text:
Christopher Knaus Google+
Christopher Knaus is a reporter for The Canberra Times.
Woman who allegedly faked domestic violence in fraud loses bail bid
Christopher Knaus Woman who allegedly faked domestic violence to defraud Canberrans loses bail bid despite wanting to show daughter "better way of living".
Canberra Raiders star Josh Papalii in court for driving on suspended licence
Christopher Knaus Canberra Raiders star faces court for allegedly driving on a suspended licence outside a Belconnen pub in the early hours of the morning.
Corrections officer forged reports for sentencing of Canberra criminals
Christopher Knaus A Corrective Services officer forged a string of reports used by judges to sentence Canberra criminals, including notorious double murderer Scott Alexander McDougall.
Canberra killer launches appeal against life sentence
Christopher Knaus The Canberra killer who stabbed mother-of-three Paula Conlon to death is fighting against his life sentence, launching an appeal just one day after his punishment was handed down.
Failing waste company leaves mountain of garbage, multimillion-dollar headache
Christopher Knaus A failing local company has left a mountain of rubbish at a disused depot, potentially leaving ACT taxpayers with a multi-million dollar clean-up bill.
Man smashed with bottle and robbed of phone, throws paint on getaway car
Christopher Knaus A Canberra man was smashed in the head with a bottle, grabbed by the throat, and repeatedly punched by a group who stole his mobile phone last week.
No jail for father who shook his baby causing severe brain damage
Christopher Knaus An intellectually impaired man who shook his crying baby in a tragic moment of frustration, causing irreversible brain damage, will not serve time behind bars.
Man accused of raping his wife, threatening to derail her family's visa application
Christopher Knaus Man accused of raping wife at their Canberra home before threatening to derail family's Australian visa bid.
Man who molested two sisters in his care is facing sentence
Christopher Knaus He took away my childhood, my innocence and my freedom, says sexual assault victim.
Aleksander Vojneski sentenced to life imprisonment for murder of Paula Conlon
Christopher Knaus Violent ice addict Aleksander Vojneski was sentenced to life imprisonment for brutally stabbing to death mother-of-three Paula Conlon in her Canberra home.
Litany of safety failings alleged in Wayne Vickery's death
Christopher Knaus Prosecutors allege safety failings led to fatal grader accident on construction site two weeks before Christmas.
Allegedly murdered Canberra mother Norma Woutersz was found bound with rope in backyard
Christopher Knaus and Michael Inman A Canberra mother allegedly murdered by her daughter was found with significant head injuries and her hands and feet bound with rope.
Senior Rebels bikie allegedly found with ammo cache
Christopher Knaus A senior Rebels bikie was allegedly found with a large cache of ammo, explosive and steroids stored at his Wanniassa home earlier this year.
Murderer Aleksander Vojneski in prison assault case
Christopher Knaus A Canberra man who stabbed his girlfriend to death in her Macgregor home may claim he was mentally impaired during a prison yard attack on a guard a year after the killing.
Gabriela Woutersz, daughter accused of mother's murder in Dunlop is back in prison
Christopher Knaus A woman accused of murdering her mother in Dunlop last month is back in prison after more than two weeks in a psychiatric unit.
Man who spat on police officer's face treated too harshly, court says
Christopher Knaus A man who spat on a female police officer's face had his prison time cut after a court found a seven-month sentence was too harsh.
Bail denied after not guilty plea on drug importation charges
Christopher Knaus A Canberra man allegedly imported huge quantities of drugs using his own name and address. Now he has pleaded not guilty.
Mugga Way murder trial delayed by four months
Christopher Knaus The Mugga Way murder trial of 71-year-old Luigi Costa has been delayed again.
Copa Restaurant pays out two Canberra children, but they won't get money until they are 18
Christopher Knaus The Copa Brazilian Restaurant has been ordered to pay out two children over its mass food poisoning last year, but the pair won't get the money until they are 18.
Barrister left out of pocket after launching doomed appeal for client
Christopher Knaus A barrister has been ordered to fork out for court costs after attempting to start a doomed appeal bid for a client who claims he was unfairly dismissed by the ACT government.


text:
Teresa "Tess" McCabe
Estes Park resident Teresa "Tess" McCabe died at her home on Saturday, November 14, 2009. A complete obituary will be published at a later date. ...Read more ›
Estes Park resident Teresa "Tess" McCabe died at her home on Saturday, November 14, 2009. A complete obituary will be published at a later date. ...Read more ›
Bonnie Lea Howard McCorkhill, born June 10, 1938, beloved wife, mother, grandmother, sister and friend went home to be with the Lord on November 13, 2009. She is survived by the love of her life, Gary Lee McCorkhill, three children: Scott Howard McCorkhill of Morro Bay, California, Tia Ann Noxon (Robert) of Wichita, Kansas, and Randy McCorkhill (Joan) of Bozeman, Montana, and four grandchildren: Bekah and ...Read more ›
Estes Park resident Dr. Harlalee "Hal" Wilson died at Prospect Park Living Center on Sunday, November 15, 2009. He was 83 years old. Harlalee A. Wilson was born February 24, 1926 in Medford, OR. His parents were Lawrence and Pearl Wilson. Hal formerly resided in Iowa City, IA. He moved to Estes Park in 1991. Hal married Sandra Joseph on December 27, 1960 in Hayward, CA. Hal was a veteran of WWII and Korea ( ...Read more ›
Ruth Lewis McClure died Tuesday, October 27, 2009, in Concord, New Hampshire, at the age of 93. Born May 1, 1916, in Athens, Ohio, she was the daughter of Edith Rannells Lewis and Robert Lacey Lewis. A birthright Quaker, she grew up in Sabina, Ohio, and Swarthmore, Pennsylvania. She graduated from Ohio State University in 1939 with a BA in English. She was a homemaker and an investor and lived the majorit ...Read more ›
Jeannette H. Fitton, longtime resident of Estes Park passed away this week. A Memorial Service will be held at 10 a.m. Monday, November 16th at the United Methodist Church of Estes Park located at 1509 Fish Hatchery Road. A reception will follow the service. In lieu of flowers, the family requests donations be made to Rocky Mountain Nature Association or the Estes Park Medical Center. A full obituary will f ...Read more ›
William D. "Bill" Richards, 86 of Estes Park was born in Joliet, IL on January 12, 1923. Bill passed away on October 17, 2009 at the Heart Center of the Rockies, Loveland, CO. He was a devoted father and loving husband to June (nee )'Brien), his wife of 64 years. He is survived by his wife and daughters, Susan Richards of Mt. Kisco, NY, Patricia (Richard) Kwasigroch of Joliet, IL, son Thomas (Debra) Richard ...Read more ›
Myrtha was born July 22, 1946 in Pfafers, Switzerland. She passed away October 21, 2009 at 12:51 p.m. at the Medical Center of the Rockies. Myrtha was very energetic, always walking with friends, and walking with her four legged friend Rocky. She had moved to Estes Park in 1991 from Glenwood, IL. She worked at the high school from August 1997 to April 2003. She was also employee of the month in October 2002 ...Read more ›
Estes Park resident Robert Leiper died at Windsor Healthcare Center on Saturday, October 10, 2009. He was 82 years old. Robert A. Leiper was born August 2, 1927 in Dunbar, NE. His parents were Charles D. and Elizabeth (McClenahan) Leiper. Robert lived in the Pittsburg, PA suburb of Sheridan and graduated from Sheridan High School in 1946. He attended Monmouth, IL Presbyterian College and Xenia Theological S ...Read more ›
© 2014 Estes Park News, Inc

subset: commoncrawl


text:
Touching An Open Scab And HIV Transmission Risk
Here's a taste of what TheBody.com has to offer on this topic:
Ask the Experts
blood on paperwork from hiv/hcv infected indivdualI am pregnant and very worried that a client of mine who is hiv/hcv positive who bled on one of my documents may have caused some form of transmission. he was bleeding from the gums and said so and then he left the clinic. two days later the blood...
Response from Barbara McGovern, M.D.
Tufts University School of Medicine...I would not consider you at risk at all. Read more »
Risk from fresh blood to woundHello dear doc Bob,how are you doing?i hope all is well.I ve written to you in the past when i... Read more »
Re: HIV transmission Risk, WILL DONATE (CASUAL CONTACT, 2010)Hi Doc,I just thought of another question after typing my original ones (below). #4. Would the fact... Read more »
Reducing the Risk of Getting HIV From Sexual Activities...safer sex to riskier sex. Each time you engage in a sexual activity, you choose (whether consciously or unconsciously) the level of risk you are comfortable with for that moment. Safer sex often involves use of latex condoms, latex dental dams,... Read more »
Herpes Zoster (Shingles)...cases of shingles are believed to be a sign of immune restoration syndrome (see Fact Sheet 483 ). Having HIV increases the risk of complications from shingles. These include pain (post herpetic neuralgia.) Also, if you notice any blurred... Read more »
Understanding Genital Herpes...skin-to-skin contact, for example. The virus is spread rarely, if at all, by objects such as a toilet seat or hot tub. Reduce Your Risk of Spreading Herpes People with herpes should follow a few simple steps to avoid spreading the infection to... Read more »
What Are the Symptoms of HIV and AIDS?...difference between the symptoms of HIV and the symptoms of advanced HIV, also known as AIDS. It's what you do that puts you at risk for HIV -- not how you feel. Your mind is a powerful thing. 1.There's a big difference between the... Read more »
Connect With Others
please respond
Posted by jkhjkjkhg, 1 Reply...the park and afterwards, i saw a cm sized wound. now he was at the table about 8-15 minutes before me, and what would be the risk of hiv or any std if there was hiv+ blood on the table, got on his shirt, and that wound (when it was open) touched... Read more »
help? *DELETED*
Posted by pixie, 1 ReplyPost deleted by pixie Read more »
PLEASE respond
Posted by jkhjkjkhg, 1 Reply...the park and afterwards, i saw a cm sized wound. now he was at the table about 8-15 minutes before me, and what would be the risk of hiv or any std if there was hiv+ blood on the table, got on his shirt, and that wound (when it was open) touched... Read more »
scared what if driving me nuts...
Posted by sadman, 3 Replies...transfered to me via the touching after sex? 2: My gums sometimes bleed and we kissed open month a lot could that have put me at risk? 3. Finally I had a cut on the finger next to one I touched her with It was a fresh cut (happened 4 hours... Read more »
Resource Centers
Guides for People With HIV


