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PS4 News - Latest PlayStation 4 and PS3 News
European PSN Store and PS Plus Updates for September 7, 2011
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186w ago
- SCEE's
Ross McGrath
of the PlayStation Store Team is here with this weeks European PSN Game Store and PS Plus updates today, as follows:
Hi everyone - a long intro from me today, lots of housekeeping and new things to tell you about, but let's kick off with the third title in the PLAY promotion - Bloodrayne: Betrayal launches today.
The original video game femme fatale, Rayne, has returned to battle and feed in a brand new hack 'n slash adventure. Slay ghastly enemies as Rayne, the sexy dhampir with superhuman strength, speed, and agility, recruited by the vampire hunting Brimstone Society for one last mission.
The target is a lavish, yet sinister ball in a secluded castle with plenty of dark and gruesome surprises. With the help of a mysterious friend, and members of Brimstone, Rayne must infiltrate the manor, take out the fiendish horde, and stop an evil master plot once and for all.
Bloodrayne: Betrayal is available this week in all locales except for New Zealand (Majesco are finalising the OFLC rating for this title in NZ and we will be able to bring it to you next week).
Also, Italian consumers can this week begin to take part in the PLAY promotion - Street Fighter III: Third Strike, The Baconing and Bloodrayne: Betrayal are all launching on the Italian Store and you can now purchase any of these titles and if you purchase all four, become eligible to receive your reward copy of PAYDAY: The Heist.
SNK bring us a whole host of new minis this week as well - with more on the way. These classic titles from the arcade era are now available for you to enjoy either on PS3 or PSP - Guerilla Wars is free for Plus and all the other titles (and those still on the way) will be available at 20% off for our subscribers too.
Also this week we have plenty of other choice morsels for you, including the slightly delayed God of War Collection: Volume II demo - Kratos' PSP adventures have been upscaled and brought to life on PS3 for the first time. The eu.playstation.com team has put together a demo guide for you to check out, so make sure to head on over there to get the inside track.
You've probably already seen Andy's post about the second round of PSP titles for the big PSP Power Sale, including the launch offer for Persona 3: Portable. Disney were kind enough to include Lego Pirates of the Caribbean in the sale too, to commemorate the launch of Pirates of the Caribbean 4: On Stranger Tides on the Video Store on 12th September.
Another quick note regarding Automatic Update - the SCEI team are aware of the issues that you guys have been having since the firmware update and there will be an interim update to fix the issue coming later in September - please keep a look out for this and when I can update you with an exact date we'll let you know.
That's all from me for now - check out the content lists for Plus and the Store Update below and both Andy and myself will be around for comments both today and tomorrow - unfortunately my workload has yet to let up and keeping you guys informed is going to take the both of us!
PlayStation Store (via PS3, PSP and Media Go)
PlayStation Plus Content
Plants vs Zombies - 100% off
Ratchet and Clank: 'All 4 One' Open Beta (Exclusive)
Jellypop (minis) - 100% off
Kaleidoscope (minis) - 100% off
Resident Evil: Director's Cut - 100% off
(excludes Germany and New Zealand)
Street Fighter Alpha 2 (Psone) - 100% off
(Germany and New Zealand only)
Bloodrayne: Betrayal - 20% off
Explodemon! - 50% off
Guerilla War SNK mini - 100% off
20% off all SNK minis:
Athena - 20% off
HAL 21 - 20% off
Marvin's Maze - 20% off
Ozma Wars - 20% off
Sasuke Vs. Commander - 20% off
Vanguard - 20% off
The Impossible Game (minis) - 25% off
100% off all Resident Evil: Operation Racoon City Avatars
Beltway Avatar
Bertha Avatar
Four Eyes Avatar
Licker Avatar
Lupo Avatar
Spectre Avatar
Tyrant Avatar
Vector Avatar
Zombie Avatar
Resident Evil: Operation Racoon City Dynamic Theme (Exclusive)
Assassin's Creed: Revelations Dynamic Theme (Exclusive)
Watch out for additional offers in PlayStation Plus throughout September, including more discounts on catalogue content, more new Plus exclusive avatars, and launch day discounts on Capcom's 'Resident Evil 4′, 'Resident Evil: Code Veronica' and Ubisoft's 'From Dust'
Special Offer Selection
For all this information go and visit Andy Stewart's new Special Offers Update.
Trial & Unlock (PS3)
Bloodrayne: Betrayal (£9.99/€12.99)
- The Trial and Unlock have been bundled together for Germany
- This product is free for those who have pre-ordered as part of PLAY
Rating: PEGI 16
Availability: Not available in New Zealand
Red Johnson's Chronicles (£7.99/€9.99)
Rating: PEGI 16
Availability: All Locals
SkyDrift (£11.99/€14.99)
Rating: PEGI 7
Availability: All Locals
Full Games (PSP)
Persona 3 Portable (£23.99/€29.99 during the PSP Power Sale until the 21st September then will change to £27.99/€34.99)
Rating: PEGI 3
Availability: All locales
Cladun X2 (£15.99/€19.99)
Rating: PEGI 3
Availability: Not available in Australia or New Zealand
minis (PS3 and PSP)
The Impossible Game (£1.74/€1.99)
Rating: PEGI 3
Availability: All locales
Athena (£1.99/€2.49)
Rating: PEGI 12
Availability: Not available in Australia or New Zealand
Guerilla War (£1.99/€2.49)
Rating: PEGI 12
Availability: All locales
Hal 21 (£1.99/€2.49)
Rating: PEGI 12
Availability: All locales
Marvin's Maze (£1.99/€2.49)
Rating: PEGI 3
Availability: Not available in Australia or New Zealand
Ozma Wars (£1.99/€2.49)
Rating: PEGI 3
Availability: Not available in Australia or New Zealand
Sasuke vs Commander (£1.99/€2.49)
Rating: PEGI 3
Availability: Not available in Australia or New Zealand
Vanguard (£1.99/€2.49)
Rating: PEGI 3
Availability: Not available in Australia or New Zealand
PSone (PS3/PSP)
Tomb Raider II Starring Lara Croft (£4.79/€5.99)
Rating: PEGI 12
Availability: All Locales
Demos (PS3)
God of War Collection: Volume II Demo
Rating: PEGI 18
Availability: Not available in Germany
Assassin's Creed Revelations Multiplayer BETA
Rating: PEGI 18
Availability: Not available in Germany
Available to play until 11th September
PES 2012 - Pro Evolution Soccer
Rating: PEGI 3
Availability: All locales
Add-On Game Content (PS3)
Battlefield Bad Company 2 - Add-on Bundle (£23.99/€29.99)
(contains the following)
Onslaught Expansion Pack
Vietnam Expansion Pack
Kit Upgrades - Recon
Kit Upgrades - Assault
Kit Upgrades - Engineer
Kit Upgrades - Medic
Kit Shortcuts - Assault
Kit Shortcuts - Engineer
Kit Shortcuts - Medic
Kit Shortcuts - Recon
Rating: PEGI 16
Availability: All Locals
DanceDanceRevolution: New Moves - SuperNOVA Greatest Hits 2 (£2.99/$3.59)
Rating: PEGI 12
Availability: All Locals
Dynasty Warriors: Gundam 3 - Extra set of Mobile Suits 4 (£3.19/€3.99)
Rating: PEGI 12
Availability: Not Available in New Zealand
Champion Jockey: G1 Jockey & Gallop Racer - Music Pack 1: G1 Jockey selection (£0.95/€1.19)
Rating: PEGI 3
Availability: All Locals
EyePet - Lucky Dip Fruit 2 (free)
Rating: PEGI 3
Availability: All Locals
LittleBigPlanet2 - World Peace Day Costume (Sept Seasonal) (free)
Availability: All Locales
Resistance 3 - Network Pass (£7.99/€9.99)
Rating: PEGI 18
Availability: All Locales
Rock Band Network
Eden - TesseracT (£0.99/€1.49)
Expert Mode - Leetstreet Boys (£0.59/€0.79)
Midnight Eyes - Rose of Jericho (£0.59/€0.79)
Morte Et Dabo - Asking Alexandria (£0.99/€1.49)
Piano Sonata No. 11 - Ronda Alla Turca (Mozart) - Thomas Walker (£0.59/€0.79)
Rating: PEGI 12
Availability: Not Available in Croatia, Czech Republic, Greece, India, Israel, Kuwait, Luxembourg, Poland, Russian Federation, Saudi Arabia, Slovenia, South Africa, Turkey, Ukraine, UAE
Rock Band 3
Unbelievable - EMF (£0.99/€1.49)
Cheap Trick Pack 01 (£2.49/€3.99) (contains the following songs also available separately)
Dream Police - Cheap Trick (£0.99/€1.49)
I Want You To Want Me (Live) - Cheap Trick (£0.99/€1.49)
Surrender (Live) - Cheap Trick (£0.99/€1.49)
Rating: PEGI 12
Availability: Not Available in Croatia, Czech Republic, Greece, India, Israel, Kuwait, Luxembourg, Poland, Russian Federation, Saudi Arabia, Slovenia, South Africa, Turkey, Ukraine, UAE
Street Fighter III: Third Strike - Character Color Pack 1 (£1.59/€1.99)
Rating: PEGI 12
Availability: All Locales
Resident Evil: Operation Raccoon City
Beltway Avatar
Bertha Avatar
Four Eyes Avatar
Licker Avatar
Lupo Avatar
Spectre Avatar
Vector Avatar
Zombie Avatar
All available @ £0.20/€0.25 each
Themes (PS3)
StarHawk Theme (free)
Dynamic Themes (PS3):
Dynamic 3D Blue Plasma (£1.59/€1.99)
Bacon (£1.59/€1.99)
Light Effects Theme (£1.59/€1.99)
Ants Ruin Picnics! (£1.59/€1.99)
Bald Eagle Theme (£1.59/€1.99)
Lion (£1.59/€1.99)
Gates Of Olympus Theme (£1.59/€1.99)
Water Drops Theme (£1.59/€1.99)
City At Dawn Theme (£1.59/€1.99)
Videos (PS3)
Deus Ex: Human Revolution Launch Trailer
Anarchy Reigns Gamescom Trailer
SkyDrift Debut Trailer
Sonic Generations Gamescom Trailer
Spelunker HD Trailer Video
Tour De France - Trailer
UFC Undisputed 3 Combat Trailer
Warhammer 40,000: Space Marine Narrative Trailer
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Public Affairs & Government Relations
NAMM stands for its Members every day by providing ongoing representation in Washington, D.C. on issues that impact the music products industry, including:
- Music education and education reform legislation and funding
- Small business health insurance legislation
- Estate tax reform
- Import/export laws
- E-commerce tax reform
Advancing Music: Public Policy, Music Education and Music Making
A blog by Mary Luehrsen, Director of Public Affairs & Government Relations
Don't Miss These Upcoming Public Affairs Events
IP Academy
NAMM Members have the opportunity to participate in a free two-day, Intellectual Property (IP) Academy April 8 and 9, 2015, at the Global Intellectual Property Academy (GIPA) of the United States Patent and Trademark Office (USPTO) in Alexandria, Virginia. In its third year, the program is designed to help attendees understand strategies and to protect and manage IP, including patents, trade secrets, trademarks, and copyrights, domestically and abroad. Speakers will include intellectual property experts from the Office of Policy and International Affairs (OPIA), U.S. Patent and Trademark Office, as well as guest speakers from the private sector.
NAMM Advocacy Fly-In 2015
Join other NAMM Members for the 2015 Advocacy Fly-In, May 18 – 21, 2015. The 2015 NAMM Advocacy Fly-In advances NAMM's core advocacy theme: music education and legislation that advances access to music education for all children. All Fly-In delegates will be asked to contact Members of Congress in the weeks prior to May 18 to schedule meetings with Representatives or their staff.
Optional Day of Service, Monday, May 18: the 2015 Advocacy Fly-in will feature an opportunity for NAMM Members to join together for a day of service at a school in Washington DC. NAMM will celebrate and support the music education program in the local area. All NAMM Members participating in music learning activities are encouraged to consider an equipment or materials donation to the school; donations will be coordinated by NAMM Staff regarding shipment and delivery to the school.


