text:
Warren, MIUSA 48093
July 13, 2013
Screenwriting, and I'm able to do a little of everything for the most part.
Screenwriting and acting.
Lapeer, Michigan
I'm 18 years old, I attended Interlochen Arts Academy for a year, where I studied in the Motion Picture Arts program. My strengths are screenwriting, acting, and some aspects of production.
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Highlight: Ed Ruscha
Spotlight: Wright
Featured: Stephen Bulger Gallery
Now On: Herbert List
Top Lot: Anish Kapoor
View Catalogue: Donald Lipski
Over 9 MillionAuction Results
Featured Member:Cavalier Galleries
Upcoming Auction atPhillips New York
Sample Report
Cup Form Frost
Price on Request
WILSON A. BENTLEY (1865-1931): SNOWFLAKES, FROST, DEW, CLOUDS will consist of about fifteen vintage photomicrographs of snowflakes, frost, and dew, as well as photographs of cloud studies, taken by Wilson A. Bentley, better known as "Snowflake" Bentley.
WILSON A. BENTLEY coined the phrase "No two snowflakes are alike." He grew up in Jericho, Vermont, and developed a life-long fascination for snow. His parents bought him a camera with a microscope attached with which he discovered how to photograph snowflakes. The process was difficult, made more so by the temporary nature of the subject – many snowflakes melted before BENTLEY could capture their images on film, making the photographs extremely rare.
BENTLEY was the subject of a Caldecott Medal-winning book, Snowflake Bentley, by Jacqueline Briggs Martin (Houghton Mifflin Company, 1998), and a biography by Duncan C. Blanchard, The Snowflake Man: A Biography of Wilson A. Bentley (McDonald & Woodward Publishing Company, 1998). BENTLEY himself published numerous articles about his work in magazines such as National Geographic, Harper's Monthly, Popular Science, Popular Mechanics, and The New York Times Magazine, as well as a book, Snow Crystals, later republished as Snowflakes in Photographs (Dover Publications, 2000).

subset: commoncrawl


text:
Help with a Baby Red Banana plant
diana55(z8a)March 22, 2007
I was given a little Banana plant and I forgot about it. I left it in the back of my car for about a week. The leafs are all dead on it but the corm is still moist. It is about 6 inches tall. I planted it in a pot and put it in a bright window. Can it still be saved? Is there anything I can do for it? Any help would be appreciated!!! Thank you!!! Diana55


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The core precision tank is a perfect fit, very slimming! I really feel the sup- port in my back, I've never tried on a tank like it. Supportive undergarments are very important when working out and I think they've mastered it.JARRET JANAKO ACTOR AND MODEL
EQUMEN™ Helix-Mapping™ Technology seamlessly builds in physiotherapy insights to deliver ergonomic results. See more details
Over time, the aspects of modern life can be as hard on the body as the challenges in sport. From climbing the corporate ladder, to sitting at a desk all day, to chasing success - all while just finding time for the gym, every man needs and deserves the advanced benefits available to elite athletes to improve their daily performance.
Human performance and daily well-being begin with the core. Every man can benefit from enhanced:
Core Alignment: When the skeleton is correctly aligned, the bones support the weight of the body with minimal stress. This ensures that muscles hold very little excessive tension and they can then efficiently initiate and sustain movement. Proper core alignment also facilitates oxygen flow for enhanced energy.
Core Stability: Core stability allows you to maintain a solid foundation and transfers energy from the center of the body out to the limbs. Ergonomic support in the trunk area is essential for the maintenance of upright posture, back pain minimization and injury prevention.
Temperature-Control: Thermoregulation is part of a homeostatic mechanism that keeps the body at optimum operating temperature. In humans this optimum temperature is 36.8 °C (98.2 °F). Assistance in maintaining this temperature can help to optimise energy efficiency.


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Thursday, April 11th, 2013 at 1:00pm
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The Ethel String Quartet performs Robert Sirota's "ETHEL Fanfare" for string quartet.
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Lion HillsAvailable March 30th at 5:30pm
News Twitter Feed
Extension Service Teams Assess ag Disaster Sites
MSU Students Earn "Scholars" DesignationMay 2, 2014 | Steve RogersNineteen Mississippi State seniors are new members of the university's prestigious Society of Scholars in the Arts and Sciences.
MDOT Encourages Everyone to "Share The Road"
GRAPHIC: MSEMA, FEMA Release Information Graphic for Debris Removal Procedures
Louisville Schools Seek Sense of NormalcyMay 1, 2014 | Steve RogersAttendance at 88 percent in first day back.
Video: EMCC Hosts Annual Job FairMay 1, 2014 | Justin MinyardJob seekers and companies looking for good workers are in one place today.
Video: MSU Student Gets Quite a SurpriseApril 30, 2014 | Eric LampkinThe Alumni Delegates "Prize Patrol" armed with balloons and a big check, surprised Harris Ward as he came out of class.
Staff, Professors, Students Receive MSU Research HonorsApril 30, 2014 | Steve RogersFifty Mississippi State students, faculty and staff are 2014 selections for exceptional research and leadership honors.
UMMC Prof Named Tops in SECApril 30, 2014 | Steve RogersDr. John Hall, a professor and administrator at the University of Mississippi Medical Center, has been named the Southeastern Conference's 2014 Professor of the Year in recognition of his excellence both in the classroom and as a topflight obesity and cardiovascular researcher.
MSU Renames Coliseum Drive as 'Bailey Howell Drive'April 30, 2014 | Robby DonohoSTARKVILLE, Miss. (WCBI/MSState.edu) – Mississippi State University's east-west street near the Humphrey Coliseum basketball venue has been officially renamed in honor of MSU and NBA basketball great Bailey Howell. The […]
Wednesday Closings 4/30/2014April 30, 2014 | Mary Carroll SullivanColeman Head Start remains closed today because of a power outage.
UPDATE: Wednesday Schedule for Lee County SchoolsApril 29, 2014 | Michelle LoweAll Lee County Schools will be open tomorrow, Wednesday, April 30, 2014, except Guntown Middle School and the Saltillo area schools due to power outages.
UPDATED: Weather Related School and Business Closings for Tuesday April 29April 29, 2014 | Mary Carroll SullivanEARLY DISMISSAL ICC All-American Band concert cancelled for tonight Choctaw Co Schools at 12:15 P.M. Houston Schools at 12:15 P.M. Fayette County, AL Schools at 12:00 p.m. Pickens County, AL […]
Ole Miss Arbor Day CelebrationApril 28, 2014 | Paulo SalazarOle Miss wrapped up green week by adding two new dogwood trees to the Grove.
Tupelo Addressing School FundingApril 28, 2014 | Jamie ScrivenerTupelo talks fully funding for public schools.
Video: Vernon Residents Discuss Historical Figure
Video: Ole Miss Considers Carbon Monoxide Monitors
Video: Immanuel Christian School Needs New Name
Video: Tupelo High Marks Big MilestoneApril 26, 2014 | Emily CassuloThe largest public school in the state of Mississippi is celebrating its 100th birthday.
Residents Dance Like Elvis for New EventApril 26, 2014 | Emily CassuloSome North Mississippians are putting on their blue suede shoes to learn how to dance like the King of Rock n' Roll himself.
Video: Tupelo's Boerner to Run Challenge Makes Its Second RunApril 25, 2014 | Eric LampkinTupelo's Boerner to Run Challenge Makes Its Second Run
Video: The Starkville Boys and Girls Club Honor Kids For Completing Life Coach ProgramApril 25, 2014 | Heather BlackThe Starkville Boys and Girls Club Honor Kids For Completing Life Coach Program
Video: FLIGHT TEST: Airmen Begin First Steps Toward Their WingsApril 25, 2014 | Emily CassuloHundreds of student pilots call North Mississippi home for at least a year as they train at the Columbus Air Force Base to become the Air Force's newest aviators.


