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"Superman" or "The Superman" ?
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Mace Dolex
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Join Date: Oct 2009
Posts: 5,202
Re: "Superman" or "The Superman" ?
I'd have to watch Nolan's trilogy and keep a score card for how many refer to it as The Batman but in a sense calling him "The Batman" just reinforces the image of fear and urban myth that Nolan tries to create in the films, instead of thinking him as a person the criminals think of him as a thing.
That doesn't work with Superman since he doesn't seclude himself and helps Metropolis law enforcement.
Self-contained stories part of a larger story is where its at. Not this writing for the trade nonsense. If you're going to write for the trade, don't release it in individual issues. Just release the trade.
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A Government race adviser with close links to Cherie Blair is at the centre of a major investigation into an alleged illegal immigrant employment racket which has seen a total of 70 men arrested


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Abstract trumpeter© Lonely - See portfolio
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Parisa Zarin Model
Belkin ImagesPhotographer Female Ogdensburg, New Jersey, US
My Website: Belkin ImagesMy MM URL: http://www.modelmayhem.com/BelkinImages
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Belkin Images
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Art Institute of Pittsburg for Photography with film media and later graduated Kaiser Institute of Technology for computer science.
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Halcyon Castle
Halcyon Castle was built in 1932 in the princely state of Travancore, in the modern-day state of Kerala, India. It was constructed by M.R.Ry Sri Rama Varma Valiya Koil Thampuran, the consort of H.H. Maharani Sethu Lakshmi Bayi, as a retreat for their family.[1] In 1964 the entire property was sold to the Government of Kerala by the Valiya Koil Thampuran, and has since been converted into a deluxe hotel. The castle became disputed when the ITDC (India Tourism Development Corporation) who was running the hotel sold out the hotel to a private Hotel resort group. .[2] The Hotel and the entire adjoining land area at measuring around 65 acres is now owned by the NRI Business man Dr Ravi Pillai who has entrusted The Leela Hotel Group to manage his 5 Star Deluxe hotel.
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- Thu
- Mar 26, 2015
- Updated: 10:33am
Sino-Japanese relations
The relationship between the two largest economies in Asia has been marred throughout the 20th century due to territorial and political disputes including Taiwanese sovereignty; the invasion of China by Japan in the second world war and Japan's subsequent refusal to acknowledge the extent of its war crimes; territorial disputes surrounding the Diaoyu/Senkaku Islands and associated fishing rights and energy resources; and Japanese-American security co-operation.
The mad economic cost of Japan and China's sovereignty row
The territorial row between Japan and China is a threat to both economies but it also poses a big danger to prosperity around the world
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Can China and Japan really be contemplating the madness of going to war over who owns a tiny string of uninhabited rocky islets far from anywhere in the East China Sea?
A couple of months ago, most commentators expressed confidence that the row would soon blow over, but it is continuing with new bellicose words, provocative actions and political tensions between the once and future economic powers.
There is a lot at stake economically, but if Beijing and Tokyo continue on a path of confrontation, the threat will be not merely to their own economies but to global prosperity.
Japan has already felt some of the economic consequences of China's wrath, with sales plummeting for its carmakers and tourism taking a big hit as Chinese visitors cancelled their bookings en masse.
Anger in China towards Japanese got so intense that even possession of a Japanese vehicle became a crime in the eyes of some Chinese street mobs, as one poor motorist found.
In the short term, Japan is suffering more since it is more vulnerable to a Chinese boycott - as the car sales and drop in tourists have shown - and to Beijing's orchestration of such a boycott.
If confirmation were required, it came with the publication of Japan's September trade figures, showing a deficit of US$7 billion as Japan's exports fell by 10.3 per cent. Ominously, it was Japan's first September deficit since comparable figures started in 1979. Worse seems certain to follow, pushing Japan back to recession.
In Japan, it is not so easy for politicians to rouse patriotic fervour by telling people not to buy Chinese products - and it would be a dangerous and provocatively totalitarian step if they did.
Chinese products, from garlic and vegetables to plastics and electronics, are ubiquitous on Japanese shop shelves, up to a third cheaper than Japanese-made equivalents.
China has become an integral and well-integrated part of the supply chain of many Japanese manufacturers, so that they could not boycott China without threatening their whole production line. These factors probably inspired Beijing to try to teach Japan what it hoped was a short, sharp lesson.
In the medium term, China stands to lose both directly and indirectly; Japan may gain from reassessing and redirecting its supply chains.
But the real danger is not merely to the two protagonists but to Asian growth and stability, and to the whole global economy, if sullen antagonism - let alone outright hostility - between the world's second- and third-biggest economies becomes the order of the day.
In the last few years, China and Japan have expanded their investment, trade and economic relations to the point where they have become mutually dependent. Trade between China and Japan reached a record US$345 billion last year, according to Chinese figures.
China became Japan's biggest export market in 2009, taking over from the United States. China takes almost 20 per cent of Japan's exports, compared to less than 7.7 per cent a decade ago.
Japan is China's third-biggest trading partner, after the European Union and the US.
Japanese investment has poured into China. Chinese statistics show that by October last year there were 33,400 Japanese companies and affiliates operating in China, up 75 per cent from the already high figure of the year before. Already in 2010, Chinese statisticians reported, Japanese companies employed three million Chinese and accounted for 16 per cent of all foreign companies in China.
This is not a relationship of Siamese twins. Statistics compiled by Reuters show that Japan's investment in China topped a trillion yen (HK$97 billion) or almost US$13 billion last year, a rise of 60 per cent from the year before. Total Japanese investment in China over the past 15 years has topped US$80 billion.
On the other hand, China's direct investment in Japan is more modest. It fell last year to 8.9 billion yen, from 27.6 billion yen in 2010, but even the 2010 figure is well below the money that Japan has been pouring into China.
The pattern is clear: Japanese companies squeezed by high labour costs and the rising yen have increasingly moved production offshore to China.
The ongoing dispute will no doubt provoke belated examination of the wisdom of Japan putting so many of its investment eggs in the China basket.
By Beijing's careful calculations, it may feel it will have less to lose in an economic fight with Japan. But loss of Japanese confidence and gradual withdrawal of modern Japanese technology would be a high price to pay for Beijing's aspirations to create a modern superstate.
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Vegetable stir fry© Donna Smith - See portfolio
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Exam Proctoring
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CSP Case Manager - MD462
NRI Community Services - Woonsocket, RI
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Hotel Torico Plaza
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La Vuelta, Vuelta al Cole y Vaya a Playa!
... the policy on absences, or how to pay our tuition bill...) they make up for with friendliness and positive energy.
We've discovered our local mall -- a huge commercial center less than 10 minutes away -- that comes alive at night with restaurants, kids entertainment and a 24 screen movie theater. On Friday night we saw Hercules at 7:30 in English with Spanish subtitles. We're working our way up to movies in Spanish...
We packed a picnic and ...
"I think you're telling me a little bull"
... br>
Teruel was a pleasant one hour drive. It is noted for its harsh climate and renowned jamon Serrano. The parking fairy was once again good to them with a park right outside the old historic centre.
It was a lovely day with a top temperature being 28 degrees.
They wandered into the town square and visited El Torico and soaked up the atmosphere as the locals populated the many caf�s in the square enjoying ...
Parked in the valley before climbing to the hotel.
The car park was below the hotel which was pasted to the side of the hill. Our room overlooked the valley so we were able to see that the bikes were all ok. The trip organisers had hired a couple of locals to keep watch all night so no problems encountered with bikes being messed with. Spent some time in the evening walking around the old part of town and visiting the castle at the top of the hill.
A Gorge -ous walkand aTowering viewpoint
... path would be very windy but we seemed to be protected by the walls.
We reached the walls and climbed up some steps ( no wall or handrail!) and could have walked on the top of the wall - but no ...
In and out the alleyways....
... a lovely walk, and only saw one other couple.
The campsite is very good, the pitches are long and narrow and only marked by trees and are very dry and dusty, but the Sanitary block is brand new and the showers are fully adjustable with continuous flow. WiFi is continuous and free, not too strong on the pitches but enough to do this blog! ...


