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Where has liberty gone
Posted By Editor On January 3, 2013 @ 7:17 am In Opinion/Commentary,Political & Politicians | Comments Disabled
The Bill Of Rights Has A Very Bad Week [1]
By Charles P. Pierce [2]
[3]Of course, while everyone in Washington, and the courtier press that serves them, were endlessly droning on and on about the Gentle Fiscal Incline, the Bill Of Rights closed out 2012 by having one of the worst weeks it's had in the two centuries of its existence. But the courtier press paid that little mind, possibly because selling out the Bill Of Rights was done on a "bipartisan" basis, and the denizens of the various Green Rooms would endorse cannibal murder if both parties agreed to subsidize it.
First came the revolting vote on the reauthorization of FISA. Time was, and not that long ago, that the whole idea of a secret court issuing secret warrants was enough to raise hackles all on its own. (There even was an episode [4]of Law And Order from 2004 that centered on the execution of a FISA warrant that sent liberal defense attorney Danielle Melnick, and assistant DA Serena Southerland, up the wall.) Now the old FISA regulations seem like they were drafted by George Mason, compared to what keeps getting reauthorized in the Senate.
This latest thing was to reauthorize the truly spooky FISA Amendments that were passed in 2008 when the president, in one of the actions he's taken that really was a naked sellout of his previously enunciated principles, joined with a Senate majority to immunize the telecommunications companies that had participated in the Bush Administration's lawlessness regarding wiretapping, as well as to authorize sweeping new wiretapping powers far beyond those against which the companies were being immunized. What the president did is not excused by the fact that he was running for president at the time. This wasn't a flip-flop he took because he wanted to be elected. This was a flip-flop he took because he wanted to do some things once he was elected.
This year, as those amendments came up for reauthorization, what we had was a genuine horror show, and it was made worse by the awful debate that preceded the vote. There was enough boogedy-boogedy coming from the Democratic side to embarrass John Bolton. Glenn Greenwald already has written at length [5] on this, but one comment from the now-inexcusable Dianne Feinstein is worthy of further comment.
"This is an effort to make that material public, and I think it's a mistake at this particular time because it will chill the program, it will make us less secure, not more secure…I know where this goes, it goes to destroy the program. I don't want to see it destroyed."
In other words…no, wait, there are no other words. This is the argument of a totalitarian. You can't know what we're doing to protect you, even if we're doing it to you, because then we can't protect you and you will be killed by bad people and it will be your own fault. Somewhere in East Germany, an elderly ex-bureaucrat is getting a thrill up his leg and doesn't know why.
Later, came the release of some FBI documents [6]in which it seemed to indicate at least an unacceptable level of involvement by federal law enforcement in the crackdowns by local authorities on the various outposts of the Occupy movement. …
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URL to article: http://www.berthoudrecorder.com/where-has-liberty-gone/
URLs in this post:
[1] The Bill Of Rights Has A Very Bad Week: http://www.esquire.com/blogs/politics/bad-week-for-the-bill-of-rights-123112
[2] Charles P. Pierce: http://www.esquire.com/archives/blogs/politics/by_author/68/15;1
[3] Image: http://www.shestokas.com/constitution-educational-series/bill-of-rights-of-the-us-constitution-promise-made-promise-kept-2/
[4] was an episode : http://www.imdb.com/title/tt0629206/
[5] already has written at length: http://www.guardian.co.uk/commentisfree/2012/dec/28/fisa-feinstein-obama-democrats-eavesdropping
[6] the release of some FBI documents : http://www.justiceonline.org/commentary/fbi-files-ows.html#documents
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2008 Mazda 3 Mazdaspeed 3: 12.793 @ 106.900 driver311, Tires: m@h 24.5x8.5x15 inch slicks
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Need Cabin Mate
Hello SWM in need of cabin mate departing New Orleans Nov 27, 7 day Freeport Nassau Keywest Cost $ 459.00 I will be driving in from Houston if you need a ride to save on gas and parking. Thanks Mark:cool::cool::cool:
Thinking of going myself.
Mark--I submitted before I finished my reply. I was thinking of going myself, if I can get vacation at that time. SWM, 48, live in N.O.
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ANSYS Closes Acquisition Of Reaction Design
PITTSBURGH and SAN DIEGO, Jan. 3, 2014 (GLOBE NEWSWIRE) -- ANSYS (Nasdaq:ANSS), a global leader and innovator of engineering simulation software, announced today that it has completed the acquisition of Reaction Design, a leading developer of chemistry simulation software. The Company will provide further details regarding the transaction and its impact on the 2014 financial outlook at the time of its 2013 year-end earnings announcement.
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The combination of ANSYS' proven computational fluid dynamics (CFD) solutions with Reaction Design's industry-leading chemistry solvers will provide the best-in-class combustion simulation tools available on the market. Understanding and predicting the effects of chemistry in a combustion system is critical to developing competitive products in transportation, energy and materials processing applications. Relying on experimental testing alone for accurate performance validation is inadequate, given today's complex designs and shortened design cycles. Effective simulation of the underlying detailed chemistry is critical to advancements in engine and fuel technology. Reaction Design's solutions enable transportation manufacturers and energy companies to rapidly achieve their clean technology goals by automating the analysis of chemical processes via computer simulation and modeling solutions. The acquisition of Reaction Design complements ANSYS' offering for the simulation of internal combustion engines, leading to improved fuel efficiency.
To learn more about ANSYS and the acquisition of Reaction Design, please visit www.ansys.com . About Reaction Design Reaction Design, a San Diego, California-based software supplier, enables transportation manufacturers and energy companies to rapidly achieve their clean technology goals by automating the analysis of chemical processes via computer simulation and modeling solutions. Reaction Design is the exclusive developer and distributor of CHEMKIN and CHEMKIN-PRO, the gold standard for modeling gas-phase and surface chemistry that provides engineers ultra-fast access to reliable answers that save time and money in the development process. Reaction Design's FORTÉ is an advanced computational fluid dynamics (CFD) simulation package for realistic 3D modeling of fuel effects in internal combustion engines with superior time-to-solution metrics that fit in commercial development timeframes. Reaction Design's ENERGICO software brings accurate chemistry simulation to gas turbine and boiler/furnace combustion systems using automated reactor network analysis. Reaction Design also offers the CHEMKIN-CFD software module, which brings detailed kinetics modeling to other engineering applications, such as CFD packages.Check Out Our Best Services for Investors
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Latex Slaves
Watch as the latex sexcapades unfold between slaves and mistress's in this other dimension of submission. Once you've tuned in you will never want to tune out.
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Dynamic Materials Corp. 5405 Spine Road Boulder, CO 80301 United States - Map Phone: 303-665-5700 Fax: 303-604-1897 Website: http://www.dynamicmaterials.com
Dynamic Materials Corporation, together with its subsidiaries, manufactures explosion-welded clad metal plates, and oil and gas well perforation products; and provides welding services worldwide. It operates in three segments: NobelClad, DynaEnergetics, and AMK Technical Services. The NobelClad segment provides explosion-welded clad metal plates that are used in the construction of heavy, corrosion resistant pressure vessels and heat exchangers for oil and gas, alternative energy, chemical and petrochemical, hydrometallurgy, aluminum production, shipbuilding, power generation, and industrial refrigeration industries. The DynaEnergetics segment manufactures, markets, and sells perforating explosives, and associated hardware and seismic explosives for the oil and gas industry. It offers detonating cords, detonators, bi-directional boosters, shaped charges, perforating guns, sub-assemblies, systems, and seismic related explosives and accessories to large, mid-sized, and small oilfield service companies in the United States, Europe, Canada, South America, Africa, the Middle East, and Asia. The AMK Technical Services segment provides welding and machining services principally to power turbine manufacturers, and commercial and military aircraft engine manufacturers. The company was formerly known as Explosive Fabricators, Inc. and changed its name to Dynamic Materials Corporation in 1994. Dynamic Materials Corporation was founded in 1965 and is headquartered in Boulder, Colorado.


