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Synthesis and Cerebral Uptake of 1-(1-[11C]Methyl-1H-pyrrol-2-yl)-2-phenyl-2-
(1-pyrrolidinyl)ethanone, a Novel Tracer for Positron Emission Tomography Studies of
Monoamine Oxidase Type A
Svend Borup Jensen,*,†Roberto Di Santo,*,‡Aage Kristian Olsen,†Kasper Pedersen,†Roberta Costi,‡Roberto Cirilli,§and
Paul Cumming†
PET Centre, Aarhus UniVersity Hospital, Nørrebrogade 44, 8000 Århus C, Denmark, Istituto PasteursFondazione Cenci Bolognetti,
Dipartimento di Studi Farmaceutici, UniVersità di Roma "La Sapienza", P. le A. Moro 5, I-00185 Roma, Italy, and Dipartimento del Farmaco,
Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy
ReceiVed August 13, 2007
(R)-(-)- and (S)-(+)-1-(1-[11C]methyl-1H-pyrrol-2-yl)-2-phenyl-2-(1-pyrrolidinyl)ethanone 4 and 5 were
synthesized, and their properties as tracers for positron emission tomography (PET) studies of monoamine
oxidase type A (MAO-A) in the brain of living pigs were tested. Parametric maps of the distribution volume
(Vd) 4 in pig brain were qualitatively similar to those obtained with [11C]harmine, with prominent binding
in the ventral forebrain and mesencephalon. Its binding was highly vulnerable to MAO blockade, suggesting
a binding potential as high as 2 for MAO-A sites. The slow plasma metabolism of 4 and 5 may present
advantages over [11C]harmine for routine PET studies of MAO-A.
Monoamine oxidase (MAO, EC 18.104.22.168) types A and B are
flavin-containing enzymes that contribute importantly to the
catabolism of dopamine and other biogenic primary monoamines
in the brain.1The discovery of the antidepressant property of
the irreversible MAO-inhibitor N′-propan-2-ylpyridine-4-car-
bohydrazide (iproniazid) was an early breakthrough in psy-
chopharmacology.2However, there have been relatively few
ligands suitable for studies of MAO by positron emission
tomography (PET). The propargylamine-compound [11C N-me-
amine ([11C]clorgyline) is a suicide MAO substrate with
considerable selectivity for MAO-A,3,4and [11C N-methyl]N-
methyl-N-(1-methyl-2-phenyl-ethyl)-prop-2-yn-1-amine ([11C]de-
prenyl) is an irreversible inhibitor of MAO-B in living brain.5,6
However, the kinetic analysis of the cerebral binding of these
propargylamine ligands is hampered by the very high net
clearance from brain and rapid irreversible binding to MAOs,
such that net clearance from the brain approaches the limit
imposed by cerebral perfusion. Deuterium substitution of the
R-carbon of [11C]deprenyl results in a slower rate of association
with the MAO-B, which is more favorable for kinetic analysis.7,8
However, for reasons that remain uncertain, the deuterated
derivative of [11C]clorgyline yields cerebral-binding maps
endowed with relatively poorer contrast between regions of low
and high MAO-A activity than those produced by [11C]clor-
gyline itself.8,9Several clorgyline analogues synthesized for PET
or SPECT (11C-fluoroclorgyline,10 18F-fluroclorgyline,11,12and
[125I]iodoclorgyline) showed low cerebral uptake.13
One of the best-characterized reversible PET ligands for
imaging MAO-A is [11C-MeO]7-MeO-1-Me-9H-pyrido[3,4-b]-
indole ([11C]harmine, a ?-carboline alkaloid, which has been
employed in both preclinical studies on animals14–16and a
clinical study of depression in humans).17This tracer is very
rapidly metabolized in peripheral tissues (only 10% of the
plasma radioactivity remains as untransformed [11C]harmine at
10 min), which can be an encumbrance to the quantification of
its cerebral binding. Another MAO-A PET ligand, [11C-1](5R)-
y]phenyl]-2-oxazolidinone ([11C]befloxatone), is characterized
by relatively slower peripheral metabolism.18,19However,
[11C]befloxatone is synthesized via [11C]phosgene, a dangerous
reagent available only at a few PET centers.
The lack of a ideal ligand and the emerging importance of
MAO-A in PET studies of depression17and tobacco addiction20,21
justify the continued search for a ligand with optimal properties.
Recently, we designed and synthesized a series of pyrrolyle-
thanonamines (general structure 1; Chart 1) that showed potent
activities as MAO inhibitors, as well as high selectivity against
* To whom correspondence should be addressed. Telephone: +45-8949-
3034. Fax: +45-8949-3020. E-mail: email@example.com (S.B.J.); Telephone/
Fax: +39-6-49913150. E-mail: email@example.com (R.D.S.).
†Aarhus University Hospital.
‡Università di Roma "La Sapienza".
§Istituto Superiore di Sanità.
aAbbreviations: Vd, distribution volume; t0, dead time of the column;
R, enantioselectivity; CSP, chiral stationary phase; Ca(T), arterial input
function; PR(t), total plasma radioactivity; PPR(t), plasma precipitate
radioactivity; ftracer, unmetabolized parent radioligand.
Chart 1. Pyrrolyletanoneamines 1-3 Endowed with Potent
Activities against MAOs and Newly Designed PET Tracers 4
and 5a
aThe position with the asterisk shows that the ligands have been labeled
with carbon-11.
J. Med. Chem. 2008, 51, 1617–1622
10.1021/jm701378e CCC: $40.75
2008 American Chemical Society
Published on Web 02/29/2008
Page 2
MAO type A. This selectivity was demonstrated against the two
specific isoforms on MAO in a mitochondrial preparation from
bovine brain, according to the method of Basford.22Within this
series of MAO inhibitors, we identified (R)-(-)-1-(1-methyl-
1H-pyrrol-2-yl)-2-phenyl-2-(1-pyrrolidinyl)ethanone (R-RO-
MAO, 2) as a candidate for a PET study for MAO-A based on
its 200 000-fold selectivity for MAO-A over the B form,23
exceeding even the 52 000-fold selectivity found of the S
enantiomer (S-ROMAO, 3). Furthermore, the chemical structure
of these compounds is amenable for [11C]-labeling (Chart 1).
Our strategy to obtain the compounds (R)-(-)-1-(1-[11C]methyl-
1H-pyrrol-2-yl)-2-phenyl-2-(1-pyrrolidinyl)ethanone (R-[11C]RO-
MAO, 4) and (S)-(+)-1-(1-[11C]methyl-1H-pyrrol-2-yl)-2-
phenyl-2-(1-pyrrolidinyl)ethanone (S-[11C]ROMAO, 5) (Chart
1) was based on the synthesis of (R)-(-)- and (S)-(+)-2-phenyl-
1-(1H-pyrrol-2-yl)-2-(1-pyrrolidinyl)ethanone (6 and 7), which
underwent a [11C]-methylation on position 1 of the pyrrole ring
to afford the required labeled derivatives 4 and 5.
We have obtained more than a decade of experience with
the pig as an animal for preclinical investigations of radioligands
targeted at neurotransmitter systems.24–26The large volume of
the pig brain (75 mL) is sufficient for the discernment of the
anatomical structure by PET, and we have already described in
some detail the spatial pattern of binding of [11C]harmine to
MAO-A in the brain of living pig.16In the present study, we
tested the plasmas metabolism and cerebral uptake of 4 and 5
in anesthetized pigs. Specificity of, the binding of R-[11C]RO-
MAO in pig brain was tested in a blocking study performed by
the pretreatment of the animal with pargyline at a dose known
to block MAO-A in pig brain, 1 h before the tracer administration.
Results and Discussion
Chemistry. The syntheses of (R)-(-)- and (S)-(+)-1-(1-
methyl-1H-pyrrol-2-yl)-2-phenyl-2-(1-pyrrolidinyl)ethanone (R-
and S-ROMAO, 2 and 3; Scheme 1) have been described
This synthetic pathway was not immediately transferable to
the synthesis of (R)-(-)- and (S)-(+)-2-phenyl-1-(1H-pyrrol-
2-yl)-2-(1-pyrrolidinyl)ethanone (desmethyl-R- and S-ROMAO,
6 and 7). Derivatives 6 and 7 were therefore obtained as
described in Scheme 2. The 1H-pyrrole underwent a Friedel-
–Crafts reaction with 2-chloro-2-phenylacetyl chloride to give
2-chloro-2-phenyl-1-(1H-pyrrol-2-yl)ethanone 8 in 20% yield.
This compound was treated with pyrrolidine in refluxing acetone
using K2CO3as a base, to obtain 6 and 7 in 20% yield. The
racemic mixture was separated by enantioselective high-
performance liquid chromatography (HPLC) at a semiprepara-
tive scale on a polysaccharide-based chiral stationary phase
(CSPa) (Chiralpack IA) to obtain the enantiomers 6 and 7
(80–90% yield) with enantiomeric excess (ee) > 99%, checked
by an analytical Chiralpack IA column (Figure 1).
Although the racemic mixture 6 and 7 was obtained in only
about 4% global yield, no efforts were made to improve this
yield, because the optimization of the synthesis was deemed
beyond the scope of the present study.
Stereochemical Characterization of the Enantiomers 6
and 7. The absolute configuration of 6 and 7 was assigned by
a comparison of their circular dichroism (CD) spectra with those
of the structurally related compounds in the ROMAO series
(Figure 2). As previously reported,23,27the dextrorotatory form
3 displays negative CD bands at around 254 and 319 nm and
two positive CD bands at around 229 and 290 nm. The CD
profile of the (+)-7 enantiomer in ethanol is strikingly similar
to that of reference compound (S)-(+)-ROMAO 3, while the
(-)-6 enantiomer showed opposite Cotton effects. Therefore,
we could empirically assign the S configuration to the dextroro-
tatory enantiomer 7 and the R configuration to the levorotatory
enantiomer 6.
Radiochemistry. The carbon-11 labeling of compound 6 to
obtain S-[11C]ROMAO (4) took 42 min ((5 min) (Scheme 3).
The same conditions were applied to transform its enantiomer
compound 7 into R-[11C]ROMAO (5). Yields were 1.2 GBq
((0.5 GBq) from 6 to 9 GBq [11C]CH3I. When the 20 min
physical half-life of carbon-11 was taken into account, the
calculated yield of the labeling reaction was 60–70%. The
products were 99% ((1%) radiochemically pure; the residual
content of precursor was below 50 µg/production; and the
contents of S- or R-ROMAO 4 or 5 were below 10 µg/
production. The specific activity was higher than 50 GBq/µmol.
MetabolicStability ofCarbon-11-Labeled
R-ROMAO (4 and 5). Four pigs were used for both PET scan
imaging studies and studies of the plasma metabolism of the
carbon-11-labeled S- and R-ROMAO (4 and 5). Each pig was
S- and
Scheme 1a
aReagents and conditions for the synthesis of isomer 2: (a)
CF3COOC2H5, tetramethyl-guanidine, MeOH, room temperature for 24 h,
93%, (b) Vilsmeier reactive, n-butyl acetate, -15 °C for 3 h, (c)
1-methylpyrrole, AlCl3, CH2Cl2, -5 °C for 2 h, 13%, (d) concentrated HCl,
MeOH, 40°C for 15 h, 80%, and (e) 1,4-dibromobutane, NaI, KHCO3,
reflux, 12 h, 67%. The same conditions were used to synthesize the isomer
3, using (S)-phenylglycine as the starting material.
Scheme 2a
aReagents and conditions: (a) AlCl3, CH2Cl2, -20 °C for 20 min and
(b) pyrrolidine, K2CO3, acetone, reflux for 96 h.
Journal of Medicinal Chemistry, 2008, Vol. 51, No. 6Jensen et al.
Page 3
Catheters had been placed in an femoral artery for blood
sampling and in a femoral vein for radiotracer injection. Arterial
blood samples were collected at intervals during PET scans for
pigs 2, 3, and 4 only for HPLC plasma metabolite measure-
ments. Carbon-11 analogues 4 and 5 were relatively stable
toward metabolism in the plasma of living pigs, compared to
[11C]harmine.16Metabolite HPLC analysis of the two stereoi-
somers shows no notable differences between the two isomers.
At 30 s after intravenous injection, 99.7% of the extracted
plasma radioactivity was the untransformed parent. This fraction
declined to 98% at 2 min, 68% at 10 min, 41% at 30 min, and
24% untransformed at 50 min. Reverse-phase radio-HPLC
analysis of the plasma showed that the radio-metabolite(s) eluted
early in the radiochromatogram, indicating that radio-metabo-
lite(s) are considerably more polar than the parent compound.
This predicts that the metabolites do not enter the brain,28which
is a precondition for kinetic modeling. Consequently, no attempt
was made to identify the polar plasma metabolites.
PET Examination of R- and S-[11C]ROMAO 4 and 5. The
anaesthetised pig was placed in the PET scanner before injection
of the radiotracer. On the basis of its higher selectivity for MAO-
A, we expected that R-[11C]ROMAO 4 might have superior
binding properties in the living brain when compared to its
enantiomer 5. Contrary to this expectation, maps of the
normalized uptake of the two enantiomers in pig 3 show that
Figure 1. (Traces a and b) Analytical separation of desmethyl-ROMAO
with UV and polarimetric detection. (Traces c and d) Purity control of
the first and second fractions collected at a semipreparative scale. k1,
retention factor of the first eluted enantiomer, defined as (t1 - t0)/t0,
where t0 is the dead time of the column. R, enantioselectivity factor
defined as k2/k1. Rs, resolution factor defined as 2(t2- t1)/(w1+ w2),
where t1and t2are retention times and w1and w2are band widths at
the baseline in time units.
Figure 2. CD profiles of reference compound (S)-(+)-ROMAO (3)
and the enantiomers of desmethyl-ROMAO (6 and 7) in ethanol.
Scheme 3a
aReagents and conditions: (a)11CH3I, NaH, DMF, room temperature
for 1 min. The same conditions were used to obtain 5 using 7 as the starting
Figure 3. Normalized uptake of the R- and S-[11C]ROMAO 4 and 5
in pig 3. The values are the average concentrations of the interval
between 10 and 60 min, as a percentage of the injected dose per liter
(% ID/L). Extracerebral voxels have been removed, and the images
are blurred (4 mm fwhm). Maps are shown in coronal, sagittal, and
horizontal planes.
NoVel Tracer for PET Studies of MAO-AJournal of Medicinal Chemistry, 2008, Vol. 51, No. 6 1619
Page 4
net uptake of the S form 5 was 2-fold greater than for the R
form 4 (Figure 3). The specific activities of the two injections
were both very high (>100 GBq/µmol), and the dose injected
was very similar, at 480 MBq ((20 MBq). Compounds 4 and
5 have very similar metabolic patterns; therefore, differences
in plasma metabolism cannot explain the higher brain uptake
of the S form compared to the R form.
Comparison of the parametric maps of the equilibrium
distribution volume (Vd,amL g-1) relative to the metabolite-
corrected arterial input for 4 and 5 (R- and S-[11C]ROMAO)
with previously obtained results for [11C]harmine16showed that
all three tracers had preferential radioactivity accumulation in
the ventral forebrain/ventral striatum. In the parametric maps
of 4 and 5 (R- and S-[11C]ROMAO) Vdhad only this single
"hotspot" (Figure 3), in contrast to [11C]harmine, which had a
somewhat more complex pattern of cerebral binding.
To verify the pharmacological nature of its binding in brain,
a blocking PET study was carried out with 4 in pig 4. A baseline
recording was followed by a second scan, initiated at 1 h after
pargyline treatment (5 mg/kg, i.v.). At intervals after tracer
injection, we measured the radioactivity in (i) whole arterial
blood, (ii) plasma, and (iii) acetonitrile precipitate of plasma.
The extract after acetonitrile precipitation plasma was examined
by HPLC to measure the metabolism at selected times. The Vd
maps were calculated as relative to the untransformed tracer in
the plasma extracts. The fraction precipitated with acetonitrile
was reduced to 25–30% after treatment with pargyline, sug-
gesting that bound R-[11C]ROMAO (4) can be trapped in a
plasma protein compartment by a MAO-dependent process.
Taking into account the above, we used the following equation
to generate the arterial input concentration as a function of the
circulation time (arterial input function, Ca(T)):
where PR(t) is the total plasma radioactivity, PPR(t) is the
plasma precipitate radioactivity, and ftraceris the unmetabolized
parent radioligand, measured by radio-HPLC. This equation
corrects for both protein binding and metabolism of the free
The first term of eq 1 referred to the measured plasma
radioactivity (PR); the second term [PR(t) - PPR(t)/PR(t)] takes
into account the amount unavailable for brain uptake because
of protein binding; and the third term (ftracer) considers the
removal of the metabolites from the input function.
In our previous study using [11C]harmine to visualize the
MAO enzyme, we found that the Vdin the pituitary gland was
partly resistant to pargyline blockage, suggesting some phar-
macological heterogeneity in that structure.16However, [11C]RO-
MAO (4 nor 5) did not discernibly label the pig pituitary; indeed,
with [11C]ROMAO (4 or 5), the only region with distinctly
greater binding was the ventral forebrain (Figure 4), although
there was some evidence of greater uptake in the diencephalon.
The blocking study revealed a global fall in Vdthroughout out
the brain, which was most conspicuous in the ventral forebrain,
consistent with the global pattern of distribution of MAO-A.
On the basis of these preliminary results, both R- and
S-[11C]ROMAO 4 and 5 appear to be very promising PET
tracers for the MAO-A enzyme in brain. There are several
positive attributes to these ligands, which may present advan-
tages over [11C]harmine and other existing ligands. In particular,
the carbon-11-labeling reaction is fairly simple and robust.
Yields of more than 1 GBq were routinely obtained and with
high specific activity of the final product. The metabolism of
R- and S-[11C]ROMAO (4 and 5) is relatively slow in plasma
of living pigs, in contrast to [11C]harmine, which is difficult to
detect in plasma at times after 10 min. Parametric maps of
[11C]ROMAOs 4 and 5 binding are qualitatively very compa-
rable to those of [11C]harmine.16There was extensive displace-
ment of binding after the blockade of the MAO-A enzyme with
pargyline at a dose know to block entirely [11C]harmine binding
in pig brain.
Experimental Section
Chemistry. General. Melting points were determined with a
Buchi 530 capillary apparatus and are uncorrected. Infrared (IR)
spectra were recorded on a Spectrum-one spectrophotometer.1H
NMR spectra were recorded on a Bruker AC 400 spectrometer,
using tetramethylsilane (Me4Si) as an internal standard. All
compounds were routinely checked by thin-layer chromatography
(TLC) and
performed using aluminum-baked silica gel plates (Fluka F254) and
aluminum-baked aluminum oxide plates (Fluka F254). The concen-
tration of solutions after reactions and extractions was obtained
using a rotatory evaporator operating at a reduced pressure of
approximately 20 Torr. Organic solutions were dried over anhydrous
sodium sulfate. Solvents and reagents were obtained from com-
mercial suppliers (Sigma-Aldrich) and were used without further
(8). AlCl3(9.9 g, 74.2 mmol) was added in small portions during
30 min into a well-stirred solution of pyrrole (10 g, 149.0 mmol)
and 2-chloro-2-phenylacetyl chloride (14.1 g, 74.5 mmol) in CH2Cl2
(120 mL) cooled at -20 °C. The mixture was stirred at -20 °C
for 20 min, and then the mixture was added to a slush consisting
1H nuclear magnetic resonance (NMR). TLC was
Figure 4. Parametric images of R-[11C]ROMAO (4) distribution
volume in pig 4 before (A) and after (B) pargyline. Extracerebral voxels
have been removed, and the images are blurred (4 mm). Maps are shown
in coronal, sagittal, and horizontal planes.
Journal of Medicinal Chemistry, 2008, Vol. 51, No. 6 Jensen et al.
Page 5
of crushed ice (250 g) and concentrated HCl (17 mL). Extraction
with CHCl3(3 × 200 mL) provided an organic solution that was
washed with brine (3 × 250 mL), saturated NaHCO3solution (1
× 250 mL), then with brine again (3 × 250 mL), before it was
dried. Removal of the solvent by evaporation yielded a residue,
which was chromatographed on an alumina column (chloroform
as the eluent) to afford pure 8 (racemic mixture) (3.27 g, 20%).
For analytical data, see the Supporting Information.
and 7) (Desmethyl-ROMAO). A solution of 8 (2.6 g, 11.6 mmol)
in acetone (52 mL) was added dropwise into a well-stirred
suspension of pyrrolidine (1.6 g, 23.1 mmol) and K2CO3(3.0 g,
21.6 mmol) in acetone (52 mL). The mixture was refluxed with
stirring for 96 h and then treated with water. After extraction with
CHCl3(100 mL), the organic solution was washed with brine (5 ×
50 mL) and dried. Evaporation of the solvent gave a crude product,
which was chromatographed on a silica column (5:1 ethyl acetate/
chloroform as the eluent) to afford 6 and 7 (racemic mixture) (590
mg, 20%). For analytical data, see the Supporting Information.
Enantioseparation. HPLC-grade solvents were supplied by
Carlo Erba (Milan, Italy). Diethylamine (DEA) was obtained from
Fluka Chemie (Buchs, Switzerland). HPLC enantioseparations were
carried out by using a Perkin-Elmer (Norwalk, CT) 200 liquid
chromatographic pump equipped with a Rheodine (Cotati, CA)
injector with a 20 µL (analytical) or 1 mL (semipreparative) sample
loop, a Perkin-Elmer column oven, and a Perkin-Elmer 290 UV
detector. The sign of the optical rotations of enantiomers of
compounds tested was measured online at a wavelength of 365
nm by a Perkin-Elmer polarimeter model 241 equipped with Hg/
Na lamps and a 40 µL flow-cell. The signal was acquired and
processed by Clarity (Data Apex, Prague, Czech Republic) software.
The chiral analytes were dissolved in ethanol, and their CD spectra
were measured in a quartz cell (0.1 cm path length) using a Jasco
J-710 spectropolarimeter (Japan Spectroscopic, Tokyo, Japan),
maintained thermostatically at 25 °C. The mean spectra from three
instrumental scans were calculated as ellipticity values (millide-
Semipreparative enantioseparations of 6 and 7 (desmethyl-
ROMAO) were accomplished by HPLC on a polysaccharide-based
chiral stationary phase (CSP) column type Chiralpak IA 250 ×
4.6 mm I.D., with a mixture of 60:40:1:0.1 n-hexane/ethyl acetate/
ethanol/DEA (v/v/v/v) as the eluent. The column temperature was
set at 25 °C, with a flow rate of 4 mL/min and detection wavelength
at 280 nm. After each semipreparative chromatographic run (25
mg), the fractions corresponding to a single enantiomer were pooled
and evaporated. The collected fractions of each enantiomer were
analyzed by analytical Chiralpak IA chiral columns, with a flow
rate at 1.0 mL/min, at 25 °C with UV detection at 280 nm, to
determine their enantiomeric excess (ee > 99%). The yields ranged
from 80 to 90% (Figure 1).
Animals, Anesthesia, Surgery, and PET Scanning. The study
procedure was approved by the Danish Experimental Animal
Inspectorate. A total of 4 Danish Landrace/Yorkshire pigs (females;
38.25–40.5 kg of body weight; approximately 3 months old) were
used. For experimental details, see the Supporting Information.
In three cases, the pigs had been used for other PET-scanning
protocols before this study. They had previously been treated with
methyl-3-pyridinecarboxamide hydrochloride (GSK189254) (pig 1),
quinolin-2-one (aripiprazole) (pig 2), and 3,5-dichloro-N-[(1-
ethylpyrrolidin-2-yl)methyl]-2-hydroxy-6-methoxy-benzamide (raclo-
pride), 3-(2-[4-(4-fluorobenzoyl)-1-piperidinyl]ethyl)-2,4(1H,3H)-
quinazolinedione tartrate salt (kentaserin), 2-(5-methoxy-1H-indol-
azabicyclo[2.2.2]oct-5-yl)- (6-methoxyquinolin-4-yl)-methanol
(quinidine) (pig 3) at pharmalogically active doses, in the hours
prior to scanning with [11C]ROMAO (4 or 5). The fourth pig was
dedicated to the [11C]ROMAO (4 or 5) study. After acquisition of
a brief attenuation scan, 90 min long dynamic emission recordings
were performed in the aperture of the Siemens ECAT Exact
(MeO-DMT), 2-ethenyl-4-
tomograph. Pig 4 was scanned with R-[11C]ROMAO (4) before
and after blocking with pargyline. Blood samples were collected
during scans for pigs 2, 3, and 4 for metabolite measurements.
Radiochemistry. Acetonitrile (HPLC), dichloromethane, iodine,
sodium phosphate monohydrate, and bufotenine (1 mg/mL) in
acetonitrile were purchased from Bie and Berntsen, Denmark.
Pargyline HCl, were obtained from Sigma-Aldrich, Denmark. The
synthetic route to obtain (R)-(-)- and (S)-(+)-1-(1-[11C]methyl-
MAO 4 and 5 is shown in Scheme 3. [11C]Carbon dioxide was
prepared by a General Electric Medical Systems PET trace 200
cyclotron by a14N(p,R)11C nuclear reaction. [11C]carbon dioxide
was reduced to [11C]methane, which was then converted to
[11C]methyl iodide using the General Electric methyl iodide box.
The [11C]methyl iodide (6–9 GBq after 30 min of bombardment at
40 µA) was transferred in a stream of nitrogen and bubbled though
the reaction mixture in a capped vial via a needle and vent needle.
The premade reaction mixture was a suspension containing the
precursor (6 or 7, ca. 1.5 mg) and NaH (0.3 mg) in DMF (300
µL); it was shaken before the [11C]methyl iodide gas was bubbled
through it. The reaction mixture was left for 1 min at room
temperature before it was quenched with HPLC eluent (0.3 mL)
and transferred into the HPLC loop.
The reaction mixture was purified using a semipreparative HPLC
(PerkinElmer model 200) equipped with a 5 mL injection loop.
Product elution was monitored with online γ detection of in-house
design, in conjunction with UV-visible detection (Applied Bio-
systems model 759A, λ ) 300 nm). The mobile phase, consisting
of 30–40% ammonium formate (0.1 M, pH adjusted to 8.5 with
NaOH) and 60–70% acetonitrile, was delivered at a rate of 10 mL/
min to a SpherClone ODS (2) column. In both cases, the products
eluted as broad peaks: the first one has Rf of 10–16 min (40%
acetonitrile), and the second one has Rf of 25–32 min (30%
acetonitrile). The fraction corresponding to the labeled product (ca.
25 mL) was collected, concentrated to ca. 5 mL under reduced
pressure at 90 °C, and formulated with isotonic saline solution (5
The purity of the final product was determined by HPLC, using
a BondClone C18 column (5 µm, 250 × 4.6 mm) with an eluent
consisting of 50% aqueous 0.1 M ammonium formate (pH 8.5 with
NaOH) and 50% acetonitrile, with serial UV (250 nm) and γ
detection. Reference standards of ROMAO (50 µg/mL) (2 or 3)
and desmethyl-ROMAO (6 or 7) (50 µg/mL) were used for
quantification. The product solution was spiked with ROMAO (2
or 3) in a second run to verify product identity on the basis of the
co-elution position of the UV peak and the radioactivity peaks.
Pharmacokinetic Analysis. No arterial input was obtained for
pigs 1–3; therefore, the analysis of tracer uptake in these three pigs
were limited to the comparison of the region of interest (ROI)
analysis of the summation images. For pig 4, continuous arterial
inputs of untransformed R-[11C]ROMAO (4) and the sum of its
metabolites were calculated. The area under the curve for plasma
R-[11C]ROMAO (4) as a percentage of the total injected dose was
calculated for the interval of the PET recording (90 min). Plasma
metabolite analysis in pigs 3 and 4 was made from arterial blood
samples (1 mL) drawn at 0.5, 2, 5, 10, 20, 30, 40, 50, 70, and 90
min after injection. The blood samples were centrifuged (1300g)
for 1 min, and then 0.5 mL of the plasma was denatured by mixing
with 0.5 mL of acetronitrile. The mixture was shaken and then
centrifuged (1300 g) for 5 min. The supernatants were analyzed
using a BondClone C18 column (5 µm, 250 × 4.6 mm) with an
eluent consisting of 50% aqueous 0.1 M ammonium formate (pH
8.5 with NaOH) and 50% acetonitrile, with serial UV (280 nm)
and γ detection at a flow rate of 2.5 mL/min. The radioactivity of
the precipitate was measured in a well counter cross-calibrated to
the tomograph.
Voxel-wise parametric maps of the distribution volume (Vd, mL/
g) of R-[11C]ROMAO (4) were calculated in the native PET space
relative to the metabolite-corrected arterial input by the method of
Turkheimer et al.29for pig 4. In this method, Akaike-weighted
averages of the estimates of Vdfor one- and two-tissue compartment
NoVel Tracer for PET Studies of MAO-AJournal of Medicinal Chemistry, 2008, Vol. 51, No. 6 1621
Page 6
models were obtained in each voxel using linearized equations.30
The sum of all emission frames from each PET session was
calculated and manually registered to the MR-based common
stereotaxic space for the minipig brain,29using a rigid-body
transformation with 9 degrees of freedom in "Register".31Visual
inspection of the individual dynamic and summed emission
recordings did not reveal discernible head movement in the course
of each PET session. Therefore, individual parametric maps of Vd
were resampled into the common stereotaxic space using the
transformation matrix and averaged by condition.
Acknowledgment. This project was supported by the Italian
MUR and Denmark's National Science Foundation.
Supporting Information Available: Analytical data on com-
pounds 6-8 and experimental data on animal handling. This
material is available free of charge via the Internet at http://
(1) Schoepp, D. D.; Azzaro, A. J. Specificity of endogenous substrates
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C. D.; Langstrøm, B.; Alexoff, D. Selective, irreversible in vivo binding
of [11C]clorgyline and [11C]-L-deprenyl in mice: potential for measure-
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(6) Lammertsma, A. A.; Bench, C. J.; Price, G. W.; Cremer, J. E.; Luthra,
S. K.; Turton, D.; Wood, N. D.; Frakowiak, R. S. J. Measurement of
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1991, 11, 545–556.
(7) Fowler, J. S.; Wolf, R. R.; MacGregor, R. R.; Dewey, S. L.; Logan,
J.; Schlyer, D. J.; Langstrom, B. Mechanistic positron emission
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the monoamine oxidase catalyzed binding of [11C]L-deprenyl in living
baboon brain. J. Neurochem. 1988, 51, 1524–1534.
(8) Fowler, J. S.; Wang, G. J.; Logan, J.; Xie, S.; Volkow, N. D.;
MacGregor, R. R.; Schlyer, D. J.; Pappas, N.; Alexoff, D. L.; Patlak,
C.; Wolf, A. P. Selective reduction of radiotracer trapping by deuterium
substitution: Comparison of carbon-11-L-deprenyl and carbon-11-
deprenyl-D2 for MAO B mapping. J. Nucl. Med. 1995, 36, 1255–
(9) Logan, J.; Fowler, J. S.; Ding, Y. S.; Franceschi, D.; Wang, G. J.;
Volkow, N. D.; Felder, C.; Alexoff, D. Strategy for the formation of
parametric images under conditions of low injected radioactivity
applied to PET studies with the irreversible monoamine oxidase A
tracers [11C]clorgyline and deuterium-substituted [11C]clorgyline.
J. Cereb. Blood Flow Metab. 2002, 22, 1376–1376.
(10) Ohmomo, Y.; Hirata, M.; Murakami, K.; Magata, Y.; Tanaka, C.;
monoamine oxidase-A specific inhibitor fpr positron emission tomog-
raphy. Chem. Pharm. Bull. 1993, 41, 1994–1997.
(11) Mukherjee, J.; Yang, Z.-Y. Development of N-[3-(2′,4′-dichlorophe-
noxy)-2-18F-fluoropropyl]-N-methylpropargylamine (18F-fluoroclor-
gyline) as a potential PET radiotracer for monoamine oxidase-A. Nucl.
Med. Biol. 1999, 26, 619–625.
(12) Mukherjee, J.; Yang, Z.-Y. Monoamine oxidase A inhibition by
fluoxetine: an in vitro and in vivo study. Synapse 1999, 31, 285–289.
(13) Hamid, R.; Chalon, S.; Ombetta, J.-E.; Frangin, Y.; Garreau, L.;
Dognon, A.-M.; Lena, I.; Bodard, S.; Vilar, M.-P.; Besnard, J.-C.;
Guilloteau, D. Synthesis and characterization of [125I]N-(2-aminoet-
hyl)-4-iodobenzamide as a selective monoamine oxidase B inhibitor.
Nucl. Med. Biol. 1995, 22, 617–623.
(14) Bergström, M.; Westerberg, G.; Långström, B.11C-Harmine as a tracer
for monoamine oxidase A (MAO-A): In vitro and in vivo studies.
Nucl. Med. Biol. 1997, 24, 287–293.
(15) Bergström, M.; Westerberg, G.; Nemeth, G.; Traut, M.; Gross, G.;
Greger, G.; Müller-Peltzer, H.; Safer, A.; Eckernäs, S.-Å.; Grahnr,
A.; Långström, B. MAO-A inhibition in brain after dosing with
esuprome, moclobemide, and placebo in healthy volunteers: In vivo
studies with positron emission tomography. Eur. J. Clin. Pharmacol.
1997, 52, 121–128.
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monoamine oxidase inhibition on amphetamine-evoked changes in
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study with [11C]harmine and [11C]raclopride. Synapse 2006, 59, 427–
(17) Meyer, J. H.; Ginovart, N.; Boovariwala, A.; Sagrati, S.; Hussey, D.;
Garcia, A.; Young, T.; Prashak-Rieder, N.; Wilson, A. A.; Houle, S.
Elevated monoamine oxidase A levels in the brainsAn explanation
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Psychiatry 2006, 63, 1209–1216.
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Barmoulle, Y.; Curet, O.; Jegham, J.; Pinquier, J. L.; Valette, G. P.
Mapping the cerebral monoamine oxidase type A: Positron emission
tomography with the reversible selective inhibitor [11C]befloxatone.
J. Pharmacol. Exp. Ther. 2003, 305, 467–473.
(19) Dolle, F.; Valette, H.; Barmoulle, Y.; Guenther, I.; Fuseau, C.; Coulon,
C.; Lartizien, C.; Jegham, S.; George, P.; Curet, O.; Pinquier, J. L.;
Bottlaender, M. Synthesis and in vivo imaging properties of [11C]be-
floxatone: A novel highly potent positron emission tomography ligand
for mono-amine oxidase-A. Bioorg. Med. Chem. Lett. 2003, 13, 1771–
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Shea, C.; Alexoff, D.; mecGregor, R. R.; Schlyer, D. J.; Zezulkova,
I.; Wolf, A. P. Brain monoamine oxidase A inhibition in cigarette
smokers. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 14065–14069.
(21) Fowler, J. S.; Volkow, N. D.; Wang, G. J.; Pappas, N.; Logan, J.;
MacGregor, R.; Alexoff, D.; Wolf, A. P.; Warner, D.; Cilento, R.;
Zezulkova, I. Neuropharmacological actions of cigarette smoke: Brain
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(23) Di Santo, R.; Costi, R.; Roux, A.; Artico, M.; Befani, O.; Meninno,
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Hansen, A. K. The use of pigs in neuroscience: Modeling brain
disorders. Neurosci. BiobehaV. ReV. 2007, 31 (5), 728–751.
(25) Pedersen, K.; Simonsen, M.; Ostergaard, S. D.; Munk, O. L.; Rosa-
Neto, P.; Olsen, A. K.; Jensen, S. B.; Moller, A.; Cumming, P.
Mapping the amphetamine-evoked changes in [11C]raclopride binding
in living rat using small animal PET: Modulation by MAO inhibition.
Neuroimage 2007, 35 (1), 38–46.
(26) Jensen, S. B.; Smith, D. F.; Bender, D.; Jakobsen, S.; Peters, D.;
Nielsen, E. O.; Olsen, G. M.; Scheel-Kruger, J.; Wilson, A.; Cumming,
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transporters in living porcine brain. Synapse 2003, 49, 170–177.
(27) La Torre, F.; Cirilli, R.; Costi, R.; Di Santo, R.; Ferretti, R.; Gallinella,
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(30) Zhou, Y.; Brasic, J. R.; Ye, W.; Dogan, A. S.; Hilton, J.; Singer, H. S.;
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McConnell Brain Imaging Center: Montreal, Canada, 1986.
Journal of Medicinal Chemistry, 2008, Vol. 51, No. 6Jensen et al.