text:
The present invention relates to transmission-blocking vaccines against malaria. Vaccines of the present invention contain a recombinant Pfs25 Plasmodium falciparum protein produced by yeast cells and to yeast cells producing the protein. Mice and monkeys inoculated with the yeast-expressed Pfs25 of the present invention have developed antibodies with transmission-blocking activity. The present invention also relates to methods of preventing or treating malarial infections using the vaccines of the present invention.
1. A recombinant DNA vector comprising a yeast promoter operably linked to a DNA segment encoding a 25 kDa sexual stage surface protein, Pfs25, of Plasmodium falciparum lacking the amino-terminal secretory signal and hydrophobic carboxyl-terminal region, wherein the protein is capable of eliciting transmission blocking antibodies.
2. The vector according to claim 1 wherein said vector is pBS24.
3. A yeast cell comprising said vector according to claim 1 under condition such that said Pfs25 protein is expressed.
4. The yeast cell according to claim 3 which is AB110.
5. The vector according to claim 1 wherein the DNA segment encodes a polypeptide consisting essentially of amino acids 22 to 190 of Pfs25.
The present application is a continuation-in-part application of the Kaslow et al application Ser. No. 07/658,845 filed Feb. 22, 1991, abandoned, which is a continuation-in-part application of Miller et al application, Ser. No. 07/188,918 filed May 2, 1988, abandoned. The entire contents of both applications are hereby incorporated by reference.
1. Field of the Invention
The present invention relates to transmission-blocking vaccines against malaria and methods of preventing the transmission of the disease.
2. Background Information
The Plasmodium falciparum parasite is the major cause of malaria in humans. The life cycle of the parasite begins in man when young malarial parasites or "sporozoites" are injected into the bloodstream of a human by the mosquito. After injection the parasite localizes in liver cells from which after approximately one week, the parasites or "merozoites" are released into the bloodstream. The entry of the parasites into the bloodstream begins the "erythrocytic" phase. Each parasite enters the red blood cell in order to grow and develop. When the merozoite matures in the red blood cell, it is known as a trophozoite and schizont. A schizont is the stage when nuclear division occurs to form individual merozoites which are released to invade other red cells. After several schizogonic cycles, some parasites, instead of becoming schizonts through asexual reproduction, develop into large uninucleate parasites. These parasites undergo sexual development.
Sexual development of the malaria parasites involves the female or "macrogametocyte" and the male parasite or "microgametocyte." These gametocytes do not undergo any further development in man. Upon ingestion of the gametocytes into the mosquito, the complicated sexual cycle begins in the midgut of the mosquito. The red blood cells disintegrate in the midgut of the mosquito after 10 to 20 minutes. The microgametocyte continues to develop through exflagellation and releases 8 highly flagellated microgametes. Fertilization occurs with the fusion of the microgamete into a macrogamete. The fertilized parasite is known as a zygote that develops into an "ookinete." The ookinete penetrates the midgut wall of the mosquito and transforms into the oocyst within which many small sporozoites form. When the oocyst ruptures the sporozoites migrate to the salivary gland of the mosquito via the hemolymph. Once in the saliva of the mosquito, the parasite can be injected into a host.
Malaria vaccines are being developed against different stages in the parasite's life-cycle including the sporozoite, asexual erythrocyte, and sexual stage. Each development increases the opportunity to control malaria in the many diverse settings within which the disease occurs. Sporozoite vaccines would prevent mosquito-induced infections. First generation vaccines of this type have been tested in humans. Asexual erythrocytic stage vaccines would be useful in reducing the severity of the disease. Multiple candidate antigens have been cloned and tested in animals and in humans.
One type of vaccine being investigated to slow or reverse the worsening epidemic of malaria is a transmission-blocking vaccine [Miller et al., Science 234:1349 (1988)]. Transmission of Plasmodium falciparum from host to mosquito vector can be blocked by monoclonal antibodies against a 25 kDa sexual stage surface protein, Pfs25, expressed on zygotes and ookinetes [Vermeulen et al., J. Exp. Med. 162:1460 (1985)]. The gene encoding Pfs25 has been cloned [Kaslow et al., Nature 333:74 (1988)], and the deduced amino acid sequence revealed a striking feature, the presence of four tandem epidermal growth factor (EGF)-like domains. EGF-like domains are cysteine rich and depend on proper disulfide bond formation for structural integrity [Savage et al., J. Biol. Chem. 247:7612 (1972)]. Of the monoclonal antibodies previously known to block transmission, none recognize the reduced Pfs25 antigen [Vermeulen et al., J. Exp. Med. 162:1460 (1985) and Carter et al., Prog. Allergy 41:193 (1988)], suggesting that for at least some of the blocking epitopes, disulfide bonds are involved in creating proper conformation.
Before Pfs25 can be used as an effective transmission-blocking vaccine, peptides or recombinant DNA-derived Pfs25 molecules having the appropriate conformation for immunological activity in vivo must be produced in large quantities. In addition, adjuvant formulations must be developed which are suitable for use in human transmission-blocking vaccines.
Accordingly, it is an object of the present invention to provide large quantities of Pfs25 or an analogue thereof which elicit transmission-blocking antibodies.
It is another object of the present invention to provide a transmission-blocking vaccine.
Various other objects and advantages of the present invention will become apparent from the following description of the invention together with the figures.
FIG. 1 shows a schematic representation of the natural 217 amino acid Pfs25 protein and the yeast recombinant Pfs25-B. Shown are the four regions with sequence homology to EGF (hatched and arrowed), potential N-linked glycosylation sites ( ), a putative signal peptide sequence (Sig) and consensus signal peptidase cleavage site (↓). The hydrophobic transmembrane domain (TMD) that serves as an attachment signal for the glycosylphosphatidyl-inositol anchor is also shown.
FIG. 2 shows antibody titres of Aotus monkeys immunized with Pfs25-B and MTP-MF59 adjuvant. Monkeys were injected intramuscularly at four weekly intervals (↓).
FIG. 3 shows an immunoblot analysis of Pfs25-B conformation. Lanes 2,4 and 5: Pfs25-B, Lanes 1 and 3: native Pfs25, Lanes 1 and 2: MAb ID2, Lanes 3 and 4: MAb 4B7, Lane 5: antisera P7 MW, prestained protein markers in kDa (BRL).
The present invention relates to transmission-blocking vaccines against malaria. From these studies, it was determined that yeast-produced Pfs25 elicits the production of transmission-blocking antibodies. The present inventors have engineered, by chemical synthesis and mutagenesis, a gene and expressed same in yeast cells to produce an analogue of Pfs25, designated Pfs25-B. Absent from the yeast-expressed protein was the amino-terminal secretory sequence and the hydrophobic carboxyl terminal region. The N-linked glycosylation sites were also removed from Pfs25-B. Pfs25-B was shown to react with conformation dependent monoclonal antibodies despite its conformational changes. In addition, Pfs25-B elicited transmission-blocking activity in mice and monkeys.
The deduced amino acid sequence for Pfs25 is shown in the Sequence Listing, below. The gene has a single exon that codes for a polypeptide of 217 amino acids. The deduced sequence contains a putative signal sequence at the amino terminus and a short hydrophobic anchor at the carboxyl terminus. These regions are identified by the hydrophobic regions noted in the sequence listing.
Accordingly, the present invention relates to recombinant DNA constructs encoding a 25 kDa sexual stage surface protein, Pfs25, from Plasmodium falciparum which lacks the amino-terminal secretory signal and the hydrophobic carboxyl-terminal region. The encoded protein may also lack one or more N-linked glycosylation sites. The DNA segment encoding the protein is operably linked to a vector, for example, pBS24-Pfs25-B. The DNA segment encoding the Pfs25 analogue is exemplified by Pfs25-B gene, however, other genes can be synthesized using methods known in the art.
The present invention also relates to yeast cells transfected with the recombinant construct of the present invention. Suitable yeast cells for use in the present invention include, but are not limited to AB110. Yeast cells of the present invention are grown under conditions such that the Pfs25 analogue is expressed. The yeast-produced Pfs25 of the present invention i purified using methods known in the art, for example, ion-exchange and sizing column chromatography. The product can be used to induce anti-Pfs25 antibodies in mammals, such as mice, monkeys or humans.
The present invention further relates to transmission-blocking vaccines against malaria. A transmission-blocking vaccine prevents the transmission of Plasmodium falciparum from host to mosquito vector. The present invention provides a yeast-produced protein which elicits transmission-blocking antibodies in both mice and monkeys.
Transmission-blocking vaccines of the present invention comprise the yeast-expressed Pfs25 protein or analogue thereof present in an amount sufficient to induce immunization against malaria. The vaccine can further comprise an adjuvant, such as, for example, MTP-MF59 or alum. The vaccine can be administered via intradermal, subcutaneous, intramuscular, nasopharyngeal or respiratory routes, for example, inhalation.
The transmission-blocking vaccines of the present invention can also include other malarial antigens. For example, the transmission-blocking vaccine of the present invention include antigens generating protective malarial immunity.
The present invention also relates to methods of preventing transmission of malarial infections. Methods of the present invention comprise administering to a patient a vaccine of the present invention in an amount sufficient to induce transmission-blocking activity. The treatment consists of a single administration or a series of administrations. When given as a series administrations, inoculations subsequent to the initial administration are given to boost the immune response and may be referred to as booster inoculations.
The treatment given will vary in the number of inoculations and the vaccine used depending on several factors, such as the patient's conditions and the route of administration. These factors are easily assessed by the physician and an appropriate treatment determined therefrom.
The following examples are given to further illustrate the present invention without being deemed limitative thereof.
A Pfs25 gene encoding amino acids 22 to 190 of the natural 217 amino acid precursor protein was chemically synthesized using methods previously disclosed [e.g., Barr et al, J. Biol. Chem. (1988) 263:16471-16478]. The synsthesized gene lacked the proposed amino-terminal secretory sequence of the Pfs25 protein and the hydrophobic carboxyl-terminal region that has been proposed to serve as a signal for attachment of the glycosylphosphatidyl-inositol membrane anchor.
Expression and secretion from yeast (AB110, see Barr et al, J. Biol. Chem. (1988) 263:16471-16478) of the synthesized Pfs25 extracellular domain was achieved by fusion of the synthetic gene to DNA sequences encoding the yeast α-factor pheromone secretory signal/leader sequence [Brake et al, Proc. Natl. Acad. Sci. U.S.A. 81:4642 (1984)]. Transcription of the Pfs25 gene was driven by the glucose-regulated ADH2/GAPHDH hybrid promoter previously described [Shuster et al., In Yeast Genetic Engineering].
A second gene was also constructed, in which the three remaining N-linked glycosylation sites at amino acid positions 112, 165 and 187 of the encoded protein (see Sequence Listing, below) were removed by Asn-Ala mutation and introduction of a termination codon after Glu188. The mutation was carried out using: i) standard M13 mutagenesis procedure (Maniatis et al (1982) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Manual, Cold Spring Harbor, N.Y.), or ii) the polymerase chain reaction. This gene was introduced into a pBS24 plasmid [Barr et al Molecular and Biochemical Parasitology (1991) 47:159-170] using standard methods. The protein produced by the construct was designated Pfs25-B. The removal of the glycosylation sites allowed the secretion from yeast of a homogeneous product that also contained all four epidermal growth factor (EGF)-like repeats described previously for Pfs25 (see Sequence Listing, below) [Kaslow, D. C. Nature 333:74 (1988)].
Yeast cells (AB110) were transformed with the plasmid pBS24-Pfs25-B by the spherophast method [Hinnen et al (1978) Proc. Natl. Acad. Sci. USA 75:1919-1933]. The cells were then cultured/grown as described by Barr et al [J. Biol. Chem. (1988) 263:16741-16478]. The yeast secreted Pfs25-B protein (See FIG. 1B) was purified by gel filtration on S-100 HR (Pharmacia) and analyzed by immunoblotting using rabbit polyclonal antisera to Pfs25 or with the monoclonal antibody (MAb) 1C7 [Kaslow, D. C., Nature 333:74 (1988)], a transmission-blocking MAb that had been shown previously to recognize natural Pfs25 only under non-denaturing conditions.
The purified protein was used to immunize mice (Swiss-Webster) and Aotus trivirgatus monkeys together with the muramyl tripeptide (MTP) adjuvant MF59. The MF59 adjuvant has been used previously to elicit high antibody titres in rodents and goats with other recombinant malaria proteins. MF59 has also been demonstrated to be safe and efficacious in humans when used in conjunction with a yeast-derived human immunodeficiency virus-1 (HIV-1) envelope protein [Abrignani et al., Proc. Natl. Acad. Sci. U.S.A. 87:6136 (1990)].
Mice were immunized with Freunds adjuvant plus Pfs25-B or MF59 plus Pfs25-B. In the Freunds adjuvant group, mice were immunized three times at three weekly intervals with 50 ug of Pfs25-B in a final volume of 100 μl as described previously. In the MF59 adjuvant groups, mice were immunized, using the same protocol with Pfs25-B, falc 2.3, hEGF or hSOD (50 ug per injection). Animals were bled one week after the final immunization and antibody titres were measured in the ELISA format as described previously [see Table 7 below].
Monkeys were injected intramuscularly with three 100 μg doses of Pfs25-B in MF59 at four weekly intervals (see FIG. 2). Titres were measured by ELISA every two weeks and sera taken at weeks 0, 12 and 22 were used for mosquito feeding experiments described below.
Humoral immune responses elicited in mice with both Freunds adjuvant and MF59 (see Table 1 below) were measured by ELISA. Also shown in Table 1 are the reactivities of antisera raised with recombinant Pfs25 against various control recombinant proteins from yeast. These include, human superoxide dismutase (hSOD) [Hallewell et al., Biotechnology 5:363 (1987)], a circumsporozoite (CS) protein from P. falciparum (falc 2.3), and human EGF [Brake et al., Proc. Natl. Acad. Sci. U.S.A. 81:4642 (1984) and George-Nascimento et al., Biochemistry 27:797 (1988)].
TABLE 1______________________________________Antibody titres developed in mice by immunization with re-combinant Pfs25-B and either Freunds (FCA/FIA)or MF59 adjuvants. Assay ProteinAntigen Adjuvant Pfs 25B Falc 2.3 hEGF hSOD______________________________________Pfs25-B FCA/FIA 42,000 <100 <100 <100Pfs25-B MF59 31,625 <100 <100 <100Falc 2.3 MF59 <100 20,200 <100 NDhEGF MF59 <100 <100 9,100 NDhOSD MF59 <100 ND <100 18,750______________________________________ ND = Not determined
The ability of sera to block the formation of oocysts in P. falciparum gametocyte-fed mosquitoes was determined as described previously [Quakyi et al., J. Immunol. 139:4213 (1987)]. The results are shown below in Table 2A.
Briefly, sera from mice immunized with Pfs25-B or falc 2.3 (as described above) were assayed for transmission-blocking activity and compared with the Mab 1D3. Mosquitoes were fed on either the mouse sera or Mab ascites fluid at a 1:2 dilution of the feed [Quakyi et al., J. Immunol. 139:4213-4217 (1987)]. Mosquitoes were dissected after 7-8 days, and the numbers of infected mosquitoes and oooysts determined. Infectivity was scored as the number of oooysts found in the sample divided by the number found in the pooled prebleed control X100%.
Murine antisera against Pfs25-B from either Freunds or MF69-adjuvanted mice were able to dramatically reduce or even abolish the sexual development of the parasite within the mosquito mid gut. Only one oocyst was detected in 37 mosquitoes dissected from the immune sera groups, whereas all 16 control, preimmune sera-fed mosquitoes developed oocysts, with an average number of 8 per mosquito. This is comparable with results obtained using the transmission-blocking MAb 1D3. This MAb, at a dilution of 1:2 of ascites fluid, fully blocked oocyst development in all 16 mosquitoes tested (see Table 2A).
Similarly, sera from two A. trivirgatus monkeys were capable of blocking completely the development of oocysts in mosquitoes after immunization with Pfs25-B. The ability of the sera to block oocyst development was measured as above. Prebleed sera from each monkey (numbers A108 and A142) were used as controls. Transmission-blocking activities were measured at 12 and 22 weeks. Mabs 1D2 and 4B7 were also shown to block oocyst appearance at a 1:2 dilution, whereas sera from the two monkeys that were injected with MF59 alone failed to inhibit oocyst development at any of the bleeds assayed. This transmission-blocking activity persisted for nearly 4 months in one monkey and more than 6 months in the other (see Table 2B below).
TABLE 2______________________________________Inhibition of oocyst development by sera from immunized animals. Infect- Mean Oocyst ivity Sample Numbers (Range) % of Control ##STR1##______________________________________Pooled Preimmune B 100% 16/16 (1-22)Pfs25-B (CFA/IFA) 0 <1% 1/19 (0-1)Pfs25-B (MF59) 0 0% 0/18 (0)Falc 2.3 (MF59) 4 50% 17/18 (0-13)Mab 1D3 0 0% 0/16 (0)BA108 Preimmune 39 100% 20/21 (0-89)A142 Preimmune 25 100% 21/21 (5-75)A108 Week 12 0 <1% 1/24 (0-1)A142 Week 12 0 0% 0/20 (0)A108 Week 22 0 0% 0/28 (0)A142 Week 22 2.6 10% 15/26 (0-35)Mab 1D2 0 <1% 1/20 (0-1)Mab 4B7 0 0% 0/24 (0)______________________________________
Conformation-dependent antibodies did not react as strongly with Pfs25-B as with native Pfs25 (See FIG. 3). Pfs25-B (10 ug per lane) and native Pfs25 (Titron X-100 extract from 5�106 5 hr zygotes per lane), were fractionated by 4-20% SDS-PAGE in the presence (reduced) or absence (nonreduced) of 5% 2-mercaptoethanol and electroblotted to nitrocellulose as previously described [Quakyi et al., J. Immunol. 139:4213-4217 (1987)]. Binding of a 1:200 dilution of MAb 1D2 (lanes 1 and 2), Mab 4B7 (lanes 3 and 4), or antisera P7 (lane 5) was detected by alkaline phosphatase-labeled goat anti-mouse sera (PlcoBlue, Stratagene).
MAB 4B7 was a product of a fusion of spleen cells from a BALB/c mouse immunized twice with recombinant vaccinia virus that were expressing Pfs25 and boosted with whole P. falciparum gametes. Supernatants from the fusion were screened for specificity to Pfs25 by immunoblot of reduced Pfs25-B. Antisera P7 was produced from mice immunized with synthetic peptides derived from the deduced amino acid sequences of Pfs25 [Kaslow et al., Nature 333:74-76 (1988)].
Conformational integrity has been shown to be extremely important for the recognition, by antibodies, of epitopes of Pfs25 that block transmission. Surprisingly, the conformation-dependent neutralizing MAbs 1C7, 1D2 and 1D3 [Kaslow et al., Nature 333:74-76 (1988)] that are known to react with the same epitope of Pfs25 [Quakyi et al., J. lmmunol. 139:4213-4217 (1987)] did not react as strongly with the secreted recombinant Pfs25-B as they did with native Pfs25 on non-reducing SDS-PAGE immunoblots (see FIG. 3). Although the related six cysteine residue-containing hEGF has been shown to be correctly folded and disulfide-linked when using this expression system [Brake et al., Proc. Natl. Acad. Sci. U.S.A. 81:4642-4646 (1984) and George-Nascimento et al., Biochemistry 27:797-802 (1988)], recombinant Pfs25-B with four EGF-like, repeats and twenty-two cysteine residues may possess only partial structural integrity. Pfs25-B also lacked the glycosylphosphatidylinositol anchor that is characteristic of the native Pfs25 molecule [Fries et al., Parasite Immunol. 11:31-45 (1989)].
As the epitope recognized by the conformation-dependent neutralizing MAb 1D2 is destroyed by reduction (see FIG. 3), but not by SDS, varying salt concentrations, or extremes of pH, the reactivity of Mab 1D2 against recombinant Pfs25-B is a sensitive assay for proper disulfide bond formation at or about the Mab 1D2 epitope. The very weak reactivity of Mab 1D2 with secreted Pfs25-B suggests that the 1D2 epitope is not completely recreated in this recombinant protein. Post-translational modification differences could explain these findings. In addition, these findings can be explained by improper disulfide bond formation.
Antisera P7, generated against a group of synthetic peptides of Pfs25 recognizes only fully reduced native protein and, therefore, serves to assay for the complete absence of disulfide bonds. P7 reacts with Pfs25-B on reducing but not on nonreducing SDS-PAGE immunoblots, demonstrating that Pfs25-B contains disulfide bonds. Also, 4B7, a previously undescribed, Mab to Pfs25, that reacts quite strongly with nonreduced Pfs25 and weakly with reduced Pfs25, clearly reacted with nonreduced Pfs25-B with the reactivity increasing markedly upon reduction of Pfs25-B (see FIG. 3). These data suggest that the Mab 4B7 epitope is also improperly formed in Pfs25-B and that the reduced reactivity with Pfs25-B as compared to parasite-produced Pfs25 is not a result of post-translational modification.
Antibodies that recognize reduced Pfs25, such as Mab 4B7, were shown to block transmission (see Table 2), suggesting that epitopes that are not dependent on disulfide bonds for conformation may elicit transmission-blocking antibodies. Despite this, however, the yeast-derived Pfs25-B of the present invention was fully capable of inducing transmission-blocking immunity in both mice and monkeys. In addition, transmission-blocking antisera did not recognize recombinant hEGF in an ELISA assay (see Table 1), nor did antisera to hEGF recognize Pfs25-B in such an assay (see Table 1). The cross-reactivity of antisera to hEGF with Pfs25-B was also below the limit of detection. The homology of Pfs25 to EGF might give rise to an auto-immune response in individuals immunized with Pfs25. Dara, however, indicates that this is not likely to be the case. It is anticipated that this lack of cross-reactivity will be similar for other members of the polypeptide family that contain EGF-like motifs and are, therefore, not likely to be a concern in transmission-blocking vaccination studies with Pfs25.
Several recombinant DNA-derived antigens or synthetic peptides of malaria parasites have been shown to be only weakly immunogenic in humans [Herrington et al., Nature 328:257-259 (1987) and Gordon et al., Am. J. Trop. Med. and Hyg. 42:527-531 (1990)]. Since pfs25 is not expressed at the gametocyte stage, but appears only during sexual development in the mosquito, and has, therefore, not been subjected to immune pressure in the human host, it is anticipated that the immunogenicity of Pfs25-B in humans will closely parallel that observed in experimental animals. Furthermore, transmission-blocking immunity elicited in both mice and monkeys was observed with MTP-MF59, an adjuvant that has been shown previously to be safe and efficacious in humans [Abrignani et al., Proc. Natl. Acad. Sci. U.S.A. 87:6136-6140 (1990)]. Based on the extremely high yields of the Pfs25 protein that can be obtained from yeast, together with the immunogenicity data, the combination of Pfs25 from yeast and MTP-MF59 represents an excellent initial formulation for the study of transmission-blocking immunity in humans.
All references cited hereinabove are hereby incorporated by reference.
While the foregoing invention has been described in some detail for purposes of clarity and understanding, it will be appreciated by one skilled in the art from a reading of this disclosure that various changes in form and detail can be made without departing from the scope of the invention.
__________________________________________________________________________SEQUENCE LISTING(1) GENERAL INFORMATION:(iii) NUMBER OF SEQUENCES: 2(2) INFORMATION FOR SEQ ID NO:1:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 767 base pairs(B) TYPE: nucleic acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: DNA (genomic)(ix) FEATURE:(A ) NAME/KEY: CDS(B) LOCATION: 75..725(ix) FEATURE:(A) NAME/KEY: miscfeature(B) LOCATION: 84..125(D) OTHER INFORMATION: /note="N-terminal HydrophobicRegion"(ix) FEATURE:(A) NAME/KEY: miscfeature(B) LOCATION: 681..725(D) OTHER INFORMATION: /note="C-terminal HydrophobicRegion" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:AATTGTTGTGAAAAGAAAAAAACAAAAAAAAAAAAAAAAAAACTCATACCTTATATTTTT60TTATTCTTTTAAAAATGAATAAACTTTACAGTTTGTTTCTTTTCCTTTTC110MetAsnLysLeuTyr SerLeuPheLeuPheLeuPhe1510ATTCAACTTAGCATAAAATATAATAATGCGAAAGTTACCGTGGATACT158IleGlnLeuSerIleLysTyrAsnAsnAla LysValThrValAspThr152025GTATGCAAAAGAGGATTTTTAATTCAGATGAGTGGTCATTTGGAATGT206ValCysLysArgGlyPheLeuIleGlnMetSerGl yHisLeuGluCys303540AAATGTGAAAATGATTTGGTGTTAGTAAATGAAGAAACATGTGAAGAA254LysCysGluAsnAspLeuValLeuValAsnGluGluThrCysG luGlu45505560AAAGTTCTGAAATGTGACGAAAAGACTGTAAATAAACCATGTGGAGAT302LysValLeuLysCysAspGluLysThrValAsnLysPro CysGlyAsp657075TTTTCCAAATGTATTAAAATAGATGGAAATCCCGTTTCATACGCTTGT350PheSerLysCysIleLysIleAspGlyAsnProValSer TyrAlaCys808590AAATGTAATCTTGGATATGATATGGTAAATAATGTTTGTATACCAAAT398LysCysAsnLeuGlyTyrAspMetValAsnAsnValCysIl eProAsn95100105GAATGTAAGAATGTAACTTGTGGTAACGGTAAATGTATATTAGATACA446GluCysLysAsnValThrCysGlyAsnGlyLysCysIleLeuAspT hr110115120AGCAATCCTGTTAAAACTGGAGTTTGCTCATGTAATATAGGCAAAGTT494SerAsnProValLysThrGlyValCysSerCysAsnIleGlyLysVal125 130135140CCCAATGTACAAGATCAAAATAAATGTTCAAAAGATGGAGAAACCAAA542ProAsnValGlnAspGlnAsnLysCysSerLysAspGlyGluThrLys 145150155TGCTCATTAAAATGCTTAAAAGAAAATGAAACCTGTAAAGCTGTTGAT590CysSerLeuLysCysLeuLysGluAsnGluThrCysLysAlaValAsp 160165170GGAATTTATAAATGTGATTGTAAAGATGGATTTATAATAGATAATGAA638GlyIleTyrLysCysAspCysLysAspGlyPheIleIleAspAsnGlu 175180185AGCTCTATATGTACTGCTTTTTCAGCATATAATATTTTAAATCTAAGC686SerSerIleCysThrAlaPheSerAlaTyrAsnIleLeuAsnLeuSer190 195200ATTATGTTTATACTATTTTCAGTATGCTTTTTTATAATGTAAATATTAT735IleMetPheIleLeuPheSerValCysPhePheIleMet205210 215AACAACATATATATATATTTTTAAATGGTAAA767(2) INFORMATION FOR SEQ ID NO:2:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 217 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein( xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:MetAsnLysLeuTyrSerLeuPheLeuPheLeuPheIleGlnLeuSer151015IleLysTyrAsnAsnAlaLysValThrValAspThrValCysLysAr g202530GlyPheLeuIleGlnMetSerGlyHisLeuGluCysLysCysGluAsn354045AspLeuValLeuVal AsnGluGluThrCysGluGluLysValLeuLys505560CysAspGluLysThrValAsnLysProCysGlyAspPheSerLysCys6570 7580IleLysIleAspGlyAsnProValSerTyrAlaCysLysCysAsnLeu859095GlyTyrAspMetValAsnAsnValCysIleProAsn GluCysLysAsn100105110ValThrCysGlyAsnGlyLysCysIleLeuAspThrSerAsnProVal115120125LysT hrGlyValCysSerCysAsnIleGlyLysValProAsnValGln130135140AspGlnAsnLysCysSerLysAspGlyGluThrLysCysSerLeuLys145150 155160CysLeuLysGluAsnGluThrCysLysAlaValAspGlyIleTyrLys165170175CysAspCysLysAspGlyPheIle IleAspAsnGluSerSerIleCys180185190ThrAlaPheSerAlaTyrAsnIleLeuAsnLeuSerIleMetPheIle195200 205LeuPheSerValCysPhePheIleMet210215
Patent Citations
Non-Patent Citations
Referenced by
Legal Events
Original Image