text: As a writer for Rock, Paper, Shotgun I am contractually obliged to know absolutely nothing about the series that Capcom are most recently rebooting. Previously consigned purely to the arcades and consoles, protagonist Strider Hiryu and his menagerie of robots was firmly beyond my grasp. I was disallowed any knowledge of his wall-hanging, ninja-running, platform-jumping ways and any investigation into the original late 80s arcade game, which this remake is based on, was redirected to Monkey Island Studies. Thank goodness for other people's nostalgia, as from today the pyjama-clad death machine is available on Steam with a launch trailer below, if you're quick enough.


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text:
A device, system and method for portable fluorescence detection. The portable device of the present invention features a low power light, in which a wavelength range is defined as at least one wavelength of light. The light source is preferably highly energy efficient, such that a majority of the electrical power which is consumed is then converted into transmitted light. The emitted light from the excited fluorophore is then preferably detected with any low cost and low power photodetector. Although optionally a highly sensitive optical detector may be used, preferably fluorescence is detected with any light sensing device, such as a regular photodiode or a CCD (charge-coupled device) sensor for example.
1. A portable device for detection of fluorescence in a sample containing a fluorophore, comprising:
(a) a light source for emitting light for exciting the fluorophore, wherein said light is of a defined wavelength range; and
(b) a photodetector for detecting emitted light from the excited fluorophore.
2. The device of
3. The device of
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15. The device of any of
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26. The device of
27. The device of
28. The device of
29. The device of
30. The device of
31. The device of
32. A system for detection of fluorescence in a sample containing a fluorophore, comprising:
(a) a portable device according to
(b) a computational device for performing a computation.
33. The system of
(a) a peripheral device.
34. The system of
35. The system of
36. A method for detecting fluorescence in a sample containing a fluorophore, comprising:
providing a portable device according to
entering the sample is preferably entered to said portable device;
emitting light by a light source of said portable device, thereby exciting the fluorophore; and
detecting light emitted from the excited fluorophore by a photodetector.
37. The method of
providing a a computational device for performing a computation.
38. The method of
performing one or more computations on a signal obtained from said photodetector.
39. The method of
displaying a result of said one or more computations.
The present invention relates to a novel device, system and method for fluorescence detection, and in particular, to fluorescence detection with an inexpensive, portable device.
Fluorescence is used as a marker for various biological, medical and diagnostic assays. In order to be able to detect fluorescence, and hence to be able to use it as a marker for these assays, a number of components are required. First, a suitable fluorophore must be selected. The fluorophore should be excitable at a wavelength which is suitable for the given application, with a strong signal (emission of light). In addition, the fluorophore should be resistant to photobleaching, for maximum efficiency of detection.
The fluorophore is then excited by the application of light of the appropriate wavelength. For excitation of the fluorescent material, a very strong light source, such as a LASER, xenon- or mercury-arc lamps or high-powered tungsten-halogen bulb (see Table 1 of the appendix section for a complete list of conventional light sources.). For a white light source, such as xenon or halogen, the light has to be accurately filtered to permit only a very narrow bandwidth of wavelengths to pass for excitation of the fluorophore.
In addition, fluorescence detection requires a very sensitive light detection or sensing device, such as a photomultiplier (PMT) tube, avalanche photodiodes or a CCD video camera, in which the CCD element is cooled to reduce electronic noise so that long exposure times can be used to detect the low amount of light emanating from the above fluorescent materials. The light entering the detection device has to be accurately filtered, to fit the fluorescent emission of the fluorophore.
The electronic components which are required for fluorescence detection are currently expensive and large or heavy, consume a significant amount of energy and may require active cooling and complicated control circuits.
One example of a background art system is disclosed in U.S. Pat. No. 6,287,871, in which a laser light source is employed for excitation in order to view reaction lines on a lateral flow immunochromatography device. The disclosed system includes an optical detection system, in which a collection lens focuses light to a detector, such as a CCD (charge-coupled device) camera or a photomultiplier. Each of these components is both expensive and awkward to handle, due to size, weight, sensitivity to movement or a combination. In particular, the disclosed system may be suitable for a stationary fluorescence detection system, but is not suitable for a portable device for fluorescence detection.
Inexpensive, small (�pocket-sized�), portable devices for fluorescence detection would be highly useful in a variety of applications. For example, such devices would be quite useful in the field of medical diagnostics, in which fluorescence based methods could provide sensitive and accurate tests in non-laboratory environments. Non-limiting examples of such environments include the emergency room, the bedside of the patient at home or in the hospital, physician's office, ambulance, battlefields and other treatment areas which may lack ready access to laboratory equipment and assays.
The background art does not teach or suggest a device, system or method for providing portable fluorescence detection. The background art also does not teach or suggest a highly portable, inexpensive yet robust and sensitive device for fluorescence detection.
The present invention overcomes these deficiencies of the background art by providing a device, system and method for portable fluorescence detection. The portable device of the present invention features a low power light source, preferably of a defined wavelength range. By �defined wavelength range�, it is meant that the difference between the minimum and maximum wavelengths included in the range is preferably restricted to those that are close to the excitation (=absorption) maximum of the fluorescent reporter material but are lower than the emission (=fluorescence) maximum of the reporter material. Optionally and preferably, the term �defined wavelength range� may also encompass light emitted from light sources such as lasers, that emit light of a single wavelength. By �low power� it is meant that the power consumption does not exceed about 500 mW, is preferably less than about 200 mW and is more preferably less than about 120 mW.
The emitted light is then preferably detected with any suitable photodetector. Although optionally a highly sensitive optical detector may be used, preferably fluorescence is detected with any regular photodiode, photocell, photoresistor, phototransistor or noncooled CCD (charge-coupled device) sensor.
The method according to the present invention may optionally be implemented with any device and/or system according to the present invention. One exemplary method preferably includes, in a first stage, providing a portable device for detection of fluorescence in a sample containing a fluorophore, the portable device comprising: a light source for emitting light for exciting the fluorophore, wherein the light is of a defined wavelength range; and a photodetector for detecting emitted light from the excited fluorophore. In stage 2, the sample is preferably entered to the portable device. In stage 3, the light source emits light, thereby exciting the fluorophore. In stage 4, the photodetector detects light emitted from the excited fluorophore. Optionally, in stage 5, one or more computations are performed on a signal obtained from the photodetector. Also optionally, in stage 6, the results of the computations are displayed and/or otherwise provided.
The present invention is preferably suitable for the detection and/or viewing of deposits of fluorophores on flat surfaces, such as the surface of lateral flow immunochromatography devices, by employing low-cost and low-power devices.
The device of the present invention is preferably characterized by being an inexpensive, small (�pocket-sized� or hand-held), portable device for fluorescence detection, which may optionally be powered by a battery.
The invention is herein described, by way of example only, with reference to the accompanying drawings, wherein:
The present invention is of a device, system and method for portable fluorescence detection. The portable device of the present invention features a light source of a defined wavelength range, in which a wavelength range is defined as at least one wavelength of light. The light source is preferably a low power light source, such that the light source has low power consumption. Low power consumption preferably enables the light source to be operable from a small battery. The light source is also preferably energy efficient, such that a majority of the electrical power which is consumed is then converted into transmitted light.
The emitted light from the excited fluorophore is preferably filtered to remove the upper wavelengths that correspond to the wavelength of the fluorescence emission. The fluorescence emission is then preferably filtered and detected with any suitable photodetector. Although optionally a highly sensitive optical detector may be used, fluorescence is preferably capable of being detected with any regular photodiode, photocell, photoresistor, phototransistor or a low-cost CCD (charge-coupled device) sensor in order to simplify the system and reduce its cost.
A preferred example of a low power light source of a defined wavelength range is a LED (light emitting diode), operated at low voltage. For the preferred example described herein, the light source is the Nichia Green LED, providing 10 CD at a current of 20 mA and voltage drop of from about 2 to about 3.5 V. However, substantially any suitable LED may optionally be used. For example, optionally an OLED (organic light emitting diode) may be used as a light source for the present invention. Furthermore, substantially any type of light source being characterized by being at least one of low power and/or low cost may optionally be used.
Optionally and more preferably, the LED is characterized by having a luminous intensity from about 1mCD to about 10 CD. Most preferably, the luminous intensity is from about 10 mCD to about 1 CD. The LED, or any other light source that is used in addition to, or in place of, the LED for the present invention, is optionally implemented as a plurality of light sources, and optionally and more preferably, as an array of light sources.
The LED optionally and preferably emits a colored light. By �colored light�, it is meant light having one or more wavelengths that are dominant, in terms of luminous intensity, as compared to the remaining one or more wavelengths present in the light, if any. For a laser light source, for example, typically the emitted light has only one wavelength, such that the light would have a color according to that single wavelength. Other light sources typically emit light of a plurality of wavelengths. If the emitted light does not have one or more dominant wavelengths, then the resultant light appears to be white rather than colored, as for a regular incandescent light bulb, for example. Such white light is not considered to be colored light within the present invention.
For the present invention, optionally and more preferably, the colored light has a color selected from the group consisting of ultraviolet, white, blue, green, yellow-green, yellow, orange, red, and infra-red. It should be noted that for the present invention, the wavelength(s) of the emitted light is not limited to the visible spectrum. It also should be noted that even for light sources emitting non-colored light, the emitted light may also optionally be colored, for example by treating the light source and/or adding a material to the light source, and/or by filtering the emitted light to form colored light.
Optionally and more preferably, the transmitted light from the light source is filtered, more preferably with a wide bandwidth excitation filter. Most preferably, the filter is a low cost gelatin filter. The filter may optionally be used with any type of light source, such as a light source for producing colored light and/or a light source for producing white light. In any case, the filter is preferably selected according to the desired wavelength of light being transmitted, such as for example according to the preferred wavelength or wavelengths at which excitation of the fluorescent material or fluorophore occurs. For the preferred example described herein, the filter is most preferably a Minus-Red Wratten 44A gelatin filter.
The photodetector is preferably of low cost and/or of low sensitivity. Optionally and more preferably, the photodetector may optionally include one or more of any regular photodiode, a photocell, a phototransistor, a noncooled CCD (charge-coupled device), photoresistor, a sensor photodiode, or an array thereof. More preferably, the photodetector includes a photodiode. Alternatively and more preferably, the photodetector includes a CCD. The photodetector preferably has an exposure time in a range of from about {fraction (1/100)} seconds to about 60 seconds. Most preferably, this exposure time is in a range of from about {fraction (1/70)} seconds to about {fraction (1/10)} seconds.
