text:
Page 1
TOAC Spin Labels in the Backbone of Alamethicin: EPR Studies
in Lipid Membranes
Derek Marsh,* Micha Jost,yCristina Peggion,yand Claudio Tonioloy
*Max-Planck-Institut fu ¨r biophysikalische Chemie, Abteilung Spektroskopie, Go ¨ttingen, Germany; andyDepartment of Chemistry,
University of Padova, Padova, Italy
membranes. Analogues of the Glu(OMe)7,18,19variant of alamethicin F50/5 that are rigidly spin-labeled in the peptide backbone
have been synthesized by replacing residue 1, 8, or 16 with 2,2,6,6-tetramethyl-piperidine-1-oxyl-4-amino-4-carboxyl (TOAC), a
helicogenic nitroxyl amino acid. Conventional electron paramagnetic resonance spectra are used to determine the insertion and
orientation of the TOACnalamethicins in fluid lipid bilayer membranes of dimyristoyl phosphatidylcholine. Isotropic14N-hyperfine
couplings indicate that TOAC8and TOAC16are situated in the hydrophobic core of the membrane, whereas the TOAC1label
resides closer to the membrane surface. Anisotropic hyperfine splittings show that alamethicin is highly ordered in the fluid
correspondingto atransmembrane orientation. Combination ofdatafrom the threespin-labeledpositions yieldsboththe dynamic
order parameter of the peptide backbone and the intramolecular orientations of the TOAC groups. The latter are compared with
x-ray diffraction results from alamethicin crystals. Saturation transfer electron paramagnetic resonance, which is sensitive to
,30 ns. Thus, alamethicin does not form large stable aggregates in fluid membranes, and ionic conductance must arise from
transient or voltage-induced associations.
Alamethicin is a 19-amino-acid residue hydrophobic peptide that produces voltage-dependent ion channels in
Alamethicin is a 19-amino-acid residue peptide from Trich-
oderma viride (with an N-terminal acetyl and a C-terminal
phenylalaninol) that is able to induce voltage-dependent ion
conduction across lipid membranes (1,2). The channel prop-
that consecutive conductance levels are generated by incor-
aggregate (4). The relative populations of the different con-
intrinsic curvature of the constituent lipids (5,6).
TOAC is a helicogenic nitroxyl amino acid that can be
incorporated directly in the backbone of synthetic peptides
(7–10). The nitroxide ring is rigidly attached to the Ca-atom
of the amino acid and therefore can be used as a spin-label
reporter of the orientation and dynamics of the peptide
backbone (11). Previous studies have demonstrated the util-
ity of conventional electron paramagnetic resonance (EPR)
spectroscopy to determine the location and orientation of
TOAC-labeled trichogin GA IV, a membrane-active peptide,
in lipid bilayers (12). Such methods exploit both the polarity
sensitivity (13) and the angular dependence (14) of the
nitroxide EPR spectra.
In this work, we investigate the association of TOAC-
labeled alamethicin analogs with phospholipid bilayer mem-
branes by using both conventional and saturation-transfer
(ST-) EPR spectroscopy. The TOAC residue is substituted at
one of three positions (1, 8, or 16) throughout the sequence
of alamethicin. Macroscopically aligned membranes are used
to demonstrate that the TOAC-labeled alamethicin assumes a
transmembrane orientation, consistent with the relative envi-
ronmental polarities of the different TOAC positions. Orien-
tational order parameters for the three TOAC positions allow
determination of both the angular amplitude of long-axis
motion and the intramolecular tilts of the individual nitro-
xides. Finally, ST-EPR, which is sensitive to much slower
rotational diffusion than conventional EPR (15), and the lack
of spin-spin interactions between monomers in the conven-
tional EPR, are used to obtaininformation on the aggregation
state of the peptide in the membrane.
Dimyristoyl phosphatidylcholine (DMPC) was from Avanti Polar Lipids
(Alabaster, AL). Spin-labeled derivatives of alamethicin F50/5 [TOACn,
Glu(OMe)7,18,19], with n ¼ 1, 8, and 16, were synthesized according to ref-
Submitted July 4, 2006, and accepted for publication September 14, 2006.
Address reprint requests to Dr. Derek Marsh, Tel.: 49-551-201-1285; E-mail:
Abbreviations used: Ac, acetyl; Aib, a-aminoisobutyric acid; DMPC,
1,2-dimyristoyl-sn-glycero-3-phosphocholine; EDTA, N,N,N9,N9-ethylene-
diaminetetraacetic acid; EPR, electron paramagnetic resonance; Hepes,
N-(2-hydroxyethyl)piperazine-N9-2-ethanesulphonic acid; NHtBu, tert-
butylamino; OMe, methoxy; Phol, phenylalaninol; ST-EPR, saturation
transfer EPR; TOAC, 2,2,4,4-tetramethylpiperidine-1-oxy-4-amino-4-car-
boxylic acid; V1, first-harmonic absorption EPR spectrum detected in phase
with respect to the static magnetic field modulation; V29, second-harmonic
absorption EPR spectrum detected 90? out-of-phase with respect to the
static magnetic field modulation; Z, benzyloxycarbonyl.
? 2007 by the Biophysical Society
doi: 10.1529/biophysj.106.092775
Biophysical JournalVolume 92January 2007 473–481473
Page 2
Pro-Val-Aib-Aib-Glu(OMe)-Glu(OMe)-Phol [TOAC1,Glu(OMe)7,18,19]
Pro-Val-Aib-Aib-Glu(OMe)-Glu(OMe)-Phol [TOAC8,Glu(OMe)7,18,19]
Val-TOAC-Aib-Glu(OMe)-Glu(OMe)-Phol [TOAC16,Glu(OMe)7,18,19],
where Aib is a-aminoisobutyric acid and Phol is the b-amino alcohol
L-phenylalaninol. Functional measurements demonstrate that the Gln7,18,19
to Glu(OMe)7,18,19substitution in F50/5 alamethicin does not dramatically
reduce the voltage-dependent membrane conductance that is induced by this
channel-forming peptide (18).
Sample preparation
DMPC (1 mg) and ;1 mol % of the desired TOAC spin-labeled alamethicin
(in MeOH) were codissolved in CH2Cl2, and the solution then evaporated
with dry nitrogen. After keeping under vacuum overnight, the dry mixture
was hydrated in 50 ml of 10 mM Hepes (N-(2-hydroxyethyl)piperazine-N9-2-
ethanesulphonic acid), 10 mM NaCl, 10 mM EDTA (N,N,N9,N9-ethyle
nediaminetetraacetic acid), pH 7.8 buffer, with vortex mixing at 37?C. The
lipid dispersion was then transferred to a 1 mm-diameter glass capillary and
pelleted in a benchtop centrifuge. Excess supernatant was removed and the
capillaries were flame-sealed.
Aligned planar phospholipid bilayers were formed by evaporating the
CH2Cl2solution of DMPC plus 1 mol % of TOAC-alamethicin onto the
internal faces of a quartz flat cell (Wilmad model No. WG-812, Wilmad-
LabGlass, Buena, NJ) by using a stream of dry nitrogen. Residual solvent
was removed under vacuum overnight. The oriented lipid film was hydrated
with excess buffer containing 150 mM NaCl, at room temperature. The cells
were drained and sealed immediately before measurement, with sufficient
buffer retained to ensure complete hydration throughout the experiment.
EPR spectroscopy
EPR spectra were recorded on a Varian Century-Line 9-GHz spectrometer
(Varian, Palo Alto, CA) with 100 kHz field modulation. Sample capillaries
were accommodated in standard quartz EPR tubes that contained light
silicone oil for thermal stability. Temperature was regulated by thermostated
nitrogen gas-flowthroughaquartzDewar,andwasmeasuredwithafine-wire
thermocouple situated in the silicone oil at the top of the microwave cavity.
Samples of ;5-mm height were centered in the rectangular TE102resonator,
to minimize microwave- and modulation-field inhomogeneities (19). The
microwave H1-field at the sample was measured as described in the latter
reference. Conventional EPR spectra were recorded in the in-phase first-
harmonic absorption mode (V1-display), and saturation transfer (ST-) EPR
spectra in the out-of-phase second-harmonic absorption mode (V29-display)
(20). Oriented bilayer spectra were obtained with the quartz flat cells in a
TE102rectangular microwave cavity mounted with its H1-field axis horizontal.
The entire cavity assembly was thermostated with nitrogen gas-flow.
Conventional EPR spectra were analyzed in terms of the outer and inner
hyperfine splittings, 2Amaxand 2Amin, respectively. The outer hyperfine
splitting is a useful empirical measure of the chain dynamics and ordering
that is valid in both slow and fast motional regimes of nitroxide EPR spec-
troscopy (21,22). In the motional narrowing regime, at high temperature,
Amaxisequalto theparallelelement, A//,of thepartially motionallyaveraged,
axial hyperfine tensor. The perpendicular element, A^, is derived from the
separation, 2Amin, of the inner extrema (23):
A?ðGaussÞ ¼ Amin11:3211:863log10ð1 ? SappÞ
A?ðGaussÞ ¼ Amin10:85forSapp,0:45;
where Sapp¼ ðAmax? AminÞ=½Azz?1
tensor with Cartesian elements (Axx,Ayy,Azz). The environmental polarity was
then characterized by means of the isotropic14N-hyperfine coupling, ao(13),
which is given by
2ðAxx1AyyÞ? for a spin-label hyperfine
In addition, the order parameter of the spin-label z axis, in the fast motional
regime, is given by
where the final factor on the right is a polarity correction to the hyperfine
tensor elements. Values taken for the hyperfine tensor elements are
(Axx,Ayy,Azz) ¼ (6.0 G, 7.3 G, 34.5 G) from 2,2,4,4-tetramethylpiperidine-
1-oxy in a toluene glass (24).