text:
Lobstein allowed four earned runs in 10 innings this spring. Wilson surrendered 10 runs (six earned) in 6 2/3 innings. Zeid gave up six earned runs in 7 2/3 innings. The moves leave the Tigers with 39 players in major-league camp.
"Those six, it'd be surprising if they weren't on the team," Ausmus said Friday morning. "Something strange would have to happen just based on their performances from the past."
This leaves Alex Wilson, Josh Zeid and Buck Farmer all battling for the final slot in the bullpen.
"You're always concerned about injuries," Ausmus said. "I think that's where the depth comes into play, where, if something unexpected comes up, we have a lot of arms we feel like can produce at the major league level. ... If something happens at any time, we feel that we have a quality arm that we can slide right in."
Cespedes is in the final year of a four-year, $36 million contract, while Porcello is in his final year of arbitration.
The 29-year-old Cespedes has a .263 average, .464 slugging percentage and .780 OPS in 416 games over three MLB seasons. Porcello, who won a career-high 15 games and had a career-low 3.43 ERA in 2014, is 76-63 with a 4.30 ERA in six MLB seasons.
Wilson has a 2-1 record, 3.38 ERA and 1.30 WHIP in 44 relief outings for Boston. He had a 1.91 ERA and 0.88 WHIP in 18 outings in 2014.
The 19-year-old Speier is 3-0 with a 1.64 ERA and 0.88 WHIP in 12 career appearances (eight starts) in the minors. He was a 19th-round pick in 2013.
Betts returns after spending under two weeks at Pawtucket. Wilson also spent a short time with Boston earlier this year, giving up one run on three hits in three innings pitched.
Wilson has been up and down this year. He's appeared in two games, posting a 3.00 over three innings.
Wilson was brought up to give relief to an overworked bullpen Wednesday, but starter John Lackey was able to pitch eight excellent innings and allow most of the team's relievers to get some rest. The move will free up a roster spot for Shane Victorino, who is expected to be activated from the disabled list Thursday.
The 27-year-old Wilson has performed well out of the bullpen this season with eight scoreless innings and nine strikeouts. But he has also walked five, continuing a trend that followed him last year with the Red Sox. He walked 14 in 27 2/3 innings in Boston a year ago.
The club cut down from 58 to 46 players Thursday. Both Ranaudo and Cecchini are considered prospects within the system, but need more time to develop in the minors before they make their major-league debuts.
Wilson had surgery on his thumb in October. He's been throwing the past two weeks. He posted a 4.88 ERA in 27 2/3 innings with the club last year.
Wilson was diagnosed with joint inflammation in early July after surrendering six earned runs in two innings over two appearances. He owns a 4.88 ERA, 1.74 WHIP and 22:14 K:BB ratio in 27 2/3 innings.
Wilson pitched one-third of an inning and allowed a run on two base hits. There is no word as to how many rehab appearances Wilson will need before returning to the Boston bullpen.
Wilson has been diagnosed with joint inflammation and won't throw for at least four days, according to manager John Farrell.
"This is better than we first anticipated," Farrell said. "It was a favorable exam, all things considered."
He's appeared in 17 games for Boston this season with a 2.61 ERA and 1-1 record. He has 17 Ks over 20 2/3 innings.
Wilson was called up Thursday, and tossed 2 2/3 of work during the game. He gave up three hits and one earned run, while striking out two hitters. He will be replaced by Rubby De La Rosa.
Wilson pitched 18 innings for Boston this season, with a 2.50 ERA and 1.56 WHIP. Wilson produced a 5.00 ERA in nine minor league innings.
Wilson was solid for Boston in 16 appearances, as he allowed a 2.50 ERA over 18 innings. However, with the Red Sox needing a position player for their upcoming series at Philadelphia, the club replaced him with outfielder Jackie Bradley.
Wilson appeared in 16 games for Boston with a 2.50 ERA, a 1-0 record and 15 strikeouts over 18 innings.