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Serves meat, veg options available. A traditional pub/hotel. Offers several vegetarian dishes and will make vegan (using tofu, etc) with advanced notice. Price: Inexpensive more-less
Vegan-friendly, Lacto, Ovo, Beer/Wine, Non-veg
1 George St (York Penisula)
Moonta, South Australia 5558
Moonta, South Australia 5558
- Wheelchair accessible
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Diane Sawyer
Diane Sawyer anchors for last time
Sawyer has anchored ABC's flagship newscast since December 2009.
Watergate scandal: 10 legacies
Four decades after Nixon's baleful farewell, here are 10 lasting legacies of the Watergate era.
ABC News apologizes for mistaking Palestinians for Israelis
The network apologizes for mistaking a photo of Palestinians for Israelis.
Diane Sawyer leaving ABC 'World News'
Diane Sawyer is stepping down as anchor of ABC's "World News" but will stay with ABC for specials and events, the network announced on Wednesday.
Clinton's hardest words: I was wrong
Her out-of-the-gate stumbles in interviews this week reflect an issue she's faced repeatedly.
The media vs. Hillary Clinton
The press is ready for Clinton to run.
Clinton clarifies 'dead broke'
She revisits earlier comments to Diane Sawyer.
Clinton: Benghazi more reason to run
She says she "was not making security decisions."
5 tests for Clinton's book tour
She begins a frenetic round of interviews.
Christie: Scandal won't affect 2016
He says it won't change the calculus on his future plans, including a possible presidential bid.
Sawyer to interview Ann Romney 'hours before convention speech'
The ABC anchor will interview the GOP hopeful's wife before her convention speech.
Ann Romney: Seamus 'loved' car roof
Says it was better than leaving "him in the kennel."
An appreciation: The day I was scooped by Mike Wallace
On coming in second to the "60 Minutes" legend.
ABC: Giffords, Kelly open to a child
They reportedly continue to discuss the possibility.
Giffords gives first TV interview
In her exchanges, the lawmaker, who was shot in January, seems confident and smiles.
Tweets of the day: Sawyer, Perry, Tapper
Blog: Monday's notable tweets.
Giffords interview not a done deal
The Democrat "has not decided" whether she'll go on-air with Diane Sawyer, a spokesman says.
Amid fanfare, Larry King signs off
His farewell show was ultimately less about his guest roster and more about the host himself.
Dems await Pelosi's big decision
Pelosi has been expected to step aside.
Whitman coy on ending spending
She dodges questions on whether she intends to dig deeper into her own pockets.


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Bike, truck crash injures local man
WILLMAR — A pickup truck crashed into a bicycle Tuesday morning in Willmar, sending a 53-year-old man to the hospital for injuries.
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Writers Profile
Screen Name: Kaleepeare
Kaleepeare has published 29 items on The Spoof.
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Bush CopyrightGeorge Bush Senior has taken out copyright on the phrase "New World Order" that he made popular. Anybody using the phrase without his permission will be sued.
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Click Here for St. Valentine Prayer's Valentine was a holy priest in Rome, who, with St. Marius and his family, assisted the martyrs in the persecution under Claudius II. He was apprehended, and ... continue reading
Walfrid or Galfrido della Gherardesca was born in Pisa, of which he became a prosperous and honored citizen. He married a wife to whom he was deeply attached, and they had five sons and at least one ... continue reading
Born in Corinaldo, Ancona, Italy, on October 16 1890; her farmworker father moved his family to Ferrier di Conca, near Anzio. Her father died of malaria and her mother had to struggle to ... continue reading | shop
The name Gabriel means "man of God," or "God has shown himself mighty." It appears first in the prophesies of Daniel in the Old Testament. The angel announced to Daniel the prophecy of the ... continue reading | shop
Saint Kateri Tekakwitha, Virgin, was canonized on 10/21/2012 by Pope Benedict XVI. Kateri was born near the town of Auriesville, New York, in the year 1656, the daughter of a Mohawk ... continue reading | shop
Considered by some historians to be the founder of the Carmelite Order. He was born in Limoges, France, and proved a brilliant student at the University of Paris. Ordained a priest, Berthold joined ... continue readingMore Saint of the Day
Our Lady of Guadalupe December 12 (USA) When we reflect on the feast of Our Lady of Guadalupe we learn two important lessons, one of faith and one of understanding. Missionaries who first came to Mexico with the conquistadors had little success in the beginning. After ... continue readingMore Female Saints
St. Michael the Archangel - Feast day - September 29th The name Michael signifies "Who is like to God?" and was the warcry of the good angels in the battle fought in heaven against satan and his followers. Holy Scripture describes St. Michael as "one of the chief ... continue reading
The name Gabriel means "man of God," or "God has shown himself mighty." It appears first in the prophesies of Daniel in the Old Testament. The angel announced to Daniel the prophecy of the seventy weeks. His name also occurs in the apocryphal book of Henoch. He was the ... continue reading
According to his legend, Sebastian was born at Narbonne, Gaul. He became a soldier in the Roman army at Rome in about 283, and encouraged Marcellian and Marcus, under sentence of death, to remain ... continue reading
There is a romantic legend that the mother of Thomas Becket was a Saracen princess who followed his father, a pilgrim or crusader, back from the Holy Land, and wandered about Europe repeating the ... continue reading
By Alex Basile
Every carpenter must practice patience. We can learn important lessons from the wood shop in Nazareth from the humble Saint Joseph. I have always been a "do it yourself" type of guy thanks to my father. My dad is always a steady presence during my home-improvement ... continue readingMore Christian Saints & Heroes


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Pelham Bay Park
Terrarium Workshop
Saturday, November 16, 2013
12:00 p.m.–2:00 p.m.
Please note: This event has already taken place. Please use the Search options on the right to find upcoming events.
Winter is coming!
Tropical plant terrariums, hanging glass globes with air plants, succulent and moss dish gardens, and more will get you through the long winter months.
Join horticulturalist Maria Colletti to learn how to make a miniature garden—and take it home.
Registration required.
$10; seniors, students, & members $8. $20 materials fee.
Event Organizer
Contact Number
(718) 885-1461
Contact Email
Directions to Pelham Bay Park
Pelham Bay Park Weather
- Mon
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- Historic House: Bartow-Pell Mansion Museum
- NYC Parks Kicks Off Summer Beach Season In New York City
- Go Explore The Wonders Of Winter
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- Kids Week Bronx: Orienteering for Kids
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- Yoga in the Orangerie: Intermediate Level
- Barbecuing Areas
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- Nature Centers
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- Roller Hockey
- Running Tracks
- Soccer Fields
- Spray Showers
- Tennis Courts
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Pelham Bay Park