Saturation transfer EPR spectra were analyzed in terms of the diagnostic
line-height ratios, L$/L, C9/C, and H$/H, defined in the low-field, central,
and high-field regions of the spectra, respectively (15), and by the nor-
malized integrated intensity, IST(25). Effective rotational correlation times,
R, were obtained from ST-EPR line-height ratios, R, by using calibrations
with spin-labeled hemoglobin in solutions of known viscosity from Horva ´th
and Marsh (25). The calibrations can be expressed as (26)
R¼ k=ðR ? RoÞ ? b;
where Rois the rigid-limit value of R. The calibration constants k, b, and Ro
are given in Marsh (27). For shorter correlation times, polynomial ST-EPR
calibrations were used from Horva ´th and Marsh (28).
TOAC orientation
The crystal structure of the6T2twist-boat conformer of TOAC was taken
from molecule B of Z-TOAC-(L-Ala)2-NHtBu (29), which was obtained
from the Cambridge Crystallographic Data Centre (CCDC code: 123753). In
the available crystal structures of a-helical TOAC peptides,6T2is by far the
most prevalent conformer of TOAC (30). The crystal structure of native
alamethicin (31) was obtained from the Research Collaboration for Struc-
tural Bioinformatics protein database (32) (PDB code: 1amt). The TOAC resi-
due was substituted for the Aib residue at position 1, 8, or 16 in alamethicin
by constraining the transformed coordinates of the TOAC N, Caand C9 atoms
to coincide with those in alamethicin, by using nonlinear least-squares
The orientation uzof the nitroxide z axis of TOAC to the alamethicin
molecular axis was determined as described in Marsh (30). The molecular
axis of alamethicin was taken as the axis of the longer (N-terminal) helical
section. The latter was defined as the line equidistant from the Caatoms of
residues 4–14, for which the mean radial distance is 2.38 A˚, by nonlinear
least-squares fitting. The vector connecting the Caatoms of residues 1 and
19 was also used as an alternative definition of the molecular axis. The un-
published structure of [TOAC16, Glu(OMe)7,18,19]-alamethicin (33), and
variants in which the TOAC residue from position 16 was substituted for the
Aib1or Aib8residue, were used in an analogous manner to obtain the
orientation of the TOAC nitroxyl axes. This alamethicin analog has the6T2
twist-boat conformer of TOAC that is found in the Z-TOAC-(L-Ala)2-
NHtBu reference peptide.
Conventional spin-label EPR spectra
Fig. 1 shows the EPR spectra of the three different TOAC1,
474Marsh et al.
Biophysical Journal 92(2) 473–481
Page 3
in DMPC bilayer membranes. Spectra are shown at various
temperatures, both above and below the lipid chain-melting
temperature of 23?C. In the gel phase, the spectra are in-
dicative of strong immobilization on the nanosecond time-
scale, but that of the TOAC1derivative also evidences strong
spin-spin broadening. The latter is seen most clearly at 10?C
as a strong distortion of the baseline by a very broad un-
derlying component (dotted-line spectrum). This spin-spin
interaction is caused by aggregation of the spin-labeled
alamethicin in the gel-phase membrane. The sharp features
in the low-temperature spectra most probably arise from a
population of noninteracting spin labels, and therefore in-
dicate some heterogeneity in the degree of aggregation. The
aggregation observed by spin-spin interaction in the gel-
phase correlates well with functional studies on the effects of
the lipid chain-melting transition. The transmembrane cur-
rent density mediated by alamethicin in unsupported bilayers
of 1-stearoyl-3-myristoylphosphatidylcholine was found to
increase dramatically on entering the gel phase of the mem-
brane from the fluid phase (34). The current density in the gel
phase at 24?C corresponded to a pore concentration of ;106
pores/cm2and decreased to a low level representing only
1 pore/cm2in the fluid phase. Increased pore density implies
an increased degree of peptide aggregation and an increased
local concentration of alamethicin in the gel phase that
manifests itself here as an increase in spin-spin interactions.
Spin-spin broadening is absent from the EPR spectra in
the fluid phase at 1 mol % spin label. This finding indicates
that the spin-labeled alamethicin is randomly dispersed in
the lipid membrane at temperatures above the lipid chain-
melting transition. Similar conclusions have been reached
from EPR studies on alamethicin with a flexible spin label
at the N- or C-terminus (35,36). The spectra from the three
TOAC derivatives are still highly anisotropic, indicating high
ordering or limited motion, in the fluid phase. The extent
of spectral anisotropy decreases with increasing temperature.
At higher temperatures, the spectra display axial motional
averaging, as indicated by the well-defined outer and inner
hyperfine splittings, 2Amaxand 2Amin, respectively (see, e.g.,
Temperature dependence
Fig. 2 shows the temperature dependence of the outer hyper-
fine splitting, 2Amax, for alamethicin with the three different
positions of TOAC labeling in DMPC membranes. For the
TOAC8and TOAC16derivatives, there is a small but abrupt
decrease in the value of Amaxat the DMPC chain-melting
transition. This decrease in Amaxcorresponds to an increase
in rotational dynamics of alamethicin on lipid chain fluid-
ization. The apparent increase in Amaxfor the TOAC1deriv-
ative at 23?C most probably is an artifact arising from the
spin-spin broadening of this particular label in the gel phase.
The values of Amaxat temperatures immediately above the
lipid transition are still indicative of a high degree of order, or
limited amplitude of angular motion, of the spin-labeled
alamethicin. There are, nonetheless, differences between the
values of Amaxand their rate of change with temperature for
the three different label positions. For each label, the values
of Amaxdecrease steadily in response to the increased extent
of lipid chain motion with increasing temperature.
Isotropic hyperfine couplings
Fig. 3 gives the temperature dependence of the effective
isotropic hyperfine couplings, ao, defined by Eq. 3, for the
different positions of TOAC labeling. The true value of ao
should depend only on the polarity of the environment in
which the spin label is situated (see, e.g., (38,39)), and not on
the molecular motion. Therefore, it is expected to be ap-
proximately independent of temperature. Artifactual in-
creases in effective values with decreasing temperature are
found at lower temperatures, due to the breakdown of
motional narrowing theory (on which Eqs. 3 and 4 are based)
caused by slow-motional contributions to the spectra at the
lower temperatures. This provides a useful experimental cri-
terion by which to define the temperature region over which
motional narrowing theory is valid. Fig. 3 shows that the
values of aoare practically constant for the TOAC1and
TOAC8analogs at temperatures of 55?C or higher. Only
for the TOAC16analog is the temperature dependence
tra (V1-display) of [Glu(OMe)7,18,19]
alamethicin analogs with TOAC substi-
tuted for (A) residue 1, TOAC1; (B)
residue 8, TOAC8; and (C) residue 16,
TOAC16, in DMPC bilayers at the tem-
peratures indicated. (Solid lines, total
scan width ¼ 100 Gauss; dotted lines,
total scan width: 160 Gauss.)
Conventional EPR spec-
TOAC-Alamethicin in Membranes 475
Biophysical Journal 92(2) 473–481
Page 4
fine couplings are: ao¼ 15.41 6 0.01 G, 15.01 6 0.01 G,
and 15.03 6 0.07 G, for the TOAC1, TOAC8, and TOAC16
analogs, respectively. The lower values indicate that the
TOAC8and TOAC16analogs are situated considerably
deeper in the hydrophobic core of the membrane than is the
TOAC1analog. For the TOAC16analog, only the values at
higher temperature were taken and are associated with a
higheruncertainty,asindicated.Fora TOACpeptide inwater,
the isotropic hyperfine coupling is considerably higher: ao¼
16.2–16.5 G, depending on the pH/charge state of the peptide
(40). This implies that the TOAC1analog is not directly
exposed to water, but is probably located close to the polar-
apolar interface of the membrane, as suggested also for
surface-associated trichogin GA IV and melanocortin (41,42).
Note that a higher water exposure of TOAC1than of TOAC16
(at high temperature) does not exclude the possibility of a
yet greater water exposure of a spin label attached to the
C-terminal phenylalaninol, especially at lower temperatures
Orientational order parameters
Fig. 4 gives the temperature dependence of the order param-
eters, Szz, for the three TOAC analogs of alamethicin in
DMPC bilayer membranes in the fluid phase. According to
the criterion of constant ao, the data in Fig. 3 suggest that
motional narrowing theory should be applicable above 55?C
for all except the TOAC16analog. As already noted, the
spectra are axially symmetric in this temperature regime (see
Fig. 1). The values given in Fig. 4 therefore should be rea-
sonably representative of the time-average angular amplitude
of the spin-label z axis, relative to the director for the uniaxial
rotational motion. At temperatures below those for which data
is given in Fig. 4, motional narrowing theory can no longer be
relied upon for determining order parameters.
Fig. 5 shows the conventional EPR spectra of the three
TOAC-containing analogs of alamethicin in aligned multi-
bilayers of fully hydrated DMPC in the fluid phase. Spectra
are shown for the static magnetic field parallel (solid lines)
and perpendicular (dashedlines) tothe normalto the orienting
quartz substrate on which the multibilayers are deposited.
Although the degree of alignment of the sample is probably
not completely homogeneous, and at these temperatures (30–
32?C) the rotational motion is not yet in the fast regime
(especially for the TOAC16analog), there is clear anisotropy
between spectra recorded with the parallel and perpendicular
orientations of the magnetic field. For all three TOAC posi-
tions, the largest hyperfine splitting of the first derivative-like
stant, Amax, for [Glu(OMe)7,18,19] alamethicin TOAC1(squares), TOAC8
(circles), and TOAC16(triangles) analogs in DMPC bilayers.
Temperature dependence of the outer hyperfine splitting con-
couplings, ao, for [Glu(OMe)7,18,19] alamethicin TOAC1(squares), TOAC8
(circles), and TOAC16(triangles) analogs in DMPC bilayers.
Temperature dependence of the effective isotropic hyperfine
Szz, for [Glu(OMe)7,18,19] alamethicin TOAC1(squares), TOAC8(circles),
and TOAC16(triangles) analogs in DMPC bilayers. Solid lines are experi-
mental measurements; dotted lines are a nonlinear least-squares fit of Eq. 6
to the temperature dependence, with constant uzfor each TOAC position
(see text and Fig. 6).
