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AIU Adds American Football To Schedule Southampton Owner Provides $30M Loan Combined Debt Of EPL Clubs At $3.7B RFU CEO Vows To Keep Six Nations Format EPL May End Title Sponsorship Jevans' Resignation Rocks ER2015 Prep Budweiser To Launch Dream Goals Spot Event Notes Hangin' With ... Richard Wright Berlin, Glasgow To Host New Event In '18
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Demand For NBA In Europe On The Rise As Pistons Face Knicks In London
Published January 17, 2013
GROWING GLOBAL DEMAND: In Detroit, Vince Ellis noted Morel said that "there is demand for the NBA overseas." He cited "the popularity of basketball during last summer's London Olympics when Team USA won the Gold." Players like Lakers G Kobe Bryant and Heat F LeBron James are "internationally recognized, and with the growing popularity worldwide, there is a thought that Europe is a natural fit for the league." Morel said of Thursday's game, "Because there's no game during the regular season physically happening on the ground ... it's just something that gets the authenticity and real experience to our fans." Ellis noted Morel "promised a lively atmosphere," but the question is "what happens after Thursday?" Ellis: "Can the NBA grow to be more than an entertainment novelty in Europe?" (DETROIT FREE PRESS, 1/15). In N.Y., Marc Berman reported Knicks F Steve Novak said, "We understand the future of the NBA and future of basketball. It's hard not to think one day the NBA won't expand overseas. Obviously, there's a ton of NBA fans in London and pretty much everywhere in Europe.'' The Knicks "arrived at the team hotel in downtown London without fanfare." No one was "staked out seeking autographs" (N.Y. POST, 1/16).


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DAA Entertainment Presents The Dreaming – is a reunion of Stabbing Westward's founding members. The songwriting team is back with singer Christopher Hall and keyboardist Walter Flakus, along with the one-time Stabbing Westward drummer Johnny Haro. The band is rounded out with Deadsy / Orgy guitarist Carlton Bost and bassist Franccesca D'Struct...Save
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lorieuDecember 14, 2007
i have an enlarged lymph node at the base of my leg, near my panty line. it has been there for probably about a year by now, and it is about 1.5 cm. I am pregnant, so the doctors cant give me antibiotics, and due to the length of time its been there, they wanted to check it out immediately. they did a FNA(Fine Needle Analysis)yesterday(it hurt like hell!!!)Does anybody have an idea of how worried i should be?


text:
By DIANE KETCHAM
Published: December 29, 1996
Memorial Mired in Dispute
IT was born out of death -- the death of two young men who had everything to live for. A memorial has arisen at the site of a crash that killed Eric Lowry 19, a graduate of Sayville High School, and Jason (Hags) Haggerty, 18, a senior at the school. But unlike other roadside memorials, this shrine has taken on a life of its own.
Every day, it seems, something new is added to the plethora of bouquets, Christmas decorations, shirts, crosses, candles and a large sign left on the south side of Montauk Highway in Oakdale. With the additions has come a controversy. Is the memorial that was erected because of one accident going to cause another?
''We've gotten calls about it,'' said Richard LaValle, the chief engineer of the Suffolk County Department of Public Works. ''People are concerned that drivers are slowing down to read the sign and that may cause an accident.''
The county issued a permit to Al Sirico, the owner of the Sunset Sign company of Centereach, to erect a 6 by 8 foot sign at the site. It says: ''To All Long Island Young People: Don't end up a statistic. Don't mix alcohol and autos. Take care of each other in honor of our lives. Don't drink and drive. We are watching you in heaven, love Eric and Jason.''
The driver of the car, another senior at Sayville High, William Balkin, 18, survived the crash and has been charged with driving while intoxicated.. Mr. Sirico said a Sayville resident called him and asked if he would create a sign about the dangers of mixing drinking and driving. ''I've done things like this before,'' the sign-maker said. ''It's a way of giving to the community.''
But the sign on busy Montauk Highway, a county road, is causing traffic problems. ''If we're advised that it's a safety hazard, we will have to do something,'' Mr. LaValle said. Police and Suffolk officials are reticent to remove anything at the memorial, which even includes parts of the car involved in the crash. Teen-agers have written messages on the remains of a tree that the car hit. Candles are lighted each night.
The accident, happening so near the holidays, devastated students at Sayville. That is the main reason the memorial keeps growing. ''People grieve in different ways,'' said John Murphy, the assistant superintendent for Sayville schools. ''We've had additional counselors and support in the school. But there have been at least two services at the site of the crash.''
Some residents question if a memorial of that magnitude is necessary. ''Why do we need this for consolation?'' asked Robert (Bo) Wernersbach of West Sayville. ''We have our families, our churches and our schools. We should be memorializing the memories of the individuals. It's crass to memorialize the site of a tragedy.''
How long the memorial will last is uncertain. The sign received only a temporary permit. ''It has to come down by Jan. 15,'' Mr. Sirico said. As for the rest -- ''at some point someone will have to ask, 'Who has responsibility for that site,' '' Mr. Murphy said. But at least through New Year's Eve, the roadside memorial will serve as a reminder.
Pay-Phone Rules
It used to be that pay phones were a lifeline. Now many are eyesores and used for less than desirable purposes. So, not everyone was shocked when news reports circulated earlier this month that Patchogue had become the first village on Long Island to ban outdoor pay phones.
But it's not true, said Ed Ihne, the deputy mayor of the village. ''We haven't banned them. We're regulating them.''
Outdoor phones in Patchogue now have to go before the pay-phone review committee. ''The person applying has to take a picture of the phone and bring it to the board,'' Mr. Ihne said. ''We review it for appearance and quality of life.'' If the phone passes inspection, the business or person who wants the phone is charged $50 for a pay-phone permit. That person is also responsible for maintaining the appearance of the phone. ''That means no graffiti,'' Mr. Ihne said.
Patchogue officials decided to regulate their outdoor pay phones ''because people were abusing them,'' Mr. Ihne said. ''People were hanging out around them with their beepers.'' Pay phones are becoming instant offices, but it isn't I.B.M. that is moving in. Some of the new offices were being set up for questionable purposes, including drug dealing. ''We tried putting blocks on the phones,'' Mr. Ihne said. ''where people could only call out, you couldn't get incoming calls.''
But it wasn't only the use of the phones. It also was the location. ''Some of the phones were put on village property without the village's permission,'' he said. Thus, the pay-phone permit law. Those without permits will be removed from the village. Now if someone would only remove those pay phones with the unfathomable rates.
Odds and the End
Even an auto body shop can get into the spirit of the holidays. Garden City Cars Inc., which specializes in repairing cars damaged in accidents, is now issuing a gold card. ''It's our way of saying thank you to our customers,'' the owner, Tony Zanello, said. ''It gives you $100 off your next collision, and free towing from your next accident.'' Not to be outdone in odd business promotions, Ben's Kosher Deli has a chicken soup ambulance. The owner of Ben's, who is named Ronnie -- the deli was named for his father, Ben Dragoon -- recently bought an emergency medical truck from the Jericho Fire Department. He repainted the sides to read ''Chicken Soup Emergency Vehicle.'' Painted in bright white and gold (to go with chicken noodle, one would guess,) the vehicle travels the Island, delivering food to customers and the nine Ben's delicatessens. The warning on the rear of the vehicle says, ''Keep back -- hot soup.'' The ambulance isn't Mr. Dragoon's only foray into the medical world. He says, ''We cure our own corned beef.'' Will the financial troubles of the Fire Island Lighthouse be cured by leasing it? The Federal Government believes so. This month it turned over the operation of the lighthouse to the Fire Island Lighthouse Preservation Society. Society officials believe they can raise more money for the lighthouse and thus keep it open more hours.
Photo: Teen-agers at a memorial to two Oakdale youths killed in a car crash. There is concern the memorial could distract drivers. (Maxine Hicks for The New York Times)


text:
Page 1
Down-regulation of proteolytic complexes following EBV activation
in BL cells
Giulia Matusalia, Alessandra De Leoa, Riccardo Gaviolic,
Laura Bertellib, Livia Di Renzob, Elena Mattiaa,*
aDepartment of Public Health Sciences, University ''La Sapienza'', P. le A. Moro 5, 00185 Rome, Italy
bDepartment of Experimental Medicine and Pathology, University ''La Sapienza'', V. le Regina Elena 324, 00161 Rome, Italy
cDepartment of Biochemistry and Molecular Biology, University of Ferrara, Via Borsari 46, 44100 Ferrara, Italy
Received 17 November 2006
Available online 4 December 2006
In Burkitt's lymphoma cells, Epstein Barr virus (EBV) latency products interact with the ubiquitin–proteasome system to promote
episomal maintenance and immunological evasion while the tripeptidylpeptidase II (TPPII) functions as an alternative protease. In
the present study, we have examined the activities and levels of the proteasome and TPPII complex in Raji and in Akata cells after induc-
tion of EBV lytic cycle. The results show that the chymotrypsin-like and caspase-like activities of the proteasome were substantially
reduced in Raji and Akata cells. Similarly, TPPII activity was diminished in both cell lines but was recovered in Akata cells at longer
time after induction. Protein levels of the a/b subunits of the 20S proteasome and TPPII concentration decreased to different extents
after EBV activation, whereas the ubiquitin binding S6' subunit of the 19S regulatory complex increased three to fourfold along with
the levels of ubiquitin-conjugates. Collectively, these observations demonstrate impairment of two major cellular proteolytic systems
at the onset of EBV lytic infection.