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An Example of a Library Marketing Plan document sample
About Data Instruction Text Question Have you taken or are you Have you currently taking written a Please indicate How did you find this a Marketing Marketing your status: Marketing Plan Tutorial? Class? Plan before? Participant IDStart Date Finish Date 12/14/2006 11:24 1330599 12/14/2006 11:02 AM AM UWB Student Suggested in class Yes Yes 12/06/2006 11:22 1310243 12/06/2006 11:20 AM AM UWB Student Suggested in class Yes No 1290538 11/28/2006 2:21 PM 11/28/2006 2:23 PM UWB Student Suggested in class Yes No 10/31/2006 11:03 Other please explain: 1230043 10/31/2006 10:58 PM PM UWB Faculty Doreen emailed the link 999 No How easy was it to Would you use was this How clearly was the recommend the Is there anything you would How helpful was Marketing Plan information Marketing Plan like to change about the the Resources Tutorial? presented? Tutorial to others? Tutorial? page? Any further comments or suggestions: Yes, it was helpful to get the guidance and link to the specific part, for example the Situational Analysis-there are few examples that help clear things up. And, every step is Very easy Clearly Yes Yes, please explain: Helpful easy to understand and straight to the point. Somewhat easy Somewhat clearly Yes No Somewhat helpful 999 Somewhat easy Clearly Yes No Helpful 999 There are alternate requirements for a marketing plan, depending whether it's for one of our classes vs. doing it for a real live business vs. starting a business. For example, I like forcing people to derive the the "magic number". Yes, please explain: There is Anyways, kudos to both of you. This is far always something to add, but better than many things that come across I'm afraid it would add to the my desk! Very easy Clearly Yes complexity Helpful BTW, where's my candy bar???!!!
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Cloud Atlas
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Super Schmoe
Join Date: Sep 2001
Location: Ottawa, ON
Posts: 6,407
Favorite Movie: Dazed & Confused
Argh I want to see this so badly but I promised a friend months ago I'd see it with him and some other ppl when he came to town. So I gotta wait till next Sunday to see it :/.
Early numbers put it on track to make $13 million this weekend. A bit disappointing but not unexpected especially with the lower theatre count. Hopefully word of mouth is good and helps it spread.
While I think it's important for movie fans to put their money where their mouth is (you want something different from the normal Hollywood junk, at least give this movie a chance!), that doesn't necessarily mean Cloud Atlas will be unsuccessful overall. Its worldwide prospects are far rosier as other continents tend to be more receptive to odder films like this. Tykwer's Perfume made $130 million worldwide and only $2 million in the U.S. and this should have broader appeal, with more names in the cast. I think ultimately Cloud Atlas will be pretty profitable, even if it does poorly in North America but I'm still hoping it does well here as well.
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- Pasta Lamp (2009)- table lamp series-
- Pasta is architecture designed with five senses. Every single shape is like a brick of architecture, different in form, consistency and color, to creat extraordinary construction, both physically and mentally.
5 lamps is a conceptual design project in which I created five lamps using five different typed of Italian pasta. The project is concentrated in making lampshades which are represented how every single piece of pasta would be transformed into some kind of natural elements with light and shade from original pasta's figure in those five different "cookings".


