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Stamp featuring Pirates' Stargell top seller among baseball legends
A commemorative stamp featuring former Pirates slugger Willie Stargell finished as the top seller in a four-way race featuring some of the most iconic players in baseball history, the Postal Service said.
The Postal Service said it sold more than 8.2 million Stargell stamps since May — 11,320 more than one featuring former Boston Red Sox great Ted Williams, the last player to hit at least .400 in a season.
A stamp with Joe DiMaggio, the former New York Yankees outfielder who once hit safely in a record 56 straight games, finished third with sales of almost 8.1 million. Customers bought 7.8 million stamps featuring former Cleveland Indians outfielder Larry Doby, the first black player in the American League.
Stargell, nicknamed "Pops," hit 475 home runs and led the Pirates to two World Series titles in his Hall of Fame career from 1962-1982. Stargell died in 2001.
The Postal Service's Stamps Batted In race began in earnest July 20 and concluded with the end of the World Series on Oct. 28.
Tad Kelly, a Postal Service spokesman in Pittsburgh, said Stargell came from behind to win. Williams and DiMaggio were far ahead of the pack from late May through July 20, when customers could order the Major League Baseball All-Stars Forever stamps before they went on sale to the general public.
"It's another feather in the cap for Pittsburgh. It shows the way we support our sports teams and revere our sports legends," Kelly said.
Tom Fontaine is a staff writer for Trib Total Media. He can be reached at 412-320-7847 or firstname.lastname@example.com.
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Fi News #138: Cardinal Burke Blesses Friary Statue of Blessed John Dun Scotus
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Ave Maria! On December 12th, 2012, His Eminence Raymond Leo Cardinal Burke blessed a newly unveiled statue of Blessed John Dun Scotus in front of the Friary Chapel at the Shrine of Our Lady of Guadalupe in La Crosse, WI. Following the ceremony His Eminence gave an account of the Blessed's life and the great theological contribution he made to the Church, especially in regards to the declaration of the Immaculate Conception of the Blessed Virgin Mary. To read the full text of the Cardinal's talk go to http://s3.amazonaws.com/airmaria/finews/cardinal_burke_scotus.pdf Ave Maria! To Download Audio go to http://airmaria.com?p=33056
Bl John Duns Scotus Cardinal Raymond Leo Burke La Crosse Statue
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Hotel Centrale
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TravelPod Member ReviewsHotel Centrale Bellagio
If you stay in Bellagio and don't want to spring for the lake-front resort stay, this is your place. Such friendly hosts, a clean room with a view, and bargain-priced (for Bellagio, anyway). Nothing bad to stay about Hotel Centrale, it was wonderful all they way around.
This review is the subjective opinion of a TravelPod member and not of TravelPod.com.
Historical Traveler Reviews Hotel Centrale Bellagio
Worth a long detour
I found this hotel by chance. My business took me to the Lecco area and I decided to make a detour to Bellagio. I found the hotel very charming and the owners just wonderful. This isn't just a hotel: it's a place where you can truly make yourself at home.
Can only agree with the other reviews
I can only agree with wat was written in the earlier reviews - originally we had wanted to book in Metropole, but that was already closed for the winter season. Based on the good reviews on this site, we ended up in Centrale, and we certainly didn't regret it: we had a nice room on the 3rd floor with some view on the lake - price/quality I would say certainly (****), very nice breakfast with lots of choice and very friendly staff. We would certainly consider going back for our next holidays in the region.
Absolutely Wonderful
A fantastic Hotel with such friendly owners and staff. They really care about their guests - so rare these days. They mainly get new visitors by word of mouth and that tells you everything. The rooms are small but clean and have character (larger folks may find it a little difficult in the quite small shower room). The hotel has a quality feel about it and is very Italian. There is an elevator. A nice garden at the front.
The hotel is located on a narrow pendestrian pathway which leads from the top of Bellagio to the Lake. This could make it tricky for those with walking difficulties.
We had a view of the Lake from the room (if you stuck your head out of the window - but a clear view nevertheless).
You can drive close to the Hotel and park in a small car park near the Church to unload bags - but it is tricky as you have to drive down the pedestrian pathways. Parking is best on street and there are spaces depending on when you arrive. Prepare to walk with your cases though.
Plenty of local bars close by and even Premiership football live on TV in the Sports Bar near the Church.
There did not seem to be any travel agent links so try direct at ---- Euro 110 per night including breakfast.
Would definately stay again.
Highlight of my two week trip to Italy
I recently got married in Mennagio and my wedding party stayed in the Hotel Centrale during that time. The owners of the hotels went out of thier way on many occasions to make us feel truly at home. We forgot to get the flowers for my wife and so they drove us to the local florist and helped us pick out a bouquet. They called ahead for restaurant reservations for us and even aranged a table for 8 in a popular restaurant that doesn't normally take reservations. Most importantly when it started pooring down rain on our way to the wedding hall they provided the entire group with umbrellas and even a raincoat to cover my wife's dress so it would not get wet. The weather eventually cleared up and my appreciation for thier kindness will not fade. They even had a bottle of bubbly and a bowl of strawberries waiting for us when we got back. The rooms are charming and bright and you can not beat the location. Be prepared for the walk up the long flight of stairs from the ferry dock, but believe me it will be worth all of your efforts in the end. Other hotels may have the views but Centrale has the most caring staff and attracts the kind of friendly people who will make your stay in Bellagio memorable. Highly recommended.
Superb Service and Truly Cozy
We spent eight days of our honeymoon in and around Lake Como and we cannot forget Hotel Centrale. We found it on a whim; we already had lodgings in Varenna, but we found Hotel Centrale so much more charming and honeymoon-worthy. The owner treated us like family and the rooms we super clean, yet cozy. The price was extremely reasonable, and yet it felt nothing like other "budget" hotels that we have learned to be afraid of. As regards to value, it can't be beat!
A Home Away from Home
This hotel was wonderful. It was exceptionally clean with air conditioning and nice bathroom. We had a room on the third floor with a view of the lake. Giacomo and his family were warm and hospitable. We truly enjoyed our time here and our talks with Giacomo. He recommended wonderful restaurants and happily answered all of our questions. We hope to return again soon. For 100 Euro a night, you can't beat it!
Charming family hotel in Bellagio
We recently stayed at Hotel Centrale in Bellagio. It is in a very central location (hence the name!) but more importantly is a charming family-run place. We were welcomed by Giacomo and his family and he was very happy to suggest where to eat and also wanting to know if we were happy if we went to the places that he recommended. (We were). Our room was clean, quiet and had a partial view of Lake Como (what more can you want?). There was also air conditioning and tv which is less important to us but nice to have. Giacomo said the top floor rooms are even better but unfortunately were booked out. We enjoyed breakfast each morning in the lovely breakfast room with fab views of the Lake. Breakfast was very substantial with fantastic coffee. The hotel also has a beautiful courtyard garden. If you enjoy staying in charming family hotels I highly recommend this place.
Charm with a great price! :-)
This family owned hotel had a ton of old world charm with a touch of newness that made our stay in Bellagio a wonderful experience. From the neatness of the rooms to the expansive breakfast buffet, our hosts made sure our stay was a delightful one. We look forward to making a return visit soon!
Don't pass this place by!
We cannot say enough to express the wonderful time we had while staying here. The warmth, the kindness, the incredible service straight from the heart was uncomparable to any place we've stayed. Giacamo and his beautiful family made us feel right at home in their immaculate accomodations. The breakfast and never ending cappucino cups were most comforting. Giacamo gave us plenty of time and history along with local recommendations. We would recommend this place to anyone, and look forward to a return visit to see our "new friends".
George and Kim Wilson
Sebastopol, California
Perfectly charming
This was a wonderful experience. The hotel is family owned and newly renovated, right in the heart of Bellagio. The room was cozy and while nothing fancy, was spotless and comfortable. It was old world with a bit of the new (satellite TV) The service, provided by the owner and his family, was humane. On top of everything else, there was a great breakfast and cappucino every morning. And for the price it cannot be beat. We would gladly go back.
