text:
_-={Day of War}=-_
Credits & Info
- Date
- 11/14/2008
- File Info
- Song
- 3 min 15 sec
- Score
- 4.09 / 5.00
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- Score:
- Rated 4.09 / 5 stars
- Plays & Downloads:
- 24,692 Plays | 3,314 Downloads
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- Genres:
- Easy Listening - Classical
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Author Comments
Rated 5 / 5 stars
That was really good.
The best song of war that I have ever heard.
Rated 5 / 5 stars
Makes me think of Wolfenstein. If they ever made a decent movie of it, this ought to be part of the main soundtrack.
Rated 5 / 5 stars
This song..is going to be spamed to my guild..many times during raiding on WoW.
Rated 5 / 5 stars
what a perfect harmony makes my heart beat, beats so fast
Rated 5 / 5 stars
O.o OMG! BRILLIANT!
Excellent composition my fellow artist! I LOVED the drums in the beginning. And the brass was done quite well! I MUST ask what kind of setup you use to compose this music (software, hardware, etc) because I am trying to get some of these same sounds into my music, particularly those DRUMS! I've needed some good drum samples for a long time. A lot of my songs have suffered for the lack of a good symphonic drum library. Check out some of my music if you're willing. Anyway....
Excellent piece!


text:
These Mickey Mouse Clubhouse baking cups come in counts of 50. Perfect for parties, birthdays, and more! They are brightly colored and feature Mickey Mouse.
" I received the balloons today. One was chocolate brown, but the other package was emerald green so I called the company and told them. They are... "

subset: commoncrawl


text:
Exercises to learn with Raspberry Pi
Make your own quiz game with Kids Ruby and your Raspberry Pi
Learn how to live code music with the Raspberry Pi and Sonic Pi 2.0!
Getting started with Scratch on Raspberry Pi
Getting started with Mathematica and the Wolfram language on Raspberry Pi
Learn how to set up a web server on your Pi and install WordPress
Learn how to configure a webcam to take time lapse images
Learn how to setup a Pi Camera to take time lapse pictures
Generate fairy tales and other imaginative stories with basic Python.
Learn ways in which you can use the Python Picamera library
Learn how to run the Raspberry Pi demo programs
Learn Python, a tutorial for people with experience in Visual Basic
Get your first taste of programming the world of Minecraft
A Python learning tool with beginner exercises in using variables, data structures and basic control flow
All our resources are available for free under a Creative Commons licence


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Legend of Korra is awesome
By RavenVice01 13 Comments
I like the transition from Aang to Korra. The setting of Republic City kinda has a film noir mystique about it. Things are kinda of set like the America during the early 1900's where crime was rampant and the industry was somewhat booming. Korra is like the Wonder Woman of China but a bit more peppy. Toph's daughter doesn't like the Avatar's presence in Republic City but that might be the pride talking. Aang's son Tenzin is a cool character and his family is fun to watch except for his son who seems to have a few screw looses missing. Its a pity that none of the original young characters could live for the 2nd season. I only saw Katara. Sokka is apparently dead but I'm sure he had a daughter with Suki and she became the new leader of the Kyoshi Warriors. This Amon guy is a real bad ass. He knows Aang's move to cancel out someone's bending chi forever. Something tells me that Amon was a disgraced student of Aang's and his anti-bending movement is all a front for his revenge plot. Korra falls for a guy named Mako (homage to the great Mako who voiced Uncle Iroh). Mako has feelings for Korra but now he has fallen in love with this really hot and sweet rich girl. She doesn't seem like your typical rich girl who wants to change her man so he can fit in with higher society. She is actually very considerate and loving. I just hope she is not too good to be true. Mako also has an upbeat brother named Boalin but he is kinda in the friend zone with Korra. I wish him the best of luck. If there are any Avatar fans out there, I look forward to your views.


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Stop hanging around for your favourite movies as Instant Play comes to the Video Store of PlayStation Store.
The Video Store of PlayStation Store is your one-stop shop for the biggest selection of High Definition TV shows and movies on PlayStation 3. And with Instant Play, you can choose to start watching any content as soon as you start renting it – so you'll have plenty of time to watch the things you love.
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Visit eu.playstation.com/instant-play to find out more then head over to the Video Store of PlayStation Store to start enjoying your favourite programmes and movies today.
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Celebrate the launch of Bloodborne on PlayStation 4 with an incredible trip to the Japan Expo up for grabs.
Need help with your PS4 and info about the latest system software update?


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This is a discussion on Sand Blast Rally Video Clips within the Motorsports Talk forums, part of the Community - Meet other Enthusiasts category; ...
cant view !! gggrrrr ...why do we have to log in to FB to view ?...
ScoobyDMC #000,HawkEyeAlliance#000,Blobeye Syndicate #000, SQC-NY #009, "Dragon Slayer"
"All We'll Drive"
Subaru Worldwide/Rallying: facebook.com/groups/subarumad/
- Will
First Mods: What to do and what not to do
Current Car: 2014 Golf TDI
Previous Cars:2012 WRB WRX Hatch, 2004 RSX-S, 1998 Grand Prix GTP
"Isn't it amazing how smart you feel when you just aren't being stupid?"
They probably belong on youtube anyways
and the other one


text:
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Ottawa Senators Nation Episode 2
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It is July 26th 2012, and this is the second LIVE radio show from Ottawa Senators Nation! .. Please remember to "Friend" us at http://www.facebook.com/ottawa.nation and on TWITTER at @OSenatorsNation.
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Ottawa Senators Nation Episode 1
in Hockey
1) Chris Neils new contract.
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47 Senators write to Iranian leaders
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Hillary's EmailGate, Jeb 2016, GOP Senators' Letter to Iran, and Common Core
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Tonight we discuss Hillary's EmailGate, Jeb Bush's run for President, the letter to Iran from the 47 GOP Senators, common core, and more.
Karen Schoen is a teacher, and she is now educating people on the web and over the radio waves, she's been a dean, a business owner, a realtor, an insurance agent, and she ran for Fl state representative in her district in 2014.She has spoken for many groups including: Oathkeepers, Global Dispatch, several forums in her area during her campaign for Florida Representative.She writes some awesome articles on her own websites, http://karenschoen.com. , for agenda21today.com, America is my name.org. and other web publications. She hosts a blogtalk show called American Freedom Watch radio. She always defends conservative politics, especially where it relates to land and water rights, education, and economics.
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in Sports
The Boston Bruins finish up a quick two game hone stand with a Saturday matinee with the Ottawa Senators.
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Dial 929-477-4171 to discuss NY Rangers hockey with the Bleed Blue Show & the fans. The Broadway Blueshirts coming off the Stanley Cup Finals from a Season ago. What does the team have in store for the 2014-15 season? How will they fare vs the elite teams of the NHL? Will the team compete for the Metropolitan division crown? Ultimately, do the NY Rangers have what it takes to makie it back to the Stanley Cup Finals and win Lord Stanley's cup. The Bleed Blue show break down NY Rangers Hockey...the Bleed Blue way. Follow the platform on Twitter: @BleedBlueShow
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GUEST: Bill Katz, the editor of Urgent Agenda.....a look at the Clinton emails controversy and its impact on the 2016 campaign.....there are signs that many Democrats are concerned about her ability to conduct a competent campaign......the US-Iran nuclear deal and the letter that 47 GOP US Senators wrote warning Iran that the deal could be reversed by a future president......the state of the 2016 GOP campaign......it looks like a 3 way race between Bush, Walker & Rubio......and other items from the front pages.......
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Source Nation! Join us tonight for another amazing show of Sports Tuesday with Steve Manderson & Black College Sports with Edd J. Hayes. Tune in to receive all of your current as well as past Sports Highlights.
6:15 Lenny Randle
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Slicks and Sticks : Goes Behind the Glass with NHL and College Hockey
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Dr. Block, Medical Detective - Vaccines and State and Federal Legislation
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Executive Power and Fundamental Rights: Underexplored Constitutional Terrain? Thu Apr 02, 2015 06:52 | Darren O'Donovan
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Alliance Party MP and RTE's John Bowman expose Eoghan Harris distortion of Belfast flag vote and his role in Mary McAleese election
national | rights, freedoms and repression | news report Sunday January 20, 2013 22:49 by Tom Cooper
Two strikes against Eoghan Harris in this weeks Sunday Independent
Harris announced on 13 January that he, like the Sunday Independent paper edited by Anne (formerly Mrs) Harris, has been silent on loyalist flag riots in the North of Ireland. The riots started out as an attack on the moderate unionist Alliance Party. Alliance brokered a compromise on Belfast City Council that permitted flying the Union Jack flag on 18 designated days per year. The SDLP and Sinn Fein plan had been for either a shared no-flag space in the council or for parity of esteem by flying the Irish Tricolour with the Union Jack. Unionists don't do compromise.
After the first riot on the night of the vote, Alliance Party offices and the homes of representatives were systematically attacked. See:
Caption: DUP and UUP loyalist rioters begin rampage - see 'No Surrender' lady
Caption: 'Belfast Bigot' No Surrender lady becomes YouTube sensation
Caption: HItler hears about the flag coming down - 'Downfall' spoof -
Comments (3 of 3)
by Tom Cooper Sun Jan 20, 2013 23:07
No Mention of Harris embarrassment on page one
Letters to Broadcasting Authority of Ireland on refusal to consider complaint against Eoghan Harris programmeby Tom Cooper Mon Jan 21, 2013 08:47
Dear Professor Chris Morash - Chairperson, Compliance Committee, Broadcasting Authority of Ireland (BAI),
Two letters to Broadcasting Authority of Ireland on refusal to consider complaint against Eoghan Harris programme
0.6 Mb
by Jimmy Clarkson Sat Jan 26, 2013 11:39
Guess who repeated the 'piece of cloth' gaffe in the Irish Times - see apology today 26 January 2013:


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May 23rd 2014
Local Business Spotlight: Bruges Waffles and Frites
Traditional Belgian cuisine at Bruges Waffles & Frites Read More
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Earth Center Acupuncture offers a wide range of curative traditional healing services used to treat many different conditions Read More
Shop Cake Boutique on Park City, Main Street. Read More
Argenta has home theaters, audio and automatic everything for your home. Read More
The historic Bellington Manor makes Ogden a destination wedding. Read More
View art by local artists at Finch Lane Gallery Read More
Albion Fit has what you need to look hot while hitting the gym this new year. Read More


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Hotel Kennedy
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The tourists in Bologna
... head for the flea market. Bought myself a Mao hat, pair of mittens and some audio cables and a car charger for the phones. Again it was pretty surreal as I haggled with the chinese shop owner in Mandarin whilst everyone around, including him was speaking in fluent Italian.
Finally met up with everyone at the Cafe Zamboni, strangely enough situated on Via Zamboni (I tell you these Italians must spend ages deciding on the name of ...
Burials in Bologna
Yesterday, much to Joy's delight, Michael found the house where Guido Reni died, and today, thanks to a casual question to the hotel night manager, he was able to take her to the church where Il Guercino is actually buried. This is San Salvatore, and wasn't on our list of visits, but boy was it worth the walk. The night manager, Francesco, could not have been more helpful, and was delighted that someone was taking such an interest in Bologna's artists - one in a million ...
The boxing match in Bologna
The reason for this strange title, is that we have decided to send home our laundry - the cases and now stuffed with books and in danger of collapse, and so are we from humping them on and off trains! So this morning Michael made two big parcels and set off to find a post office. He found one but was told the parcels had to be in a box. Where the ###### do we find a box in Bologna??? Well, the answer turned out to be round ...
Snow, snow, quick, quick, show
... hours and a bit gazing at Reni, Guercino, Caracci, and a stack of others. Wonderful.
Michael had seen enough in about 30 minutes so went off to explore Bologna on his own. First of all it is a very historic city. You can see, without being told, that every single building has a long and fascinating history. Almost every building (and there are many) has its own architectural style. Everywhere you look, there ...
... any more food.
See this 3 minute video of the Prosciutto and Balsamic tours. Allesandro is really amazing at what he does.
https://www.youtube.com/watch?v=ykYU3Dz XRlA&list=UUdOpdcV06POgifi9PC-ct7g< br>
Before all of this, we had sampled some local restaurants on our own and also feasted. The prices were certainly right and we were happy for the recommendations from Ermias, our apt. host. ...