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So about me: I'm fun, outgoing, easy to talk to, witty, social, goofy, handy as well I'm truly interested in meeting a nice young lady to get to know. I've always been relationship minded so I'm looking to build something meaningful. I would like to experience new things and display the more romantic, fun side of me. But I can't do that unless I have a chance to get to know you. If your intersted in a young man who's about something. lets make it happen. What interest me about her. Fun, outgoing, sweet, down to earth, just a good women with a great personality basically
Camping , Cooking , Dining out , Fishing/Hunting , Hobbies and crafts , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Performing arts , Playing cards , Playing sports , Religion/Spiritual , Travel/Sightseeing , Video games , Volunteering , Watching sports
Baseball, Basketball, Bowling, Football, Martial arts, Other types of exercise, Running, Swimming, Tennis / Racquet sports, VolleyBall, Weights / Machines
I exercise 3-4 times per week.
Some other viewpoint
Cold Stone, lake restaurants, lake Calhoun , VA Beach , LA, PA, Baseball Hall of Fame, DC Muesems
Indiana State University, Terre Haute, IN
Working out, relaxing and watching movies, reading, working on cars .
I pretty much like to stay up on technology. I love working with me hands and I enjoy learning new things, I enjoy sporting events traveling, and dining out, etc. I'm always looking to learn new things as well
Seven Laws of Thinking
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|6'1" (185cms)
|4'0" (121cms) to 6'1" (185cms)
|Body type:
|Athletic and toned
|Slender, About average, Curvy, Athletic and toned, Full-figured
|No preference
|Light brown
|No preference
|No Way
|No Way
|Social Drinker
|Social Drinker
|Technical / Science / Computers / Engineering
|No preference
|I'll tell you later
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|Never Married
|Never Married
|Have kids:
|Wants kids:
|No preference
|Black / African descent, Native American
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|Christian / Catholic, Christian / LDS, Christian / Protestant, Spiritual but not religious, Christian / Other
|English, French
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|Bachelors degree
|Associates degree, Bachelors degree, Graduate degree
Electronics Engineering


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Wrestling Do I Need To Get Tested For AIDS
Here's a taste of what TheBody.com has to offer on this topic:
Get the Facts
How to Spot HIV/AIDS Fraud...it requires payment in advance . If a product really works, why isn't it advertised and available publicly? This may be a way to get around government regulations. You can only get the treatment by paying to take part in testing an experimental treatment . When most experimental treatments are tested, people receive them free of charge. Common Sense About Unproven Treatments You might end up spending a lot of money on treatments...
Ask the Experts
Wrestling risk ? 2nd ATTEMPT - Made a donationHello doc,I realize that you get way too many emails and I am hoping you look at this one.. I have also made a donation at http://www.concertedeffort.org to help the cause.A few days ago, I wrestled with a stripper at a private party. She had her...
Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation...bruise. If HIV were contracted this easily, everyone who rides the subway at rush hour would be infected by now. As for HIV testing kits, Home Access Express test kits are indeed sold at pharmacies and online. This requires you use a lancet on... Read more »
HIV, Wrestling, and other SportsWhile I am at this site I would like to pose this question to anyprofessional out there who would like to reply. Wrestling is a sport that Ienjoy very much. Is it safe to wrestle with a person who is HIV positive. Am I placing my life in jeopardy...
Response from Rick Sowadsky, M.P.H.
Nevada State Health Division AIDS Program...arenot permitted to play again until they stop bleeding (this includeswrestling).The only sport that has requirements for HIV testing is in professionalboxing, and even that is only done in certain states. This was never a bigissue until the... Read more »
worried future med studentDr. Bob,First off, you are hilarious!! If you were on TV instead of lame DANE COOK, we'd be so much better off. Also you're a wonderful person who has helped out so many, and we really need more people like you in this world. I've been wrestling...
Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation..."special massage" package might be listed as the "VIPenis"?)3.Nope!4.Right! No risk!5.Nope. No risk!I see no medical reason for an HIV test. So if your logical side can kick the paranoid side's butt, that's all that's required. However, if such... Read more »
suicidal with hivI know I have HIV/AIDS and I want to commit suicide. No blood tests have confirmed this yet but I expect that they will soon. What should I do? It may be easier to end it now than to have to wait on and hear those results. I can't deal with it. ...
Response from Michael Shernoff, M.S.W.
Psychotherapist and Author...hysterical reaction. Life with AIDS is certainly no picnic, but without blood tests you don't even know if you are infected with HIV.I sense that when you began by telling me you have AIDS, you in fact mean that you may find out that you are... Read more »
Women and HIV/AIDS...here . In Conclusion The numbers of HIV women are growing. This means that all women should beaware of the risk and be tested if there is any possible reason to think theymay have been exposed to HIV. If you test negative, you can take... Read more »
What Are the Symptoms of HIV and AIDS?...without your body giving away any sign whatsoever that you have HIV. This is why it's so important for everybody to get tested for HIV regularly (preferably once a year as part of a routine health checkup). Having HIV may be scary, but... Read more »
HIV/AIDS Among Hispanics/Latinos...high among Hispanics/Latinos. Cultural factors may affect the risk of HIV infection. Some Hispanics/Latinos may avoid seeking testing, counseling, or treatment if infected out of fear of discrimination. The stigma around homosexuality adds to... Read more »
Diabetes and HIV/AIDS...high fasting glucose level. A diagnosis of diabetes can be made based on any of the following test results, confirmed by retesting on a different day: A fasting blood glucose level of 126 milli grams per deciliter (mg/dL) or higher A random... Read more »
Connect With Others
Potential HIV risks please help
Posted by 613, 3 Replies...OK, Just for clarification, I have considered taking an HIV test when I get my yearly physical here are my risks 1. Received oral sex from a woman in 2006 (unprotected) 2. fingered the Read more »
Please Help, Confused and scared...
Posted by jrob123, 2 Replies...feel a irratable tingle sensation in my anus. I need help anyone with any feedback is much appreciated. Also I went to get tested after 10 days results were negative, but the nurse told me that its an antibody test and in 10 days there is no... Read more »
He/she is HIV+.......to love or not to love.
Posted by mandaree23, 10 Replies...they should do about the person they are in love with due to the fact that they are HIV+. I want to say that anyone who has tested positive for HIV/AIDS is a person, no different than those of us who are fortunate enough to not have contracted... Read more »
Newly diagnosed. Need to chat, find support
Posted by Amadoda, 6 Replies...experience the problms I read about here in terms of side-effects. Is this too soon to judge? I tend to become very focused being HIV positive and I do not want it to be my life. Any hints as to how one starts to move on? Are support groups... Read more »
Resource Centers
Guides for People With HIV