TripAdvisor Reviews Hotel Centrale Bellagio
Travel Blogs from Bellagio
Italy at last
6 thousand toll booths later we exit at Como the city and drive along the lake towards Bellagio. What a gorgeous drive, reminds me of Lake Tahoe in a way, but is scattered with red roofed buildings in clusters along the cliffs and the shore. We arrive in hopes of a free room at a rec of RS, Hotel Centrale. We are in luck again! Such a cute little …
Come to Como
... the lunch hour began. The builders came in in hordes. Seated, supplied with bread and wine followed by pasta and then time to get back to work. We moved on and later found a great pizza place. Pizzas are good here (unlike Rome). We then followed Fabio's advice and caught the ferry into Como. We spent the afternoon walking about the main part of the city - lovely old buildings, huge Duomo (surprise!!), partially unfinished waterfront, shops ...
Sparkling Lights in Lugano
We arrived in this beautiful city at dusk so we weren't able to get the most out of it. Stayed there only for one hour and we spent that time in Parco Civico capturing the flickering lights of the city surrounded by mountains. The view from the shores of Lake Lugano was fascinating. It's truly a must-visit city and I'd love to go back and see it in broad daylight. ...
Bergamo and the Alps
... It ended up being a winding road up the mountain curving around the entire thing. What we thought would take about 10 minutes, probably took a half an hour. However, we finally arrived at the castle, which was beautiful and had amazing views as well.
After spending time in the old city, we decided to go back to the lower city for dinner (we were very tired due to lack of sleep). We grabbed some pizza ...


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The White Towel
Canucks prospects win 5-4 to end tournament.
Outshot, but not outscored, the Vancouver Canucks beat the Calgary Flames 5-4 to end their prospect's tournament with a 2-2 record. Vancouver was outshot 49-20 but goalie Eddie Lack, nicknamed The Stork, was tremendous. So was Jordan Schroeder who had … Continue reading
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Wednesday's teenage goalie heading back to Jr.
PENTICTON _ Michael Houser hadn't even turned 18 yet when he weighed in for Canucks' prospects physicals last week _ his birthday was on Saturday. But the kid looked really solid against Anaheim's more-talented lineup Wednesday, including Cam Fowler and three … Continue reading
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Morrison still playing the waiting game
Former Canuck Brendan Morrison is still skating with the vets in informal workouts, but that will cease on Friday when training camp officially begins for the NHL club. Morrison, an unrestricted free agent, is still looking for a team to … Continue reading


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Click here to print the obituary below
Esther Downs Kroll
Esther Downs Kroll, 89, of Forty Fort, died Saturday evening, April 6, 2013, at St. Luke's Villa, Wilkes-Barre, where she was a guest.
She was born in Forty Fort on Oct. 22, 1923, and was the daughter of the late Gwilym I. and Anna Blower Downs.
She was a graduate of Hanover Area High School. For several years she studied painting with an instructor throughout Wyoming Valley, and also attended local colleges studying art and literature. She was a member of Wyoming Valley Art League, which many times she exhibited her work. With her love of history many of her watercolors were of historical sites that she had visited throughout the United States. She created beautiful needle work that she shared with family and friends. She had a love for reading and books that she treasured.
Mrs. Kroll was a member of Forty Fort United Methodist Church. She spent 10 years as a leader for Cub Scouts and Girl Scouts, she also was a volunteer for the United Way and the American Red Cross.
She was preceded in death by her husband, Fred J. Kroll, in 1981; and a brother, Robert I. Downs.
She is survived by her son, Gwilym (Guy) and his wife, Noreen, Kingston; her daughter, Kathleen, Forty Fort, whom she resided with until her illness; two grandsons, Thomas and his wife, Geana, Harrisburg; and Michael James, Kingston; two great-grandchildren, Gabrella and Jackson.
The family extends thanks to the staff at St. Luke's Villa for their kindness and Dr. William Boyle for his care.
Private services will be held at Hugh B. Hughes & Son Inc. Funeral Home, 1044 Wyoming Ave., Forty Fort. The interment will be in Evergreen Cemetery, Shavertown.
In lieu of flowers, memorial contributions can be made to The Lands at Hillside Farms, 65 Hillside Road, Shavertown, PA 18708.
For information or to send the family an online message of condolence, you may visit the funeral home website at
Published in Citizens' Voice on Apr. 8, 2013


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FULL RESOLUTION - 800x1146
News » Published months ago
Is this Teresa Palmer the Actor?
Share your thoughts on this image?
10th September 2014 @ 08:44am
Teresa Palmer says she is not going to let talk of nude pictures have a negative impact on her happy life
17th June 2014 @ 08:27pm
Teresa Palmer will join Edgar Ramirez and Luke Bracey in the cast of the Point Break remake
17th April 2014 @ 06:22am
Mark Webber wrote the screenplay for The Ever After with wife Teresa Palmer and he has spoken about working with his wife on the project


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Beduin protest march: 'If it is not good for us, it is not good for the Jews in the Negev'
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07/27/2012 18:10
IAF F-15s refueling midflight 390 (R).
(photo credit:Baz Ratner / Reuters)
A series of malfunctions led the Israel Air Force over the weekend to suspend midair refueling exercises as well as training flights for its fleet of older-model F-16s.On Friday, during a routine midair training flight, the hose disconnected from an F-16I - the IAF's most-advanced combat aircraft - causing light damage. The IAF decided to suspend the exercise and to land both the F-16 and the Boeing 707 refueling tanker.Midair refueling is considered a critical capability for the IAF as it faces long-range missions, including a possible strike against Iran's nuclear facilities.The midair refueling malfunction came after IAF commander Maj.-Gen. Amir Eshel decided earlier in July to ground the force's fleet of F-16 C/Ds, known as Barak in the military.The decision was made after suspicions arose that the heavy heat was causing damage to the aircraft's single engine.Last year, the IAF began an upgrade program for its Barak F-16s including the installation of new avionics and a new mission-debriefing system. Aimed at increasing the aircraft's lifespan, the IAF has also decided to upgrade the F-16s flight-control system as well as its central display unit and the aircraft have also been fitted with new high-resolution screens.Pilots of the Barak aircraft are also now equipped with Elbit's display and sight helmet system, which enables pilots to aim their weapons simply by looking at their targets.
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Senior Member
than you must be playing a terrible darius! darius is most op!!! everything about him is op!
The Codyman
Yeah nothing in ranked yet, just normals for now, but I have seen quite a few, usually not as bad as a jax imo.
Also, what I am doing wrong with darius ? i am not so good with him.
Dracos Kasurani
It is not needed but if you want to piss off the other team take him, I believe they should limit the number of shroom on the map.
Wandering Sentry
Junior Member
Limit the shrooms? They count for his ultimate but are not that powerful alone, so why limit them?
teemo without ult would be bad, I went 7-0 with teemo before my first loss, and people say ap teemo is bad because all he has is a lil burst.
The Bushmaster
Just had 2 troll teemos in a row, so I'm pretty partial the other way. I say damn the teemo, and damn the mushrooms. I see how his shrooms would help, but all it takes is a tank walking into key locations to pop them off or using one of the little CV items they implemented to chop them all down. That is, of course, if they're really bothering you that much. If you know teemo's got the jungle all fungus'd up, plow the ever-loving hell out of the lanes. If he's got the lanes, gank from the jungle. If he's got both, I don't know why you left teemo alone long enough to put down that many shrooms.
The shrooms purpose serves as vision and map awareness, not damage. This crucial vision can easily help win games
Joe Krake
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Zachary Rodgers | September 27, 2007 | Comments
Female-focused publishers are really good at co-opting each other's ideas
Female-focused publishers are really good at co-opting each other's ideas. It wasn't long after Glam Media became the first notable smaller publisher to represent an extended network of sites that iVillage began doing the same. And this week, with the acquisition of "social shopping" site ShopStyle, Sugar Publishing has obtained new e-commerce and product discovery capabilities of the sort both iVillage and Glam offer.
