garden Tubs
Retreat At Eagles LandingNearby - 900 Rock Quarry Rd Stockbridge, GA 3028133.522719 -84.227662
Retreat at Eagles Landing in Stockbridge, GA offers spacious one, two and three bedroom apartment homes close to shopping, dining, I-75 and great Henry County Schools. Check out our many features and amenities: Award Winning Eagle's Landing School District, Pets Welcome, Beautifully Landscaped with walking paths, Remote Access Garages, Lighted Tennis Court, Resort style pool, Cable & Internet Ready Extended Basic Cable Service Included in Rent, Ceiling Fans, Full Size Washer & Dryer Connection.
- Monitored Alarms
- High Speed Internet Available
- Walk-In Closets
- Playground
- Pool with Sundeck
- Fitness Center
- Fireplace
- Vaulted Ceilings
- Detached Garages Available
- Balcony/Deck/Patio
- Total Electric
- Washer/Dryer Connections
The Reserve at StockbridgeNearby - 115 Rock Quarry Road Stockbridge, GA 3028133.540963381529 -84.225697517395
Located in Stockbridge, Georgia, The Reserve at Stockbridge now offers newly renovated, multiple 2 bedroom floorplans with washer and dryer connections, fireplaces, pool, and pet friendly.
- Washer and Dryer Connections
- Fireplaces
- Walk-in Closets
- Close to Shopping, Dining, and Expressway
- Sparkling Pool
- Sunrooms
- Deck or Patio
- Playground
- 24 Hour Maintenance
- Pet Friendly w/Restrictions
- Fitness Center and Clubhouse
Eastwood VillageNearby - 3005 Eastwood Village Drive Stockbridge, GA 3028133.548447415233 -84.256191551685
Eastwood Village Apartments in Stockbridge, GA provides 1, 2, and 3 bedroom options near I-75, I-675, and North Henry Blvd. Near shopping, dining, etc. Our units include garden tubs, walk in closets, washer and dryer connections, track lighting, dishwasher, garbage disposal, ice maker, all electric appliances and much more. Enjoy picnic tables with bbq grills, playground, walking trails, lighted tennis courts, 24 hour fitness facility, gated pool, on site laundry, and excellent walking trails.
- Custom swimming pool and sunning deck
- Minutes from I-75 and I-285
- Garden tubs
- Sunrooms available
- Guest Suites
- Car care center
- Picnic and grilling area
- High energy-efficient standard
- Pet Walking Areas
- Patio Storage Available in Select Homes
- Lighted tennis courts
- 24 Hour Maintenance, Laundry, and Fitness
Cambridge PointeNearby - 3384 Mt. Zion Road Stockbridge, GA 3028133.550543 -84.281433105469
Nestled in the heart of Stockbridge, our affordable apartment community features beautiful 1, 2, 3, and 4 bedroom apartments for rent. We are a pet friendly apartment community! Stop by our gorgeous property today!
- Large Playground with Green Space
- Sparkling Swimming Pool with Lounge Area
- Picnic Areas with Grill and Volleyball Court
- Ample Parking Space
- On - Site Maintenance
- Split Level Floorplans with Roommate Option
- Full Size Kitchens with Dishwasher
- Tile Entry, Ceiling Fans, Garbage Disposal
- Upgraded Berber Carpets
- Washer / Dryer Connection
- Spacious Walk In Closets
- Refrigerator with Ice Maker, Central Heat and Air
Olivia PlaceNearby - 5220 N Henry Blvd Stockbridge, GA 3028133.546356 -84.249562
Spacious one, two and three bedroom apartment homes in Stockbridge, GA with washer/dryer connections, open bright floor plans, large walk-in closets and much more. Olivia Place Apartments are conveniently located near I-675 and I-75. We take pride in our exceptional school system and walking distance to the neighborhood day care. Henry County School District - Stockbridge High School. Come by today and see why Olivia Place should be your next home!