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Gulf Coast Leadership Institute seeks top 'dawgs'
Date: March 18, 2013
by: Alex Mahadevan | News Editor
Gulf Coast Community Foundation is accepting applications for a summer leadership-training program through 5 p.m. Friday, March 22.
The philanthropic organization will pick up to 25 emerging leaders for the Gulf Coast Leadership Institute based on their potential, desire and involvement in community or professional organizations. The students will participate in seven training sessions from April through June, through the J.W Fanning Institute for Leadership Development at the University of Georgia.
The program includes classes on conflict management, the value of community diversity, communication and group decisions. Training sessions take place every other Friday, starting April 19, until a June 21 graduation.
Contact Alex Mahadevan at firstname.lastname@example.com.
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28 Faith Preschool's Annual Kid's Carnival and Silent Auction
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Seventh-graders at R. Dan Nolan Middle School put conviction to action Monday, as they installed a sign at Lakewood Ranch High School.


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Books about Chinese culture and studies, literature, journalism, history, philosophy and art.
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EPP in the News
EPP will be exhibiting at ACRL, March 25-28 in Portland
We are excited to be exhibiting at the upcoming Association of College Research Libraries (ACRL) conference in Portland,March 25-28, 2015. Please drop by booth 782 and say hello! Chinas economic growth
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EPP Attends Beijing Book Fair, August 2014
EPP Booth at Beijing Book Fair EPP was invited to attend a Publishing Agreement Signing Ceremony for an author, Wu Wei, who was a former chief of China News Bureau, named the China Success. The book wi