? 2006 Elsevier Inc. All rights reserved.
Keywords: Epstein Barr virus; Lytic cycle; Proteasome; TPPII
The Epstein Barr virus (EBV) is an ubiquitous human
herpes virus that replicates in infected cells establishing
either a latent or a lytic infection.
Three types of latent programs have been characterized
depending on the differential expression of a restricted
number of viral genes (reviewed in [1,2]). These include six
nuclear antigens (EBNA1, EBNA2, EBNA-3A, -3B, -3C,
and EBNA-LP), and three latent membrane proteins
(LMP1, LMP2A, and LMP2B). Entry into the viral lytic
cycle is triggered by expression of either of the two EBV
tion as transcriptional activators and initiate the sequential
activation of viral lytic gene expression culminating in
release of infectious viral particles and usually death of the
host cell (reviewed in [3]).
EBV is strongly associated with both B-cell and epithe-
lial cell malignancies [4].
All EBV-associated malignancies have a predominantly
latent pattern of viral gene expression. However, many
EBV-associated tumors are poor targets for the cellular
immune response suggesting that immune evasion strate-
gies might favor tumor development.
The proteasome–ubiquitin proteolytic machinery is
involved in a wide variety of cellular processes that
include cell cycle progression, apoptosis, signal transduc-
tion, transcriptional regulation, receptor internalization,
and the generation of antigenic peptides that trigger
MHC-class I-restricted T cell responses [5]. The protea-
some consists of a 20S cylindrical proteolytic core
that binds 19S regulatory complexes at its ends. The
0006-291X/$ - see front matter ? 2006 Elsevier Inc. All rights reserved.
*Corresponding author. Fax: +39 0649914641.
E-mail address: firstname.lastname@example.com (E. Mattia).
Biochemical and Biophysical Research Communications 352 (2007) 947–952
Page 2
association of these elements forms the 26S proteasome,
which is involved in the targeted degradation of ubiqui-
tin-conjugated proteins [6]. The 20S core is made out of
28 subunits classified as a and b type. The three active
proteolytic sites are contained in the b subunits, b1, b2,
and b5 which exhibit post-acidic (caspase-like), trypsin-
like, and chymotrypsin-like activity, respectively [7].
Following exposure of cells to IFN-c, these subunits are
substituted with new components. The modified protea-
some, known as immunoproteasome [8], is characterized
by a reduced post-acidic activity and by an increased
cleavage after hydrophobic and basic residues. Further-
more, it has been shown to promote a more efficient gen-
eration of T lymphocyte epitopes [9,10].
EBV, like other tumor viruses [11,12], promotes the
maintenance of latency and eludes the host immune
response by interacting with the ubiquitin–proteasome
system. Viral products shown to be involved in this
interaction are the latent proteins LMP2A, EBNA1,
and LMP1 [13]. Ubiquitination of LMP2A and of the
signaling molecules Lyn and Syk affects BCR-mediated
activation of the EBV lytic cycle [14–16]. The long
repetitive sequence of Gly and Ala (GAr) residues on
the amino terminal half of EBNA1 impedes processing
of the protein by the proteasome, affecting thereby the
generation of peptides that can associate with MHC
class I molecules [17]. LMP1 binding to TRAF-2 and
-3 prevents ubiquitin-mediated degradation of these pro-
teins contributing to uncontrolled B cell proliferation
[18]. Moreover, proteasome degradation of LMP1 could
generate peptide fragments of strong immunosuppressive
potential [19].
Modulation of the ubiquitin–proteasome system is also
concomitant to deregulated expression of cellular onco-
genes. In fact, it has been shown that in EBV-positive
Burkitt's lymphoma (BL) cells over-expressing c-Myc,
the enzymatic activity of the proteasomes is largely
reduced. Nevertheless, proteolysis proceeds unperturbed
since de-ubiquitinating enzymes and the serine protease
tripeptidylpeptidase II (TPPII) are highly expressed in
these cells [20].
TPPII is a cytosolic serine protease that removes tripep-
tides from free N-terminus of oligopeptides [21,22]. The
core subunit of about 138 kDa forms large oligomeric com-
plexes that are detected in the cytoplasm or associated with
the plasma membrane of many cell types [22]. It may act
downstream of the proteasomes further digesting partially
degraded proteins [23]; it may participate in antigen pro-
cessing [24] and compensate or replace some proteasomal
function [22,23,25].
At present, it is unknown whether EBV interacts with
the cellular proteolytic systems also during the lytic phase
of infection. To address this question, in this study we have
analyzed the activities and the expression of the ubiquitin–
proteasome and of the TPPII proteolytic systems in two
EBV-positive BL cell lines, after induction of the EBV lytic
Materials and methods
Cell culture and EBV lytic cycle induction. EBV positive BL lines Raji
and Akata were cultured in RPMI 1640 medium containing 5% fetal calf
serum (FCS) and antibiotics, in a 5% CO2atmosphere and maintained at a
cell density of 3.5 · 105/ml.
For induction, Raji cells were treated with TGF-b, sodium butyrate
and P(BU)2 as described [26] and Akata cells were treated with goat anti-
human IgG [27].
Enzymatic assays. At different times, after the addition of EBV lytic
cycle inducers, approximately 5 · 106cells were centrifuged for 5 min at
400g, washed three times with ice-cold PBS and resuspended in lysis
buffer (50 mM Tris–HCl, pH 7.4, 5 mM MgCl2, 250 mM sucrose, 1 mM
DTT, and 2 mM ATP). Samples were frozen at ?80 ?C and thawed
three times before being centrifuged for 10 min at 15,000g. Enzymatic
activities were determined in the supernatant fractions using the fluor-
ogenic substrates (100 lM) Succ-LLVY-AMC (chymotryptic-like), Z-
ARR-AMC (trypsin-like), Z-LLE-AMC (caspase-like) and AAF-AMC
(TPPII) (all from Calbiochem) in a buffer containing 50 mM Tris–HCl,
pH 7.4, 5 mM MgCl2, and 1 mM DTT. Equal fractions of the super-
natants were pre-incubated with the proteasome inhibitor MG132 or
lactacystine (both at 10 lM), or the TPPII inhibitor AAF-CMK at
30 lM (all inhibitors were obtained from Biomol) for 30 min at 37 ?C
before addition of the substrates. The reactions were blocked by
addition of cold ethanol and the fluorescence determined by fluorimeter
(Kontron SFM 25) with an excitation wavelength of 380 nm and an
emission wavelength of 460 nm. The enzymatic activities were adjusted
for the protein concentration of the sample, determined by a modified
Lowry assay (RC DC protein Assay, Bio-Rad).
Western blotting. Raji cells were incubated with TGF-b, sodium
butyrate, and P(BU)2, as described [26]. At the indicated times, samples
(106cells) were collected by a short centrifugation (5 min at 400g), washed
with PBS and lysed in sample buffer (2% SDS, 5% b-mercaptoethanol,
0.001% bromophenol blue, 0.06 M Tris, pH 6.8, and 10% glycerol). To
detect ubiquitinated proteins, cells were lysed in RIPA buffer (50 mM
Tris–HCl, pH 7.4, 1% NP40, 0.1% SDS, and 0.5% sodium deossycolate)
containing 2 mM N-ethylmaleimide (Sigma) to prevent deubiquitination.
Cell lysates were mixed with 4· concentrated sample buffer and sonicated
three times for 5 s at 10 V. Equal amounts of proteins (70 lg), determined
by RC-DC Protein Assay (Bio-Rad) were separated by 10% SDS–PAGE
and electroblotted to PVDF membrane (Amersham Pharmacia Biotech).
Primary antibodies used: anti 20S proteasome a/b subunits (Biomol); anti-
S6' proteasome subunit (Affinity BioReagents); anti ubiquitin (Sigma) and
anti-TPPII (IMMUNSYSTEM). Specific signals detected by chemolumi-
nescence (ECL, Amersham Pharmacia Biotech) were quantified by den-
sitometricanalysisusing the ImageJ
Results and discussion
In EBV-positive Burkitt's lymphoma cells, reactivation
of the EBV lytic cycle can be obtained efficiently with dif-
ferent agents.
Raji cells represent an useful model for study of EBV
activation since a large proportion (>60%) of the popula-
tion becomes positive for the early antigens after incuba-
tion with TGF-b, sodium butyrate and P(BU)2 [26].
However, late antigens expression is blocked and cells do
not lyse [28]. On the other hand, induction of the lytic cycle
in Akata cells by anti-IgG antibodies triggers the full cas-
cade of lytic gene expression with the production of viral
particles, closely reflecting physiological mechanisms which
control the transition from latency to viral reactivation
in vivo.