text:
Live updates
MP calls for 'huge fines for petrol price fixing'
Conservative MP Robert Halfon who has led a campaign calling for a full investigation into alleged cartels and market manipulation in the oil market for the past three years, said:
Last year, in a debate that I pressed for, Parliament voted unanimously for an investigation into the oil market.
These latest allegations underline why that must happen urgently in the UK.
High oil prices are crushing families across Britain. Motorists are being taken for a very expensive ride.
The Government has done its bit, by freezing fuel duty for three years. Now oil companies must come clean and show some responsibility for what is happening to the international oil price.
Mr Halfon told BBC Radio 4's Today programme anyone found guilty of price fixing should face jail and oil firms should expect hefty fines:
Clearly there has been a problem, there needs to be a full inquiry.
The Government needs to say, anyone caught doing this, there should be prison for price fixing and there should be huge multimillion-pound fines on any oil company found guilty and that money should be passed back to the motorist.
'Price fix' raids: A fuel rip-off?
It is possible some of our biggest oil companies have been breaking the rules. If true drivers may have been paying over the odds for years.Read the full story ›
Quentin Willson 'urgent need for powerful watchdog'
The sharp rises in fuel and oil prices we've seen recently have raised huge concerns about what is really driving them.
The oil market isn't transparent or accountable enough. There is an urgent need for a powerful and independent watchdog to ensure that there's no market manipulation and that UK families and businesses pay a fair price for their fuel.
Tory MP: 'Enough is enough' with oil fixing allegations
Conservative MP Robert Halfon said the price of petrol at the pumps has risen by a "huge amount" since 2009.
Speaking to Daybreak he said he warned that petrol price fixing had gone on "a year ago".
He added: "We had a whistleblower approach us, we urged the office of fair trading to do a full investigation, they did a partial Sir Humphry investigation, and we say enough is enough."
EC: Oil price 'distortions' would 'harm consumers'
The European Commission said in a statement that even small distortions of assessed prices could have a "huge impact on the prices of crude oil, refined oil products and biofuels purchases and sales, potentially harming final consumers".
The statement said:
The European Commission can confirm that, on 14 May 2013, Commission officials carried out unannounced inspections at the premises of several companies active in and providing services to the crude oil, refined oil products and biofuels sectors.
The Commission has concerns that the companies may have colluded in reporting distorted prices to a Price Reporting Agency to manipulate the published prices for a number of oil and biofuel products.
Furthermore, the Commission has concerns that the companies may have prevented others from participating in the price assessment process, with a view to distorting published prices.
Any such behaviour, if established, may amount to violations of European antitrust rules that prohibit cartels and restrictive business practices and abuses of a dominant market position (Articles 101 and 102 of the Treaty on the Functioning of the EU and Articles 53 and 54 of the EEA Agreement).
RAC: Oil fixing allegations 'worrying for motorists'
RAC technical director David Bizley said the oil price fixing allegations were "worrying news for motorists" who are already suffering due to the high cost of keeping a vehicle.
He added: "The Office of Fair Trading inquiry concluded at the end of January that the UK fuel market was operating fairly and not against the best interests of motorists, and therefore that a Competition Commission investigation was not needed.
"Motorists will be very interested to see what comes of these raids. Whatever happens the RAC will continue to campaign for greater transparency in the UK fuel market and for a further reduction in fuel duty to stimulate economic growth."
Tory MP: 'Oil companies must now come clean'
Tory MP Robert Halfon has led a campaign calling for a full investigation into alleged cartels and market manipulation in the oil market for the past three years.
The Harlow MP, who founded the www.PetrolPromise.com website to campaign for cheaper petrol and diesel, said:
Last year, in a debate that I pressed for, Parliament voted unanimously for an investigation into the oil market.
These latest allegations underline why that must happen urgently in the UK.
High oil prices are crushing families across Britain. Motorists are being taken for a very expensive ride.
The Government has done its bit, by freezing fuel duty for three years.
Now oil companies must come clean and show some responsibility for what is happening to the international oil price.
Labour: Oil 'fix' reports 'suggest shocking behaviour'
Shadow energy and climate change secretary Caroline Flint said:
These are very concerning reports, which if true, suggest shocking behaviour in the oil market that should be dealt with strongly.
When the allegations of price fixing in the gas market were made, Labour warned that opaque over-the-counter deals and relying on price reporting agencies left the market vulnerable to abuse.
These latest allegations of price fixing in the oil market raise very similar questions.
Consumers need to know that the prices they pay for their energy or petrol are fair, transparent and not being manipulated by traders.
Shadow financial secretary to the Treasury Chris Leslie said:
If oil price fixing has taken place it would be a shocking scandal for our financial markets.
Labour tabled amendments in Parliament last year calling for commodities like oil to be part of the Financial Conduct Authority's regulatory net, but Ministers refused to act.
They should explain why they complacently failed to do so.
Statoil confirms raid at Norway headquarters
Statoil has confirmed a raid took place at its headquarters in Stavanger, Norway, earlier today.
Statoil said the inspection was carried out by the EFTA Surveillance Authority with the assistance of the Norwegian Competition Authority.
A statement on the firm's website said the inspection was carried out "on request of the European Commission".
"Statoil is cooperating with the authorities in their inspection", it added.
BP 'cooperating fully' with EU oil price fix investigation
BP said it is "cooperating fully" with the EU's anti-trust body after it launched an investigation into allegations of oil price fixing.
A spokesperson said: "BP is one of the companies that is subject to an investigation that was announced earlier today by the European Commission.
"We are cooperating fully with the investigation and unable to comment further at this time", they added.