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Last Day to Win This Blender!
Oct 12, 2011 4:31 pm
Like us on Facebook - we might send you a blender
Remember that blender from last week? It still needs a new home, so head to our Facebook page, throw us a "like" and you'll be entered to win a high-end appliance that completes any home cook's kitchen.
What's that? High-end appliances in manly shades of blue make better smoothies? Misinformed as you may be, you're in luck. We're looking at this shiny cobalt stud as we speak, still sporting that fresh-from-the-box kitchen appliance smell. Tempting as it was, given the beginning of butternut squash soup season, we've simply admired it from afar. Won't you adopt this blender and take it for a spin? It's got 5 speeds and those big sad eyes...
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A serious sign of spring. Yes, it's spring.
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The young NYC chef makes a "simple peasant dish"
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Page 1
Loss of RhoA in neural progenitor cells causes the
disruption of adherens junctions and hyperproliferation
Kei-ichi Katayamaa, Jaime Melendezb, Jessica M. Baumanna, Jennifer R. Lesliea, Bharesh K. Chauhanc, Niza Nemkula,
Richard A. Langc, Chia-Yi Kuana, Yi Zhengb, and Yutaka Yoshidaa,1
Divisions ofaDevelopmental Biology,bExperimental Hematology and Cancer Biology, andcOphthalmology, Cincinnati Children's Hospital Medical Center,
Cincinnati, OH 45229
Edited* by Pasko Rakic, Yale University, New Haven, CT, and approved March 31, 2011 (received for review January 25, 2011)
The organization of neural progenitors in the developing mamma-
lian neuroepithelium is marked by cadherin-based adherens junc-
tions. Whereas RhoA, a founding member of the small Rho GTPase
family, has been shown to play important roles in epithelial adhe-
rens junctions, its physiological roles in neural development re-
main uncertain due to the lack of specific loss-of-function studies.
Here, we show that RhoA protein accumulates at adherens junc-
tions in the developing mouse brain and colocalizes to the cad-
herin–catenin complex. Conditional deletion of RhoA in midbrain
and forebrain neural progenitors using Wnt1-Cre and Foxg1-Cre
mice, respectively, disrupts apical adherens junctions and causes
massive dysplasia of the brain. Furthermore, RhoA-deficient neural
progenitor cells exhibit accelerated proliferation, reduction of cell-
cycle exit, and increased expression of downstream target genes of
the hedgehog pathway. Consequently, both lines of conditional
RhoA-deficient embryos exhibit expansion of neural progenitor
cells and exencephaly-like protrusions. These results demonstrate
a critical role of RhoA in the maintenance of apical adherens junc-
tions and the regulation of neural progenitor proliferation in the
developing mammalian brain.
CNS development|cell adhesion
produce the organ of predetermined size (1–3). Apart from
maintaining intercellular adhesion, adherens junctions that are
preferentially located at the ventricular surface of the neuro-
epithelium are linked to several major signaling pathways to
regulate the rate and mode of cell division in neural progenitors
(4, 5). Classic cadherins are core components of adherens
junctions. The extracellular regions of classic cadherins mediate
adhesive recognition through homophilic interactions, whereas
cytoplasmic domains associate with a set of cytoplasmic proteins
collectively called catenins. Deletion of αE-catenin in the de-
veloping nervous system results in dysregulated proliferation of
neural progenitor cells as well as disruption of adherens junc-
tions (6). Furthermore, Lgl1-knockout mice and compound
knockout mice for both Numb and Numbl also show disruption
of adherens junctions together with abnormal proliferation of
neural progenitor cells (7–9). These findings indicate that the
cadherin–catenin complex has broader functions beyond cell–cell
adhesion in mammalian neural progenitors.
Cadherin–catenin complexes interact with the actin cytoskele-
ton through α-catenin. Formation of adherens junctions is ac-
companied by profound changes in the actin cytoskeleton and
accumulation of polymerized actin at the contact area, and both
the formation and the maintenance of adherens junctions depend
on the actin cytoskeleton (10). The Rho family of small GTPases,
including RhoA, Cdc42, and Rac1, are key regulators of the actin
cytoskeleton and coordinate junction assembly, stability, and
function (11, 12). However, these functions are mainly deduced
or constitutively active small Rho GTPases. However, the con-
sequences of GTPase signaling alteration depend profoundly on
uring brain development, proliferation, differentiation, and
cell death of neural progenitor cells are tightly controlled to
cellular context and the specificity of the mutant proteins. The
recent development of a conditional gene-targeting strategy has
provided many new insights into the physiological functions of
small Rho GTPases (13). For example, although Cdc42 and Rac1
are both implicated in epithelial apical junctions, conditional
gene deletion in the telencephalon revealed that Cdc42, but not
Rac1, is indispensable for the formation of apical adherens junc-
tions in the developing brain (14–16). These findings underlie the
importance of using conditional gene deletion to ascertain the
biological functions of small Rho GTPases.
Among all small Rho GTPases, RhoA is one of the last
remaining members whose in vivo gene-deletion consequences in
the mammalian central nervous system are yet to be reported
(13). In addition to RhoA, there are two other Rho isoforms in
mammals, RhoB, and RhoC, which are highly homologous, and
all three members induce stress fiber formation when overex-
pressed in fibroblasts (13). However, in knockout studies, RhoB-
null and RhoC-null mice are viable and have normal de-
velopment (17, 18). Recently, conditional deletion of RhoA in
the developing skin was reported, but the mutant mice did not
show any obvious phenotypes in vivo (19). Therefore, RhoA and
its close isoforms, RhoB and RhoC, may have redundant func-
tions in skin development. In mammalian epithelial cells, RhoA
induces actomysin contractility to expand cell–cell adhesion (20).
Furthermore, loss-of-function experiments using Drosophila em-
bryos demonstrated that the Drosophila RhoA homolog, Rho1,
is required for the organization of cadherin-based adherens
junctions (21, 22). These studies indicate that mammalian RhoA
may play a role in adherens junctions, but the physiological role
of RhoA in adherens junctions in mammalian nervous system
remains unknown.
In this study, we examined the role of RhoA in the developing
mouse brain using a conditional gene-targeting strategy with two
lines of Cre drivers (Wnt1-Cre for the midbrain and Foxg1-Cre
for the forebrain mutation). These conditional gene deletions
lead to similar phenotypes including the disruption of adherens
junctions, massive expansion of neural progenitors, and disor-
ganization of the brain. These findings uncover an essential and
nonredundant role of RhoA in neural progenitor cells in the
mouse central nervous system.
Localization of RhoA Protein in the Developing Brain. We first ex-
amined the spatial distribution of RhoA protein in the developing
brain. RhoA was detected throughout the neuroepithelium and
Author contributions: K.-i.K., R.A.L., C.-Y.K., Y.Z., and Y.Y. designed research; K.-i.K., J.M.,
J.M.B., J.R.L., B.K.C., and N.N. performed research; K.-i.K., J.M., C.-Y.K., Y.Z., and Y.Y.
analyzed data; and K.-i.K., C.-Y.K., Y.Z., and Y.Y. wrote the paper.
The authors declare no conflict of interest.
*This Direct Submission article had a prearranged editor.
1To whom correspondence should be addressed. E-mail: firstname.lastname@example.com.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.
| May 3, 2011
| vol. 108
| no. 18
| 7607–7612
Page 2
day 12.5 (E12.5) (Fig. 1A). Because the apical surface of the
ventricular zone is also enriched with cadherin–catenin proteins,
we compared the localization of RhoA with those of the adherens
that RhoA is extensively colocalized with αE-catenin, β-catenin,
iments further revealed the association of RhoA with β-catenin
interaction was supported by the reciprocal use of anti-RhoA
and anti–β-catenin antibodies in precipitation immunoblot but
yielded the same result, as well as the loss of the interaction signal
in conditional RhoA-null embryos (Fig. 1G; see below for condi-
tional deletion of RhoA). Together, these data suggest that RhoA
Deletion of RhoA in Mesencephalon Causes Exencephaly. To de-
termine the in vivo functions of RhoA in neural progenitors, we
generated RhoA-floxed mice (Fig. S1) and crossed them with
Wnt1-Cre mice that express Cre recombinase in the mesen-
cephalon as well as in neural crest derivatives (23). By crossing
with stop-floxed EGFP reporter mice, we detected the Wnt1-
Cre–mediated Cre/loxP recombination in the mesencephalon at
E9.5 (Fig. S2). In Wnt1-Cre; RhoAflox/flox(hereafter referred to as
RhoA-CKO) embryos, we validated the absence of RhoA ex-
pression in the mesencephalon by immunostaining, as well as
immunoblotting analysis (Fig. S3). Deletion of RhoA did not
markedly affect the expression of other small Rho GTPase
members (Fig. S3). At E13.5, RhoA-CKO embryos showed en-
largement of the mesencephalon, compared with their litter-
mates (Fig. 2 A and B, arrowheads), and at E15.5, they exhibited
exencephaly-like protrusions (Fig. 2 C and D). About 80% of
RhoA-CKO embryos exhibited exencephaly-like abnormalities
from E15.5 to E16.5 (Table S1). Histologically, the protrusion
consisted of a neuronal mass originating from the midbrain,
which not only exposed dorsally but also pushed forebrain
structures to the ventral side (Fig. S4). Because RhoA-CKO
embryos did not exhibit exencephaly-like abnormalities before
E12.5 (Table S1), we concluded that this anomaly was not caused
by the defects of neural tube closure. Rather, it is more likely to
be caused by abnormal expansion of neuronal cells.
RhoA Deficiency Disrupts Adherens Junctions at the Ventricular
Surface. We next examined Nissl-stained sections from RhoA-
CKO embryos and control littermates. The initial abnormality in
the RhoA-deleted mesencephalon was detected as multiple nod-
ules protruding from the apical surface around E11.5. This
anomaly intensified by E12.5, and the cellular organization in the
ventricular zone of the mesencephalon became highly irregular in
RhoA-CKO embryos (Fig. 3 A and B). After E12.5, the dysplasia
was observed in all of the RhoA-CKO embryos we examined. The
apical F-actin staining was lost in dysplastic regions whereas the
cellular organization at the basal side, including accumulation of
raising the possibility that RhoA deficiency may lead to disruption
of the cadherin-based apical cell–cell adhesion. Consistent with
this notion, we found that the localization of components of the
cadherin–catenin complex, including αE-catenin, β-catenin, and
N-cadherin, was all disrupted in the dysplastic region (Fig. 3 E–J).
These results suggest that RhoA deletion leads to disruption of
adherens junctions in the ventricular surface of the developing
Loss of RhoA Causes Massive Dysplasia in the Mesencephalon. At
E13.5, the dysplasia expanded throughout the mesencephalon
and the cellular organization was markedly disorganized in
RhoA-CKO embryos (Fig. S5 A and B). Nestin-positive neural
progenitor/radial glial processes were also disorganized in
RhoA-CKO embryos (Fig. S5 E and F). In addition, we fre-
quently found neuroblast rosette-like structures that were also
observed in αE-catenin and N-cadherin null mutants (6, 24) (Fig.
S5 C and D). In the midbrain of control embryos, Ki67+pro-
liferative cells and βIII-tubulin+postmitotic neurons were sep-
arated in the apical and basal compartments, respectively (Fig.
4A). However, these two cell populations were intermingled and
the ratio of postmitotic neurons decreased in RhoA-CKO em-
bryos [Fig. 4 B and C; control, 23.8 ± 3.4% (n = 3); RhoA-CKO,
16.5 ± 1.5% (n = 4); mean ± SD; P < 0.05 by Student's t test].
These results suggest that RhoA deficiency leads to massive
Fig. 1.
interacts with β-catenin. (A) Immunohistochemistry for RhoA revealed that
RhoA protein is enriched at the apical portion of the ventricular zone of the
mesencephalon at E12.5. RhoA-positive signals colocalize with positive sig-
nals for αE-catenin (B), β-catenin (C), and N-cadherin (D–F) at E13.5. Nuclei
are visualized by ToPro3. (Scale bars: A, 50 μm; B, 10 μm.) (G) Immunopre-
cipitation revealed the interaction of RhoA with β-catenin, and the in-
teraction was not observed in conditional RhoA-null (RhoA-CKO) brain.
RhoA accumulates at adherens junctions in the ventricular zone and
Fig. 2.
subsequently leads to exencephaly-like protrusion formation. General ap-
pearance of control (A and C) and RhoA-CKO (B and D) embryos at E13.5 (A
and B) and E15.5 (C and D).
Deletion of RhoA induces enlargement of the mesencephalon and
| www.pnas.org/cgi/doi/10.1073/pnas.1101347108 Katayama et al.
Page 3
dysplasia and expansion of neural progenitor cells in the de-
veloping mesencephalon.
Accelerated Proliferation and Decreased Cell-Cycle Exit in RhoA-
Deleted Neural Progenitor Cells. To assess whether the loss of
RhoA leads to changes in cell proliferation, we performed
immunostaining with anti–phospho-histone H3 (p-H3) antibody
to identify mitotic cells. In control embryos, p-H3–positive nuclei
were localized close to the ventricular surface of the neuro-
epithelium (Fig. 4D). In contrast, in RhoA-CKO embryos, the p-
H3–positive nuclei were dispersed throughout the brain (Fig.
4E). Quantitative analysis of the p-H3 staining demonstrated
about a twofold increase in the number of mitotic cells among
total neuronal cells in the mesencephalon in RhoA-CKO em-
bryos [Fig. 4F; control, 2.8 ± 0.3% (n = 4); RhoA-CKO, 5.1 ±
0.8% (n = 4); P < 0.01].
Enlargement of the progenitor pool in RhoA-deficient mid-
brain may result from accelerated proliferation, decreased cell
death, failure in cell-cycle exit, or any combination of these
factors. First, to reveal potential changes in the proliferation, we
counted the proportion of neural progenitor cells labeled by
a 30-min pulse of BrdU and found a significant increase in the
fraction of BrdU-labeled nuclei among all Ki67+neural pro-
genitor cells in RhoA-CKO embryos [Fig. 4 G–I; control, 52.9 ±
3.1% (n = 5); RhoA-CKO, 66.5 ± 4.6% (n = 3); P < 0.01]. The
increase of BrdU+fractions among Ki67+cells suggests accel-
erated proliferation of neural progenitor cells. In addition,
BrdU-positive nuclei of control embryos localized at the basal
side of the ventricular zone (Fig. 4G), whereas those of RhoA-
CKO embryos localized not only at the basal side but also at the
apical side (Fig. 4H).
To examine whether loss of RhoA causes changes in cell-cycle
withdrawal, we counted the proportion of cells that exit the cell
cycle after 24 h labeling with BrdU, which was calculated as the
ratio of BrdU+/Ki67−cells among all BrdU-labeled cells. This
analysis revealed a significant decrease of the cell-cycle exit index
in RhoA-CKO embryos, compared with control embryos [Fig. 4
J–L; control, 34.0 ± 2.1% (n = 6); RhoA-CKO, 22.9 ± 3.3%
(n = 3); P < 0.01].
Finally, we compared the extent of apoptosis using the terminal
deoxynucleotidyl transferase-mediated dUTP nick-end labeling
(TUNEL) method and found that the number of apoptotic cells
was not decreased but rather increased in RhoA-CKO embryos
(Fig. S6). Thus, our results suggest that expansion of neural pro-
genitor cells in the RhoA-deleted midbrain is caused by both ac-
celerated proliferation and failure to exit the cell cycle.
Similar Defects in Adherens Junctions and Expansion of the Neural
Progenitor Pool in Forebrain-Specific RhoA Deletion. To examine
whether RhoA has important neurodevelopmental functions
outside the mesencephalon, we crossed RhoA-floxed mice with
another Cre-driver line, Foxg1-Cre. In Foxg1-Cre mice, the Cre
recombinase was expressed by telencephalic progenitors from
E9.5 (25). Genotyping by PCR detected near-complete re-
combination of the floxed RhoA allele in the forebrain, but not
the tail, of Foxg1-Cretg; RhoAflox/flox(hereafter referred to as
Foxg1-RhoA-CKO) embryos at E13.5 (Fig. 5B). From E13.5 to
E14.5, Foxg1-RhoA-CKO embryos exhibited an increasing in-
cidence of the exencephaly phenotype (55% at E13.5 and 80% at
E14.5, Fig. 5A and Table S1), similar to the Wnt1-Cre–mediated
RhoA-CKO embryos. Histologically, the mass consisted of