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Italian cyclist Matteo Trentin timed his finish perfectly to win the hilly 14th stage of the Tour de France, while Chris Froome preserved his overall lead by staying in the pack on Saturday.
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85 Tauren Druid
Well after 6 years of raiding I have finally had to put it aside. I have not decided if I am still even going to play yet.
I just wanted to thank everyone who has made this game that much more worth it for me through the years.
Kamah let me into his guild in shen, killacow was the biggest !@# officer ever, and made me farm for him but i learned after this.
After shen was Vac which was a interesting exp.
The biggest guild I had was Second string and was probly the most fun I have ever had online. Between the screaming boots in BT to the random funny moments we had it was awesome.
Then of course Nothing Fancy. I have been raiding with some of these guys for years.
Bigpapi: Oh wat can we say its papi, even though you dnt have a job I still love you.
Rosh:Had some good times together in and out of raid with just some crazy $%^-
Raile:best Disp priest in rated bgs cant believe some of the saves you had.
Morg:frenchy nuff said
Dufferz: probly some of the most fun I have had in keybind vent chat lol
Gorrath hell of a good tank even though i tanked most of the time.
Pup: how you havnt died from a heart attack from raiding is beyond me but I got to say everyday you stayed with it.
Jess:You stayed by me through thick and thin in my real life and still have and that i love you for.
Honey/Fog: you both are goofy and good healers, same with raile.
pooner: though we raided some early and some late you were always a guy I could go to for personal help or a ear to vent to.
Dak: Wintergrasp fun !
Dusen: I have known this guy for ever and I think cause of him i might not cancel my account completly, not sure though.
I could go on and on but then the trolls would have to much food.
Long story short spent alot of time with alot of good people. I have to move on and either become casual or quite all the way. Always have to remember Life>WOW and mine could not get better right now. So take care and I might pop in to say hi to the people that have been there for me.
Well this bear druid is tired Ttyl
good luck in life toad. you've changed so much, for the better, since rydumo introduced us when you were just a noob. it's been a pleasure gaming with you. keep in touch.
on the bad officer thing, sorry about that, i knew i screwed the pooch on officers around that time. but, the whole farming thing is news to me. i'm sorry? =]
Edited by Kamah on 9/17/2012 1:40 PM PDT
90 Night Elf Druid
90 Tauren Druid
Ok, see you in 10 minutes.
Also I resent sharing a blurb with Jake.
90 Human Warrior
We never really associated being we were always opposite faction, but I wish you nothing but the best. Take care of yourself & good luck!
100 Tauren Shaman
Thanks for always being nice when I went /fanboi
90 Tauren Shaman
Honey/Fog: you both are goofy and good healers, same with raile.
Hmmm, I guess this was a hint that I wasn't a good healer! : ) All kidding aside, it was a lot of fun raiding with you! You know I always enjoy and love helping people, so feel free to hit me up if you want me to lend an ear for you! Enjoy your new location and life!
90 Undead Death Knight
Good luck with whatever it is you are moving on to.
but toad what if we make our next get together close enough so you n epi can go? j/s I'm prettier sure thats close enough that fog n honey could come too. Puppy doesn't wanna have to drive that far cause he thinks some southerner with lynch tree him and/or that he'll get arrested as a terrorist at the airport, but we're working on it. Maybe just find someone with a big enough trunk he can just hide it in...so like a smart car for the little guy... or we can dress up his white wife like homeland security and pretend like she's taking him in. THey might let him pass safely then.
89 Troll Druid
yes ahahhaha
90 Undead Hunter
90 Worgen Hunter
Good luck with your future choices Gtoad. I'm pretty sure there are going to be multiple people that are going to miss you and your tanking ability. Have fun.
Threats of violence. We take these seriously and will alert the proper authorities.
Posts containing personal information about other players. This includes physical addresses, e-mail addresses, phone numbers, and inappropriate photos and/or videos.
Harassing or discriminatory language. This will not be tolerated.