In a video interview with AllThingsDigital's Kara Shwisher, publisher Brian Sugar describes the structure and strategy of the business, which has been renamed Sugar Inc. Along the way he takes a bunch of subtle and not-so-subtle swipes at rivals Gawker Media and Glam.
"Snarky's just sort of a trendy thing that eventually goes away," he tells Swisher, dissing Gawker without mentioning it by name (though Swisher had earlier referred to him as the "sweet Nick Denton"). "People magazine has a style we like. We model after them."
And in a blatant attack on Glam Media, Sugar tells Swisher he "really hates" sites that roll up comScore data from an extended ad network to "say they're bigger than they really are." Call it artificial sweetener.
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Lingyun Zhou and Ying Liu contributed equally to this work.
Cardiovascular Biology
The Effect of the Gly139His, Gly143His, and Ser142His Mouse Heme Oxygenase-1 Mutants on the HO Reaction In Vivo and In Vitro
Article first published online: 16 NOV 2010
DOI: 10.1002/ar.21284
Copyright © 2010 Wiley-Liss, Inc.
The Anatomical Record
Volume 294, Issue 1, pages 112–118, January 2011
Additional Information
How to Cite
Zhou, L., Liu, Y., Zou, C., Ma, N., Hui, Y., Lv, G., Zhang, H., Zhou, H. and Gao, X. (2011), The Effect of the Gly139His, Gly143His, and Ser142His Mouse Heme Oxygenase-1 Mutants on the HO Reaction In Vivo and In Vitro. Anat Rec, 294: 112–118. doi: 10.1002/ar.21284
Publication History
- Issue published online: 13 DEC 2010
- Article first published online: 16 NOV 2010
- Manuscript Accepted: 6 SEP 2010
- Manuscript Received: 14 APR 2010
Funded by
- Major State Basic Research Development Program of China. Grant Number: 973 Program (No. 2009CB521702);
- Heilongjiang Province Foundation for Returnees. Grant Number: LC06C21
- Abstract
- Article
- References
- Cited By
- heme oxygenase-1;
- Gly139His mutant;
- Gly143His mutant;
- mouse
- Top of page
- Abstract
- Acknowledgements
Heme oxygenase-1 (HO-1), which catalyzes the degradation of heme to iron, carbon monoxide, and biliverdin, performs a cytoprotective function. Previous studies on the crystal structure of the human and rat HO-1 in complex with heme showed that Gly139His (G139H) and Gly143His (G143H) mutants have no HO activity. In the present study, we reported the effect of the G139H, G143H, and Ser142His mutants of mouse HO-1 on the HO reaction in vivo and in vitro. In vitro, of the mutant transfectants, only Ser142His catalyzed degradation of heme, retaining 31.7% of the wild-type mouse HO-1 activity, whereas G139H and G143H mutants exhibited no activity. In vivo, only Tg HO-1 G143H females presented with anemia, enlarged spleen and tissue iron overload, which was similar to HO-1−/− mice. The results suggested the critical role of Gly139 and Gly143 in maintaining HO-1 activity in vitro and the critical role of Gly143 in maintaining HO-1 activity in vivo. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.
- Top of page
- Abstract
- Acknowledgements
Heme oxygenase-1 (HO-1) catalyzes the rate-limiting step in the oxidative degradation of heme to release iron, carbon monoxide, and biliverdin (Tenhunen et al.,1968). Due to the potent antioxidant activity of biliverdin and bilirubin, and to the cytoprotective actions of carbon monoxide on vascular endothelium and nerve cells, it is widely accepted that upregulation of HO-1 causes beneficial effects (Stocker et al.,1987; Verma et al.,1993; Brouard et al.,2000; Colle et al.,2008; Mancuso and Barone,2009; Ryter and Choi,2009; Gozzelino et al.,2010). In contrast, the lack of, or inhibition of, HO-1 generally accelerates growth retardation, anemia, and tissue iron deposition, as seen in HO-1-deficient humans and mice (Poss and Tonegawa,1997a,b; Kapturczak et al.,2004; Zhao et al.,2009).
There are two isoforms of HO; HO-1 and HO-2. A third isoform, known as HO-3, has been described in the rat brain; however, no homolog of this gene is found in either humans or mice (Hayashi et al.,2004). HO-1 is inducible by various substances and is highly expressed in the spleen and liver. HO-2 is constitutively expressed in the brain, testis, and vascular systems. HO enzymes are membrane-bound proteins but are water soluble. Truncated forms of HO-1, which lack the C-terminal 23-amino acid membrane anchor, have been used to obtain crystal structures of both the human and rat enzymes and their catalytically active versions have been expressed in Escherichia coli (Wilks and Ortiz de Montellano,1993; Liu et al.,2000; Sugishima et al.,2000). The truncated rat and human HO-1 crystal structure shows that the heme pocket is primarily formed by two helices, one on the proximal and the other on the distal side of the heme molecule. The proximal helix consists of Leu13 to Glu29, including the proximal ligand of heme, His25. The distal helix consists of Leu129 to Met155 and bends at Leu141 and Ser142 (Wilks and Ortiz de Montellano,1993; Liu et al.,2000; Sugishima et al.,2000; Lad et al.,2005). In these studies, the crystal structure of the truncated rat and human HO-1 suggested that Gly139 and Gly143 interact directly with heme. Gly139His (G139H) and Gly143His (G143H) mutants of HO-1 bind heme but have no HO activity.
As the amino acid sequence in the distal helix is well conserved among animal species (Maines and Gibbs,2005), we constructed and expressed G139H, G143H, and Ser142His (S142H) mutants of mouse HO-1 (mHO-1) to determine the importance of the three conserved residues in mHO-1-catalyzed heme degradation in vivo and in vitro.
- Top of page
- Abstract
- Acknowledgements
Preparation of Plasmid cDNAs
We prepared pCAGG G139H mutant mouse HO-1 cDNA (pCAGG-mHO-1-G139H), pCAGG S142H mutant mouse HO-1 cDNA (pCAGG-mHO-1-S142H) and pCAGG G143H mutant mouse HO-1 cDNA (pCAGG-mHO-1-G143H) constructs using the QuikChange site-directed mutagenesis kit (Stratagene). In brief, a mHO-1 cDNA (provided by Professor Osamu Nakajima, Yamagata University, Japan) was inserted into the pCAGGS vector (Niwa et al.,1991) at the EcoRI site (pCAGG-mHO-1), which expresses the mHO-1 cDNA under the control of the chicken β-actin promoter. pCAGG-mHO-1 was used as a template to mutate Gly139, Ser142, and Gly143 of mHO-1 protein to His. Transformants were screened by restriction digestion and confirmed by sequence analysis. Plasmid purification, sequencing, subcloning, and bacterial transformations were carried out by standard procedures (Sambrook and Russel,2001).
Transient Transfection of Wild-Type mHO-1 and/or Mutant mHO-1 in Eukaryotic Cells
QT6 quail cells were maintained in Dulbecco's Modified Eagle's Medium supplemented with 10% (vol/vol) heat-inactivated fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate (all from Sigma). First, transient transfection was performed in 10-cm dishes by calcium phosphate precipitation with 5 μg of pCAGG-mHO-1 plasmid. HO activity was then determined under conditions of 3, 7.5, 15, and 25 μM heme to determine which final heme concentrations were excessive and insufficient for 5 μg of pCAGG-mHO-1 plasmid transfection. Second, transient transfection was performed in 10-cm dishes by calcium phosphate precipitation with 5 μg of pCAGG-mHO-1-G139H, 5 μg of pCAGG-mHO-1-S142H, or 5 μg of pCAGG-mHO-1-G143H plasmid in the presence or absence of 5 μg of pCAGG-mHO-1 plasmid. HO activities were then determined under conditions of excessive heme. Third, transient cotransfection was performed in 10-cm dishes by calcium phosphate precipitation with 5 μg of pCAGG-mHO-1 plasmid, along with 3, 7.5, 15, or 25 μg of pCAGG-mHO-1-G139H or pCAGG-mHO-1-G143H plasmid. HO activities were then determined under conditions of insufficient heme. The pCAGG plasmid was introduced in some reactions to maintain a constant quantity of DNA. pCAGG-EGFP plasmid (provided by Professor Osamu Nakajima, Yamagata University, Japan) was introduced as an internal control. Each transfection replicated in at least three separate experiments.