Temperature dependence of the effective order parameters,
476Marsh et al.
Biophysical Journal 92(2) 473–481
Page 5
single absorption lines is obtained in the parallel orientation
with the magnetic field lying along the substrate normal (see,
e.g., (14)). This finding confirms that the director, N, for the
uniaxial rotation lies along the membrane normal, consistent
with a transmembrane orientation of the alamethicin molecule
as indicated by the isotropic hyperfine couplings.
For uniaxial motional averaging, the EPR order parameter
of TOAC is given by the addition theorem for spherical
Szz¼ ÆP2ðcosgÞæ3P2ðcosuzÞ;
where g is the angle that the principal rotational diffusion
axis, R, of the TOAC-labeled alamethicin molecule makes
with the membrane normal, N, and uzis the inclination of the
spin-label z axis to R (see Fig. 6). P2(x) ¼ (3x2?1)/2 is a
second-order Legendre polynomial and the angular brackets
indicate a time average over the rotational motion. Because
the TOAC spin label is rigidly attached to the peptide back-
bone, the different values of Szzin Fig. 4 imply different
orientations, uz, of the three TOAC residues to R. The crystal
structure of native alamethicin reveals an a-helical conforma-
tion thatis bent (31). This bend, togetherwith localdistortions,
could account for the dependence of the TOAC order param-
eters on residue position.
The dotted lines in Fig. 4 represent a nonlinear least-
squares fit of Eq. 6 to the temperature dependence of Szz
for all three TOAC labels, under the assumption that the
spin-label inclination to the diffusion axis is temperature-
independent. The order parameter of the diffusion axis, rela-
tive to N, then varies from ÆP2(cosg)æ ¼ 0.87–0.70 over the
temperature range 60–85?C. The local orientation of the indi-
vidual spin labels is characterized by the fixed values uz¼
30?, 25?, and 20? for TOAC1, TOAC8, and TOAC16, re-
spectively. Judging from the goodness of the fits in Fig. 4,
only for TOAC1are there significant changes in uzwith
temperature, possibly corresponding to a local unwinding of
the helix or other conformational reorientation at the first
residue position.
TOAC orientation in alamethicin
Fig. 7 shows one of the molecules (A) in the crystal structure
of native alamethicin (31) into which the crystal structure of
the TOAC moiety from molecule B of Z-TOAC-(L-Ala)2-
NHtBu (29) has been incorporated at residue position 1, 8, or
16. This was done by constraining the coordinates of the
alamethicin analogs in aligned DMPC bilayers. (Solid lines, magnetic field
parallel to the membrane normal; dashed lines, magnetic field perpendicular
to the membrane normal.) (A) TOAC1-alamethicin at 30?C; (B) TOAC8-
alamethicin at 32?C; and (C) TOAC16-alamethicin at 32?C. Total scan
width ¼ 100 Gauss.
Conventional V1-EPR spectra of [TOACn, Glu(OMe)7,18,19]
brane. The principal molecular diffusion axis, R, is inclined at instantaneous
angle g to the membrane normal N. The nitroxide z axis is oriented at con-
stant angle uzto R. The experimental order parameter, Szz, of the nitroxide
z axis is given by Eq. 6, where, for axial symmetry, ÆP2(cosg)æ is the order
parameter of the alamethicin diffusion axis.
Orientation of TOAC-labeled alamethicin in a lipid mem-
TOAC-Alamethicin in Membranes477
Biophysical Journal 92(2) 473–481
Page 6
TOAC N, Ca, and C9 atoms to coincide with those of Aib1,
Aib8, or Aib16in alamethicin. If the axis of the longer helical
segment is defined as the line that is equidistant from the Ca
atoms of residues 4–14, the inclination of the nitroxide z axis
to this axis is ua¼ 7?, 15?, and 34?, for TOAC at residue
positions 1, 8, and 16, respectively. For the recently solved
structure of [TOAC16, Glu(OMe)7,18,19] alamethicin (33),
the orientation of the spin-label z axis to the longer helical
axis is ua¼ 10–12? and, for this TOAC structure grafted
at residue positions 1 and 8, is ua¼ 4–7? and 8–9?, re-
spectively. In terms of residue position, these values for the
TOAC orientation uaare in the opposite order to those of uz
that are derived from the EPR results. From this, one must
conclude that the diffusion axis does not coincide with the
helical axis between residues 4 and 14, as defined above.
Taking the more recent crystal structure, the rotation axis R
is tilted relative to the principal helix axis by ;30?. This
value may be somewhat of an upper estimate because of the
effects of local helix distortions that were referred to above.
Note that taking the mirror-image2T6twist-boat conformer
of TOAC would predict nitroxide z-axis orientations that are
incompatible with the EPR order-parameter measurements
Saturation transfer spin-label EPR spectra
Fig. 8 shows the saturation transfer EPR spectra of the
three different TOAC1, TOAC8, and TOAC16analogs of
[Glu(OMe)7,18,19] alamethicin in DMPC bilayer membranes.
Spectra are shown at various temperatures, both above and
below the lipid chain-melting temperature of 23?C. The
spectra are scaled to line height, rather than to the absolute
intensity, which decreases with increasing temperature. In
the gel phase, the ST-EPR spectra have appreciable intensity,
not only in the overall spectrum, but also in the diagnostic
regions at intermediate positions in the low-, high-, and
center-field manifolds of the14N-hyperfine structure. These
nonvanishing ST-EPR intensities reflect the response of the
peptide to the extremely slow rotational diffusion of the
gel-phase lipids, with effective correlation times beyond the
microsecond regime (45,46). Immediately above the lipid
chain-melting transition, the overall intensity of the ST-EPR
spectrum drops abruptly and the spectral lineshape changes
because of preferential reduction in the line heights at the
diagnostic L99, C9, and H99 positions relative to the stationary
turning points at positions L, C and H, respectively (see, e.g.,
(15)). These spectra are characteristic of very fast motion, no
longer in the microsecond regime (at least for the central C9
region of the spectrum), and reflect the response of the
peptide mobility to the rapid lipid chain motions in the fluid
membrane phase (45,47).
Fig. 9 gives the temperature dependence of the normalized
ST-intensity, IST, and the diagnostic line-height ratios, L99/L,
C9/C, and H99/H, for the TOAC8alamethicin analog in
DMPC membranes. This is the analog with highest ST-EPR
intensities and, therefore, that most likely to exhibit any
microsecond motions that are associated with the whole
peptide. All four ST-EPR parameters clearly reflect the
change in overall peptide dynamics at the gel-fluid phase
transition, which occurs at ;23?C. The values of the C9/C
line-height ratio and of the ST integral are very low in the
fluid phase, beyond those for which ST-EPR calibrations
were made. This corresponds to an effective rotational
correlation time of ,2.9 3 10?8s (28). In addition, the L99/L
ratio is seen to increase with increasing temperature, i.e.,
with decreasing correlation time. This is a feature of incipient
motional narrowing in ST-EPR spectra (15), that again is
consistent with correlation times of ,10?7s. Such rapid
rotational reorientation suggests rather strongly that the
peptide is not aggregated in fluid DMPC membranes, as
was concluded already from the lack of spin-spin broadening
of the conventional EPR spectra. Standard hydrodynamic
theory (see, e.g., (48)) predicts a rotational correlation time
of 1.4–2.8 3 10?7s for a single transmembrane a-helix in a
membrane of effective viscosity 2.5–5 P (49). For a dimer,
this value increases to 3.5–7.0 3 10?7s, and for a tetramer to
5.6–11.3 3 10?7s. It therefore seems most likely that the
TOAC alamethicin analogs are monomeric (at a concentration
ecule A of native alamethicin ((31);
PDB: 1amt) with the TOAC structure
from molecule B of Z-TOAC-(L-Ala)2-
NHtBu ((29) CCDC: 123753) substi-
tuted for Aib at residue position 1, 8, or
16. The alamethicin molecule is ori-
ented relative to the membrane surfaces
as predicted in the OPM database (53).
Crystal structure for mol-
478Marsh et al.
Biophysical Journal 92(2) 473–481
Page 7
of 1 mol %) in fluid DMPC bilayers, as suggested previously
foralamethicin in vesicles ofunsaturatedphosphatidylcholines
(35,36). Note that substitution of Gln residues, particularly
the conserved Gln7, by Glu(OMe) might reduce somewhat the
propensity of alamethicin to form pores, as suggested by the
lower conductance (18).
ties of the environments of the different TOAC positions
demonstrate that Glu(OMe)7,18,19alamethicin adopts a trans-
membrane orientation in fluid bilayer membranes of DMPC.
This is in agreement with other spectroscopic studies on un-
modified and chemically labeled alamethicins (43,50). Cer-
tain models for the induction of ion channels propose a
switching of the alamethicin long-axis from a surface to a
transmembrane orientation (see, e.g., (2)). The present
results show that the bulk of the peptide has a transmembrane
orientation and therefore such channels are most likely
formed by self-assembly within the membrane.
The combined order parameter measurements from the
different TOAC positions indicate that the tilt of the long
axis of the peptide, relative to the membrane normal, is fairly
small with values of ÆP2(cosg)æ corresponding to effective
tilt angles of 17–27? over the temperature range 60–85?C. It
is expected that the tilt of alamethicin is restricted because
the length of the molecule (;29 A˚from Caof residue 1 to
Caof residue 19) is relatively short compared with the
thickness of a DMPC bilayer. For the latter, the hydrophobic
thickness is ;26 A˚and the total thickness is ;37 A˚at 30?C,
which extrapolate to 21 A˚and 30 A˚, respectively, at 85?C
using an expansion coefficient of ?0.004 per degree (51).