text:
A maximum sensitivity optical scanning system is disclosed. It finds use in a variety of applications, including the reading of biopolymeric arrays. It operates by scanning sample at a setting selected to result in signal saturation for some, but not all available data. Subsequent scans of the same area are taken at lower sensitivity settings (in terms of detector gain and/or excitation light source gain or attenuation) and data from at least the previously saturated regions is obtained. If system sensitivity is set too low to produce useful results, optional features may adjust sensitivity upward and follow with an increased sensitivity scan as a remedial measure. Full signal sensitivity is better preserved as most needed in taking data for the weakest signals first with the high-level scan. Data for sample producing stronger signals that can better tolerate photobleaching is then taken in one way or another.
1. A method of optically scanning a sample in connection with a biopolymer array, said method comprising:
providing a biopolymer array;
performing a first automated scan of said array to detect said sample, wherein at least some results of said scan are saturated to obtain a first set of non-saturated results; and
performing a second automated scan of said array to detect said sample at a decreased sensitivity from said first scan to obtain a second set of non-saturated results.
2. The method of
3. The method of
4. The method of
determining if results from said first automated scan are saturated.
5. The method of
determining if results from said second automated scan are saturated.
6. The method of
performing a third automated scan with sensitivity decreased from said second scan.
7. The method of
determining if results from said third automated scan are saturated.
8. The method of
performing a third automated scan with sensitivity increased from said second scan, wherein said sensitivity is lower than that of said first scan.
9. The method of
determining if results from said third automated scan are saturated.
10. The method of
performing a fourth automated scan with a sensitivity increased from said third scan, wherein said sensitivity is lower than that of said first scan.
11. The method of
performing a fourth automated scan with a sensitivity decreased from said third scan, wherein said sensitivity is higher than that of said second scan.
12. The method of
13. The method of
transmitting results obtained by said optical scanning from a first location to a second location.
14. The method of
15. A method of optically scanning a sample in connection with a biopolymer array, said method comprising:
providing a biopolymer array;
performing a first automated scan of said array to detect said sample;
determining if any from said first scan are saturated; and terminating scanning if no results are saturated, or performing a second automated scan at a decreased sensitivity from said first scan if any results are saturated.
16. The method of
17. The method of
18. The method of
determining if results from said second automated scan are saturated.
19. The method of
performing a third automated scan with sensitivity decreased from said second scan.
20. The method of
determining if results from said third automated scan are saturated.
21. The method of
performing a third automated scan with sensitivity increased from said second scan, wherein said sensitivity is lower than that of said first scan.
22. The method of
determining if results from said third automated scan are saturated.
23. The method of
performing a fourth automated scan with a sensitivity increased from said third scan, wherein said sensitivity is lower than that of said first scan.
24. The method of
performing a fourth automated scan with a sensitivity decreased from said third scan, wherein said sensitivity is higher than that of said second scan.
25. The method of
26. The method of
transmitting results obtained by said optical scanning from a first location to a second location.
27. The method of
28. A system programmed to operate according to a method selected from a group of methods consisting of the optical scanning method of any one of claims 1-27.
29. The system of
30. A computer-readable medium embodying a program to direct a machine to perform a method selected from a group of methods consisting of the optical scanning method of any one of claims 1-27.
31. A computer-readable medium containing a program to generate data representing sample results, wherein said data is made by a method selected from a group of methods consisting of the optical scanning method of any one of claims 1-27.
This invention relates to optical scanners and, more particularly, to approaches for scanning low signal emission samples without photo-bleaching.
Optical scanners find use in performing detection for various experiments, assays and the like. They are often used in array analysis systems for detection of surface bound binding complexes in genomic and proteomic applications. In fluorescence optical scanners, fluorescence emitted from a sample that has been excited by a light signal is collected and detected.
Often, a user has little idea of the brightness of the fluorescence that will be emitted by a particular sample. Accordingly, the user does not know apriori how high or low to set the attenuation of an attenuator that controls the optical excitation signal power, that is, the signal power that reaches the sample. Further, the user does not know how high or low to set the gain of a detector that collects emitted fluorescence and produces a corresponding data signal.
Previously known optical system setup techniques include manually setting the sensitivity of the system where a user adjusts both the gain of the fluorescence detector and attenuation of the excitation light source. Typically, the user scans a sample in raster fashion to locate an element in the micro-array that is known to contain a concentration of a fluorophore that should produce a maximum fluorescence in response to the excitation signal. The user then re-scans the portion of the sample that contains this element and iteratively adjusts the sensitivity of the system until, in the judgment of the user, the corresponding data signal is sufficiently close to a maximum data signal value of the system. If the system has two channels, that is, produces excitation signals using two lasers of different wavelengths, the user re-scans the sample using the signal produced by the second laser and repeats the iterative, manual adjustment process to determine the appropriate sensitivity settings for the second channel. A user would further re-scan the sample for each additional channel.
The adjustment ranges of the attenuator or excitation source power and the detector are relatively large. Accordingly, manual adjustment of these components is time consuming, particularly since adjustment of either one of them may require a re-adjustment of the other. Thus, with manual scanning, the sample may be scanned many times to set the sensitivity of, or calibrate, the system. When multiple channels are used, more time is spent manually calibrating the system and the sample is scanned even more times, as discussed above.
If the excitation power and/or attenuator gain are set too low (i.e., detector sensitivity is too far reduced), the system may not accurately distinguish between different lower levels of emitted fluorescence. If the excitation signal power and/or detector gain are set too high, the system saturates. As stated in U.S. Pat. No. 6,078,390 to Bengtsson, assigned to General Scanning, Inc. (Watertown, Mass.), the general view is that such saturation results in a failure to make accurate measurements.
In response to such difficulties, the Bengtsson patent describes a scanning system and method of operation for automatically setting detection sensitivity. It employs an optical scanning system using a low-resolution scanning operation to automatically adjust the sensitivity of the system. The system performs a low-resolution scanning operation by scanning a line, automatically and iteratively setting the levels of excitation signal power and detector gain, skipping a plurality of lines and scanning a next line, adjusting the levels as appropriate, skipping a plurality of lines and scanning a next line, and so forth. After the system sensitivities have been set, the calibrated system then scans all the lines of the sample to collect data. The calibrated system thus scans for the first time the lines that were skipped during the low resolution �calibration� scanning operation.
For these skipped lines, photo-bleaching (i.e., weakening of fluorescent signal caused by exposure to excitation light) is avoided. With the other lines, however, the same problems encountered with manual scanning and tuning optical system attenuation or excitation system gain from photo-bleaching as a result of rescanning are encountered. The risk of damage to the sample is further increased when multiple channels are used.
The system of the present invention handles the reality of photobleaching in a different fashion. Rather than scanning samples and adjusting system limits to avoid photobleaching of unscanned samples during detection steps as in the Bengtsson patent, the present invention acquires valid data from some sample while the signal associated with other data is saturated. In this manner, low value signals are accurately detected with greatest accuracy. Rescanning areas producing more intense signals for detection after exposure resulting in photobleaching is not problematic since the relatively higher signal strength enables adequate data acquisition despite the deleterious effect of repeated light exposure.
The present invention handles issues associated with sensor saturation and photobleaching�not by seeking to eliminate each to the fullest extent possible, but by working around the problems they represent. Accordingly, the approach of the present invention is believed to offer improvement in detection accuracy in an elegant system. Furthermore, the invention accomplishes its goal in a highly time-efficient manner, saving resources in that regard. Additional benefits and advantages may also be apparent to those with skill in the art reviewing the subject disclosure.
The present invention is a maximum sensitivity optical scanning system. Generally, it operates by scanning a sample, such as in a DNA micro-array, at a setting calculated to result in signal saturation for some, but not all, available data. Preferably, scanning is accomplished at a maximum setting for a given scanning apparatus so that the weakest signal possibly detected may be observed. Sometimes complete signal saturation may result This condition indicates that no weak data is present. Whether or not data for weak signals is detected, one or more subsequent scans of the same area are taken at lower settings (in terms of detector attenuation and/or excitation light source gain) and data from at least those previously saturated regions is obtained.
This methodology works to preserve full signal sensitivity in taking data for the weakest signals. Another aspect of the invention provides for upward adjustment of scanning sensitivity, especially to obtain results when indication is received that a decreased sensitivity scan did not yield sufficiently strong results. Further, hardware associated with the methodology and programming optionally used to carry out the methods are part of the present invention. The system of the invention may incorporate known hardware as described herein or be otherwise implemented.
In describing the invention in greater detail than provided in the Summary of the Invention above, suitable hardware for use with the invention is first described, followed by the subject methodology or algorithm(s). Before the present invention is described in such detail, however, it is to be understood that this invention is not limited to particular variations set forth and may, of course, vary. Various changes may be made to the invention described and equivalents may be substituted without departing from the true spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process act(s) or step(s), to the objective(s), spirit or scope of the present invention. All such modifications are intended to be within the scope of the claims made herein.
Furthermore, where a range of values is provided, it is understood that every intervening value, between the upper and lower limit of that range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges as encompassed by the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either both of those included limits are also included in the invention. Also, it is contemplated that any optional feature of the inventive variations described herein may be set forth and claimed independently, or in combination with any one or more of the features described herein.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention, the preferred methods and materials are now described. All existing subject matter mentioned herein (e.g., publications, patents, patent applications and hardware) is incorporated by reference herein in its entirety. The referenced items are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the present invention is not entitled to antedate such material by virtue of prior invention.
It is noted that as used herein and in the appended claims, the singular forms �a�, �and�, �said� and �the� include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be so drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as �solely,� �only� and the like in connection with the recitation of claim elements or use of a �negative� limitation.
Turning now to the figures,
Scanning operations that use, for example, the excitation signal produced by the laser 4 are over a first channel, and scanning operations that use the excitation signal produced by the laser 6 are over a second channel. The term �channel� refers essentially to the system configured appropriately for the particular excitation signal, such as, for example, the selection of or conditioning of filters to pass or reject signals of the appropriate wavelengths and, as discussed in more detail below, the settings of appropriate levels of detector gain and attenuation levels. The drawing depicts two lasers, however, a single laser or a greater number of lasers may be included in the system with scanning operations over a corresponding number of channels, as appropriate for a particular application. Further, alternative excitation signal sources, such as, for example, xenon lamps or light-emitting-diodes, may be used instead of the lasers.
The variable attenuator 8 may be, for example, an addressable array of selected neutral fixed-density filters; a continuously variable neutral density filter; a plurality of polarizers that includes at least one rotatable polarizer; or a rotating polarization retarder placed in front of a polarizer, all of which are known in the art Alternatively, attenuator 8 may be omitted in favor of directly controlling the power of the excitation signal source. Such direct control may be accomplished using controller 14 to vary the voltage and/or amplitude of a signal dictating the operation of the excitation signal source. Typically, laser power has a generally linear relationship to input voltage that is easily determined and applied to control excitation source power output or gain.
As shown, mirror 16 may direct a beam 18 from laser 4 to a beam combiner 20, which transmits light at the wavelength produced by the laser 4 and reflects light at the wavelength produced by the laser 6. Beam combiner 20 may be, for example, a dichroic filter with thin-film interference coatings that transmit and reflect light at the respective wavelengths. It may be used to combine the beams from the two lasers into a single, collimated beam.
Shutters 22 and 24 may be provided and operated in a conventional manner to control the passing of light from the lasers to the beam combiner, such that at any given time light from only one laser reaches the beam combiner. Beam combiner 20 will then pass or reflect the beams, as appropriate. The beam combiner thus ensures that the beam from each laser travels along the same path to the sample 10.
A single or combined beam 18 may next pass through attenuator 8, resulting in an attenuated, collimated beam with a desired power level. Of course, the positions of any attenuator and beam combiner provided may be reversed, with the beam combiner passing, reflecting or combining the attenuated beams.
Preferably, a beam splitter 26 reflects the beam through a lens or lens assembly 28. The lens assembly focuses the beam and directs it to the sample 10. Beam splitter 26 also transmits light that lens assembly 28 collects from the sample 10 to variable gain detector 12.
Lens assembly 28, may be similar to a conventional microscope objective lens assembly, including multiple elements (not shown) that are mounted in a metal cell. Preferably, the lens assembly has a large working distance, a limited chromatic correction range and includes an objective lens with a high numerical aperture.
However configured (as would be a matter of routine design choice), lens assembly 28 focuses the combined, collimated beam that it receives to a spot that (for a micro-array scanner) is often within the range of approximately 1 to 20 microns in diameter measured at the full-width half-maximum point Of course, the lens assembly may produce a light spot with a larger diameter when the sample includes relatively large targets, or dots.
In response to the light spot, fluorescent chemical tags in the sample emit fluorescent light that is typically at a longer wavelength than the impinging light. Lens assembly 28 collects a portion of the emitted fluorescent light. It collimates and focuses the collected fluorescent light and directs it to a fluorescence emission wavelength discriminator 30. Discriminator 30 preferably operates in a conventional manner to filter the light from the sample. The discriminator, at any given time, passes light at a desired peak fluorescence wavelength and rejects light at other wavelengths, including light from the sample excitation sources that may be reflected from the sample 10. The discriminator may be, for example, an arrangement of emission filters that pass a desired narrow spectrum of fluorescent light. Alternatively, the discriminator may be a prism or grating that likewise passes the narrow spectrum of fluorescent light.
Discriminator 30 passes fluorescent light 32 to variable gain detector 12, which may be, for example, a photomultiplier tube (PMT) detector, a photodiode, a charge coupled device (CCD) or comprise other suitable hardware. Detector 12 preferably then sends data signals relating to the fluorescent light to the system controller 14, which records them for later use.
System 2 may include a plurality of discriminators in the path of the collimated fluorescent light, and a plurality of detectors. In which case, each discriminator will direct fluorescent light of a desired wavelength to an associated detector, and reject other wavelengths. The detectors can then each be matched specifically to a particular wavelength, and scanning operations involving multiple wavelengths can easily be performed simultaneously. Further, such a setup will avoid the need for shutters 22 and 24, or allow the system to be operated in synchronism to pass the multiple wavelengths.
Other hardware may also be used in connection with the present invention. For instance, the hardware described in U.S. Pat. Nos. 5,091,652; 5,294,799, U.S. patent application Ser. No. 09/846125, titled �Reading Multi-Featured Arrays� and 09/430214, titled �Interrogating Multi-Featured Arrays� each to Dorsel et al., a Hewlett-Packard G2500A system or an Agilent Microarray Scanner system may be used. It may be most preferred to use a system as shown in FIG. 2 and described in U.S. Pat. No. 6,320,196 to Dorsel, et al in connection with the present invention.
This hardware variation preferably includes two lasers, 48 and 50 which generate laser beams 52 and 54 of different wavelengths for exciting the two spots on sample substrate 34. The laser beams are combined by passing them through a first (dichroic) beam splitter 56 at a slight angle (the illustration is exaggerated for clarity) and reflected off a dichroic beam splitter 58. The laser beams are redirected by a folding mirror 60 and focused by an objective lens or lens assembly 62 onto the two separate spots 44 and 46. Fluorescent light emission from the spots is imaged back through objective lens 62 and fold mirror 60, through dichroic beam splitter 58 for imaging onto two multi-mode (or in other instances monomode) fibers 64 by a focusing lens or lens assembly 66. Fibers 64, one for each of the two spot's images, serve as a pinholes and also guide the fluorescent light emission to two respective detectors 40 and 42. In the preferred embodiment, the detectors comprise PMTs, but other devices such as an avalanche photo diode, a photo diode or a CCD may be employed. Detectors 40 and 42 may optionally contain a (compound) lens for imaging the light from the fiber onto the detector, as well as a filter for controlling the portion of the spectrum that is actually detected. The scanner may further include polarizers 68 each in a corresponding detection path.
