text:
Hotel Goldcity Tourism Complex(5 stars)Kargicak - Alanya 07435 Kargicak View on map
Show on map:
- Hotel
- Things to do and see
Hotel review overview
The most helpful positive review
read more Excellent, Very good reviews
We got exactly what we had booked Family suite ( 101square meter) … read hotel review
The most helpful negative review
read more Acceptable, Poor, Terrible reviews
The hotel was huge-as others have described 16 sticks-divided into west, north, east and south wing -16 floor was a bar and a sushi restaurant against an extra charge - the outside rum were many bungalows - shuttle service available - cleanliness leaves something to be desired - all-inclusive was all right - drinks also cocktails were almost all uneatable-no brand name drinks, was-only a büfettrestaurand, far from enough - very lots of russians the partly … read hotel review
Overall review
- Recommended hotel
- Couples
- Beach holiday
- March 2015
The hotel is welcoming and comfortable.staff very accommadating and go out of theire way to help.the restaurant that was open was light and both food and service exelent.we would return again read hotel review
Fantastic holiday at Goldcity Hotel Alanya
- Recommended hotel
- Couples
- Beach holiday
- March 2015
We are so delighted to say that we enjoyed every moment in Gold city Alanya. the room we got was truly a 5 star room with jacuzzi, we arranged for a jeep safari that took us to a breathtaking view of the taurius mountain! It was full of fun and adventure, also arrange a tour to dim cave, which was also amazing! Thanks to Ahmet P. for helping out with organising our day trips- He was truly professional and honest in his recommendation. The staff at the hotel were very friendly and helpful. Would highly recommend. read hotel review
Very nice stay
- Recommended hotel
- Couples
- Skiing and winter sports
- March 2015
The food is very nice always fresh plenty of choice although sometimes not very hot The rooms are very nice we had a complimentary upgrade to a suite with a large balcony, with the sun every morning on the balcony. Even though the hotel was not full the staff gave you their full attention, they were very pleasant and happy to assist you in any way they could. Ahmet the british rep was in the hotel daily to assist ic we needed him. We booked atour with him to the caves and the castle a full day tour with an ... english speakimg guide who was very informative. Ahmet sorted out our transfer times for pur departure. I would recommend anyone staying here to speak to him he is very helpful and informative and his english is very good read hotel review
Mr Stanislavs O. and Ms Agneta O.
- Recommended hotel
- Couples
- Beach holiday
- March 2015
I can left only good fedback. You got very palet staff, our room was very good, and we had very good Service from, tatjana,reception supervisor huseyin ,elvina, Massage stafff, bebeto BEBETO and gokce Guest relation micith read hotel review
Family holiday
- Recommended hotel
- Families
- Beach holiday
- March 2015
It was really good experience and good customer service and guide by ahmet paca who works for tourist company Hotel was really good and the staff wer extremely helpful and the rooms wer nice and clean we loved the experince amd will defenitly come back again Quad biking was an extremely good experience its a experience everyoone should try read hotel review
A week relaxing
- Recommended hotel
- Couples
- Beach holiday
- March 2015
A speedy check in at this beautiful complex was much needed after a 2 hour transfer from the airport. We were greeted by friendly and helpdul staff who really did want to help us in whatever we requested. Ahmet from MTS was available to make sure we certainly had a relaxing stay and helped us out with good discounts when using the hotel Spa. The decor and views from the room were outstanding and we couldn't have asked for more from the variety of food that was on offer. Kader from guest relations was also available day or night to ... ensure we got the most out of our stay and had the right balance of interaction with guests, not overbearing like some other hotel's we have used. Although we did not use one of the several pools on offer (a bit too cold in March) they were all avaiable and were immaculate. As an AI guest we had the opportunity to dine at one of the roof top restaurants which capped off a weeks relaxation with fine dining, a really good week left us looking at booking return date in the future. read hotel review
Family holiday
- Recommended hotel
- Families
- Beach holiday
- March 2015
Very nice hotel, great verity of foods, lots of Entertainment for kids, great view and trips trips from Ahmet P. MTS, thanks to manager Miss Kader who let us stay longer than the check out time. Will recomend for a family holiday. read hotel review
Goldcity Hotel Mar 2015
- Recommended hotel
- Singles
- Beach holiday
- March 2015
This is my first visit to the Goldcity Hotel and i have only been here for a few days and i am very impressed with the service very friendly staff from the animation team to the sap amd wellness centre. The gym great. The weather is not what i expected but theres nothing anyone can do about this as it is out of season! read hotel review
Great holiday
- Recommended hotel
- Couples
- Beach holiday
- March 2015
Overall a great visit came during off season so some facitilities were mot available however still enough for an enjoyable time. The all inclusive food was repetitive and room service was very fast and tasted good. We were helped by the staff when needed and offered excursions by a cery good guy called ahmet he along with beberto made our holiday top. Would definitely visit again read hotel review
Holiday at Goldcity
- Recommended hotel
- Couples
- Beach holiday
- February 2015
Very nice place, very good food, very good service, very good price. I would like a place to sit down with my laptop and work for a couple of hours every day. ... read hotel review
Information about Hotel Goldcity Tourism Complex
Hotel Information
Information from HolidayCheck
Information from HolidayCheck *
Hotel Beach
- What the hotel offersGoldcity Tourism Complex The 5-star Goldcity Tourism Complex overlooks the Mediterranean Sea and is favored by families, groups of friends and solo travelers visiting Alanya - Kargicak on vacation. The Goldcity Tourism Complex has 242 rooms. From the hotel guests can see the Mediterranean Sea and the Taurus Mountains. The award winning spa offers massages and a hammam. There are several restaurants serving Italian, Mediterranean, Turkish and Mexican cuisine. Wireless internet access is available in the public areas of the hotel free of charge. Check-in is available from 14:00 whilst check-out is until 12:00 noon.
- LocationThe Goldcity Tourism Complex is situated on a hill overlooking the Mediterranean Sea. From the hotel guests can see the Taurus Mountains. The hotel is a 25 minute drive from Alanya and is about 2.5 kilometers away from the Mediterranean beaches where guests can sunbathe and go swimming. Watersport activities such as water skiing and snorkeling can be enjoyed by guests near to the hotel. The hotel offers a shuttle bus service. Accomodation Each room has an en suite bathroom and is fully air-conditioned. The rooms also have a seating area and a satellite television whilst some rooms have views over the Mediterranean Sea or the Taurus Mountains.
- Food and DrinkThe hotel has a wide range of dining options covering all tastes. These include Mexican, Turkish, Mediterranean and Italian cuisine. The restaurants all have live music and special menus. There are 6 different bars where guests can enjoy alcoholic and non-alcoholic drinks including cocktails. Visitors to the hotel can also relax by the pool with a drink.
- LeisureThe hotel has an indoor and outdoor pool as well as an award winning spa that offers a gym, sauna, a hammam, hot tub and massages. The Goldcity Tourism Complex also has a tennis court and a games room with billiards and table tennis. Other leisure activities include bowling, darts and mini-golf. Children can make use of the playground. In Alanya - Kargicak guests can go cycling, horse riding and snorkeling. Other services The hotel offers a dry cleaning and ironing service. There is a tour desk, a currency exchange service and a cash machine in the hotel.
* HolidayCheck does not assume liability for the accuracy and completeness of the content displayed above. Please bear in mind that the services included in an offer may differ at the time of booking from the description presented here. Solely those services available at the time of booking as shown on the booking form will be offered.
Hotel description
Hotel description
- Address::Kargicak - Alanya 07435 Kargicak
Email: firstname.lastname@example.com
Phone: 0090 242 510 02 00
Facebook: www.facebook.com/goldcityhotel
Website: http://www.goldcity.com.tr
- What the hotel offersSet upon a hilltop, Goldcity Hotel & Spa has uninterrupted views of Alanya's mountains and coastline. It features a 3,500 m² spa, outdoor pools, a private beach, and a sport complex. Alanya beckons to be discovered!
- Location115 km to Antalya Airport 30 km to Gazipaşa Airport 17 km to Alanya City Center
- AccommodationThe rooms at Goldcity Hotel are decorated with elegant furniture and warm colours. All rooms have a flat-screen TV with satellite channels, a bathroom with a whirlpool bath, and a private balcony.
- Food and DrinkGoldcity offers you a variety of restaurant and bar choices with different cuisines.