G. Matusali et al. / Biochemical and Biophysical Research Communications 352 (2007) 947–952
Page 3
In order to study the activity of the cellular proteolytic
complexes following induction of the EBV lytic cycle, Raji
and Akata cells untreated or incubated with lytic cycle
inducers were harvested and lysed. Chymotrypsin-like,
trypsin-like and caspase-like (post-acidic) activities of the
proteasome, and the activity of the TPPII were assayed
using the fluorogenic substrates Suc-LLVY-AMC, Z-
ARR-AMC, Z-LLE-AMC, and with AAF-AMC, respec-
tively. In order to distinguish between specific and unspe-
cificenzymatic activities,
substrates, equal amounts of the supernatants were incu-
bated with MG132 or lactacystine, inhibitors of proteaso-
mal activity, or the AAF-CMK inhibitor of TPPII
activity. The unspecific activity, i.e. that measured in the
presence of each inhibitor, did not vary significantly in
the samples taken at different times of EBV lytic cycle
induction (data not shown). Therefore, it was considered
to be proteasome- or TPPII-specific only the activity sup-
pressible by their respective inhibitors.
The three enzymatic activities of the proteasome were
determined in Raji cells at 24, 48 and 72 h after addition
of EBV lytic cycle inducers and the mean values are report-
ed in the bar-graph of Fig. 1A as percentage of the activity
of control cells. It appears that chymotrypsin-like and cas-
pase-like activity of the Raji proteasome were reduced in
contrast to the trypsin-like activity which remained sub-
stantially stable during induction of the EBV lytic cycle.
In particular, 72 h after induction of EBV lytic cycle, chy-
motrypsin-like and caspase-like activities were reduced to
about 20 and 50% of the control, respectively. Measure-
beforeaddition ofthe
ments carried out in the presence of the proteasome inhib-
itor lactacystine were substantially similar to those
obtained with MG132 (data not shown).
The results observed with Raji cells were confirmed in
Akata cells in which the EBV lytic cycle was induced by
anti-IgG. In this cell line, about 30% of the cell population
became positive for EBV-early antigens when tested by
immunofluorescence (data not shown). As the lytic cycle
goes to completion in these cells, we limited our studies
to the first 24 h of treatment when the majority of cells
(>90%) were still viable. As illustrated in Fig. 1B, chymo-
trypsin-like and caspase-like activities of the proteasome
were significantly reduced upon induction of the EBV lytic
cycle. Evaluation of the inhibitor suppressible activities
indicates that after 24 h of incubation with anti IgG, chy-
motrypsin- and caspase-like activities of the proteasomes
were reduced by 85% and 90%, respectively whereas tryp-
sin-like activity was about 20% lower than that measured
in untreated Akata cells.
For both Raji and Akata cells, a comparison of the
hydrolysis of the fluorogenic substrates that monitor chy-
motrypsin- trypsin- and caspase-like activities of the pro-
teasome suggested that chymotrypsin-like activity was the
main enzymatic function of this proteolytic complex, con-
firming what has been previously observed [20].
Fig. 2 illustrates the results for TPPII activity in Raji
and Akata cells treated with EBV inducers in the absence
or in the presence of the TPPII inhibitor AAF-CMK.
TPPII specific activity of Raji cells (Fig. 2A), was reduced
by 90% during the first 24 h of incubation and remained
Fig. 1. Proteasome enzymatic activities in Raji (A) and Akata (B) cells treated with EBV lytic cycle inducers. Raji cells and EBV-positive Akata cells were
treated with EBV lytic cycle activators as described in Materials and methods. At the indicated times, cell lysates, pre-treated or not with the proteasome
inhibitor MG132, were incubated with the fluorogenic substrates Succ-LLVY-AMC, Z-ARR-AMC, Z-LLE-AMC to evaluate chymotryptic-like, trypsin-
like and caspase-like activities, respectively. The MG132 suppressible activity, considered to be proteasome specific, was evaluated. Data shown in the bar-
graph are the mean values of triplicate determinations carried out at different times after addition of EBV activators to the cells and expressed as
percentage of the activity measured in control cells (time 0) taken as 100%. Standard errors of the mean are shown as error bars.
G. Matusali et al. / Biochemical and Biophysical Research Communications 352 (2007) 947–952
Page 4
similarly low for the entire time frame of the experiment. In
contrast, the activity of TPPII in Akata cells did not show a
significant decrement when tested in cells incubated for
24 h with anti IgG. However, measurements carried out
in cells exposed for shorter times to the inducer (Fig 2B),
showed that TPPII activity undergoes approximately a
80% reduction within the first six hours of incubation
and thereafter progressively increases to the levels mea-
sured in untreated cells. The discrepancy in the results
reported for the two cell lines might be related to the differ-
ent pattern of EBV lytic gene expression in Raji and in
Akata cells. As VCA induction is detected in Akata cells
as early as 6 h post-treatment [27,29], it is conceivable that
TPPII activity is restored during the late phases of the lytic
In order to clarify whether the reduced activities of the
two proteolytic systems measured in Raji and in Akata
cells were dependent on viral activation and not on the
compounds used in the experiments, EBV-negative Ramos
cells were treated with TGF-b, sodium butyrate and P
(BU)2. Proteasomal and TPPII activities were measured
during the first 24 h of incubation when cell viability was
more than 85%. The results obtained showed that protea-
somal activities as well as TPPII activity remained substan-
tially stable over the time frame of the experiment,
indicating that impaired function of the two systems is
dependent on EBV lytic cycle activation (data not shown).
To investigate whether the levels of the proteolytic com-
plexes were also affected by EBV activation, lysates from
Raji and from Akata cells collected at different times after
exposure to lytic cycle inducers, were analyzed by Western
blots with antibodies directed to the catalytic or the regula-
tory subunits of the proteasome as well as with antibodies
specific for the TPPII complex. Fig. 3 illustrates the results
obtained for Raji cells. Polyclonal antibodies recognizing
a/b subunits of the 20S proteasomal core (Fig. 3A) detect
proteins of about 25–30 kDa, whose signal decreased by
different extents during the time frame of the experiment.
At 48 h only the larger component was still detectable
and at 72 h all the bands had virtually disappeared. In
Fig. 3B, a Western blot carried out with antibodies detect-
ing S6'ATPase, a subunit of the 19S regulatory complex is
reported. Unlike that observed for the components of the
catalytic core, the level of this subunit increased about
three-fold during the 72 h following EBV induction.
As ubiquitination is required for degradation of most
proteasomal substrates, we tested whether inhibition of
proteasome activity would affect the clearance of ubiquiti-
nated proteins. Fig. 3C shows a representative Western
blot probed with anti-ubiquitin antibodies in which it
appears a time-dependent accumulation of ubiquitin conju-
gates occurring after addition to the cells of lytic cycle acti-
vators. Quantification of the signals revealed a 3- to 4-fold
increment of ubiquitinated species after 72 h of incubation
(Fig. 3D). Cell viability at this time, determined by Trypan
blue dye exclusion was higher than 80% and sub-G1 events
in cytofluorimetric cell-cycle analysis did not exceed 25%.
These results therefore, rule out the possibility that the
increment of ubiquitinated proteins was dependent on cell
death or apoptosis (data not shown). As the S6' subunit
which binds and directs ubiquitinated proteins to the cata-
lytic chamber, appeared to increase to a similar extent over
the same time, it seems likely that a stimulatory effect is
exerted by ubiquitin-bound substrates on S6' subunit regu-
lation. The recent finding that S6' ATPase activity is great-
ly increased in the presence of ubiquitinated substrates [30]
supports this finding.
Fig. 2. Enzymatic activitiy of TPPII in Raji (A) and Akata (B) cells
treated with EBV lytic cycle inducers. Raji (A) and Akata (B) cells were
treated as described in Fig. 1. TPPII activity was measured in the cell
lysates collected at the indicated times, pre-incubated or not with the
TPPII inhibitor AAF-CMK, after addition of the fluorogenic substrate
AAF-AMC. The AAF-CMK suppressible activity, considered to be TPPII
specific, was evaluated. Data shown in the bar-graph are the mean values
of triplicate determinations carried out at the different times and expressed
as percentage of the activity of control cells (time 0) taken as 100%.
Standard errors of the mean are shown as error bars.
Fig. 3. Western blot analysis of Raji cells after exposure to EBV lytic cycle
activating compounds. Raji cells were treated with EBV lytic cycle
activating compounds as described [26]. At different times, samples of cell
lysates were processed as described in the Materials and methods. The
blots were probed with (A) anti 20S proteasome a/b subunits (B) anti-S6'
proteasome subunit; (C) anti ubiquitin; (E) anti-TPPII; (F) anti GAPDH.
Specific signals detected by enhanced chemoluminescence were quantified
by densitometric analysis. In (D) are reported the densitometric values, in
arbitrary units, of the blot shown in (C). For all blots, one representative
experiment out of three is shown.