In a typical arrangement, a �chip� for supporting a sample is used. The chip may be scanned by moving the scan lens and fold mirror assembly 70 back and forth across in one dimension, and slowly moving the chip in the orthogonal direction to produce a two dimensional scan. Alternately, relative motion of sample and confocal scanning spots can be achieved using a substrate carrier that is set into a spinning motion by a motor upon which the substrate is eccentrically mounted. The spindle carrier can be designed to carry more than one substrate in order to improve duty cycle. The spinning motion can be constant angular velocity or can be varied such that the linear velocity across the chip is independent of the radius of the scanning circle. (This radius may differ slightly for the two beams if they are offset other than tangentially, but this should typically only cause higher order effects.) While the spinning motion provides for a mostly tangential scanning motion, mostly radial scanning can be achieved by moving the motor with the substrate carrier, (e.g., using a translation stage). Preferably, the spinning motion would be the fast scanning motion and the translation the slow scanning motion such that the spots move across the substrate in a spiral trace.
A modification obvious to someone skilled in the art would be to slowly move the lens assembly radially. In this case, a polarizer is introduced into each of the detection paths, the crosstalk between which is to be reduced by polarization. The polarizer is then oriented in such a way as to maximize transmission of the (partially) polarized emission to be detected in the channel in question. For an eccentric (rectangular) sample substrate, the scanner will only receive signal intermittently. For a larger, concentric (round) substrate one would get a continuous signal while scanning an annular area on the substrate.
Furthermore, as with the system in
However configured, the design described in
Whatever the hardware used (whether that shown in
According to the methodology of the present invention, (whether using single or multiple light spots or multiple channels), a first automatic scan 72 of a site is made. When possible in view of the hardware selected, scanning for multiple channels and/or light spots is preferably accomplished simultaneously. However, with hardware configurations in which this is not possible, rather than running a full set of scans for each color or channel as described further below, the process will preferably proceed with highest intensity scans for each channel being made first, followed by any decreased sensitivity scans made in a turn-based manner (i.e., first a decision, adjustment or scan is made for one channel at a given point in the process followed by similar action for other channel(s) at that same point in the process, etc.). In systems with completely uncoupled channels, such that scanning in one channel does not photo-bleach or otherwise compromise the signal in other channels, it maybe acceptable to run through the whole process as described below for each channel sequentially. Whatever the case, for the sake of clarity as the invention is explained further below, it will be made in the context of a single channel system, though the plurality of scans called for in connection with multiple channels may be implied.
The first scan (or set of scans) will typically be at full-power scan (i.e., a scan taken at the limits of detector attenuation or gain and excitation source power). In this way, the scan is taken at the maximum system sensitivity.
It is possible, however, that the initial scan setting might be set below the system sensitivity limit. When it is known apriori that no weak signals are present or likely to be present (for instance by related experiments), excitation source gain or attenuation and/or detector gain may be set lower so the results of the first automated scan are, hopefully, not completely saturated. Generally, the initial scan made automatically by the system is to be the highest sensitivity level scan�and thus, the �maximum� sensitivity scan�made according to the inventive algorithm.
After the initial scan, system logic preferably performs a check 74 to determine if any of the scanned area resulted in saturated readings. If no �results� are saturated, then the first scan is the only scan and the scanning process ends as illustrated. At the end point, scanning ceases and the data obtained is processed and/or displayed as typically done in the art.
By �results,� it is preferably meant information from statistically relevant pixel(s). When scanning is accomplished, a PMT or CCD image obtained is represented by a number of pixels. Some of those pixels may show up as saturated regardless of the amount of sample in the region being scanned. A smudge, lint or other contaminant may be the cause, as may faulty hardware. Such readings are typically discarded upon operation of analytical techniques commonly practiced in the optical acquisition of data before check 74. Examples of such techniques are described in John C. Russ, �The Image Processing Handbook,� Chapter 3, 3rd Edition (1998), and/or embodied in optical scanner instruments such as the AGILENT MICROARRAY SCANNER (product no. G2565AA) available from Agilent Technologies, Palo Alto, Calif. and biological image-processing tool kits such as Biolmage, see the �www� site of �mih.unibas.ch/Bioimage/Consortium/BItools. html.� Pixels that are not statistically selected or otherwise modified�as in median filtering�may, however be counted as �results� in less preferred variations of the invention.
If a check procedure is included and some of the results thus defined are saturated, then system sensitivity is decreased as shown at step 76. Even without checking, however, the system could proceed to decrease sensitivity for another scan. Still, without check 74, significant advantages in terms of time savings and scanning accuracy may be lost.
The manner in which system sensitivity is decreased may vary. In general, the decrease may be related in some way to the amount or extent of result saturation. More complete result saturation will indicate the need for a more dramatic decrease in sensitivity. Either one or both of the gain of the light source(s) or detector(s) (e.g., PMT, CCD, etc.) may be changed to allow rescanning at a reduced scale factor. The decrease in sensitivity applied to the system may be a drop in light source power reaching the sample, detector gain or both. In the case of PMT sensitivity, while the relation between applied voltage and gain is nonlinear, the extent of change may be predicted utilizing the power law published by hardware vendors with empirically determined coefficients to make an estimate or by an iterative approach in testing gain obtained in varying voltage against expected results. Typically, sensitivity will be reduced by a scale factor between about 2 and 10 times. Still, more drastic modifications are possible, even if some commercially available scanning systems are not set up to accommodate such input.
Once system sensitivity is adjusted, a decreased sensitivity scan 78 is performed. One option is for the process to simply terminate at this point. Another option is that a second decision 74 is made resulting in process termination if no saturated results are detected, or proceeding again to decrease resolution and perform another decreased resolution scan.
In the event sufficient or useable results are not produced, a system sensitivity increase 82 is made. An increased sensitivity scan 84 follows. The process may end there or next proceed to check for usable results again.
As with the manner in which system sensitivity can be decreased, the manner in which it is increased may vary. Generally, the magnitude of the increase should be less than decrease that produced no usable results. The increase in system sensitivity may be on the order of about one-half of the preceding system sensitivity decrease.
Of course, upon review of either approach taught in
For a second and any subsequent reduced-sensitivity or increased sensitivity scans, correction for the results of the (known, calibrated) effects of photobleaching from preceding scans (at least the first, maximum sensitivity scan) is preferably applied. What is more, additional steps or actions may be made in connection with or intermediate to the actions disclosed. However, for the sake of speed and economy, the invention is preferably practiced with only with such primary activities as shown in the figures and any associated data correction and/or filtering.
The present invention is preferably used in conjunction with arrays of polymeric binding agents spotted onto a surface�possibly by inkjet printheads, by pin spotting or otherwise. The arrays will usually include at least two distinct polymers that differ by monomeric sequence immobilized on, e.g., covalently or non-covalently attached to, different and known locations on the substrate surface. Each distinct polymeric sequence of the array is typically present as a composition of multiple copies of the polymer on the substrate surface, e.g. as a spot on the surface of the substrate. The number of distinct polymeric sequences, and hence spots or similar structures, present on the array may vary, but is generally at least 2, usually at least 5 and more usually at least 10, where the number of different spots on the array may be as a high as 50, 100, 500, 1000, 10,000 or higher, depending on the intended use of the array. The spots of distinct polymers present on the array surface are generally present as a pattern, where the pattern may be in the form of organized rows and columns of spots, e.g. a grid of spots, across the substrate surface, a series of curvilinear rows across the substrate surface, e.g. a series of concentric circles or semicircles of spots, and the like. The density of spots present on the array surface may vary, but will generally be at least about 10 and usually at least about 100 spots/cm2, where the density may be as high as 106 or higher, but will generally not exceed about 105 spots/cm2.
In the broadest sense, the preferred arrays are arrays of polymeric binding agents, where the polymeric binding agents may be any of: peptides, proteins, nucleic acids, polysaccharides, synthetic mimetics of such biopolymeric binding agents, etc. In many embodiments of interest, the arrays are arrays of nucleic acids, including oligonucleotides, polynucleotides, cDNAs, mRNAs, synthetic mimetics thereof, and the like. Where the arrays are arrays of nucleic acids, the nucleic acids may be covalently attached to the arrays at any point along the nucleic acid chain, but are generally attached at one of their termini, e.g. the 3′ or 5′ terminus. Sometimes, the arrays are arrays of polypeptides, e.g., proteins or fragments thereof.
The subject arrays find use in a variety applications, where such applications are generally analyte detection applications in which the presence of a particular analyte in a given sample may be detected qualitatively or quantitatively. Protocols for carrying out such assays are well known to those of skill in the art and need not be described in great detail here. Generally, the sample suspected of comprising the analyte of interest is contacted with an array produced according to the subject methods under conditions sufficient for the analyte to bind to its respective binding pair member that is present on the array. Thus, if the analyte of interest is present in the sample, it binds to the array at the site of its complementary binding member and a complex is formed on the array surface.
The presence of this binding complex on the array surface is detected according to the present invention by optical means, e.g. through the use of fluorescent label present on the analyte, detecting chemiluminescent or electroluminescent labels or otherwise. The presence of the analyte in the sample is then deduced from the detection of binding complexes on the substrate surface.
Specific analyte detection applications of interest include hybridization assays in which the nucleic acid arrays of the subject invention are employed. In these assays, a sample of target nucleic acids is first prepared, where preparation may include labeling of the target nucleic acids with a label, e.g. a member of signal producing system.
Following sample preparation, the sample is contacted with the array under hybridization conditions, whereby complexes are formed between target nucleic acids that are complementary to probe sequences attached to the array surface. The presence of hybridized complexes is then detected. Specific hybridization assays of interest which may be practiced using the subject arrays include: gene discovery assays, differential gene expression analysis assays; nucleic acid sequencing assays, and the like. Patents and patent applications describing methods of using arrays in various applications include: 5,143,854; 5,288,644; 5,324,633; 5,432,049; 5,470,710; 5,492,806; 5,503,980; 5,510,270; 5,525,464; 5,547,839; 5,580,732; 5,661,028; 5,800,992; the disclosures of which are herein incorporated by reference.
Where the arrays are arrays of polypeptide binding agents, e.g., protein arrays, specific applications of interest include analyte detection/proteomics applications, including those described in: 4,591,570; 5,171,695; 5,436,170; 5,486,452; 5,532,128; and 6,197,599; the disclosures of which are herein incorporated by reference; as well as published PCT application Nos. WO 99/39210; WO 00/04832; WO 00/04389; WO 00/04390; WO 00/54046; WO 00/63701; WO 01/14425; and WO 01/40803; the disclosures of the United States priority documents of which are herein incorporated by reference.
The subject methods may include an act of transmitting data or results from at least one of the detecting and/or deriving acts, to a remote location. By �remote location,� it is meant a location other than the location at which the array is present and hybridization occurs. For example, a remote location could be another location (e.g., office, lab, etc.) in the same city, another location in a different city, another location in a different state, another location in a different country, etc. As such, when one item is indicated as being �remote� from another, what is meant is that the two items are at least in different buildings, and may be at least one mile, ten miles, or at least one hundred miles apart. �Communicating� information means transmitting the data representing that information as electrical signals over a suitable communication channel (for example, a private or public network). �Forwarding� an item refers to any means of getting that item from one location to the next, whether by physically transporting that item or otherwise (where that is possible) and includes, at least in the case of data, physically transporting a medium carrying the data or communicating the data. The data may be transmitted to the remote location for further evaluation and/or use. Any convenient telecommunications means may be employed for transmitting the data, e.g. facsimile, modem, internet, etc. Results according to the present invention (including further processing such as accounting for photobleaching or not) may be forwarded (such as by communication) to a remote location if desired, and received there for further use (such as processing or further processing).
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How much does this table weigh?
I bought this table to use it for additional table space on Thanksgiving for the food. It didn't take up too much room yet was perfect for placing the salad and extra bowls of food that did not fit on the table. It is sturdy and when I put the table cloth on it, it was perfect for what I needed it for. This was a great value. The item was delivered quickly and in perfect condition. Very happy with my purchase....
Cosco 20x48 ABS Top Folding Table
This plastic-top folding table from Cosco has a sturdy steel frame, so it will hold up to heavy use, but because it has a plastic top, it's lightweight and easy to tote from place to place. The legs fold up, so storage is no problem.
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ITEM#: 15388202This Cosco table is designed for indoor or light outdoor use. It is stylish, strong, and easy to clean. This table saves space and time with table legs that fold in to make for easy storage. Features a heavy duty steel frame and steel legs.
- Brand: Cosco
- Model: 0014449BLK1E
- Materials: Steel
- Color: Black
- Dimensions: 28 inches high x 48 inches wide x 20 inches deep
- Shape: Rectangle
- Plastic top allows for indoor and light outdoor use
- Easy to carry
- Folds flat 1.5 inches wide for easy storage
- Leg tips protect floor surfaces
|Black Finish
|Furniture Frame Material
|Furniture Pattern
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|Manufacturer Warranty
|1 year Limited Manufacturer
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Ok for the price
It's quite lightweight - I wanted it as a crafting table, but it is so light that any bump makes it rattle.Read More
Folding Table
I bought this table to use it for additional table space on Thanksgiving for the food. It didn't take up too much room yet was perfect for placing the salad and extra bowls of food that did not fit on the table. It is sturdy and when I put the table cloth on it, it was perfect for what I needed it for. This was a great value. The item was delivered quickly and in perfect condition. Very happy with my purchase.Read More
This is the second table delivered to me - the first one was in bad shape - top not glued down, rough edges. The second one is somewhat better, but is very wobbly and I am not particularly pleased with it. However, I do not want the hassle of sending it back again. I will keep it, but would not order this item again.Read More
great lightweight tables
I purchased these tables to use at Craft Fair event. They are great lightweight tables and very easy to bring with me and store in the car. They are very lightweight and I would think would not be that great if you had heavier items to put on them. The items I put on them for my craft fairs are very lightweight so I have not experienced any problems. The covering is nice and I like the overall black design. If you're looking for something lightweight, quick to stand up and take down and I would think would fit across most midsize back seats for transport this is the one for you. I would not use it for at a dining room seating though as I would question the table tops ability to hold heavy items for great lengths of time.Read More
Great table for the money.
The table is compact and very easy to store. It's also light and easy to move.Read More
tables bowing
While the table is a good value-sturdy and attractive; after only one month they started bowing in at the center. There is nothing stored on them. They are used on average 2 hours per day, 3 days were week and for some reason some (not all) of them are caving in. I would like to contact the company and see if there is a history or if they can be exchanged but have no contact info.Read More
Questions & Answers
Hello macclan4, the table weighs about 16 lbs. Thank you for shopping with us.
.extremely lightweight I would guess 5 to 10 pounds at most
hello, I would like to know if the table top is flat and smooth enough to write on? some plastic tips are slightly bumpy.
Hello, vamwam. Hope your day is going well. This item is fine for writing on but not 100% smooth. There is a slight texture but for general purposes it's usable as a work space. Thank you for shopping with us and please feel free to contact us again with any further questions.
We used ours for our tabletop barbeque. It's not been sturdy. We used it for a puzzle at Christmas and it worked out. Although we're careful about not leaning on it for fear of collapsing. I wouldn't buy this again .
I want to put a sewing machine and various accessories on this table. What is the maximum amount of weight it will safely hold?
Hello, kellen46. Hope your day is going well. The maximum evenly distributed load for this table is 100 lbs. Thank you for shopping with us and please feel free to contact us again with any further questions.
While they are reputed to be able to hold 100 lbs mine are bowing in at the center with nothing stored on them.
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Steak With Corn and Tomatoes
Savory Sight: Steak With Corn and Tomatoes
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We're not the only ones who are eagerly awaiting Summer for its lively produce; so is lauren.Can't wait for summer with corn and tomatoes!
Have your own tasty dinner you'd like to tempt us with? Then be sure to share your food photos in our community or by starting your own blog. If you're on Instagram, then chime in on the conversation with the hashtag #savorysight.
Read More Savory Sights
mzgigi 2 years
where's the recipe?
Link Love
- 100+ of the best DIY gifts ever — POPSUGAR Smart Living
- 24 signs that show you love food more than people — BuzzFeed Food
- Why you should get excited about chopped liver — Food52
- Make the most out of asparagus in Spring with this flatbread recipe — PureWow
- If you're sweet on citrus desserts, these recipes are for you — Southern Living
- A visual guide to cooking with herbs — HuffPost Taste