- LeisureThe Goldcity Sport Complex offers various sports facilities, such as 2 grass football fields, 6 tennis courts with lighting system, volleyball and basketball playing ground, badminton, running track, fishing, artificial lakes and sport bar. The hotel also offers cinema, bowling alley, night club, daily entertainment programme, mini club, mini golf, land chess, game room, giant trampoline, beach volley and water sports. Goldcity offers variety of pools; 1200 sqm pool with an aquapark, olimpic length swimming pool, kid's pool, relax pool and indoor pool. Goldcity has also 15,000 sqm private beach.
- Other facilities and servicesGuests can choose from a wide variety of health and beauty treatments at the Aqua Verde Spa, which also features a dry sauna, a steam room, a hammam, and a fitness centre. Free private parking is available on site.
Hotel amenities
- Garden
- Meeting/Banquet Facilities
- Apartment
- Shops in the hotel
- Hairdressers/Beauty salon
- Playground
- Hotel safe
- Lift
- Central heating
- Air conditioning
- Free parking
- Car park
Room amenities
- Kitchenette
- Single bed
- Satellite TV
- Air conditioning
- Minibar
- Safe
- Coffee and tea
- Balcony
- Bathroom
- Electronic room keys
- Wake up call/Alarm clock
- Complementary bottle of water
- Toiletries
- Breakfast
- Restaurant
- Vegetarian dishes
- Menu
- Hotel bar
- Continental breakfast
- Packed lunches
A choice of sports
- Fitness centre
- Outdoor pool
- Aqua park
- Tennis court
- Bicycle hire
Spa offers
- Spa centre
- Massages
- Special massages
- Sauna
- Steam room/ turkish bath
- Whirlpool
Other hotel information
- 24 hour reception
- Evening programs and entertainment
- Number of stars/domestic category: 5 Stars
- Check in from: 14:00
- Babysitting/Child services
- Kids club (6-9 years)
- Check out no later than: 12:00
- English
- Family
- Airport transfer
- Shuttle bus transfer from the airport: charged
- City
- Cash payment
- German
- 24 hour security
- Car hire
- Information desk
- Currency exchange
- All public and private areas are no smoking zones
- Wifi
- Free Wifi access included
- Newspaper
- Dry cleaning
Nearby - Hotels and Things to do
- Hotels
- Things to do & See


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New York based perfume house Le Labo and L.A. based T-shirt label Hiro Clark have joined forces to present a scented T-shirt collaboration romantically named Smells Like You. The... more >
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Blemish-free: 10 best skincare products
Ease your teens into a skincare regime with these winning products for young and sensitive faces
Wednesday 26 March 2014
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Prisoners (US - DVD R1 | BD RA)
WB has news for the Hugh Jackman, Jake Gyllenhaal kidnapping thriller...
Starring: Hugh Jackman
Released: 17th December 2013
Further Details:
Warner Bros. has a release date and cover art for their Blu-ray Combo Pack (Blu-ray/DVD/UltraViolet) and DVD releases of Denis Villeneuve's tense drama,
Prisoners. There are no DVD specs for the time being, but WB did include the back cover art for the Blu-ray Combo Pack will feature:
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Quote: How far would you go to protect your child? Keller Dover (Hugh Jackman) is facing every parent's worst nightmare. His six-year-old daughter, Anna, is missing, together with her young friend, Joy, and as minutes turn to hours, panic sets in. The only lead is a dilapidated RV that had earlier been parked on their street. Heading the investigation, Detective Loki (Jake Gyllenhaal) arrests its driver, Alex Jones (Paul Dano), but a lack of evidence forces the only suspect's release. Knowing his child's life is at stake, the frantic Dover decides he has no choice but to take matters into his own hands. But just how far will this desperate father go to protect his family?
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Tel: (617)445-0564
School Overview
|Religious Affiliation
|Grades Offered
|Grades Kindergarten - 3
Student Body
|Total Students
|57 students
|Student Body Type
|% Students of Color
State avg.: 25%
|Students by Grade
Academics and Faculty
|Total Classroom Teachers
|4 teachers
|Student : Teacher Ratio
National avg.: 13:1
Finances and Admission
Nearby Schools:
All Schools | High Schools | Elementary Schools | Preschools | Public Schools
Show me:
- School Location Miles Students Grades
DorchesterCrispus Attucks Childrens Center
105 Crawford St
Dorchester, MA 02121
(617)445-1420Daycare / Preschool (Non-sectarian)0.6 mi | 80 students | Gr. K
RoxburySt Patrick School
131 Mount Pleasant Ave
Roxbury, MA 02119
(617)427-3881(Roman Catholic)0.6 mi | 204 students | Gr. PK-8
RoxburyPaige Academy
28 Highland Avenue
Roxbury, MA 02119
(617)442-5419Special Program Emphasis0.7 mi | 106 students | Gr. PK-K
Jamaica PlainNativity Preparatory School
39 Lamartine St
Jamaica Plain, MA 02130
(857)728-00310.9 mi | 58 students | Gr. 4-8
All-boys (Roman Catholic)
Jamaica PlainCopper Beech Montessori School
179 Amory St
Jamaica Plain, MA 02130
(617)524-3237Montessori School (Non-sectarian)0.9 mi | 67 students | Gr. NS-PK
School Reviews
Area Statistics
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Updated March 26, 2015
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IDG Strategy Guide: Identity and Access Management Guide
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La Junta 81050, CO, United States2 days ago on ebay.com
For sale Auction
Nice Antique metal quick meal ranges grate shaker / lifter. Reads " quick meal ranges" " 840". Condition is great, no cracks or chips.
wood stove colorado
Delaware, NY, United States6 days ago on craigslist.org
For sale
Split Firewood. Sold by price ranges: Number 1 Range: (125/Cord) An assortment of what is split. (No Pine or any kind of Evergreen) Number 2 Range: (200/Cord) An assortment of just Maple, Oak, Birch and Walnut. Number 3 Range: (250/Cord) Your Choice of Wood. Currently ready to deliver Maple, Poplar and Walnut. Delivery available. Price depends ...
firewood delivery new
Rutland, VT, United States2 weeks ago on ebay.com
For sale Auction
This trivet measures 7 1/4 inches across and dates from circa 1900-piece is very unusual in that it has black and tan patina on the cast iron-excellent condition with no chips or cracks-paypal-$8.00 priority shipping in the usa-no international shipping
vermont stove for sale
Downs 67437, KS, United States2 weeks ago on ebay.com
For sale $17
Rmsdivision Store - ask about my others .combined shipping rates available I sell everything as found, described and pictured.----will ship overseas--email for shipping cost-- email any questions about shipping or payment. ----shipping weight # -to save you money I ship mostly parcel post unless stated different in listing, which takes longer for delivery. Priority delivery is available too. -Shipping ...
wood burning stove ks
Grand Junction CO, United States3 weeks ago on ebay.com
For sale $24
"Bucks Stoves and Ranges" Wood Stove Burner Cover Plate #2 on reverse...Cast Iron, Nickle Plated 6 1/4" in diameter, has hanger attached to be used for display...sits 1 1/2" off the wall We have an eclectic store and pride ourselves in presenting a variety of interesting and unique old and new items to all who ...
antique stoves,
antique wood burning stoves
Washington 20081, DC, United States1 month ago on ebay.com
For sale $39
Antique Foster Stoves & Ranges Catalog No 37 Wood Coal Gas. 94 pages. Some wear, staining, loose binding - mostly back cover loose upper half. Pencil writing on a few pages. Neat as shown in pic. Email with any questions. I combine shipping so check out my store for other items. :)
antique gas stove
Spring Hill 37174, TN, United States5 hours ago on ebay.com
For sale Auction
Up for Auction Princess Quality Crown Pin, Princess Steel Ranges Nashville, Tennessee Pin seems to be missing 1 1/2 inches length Please refer to photos provided. The item that is pictured is the item you will receive. Please refer to the pictures provided for more details. Please feel free to email us with any questions or concerns you ...
Villanova 19085, PA, United States5 hours ago on ebay.com
For sale $25
...s measures 8 3/8" x 14", and is from Morris & Haines Cooking And Heating Ranges, 121 &123 N. Sixth St, above Arch St., Philadelphia, pa. The letter has great vintage graphics at the top of a range. It has it's original folds as was mailed, and two small tears at the top. This would make a great looking ...