G. Matusali et al. / Biochemical and Biophysical Research Communications 352 (2007) 947–952
Page 5
We next evaluated whether decreased enzymatic activity
of the TPPII complex during EBV lytic cycle induction cor-
related with down regulation of TPPII expression. TPPII
antibodies revealed a band of approximately 140 kDa
(Fig. 3E). The intensity of this band transiently increased
during the first 24 h of incubation, but consistently dimin-
ished in lysates of cells exposed for longer periods of time
to the inducers. Hybridization of the blots with antibodies
directed to glyceraldehyde-3-phosphate dehydrogenase
(GAPDH) was used to verify that similar amounts of pro-
teins had been loaded onto each gel track (Fig. 3F).
Western blots carried out on Akata cell lysates gave
results similar to those obtained with Raji cells although
differences in protein expression were less marked, due to
the lower percentage of EBV activation obtained with this
cell line (data not shown).
Taken together, the data here reported indicate that, fol-
lowing EBV lytic cycle induction, the activities of two
major cellular proteolytic systems, the 26S proteasome
and the TPPII complex, are down-regulated in Burkitt's
lymphoma cells, both at the protein level as well as at the
functional level. We show that impairment of proteasomal
activity is accompanied by a specific loss of subunits
belonging to the 20S catalytic core. As the EBV lytic cycle
is triggered by different inducers in Raji and Akata cells,
the results described are likely to depend on EBV activa-
tion. Moreover, since expression of EBV late antigens is
prevented in Raji cells, impairment of the cellular proteo-
lytic systems appears to be an early event upon induction
of EBV lytic cycle. We do not know yet, however, by which
mechanisms modulation of specific subunits of the catalytic
or the regulatory complex occur as a result of EBV lytic
It is well known that cell protein synthesis is blocked by
viral reactivation. It is conceivable that impairment of pro-
tein degradation systems during the early phases of the lytic
cycle might prevent processing of the newly synthesized
viral antigens favoring EBV replication and evasion of
the host immune response.
Forthcoming research aimed at understanding which
viral lytic products interfere with the host proteolytic sys-
tems after EBV activation should have an impact on both
the control of virally infected cells and the development of
new immunization strategies.
This work was partially supported by a contribution of
the ''Istituto Pasteur Fondazione Cenci Bolognetti'', Uni-
versity of Roma, ''La Sapienza'' and by grants from the
Italian Ministry of Education, University and Research
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G. Matusali et al. / Biochemical and Biophysical Research Communications 352 (2007) 947–952


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I love the design. Also I never overheated in the summer or got too cold in the winter. Great purchase!...
English Garden 6-pc Full-size Duvet Cover and Insert Set
Instantly update your bedding with this pretty full-size duvet cover set. The six-piece collection includes duvet, insert, and two shams for a coordinated look. Made from polyester with a soft suede finish, the set promises comfort and durability.
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ITEM#: 13333413This soft suede set includes a reversible duvet cover and shams for twice the decor options. This comforter cover showcases a large scale botanical pattern.
- Set includes: Duvet cover, polyester duvet insert, two shams and two decorative pillows
- Fabric detail: Printed
- Closing: Zipper
- Style: Floral
- Materials: 100-percent polyester
- Care instructions: Machine washable
Full Dimensions
- Duvet cover: 86 inches x 88 inches
- Insert: 86 inches x 88 inches
- Shams: 20 inches x 26 inches
- Decorative pillow: 14 inches x 26 inches
- Decorative pillow: 16 inches square
The digital images we display have the most accurate color possible. However, due to differences in computer monitors, we cannot be responsible for variations in color between the actual product and your screen.
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Perfect all year round in NC
I love the design. Also I never overheated in the summer or got too cold in the winter. Great purchase!Read More
duvet cover set
Very - very happy with this purchase. The entire set is already in our guest bedroom. Colors were right on. She truly does brighten up the room.Read More
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This full-size duvet cover and insert set is made in USA.
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How to Secure King-size Duvet Covers
Wrestling a king-size duvet back into its duvet cover after washing is a battle that can require super-human patience and several extra limbs. The enormous area of king-size bedding usually makes washing or changing your duvet cover a two-person chore, but with a few modifications, you can make switching out your king-size duvet cover much simpler. The easiest solution is to create ties that attach to your duvet insert and keep it secure without holes or extraneous metal parts. For instructions on making this bedding alteration, keep reading.
FAQs about Duvet Covers
If you have a duvet, make sure you have it covered. Duvet covers are essential for duvet owners, and they're a great option if you're considering new bedding. They're one of the most popular bedding options, and so it's smart to brush up on your duvet cover knowledge before making any purchases for your bedroom. Take a look below for some of the most frequently asked questions about duvet covers.
Benefits of Comforter Covers
Duvet covers, also known as comforter covers, are some of the most popular bedding available today, which shouldn't be surprising. These terrific bedroom staples offer a wide range of benefits. Everyone has a different reason for loving comforter covers. Whether you are looking for style, comfort or convenience, duvet covers can help you meet your goal. Comforter covers are available from a large number of trusted brands in a variety of materials, colors and sizes, meaning there's something in the category for everyone. If you are interested in duvet covers but need a bit of convincing before you take the plunge, take a look at the article below, where you will learn the benefits of comforter covers.
How to Secure a Duvet Cover
A duvet cover can protect your comforter from dust, spills, and other dirt, but sometimes getting it on and keeping it in place can be tricky. If you've ever fought to get one onto the duvet or struggled to keep your duvet from slipping around in its cover, then you'll want to follow these tips for securing duvet covers.
Duvet Cover Buying Guide
Whether you call them comforter covers or duvet covers, they are the best thing you'll find to keep your comforter (or duvet) stylish as well as clean. Duvet covers are relatively inexpensive, come in a wide variety of styles and colors and can keep your bedding protected and looking great. This duvet covers buying guide provides information on how to buy duvet covers and how to choose the one that is right for you.
How to Maintain Luxury Duvet Covers
One of the best things about duvet covers is that they allow you to keep your duvets fresh and clean without much fuss. Of course, the covers need some maintenance, and the more luxurious they are, the more careful you may need to be. Fortunately, keeping them clean and feeling great isn't difficult. Here's how to maintain your luxury duvet covers.
Popular Duvet Cover Styles and Fabrics
Duvet covers are available in a variety of fabrics and patterns. The right style for your bedroom is a matter of personal taste. When choosing a duvet cover for your comforter, pick out a style that will go well with the furniture and flooring of your bedroom. Duvet covers should also complement the art on your bedroom walls and the window dressing.
What You Need to Know about Duvet Covers
Changing the look of your whole bedroom is easy when you use a duvet cover. Sometimes referred to as comforter covers, they are the best thing you'll find to keep your comforter or duvet stylish as well as clean. Here's what you need to know when you're shopping for one.
How to Put on a Duvet Cover by Yourself
Putting on a duvet cover is a fast, simple process, even though it may seem potentially troublesome. If you do it correctly, the duvet will lie flat inside the cover, free of wrinkles and bunching. All the process takes is some pulling and shaking; no tricky folding or awkward stuffing is necessary. Fasten the buttons or snaps, and your new duvet cover will be ready for plenty of use. Look below to learn precisely how to put on a duvet cover by yourself.
Duvets vs Down Comforters
A new duvet or down comforter is a fast, easy way to change the look of your bedroom decor. These bed coverings can also keep you warm and toasty on a cold winter's night. Traditionally, the word duvet, a French word meaning "down," was interchangeable with down comforter. Today, however, when most Americans say the word duvet, they are actually referring to a duvet cover. Duvet covers are made to slip over many different types of comforters, whereas a down comforter can be used as a standalone bed covering. Take a look at this article to learn more about the differences between duvets and down comforters.
Best Duvet Covers for Each Season
For those who use duvet blankets year-round, duvet covers allow you to change patterns and color to match every season. Along with the decor of the bedroom, a duvet cover may enhance the ambiance of the entire room or supply it with rich colors and textures for the ultimate comfort factor. If you purchased an expensive duvet, covering it throughout the year will keep it in good shape and allow you to wash it without compromising the batting. Look below to learn the best duvet covers for each season. Spring: Once the evenings start warming up, lighter fabrics like satin provide sheen and style to any bedroom. Satin duvet covers are most often comprised of fabrics like nylon, rayon or silk to provide durability as well as comfort. For spring, lighter colors, including pastels or variations of cream and beige, brighten the room. Summer: During warm summer nights, cotton duvets are one of the most common fabric choices. Light and airy, cotton is also easy to clean and comes in virtually every color and pattern. For the summer season, try a white duvet cover, which will make the room appear more open and spacious. A cotton blend that contains polyester is also an ideal choice for summer, because it is soft, requires simple washing and does not wrinkle. Fall: For the fall season, a duvet cover that is warm enough for intermittent chilly nights is an ideal choice. Microsuede duvet covers offer a soft feel to bedding and come in a variety of colors. This material is easy to care for and machine washable. For fall, choose earthy colors like beige, brown, dark green or burgundy for a seasonal look. Winter: A luxurious duvet cover is a favorite option for warmth during the winter months. Velvet is a soft fabric that comes in a variety of rich colors and provides plenty of insulation for cold nights. The luxurious texture looks good in any style of room decor. For winter months, choose deep, rich colors like burgundy, plum, forest green or chocolate to enhance the room. View All Duvet Covers Back to Guides Directory View All Duvet Covers
How to Buy Futon Covers
Futons are typically covered with decorative futon covers to help keep them clean and undamaged. Futon covers come in all kinds of styles, colors and sizes. Whether you're shopping to replace a worn-out cover or looking to change the decor of a room with new and different colors, there are plenty of futon covers available to suit your style.