text:
Who will skate in the blue ice for the Habs?
Habs coach Guy Carbonneau faces a tough decision after a Game 3 loss to Philly -- stick with future star Carey Price or go to untested Jaroslav Halak? Scott Burnside tells us what he learned about the Canadiens netminding after Monday's game.
1. Wouldn't necessarily want to be in Guy Carbonneau's shoes this morning. The head coach of the suddenly floundering Montreal Canadiens has to decide whether to go back to rookie netminder Carey Price in Game 4 on Wednesday night after Price was torched for three goals on six shots in the second period Monday as Philadelphia downed Montreal for the second straight time by a 3-2 count. The Flyers now lead the series 2-1, and Price has allowed 10 goals on 68 shots and simply isn't playing well enough to give the Habs a chance to win. Perhaps more troubling, Price has given up seven even-strength goals and one short-handed goal in a series in which the Habs have owned a huge territorial advantage. The alternative to Price is another rookie, Jaroslav Halak, who got his first taste of NHL playoff action in the third period Monday. He faced two shots. Given that Price is the goalie of the future, expect him back between the pipes, but both his confidence and the confidence of his teammates to get the job done has to be waning.
4. When the Dallas Stars and San Jose Sharks meet Tuesday night, it will feature a battle of two big trade-deadline acquisitions who are headed in opposite directions. San Jose defenseman Brian Campbell was the top defenseman on the move in February, and he will be the most sought-after defender when free agency begins July 1. But both Campbell, who had 19 points in 20 regular-season games after the trade, and the Sharks have struggled to replicate their torrid pace during the final weeks of the regular season. They struggled against Calgary in the first round and now face a 2-0 deficit against Dallas, having lost the first two games of the series at home. Meanwhile, Brad Richards, the former Conn Smythe (playoff MVP) winner with Tampa Bay who came to Dallas at the deadline, collected four third-period points in Game 2 to help the upstart Stars erase a 1-0 Sharks' lead. The Stars have won four of five road games this spring, and Richards has nine points in eight games and is plus-4 for the Stars.
5. Want an idea of why the Flyers have been successful against Montreal -- beyond Martin Biron's heroics in the Flyers' goal? Well, look no further than the team concept when it comes to scoring: Seven Flyers have scored 10 times in three games. R.J. Umberger is doing his best Wayne Gretzky imitation with four goals in the series, but the balanced production is a key to the Flyers' early success against the Habs. On Monday night three different players -- Umberger, Scottie Upshall and Mike Richards -- tallied in the 3-2 victory.
Scott Burnside is the NHL writer for ESPN.com.
- Hockey writer for ESPN.com
- 10 years at Toronto Sun, Windsor Star and National Post
- Wrote best-selling true crime book "Deadly Innocence"
We're down to the final eight teams as the battle for the Stanley Cup continues. Scott Burnside previews the second round:
• No. 1 Montreal vs. No. 6 Philadelphia
• No. 2 Pittsburgh vs. No. 5 N.Y. Rangers
• No. 1 Detroit vs. No. 6 Colorado
• No. 2 San Jose vs. No. 5 Dallas
• 2008 NHL playoffs index
- Reebok Men's St. Louis Blues Jaroslav Halak #41 Navy Alternate Premier Jersey
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Sleep Inn Lake Wright - Norfolk Airport
6280 Northampton Blvd
Norfolk, VA 23502
Phone: (757) 461-1133
Fax: (757) 461-5925
Azalea Inn
2344 E Little Creek Road
Norfolk, VA 23518
+1 757 587 4649
Su to Th from 11:00 AM to 11:00 PM,Fr to Sa from 11:00 AM to 01:00 AMThis Greek and Italian restaurant, located inside the Roosevelt Gardens Shopping Center serves up what some locals call the best pizza around. In addition to good portions and good service, the Azalea Inn also features a game room for the kids. The Azalea Inn is also a lunchtime favorite. Another favorite at the Azalea is the Spanikopita.
Earn valuable points toward free nights.