Barberton 44203, OH, United States5 hours ago on ebay.com
For sale $74
Runs a little scan for frequencies used in hidden bugs/cameras, beeps if you have it next to anything sending out a signal. Looks like a key fob for a car alarm but bigger. Says ghost finder so you can say your just searching for a ghost.
Moyock 27958, NC, United States6 hours ago on ebay.com
For sale Auction
...premium plastic all pdga approved discs. These discs are in excellent playing condition. All distance ranges included details: puttergateway sss wizard super stupid soft putter - 175 gramscondition - 10/10 newcolor - tannotes - Amazing, putter, a must have! Mid-rangedga sp line aftershock - 174.9 gramscolor - red , metal flakescondition - 8.5/10,excellent like new, faded inknotes - This is an amazing disc ...
Springfield MA, United States1 day ago on ebay.com
For sale $3
Up for bidding is the lot of parts for the Power Rangers Time Force Megazord Mode Red Robot. The winning bidder will receive the actual item shown. The item is used and in played with condition. Please see pics for details. Items are the left arm of the robot (right side if looking at robot), Shield, and its sword. ...
Hampden 01036, MA, United States1 day ago on ebay.com
For sale Auction
A nice 32mm 1946 Monarch Ranges 50th Anniversary Medal gilt coming off in places Powered by sixbit's ecommerce Solution
Seattle 98115, WA, United States2 days ago on spreadmyad.com
For sale $375
Two brand-new in the box gas ranges available. $375 each. For features and specifications check out the following links: http://www.frigidaire.com/Kitchen-Appliances/Ranges/Gas-Range/ffgf3053ls/ Purchased by a construction business that is closing, so these are overstocked items. Reply by Craigslist email relay, or call Mike at show contact info
Hazel Park 48030, MI, United States2 days ago on ebay.com
For sale $1
Recipes, Care, Use Westinghouse Speed-Electric Ranges 1954, soft cover with 80 pages Vintage instruction and recipe booklet for the Westinghouse Speed Electric Range published in 1954. Contents include; Which if your range? How to use surface units How to use miracle sealed ovens How to use automatic timers How to care for your range Recipes and cooking tips ...
Horicon 53032, WI, United States3 days ago on ebay.com
For sale Auction
2 inch picatinny rail that attach to Amega Ranges scout rails. Allows you to mount lights, lasers, etc.
Follow this search and create an email alert for Ranges
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Manufacturing Area
Category: Packing/Packaging Machines
Paper corner protector Manufacturers Advantages: Protect their products from the available beams together so that the overall package more solid. Fixed on pallets of goods Can serve to protect product ...
Category: Nail Care Products (Polish, Lotion, etc.)
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NAS-K330 is a multi-function network downloader. The chipset is Star 8132. It owns one Lan port and two USB ports for connecting mobile hard drive, MP3, MP4, USB drive etc. It supports Printer USB ...Keyword : network attached storage | NAS storage
Category: Home Automation Controllers/Consoles
Smart Home kit(Support 433MHz/Zigbee /Z-Wave)/Security Protection/Home Entertainment/Energy Saving/Remote Control 3 in 1 smart controller ,android TV box, wireless router. 1.Smart home control:smart ...


text: © The Hangover Car - Credit: Mercedes-Benz Back to Top ▲ The Hangover Car 0 Shares Share Tweet The Hangover Car 2/10 The Hangover Car Thankfully, the interior was fully restored after Mike Tyson's tiger had his way. Next Slide Comments Show Comments More Like This Car Tips Pushing An Audi To The Limits On Sweden's Frozen Lakes In A Drag Race For The Ages Read More Car Reviews We Drove The 2015 Mercedes S63 AMG Coupe And It Was Awesome Read More Car Reviews Our Take On The New Jeep Wrangler Black Hawk (Hint: It's A Beast) Read More Car Tips Volvo Is Saying That No One Will Die In Its Cars By 2020 Read More Car Tips These 5 Sporty Cars Are Bringing The Wagon Back From The Dead Read More Car Reviews Does BMW's First Ever M Performance Convertible Live Up To The Hype? Read More Promoted Around the Web Special Features Special Feature From Dating To Fitness, Over 75 Ways Men Are Winning 2015 Read More AskMen Exclusive Want To Win $1,000 To Stock Your Home Bar? Read More Special Feature 50 Ways To Look Better Than You Do Now. Smooth Up, Man. Read More


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Blick devotional picture Anmut Apollo marble ivory mourning sandstone alabaster horse Mary cross death Rome Christus angel limewood Germany bust strength Ulm Wiederholung Crucifixion Johannes Faltenwurf book drama gold Haare antiquity
Ariadne on the Panther
Johann Heinrich von Dannecker received his initial training as a sculptor in his native town of Stuttgart. In 1776 he committed himself to ducal service for life. Appointed court sculptor to the Duke of Württemberg in 1780, he continued his…
Copyright © 2015 Liebieghaus. All rights reserved.


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1995 Caterpillar 311 Excavator
Excellent Condition, 38 " Bucket, Runs well!, Equipment will speak for itself. The only reason selling is because he needs to retire. There are no issues with it! Priced to sell!
- See more at: http://www.heavyequipmentregistry.com/heavy-equipment/13114.htm#sthash.2WIZkiHx.dpuf
Feb 11 -
Flashing Arrow Sign with 1-Cylinder Diesel by Wanco
1995 Minnco Sign trailer with wanco Generator in good working order Flash's Left, Right & Both way,
Mar 28 -
Minneapolis-St. Paul :
1946 John Deer Model A with Saw arbor Mounted on front, Start & Run Great, Been in storage, Will Sell or Trade for what have you (vintage Antique Tractor)
**SPECIAL for Local Pick Up**
Weld on Rock Stinger Kit for 4X4 front bumpers.
This Stinger is Engineered to provide ease of installation and extreme durability.
Like all of our products, you can count on a CAD Designed part that incorporates our unique Tab & Slot ...
Mar 27 -
Minneapolis-St. Paul :
(815) 828-4005 ext.182
Low mile SilverwingThis Silverwing only has 1216 miles on it ! Has minor scratches on left side....
Copy & Paste the above link for full vehicle details
Mar 25 -
Rockford :
Looking for a useable auger style header for an 8830 International haybine
Mar 25 -
Minneapolis-St. Paul :
Used E-Z Go Textron Total Charge II $140 cash or credit ID# 112172
Does have some small rust areas on top of charger but still works great
If you are interested in buying, no need to meet. It's available for pick up at Deals Depot new bigger & better location
3320 ...
Mar 24 -
Minneapolis-St. Paul :
Brooklyn Park
Get 'em up and outdoors with this mini 410BXR 110cc quad. The added rear utility rack is perfect for light yard work or chores around the house. This quad is ready to take on the trails. Push-button electric ignition makes getting going fast and easy. Front and rear ...
Mar 23 -
Sioux Falls :
Sioux Falls
Specialty Auto Tool Rentals.com
We RENT Engine timing set and Suspension tools required to work on European and domestic cars
Place an order on line at Specialty Auto Tool Rentals.com
Mar 23 -
Minneapolis-St. Paul :
1978 Club Car 48vdc Electric Golf Cart 4 Sale in mint condition
All new Battery's.
New Battery Charger.
New tires.
New Paint.
New seat upholstery.
New brushes in rebuilt Motor.
New Wiring.
Comes with Golf bag and clubs.
Comes with front mounted winch.
Has turn lights.
Has ...
Mar 21 -
Chicago :
Spring Grove
(815) 828-4005 ext.397
MAJESTY W/STEREO !This bike is perfect ! It has just been serviced and has new tires ! NO body damage anywhere ! Has radio w/mp3 plug !...
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Mar 20 -
Rockford :
1989 Buick Century Service Manual
Very Good Condition: No torn or missing pages
General Information
Heating and Air Conditioning
Body, Frame and Bumpers
Steering, Suspensions, Wheels and Tires
Drive Axle and Propeller Shaft
Chassis and Body ...
Mar 17 -
Chicago :
1998 Hydramaxx trencher. 7ft wheel. auto tilt. auto dig. Complete gps system. Cab with heat. New #1 drives and new batteries. Excellent condition and 100% field ready.