Orientation of alamethicin according to the distribution of
polarity/hydrophobicity in the molecule, as reported in the
OPM database (see Fig. 7), predicts a transmembrane align-
ment of alamethicin with a hydrophobic depth of 28 A˚and a
tilt of 16 6 8? (52,53). This theoretical predictionis therefore
essentially in accord with the present experimental measure-
An interesting feature of the angular motion of the TOAC
spin labels, relative to that of spin-labeled lipid chains (see,
e.g., (54)), is that the rotational diffusion is slow on the EPR
timescale (;ns) in fluid membranes, except at rather high
[Glu(OMe)7,18,19] alamethicin analogs with TOAC substituted for (A)
residue 1, TOAC1; (B) residue 8, TOAC8; and (C) residue 16, TOAC16, in
DMPC bilayers at the temperatures indicated. Total scan width ¼ 160
Saturation transferEPRspectra(V29-display) of
circles), and diagnostic line-height ratios, L99/L (squares), C9/C (solid
circles), and H99/H (triangles), from the ST-EPR spectra of [TOAC8,
Glu(OMe)7,18,19] alamethicin in DMPC bilayers.
Temperature dependence of the integrated intensity, IST(open
TOAC-Alamethicin in Membranes479
Biophysical Journal 92(2) 473–481
Page 8
temperatures (.60?C). This reflects the rigidity of the helical
backbone of alamethicin and the anchoring of the TOAC
ring at the Ca-position of the helix.
On the longer (ms) timescale of ST-EPR, however, rotation
about the long axis of alamethicin is relatively rapid. This
means that alamethicin is not forming large pore aggregates,
which would have rotational correlation times in the micro-
second regime. Most likely, the bulk of the alamethicin is
monomeric in the fluid membrane, and pore formation (and
growth) occurs via transient association of the monomeric
species. This is in accordance with the electrophysiological
channel behavior and proposals from other spectroscopic
studies (43).
We thank Frau B. Angerstein and Frau B. Freyberg for skillful technical
assistance, Dr. Marco Crisma for providing the coordinates of [TOAC16,
Glu(OMe)7,18,19] alamethicin, and Frau I. Dreger for help with preparing
Fig. 7.
1. Nagaraj, R., and P. Balaram. 1981. Alamethicin, a transmembrane
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2. Sansom, M. S. 1991. The biophysics of peptide models of ion chan-
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lipopeptaibol in membranes using TOAC as an ESR probe. Biopolymers.
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spin label ESR spectra. Chem. Phys. Lipids. 82:7–14.
TOAC-Alamethicin in Membranes481
Biophysical Journal 92(2) 473–481


text:
Legal Question in Criminal Law in Kansas
pulled over for cracked windshield, the ticket is for 1998 mazda and i dwas driving a 1996 mazda is this fightable?
Asked on 9/27/13, 6:47 am
1 Answer from Attorneys
Anthony Smith LawSmith
0 users found helpful
0 attorneys agreed
Read more
You could raise this as a defense. But, often a Prosecutor will amend the citation. If you get it replaced, the Prosecutor might agree to dismiss or amend to a non moving violation.
Good luck
Answered on 9/28/13, 5:11 am
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subset: commoncrawl


text:
N.Y.C. Store Pickup Ready Within the Hour
A pair of updates to existing camcorders put Panasonic's AG-AC130A and AG-AC160A on a slightly higher plane than their predecessors. This month Panasonic is expected to make available these two improved models.
At the NAB 2012 show, Panasonic announced the AG-HPX255, an update to its popular AG-HPX250 handheld camcorder. The AG-HPX255 carries over all the existing advanced features of its predecessor and adds a big one: Compatibility with the Panasonic AG-EC4G extension control unit.
JVC announced two powerful ENG cameras at NAB 2012, the GY-HM600 and GY-HM650. Both of these professional handheld HD camcorders feature 1/3-inch 1920 x 1080 12-bit CMOS sensors, F11 light sensitivity at 2000 lux, a fast f/1.6 Fujinon Auto Focus 23x zoom lens, and more.
Advancements in technology have allowed manufacturers to shrink the size of broadcast-quality camcorders, while at the same time squeezing in better performance. A case in point is Sony's new PMW-100 camcorder; ENG professionals sporting this compact new unit will appreciate the lighter load and greater maneuverability.
Sony has announced its new NEX-FS700U Super35 camcorder, an update to the popular NEX-FS100 interchangeable-lens professional model. This is no mere refresh, however; the FS700 has greatly expanded capabilities over the earlier model.
It took a while for the whole world to catch up to the HDTV movement, but nowadays you have to go out of your way even to find a TV that's not HD. A similar situation exists with camcorders, although there are still plenty of SD camcorders for sale. Even so, JVC has just announced the GY-HMQ10, a handheld 4K camcorder that you'll be able to buy in March.
People see in 3D because they have two eyes, each relaying a slightly different perspective to the brain, which then combines the two images to create a sense of depth. Traditional cameras and camcorders can never capture 3D because they have only one lens.
It's very easy today to record stunning HD video, even with an inexpensive camcorder. But if you want to achieve the video quality demanded by professionals you should be using a large-sensor camcorder. Here's a look at some of the highest functioning large sensor camcorders on the market.
Consumers have more choices in camcorders than ever before, and some of them seem downright professional with features sets that are packed to the rafters. Canon's VIXIA HF G10 Flash Memory Camcorder shoots 1920 x 1080 high definition video, and you can choose frame rates of 60i, 30p or 24p for a cinematic look and feel.
Anyone can take a basic camcorder, capture an important event or family gathering and put together a reasonable, if somewhat amateurish presentation that friends and family can enjoy. But when you watch a segment on the evening news, you expect the footage to look professional.
Some professional camcorders can cost an arm and a leg. Sony's PMW-F3 falls into that category. Fortunately, manufacturers realize that not everyone can afford to part with an arm and a leg, so they find ways to offer products with similar feature sets to the pro cameras, but which can be had for just an arm.
Sony's new NEX-FS100UK (B&H kit) will give videographers plenty to think about when it comes to choosing their next video or still camera. Is it better to shoot video with a DSLR that takes stunning stills? Or would it be worth upgrading to a DSLR video camera that can capture beautiful stills as well?
When it comes to ENG video capture, form factor and performance are paramount, and with a total weight of 8.2 lb (including the lens, 4.3" LCD screen, microphone and battery) and phenomenal image quality, the JVC GY-HM750U ProHD solid state compact shoulder camcorder makes for an attractive video recording package.
Professional videographers expect a certain level of quality and an advanced feature set in the equipment they use. In other words, professional videographers rely on professional equipment to produce professional-quality work. Unfortunately, such equipment is usually larger and heavier than they would like. Canon has addressed this problem with its new and compact XA10 professional camcorder.
Sony has been delighting video professionals with their XDCAMs since 2004.They introduced their first high-speed memory XDCAM EX in 2007, and went on to set high standards in broadcast quality with their PDW-700 and PMW-320 models.
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Prices, specifications, and images are subject to change without notice. Not responsible for typographical or illustrative errors. Manufacturer rebates, terms, conditions, and expiration dates are subject to manufacturers printed forms NYC DCA Lic.: Elec. Store #0906712 Elec. & Home App. Serv. Deal. #0907905; Sec. Hd. Deal. Gen. #0907906


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text:
Tags » Voices In Your Head
5 Things to Know About the Voices in Your Head
I was talking to a friend yesterday who was discussing how exhausting "the committee" inside her head was. Also known as: the running commentary, the voices in my head, and "am I crazy?" 663 more words
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text: Egypt court overturns government decree on civilian arrests Egypt Egypt Priyanka Boghani on Dec 31, 1969 @ 7:33 PM An Egyptian protester scuffles with security forces as they arrest a fellow demonstrator outside the Saudi embassy in Cairo. Egypt's administrative court on June 26, 2012, ruled against a government decree that would have allowed the military to arrest civilians. Egypt's administrative court on Tuesday suspended a justice ministry decree that allowed the military to arrest civilians, according to Agence France Presse. After reviewing an appeal filed by 17 rights groups, the court decided against the controversial decree that was passed on June 13, said AFP. The government's decision would have allowed military police and intelligence to arrest civilians, and caused outrage among activists and politicians, who compared it to imposing martial law, according to the Associated Press. The groups appealing the decree said it created "extraordinary powers that have no basis in law" and represented "a blatant circumvention of the official end of the state of emergency," according to AFP. More on GlobalPost: Mohammed Morsi, Muslim Brotherhood candidate, named president of Egypt "The court has blocked the decision of the Minister of Justice that gave military and military intelligence officers powers of arrest," said Cairo administrative court Judge Ali Fikry, according to Reuters. The decree would have given military personnel a right that only police officers had previously, said Ahram Online. More on GlobalPost: Ahmed Shafiq leaves Egypt after losing election The crimes which the decree would have applied to included "crimes and misdemeanors harmful to the government," "possession/use of explosives," "resisting orders issued by those in power or assaulting them," "destruction of public property or historic monuments," "strike-actions at institutions that serve the public interest or assaulting the right to work," and "intimidation and thuggery," said Ahram Online. Egypt has installed Islamist Mohammed Morsi as its new president after months of unrest under the ruling military. More on GlobalPost: Banking on Africa's poor


text:
(9) DePaul 66
(21-3, 8-1 Big East)
(2) Connecticut 89
(22-1, 10-0 Big East)
2:00 PM ET, February 5, 2011