Pax6+neural progenitors, which were greatly expanded com-
pared with control embryos (Fig. 5 C and D). The ventricular
surface was disorganized and the apical expression of αE-catenin
became absent in Foxg1-RhoA-CKO embryos, Instead, there
were rings of intense αE-catenin expression inside the brain mass
in mutant embryos (Fig. 5 E and F). Ki67+and Pax6+progen-
itors either were extensively intermingled with βIII-tubulin+
postmitotic neurons (Fig. 5 G and H) or formed rosette-like
clusters in E14.5 Foxg1-RhoA-CKO embryos, similar to those
detected in Wnt1-Cre–derived embryos (Fig. S5 G and H). To-
gether, the similar phenotypes in midbrain and forebrain suggest
that RhoA may have important functions in neuroepithelial
progenitors throughout the developing nervous system.
Downstream Targets of the Hedgehog Signaling Pathway. Finally,
we turned to the issues of signaling pathways responsible for the
abnormality of neural progenitors in RhoA-CKO embryos. In this
context, previous studies of mutants with a disrupted cadherin–
catenin complex reported that αE-catenin null mice exhibit abnor-
mal activation of the hedgehog pathway (6) and that deletion of
Lgl1 causes loss of cell polarity and up-regulation of the Notch
known to induce hyperproliferation in neural progenitor cells (26).
To compare the consequences of RhoA deletion with the
previous studies, we examined the expression of transcriptional
targets of hedgehog, Notch, and Wnt/β-catenin pathways by re-
verse transcription–quantitative PCR analysis. We found that the
expression of Gli1 and Fgf15 was significantly elevated in the
RhoA-deficient midbrain at E13.5 (Fig. 6A). The in situ hybrid-
ization analysis showed that Gli1 and Fgf15 transcripts were
MesencephalonShowsIncreased Expressionof
Fig. 3.
cells. (A and B) Nissl-stained sections through the developing mesencephalon
from control (A) and RhoA-CKO (B) embryos at E12.5. The apical structure is
partially disrupted in RhoA-CKO embryos, and neural progenitor cells are
irregularly arranged and protruded to the ventricle in this region (B). (C and
D) Double immunostaining for F-actin and βIII-tubulin of control (C) and
RhoA-CKO (D) embryos. Apical F-actin staining is disrupted in the dysplastic
region, whereas basal βIII-tubulin (TuJ1) staining remains unaffected (D). (E–
I) Immunostaining for αE-catenin (E and F), β-catenin (G and H), and N-
cadherin (I and J) of control (E, G, and I) and RhoA-CKO (F, H, and J) embryos
at E12.5. Apical staining of these molecules is also disrupted in the dysplastic
region. (Scale bars, 50 μm.)
Disruption of adherens junctions in RhoA-deleted neural progenitor
Katayama et al.PNAS
| May 3, 2011
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Page 4
markedly up-regulated in neural progenitor cells of the RhoA-
deficient midbrain at E13.5 (Fig. 6 B–E). We also found a mild
but significant increase in the expression of Notch pathway target
genes (Hes5 and Hes1) and a Wnt/β-catenin target gene
(CyclinD1) (Fig. 6A). However, because these three genes are
highly expressed in neural progenitor cells (27, 28) (Fig. 6 F and
G), the slight increase in the expression of these genes may re-
flect the expansion of neural progenitor cells in RhoA-CKO
embryos (Fig. 4 A–C). Indeed, the expression of Sox2, a marker
for neural progenitor cells, was increased in RhoA-CKO em-
bryos similar to that of Hes5, Hes1, and CyclinD1 (29) (Fig. 6A).
In addition, because there were no significant changes in the
expression of Axin2 and c-myc (Fig. 6 A, H, and I), the Wnt/
β-catenin pathway is unlikely to be involved in the hyper-
proliferation of neural progenitor cells in RhoA-CKO embryos.
Together, these results suggest that the hedgehog pathway is
activated in RhoA-deficient neural progenitor cells.
RhoA, a founding member of the small Rho GTPase family, has
been implicated in a host of cellular processes, but its physio-
logical functions remain uncertain due to the lack of specific loss-
of-function studies (13). In the present study using two lines of
Cre drivers to delete RhoA in the midbrain or forebrain early in
development, we showed that RhoA deficiency resulted in dis-
ruption of adherens junctions, hyperproliferation of neural pro-
genitors, and brain dysplasia. These results demonstrated that
RhoA has important and indispensable functions in mammalian
brain development.
RhoA Is Indispensable for the Adherens Junction Formation in the
Developing Brain. The neuroepithelium of developing mammalian
brains has a string of adherens junctions that connects neural
progenitors at the ventricular surface, similar to apical epithelial
junctions (4). The main building blocks of adherens junctions are
cadherins and catenins, and in the developing mammalian
brains, the critical components are N-cadherin, β-catenin, and
αE-catenin, according to their unique gene-expression patterns
and null-mutation phenotypes (5). In addition, cell-culture stud-
ies suggest that small Rho GTPases—especially Rac1, Cdc42, and
RhoA—are important components of adherens junctions, regu-
lating the interactions between catenins and the cytoskeleton
Fig. 4.
C) Immunohistochemistry for Ki67 and βIII-tubulin revealed increased Ki67+neural progenitor cells and decreased βIII-tubulin+postmitotic neurons in RhoA-
CKO embryos. The graph represents ratios of βIII-tubulin+postmitotic neurons to the total neuronal cell number. (D–F) Immunostaining for p-H3 revealed
increased mitosis in the RhoA-deficient mesencephalon. The graph shows ratios of mitotic cells to the total neuronal cell number. (G–I) Accelerated pro-
liferation in RhoA-deficient neural progenitors. A higher percentage of RhoA-deficient progenitors (Ki67+) are labeled with BrdU after a 30-min pulse. The
percentages of K67+cells that incorporated BrdU are shown. (J–L) Cell-cycle withdrawal is compromised in RhoA-deficient neural progenitors. Pregnant mice
were injected with BrdU 24 h before being killed. Cells reentering the cell cycle are BrdU+/Ki67+, whereas cells withdrawn from the cell cycle are BrdU+/Ki67−.
Cell-cycle exit was determined as the ratio of cells that exited the cell cycle (BrdU+/Ki67−) to all cells that incorporated BrdU. All these analyses were carried out
using E13.5 embryos. The graphs represent mean + SD. n = 3–6, *P < 0.05, **P < 0.01, Student's t test. (Scale bars, 50 μm.)
Accelerated proliferation and failure in cell-cycle withdrawal result in expansion of neural progenitor cells in the RhoA-deficient mesencephalon. (A–
| www.pnas.org/cgi/doi/10.1073/pnas.1101347108 Katayama et al.
Page 5
(11, 12). In our study, we showed that RhoA is concentrated at
the apical surface of developing brains and colocalized with
cadherin–catenin proteins. RhoA-deleted embryonic brain showed
disruption of adherens junctions and severe dysplasia, similar to
the phenotypes of αE-catenin, β-catenin, and N-cadherin mutant
mice (6, 24, 30). These results together with the finding that
RhoA physically interacts with β-catenin strongly suggest that
RhoA is an integral and indispensable component of adherens
junctions in the developing mammalian brain.
Differential Roles of RhoA and Cdc42 in Adherens Junctions of Neural
Progenitors. Although Cdc42 deletions in previous reports also
showed their essential roles in adherens junctions in the nervous
system (14, 15), there are also significant differences in the
phenotypes of RhoA and Cdc42 deletions, even when the same
Cre driver (i.e., Foxg1-Cre mice) was used. In Foxg1-Cdc42 null
embryos, the disappearance of apical cadherin–catenin proteins
started as early as E9.5–E10.5, causing holoprosencephaly due to
failure to expand the telencephalon (15). In contrast, RhoA de-
letion by Foxg1-Cre or Wnt1-Cre drivers caused a gradual dis-
ruption of apical cadherin–catenin protein localization between
E11.5 and E14.5. Although we cannot exclude the possibility that
the distinct phenotypes may be in part due to different amounts
of residual RhoA and Cdc42 protein after Cre/loxP recombi-
nation, it is likely that Cdc42 has essential functions for the initial
establishment of apical adherens junctions, whereas RhoA is
essential for their maintenance in the developing mammalian
brain. This interpretation is in keeping with the notion that dif-
ferent small Rho GTPases are involved in different steps of the
adherens junction formation, expansion, and plasticity (11, 12).
Dysregulated Proliferation of RhoA-Deficient Neural Progenitor Cells.
During brain development, proliferation, differentiation, and cell
death of neural progenitor cells are tightly regulated to produce
the organ of predetermined size (1–3). Recent studies using
mutant mice have shown the coincidence of defects in adherens
junctions and hyperproliferation of neural progenitor cells, in-
dicating the involvement of adherens junctions in this process
(5). For example, deletion of αE-catenin in the developing ce-
rebral cortex leads to defects in adherens junctions, massive
disorganization of the neuroepithelium, and hyperproliferation
of neural progenitor cells (6). Although the molecular mecha-
Fig. 5.
tions and expansion of neural progenitor cells, leading to exencephaly-like
protrusion formation. (A) Gross appearance of control (Left) and Foxg1-
RhoA-CKO (Right) embryos at E14.5. (B) PCR analysis showed near-complete
recombination of the floxed RhoA allele in the forebrain, but not the tail, of
Foxg1-RhoA-CKO embryos at E13.5. (C and D) At E13.5, Foxg1-RhoA-CKO
embryos exhibited expansion of the Pax6+cell population, which formed
masses in the developing cerebral cortex. (E and F) At E14.5, the apical/
ventricular wall became disrupted and the localization of αE-catenin was lost
in Foxg1-RhoA-CKO embryos. (G and H) Whereas Ki67+neural progenitor
cells and βIII-tubulin+postmitotic neurons were completely segregated in
control embryos (G), these two components were extensively intermingled
and neural progenitor cells were expanded in Foxg1-RhoA-CKO embryos (H).
(Scale bars: C and D, 100 μm; E–H, 50 μm.)
Forebrain RhoA deletion also causes disruption of adherens junc-
Fig. 6.
hog signaling pathway in the RhoA-deficient mesencephalon. (A) Reverse
transcription–quantitative PCR analysis of Gli1, Fgf15, Hes5, Hes1, Axin2,
c-myc, CyclinD1, and Sox2 in control and RhoA-deficient midbrains at E13.5.
The graphs represent mean + SD. n = 5 for control and n = 7 for RhoA-CKO
embryos, *P < 0.05, **P < 0.01, Student's t test. (B–I) In situ hybridization for
Gli1 (B and C), Fgf15 (D and E), Hes5 (F and G), and Axin2 (H and I) of the
mesencephalon from control (B, D, F, and H) and RhoA-CKO (C, E, G, and I)
embryos at E13.5. (Scale bar, 50 μm.)
Increased expression of downstream target genes of the hedge-
Katayama et al.PNAS
| May 3, 2011
| vol. 108
| no. 18
| 7611
Page 6
nisms that link adherens junctions and proliferation control re-
main unclear, recent evidence suggests the involvement of major
developmental signaling pathways as a cause of hyperprolif-
eration (5). In αE-catenin mutant mice, the expression of Gli1
and Fgf15, targets of the hedgehog pathway, was up-regulated,
and the activation of the hedgehog signaling pathway is likely
to explain the hyperproliferation of neural progenitors (6). We
found that the expression of Gli1 and Fgf15 was markedly in-
creased in RhoA-CKO embryos, similar to that in the αE-catenin
null mutants. Importantly, the hedgehog pathway plays a critical
role in brain development and cancer, and Gli1 promotes pro-
liferation of neural progenitor cells in the developing brain (31,
32). Thus, the hyperproliferation of neural progenitor cells in
RhoA-CKO embryos may be caused, at least in part, by hyper-
activation of the hedgehog pathway.
In conclusion, the present study identifies RhoA as a critical
protein for the maintenance of adherens junctions and con-
strained proliferation of neural progenitors in the developing
mammalian brain (Fig. S7).
Materials and Methods
Animals. Mice harboring conditional RhoA alleles, in which exon 3 is flanked
by loxP sites, were generated as described in Fig. S1.
Histological Analysis. Nissl staining, in situ hybridization, and immunohisto-
4% paraformaldehyde, cryoprotected in 30% sucrose, embedded in OCT
compound, and sectioned at 16 μm. Antibodies used in this study are listed in
Table S2. To detect apoptotic cells, we carried out the TUNEL method using
the ApopTag fluorescein in situ apoptosis detection kit (Millipore). We also
used phalloidin-tetramethylrhodamine B isothiocyanate (Sigma) and ToPro3
or DAPI (Molecular Probes) to visualize F-actin and nuclei, respectively.
Images were taken by an Axio Imager Z1 microscope and an LSM510 con-
focal microscope (Carl Zeiss).
Quantitation of Cell Differentiation, Mitosis, Proliferation, Cell-Cycle Exit, and
Cell Death. BrdU labeling and analysis of cell-cycle exit were performed as
described (6, 8, 26). For quantitation of the data, immunohistochemical
images were taken by an Axio Imager Z1 microscope (Carl Zeiss) and the
total numbers of cells and cells stained with indicated antibodies were
counted using AxioVision software (Carl Zeiss).
Immunoprecipitation. Midbrains from E13.5 embryos were lysed in tissue lysis
buffer [20 mM Tris-HCl (pH 7.6), 100 mM NaCl, 10 mM MgCl2, 1% Triton-
X-100, 0.2% sodium deoxycholate, 2 mM phenylmethylsulphonyl fluoride,
100 nM okadaic acid, and a mixture of protease inhibitors]. Supernatants
were exposed to the antibodies. Then, the immunocomplexes were captured
by adding Protein A/G-agarose beads (Santa Cruz Biotechnology).
RNA Extraction and Reverse Transcription–Quantitative PCR. Midbrains were
dissected out from E13.5 embryos and total RNA was extracted using the
RNeasy Micro Kit (Qiagen). Reverse transcription was performed with Su-
perScript III reverse transcriptase (Invitrogen). Real-time PCR was performed
with QuantiTect (Qiagen) by the CFX96 Real-Time System (Bio-Rad). The
intensity relative to β-actin was calculated, and the fold change relative to
the relative intensity in control embryos is presented. Primers were designed
by Primer3 (http://frodo.wi.mit.edu/primer3/).
ACKNOWLEDGMENTS. The authors are grateful to Dr. Masato Nakafuku
and Dr. Akira Nagafuchi for providing us with antibodies. The authors thank
Dr. Fumiyasu Imai for critical comments on the manuscript. K.-i.K. is
supported by Japan Society for the Promotion of Science Postdoctoral
Fellowships for Research Abroad.
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contraction and directed migration of keratinocytes. Mol Biol Cell 22:593–605.
20. Yamada S, Nelson WJ (2007) Localized zones of Rho and Rac activities drive initiation
and expansion of epithelial cell-cell adhesion. J Cell Biol 178:517–527.
21. Bloor JW, Kiehart DP (2002) Drosophila RhoA regulates the cytoskeleton and cell-cell
adhesion in the developing epidermis. Development 129:3173–3183.
22. Magie CR, Pinto-Santini D, Parkhurst SM (2002) Rho1 interacts with p120ctn and
alpha-catenin, and regulates cadherin-based adherens junction components in
Drosophila. Development 129:3771–3782.
23. Hsu W, Mirando AJ, Yu HM (2010) Manipulating gene activity in Wnt1-expressing
precursors of neural epithelial and neural crest cells. Dev Dyn 239:338–345.
24. Kadowaki M, et al. (2007) N-cadherin mediates cortical organization in the mouse
brain. Dev Biol 304:22–33.
25. Hébert JM, McConnell SK (2000) Targeting of cre to the Foxg1 (BF-1) locus mediates
loxP recombination in the telencephalon and other developing head structures. Dev
Biol 222:296–306.
26. Chenn A, Walsh CA (2002) Regulation of cerebral cortical size by control of cell cycle
exit in neural precursors. Science 297:365–369.
27. Ohtsuka T, et al. (2006) Visualization of embryonic neural stem cells using Hes
promoters in transgenic mice. Mol Cell Neurosci 31:109–122.
28. Glickstein SB, Alexander S, Ross ME (2007) Differences in cyclin D2 and D1 protein
expression distinguish forebrain progenitor subsets. Cereb Cortex 17:632–642.
29. Ellis P, et al. (2004) SOX2, a persistent marker for multipotential neural stem cells
derived from embryonic stem cells, the embryo or the adult. Dev Neurosci 26:
30. Machon O, van den Bout CJ, Backman M, Kemler R, Krauss S (2003) Role of beta-
catenin in the developing cortical and hippocampal neuroepithelium. Neuroscience
31. Ruiz i Altaba A, Palma V, Dahmane N (2002) Hedgehog-Gli signalling and the growth
of the brain. Nat Rev Neurosci 3:24–33.
32. Palma V, Ruiz i Altaba A (2004) Hedgehog-GLI signaling regulates the behavior of
cells with stem cell properties in the developing neocortex. Development 131:
| www.pnas.org/cgi/doi/10.1073/pnas.1101347108 Katayama et al.