- See more at: http://www.heavyequipmentregistry.com/heavy-equipment/14138.htm#sthash.fdHUh6Fu.dpuf
Mar 17 -
Des Moines :
Van Meter
Here's a Once and Once Only REDUCED price... $2,200 FIRM -- If you don't want it this way it will be $3,400 when summer hits -- SEE DETAILS BELOW WHY -- I Need It Out Of My Way Now
I have an excellent condition EZ-GO with lift, Aluminum wheels and Gripper tires, Chrome ...
Mar 16 -
Rochester (MN) :
(605) 385-0293 ext.562
Copy & Paste the above link for full vehicle details
Mar 16 -
Sioux Falls :
Sioux Falls
(920) 663-6083 ext.97
2015 Triton 26 foot tandem axle pontoon trailer in great condition. Its 43" wide and rated for up to 3,480#. Call today!...
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Mar 13 -
Madison :
Used Pair of Heavy Duty, Aluminum 10Ft 2500# Big Boy II Folding Ramps. Used once and stored since. These ramps can be clamped together to make one 38 inch wide ramp as shown in photos. Clamps not included.
Only the ramps are for sale. Nothing else in pictures is included.
Mar 12 -
Dubuque :
$2350 cash only firm
Buyer pays shipping
**leave me message on my office voicemail
**Item sold as is without return or guarantee..
never used. new in box
pro charger 600 b for small block chevy. flows approximately upto about 1100 cfm and 14 lbs boost, this kit runs ...
Mar 12 -
Chicago :
(605) 385-0293 ext.997
Copy & Paste the above link for full vehicle details
Mar 12 -
Sioux Falls :
Sioux Falls
(920) 663-6083 ext.94
2600# Shorestation vertical boat lift. 108" wide, as shown....
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Mar 10 -
Madison :
(920) 663-6083 ext.93
NEW Shorestation canpy vinyl. 22' x 108" beigh vinyl....
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(920) 663-6083 ext.92
Boat Slips available for 2015 summer season. Quiet, secluded location on Lake Sinissippi. Season runs May 15 thru October 15, 2015. Availability is limited....
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I have a 880 Owatanna end loader. Perkins diesel. Runs good but cold blooded. Tires fair. Would make a good feed loader. Bought a skid steer to replace
- See more at: http://www.heavyequipmentregistry.com/heavy-equipment/14054.htm#sthash.AF44hVeX.dpuf
Mar 10 -
Cedar Rapids :
Central City
2014 "Dominator" Models
(Red Pictured, Black also Available and in Stock at the moment)
AWESOME Features included at NO EXTRA COST! (vs. Other Brand UTV's)
Hard 1/2 Doors
Front AND Rear 3500# Winches
Hard Roof
FULL Wind Shield
12" Aluminum Wheels
LED Lights
Mar 9 -
Minneapolis-St. Paul :


text:
Pilates and More: The Body-Mind Connection
At Pilates and More we are all about women. Women of all different shapes and sizes! Whether you are a top athlete or a beginner, young or old, thin and not-so-thin, we are here to keep you healthy, happy and fit!
At Pilates and More we provide you with an alternative experience in fitness. By combining cutting edge technology, state of the art equipment, and innovative techniques, we provide you with smarter solutions to energize and motivate your body & mind... for life!
|We are inviting you to join our INTERNATIONAL WOMEN'S DAY CHALLENGE this coming March 16 Mahboula Branch (Gulf Road, Mareena Towers), March 17 in Salmiya Club (Baghdad ST.), March 18 in Hawally Club (E-Mall, front of Sultan Center Hawally), from 6-8pm for FREE. Come along with your family and friends. Beat your stress! Loss weight! Tone your muscles! Have fun! Wit our Step Aerobics and Flowin Class. See you in Pilates and More Women's Health Club. For inquiries please call our Membership Advisors Salmiya Branch/Mahboula Branch/Hawally Branch 22207123
Follow us to get the latest updates in fitness.
Pilates and More (Salmiya and Mahboula) @PNM_Q8
Easyfit (Hawally) easyfitgym
Thursday's Classes
Morning Classes
PILATES REFORMER 1* / Starts 10am
PILATES-YOGA FUSION / Starts 10am
DRUMBEATS / Starts 11am
IRON YOGA / Starts 12pm
Afternoon Classes
X-TRAIN BASE (MAT) / Starts 1pm
YOGA FOR WEIGHT-LOSS / Starts 2pm
STEP AEROBICS 1 / Starts 3pm
Firm Body Awesome Abs Circuit / Starts 4pm
Evening Classes
AEROBICS HIGH LOW 2 / Starts 5pm
GYMSTICK MUSCLE / Starts 6pm
JUMPING / Starts 7pm
PILATES REFORMER 1* / Starts 7pm
FLOWIN / Starts 8pm


text:
The Easter Bunny's Guide to Candy Personalities
Today on The Daily Meal
Recipe of the day
Most Recent
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Easter is just days away, which means there's not much time left for the Easter Bunny to prep candy-filled eggs and strategize for egg-hunts. And with so many children anxiously awaiting their holiday sugar-high, it's important to make sure that each kid is getting the right candy to fit their unique virtues and personality. That's when this guide comes in handy —so listen up Easter Bunny, The Daily Meal is here for you.
Click here for The Easter Bunny's Guide to Candy Personalities Slideshow
When it comes to a treat as mood-altering and addictive as candy, it's imperative to know what kind of personality you're dealing with before you offer up the goods. You don't want to make the classic mistake of filling eggs with sour jelly beans for a boy who's shy and anxious —that's a recipe for disaster. However, an adventurous girl who loves to explore her surroundings is likely to jump for joy upon receiving an egg-full of pucker-inducing treats.
That said, determining the best candy for a child's personality is not an exact science; it takes a keen sense of intuition and a tendency toward whimsical thinking. However, there are a few basic tenants that are sure to make the job easier — pair sweet, innocent young ones with mild-flavored, sugar-coated confections, like gum drops or peach rings; and pair thrill-seeking tykes with something a bit spicy or sour, like cinnamon- or lemon-flavored candies.
Click through the slideshow for the inside scoop on other sure-bet pairings.
Be a Part of the Conversation
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text:
Written on: 09/05/2013
The booking phase was very good and prompt. I was disappointed that I could not talk to an agent who had a reasonable knowledge of Venice and the use of waterbuses and land buses. The hotel was very good and conveniently sited. The city is very interesting and busy. We plan to return in a few years time
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text:
Econo Lodge City Center
1889 SW 4th Avenue
Portland, OR 97201
Phone: (503) 226-7646
Fax: (503) 459-4000
Places Events
Multnomah County Central Library
View More >
801 Southwest 10th Avenue
Portland, OR 97205
Pioneer Courthouse Square Fountain
View More >
715 Southwest Morrison Street
Portland, OR 97205
Northwestern National Bank Building
View More >
621 SouthWest Morrison Street
Portland, OR 97205
ODS Tower
View More >
601 Southwest Second Avenue
Portland, OR 97204
Waldo Block
View More >
215 SW Washington Street
Portland, OR 97204
Eastbank Esplanade
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South East Water Avenue & Hawthorne Boulevard
Portland, OR 97214
Dark Star OrchestraCrystal BallroomApril 02, 2015 8:00 PM
1332 West Burnside Street
Portland, OR 97209
Have you ever loved something so much that you couldn't let go? A dying pet that you've come to love, a significant other that's starting to wreak havoc on your soul, or, in this case, a band that ran its dutiful course decades ago. The Grateful Dead was a wonderful sonic phenomenon that helped breathe life into the jam band genre, but after decades spent on the road the band became somewhat of a cliche of itself and after lead guitarist Jerry Garcia died in 1995, the group was done and it disbanded. But a few musicians decided to recreate the Dead's music on a regular basis. This band is called Dark Star Orchestra and it's basically a Grateful Dead cover band that performs tried and true tunes that helped make the Dead a jam band establishment.View More >
Soul'd Out & Double Tee Present: MONOPHONICS: Sound of Sinning Tour with Gene Washington & The Ironsides + DJ AquamanStar Theater April 02, 2015 9:00 PM
13 Northwest 6th Avenue
Portland, OR 97209
Ages 21+View More >
DOOKIE JAM featuring TONY OZIER & THE DOO DOO FUNK ALLSTARSDante'sApril 02, 2015 9:00 PM
350 West Burnside Street
Portland, OR 97204
All are welcome, bring on the funk.Tony Ozier and his ambassadors of funk are here to enlighten your mind and electrify your soul! Be ready for some otherworldly vibrations from Tony Ozier, the Doo Doo Funk All Stars, and a whole room full of ridiculously talented folks from the community.The funk is out there, and EVERY FIRST THURSDAYAges 21+View More >
The People's Republic Of PortlandEllyn Bye StudioApril 02, 2015 7:30 PM
128 NorthWest Eleventh Avenue
Portland, OR 97209
Star of HBO's "Looking" and former "Daily Show" correspondent Lauren Weedman is back with her hit solo show "The People's Republic of Portland". The Rose City has been getting a lot of attention lately -- for leading the way in sustainability, for its food scene and for being, well, weird. Whether she's poking fun at the city's kombucha obsession or its love of ecstatic dance, Weedman brings her unique perspective to all things Portland. Weedman previously performed "The People's Republic of Portland" to sold-out crowds at Portland Center Stage, following her previous hit, "BUST". Now she's reprising this hilarious show at the Gerding Theater at the Armory.View More >
Ladies RockDixie TavernApril 02, 2015
32 Northwest 3rd Avenue
Portland, OR 97209
Every Thursday night ladies get in free.View More >
Machouse Entertainment Presents: Empire Showcase ChicagoThe Someday LoungeApril 02, 2015 8:00 PM
View More >
125 Northwest 5th Avenue
Portland, OR 97209
Earn valuable points toward free nights.