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Graal Hotel Ravello
No availability found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Shuttle bus service
- Continental Breakfast
- Beach
- Restaurant
- Free High-Speed Internet
- Room service
Photos of Graal Hotel Ravello
Travelers also recommend:
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Travel Blogs from Ravello
Ravello - Day 33
... of the spectacular coastline on the trip to Ravello because I had some guys smelly armpits in my face. Ravello is spectacular - the views are amazing. I did a tour of two villas while I was there (Jack didn't - he wasn't interested). The first was Villa Cimbrone - 6 hectares of gardens, fountains, nymphs, temples, pavilions, and of course, those stunning views. The second was Villa ...
Ravello and Amalfi
... the front of the queue and could get seats on the right side of the bus for the best views. Waited for the 9.15 bus which didn't come, the next bus at 9.30 didn't turn up either. Eventually one of the bus officials came to tell us that the next bus was at 9.50. By this time the bus queue was Bout 1/2 km long! At least we were near the front so we knew we would get on. At last the bus arrived and off we went. The trip was hair raising, the road is so windy Nd you ...
Day 19 Day trip to Ravello and Villa Cimbrone
Yet another lovely blue sky morning in Positano. The plan for today is trip to Ravello and Villa Cimbrone. Ravello is a music and arts mecca; its spectacular views over the gulf of Salerno and coastal towns and its two main garden villas Cimbrone and Rufolo are an attraction to historical and current artists and source of inspiration.
The trip ...
No Roadwork Ahead
... left at the apartment that we might want to take a hiking tour on the only road in Ravello that doesn't go anywhere!
Around 9 p.m. we were discussing the day events and Chuck asked if we'd seen any women driving here and we said not many but a few. We asked why and he remarked that he hadn't seen any. "Whenever I look up, all I see are men glaring at me."
OK, it wasn't the Amalfi Coast afterall
... new trails. Instead we were on a lovely road through wooded areas, nice hills and stunning scenery. Suddenly, a road closed sign appeared in our path. In the past we have just gone around the signs unless there happened to be a police officer yelling at us to turn around. It always worked in the past. This time, however, there were concrete blockades and we were about to head into a tunnel. The rational side of the brain kicked in and we opted to find a detour. The detour took us ...