- New Cabinetry in Kitchen and Bath
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- Located in Prestigious Henry County School System
- Sparkling Swimming Pool
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- New wood ceiling fans
Camden StockbridgeNearby - 1000 Peridot Parkway Stockbridge, GA 3028133.547058105469 -84.271606445312
Nestled in the heart of Stockbridge, our property offers spacious one, two, and three bedroom apartment homes. Our beautiful apartment community is located just minutes from upscale shopping, fine dining, and other main attractions the downtown area has to offer. Stop by our wonderful pet friendly apartment community today!
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Cameron LandingNearby - 3470 Mt. Zion Road Stockbridge, GA 3028133.55479 -84.276729
Our beautiful community, located just south of Atlanta, offers spacious 1, 2, and 3 bedroom apartment homes for rent. We are close to great restaurants, shopping, live entertainment, and more. Call or visit us today for a personal tour!
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Walden LandingNearby - 11015 Tara Boulevard Hampton, GA 3022833.45572 -84.327151
Walden Landing offers beautiful 1, 2, & 3 bedroom apartment homes. Easy access to I-75, downtown and Atlanta Hartsfield-Jackson Airport. Close to shopping, dining and entertainment. Walden Landing is the perfect choice to call home. Enjoy top rated Accredited school systems.
- Spacious Stunning Interior
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- Close to I-75, Airport and Downtown
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Topic: Bloomingdale's
34 Places Where You Can Start Using Your iPhone to Buy Stuff
McDonald's, Macy's, Disney World accept Apple Pay
By Garett Sloane on Oct. 16, 2014 - 3:04 PM EDT
So Apple's best lineup of gadgets is out and its next big business is ready for launch—Apple Pay. The mobile payments service launches Monday and today Apple revealed a list…
Get the Perfect Clothing Fit With a Digital Sizing Booth
Bloomingdale's offering high-tech shopping
By Robert Klara on Apr. 15, 2013 - 10:11 PM EDT
A bastion of fashion since 1860, Bloomingdale's might not be the first place that comes to mind for a high-tech shopping experience. But that's likely to change with the department…
SapientNitro: Major Retailers Lack In-Store Digital Savvy
Bloomingdale's and Sephora are lone bright spots among retailers
By Christopher Heine on Oct. 9, 2012 - 10:30 AM EDT
SapientNitro is releasing a 156-page report today that rates retail brands on whether or not they are using digital signage and other interactive features in their stores well. A number…


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Law school student in the Bay Area! I love traveling to new places, meeting new people, and eating!
I'm currently obsessed with Junot Diaz and my new e-Reader.
One thing I can't do without: running! Almost always training for races so looking to live somewhere where there are good running paths nearby.


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Rawhide: Season 2, Episode 1
Incident of the Day of the Dead (18 Sep. 1959)
TV Episode | Not Rated | | Western
Ratings: 7.4/10 from 32 users
Reviews: write review
Rowdy is tricked into breaking a stallion to pay off a gambling debt by a woman. The horse killed one man and injured others including the daughter of the owner. Rowdy is involved in more than breaking a horse - the break up of a family.
Director:Stuart Heisler
Writers:David Victor, Herbert Little Jr.


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This is a very nice museum, lots of displays inside and out ,many buildings and lots of excited staff/volunteers .
The Aurora area has a neat history worth learning about.
High point of a historical village where you can spend a very pleasant afternoon. The village was a German American communal religious endeavor during the latter half of the 19th Century. On the rail line between Portland and San Francisco, it now has the Portland area's best collection of antique shops, some with small cafes.
Great piece of little known history , Oregon's long ago failed venture into collectivism and communal living. They tell the story well with pictures and words. After the museum , take in all the great antique stores.
I love history and Aurora is a must see town in Northern Oregon, with or without the museum. I enjoyed the presentation given by the guide and I found the buildings quite interesting. Old photos of the street and town give an added feeling of what it might have been like to live in those early settlement times.