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Is there any reason you cant force carb at room temperature? I know it would take a higher pressure setting and probably more time but is there anything else? I would like to have my kegs carbed and ready to go when I put them in the kegerator.
Thanks. I like to condition my beer at room temp for a couple weeks after I put it in the keg. I am going to pick up a second regulator and tank tomorrow. It will be nice having the beer carbed and ready to go when I put them in the kegerator.
I just started kegging but have done this on my first 2 kegged batches. I just hit it with 23psi at room temp for a couple of weeks and then purge and set to 12 when it goes in the keezer. I'm not sure what the effects of the pressure are on the yeast (this would be a good experiment), but both beers have turned out great.
I don't for certain have the answer to the pressure effects on yeast question, but here's my thinking: We prime bottles with dextrose, the bottles carbonate, and the beer continues to age and change for months/years, under pressure, in the bottle. So, carbonating a keg and storing at room/cellar temperature would essentially have the same effect (or lack thereof) on the yeast/beer, but with a larger volume of liquid.
Primary: Delicious Beer
Secondary: Delicious Beer, Delicious Cider
Kegeratin': Delicious Draft Beer
Bottled: Delicious Bottled Beer and Cider


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March 27, 2015
GALESBURG — Knoxville High School won the fourth annual Carl Sandburg College Welding Competition on Friday afternoon at the Center for Manufacturing Excellence on the Main Campus in Galesburg.
Excellence, Collaboration, Integrity, Respect.
Carl Sandburg College is committed to helping our students complete their college education. The College provides more than 300 scholarships annually and assists students in receiving federal, state and institutional financial aid to help them meet their educational costs.
Since 1971, more than 9,000 students have received financial assistance from Carl Sandburg College Foundation to attend Carl Sandburg College. The Foundation provides numerous academic, program-specific, general and need-based scholarships to Sandburg students. All scholarship awards are based on individual scholarship guidelines. Scholarship recipients must have a minimum 2.0 GPA in order to apply for a scholarship, and higher GPA standards are required for some scholarships. The majority of scholarships require that the recipient also be a full-time student. Applications are due by February 15 for the coming academic year.
The Carl Sandburg College Foundation scholarship application process is paperless and completely online. Paper applications will no longer be accepted unless a special application is required per scholarship requirements. To access the scholarship application website, click on the link below.
STARS Scholarship Application process is open from November 1st until February 15th each academic year, allowing students to apply for scholarships to be awarded the Fall of the following academic year. All completed scholarship applications must be submitted by February 15th. Visit STARS.sandburg.edu to complete scholarship applications.
The Financial Aid Office assists students in applying for federal, state and institutional funds to help them meet their educational costs. Most of the funding is based on the family's financial situation; therefore, the student must complete a Free Application for Federal Student Aid (FAFSA) to determine eligibility.
The Financial Aid Office is not allowed to discuss financial aid information with anyone other than you, the student. If you want the Financial Aid Office staff to be able to discuss your financial aid information with your parent(s), spouse or any other person(s), please complete the Information Release Form.
March 27, 2015
GALESBURG — Knoxville High School won the fourth annual Carl Sandburg College Welding Competition on Friday afternoon at the Center for Manufacturing Excellence on the Main Campus in Galesburg.
March 26, 2015
GALESBURG — Cam Wernentin (Bettendorf, Iowa/Bettendorf) took a perfect game into the sixth inning of Game 2 and finished with a two-hit shutout as the Carl Sandburg College baseball team split a doubleheader...
March 26, 2015
GALESBURG — The 24th annual Carl Sandburg College Career Expo will take place from 11 a.m. to 1 p.m. April 7 in John M. Lewis Gymnasium on the Main Campus in Galesburg, 2400 Tom L. Wilson Blvd.
March 26, 2015
GALESBURG — The Carl Sandburg College softball team's Arrowhead Conference doubleheader scheduled for this afternoon at Kishwaukee has been postponed to 3 p.m. Monday because of unplayable conditions.
March 25, 2015
GALESBURG — Third-ranked Southeastern (Iowa) opened the scoring with a nine-run third inning and held the Carl Sandburg College baseball team without a hit as it defeated the Chargers 13-1 in seven innings...


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Featured Package:
Earl Klugh's, second annual Weekend of Jazz at Kiawah Island Golf Resort
November 10-12, 2011
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Tag Archives: digestive aid
Jun 11
We are all guilty of it… the uncomfortable, about-to-burst sensation that develops deep in your gut after over-indulging at our favorite restaurants. I am here to introduce you to a little post-meal cure … I give you Underberg.


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Can someone describe their Square leaf for me?
I got a sp. Square leaf, and it arrived dehydrated. Or so I thought. Can someone describe theirs for me? I thought the leaves were supposed to be rigid. Mine is looking pretty good now, but the leaves are still wrinkly and soft, very flexible. The potting media is coco husk chips and coco husk fiber, and doesn't seem to be drying out very quickly at all. But the plant still seems rooted. I don't know what's up with it. I'm trying to decide if it's unhappy or doing alright. I'm leaning towards doing alright, but the leaves kinda worry me.
My plant has very rigid leaves with raised wrinkle like veins. I have had my plant for close to a year and it has only grew one new vine with three pairs of leaves. When I went to take a look at the leaves I noticed that it just started new growth at two of the nodes on that new vine. I did notice that growth was faster once I moved my plant to bright light after Xuan mentioned that is how she grows her plant.
Ah, thanks for the descriptions. So it is still dehydrated. I feel like it is a root issue, perhaps. If I didn't know better, I would say it resembles a hoya cutting that hasn't rooted yet. Except that I definitely know it has roots, because I saw them, and when I tug at it gently it does not budge. It is holding its leaves upright at least. From afar it looks great. It is just when I feel the leaves that it feels wrong. Well, we'll see how it goes. It has the start of new growth that is thinking about doing something.
Hmmm. I've given it a little Physan 20 in case there's any root rot issue going on, and I tried soaking some of the leaves/stem today - one branch of it is long enough to dip into a plant saucer of water with a bit of Superthrive in it. It soaked for maybe 10-12 hours, but I really didn't notice any difference at all. I was hoping to get that bit to firm up, and maybe take a cutting of it so that I have a better chance of getting one to survive. No go, didn't work. Well, again, we'll see. I might try taking that cutting anyway.
Not better today at all. In fact, maybe a little worse. I bit the bullet and took a cutting that was basically about half the plant. Put rooting gel on the the end and below the first and second sets of leaves, and potted it so that both those spots are under some potting media (in this case, coco husk chips soaked in VF-11). It's going into the terrarium where my other smaller hoya cuttings reside. If that doesn't do it, I don't know what will. The rest of it I will not do anything special, just what I've already been doing. I want to give that a chance to see if that little bit of new growth will actually do something. If it goes downhill, I'll start that one over also, and the other cutting is already around as backup. Hope something works. I don't want to lose it.


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Know why cattywampus was trending last week?
bipolar disorder n.
An affective disorder marked by alternating periods of euphoria and depression.
|bipolar disorder
Any of several mood disorders usually characterized by periods of depression that alternate with mania.
Also known as manic-depressive illness. This psychiatric disorder is marked by periods of euphoric (manic) highs often followed by periods of depression.