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Daily Inspiration #38
Support Art|Spread Creativity|Get Inspired
A Creative Universe's Daily Inspiration
Source: ACreativeUnvierse.com
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3DUZEN ® 3X12. Thats all that will be made of these shirts. Only available in color shown. Sizes from small to xxlarge. Get em while they are fresh! There's only 36 and then they gone. 4ever! Buy Here.
Physalia Amphibious Garden by Vincent Callebaut
Architect Vincent Callebaut has designed a green, floating garden park that uses bio-filtration to clean the water upon which it floats. The Physalia Amphibious Floating Garden features four indoor/outdoor gardens that recycle river water in a natural life cycle. More at TheCoolist.
New Studio – Graphiatrist
Me and my friend Eric Ku have launched a studio together fresh for 2010. Hope you enjoy it. Find more work here.
Owen Freeman Illustrations
CLICK HERE for more images
Greg Williams Photography
CLICK HERE for more images
Peugeot RC1 concept car
A new concept car by Peugeot that will be unveiled in march, at the International Motor Show of Geneva.
More pictures of the Peugeot RC1 here.
Ariane Final Launch
[vimeo 8330730]
Ariane celebrates its 30th birthday this year ! Clip created by Un Oeil Sur Tout
On Thursday January 14th/20:00 I'll my exhibition in Hamburg/ Mono Gallery, it runs till February 14th. DJ Funkfinga will be responsible for the audio entertainment, I'll care for your visual pleasure, drinks are available.
You can check my stuff in my blog
If you read this/ are in the area/ got time feel invited!
Maxence Dedry – Photographer
20 years old belgian photographer Maxence Dedry shows a wide range of styles and techniques in his online portfolio.
Unlocking Beautiful Fantasies – Denise Grünstein
Step into the world of Denise Grünstein and get ready for a beautiful adventure. Her gorgeous photos make us feel as though we've been transported into another world. She has that rare ability to create interesting stories through a single picture or a whimsical series like some of our favorite photographers, Rodney Smith, Tim Walker, and Cade Martin. See more at My Modern Metropolis.
check for our artist bags on www.bagapart.com
many other stuff are on line


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Procrastinating Mt. Lemmon marigold bush prune job
The blooms are all gone and I'm ready to prune. Problem is I'm not sure how to handle this. Here are some excerpts I've read on Internet.
...should be pruned close to the ground...
...they can be sheared by one third in early summer...
...cut foliage back by approximately half during the early summer months...
I know there is at least one member of this forum who has this plant, Gxxxx. Here's a picture I snapped today:
The one thing I do know is to get rid of the dead wood. There are older, thicker gray branches, fresher green ones and some in between. Should I prune all equally? How much? Is there a certain point on the stems where I should make the cuts?
I'd appreciate advice on how to proceed.
You know, I'd snip off the older gray ones as close to the body of the plant as possible, and then give it a good, long stare to see how to proceed. If it was mine, I'd probably just quickly prune the remaining stems all to about 6 inches and then hope for the best. It'll fill back in quickly, I promise.
If you want something more natural and restrained looking right after the prune job (I really just hack mine back hard so immediately after it's not super natural looking, or attractive) you could get rid of the old stems, and shorten the younger stems in a random pattern, by cutting some back to 8 inches, and some to 4 inches, and some to 10 inches or so?
I'm sure mine would have looked better immediately after pruning had I taken more time with it, but generally I just hack them back wholesale in late winter.
Let us know what you do and how it works out! Even if you just left it alone I'm sure it would be a very attractive plant.
Good luck!
Grant, thank you for the quick and detailed instructions.
The deed is done. It went well. Bonus lesson: Don't prune on a windy day. Really, it looks okay. I read your advice and went to work. Took some thick branches way down and varied lengths of others. Everything down to 10" or less.
I really appreciate your response. You covered all the points I was unsure about. I'll post a picture next time it's in bloom. It feels so good to have it over with.


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I have a fiddle for this, but basically what it's doing it geo-encoding an address that is input into a textbox. After the address is entered and 'enter' is pressed, the dom does not immediately update, but waits for another change to the textbox. How do I get it to update the table right after a submit? I'm very new to Angular, but I'm learning. I find it interesting, but I have to learn to think differently.
Here is the fiddle and my controller.js
var myApp = angular.module('geo-encode', []);
function FirstAppCtrl($scope, $http) {
$scope.locations = [];
$scope.text = '';
$scope.nextId = 0;
var geo = new google.maps.Geocoder();
$scope.add = function() {
if (this.text) {
{ address : this.text,
region: 'no'
}, function(results, status){
var address = results[0].formatted_address;
var latitude = results[0].geometry.location.hb;
var longitude = results[0].geometry.location.ib;
$scope.locations.push({"name":address, id: $scope.nextId++,"coords":{"lat":latitude,"long":longitude}});
this.text = '';
$scope.remove = function(index) {
$scope.locations = $scope.locations.filter(function(location){
return location.id != index;


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Wisconsin's ban on gay marriage heads to federal appeals court today
Wisconsin and Indiana are looking to reinstate bans on gay marriage.
Sponsored by:
Wisconsin and Indiana are looking to reinstate bans on gay marriage.
Three boats were destroyed by a fire Monday evening.
The Common Core State Standards have Wisconsin officials divided–even within their own parties.
It was the third rally in eight days by demonstrators, who are angry over the shooting deaths of Dontre Hamilton in Milwaukee and Michael Br…
Barron County sheriff's officials the 61-year-old woman called authorities Sunday and said she had been hiking the trail near Haugen, but ha…
Michael Soeller lived alone in the apartment and his landlord found him after not seeing him for several days.
The request filed Saturday came after the 7th Circuit U.S. Court of Appeals' clerk mistakenly posted four documents online that weren't supp…
The Wisconsin Economic Development Corp. board quietly approved the tax credit for Ashley Furniture Industries.
Oral arguments will take place Tuesday before the 7th Circuit U.S. Court of Appeals.
Fitchburg police say the women were found shot in the Steele home.


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Rope-Seat Stools
Get MoreSubscribe to Martha Stewart Living
We used durable rock-climbing rope (available at sporting-goods stores; nylon rope from a hardware store is a strong, inexpensive alternative) in shades of orange and taupe to create dynamic -- and sturdy -- swirls of color. The tightly braided nylon rope is water- and stain-resistant, making it ideal for lunch at the kitchen counter or a barbecue by the pool.
- Rock-climbing or nylon rope
- Lighter
- Sandpaper
- Wooden stool
- Caulking gun
- Construction adhesive (available at hardware stores)
Step 1
To prevent fraying, singe one end of the rope with a lighter; then sand it to remove any black char.
Step 2
Lightly sand stool top. Using a caulking gun, gingerly apply construction adhesive to the stool's center. Roll a 3-inch spiral of rope to start, and glue it down by holding it for a few seconds until secure.
Step 3
Continue gluing and spiraling as you go, until the top and sides of the seat are covered. Snip off the end, singe and sand it, and glue it under the seat.


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Hi larry,
I am a women of 35 years old and I am looking forward to visit New York with my best friend Lieve, who is an interior designer, and my mom! She is 60 and has a flowershop in Belgium/Antwerp and still very cool! The 3 of us are from Antwerp but I life since almost 4 years in Spain with my son of 12 and my husband. In Antwerp I was a tourist guide and I had a small restaurant. We would like to stay in Your place because it looks very nice and it seems to be in a very trendy area. looking forward hearing from you, best regards Ilse


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Dr. Andrew R. Gould, MD
- 4004 Dupont Cir Ste 220
Louisville, KY 40207
- 2 Locations
- (502) 893-0159
Dr. Andrew R. Gould graduated from the University of South Carolina School of Medicine in 1995. He works in Louisville, KY and 1 other location and specializes in Otolaryngology and Plastic Surgery - Head & Neck. Dr. Gould is affiliated with Baptist Health Louisville, Flaget Memorial Hospital, Jewish Hospital and Norton Suburban Hospital. He speaks English and Spanish.
Gender: Male
Languages Spoken: English, Spanish
Otolaryngology, Plastic Surgery - Head & Neck
Medical School: University of South Carolina School of Medicine; Graduated 1995
*This physician has multiple office locations. See Office Information for details.


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
39 year old man | Manhattan, NY, USA | Seeking women 28-40 within 3 miles of New York, NY
Active within 1 hour
The first thing I notice is a smile...always...
The first thing I notice is a smile...always (girls who smile always stay beautiful). The most beautiful curve on a girl is her smile (almost). I also have a soft spot for girls with brown eyes. Ideally, I'd like to meet someone who is smart, kind, easy going, confident, witty/sarcastic a plus, well-traveled/cultured and open-minded. It also helps if you live in Manhattan or nearby. I'm not buying an EZ Pass to date you...sorry! I try to be present and mindful as much as I can on a daily basis. I'm easy going, don't take myself seriously and i don't sweat the small stuff (you can tell the size of a man by the size of things that make him mad). I tend to be an introvert at first but do open up as I get more comfortable. I listen to music all the time (sad music makes the best music), at work, at gym (not sad music), at home, running errands. So, having an appreciation for good music is a huge plus. Many favorites but a few are: "Holocene" by Bon Iver; "All My Days" by Alexi Murdoch and "Say Hello Waive Goodbye" by David Gray. Ben Howard, Citizen Cope, James Vincent McMorrow & Chet Faker are also some of my favorites. Favorite sports team: Arsenal (football club in North London). Maybe catch a game together in London. I also volunteer as a mentor/coach with Soccer Without Borders. Added perk: free tennis lesson(s). And, please don't wink, like, write, nod, twit or twerk if you don't have a pic or if your profile is hidden. I'm sure your personality is awesome but I still like to see who I'm talking to and I don't want to end up on the next episode of catfish.
Camping , Coffee and conversation , Dining out , Museums and art , Music and concerts , Nightclubs/Dancing , Performing arts , Playing sports , Political interests , Travel/Sightseeing , Volunteering , Watching sports , Wine tasting
Cycling, Football, Other types of exercise, Skiing, Soccer, Swimming, Tennis / Racquet sports, Walking / Hiking, Weights / Machines, Yoga
I exercise 3-4 times per week.
I have Dogs
I like cats enough not to kill them
West Village, Bowery Ballroom, Joe's Pub, city winery, Eataly, Freeman's, sushi of Gari, Peasant, The Vikings, National Geographic, History Channel, Vail, Turks, St. Martin, Paris, Buzios, Tulum, Tamarindo, Arsenal Thailand, Tuscany
Wake Forest University, Winston-Salem, NC
Arsenal, music, good coffee, dried mangoes, ice cream, donuts (don't judge), comfortable t-shirts, jeans, fitted suits, cool kicks, Saturday mornings with my nephews & niece, random acts of kindness, beach vacations in the winter, soccer, tennis
Working out, yoga, hiking, soccer/tennis, non-fiction books, riding my bike, art exhibits, weekend getaways, Saturday morning breakfast w/my little people (2 nephews and niece), unsolicited kindness.
The Spirit of Healing
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|5'11" (180cms)
|5'3" (160cms) to 5'10" (177cms)
|Body type:
|Athletic and toned
|Slender, Curvy, Athletic and toned
|No preference
|Dark brown
|Black, Light brown, Dark brown, Blonde, Dark blonde
|No Way
|No preference
|Social Drinker
|Never, Social Drinker, Moderately
|No preference
IP lawyer (mainly in fashion)
|I'll tell you later
|No preference
|Never Married
|Never Married, Widow / Widower, Currently Separated, Divorced
|Have kids:
|Wants kids:
|Middle Eastern, White / Caucasian, Other
|Latino / Hispanic, Middle Eastern, Native American, Pacific Islander, White / Caucasian, Other
Born in Iran (Don't be scared, you only have to walk behind me every other day....... spent a few years in Europe before moving to the land of opportunities, dreams and 12-hour days.
|Spiritual but not religious
|Agnostic, Atheist, Buddhist / Taoist, Christian / Catholic, Christian / Protestant, Muslim / Islam, Spiritual but not religious, Other, Christian / Other
Good Thoughts Good Words. Good Deeds..Just be a good person without being a slave to rules and restrictions...Life is too short.
|English, French, Other
|Graduate degree
|Bachelors degree, Graduate degree, PhD / Post Doctoral