text:
Jolly Bus
The Jolly Bus was the colloquial term for buses that ran on the scheduled service operated by the W.H. Jolly Company, an independent bus company based in Sunderland, Tyne & Wear, England. The Jolly Bus would run from Sunderland town centre (map) to Evesham (map) and Claxheugh Road (map) in South Hylton.
The Jolly Bus "trademark" features were both the colour, cream with a brown stripe, and the rear seat, made of varnished wooden slats. W. H. Jolly also ran a small number of coaches.
Pressure from other services after dereugulation, coupled with the announcement of the Tyne & Wear Metro extension to Sunderland and South Hylton caused the company to close in 1995.
After the company closed the buses were sold to other companies. It is believed that KTY 23X was sold to Emsworth & District Motor Services before being donated to Asia Bus Response, as a response to the Tsunami Disaster of 26 December 2004. BGR 683W and BGR 684W were sold to Minsterley Motors and BGR 864W operated until at least 2006 as a school bus, when it was cited with safety issues[1] operating as a School Bus for Mary Webb School in Pontesbury. Mr Darren Parker Dix now owns the last remaining Jolly bus in England and is restoring it to original condition in the Sunderland area.
External links[edit]
- Minsterley Motors Bedford BGR684W
- Asia Response photographs
- South Hylton Local History Society, about W.H. Jolly
|This United Kingdom bus operating company article is a stub. You can help Wikipedia by expanding it.


text:
Latest HCV Stories
-SVR(12) rates of 96 percent were achieved in both SAPPHIRE-I (new to therapy) and SAPPHIRE-II (treatment-experienced with pegylated interferon and ribavirin) in adult patients with genotype 1 chronic
The European Association for the Study of the Liver (EASL) will this week be announcing new on-line recommendations on the management of hepatitis C (HCV) at the International Liver Congress™ (ILC).
NEW YORK, March 25, 2014 /PRNewswire/ -- At the New York Viral Hepatitis Summit presented by the National Black Leadership Commission
NORTH CHICAGO, Ill., March 24, 2014 /PRNewswire/ -- AbbVie (NYSE: ABBV) will present new data from its phase III hepatitis C development program at the 2014 International Liver Congress(TM) (ILC) in
Additional drug-drug interaction data for faldaprevir combined with commonly prescribed HIV medications also presented at CROI 2014 INGELHEIM, Germany and RIDGEFIELD, Conn., March
- Study Designed to Demonstrate Human Proof-of-Concept Results by YE 2014 - LA JOLLA, Calif., March 5, 2014 /PRNewswire/ -- Regulus Therapeutics Inc.
-- SVR(12) rates of 99 percent with and without ribavirin were achieved in genotype 1b patients new to treatment BOSTON, March 3, 2014 /PRNewswire/ -- The first detailed results
- Ninety-nine percent SVR(12) rates with and without ribavirin in certain patient types NORTH CHICAGO, Ill., Jan.
Researchers from Taiwan reveal that antiviral therapy for hepatitis C virus (HCV) improves kidney and cardiovascular outcomes for patients with diabetes.
Latest HCV Reference Libraries
Hepatitis C virus (HCV) is an enveloped, positive-sense single-stranded RNA virus of the family Flaviviridae. It consists of a core of genetic material surrounded by an icosahedra protective shell of protein. The genome consists of a single open reading frame that is 9600 nucleotide bases long. HCV replication takes several steps. It primarily replicates in the hepatocytes of the liver. It can also reproduce in peripheral blood mononuclear cells. There is a wide variety of genotypes and...
- Large; stout; burly.


text:
- Shopping Bag ( 0 items )
Posted March 18, 2005
If you give a child but one book this year let it be 'A Kick In The Head,' an eye-popping introduction to poetic forms. Now, don't be put off by the term 'poetic forms,' the examples win both young readers and adults. Who can resist Ogden Nash's 'In the world of mules, there are no rules.'? (A couplet, of course). Poet and teacher Paul B. Janeczko has included 29 poetic forms from haiku to a sonnet to an elegy. All are so thoughtfully chosen that one cannot suppress a smile or a catch in the throat. Among the authors represented are Shakespeare, Robert Service, Gary Soto, Georgia Heard, Richard Wilbur, the author himself, and, of course, everyone's favorite - anonymous. Among these pleasurable pages readers may learn why there are 17 syllables in a haiku, and 14 lines in a sonnet. Closing pages hold further notes on the various forms. An excellent suggestion from the author is to first read the poem, then read the explanatory note at the bottom of the page, next read the poem again to see if you can detect how it follows the stated form. Fairly bursting from the pages are Chris Raschka's watercolor, ink, and torn paper illustrations. Collage-like in appearance they capture the eye and couple perfectly with each poem. 'A Kick In the Head' is that rarity - a book to be enjoyed by both adults and children, and a joy to return to again and again. - Gail CookeWas this review helpful? Yes NoThank you for your feedback. Report this reviewThank you, this review has been flagged.


text:
Mick Mccarthy's Wolves side eased out of the relegation zone in the Premier League with a 3-1 win over West Brom today.
The vicory was a vital one for the former Irish manager as his side now has their fate in their own hands with two games left to play.
Wolves struck quickly with a goal in the 15th minute by Fletcher and were soon 2-0 up.
A goal right after half times seemed to seal an easy victory but West Brom hit back with a goal on 54 minutes and Wolves looked very nervous but managed to fight off their local rivals and ran out convincing victors in the end,
Once again it looks like McCarthy's men have pulled a Houdini and could well stay in the premiership.
They may well have key player Irish international Kevin Doyle back for the final two games as well.
Log in with your social accounts:
Or, log in with your IrishCentral account:
Don't have an account yet? Register now !
Join IrishCentral with your social accounts:
Already have an account ? Log in
For Newsletter Subscribers – Draw for 1 Prize on December 31st.
Prize: Your Piece of Ireland – a Square of Land in the heart of the Glens of Antrim, Ireland
More details here (or you can buy a little piece of Ireland directly): http://bit.ly/1zew9ox
Or, sign up for an IrishCentral account below:
Make sure we gathered the correct information from you
You already have an account on IrishCentral! Please confirm you're the owner.
Our new policy requires our users to save a first and last name. Please update your account:


text:
1956 Volkswagen Beetle Sedan Wall ArtItem # HN-ASR3973
- For indoor use only
- Transportation-themed wall art in beige
- Giclee print on canvas
- Vintage-inspired artwork
- Available in a variety of sizes
Similar Canvas Prints
KitchenAid KSM150PSGC 5 Qt. Artisan Series Stand Mixer - Gloss Cinnamon
Celestron AstroMaster 114 EQ Reflector Telescope
Bowflex SelectTech 1090 Adjustable Dumbbells- 10-90 lbs.
$279.99 - $829.98
Pearl Mantels Shenandoah Traditional Fireplace Mantel Shelf
$169.98 - $412.99
Precision Cape Cod Chicken Coop
Hayneedle Celestron AstroMaster 114 EQ Reflector Telescope
for reviewing this product when you join My Hayneedle Rewards
Write a review
200 points for each review = $2
300 points for reviews including an image or video = $3 Learn More
Customer Q & A
Enter your question, and one of our Customer Care experts will respond via email and also post the answer here.
Your Valid Email
Your Question


text:
7cm x 9cm Miniature Art done with graphite on drafting film, featuring a wonderful character who can be found drawing and painting away in a cave at The Rocks in the heart of Sydney. He kindly gave his blessings for me to take some photos of him while he worked, so I could use them as reference photos.
120 views as 19/02/10
Looks like a real character Karen and what a place to have a studio,lucky devil! Your piece does him great justice methinks.
Thank you so much Vic. I would rather have air conditioning, good lighting and a nice comfy chair, but I take my hat off to him and he certainly gets wonderful exposure.
– Karen Hull
Spent… a life well spent. It's the kind of concept that drives me mad. How do we decide the value in what we do?
The value in what we do is: expressing our creativity and sharing this creativity with others, thereby bringing them joy and inspiration and most important of all enjoying the process, so that you feel you are spending your life well!!!!
– Karen Hull
Karen ..this is wonderful..as usual…how kind of him to let you take photos..does he get many visitors to this intriguing studio ??
It is an open cave in an area that is extremely popular with visitors and tourists, especially on the weekends,, so I would imagine he gets a constant stream of visitors, which would drive me nuts, but he doesn't seem to mind.
– Karen Hull
As a well-spent day brings happy sleep, so a life well spent brings happy death. leonardo da vinci… beautiful Karen. It brought this quote to mind.
I LOVE this quote Bridie – thank you!!!!!
– Karen Hull
v nice my dear lovely
Thank you so very much Bobby!!! :)
– Karen Hull
Such a stunning & unique portrait, FAntastic & fabulous
Thank you so much for your kind and thoughtful words – much appreciated!!!
– Karen Hull
As always – beautifully captured by your outstanding talent Karen – simply wonderful!
Thank you so much Sharon – no closeup on the eyes this time, but I felt like doing something different xx
– Karen Hull
wonderful!!! :-)
Thank you so much Deidre!!!
– Karen Hull
This is fantastic Karen
Thank you so much Jacqui – I'm thrilled that you like it!!
– Karen Hull
Love it, a real slice of life work.
Thank you so much Jim – I always appreciate your comments!!
– Karen Hull


text:
- Fragrances
- Skincare
Add To Bag
Your item was successfully added to your shopping bag.
Showing 1 - 23 of 23 items
Back to the Armani roots, where it all began in 1984, Mr. Giorgio Armani created a fragrance that would stand the test of time and appeal to all generations beyond its current years.
Multiple Sizes
$66.00 - $98.00
The ultimate code of seduction.
Multiple Sizes
$45.00 - $93.00
Eau de Toilette
Multiple Sizes
$45.00 - $93.00
A masculine, contemporary fragrance that captures the essence of Giorgio Armani.
Multiple Sizes
$65.00 - $79.00
A soothing tonic that cools and comforts just-shaved skin.
3.4 oz
A light, distinguished EDT inspired by the fresh sea, warm sun and the richness of the earth.Giorgio Armani Fragrances will donate $5 to support UNICEF for each Acqua di Giò fragrance sold during March 1-31, 2015. For every Acqua di Giò limited edition purchased with PayPal until March 31st, 2015, PayPal will donate and additional $1 to UNICEF.
Multiple Sizes
$66.00 - $108.00
NEW. The scent of seduction for refreshing, on the go, all-day use.
This new classic is for all generations of men.
Multiple Sizes
$66.00 - $98.00
NEW Armani Code Ice is a fresher and more explosive variation of Armani Code.
2.5 fl oz
This luxurious balm pampers sensitive, just-shaved skin with soothing hydration.
3.4 oz.
Profumo by Giorgio Armani is the depth and intensity of the Mediterranean Sea.
Multiple Sizes
$95.00 - $130.00
This fragrant deodorant stick glides on easily for long-lasting protection.
2.6 oz.
NEW. A more intense and sensual interpretation of Acqua di Gio Homme.
Multiple Sizes
$80.00 - $172.00
Limited Edition 2.5 oz Eau Fraiche Spray
2.5 oz.
NEW. An intense, sophisticated and seductive fusion for the ultimate man.
Multiple Sizes
$70.00 - $88.00
Comforts and hydrates sensitive just-shaved skin.
3.4 oz
A sensual, woody fragrance inspired by contemporary masculinity.
Multiple Sizes
$64.00 - $78.00
This lightweight lotion moisturizes and soothes the skin after shaving.
3.4 oz.
A powerful deodorant stick for ultimate protection
2.6 oz
Giorgio Armani Fragrances will donate 100% of profits to support UNICEF for each Acqua for Life Acqua di Gio limited edition sold during March 1-31, 2015. For every Acqua di Giò limited edition purchased with PayPal until March 31st, 2015, PayPal will donate and additional $1 to UNICEF.
6.7 fl oz
A rich, luxurious shampoo that gently cleanses skin and hair.
6.7 oz.
The newest fragrance for men by Armani. This Woody Gourmand Aromatic is cut and polished like a diamond.
Multiple Sizes
$65.00 - $79.00
NEW. For a refreshing, on the go, all-day use.