We spent the day there during Aurora Colony Days in the summer. The whole community comes out. The museum was set up for children to build candle holders, make their own candles, People were in period dress and they taught just how it was to live in Aurora during its heyday. Very interesting
I visited this place when I was growing up and then took my children there. Now I have gone there a few times because my favorite author, Jane Kirkpatrick, has had some book signings there. i like the quilt shows there and have gone there for the strawberry shortcake social. It's a great place to learn of local history.
The curator was well informed and enthusiastic to share the history ofr aurora. I was a little concerrned about the modifications that had been made to historical artifacts, but they would be inviting to young people who enjoy doing more than just seeing,.
If you are looking for antiques and architectural treasures (small and large) this is the place to go. Warm and welcoming to visitors.
We stopped here on our way home from a day of motorcycle riding. We passed a couple that decided against going in once they saw the admission price, but we decided the money would go to good use - local museum, local history, etc. There were no outside programs that day, but from reading the posted programs, it looks like...
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(based on 79 reviews)
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Most Liked Positive Review
Great little Painter
1st off, get a smaller spray tip. I found a Graco 413 sprayed latex fence stain the best. The included (517) tip emptied a gallon of latex primer on my ceiling in...Read complete review
1st off, get a smaller spray tip. I found a Graco 413 sprayed latex fence stain the best. The included (517) tip emptied a gallon of latex primer on my ceiling in less than 5 mins, and caused the mud in the drywall to swell up. A gallon rated at 250-350 sq foot coverage, went half that distance. GET A SMALLER TIP!2nd Clean the sprayer perfectly after use, and use a storage spray to store the sprayer. Dismantle the gun, filter, and whatever else you feel safe dismantling, and wash everything thoughly.The downer. After painting my house with oil paint, cleaning the sprayer thoughly and storing it for a year (yes, with oil in the pump chamber) I went to use the sprayer. The prime portion worked as stated in the instructions, when I switch to spray, the compressor strained for a bit, then shut down (nice auto shutdown feature by the way, if the exit port is blocked). Long story short, plastic was jamed in the exit port, ball valve. Were it came from I don't know but it damaged the only plastic part in the compressor (the ball support sleeve in the valve). The ball valve was jammed closing the exit. In the process of looking for this problem, I totally dismantled this sprayer. Result is that I am somewhat impressed with the internal construction. The drive motor is an induction motor with a capacitor (no brushes). The control module is a simple TRIAC control, very easy to fix for an electronics novice. The gears are all metal, machine cut, on bronze bearings. The output yoke (that moves the piston up and down) actually rides on needle bearings. The pump body is aluminum. The piston has a hardened steel face, as do both ball valves. The top of the cylinder has a brass screw in seal. All fittings are stainless steel with machine threads (no pipe threads). The plastic case and the painted support bar are all impervious to gun wash (Laquer thinner). This sprayer is similar to but not exactly the same as a Graco X15, of which the service manual is available on-line, for free. Internal parts are slighly different, but external parts like the spray tips are interchangable. The Graco manual is a little more detailled than this sprayers manual, with more helpful information. If you are going to dismantle this sprayer for serious cleaning or repair, you will need white lithum grease and thread sealer (not telfon tape but actual thread sealing paste) to reassemble the pump mechanism. Here's a tip I learned a long time ago, ALWAYS, strain your paint before putting it through a spray gun. Cheap buck store nylon strainers work fine, and accommodate large quanities of paint. The Graco X15 users manual (available online) has a list of spray tips and the types of paint they are used with.This paint sprayer would not be good for small projects as it took me about 2 hours to clean up the unit after use. Painting a 250 Sq ft ceiling, took me less time with a roller than with this sprayer, once you factor in the cleanup (although the sprayer only took about 7 mins to do the ceiling).I usually consider HF tools as single project disposable tools, but this sprayer is definitly a better than average (for HF) quality, and certainly worth the money ()
Most Liked Negative Review
Great deal, but still has some problems.