STORRS, Conn. -- Geno Auriemma looked down at the stat sheet and was amazed.The second-ranked Huskies shot 68 percent from the field and held DePaul (No. 11 ESPN/USA Today, No. 9 AP) to just 41 percent shooting in an 89-66 rout on Saturday."What you don't realize during the game is what your shooting percentage is," Auriemma said. "You don't see that during the game and don't appreciate it till afterwards. I thought we played pretty well today -- especially the second half."Maya Moore had 34 points, 11 rebounds and six assists while making 14 of her 16 shots."Maya, you've seen her do that a lot," Auriemma said. "Maya's got the ability that once she gets it going, it just piles up with her."The Huskies won for the 100th time in their last 101 games, the lone loss coming to Stanford on Dec. 30. Junior guard Tiffany Hayes has been a part of all 100 victories, making her the fastest player in NCAA history to reach the century mark.Stefanie Dolson added a career-high 21 points for the Huskies (22-1, 10-0 Big East), who have won 74 straight at home and 60 consecutive games in the conference."That was by far her best game at Connecticut," Auriemma said. "The way she moved and things she did."The victory left UConn in sole possession of first place in the conference. The Huskies head down to West Virginia on Tuesday and host Notre Dame, which has one Big East loss, on Feb. 19."This year we might have to go undefeated to win the regular season," Moore said. "There's a lot of talented teams. We needed to play well everytime it's a Big East game. We don't want to have to leave it to someone else losing."Felicia Chester scored 19 points while Keisha Hampton and Anna Martin added 14 each to lead DePaul (21-3, 8-1), which had won eight straight and reached its best ranking ever this week.The Blue Demons, who were 8-0 in the Big East for the first time, had already knocked off Stanford and West Virginia this season. The school set up a viewing party at their on-campus arena for fans who wanted to watch this game, which was DePaul's biggest ever in the conference.DePaul gave its fans back in Chicago a lot to cheer about early on. Hampton's layup gave the Blue Demons a 19-16 lead midway through the first half.UConn answered quickly, though, scoring the next 11 points, including six by Moore. Bria Hartley's layup made it 27-19 with 8:11 left in the half, and after the Blue Demons cut their deficit to six on a hook shot by Chester, UConn and Dolson responded again.The Huskies closed the half with 11-5 burst, capped by Dolson's three-point play with 20 seconds left. UConn led 45-33 at the half, shooting a sizzling 70 percent from the field."We've gotten better defensively, but 70 percent in the first half and 68 in the game means we didn't defend them very well," DePaul coach Doug Bruno said.Dolson had 15 points by the break -- one off the freshman center's previous career high set against South Florida.Moore, who is 48 points short of the career Big East scoring record set by Louisville's Angel McCoughtry, ended any hopes of a DePaul come back. After another Chester hook shot to start the second half, Moore scored six points during an 11-0 run that put the game away.Her touch pass set up Dolson's jumper and made it 54-35 with 16:49 left in the half, and DePaul couldn't get within 14 the rest of the game.UConn, which leads the nation in assists, finished the game with 27 -- including a career-high nine by Kelly Faris."We are an unselfish team and we like to share the ball and feed each other," Moore said.Moore as well as Auriemma and Bruno were given rings before the game by U.S. women's basketball director Carol Callan for helping the national team win the gold medal at the World Championship last October. The three were also presented with the trophy from that tournament that will go on display at the school.The Blue Demons are staying in the area before traveling to face Syracuse on Tuesday. DePaul will practice in Springfield on Sunday at the Naismith Basketball Hall of Fame and then tour it."After they give themselves 10 minutes of reflection on what they can do better, I want them to remember that they are putting together a special season," Bruno said.
Copyright by STATS LLC and The Associated Press
Team Stat Comparison
|FG Made-Attempted
|26-63 (.413)
|36-53 (.679)
|3P Made-Attempted
|6-18 (.333)
|6-11 (.545)
|FT Made-Attempted
|8-12 (.667)
|11-19 (.579)
|Fouls (Tech/Flagrant)
|17 (0/0)
|9 (0/0)
|Largest Lead
Game Leaders
Next 5 Games
|02/08 @SYR 7:00pm
02/12 MARQ 8:00pm
02/15 RUTG 9:00pm
02/20 GTWN 5:30pm
02/23 @LOU 7:00pm
|02/08 @WVU 7:00pm
02/12 @PROV 2:00pm
02/14 OKLA 7:00pm
02/19 ND 2:00pm
02/22 HALL 7:00pm
|Complete Schedule: DePaul | Connecticut


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stupid newbie question...
VeryVee(toronto)May 18, 2005
I thought i saw this in a FAQ somewhere but i can't find it...
Oh well maybe i just imagined it to be a frequent question.
Anyway here goes ..
What is the difference between a viola, violette and violet ?
Just wanted to know...
etii(France 8)
Easy to answer :-)
Take care.
Hello !
Easy to answer :-)
Viola (general latin name) is the family. In that family there are 2 members: pansy and violet.
Violette is just the French for violet :-)
The problem: all names started with viola so that U CAN'T make the difference between pansy and violet thanks the name :-(
Once when I was a totally newbie I bought a viola Rebeca and discover it was a pansy (YUKY !!!)
Take care.


text:
Aids And Hiv Treatment Counseling
Here's a taste of what TheBody.com has to offer on this topic:
Get the Facts
Women, Depression and HIV/AIDS...and HIV care, treatment, and disease progression. For example, HIV-positive women with chronic (long-lasting) depression go to their medical visits less regularly and are more likely to progress quickly to AIDS. Starting HIV treatment and adhering to HIV medications (taking your HIV drugs correctly) are less likely in chronically-depressed HIV-positive women. If you are experiencing symptoms of depression, you may miss doses, take the...
Ask the Experts
HIV+ without treatmenthi,Dr.Josephmy Brother has been HIV+ since last year, unfortunately he still couldnt face the truth, he has psychological problems now and also refuses to receive any treatment..so I would like to ask u how long a HIV+ patient will live without any...
Response from Joseph McGowan, M.D., F.A.C.P.
North Shore University Hospital...experienced medical provider who can work with him to accept his diagnosis and explore easy to take treatments. He may also need some counseling and therapy to address his "psychological problems" at the same time.Best of luck,Joe Read more »
HIV/AIDS counseling/counseling referrals?What is the 800 National Hotline for HIV/AIDS counseling/counseling referrals for straight as well as gay individuals? The one listed in my Internet search was for the CDC, and they do not provide this counseling inforamtion. Thanks.
Response from Ewald Horwath, M.D.
University of Miami School of Medicine...The CDC National AIDS Hotline (1-800-342-AIDS) does not provide counseling, but it does provide information on where to go for HIV testing and counseling. The counseling is designed to explain the test Read more »
hiv counselinghow to counsel the pre and after testing hiv patients?
Response from Joseph McGowan, M.D., F.A.C.P.
North Shore University Hospital...HIV testing is best accompanied by counseling to provide education about HIV and how it is transmitted. The testing process in the US is undergoing "streamlining" in order Read more »
Group Therapy and Counseling ResourcesDear Michael, I am writing as a graduate student in Counseling and an HIV Positive gay man. I'm interested in any/all resources in counseling (individually), marital/family therapy models and group psychotherapy with gay men with HIV Disease. I've...
Response from Michael Shernoff, M.S.W.
Psychotherapist and Author...College/London PressShernoff, M (1999) AIDS and Mental Health Practice: Clinical and Policy Issues Haworth PressShernoff & Smith (2000)HIV Treatment: Mental Health Aspects of Antiviral Therapy, San Francisco, UCSF AIDS Health ProjectI strongly... Read more »
The Role of HIV Testing, Counseling and Treatment in Coping With HIV/AIDS in Uganda: A Qualitative Analysis... The current qualitative study explores the role of HIV voluntary counseling and testing (VCT) and treatment in helping HIV-positive Ugandans cope with the disease. The researchers conducted 12 predetermined Read more »
Nigerian Advocacy Group Encourages HIV-Positive Couples to Marry, Offers Counseling, Other Services...spreading to HIV-negative people, the AP/Google.com reports. The program is run by the Bauchi Action Committee on AIDS and offers counseling and money to couples who are living with HIV/AIDS and are planning to marry. According to the... Read more »
Prevalence of Sexually Transmitted Infection/HIV Counseling Services Received by Teen Males, 1995-2002...set out to determine whether improvements have been made in the delivery of sexually transmitted infection (STI) and/or HIV counseling services to teenage males. Analysis was performed on data from two nationally representative surveys of 15-... Read more »
Zambia's HIV/AIDS Council Director Urges People to Be Tested on National Voluntary Counseling, Testing Day...traditional leaders, civil society, the private sector and communities to "mobilize" people to access HIV services and to scale up HIV treatment and prevention programs. He added that HIV prevention programs should include a multifaceted,... Read more »
Connect With Others
Need advice-get hubby to counseling
Posted by wifey289, 5 Replies...even though it can cause liver damage since he's on inh. i'm trying to be a supporter and not a mother here but i know he NEEDS counseling. can any of u manly men or wives w/ one tell me how i can get him to counseling? orders do not take well.... Read more »
Couples counseling?
Posted by Carrie...I'm just curious if anyone knows of counseling places that help with serodiscordant couples. I live in Minnesota and have contacted the Minnesota AIDS Project and they don't Read more »
What is HIV?
Posted by Anonymous...or local Ministry of Health through their Web site or telephone services to determine where to find local HIV screening and counseling services. What do I do if I test HIV-positive? Do not panic. Most health practices, clinics, and centers have... Read more »
please help
Posted by Anonymous, 2 Repliesplease answer my questions. I have two questions. The first is how long a person will stay with HIV before he turns to Aids without having treatments. And how long will he stay with HIV if he had treatment before turning to AIds. I have tested... Read more »
Resource Centers
Guides for People With HIV


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Peony 'Mrs. Franklin D. Roosevelt'--Where to find?
marya13(6A)August 25, 2010
Does anyone know where I could order the peony "Mrs. Franklin D. Roosevelt"? I have a new garden, and this is my favorite peony--although it took me a while to find the name; I remembered it as "Eleanor Roosevelt"!
Thank you!


text:
YOU ARE HERE: Stocks » News & Commentary »
'Deezer, Adami launch music competition'
Deezer France has launched its sixth annual music competition in partnership with recording artists' association Adami. Acts have until 31 January to sign up. Three winners will be picked by three separate juries, the public, professionals and VIPs on 1 June. Each winner will receive EUR 10,000 in help from Adami, including a Deezer Session and a concert at the Casino de Paris venue.