Transient transfection of COS7, NIH3T3, and Hepa1 cells was performed in 10-cm dishes using lipofection (Invitrogen) with 5 μg of pCAGG-mHO-1-G139H, 5 μg of pCAGG-mHO-1-S142H, or 5 μg of pCAGG-mHO-1-G143H plasmid in the presence or absence of 5 μg of pCAGG-mHO-1 plasmid. HO activities were then determined under conditions of excessive heme.
HO Activities in the Transformants
HO activities in the transfected cells were determined by measuring the formation of bilirubin as described previously (Lincoln et al.,1988). Briefly, cells were homogenized on ice with 0.25 M sucrose, 20 mM Tris-HCl (pH 7.4) buffer. Homogenates were mixed with 5 mM deferoxamine, 25 μM hemin (in the second transient transfection experiment), or 7.5 μM hemin (in the third transient cotransfection experiment), 15 μM bovine serum albumin, 1 mM NADPH, 0.1 M potassium phosphate (pH 7.4), and purified biliverdin reductase and cytochrome P450 reductase (provided by Professor Tadashi Yoshida, Yamagata University, Japan). After incubation at 37°C for 10 min, any insoluble material was removed by centrifugation and supernatants were analyzed for bilirubin concentration by reverse phase high-performance liquid chromatography.
Animal experiments were approved by the Animal Care Committee of Harbin Medical University. Care and handling of the animals were in accordance with National Institutes of Health guidelines. Mice housed under identical conditions were allowed free access to a standard diet and to tap water, with a 12-hr light: 12-hr dark cycle.
Generation of G139H, S142H, and G143H Mutant mHO-1 Transgenic Mice
The transgenes, isolated from the pCAGG-mHO-1, pCAGG-mHO-1-G139H, pCAGG-mHO-1-G143H, and pCAGG-mHO-1-S142H were separately injected into C57BL/6 fertilized eggs for the generation of transgenic mice, as described previously (Zhou et al.,1998). The HO-1 transgenic allele was identified by analysis of tail DNA by reverse transcription-polymerase chain reaction (RT-PCR), using an upper primer from the β-actin promoter (5′-GCCTTCTTCTTTTTCCTACAGCTC-3′) and a lower primer from the mHO-1 cDNA (5′-GGCATGCTGTCGGGCTGTGGAC-3′).
Reverse Transcription-Polymerase Chain Reaction
Total RNA was extracted from the livers of 3.5-month-old mice using Isogen (WAKO), according to the manufacturer's instructions. RNA concentration and purity was determined spectrophotometrically at 260 and 280 nm. First strand cDNA was synthesized using superscript II (Invitrogen).
Blood Cell Counts and Histology
Blood was obtained by retro-orbital sampling, and blood cell counts were determined using a veterinary hematology analyzer (Kohden, Japan). For histology, tissues were fixed in 10% buffered formalin and paraffin embedded. Sections (4-μm thick) were stained with Prussian blue to detect ferric iron (Nakajima et al.,1999). Counterstaining was performed with nuclear fast red for 10 sec to maximize the detection of iron.
Serum Iron Parameters
Mice were bled retro-orbitally and 200 μL of serum from each animal was used for analysis of iron and total iron-binging capacity (TIBC) using a kit from Sigma.
Statistical Analysis
Results are presented as means ± SEM. Statistical evaluation was performed with an unpaired Student's t test for two groups or one-way ANOVA for multiple groups. Differences were considered as significant at P < 0.05.
- Top of page
- Abstract
- Acknowledgements
Activities of the G139H, S142H, and G143H mHO-1 Mutants in QT6 Cells
To examine the activities of G139H, S142H, and G143H mHO-1 mutants in eukaryotic cells, transient transfection of QT6 cells was performed with wild-type or mutant HO-1 cDNA. As shown in Fig. 1A, the parent and mock transfectant cells did not show any notable HO activity. The wild-type HO-1 transfectant exhibited 710.01 pmol bilirubin/mg protein/min enzymatic activity. The S142H mutant transfectant exhibited 224.91 pmol bilirubin/mg protein/min enzymatic activity, while the G139H and G143H mutant transfectants did not show any notable elevation of enzymatic activity. In addition, the cells cotransfected with the wild-type mHO-1 and mutant S142H mHO-1 cDNA displayed 1042.23 pmol bilirubin/mg protein/min enzymatic activity, which roughly equates to a combination of 710.01 and 224.91 pmol bilirubin/mg protein/min. Compared with the wild-type mHO-1 transfectant, the cells cotransfected with wild-type and mutant G139H or G143H mHO-1 cDNA did not show any elevation of HO enzymatic activity. We observed similar results using transient transfection of COS7, NIH3T3, and Hepa 1 cells. The G139H and G143H mutant transfectants did not show any notable elevation of HO enzymatic activity. The S142H mutant transfectant exhibited one-third of the enzymatic activity of the wild-type HO-1 transfectant. COS7, NIH3T3, and Hepa 1 cells cotransfected with wild-type and mutant G139H or G143H mHO-1 cDNA did not show any elevation of HO enzymatic activity compared with the wild-type mHO-1 alone transfectant (data not shown). These results indicated that mutant G139H and G143H mHO-1 lacked catalytic activity in eukaryotic cells, and that mutant S142H mHO-1 had decreased catalytic activity in eukaryotic cells. On the other hand, the results suggested that mutant G139H or G143H mHO-1 and wild-type mHO-1 cotransfectants did not contain elevated HO activities compared with wild-type mHO-1 alone transfectant.
Previous studies on the crystal structure of the truncated rat and human HO-1 suggested that G139H and G143H mutants of HO-1 had no activity but did bind heme. In the above experiments, HO activities were measured in a common reaction buffer containing excessive heme. Under these conditions, it was unclear whether G139H and G143H mHO-1 mutants bound to heme or not. To clarify this, we first demonstrated that for 5 μg of wild-type mHO-1 DNA transfection, 7.5 μM hemin in the reaction buffer of HO activity was insufficient to allow maximum HO activity (Fig. 1B). The cells were then cotransfected with the wild type and increasing doses of mutant G139H or G143H HO-1 cDNA and HO activities were measured under conditions of 7.5 μM heme. As shown in Fig. 1C, cotransfectants displayed decreasing HO activity in a dose-dependent manner. As compared with mutant G139H and wild-type HO-1 cotransfectants, the decrease in HO activity in mutant G143H and wild-type HO-1 cotransfectants was more obvious. These results suggested that G139H and G143H mutant mHO-1 could bind heme, and that cotransfection with mutant G139H or G143H mHO-1 and wild-type mHO-1 might decrease HO activity under conditions of insufficient heme.
Generation of G139H, S142H, and G143H mHO-1 Mutant Transgenic Mice
In vivo heme concentration is insufficient for HO activity reaction. According to the in vitro results, the overexpression of G139H and G143H mHO-1 mutants in vivo was expected to reduce endogenous HO activity. Thus, the phenotype of the mutant HO-1 overexpressing mice might resemble HO-1-deficient mice. Therefore, we generated four founder lines of G139H, three founder lines of S142H, and three founder lines of G143H mutant HO-1 transgenic mice (Tg HO-1 G139H, Tg HO-1 S142H, and Tg HO-1 G143H). RT-PCR analyses of the transgene-derived HO-1 mRNA expression in the livers among the established lines of Tg HO-1 G143H mice showed extremely high expression in two lines (H1 and H2 lines) and low expression in one line (Fig. 2A). RT-PCR analyses of the transgene-derived HO-1 mRNA expression in the livers among the established lines of Tg HO-1 G139H and Tg HO-1 S142H mice showed high expression in two lines and low expression in the other two lines, and high expression in one line and low expression in two lines, respectively (data not shown). No increase in levels of HO-1 mRNA was detected in the livers of the H1 line of Tg HO-1 G143H mice compared with high-expression lines of Tg HO-1 G139H and S142H mice (Fig. 2B).