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From Wikipedia, the free encyclopedia - View original article
Type | Public |
Traded as | DSE: DUTCHBANGL |
Industry | Banking |
Founded | Dhaka, Bangladesh (1995) |
Headquarters | Dhaka, Bangladesh |
Key people | M Sahabuddin Ahmed -Founder & Chairman; Financierings-Maatschappij voor Ontwikkelingslanden (FMO) |
Products | Banking services, ATM services, Consumer Banking Corporate Banking Investment Banking |
Employees | 1600 |
Website | [1] [2] |
This article needs additional citations for verification. (April 2011) |
Type | Public |
Traded as | DSE: DUTCHBANGL |
Industry | Banking |
Founded | Dhaka, Bangladesh (1995) |
Headquarters | Dhaka, Bangladesh |
Key people | M Sahabuddin Ahmed -Founder & Chairman; Financierings-Maatschappij voor Ontwikkelingslanden (FMO) |
Products | Banking services, ATM services, Consumer Banking Corporate Banking Investment Banking |
Employees | 1600 |
Website | [1] [2] |
Dutch-Bangla Bank Limited (the Bank, DBBL) is a scheduled joint venture commercial bank between local Bangladeshi parties spearheaded by M Sahabuddin Ahmed (Founder & Chairman) and the Dutch company FMO. DBBL was established under the Bank Companies Act 1991 and incorporated as a public limited company under the Companies Act 1994 in Bangladesh with the primary objective to carry on all kinds of banking business in Bangladesh. DBBL commenced formal operation from June 3, 1996. The Bank is listed with the Dhaka Stock Exchange Limited and Chittagong Stock Exchange Limited.
DBBL is most widely recognized for its donations to social causes and its IT investment (largest ATM network). However it has recently[when?] stated that it will stop expansion on its ATM network as the current numbers have exceeded demand and hence diminishing returns (if any). Although it is widely believed it is a loss-making/subsidized unit which DBBL rationalizes as quasi CSR.
The bank is often colloquially referred to as "DBBL", "Dutch Bangla" and "Dutch Bangla Bank".
Contents |
After instability and frequent management changes in its initial years, DBBL overcame these obstacles to establish rapid growth since the year 2000. The bank grew its reputation through social work rather than profits. The bank's conservative nature, long-term strategies, hefty social donations and technology investments have always led to modest but steady profits. DBBL has been known to be overly conservative in its banking practices. Much of the success and strategy has been attributed to the leadership of the founder chairman, M Sahabuddin Ahmed.
Despite being the largest corporate donor in Bangladesh, investor confidence was unhindered. In March 2008, DBBL share prices reached Tk. 14325.80 in the Dhaka Stock Exchange, setting the record for the highest stock price in the history of Bangladesh. It is also one of the few banks that does not participate in merchant/investment banking (which can lead to sporadic growth).
Dutch Bangla Bank is noted to be the first and only local bank in Bangladesh to have an automated banking system. The bank has spent over BDT 2 Billion in automation upgrades (first bank in Bangladesh to do so). This automation took place in 2003 whereby services of the bank were available uniformly though any branch, ATM and internet. Banking was a paper based until DBBL, with its wide local network, delivered banking automation and modern banking services to the masses. This effectively introduced the 'plastic money' concept into the Bangladeshi society. The entire automation system was imported and is the most expensive banking related IT project in Bangladesh.
The ATM count stood at 140 eclipsing other networks by a large margin. This rapid increase in DBBL's ATM network has led the majority of other banks to sign sharing agreements instead of pursuing their own ATM network.
In 2006, Dutch Bangla Bank ventured into the realm of personal credit by offering automatic credit facilities to its Debit card (DBBL Nexus) holders. It also continued expanding its ATM network with the intention of installing more than 400 machines, even though it is widely believed that the ATM division will not break even and is a setback to the profit margins.
In 2007 the bank entered a strategic alliance with Citibank NA, where both parties will provide retail banking services of DBBL for its employees and owners of Citibank corporate customers in Bangladesh. In the same year DBBL became the first bank in Bangladesh to offer SMS Banking services.
DBBL maintained a proprietary policy on its ATM network until 2007. This was changed when DBBL and Standard Chartered Bank (SCB) signed an agreement that will let SCB use DBBL's ATM network. Thus ending the unhealthy competition between the organizations. This sharing invitation was extended to all other banks shortly after. Number of DBBL ATMs eclipsed the 200 mark.
In 2010 most sharing agreements with foreign banks have been abruptly rendered invalid due Visa card's new policies enforced upon DBBL. In response to Visa's decision to terminate its ATM facilities with DBBL, Unionpay approached DBBL to convert all the retail network to accept Unionpay cards. DBBL and Unionpay have decided for a major nationwide launch to make Unionpay the single largest card issuer in Bangladesh.
DBBL maintains its own network and automation without the involvement/assistance of any third party companies. Access to ATM services fee is BDT 230 annually for DBBL clients. Partner banks are charged a subsidized Tk. 10, although the end price for the customer may be higher due to the extra charges levied by the partner bank.
DBBL has over 870 ATM's installed all over Bangladesh, making it the largest network by a large margin. In 2009 DBBL announced the biggest its biggest expansion goal for its network. The total number of ATMs will be 850 by the end of 2009 to cater the needs of customers at their doorsteps.[1] It also hinted that this may be the last ATM expansion due to the increasing costs of subsidizing such a system, but clearly stated that it has no intention of introducing any charges.
On the digital day (10/10/10), the DBBL inaugurated its (digital) 1000th ATM at the factory premises of the GlaxoSmithKline, Chittagong.(as on 24th March 2012 it has more than 2000 ATM and 200 FT)
Banks that have signed agreements to share DBBL's ATM network (as of May 2009):
Dutch-Bangla Bank initiated a co-branded ATM initiative in Bangladesh where the bank would provide ATMs and networking free of charge to any bank's branches. Mutual Trust Bank was the first bank to take advantage and the first co-branded ATM in Bangladesh started operation on 28 May 2008.
In 2008, Dutch-Bangla Bank started offering full banking automation services to other banks where without any investment or technical knowledge, member banks can automate themselves in matter of months using DBBL's infrastructure (this includes required hardware, card issuance, ATM usage & switching software).
During the Q1 of 2010, Dutch Bangla Bank Ltd. introduced "Fast Track" in the country. Fast Track is first of its kind in the country that is like a mini branch. Along with the generic ATM withdrawal service, it lets the customer deposit small amounts of money to DBBL account! The limit is currently 20,000 BDT. Moreover, the Fast Track also provides account opening service and loan information. Presently the number of First Tracks is more than 200 and the number of ATMs is more than 2000 all over in Bangladesh[2][unreliable source?] (As on March 24, 2012).
On 3 June 2010, Dutch Bangla Bank Ltd. announced to allow internet payments system. Using their Internet Payment Gateway merchants will be able to charge their customers' Visa, Masters, DBBL Nexus and Maestro cards. This is going to be revolutionize the [e-commerce] in Bangladesh! Presently DBBL has more than 200 of Frast Tracks and more than 2000 ATMs all over in Bangladesh[3]
Any Visa/MasterCard cardholder (local or overseas) can use their cards to pay at a number of e-Merchants against their purchase of goods. They can also pay DESCO electricity bills. DBBL is working with airlines, railways, utility companies, educational institutions, and stock exchanges for facilitating purchase of tickets, payment of bills/fees and IPO subscription through the Internet Payment Gateway. It is also working to make an interface with PayPal. Once everything is in operation, this will change the payment mechanism in Bangladesh and will reduce public sufferings and tensions.
Dutch Bangla Bank supports social work extensively and is one of the largest private donors in Bangladesh. Under the vision of M Sahabuddin Ahmed, the bank contributes extensively through its foundation Dutch Bangla Bank Foundation. This has been one of the biggest priority for the bank since its inception. As a result the organization has been granted numerous national and international awards for its role in the development of Bangladesh. The Dutch-Bangla Bank Foundation was established under the vision of its founder chairman, M Sahabuddin Ahmed, in order to help the people of Bangladesh. DBBL is the largest corporate donor in Bangladesh with total donations exceeding BDT 1.3 billion.
The bank donates mainly towards social awareness programs, medical and educational fields. Notably DBBL also maintains the largest scholarship program in Bangladesh whereby college/university education tuition and expenses are fully paid for unconditionally by the bank.
In 2007, the bank won the Bangladesh Business Awards for being the 'Best Financial Institution' mainly for its commitment to technology and community service. In 2007 alone, DBBL donated BDT 220 million towards social causes.
In 2008, DBBL announced a donation of Tk 100 million to fund the construction of a 11 storey research center, titled "Dutch-Bangla Bank Research Centre for Advanced Research in Arts and Social Sciences" at the Dhaka University campus in Dhaka, Bangladesh. It was Dhaka University's largest ever donation from a private company. The research center, which would be the first of its kind in Bangladesh, will be supervised by renowned teachers, scholars and researchers. It will also award scholarship to researchers and scholars at home and abroad.
In February 2011, it was announced that local sponsoring shareholders will not be receiving dividends to "achieve greater things". In May 2011, DBBL announced the largest scholarship program in Bangladesh where DBBL would have to donate Tk 102 crore every year. DBBL was already the single largest corporate donor in Bangladesh and this new announcement had surprised many, especially because it came from such a small bank. The structure of this scholarship had greatly benefited students who had the academic performance, but required the financial aid.
Corresponding this announcement, DBBL published the first group of awardees in a 10-page advertisement spread in multiple newspapers. The recipients were awarded in a ceremony where the stadium being used was not big enough to hold all the guests. Speakers at this event greatly thanked DBBL's founder chairman, M Sahabuddin Ahmed, for his vision and dedication in creating opportunities for students.
Dutch-Bangla Bank Limited (DBBL) has for the first time introduced its mobile banking service, expanding the banking service from cities to remote areas. Bangladesh Bank Governor Atiur Rahman inaugurated the service in July, 2011 by depositing Tk 2,000 and withdrawing Tk 1,500 through Banglalink and Citycell mobile networks in Motijheel area. Bangladesh Bank has already allowed 10 banks to initiate mobile banking. Of them DBBL kicked off first. "Mobile banking is an alternative to the traditional banking through which banking service can be reached at the doorsteps of the deprived section of the society," the central bank governor said at an inaugural press briefing at Hotel Purbani. Atiur Rahman said through mobile banking various banking services including depositing and withdrawing money, payment of utility bills and reaching remittance to the recipient would be possible. By going to the DBBL-approved Citycell and Banglalink agents throughout the country, the subscribers can open an account provided they show the necessary papers and pay a fee of Tk 10. To use the banking service, subscribers must own a cell phone from any provider. The bank gives subscribers a four-digit PIN. By using the PIN, subscribers can use a number of banking services, including depositing and withdrawing money, while maintaining account security. Customers may hand over cash to agents in the bank's network, and agents can coordinate the transaction from their mobile phones, helping account holders securely complete banking tasks using their PINs. Customers can deposit or withdraw money up to five times per day, up to Tk 5,000 per day. One percent of the transaction account or Tk 5, whichever is greater, will be deducted as a cash-in charge. The charge for cash-out is 2 percent of the transaction amount or Tk 10, whichever is greater. There are no fees for registration, salary or remittance disbursement services.