text:
Pattern paper (for designing your own patterns)
Do fabric stores sell a type of paper for creating your own patterns? I saw a small package of something like it at JoAnns but the pieces were small so I didn't think that was what I'm looking for. I think I recall seeing pattern paper back when I used to sew clothes and was in fabric stores all the time (many moons ago!). Is there such a thing, and what do they call it so I can ask for it by name?
If not, what do you use to create your own pattern? For starters, I'd like to create just a square for a pillow top.
There are a number of papers you can buy for this purpose. Some come printed with a grid. These tend to be fairly expensive or, if you can find industrial suppliers, they come on a HUGE roll which will take you years and years to use.
I use a couple of products that Nancy's Notions sells; the prices are quite reasonable. The way the Nancy's Notions website is set up, I can't put in a link directly to the page. Just go to the website and put these item numbers in the search field:
Pattern paper, 21 inches by 77 yards. This is actually the same paper they use on examining tables in doctors' offices, if you have a source for that. Nancy's price is only $6.98 a roll.
This is stonger than tissue paper, you can see through it to trace things and it's easy to mark on it with pencil, pen or marker. I use it to trace the size I want from multi-sized patterns, then make any alterations on my copy.
Sewers Fix It tape. $2.98. This is also a medical product; it's paper adhesive tape. Use it to tape together sheets of the pattern paper, or to make alterations to your pattern. The big advantage to this tape is that you can run an iron over it and it doesn't melt because it is paper. It's exactly 1/2 inch wide, so you can use to to mark sewing lines on fabric, for instance, when you put in a zipper.
Also search for "pattern paper" to see some other pattern-making products.
For about $10 you can have everything you need to make your own patterns for a long time to come.
Here is a link that might be useful: Nancy's Notions
Fabric stores also carry a paper by the yard with little red dots all over it that used for pattern making.It's located with the interfacings.I believe walmart also sells thisI like this better than the examining table type stuff as it holds up much better and you can fold it roll it whatever.I just draw my lines on it with a ball point pen.
I use the sheets from an easel pad - pretty cheap at the office supply store, and a nice big piece of paper! You can get them with 1" grids, too.
I also have a LARGE roll of drafting paper I bought years ago at a drafting supply store. You probably can't find that anymore! I don't think any engineers or architects actually draw with paper & pencil anymore! :)
Newspapers, especially 'hometown type', sell ends of newsprint for a minimum price. It is about 24" wide and there are usually many yards on a roll. I have bought it for use with childrens activities, making patterns, and for packing when moving. It is clean with no print on it and is stronger than tissue paper. The last time I bought I paid $3.00 for a roll that was almost heavier than I could carry! Check it out!!
At college we have to use heavy brown paper, fine if you are going to store the patterns without folding them (in a big workroom) but a major pain if you have to carry the rolls of pattern back and forth on the bus! (just bought an expanding drawings tube to carry them)
I don't like this, because although the heavy paper lasts, it's brown - so you can't see through it for tracing! You are supposed to lay a thick cloth/felt on the table put down blank paper and then your original pattern on top. You then prick through it (with an awl) to mark out the shape of each piece. You end up with a blank sheet of paper covered in tiny holes that you then have to join up with a ruler. Nightmare!
I personally prefer proper tissue paper (Burda brand makes nice big sheets and is intended for patterns) which I can use to trace patterns in the more normal way. Like another poster above, I use this for copying multi-size patterns before making any alterations.
It is possible to buy Plotter Paper, white of 45gsm (the weight, normal letter paper is around 80 - 100gsm) I'd like to get this for my own use, but it's sold in 300metre rolls, so I have to be VERY sure, it's what I want, but it is quite cheap.
Hope you find what you want!
I've heard that the rolls of paper used in a doctor's office on the examining table work well if you have a friendly doctor you know from whom you could buy it. Otherwise, I bought a roll of plain white paper from Nancy's Notions (I think), and that works well, although I HATE tracing patterns!!!
I have a small role of Tyvek housewrap. It's perfect because one side is completely white which allows me to mark it and it's virtually indestructible. You can pin over and over again without destroying your pattern. The small rolls are only 36" wide and are available in home improvement stores; Lowes, Home Depot, etc.
You can get pattern paper for tracing patterns and making slopers at paragon patterns and also at Atlas Levy Sewing.
Here is a link that might be useful: Paragon Patterns
As a chronically starving artist, I'm always looking for ways to recycle things. I got the tail end of a roll of newsprint, years ago, from our local newspaper office. It's not ideal for patternmaking (tears easily, isn't see-through) but it was free and as I also do a lot of sketches (and like to let my little nieces and granddaughter draw, when they come over) it has been broadly quite useful. I've also used it, lots, for applique--I cut shapes out of it, pin pieces to it, iron the edges over it, sew the applique pieces down, about 7/8 of the way around, and then since it tears so easily, am able to take tweezers, reach under, and pull out all the paper, then sew down the remaining edge. For something also free, this is what I've been using for drafting smaller pieces of patterns (also good for my applique work): I buy those flat, large desk calenders, and when the months have expired (or if there are months I haven't used), I save the sheets and use them, back side up, to draw on. But I'm going to look into getting some Tyvek, for larger, durable pattern pieces. (You can see some of my applique work at my site--along with some of my jewelry designs, and thanks!)
Here is a link that might be useful: Heron Moon Designworks, on Etsy :)
I get mine at Clotilde.com she sells pattern paper and also swedish pattern material. You can cut out your pieces and pin or sew them together and try it on. I use it for patterns that I know I love and am going to use again and again.It does not tear or rip and folds easily for storage and you can write on it with marker.
Since my last post on this subject, I've started using Swedish Tracing Paper, 29 inches by 10 yards. It's pinable, sewable and really nice. Retails for $11.00 a roll at my local fabric shop. Distributed by Birch Street Clothing, Inc., www.birchstreetclothing.com, but probably available other places as well, like Nancy's Notions.
I also found the swedish tracing fabric scraps were very good to make the pages for a needle book. As my eyes have gotten older it is harder to pick out a good needle from the pin cushion, so I made one of these needle books and it is easier to pick out the one I want to use and it keeps them out of the way when I am working. I never was too good at using a pincushion.
If you just need to draft a few pieces, you can use an old sewing pattern (Yikes ~ I don't mean collectible or vintage!) which is incomplete or one you won't ever use if it has uncut tissue big enough for what you want to draft. Just make sure it's not a special pattern that you'd be cutting up!
how do i use pattern paper???! do i like trace the shape of the article of clothing im making with using my measurment and then lke sew the whole thing together and when its done i just pull the paper off???? is that right? i need to learn how to sew a bikini within the next 6 weeks!!! where do i get the liners?? :*
I like this topic. A local art supply store is my biggest pattern altering/creating friend. I use multiple bright pastel pencils to keep steps separated. I use roles of tracing paper when I can get them on sale. For mock-ups or trials I use (non-quilters) grid paper, or use old king size flat sheets. This last trip I bought 100% recycled newsprint. It does require serious underneath to trace, but with the pastels I can trace the pattern lines(on the back of tissue to prevent mirror image issues), lay the newsprint on top and rub firmly to transfer. Then you use your marker of choice and darken it up.
It's been a long time since I've worked in a fabric store, but I remember something called Pattern Ease. It was on a bolt with the interfacings, looked like white sew-in interfacing but it was stiffer, I think. I would think sew-in interfacing would do just as good.


text:
Mayo Clinic Reports 2014 Was Strong Financial Year
Rochester, MN ( KROC AM News) - Mayo Clinic has released its 2014 financial results and they show a fairly strong performance.
Rochester, MN ( KROC AM News) - Mayo Clinic has released its 2014 financial results and they show a fairly strong performance.
Rochester, MN (KROC-AM News) - It looks like Rochester will join other cities around the state in getting rid of its pet licensing program.
Nasty travel conditions are being forecast for sections of southern and western Minnesota on Tuesday.
Rochester, MN (KROC AM News) A Rochester man and his girlfriend were shaken up but not hurt early Monday morning when a bullet struck the vehicle they were in.
Rochester, MN ( KROC AM News) - Gasoline prices have shot up in Rochester, something that has been reported around the country in recent days.
A worker at a Rochester construction site was killed Friday.
A child pornography case involving a Rochester man has been taken over by federal prosecutors.
The Rochester School Board will be presented Tuesday with the results of a recent survey that was commissioned to gauge the level of support for a possible referendum in November.
It was very cold across southeast Minnesota Friday morning as overnight temperatures fell below zero.
The Destination Medical Center Corporation Board spent most of its meeting Thursday discussing the transportation portion of the proposed plan.


text: http://serverfault.com – I have a huge mysql database , say about 15G size . Now I want to move the database to another mysql server , the normal way to do this is to export mysql file then import this file to the target server . But I find it is so slow and error occured in this process . Any better way to do this ? Please let me know . Thanks . (HowTos)


text:
In the process of buying a house and we have found out the pool was built in 1969. We have not had a chance to have the pool inspected yet because of the snow/weather. I am curious to know if there is an average lifespan to inground pools?
- Forum >
- Miscellaneous >
- inground pool question
Just my opinion, but a pool of that age must have some problems. Wait until you sign anything so you can get an independent inspection of the pool. The previous homeowners will not tell you diddly. Inground pools require much more maintenance then above-ground pools. Chlorine takes it's toll on pumping components and rubber O rings. As far as the concrete goes, it may be fine if it was a good pour and sealed and weatherized each season.
I agree with PlumberTom. I wouldn't buy a house that I wasn't able to inspect all components. That could get quite costly to fix.
Good luck,
If you are hellbent on buying the house without inspecting the pool maybe you could do some private investigating and call local pool companies to see if the pool has ever had problems. I don't know if they could tell you that or not.
I have to vote against buying the house during the winter.
Your real estate agent isn't offering you any advice? Maybe try the real estate section.
Good luck,
sod removal equiptment
Grounding a swimming pool
pool deck and retaining wall
how to find leak in swimming pool pipe
- 15 Old House Features We Shouldn't Abandon
- 17 Tiny Bathrooms We Love
- 16 Designs for a Low-Cost DIY Coffee Table
- Insanely Easy 60-Minute Home Improvements
- 12 Sheds You Could Live (or Work) In
- Assembly Required: 15 DIY Kit Homes
- 30 Things Every Adult Should Know How to Do
- 10 Surprisingly Simple Woodworking Projects
- 7 Surprising Other Uses for Mayonnaise
- 9 Ways to Make Your TV Look at Home
- 9 Totally Amazing Mobile Home Makeovers
- 11 Lessons to Learn from AirBnB's Tiniest Homes
- 10 DIY Ways to Redo Your Wall—Without Paint
- 8 Smart Shoe Racks You Can Make Today
- 7 Easy Budget-Friendly Backyard Makeovers
- Worth It: 8 Renovations That Pay You Back
- 7 House Sounds Never to Ignore
- Watch These 10 Home Trends Take Off in 2015
- 11 Things Never to Keep in Your Bedroom
- 12 Places You Never Clean—But Should!