text:
Now Showing
This summer is sure to be one filled with many beach days, but when the temperature is just a little too hot or the beach a little too busy, watching a movie might be the perfect solution.
With all the films premiering this summer, everyone must be interested in at least one.
Directed by Ridley Scott, sci-fi thriller Prometheus is expected to be one of this summer's big movies.
Are you a newspaper subscriber but you don't have a Digital Access account yet? https://secure.forumcomm.com/?publisher_ID=40&event=subscriber/lookup.
You will need your subscription account number and phone number. Not sure if you have an account? Email us at email@example.com and we can help you.


text:
Presented by:
Thank you for celebratating philanthropy and those who make a difference in our community!
Awards were presented for:
Outstanding Fundraising Professional
Jeff Lorber at UIS
Outstanding Philanthropic Corporation
Springfield Clinic
Outstanding Philanthropist
The Becker Family
Outstanding Civic or Service Organization
Rotary Club of Springfield Sunrise
Outstanding Volunteer Fundraiser
The Brown Family
AFP Quick Poll
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Mar 12 - Director of Annual Giving
MacMurray College was founded in 1846, has a strong history of undergraduate education, and is located in the affordable, safe, and stimulating city of Jacksonville, Illinois. Our mission is "to transform the lives of our students by providing an exceptional, liberal arts general education and courses of study that prepare graduates for professional careers, further education, and other challenges facing the human community in the 21st century." ... more >
Mar 11 - MAJOR AND PLANNED GIVING OFFICER
MacMurray College was founded in 1846, has a strong history of undergraduate education, and is located in the affordable, safe, and stimulating city of Jacksonville, Illinois. Our mission is "to transform the lives of our students by providing an exceptional, liberal arts general education and courses of study that prepare graduates for professional careers, further education, and other challenges facing the human community in the 21st century." ... more >
Mar 11 - Specialist, Relay for Life (fundraiser)
Executes a portfolio of Relay For Life events, with accountability for significant income targets, as well as event-related mission and advocacy activities. Ensures goal achievement through the effective leadership, engagement, empowerment, and mobilization of event volunteers. How to Apply: Please visit www.cancer.org/careers. Search Location: Springfield, IL ... more >
Mar 11 - Director of Development (2 positions)
: Representative who plans strategies and actively seeks major gifts for the benefit of the University of Illinois at Springfield (UIS). The position's emphasis is on identification, cultivation, solicitation, and stewardship of major gift donors and prospects. The individual is expected to develop and manage a caseload of approximately 150 prospects and have 15 or more face-to-face donor visits or other significant donor contacts per month. ... more >
Feb 23 - Director of Development (2 Positions) University of Illinois Springfield
Full-time, Academic Professional positions in the Office of Development, the fundraising arm of the University of Illinois Springfield. ... more >
Mar 25 - AFP, Relationship Science Announce Strategic Partnership
The Association of Fundraising Professionals (AFP), the largest community of fundraising professionals in the world, has announced a new strategic partnership with Relationship Science (RelSci), a technology solutions company that enables organizations to strategically leverage the collective connections of employees, executives, and board members in order to shorten fundraising cycles and identify high-impact donors. ... more >
Mar 25 - AFP Texas Lobbying Event a Huge Success
AFP chapters from across Texas came together in Austin at the State Capitol building in early March to raise awareness of philanthropic and fundraising policy issues at the second AFP Texas Legislative Action Day. ... more >
Mar 25 - Top Ten (Week of March 23, 2015): The Lure of Poverty Porn for Nonprofits
(March 23, 2014) Learn about this topic and more in this week's top ten, including what nonprofits can learn from Uber and AirBnb; the psychology of distractions; why you should crowdfund for your nonprofit; 10 insanely useful (and free!) Twitter tools for nonprofits; five ways to sabotage your call-to-action; and more! ... more >
Mar 25 - Bernard Ross Reveals the Next Big Thing in Fundraising!
(March 25, 2015) Have you ever wondered what your donors are thinking? Life would be so much simpler if you could read their minds. Now, we're actually a step closer to knowing. To understand what your donors are thinking, you first need to understand how they think. That's where veteran consultant and author Bernard Ross, director of The Management Centre, and fundraising consultant Alan R. Hutson, Jr., principal and Managing Partner of The Monument Group, can help. ... more >
Mar 25 - Discover What Company CSR Executives are Thinking
(March 25, 2015) So, what are considered best practices in CSR? Does a CSR program bolster a company's reputation? How does the company know one way or the other? How do companies keep on top of tracking, measuring and reporting impact? What is the future of CSR? What will the implications be for both corporations and the nonprofit sector? These, and other questions, will be addressed in the session "Understanding CSR from the Corporate Perspective" at the AFP International Fundraising Conference (Baltimore, March 29-31, 2015). ... more >
April 15, 2015 - AFP Meeting
May 20, 2015 - AFP Meeting
September 16, 2015 - AFP Conference
October 21, 2015 - AFP Meeting
November 18, 2015 - AFP Meeting


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New York: Actor Vincent Gallo has slammed reports that he coerced actress Chloe Sevigny to do the notorious oral sex scene with him in his 2003 movie 'The Brown Bunny' because they were dating.
"Chloe and I were never boyfriend and girlfriend. In 1995 we made out once in Paris," the New York Post quoted him as saying.
"I feel Chloe has suggested we were boyfriend and girlfriend to lessen the boldness of her appearance . . . and to portray herself as a devoted girlfriend and victim rather than a great radical performer.
"Chloe brilliantly understood that the media would persist in thinking that she did it out of loyalty to me," he added.


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Personalized All the Reasons Chalkboard
Personalized All Because . . . Canvas
Personalized Photo Pillow
Personalized Like Eggs & Bacon Canvas Wall Art Art
Personalized Fairytale Moment Canvas Wall Art
Personalized Love You to the Moon and Back Pillow
Personalized From the Heart Framed Print
Personalized Timeless Treasure Watch Box
Personalized 20 Watch Wood Box
Personalized Now and Then Double Frame
Personalized Always Kiss Me Goodnight Canvas Wall Art
Personalized You Are My Happily Ever After Canvas Wall Art
Personalized Our Family Circle Canvas Wall Art
Personalized Gave Me You Plaque
Personalized The Day We Became & Canvas Wall Art in Red
Personalized Love Birds Half Round Doormat
Personalized You 'n Me Watch Box
Personalized Happiness in Life Wall Plaque
Personalized The Day We Became Pillow
Personalized Photo Tile Box
Personalized Monogram Wood Plaque
Personalized Beside You Canvas Wall Art
Personalized "Remember When" Family Pillow
Personalized Together is Our Favorite Place Pillow


text:
Answers (3)
7 May 2013
Probably not - the combination could cause unusual bleeding. You should check with your doctor. You may be ok to take Motrin but at a lower dose.
It is safe for you to take acetaminophen.
12 May 2013
My doctor advises using only acetaminophen (Tylonal) for pain and fever while taking Plavix. The side effects of Plavix with other medications has to be closely monitored. I was on Plavix 15 years ago and developed stomach bleeding. It was rather unpleasant. My stool was jet black and had tar like consistency. After week in the hospital and a unit of blood, I was back on my feet. They took me off the Plavix. This past week my stents closed up and had to be replaced. I am back on Plavix and my heart surgeon will not even consider using anything else. I have read reports in the past that Plavix is no better than aspirin but who knows what is true anymore with these meds? The drug companies promote their potions and the holistic folks will say the companies are lying etc.
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Since taking plavix my toungue seems to be swollen. I also will have sores and it feels as if ther?
something hung between my teeth. this is very annoying and i will try to remove without sucess. is this problem due to plavix?
1 answer • 23 Apr 2010
Is it ok to mix tylenol with motrin for pain relief? How about Aleve with motrin?
I have a severe toothache. X-rays at dentist today showed infection. Clindamycin was prescribed as well as 800mg Motrin. I have been mixing Tylenol ...
4 answers • 17 Aug 2010
Why does motrin 800mg work better than taking 4 200mg otc?
it seems to me when i take 1 motrin 800mg it works better than taking 4 of the 200mg otc. except for milligrams is there different properties in them
1 answer • 26 Feb 2011
When you stop taking plavix for a procedure, can swelling in the ankles occur?
my mother stopped taking plavix for a tooth extraction. five days after not taking the plavix she started to have swelling in the ankles, and ...
1 answer • 1 Sep 2013
Can I take one motrin ib 200mg ? daily taking 1-asprin 325mg?
toprol xl 50mg & 1 clopidogrel 75mg.
1 answer • 29 Dec 2013