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Album Summary
- Oliver Widmer (Baritone)
- Isabel Rey (Soprano)
- Ruggero Raimondi (Bass)
- Juan Fl�rez (Tenor)
Notes & Reviews:
This is a BLU-RAY disc that will not play in a standard DVD player. A standard DVD is available as DEC B000944109. Juan Diego Florez, the king of 'bel canto' tenors, gives a truly charming performance as the lovelorn Ernesto - a role he has triumphed in at The Metropolitan Opera and Royal Opera, Covent Garden.Notes & Reviews:
Region: All
Picture Format: Blu-Ray, 16:9, Color
Subtitles: English, French, German, Italian Spanish, Chinese
ReviewsThere are currently no reviews, be the first one!
Also Purchased
Works DetailsDonizetti, Gaetano : Don Pasquale
- Performers: Oliver Widmer (Baritone); Isabel Rey (Soprano); Ruggero Raimondi (Bass); Juan Flórez (Tenor)
- Conductor: Nello Santi
- Ensemble: Zurich Opera Orchestra
- Period Time: Romantic
- Form: Opera/Operetta
- Written: 1843


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BAE Systems Barrow could clinch gun deal
Last updated at 17:22, Friday, 15 January 2010
BARROW'S BAE armaments plant could clinch orders for 145 more M777 ultra light army field guns.
There has been growing expectation that India will buy the Barrow designed howitzers.
The howitzers are manufactured by BAE jointly in Britain and America and cost more than £1m each.
Now a top Indian newspaper claims the Indian Government has authorised an outright purchase of 145 of the howitzers from the US.
The order would mean at least a year's work for the BAE Global Combat Systems M777 project workers in Barrow – who build central complex parts of the gun out of lighter titanium and aluminium alloys.
A union source said: "It would mean at least an extra 12 months to 14 months work for Barrow which would be great news."
But he said the plant would remain cautious until an order was actually placed.
Currently 862 of the guns have been ordered by US forces, and 38 by Canada. Australia is also expected to order around 35 guns.
The number of howitzers ordered will go over 1,000 if the Indian order comes to fruition. The ultra-light howitzers are wanted by the mountain artillery divisions of the Indian Army to be used in high-altitude frontiers opposite Pakistan and China.
They can be transported slung from some helicopters.
The Calcutta Telegraph says the country's defence acquisitions committee has decided to take the foreign military sales route.
But no order has been placed.
The BAE gun is competing with another firm for the order but is thought to be favourite.
The army reportedly wants to buy 145 ultra-light howitzers, 158 towed and wheeled, 100 tracked, and 180 wheeled and armoured guns in the first phase as part of its field artillery rationalisation plan, the programme to upgrade its artillery divisions.
About 400 people work at the arms plant in Barrow working on the M777 and other projects.
But the plant is in the process of shedding around 60 jobs because of a rundown in other work.
BAE declined to comment on the possible Indian order which it said was only speculation.
First published at 12:59, Friday, 15 January 2010
Published by http://www.nwemail.co.uk
Have your say
Some rare good economic news in Labour's recession, but voters in Barrow must vote Labour to block a Tory Commons majority. Electors can safely vote Labour there to stop it without Brown keeping his own majority, whereas further up the Tory target list in Northallerton voting Labour is too risky.  A win there probably secures another Labour majority so in Northallerton the Tories must be backed to stop them.


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Reader's view: How was Vietnam service relevant to drug story?
Can someone at the News Tribune please explain to me as an honorably discharged, in-country, U.S. Marine Corps veteran of the war in Vietnam how two men being "Vietnam veterans" had anything to do with their illegal activities described in the May 10 story, "Local Vietnam vets admit transporting pot across South Dakota"?
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Welcome to Homegirls. You'll find a sassy sampling of décor and design tips, frank conversation about everything from holidays and homekeeping to home improvement and our picks and pans of new products, stores and events.
Contributors: Kim Palmer, Lynn Underwood, Connie Nelson, Kim Ode and Nicole Hvidsten.
Email us with tips or questions.
To read Greengirls posts, go here.
Home improvement projects can simmer for years on the back burner, but eventually they have a pesky way of bringing themselves to the fore and making you deal with them.
Take the recent closet avalanche before work one morning. An attempt to find a particular pair of running pants brought down the house of cards that was the storage system for my husband's workout clothes. It was the perfect storm of too much accumulated stuff and an inefficient storage setup we had tolerated since moving in. A sudden freefall of clothing yielded a certain amount of swearing on someone's part, an enormous mound of clothes heaped on the bed, but no sign of the elusive pants.
I seized the moment to suggest a swing by Ikea on the way home to check out more useful storage options. An hour of shopping, $63 and a certain amount of assembly that involved some satisfying whacking with a rubber mallet later and we had the bare bones of a workable closet system. Not the closet system of our dreams or the stuff of magazine spreads, mind you, but serviceable and a vast improvement.
While the new set of pull-out bins was more accommodating than the old shelving, we still couldn't put away quite all of the pile, but some much-needed purging of items not worn in years solved that problem. And then we were left to ask that age-old homeowner's lament: Why didn't we do this sooner?
How about you? What home improvement projects have made you wonder why you waited so long? And how have you tamed closet catastrophes?
Oh, and those elusive pants? They were eventually found in the laundry basket.
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Bringing engineering to life across North Carolina through outreach, mentoring and education
The NC State College of Engineering offers week-long summer day camps for elementary school students in Raleigh and partner locations: Charlotte, Hickory, Morganton and Rocky Mount. Campers learn how engineers impact society, and they experience engineering themselves through hands-on, creative investigations and design activities. New activities will be introduced in 2015. Past activities have involved rocket design, egg drops, lip balm, silly putty, and more.
Who: Students entering 3rd through 5th grade in Fall 2015.
What: A week-long day camp filled with fun engineering activities.
Where: All over North Carolina!
When: June through July. The dates vary by location.
Why: Because it's fun!
How: Check individual locations for application information.
How much: $250 - $375. Fees vary by location. Financial aid may be available for all families that demonstrate financial need.
The online application for all Raleigh summer camps opens January 5, 2015. It will remain open until April 1, 2015.
Select the red LOGIN/APPLY NOW button above to open the application portal, which has a new look this year.
First read Summer Programs Questions & Answers.
Still need help? Contact the Summer Programs Staff.


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Rollin' on the River
Columbia River Pilots-- River Tales, History & Commerce
From Library Staff
An illustrated book of the commerce along the river, historically and currently. Interesting reading for anyone who has viewed with curiosity the myriad barges and commercial ships, domestic and foreign, that have passed by enroute to ports at sea or inland.
From the critics
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Celebrity Central / Top 25 Celebs
Robert Pattinson
Which Couple Are You Looking Out for at the Met Gala?
Lea Michele & Cory Monteith and Lindsey Vonn & Tiger Woods are also set to attend Monday's event
Kristen Stewart in Fender Bender in L.A.
The actress is uninjured after bumping into another car on the road
Coachella: Relive the Music Festival in Six-Second Clips
From pool parties to carnivals, see how festival-goers cool off during the annual desert festival
Kristen Stewart and Robert Pattinson Stay Close at Coachella
The couple were snapped holding hands as they made their way through the festival crowd
Rob Pattinson Celebrates Kristen Stewart's Birthday with Pals
The couple have a low-key Mexican fiesta for the actress's 23rd birthday
Robert Pattinson & Vince Vaughn Step Out Together in Hollywood
The actors dined together on a special guy's night out in Hollywood
Robert Pattinson and Kristen Stewart Cuddle over Lunch
The reunited twosome are spotted having lunch with friends in Los Feliz, Calif.
Robert Pattinson and Kristen Stewart Celebrate a Birthday
In a six-second video, Perry leads the table in singing "Happy Birthday" to her assistant
Robert Pattinson and Kristen Stewart Reunite After 2 Months Apart
The Twilight stars, rocked by a cheating scandal, were apart for two months
Jennifer Lawrence Gets Five MTV Movie Nominations
But Breaking Dawn – Part 2, which has won best kiss in the past, gets just one nod for Taylor Lautner
Breaking Dawn – Part 2 Sweeps the Razzies
The finale to the vampire saga "wins" seven, as Adam Sandler and Rihanna are also "honored"
Liberty Ross Files for Divorce from Rupert Sanders
Ross is reportedly seeking joint custody of her two children with Rupert Sanders
Is Robert Pattinson Rehearsing for The Bachelor?
At an afterparty for the Golden Globes, the Twilight star gives Tina Fey a rose