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Making yet one more effort to keep Raphael Musto from reporting to a federal medical facility Monday, attorneys for the former state senator have filed an appeal with the U.S. Third Circuit court of Appeals arguing the trip to the North Carolina facility alone could kill Musto.
On Jan. 7, Musto was found mentally incompetent to stand trial, and U.S. District Judge A. Richard Caputo then ordered him into the custody of the Attorney General, to be committed to the Federal Medical Facility in Butner, N.C. Musto's attorneys asked Caputo to stay that order until they could file an appeal, but Caputo rejected the motion.
Caputo noted putting Musto in custody of the Attorney General was a legally-mandated result of being declared incompetent, and called the request to stay the order "incongruous."
This afternoon Musto's attorneys filed a 99 page "emergency motion" with accompanying memorandum and exhibits, asking the appeals court to issue an emergency, 21 day stay of Caputo's Jan. 7 order, so the government could issue a response and Musto could reply.
"For the reasons set forth at length in the forthcoming memorandum, even the trip to North Carolina may prove fatal to Appellant," Musto's attorneys wrote.


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Archive: HuachucaShowing 100-200 of 1641 items, Subscribe to RSS Feed
Childhood obesity: a preventable disease
Oct 7, 2014
National Disability Employment Awareness Month is October
Oct 6, 2014
October is National Disability Employment Awareness Month. The purpose of this designation is to raise awareness about disability employment issues and celebrate the many contributions of America's workers with disabilities.
Enhancing resiliency: Forces Command Soldiers intervene, help Army "battle buddies"
Sep 29, 2014
U.S. Army Forces Command units across the United States are helping ensure suicide prevention and awareness among their Soldiers, Forces Command G-1 personnel officials say.
Army, Air Force move data over same network for first time at Joint Base San Antonio
Sep 24, 2014
This month, the Army and Air Force achieved major network security and capacity upgrades at Joint Base San Antonio, Texas, in partnership with the Defense Information Systems Agency. This is the first Department of Defense location to achieve the pairing of new switching technologies and security stacks. In addition, network speed for end users has increased dramatically.
Leadership Huntsville/Madison County gains better understanding of Arsenal
Sep 19, 2014
When counting the dollars and cents in Huntsville/Madison County, the Redstone Arsenal story is a crucial part of the equation. Leadership Huntsville/Madison County class members paid a visit to Redstone Arsenal Sept. 17 to hear that story as part of their Economic Base Day, which gave participants a deeper understanding of the economic drivers in the Tennessee Valley. The group's visit on post included an economic overview of Team Redstone, bus tour of Redstone Gateway and the Arsenal itself, and a missile firing at Redstone Test Center.
New NCOER expected to more accurately assess Soldiers' performance
Sep 18, 2014
On Aug. 1, 2014, the secretary of the Army approved the new Non-Commissioned Officer Evaluation Report. Implementation will be in September 2015.
Soldier missing from Korean War accounted for
Sep 18, 2014
The Department of Defense POW/Missing Personnel Office announced today that the remains of a U.S. serviceman, missing from the Korean War, have been identified and will be returned to his family for burial with full military honors.
Geospatial analysis tool built to support Army intelligence analysis
Sep 17, 2014
The ability to obtain this information, analyze it and produce a report for commanders has been enhanced by the Situational Awareness Geospatially Enabled geospatial tool, supporting the Distributed Common Ground System-Army.
Brown: Soldiers should be trained like elite athletes
Sep 15, 2014
Soldiers and elite athletes share traits like discipline, talent, teamwork and commitment and deserve the same type of scientific-based training, said Lt. Gen. Robert B. Brown, commander, Combined Arms Center and Fort Leavenworth, Kansas.
Two Vietnam War Soldiers receive Medal of Honor
Sep 15, 2014
Retired Command Sgt. Maj. Bennie G. Adkins and Spc. 4 Donald P. Sloat were awarded Medals of Honor for valor in Vietnam, during a ceremony at the White House, Sept. 15, 2014.
Traffic changes at Fort's East Gate begin Monday due to major construction
Sep 12, 2014
Fort Huachuca, Arizona - The east gate on Fort Huachuca receives a major facelift beginning Monday. The project will impact traffic flow into the installation and there will be delays.
Young artist helps local PD catch criminals through her work
Sep 12, 2014
Fort huachuca, Arizona - Makenzie Sargent, 18, was one of two students whose ideas were blended together to create a colorful new logo and poster the police department is using as part of their Crime Solvers program. The posters will be displayed in area buildings to show that the business actively works with the SVPD to identify and arrest criminals. The logo appears on the Crime Solvers website.
Steelhead Triathlon
Sep 12, 2014
Fort Huachuca, Arizona - "We had a total of 150 participants, including team members," said Leslie Woods, chief of Sports, Fitness and Aquatics. Woods founded the triathlon, consisting of swimming, biking and running, in 1994, and has continued organizing the event every year since.
Logistics Readiness Center provides different facets of support on post
Sep 12, 2014
Fort Arizona, Arizona - Formerly known as the Fort Huachuca Directorate of Logistics, the LRC is part of the 404th Army Field Support Brigade, located at Joint Base Lewis-McChord, Washington. On Fort Huachuca, the 230 LRC person-workforce, a mix of Department of the Army Civilians and contractors, serve 17,127 Soldiers and Civilians.
Assessing Army Safety
Sep 12, 2014
As the new director of Army Safety and commanding general of the U.S. Army Combat Readiness/Safety Center, I look forward to working with Army leaders and safety professionals at all levels to preserve our Army's readiness through the prevention of accidental loss of our Soldiers, civilians, Family members and vital resources. First, however, I'd like to recognize some of those whose efforts have been so instrumental in turning the accidental loss arrow downward over the past few years.
Patriot Day and National Day of Service and Remembrance 11 September 2014
Sep 11, 2014
"Like the great wall and bedrock that embrace us today, nothing can ever break us. Nothing can change who we are as Americans."
Talking lethality with Army's senior non-commissioned officer
Sep 10, 2014
Sgt. Maj. of the Army Raymond F. Chandler III discussed the future of lethality with Army Technology Magazine.
Future Fires: Integrating technology solutions
Sep 9, 2014
Army leaders are looking to the future force and seeking to be revolutionary in their thinking about integrating technology, according to current guidance from Army Chief of Staff Gen. Raymond Odierno. Army researchers are exploring technology solutions to enable improved lethality and fires.
Technologies to sustain the Army of 2025 and beyond
Sep 8, 2014
New sustainment technologies will help the Army retain overmatch with a more capable, leaner expeditionary force.
Investing in the Army's future
Sep 5, 2014
The U.S. Army vision for lethality science and technology investment is to enable overmatch in weapon systems for both offensive and defensive capabilities.
High Tech Tools Save Lives Faster, Cheaper, Smarter
Sep 5, 2014
U.S. Southern Command's Science, Technology and Experimentation division wowed the crowd at the Conference Center of the Americas on Wednesday, showcasing a wide spectrum of technologies that could help in disaster relief and humanitarian operations. The cutting-edge gadgetry included drones, portable water purification equipment, satellite communications that fits in luggage, and a device that breaks through language barriers with ease.
Women's Equality Day: FH celebrates at Alvarado Hall
Sep 5, 2014
Fort Huachuca, arizona - "Celebrating Women's Right to Vote" is this year's theme, commemorating the 1920 passage of the 19th Amendment to the Constitution, granting women the equal right to vote.
Force protection exercise proves successful at Fort Huachuca
Sep 5, 2014
Fort Huachuca, Arizona - On Aug. 27 at approximately 8 a.m., an active shooter in the 309th Military Intelligence Battalion "shot" and "killed" a noncommissioned officer during a morning formation in Prosser Village, on Fort Huachuca.
Feisty female among strong fighters in 2014 All Army Boxing Trials
Sep 5, 2014
Fort Huachuca, Arizona - Feisty female among strong fighters in 2014 All Army Boxing Trials
Special Operations is part of Army Intelligence Heritage
Sep 5, 2014
Fort Huachuca, Arizona - Special Operations is part of Army Intelligence Heritage
Army Suicide Awareness Month 2014 tri-signed letter
Sep 4, 2014
Army Suicide Awareness Month 2014 tri-signed letter
'Start Strong:' Every career in the Army starts with TRADOC
Sep 2, 2014
Through U.S. Army Recruiting Command, the U.S. Army Center for Initial Military Training and U.S. Army Cadet Command, U.S. Army Training and Doctrine Command serves as the foundation for the "Start Strong" phase of every Soldier's career.
Message from the Commanding General United States Army Intelligence Center of Excellence
Aug 28, 2014
Sesame Street Muppets visit Fort Huachuca, ease stress of military relocation
Aug 28, 2014
Sesame Street Muppets visit Fort Huachuca, ease stress of military relocation
Major construction including lane closures, increased traffic to start at East Gate
Aug 28, 2014
Fort Huachuca, Arizona - Everything from traffic pattern changes to removal of the old guard shack are about to take place. Phase one of the Hatfield Paving Project starts Sept. 15 and is tentatively scheduled to be completed by Nov. 20.
Don't miss All Army Boxing
Aug 28, 2014
Fort Huachuca, Arizona - All Army Boxing
Suicide Prevention Training -- "I've got more important things to do!"
Aug 28, 2014
Fort Huachuca, Arizona - "I've got more important things to do!" I've heard this statement while waiting to give a suicide prevention class. To tell the truth, before I started working in Suicide Prevention, at times I felt this way when I was ordered to attend suicide prevention training. I wasn't thinking about what I could get out of the training. I was feeling sorry for myself because I had to sit through another session of what is sarcastically known as GAT, or Good Army Training.