A preferred example of a photodetector is a low cost CCD sensor, such as the Texas Instruments I TC255, operated at 6-bit grayscale color depth. Optionally and more preferably, the emitted light from the fluorophore, which constitutes the fluorescence signal, is filtered with a suitable filter, such that the filter is able to block the excitation wavelengths. For the preferred example described herein, the emitted light is filtered with an IR filter and 590 nm Long Pass filter. Exposure is optionally in a range of from about {fraction (1/100)} seconds to about 1 second, and more preferably in a range of from about {fraction (1/70)} seconds to about {fraction (1/10)} seconds. In the examples described in greater detail below, exposure was found to be sufficient in a range of from about {fraction (1/60)} to about {fraction (1/30)} seconds. In case higher sensitivity is required and a CCD is employed as the sensing element, exposure time can be increased to the seconds time range.
Optionally and preferably, the fluorophore which is used with the device according to the present invention is a high efficiency fluorophore, which is resistant to photobleaching. More preferably, the fluorophore emits light toward or in a near red and infra-red range (about 600 nm and above), which is the most efficient range of wavelengths for detection by CCD and photodiode sensors, and also for generation of light by LEDs. Preferably, the device includes a filter for filtering emitted light from the excited fluorophore. The filter is preferably selected according to a wavelength or wavelengths of emitted light from the excited fluorophore. One preferred but non-limiting example of such a fluorophore is Alexa 594, available from Molecular Probes, Inc., Eugene, Oreg. 97402-9165, USA. Preferably, the emitted light from the excited fluorophore is filtered with a 590 nm Long Pass filter for this non-limiting example.
The preferred but exemplary combination of excitation and detection devices, described above, was successfully employed with Alexa 594 labeled streptavidin (Molecular Probes, Inc., USA) in the detection of per-sero-conversion HIV positive specimens within 5 minutes of incubation. Such specimens have previously required a high sensitivity ELISA with 4 hours of incubation time. The device of the present invention was able to detect such positive specimens within minutes, in a highly accurate, sensitive manner.
Referring now to the drawings,
The excited fluorophore then emits emitted light 115. Emitted light 115 from the excited fluorophore is preferably filtered through an emission filter 170, and is preferably detected with a single photodetector 180 or an array thereof (not shown). Preferably, photodetector 180 includes any one or more of any regular photodiode, photoresistor, phototransistor, photocells or CCD (charge-coupled device) sensor (preferably a non-cooled CCD), or an array thereof, as described previously.
Photodetector 180 is optionally and preferably connected to a computational device 190 for analyzing the received signal. Computational device 190 may optionally be any type of device that is capable of performing the necessary computations, including but not limited to, a computer, a portable computer, a hand-held computer, a Personal Digital Assistant (PDA), a cellular telephone, or wearable computer, a paging device, or any other suitable device and is optionally a microprocessor-based circuit or device.
The results of the computation can then optionally be displayed on a suitable display device 192, and/or printed through a printer 194, and/or transmitted to a remote location through a connector to a wired 196 or wireless network, wherein a network may optionally include a telephonic system. The results may also optionally be stored, transmitted, displayed and/or manipulated as desired.
A system 185 may optionally and preferably include a combination of device 100 according to the present invention and computational device 190 and/or any peripheral device, including but not limited to, display device 192, and/or printer 194, and/or wired 196 or wireless network. The term �combination� includes but is not limited to, any one or more of in communication with, connected to and/or housed with. System 185 may also optionally, additionally or alternatively, include a lateral flow immunochromatography device for holding the sample and optionally one or more reagents.
The device according to the present invention is preferably capable of detecting, imaging, capturing or otherwise sensing a fluorescent signal that is emitted from an excited fluorophore. As described above, the device is preferably capable of performing this task with a low cost and/or low power light source, and a low cost and/or low sensitivity photodetector. Non-limiting examples of such a light source and a photodetector is a LED and a non-cooled CCD camera, respectively. This Example describes the capture of an image of test results obtained with these non-limiting examples, with a sample containing a fluorophore.
The following was performed for obtaining the image of
Next, the HIV antigen capture line mix was prepared as follows. The following HIV derived recombinant proteins were diluted into a solution of 0.02M carbonate buffer, pH 9.6, 2% sugar (glucose) and 0.25M Urea: Dev-1 recombinant gp41 and C-terminus of gp 120 in Urea buffer obtained from Cytolab, Rehovoth, Israel; Env-1 recombinant gp41 and gp120, Diaproph, Kiev, Ukraine; Recombinant Gag-120, Diaproph, Kiev, Ukraine; and Recombinant gp 36, Standard Diagnostics, Inc.,Kyonggi-do, S Korea.
The mixture was mixed thoroughly for 15 minutes immediately before being sprayed on the nitrocellulose sheet, employing a BioDot robotic XYZ dispensing instrument equipped with a BioJet dispense head. The mixture was dispensed at a rate of 1.0 μL/cm.
Next, Protein A mix for the control line was prepared as follows. Lyophilized Protein A (Zymed, South San Francisco, Calif., USA) was dissolved to a final concentration of 250 μg/mL in Phosphate Buffered Saline pH 7.5, containing 0.1% w/v sodium azide and was used after a 10-minute mixing. It was sprayed on the nitrocellulose sheets as detailed above, at a distance of 7 mm from the HIV antigen capture line as described above. The nitrocellulose strips were then incubated at 60� C. for 15 minutes and dry stored at 15% humidity.
Next, the conjugate pad was prepared as follows. The conjugate pad (polyester 10.3 mm, grade 2033) was pre-treated with sodium phosphate buffer, containing 0.5% BSA, 0.1% Triton-X-100, and 0.5% polyvinyl alcohol (PVA). Strips were immersed in the buffer solution for 2 hours, and then were blotted on a Whatman filter paper to remove fluid excess, and dried overnight at room temperature.
The above antigen dispensed sheets were fitted with an absorbent pad (GB 003 Gel Blotting Paper from Schleicher & Schuell), sample pad (2002 from Schleicher & Schuell) and conjugate pad (see above) as described in the standard literature (Carlberg, D. L. �Lateral Flow Assays: Designing for Automation�, IVD Technology Magazine, May 99 http://www.devicelink.com/ivdt/archive/99/05/001.html, �A Short Guide�Developing Immunochromatographic Test Strips�, Millipore Corp., 1996, Bedford, Ma., USA, Weiss, A., �Concurrent engineering for lateral-flow diagnostics�, IVD Technology 5, No. 7 (1999): 48-57, http://www.devicelink.com/ivdt/archive/99/11/009.html) and cut into 4 mm wide strips.
The final preparation of Antigen Loaded HIV Antibody Test Strips was performed as follows. Protein A tagged with Alexa 594 fluorophore (Molecular Probes, Inc., Eugene, Oreg., USA) was diluted to a final concentration of 83 μg/mL in 0.1M phosphate buffer pH 7.2 containing sugar. Fifteen μL of this protein A solution was dispensed onto the conjugate pad of each of the strips obtained in Example 5 and dried at 37� C. for 2 hours.
The nitrocellulose portion of each test strip was blocked with 20 μL of Phosphate Buffered Saline with detergent and then dried at 37� C. for 2 hours.
Serum specimens were tested for HIV antibody with the test strips as follows. Serum samples were diluted 1:40 in Phosphate Buffered Saline with non-ionic detergent, and a volume of 100 μL was loaded on the sample pad of the test strip and incubated for 10 minutes at room temperature The fluorescence pattern was recorded with the following components.
Excitation Light Source: 6 Nichia Green LEDs (500 mCD each at 20 mA, 3.5V for each light source)
Excitation Filter: Minus-Red Wratten 44A Filter
Emission Detection Camera: unfocused Connectix QuickCam, a 6-bit grayscale Web-Camera (containing the TI TC255 CCD sensor) fitted with a 30 mm diameter, 50 mm focal length piano-convex lens (K32-484, Edmund Industrial Optics, Barrington, N.J., USA) for close up optical correction and a 590 nm Long Pass filter (51311, Oriel Corp., Stratford, Conn., USA).
The device according to the present invention was further tested in this Example, to determine whether it could distinguish between positive and negative HIV specimens.
A fluorescence test system was constructed from 5 surface mounted yellow LEDs, which served as the excitation light source, and 5 photodiodes for detecting the emitted fluorescence. A red glass filter was installed in front of the photodiodes to block excitation light. The photodiodes were connected to a phase sensitive detector circuit, which translates the amount of light to counts.
HIV-1 gp41 and HIV-2 gp36 recombinant antigens were diluted in buffer and applied in a 1 mm wide line on the surface of a nitrocellulose membrane. The membrane was then cut into 5 mm wide strips, so that the antigen line traversed the strip. The strips were equipped with specimen and absorbent pads (ImmunoGold�, Orgenics LTD, Yavne, Israel) These completed strips are non-limiting examples of lateral flow immunochromatography devices for use with the present invention.
Another aliquot of the above antigens was conjugated with Alexa 594 fluorophore (Molecular Probes, Eugene, Oreg., USA), employing the Alexa Fluor� 594 Protein Labeling Kit (Molecular Probes) and following the instructions of the kit. The final Moles dye/Moles protein ratio was in a range of from about 4 to about 6 and the final antigen concentration was about 2 mg protein/mL conjugate solution.
HIV-1 positive plasma specimens were obtained from the Kaplan Medical Center, Rehovoth, Israel. HIV-1 Seroconversion specimens were obtained from Serologicals Corp. (Norcross, Ga., USA). Negative specimens were obtained from Intergen/Serologicals Corp.
Each specimen was diluted 1:100 into Tris Buffered Saline with Tween-20 detergent. The fluorophore conjugated antigen mix was then added in a final dilution of 1:50. Eighty microliters of this mixture were applied on the sample pad of the above strips. The fluorescence from the antigen line of the strip was determined 30 minutes later.
The fluorescence reader was set to display 1000 counts for a blank strip and the maximal possible fluorescence reading was 13000.
Ten HIV-1 positive specimens, including seroconversion specimens, yielded fluorescence emission readings ranging from 5220 to 9800 counts. Seven HIV-1 negative specimens yielded readings ranging from 3108 to 4925 counts. These results clearly show that an exemplary device according to the present invention was able to distinguish between HIV-1 positive and HIV-1 negative specimens. Hence, the invention provides fluorescence based method and device for a sensitive detection of antibodies employing low cost and low power light emitting and light detection components.
It will be appreciated that the above descriptions are intended only to serve as examples, and that many other embodiments are possible within the spirit and the scope of the present invention.
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Corvallis 104.3 FM • Hood River 91.9 FM • Portland 90.7 FM
On this edition of "Paula's Picks", feast your ears on the melodic buffet lined up! Lots of great music and toe-tapping tunes!
As always, feedback is welcome either via e-mail: kboopaula (at) gmail (dot) com or in the "Comments" section of the "Paula's Picks" webpage right here on KBOO!
Thursday, June 17th ... it's almost officially Summer and there's no better way to ring in the season than with music.
Listen up!
The Incredible Kid loses it over Miss Pooja.
We are excited to announce our guest on this week's episode of "Indian Country", Fri. 6-11-10, will be the great flute player Charles Littleleaf !!! He is a world renown flutest and flute maker, from the Warm Springs Reservation here in Oregon.
We are excited to announce our guest on this week's episode of "Indian Country", Fri. 6-11-10, will be the great flute player Charles Littleleaf !!!
Hosted by Liz and featuring actor George Lederer reading two Sholem Aleichem stories live in our studio. Thanks, George!
Tonight's show was one of the most fun, creative and inspirational "Paula's Picks" yet! There was great music, but also AMAZING poets who were local, established and generous with their time and art.
Four poets joined us for a "Poets' Roundtable", which I hope to make a monthly series to showcase a slice of the local poetry scene. Each poet shared a few poems, some reflections and insights on poetry and their lives as poets and left us in awe!
A special four-hour edition of the Anjali and The Kid show.
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In keeping with its commitment to contribute to the excellence of the future work force, Booz Allen Hamilton has established the Vision Scholarship Program. Under this program, legal dependent children of Booz Allen Hamilton employees who are pursuing an undergraduate college degree can apply for a scholarship to help defray their tuition expenses.
The scholarship award is $3,000 per year for up to four years of undergraduate education at an accredited college or university. Five scholarships are awarded each year. The scholarship is renewable for up to 4 years, bringing the total award to up to $12,000 per student. To be eligible for renewal applicants must remain in academic good standing.
The Vision Scholarship awards will be based on demonstrated superior academic achievement, character, citizenship, loyalty, integrity, participation in extracurricular activities, and a reference from academic, professional, or business associate.
This scholarship is administered by the Community Foundation for Northern Virginia.
The program is open to legal dependent children of full-time and part-time Booz Allen Hamilton staff members residing anywhere in the world.
To be eligible for an award, dependent children must:
- be between the ages of 16 and 25
- reside with their parents or be otherwise financially dependent on them
- be enrolled in or have been accepted for enrollment in undergraduate degree programs at accredited colleges and universities in the United States.
- applications must be complete, including all attachments, by midnight Eastern Standard Time on Thursday, March 15th.
- references must be received by midnight Eastern Standard Time on Tuesday, March 20, 2012.
Dependents of officers are not eligible to participate in this program.