text:
CW12 Directory ResourceArticle Details
Information On Handcrafted Kitchens |
Category: Computers & Internet: Web Directories: Niche Directories: Gambling |
Many people imagine that having handmade kitchens is really a luxury. That thought truly is
incorrect and is something of the past. Whilst having a hand-crafted
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apparent extra cost that comes with it. Hand made kitchens need more than design and
creativity. One also needs to think about the elements which will be used to put the kitchen together. There's
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Many people
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use such a kitchen everyday, specifically Mothers
who cook for their family will probably be in a happier state of
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Just to summarize the very first statement within this entry, hand made kitchens aren't
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Additionally, you will no longer have to be concerned about installation. If you work with a
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There will definitely be quite a few factors which you need to consider when
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need to remember the most significant factors. Consider your
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Soon after deciding on these items, you can then pursue a designer, or a business
who would willingly work with you in coming up with your dream kitchen.
Date Added: November 26, 2012 11:29:35 AM |
Author: Mai Langer |


text:
Corporate Finance Services
Guiding you through your business transactions.
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State of the Union transcript 2011: Full text of the president's speech
State of the Union transcript 2011: President Barack Obama delivered his State of the Union address to Congress on Tuesday night. Here is the full text of the speech.
Pablo Martinez Monsivais/AP
THE PRESIDENT: Mr. Speaker, Mr. Vice President, members of Congress, distinguished guests, and fellow Americans:
Tonight I want to begin by congratulating the men and women of the 112th Congress, as well as your new Speaker, John Boehner. (Applause.) And as we mark this occasion, we're also mindful of the empty chair in this chamber, and we pray for the health of our colleague -- and our friend -– Gabby Giffords. (Applause.)
It's no secret that those of us here tonight have had our differences over the last two years. The debates have been contentious; we have fought fiercely for our beliefs. And that's a good thing. That's what a robust democracy demands. That's what helps set us apart as a nation.
But there's a reason the tragedy in Tucson gave us pause. Amid all the noise and passion and rancor of our public debate, Tucson reminded us that no matter who we are or where we come from, each of us is a part of something greater -– something more consequential than party or political preference.
We are part of the American family. We believe that in a country where every race and faith and point of view can be found, we are still bound together as one people; that we share common hopes and a common creed; that the dreams of a little girl in Tucson are not so different than those of our own children, and that they all deserve the chance to be fulfilled.
That, too, is what sets us apart as a nation. (Applause.)
Now, by itself, this simple recognition won't usher in a new era of cooperation. What comes of this moment is up to us. What comes of this moment will be determined not by whether we can sit together tonight, but whether we can work together tomorrow. (Applause.)
Page 1 of 23


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Some individuals may migrate to the surface at night (Ref. 27000). Minimum depth reported taken from Ref. 27000. Feed mainly on myctophids (Ref. 47377).
- Gibbs, R.H. Jr. 1990 Stomiidae. p. 296-299. In J.C. Quero, J.C. Hureau, C. Karrer, A. Post and L. Saldanha (eds.) Check-list of the fishes of the eastern tropical Atlantic (CLOFETA). JNICT, Lisbon; SEI, Paris; and UNECO, Paris. Vol. 1. (Ref. 4464)