I am female and I've never used a paint sprayer before, but I read a lot and watched lots of videos online before starting with this. I bought this to spray paint the...Read complete review
I am female and I've never used a paint sprayer before, but I read a lot and watched lots of videos online before starting with this. I bought this to spray paint the ceilings. It worked fine, the whole job is done now and I'm happy with it because this thing was comparatively inexpensive, and it would have been several hundred to hire painters to do it. The first thing I can say is that on my sprayer tip, the "Spray side," which has a triangle on it, always shot out a horrible, strong, narrow stream of spray, like a Super Soaker. I turned the tip 180 degrees to the side that says "Eject" and which is supposedly used only for clearing the tip of debris, and it produced the *perfect* spray mist, just like it's supposed to! I'm not sure what's going on here...Was my tip labeled in reverse?? Only when I turned the tip so that the "Eject" side was facing outward did it spray properly, the regular "Spray side" with the triangle was worthless. How odd.Other problematic things: the hose started to leak at the joint of a factory installed bolt - paint was streaming out of it and it got all over. I quickly turned the machine off, grabbed a wrench and tightened the bolt and then started the machine up again and it worked fine, no more leaking. Later on, after I had cleaned the machine and it was sitting with Pump Armor liquid inside it, it was leaking where the suction hose attaches to the machine. Perhaps the clamp was a little loose, but I had not taken it off, it came that way. These leaks are factory issues, issues of quality. Something to be aware of when you buy a sprayer for cheap. But like I said, my ceiling job is done and I'm happy that I purchased it because in the end it saved me a lot of money by doing it myself.
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Best airless sprayer for the money!
By Grams
from Tallahassee, FL
About Me Avid Do-It-Yourselfer
Comments about Krause & Becker 5/8 Horsepower Airless Paint Sprayer Kit:
I don't know all the technical jargon like my husband but I can say this has done a great job on the 2,000sqft exterior of our house. I to purchased the smaller spray nozzle at another store. The only thing is the outer edges of spray from the nozzle are a bit too strong so you need to be careful. Just clean well after each use and your good to go again. Can't wait to use it on interior!My husband says it's one of the best birthday presents I've ever given him.
Bottom Line Yes, I would recommend this to a friend
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(2 of 2 customers found this review helpful)
best $200 ever spent
By soda can Dan
from Milwaukee Wisconsin
About Me Professional
Get the graco 413 tip and you will have the fastest sprayer for the money. I would buy several more if needed. Get the extended warranty just for safe measure and to clean, spray a lot of clean water threw it and clean the tip. You can get the tips from a lot of places just call around It is my first one but I can't live without it. I am a contractor in Milwaukee buy one even if it is hard to get the cash together you will not be disappointed.
(3 of 4 customers found this review helpful)
Perfect for the homeowner
By Zenraider
from Seattle
Got this on sale with 20% off from mailer, made it affordable with extended service plan. Worked excellent right out of the box. Easy to follow clear instructions. Made painting the inside of my house a breeze. Make sure you follow the cleaning procedures or it voids all warrantees. I picked up a bottle of premium graco pump preservation fluid from the big box home store for $8 so theres no reason to cheap out on keeping this thing clean.
(1 of 1 customers found this review helpful)
Great Paint Sprayer
By Trish
from Mechanicsville MD
About Me Casual Do-It-Yourselfer
Great Buy! I was going to rent a paint sprayer from home dump but at this price I decided to just buy. I used it to paint my basement ceiling and it worked great! Very simple to use and hopefully I cleaned it up right so I can use it time and time again.
Great little sprayer
By Kaid painting and drywall
from Alton IL
I bought this sprayer a year ago and was a little skeptical about it because of the low price point. Needless to say i was wrong and it has served me well. I am a pro painter (13 years) and needed something to spray basements and exteriors without breaking the bank. It does the job and then some. It says on the box "for use 6-8 times a year". Well ive used it 20+ Times and its still running like a champ. The gun alone is worth $100. In fact it is the same gun as the graco g-2 without the g printed on it. Eventually i will step up to a pro pump but this is more than i can ask for in the $200 price point. For anyone thinking about getting this, DO IT!!!! Only issue i have found is using bigger tips (517 and up). Runs pretty hard. But its a small pump so you cant ask too much out of it.