Alsos Mission seizes French government arsenal, September 1944
Aug 28, 2014
Fort Huachuca, Arizona - Alsos Mission seizes French government arsenal
Sgt. Maj. of the Army Raymond Chandler III visits Fort Huachuca
Aug 21, 2014
Sgt. Maj. of the Army Raymond Chandler III speaks to Fort Huachuca, Ariz. Soldiers about battle buddy safety during a town hall meeting held at Cochise Theater, Aug. 20.
Sergeant Major of the Army talks Fort Huachuca's leadership, success in Army's future
Aug 21, 2014
Fort Huachuca, Arizona - As force reduction kicks into gear, Soldiers can't help but begin to question what the future of the Army will hold -- and those assigned to Fort Huachuca are no different.
Unmanned Aircraft Displayed For Inspector General
Aug 18, 2014
REDSTONE ARSENAL, Ala. -- All unmanned aircraft systems were a go -- although simulated -- for Lt. Gen. Peter Vangjel when he visited the Joint Integration Systems Laboratory during his time at Redstone Arsenal on Aug. 5.
Fort holds memorial ceremony for recently retired FH police chief
Aug 15, 2014
Fort Huachuca, Arizizona - Soldiers, Civilians and Family members gathered for a memorial ceremony Aug. 8 at the Main Post Chapel on Fort Huachuca for Ollie James Shears, who passed away June 8.
Steelhead Triathlon turns 20, Event organizer reminisces -- his background led to creation of FH eve
Aug 15, 2014
Fort Huachuca, Arizona - As participants prepare for the 20th Annual Steelhead Triathlon here at Fort Huachuca, Leslie Woods, chief of Sports, Fitness and Aquatics -- the mastermind of all things sports and fitness at Barnes Field House -- sits in his office and reminisces over the past two decades of the triathlon event.
Flooding still underway this year -- avoid water runoff problems
Aug 15, 2014
TRADOC accreditation team to visit USAICoE
Aug 15, 2014
Fort Huachuca, Arizona - Fort Huachuca's Quality Assurance Office, or QAO, has the mission to prepare the United States Army Intelligence Center of Excellence, USAICoE, for the Training and Doctrine Command's accreditation visit. A failed accreditation can affect the ability to train Soldiers and negatively impact the military training institution.
Army civilians maintain key mobile satellite terminal
Aug 14, 2014
Dan Vadella, an electronic integrated systems mechanic at Tobyhanna Army Depot, checks for loose wires on the rubidium tray of a Secure Mobile Anti-Jam Reliable Tactical Terminal (SMART-T).
66th MI Soldiers muck, ruck through Tough Mudder
Aug 12, 2014
Soldiers from 66th Military Intelligence Brigade, 24th MI Battalion, A and B Companies took part in the Rhein-Main Tough Mudder in Hirzenhain, Germany August 10.
New Army PT uniforms result of Soldier feedback
Aug 11, 2014
A new Army Physical Fitness Uniform will become available service-wide, beginning in October next year.
FH Army Substance Abuse Program encourages mental health readiness
Aug 11, 2014
Fort Huachuca, Arizona - Being ready -- whether for a mission, a job, or family responsibilities, requires a person to be mentally and physically healthy.
Be prepared: Tuition Assistance changes coming soon
Aug 11, 2014
Fort Huachuca, Arizona - Over the past few months important changes have been taking place for those who receive or are hoping to receive tuition assistance, or TA, from the Army.
TRADOC names NCO, Soldier of the Year
Aug 11, 2014
After a week of challenging competition, the command responsible for training the Army chose its best Soldiers during the 2014 Noncommissioned Officer and Soldier of the Year ceremony here, Aug. 8.
Enterprise Challenge 2014 highlights intelligence on-the-move, technology demonstrations
Aug 8, 2014
Fort Huachuca, Arizona - The Army's participation in Enterprise Challenge 2014 highlighted increased on-the-move intelligence capabilities using the Tactical Intelligence Ground Station, an element of the Distributed Common Ground System -- Army, or DCGS-A.
Fort Huachuca Soldier pins first Army Instructor Badge
Aug 8, 2014
Fort Huachuca, Arizona - The Army began awarding Army Instructor Badges this summer to bolster the standing of the NCO instructors who teach the courses in the Noncommissioned Officer Education System, according to Liston Bailey, chief of the Institute for Noncommissioned Officer Professional Development Learning Innovations and Initiatives Division, in the May 6 edition of the NCO Journal.
Seven Army retirees honored in Installation Retirement Ceremony
Aug 8, 2014
Fort Huachuca, Arizona - Seven Army retirees honored in Installation Retirement Ceremony
Skin cancer: education, prevention and protection
Aug 8, 2014
Through education, prevention and protection, you can reduce the risk of developing skin cancer.
Main Post Chapel celebrates 239th Chaplain Corps Anniversary
Aug 8, 2014
Fort Huachuca, Arizona - The Main Post Chapel hosted the 239th Chaplain Corps Anniversary Aug. 1 with a ceremony in the sanctuary, a tour through the building and lunch in the activity room. Around 70 Soldiers, Civilians and Family members attended the fellowship event to learn more about the history and roles of Army chaplains and chaplain assistants.
Army researcher fighting Ebola on front lines
Aug 6, 2014
Army researchers are working on developing vaccines for the deadly Ebola virus, as well as combating the spread of the virus and caring for those who are infected. The virus has recently killed more than 800 in Sierra Leone, Guinea and Liberia.
Army general killed in Afghanistan
Aug 6, 2014
An American general was killed Aug. 5, 2014, in Kabul, Afghanistan, by an individual wearing a uniform of the Afghan National Security Forces.
Combat Lifesaver Course trains Soldiers to save lives on, off battlefield
Jul 31, 2014
Fort Huachuca, Arizona - In the event of an emergency, Army combat medics play a critical role by applying first aid and trauma care to wounded Soldiers, but in some situations the first person to assist isn't always a certified medic.
Online tool provides 'voice' for installation customers
Jul 31, 2014
Fort Huachuca, Arizona - When it comes to providing feedback on services provided by various organizations throughout the Department of Defense, the Interactive Customer Evaluation, or ICE, system allows customers to voice their feedback.
Families earn free admission to Blue Star Museums
Jul 31, 2014
Fort Huachuca, Arizona - Families have until Sept. 1 to explore more than 2,000 Blue Star Museums for free this year. The program is a collaboration among the National Endowment for the Arts, Blue Star Families, Department of Defense and participating museums.
Deployable Cadre Program seeking more volunteers
Jul 31, 2014
As the Army Contracting Command's expeditionary mission increases, the ACC Deputy Chief of Staff, Human Capital G-1, says the need for deployable civilians is increasing.
Family Life Chaplains, Part I: The creation of the Family Life program
Jul 30, 2014
Exploring the history and impact of the U.S. Army Chaplain Corps' Family Life program within our Army over a two part series.
Army investigators warn public about romance scams
Jul 30, 2014
Special Agents from the U.S. Army Criminal Investigation Command, commonly known as CID, are once again warning internet users worldwide about cyber criminals involved in an online crime that CID has dubbed "the Romance Scam."
Protect yourself from new-to-U.S. virus carried by mosquitoes
Jul 29, 2014
A virus called "chikungunya" carried by mosquitoes that are common in the continental United States is likely to appear in locations where Soldiers, Army civilians and their families work and live.
NETCOM commanding general to pin on second star
Jul 25, 2014
Brig. Gen. John B. Morrison Jr., NETCOM commanding general, will be promoted to major general.
CECOM Office of Small Business Holding Outreach Event
Jul 24, 2014
"The U.S. Army Communications-Electronics Command's Office of Small Business Programs will hold a small business outreach event, Tuesday, July 29th, at Building 6008 (Mallette Training Facility) on Aberdeen Proving Ground.
U.S. Army, General Motors and Raytheon announcing new training initiative for transitioning Soldiers
Jul 24, 2014
Tuesday, July 29, Deputy Chief of Staff G-1 Lt. Gen. Howard B. Bromberg joined by Lynn A. Dugle, Raytheon Company Vice President, and Steve Hill, General Motors Vice President, will kick-off the "Shifting Gears" Automotive Technician Training Program during a proclamation signing beginning at 9:30 a.m. in the Pentagon Courtyard.
Public Health Command tracks new mosquito-borne disease
Jul 23, 2014
The U.S. Army Public Health Command is responding rapidly to an emerging viral infection that is threatening the United States.
CECOM Commanding General promoted to major general
Jul 22, 2014
Brigadier General Bruce T. Crawford, Commanding General, U.S. Army Communications-Electronics Command and APG Senior Mission Commander, was promoted to major general during a promotion ceremony July 21 at the Myer Auditorium here.
Army researchers develop Cargo Pocket ISR
Jul 21, 2014
Researchers at the U.S. Army Natick Soldier Research, Development and Engineering Center in Massachusetts, are developing a pocket-sized aerial surveillance device for Soldiers and small units operating in challenging ground environments.
Chow from a 3-D printer? Natick researchers are working on it
Jul 18, 2014
Army researchers are investigating ways to incorporate 3-D printing technology into producing food for Soldiers.
Eyes in the Sky
Jul 15, 2014
The Unmanned Aerial Vehicle, or drone, is considered to be one of the most technically advanced assets the Army has at its disposal and has been used to support combat, security and stability operations throughout Afghanistan. From 2001 to 2008 alone the amount of UAVs in the military increased 25-fold, putting the total UAV assets at more than 5,000. With such a large increase in the use of UAVs, the importance of Sgt. 1st Class Lee Priest's job cannot be overemphasized.
Researchers improve smokes, obscurants technology
Jul 11, 2014
ABERDEEN PROVING GROUND, Md. (July 11, 2014) -- During a recent event to study the performance of smoke and obscurants, researchers from the U.S. Army Research Laboratory's Survivability/Lethality Analysis Directorate set out to improve the process of measuring transmissivity.
311th SC(T) welcomes Curry as command's new CSM
Jul 11, 2014
The 311th Signal Command (Theater) held a change of responsibility ceremony, Tuesday, with Command Sgt. Major Darris Curry accepting the symbolic Noncommissioned Officer's Sword from Command Sgt. Maj. Allen Braswell.