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Moore:Ribosome Purification
From OpenWetWare
Contents |
Ribosomes are large aggregates of RNA and protein. Usually, when you harvest E. coli to get them, most of the ribosomes are in the process of making a protein, so what you end up with are ribosomes with some tRNA, mRNA, and translation factors on them. For some studies, you'll want to find a way to remove these components, see (protocol). In general, they're pretty robust, but you can ruin them if you make some mistakes:
- Removing the magnesium, either by dilution or using a chelator, will perma-break them.
- Letting RNAse chew on them. As mentioned below, use a strain lacking RNase I (rna).
- Heating them over 50 °C, up to and including 50 is fine if the solution is properly buffered.
- Letting the pH move far away from ~7.5. High pH causes RNA hydrolysis, especially when metal is present.
- Metal-induced RNA cleavage. Keep iron chelated with a small amount of EDTA.
Medium, Growth, and Harvest
- Early protocols call for growing E. coli in a high-phosphate medium. I usually use LB with 0.2% glucose, they seem to be fine.
- Rapidly chill the cells on ice when they are in early- to mid-log phase. If you let let the cells cool slowly (like in a cold room or centrifuge) they will cold-adapt and synthesize proteins that shut down translation. If you want to be serious, you can add ice directly to the culture. Don't use ice from tap water, generally, a frozen buffer of some sort is used, about = volume to the culture. Keep all of your stuff on ice until lysis.
- Harvest the cells in a 4 °C centrifuge, and resuspend in a washing buffer, re-harvest, then resuspend in a lysis buffer. Make the cells concentrated, resuspending a liter of log-phase bugs in 3-5 mls of lysis buffer works pretty well.
Lysis and Removing Cell Debris
- You can use a pressure cell to shear the cells open. Works fine for huge preps, but is rather impractical for most volumes.
- I either use (1) hen egg white lysozyme, or (2) phage λ lysozyme. See: Sauer:Lysing_E._coli_with_Lysozymes. I generally grow the cells, and initiate lysis, divide the lysate into micro-fuge tubes after the DNase reduces the viscocity, and spin out most of the cell debris (~10 min, full speed), pipette the supernatants to clean tubes, then freeze the aliquots at -80 °C.
- It is a good idea to spin a few times before pelleting the ribosomes in the ultracentrifuge: there will be chunks of membranes that contaminate your ribosome pellet. So, the day you plan to pellet the ribosomes, thaw the lysate aliquots and spin them again, or two more times, each time removing the sup to a clean tube. Keep your stuff on ice.
Making "Crude" Ribosomes by Pelleting Them
Here, the goal is to separate te ribosomes from the majority of other, smaller things in the cell. The lysates are layered onto buffer solutions in centrifuge tubes containing 10% sucrose to make it dense. The lysate will float on the sucrose, but when you spin the solutions under high g-forces, the ribosomes out-run most of the other stuff to the bottom of the tube.
How long do I spin them?
This depends on what centrifuge/buckets you're using, how thick your sucrose layer is, what temp the solution is, and how fast you plan to spin them. You can calculate or look-up the "k-factor" for your rotor (a convenient term from Beckman-Coulter that relates spin time to what you want to pellet).
- If you don't know or can't find the k-factor:
k = ln(rmax/min)/ω2 x 10-13/3600
- If you know a k-factor, but don't want to spin at speed for which it was calculated:
knew = kcurrent x (speedcurrent/speednew)2
- If you have a k-factor and want to know how long to spin:
t (in hours) = k/S
E. coli ribosomes are 70S, the subunits are 30S and 50S. If you want all of the material to pellet, calculate to pellet 30S.
- Note: You are spinning through sucrose at 4 °C. This is more viscous than water (for which the k-factor is calculated). You have to increase the spin time because the material moves quite a bit slower through the sucrose. 8% sucrose is about twice as viscous as water (I forget where I read that, I'm getting old). I double the time needed to pellet 30S, or calculate the time needed to pellet 15S (same thing). This does pretty well, most rRNA is in the pellet and most tRNAs and SsrA remain in solution.
The first few times you pellet ribosomes in whatever rotor you choose, check where the material distributes by purifying RNA from each phase and running a gel examples of E. coli RNAs.
Resuspending the Ribosomes
- After your spin, you will, in most cases, want to discard the supernatant. Aspiration works well, draw from the meniscus and follow the solution down to the pellet. Suck it dry. If you did a good job of pre-clearing your lysates, the ribosome pellet will be a crystal clear "lense" that has the consistency of wax.
- Add a buffered solution containing magnesium to the pellet (I use HT-6). Don't touch the pellet with your pipette tip, it is really sticky. I usually resuspend pellets in ~200 μl of buffer. Place the tubes in a rack and shake then at 4 °C until the ribosomes are in solution. To speed things up, I make mini stir-sticks from pipette tips: put a 200 μl tip on a pipetter, pass it through a flame just until it lights, then blow it out. The tip will seal shut. Rinse the tip in and aliquot of ribosome buffer, and eject it in to the centrifuge tube. Having the strirrer in the tube reduces resuspension time significantly.
- When the pellet is gone, transfer the solution to a clean tube and spin about ten minutes to remove any large bits and membrane contamination. Transfer the ribosomes to a new tube. Highly-concentrated solutions at this stage will be turbid and may have a yellow/orange tint from NADH bound to contaminating pyruvate dehydrogenase (this enzyme complex is huge, about 50S, but doesn't seem to interfere with anything ribosome-related).
- Quantify the ribosomes by diluting in a buffer and measure the A260. See: Link that has ε for rRNAs
- Freeze at -80 °C.
- Most plasticware in the lab binds proteins and nucleic acids. Avoid using tubes and tips that haven't been tested for binding. Also, keep a non-ionic detergent in your solutions (I use Tween-20). I have tested a few brands of tubes for ribosome binding, some are horrible. I currently use Axygen "Maxymum Recovery" tubes.
- Make sure you don't touch anything that touches your buffers, tips, tubes, etc. with bare hands, directly, or indirectly. You WILL introduce RNase that will degrade your stuff.
- Having EDTA in solutions with magnesium seems odd, I know. However, EDTA has a much higher affinity for many other metal ions. It is used to scavenge trace metal that can cause RNA damage (like iron) from anything your ribosomes are in.
Buffers and Solutions
These are solutions I use, other groups use different solutions. I avoid the use of sodium and chloride: these aren't present in high levels in the cell and NaCl will inhibit translation reactions. High magnesium (5-50 mM) promotes subunit association into 70S particles, low magnesium (~1 mM) allows subunit dissociation. This process is dependent on the concentration of ribsomes as well, at very low ribosome concentration, the subunits will mostly be dissociated.
Cell Wash Buffer
- 20-50 mM Buffer, pH ~7.5. I use 40 mM K+-HEPES, pH 7.4.
- Salt. I use 100 mM NaCl.
- Magnesium to match what's in your lysis buffer.
"HT" Stock Buffer
This is a "3X" solution used as a base for a lot of buffers I make.
- 300 mM K+-glutamate.
- 60 mM HEPES-Tris. Measure for 300 mM HEPES and add Tris base to raise the pH to 7.5.
- 0.3 mM EDTA
Filter and store at 4 °C.
- 1/3 vol HT Stock Buffer
- 6.1 mM Mg-OAc (from 1M stock that has 1 mM EDTA).
- 14 mM 2-mercaptoethanol (just use 1/1000 of stock).
- 0.05% Tween-20 (from 10% stock solution).
This buffer can grow stuff. Glutamate is unstable. Don't use old buffer, keep what you have at 4 or -20 °C.
10% Sucrose
Make HT-6, but with 10% sucrose as well.
Lysis Buffer
- HT-6 with:
- 10 U/ml DNase I (Don't use crappy stuff, use RNase-free, molecular biology grade)
- 0.5 mM CaCl (DNase I needs calcium)
- 0.1 mg/ml lysozyme (only if using exogenous lysozyme)