Great sprayer
By Tim the Tool Man
from Provo, Utah
This sprayer worked perfectly as advertised!! Best price, excellent quality. I recommend it to anyone.
(2 of 6 customers found this review helpful)
Harbor freight for the win!
By John
from Buffalo NY
Got a good deal on a milwaukee sprayer and it died the first night. Bought a graco and it couldn't suck paint out of the can...figured I would try the harbor freight and save some money while I was at it...it worked great. Some pros of this sprayer is that it has a swivel on the cord which the graco does not. It has plenty of power and is very easy to prime. The tip it comes with is a bit big for most things so you might as well buy a smaller one unless you're painting something that needs a thick/wide coat of paint. One complaint I have is the ridiculously short cord....it's like 18 inches long...not giving you much distance away from the sprayer and the paint/water that you're spraying or cleaning with. Thank you harbor freight...now I can my girlfriends front door back on her house.
(3 of 3 customers found this review helpful)
Works as good as any I've used
By Aug61945
from Kansas
Works Great, I have painted 2 metal garages, 2 2-story houses and 1 ranch style home since I purchased this sprayer.I tip it down around windows for more control and when I want volume I go up on the size of the tip. It has plenty of pressure to break up the paint ( the term escapes me right now )but its worth every penny. JUST REMEMBER IF YOU WANT IT TO LAST DO A GOOD JOB OF CLEANING your paint tools and a little gun oil never hurts!!!!!!!!!!!!!!!!!!!!!!!
very good deal
By jimmyzip
from Youngstown ohio
I paint many rental properties and I bought this when my paint sprayer that I paid more than twice for died after 9 months of use I am much happier with the way this one works and it is easier to clean up after the job is done I have already done 3 houses with it in less than a month and am very satisfied it is a little tricky getting the last of the paint out of a 5 gal bucket but I just added a little pick up hose extension and the problem is fixed and I also use disposable paint screens over the pick up tube to help with clogging also all of my magnum graco parts work with it
great value
By Handy man Al
from Wittmann, AZ
I painted my stucco sided house, carport and patio ceilings with this unit. It preformed very well, no issues. I did buy a 24" extension for the spray gun which kept me off the ladder.
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Snapdeal brings a rich combination of style & class from the house of Mall 4 All. The brand endeavours trendy assortment of jeans, shirts, tees and trousers in variant style and shades. One of the best examples of quality and fashion Mall for All collection is a must for your wardrobe. Choose from our men's clothing section to catch that cool look!
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Thousands of people use Tattoo.com every day to discover a custom design, request a tattoo estimate, or book an appointment with a professional tattoo artist. Our goal is to make finding an artist worthy of your ink fun, fast and easy.
Jinx has just relocated to Fort Collins from Southern California. He has over 23 years of professional tattoo shop experience. He specializes in both Neo-traditional and color realism. His work has been featured in several national magazines and he has won 17 national and international awards. Jinx is also our shop Manager.


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"3D cave surf Cave Surfers" is a new 3D skateboarding game, dark, mysterious, fear, excitement, you dare to challenge it? Tap the screen to control the left and right sides of the forward direction of the little boy, you have to react fast enough, the slightest mistake, the poor little boy will fall into a bottomless pit, oh!
Tap, pinch, and swipe your way to victory in this revolutionary touch-screen wrestling game. Every step taken, every punch thrown, and every move executed is now more satisfying than ever thanks to the intuitive control system. And with up to 10 characters on screen and dozens of fully interactive items and weapons, its the wildest action your mobile device can handle.
ICC world twenty 20 - is an official version of ICC World Twenty 20 for mobiles. Real stadiums from all over the world, change of day and night, weather conditions, onscreen repeats amd many other things! Game features: 2 control modes Quick game mode and championship 6 stadiums
this game is Pro evolution soccer 2012 for mobile java phone. Get the times of every competition of PES 2012 online master league. You can filter on your division and preferred mode and set notifications when a competition you want to play is about to start, or tell a friend about it. and is by the version euro 2012 on this site you can download www.pesmobile.tk
Christmas Special- Try this new Hill Climb Racing with better performance and exciting new Neon level. This game is awesome and different from all the games you have ever played. You have a robotic car to drive on a narrow path in ice fall region. You have to control your car while maintaining speed and balance. There are lots of power boosters in this game but you should use them only when you need otherwise your car fuel will decrease.