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Learn to swim
We love swimming and aim to pass that enthusiasm on to your child.Read more about children's lessons
We love swimming and aim to pass that enthusiasm on to your child.Read more about children's lessons
Our lessons are designed to foster confidence in the water, whatever your age.Read more about adult lessons
Our swimming lessons will put a smile on your face.Read more about our philosophy
"I would recommend Swimtime to any parent looking for excellent quality, value for money lessons for their children. Not to mention the competent, skilled staff whom my daughter adores."Charlotte, Swimtime mum
Swimming is a life skill that everyone should possess. We take this seriously at Swimtime, but that doesn't mean you can't enjoy yourself while you learn to swim with us.
Our lessons for children are fun, and we seek to help your child feel absolutely safe whether it's their first lesson, their first venture into deep water, or their first time in a more challenging class. It's our aim that your child should progress through the STA Learn to Swim Programme at their own pace, but more importantly that they develop a love for being in the water that lasts a lifetime.
If you are an adult learner, fun may not be the adjective that springs to mind when recalling your childhood lessons, but at Swimtime we think that you should enjoy your lessons just as much as our younger pupils. We want you to look forward to your time with us, and feel relaxed as you learn to swim or improve your existing swimming strokes.
Check out our video to find out more about swimming lessons with Swimtime.
Find classes near you
Please enter your postcode or town into our map search (above right). We look forward to seeing you in the pool soon.
Check out Swimtime's featured club
This week we're featuring the Village Hotel, Hull Read more >>
We teach across the UK. To find the nearest lessons to you, simply enter your postcode and click find.
Swimtime features in the Spring edition of Education for the Military Family!Find out more
During down time please don't get mad with your computer, functions will be down for just a short time.Find out more
Swimtime has been nominated for the 2015 What's On 4 Awards, as chosen by parents from across the UK.Find out more
Come and see our new pool in Garforth at our open day on Sunday 8 February.Find out more
Swimtime teachers work with school staff to get pupils swimming at Siddal MoorFind out more
Linda Price, elected to the role of British Franchise Association (bfa) Forum Chair for the North of EnglandFind out more