text:
Winter Roll Call in 9b
Here is what did well over the winter and what did not in my 9b yard. My frost cloth covering was spotty as I was out of town and left instructions for teenage son to take care of yard.
Super Troopers (things that are supposed to be cold sensitive but had no damage)
Lychee Sweetheart Tree
Dwarf Jasmine Tree
Ruby Glo Passionflower and Lunametista
Nun's Orchids
Australian Tree Fern
New Guinea Trumpet Creeper
Pinstripe Ginger
Wild coffee plant
Hoya Carnosa
Jungle Drums Ginger
Nam Doc Mai (in most protected part of yard, which is a good microclimate) I was horrified to learn out of all the plants on the coldest night, he forgot to cover this tree. On nights I was at home I covered it the rest of the time. No damage at all.
Minor damage or comes back readily
Night Blooming Jasmine
Red Absynnian Banana, Milky Way, Ruby Siam, and Rajanpuri Banana
Hawaiin Sunset Vine (lost leaves for 2 weeks, regrowing)
Naranjilla (very minor leaf damage)
Sugar Apple Tree
Jacaranda Tree
Malaysian Ginger
Midnight Ginger
Dwarf Poinciana Trees
All tropical water lilies regrowing after dormancy without protection
R.I. P. or may never be the same
Panama Red Passion Flower Vine (lost all leaves and 2/3 of plant)
Large Leaf Princess Plant
Dwarf Ylang Ylang would have been OK if my son remembered to cover it,
Is regrowing from bottom, lost all leaves and about half of plant.
Things that are growing in pots on the lanai without problem: Royal Poinciana, Black Bat Plant, Pony Tail Palm, Pickering Mango and variegated Pitch Apple.
Charlie Brown's Football (things I try over and over but fail)
Mandevilla (looks straggly and near death no matter what I do)
Butterfly Bush (see above)
New Zealand Tea Tree
This post was edited by morningloree on Mon, Mar 3, 14 at 19:26
The best hummingbird attractor I have is my Dwarf Poinciana trees. They are deciduous in my zone. I have one outside my kitchen window and it attracts so many pollinators: butterflies, hummingbirds, hummingbird moths, and bumblebees. It doesn't get flowers as large as the royal poinciana, but they are pretty none the less. I also have a Blue Vitex that I have seen one around and Red Firespike.
Hi Dawn
Thanks for the informative list...this is good knowledge for me since we are nearby.
I was surprised that these plants did well with frost protection that was spotty (perhaps the frost was spotty, too):
Plumeria (I've been watching several in my neighborhood that were totally neglected in the cold)
Plants Left To Their Own Devices
No protection for my Purple Firespike and blooming its head off through all the winter chills
same for my begonias - weird, huh?
My poor clerodendun minahassae lost all its foliage, but I see new buds popping out now.
same for my sanchezia
I can't tell yet, but I think the bananas will be alright.
And my tibuchinias under the orange tree only lost some leaves when we had a lot of wind
And, of course, none of my roses even skipped a beat during the cold (which is why keep growing my collection)
For some reason, my Dwarf Poincianna kept its leaves and even a flower or two. But it's little and still in a pot close to the carport.
> Posted by susieqsie... Mar 5, 14
> Purple Firespike... blooming its head off
Susie, is it the same plant, about which you wrote in a different thread,
that had started blooming in September?
You questioned : "maybe it's the one they call magenta".
What are your thoughts about that now?
Oh, and is the September (year ago) one
doing it again this fall?
Hi Four
My firespikes are blooming now, the purple and the red, but they look pathetic.
I think the purple just started blooming and the red blooms are leftover.
The one that looked so awesome last Sept was in Mom/Dad's yard. They thought it was too tall for its location and whacked it back. I'll take a look at it on Turkey Day and see how it is faring.
I think there is too much shade for my plants this year. There's some icky black stuff on their leaves (sooty mold, maybe) that is all over everything in these woods behind my house. That's probably why the blooms look so pitiful.
If we ever get a day without cloudbursts, I'll take some pics so you can see.


text: http://askubuntu.com – I was using Natty for a while, but while updating to the new release there was a blackout and it wasn't able to finish and Ubuntu failed to load after that. I thought, no worries I got my files backed up and I still got my 10.10 CD I used to put Ubuntu in my computer in the first place. So I installed it again, with the plan of using the update manager to get myself with the current release... Except I get this error: W:Failed to fetch http://extras.ubuntu.com/ubuntu/dists/natty/main/source/Sources.gz 404 Not Found , W:Failed to fetch http://extras.ubuntu.com/ubuntu/dists/natty/main/binary-a (HowTos)


text:
I have frequented this restaurant on many occasions but have never reviewed it. It was a spur of the moment decision to visit and I was not disappointed. I had the BBQ Chicken Sandwich on garlic bread with baked beans. I add the salad bar to my order as well. The chicken was so tender and juicy, garlic bread toasted just enough, baked beans sweet and what can I say about the salad bar - lots and lots of items.
If you own or manage Sonny's BBQ, register now for free tools to enhance your listing, attract new reviews, and respond to reviewers.


text:
Click to Win!
Wednesday, April 1, 2015
Photo of the Day
Events Calendar
Taste of Soul
Buy Cookbook
Crenshaw & Around
Breaking News
Celebration of Life
Campaign Watch
Press Releases
Argentine Jorge Bergoglio elected Pope Francis
Written by Nicole Winfield, Associated Press
13 March 2013
Pope Francis speaks from the central balcony of St. Peter's Basilica at the Vatican, Wednesday, March 13, 2013. Cardinal Jorge Bergoglio who chose the name of Francis, is the 266th pontiff of the Roman ...
read more >>
Bodies exhumed in killings tied to Mandela ex-wife
Written by Michelle Faul, Associated Press
12 March 2013
Nomsa Tshabalala, second from left, with relatives surrounding the presumed grave of her son Siboniso Tshabalala, at Avalon Cemetery in Johannesburg, South Africa, on Tuesday, March 12, 2013. Forensic ...
read more >>
Recent heat spike unlike anything in 11,000 years
Written by Seth Borenstein, Associated Press
08 March 2013
(Photo: Associated Press) WASHINGTON (AP) — A new study looking at 11,000 years of climate temperatures shows the world in the middle of a dramatic U-turn, lurching from near-record cooling ...
read more >>
Dennis Rodman worms his way into North Korea
Written by Associated Press
07 March 2013
North Korean leader Kim Jong Un, left, and former NBA star Dennis Rodman watch North Korean and U.S. players in an exhibition basketball game at an arena in Pyongyang, North Korea, Thursday, Feb. 28, ...
read more >>
More Than $14M of Fake HandbagsSeized by CBP in L.A.
Written by Sentinel News Service
05 March 2013
Office of Public AffairsU.S. Department of Homeland Security March 5, 2013Contact: CBP Public Affairs, Jaime Ruiz(562) 980-3110 NEWS RELEASE More Than $14M ...
read more >>
Kenya prepares for watershed elections
Written by Associated Press
28 February 2013
Associated Press/Khalil Senosi, In this Feb. 24, 2013 file photo, Orange Democratic Movement (ODM) Presidential candidate Raila Odinga, right, shakes hands with The National Alliance (TNA) Presidential ...
read more >>
Despite peace accord, eastern Congo still on edge
Written by MELANIE GOUBY Associated Press
25 February 2013
Associated Press/ Elias Asmare - Joseph Kabila , President of the Democratic Republic of Congo, waits for the signing of the Congo peace agreement, in Addis Ababa, Ethiopia, Sunday, Feb. 24, 2013. Despite ...
read more >>
Victims of Kenya's last election still in limbo
Written by TOM ODULA Associated Press
25 February 2013
In this photo of Saturday Feb. 9, 2013, a displaced woman works with others to construct a new straw hut at 'Hope' camp for internally-displaced Kenyans from the Kikuyu tribe (Ben Curtis Associated ...
read more >>
Minnesota congressman arrives in Mogadishu
Written by ABDI GULED and JASON STRAZIUSO Associated Press
20 February 2013
Somali President Hassan Sheikh Mohamud, left, and US Congressman Keith Ellison give a joint press conference at Mogadishu airport, Tuesday Feb. 19, 2013. Ellison said Tuesday that his visit to Mogadishu ...
read more >>
NKorean propaganda video shows Obama in flames
Written by Associated Press
20 February 2013
In this Dec. 10, 2010 file photo, President Barack Obama gestures as he speaks to workers about the economy during a visit to Daimler Detroit Diesel in Redford, Mich. A new North Korean video portrays ...
read more >>
S.Africa police: Congo rebel 'ringleader' arrested
Written by JON GAMBRELL Associated Press
11 February 2013
Unidentified suspects are led out of court after appearing in Pretoria, South Africa, Thursday, Feb. 7, 2013. Nineteen alleged members of a Congolese rebel group including one U.S. citizen sought help ...
read more >>
Indonesia readies for $1 trillion trade talks
Written by JOHN HEILPRIN Associated Press
01 February 2013
United States Trade Representative, Ron Kirk, gestures as he speaks during an interview with The Associated Press at the World Economic Forum, WEF, in Davos, Switzerland, Saturday, Jan. 26, 2013. ...
read more >>
Arabs, Tuaregs in Mali face hostility amid war
Written by KRISTA LARSON | Associated Press
01 February 2013
In this photo taken Saturday Jan. 26, 2013 Bawba Mint Baba Ahmed is photographed in Mopti, Mali. Bawba Mint Baba Ahmed's sewing teacher singled her out in front of the class, telling her: "You look ...
read more >>
Kenya elections observers to enhance transparency
Written by By TOM ODULA Associated Press
28 January 2013
In this Thursday, Jan. 3, 2008 file photo, a young girl cries as she is carried by a man and woman fleeing an area of wooden kiosks which was set on fire by supporters of Raila Odinga's party, the ...
read more >>
Mali military enters fabled town of Timbuktu
Written by KRISTA LARSON and LORI HINNANT Associated Press
28 January 2013
Chadian soldiers patrol the streets of Gao, Northern Mali, Monday Jan. 28, 2013. Malian soldiers descended on the city of Timbuktu on Monday after al-Qaida-linked militants fled into the desert having ...
read more >>
Angry protests, clashes on Egypt anniversary
Written by HAMZA HENDAWI and LEE KEATH | Associated Press
25 January 2013
Egyptian protesters take cover as they clash with riot police, not seen, near Tahrir Square, Cairo, Egypt, Friday, Jan. 25, 2013. Two years after Egypt's revolution began, the country's schism was ...
read more >>
Algeria: 12 hostages have died in the siege
Written by Anis Belghoul and Paul Schemm Associated Press
18 January 2013
This is a April 19, 2005 fiel photo released by Statoil via NTB scanpix, shows the Ain Amenas gas field in Algeria, where Islamist militants raided and took hostages Wednesday Jan. 16, 2013. British ...
read more >>
Palestinians: Apartheid state if Netanyahu wins
Written by MOHAMMED DARAGHMEH Associated Press
18 January 2013
In this photograph made on Sunday, Jan. 13, 2013, print house employees work on an election campaign billboard of Israeli Prime Minister and Likud Party leader Benjamin Netanyahu, in the central Israeli ...
read more >>
Haiti quietly marks quake's 3rd anniversary
Written by TRENTON DANIEL Associated Press
18 January 2013
Relatives of those who died in the 2010 earthquake walk in single file to place a cross on a hilltop to remember those who died in the devastating earthquake, prior to a memorial service at Titanyen, ...
read more >>
Ivory Coast returns stampede bodies to families
Written by Associated Press
12 January 2013
A young relative weeps during a memorial service for the 64 people killed in a New Year's stampede, as family members came to claim the bodies of their loved ones at Treichville's University Hospital ...
read more >>
France vows help after Mali Islamists take town
Written by BABA AHMED and KRISTA LARSON Associated Press
12 January 2013
In this Aug. 31, 2012 file photo, fighters from Islamist group Ansar Dine stand guard in Timbuktu, Mali, as they prepare to publicly lash a member of the Islamic Police found guilty of adultery. The ...
read more >>
Finding the salt of the soil of Benin
Written by Sunday Alamba Associated Press
12 January 2013
Kakpo Augustine, left, and Victorine Accrombessi, right, dig up soil in the village of Djegbadji near Ouidah, Benin, on Friday, Jan. 11, 2013. Artisanal salt farmers in this village in Benin dig off ...
read more >>
UN welcomes peace deal in Central African Republic
Written by Associated Press
12 January 2013
President Francois Bozize AP photo The U.N. Security Council is welcoming the cease-fire agreement and peace deal in the Central African Republic that will allow President Francois ...
read more >>
Ivory Coast New Year's death toll rises to 64
Written by Associated Press
05 January 2013
Flowers are left by a sign reading "to our victims" in Abidjan, Ivory Coast, Wednesday Jan. 2, 2013. Survivors of a stampede in Ivory Coast that killed 61 people, most of them children and teenagers, ...
read more >>
New child soldier fears in C. African Republic
Written by Associated Press
05 January 2013
A youth dances in the rap band entertaining a meeting of the National Youth Council to discuss the current crisis and the message that youth want to send to upcoming peace talks in Libreville, at ...
read more >>
Page 4 of 9
WIN TICKETS to see Alvin Ailey American Dance Theatre April 15th - 19th at the Dorothy Chandler Pavilion!
LASTV and More!
Wendell James interview at the 46th Annual NAACP Image Awards
Watch Video >>
Film Director Princess Monique interview at the 46th Annual NAACP Image Awards
Watch Video >>
Garcelle Beauvais interview at the 46th Annual NAACP Image Awards
Watch Video >>
Carlos King interview at the 46th Annual NAACP Image Awards
Watch Video >>
Judge Greg Mathis interview at the 46th Annual NAACP Image Awards
Watch Video >>
Taste of Soul Sponsors
Click to
Events Calendar
Sat Apr 4 12:00AM
Saponi Star Productions Presents ER: The Resurrection Stage Play
Sun Apr 5 12:00AM
Saponi Star Productions Presents ER: The Resurrection Stage Play
Thu Apr 9 6:00PM
Charles R. Drew University of Medicine and Science Legacy Leaders Spring Gala
Sat Apr 11 11:00AM
Books! Books! Book! for all ages
Sun Apr 12 11:00AM
Berean's Women's Ministry Presents: Etiquette Classes
Sat Apr 18 9:00AM
Black Writers On Tour & Southern California Black Business Expo
Sat Apr 25 2:00PM
2015 "One Child At A Time" Celebrity Flag Football Game
View Full Calendar
Subscribe Now!
Local »
Man Indicted for Teacher's Stabbing Death
LMU AAAA Awards Scholars
Report: Minimum Wage Hike Would Have More Benefits Than Drawbacks
Long Beach Murder Reveals Ongoing Gang Dispute
National »
US Files Appeal of Judge's Hold on Immigration Action
Feds Charge U. of Mississippi Student in Noose Incident
4 Journalists Arrested During Ferguson Protests Sue Police
Obama announces 0M in new pledges for STEM education
International »
Obama: Dim hope for end to Israeli-Palestinian conflict
International Court: UN must help with the arrest of Sudan's leader
Supreme Court declines to hear ex-Somali official's case
Italy rescues more than 1,000 migrants at sea; 10 perish
Business »
20th ANNUAL COMMUNITY JOB FAIR AT DODGER STADIUM SATURDAY
Local Real Estate Broker Attends National Apartment Association Conference in Washington D.C. S
Chick-Fil-A on South Figueroa St. Reopens After Fire
Multicultural Consumers Transforming the U.S. Mainstream
Entertainment »
Legendary singer/songwriter Andrea Martin is back with mixtape Nothing New
Kerry Washington signs on to play Anita Hill in HBO Movie Confirmation
From Music Mogul to Sheriff: Terrence Howard Stars in FOX's Wayward Pines
Kevin Hart to receive "Cinemacon Comedy Star of the Year Award"
Sports »
Stanley Johnson Helps Arizona Cancel Xavier! No. 2 Seed Wildcats will play No. 1 Badgers in Elite 8
LAKERS WATCH : Wisconsin Beat North Carolina 79-72
Staples to Host NCAA West Regional
Profile: Lawrence Taylor Walker Taking His Game beyond The Court
Religion »
Bishop Vaughn shatters glass ceiling – AGAIN!
AME Church launches 'Black Friday' campaign
Transfiguration students excel in many areas
Crowd funding is NOT new to the Church!
Family »
'My Sister's Keeper' Launched at Howard University & Spelman College
CSUDH Professor Receives Fulbright Award
Black Employee Files Wrongful Termination Suit against Mount St. Mary's
Dr. Robert R. Cole Succumbs
Crenshaw & Around »
Kaiser Permanente Hosts Basketball Clinic For Special Olympics Athletes
APLA Health & Wellness Chief Operating Officer Vallerie Wagner to Receive Advocate Award
Autism Experts, Elected Officials Aim To Address The Need In Underserved Communities
New Changes to Medicare and Medi-Cal Impact South L.A. Seniors
Opinion »
"Empire" Shows that Black Dollars Matter
March Madness: Race, Sports and Money
U.S. Criminal Justice System Needs Urgent Reform
I Had a Heart Attack
Editorials »
Letter from the Co-Editors
This Has to Stop!
SB 405 & AB 158: Bills that kill jobs and harm the environment
"I've Been Looking for Senator Obama All My Life"
Blog »
Season of Giving
Culver City Police
Sports and Life
2009...2010...It's time!
Sentinel Partners
Taste of Soul
LA Watts Times
The Los Angeles Sentinel
Customer Care
Contact Us
Media Kit
Corrections / Misprints
Privacy Policy
Scroll To Top
2015 ©
The Los Angeles Sentinel
All rights reserved.