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Comfort Suites Innsbrook
4051 Innslake Dr
Glen Allen, VA 23060-3366
Phone: (804) 217-9200
Fax: (804) 217-9205
Canal Walk
14th and Dock Streets
Richmond, VA 23219
+1 804 788 6466
Mo to Su from 12:00 AM to 12:00 AMBy boat or by foot the Richmond Canal Walk is a beautiful and educational experience. Take a guided tour or learn about the site on your own. Trek through wooden steps, cobbled streets, and dirt trails - you certainly won't be bored with all of the pathways this walk offers. Brass disks embedded in the sidewalk, maps, photos and artifacts note historical events and people associated with the canals and locks.
Earn valuable points toward free nights.


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Recent Customer Reviews
Love my wreath. it was received in good condition and it lasted throughout the christmas seasonRead More
Kate B.
Light weight. Seems solidly constructed. Vibrant colors but will have to see how they hold up on the ocean.Read More
Linda J.
Very sturdy, great color, easy to assemble. Would defintely recommend!Read More
Brittney P.
Easy to stain and put together. The size was perfect for the area. Would recommend this product.
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Karla S.
Superior quality and fine craftsmanship. I would highly recommend the purchase of this product from ATG. It was an enjoyable experience and went without a hitch. Highly satisfied.
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Margo L. M.
Great Product. Very Happy with this windmill.Read More
Cindy H.
The windmill was well made. The windmill does not freely turn. With some adjustments I will install a bearing so that it will spin.Read More
David H.
Really great-looking table and great for the money. I wish it came in more colors.Read More
Rosemary J.
I previously reviewed the bench by the same company "Shine Company" and I'm equally pleased with the rectangular side table. Any questions I had, ATG customer service provided the answers.Read More
Rosanne G.
The quality, workmanship, finish and size is perfect. We purchased the natural so we could finish as desired and the wood is ready for whatever one wants. We confidently recommend this bench and side table.Read More
Rosanne G.
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ATG Stores.com is the best place to shop for Shine Company products
For over 30 years, Shine Company has been designing and producing a variety of solid wood outdoor furniture and garden accessories. Shine's mission is to provide for customers seeking quality wood products that are both charming and functional.
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Feel like home at ringtone download service Audiko.net, where it's possible to search for epic ringtones for your Android smartphone. Today we have a ringtone of "Elegy" by Jethro Tull as well as 491 other ringtones by Jethro Tull. "Elegy" is a top-rated song from the "A Classic Case: The London Symphony Orchestra Plays the Musi" album that was recorded and mixed by Jethro Tull at their label together with other 6 tracks. You can listen to all 8 Jethro Tull – "Elegy" ringtone variations available here and download it for free right to your phone. You can also choose from other 26 albums produced by Jethro Tull for all time. "Elegy" is referred to Progressive Rock, and Audiko offer a huge amount of Progressive Rock tracks absolutely for free. And finally, we have collected lyrics for this track and you can view it here as well. So feel free to browse, listen, download ringtones at Audiko.net. Make your phone sound cool!
Jethro Tull Elegy Ringtone
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Albums by Jethro Tull
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11 Apr 2005
The Jethro Tull Christmas Album
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30 Sep 2003
Top ringtones by Jethro Tull
New ringtones
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- Biggy Jiggy Mixtapes - 04 Young Jeezy Me Ok (Remix) (Feat Gucci Mane)
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- veysel - Futura (feat. Olexesh)
- MCFC - Boys in Blue
- Marina and the Diamonds - Blue
- Linkin Park - A Light That Never Comes DR11
- Hardsoul & Dirty Freek - Just Another Face (feat. Bridgette)