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Social media
Now that social media services have burrowed into federal IT operations, agencies are beginning to face the challenge of honing effective strategies for their internal and external users.
Public-facing social media services need a lot of thought and care so they do not become unruly and aimless, according to federal experts who spoke at an event sponsored by AFCEA's Bethesda chapter.
In other words, "social media is free like a puppy is free," said Ryan Koch, deputy director of the State Department's Office of Innovative Engagement.
Agencies must thoroughly understand not only what they want to get out of social media, but what their customers are looking for from Twitter feeds, Facebook pages, Tumblr and Instagram accounts and other social media services.
Scott Horvath, Web and social media chief in the Office of Communications and Publishing at the U.S. Geological Survey, said most federal agencies have a social media presence and are now in the process of developing a better sense of how to shape that presence into something customers can use.
All the panelists recommended that agencies review the General Services Administration's HowTo.gov online guide to using social media at a higher level. Tammi Marcoullier, program manager of GSA's award-winning Challenge.gov site, said federal IT managers should not be afraid to jump into social media services and should keep experimenting even if they fail at first.
Horvath and Amanda Nguyen, director of Web communications at the Agriculture Department, said federal agencies have unique opportunities to engage with the public by combining their vast troves of data with social media capabilities.
That approach can result in useful public input. For example, Horvath said USGS monitors Twitter for mention of the word "earthquake" in a variety of languages. A proliferation of the word can mean a seismic event is imminent and give the agency a heads-up on a possible quake. The information might arrive before the agency's worldwide array of seismic monitors senses anything, which gives the agency the opportunity to retweet the information while combining it with seismic data to give the public a fuller picture of the event.
Koch said State faces the challenge of tailoring its social media activities to users worldwide, including those who live in places that might not have broadband access. Officials must consider the countries their 700,000 Twitter followers live in and the demographics of the specific users they are trying to reach.
"You have to tailor to match your audience," Koch said. "You may not talk high-level trade but [instead] narrow it down to copyright infringement" depending on the audience.
That level of detail might be lost on agency leaders who have been interested only in the volume of Twitter traffic or Web page hits, Nguyen said. But as they begin to ask deeper questions about social media analytics, they will start to find smarter uses for the platforms.
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text: Ideal suburi away from dojo - Kendo World Forums Login or Sign Up Log in with Facebook Search in titles only Search in Training only Advanced Search Search SECTIONS Home Magazines Books Forums Mag-TOC YouTube Channel FAQ Groups Today's Posts Mark Channels Read Member List Home Forum Kendo Training If this is your first visit, be sure to check out the FAQ by clicking the link above. You may have to register before you can post: click the register link above to proceed. To start viewing messages, select the forum that you want to visit from the selection below. Announcement Announcement Module Collapse No announcement yet. Ideal suburi away from dojo Page Title Module Move Remove Collapse X Conversation Detail Module Collapse Posts Latest Activity Search Page of 1 Filter Time All Time Today Last Week Last Month Show All Discussions only Photos only Videos only Links only Polls only Filtered by: Clear All new posts regularyojimbo Member Join Date: Feb 2004 Posts: 94 #1 Ideal suburi away from dojo 10th July 2004, 05:24 AM Would be interested to hear people's idea of ideal suburi away from the dojo, using bokken or shinai and the type of suburi(s) with explanations. With thanks. Tags: None Swissv2 れっとうせい Join Date: Jun 2004 Posts: 305 Wonder what Yoda would be like in Kendo? #2 10th July 2004, 04:02 PM The right form (check for posts about this) and you shuld be fine. Bokken is preferable. But use a shinai every now and then, it will feel light as a feather after you use bokken (depending on weight difference) Comment Cancel Post New Style Default vb5 Style vB4 Default Style (vB4 Style - Incompatible) - KW Old Style (vB4 Style - Incompatible) -- KW Standard Style (vB4 Style - Incompatible) English (US) Japanese Go to... Help Contact Us Help Contact Us Powered by vBulletin™ Version 5.1.0 Copyright © 2015 vBulletin Solutions, Inc. All rights reserved. Content © Bunkasha International K.K. Working... Yes No OK OK Cancel X


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Slideshow: Mississippi Street Fair
Posted Jul 11, 2011, 12:00pm
It was hot on Saturday. Melting gum on the sidewalk, shirtless-guy-who-probably-shouldn't-be-going-shirtless kind of hot. It was also the 10th annual Mississippi Street Fair. And if you happened to shuffle your way down Mississippi Avenue between Fremont and Skidmore streets, weaving through the train of baby strollers (or possibly pushing one yourself) and dodging the adorable-yet-slobbery pooches, well, Portland Monthly was right there with you. Big Dawn Blackwood ...
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Phenom 300 getting instructions from the tower before she crosses runway 25 and takes off 21L. As always "Thanks" to the staff at KLUK.
Sharp looking RV-8 sitting in the shade waiting for tower instruction for takeoff on runway 21L.
Early morning landing 21L. Sharp looking and compact.
Ultimate Air Shuttle early morning departure off 3R to Chicago.
Sharp looking Cirrus ready to taxi out to 21L for takeoff. Registered in Iowa and manufactured 2006.
Proctor & Gamble Gulfstream on final for 21L.
Tight shot of the Channel 9 news chopper.
Is this a sharp looking biz jet or what? Manufacture date of 1982, flying out of Florida and looking brand new.
Channel 9 News chopper "On Your Side" heading out of Lunken Airport for traffic reports.
Just in off 21L and passing the old barn yard hangar area. Thanks Charlie.
Just in off 21L and passing the old barn yard hangar area. Thanks Charlie.
Nice close shot of the "Aluminum Overcast" as she rolls out from the old control tower area and taking about a dozen folks for a ride of their life on the big WWII B-17.
Great place to watch approaching aircraft into runway 3R right off Kellogg Ave. where they extended the bike trail ( 39.086991,-84.421583 ). Here's the B-17 (Aluminum Overcast) over the trail with... (more)
Framing the big B-17 at Lunken Aviation Days is a 1946 Ercoupe with neat paint. Check this; 85HP, 105MPH cruising speed, 1400 Lbs. weight, 30 Ft. wing span, 20 Ft. long and no rudder pedals. Built... (more)
Loaded up a group of folks at Landmark North and headed out to runway 3R for takeoff. Don't see too many black tails. That's a Netjets in the background.


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A burrito question
Hi - I have a possibly dumb question about burrito method cuttings. I took a bunch 2 weeks ago, and some have formed nice callouses, but no roots. Would it be best to pot these up now, or should I leave them in their wrappings for another week in the hopes that they'll form roots before potting them up? I have read all the threads I could find on this method, but am unsure on this point.
Hopefully Kim will add, but just so you get a quick answer, I would try and pot and see what happens. My attempt at burrito got me good callouses but no actual white roots, however most still took once planted.
What I understood from his demo on the 21st was that if nothing happened in 2 weeks, that more time was not going to make a difference because the cane would have used up its stored energy by the two weeks.
Here is a link that might be useful: Roseseek on Burrito
Thanks so much, Kippy, this was just the kind of information I was hoping for! Its really useful to know that the 2 week period is the amount of time during which the cane has enough stored energy. I guess that suggests that the ones that have not calloused probably won't; but since with them I really have nothing to lose, I guess I'll just leave them for a while longer, just in case.
Thanks, Kippy. Hi Frances! I just removed a wrap after two weeks which hadn't callused, either. I planted all ten of the cuttings anyway as some MAY root. I had no control over their condition as they were sent to me by a friend out of state. I'd had a few callus in late October into November I used for a demo, then the next batch didn't. I'm waiting until next month (late January) before I take any more to wrap as that has been when they've been most productive here. Kim
That should be OK, Kitty (hopefully!). I had callusing success back in November for a demo I did. The next month, they didn't work either for the demo that month, nor for the cuttings I received from a friend. I wrapped many from my own garden this week and plan on doing more from a friend's garden in the next week or so. Each year (and rose) is different, so it just takes experimentation. If you want to wrap some now to test the success, you might find you CAN do it now (or not). I wouldn't put all of them in right now in case something isn't right, but if this batch calluses in the two week period, it should be safe to do the rest once you've seen they're working. Good luck! Kim
I'd feared it had been too warm with our lack of any real "winter" for the wraps to callus, but they've produced like troupers. Then, I worried with low humidity, brilliant sun, no rain and days in the seventies to mid eighties, the planted cuttings would fail. Nope! Several have roots at the soil ball bottoms after two weeks planted. The only batch which hasn't callused as hoped has been Pink Clouds (wrapped for root stock), but all the others are producing better than expected, including VI Fortuniana. There are three new blog posts with photos and explanations at the link below. Kim
Here is a link that might be useful: 2014 Burrito Method results to date


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Organizing spices
I am new to the forum. I had posted a couple of days ago but cannot find my post hence the repost. :-(
I use a lot of spices in my cooking. I buy them from the international store in small plastic packs and then store them in glass or plastic jars. I would like them to be in identical glass or plastic jars and would like to stack them. I need the capacity to be at least 7 oz and I need the bottles to be airtight. Any ideas on where to look? Any brand recommendations?
Thanks in advance!
I was lucky to find these jars (they were full of spices) at a grocery store in Italy. They were 50 cents each. I emptied the spices before packing them thinking that security check might think they were drugs. LOL
The ones on the right were on clearance after Christmas and had green and red sugar. I love repurposing bottles and jars.
My response wasn't very helpful as I forgot to add a source for spice bottles.
Here is a link that might be useful: Bottles Online
Okay, sorry if this is a dumb idea, but how about canning jars? The small ones are 8 oz (I've seen 4oz too), and they stack nicely.
One place that comes to mind to look for jars/bottle/containers, is a place called McMasterCarr. You can find them online. They are a large wholesale supplier, but sell to the public. They carry a good number of restaurant and lab supplies.
Here is another on-line source.
Here is a link that might be useful: Specialty Bottle
Here is a link to pics of drawers with the specialty bottle clear topped jars. I love the idea.
Here is a link that might be useful: spice drawers


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May 24, 2011; Minneapolis, MN, USA; Minnesota Twins outfielder Ben Revere (11) against the Seattle Mariners at Target Field. The Twins defeated the Mariners 3-2. Brace Hemmelgarn-US PRESSWIRE
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Graphic Designer
Closed - This job posting has been filled and work has been completed.
Job Description
Hi all we'd like to have an extra hand to design for our websites...Mostly logos banners, sliders etc.
If you've got some extra hours and want to make some cash doing what you're great at and love doing, then come on in. We start immediately.
I usually provide illustrations for you to follow so as to avoid misunderstandings. So its quite important that things that are requested to be blue are not delivered in green.