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"Painful to listen to"
I've never read anything by Wayne Simmons before, however, after struggling to get 1/4 through this book, and finally giving up, I don't think I'll ever read anything by him again. I finally just gave up and called it unreadable.
I've yet to figure out who the main char. is, or what the story is about - it starts off as a typical Zombie story, people turning undead, then wanders off into some girl that's being held captive by some guy. From there it only got worse.
Report Inappropriate Content
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text:
i dont normally do this but holy hell....wow....this is $240 dollars worth of beer. to most people that sounds stupid as hell but its worth it to me.
thats 04 and 05 epic, 3rd, 7th and 8th anniversary, 2000 old guardian, and 2003 double bastard.
hahaha. i feel like a kid again! *and yes, thats sad*.
You finally made it home! Draped in the flag that you fell for. And so it goes, the ashes of the wake!
Great score, where'd you find '04 and '05 Vertical Epic?
this place called "stuffed sandwitch" that has kick ass food and very rare beers. its stone month there, so they had their old stone beers out for sale.
You spent $240 on seven beer??????????????????
Is this a sign that society has reached the end? Does everyone have that much 'disposable' income that it doesn't matter?
More power to you if you can do it...but wow...
Not to change the subject, but could you share the recipe for the Tripel Karmeliet clone? I'm interested in brewing something of that variety.
I was just talking to Greg Koch from Stone yesterday... he would have been proud.
[Edit: I should clarify -- I don't normally get a chance to hobnob with microbrewery CEOs... He was in town at a local pub for a small beer-fest and I had the opportunity to hang out for a while.]
So, what are we drinking first?
oh god i dont know! i really really want to crack that 2000 guardian. i think the 10 year old ipa would not taste so good at this point haha.