This game brings you the fast paced and exhilarating experience of Cricket on your mobile phone with intuitive gameplay and simple controls. Capturing the true essence of Cricket T20, this game is about quick scoring, battling crunch situations and strategic display of skills. Featuring 6 Topnotch stadiums across the world, with day/night & weather choices, pitch selections, lively cheerleaders and cinematic replays to keep the atmosphere intensifying.
Fan Testimonials ***** "Awesome game with best features, it is a real 3D game to play. Even better graphics..loved it..." ***** "Awesommmeeeee!!!!!! Love this game. Top ratings from me for giving MSD signature Helicopter Shot..." Update: New version available now with brand new look and HD graphics for a never seen before Cricket game experience! The premier game of Cricket is on the verge of an Epic Battle! Presenting Cricket Play 3D, a top class cricket game which has all the thrill
Ships GTZ- Free Download Ships GTZ empowers you to have a Super Cool and thrilling 3D Ship Racing/driving experience. This game will push your driving instincts to their limits. Focus on your ship and try to drive through the various cool water beach tracks to overtake other ship racers and lead the track. Game challenges experienced racing players as well as newbie's Its an entertaining sports ship racing game with rich graphics where you have to drive the ship and defeat other traffic
Experience our first ever multiplayer cricket game! "Cricket Play" is the most complete cricket game for your Java Phone. Play with your favorite cricket national team. The games will transport you into the arena of international cricket. If you are in the mood for some mischievous fun, then Fantasy Cricket League will give you plenty of opportunities to vent your devilish streaks! Download this FREE Java app.
Cricket Predict and Win is a skill-based cricket game. Use your knowledge of the game, and your rating of the teams, to predict various possibilities- who will win the featured match, who will score the most runs or get the most wickets - the possibilities are endless. Get India vs West Indies live scores, schedules, latest cricket news, team and player stats and predict outcomes to win amazing prizes like LCD TVs etc.
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Lives in Montréal, Québec, Canada
Works as a Lawyer
Joined on Jan 27, 2009
That's.. different! I like it.
Can't wait to go see this in person!!!
If I rely solely on my latest experiments with the K-3, a formidable "APS-C" DSLR in my opinion (if not the ultimate!), this can only bode well for all Pentaxians or other open-minded photographers and enthusiasts.
I love the idea of some 'M' diversity! My GXR doesn't...
Interesting, dare I say "daring": having followed the Leica digital rangefinder development saga closely, I have no doubt about the many challenges facing this Konost team. Now, would this be enough to entice Cosina to resurrect any kind of Zeiss Ikon 'D' project!?
My daughter wants that bird.. Please.
The E-1 still impresses! Nice catch.
Ricoh's quite smart: the whole Pentax legacy needed to build itself a substantial (D) FA(*) zoom lineup in order to move on towards that imminent FF launch, as well as to dust its somewhat aging SMC DA(*) wide-to-normal zoom offering. With no sweat nor threat, things are starting to take shape in a very promising manner for both APS-C and FF camps of the same tenacious K-mount!
Victor12, This area looks familiar... Well captured!
That man, speaking on behalf of.., has a task to fulfill. That, I know. My wishes? Choice, as in variety. Samsung cams operate like a song, I agree! That man is very capable, also. Obviously. Speaking on behalf. At DPReview. To every single one.
That man is very much wishing for DSLRs to vanish entirely from the face of Earth, obviously not having anything to sell in that camp...
As if sensor sizes were the only IQ catalysts..!
Wow, amazing beasts! But what are they???
So cute!