text:
All News
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More Connacht News
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Connacht News
Connacht Make Four Changes For Interpro Tie
27 Mar, 11:45
Connacht head coach Pat Lam has announced the team to face Munster in Saturday's GUINNESS PRO12 derby match at Thomond Park (kick-off 5.15pm).
Flanker Fox-Matamua Announced As Connacht's Latest Signing
25 Mar, 08:05
Connacht Rugby and the IRFU have announced the recruitment of back rower Nepia Fox-Matamua who will join the province in the summer.
Leinster And Connacht Add Players For European Games
24 Mar, 11:59
Four former winners of European club rugby's elite tournament are included in the list of additional players for the knockout stages of the European Champions Cup and Challenge Cup.
Marmion And McKeon Available For Connacht's Match Against Munster
23 Mar, 17:18
Ahead of Connacht's crunch GUINNESS PRO12 round 18 encounter with provincial rivals Munster at Thomond Park next Saturday (kick-off 5.15pm), the westerners' medical team provided the following update.
Irish Rugby TV: Robbie Henshaw Savours Six Nations Success
21 Mar, 22:57
Speaking to Irish Rugby TV after the trophy presentation, RBS 6 Nations winner Robbie Henshaw said: "Words can't describe it...just the whole atmosphere, everyone in the stadium, just the way it ended out. It was pretty intense, the heart was rushing."
Carolan Aiming To Finish U-20 Six Nations 'On A Positive Note'
20 Mar, 08:11
Ireland Under-20 head coach Nigel Carolan insists there is no lack of motivation as his side hope to finish the U-20 RBS Nations on a high by beating Scotland in Galashiels tonight.
Irish Rugby TV: Robbie Henshaw On Sharpening Up For Scotland
17 Mar, 16:20
Previewing this week's trip to Murrayfield, Ireland's Robbie Henshaw said: "Scotland have a really nice back-line, we're going to have to be sharp on our feet and defend really well. We'll have to match them physically and bring some line speed."
Henshaw Braced For Tough Encounter With Wales
12 Mar, 13:23
This very week back in 2012 Robbie Henshaw was preparing to captain Marist College to victory in the Connacht Schools Senior Cup. Just three short years later, the Athlone youngster is now central to Ireland's plans of retaining their RBS 6 Nations crown.
McFarland To Leave Connacht After 15 Years Out West
11 Mar, 15:21
Connacht assistant coach Dan McFarland will leave his role with the province at the end of the current season to explore another opportunity abroad.
Late Heartbreak For Connacht In Cardiff
6 Mar, 21:33
This result could come back to haunt Connacht at the end of the season as they watched Rhys Patchell convert Joaquin Tuculet's injury-time try to give the Cardiff Blues a dramatic 18-17 home win.
Lacey And Clancy To Referee Champions Cup Quarter-Finals
6 Mar, 14:23
Two of the four upcoming European Champions Cup quarter-finals will be refereed by Irish referees, John Lacey and George Clancy.
GUINNESS PRO12 Preview: Cardiff Blues v Connacht
6 Mar, 09:09
Connacht have a quick five-day turnaround to negotiate as they look to maintain their winning form on a tricky trip to ninth-placed Cardiff Blues.
Poolman Extends His Connacht Stay
5 Mar, 16:15
Connacht Rugby and the IRFU are pleased to announce that 25-year-old back Danie Poolman has signed a new deal that will keep him at the Sportsground until at least the summer of 2017.
Three Changes For Connacht's Cardiff Trip
5 Mar, 12:39
Connacht head coach Pat Lam has made three personnel changes to his team to face Cardiff Blues at BT Sport Cardiff Arms Park on Friday night (kick-off 7.35pm).
Knee Injury Sidelines Adeolokun For Three Weeks
3 Mar, 14:11
Ahead of Connacht's GUINNESS PRO12 round 17 game away to Cardiff Blues on Friday night, the province's medical team provided an update on injured players.
Connacht Take Treviso Apart In Seven-Try Win
1 Mar, 19:39
Connacht gave their bid to finish in the top half of the GUINNESS PRO12 a big lift with a bonus point win over Benetton Treviso this evening.
Out-half and man-of-the-match Craig Ronaldson leads an attack for Connacht during the first half of their home clash with Treviso
GUINNESS PRO12 Preview: Connacht v Benetton Treviso
27 Feb, 21:49
Connacht's battle to finish in the top half of the GUINNESS PRO12 continues on Sunday evening, as they look to win for the first time in three outings at the Sportsground.
O'Halloran And Adeolokun To Start For Connacht
27 Feb, 12:15
Connacht head coach Pat Lam has made two personnel changes to the team that will host Benetton Treviso at the Sportsground on Sunday (kick-off 5.15pm).
Boyle Confirmed As Connacht's Head Of Operations
25 Feb, 11:59
Connacht Rugby have announced that CEO of Mountaineering Ireland, Karl Boyle, has been appointed to the role of Head of Operations with the province.
Connacht Await Update On Leader's Knee Injury
24 Feb, 15:31
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List all words that contain jealousy
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Kathleen K. (Rinn) Lampinski, age 46, of Almond Way, Coventry, died unexpectedly at home on August 8, 2013. She was the wife of Gregory J. Lampinski. Born in Warwick, the daughter of Bernard R. … more
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Page 1
Chronic atomoxetine treatment during adolescence decreases
impulsive choice, but not impulsive action, in adult rats and alters
markers of synaptic plasticity in the orbitofrontal cortex
HaoSheng Sun & Paul J. Cocker & Fiona D. Zeeb &
Catharine A. Winstanley
Received: 24 March 2011 /Accepted: 16 July 2011
# Springer-V erlag 2011
Rationale Impulsivity is a key symptom of attention-deficit
hyperactivity disorder (ADHD). The use of the norepineph-
rine reuptake inhibitor, atomoxetine, to treat ADHD
suggests that the activity of the norepinephrine transporter
(NET) may be important in regulating impulsive behavior.
Many ADHD patients receive chronic drug treatment
during adolescence, a time when frontal brain regions
important for impulse control are undergoing extensive
Objectives The current study aimed to determine the effects
of chronic atomoxetine treatment during adolescence in rats
on two distinctforms of impulsivity inadulthood and whether
any behavioral changes were accompanied by alterations in
mRNA or protein levels within the frontal cortices.
Methods Rats received daily injections of saline or atom-
oxetine (1 mg/kg) during adolescence (postnatal days 40–
54). Two weeks later, animals were trained to perform either
the delay-discounting test or the five-choice serial reaction
time task (5CSRT).
Results Adolescent atomoxetine treatment caused a stable
decrease in selection of small immediate rewards over
larger delayed rewards (impulsive choice) in adulthood,
but did not affect premature responding (impulsive
action) in the 5CSRT. Chronic atomoxetine treatment
also altered the ability of acute atomoxetine to modulate
aspects of impulsivity, but did not change the response
to d-amphetamine. Ex vivo analysis of brain tissue
indicated that chronic atomoxetine decreased phosphory-
lation of CREB and ERK in the orbitofrontal cortex and
decreased mRNA for BDNF and cdk5.