Organic Bedding Fact Sheet
Organic bedding sets and accessories offer a wealth of benefits for people who want to live a more environmentally friendly lifestyle while keeping allergies at bay. When you're decorating an eco-friendly sleeping space, you can choose from several styles of organic bedding made from all-natural fibers and synthetic-fiber blends. Whether you're looking for a new duvet cover for the guest bedroom or a complete organic cotton bedding ensemble for the master bedroom, there are several reasons to choose an organic bedding set. This organic bedding fact sheet should help you learn more about it.
How to Create Ambiance with Hotel Collection Bedding
For a bedroom that makes you feel like you're on vacation, accessorize with duvet covers and bedding sets from Hotel Collection bedding. Classic bedding sets from Hotel Collection bedding will enable you to channel the quiet luxury of grand hotels and relax in a bedroom that feels like a getaway. A few simple steps will allow you to create a serene space, so keep reading to learn how to create ambiance with Hotel Collection bedding.
Essential Camera Case Features
When you've invested in a camera, protecting it with a durable camera bag is just good common sense. A good camera case or backpack will protect all your camera accessories and gear without being overly bulky. You can find camera cases in almost every style and size, from simple hard camera-cases that fit in your pocket to full-sized camera backpacks. Whichever type of camera case fits your needs, check for these essential camera case features to be sure you have the basics covered.
How to Buy Computer Speakers for Entertainment
Entertainment and gaming PCs benefit from having powerful, low-distortion computer speakers. Computer speaker systems won't put out the kind of sound you'll get from full-size home theater speakers, but they will definitely upgrade your experience playing games or watching movies when compared to standard factory speakers. Here are a few basic features to watch out for when comparing computer speakers for entertainment purposes.
Tips on Choosing Laptops for Students
When considering laptops for students, it's good to keep in mind three main factors: size, operating system and computing power. The right laptops for students should be able to support any programs they need for school work while still being portable. A bulky and overpowered laptop does not serve an English student very well, while a student studying computer engineering will probably find it essential. Here are a few tips to help you know what to look for in laptops for students.
How to Fold a Twin Blanket
Twin blankets are convenient for foldingand are the ideal bedding size for small spaces. Folding a twin blanket is typically a one-person job. With larger queen and king bed covers, two people are needed to fold the blanket properly. Additionally, smaller covers take up less space than larger quilts or other bedding. It is important to fold a twin blanket as soon as the dryer completes its cycle. There will be fewer wrinkles, and the fresh scent will remain longer. Look below to learn how to fold a twin blanket.
Best King Bedspreads for Summer
When summer comes, you won't want a king bedspread that weighs you down. Commonly used as a term to encompass several types of coverlets and lightweight blankets, bedspreads add a finished look to your bedroom without the added bulk of plush cotton comforters and down-filled duvets. King bedspreads allow you to get a restful night's sleep without sacrificing style. Finding summer bedding for your king sized bed is a breeze with this guide, so keep reading to learn all about the best king bedspreads for summer.
Rolex FAQ
FAQs about Pre-Owned Rolex Watches | Overstock.comFAQs about Pre-Owned Rolex Watchesby Shawna Edwards I've never made a large purchase online. Why pick Overstock.com?Overstock.com is one of the largest retailers of high-end jewelry and watches. We have taken the time to create a Web environment that caters to our customers by allowing them to comfortably shop for wristwatches from the privacy of their homes. We offer our customers a wide variety of timepieces at the most competitive prices available. In addition to this, we want them to feel secure purchasing from us. Overstock.com is one of a handful of online high-end watch merchants that are capable of processing your credit card for your entire purchase. This allows "first-time buyers" to feel more secure in making a large purchase because they have an added layer of security via their credit card company. Do all your Rolex watches come with their serial numbers?Every Rolex watch sold by Overstock.com will have its unique Rolex serial and reference numbers in place and viewable by removing the watch band from the six o'clock and twelve o'clock ends. What is meant by "Perfect Like New Condition?""Perfect like new (day one) condition" watches are pre-owned Rolex timepieces that are purchased by Overstock.com in pristine, mint and immaculate condition. This obviously is the case cosmetically and mechanically. Every timepiece is thoroughly inspected to ensure that they are authentic and meet our quality standards. These luxury watches are so pristine that, in most cases, they are indistinguishable from new watches. Our men's watches and women's watches may have miniscule markings, and the watch bracelets will have miniscule amounts of stretch. Will my watch come with documentation?Every watch will come with detailed documentation stating a full watch description, serial number and model number. Being that the manufacturer's warranty has expired, you will receive a limited one-year warranty from our vendor. Will my watch be covered by a warranty, and what about post warranty service?Being that the manufacturer's warranties on these timepieces have expired, our vendor issues a one-year limited warranty on every watch. The warranty work is done by expert watchmakers who use only original manufacturers' parts and have decades of experience working with Rolex watches. After the one-year limited warranty has expired, you may continue to request service through our vendor who will service the watch when needed at industry cost prices. How can I be sure that the watch will fit my wrist?All our watches will fit up to a 6-and-a-half-inch wrist for a woman and for a man will fit up to a 7-and-a-half-inch wrist (extra links are available for additional charges). Rolex watches can be easily resized to fit any wrist by your local jeweler. How do I measure for my wrist size?You can get an approximate measurement of your wrist by using a soft measuring tape or measuring the inside of your best fitting watch. If you don't have a soft measuring tape, you can wrap a ribbon or a piece of string around your wrist at the wrist bone, mark the spot where it meets, and measure it against a ruler to determine your approximate wrist size. To get the best fit, measure fairly snug at the wrist bone. This is approximate sizing not a 100 percent guarantee of prefect sizing.If the watch is a gift and you do not know the recipient's wrist size, you can use the following as a guide (as with all products, the standard sizes depend on the manufacturer):Men's Standard Bracelet SizesInside, including watch face while bracelet is closedAdult Small5-1/2" to 6-1/2"Adult Average6-1/2" to 7-1/2"Adult Large7-1/2" to 8-1/2"Adult X-Large8-1/2" to 9-1/2" Women's Standard Bracelet SizesInside, including watch face while bracelet is closedAdult Small4-1/2" to 5-1/2"Adult Average5-1/2" to 6-1/2"Adult Large6-1/2" to 7-1/2"Adult X-Large7-1/2" to 8-1/2" Will the watch be fully water-resistant? And can I go swimming or shower with my watch?Every Rolex watch is fully tested for water resistance; the total depth rating is listed with every model. Drawing on years of experience, it is our recommendation that, though Rolex watches are water resistant, because of the fine nature of the timepiece, we discourage our customers from making a practice of taking them in the shower or pool. If for some reason the stem, which is the round mechanism found on the side of the case, which is unscrewed to set the time, is not completely screwed down, water could leak into the movement and damage the watch. (Please refer to your warranty card regarding water damage.) The chances are incredibly slim that this will happen, but nonetheless it is our recommendation that our customers refrain from taking their Rolex watches into the water to avoid any unnecessary problems. How does Overstock.com offer such great prices when my local jeweler has a similar piece for considerably more?Overstock.com has built the best connections in the business, affording us the ability to buy from dealers at the highest level of the industry. Through these connections we can buy in the largest quantities while getting the best pricing. Being that we are not an authorized dealer of Rolex or affiliated with Rolex, USA we are able to offer our customers rock bottom pricing without being subject to any manufacturer's price controls or policies. Do I need to be present to sign for the package?For your safety, you will need to sign for the package that contains your watch. If you are not present when it arrives, the package will not be left at your door. A door tag will be left to inform you when the courier will come to redeliver your package. Alternatively, you can call the courier to arrange a better delivery time or pick-up (with photo identification) at the local hub. What is the benefit of buying a pre-owned Rolex over a new one?Unlike new Rolex watches, pre-owned Rolex watches do not depreciate in value immediately after purchase. Therefore, buying a new Rolex watch is like buying a brand new car: The moment you drive it off the lot it has lost 20 to 30 percent of its value. With buying a pre-owned watch, you have effectively cut out any value depreciation and retail markup. Rolex watches hold their value over time; in most cases, a pre-owned Rolex can be resold at a price very close to your purchase price.Rolex designs don't change too often; therefore, owning a perfect condition pre-owned timepiece is as good as a new one, with no one knowing that the watch was purchased pre-owned. No one can spot a used Rolex on your wrist. With the proper care and maintenance, a Rolex lasts forever. How do I determine the age of my watch?You are guaranteed to receive a desirable modern watch with a fully automatic self-winding (or quartz movement depending on your purchase), with quickset date feature. 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Member Since: August 05, 2011
[6] February 11, 2015 at 10:08am
Some would say that it's foolish to go deep into the forest
that they should stay on the trail.