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Can anyone tell me to tell the difference in male and female cantaloupe flowers. This is my first garden and I have some contaloupe plants growing and thay are starting to get these small yellow flowers and from what I read they all look like male flowers. Can anyone out there post a photo so i can toll the difference.
I think a female flower will have a cantelope underneath it as the same with squash. The male has no squash- the female does. Where are you that you already have flowers on your cantelope. I haven't even planted anything yet. Its going to be 37 degrees tonight. I'm jealous- your doing okay by me if you've got flowers. Be patient, once the flower has beeen pollinated- you'll get a cantelope. Happy gardening
The link below has a picture of a female cantaloupe blossom which may or may not be pollinated. If not pollinated, the small fruit/blossom will fall off the plant. OTOH, male blossoms are attached by a straight stem to the plant and don't have that small fruit bulge.
Most cucurbits seem to open a bunch of male blossoms before the female blossoms appear, so you may not have any females yet.
Patience is indeed the key. Even the first few female blossoms may not be pollinated until the busy insects find them and go to work. But it will happen sooner or later.
Here is a link that might be useful: Female cantaloupe blossom


text:
We found 1 site within 15 miles of Shrewsbury with 1 bookable
Ellesmere, Shropshire, England Show on map
|Otter pod
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LibreOffice 4.2 Brings OpenCL Calc, OOXML Improvements
Published on 30 January 2014 10:39 AM EST
Written by
Michael Larabel
5 Comments
A new stable, major release of the open-source LibreOffice suite is now available and with it comes several new and improved features.
LibreOffice 4.2 provides improved Microsoft OOXML interoperability, a new engine for the Calc spreadsheet, WIndows-specific improvements including better Windows 7/8 integration, a new expert configuration window, a new start screen, new icons, various bug-fixes, and other improvements. With the new LibreOffice Calc engine comes the OpenCL GPGPU support.
More details on the LibreOffice 4.2 improvements can be found via
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Kansas at your Service is a free, online customer service training. For more information or to take the course, click on the logo above.
If photography is your inspiration, Great Bend is your natural wonderland!
Photographers delight in capturing wildlife in their natural habitats. Magazines often feature impressive wildlife and landscape shots from our region of Kansas. Below is a list of Great Bend hot spots, check out the brochure download for a full list.
To view the Photography Brochure for maps, hot spots, more tips, and additional information, click 'view' under the link. You can download the PDF document by clicking 'download', or email it by selecting email below.
3007 10th Street Great Bend, KS 67530 | (620) 792-2750 or (877) 427-9299 | email@example.com


text:
marysnest's ideas
I love a cozy nook and plaid makes it even better.
Love the bookshelves.
Love the plaid chairs and the charming yellow hutch
Cute idea using a tartan blanket as a table cloth.
Built-in bookcases are fabulous.
Bookcases are wonderful and love the over all colonial feel to the room.
Red Tartan Pillow - $49
Too cute!
Red Plaid Ice Bucket - $39.99
Very nice.
Royal Stewart Tartan Desk Set - $119
Adorable uses for tartan plaid!
Light, bright, yet very cozy kitchen. Love the fireplace.
Plate racks are great. Makes putting dishes "away" so easy!
Once again, love using a tartan plaid blanket for a table cloth.
Love this reading nook!
Lovely entry - love the lamp with the black shade.
Love this little reading nook. Cozy with a touch of femininity.
Love the bookshelves. Room almost looks oval. Is it?
I have this chair! I grew up with it in my parents home and now it sits at my desk.