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do you guys also size the ring? i needed size6 and only one available is size9
The ring was a little smaller than i thought, but overall it was beautiful, very sparkly, and the best thing is the diamonds are real. Shipping was super-fast, and the price was good. Most of all she loved it....
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- Three-stone ring showcases three center diamonds highlighted with small bead-set side diamonds
- Jewelry shimmers in lustrous 14-karat white gold
- Don the look of glamour with a stunning 3-stone diamond ring
- Diamonds decorate the shoulders
- Polished, slim-style band
- Three prong-set round center diamonds
- Center diamond weight is 1/10 carat
- 62 bead-set round side diamonds
- Diamond color is I-J, clarity is I2-I3
- Total diamond weight is 1/2* carat
- Ring setting measures 21 mm wide x 3.5 mm long x 4 mm high
- Band measures 1.5 mm wide
Click here for Ring Sizing Chart
This item is part of the Miadora Wedding Collection
|Diamond Color
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|.5 to 1 Carats
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|Three Stone
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|Gold Karat
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Very Good
The ring was a little smaller than i thought, but overall it was beautiful, very sparkly, and the best thing is the diamonds are real. Shipping was super-fast, and the price was good. Most of all she loved it.Read More
promise ring
got this for my gf as a promise ring. she loves it to death and it sparkles nicely in the light. she has gotten a lot of compliments as well. it could pass for a nice engagement ring too if you are low on funds. i would recommend this ring. Thanks Overstock!!! ;-)Read More
perfect ring
I've been looking for a petite white gold 14k real diamond ring to go with a very unique anniversary ring my husband gave me for our first anniversary. And this one is perfect. The small round stones and the chips match the anniversary ring design so well. The ring may be small, but the shine and sparkle are hugh. I'm so pleased with the ring. To say it's the perfect ring for me, is an understatement. I wish you could see it together with my anniversary ring... you'd agree. Luv it , luv it, luv it.Read More
I could not have picked a better piece for my sweetheart. This diamond ring is very beautiful. The diamonds glow brilliantly, and it looks great on my darling who is so excited by it! Thank youRead More
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Hi jay1122, this ring is offered in a size 6 when available. Items are restocked as they become available, however we cannot guarantee an item will be restocked. Please feel free to check back with us periodically to see if the item has returned. Thanks for shopping with us.
Can this ring be sized??
It can but where the diamonds go all around, you want to be careful as it could distort the settings.
Shopping Guides
Top 5 Diamond Engagement Ring Styles
Getting engaged and planning a wedding will be one of the most memorable experiences in your life. Whether you are choosing an engagement ring for your girlfriend, selecting a ring for yourself or picking diamond engagement jewelry out together as a couple, you'll encounter many ring styles in the process. In addition to choosing between yellow gold, white gold and platinum bands, you will also have to select the diamond shape, ring setting and accent stones of your choice. Take a look our favorite diamond engagement rings to help you find the right one for popping the question.
Top 5 Colors in Fancy Diamond Engagement Rings
Fancy diamond engagement rings are trendy, but still unique. These rings feature colored diamonds, and they come in a spectrum of hues. Engagement rings with bright, colorful stones add a splash of color to your daily wardrobe. Fancy diamond engagement rings may feature a stunning, highly-saturated center solitaire or colorful accent diamonds and a white center stone. These diamond engagement rings really speak to a bride's individuality with an important dash of personalization.
Best Diamond Cuts for an Engagement Ring
Shopping for a diamond engagement ring is a thrilling process. The glittering stone on your ring finger that shows the world you are engaged is an important investment. You will wear your engagement ring for a lifetime, so it is important to choose a diamond ring that will represent your personality. For some, that means choosing an unconventional ring with a special cut of diamond. Consider skipping the traditional rings and selecting one of these exceptional diamond cuts for your engagement ring.
How to Choose Diamond Wedding Rings
If you're ready to pop the big question, choose diamond wedding rings for you and your beloved. A simple gold ring has been the standard in wedding bands for centuries, but many modern brides and grooms are choosing wedding rings with more sparkle. Diamond wedding rings allow you to add gems to your wedding jewelry ensemble. These rings draw attention to your prized wedding jewelry. Diamond wedding bands can be matched to your diamond engagement ring, creating a stylish combination. If you feel overwhelmed by the many choices in diamond wedding bands, allow us to help you narrow down the options and select the wedding bands that will delight you and your beloved.
What to Know when Shopping for Princess Cut Engagement Rings
A diamond engagement ring should make you feel like a princess, and a princess-cut ring is the perfect way to celebrate your romance in a regal fashion. A princess-cut diamond is square and has a lot of brilliance. Princess-cut engagement rings are neither too modern nor too old-fashioned; these rings strike the perfect balance and allow you to showcase your commitment in style. Princess-cut engagement rings are available in a range of styles, and these stones vary in quality, size and price. If princess-cut diamonds have caught your eye, use our facts to decide if this gem is right for your engagement ring.
Wedding Bands History Fact Sheet
Wedding bands and engagement rings have a rich history. Though the rings themselves have changed and cultural meanings have evolved, a ring on the fourth finger of the left hand has long been a way to show the world that you are in love. Wedding rings today can be found in traditional gold, platinum and diamond styles as well as unique gemstone, titanium and stainless steel varieties. Learning a bit about wedding band and engagement ring history can help you decide what type of wedding jewelry is right for you.
Top 5 Styles of Cubic Zirconia Engagement Rings
When you are shopping for engagement rings, you will find many featuring stunning cubic zirconias. Choosing a cubic zirconia engagement ring can give you the style you really want, even if you can't afford the same style in the diamond ring category. Cubic zirconia rings are built to last, especially if you select one with a platinum or gold setting. Whether as a temporary, affordable engagement ring or as an eco-friendly investment ring, cubic zirconia rings make stylish choices.
Vintage Engagement Rings vs Modern Engagement Rings
The wide variety of engagement rings available today means that you can have the ring of your dreams, but what if you have trouble narrowing down your options? Can you really ignore the stunning round-diamond solitaire and settle on the princess-cut three-stone ring? The first step to making a decision is to choose whether you want a modern engagement ring style or a vintage engagement ring style. Use these facts about both to determine your preference.
Top 5 Settings for Cubic Zirconia Rings
Inject more sparkle into your life with cubic zirconia rings. CZ rings are gorgeous and durable, yet they are more affordable than diamond and gemstone rings. Cubic zirconias look just like diamonds; it takes a trained gemologist to tell the difference between the two stones. Cubic zirconia rings make beautiful and budget-friendly engagement rings, as well as stunning fashion jewelry. You can buy cubic zirconia jewelry for yourself or for a loved one, but before you shop, consider these tips on CZ ring settings.
Top 5 Eternity Band Styles
Impress your wife or girlfriend on your next big anniversary with an eternity band. An eternity band is an anniversary ring that signifies your eternal relationship with an unbroken circle of diamonds or gemstones. Eternity rings can be worn next to a wedding band or they can be the perfect right-hand diamond ring. Eternity rings also work well as wedding bands; some brides opt only for an eternity band, skipping an engagement ring entirely. You have many style options when shopping for anniversary rings and diamond jewelry. Use this guide to find the perfect eternity band for yourself or for the woman you love.
What to Wear with a Cubic Zirconia Cocktail Ring
Right now one of the trendiest pieces of jewelry is the cocktail ring. Cubic zirconia cocktail rings allow women to indulge in extravagant rings without emptying their bank accounts. Cubic zirconia rings are available in a range of styles and each is very durable. High-quality cubic zirconia cocktail rings can be found in every price range. You can choose from cubic zirconia rings with sparkling clear stones or colorful gems set in sterling silver, white gold, yellow gold or gold-plated silver. Now that you are aware of the many stylish options available in cubic zirconia jewelry, use these tips to fashionably pair cocktail rings with the clothing and jewelry you already own.
Choose Your Dream Engagement Ring Setting
When shopping for engagement rings, you will find many variations of the same classic solitaire ring. In today's modern world, unique and artistic engagement rings are widely available. Avoid the carbon-copy rings and select a unique engagement ring that fits your style. Many modern brides choose colored diamond engagement jewelry or unique engagement rings made of industrial metals. Shopping by ring setting is also a great way to find an unconventional engagement ring. These unique engagement ring settings will inspire you to select funky and fashionable wedding jewelry.
How to Choose a Diamond Ring Setting
Whether you're choosing a diamond ring for yourself or looking for a ring to give as a gift, choosing the diamond setting is just as important as selecting the diamond that fits inside it. The right diamond setting will make a gorgeous diamond sparkle and shine while the wrong jewelry setting can make diamond jewelry look dull. Diamond rings, engagement rings, wedding rings and other diamond jewelry are investments, and choosing a great setting will make the jewelry twinkle for a lifetime. If you are purchasing a diamond engagement ring or a wedding ring, it is important to choose a ring setting that matches your or your bride's personal style. Showcase your diamond in the perfect ring setting with our tips.
Engagement Ring Style Glossary
When you are ready to get engaged, you will likely have many questions about the process and the traditions. Picking out an engagement ring can be a daunting task, whether you are purchasing a surprise engagement ring for your girlfriend or you are selecting a diamond engagement ring together as a couple. Possessing some basic information about engagement jewelry and wedding rings will be incredibly helpful when you are comparing diamond rings and unconventional engagement rings. Consider these common terms when you are shopping for engagement jewelry.