Army tests putting NERO electronic warfare jammer on UAVs
Jul 10, 2014
Last month within the mountainous terrain of The Great Salt Lake Desert at Dugway Proving Ground, Utah, the U.S. Army conducted flight testing of an unmanned airborne electronic attack capability known as NERO, or the Networked Electronic Warfare Remotely Operated.
Colonel Charles Young: Buffalo Soldier and Intelligence Officer
Jul 9, 2014
Fort huachuca, Arizona - More than 10,000 black men served in the regiments honorably called the Buffalo Soldiers. Some of these men, such as Henry O. Flipper and Benjamin O. Davis, are historically prominent and fairly well-known to students of American history. However, a great number remain unknown or their accomplishments buried as footnotes to history. One such man with a significant link to Fort Huachuca and military intelligence is Colonel Charles D. Young.
Reinvigorating the Army's deployment readiness
Jul 8, 2014
The Army Power Projection Program Rapid Expeditionary Deployment Initiative will improve expeditionary deployment readiness and help the Army provide forces to meet global combatant commander requirements on short notice.
Scientists break new ground with 3-D printing composites
Jul 7, 2014
ABERDEEN PROVING GROUND, Md. (July 7, 2014) -- When Army research and development investments in additive manufacturing pay off, future warriors who need hard-to-get devices, such as unmanned aerial vehicles or medical devices, may be able to print them on the spot.
LTG Alva R. Fitch Creates MI Branch
Jul 7, 2014
Fort Huachuca, Arizona - Alva R. Fitch graduated from the US Military Academy in 1930. The United States was in the midst of the Great Depression, still recovering from the loss of life and innocence that came from their participation in World War I. At the tender age of 23, the young 2nd Lieutenant couldn't have imagined that another war on a global scale was brewing overseas.
New STEM program launches on APG
Jul 7, 2014
"Someday you are going to make a difference." A group of Harford County high school students heard these encouraging words during the opening ceremony of a new Army STEM program at the APG Recreation Center ballroom Monday.
Independence Day 2014 tri-signed letter
Jul 3, 2014
Celebrating the 200th Anniversary of the Star-Spangled Banner and the Soldiers, families and civilians who uphold its Honor and Dignity
111 MI Bde. changes leaders during ceremony with unusual touches
Jul 3, 2014
Fort Huachuca, Arizona - The 111th Military Intelligence Brigade began its change of command ceremony on Chaffee Parade Field with two special events to bid farewell to its outgoing Commander Col. Richard "Mike" Monnard, and to welcome incoming Commander Col. Kevin Wilkinson, June 27.
'Number one Intel captain' receives Weinstein Award
Jul 3, 2014
Fort Huachuca, Arizona - As part of the Military Intelligence Corps Hall of Fame Induction two-day event, Maj. Gen. Robert Ashley, commanding general, U.S. Army Intelligence Center of Excellence and Fort Huachuca, along with members of the Weinstein Family, took a moment to recognize a Soldier who excels in the Military Intelligence field. They presented him with the Lt. Gen. Sidney T. Weinstein Award for Excellence in Military Intelligence, June 27.
Mission completed for FH MI soldier, Olympic Medal Received
Jul 3, 2014
Fort Huachuca, Arizona - Representing both the U.S. Army and the United States, Capt. Chris Fogt, an officer currently attending the Military Intelligence Captain's Career Course here, won a Bronze medal during the 2014 Olympic Winter Games in Sochi, Russia.
New HT-JCOE award recognizes Army honor graduates
Jul 3, 2014
Fort Huachuca, Arizona - The Human Intelligence Training -- Joint Center of Excellence has begun recognizing the Army honor graduates of its Source Operations Course and the Defense Advanced Tradecraft Course with the Col. KB Reid Human Intelligence Award.
1st Signal Brigade welcomes new CSM at change of responsibility ceremony
Jul 2, 2014
The 1st Signal Brigade held its Change of Responsibility Ceremony at USAG Yongsan's Knight Field June 25.
Under Secretary Carson poses 10 questions to Army
Jul 1, 2014
Under Secretary of the Army Brad Carson posed 10 questions to members of the Association of the U.S. Army at their monthly breakfast, June 26, 2014, dealing with the size and composition of the Army, modernization and readiness.
CECOM hosts global communications exercise at Aberdeen Proving Ground
Jul 1, 2014
"Effective Cyber operations in a Joint Information Environment" was the focus of this year's Joint User Interoperability Communications Exercise (JUICE) hosted by U.S. Army Communications-Electronics Command at Aberdeen Proving Ground, Md., during the month of June.
309th MI Bn. gains new leader
Jun 30, 2014
Fort Huachuca, Arizona - In a well-attended ceremony by both Soldiers and Civilians, Lt. Col. Laura Knapp, 309th Military Intelligence Battalion outgoing commander, passed the guidon to Lt. Col. Erich Spragg, incoming commander, June 20 on Fort Huachuca's Chaffee Parade Field.
OCS Hall of Fame inducts USAICoE chief of staff
Jun 30, 2014
Fort Huachuca, Arizona - Col. Jeffrey Jennings, chief of staff of the U.S. Army Intelligence Center of Excellence, Fort Huachuca, and an Arizona native, was inducted into the U.S. Army Officer Candidate School, or OCS, Hall of Fame during a ceremony May 19, in the National Infantry Museum at Fort Benning, Georgia.
NETCOM welcomes new senior enlisted leader
Jun 30, 2014
Fort Huachuca, Arizona - The U.S. Army Network Enterprise Technology Command, NETCOM, held an assumption of responsibility ceremony for the command sergeant major position June 20, in the command's auditorium in Greely Hall.
Family Child Care Program here offers unique employment opportunity
Jun 30, 2014
Fort Huachuca, Arizona - As Suzette Gray's family started to grow, her Family time was decreasing.
CECOM CG and Senior Mission Commander of APG Nominated and Confirmed for Second Star
Jun 30, 2014
Brig. Gen. (P) Bruce T. Crawford, commanding general of U.S. Communications-Electronics Command was confirmed for promotion to major general on June 25 by the U.S. Senate.
Army invites public to comment on environmental impacts of possible force reductions
Jun 26, 2014
The Department of the Army has completed a Supplemental Programmatic Environmental Assessment (SPEA) for Army 2020 force structure realignment and is making a draft Finding of No Significant Impact (FNSI) available for public comment. All interested members of the public, federally-recognized Indian or Alaska Native tribes, Native Hawaiian groups, federal, state, and local agencies are invited to review and provide comments. Comments will be accepted until August 25.
Aberdeen Proving Ground Celebrates 154th Anniversary of the Signal Corps
Jun 24, 2014
Brig. Gen. Bruce T. Crawford, commanding general of the U.S. Army Communications-Electronics Command, took the opportunity during a celebration of the Signal Corps' 154th anniversary to recognize the regiment's past contributions while addressing its relevancy for the future strategic environment.
41st Signal Battalion welcomes new commander
Jun 23, 2014
The 41st Signal Battalion hosted a change of command ceremony June 16 to mark the change of Battalion leadership of the "First Brigade's Best" at the soccer field across from Burke Towers.
20th CBRNE Command hones capabilities during exercise
Jun 20, 2014
The U.S. Army's sole formation dedicated to combating the full range of Chemical, Biological, Radiological, Nuclear and Explosive threats facing our nation is honing its mission command capabilities during a nation-wide training exercise this week, in Maryland, Texas, and California.
Change of Command starts anew with one, says farewell to another
Jun 20, 2014
Fort Huachuca, Arizona - Lt. Col. Jaren Price, outgoing commander, passed the guidon to Col. Richard Monnard, brigade commander, 111th MI Brigade., who then passed it to Lt. Col. Candice Frost, incoming commander, during the event, representing a symbolic transfer of leadership.
309th MI Bn. welcomes new command sergeant major
Jun 20, 2014
Fort Huachuca, Arizona - Holding onto their belongings, Soldiers, Families and members from surrounding communities welcomed Command Sgt. Maj. Gregory Roman as the newest member of the 309th MI Bn. team and bid farewell to outgoing Command Sgt. Maj. Julie Guerra.
NETCOM G5 retires, next step -- teaching math
Jun 20, 2014
Fort Huachuca, Arizona - A combined retirement and retreat ceremony was held in front of the U.S. Army Network Enterprise Technology Command headquarters, Greely Hall, June 10, as the Army said farewell to a Soldier with 30 years of service to the Army and the nation.
William Harryman, presents Male Survivors of Sexual Assault to Fort Huachuca Unit Victims
Jun 20, 2014
Fort Huachuca, Arizona - William Harryman, MSC, NCC, MS presents Male Survivors of Sexual Assault to Fort Huachuca Unit Victim Advocates.
NETCOM Soldiers vie for top spots
Jun 19, 2014
Soldiers from Signal commands and brigades from around the globe pushed themselves and each other through grueling events here June 9-13, during the 2014 Network Enterprise Technology Command Noncommissioned Officer and Soldier of the Year Competition.
Special Forces - A Smaller Footprint
Jun 18, 2014
Experts at a conference on special operations forces in Tampa make the case for special forces operators, globally networked across nations as a cost-effective solution to threats, like terrorism, extremism and hostile neighbors. Features soundbites from Vice-Admiral Sean Pybus, Commander, NATO Special Forces Headquarters and Stu Bradin, CEO Global SOF Foundation and footage from a multi-national live exercise in Tampa, Florida. Also available in high definition.
APG JUICE Active Shooter Simulation Training Event
Jun 18, 2014
The Aberdeen Proving Ground Police Department together with Team Command, Control Communications, Computers, Intelligence, Surveillance and Reconnaissance (C4ISR) conducted an active shooter training simulation in conjunction with the Joint Users Interoperability Training Exercise (JUICE) on June 10, 2014 at APG.


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Written on: 26/04/2008
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