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New York Rangers
News about the New York Rangers, including commentary and archival articles published in The New York Times.
Henrik Lundqvist Back on Track as Rangers Top the Jets
After sitting out 25 games with a neck injury, the Rangers goalie made 32 saves for a road win against Winnipeg.
April 1, 2015, Wednesday
Derick Brassard and Mats Zuccarello Ignite the Rangers' Line
Brassard at center and Zuccarello on the right wing have been the team's most consistent line for the past two seasons, regardless of who plays left wing.
March 31, 2015, Tuesday
Rangers Fall Flat at the End of a Lost Weekend
Alex Ovechkin and Jason Chimera each scored twice to lead the Washington Capitals past the lackluster Rangers on Sunday at the Garden.
March 30, 2015, Monday
Henrik Lundqvist's Return to Rangers Is Ruined by Bruins
The Rangers' goalie was peppered with shots in his return from a throat injury and the Bruins scored four times in the first 26 minutes, taking advantage of several defensive lapses.
March 29, 2015, Sunday
Madison Square Garden to Split Into Two Companies
The company confirmed Friday that it would spin off MSG's live sports and entertainment businesses from its media business.
March 28, 2015, Saturday
Madison Square Garden Confirms Plan to Split in Two
One company will include the sports teams, the namesake arena and Radio City Music Hall, while the other will have regional sports networks.
March 28, 2015, Saturday
Rangers Clinch Playoff Spot With Rout of Senators
Chris Kreider had two goals and an assist, and the Rangers clinched a playoff spot with a 5-1 victory over the host Ottawa Senators.
March 27, 2015, Friday
Rangers' Lundqvist Will Be Backup Thursday as He Nears Return
Lundqvist, sidelined since Feb. 2, will be back on the Rangers' bench Thursday and is expected to start at least one game this weekend.
March 26, 2015, Thursday
Kings Awake From Slumber Against Rangers
In a rematch of the Stanley Cup finals, Los Angeles looked more like the team that dispatched the Rangers last spring than the one that has struggled for months to earn a postseason bid.
March 25, 2015, Wednesday
Rangers' Henrik Lundqvist Expects to Play This Weekend
The goaltender took shots for the first time since he was placed on injured reserve with a vascular injury in his neck Feb. 6 and said he hoped to return to game action this weekend.
March 25, 2015, Wednesday
Key Moments From the Sports Summer of 1994
Twenty years later, revisit the most memorable moments from the sports summer of 1994, with archival photography and video recollections from Times reporters and editors on the Knicks, Rangers, World Cup and more.
Kings Capture Cup at Home
The Kings rallied in the third period to force overtime and scored in the second extra period to beat the Rangers in Game 5 and win the Stanley Cup.
Recalling 1994 in Sports
The sports year of 1994 included two New York teams chasing a title, a World Cup, the O.J. Simpson scandal and a baseball strike. Times reporters and editors reflect on these events from 20 years ago.
1994 in Sports: Messier's Guarantee
Mark Messier guaranteed a victory as the Rangers faced elimination in the Eastern Conference finals. The Times's Bill Pennington, George Vecsey and Jay Schreiber reflect.
1994 in Sports: Rangers Win the Cup
The Times's Bill Pennington reflects on the Rangers winning Game 7 of the Stanley Cup finals against the Vancouver Canucks.
Rss Feeds On New York Rangers
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Get Alerts On New York Rangers
Receive My Alerts e-mails on topics covered on this page.More Alerts >>