text:
Painting over new texture
Has anyone ever painted new construction drywall with orange peel texture and have their paint coatings peel off in small sheets.
This was my process....
Used top quality eggshell finish as base coat since the inexpensive PVA drywall primer is nothing more than a inexpensive flat paint. I felt I was using a better paint as primer coat with better acryics than cheaper sister product as PVA primer. I placed two coats on wall. In numerous areas, my coatings is peeling off once surface film is broken.
Checked texture and found if I rubbed withmy two fingers lightly, the texture turned to dust like it was sanded. I then wet my fingers and it quickly turn to a white powerded paste.
In certain areas, it peel the texture down to the drywall itself.
I throughly cleaned an area that had peeled off and used a high quality primer. Waited 4hrs to dry. Placed blue tape over area and primer peeled off.
This is leading me to believe that the texture was mixed wrong or something. I firmly believe in the system used but looking for addition direction with experienced examples.
Hello "orange-peel" fans!!!
>>> Sorry Darren-painter...
* The 1st mistake was using a "paint" where it shouldn't be...right on chalky-texture & bare drywall paper!!
* PVA (poly-vinyl-acetate) primer should've been used. IDEALLY b4 AND after spraying texture. Not many painter do, but it's the "ideal" way.
* At a minimum, priming HAS to be done after texture is dry.
* Primers & paint are 2 different things. A decent primer will seal-off the VERY POROUS drywall paper & the sprayed texture. Paints will just largely soak-in...and unevenly at that!
>>> Your texture did what it would naturally do...crumble & get pasty when wet! It's just drywall-mud & water sprayed on wall.
* When this mix dries, it's basically "chalk-lumps"! What happens when you sand drywall joints?? Same thing...lots of dust!
* Your "paint film" will peel off these texture-lumps VERY easily, since they're BARELY adhered to the drywall paper. A paint film applied to this will start to form a skin, and easily peel off the chalky texture.
* If wall had been PRIMED b4 texturing, it has a better "grip" to the primer than to bare drywall paper.
>>> Your primer was peeled off by the tape because it takes time to form a tough-enough bond to wall. Primers are made to seal, not be tested by tape!!
* You need to slit the primer/paint film with a knife at the tape-edge when done painting.
* The film that bridges onto the TAPE, is stronger than the bond to the primed wall...FOR MANY HOURS after paintings' done!!
* This "bond-forming" takes DAYS. Painted walls aren't considered CURED/hardened-off for a MONTH.
* I purposely wait till next day to slice at the tape-edge! That way it's a nice dry cut.
>>> To summarize, your texture-situation is completely normal.
>>> Up here, I grew up seeing "orange-peel" everywhere. I still think it's odd seeing SMOOTH walls! I don't care for smooth...too annoying seeing little flaws/waves.
>>> You'll also have to get used to using REAL primer, and TWO topcoats of paint. THAT'S what produces the best finishes.
Have a good one!


text:
Yes! Evenlyn PutterThe Evelyn-12 is a Tour-inspired mallet shape that has large weight pads heel and toe for a high MOI for its relative head size making it incredibly stable. The black PVD body also contrasts with the white True-Square alignment lines to make alignment automatic whether you are right- or left-eye dominant.
Fitting Recommendation
C-Groove Technology
Every face is perfect because it's 100% precision CNC milled with no inconsistencies and the highest tolerances. The 20-degree upward cut of the C-Groove's concentric edges grip the ball's surface and apply physical forces that simultaneously lift the ball out of its resting position. Yes putters achieve forward roll 21.5% quicker than other putters which repeatedly skid, skip and spin before settling into forward roll.
Vibration Dampening Insert
A precision-milled "through-slot" located behind the putter's face holds a thermoplastic polyurethane insert (TPU 65D) that captures unwanted vibration for exceptional feel and responsiveness.
True-Square Alignment
The TPU insert, cavity alignment line and the center of the C-Grooves on the face align perfectly together on a single plane. This is True-Square Alignment and will help you frame the ball closer to the center of gravity location. Whether you are left eye or right eye dominant, True-Square helps you square the putter to the ball with the alignment lines on the topline and cavity.
Recent Products
Featured Products
TaylorMade R15 DriverPrice: $429.95
Arccos Golf GPS & Stat Tracking SystemPrice: $399.95
Odyssey Works Tank Versa 2 Ball Fang PutterPrice: $219.95
Callaway XR DriverPrice: $349.95
Callaway 300 Laser Golf RangefinderPrice: $279.95
Ecco Freddie Couples Limited Edition Masters - Casual Hybrid Golf ShoesPrice: $199.95
Garmin Approach S6 GPS Golf Watch - WhitePrice: $399.99
Mizuno JPX-850 Iron SetPrice: $799.95
Game Golf - Digital Tracking SystemPrice: $199.00
2015 Sun Mountain MC3 Micro Golf Push CartPrice: $199.95
TaylorMade AeroBurner Mini DriverPrice: $279.95
2015 Sun Mountain H2NO Lite Golf Cart BagPrice: $299.95


text:
Local artist, Aaron Kizer, gets national recognition with his amazing Steve Jobs tribute painting, created in just minutes before your eyes.
Upcoming Events
Event Finder
Restaurant Finder
The Weekender
Subscribe to The Weekender, Louisville's weekly guide for where to go and what to do!
Most Read Stories
- Ear X-tacy Graffiti Appears on Bardstown Road Panera
- 2013 Louisville Area Child Support Arrears List (Deadbeat Parents)
- Louisville Long-Term Bucket List
- Where to Go and What to Do in Louisville This Weekend (March 26 – 29)
- Little Free Libraries Around Louisville
- GonzoFest Louisville Raises Funds for Hunter S. Thompson Statue
- 15,000 people are headed to Louisville for the VEX Robotics Championship
- Review: Storm Large Wows The Mercury Ballroom
- Start Planning for Thunder Over Louisville 2015
- Power Singer Storm Large and Louisville Orchestra's Teddy Abrams play Mercury Ballroom March 24