Not only that …MANY Muslims are coming to Christ, though this does not make for mainstream news
[13] February 11, 2015 at 9:07am
To ALL followers of Christ …
please be in prayer for the persecuted, there are many scattered across the globe.
I believe that the prayers of the saints ( believer) are part of the sustaining power this woman felt.
Blessings …
[18] February 11, 2015 at 9:01am
If a candle is not lit … darkness rules and remains
February 11, 2015 at 8:22am
God made all people … we are ONE race
He does NOT call his people to eliminate anyone because of "race".
Maybe you should check out the numbers killed by Muslims and secularists and the reasons.(David Barton just made a factual statement in response to Obama's nonsense about the crusades.
Gracious, your thinking is backwards and utterly incorrect.
February 10, 2015 at 2:24pm
He just is not an "extremist"
I could be wrong, however.
[8] February 10, 2015 at 2:19pm
I did not say that success was good …!
February 10, 2015 at 2:13pm
So your mind is closed to anything another says …
In reply to Keres's comment on the story Did You Catch the 'Satanic' Religious Theme That Popped Up During the Grammys?
[1] February 10, 2015 at 11:08am
Yo gdibig:
Why then does Jesus refer to some has having satan as their father, that they are children of him …?
We are born in our sin, separated from Christ. When we accept Jesus as Lord AND savior we are then grafted into the family of God becoming His children receiving an inheritance. If not family … NO inheritance.
We are ALL created by God but we are NOT created as family .
I believe you need more study and reading of your scripture and encourage you to do so ….Peace
February 10, 2015 at 10:50am
We are also told to test the spirits … the Lord is not leading if you have not given him the reins … sounds like she is riding her own horse … and her shows are laced with demonic images and references
If God really had her she would clean her act up because the Lord's first words would be: "Katy, why have you abandoned me?"
[17] February 10, 2015 at 10:10am
He is a Muslim following the communist agenda …
[-4] February 10, 2015 at 10:06am
Yo Bren5102:
speak for yourself …the man said he wanted to fundamentally change America ….. what did you expect …!
He is not "ill prepared" … he has been one of the most successful presidents we have had … at least in modern times.
February 10, 2015 at 9:56am
they sure do emulate him … weird ay …
In reply to JamesCrow's comment on the story Did You Catch the 'Satanic' Religious Theme That Popped Up During the Grammys?
[2] January 23, 2015 at 1:58pm
Yo Cubefarmer:
The baby did have a trial …"CrazyTravis" was the judge and executioner.
TO Crazy Travis … how about this … the rapist has NO right and NO claim to the child … it is by fact not "his" baby. The mother here has ALL rights to do as she pleases once the baby is born.( The child's RIGHT!) That is to keep and raise it as her own or give it up for adoption. All fees charged are given to her, and the government provides all costs in bringing the impregnation to birth if she decides to provide for adoption.
In reply to cubefarmer's comment on the contribution Yes, I am Pro-Life Even in Cases of Rape and Incest. Here's Why.
[1] January 23, 2015 at 1:38pm
To Mr. "duh",
Life, if ( and the "if" is for your sake) is created by God and God hates sin … He/it is NOT rewarded … duh …
In reply to ConfederateWoman's comment on the contribution Yes, I am Pro-Life Even in Cases of Rape and Incest. Here's Why.
[5] January 23, 2015 at 11:35am
Actually I know a cop whom is a preacher … or is he a preacher whom is a cop.
Being a cop helps his preaching
and without a doubt his "preaching" makes him a better cop.
[1] January 23, 2015 at 10:58am
Obama does lie … continually ( his own words and actions convict him).
he is anti American ( his own words … " fundamentally change" = AMERICA")
Some, and it seems that you are one , have a problem with the truth.
Obama could have and could be busy about seeking the release of this man without the "public show" of his "concern" and succeeded with his release with humility and honour.
And the man with no hand asked the blind man how many fingers he was holding up …
October 19, 2014 at 1:58pm
Yo ERROR:
Speculation is your specialty … John says he was an EYEWITNESS , and by the way Papias doesn't refute Christ
I go for common sense and HIGH probability …
What you said in your last post does not fall short of TOTAL BS … I have already addressed in simplicities, but you are to smart for … "knowledge puffs up a man"
I waste no further time … I keep hoping …
but, so be it …
[7] October 19, 2014 at 8:32am
Blink has been challenged and refuted and corrected , ah, let's see … counting …. wait …. I have to take my shoes off ….. still counting …. hey dog … come here … dam … where's the kids …. dag, I think I need to go back to college …………………. still counting …. I'll get back to you after I finish the course.
[-1] October 19, 2014 at 8:16am
Just what I was going to post …Ecclesiastes
He said what you say when you are going to do something God says don't do.
So much for the "rock".
Hey "Snooop1e" where are you ….?
October 19, 2014 at 7:52am
I AM CHALLENGING you on the truth of scripture and pretty much everything you have said.
I have no fear of you poking any holes in my understanding … the world view is yours… and I have indicated that I have read that view … it's hard not to as it's everywhere and that is why is it call the world view … you didn't even get that properly stated.
Concerning Papias … I did some reading … as you suggested … NO view damaged here …
What I found … there is almost NOTHING of his OWN writing to have survived, only others that have referred to it such as Eusebius. May have know Polycarp who sat under the ministry of John.
All these men have some things right and some things wrong
Papias does not challenge the truth of Jesus
I will stand corrected any place I am incorrect
I am 4truth …!
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Waseem A Malla
Waseem A Malla belongs to a picturesque hamlet - Radbug, - in Kashmir. He has made attempts with different genres of poetry in English & Urdu, and gets inspired by the works of Mirza Asadullah Khan Ghalib, Mewlana Jalalud Din Rumi, Agha Shahid Ali, Parveen Shakir and Ahmad Faraz. Superficially romantic in nature, his poems depict a thrust on mystical elements, with a representation of Asian ... more »
Click here to add this poet to your My Favorite Poets.
Waseem A Malla Poems
The Special Girl
Every tick of the clock, every beat of my heart, Reminds me of your beauty, of your name, Of your smile, your fragrance, my sweetheart, I can do nothing, just exclaim.
The Feast Of Immortality...! ! !
The darkness stays guarding my house, During the blackest of the nights, Guarding the ruined empire of light, The light which now illuminates the path,
The Cruel Mistress: Life
I think of you as a cruel mistress, Landed I'm in utter distress, This insanity, no wine can bring, This pain in my heart, no bee can sting,
Candle & Moth: An English Ghazal
In the far away lands I saw its glare, The candle at which the moths do stare; There I sat with my mates to see- How,
Piper Of The Holy Land: Kashmir
Far away in an unknown corner, God has carved with His own hand, Valleys that sink and mountains that do tower, Kashmir: a holy, spiritual and a sacred land;
The Flowers Of Your Love...! ! !
From the barren lands of my heart, Sere for years, in utter thirst, Do spring the flowers of your love. Those flowers of Daisy- Innocent,
A Poor Single Mother...! ! !
Tonight she decorated another bed, She was the ornament of another night, Tonight some new arms embraced her, And she slept with another man.
In My Eyes- A Ghazal
Her beauty settled a sight, pristine in my eyes, Dreams have turned, after long, green in my eyes; Numerous seas have set forth from my heart,
I Hope: One Day
You will be mine forever, That all along the way, There will be blessings showered, And flowers blossoming, for me.
One day, as I would look back, Into my life lived all the days, The days without you, and the nights, Without any sleep comporting my eyes.
In My Dreams: A Ghazal
Dislike of Hypnos, perhaps, I provoke in my dreams, I wonder who keeps me awoke in my dreams; Dreams of love & of hatred keep me sleepless,
... It Is: An English Ghazal
Look at my love, how grand it is, So green a crop, in Heart's land it is; Of all in existence, God thus spoke: He commands it to 'Be', and it is;
Every Night: A Ghazal
Every wound of heart extends a little deep every night, Pangs of death & of pain, hush up my sleep every night; Miles & miles lie ahead of me before I depart,
Journey Sans Destiny
I had set upon a journey, far and long, For years I travelled with my luck along; A journey, one of a thousand distances,
Comments about Waseem A Mallamore comments »
Atri Majumder
excellent poems, vivid imagery, good use of similes...keep it up
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(March 26, 1874 – January 29, 1963)
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(26 April 1564 - 23 April 1616)
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The Special Girl
Every tick of the clock, every beat of my heart,
Reminds me of your beauty, of your name,
Of your smile, your fragrance, my sweetheart,
I can do nothing, just exclaim.