Partial Prenatal and Natal Lethality Among Tg HO-1 G143H Males From H1 and H2 Lines, and Splenomegaly, Anemia, Serum Iron Reduction in Tg HO-1 G143H Females From H1 and H2 Lines
Of 50 offspring, 12 male and 13 female pups from the low line expressed Tg HO-1 G143H, which was in accordance with the Mendelian ratio (Table 1). Of 48 offspring, five male pups from the H1 line and four male pups from the H2 line expressed Tg HO-1 G143H (Table 1). Unexpectedly, these nine Tg males had died within 1 day (Table 1). The birthrate of Tg HO-1 G143H males in two high-expression lines was significantly lower than that in the low-expression line, whereas the birthrate of Tg HO-1 G143H females in the two high- and low-expression lines was in accordance with the Mendelian ratio. Tg mice mated between Tg HO-1 G139H/Tg HO-1 S142H and wild-type mice yield the expected Mendelian ratio. This remainder of the report focuses on the phenotype of the mutant Tg mice, while the basis of the prenatal and natal lethality of Tg HO-1 G143H males will be described in detail elsewhere.
|12 (13)/50
|5 (12)/48
|4 (13)/48
|1 Day
|12 (13)/50
|0 (12)/43
|0 (13)/48
|1 Year
|12 (13)/50
|0 (12)/43
|0 (13)/48
Tg HO-1 G143H females were slightly smaller than sex-matched wild-type littermates from 24 weeks-of-age to middle age (Fig. 3A) but were otherwise indistinguishable. To assess the possible pathological consequence associated with the wasting in Tg HO-1 G143H females, we first anatomized some 12-month-old mice from the H1 and H2 lines. We observed enlarged spleens in all examined Tg HO-1 G143H females (Fig. 3B). Associated with the splenomegaly was an anemia, as shown in the analyses of blood hemoglobin concentration and mean cell volume, but no inflammatory disease, as shown in the analysis of white blood cell counts (Table 2). This anemia was microcytic and hypochromic, similar to hypoferremia caused by iron deficiency. We then analyzed serum iron levels and TIBC in 12-month-old Tg HO-1 G143H females from the H1 line. As predicted, the serum iron value was reduced and the TIBC was elevated in Tg HO-1 G143H females compared with sex-matched wild-type littermates (Table 3). These results indicated that heme deficiency was caused by transgenic overexpression of G143H mutant mHO-1. That is, mutant G143H mHO-1 bound heme, which caused endogenous heme deficiency, but mutant G143H mHO-1 lacked enzymatic activity according to the results in QT6 cells, which caused serum iron reduction. We did not observe any wasting, enlarged spleen, anemia, and reduction of serum iron levels in Tg HO-1 S142H mice or Tg HO-1 G139H mice, which suggested Gly143 was critical in interacting directly with heme in vivo.
|WBC (103/μL)
|RBC (106/μL)
|Hb (g/dL)
|MCV (fL)
|7.49 ± 0.94
|8.49 ± 1.03
|14.5 ± 5.69
|51.93 ± 5.13
|Tg♀ (N = 10)
|6.31 ± 1.27a
|8.78 ± 0.76
|11.9 ± 1.71b
|43.03 ± 4.62b
|Serum iron (μg/dL)
|TIBC (μg/dL)
|153.67 ± 25.33
|337.23 ± 14.62
|Tg♀ (N = 8)
|136.73 ± 23.91a
|397.61 ± 24.11a
We noticed that the phenotype of Tg HO-1 G143H females from the H1 line was similar to that of HO-1 knockout mice, which developed anemia, low serum iron levels, but with high iron loading in tissues (Poss and Tonegawa,1997b). Examination of organs for iron by Prussian blue staining also showed hepatic and renal iron deposition in 12-month-old Tg HO-1 G143H females (Fig. 3C–F).
- Top of page
- Abstract
- Acknowledgements
The crystal structure of the human and rat HO-1 in complex with heme has been solved. The possible role of G139 and G143 in the HO reaction is described in these studies. Of the G139 and G143 mutants investigated, G139H and G143H mutants have no HO activity, which suggest the importance of Gly139 and Gly143 in maintaining the appropriate environment for the HO reaction. In the present study, we did not determine the crystal structures of mHO-1-heme complexes, but we report the effect of the G139H, G143H, and S142H mHO-1 mutants on the HO reaction in vivo and in vitro.
In vitro, only S142H catalyzed degradation of heme to biliverdin and retained 31.7% of the wild-type HO activity, whereas the G139H and G143H mutants exhibited no activity, which demonstrated that Gly139 and Gly143 are necessary for HO activity. The results in eukaryotic cells are comparable with those derived from the study of the crystal structure. Under conditions of insufficient heme, cotransfection of wild-type and mutant G139H or G143H mHO-1 showed HO deficiency, which suggested that G139H and G143H mutants were in competition with wild-type mHO-1 to bind heme. We therefore expected that mutant mHO-1 G143H or G139H overexpression in vivo might reduce endogenous heme, mimicking HO deficiency.
In our studies, Tg HO-1 G143H females are presented with anemia, enlarged spleens, and iron overload. We did not observe such a phenotype in Tg HO-1 G139H mice or Tg HO-1 S142H mice, which suggested that Gly143 was more critical in maintaining the HO reaction in vivo. The phenotype shown in Tg HO-1 G143H females constituted the most obvious similarity with HO-1−/− mice (Poss and Tonegawa,1997b). Thus, the Tg HO-1 G143H females described here might represent an animal model of HO deficiency disorders. However, HO-1−/− mice contracted a progressive chronic inflammation, as demonstrated by high peripheral white blood cell counts and lymph node CD4+:CD8+ T-cell ratios, unlike Tg HO-1 G143H females who had normal white blood cell counts. The simplest explanation for these results is that HO-1−/− mice have a defect in the protective effects caused by the products of heme degradation, while Tg HO-1 G143H females have deficient HO activity whilst retaining some protective effects. Interestingly, the appearance of pathological iron overload in the liver and kidney of Tg HO-1 G143H females coincided with the diminishing of serum iron levels, similar to HO-1−/− mice. The explanation for tissue iron retention in the HO-1−/− studies relies on the requirement of HO-1-mediated heme degradation as a part of physiological iron homeostasis, that is, HO-1 activity not only contributes to release of iron but is also required for iron reutilization, although it is still unclear how iron is reutilized by HO-1. We also observed that iron loading in livers and kidneys of HO-1−/− mice, which showed no HO activity, was conspicuous compared with Tg HO-1 G143H females, which showed HO deficiency. This demonstrated that HO has an important role in iron reutilization. Thus, Tg HO-1 G143H females appear to provide another useful model for the study of mammalian iron metabolism, especially for the study of the metabolism of iron reutilization involving HO-1.
Tg HO-1 S142H and Tg HO-1 G139H mice did not show any wasting, enlarged spleen, anemia, or reduction of serum iron levels, which suggested that Gly143 was more critical in maintaining the HO reaction in vivo.
- Top of page
- Abstract
- Acknowledgements
The authors thank Osamu Nakajima and Tadashi Yoshida for their help with valuable advice concerning the selection of mutations.
- Top of page
- Abstract
- Acknowledgements
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text:
Weird/What do you think?