text:
Grunt plugin for testing requireJS code using mocha in phantomJS and regular browsers
Grunt plugin for testing requireJS code using mocha in phantomJS and regular browsers
The purpose of this grunt plugin is as follows:
- To provide a simple structure for writing unit tests for requireJS applications without duplicating the runner HTML.
- To allow for automated running of unit tests using phantomJS.
- To provide easy switch to manually run unit tests in a regular browser (because sometimes you just need to debug using a browser console).
Getting Started
This plugin requires Grunt
If you haven't used Grunt before, be sure to check out the Getting Started guide, as it explains how to create a Gruntfile as well as install and use Grunt plugins. Once you're familiar with that process, you may install this plugin with this command:
npm install grunt-mocha-require-phantom --save-dev
The "mocha_require_phantom" task
In your project's Gruntfile, add a section named
mocha_require_phantom to the data object passed into
gruntinitConfig mocha_require_phantom: options: base: 'some/path/to/script' main: 'test-bootstrap' requireLib: 'libs/require.js' files: 'tests/**/*.js' your_target: // Target-specific file lists and/or options go here. ;
Default value:
' '
Path to where your script files are within your project. Assumes that all source, libraries and test script files are within this directory.
Default value:
The path to your test bootstrap file that will be specified in requireJS's data-main attribute.
Default value:
The attribute appended to the script tag that specifies the module defined in options.main.
Default value:
The path to the requireJS library within your application.
Default value:
An array of paths to your test files.
Default value:
The port that the server will be run on.
Default value:
Setting this option to true will keep the server alive and the task will never complete. The purpose of this is to allow you to debug your unit tests within a browser environment if necessary (sometimes you just need a browser's dev tools).
Command line Options
Comma-separated list of files to test relative to the "base" option. If this option is passed in then the list of files will take precedence over the files passed in via the GruntFile.js.
Test bootstrap file
This file is used to kick off your test code. You should do any requireJS and Mocha configuration in this file. e.g.
requireconfig paths: chai: '/node_modules/chai/chai' ; mochasetup ui: 'bdd';
Test file path name
Within your bootstrap file you will need to use requireJS to load your unit tests and execute Mocha. To do this a global var named testPathname is made available and should be used like so:
require //require other modules from your application if necessary here, testPathname ifwindowmochaPhantomJS mochaPhantomJSrun; else mocharun; ;
Test files
You do not need to create any HTML runner files. Simply create an AMD module that runs your test like so:
define 'chai' //define application modules to be tested here var expect = chaiexpect; describe'test suite 1' it'should work' expecttruetoequaltrue; ; ; ;
Browser testing
The main purpose of this plugin is to provide automated testing via phantomJS. However, you can manually run tests in regular browsers also - sometimes this is necessary for debugging. To do so you need to
- set the keepAlive option to true.
- load up your favourite browser and enter the following url: http://localhost:3000/path/to/test-file
In lieu of a formal styleguide, take care to maintain the existing coding style. Add unit tests for any new or changed functionality. Lint and test your code using Grunt.
Release History
- 2014-06-05 v0.4.0 Main attribute can be specified as an option.
- 2014-06-05 v0.3.1 Only serve test runner page if request begins with base option.
- 2014-05-23 v0.3.0 Specific test files can now be passed in via command line options.
- 2014-05-12 v0.2.2 Bumped version after merged pull request that fixes issues with passing console.log statements through to command line.
- 2014-05-12 v0.2.0 console.log statements now make it through from phantomJS to command line.
- 2014-05-06 v0.2.1 Bumped version after merged pull request that uses Chai's built in expect messages. Changed console.log output to be comma-separated on one line.
- 2014-05-05 v0.1.5 Amended docs.
- 2014-05-05 v0.1.4 Fixed broken example by adding require's baseUrl config option.
- 2014-02-10 v0.1.3 Normalised server requests between automated and manual tests.
- 2013-12-17 v0.1.2 Don't run tests in PhantomJS if keepAlive set to true to stop task failing.
- 2013-12-17 v0.1.1 Copy mocha JS and CSS files to temp directory to fix issues.
- 2013-12-17 v0.1.0 Initial release.


text:
User Tip: If you're unsure of your vehicle type, you may skip the machine type selection box.
Please select make, model and yearAir Filters
Air filters made from the best materials by K & N, UNI, No Toil, Twin Air and many others. Find the best in stock selection at the lowest prices around.
Steve in NC
This product works great as it says. Used these pre filter on my sons bikes at a LQ last in NC. The track was a mess due to rain and the track was sand and clay mix. I cleaned the bikes to get ready for the next weekend for the pro shoot out in VA. Took the seat off and with a little tug on the string pre filter came right off and the filter was clean I was shocked. They are a pain to on but great product.Read All Reviews
MARK in VA
Far better than stock, holds up well and easy to clean and re-oil.Read All Reviews
Claudio in FL
Good air filter system.. better performanceRead All Reviews
marc in NJ
this should be called "no muss, no fuss"... these things are great... especially for a spare... i don't know about you all, but sometimes i rather go to walmart and get a new bed in a bag then to do laundry and clean my existing ones.... lol i'm still a man, a savage, an animallllllll!!!!! so these are an extension of that thought process.... clean, fresh, new.... what more could you want for a few bucks....Read All Reviews
Tanner in KY
Easy cheap, worth the money if your in a pinch, especially with RM quick shipping !Read All Reviews
Ali in WA
These are great have saved costs on buying filters . Great buyRead All Reviews


text:
My daughter and son-in-law just had a baby last week. We are thrilled. But we won't be able to see the baby or hold her any time soon. We won't be able to take over little gifts or help out with housework or babysitting.
Discuss the article on the Forums.
Extragastrointestinal manifestations of Helicobacter pylori infection: Facts or myth?
Discussion in 'Other Health News and Research' started by nanonug, Apr 21, 2012.
Page 1 of 2
Page 1 of 2


text:
"Entry Level "
- Comp & Benefits
- Work/Life Balance
- Senior Management
- Career Opportunities
I worked at Xentel
Easy money and cash bonuses.
Working environment needs working on.
Better management.
- Any Location
- Any Job Title
"Good part-time job for students "
- Comp & Benefits
- Work/Life Balance
- Senior Management
- Career Opportunities
I have been working at XentelPros
Nice bonuses, fun environment, flexible schedules and close to Skytrain. They also have foosball and TV. Most of the supervisors are friendly and nice to work with!Cons
Can be stressful if you are not getting enough donations. Also, it is hard to get the bonuses because you need to ask for credit cards.Advice to ManagementAdvice
Provide better training and discourage employees from smoking outside the office. Also, provide free food events and parties to celebrate goals.RecommendsApproves of CEO
1 person found this helpful
"Telemarketing isn't for everyone. "
- Comp & Benefits
- Work/Life Balance
- Senior Management
- Career Opportunities
I worked at XentelPros
Personal & professional goals are attainable on a regular basis. Great way to sharpen the sales monster inside oneself. Management position was fairly stress free. Reasonably enjoyable job.Cons
Very poor compensation. I co-managed an office containing 50-70 agents and made $24,700/yr. Constant turnover. Have to deal with lower class and young employees on a regular basis. Team morale is generally low despite management's efforts to correct this.Advice to ManagementAdvice
Pay your agents and office management considerably more. This will minimize the trash that generally makes up your employ, increase professionalism and improve team morale. Update equipment to a competitive level.Doesn't RecommendDisapproves of CEO


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[March 04, 2014]
Introducing Innoprise in the Cloud
(ENP Newswire Via Acquire Media NewsEdge) ENP Newswire - 04 March 2014 Release date- 03032014 - Harris ERP is pleased to announce that GEMS and SELECT customers wanting to move to Innoprise will be able to select from two cloud-based ERP options, in addition to our traditional on-premise option: Cloud 9 Harris ERP offers Innoprise applications in a traditional Software as a Service (SaaS) model.
No hardware for you to worry about, as everything is hosted in our new data center (or you can select for your own data center); you pay no licensing fees; and we manage the servers and environment.
Local Cloud For those who are not comfortable having their data residing in the cloud, Harris ERP offers Innoprise applications as a managed service behind your firewall. We put the servers into your building and manage theapplications remotely. Again, there are no licensing fees, just a service subscription.
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Ben Philippi is an award-winning producer and photographer based in North America. He has created content for History Channel, A&E, National Geographic, Les 7 Doigts de la Main, Cirque du Soleil, Cirque Eloize, ARTV, Zone 3, Sid Lee and many more.
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No longer living in Ferrari's shadow, the DeTomaso Pantera has reached genuine celebrity status. Better yet, the Italian-American hybrid delivers neck-snapping performance thanks to... More
DeTOMASO: 1988 Pantera GT5S coupe, white with black interior. Hyman Ltd, 314-524-6000, MO; www.hymanltd.com... More
You need one of these in your collection! A 23K original mile, two owner, well maintained California delivered Pantera. Finished in bright Yellow with... More
This Italian sports car is undeniably rare, one of only 7,260 produced over 2 decades! It features a 351 ci Cleveland V8 engine and... More
1972 DeTomaso Pantera '72 DeTomaso Pantera, white with racing script, very cool car. Excellent foundation for restoration. For $19,750 USD If you have any additional questions Please... More
1972 DeTOMASO PANTERA 4 BOLT MAIN 310HP 351 CLEVELAND MOTOR ZF 5 SPEED TRANSMISSION RED WITH BLACK INTERIOR AN EXCEPTIONALLY ORIGINAL, 22,447 MILE CAR ALL ORIGINAL INTERIOR IN OUTSTANDING CONDITION -... More


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San Francisco, CAUSA 94131
October 31, 2012
Deleted excerpts from her fiction. All her fiction.
Every day, in the way I deal with & interact with other people on every level.
Long time Ayn Rand devotee
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Why Shop Midtown Comics• Big Savings with Advance Previews • Free Shipping on Qualified Orders • Testimonials
Writer: Grant Morrison • Artist: Rags Morales, Various
Superman is on trial for his life - and the jury is the Anti-Superman Army! It's the ultimate villains springing the ultimate trap at the end of time itself! And the mystery that has built since issue #1 is resolved as the Little Man's true identity is revealed - with grave consequences! Plus, in the backup feature: A crucial piece of information is revealed! This issue is also offered as a combo pack edition with a redemption code for a digital download of this issue.
Item #: 1231955 • Diamond code: OCT120171 • UPC: 76194130637701541


text:
Getting back into the swing of things on campus we teamed up with ENACT to bring all of the sustainably minded people at Muhlenberg together for one fun night of organic, vegan wraps and other yummy foods. As the garden is covered in some inches of snow at the moment, we decided to take the festivities indoors. Before we knew it, double the amount of people we were expecting were sitting in a massage circle laughing and talking. We even got a surprise visit from Professor Turk and her lovely family. She brought delicious quinoa and cookies! We then took a group trip to the compost cages with the waste we generated during cooking and looked at the fantastic full moon. Janki, a sustainably minded freshman, also brought smoothies. Later, we played cards against humanity as a group, jammed out on guitar, and ate vegan desert that we baked together.
Sustainable living is about bringing together communities and using less energy to feed people. The dinner's title, Date Your Mother (Earth) was about the idea of reconnecting to the Earth. The only phones that were out were ones used to take pictures of each other and our silly food faces, but we all took the time to connect to each other.
Peace & All of the Tree Hugging Love,
Rebecca & Neshama
Special shout out to Senior, Sarah for promising to read this blog.