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The Fox and The Frog"Stay back!" Astra snarled, backing up further into the alley. To her chagrin it was a dead end, and left her little to no escape from the 'mons that were ahead of her.A Medicham and Drowzee approached slowly, both wearing menacing grins, as they looked the Braixen over. The two shot glances at each other and then turned their gazes back to Astra who bared her fangs and snarled viciously."You know I bet some witch hunters would pay a pretty fortune for an infamous Witch like you," snickered the Medicham, as he shifted his arms to his waist, "a pretty price for a pretty witch," he commented with a laugh, which was accompanied by the Drowzee's."Keep your crummy hands to yourselves! I'm warning you!" Astra spat. She moved back, still relying on her trusty staff for balance."You're surprisingly good at running away for having to use a crutch, too bad you're no where
A brand new feeling-Quick Caption"Ugh this is embarrassing." Steven said walking the red floors of the Lampton hotel.He and his buddy, Cole, were on their way to the private hut tub they had rented out for the weekend. But they weren't in normal male swimming attire. They were wearing bikinis that hugged their bodies and outlined their male instruments in abnormal proportions. Steven wore a red bikini and Cole wore a green one."You didn't have to agree with this pal. No one forced you." Cole explained.Steven sighed. "Why do you guys do this again?" "Because if feels...you'll see. Did you spray the stuff on you?" Cole asked."Yeah I did.""You sure?"Steven threw his head up in the air. "Yes Cole I did. I sprayed down every inch of my body yeesh.""Alright alright just wanted to make sure. It's the only way this works.""Yeah, yeah." The boys approached the outside exit and walked onto the
Out Of Place CHAPTER 5CHAPTER 5MEANWHILE Slowly Donnie opened his eyes. He was lying on the couch, next to him Leo and Raph were staring at him. Taking several deep breaths Donnie spoke. "W.....what happened?"It was Raphael who spoke first, his tone confused and slightly worried. "You tell us egg head. One moment everything is fine, and the next the entire lair shook and smoke started coming from your lab. We had to carry out because you were unconscious."Blinking Donnie groaned and slowly sat up. Every part of his body ached, as though he'd been a fight and gotten his shell handed to him. Moving slowly he shifted, reaching up to rub his head. What had happened? One moment he'd been……..Donnie's eyes opened before scanning the area around him. "Where's Mikey?"Raph raised an eyeridge. "Huh?"Biting his lip Donnie spoke again. "Mikey…..he was in the lab with me. W….where is he now?"H
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text:
Groupe Auchan bears the name of its historic business, the hypermarket, opened in 1961 by Gérard Mulliez. Today it is a federation of autonomous, responsible and enduringly prosperous companies, developing around a shared mission:
"To improve the purchasing power and the quality of life of the greatest number of customers, with responsible, professional, committed and respected employees."
This mission is based on three fundamental values: trust, sharing and progress.
Groupe Auchan operates in 16 countries, employs 302 500 people (on 31/12/2013, average payroll, FTE) and generated in 2013 €62.1 in revenue including taxes for the chains.
Groupe Auchan's new logo was designed in 2011, 50 years after the first Auchan store opened its doors. Illustrating the wealth of talent within the company, the logo was designed by a member of the group's staff and selected from among 750 proposals made by teams and staff in all the countries where Auchan operates.
The three silhouettes symbolize the company's core values of trust, sharing and progress.
> €48.1 billion in consolidated revenue before tax in 2013
> €62.1 billion in revenue including taxes, for the chains in 2013
> A 2013 workforce of 302,500 employees performing more than 150 different jobs
> A presence in 16 countries
Auchan insists on making sustainable development a daily reality, both in the social and ecological environment of the stores and through greener patterns of consumption.


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Lipscomb's efforts have helped his clients recoup some of their losses, but they haven't earned him any friends online. "Someone once told me: 'Go sodomize yourself with a baseball bat,'" he says. But whereas the young attorney takes such abuse as a sign that he's doing his job, others say he's tormenting innocent Americans.
"Lipscomb really scares people with these pure bills of discovery," says the founder of website fightcopyrighttrolls.com, who declines to give his name. He concedes Lipscomb is "an extremely smart guy" but says he is notorious for letting porn companies or their hired guns — called "trolls" because they mindlessly scour the Internet — hustle people for settlements.
Several posts on the website refer to Lipscomb. Others rail against the broader practice of punishing porn downloaders. "Trolls are trying to get us for infringing on a copyright, but they are also trying to shame us. Shame us for what? After all, everyone watches porn," argues one anonymous commenter. "But what are the trolls doing?... "[They] are dirtier than we are."
The simmering anger against Lipscomb and his tactics boiled over this past July. That's when Barker filed a class-action lawsuit against five of Lipscomb's clients — including Malibu Media and Patrick Collins — claiming harassment and fraud. Lipscomb says that her suit is "frivolous" and that Henry has "no clue what he's doing."
"There is nothing unfair orunrighteous about holding a thief to account," Lipscomb says.
But Barker says she isn't a thief, and she isn't backing down. "I am standing up for my rights not to be bullied," she says. "If it is happening to me, it is happening to other innocent people. Whether it is a porn company or Disney, I won't give them money for something I didn't do."
@TamaroffLaw @fightcopytrolls @DieTrollDie @EFF Name of that tune, lesson learned... don't get on the internet with your IP exposed!
Some of he folks in the porn industry apparently aren't above a little blackmail to boost the bottom-line. Who'd guessed?
@TamaroffLaw @fightcopytrolls @EFF Love the pic the author used for the article! :)
Tamaroff & Tamaroff, P.A. fights copyright trolls - http://tamarofflaw.com/florida-copyright-troll-defense/
what the point of making news porn movies? everybody know to ending
With porn movies, when you're watching them, it's basically they're an hour and a half long. And who watches an entire funcking porn movie?


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ACEhp has made exciting changes to our website! Our new website platform requires that all members reset their passwords before logging into the new site the first time. Please click here to request a temporary password and access your account and reset your password. Learn more about the new site and what is ahead for ACEhp in 2015. Email our support team if you need further assistance. |
The Alliance is a community of professionals dedicated to accelerating excellence in health care performance through education, advocacy, and collaboration. Join ACEhp and gain immediate access to tools, best practices and experts to take your career to the next level. |
What's New at the AllianceLearn more about the Alliance's improved website Now Available - The QIE roadmap The Quality Improvement Education (QIE) Roadmap: A Pathway to Our Future Alliance Hosts What Continuing Educators Need to Know About IPE - Archive Recording & Presentation Alliance Hosts December Town Hall Webinar - Archive Recording |
Upcoming Events |


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Shueisha Edition Release Date: November 4, 2004
Proper Japanese Title: Tatakau Shikaku (闘う資格)
Fearing that she's no match for the deadly Claymore named Ophelia, Clare spirits her companion Raki away to relative safety. But no matter how far they run, they won't be able to escape Ophelia's heightened senses. And now that Ophelia knows that Clare has awakened, she will not rest until she has destroyed Clare.
The one armed Clare is taught a new style of swordsmanship by Irene that she must use against the awakened Ophelia.
Url: | |
BBCode: | |
Added by: | pikahyper |
Date Added: | Feb. 24, 2013 |