The title is quite sarcastic: "Not Dead Yet"!? Could say that to Nikon and Canon first... Miss my A700, would buy another one anytime if found in store brand new!
(Speaking from experience, from Alpha line upward...) Sony's manufacturing reliability has been abysmally bad in photo gear, whereas its technological accomplishments have been rather impressive on all fronts. I guess that the whole point of this joint venture Hasselblad-Sony was to bring promising products much closer to perfection!
plainwhite: I might have been tempted to purchase the Typ 113 if it had a built in VF and full manual control of the ISO and aperture setting at f/1.7. But alas, there are to many compromises for $3k in my opinion.
Just because a person has money, doesn't mean he shouldn't also have some common sense.
my 2¢
Opt for Type 109...
I enjoyed (and trusted) this farmed-out review format, DPReview; please, keep it that way!
Nice eyes!
But will it perform better than the rest of the X family with M-Mount lenses?


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Mr. O'Hanlon has served as my legal counsel on everything from auto accidents, to estate planning and real estate issues. I've worked with him as...
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Mr. O'Hanlon was recommended to me by an attorney that I've worked with in the past because he speacialized in the type of legal assistance that I...
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Top-notch attorney. Highly respected in the legal community.
Sean is an effective and zealous advocate. His clients benefit from his demeanor and relationships with other attorneys to achieve the best possible...
10 years since Sean Patrick O'Hanlon was first licensed to practice law.
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Holy Malevolence
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So I'll be able to get Rammy as well.
woot I guess I know who Im buying soon
3150. Bought her with the 4k IP I had and I still have 1300 for runes. YAY!
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Dota 2 is an action real-time fantasy strategy game and the stand-alone sequel to the popular Defense of the Ancients modification for Warcraft III: Reign of Chaos and The Frozen Throne mod Defense of the Ancients. The game combines the real-time strategy element of traditional top-do...
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Platforms: PC, XBOX
Genre: Role-Playing , Action
Publisher: Electronic ArtsDeveloper: BioWare
Esrb rating: Mature (17+)
Released: May 28, 2008
About: Mass Effect is an action role-playing game. The game takes place in the year 2183, with the player, assuming the role of an elite human soldier named Commander Shepard, setting out to explore the Galaxy on a starship, the SSV Normandy. The titular mass effect is a form of inertia-suppressing technology, allowing faster-than-light travel. The game features four classes of conventional firearms (pistols, shotguns, assault rifles, and sniper rifles) and grenades, along with a variety of weapon and armor upgrades.
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Mass Effect (PC)
The Secret World is a massively multiplayer online role-playing game. The game will feature an original setting created by Funcom, with contemporary elements as well as magic, myths, conspiracies and dark horrors. The game will blend elements of MMO gameplay with alternate reality gam...
Dragon Age II is a role-playing video game. It is the sequel to Dragon Age series. The game is set in the same mythical world introduced in Dragon Age: Origins, the player assumes the role of Hawke, a human mage, warrior, or rogue who arrives in the city of Kirkwall as a lowly refugee...
Fallout: New Vegas is a role-playing video game in the Fallout series. The game is based in a post-apocalyptic environment in and around Las Vegas, Nevada. The game is not a direct sequel to Fallout 3, though the game offers a similar role-playing experience. The game places the playe...
Dragon Age: Origins – Awakening is an expansion for the fantasy role-playing game Dragon Age: Origins. Awakening adds a brand new campaign which takes place during the aftermath of Dragon Age: Origins. The game also features new class specializations and skills for the player to dev...


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Grasston, MN, United States | Member Since 2009
"Good pre-view in audio format"
On its own, audio not optimal for a weightloss/lifestyle book
not applicable
Narration was fine.
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I like the theory of the book, not really unusual--but the author did use science to substantiate his claims.
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Looking for Pedro Dot Roses
Roses like Catalonia, Girona, Mari Dot, Ibiza or Angel Guimerà are really hard to find in Spain. Dot nurseries doesn't grow this kind of roses.