text:
CMRL pointed to poor civic infrastructure around the MRTS between Chennai Beach and Velachery
The Chennai Corporation has commenced work on identifying the civic infrastructure requirements near the proposed metro stations across the city.
The civic body has directed officials of corporation zones in Anna Nagar, Adyar, Kodambakkam, Teynampet and Royapuram to study the deficiencies in the area lying within a radius of 500 metres of a metro station.
Chennai Metro Rail Limited in a recent meeting had asked the civic body to speed up work on creating adequate civic infrastructure such as roads, bus shelters, parking lots including multi level facilities, streetlights, signboards and storm water drains.
CMRL officials at the meeting pointed to the poor civic infrastructure around the Mass Rapid Transit System between Chennai Beach and Velachery. The poor civic infrastructure remains a hindrance to optimum use of the facility. The civic body was asked to take immediate efforts to prevent a similar condition after the Metro Rail begins operations next year in a stretch.
The Chennai Metro Rail project has two corridors — Washermenpet to Airport and Chennai Central to St. Thomas Mount. A portion of the metro alignment on Corridor-I, between Washermenpet and Saidapet, and on Corridor-II, between Central and Thirumangalam, runs as underground tunnels. Of the 36 upcoming metro stations, 20 would be underground.
The civic body has started preparing a list of pieces of lands near the upcoming metro stations for creation of parking lots.
Any multi-level facility will also be aesthetically harmonizing with the Metro Rail station. An elevated two-level station will have aesthetic all-weather roofing, an official said.
As many of the upcoming stations are yet to have world-class civic infrastructure around them, the Chennai Corporation is planning to find alternative ways to ensure that all of them have parking lots and would have facilities for feeder bus services before the stations become functional.
By the end of 2013, a passenger would be able to reach St. Thomas Mount from Koyambedu using a world-class Metro Rail service.
Before this comes into existence, the Chennai Corporation will put in place the entire civic infrastructure for a hassle-free transport network that has been designed to interlink suburban trains, MRTS services and bus stations, an official said.


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Academy of Art 2012 Spring ShowJuxtapoz // Thursday, May 31, 2012
The 2012 Academy of Art University Spring Show hosts an Open House this weekend, on Saturday, June 2, inviting the public to explore a showcase of the institution's best student works in art and design. Ongoing from 9am to 5pm, courtesy shuttles will be available from 79 New Montgomery Street to the Cannery. Admission is free, so RSVP on the next page. Preview after the jump from Juxtapoz' early visit . . .
The YokYokJuxtapoz // Thursday, May 31, 2012
Yok started his droopy eyed drawings in a salty sea shack down under in a town called Perth. He developed a passion for loopy moustaches, seaworthy beards and potato headed rad lads. Yok works from a home studio in Brooklyn, New York and has exhibited in Berlin, New York, Tokyo, Sydney, Taipei, Beijing, Singapore Bangkok and Hong Kong, As well as unoficalial displays of street art in weird parts of the world that usually involve surf.
The Work of Conrad RosetJuxtapoz // Thursday, May 31, 2012
Conrad Roset is a freelance illustrator based in Barcelona. His new muses are created with watercolor splashes and spots, more gestural to the minimal lines that we had become accustomed to. Conrad is also a graduate professor of illustration at the BAU in Barcelona. He has worked for brands like Adidas, Zara, FC Barcelona, Coca-Cola among others. Editorial Norma is currently editing a book about his work.
Bearded Ladies by Arvida Bystrom (NSFW)Juxtapoz // Thursday, May 31, 2012
The work of young photographer (like 20 years old-young) Arvida Bystrom consistently catches my eye. Whether its the dark erotic GIF's, or series of sexy bearded nudes- (hey, there's something for everyone) the appeal of her work is unavoidable. She's young and talented, and uses topics confronting gender, love, age and beauty stereotypes in her work. She has a couple editorials for Vice under her belt and with work like this, she will continue to stop me dead in my tracks.
New 'Zine from 607VISUALJuxtapoz // Wednesday, May 30, 2012
Our good friends over at 607VISUAL just released their first 'zine. We are exited to see an awesome collection images taken all over San Francisco. We recognize everything from our favorite lunch spot to the iconic Twin Peeks overlook. Even the Swoon piece that graced the cover of the May issue makes an appearance. This booklet sized full-color 24 page 'zine is perfect for your studio apartment sized coffee table.
Preview: QUAKE "26 Years of Hustlin'" @ Zerofriends, OaklandJuxtapoz // Wednesday, May 30, 2012
Alex Pardee's Zerofriends presents QUAKE: 26 Years of Hustlin', a giant retrospective of the street artist Quake's "continuous, uncompromising, vibrant pubic art by one of the Bay Area's most prolific and loyal artists," this Thursday, May 31, 2012 at Zerofriends in Oakland. The solo exhibition, on view through June 30, will include hundreds of photos taken around the world of Quake's outdoor work, alongside sketches, relics, prints, and a massive ongoing slide show, celebrating "the insanely colorful career of not only one of our favorites," Zerofriends said, "but one of our own."
IN L.A.: Augustine Kofie @ Known GalleryJuxtapoz // Wednesday, May 30, 2012
This past weekend we stopped by the opening reception of "Working An Angle", Augustine Kofie's first solo show at Known Gallery in Los Angles. The show featured 40 recent works including hand painted multiple screen prints, collage on wood as well as paintings on canvas and wood. Photos and more after the jump..
Top 20 Graffiti Artists To Know Right NowTop List // Wednesday, May 30, 2012
Everyone knows the classic, legendary names of graffiti... Dondi, Seen, Revolt, Twist, Futura, Blade, Revok, Saber, the list goes on. But what about if someone were to ask you about active writers? Maybe you are in a bar conversation and someone wants to know a few good men to check out. We compiled a list of 20 Graffiti Artists You Should Know Right Now, those who are doing interesting things, a lot of things, and are really good doing it.
"Beauty Is Embarrassing": A Documentary on Wayne WhiteJuxtapoz // Wednesday, May 30, 2012
Wayne White, featured in our June 2012 issue of the magazine, maker of the wonderful "word paintings" and the former art director of Pee-wee's Playhouse, had a documentary of his life and art, Beauty is Embarrassing, released and circulating through the film festival circuit. The film is a "funny, irreverent, joyful and inspiring documentary featuring the life and current times of one of America's most important artists, Wayne White."
Manuel Birnbacher's Civil War PortraitsJuxtapoz // Wednesday, May 30, 2012
In David Lynch's famed and well-loved television series Twin Peaks, Benjamin Horne re-enacts and reverses the outcome of the Civil War as a means of coping with a mental breakdown; by reversing the outcome of the war in the role of General Robert E. Lee, Horne is reverting the crisis that has troubled his own life. We're not sure if Manuel Birnbacher's reason for remixing and reworking these Civil War-era portraits has any similar connection to a personal crisis . . .


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Welcome to Seven Degrees of Freedom
Now in our thirteenth year!
Now in our thirteenth year!
Latest news
Sat Jan 31 12:44:15 2015 [Jaakan]
We're pleased to welcome EojRepus to the powers team, for his commitment
to current and future projects.
Mon Sep 22 17:38:31 2014 [Jaakan]
The Icon quest has been made a little easier by the addition of a
magic tally sheet which shows which ones you've collected and from where.
It's fairly easy to find. Look for the snow tree.
Wed Jul 9 23:13:37 2014 [Martyn]
Starting from today, all 7DOF bank accounts will recieve a daily interest
payment of 3% for any player that actually logs in during that time
period. If you're on the game at the time, it will tell you the amount
of interest and your new balance.
Tue Jul 8 21:19:02 2014 [Jaakan]
A new stats command, QCOMPARE has been added for you to measure each
other's performance.
Mon Jul 7 18:12:43 2014 [Jaakan]
Along with the new BOUNTY and BOUNTYLIST verbs another command has
been added, AUTOBOUNTY. If you only want to see offers above 5000
gold, for example you would type AUTOBOUNTY 5000. Enjoy.
Who is on?
57 minutes, and 22 seconds ago.