You are the one, who has my soul,
You give my heart a reason to fly,
You deserve my life, you special girl,
For you I live, for you I would die…
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Cape Fear 2020
Cape Fear 2020: More people, more crimeSubmitted by WWAY on Wed, 02/20/2008 - 6:27pm.READ MORE:
It's a fact of life, the more people in an area, the more crime. It's one unfortunate side of our region's growth. Full story...»
What makes a filmmaker pick Wilmington?Submitted by WWAY on Wed, 02/20/2008 - 6:04pm.READ MORE:
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Pender County to grow 50 percent in next decadeSubmitted by WWAY on Tue, 02/19/2008 - 7:15pm.READ MORE:
Pender County the population is expected to grow by 50 percent over the next decade. Much of that growth is happening in the county's largest community, unincorporated Hampstead. Full story...»
Cape Fear 2020: Looking at NC's incoming demographicsSubmitted by WWAY on Tue, 02/19/2008 - 7:02pm.READ MORE:
Full story...»
Cape Fear 2020: Baby boomers retiring in southeastSubmitted by WWAY on Tue, 02/19/2008 - 6:33pm.READ MORE:
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Cape Fear 2020: New Hanover County trafficSubmitted by WWAY on Tue, 02/19/2008 - 10:36am.READ MORE:
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NC could gain Congress seatSubmitted by WWAY on Mon, 02/18/2008 - 3:37pm.READ MORE:
It's President's Day, so what better time to talk about the future of politics in the Cape Fear region. Particularly, how will our region fit in as we look towards the year 2020. Full story...»
Cape Fear 2020: Will film incentives continue to work?Submitted by WWAY on Fri, 02/15/2008 - 10:22am.READ MORE:
When film scouts look for a location to shoot their movie they take many things into consideration. One is whether the state offers a tax break for filmmakers. Full story...»
Burgaw planning for growthSubmitted by WWAY on Thu, 02/14/2008 - 6:48pm.READ MORE:
More people, more homes, and more traffic that's what Burgaw town leaders are planning for. The growing Pender County town is showing few signs of slowing down. Full story...»
Cape Fear 2020: Teens and drugsSubmitted by WWAY on Wed, 02/13/2008 - 6:07pm.READ MORE:
Drugs use among teenagers is a problem nationwide and it's no different in our area. In today's Cape Fear 2020 report we take a look at the use of drugs among teens now -- and what you should be aware of in the future. Full story...»
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Why Shop Midtown Comics• Big Savings with Advance Previews • Free Shipping on Qualified Orders • Testimonials
Manufacturer/Publisher: (Release Date: 3/6/2013)
This high-quality plastic figurine is based on a panel from page 2 of The Broken Ear. As he tries to find out more about the stolen Arumbaya statue, Tintin looks through a book from his library, Travels in the Americas. The box scene measures 11cm long and standing 11cm tall.
Item #: 1242111 • Diamond code: DEC121812


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September, a notoriously treacherous month for stocks, began promisingly enough with a 1.4% rally last week. That has encouraged those who see the U.S. stock market poised to climb a proverbial wall of worry. Will it?
Our fall lineup is heavy on drama, what with Middle East skirmishes, uncertain oil prices, and a Beltway fiscal melee that promises to be noisier and more orchestrated than anything on Broadway. But the biggest suspense centers on the future path of U.S. interest rates. Already, the benchmark yield on 10-year Treasuries has jumped in four months from 1.6% to 3% as the Federal Reserve considers scaling back its $85 billion-a-month bond-buying bonanza. So it's a little surprising that the consensus thinks—or hopes—unruly rates will go no higher, for now.
Consider the evidence: Barron's recent survey of Wall Street strategists showed nine out of 10 firms see the 10-year yield finishing 2013 at or below the recent 3% threshold. Traders say we have had four months to contemplate Fed tapering and to pare back vast bond stashes, so September's Fed meeting might well mark a short-term bottom for bonds—and a top for bond yields. Yet home-builder stocks haven't perked up after surrendering a third of their value since May, and the Vanguard REIT VNQ -0.14233187047799786% Vanguard REIT ETF U.S.: NYSE Arca 84.19 -0.12 -0.14233187047799786% /Date(1427922000318-0500)/ Volume (Delayed 15m) : 6574561 AFTER HOURS 84.0007 -0.1893 -0.2248485568357287% Volume (Delayed 15m) : 91551 P/E Ratio N/A Market Cap N/A Dividend Yield 2.4325929445302292% Rev. per Employee N/A More quote details and news » exchange-traded fund (ticker: VNQ) is slumping near 2013 lows.
There are, of course, reasons to hope rates will behave in the short term. The fall roster of well-flagged risks could fuel bids for Treasuries. Central bankers also won't want spiking mortgage rates to destabilize the foundation of our housing recovery. Rising U.S. rates have already sucked capital from emerging markets, forcing countries from Brazil to Indonesia to hike rates to defend their currencies even as economic growth founders. Still-benign U.S. inflation, and squabbling politicians who can't seem to energize our economy, leave the Fed to single-handedly prop us up a little longer.
Friday's mediocre jobs report, and downward revisions trimming the recent pace of job creation to just 160,000 a month, eased some of the anxiety over stimulus withdrawal. "The Fed could ease the pain of tapering by lowering the unemployment rate threshold," notes William O'Donnell, co-head of Americas strategy at Royal Bank of Scotland. Or reduce monthly bond buying by just $10 billion "as a nod to weakening conditions in emerging markets and weaker-than-expected third-quarter U.S. growth."
Yet rates had surged this summer long before and far more than any improvement in economic cues. Once the Fed let out the notion of reducing bond-buying, it became harder to stuff that genie back into the bottle. While economic momentum is far from strong, the efficacy of more bond buying, and swelling the Fed's balance sheet indefinitely, also remains debatable. Foreign appetite for U.S. debt has shriveled, and over the past 12 months foreign purchases of Treasuries have declined to $104 billion from $503 billion a year earlier, notes Stephanie Pomboy of MacroMavens. Since the Fed began talking about tapering, foreigners have turned net sellers of Treasuries.
Backing off the pledge to buy bonds indefinitely also marks the first step toward normalizing rates, and we're merely at the start of a long road back. "Treasury positioning has been cut from long to neutral, but is not yet short, limiting the potential for a short-covering rally," notes Binky Chadha, Deutsche Bank's chief strategist, who thinks rate volatility might subside only at higher levels near 3.25%.
Meanwhile, U.S. manufacturing and auto sales are revving up, and the service sector grew to its best level since 2005. Bespoke Investment Group tracks the number of economic reports beating projections over the past 50 days, and that tally has improved from -27 in June to 3 midweek, a sign the economy is starting to surpass recently tempered expectations. No surprise, Wall Street increasingly favors stocks over bonds, even as it continues to expect bond selling to stay mild enough to keep rates tame.
IS THERE A MORE BELOVED food stock on Wall Street than Hormel Foods HRL 0.4397537379067722% Hormel Foods Corp. U.S.: NYSE USD57.1 0.25 0.4397537379067722% /Date(1427922184430-0500)/ Volume (Delayed 15m) : 687109 AFTER HOURS USD57.1 % Volume (Delayed 15m) : 6485 P/E Ratio 24.29787234042553 Market Cap 15012207755.9403 Dividend Yield 1.7513134851138354% Rev. per Employee 464150 More quote details and news » (HRL)? According to Bespoke, it's the S&P 500 component with the biggest increase in buy ratings in 2013, and the maker of Spam meats and Dinty Moore stews has seen shares rally 46% over the past year, trumping 22% for the packaged-food sector. Hormel missed fiscal third-quarter profit targets, but forgiving investors chose instead to fawn over faster overseas growth. Scoff all you want at salty canned meats with a gelatinous glaze, but Spam is an imported American delicacy in countries from Korea to the Philippines.
Spam has such a following in Hawaii that the so-called "Hawaiian steak" is served in burgers and sushi, but you don't have to be Hawaiian to like Hormel. Falling grain costs will boost meat margins. The tightening supply of turkey means pricier birds this Thanksgiving, and better pricing for Hormel's meat products. The well-run company has lifted earnings at an annual pace near 11%, and a debt-free balance sheet allows it to make acquisitions, like buying Skippy from Unilever UNA.AE 0.03854554798920724% Unilever N.V. CVA Netherlands: Amsterdam EUR38.93 0.015 0.03854554798920724% /Date(1427927700000-0500)/ Volume (Delayed 15m) : 4895082 P/E Ratio 21.772699746862102 Market Cap 66728628445.5 Dividend Yield 2.9283329052144875% Rev. per Employee 279977 More quote details and news » (UL).