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Publisher's Description+
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What's new in this version:
Version 3.5 updates the digital signature.


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Sri Lanka clings on in Lord's thriller
Nuwan Pradeep survived the final five balls of the match as Sri Lanka clung on for a thrilling draw with England in the first Test at Lord's on Monday (June 16). They will go to Headingley on Friday all square with one to play.The expected overnight declaration set the day up as one in which Sri Lanka required 390 runs and England required 10 wickets. Or, much more likely, neither requirement would be met and the docile pitch could be declared the winner. Though James Anderson beat the bat under cloudy skies during an impressive new ball spell, it was Stuart Broad who got the breakthrough, inducing Dimuth Karunaratne to pop one up to short leg where Sam Robson took a sharp… Read More →


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These bakers found 8 creative ways to put the little bottoms on display this holiday.
Creative Party Snacks: 7 Savory "Cookies"
This incredibly quick and easy dish from New Orleans chef Donald Link's Down South cookbook features tuna steaks poached until rare in a bright, buttery sauce that's infused with tangy lime and kumquats, spicy jalapeños, and fresh mint. Doubled or tripled, it would make an excellent dinner-party dish.
Recipe: Poached Tuna with Kumquats and Jalapeño
The double dose of lemon in these simple, delicious British puddings gives them an extra hit of delicious tangy flavor.
Recipe: Lemon Puddings with Granny Smith Apple Compote


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Achetez vos crampons et maillots de Rugby chez Lovell Rugby
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First impressions count and so should the first layer in contact with your body. Just as you would take time to choose a piece of rugby kit or rugby boots to improve your performance, the base layer of any rugby player's kit is equally as important in improving performance and protection. In simple terms base layer clothing has been specifically designed to draw moisture away from the body whilst supporting muscles during any level of activity. The resulting effect is greater comfort combined to maximise your performance in any environment.
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Athletes of a fashion conscious nature will be delighted by the range of short and long sleeve base layer shirts from Under Armour and Canterbury. Perfect for combining with any additional layers of clothing or to simply stand out on the pitch.


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The Crap We Missed – Wednesday 4.17.13
29 of 34
Amy Childs at The Virgin London Marathon. (April 17, 2013)
Photo: Bauer-Griffin, Fame/Flynet, Getty, INFdaily, Pacific Coast News, Splash News, WENN
Tags: Amy Childs
Fart runner?
I know, right? WTF?
"Fuck! I just got a shot from behind, this could be anybody. I wonder who I can con into buying this from me?"


text: Every little girl is God s precious princess. This Bible includes the complete New International Reader s Version (NIrV) the NIV for kids Twelve adorable and colorful tip-in pages will delight girls with Bible verses that assure her she is a child of the King. Features include: Presentation page for gift giving and personalization Complete text of the New International Reader s Version (NIrV) of the Bible the NIV for kids Twelve colorful pages of illustrations of princesses along with Bible verses that describe characteristics of a princess and list the princesses and queens mentioned in the Bible Dictionary for explaining difficult words List of 150 famous Bible stories for suggested reading Easy-to-read type size"


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Ordered an item that competitors seem to have a problem getting. MJM got it to me in time for my next show. This was my first order - will not be my...Read More ->
I contacted customer service late on a Friday and I figured I'd hear back on the next Monday but instead I had a reply on Saturday! They addressed...Read More ->