My husband got a 1 am phone call on his cell phone, telling him he 'was going to go to hell for what he's done'. My husband hung up and went back to sleep. The number was blocked. He thought it was either his ex or his daughter. He has cut off all contact with them due to all the false accusations and the animosity. They also moved 1,000 miles away making contact difficult in these circumstances.
What are they talking about? What is going on here? What does she want/expect? Any ideas/suggestions to get her to move on and accept responsiblity for her own life? She's been living with a guy for 7 years and she married him last fall.
The current situation:
We have been in almost constant litigation for the past 8 years, she has gotten generous child support awards. She has been trying to put my husband (her ex) in prison for the past 8 years, ever since he got involved with me.
The oldest child turned 18 in January, so the child support got knocked down. They were trying to claim that she was still in school, technically she was, but she was failing and not attending. The school has finally kicked her out for lack of attendance.
Are you sure it was not just a wrong number? He would recognize his ex or daughter's voices, right? Why out of the blue call up and make a statement now if it were them? Something new other than the change in support occuring?
Fortunately for your DH the ground really won't open up and send him straight to %ell just because somebody called him up and said so.
Well anything is possible. He is as sure as he could be that it was either his ex or daughter. They block the number to try and get him to pick up, because when he sees it is them he doesn't pick up any more. As a matter of fact, he doesn't pick up blocked numbers any more either. They have not tried to contact him very much though and never by any other method than the phone.
No he is a good guy, very sad about the kids, but I have gotten to a place of acceptance - can't control other people. Even though they have done some horrible stuff, I feel kind of bad for them, as this is not the behavior of happy people. I wish I could do something to help.
It sounds to me that if it IS them, they are still hung up on everything and just trying to cause drama any way they can. Even if it is out of the blue, doesn't mean it's not just eating at them all the time.
But, if he hasn't done anything wrong then it doesn't matter what they do. The real sad thing is that they are letting it consume their lives. Misery loves company. They are unhappy so they just want to try and stir things up for you and your husband. Yeah, kind of weird and also kind of sad. (for them)
Honestly I think phone calls at 1AM are usually made under the influence. Either drugs or pills or alcohol. No one sober does that.
My SO's ex used to call under the influence and it was as bizarre as it could be, we figured out she did not know who she was calling, just random mumble or singing. So I'd think that what happened, maybe SD was partying and got drunk and mad at daddy or mom had a few.
I agree with everyone else on everything that has been said... They aren't really a part of his life and the support was lowered eventually support will stop and they can be out of your lives forever. I would change phone #s and go unlisted. Some people don't like the hassle of changing #s or being unlisted but to be completely cut off from possible harassment to me it's worth it.
Totally different but I had a # for a long time and ended up having an ex that would randomly call and text long after we were over and I was already married and moved on - I just finally changed my # to get it to stop and I haven't been bothered since -- I was harassed for years after speaking to this person and had no connection to him whatsoever just a miserable drunk a$$.. So to me it was worth it. I have a friend that is harassed the same way and she refuses to change her # bc she has had it for so many years but in the end I tell her stop
Complaining about the late night calls if you refuse to change your #... nothing towards you I'm just sharing a story.
If you have no relationship with them and your husband has decided to write them off then make it official no means for contact. Nothing says that you legally have to tell your children where you are after they are 18... Just sayin
"Honestly I think phone calls at 1AM are usually made under the influence. Either drugs or pills or alcohol. No one sober does that. " I agree.
I don't think you can "make" anyone move on and accept responsibility for their own life, unfortunately. You can just avoid them and hope they get bored with tormenting you.
Well thanks, this helps. It is sad. I wish I could do something to get them to move on. I feel like we are the big losers here, we lost the kids, no amount of money can compensate for that.
We did get an unlisted number, it was his cell phone. I turn my cell phone off every night, but he wasn't, now he is. He was afraid it was my parents who are bed-ridden - another long story. I told him to forget it, turn the phone off, there is nothing I could do for my parents at 1 am (they live an 1.5 hours away), they would have to call 911. He is now turning off the phone. A little stubborn, that man. It was stupid, but disturbing and it did disrupt my sleep.
And we are talking about re-locating when the child support case is closed. Depends on the job situation though.
Thanks for your insight.


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In the language of the Athabascan Dena'ina Indians, "Ken'ey" means "two big flats and a river cutback." This geographic description is apt for the land of the present-day town of Kenai.
...roadside shops form the businesses of many of the town's less than 400 residents. At the rest stop located near the Kenai River Bridge, travelers can view the source of the Kenai River or use spotting scopes to search nearby mountain peaks for dall...
The hiking and biking trails of the Kenai Peninsula offer access to many exquisite natural habitats, often with incredible wildlife viewing opportunities. As with any outdoor activity, remember to be prepared.
Soldotna City Council unanimously passed every action item on Wednesday's agenda. The council voted on amending municipal code, making an exception to an employment term and entering multiple construction contracts.
The hiking and biking trails of the Kenai Peninsula offer access to many exquisite natural habitats, often with incredible wildlife viewing opportunities. With any outdoor activity, remember to be prepared. Always tell someone where you are going and when you are expecting to be back, or leave written information in your vehicle at the trailhead. Carry adequate emergency supplies whenever venturing into the woods. Also, bears are everywhere and this is important to keep in mind when on the trail or in camp. Pack out whatever is packed in to keep trails they way they were found.
A trial run is underway for a 2014 Rotary Kenai River Ice Classic.
...in the Centennial riverbank restoration capital projeject fund to account for a state grant to install stairs at the Kenai River bridge. A resolution on the consent agenda would authorize the disposal of outdated city records.
Kevin Arp flips flies for red salmon in the shadow of the Kenai River bridge in Soldotna recently. The sockeye and king salmon seasons are winding down but silvers should be on the menu soon.
...trailer parked in a lot off Fireweed Avenue in Soldotna. The animals are growing in number in the neighborhood near the Kenai River bridge. Photo by M. Scott Moon Evidently, rabbit stew is not a popular dish in Soldotna. This is clear due to several...
Two Kenai City Council members have been appointed to work with the city of Soldotna and the Kenai Peninsula Borough Assembly on a plan to curb hydrocarbon pollution in the Kenai River.
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... time with exhaustion today. We haven't managed any dives since that first one as the weather has been too bad which is a massive shame but unfortunately out of our control. Instead the crew decided we should go to some Aboriginal cave on the island we were moored next to. We had to walk up the steepest rocky slope in the pouring rain which I just knew from the word go was a bad idea. Someone was going to get hurt I was sure of it. The cave was cool but only entertained us for ...
A Day of Leisure
... looking at that gorgeous view was a great pleasure. I could have stayed there all day, but I didn't want to miss the swim and Bob was waiting in the water for me. The tide had gone out so we had a very long walk out to where we could swim. I enjoyed the minnows and found a sand dollar on the way. The temperature of the water was delightful so we stayed in until a wind came up and the water became a bit rough. Back to our beach chairs and the pleasure of gazing at the ...
Back on the Bruce - Whitsundays & Bagara
... sand from a wheelbarrow and she slowly flippered her way to the sea without any of the grace we'd seen underwater. With terrible hearing and excellent sight turtles can see the difference in light on the horizon for direction. Light pollution has hatchlings spun around, often finding themselves on farms, and highways.
We returned to the centre and waited while half the group went home. We met Jason (San Francisco) who'd struggled with Australian antisemitism until ...
Hamilton Island part 1 - will we find Nemo??
... fish, pipe fish, angel fish - the list could go on and on. The captain had jokingly said in the morning that time seems to go extra quickly out on the reef and he couldn't have been more right. Before we knew it, it was time to head back on board and go home. What more can be said than a fantastic experience and one that will stay with me for as long as I live. It's devastating to think that the reef might not be there for future generations. After seeing it with my own ...
Whitehaven Beach
... over it then flew off.
Got out and sat drying for a while, watching crows raid people bags.
Boat came and collided with the beach again so went to get back. As we were walking along my flip flop broke. Was very disappointed.