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Radiant Vitality Energy Lotion 200ml
Item No.1230100099
This high-performance, energizing treatment lotion with anti-oxidants deeply hydrates skin. Rich, cushioning lotion infuses skin with comforting moisture for a look of radiant vitality. From the Nutritious Collection, powered by pomegranate. See a new look of active, radiant health.
This item is available for
UK Home Delivery Free (UK orders over £30) or £3.99
Delivered within 4 working days
Next or Named Day Delivery
7 days a week between 8am - 6pm
Monday to Friday - order by 7pm - £4.99
Saturday & Sunday - order by 6pm - £4.99
Next or Named Evening Delivery
7 days a week between 6pm - 10pm
Monday to Sunday - order by 10pm - £6.99
Collect from store the next day - FREE
Order before 7pm for next day collection after 12pm midday
Show participating stores
Full Delivery details
- Free gifts are while stocks last. Gift is out of stock if not added to bag & NOT valid if used with a discount promo code


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But it doesn't have to be.
Continue Shopping.
iHerb Customer Reviews
Very good product
5 people found the following review helpful
Posted by FEED-ME-MORE on Oct 17, 2012
All the family enjoys benefits from this product because it is indispensable, especially if you live in country/place in which you can't get enough normal fish, sea products with your diet. And as winter season is coming it is also even more needed to consume.
Great product!
5 people found the following review helpful
Posted by chenpaoyu on Jul 03, 2012
I have been taking omega-3 fish oil for more than ten years. I must say that this is a major supplement I cannot live without. My neck used to pain when I turn my head aside. After taking fish oil, I feel no pain in my neck. It is true. I start with taking one softgel three times per day. Now I take one softgel only per day for daily maintenance. I used to eat fish oil from New Zealand. This is the first time I try Madre Labs. The quality seems not worse than New Zealand product, and the price is right. I believe I will keep taking Madre Labs fish oil.
Overall Good
3 people found the following review helpful
Posted by Reviewer2926152 on Dec 02, 2011
Shipped: Came extremely quick (2-3 days) Smell: Little fishy if you smell the bottle, but that is to be expected. No smell after gel pill is swallowed. Price: Great price, especially if you can get free shipping!
Супер цена!
3 people found the following review helpful
Posted by Novella on Oct 24, 2013
Оптимальное соотношение цены и качества! Беру всегда!
Good stuff
8 people found the following review helpful
Posted by Reviewer2081675 on Feb 22, 2012
From the first weeks of consistent use I felt a considerable relief from a very bad case of PMS (breast pain, depression, etc) that nothing else relieved before. I'll buy again for sure. Z from PR


text:
PC - Windows
Steam Achievements for Nuclear DawnHome / PC - Windows /
This page contains Nuclear Dawn Unlockables for PC - Windows called "Steam Achievements" and has been posted or updated on Oct 15, 2011 by Lonewolf1991. Let us know what you think of this Unlockable by thumbs voting.
Boxshot / Details
- Developer: Interwave Studios
- Publisher: Viva Media, LLC
- Genre: Action Shooter First-Person
- Release Date: Sep 22, 2011
- ESRB: Mature
Steam Achievements
To unlock the following, you have to play the game and collect/Earn achievements. You can find those earned achievements in your Game-Mainmenu or you can view them if you signup at one of the Steam communities.
|How to Unlock:
|Angel of Death
|Score 25 kills in a row without dying.
|Armour Feels Good
|Spawn as an Exo soldier 50 times in a row.
|Around The Track
|Score a kill with every class and weapon kit in the game.
|Big Brother
|As a Commander, heal at least 4 of your troops with a single healing ability.
|Big Game Hunter
|Kill 20 Exo soldiers.
|Score 5 kills in a row without dying.
|Charging In
|Sprint for a total of 20 kilometers.
|Cloak-on-Cloak Action
|Get killed by a Stealth's melee while you are cloaked.
|Colossal Stealth
|Kill a Commander at their console in their Commander Bunker, while playing as Exo infantry.
|Come Out And Play
|Kill 20 cloaked Stealth infantry.
|Kill yourself by firing at the ground with a siege weapon.
|Cresus Was Here
|Spend at least x resources in a single game.
|Cyber Ressurection
|As an Engineer, repair a structure that was at 10% of its hit points or less.
|Win a Warfare game without dying.
|Deep Blue
|Unlock every single research item within a single game.
|Full House
|Destroy one of each structure.
|Golden Targets
|Kill 3 Commanders in the field, out of their bunkers.
|Score a headshot with every bullet weapon in the game.
|Hospital Duty
|Heal to a total of at least 10,000 hit points with a single Supply Station.
|Huffing And Puffing
|Destroy 5 bunkers.
|I Don't Like Bunnies
|Land a headshot on an enemy while they are airborne.
|I Moisturize
|Get healed by 200% of your health in a single game without dying.
|I'll Fix You All
|Spawn as a Support infantry 100 times in a row.
|I'm Just Shy...
|Backstab 5 players without dying.
|Industrial Revolution
|Build one assembler.
|It's Alive
|As a Medic, heal a player that had 10 or fewer hit points.
|Leading The Troops
|Win a warfare game as an RTS Commander.
|Life, Interrupted
|Kill a player while they are being healed.
|Man Versus Machine
|Destroy 20 turrets.
|Master Commander
|Win 10 warfare games as an rts commander.
|Build 250 turrets, of any type.
|Poke In The Eye
|Build 20 Radar structures.
|Property Damage
|Inflict 100,000 points of damage on structures.
|Put The Tool Back In Your Pants
|Kill 5 Engineers as they repair a structure.
|Running Up
|Run a total of 10 kilometers.
|Semper Assault
|Spawn as an Assault soldier 100 times in a row.
|Sneak Kill
|Kill a Commander at their console in their Command Bunker.
|Sniping With Love
|Score 3 kills with a sniper without dying or missing a single shot.
|Some Like It In The Shadows
|Spawn as a Stealth infantry 100 times in a row.
|Spread The Pain
|Inflict 5,000 points of damage on players.
|Team Domination
|Capture all resource points in a map.
|That's The Way You Hold Them
|Successfully backstab and kill an enemy with a single blow.
|The More The Merrier
|Build 5 spawn gates.
|The Smell of Victory In The Morning
|Win a warfare game as an FPS soldier.
|There's Plenty To Go Around
|Kill three players with the same explosive weapon.
|Theresa, Mother Theresa
|As a Medic, heal at least 10 people with the same medpack.
|Use Candels
|Destroy 50 secondary power structures.
|Veteran Trooper
|Win 25 warfare games as an FPS soldier.
All Unlockables
Back to all Cheats
From: Lonewolf1991
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|<< First < Previous 1 2 Next > Last >>