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VIEWS: 67 PAGES: 1 CATEGORY: iPhone POSTED ON: 6/27/2011
An Apple iPhone 3G busted screen sounds like a serious problem, but when you have our repair service such as ours to hand, it is only an inconvenience
Apple iPhone 3G Busted Screen Accidents occur and it isn't surprising when someone drops an Apple iPhone 3G or an object is dropped upon it, and the screen is broken. An Apple iPhone 3G busted screen sounds like a serious problem, but when you have our repair service such as ours to hand, it is only an inconvenience! Our Apple iPhone 3G Screen repair is for when the image behind the front glass is black or white, else the image is distorted or has parts missing. Otherwise you might need the iPhone 3G Front Glass / Digitiser Repair instead. We can easily fix your Apple iPhone screen and all it takes are three simple steps: Step 1 – Order your Apple iPhone 3G screen repair by clicking here. Step 2 – Package and post your damaged Apple iPhone 3G to our Banbury repair centre. Step 3 – Within forty-eights or less we will fix your Apple iPhone 3G screen. Three simple steps an before you know it, your Apple iPhone 3G is repaired and back with you. We guarantee to fix your Apple iPhone 3G screen. Plus we already have the replacement 3G screens onsite ready and waiting for your damaged iPhone 3G. We guarantee to repair your broken Apple iPhone 3G screen in 49 hours or less. We've been fixing Apple iPhones since September 2007 and as you can image we have repaired a lot of iPhones through the years. Our Apple iPhone repair service has been tailored to quickly turnaround the repairs, but also still providing a quality iPhone repair service. For instance when we receive your faulty Apple iPhone 3G we send you an email, plus we will notify you when we send the repaired Apple iPhone 3G back to you. "Thanks for fixing my phone so quickly, it's a great service…" - S kavanagh Don't opt for expensive high street repair services, use our professional iPhone hardware repair service to mend your device. We specialise in Apple iPhone repairs, unlike many of our competitors, which has enabled us to gain the experience that many of our competitors lack. It is not even worth considering trying to buy the replacement 3G screen and repair the iPhone yourself. Not only is it unsafe to attempt the repair yourself, but the procedure is complex and you could cause further damaged to your Apple iPhone 3G. If you have any questions please do not hesitate call us on 0845 519 6356 or use our contact form. Have a busted Apple iPhone 3G Screen? Click here to order your repair.
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orange flowered tree with 'half' fruit
boquetebonnieJune 20, 2007
At the Hotel Gran Nacional in David, Panama yesterday, I observed a medium-sized tree with brilliant orange single flowers and a lemon-sized "fruit" that looked as though it had been cut in half. I've searched my tropical gardening books, but can't identify it. Can anyone help? (I simply must have one for my garden in Boquete.)


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At an event marking the 15th anniversary of the original Digimon Adventure series in Odaiba, Tokyo on 1 August, an announcement was made declaring a brand new chapter in the human-monster buddy saga will bring back the original characters.
Along with the announcement came a special anniversary website with some Digi-Eggs containing details of the new series. However, no matter how much you rubbed those suckers nothing came out – until now.
The hatchling information included a brief rundown of the staff and a first look at all eight of the DigiDestined more evolved…I mean grown up.


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ON-AIR Weekdays 10am-2pm WPGC 95.5/ Philanthropist/ Ciroc Girl/ Direct Access Ent. Correspondent/ Bosnian Sweetheart
Instagram: SunniOnAir
Face Tags
I was the sexy Dorothy from the Wizard of Oz and my HOTTT showgirl :):)
T-Pain is so disrespectful for this shit! Wearing a shirt w/Cassie's nude photo on it.. I hate men like this!!!
My bedroom window view is amazing! Now.. if this was only NYC or Chicago
Party like a ROCKSTAR! Lets goooo! C u at Crave!!! Out the door in 5 4 3 2 1...
Wearing my Fo-Hawk tonight LOL...see you at Cloud 9!
Researching all of these apts/condos in Miami, this is the view I want to wake up to...ahhh beautiful
The Friday Night at Cloud 9 Crew! Come party with us every Friday! Lookin' good fellas :):):)
OMG @ESwilley remember this? lol last year.. young boi Amir
Not sure if the blue eye shadow is in but I like it and I'm rockin' it tonight! Lets goooo Cloud 9!!
Sorry @Every1LuvsJaime but I'm completely jackin' your style tonight! :) its sooo cute I couldn't help it
I got all of of my nail polishes organized by brand/color lol
Maaaan I couldn't help myself, had to make it again.. :) Crab/Cucumber Sushi.. I'm getting goooood at this
Yes I bought a snuggie! 2 of them! And I looooove it! :):);)
Yaaay! Shrimp tempura! Home-made! Yummy!
Waiting for @EvetteH to pick me up!!! Hurry up!!! This is @djmobeatz look! lmao
This is why I'll b hungry by 11:30 tonight! There is nothing here in the fridge smh
Healthy lunch! Tilapia, with mushrooms and edamame, skinny bish here I come! lol
My bros.. Chuck, @chosen214 and @stephenhendrix1 Cute! Karma Sutra Ent. biiiiatches! lol
My babies! It was hard as hell taking this picture lol... love 'em!!!!
Was wondering whats all the noise outside my bldg, new movie being filmed downtown
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5 Packages starred by zenanyoo
- fs-extra fs-extra contains methods that aren't included in the vanilla Node.js fs package. Such as mkdir -p, cp -r, and rm -rf.
- parseuri Method that parses a URI and returns an array of its components
- underscore JavaScript's functional programming helper library.
- useragent Fastest, most accurate & effecient user agent string parser, uses Browserscope's research for parsing
- walk A node port of python's os.walk


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Dining Table needing Chairs
monicajohnJune 10, 2008
I have a large wooden table that is 29.5" in height. The apron is 5.5" leaving only 24" for clearance to the ground. I am having trouble finding comfortable chairs with a low enough seat height that will fit under the apron. Can chair legs be shortened to a desired height if they are just basic chair legs?
Yes. I do this regularly with barstools and countertop stools. The standard chair seat height is 18"+- Don't vary too far from this or they will not feel comfortable to average sized people. That would give you six inches from chair seat to bottom of apron, which, IIRC, is the minimum.


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PD.com: We have 73 Virgins!
This section allows you to view all posts made by this member. Note that you can only see posts made in areas you currently have access to.
Calling a daemon a service is a matter of semantics and is a naming choice made by Windows in particular, not by proprietary operating systems in general.
Mind as a computer metaphors are seriously fucking annoying because prats like you get too caught up in making a 1 to 1 mapping between elements of either system and stress the comparison to the point of uselessness.
So I've just now realized
Somewhere down the line
I earned my table place
4 stars at my own pace
Thus I truly Deserved It!
Gosh, I always wanted to be a Hermetic terrorist ever since I first heard of Hermes.
That kid was my favorite Greek god when I was growing up.
Troublemaker, entrepreneur, awesome kicks, marvelous lyre, all that AND a pile of rocks!
Gonna set to work writing today. Left my Sunday open for the purpose.
First project is the GRIN bit EOC started up. Never leave home without it!
I like that guy. His articles on fallacies are the bomb.
Stock buybacks were once considered a form of illegal stock manipulation, until 1982, when President Ronald Reagans Securities and Exchange Commission chair John Shad (a former Wall Street CEO) loosened the rules.
Even the sky is symbolically perfect. Overcast and grey as hell.
Youremployers are criminals, dude. Wow.
Trust me, it's really NOT just his. Or the UK.
And really, really, not a minority either.
ETA - Well that sounds condescending as fuck. Not the intention/tone.
My adhd ridden friends won't sit still for more than 30 seconds to understand anything besides QWER and league tier lists.
What's a good quick pitch of it.
I've tried before but they keep saying that it's "le so randum" and only liked by scene girls or 2deep4you type people.
Like how do I make a reasonable pitch, especially with how it is now a relatively unheard of thing.


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News & Features
Alphabetical Guide to California Values
October 18, 2002
News & Features : Unfiltered
Best of Unfiltered 2008
The most popular stories of the past year indicate that with all that happened in 2008, we really just wanted to be distracted by a pretty face
December 31, 2008
News & Features : Unfiltered
Unfiltered: Playboy Bunnies Wrap Themselves Around Wine Bottles
Plus, drinking wine to save the planet, Italians turn water into wine, French temperance extends to the postal system and a wine tribute to the late Bobby Fetzer
October 8, 2008
News & Features : Retrospective / Vertical
1975 California Cabernet Retrospective: The Past Still Lives
Amid an aging class of wines, a couple of stars
June 16, 2005
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Pezzi King Winery Hires Former Opus One Winemaker
April 8, 2000
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