Got back to the Teepee about 18:10.
Accidentally locked the teepee with the key inside. Took me about 5 minutes to undo all the clips around the outside and inside of it and get in. Seems ...


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I know Drew doesn't like the nylon available on the market, but I can't be the only one that feels like a more outdoor-friendly protective fabric would be way better. And am I right in seeing that the suit fabric has a subtle pinstripe? Not really feeling it, which is a shame since the silohuette, zips, and everything else looks top notch.
11/12/12 at 2:38pm


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The Saratoga blog
What's happening around Saratoga.
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The Marylou Whitney Rose Yankee Candle
Marylou Whitney and her husband, John Hendrickson, have given Saratoga Hospital exclusive rights to sell the new "Marylou Whitney Rose" Yankee Candle, with hopes of raising $20,000 for the hospital's new Community Health Resource Center. The special candles bear the scent of Marylou Whitney's tea rose, created in her honor in 2010 as an 85th […] [Read More]
Weekend scene in Saratoga County
With the racing season in full swing, Saratoga Springs feels like the center of the universe, or the Capital Region at least. There's plenty to do at the track, and a whole lot more around the city and surrounding area. Friday Two classic rock icons take to the SPAC stage at 7 p.m.: Santana and […] [Read More]
Yankees' Mariano Rivera to visit Saratoga Springs
Siro's Trattoria at the Lodge will welcome legendary New York Yankees pitcher Mariano Rivera for "An Evening with Mo" at 6:30 p.m. Thursday to support the Mariano River Foundation. Tickets to the exclusive event are $500 per person and will include a plated dinner and a casual conversation with Rivera. Attendees will also receive a […] [Read More]
Montel Williams in area Saturday to support local teen
Emmy-winning television personality and health advocate Montel Williams will lead the first-ever summer march in support of the The Arthritis Foundation of Northeastern NY, the Jingle Bell 5K, on Saturday at Six Flags The Great Escape and Splashwater Kingdom. Williams is walking in support of 14-year-old Michael Higgins of South Glens Falls, who has been selected as the […] [Read More]
She says 'yes' on the 19th hole
Saratoga Bridges raised over $21,000 at their 9th annual J. Michael Fitzgibbons Golf Classic on Monday at the Edison Club in Rexford. Professional golfer Briana Vega was a special guest, hitting a drive for each team, posing for pictures and signing autographs. Vega turned a bit red on the green when longtime boyfriend, David Fitzgibbons, son of J. […] [Read More]
Taste of Malta tonight
Area restaurants will be serving samples of their best fare at this year's Taste of Malta from 5:30 to 7:30 p.m. tonight. The fourth annual event is presented and sponsored by the Malta Business & Professional Association and The Adirondack Trust Company, and hosted by Hudson Valley Community College's TEC-SMART in Malta at 345 Hermes […] [Read More]
Runners/volunteers sought for Silks & Satins 5K
Nearly 1,200 runners are expected to participate in the Silks & Satins 5K Run for Special Olympics New York starting at 8 a.m. Saturday. The fast, flat course winds through the tree-lined streets of Saratoga Springs, beginning at the intersection of East Avenue and George Street. Runners can still register ahead of time online at http://tinyurl.com/2012Satins5K, […] [Read More]
Dine, dance, support backstretch workers
Good morning. There's three new events during this upcoming racing meet that will support backstretch workers at Saratoga Race Course. Ballston Spa resident Tim Iannon has organized three dinners with music and dancing in the Saratoga City Center to benefit The Race Track Chaplaincy of America, New York Division. The first event, "Dine & Dance […] [Read More]
Saratoga teen's Circus Smirkus dreams come true
Circus Smirkus' big top will once again pop up at the Saratoga Race Course Monday and Tuesday as part of the award-winning traveling youth circus' 25th anniversary tour. Among the more than two dozen youth swinging, juggling and bouncing about the big top will be 15-year-old Keenan Wright-Sanson of Saratoga Springs. A student at the […] [Read More]
TRASK art event supports restoration of Spencer Trask Memorial
Saratoga Springboard and the Saratoga Springs Preservation Foundation are teaming up for TRASK, an art show and sale to benefit the Preservation Foundation's efforts to restore the Spencer Trask Memorial and Spirit of Life sculpture in Congress Park. The benefit takes place at 6:30 p.m. Thursday at the Canfield Casino and features art work in all mediums from many local artists, […] [Read More]
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4.45 out of 10 (what is this?)
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Luke Chapman, 31 years old, lives in Elkton, MD. He has also lived in Newark, DE. He is a 2002 graduate of North East High School and 2006 graduate of University Of Delaware. He works for Jpmorgan Chase in the capacity of Bartender. Some of his favorite TV shows are Real World, Around The Horn, Csi Shows, Lonesome Dove and Law & Order Special Victims Unit. His favorite films are Finding Nemo, The Boondock Saints, Indiana Jones, Forrest Gump, The 40 Year Old Virgin and Kill Bill Movies. On the internet, Luke goes by the alias lrc07.


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Jewelry for You! How to Make Handmade Beaded Bracelets and Necklaces from Consumer Crafts and Crafts Unleashed
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How to Crochet a Bracelet
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Crochet a bracelet and have the easiest summer accessory ever! With this tutorial on How to Crochet a Bracelet from Patty Biehler, you'll be able to accessorize in no time, and you're guaranteed to have at least 1 bracelet to go with every outfit!
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I knit and crochet, plus I've been doing some beading this summer. Your e-book will give me some great ideas for Christmas gifts. Thank you soooooooooooooo much.
Where can I find the bangles online?
its a cool bracelet! I dont regularly use them, but their cute!
What a lovely easy craft. Thanks for sharing.
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Last month the car stalled after leaving car wash. Can be restarted immediately but lacked of power like no gas coming to the engine. Back to normal the next day.This time it stalled right during the car wash. Have to try a few times before it can be started.
View questions for:All Hyundai
2004 Hyundai Sonata
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2004 Hyundai Sonata Question: Stall during car wash
chowfukun, 2.7L V6, Downers Grove, IL, December 28, 2010, 11:15
1 Answer
Answer #1mastertech6371 December 28, 2010, 12:04Master
sounds like moisture is not doing well with you coils or wires.
have a shop check these to see if there is any leakage that is causing the issue.
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(6.1L V8 5-speed Automatic)
2006 Dodge Charger SRT-8 4dr Sedan (6.1L 8cyl 5A)
I wanted one of these cars and finally found the right one. It is a thrill to drive and very popular with the family. Dodge has done it ! I've had Challengers, Cudas and others and this awsome ride surpasses them all. All I can say is " Hemi baby " and I have always loved the 383 engine.
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I have had many new vehicles in the past including 1987 Buick Grand National, 1995 Impala SS, 1996 Mercedes Benz E300D, 1998 Mercedes C280 Sport, 2005 Subaru WRX STi. The SRT8 Charger I can honestly say delivers the best driving experience to date. The highway ride is outstanding, the brakes are beyond reproach and Dodge has the balls to not govern the top speed on this rocketship. (Unlike almost every other manufacturer). The fit/finish is very good and although it is a fairly heavy car the 425HP SRT8 engine makes every trip enjoyable. I highly recommend this vehicle.
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The beauty of modern engineering is that car manufacturers can now produce a vehicle such as the SRT8. A car that blends exceptional civility with very uncivilised performance. I just purchased this car a week ago. Brought it home without drama in 10 below weather, with the roads covered in ice. The tires on this monster are Goodyear F1's! Started it without a hitch in the same weather the next morning. Again, no drama. And no engine block heater either. That is not even on the option sheet for the SRT8. Not even here in Canada. Warmed the car up and drove to work. Found a patch of dry road on the way. No traffic. Dropped the hammer. Big drama! Thanks DC!