text:
The Spring Resort & Spa
No availability found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this resort rated in the past?
- Swimming pool
- Free High-Speed Internet
- Kitchenette
- Free parking
Travelers also recommend:
- More recommendations
Historical Traveler Reviews The Spring Resort & Spa Desert Hot Springs
Luxurious, Private Paradise
OUTSTANDING!! This luxurious, private hotel and spa is exceptionally refreshing. From the moment you walk in you get a calm feeling that rushes over you. Barbara will greet you with a glass of water and show you around. There are only 9 rooms. The rooms have a bedroom and a kitchen. The sheets and bed are so comfortable it is hard to get out of bed. The sheets must be 600 thread count. Each room has a new gas stove, pots, pans, plates, glasses, bowls, silverwear, microwave, fridge,can and bottle openers. In the bedroom there is not only one of the most comfortable beds I have ever been in but they provide you with a small alarm clock and a stereo with a cd player and remote. The natural hot springs (there are 3 of them) come up out of the ground at 170 degrees. There is a hot tub (the hottest) then the soaking pool which is enclosed in a gazebo (the next warmest) and of course the pool (very refreshing) Each room is poolside and you can be in the water 24hours a day. There is true dry sauna open till 11pm. Robes are provided to each guest. You will not find a better staff. They are there to pamper you every need. The property is extremely well kept and the spa services are as 5 star as the rooms and hot springs. If you are going to the desert there is no better place to stay. I would highly recommend The Spring to anyone looking for a getaway!!!
Feel free to email me with any questions!!
The Ulimate Place to Relax
The Spring Resort and Spa has a remarkable attention to detail. From every angle the Spring relieves stress. My Wife and I spent 2 glorious nights and cannot wait to return. There is no other place to stay for relaxion in the Palm Springs area.
The best that the desert has to offer.
For peace and relaxation this place can't be beat.
This is the place to get away from it all in the desert without having to sacrifice any creature comforts.
The perfect place to unwind
The perfect place to unwind when have been stressed out to the maximum. Very nice setting, great pools, and with only nine rooms I would say quiet and calm best describe it. I had one of the best massages that I have ever had anywhere.
Peaceful and relaxing
My wife and I spent 3 nights at the Springs. We decided to get a kitchen and cook all our meals rather than having to get dressed and go to a restaurant. All we wore were 2 swim suites the whole time. We were so relaxed and at peace that we didn't even take advantage of the spa service. I love relaxing in water that feels absolutely fresh.
Just one negative. If you plan on any real cooking, bring a larger pan and a baking dish. Our hosts provided what we needed and we had a wonderful time.
The Ultimate Getaway
The Spring is the ultimate romantic getaway! We celebrated our anniversary there. Nine rooms surround a pool fed by natural hot mineral springs. Soft music and a bubbling fountain are the only sounds that break the serenity. The rooms have no telephones or televisions -- just bring a book, nap, soak in either an indoor or outdoor hot tub, dip in the pool or have a massage -- or never leave your room. My wife loved it! Located high up above the valley, you can look across at the snow covered mountains while swimming in the pool. What a trip! Pure relaxation! I recommend it highly.
That much needed quiet getaway
I have always been looking for that quiet place where I can be pampered and return home rejuvenated without going away for more than a a day or two. This is the place. I had a lovely room with a sumptuously comfortable bed overlooking a quiet pool. A beautiful indoor hot spring pool as well as one under the stars. Very quiet, only nine rooms, making it amazingly restful. A gifted spa staff that made by body feel like new. I will be spending a lot of time there.
Remote but relaxing
My fiancee and I spent Valentine's Day weekend at The Spring. It took almost 3 hours to get from San Diego to Desert Hot Springs, but it was worth it. Upon our arrival we were surprised to find that the rooms have no televisions, only radios. There is a quiet living room with a fireplace that guests can sit in and relax. Clearly, the very best part of the The Spring, are the springs. The water is warm and cozy, and since there are only 9 rooms availble at this resort, you rarely share the water with anyone. I received a Clay wrap with massage combination, and it was wonderful! My fiancee received a 90 minute massage that he wished had gone the whole day. Close by is just about the biggest outlet sector I've ever been to, which was also fun. The best part of our trip was our dinner in La Quinta at The Cliffhouse. HIGHLY RECOMMEND THIS RESTAURANT. Although it took about 25 minutes to get to the restaurant, it was well worth it. Lobster Bisque is a must! My one and very only complaint is that the resort is very remote...far from any attractions Palm Springs has to offer. Next time I'm pretty sure we will try to stay more in the city of Palm Springs...nonetheless, we would absolutely return to The Spring...it was truly relaxing and we had a hard time leaving.
Peace, Rejuvenation & Luxury
I've been to The Spring three times and each time have come away vowing to return soon. The water is extraordinary... as are the massage therapists. The place has tremendous serenity. The beds are outstanding and the atmosphere is peaceful... from the colors, to the music, to the views. I felt well-taken care of. My strongest memory of The Spring is just appreciating every minute.
Rejuvenation at The Spring
I recently spent a great weekend at "The Spring Resort & Spa" in Desert Hot Springs, California. It is a small (9 rooms), very private resort with beautifully tailored rooms with plush down comforters, pillows etc. It sits on one of the word's greatest Natural Hot Springs, so the water is very comforting and relaxing. I had a great massage and a great facial. They offer great Spa Packages as well. I highly recommend this oasis in the desert.
TripAdvisor Reviews The Spring Resort & Spa Desert Hot Springs
Travel Blogs from Desert Hot Springs
Ready to Ride Again. Let's Hit the Road!
... two-wheeling, and approaches riding as you would expect from the mind of an analytical engineer.
Ray is a positive, steadying influence on me. Every time we ride together, I learn something new. About riding, about Ray, and about myself.
Beginning tomorrow, each evening after we park the bikes for the night, you will receive a short e-mail notification with a highlight or two of the day's ride, and ...
Desirable Pueblo - 14 Up, 31 Down
... br>
A few days hanging around waiting for overnight temperatures in Mesa Verde – which is up to 4000 ft higher than Moab - to stabilise above freezing then we can leave Moab.
Mesa Verde (spanish for green table) is not strictly a mesa, but rather a questa as it has 7 deg slope ...
The Good Life
... desert! We pulled in and while Bob registered I walked Nibs. Of course he wanted to be with Bob and he dragged me toward the office door. The ladies inside all made a fuss over him and I was told that, "of course" I could bring him inside the office.
We were assigned spot #472 which was at the end of a row and was a pie shaped lot backing onto Bubbling Wells Road. The front of the ...
Thousand Palms Oasis Preserve
... the floor of which is littered with large piles of fronds.Its really impossible to tell which trees these fronds came from as all the trees have dead fronds still attached from base to top. Trees grow so close to one another that no sunlight reaches the earth on which they grow and it is cold walking amongst them.
Pushing on down the trail we come to a board walk which keeps our feet from getting wet. Many of the palms have their feet ...
Visiting The Whitewater Preserve
... br> The Whitewater Preserve is 2868 acres surrounded by the Bureau of Land Management San Gorgonio Wilderness. It is named for Whitewater River that runs through it. This place used to be a privately owned trout farm until 1995 when it was purchased by the Wildlands Conservancy. The ranger station is a large open building that is ideal for holding meetings. The folks behind the counter were extremely informative and people friendly. We asked if ...