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Cesena 1-0 Udinese
A strike from substitute Alejandro Rodriguez helped relegation-threatened Cesena claim a 1-0 victory over Udinese.
The Spanish forward headed home the winner in the 76th minute to hand the hosts just their fourth win of the campaign.
The result allowed Cesena to close the gap on Atalanta, who hold the last position of safety, to four points while Udinese dropped to 13th place after suffering a third successive defeat.
The Friuli outfit have not won since January 26 but went into the contest at the Dino...


text: MPN: TL546APW EAN: 5023790027960 The Beko TL546APW tall fridge gives you plenty of storage space for your week's shopping without taking up much floor space in your kitchen.The spacious 256-litre interior includes 5 glass shelves, a 4-bottle chrome wine rack, salad drawer and 4 door compartments, making it easy to find what you're looking for quickly. An A+ energy rating saves you money by keeping your bills to a minimum, while taking care of the environment. A reversible door lets you fit this Beko tall fridge in any corner of your kitchen. This Beko tall fridge is auto defrost, which keeps maintenance to a minimum. Useful info:


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When a Man Loves a Woman (1994) 6.5
An airline pilot and his wife are forced to face the consequences of her alcoholism when her addictions threaten her life and their daughter's safety. While the woman enters detox, her husband must face the truth of his enabling behavior. Director:Luis Mandoki |
Watch Trailer 0Share... |


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New Products
The former ``Hard Hat'' gets a friendly open-source integrated development environment, along with more hardware support, including 802.11b.
MontaVista has removed Hard Hat from its name and added the KDevelop integrated development environment (IDE) to its 2.1 release. KDevelop offers a choice of user interface look-and-feel modes, including one that is similar to Microsoft Visual C++. Other changes in the 2.1 release include: 802.11b support; two journaling filesystems, ext3 and reiserfs; support for more hosts including PowerPC Linux; and support for kernel preemption on SMP targets. Microsoft Windows, via VMware, and several Linux distributions running on both x86 and PowerPC are supported hosts. MontaVista Linux 2.1 also targets more than 20 processors from six architectures, including x86/IA-32, PowerPC, StrongARM, XScale, ARM, MIPS and SH. These CPUs constitute more than 60 boards, including 18 new boards, supported by the 2.1 release.
MontaVista Linux 2.1 also offers enhanced customer documentation that follows a typical development cycle. Individual and customized Quick Start Guides for each supported board are also included in this version.
KDevelop is developed by an international group of programmers and licensed under the GNU GPL. MontaVista has contributed some bug fixes and some support for cross-compilation to the KDevelop team. Although MontaVista's contributions are available from individual project sites, the freely downloadable Journeyman edition of the complete distribution will be gone with the 2.1 release and replaced by a demo kit.
Manufacturer: MontaVista Software
Model: Version 2.1
Suggested Retail Price: Contact manufacturer
The TS100 has dual 32-bit CPU architecture, a PowerPC CPU for applications and a dedicated RISC CPU for I/O.
The Cyclades-TS100 Device Server is a high-end, high-performance, single-port device server for control and automation applications. It offers extensive flexibility and support for customizations and additional functionality incorporated by the end user or system integrator. The TS100 connects serial devices to the TCP/IP network and can be used in industrial automation, out-of-band network management and retail automation, or it can be used to integrate legacy devices into the network.
One Ethernet 10/100BT LAN connection and one RS-232/RS-485, software-selectable, are supported. The TS100 features 4MB Flash memory and 16 MB SDRAM (expandable to 32MB), and security features include SSHv2, socket authentication and packet filtering. Management features are text-based on web interfaces, and a CDK for custom applications is provided. The TS100's dimensions are 2.76" × 3.35" × 1.18".
Manufacturer: Cyclades Corporation
Model: Cyclades-TS100
Suggested Retail Price: $389 US
RidgeRun and Texas Instruments deliver system-level DSPs for embedded and connected applications.
RidgeRun and Texas Instruments (TI) combined efforts on an out-of-the-box development environment for building real-time applications. Based on RidgeRun's DSPLinux OS and board support package (BSP) and TI's programmable, system-level digital signal processors (DSPs), the suite will reduce cost, power consumption and board space for designers of real-time embedded applications. The suite can be used by developers of applications ranging from wireless data, smart pen pads, voice recognition, network security and industrial control. The suite also allows access to real-time algorithms running on code-compatible TI DSPs from the kernel.
RidgeRun's contributions to the suite include command-line build tools that support TI's DSPs as well as the ARM7 Thumb RISC processor, a JTAG-based GDB debugger and a kernel with DSP/BIOS bridge support for C54x DSP targets. Over 1,000 existing middleware software packages, communication stacks and other applications available for the ARM7 are also included. Overall, the development suite enables native DSP-enabled performance for low-cost RISC-based systems.
Manufacturer: RidgeRun, Inc. and Texas Instruments
Model: Embedded Linux Development Suite, various bundles
Suggested Retail Price: Depends on bundle
As Linux continues to play an ever increasing role in corporate data centers and institutions, ensuring the integrity and protection of these systems must be a priority. With 60% of the world's websites and an increasing share of organization's mission-critical workloads running on Linux, failing to stop malware and other advanced threats on Linux can increasingly impact an organization's reputation and bottom line.
Sponsored by Bit9
Most companies incorporate backup procedures for critical data, which can be restored quickly if a loss occurs. However, fewer companies are prepared for catastrophic system failures, in which they lose all data, the entire operating system, applications, settings, patches and more, reducing their system(s) to "bare metal." After all, before data can be restored to a system, there must be a system to restore it to.
In this one hour webinar, learn how to enhance your existing backup strategies for better disaster recovery preparedness using Storix System Backup Administrator (SBAdmin), a highly flexible bare-metal recovery solution for UNIX and Linux systems.
Sponsored by Storix
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- Resurrecting the Armadillo
- High-Availability Storage with HA-LVM
- March 2015 Issue of Linux Journal: System Administration
- Real-Time Rogue Wireless Access Point Detection with the Raspberry Pi
- DNSMasq, the Pint-Sized Super Dæmon!
- Localhost DNS Cache
- Days Between Dates: the Counting
- The Usability of GNOME
- Linux for Astronomers
- You're the Boss with UBOS


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Spanish league club Rayo Vallecano are offering discounted tickets to unemployed fans for Sunday's game against Real Madrid in a bid to help club members affected by Spain's financial crisis.
Rayo says it will make 1 000 tickets available at the price of €5 to unemployed supporters for the crosstown derby with the Spanish leaders.
Ticket prices for regular members remain between €20 to €25 as the club hopes to fill their Vallecas Stadium.
Debt-ridden Spain has Europe's highest unemployment rate at nearly 23 percent, including a jobless rate of 39 percent for those aged between 20 and 29.
Rayo have won three straight games to sit eighth in the standings.