But how much of that good news is already in the can? As shares surged, its dividend yield has shrunk to 1.6% from 2.8% some years ago. Shares now fetch 22.3 times what Hormel has earned—the richest in 15 years, and above even that for faster-growing stocks like Apple AAPL -0.14465964799485653% Apple Inc. U.S.: Nasdaq USD124.25 -0.18 -0.14465964799485653% /Date(1427922000394-0500)/ Volume (Delayed 15m) : 40154372 AFTER HOURS USD124.54 0.29 0.23340040241448692% Volume (Delayed 15m) : 467065 P/E Ratio 16.633199464524765 Market Cap 724773401250.228 Dividend Yield 1.5130784708249496% Rev. per Employee 2153110 More quote details and news » (AAPL) and Google GOOG -0.9927007299270073% Google Inc. Cl C U.S.: Nasdaq USD542.56 -5.44 -0.9927007299270073% /Date(1427922000525-0500)/ Volume (Delayed 15m) : 1938696 AFTER HOURS USD542.75 0.19 0.03501916838690652% Volume (Delayed 15m) : 19022 P/E Ratio 26.77285803812428 Market Cap 375259048808.941 Dividend Yield N/A Rev. per Employee 1228170 More quote details and news » (GOOG). With Wall Street gorging on Hormel, pickier eaters might wish to graze elsewhere.
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City-County Council to hear anti-RFRA resolution
The Indianapolis City-County Council will take up a resolution Monday opposing the state's new Religious Freedom…
Evening flurries lead to a frigid night
Puppy dumper pleads guilty, receives $10 fine
Gary Lockridge of Franklin will have to pay a $10 fine and $118 in court costs after pleading guilty to animal neglect, Call 6 Investigator…
Experts, police perform underride crash tests
Crash experts and police from around the state gathered Friday to perform side underride crash tests, Call 6 Investigator Kara Kenney reported.
Trustee seeking homes for displaced residents
Warren Township Trustee Vernon Brown said Wednesday he's trying to find homes for dozens of people displaced from an east-side hotel, RTV6's…
Email blast draws election complaints
The Hendricks County Election Board will hear several complaints filed about the Brownsburg Community School Corporation Superintendent Dr.…
Extra crews called in to inspect IPL manholes
A series of manhole explosions in downtown Indianapolis last week has prompted extra inspections of Indianapolis Power & Light…
WATCH: Ed Sheeran sweet wedding surprise
The video " Ed Sheeran Surprises Deserving Wedding Couple " is #trendingUP and you can see it now, on The List !
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Red Bull Music Academy Festival New York 2015 Announced
Returning to New York City for a third consecutive year, the Red Bull Music Academy is proud to present Red Bull Music Academy Festival New York. The month-long New York festival, which represents the ongoing legacy of the world-traveling Red Bull Music Academy that set up shop in NYC in 2013, brings a program that explores and celebrates the city's rich cultural heritage while spotlighting the modern music landscape. The festival will put on a multifaceted program throughout the month of May, presenting live performances, club nights, lectures and workshops at some of the city's most stunning venues, featuring local innovators, international trailblazers and long-standing members of the Red Bull Music Academy family. Read Full Story
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"HB's review of The Lost Wife"
Yes - though the backdrop is a subject dealt with hundreds of times, the structure of the narration in first person singular of the two main characters was very interesting as was the style of narration and the voice modulation signifying the mood & the environment.
I can not pick one as there were many but some that stood out were Lenka's digging her heels on staying with her family and not going with Yosef unless the other 3 members of her family also come despite opposition from everyone.Then there was the resistance through the drawings & paintings - improvising with what was available and passing the skill to the children so that they could tell the world the truth.Gotlieb proposing to Lenka - taking her back and giving her a good complete life.After liberation Lenka going to Lutia's home and the exchange between Alichka & Lenka.
Just one word Excellent!
Somewhat like that. I finished it pretty quickly.
The depth, visulization, use of words, describing the moments, feelings, pain, suffering - superbly done.
Easy to include myself in the story. Entertaining, emotional, educational. Loved it & will definitely read "Smuggled". I recommend this book.
"Love story set against a sad period of time"
Very engaging book. I'm fascinated by novels set in this period of time because it shows the resilience of people in the worst of circumstances. This book does not disappoint. The characters are developed beautifully through the past and present events. The two narrators of main characters really brings a sense of intimacy with the protagonists. Lovely love story!
"Good read"
The narrator was a little pathetic at doing a male voice but overall did a good job. The story itself kept my attention and I couldn't wait for the book to reach the climax... Unfortunately, I found the ending lacking and disappointing. Kind of like when you work hard on something and then just quit at the end instead of finishing strong.
"Wanting more."
Well, written. Story was good, but the ending is poor. It leaves you hanging and wishing to hear "the rest is the story".
"Historic, yet romantic too"
Loved this story. It was so well read as well, hard to take time for errands, as I wanted to go on a road trip just so I could continue listening w/out interruption!
"A gripping story mixed w/ true historical events!"
I would recommend this story to those that are curious about the true but devastating history of the Holocaust and how Jewish people were treated in the camps, as the author paints a very vivid depiction of what the main characters endure on a daily basis. The main characters tell their story of love, loss, family, and close friendships during the most horrific time of their lives.
Lenka is my favorite character for her strength, endurance and determination, struggling and surviving through the Holocaust.
First of all I love that there are two different narrators, one for Josef and one for Lenka. I could hear the emotions in their voices.
I can't think of a title to rename it to, but maybe to add Terezin or World War II something like The Lost Wife of Terezin.
I love this book!!!
"great story, great narration!"
great story -human struggle and spirit surviving a horrific time in our history
narration was well done and appropriate
A bitter sweet love story that spanned across time, historically based, it has made an impression never to be forgotten.


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- Costs Per Crash
The Federal Motor Carrier Safety Administration released an extensive study of the actual costs of crashes involving many types of trucks - including medium and heavy duty vehicles. The numbers are staggering.
- Posted April 23, 2010
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Follow these steps to ensure safety after an accident
- Posted March 1, 2008


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Away in a manager
Tunica Academy elementary students, under the direction of Melody Horn, presented their annual Christmas program on Tuesday, Dec. 17, at the school.
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Making disciples for the 21st century.
3520 Bishop Rd
Rocky Mount, NC 27804
Church on the Rise is a body of believers dedicated to worshiping the Lord in Rocky Mount. We now have a second location to serve the Nashville, NC community. Our service times are as follows.
Sunday Morning Celebrations:
Rocky Mount Location - 10:20 a.m. with Free Starbucks Coffee from 9:45 a.m. - 10:20 a.m.
Nashville Location - 9:20 a.m
Wednesday Night Connection - 6:45 p.m.
Sunday Night Life Groups Meet at 6:30 p.m.
We are a contemporary church that has four core values:
1. Worship God with all our hearts, soul, mind and strength.
2. Make disciples reach people everywhere with love and knowledge of Jesus Christ.
3. Demonstrate Community sharing life together and reaching out beyond our four walls.
4. Establish Churches to expand through satellite campuses.
Come worship with us!
Category: Wedding Guests|Wedding Guest Accommodations|Churches
the way by sammy - 10/10/2010
i have been attending church on the rise for nearly a year and this is the church that fits me , i prayed for a church home and GOD sent me here, if you do not have a church please visit , you will be welcomed and the son of GOD will never let you down. love to all Sammy
Serving: Rocky Mount NC
Denomination: Baptist Churches, Pentecostal Churches, Assemblies of God, Evangelical Churches
Service: Christian Education, Bible Study, Ministers, Church Consultants
Products: Bibles
Services: Fellowships, Meetings
DexKnows Mobile profile for Church On The Rise in Rocky Mount, NC
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Mississippi Queen......! Hoooo Yaul....!
the air conditioner, 90 degrees and 85% humidity
Currently listening to the thunder,lightening,and rain from a much needed thundershower here in central MO.
Kris Valloton from Bethel - Redding.
Right now some Loreena McKennitt,,, she has an amazing voice and outstanding musicians in her group. Not the normal stuff I listen to, but a refreshing change of pace.
Somebody has to speak for these people. Y'all got on this boat for different reasons, but y'all come to the same place. So now I'm asking more of you than I have before. Maybe all. Sure as I know anything, I know thisthey will try again. Maybe on another world, maybe on this very ground swept clean. A year from now, ten? They'll swing back to the belief that they can make people...better. And I do not hold to that. So no more runnin'. I aim to misbehave. Mal Reynolds Serenity/Firefly
That is a good piece,, from a Tennyson poem I believe,, she also did an excellent job with Noyes' poem " The Highwayman " also,," Caravanserai" is rather appealing to me, she does have an eclectic mix of influences in her work, good stuff.
I hope I shall possess firmness and virtue enough to maintain what I consider the most enviable of all titles, the character of an honest man.
~George Washington
Being an Ex-Music Teacher and one time Jazz-Rock Fusion/Prog. Rock Guitar player, as well as a one time station eng./Broadcaster(back in college after active duty I worked at a college radio station in NYC) listen to and play a wide variety of stuff....right now I'm listening to GATOR eat and drink his lunch.(he slurps like a drunk sailor!!) usually i'll have something on either the Big Ent. center. Hooked up to my TV in the "Great Room" or on My iHome stereo Dock in my bedroom...Nights I will read to "the Alice Cooper show" on my local radio Sta.(the only station in the US with a full time rockstar as a Fulltime DJ...)