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text:
I asked this question before, but had no replies, maybe I wasn't so clear.
I'm trying to debug a django project using MySQL database. If I run the admin or trying to use the shell to communicate to the data base every thing is well and I can do every thing. I installed MySQLdb for Python 2.6.
I installed PyDev on my Apatana studio. Configured the Debugging with runserver 8001 --noreload.
When I start debugging , When I arrive to the following code in C:\Python26\Lib\site-packages\django\db\backends\mysql\base.py
import MySQLdb as Database
except ImportError, e:
from django.core.exceptions import ImproperlyConfigured
raise ImproperlyConfigured("Error loading MySQLdb module: %s" % e)
I get an import error : django.core.exceptions.ImproperlyConfigured: Error loading MySQLdb module: DLL load failed: The specified module could not be found.
I trying to ge more deeply with the import MySQLdb as Database line , it goes to the C:\Python26\Lib\site-packages\MySQLdb__init__.py and fail in the line import _mysql.
I can't understand the problem. When running the Django admin every thing is ok, but with debugging it fails to work.
Any help please. Thanks in advance.


text:
Disclosed is a method for the extraction of lipase from mammalian tissue with which it is associated which involves contacting the tissue with an alkaline, aqueous medium having a pH of from greater than 7.0 up to a level at which the alkalinity will deactivate the lipase.
1. A liquid extract of calf, kid or lamb pregastric lipase having a pH greater than 8.0 but lower than the pH at which the lipase is deactivated.
2. The extract of claim 1 wherein the lipase is from a calf and the pH is no greater than 11.0 or the lipase is from a kid or a lamb and the pH is no greater than 8.5.
This is a divisional of copending application(s) Ser. No. 07/567,630 filed Aug. 15, 1990, now U.S. Pat. No. 5,320,959, issued Jun. 14, 1994.
The commercial application of lipases (esterases) from animal origin are varied. For example, these enzymes find utility in natural flavor production such as in enzymatic modification of lipid food ingredients and in cheese ripening processes by shortening the cheese aging time. The lipases are also useful for the formation of enzyme modified cheese flavor in the production of certain Italian type cheeses such as provolone. They also find utility in butter fat modification, for developing products which are useful in bakery/cereal products, imitation dairy products, margarines, salad dressings and pizza sauces.
The development of flavor in cheese under storage conditions is attributed to a combination of breakdown of products from milk constituents, mostly from fat. The presence of free butyric acid, caproic acid and capric acids in aged cheese is largely due to the action of lipases on cheese fat. Thus, the cheese-ripening time and the flavor profile can be altered by the amount of available lipase in the cheese. Lipases have been extensively used in the production of enzyme modified cheese (EMC). Lipolysed milk fat flavored dairy products are produced by incubation of a pregastric lipase with butter fat or vegetable oils. The resulting hydrolysate is then used as a flavor booster in food products.
Animal lipases have been historically used to enhance the flavor in non-dairy products. The increasing demand for animal lipases in food and dairy industries necessitates the development of an improved processing technology for obtaining higher yields from The limited supply of animal sources.
Animal lipases are typically obtained by drying and grinding calf, kid or lamb gullets to provide a solid, enzyme containing product in which the enzyme is closely associated with the gullet tissue. When used, such as in the making of enzyme modified cheese, the ground gullet bearing the enzyme is added to the vat and blended into the cheese milk. This method suffers from the disadvantage of providing pockets of lipase activity due to the incomplete mixing of the solid lipase containing gullet tissue and the liquid cheese milk. In U.S. Pat. No. 2,531,329 there is disclosed a method for extracting lipase from animal tissue (gullet) using an aqueous solution containing 10% propylene glycol together with sodium chloride at an acidic pH (pH 6.2-6.5).
The advantages of a liquid form of animal lipase are apparent, and it is an object of the present invention to provide such lipase in the liquid form as well as a method for its preparation. A further object is to provide such a method which provides for the extraction of lipase using an alkaline, aqueous medium which does not require additives to enhance extractions by the aqueous medium.
The present invention is a method of extracting lipase from the tissues of meals with which it is associated which method comprises contacting the tissue with an aqueous alkaline media having a pH of from greater than 7.0 up to the pH level at which the lipase is inactivated for a time sufficient to cause the dissociation of the lipase from the animal tissue and provide an aqueous solution containing the lipase activity. The aqueous solution is then separated from the remaining animal tissue by solid/liquid separatory techniques. Also included within the scope of the present invention is the aqueous solution of animal lipase.
Conventional sources of animal lipases include the gullets from calf, kid or lamb, although any milk weaned mammal can be regarded as a suitable source. The lipase from these sources is known as pregastric lipase. All meals, including man, produce these lipases due to the need to break down complex dietary lipids. These lipases remain firmly bound to the gullet tissue when placed in acidic or neutral solutions in the absence of salt and other extractants such as ethylene glycol or propyleneglycol, however, it has now been discovered that lipase can be isolated by extraction using an aqueous alkaline medium. A medium with a pH even nominally above 7.0 is satisfactory for the extraction although a pH of at least 8.0-8.5 is preferred to achieve an extract having maximum stability. Care must be taken to avoid too high a pH since undue alkalinity will inactivate the lipase. The maximum pH at which the extraction can be carried out will vary depending on the animal tissue which is being worked with. Thus, in the case of kid or lamb lipase, the extraction should be carried out at a pH of no more than 8.5 whereas in the case of calf tissue, a maximum pH of up to 11.0 can be employed without causing the enzyme's inactivation. The maximum pH which can be used to extract lipase from other mammals such as rabbits, monkeys or pigs will vary but can be readily determined without undue experimentation.