Conclusions These data suggest that repeated administra-
tion of atomoxetine in adolescence can lead to stable
decreases in impulsive choice during adulthood, potentially
via modulating development of the orbitofrontal cortex.
Keywords ADHD.Impulsivity.Norepinephrine.Western
Impulsivity can be broadly conceptualized as acting or
making decisions without appropriate forethought, there-
by enhancing the potential for negative consequences.
High levels of such impulsive behaviors are a key
symptom of attention-deficit hyperactivity disorder
(ADHD), mania within bipolar disorder, and a diverse
collection of impulse control disorders not elsewhere
classified (pathological gambling, trichotillomania, inter-
mittent explosive disorder, kleptomania, and pyromania).
Impulsivity is also increasingly recognized as a risk
factor for the development of drug addiction (Moeller et
al. 2001b; V erdejo-Garcia et al. 2008; Winstanley et al.
H. Sun:P . J. Cocker:F. D. Zeeb:C. A. Winstanley (*)
Department of Psychology, University of British Columbia,
2136 West Mall,
V ancouver, BC V6T 1Z4, Canada
e-mail: firstname.lastname@example.com
Present Address:
H. Sun
Department of Neuroscience, Mount Sinai School of Medicine,
New York, NY , USA
DOI 10.1007/s00213-011-2419-9
Page 2
2010a). Understanding the neurobiological basis of im-
pulsivity could, therefore, provide valuable insights into
the nature of these diseases and suggest novel targets for
therapeutic treatments.
Impulsivity is a nonunitary construct (Evenden 1999;
Moeller et al. 2001a), and the dissociation between motor
impulsivity and more cognitive forms of impulse control
has been relatively well-established using comparable
behavioral methodology in both humans and laboratory
animals (Dalley et al. 2008; Winstanley et al. 2006). In rats,
impulsive action, or behavioral disinhibition, can be
measured using a variety of tasks including the five-
choice serial reaction time task (5CSRT), which is an
analog of the continuous performance test used for similar
purposes in humans (Carli et al. 1983). It is also possible to
determine impulsive choice across species using delay-
discounting paradigms which determine preference for
smaller-sooner vs larger-later rewards (Evenden and Ryan
1996; Hariri et al. 2006; Mazur 1987; Richards et al. 1997;
Wogar et al. 1993). Hence, insight into the neural and
neurochemical regulation of impulsivity can be obtained
from appropriate experiments in animals and can provide
the opportunity to test hypotheses that are difficult to
accomplish using human subjects.
The ability of psychostimulant drugs such as am-
phetamine and methylphenidate to improve impulse
control in ADHD has largely been attributed to the
drugs' actions on dopaminergic neurotransmission, and
the dopamine (DA) and serotonin (5-HT) systems have
been the focus of much of the work exploring the
pharmacological regulation of impulsivity (Pattij and
V anderschuren 2008). However, the noradrenergic system
could be just as critical in controlling levels of impulsive
behavior (Arnsten 2009; Biederman and Spencer 1999)
and is likewise affected by psychostimulant administration
(Berridge et al. 2006; Kuczenski and Segal 2001; 2002).
The norepinephrine (NE) reuptake inhibitor atomoxetine
became the first nonstimulant drug approved for the
treatment of ADHD (Simpson and Perry 2003; Spencer
and Biederman 2003; Spencer et al. 2002). In rodents,
atomoxetine (0–3 mg/kg) has been shown to increase NE
efflux in both the frontal cortex and striatum and also
increase DA efflux specifically within the prefrontal
cortex where DA clearance is largely regulated by the
norepinephrine transporter (NET) due to low levels of the
DA transporter (Bymaster et al. 2002; Koda et al. 2010;
Swanson et al. 2006). Unlike the acute administration of
psychostimulant drugs, which increase motor impulsivity
while generally decreasing impulsive choice in healthy
adult rats, acute atomoxetine has been found to decrease
impulsivity in tests of both impulsive choice and impul-
sive action (Bari et al. 2009; Barry et al. 2009; Blondeau
and Dellu-Hagedorn 2007; Chamberlain et al. 2006;
Navarra et al. 2008; Robinson et al. 2007; Tsutsui-
Kimura et al. 2009). Although psychostimulant drugs do
inhibit NET activity (Berridge et al. 2006; Kuczenski and
Segal 2001, 2002), such behavioral data indicate that
atomoxetine's effects can be partially dissociated from
those of the psychostimulants and that different tests of
impulsivity in normal animals can be used to evaluate its
therapeutic effects.
However, acute administration in adulthood does not
capture the manner in which these drugs are administered in
clinical populations. Although ADHD can manifest de
novo in adulthood, the majority of individuals with ADHD
are diagnosed during late childhood or adolescence and
treated chronically with pharmacotherapies throughout this
developmental stage. Areas of the brain, particularly within
the frontal cortex, are still undergoing extensive maturation
during this period (Casey et al. 2005). Furthermore, it has
been suggested that ADHD may arise due to retardation in
individuals' neurodevelopmental trajectories in that cortical
thinning fails to occur as rapidly (Shaw et al. 2007, 2011).
Given that different regions of the frontal cortex play an
important role in controlling both impulsive action and
impulsive choice, determining whether repeated pharmaco-
therapy administration during adolescence affects neuronal
function in this region may provide valuable insight into
their beneficial effects.
There are also concerns as to whether drug treatment
during this time could actually have negative, rather than
positive, effects on behavior in adulthood (Berman et al.
2009; V olkow and Insel 2003). Although repeated admin-
istration of methylphenidate during adolescence in rats has
been reported to decrease impulsive choice (Adriani et al.
2004; Leo et al. 2009), such treatment can also affect
numerous aspects of behavior in adulthood, decreasing
sensitivity to natural rewards such as sucrose or sex, while
increasing sensitivity to aversive stimuli (Bolanos et al.
2003). Whether such treatment enhances or diminishes
sensitivity to drug reward is unclear (e.g., Brandon et al.
2001, 2003; Burton et al. 2010; Carlezon et al. 2003).
Nevertheless, it would appear that methylphenidate admin-
istration during adolescence can have long-lasting effects
on dopaminergic neurotransmission (Brandon et al. 2003;
Moll et al. 2001) and gene expression within the nucleus
accumbens, a key site of action of addictive drugs
(Andersen et al. 2002; Brandon and Steiner 2003).
However, little is known regarding the consequences of
repeated administration of atomoxetine during adolescence.
The aims of the current experiments were therefore
twofold: (a) to determine whether chronic atomoxetine
treatment during adolescence would produce long-lasting
changes in impulsive behavior in adulthood and (b) to
identify a putative mechanism of action for any drug effects
observed through determining the behavioral response to
Page 3
psychostimulant and noradrenergic drug challenges and via
ex vivo analysis of gene and protein expression within the
frontal cortex and nucleus accumbens.
Materials and methods
Subjects and drug treatment during adolescence
Subjects were 32 male, Long Evans rats (Charles River
Laboratories, St. Constant, Canada), obtained at postnatal
day (PND) 21. Animals were pair-housed in a colony room
(21°C) under a reverse light cycle (lights off at 8 a.m.).
Water was available ad libitum. Starting at PND 40, half of
the subjects received once daily intraperitoneal (i.p.)
administration of vehicle (0.9% saline), while the other
half received i.p. administration of atomoxetine (1 mg/kg)
for 14 consecutive days. Given that the half-life of i.p.
atomoxetine in rats is estimated at ∼3 h (Mattiuz et al.
2003), the effects of repeated i.p. injections will not mimic
that of slow-release drug formulations. Nevertheless, such
dosing regimes have been used previously to investigate the
impact of chronic rather than acute administration of
atomoxetine and similar drugs in rodents (Koda et al.
2010; Sacchetti et al. 1999; Tanda et al. 1996), particularly
as oral dosing with atomoxetine is not ideal in rats due to
more efficient first-pass hepatic clearance, leading to low
bioavailability of the drug (Mattiuz et al. 2003). The dose
of 1 mg/kg was chosen based on previous reports showing
that this dose produced reliable effects on measures of
impulsivity and also increased both NE and DA in the
prefrontal cortex (e.g., Bymaster et al. 2002; Robinson et al.