I' have roses breeded by his son, Simon Dot, for instance, Tarde Gris, MÃstica, Centro de lectura, Rosaolor, Rose Dot.
I'd like to swap cuttings, if is possible.
I'm sorry I can't help you with the Dot roses, which aren't in my area of rose interest, but I did want to welcome another European member to the Antique Rose forum. I'm an American living in northern Italy in the hills with a large young rose garden, mostly older and antique varieties, or English roses. How about yourself?
Sorry, I spend most of my time on the Antique Rose forum and that's where I thought I was. You might want to put your question there. I know there are participants who are interested in the Pedro Dot roses, and they might be able to give you a hand. At least you'd have fellow enthusiasts. That forum and the Rose forum are more active than the Propagation forum.
Thanks Melisa. Ti posso scrivere in italiano? Cosi posso esprimermi meglio.
Sono catalano, vivo in Barcelona, e ora sto in cerca di quelle rose dimenticate per i nipoti de Pedro Dot. Qui non ci sono molti tiffosi delle rose, pertanto, loro mi hanno detto que soltanto fanno crescere quelle varietà che possono vendere facilmente. Tutte quelle di Pedro Dot, dei anni 30, non posono reprodurle perche non hanno il materiale vegetale necessario.
Grazie Melissa


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The Children of Heaven
Majid Majidi
This film which pictures the love and affection between young Ali and his little sister Zahra, was the first Iranian film to be nominated for an Academy Award for Best Foreign Language Film. The film is set in Tehran where little Ali and his poor family live. Ali loses her sister's shoes, so they have to share a pair of school shoes. Ali tries so hard to come third in a children's footrace and win the prize of a pair of sneakers for his sister, but is disappointed when he accidentally comes first in the race. The simplicity of a child's world and the deep love and compassion prevalent in it lights up the whole movie.
The Great Debaters
Denzel Washington
The film is based on the true story of a debate team from the African-American Wiley College beating Harvard in the 1930s when racial discrimination was rampant and Jim Crow laws were in vogue. The film has Denzel Washington as Prof. Melvin Tolson of Wiley College, the brilliant but volatile debate team coach along with co-actors Forest Whitaker, Nate Parker, Jurnee Smollet and Denzel Whitaker. Tolson coaches and inspires the college team through a nearly-undefeated debate season to finally challenge Harvard in the national championship.
The Bucket List
Rob Reiner
This film is the story of two terminally-ill men, a billionaire and a mechanic, played by Jack Nicholson and Morgan Freeman, who escape from the cancer ward and venture on a road-trip to accomplish their wish list before they die. They go around the world, sky dive together, climb the Pyramids, fly over the North Pole, eat in the best restaurant in France, visit the Taj Mahal, travel to the Great Wall of China and go on a lion safari in Africa. They reflect on life and the questions from Divine Comedy, "have u found joy in life?" and "have u brought joy to others in life?" and this gives them a new insight into their relationships.
The Fall
Tarsem Singh
The film is about a stuntman and a little girl, played wonderfully by Lee Pace and Cantinca Untaru. In the 1920s, in a hospital in Los Angeles, stuntman Roy Walker with a broken leg and heart, charms a little girl Alexandria with his fairytale to get him morphine from the hospital to commit suicide. The friendship that he strikes with the little girl through this fairytale prevents him from committing suicide. This film is a fine blend of fantasy and reality.
Jean-Pierre Jeunet
This is a French film about a waitress in Paris, played by Audrey Tautou, who decides to help people while struggling with her own lonely life and on the way finds true love. One day, Amelie finds an old metal box of childhood memorabilia hidden by a boy who lived in her apartment decades earlier. After considerable effort she returns it to its owner. The characterisation of Amelie is what makes the film unique.
Those that almost made it
Seven Pounds: Gabriele Muccino
V for Vendetta: James McTeigue
The Book of Eli: Albert Hughes, Allen Hughes
12 Angry Men: Sidney Lumet
The Shawshank Redemption: Frank Darabont
(S. Saranya is a Chennai-based lawyer.)
Keywords: World cinema