The extraction is typically carried out using finely ground, wet or dried gullet tissue which is added to the alkaline extraction media, usually with stirring, at a temperature preferably within the range of from 5� to 30� C. The extraction time will vary depending on the particular conditions of extraction, but will typically range from 1/2 hour to 10 hours. After the extraction is complete, the animal tissue is separated from the lipase solution by liquid/solid separatory techniques such as filtration or centrifugation with decantation to provide a clear enzyme solution. This solution can either be used as is, or it can be further modified such as by drying to produce a powdered product before being added to a system in which the lipase activity is required. A particularly desirable lipase formulation will contain from 20 to 100 lipase forestomach units per gram (LFU/g). The lipase activity is determined by measuring the free butyric acid liberated from tribytryin substrate using a pH-stat under standard conditions, i.e. pH 6.2, 42� C. as described in FCC 111/General Tests and Apparatus/493. One LFU is the activity that releases 1.25 μ mol of butyric acid per minute under the conditions of the assay. Activity of the enzyme preparation is calculated by the following formula: ##EQU1## where W is the weight, in grams, of the enzyme preparation contained in the 1.0 ml of sample preparation taken for analysis, R is titrant delivery rate in ml/min., and 0.025 is the normality of the titrant.
The above described assay technique was used in the following examples which further illustrate the method of practicing the present invention and the results achieved thereby.
Frozen gullets of calf, lamb and kid were thawed and ground in a meat grinder. One hundred grams of ground lamb gullet was suspended in 1.0 liter of distilled water and the suspension homogenized for five minutes using a mechanical homogenizer. Aliquots (100 ml) were placed in individual beakers and the pH was adjusted to pH 5.0, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0 respectively. Samples were placed in an ice bath and stirred for 60 minutes.
A portion of the suspension was then centrifuged to separate the insolubles (tissue), and the clear supernatant was tested for enzyme activity. Lipase activity was determined for corresponding supernatant and non-centrifuged samples by autotitration methods. The percentage activity appearing in the supernatant was calculated at each pH value.
The experiment was repeated under identical conditions using ground gullet of kid and calf (except that the pH was raised incrementally to 12.0 in the case of the calf tissue). The effect of pH on the extraction of lipase from the gullets is presented in Tables 1a, b and c.
TABLE 1______________________________________pH EFFECT ON EXTRACTION OF LIPASE______________________________________A. LAMBSample Non-Centrifuged Supernatant ActivityAssayed LFU/ml LFU/ml Sup/Meat______________________________________pH 5.0 9.6 0.6 6.3%pH 5.0 12.0 3.8 31.7%pH 6.0 12.8 3.8 29.7%pH 6.5 13.8 7.6 55.1%pH 7.0 16.8 8.2 48.8%pH 7.5 13.6 13.0 95.6%pH 8.0 18.2 18.2 100.0%pH 8.5 17.4 17.4 100.0%pH 9.0 5.8 4.0 69.0%______________________________________B. CALFSample Non-Centrifuged Supernatant ActivityAssayed LFU/ml LFU/ml Sup/Meat______________________________________pH 5.0 24.0 1.0 4.2%pH 5.5 24.4 2.8 11.5%pH 6.0 27.1 5.6 20.7%pH 6.5 30.6 4.8 15.7%pH 7.0 25.3 5.3 20.9%pH 7.5 26.0 17.6 29.2%pH 8.0 27.6 10.6 138.4%pH 8.5 42.4 23.8 156.1%pH 9.0 48.2 32.4 67.2%pH 9.5 44.4 37.7 84.9% pH 10.0 46.6 43.2 92.7% pH 10.5 46.8 47.0 100.4% pH 11.0 35.2 34.0 96.5% pH 11.5 26.0 28.6 110.0% pH 12.0 8.7 9.0 103.0%______________________________________C. KIDSample Non-Centrifuged Supernatant ActivityAssayed LFU/ml LFU/ml Bind Index*______________________________________pH 5.0 83.2 14.9 17.9%pH 5.5 102.2 60.5 59.2%pH 6.0 97.0 100.0 103.1%pH 6.5 180.4 113.2 62.7%pH 7.0 173.5 122.0 70.3%pH 7.5 189.0 178.5 94.4%pH 8.0 223.0 204.0 91.5%pH 8.5 275.4 289.2 105.0%pH 9.0 140.4 148.0 105.4%______________________________________ *Binding Index = % lipase solubilized
From the above data it can be determined that the separation of lipase from ground gullet is increased with increasing pH and reaches a maximum around pH 8.0-8.5. A pH greater than 8.5 caused the inactivation of the enzyme in the case of its extraction from lamb and kid tissue whereas lipase from calf tissue was not inactivated until a pH of greater than 10 was reached. More than 50% of the lipase was associated with the tissue at pH 6.0 and binding of lipase with tissue was increased with decreasing pH. It is assumed that the increase in the negative charge of the lipase in the alkaline pH may have caused either the dissociation of aggregated lipase molecules or separation of tissue-bound lipase. In any case, alkaline pH treatment of ground gullet separated the tissue-bound lipase, resulting in a marked increase in the recovery of the enzyme.
Commercially available lipase product is basically a dry gullet powder. The effect of pH on the extraction of lipase from this powder product was studied. A 2.5% (2.5 g/100 ml) suspension of the powder in water was made and the pH of the suspension adjusted to various pHs using alkali (0.2N). The suspension was then stirred at 5� C. for one hour. The solubilized lipase was separated by centrifugation. The effect of pH on the lipase extraction from lipase product was determined by measuring the lipase activity before and after centrifugation. The data presented in Table 2 clearly demonstrate a dramatic effect of pH on the dissociation of tissue bound lipase.
TABLE 2______________________________________EFFECT OF pH ON THE EXTRACTION OF LIPASEFROM POWDERED LIPASE PRODUCTEXTRACTION NON-FILTERED FILTEREDpH LFU/ml LFU/ml______________________________________A. KID POWDERpH 5.0 12.5 6.0pH 5.5 15.6 10.4pH 6.0 19.6 12.4pH 6.5 24.0 17.2pH 7.0 25.6 21.7pH 7.5 24.8 23.6pH 8.0 24.4 24.4pH 8.5 24.4 24.8pH 9.0 23.8 18.8B. CALF POWDERpH 5.0 6.2 0.1pH 5.5 7.5 0.6pH 6.0 8.7 1.0pH 6.5 7.5 1.6pH 7.0 8.0 2.9pH 7.5 9.0 3.9pH 8.0 8.4 4.2pH 8.5 6.8 4.5pH 9.0 7.2 5.1C. LAMB POWDERpH 5.0 3.3 0.5pH 5.5 3.6 0.6pH 6.0 3.0 0.7pH 6.5 3.9 2.6pH 7.0 3.7 3.3pH 7.5 4.5 4.0pH 8.0 5.0 4.5pH 8.5 5.6 4.1pH 9.0 4.8 3.3______________________________________
The binding of lipase to the ground tissue was determined at two different pHs (pH 6.0 and 8.5/0.5 for calf) as described in Example 1. After alkaline extraction of the enzyme, the pH of the treated materials was decreased to pH 6.0. The suspension was stirred for another 30 minutes at 5� C. The lipase activity was determined for corresponding supernatant and non-centrifuged samples. The results of this experiment are summarized in Table 3.
TABLE 3______________________________________Effect of pH on the Extractionof Lipase from Ground Gullets______________________________________LAMBpH 6.0 pH 8.5 pH 6.0______________________________________Non-filtered Non-filtered Non-filtered(15.2 LFU/ml) (33.3 LFU/ml) (29.8 LFU/ml)Filtrate Filtrate Filtrate(2.6 LFU/ml) (29.2 LFU/ml) (14.6 LFU/ml)% Adsorbed % Adsorbed % Adsorbed(83) (12) (51)______________________________________CALFpH 6.0 pH 10:5 pH 6.0______________________________________Non-filtered Non-filtered Non-filtered(10.9 LFU/ml) (15.6 LFU/ml) (15.1 LFU/ml)Filtrate Filtrate Filtrate(1.2 LFU/ml) (16.4 LFU/ml) (1.3 LFU/ml)% Adsorbed % Adsorbed % Adsorbed(89) (0) (91.4)______________________________________KIDpH 6.0 pH 8.5 pH 6.0______________________________________Non-filtered Non-filtered Non-filtered(93.6 LFU/ml) (221.0 LFU/ml) (172.0 LFU/ml)Filtrate Filtrate Filtrate(93.2 LFU/ml) (221.0 LFU/ml) (82.8 LFU/ml)% Adsorbed % Adsorbed % Adsorbed(0) (0) (52)______________________________________
The data in Table 3 demonstrate the reversible association of lipase to tissue. In fact, the effect is more profound with calf compared to lamb and kid. Thus, by carrying out the extraction at a pH where reassociation of the enzyme and tissue do not occur, one can realize an increase in the yield of usable enzyme.
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AC/DC Nominated For AMA And Billboard Touring Awards
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