How to Match Your Wedding Ring to Your Engagement Ring
Commemorate one of the most important days of your life with fashionable wedding jewelry. Wedding rings and engagement rings are available in a variety of styles, making the shopping process quite involved. You have a several options for making sure that your wedding ring matches your engagement ring in a sleek and modern way. Consider the metals and stones when shopping for a wedding band and don't forget about bridal sets. Use these tips to match your wedding band to your engagement ring.
How to Buy Colored Diamond Engagement Ring Sets
Break the mold with a colored diamond engagement ring set. Colored diamonds are bold and fabulous in any shade. A colored diamond bridal set will set you apart from the crowd and give you something beautiful and bright to wear every single day. Colored diamonds are valuable, so by choosing them you are not giving up any luxury in your engagement ring and wedding band. Use these tips to shop for engagement ring sets with colored diamonds.
FAQs about Engagement Rings
If you have questions about engagement rings, then you are in the right place. Engagement ring knowledge will help you get the best diamond ring for your money and also ensure that you select wedding rings that will complement your personal style. With so many diamond engagement rings to choose from, it is important to do a little research before shopping for diamond rings. We have gladly done the research for you; check out our helpful answers to frequently asked questions about engagement jewelry.
Top 10 Styles of Unique Engagement Rings
An engagement ring is a much more personal choice than some advertisers want you to believe. It isn't just about picking the biggest diamond and the shimmeriest setting. An engagement ring represents your relationship. Your wedding rings will be something you wear every day, so it is also important that an engagement ring represents your personal style. To avoid becoming a cookie-cutter bride and a run-of-the-mill wife, choose a unique engagement ring. Unique and interesting wedding jewelry speaks for your artistic side. To find the perfect unique engagement ring for your engagement, start with these top 10 unusual engagement rings and choose the wedding rings that speak to you and your partner.
Top 5 Fashion Diamond Ring Trends
Wearing diamonds isn't just for the engaged and the wealthy. Trendy diamond rings are popping up on the fingers of fashionable women everywhere. You don't have to limit yourself when shopping for diamond jewelry. Fashion rings decorated with diamonds are available in a wide assortment of styles, and each diamond ring will add personality and flair to your wardrobe. Select rings that will set you apart from the crowd when shopping for diamond jewelry. These diamond fashion ring trends might be just the thing you need to accessorize your eclectic wardrobe.
How to Care for Your Engagement Ring
You simply adore your new engagement ring and you want it to look this lovely for the rest of your life. Proper jewelry care is important for preserving your engagement ring and wedding ring. Keeping your rings clean will keep them looking their best, but it can also help to protect the stones and settings. Storing your wedding rings properly will keep them safe and secure. Use these tips to ensure that your engagement ring will shine brightly on your 50th wedding anniversary.
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The Six Strike System: An End To Illegal Downloading?
On February 25th, the Center for Copyright Information, in cooperation with America's five largest internet service providers, launched a new "six-strike" alert system they hope will change illegal downloading for good.
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carbon monoxide safety guide:
The beautiful young woman stands on a sidewalk in Allston on a bright autumn day. Her hands are clasped in front of her. She wears an open, white cardigan and a thin, satisfied smile.
She is Binland Lee, a Boston University senior, and she is posing for this wonderful snapshot for her mother in front of the house she has rented on Linden Street.
It is fall 2012. She is proud. She is bursting with promise. Behind her, hidden by a tree whose upper branches are tinged in seasonal orange, is the attic bedroom where she has hung a beach ball-colored hammock and a poster of Hawaii's palm trees and pounding surf. It's the same bedroom where — a few months later — the 22-year-old would perish in a fire that roared through the overcrowded house she shared with 13 others.
That heartbreaking image is impossible to forget. She could have been my daughter. Or yours. Or your sister, or your roommate, or the funny and trusted friend you've always treasured.
My Spotlight Team colleagues and I spent months investigating overcrowded and dangerous conditions that plague Boston's student neighborhoods. What we found — as detailed in a series of stories published in May — was a poisonous stew of greed, mismanagement, and neglect, unchecked by city officials, that threatens the health and safety of students in America's college capital.
We found doors that would not lock, windows that would not close, units without heat, bedrooms shoehorned into rat-infested basements, and fire-trap attics. And we found that the city was doing next to nothing to enforce its zoning law that prohibits more than four full-time undergraduates from living together.
Greedy landlords, who know the city's well-documented inspectional impotency, cram in as many kids as they can to pay for a real estate investment that has been their golden goose. The kids, looking for the cheapest rent possible in a pricey market, are often willing accomplices.
I bring this up because as of today, all of that is supposed to change. Today is the deadline for the city's colleges and universities to report the addresses of their off-campus undergraduate students to Boston's city clerk. Officials will build a database that will allow inspectors to detect overcrowded units. So imagine my excitement on Friday morning when I got a chance to chat with the city's new chief inspector. He is William "Buddy" Christopher, the commissioner of Boston's Inspectional Services Department.
Armed with this new data, certainly the city is about to crack down, flood the neighborhoods with inspectors, and punish landlords who continue to break the four-housemate rule. Instead this is what Commissioner Christopher said: "That is not a top priority for me.''
What? Days after the Globe series, Mayor Martin J. Walsh called us in our Spotlight office and promised swift action. "My concern is the life of every young college student living off campus in overcrowded apartments,'' Walsh said.
But now, Commissioner Christopher, is essentially saying "never mind.''
Moments after I hung up from Christopher, his spokeswoman called me to say he had misunderstood my question. Perhaps he thought I had asked him about his color preference for the drapes in his office.
No. This was no misunderstanding. Christopher told me the city has hired no new inspectors to specifically target overcrowded student rentals. And do you know how many landlords since May the city can name that it has cited for violating the zoning amendment designed to protect the 45,000 college students who are a vital part of the city: zero.
Seriously, Barney Fife had more authority in Mayberry.
The Globe even handed Christopher and his predecessor clear examples of overcrowded conditions on a silver platter. We published the name of the landlords, the offending addresses, the number of tenants, along with photographs of the units. I followed inspectors days later to those addresses. They knocked on doors and, when they got no answer, they left notices on the doorstep.
The follow-up since then? Again, zero. You've really got those greedy landlords shaking in their boots, Buddy.
Christopher did pay lip service to a broader approach to dangerous housing conditions in student neighborhoods, targeting problem properties as the best route to address the issue. Fine. But his dismissal of the urgency to enforce the no-more-than-four zoning rule is outrageous. And it's irresponsible.
By the way, the problem remains widespread. How do I know? I did something this week that apparently is beyond the skill set of city inspectors. I walked the student neighborhoods, knocked on doors, and talked to students, many of whom acknowledged that they — and most of their neighbors — were in violation of the rule.
Enough is enough. As of today, the city is out of lame explanations and phony excuses.
If I were Marty Walsh, I'd call an emergency staff meeting for Monday morning for his inspectional services staff and code enforcement team.
It can be the shortest staff meeting in the history of that blocky bunker of a place on City Hall Plaza. Here is all the mayor has to say to his assembled ISD staff: Get serious. Get to work.
Read more:
Here's a Halloween safety guide for parents: http://www.protectamerica.com/static/pdf/halloween-family-safety-security.pdf
Was your grant request turned down? Read this to find out why…
Grant rejection letters are never easy to take; here's a deeper look at the three types of letters and why they are sent. Can I appeal?
A woman suffered smoke inhalation when flames broke out in her home early Monday. And, Philadelphia firefighters say that hoarding hindered their efforts as they fought back flames in the woman's Olney section home.
The flames broke out shortly around 2 a.m. in a corner row house at W Ashdale and 3rd Streets.
A 60-year-old resident suffered some burns and suffered smoke inhalation includoing soot in her mouth. Crews rushed her to Temple University Hospital with non-life-threatening injuries.
Firefighters remained at the home putting out hotspots for hours. They said that even though they made quick work putting out the flames that their efforts were hindered by a potential hoarding situation in the home.
#hoarding#clutterhttp://www.iaff.org/14News/051914Hoarding.htm …pic.twitter.com/MzMGlOiWnT"
Read More…
When you think about your "work environment" as a firefighter, do your thoughts jump immediately to the station? In fact, your work environment is much, much larger.
"One of the things I like to ask firefighters is, 'How can I improve your work environment?'" says Sean DeCrane, battalion chief for the Cleveland (Ohio) Fire Department. "Some people say they need exhaust systems on the apparatus floor, others answer that they want to separate the turnout gear from the living quarters to protect firefighters from exposure to carcinogens—and then of course you get the guy who wants a big flat screen TV for the training room. Those are all good improvements, but they're not improving the working environment; they're improving your staging area."
In military terms, DeCrane says, the staging area is where you gather supplies and troops, where you prepare for deployment and where you regroup and rest. "Where you deploy is your area of operation—and in the fire service, those are the buildings we respond to and operate in. And many times when we do that, the buildings are under duress," he says.
This is the basis for DeCrane's quest to get firefighters more involved in the building code—as well as the message behind his FDIC seminar held today, "Firefighter Safety and the Codes: a Necessary Partnership."
read more:http://www.firefighternation.com/article/firefighter-safety/why-firefighters-should-care-about-building-code