2008). Subjects had free access to standard rat chow until a
week prior to behavioral training when they were restricted
to 14 g of food per day (exclusive of any reward obtained in
the operant chamber) to maintain approximately 85% free-
feeding weight. Behavioral testing and training took place
between 8 and 11 a.m. 5 days/week. Testing and housing
were in accordance with the Canadian Council of Animal
Care, and all experimental protocols were approved by the
Animal Care Committee of the University of British
Behavioral testing
Testing for both the 5CSRT and the delay-discounting
paradigm took place in eight five-hole operant chambers
(Med Associates, St. Albans, VT, USA) as described
previously (Zeeb et al. 2009). The boxes were controlled
by software written in Med PC (Med Associates, St.
Albans, VT, USA) by CAW running on an IBM compatible
computer. Prior to training, all animals were first habituated
to the operant chambers over two daily 30-min sessions
during which sucrose pellets were placed in the response
holes and food magazine.
The 5CSRT paradigm
Details of the training and testing procedures have been
provided previously (Carli et al. 1983; Winstanley et al.
2007, 2010b). In short, animals were trained to make nose-
poke responses into the response apertures upon brief
illumination (0.5 s) of the light located therein. The
stimulus light could appear in any of the five holes, and
the spatial location of the target was varied randomly from
trial to trial. Each session consisted of 100 trials and lasted
approximately 30 min. Animals initiated each trial by
making a nose-poke response at the food tray. There was
then a 5-s intertrial interval (ITI) during which animals had
to withhold from making a response at the array before the
stimulus light was presented in one of the holes. Premature
or impulsive responses made at the array during this time
period were punished by a 5-s timeout period during which
the house light was turned on and no further trials could be
initiated. A correct response at the illuminated hole was
rewarded with delivery of one food pellet in the food tray.
Food delivery was signaled by onset of the tray light which
remained on until the animal collected its reward. An
incorrect or lack of response (omission) was not rewarded
and instead punished in the same manner as premature
responses. Repeated responding at the correct hole was
classified as perseverative responding and, while moni-
tored, was not punished.
Training took place over 12 stages. For the first stage,
the task was run with the following parameters: 30 s
stimulus duration, 30 s limited hold period (the amount of
time which rats had to make a nose-poke response in the
illuminated hole), and 2 s ITI. These parameters were
gradually adjusted to 0.5 s stimulus duration, 5 s limited
hold period, and 5 s ITI as training progressed. Animals
received 5 sessions per week until a high level of stable
performance (≥80% accuracy, ≤20% omissions) was
reached (67 total training sessions).
The delay-discounting paradigm
The training and testing procedures were very similar to
that described previously (Winstanley et al. 2007). In brief,
animals were initially trained to respond in the middle hole
(hole 3) and then in either hole 1 or hole 5 in order to earn a
reward. Animals were then trained to perform the delay-
discounting task. Each session lasted 56 min and consisted
of 4 blocks of 12 trials, each of which lasted 70 s regardless
of the choice made by the subject. The start of each trial
was signaled by illumination of hole 3, and the rat was
required to nose poke at this hole to trigger illumination of
Page 4
holes 1 and 5. If the rat failed to either respond in the
central hole or subsequently in either holes 1 or 5 within
10 s, the trial was scored as an omission and the box was
returned to the ITI state with all lights extinguished until the
next trial was scheduled to begin. For each rat, responding
in one of either holes 1 or 5 (hole A) always provided a
small immediate reward of one pellet, the other (hole B)
provided a large reward of four pellets. The location of
holes A and B were counterbalanced between subjects.
Each block of trials began with two forced-choice trials. As
the session progressed, the delay to the large reward was
increased in each block of trials in a stepwise fashion, from
0 to 15, 30, and finally, 45 s, whereas the smaller reward
was always delivered immediately. Onset of the tray light
signaled delivery of food reward, and this light was turned
off once the animal responded at the food tray to collect its
reward, at which point the box returned to the ITI state. No
lights were illuminated during the delay to the large reward.
All lights were turned off as soon as a response had been
Acute drug challenges
After stable behavioral baselines were established on each
task, the effects of acute challenge with atomoxetine (0, 0.1,
0.3, and 1.0 mg/kg) and d-amphetamine (0, 0.3, 1.0, and
1.5 mg/kg) were determined. Atomoxetine hydrochloride
was purchased from Tocris Bioscience (Ellisville, MO,
USA). d-Amphetamine hemisulfate was purchased under
an exemption from Health Canada from Sigma-Aldrich UK
(Dorset, England). All doses were calculated as the salt.
Drugs were administered i.p. and dissolved in 0.9% sterile
saline in a volume of 1 ml/kg.
All drugs were prepared fresh daily, and the different doses
were administered according to a diagram-balanced Latin
square design (for doses A–D: ABCD, BDAC, CABD,
DCBA; Cardinal and Aitken 2006, p. 329). Drug injections
were given on a 3-day cycle, starting initially with a baseline
session. The following day, rats received a drug or saline
injection prior to testing. On the third day, animals were not
tested. Injections of amphetamine were given 10 min before
behavioral testing commenced, whereas atomoxetine was
given 30 min prior to testing in accordance with previous
reports (Robinson et al. 2008; Winstanley et al. 2003a).
Animals were tested drug-free for a minimum of 1 week
between compounds to prevent carryover effects.
Ex vivo analysis of brain tissue
Following the last drug challenge, behavioral testing
continued for a further week to ensure that the on-task
drug injections had not affected behavior and that perfor-
mance remained stable. There is no evidence to suggest that
any changes in intracellular signaling caused by such acute
injections should be evident after such an extended time
period. In the unlikely event that brain biochemistry was
permanently altered by this series of drug challenges, such
changes should be evident in both animals treated with
atomoxetine during adolescence and saline-treated controls
and, as such, should not fatally confound our analyses.
Twenty-four hours after the last baseline session, animals
were sacrificed by decapitation. The brains were rapidly
extracted, and the following brain areas were dissected
using brain matrix and tissue punches: the orbitofrontal
cortex (OFC), medial prefrontal cortex (mPFC), nucleus
accumbens (NAC), and basolateral amygdala (BLA).
Tissue samples were frozen immediately on dry ice and
stored at −80°C. Tissue from eight atomoxetine-treated and
eight saline-treated rats were processed for analysis of
mRNA using the real-time quantitative polymerase chain
reaction (qPCR) technique (n=16) or protein levels via
Western blotting (n=16). There were equal numbers of rats
which had learned the 5CSRT and delay-discounting task
within each group of eight, and the groups were matched
for baseline behavioral performance.
Frozen tissue samples were homogenized in the TRIzol
Reagent (Invitrogen, Carlsbad, California). Genomic DNA
was removed from the sample using the DNA-free kit
(Ambion, Austin, TX, USA) and cDNA synthesis was
performed using the SuperScript VILO kit (Invitrogen,
Carlsbad, CA, USA). Transcripts for genes of interest were
quantified using real-time qPCR (SYBR GreenER; Invi-
trogen, Carlsbad, CA, USA) on a StepOnePlus 96-well
thermocycler (Applied Biosystems, Foster City, CA, USA).
All primers were custom-synthesized by Operon (Hunts-
ville, AL, USA; for sequences, see Table 1) and validated
for linearity and specificity. All PCR data were normalized
to levels of glyceraldgyde-3-phosphate dehydrogenase
(GAPDH), which did not vary across treatment groups,
according to the following formula: ΔCt=Ct (gene of
interest)−Ct (GAPDH). Adjusted expression levels for
atomoxetine-treated animals were calculated relative to
their respective saline controls as follows: ΔΔCt=ΔCt
(atomoxetine group)−ΔCt (control group). In keeping with
recommended practice in the field (Livak and Schmittgen
2001), expression levels relative to controls were then
calculated using the following expression: 2−ΔΔCt.
Western blotting
Frozen tissue samples were homogenized in 100–300 μL of
homogenization buffer containing 320 mM sucrose, 5 nM
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer,
Page 5
50 mM sodium fluoride, 1% sodium dodecyl sulfate,
phosphatase inhibitor cocktails I and II (Sigma, P-2850;
St. Louis, MO, USA), and protease inhibitors (Sigma, P-
8340). Protein concentrations were determined using a
Nanodrop 2000 spectrophotometer (Thermo Scientific,
Wilmington, DE, USA) and 50 μg protein was loaded onto
10% Tris polyacrylamide gel for electrophoresis fraction-
ation. The samples were then transferred to a polyvinyli-
dene fluoride membrane and blocked for 1 h in 5% milk in
Tris-buffered saline buffer+0.1% Tween 20 (TBST).