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She has all the sophistication of a classic road car with all the creature comforts a man needs. Her performance will be a ten when I add the supercharger system and all the mods to push this beast past any conventional two door sports car. Dodge hit this one right on the money with the nascar body styling and four doors for the family man. A perfect car for the midlife crisis. I love the fact everybody on the highway gets out of my way when they seen me coming from their rearview mirros. I cannot stop smiling for two hours after I go on one of my "country cruises." I have had as many thumbs up with this car as I have with my classic collection.
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I've only had my car for 5 days but it is just an incredible feeling to get behind the wheel of this car. So comfortable, solidly built, you feel safe, secure and at the same time stylish. I love everything about it!
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This is one of the finest cars I have ever purchased. It has a great ride and great feel to the road. The fuel consumption is actually pretty good considering the horsepower this car develops. I've taken it on one trip and got an average of 21.7 mpg. Please note that 98% was freeway driving at 68 to 72 mph. This car is a great value for its price.
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I thought I would never get rid of my minivan that I loved since I have 2 boys both over 6'3 and play sports but could not turn away from the Charger. Boy were they glad! Nothing has made everyone from Dad to the youngest son happier than to have a cool car to have mom drive you around in, and nothing has made me happier than to have a car I can count on. Somehow when I drive it I can get 20-21 mpg but when Dad decides to drive it goes to 14 mpg. No problem, we both enjoy it, just for different reasons. I have added 22" wheels and a pinstripe to accent the rear wheel well. Just a little to make it different. We can all take a ride it this bad boy together and no one minds at all.
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When I sold my 73 Challenger in June of 2005, I thought that was the last time I would ever feel the power of a stroked big block V8, I was wrong. This SRT8 is equal in quickness and greatly exceeds the top end speed of the 73 500" Challenger. Plus it's practical. Having a wife and two children under 4 makes this an ideal family car. To be honest, my wife drives it more than I do. She loves the power. Driving on two lane highways can be a pain if you don't have the torque to get your vehicle through the passing window of time. SRT will not let you down!
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It's hard to believe that only a few short years ago Chrysler did not even offer a V8 in a car, much less a rear drive one. They've more than made up for it. With a wife and two kids, this is a perfect choice for a family man's muscle car. Not only is it the cheapest of the SRT8's, it is also the coolest looking, with it's hood scoop and rear spoiler. The Magnum and 300C SRT8's look too much like the regular Hemi's to me.
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Since I have the Charger, people ask me "And it's fast, isn't it?" Well, it's not slow, but its 4100+ pounds take some time to get moving. 0-60 in 5 seconds according to various reviews. A sporty car, not a sports car. My 349HP MB SLK32 is a sports car. Lousy gas mileage (as expected). Nice interior. Stereo (mine has the high-end one) a bit disappointing due to some lack in bass. Seats not fully leather, but then again this is a under $40k car. Some people think the windows are too small, I don't. No idea what the stability control is there for, mine never seems to kick in. Comfortable ride. Too long to fit in my garage with the motorcycle (I traded this for a KIA Sorento which did fit).
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Community: Beloit
County: Rock
Historic Name:
Reference Number: 81881
Historic Name: Roosevelt Junior High School
Reference Number: 81882
Historic Name: Kniebusch, Charles, House
Reference Number: 83131
Date: 1909-8-25
City: Beloit
Date: 1920-11-15
Historic Name: Hilton House Hotel
Reference Number: 27628
Photographs of Public Libraries in Wisconsin
Historic Name: Dean Colley House
Reference Number: 74619
Date: 1934-1-31
Reference Number: 81883
Historic Name: Heup, H., House
Reference Number: 81442
Date: 1905 ca.
Description: Stone entrance gate to Beloit College, facing Memorial Hall.
Historic Name: Carpenter-Slater House
Reference Number: 81288
Date: 1934
Description: The members of Hangar 13, a group of Beloit youth organized to build and race model airplanes. Conrad Hansen, their organizer, is standing at left in the b...
Date: 1907-9-17
Reference Number: 81884
Reference Number: 85961
Historic Name: Loring, Ernest, House
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Date: 1920-6-19
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Description: Owney was a stray dog who wandered into the Albany, New York, post office in 1888. The clerks let him stay the night, and he fell asleep on a pile of empty mailbags. Owney was attracted to the texture or scent of the mailbags and began to follow them, first onto mail wagons and then onto mail trains. Owney began to ride with the bags on Railway Post Office (RPO) train cars across the state, and then the country. The RPO clerks adopted Owney as their unofficial mascot, marking his travels by placing medals and tags from his stops on his collar.
Creator/Photographer: Unidentified photographer
Medium: Medium unknown
Culture: American
Geography: USA
Date: 1895
Collection: Owney Collection
Repository: National Postal Museum
Accession number: A.2008-39
Persistent URL: arago.si.edu/index.asp?con=2&cmd=1&id=194391
missnae, MoreThanTheClash, and 54 other people added this photo to their favorites.
What a wonderful story, and a wonderful dog!
dog is human's best friend! I like this photo&story,and I will remember oweny.
great story :-)
Aaah what a great story :-)
Hi, I'm an admin for a group called Almost Anything BRIDGES COMP... Get entering., and we'd love to have this added to the group!
This is a wonderful story and great shot. What a great dog.
cute and great ,i love him.
I hope you enjoy reading the article I wrote about Owney. What an incredible dog!
This my be my favorite photo in the entire collection thus far. I love Owney. What a sweetheart.
GREAT dog, fabulous photo!
Owney makes a guest appearance on CuteOverload.com, featured in "Vintage ResQte!"
Must say, I quite agree... he's a cutie.
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Jimmy Smith susceptible to double moves
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Game 9: No pressure for FlaccoStory | Nov 10, 2008At the most critical times in yesterday's 41-13 rout of the Houston Texans, it became apparent Joe Flacco is no longer a rookie quarterback. He has become a clutch one. Lose your top receiver? No worries. Instead of hanging his head, Flacco looked...
Game 13: Ravens saved by Wilson's INT return in OT


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Press Release – Sign Association New President
Congratulations to our very own François Lussier for his newly appointed role as President of Sign Association of Canada for 2015. Read the full Press Release article here.


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Kayla Taylor Jazz . . . based in Atlanta, Georgia and performing Jazz, Standards & Blues from the 30s, 40s & 50s. Vocalist, Kayla Taylor & Guitarist, Steve Moore have worked together for over 16 years. Taylor & Moore made their way through the 90's in rock bands A Fine Line, OneWithout and in an acoustic duo affectionately known as The Adventures of Kayla & Steve. The team formed a jazz combo in 2001 and have developed a very loyal following. They appear in venues all over the Metro-Atlanta Area.
Currently working on their next release, "I Only Have Eyes for You", Taylor & Moore are ready for the next chapter in a never ending adventure of music & mayhem. Available on SmartyKat Records in September 2009.


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Canadian Weddings
I first met Ashley and Donovan over a cup of Tim's coffee after they've notified us that they were still looking for a wedding videographer. I think they still had to meet a couple of other videographers after our initial meeting but i guess we won them over with our charm :)Although our schedule was already maxed for the year, i got the sense that this was going to be a wonderful wedding to film. I was right.
We were literally all over Manitoba on this wedding. Jerome went to Niverville to cover the Groom, I was in Oak Bluff for the Bride prep and our trusted 3rd shooter, Rye Valderama was at Starbuck ( the town not the coffee shop ) where the ceremony was held. It was a chilly day but Ashley and Donovan and their entourage braved the elements for this wonderful wedding. Also had the chance to work again with our friend Photographer Nevin Giesbrecht who took fantastic photos. We'd also like to thank the staff at Bridges for being so accomodating and letting me film them while they were setting up.
2 x Canon 60D
Canon 5DMarkII
Sigma 70-200 2.8
Canon 135 2.0
Canon 85 1.8
Tamron 17-50 2.8 and 28-75 2.8
Vintage Vivitar Series 1 70-210 3.5
Nikkor 50 1.4
Music by Joshua Tran youtube.com/user/joshuatranmusic?feature=watch
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