text:
Expanding the memory of a computer
User has a computer which has four slots that can have 128 MB, 256 MB, 512 MB, 1 GB and 2 GB. Two slots contain 512 MB memory modules. In order to expand the memory to 4 GB user need to add a 1 GB module and a 2 GB module in the remaining two slots.
As there are 4 slots and all of them can have maximum of 2GB memory. So, maximum memory supported by such a computer is 8 GB if 2 GB memory module is in every slot.
Chegg is one of the leading providers of homework help for college and high school students. Get homework help and answers to your toughest questions in math, calculus, algebra, physics, chemistry, science, accounting, English and more. Master your homework assignments with our step-by-step solutions to more than 3000 textbooks. If we don't support your textbook, don't worry! You can ask a homework question and get an answer in as little as two hours. With Chegg, homework help is just a few clicks away.


text:
Black and White Bathroom
floor, carrera marble vanity top, sink, faucet
a black perimeter is cute
same floor and vanity top as before
do I want the floor to make this much of a statement?
black vanity
could think about going modern instead of period
never would have thought to use tiles this large
is that vanity espresso or black?
black ceiling?
never would have thought I'd go in this direction
half glass shower wall in front of windo
all white
inset basket weave in larger squares
love this even though not black and white
like the colors, not the stuff
love the wall color, the diamond carerra floor
interesting floor inset
black vanity, neutral, textured walls


text:
Here are Sprouter's hot startups for the week of November 9.
Maptia is on a mission to build the most inspirational map in the world – the most delightful way to discover, collect, and share the places you love and to explore the world around you. It will be a beautiful discovery engine for places.
Taurus.io lets you quickly and easily set up a product tour for your web app in just 15 minutes. Load the Taurus app into any web application, and start showing users around your product, and directing them to new features.
Tred lets you test drive and purchase cars directly from your driveway. Customize the car of your dreams on their website, see reviews from other users, and their team of concierges bring the car to your driveway so you can take it for a spin.
Summly provides beautiful and concise summaries of news articles directly to your smartphone. Select your favourite news source, and topics, and Summily algorithmically generates summaries giving you all the info you need.
Get news on hot startups such as these plus much more every week in the Sprouter newsletter. Sign up now!
Looking for great local deals? Find hundreds of items on sale at discounts of up to 50% at LikeitbuyitEdmonton.com. Browse now, as new items are being added daily until April 8. Don't delay; savvy shoppers are already planning their purchases. If you like it, buy it before it's gone! Presented by the Edmonton Journal.


text:
Law & Order
The Dead Wives Club
- Episode Description
When a woman abandoned by her firefighter husband for his partner's widow is threatened with also losing custody of her children, she murders her romantic rival, claiming that 9/11 post-traumatic stress syndrome prompted her actions.
- Cast & Crew
Elisabeth Rohm
S. Epatha Merkerson
Sam Waterston
Jesse L. Martin
Dennis Farina
- Fan Reviews (1)
There's a name for Jack McCoy, and it's not pretty.
- Trivia & Quotes
Quotes (9)
Jack McCoy: (Cross examining the defendant) In fact, as far as I can tell, the first time you mentioned this problem to anyone was you raised it as a defense for killing Donna McClean. Vanessa Galliano: Objection! Jack McCoy: That's alright. She doesn't have to respond. We all know the answer.
(Defense attorney examines the defendant's ex-husband) Vanessa Galliano: Let me get this straight, Mr. McClean. You left your wife, the mother of your children, by your own testimony depressed and emotionally traumatized by the events of 9/11, so you could marry her newly rich best friend, your best friend's widow. Ed McClean: It wasn't like that. Vanessa Galliano: Wasn't it?
Serena Southerlyn: (Talking about the Collette Connolly case) You don't think that there is some room for leniency here, Jack? Jack McCoy: Absolutely, from the judge, at sentencing.
Joe Fontana: When you were a kid, did you ever call your dad by his first name? Ed Green: Not unless I wanted to smell the Aqua Velva on the back of his hand.
Jack McCoy: It's a run-of-the-mill murder with a headline defense.
Notes (1)
Before the opening credits, a title card is shown with the chiming noise. This rarely occurs on the series.
Allusions (4)
This episode appears to be ripped from the headlines of the 2003 Staten Island Ferry crash.
Firefighter: They were a regular Frick and Frack.
Frick and Frack were the stage names of Swiss comedic skaters Werner Groebli and Hansruedi Mauch, who came to the US and became famous performing in the Ice Follies. Their names became synonymous with people who shared a close work partnership, becoming a common slang term for such a pair. Mauch died in 1979; Groebli continued performing as Mr. Frick for many years before retiring from skating.
Ed Green: She doesn't look like Marion Jones to me.
Marion Jones is a former American track star and Olympic gold medalist. Unfortunately, she has recently admitted to using steroids and been forced to return her Olympic medals. Green was saying that Collette Conolly would have had to run extremely fast for her alibi to hold up.
The title of this episode, The Dead Wives Club, is a play on The First Wives Club, a novel by Olivia Goldsmith about a group of 'first wives' who get even with their ex-husbands. It was later made into a movie starring Bette Midler, Diane Keaton and Goldie Hawn as the vengeful ex-wives.
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text:
Oct 06, 2012 at 10:20AM EDT
Hello PogoBox! :D
Why, thank-you, but that's sad to hear there isn't many Regular Show fans around here! There needs to be more :) Glad to hear you're from Australia too and you're a RS fan and you're on KYM, which is awesome :D Thanks so much!! I will and thanks again C:
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text:
Dr. David A. Rawitscher, MD
Cardiovascular Disease
- 5425 W Spring Creek Pkwy Ste 260
Plano, TX 75024
- 2 Locations
- (469) 800-6300
Dr. David A. Rawitscher graduated from the University of Texas Medical School at Houston in 1991. He works in Plano, TX and 1 other location and specializes in Cardiovascular Disease. Dr. Rawitscher is affiliated with Baylor Medical Center At Frisco, Baylor Medical Center At McKinney and Baylor Regional Medical Center At Plano. He speaks English and Spanish.
Gender: Male
Languages Spoken: English, Spanish
Cardiovascular Disease
Medical School: University of Texas Medical School at Houston; Graduated 1991
*This physician has multiple office locations. See Office Information for details.


text:
Upcoming events April 25 - May 2
Wednesday, April 25
Take a trip half way around the world for free in the comfort of the local library with professional photographer Cal Rice.
The presentation begins at 1 p.m. today (Wednesday) at the Park Rapids Area Library.
Rice will lead a "trip" to Bhutan: A View from the Top of the World, showing the beautiful landscapes and the people amidst massive changes.
Are you a newspaper subscriber but you don't have a Digital Access account yet? https://secure.forumcomm.com/?publisher_ID=40&event=subscriber/lookup.
You will need your subscription account number and phone number. Not sure if you have an account? Email us at email@example.com and we can help you.