Blocked membranes were incubated overnight at 4°C with
primary antibodies (1/500 phopho-cyclic adenosine mono-
phosphate response element binding protein (CREB), 1/
1,000 CREB, 1/1,000 phospho-MAPK44/42, 1/1,000
MAPK44/42; Cell Signaling, Danvers, MA, USA) in 5%
milk in TBST. After thorough washing with TBST, the
membrane was incubated with the respective secondary
antibodies (1/7,500 to 1/30,000 HRP-conjugated antirabbit
or antimouse IgG; V ector, Burlingame, CA, USA) in 5%
milk in TBST for 2 h at room temperature. Immuno-
reaction was detected using an enhanced chemilumines-
cence system (Pierce Biotechnology-Thermo Fisher
Scientific, Rockford, IL, USA) and quantified by the
Quantity One program (Bio-Rad, Hercules, CA, USA).
The amount of protein blotted onto each lane was
normalized to levels of GAPDH (1:30,000 mouse
antirabbit GAPDH; Research Diagnostics, Concord,
Data analysis
All analyses were conducted using SPSS for Windows
(version 16.0; SPSS, Chicago, IL, USA).
5CSRT Seven variables were analyzed per session: per-
centage of correct responses made (number of correct
responses/total correct and incorrect responses), percentage
of responses omitted (number of omissions/total number
of correct, incorrect, and omitted responses), percentage
of premature responses (number of premature responses/
total number of trials), latency to make a correct
response, latency to collect reward, sum of perseverative
responses made, and trials completed. V ariables that were
expressed as a percentage were subjected to an arcsine
transformation in order to limit the effect of an
artificially imposed ceiling (i.e., 100%). To explore any
differences in task acquisition, the average value for each
variable was calculated for each stage for each rat, and
the effect of drug treatment during adolescence deter-
mined via repeated-measures analysis of variance
(ANOV A) with one within-subjects factor—stage (12
levels, stages 1–12) and one between-subjects factor—
Forward (5′→3′) Reverse (5′→3′)
Adr α1a
Adr α1b
Adr α1d
Adr α2a
Adr α2c
Adr β1
Adr β2
Table 1 The sequence of the
primers used to detect mRNA
for target genes using qPCR
Target genes are listed in
alphabetical order
Adr noradrenergic receptor,
BDNF brain-derived
neurotrophic factor, cdk5
cyclin-dependent kinase 5,
Drd dopamine receptor, NET
norepinephrine transporter, PTB
polypyrimidine tract-binding
proteins, sirt silent information
regulators, TrkB neurotrophic
tyrosine kinase receptor type 2
(receptor for BDNF)


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Launcher Update
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The only really obvious thing is replacing your boots -- I'd suggest the quest from Sha of Anger, except I think you can't do it until 90. But any MoP green plate with int should be an improvement over what you have, almost regardless of precisely what secondary stats are on it.
Other than that, I think you need to be more specific about what the problem is -- is the tank dying faster than you can heal them? (What kind of tank? How much are they pulling? Are they using AM appropriately, or even at all?) Are you seeing a lot of damage to non-tanks that you can't keep up with? Are you getting hit a lot yourself?
Or are you running out of mana? (Is the tank checking your mana between pulls, particularly before bosses?)
Some people have adopted a zerg "strategy" for dungeons that only works if the dungeon is badly undertuned or you outgear it by a lot -- a lot of dungeon groups do fall into one or both of those categories, but some don't and you have to actually learn to play. But it's the whole group that needs to learn to play in that situation, not just the healer. Pulling too many mobs at once, DPS taking too much aggro, or not allowing healers to get mana between pulls can cause serious problems in non-joke dungeons, but there's not much the healer specifically can do about them.
I also request more detail and clarification!
Monastery and Palace are intended to be notable steps up in difficulty from the previous dungeons, as they're a small level bracket jump, but that also means you (and your party) are comparing them to, and maybe believing that they should be as easy, as easier dungeons. When you're doing Brewery at 87, pulling several packs and flying through.. you're out-gearing and out-leveling it. You probably weren't doing that at 85.
Sorry for not clarifying. I can save the tank, not a problem. It is more of AOE healing. When everyone has taken damage I am not sure how to get everyone back up. For instance, the last boss in SPM. There is a lot of damage that goes out and it becomes tough for me. This is my first healer so I just get a little overwhelmed and panic I guess.
90 Blood Elf Paladin
EDIT: I main-spec holy - just realized i logged out in prot for somebody to look at something.
Lots of damage on last boss in SPM.. means that people are doing 1 of 2 things (or both)
1) Orbs - They were ignored by dps or dps couldn't kill them fast enough and people stay standing in the purple puddle
2) Swirly wall of ouch centered on the boss - people standing in that.. it hurts...
Things you can do.
1) Beacon tank or if you have 1 good dps you're identified beacon them and keep healing the tank (but don't forget about yourself). Try to get a EF running everybody (even if its just a 1 or 2 hp one).
2) Pretty sure Devo helps on that fight (I really haven't had to use it to know).
3) Yell at dps/tanks standing in the purple.
4) Don't be afraid to switch Divine Purp for HA for a fight. Its duration is long enough to get a 3HP EF running on everybody.
5) If people are spread out then move beacon around and heal somebody else. Spreading out negates the holy radiance and LOD healing big time.
Edited by Mechvfpally on 8/12/2013 1:45 PM PDT
AoE means using radiance/LoD, and then;
If you've identified a dps during the course of a run that's problematic, low dps, always standing in things, feel free to have them be your lowest priority to heal and don't feel bad if they die.
You can heal a lot, but you can't heal stupid, and it's not your responsibility to do it forever. A healer can only be expected to do so much, and if everyone is taking damage from something that they shouldn't be taking damage from, that's not entirely on you. They need to not be in it, or not be doing what caused it.
Edited by Cayse on 8/12/2013 2:00 PM PDT
I was going to suggest the same thing. If there's one DPS that is particularly bad at taking damage (like taking more than the other two DPS combined), use only cheap heals on them when nobody else needs healing, and if that results in their death, 4-man the encounter and then raise them. (Some people wouldn't even do the raise part.) If they complain, point out that they were taking much more damage than the other DPS and ask why. Chances are, one of the other DPS or the tank will come in and point out that they were standing in fire or the equivalent.
At that point the problem DPS can either shape up, shut up, or get lost -- and it doesn't really matter which. (Although, in this case, it was the endboss anyway.) DPS in general are expendable in a lot of content, but especially DPS that don't avoid avoidable damage. You should try to avoid blaming yourself for other people's mistakes (or letting other people blame you).
I wonder if it is a CD issue, as in not using them. I could see you getting up to these two dungeons without having to use anything but mana and direct heals.
Paladins are reactive healers so anything you can do proactively helps.
Devotion aura usually works on "purple" Hand of sacrifice. Rebuke, repentance, stun, dispells all these things can reduce what you have to heal .
90 Blood Elf Paladin
I wonder if it is a CD issue, as in not using them. I could see you getting up to these two dungeons without having to use anything but mana and direct heals.
As soon as you mentioned Shado-Pan Monastary, I immediately thought of Devotion Aura. I remember early in the expansion trying to get through heroics; once I remembered about Devotion Aura, things started to get a lot easier. Then don't forget to use Guardian of the Ancient Kings for that extra healing boost. It's still a 5-man, so don't worry about only pumping Divine Lights off of it-- whatever heal you can get off in time to save people without sucking your mana dry is always a good heal.
You've also got your personal magic bubble to help relieve yourself from trying to heal yourself above others. Then there's also Hand of Sacrifice (which has been one of my favorite spells ever), to help cushion a health bar from dropping too quickly.
It's the last boss of the dungeon, so don't be afraid to use your cooldowns. At least you remember that they exist.
Also you're under 8-9k Spirit, so Glyph of Illumination might help out of mana is an issue. I've been hearing things about it, like dropping your mana by 15% when zoning in and stuff but then again, I don't know many people who take it on account of their spirit levels.
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