text:
Boulogne-Billancourt, FRANCE -- Vallourec announced the full qualification of its new finishing plant in Saudi Arabia. Located in Dammam, this unit is dedicated to the heat treatment and threading for the full range of VAM premium connections, with an annual capacity of 100,000 tons. It will mainly supply Saudi Aramco, the Kingdom's national oil company, along with other regional operators. With the qualification of this plant, Vallourec is reinforcing its presence in the Middle East where numerous oil and gas projects require high added-value tubular solutions.
Resulting from the acquisition in 2011 of Saudi Seamless Pipes Factory Company Limited - "Zamil Pipes", the finishing plant comprises a heat treatment unit, a new threading unit and coupling shop (the last 2 built by Vallourec). It is now qualified to carry out all operations required in the production of premium connections using hollows provided by Vallourec's pipe mills.
The new plant is expected to generate around 100 jobs, bringing the total number of employees working there by the end of 2013 to 200.
Didier Hornet, Managing Director of Vallourec's Oil & Gas Activities, said:"The qualification of our new upgraded finishing plant by Saudi Aramco gives Vallourec a clear competitive edge in the Kingdom and in the Middle East, where the demand for local content is increasing. This investment will enable us to support local customers with the most advanced premium products for their growing gas projects, and with increased services thanks to higher flexibility and shorter delivery times."
The new finishing plant has already received an order for 20,000 tons of premium connections.
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text:
- Exchange: -
- Sector: Energy
- Industry: Renewable Energy
- Sub-Industry: Renewable Energy Equipment
Sooner Holdings Inc
SYNM:USTicker Delisted
Company Profile for Sooner Holdings Inc (SYNM)
Sooner Holdings Inc is a producer of synthetic polyurethane leather for the shoe industry in the People's Republic of China.
Contact Information
Sooner Holdings Inc
5416 South Yale Avenue
Suite 400
Tulsa, OK 74135
United States
Phone: 1-918-592-7900
Fax: 1-918-592-7979
Key Executives for Sooner Holdings Inc (SYNM)
Quotes delayed, except where indicated otherwise. All prices in local currency. Time is ET.
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text:
Examining the raging debate: risks v. benefits and parents' right to make choices about their child v. the needs of a community.
FRONTLINE reports from Iraq on the miscalculations and mistakes behind the brutal rise of ISIS.
With Yemen in chaos, a gripping report from the heart of the escalating conflict.
No one who has seen this documentary can ever forget it . One critic said it has the power "to shake the soul."
Get Our Newsletter
Follow Us
A conversation with
his biographer, assessments of his character and politics, and a look at his relationship with George W. Bush.
Robert Kagan and Will
Hutton debate the myths and realities of the U.S.-Europe divide. Plus, comment and analysis on the costs of U.S. unilateralism.
Paul Berman, Timothy
Garton Ash, and David Rieff discuss Blair, the liberal case for war, and
the divided left.
An overview of the key diplomatic moments
and missteps leading to war with Iraq.
introduction : the prime minister : the fractured alliance : the liberal divide
the failure of diplomacy : interviews : readings & links : discussion : producer's chat
tapes & transcripts : press reaction : credits : privacy policy
FRONTLINE : wgbh : pbsi
For the past few months, British Prime Minister Tony Blair has been fighting the biggest political battle of his career. Caught in the center of a high stakes political storm, he tried to personally bridge the gap between the United States and its European allies -- particularly France and Germany -- over the impending war in Iraq. FRONTLINE examines the roots of the discord within the Western alliance, the perilous role Blair has played, and the stakes for him and the West should this old alliance fall apart.
published april 3, 2003
photograph copyright © najlah feanny/corbis
web site copyright 1995-2014
WGBH educational foundation
FRONTLINE on


text:
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Valid through March 28, 2014. The restaurant is closed on Mondays. Valid Saturday-Thursday only or Fridays only, depending on the voucher purchased. PLEASE TIP ON THE FULL VALUE OF THE CHECK; 18-20% is customary for good service. Reservations are required; subject to availability. Valid for two diners only. Limit two vouchers per table. Not valid on major holidays or holiday weekends, including Valentine's Day weekend. This deal cannot be combined with any other offer or promotion.See all terms
M – F, 9am – 9pm ET
|Enjoyed Most
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The food was delicious!!
Non-traditional appetizers and sushi rolls, very inventive. Not you traditional California rolls, etc.
|May 16, 2014 Lauraine California, United States
The food was exceptional
|May 14, 2014 Debra California, United States
Food and service were both excellent.
|Apr 07, 2014 Monika CA, United States
The service was outstanding and the food was delicious.
I will go back for sure and recommend them for catering.
|Mar 30, 2014 Kathleen
Restaurant was clean with a nice ambience. Sushi was very fresh and yummy. I liked the choice if omikase they served. Overall it was a great experience.
The service was just average. The waitresses were not very attentive. I had to wait a 4-5 min before I was given a menu. And my table had an empty soy sauce container, and no server came around for another 4- 5 minutes, so I just got up and took it from another table. It was frustrating.
|Mar 29, 2014 Tess California, United States
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text:
Pacific Seafarers Net
During the last 30 days this link has been reported as broken.
Check related links to this in DX Resources/Nets/Emergency Nets
The Pacific Seafarer's Net operates from 02:30Z to 04:00Z, 7 days a week, 365 days a year on 14.313MHz and sometimes on 14.300MHz. Between 02:30Z and 03:24Z we perform general traffic handling, and then at 03:25Z the rollcall portion of the Pacific Seafarer's net begins.
[ Hits: 342 | Votes: 0 | Rating: 0.00 ]
About Pacific Seafarers NetThis resource is listed in dxzone.com under 1 categories. Its main category is Radio nets for disaster help (Amateur radio nets to help in disaster events). "Pacific Seafarers Net" is listed in our web site index since Sunday Feb 6 2005 and till now received a total of 342 hits.
- DX Resources/Nets/Emergency Nets - Amateur radio nets to help in disaster events
Rate this resourcePacific Seafarers Net is not rated yet.
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Webmaster, add a Remote rating to your site.
Pacific Seafarers Net - similar linksWe thought you might be interested also in these additional resources, currently listed under our DX Resources/Nets/Emergency Nets category:


text:
"Dose not make the masrk."
Bourdain , dose not make the mark as he did in Kitchen Confidential. He is on a soap box to berate his peers in very lengthy and winy explanations. He lost credibility on this one .
"Ken Follett at his best."
Ken Follett at his best. I loved this audio book. Mr Lee as usual was at his best. A wonderful audio book.
"good ,but not his best"
Good ,but not his best. His writing is entertaining as usual. The reader dose a fine job. The story is not his best. But I did enjoy the book and it was hard to put down.
"Kept my interest"
The reader was did a very good job. I found the content excellent and it kept my interest..I recommend this audio book.
"Fun Read"
This story kept my interest all the way, also the narration was very good. A fun read.
"Great read , Naration by O'Reily is excellent"
Naration by Bill O'Reily is the best. I know there is inacuracies in some of the details. But extremly entertaning.
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If you find this review inappropriate and think it should be removed from our site, let us know. This report will be reviewed by Audible and we will take appropriate action.


text:
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- date unknown
Inflation is down.
- date unknown
linked to #228351
- Guybrush88
- Jun 26th 2014, 17:25
linked to #3343415
- sharptoothed
- Dec 16th 2014, 13:58
linked to #3695692
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text:
BBB Business Review
BBB Accredited Business since
BBB Business Reviews may not be reproduced for sales or promotional purposes.
This business states they can help you create the video you want, start to finish - concept, writing, videography, including expert lighting and sound, editing, formatting, DVD copies, website video.
Request a Quote
BBB Accreditation
A BBB Accredited Business since
BBB has determined that I-Conn Video Production meets BBB accreditation standards, which include a commitment to make a good faith effort to resolve any consumer complaints. BBB Accredited Businesses pay a fee for accreditation review/monitoring and for support of BBB services to the public.
BBB accreditation does not mean that the business' products or services have been evaluated or endorsed by BBB, or that BBB has made a determination as to the business' product quality or competency in performing services.
Reason for Rating
BBB rating is based on 13 factors. Get the details about the factors considered.
Factors that raised the rating for I-Conn Video Production include:
- Length of time business has been operating
- No complaints filed with BBB
Industry Ratings Comparison | Chart
Customer Complaints Summary
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Additional Informationtop
Type of Entity
Business ManagementMs. Nancy C Adams, President Mr. Matthew Starr, VP
Contact Information
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Video Production Services Advertising Agencies & Counselors Public Relations Counselors Training Programs
Products & Services
I-Conn Video Production offers the following product(s): "An Abuse-Free Life", the Roger MacNamara(TM) Series - 5 DVDs and User's Guide


text:
218 viewings on 10 March, 2015.
Taken with my Lumix G3
Top 10 in challenge in "Shelters" group
4th place out of 22 entries
Shelters Along A River or Canal or in a calm Harbor Challenge
How beautiful!
Hi Keala, Ljubljana is a lovely place. In fact Slovenia in general is and the country people are very friendly even the one who don't speak English but are prepared to help lost Australians who have "gone bush" to check out the rural areas..
– Margaret Hyde


text:
Stock Photo:
Group of industrial workers with yellow helmet isolated over white background.
- Image ID: 92792374
- Release Information: Signed model release filed with Shutterstock, Inc
- Copyright: kurhan
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text:
Kingswood vs St. Anthony's match suspended due to brawl involving the teamsWritten by Administrator
- Details
Category: rugby
Published: Thursday, 27 June 2013 18:24
Hits: 3535
Kingswood vs St. Anthony's match suspended due to brawl involving the teams.. KCK 6 v SACK 5
3rd Class Work From Kingswood College.Need To Be Shame For That Behaviour.
kingswood players got injured after the so called HAKA.... what a funny incident.. they didn't know there were hundreds of video cameras rolling. no brain like stray dogs......!
Well Kingswood will come good since the tainted principal of Kingswood who is the president of the Schools body who also cleared Vidyartha last year and gave the verdict which bemused the Great Kaplan who was here. He will make sure Kingswood go scot free. If he has dignity or self integrity he will make that bold move by banning the team for causing the rift.
I am not surprised at all. Kingswood is the Isipatana of Kandy. We have gone to Kandy many times to play them over the years and has had the bus stoned or players hit by poles. So I am not surprised at all.
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