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Well, what do we expect when Sex is the main interest in selling products, designing clothes for younger kids to wear, music, games, television shows are innundated with how to appeal sexually to everyone. Our academic establishments are not paying attention and neither are the parents.
Way back during Clinton's Impeachment the shocking actions of our kids were exposed by teachers, bus drivers and the kids themselves discussing how common oral sex was on the way to school, during school classes and during recess. What the hell more do we need to do before we take charge of the board of education and the school administrators?
Peterson's actions were known before this final action that she took with her student. The parents knew when they asked the school to take action. Apathy and an oversexed student body will only be removed when the parents take charge of their kids and dump the damn television, video games and the crap their kids learn in the classroom.
Where is the outrage that could have kept this woman away from her students?


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Earlex Spray Station HV5500
Get a pro finish with in a lightweight protable package. Buy Now & Save $40
WoodRiver® Adjustable Base $59.99
Small shop? You need to put your tools on wheels. Buy Today & Save $25
7-Piece Forstner Bit Set $16.25
No shop should be without a great set of forstner bits. Save 50%!
Look up product instructions, videos and articles about your favorite tools using our Learning Center.
Join us on your favorite social network! Share your projects and learn from other woodworkers.
Please Note: This item can only be shipped ground to the 48 contiguous states. Some items cannot be shipped via air due to being flammable, magnetic, contains batteries, hazardous fumes or where contents are under pressure. Please use a physical address not a PO Box during checkout.
(1 reviews)
Product Information:
Ideal for dressers and nightstands, the curving lines of these bun feet will add simple elegance to your project.
Hanger bolt not included.
Hardwood recommended for paint grade only.
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This product ships direct from the manufacturer:
If you have a return issue, contact Woodcraft Supply customer service @ 1-800-535-4482 for return details. Returns are subject to a re-stocking charge of 20% of the list price of the item returned and require pre-approval. All returns must be in original, unused condition. Customer is responsible for return shipping.
When returning product, please include the invoice number or customer number and refer to the back of the invoice for shipping instructions.
If purchased at a Woodcraft Store, please return the item directly to the store where your purchase was made.
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good design
By steve
from jacksonville, fla
About Me Avid Do-It-Yourselfer
Best Uses
Comments about Grand River Corner Pedestal Bun Foot - Hardwood, Model BFPED-C-H :
lack of describing what spieces of wood, does it accept staining, etc...


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The Magnus VC-20-SCP Zoom Controller for Canon, Panasonic, Sony provides record and zoom control for all LANC-equipped Canon, Panasonic, and Sony camcorders. An included 22.5" long control cable connects into the controller on one end and into the camcorder's LANC port on the other. You can also use this controller with Sony consumer cameras via an optional Magnus ACM-SAVR AVR adapter cable.
A built-in mounting clamp allows attaching the controller to your tripod pan handle so you can zoom and pan with one hand and achieve a fluid look. The clamp opens up to 1.25" and also fits jibs, tubes, pipes, and bars. Zoom is regulated by a pressure-sensitive rocker switch that produces faster or slower control depending on how hard or gentle you press it. For tape-based cameras, a REC/RET button works together with a REC/RET switch to let you return to the last record position.
Cable Port | 2.5 mm stereo cable jack |
Cable Length | 22.5" (57.2 cm) |
Clamp Size | Opens to a max of 1.25" (3.2 cm) |
Dimensions | 3.6 x 2.2 x 3.3" (9.1 x 5.6 x 8.3 cm) |
Weight | 4 oz (113.4 g) |


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Michigan State University College of Law
Legal Clinic
610 Abbot Rd.
East Lansing, MI 48823
517-336-8089 fax
MSU Law provides superior academic and practical preparation through a number of experiences, including its clinical programs. While discovering your niche as a lawyer and gaining practical experience, you are part of the Law College's mission to provide service to the community and inspire students to use their legal education for the betterment of society.
The MSU Legal Clinic will hold an informational session for students interested in enrolling in one of the clinics for summer and fall. "Intro to Clinics" will be held on Thursday, March 5th from 12:15pm-1:15pm in room 473 at the law college. All students interested in enrolling should attend, and all clinics require an application.
The application for the summer is due on Friday, March 20th. Please fill out the application and email it, along with required documents, to Jesse Alvarez.
The application for the fall is due on Friday, April 10th. Please fill out the application and email it, along with required documents, to Jesse Alvarez.
What: Intro to Clinics
When: Thursday, March 5 from 12:15pm-1:15pm
Where: Room 473, Law College
Tax Clinic Receives Grant from IRS for 2014
The IRS has announced that the Tax Clinic is one of 133 low-income taxpayer clinics to receive a matching grant for 2014. The Tax Clinic will use the $98,000 grant, along with matching funds, to continue its free legal services to low-income taxpayers and to taxpayers for whom English is a second language. While this award is tremendous, the Tax Clinic must still rely on other sources of grants and charitable contributions to fund its annual costs. Please support the Tax Clinic and make a tax-deductible contribution. For more information about the grant, see the IRS announcement.
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Love this jacket! Wore it to a business meeting and I swear it brought me luck. I call it my good luck jacket. It looks expensive and the fit ins perfect and true to size. The color is a true silver so it looks good with brown or black. I wish I could find the pants that match the jacket.
This jacket is a show-stopper for work and after hours. I bought it to go with a silver textured skirt. It went perfectly, as the colors contrasted. There is not a lot of stretch to this jacket, but that gives it a more polished look. The sizing was spot-on. The picture does not to this jacket justice. It is a nice piece to have. The price was right and I received it quickly. I am looking for the next good deal.
This is a very professional gray/silver jacket. Normally I'm a 12, but since I have broad shoulders, I ordered a 14, and it fit perfectly. The shimmer is not over the top. I haven't worn it yet to see how it looks at the end of the day, but it seems like it'll be fine.
Items must be returned in new or unused condition and contain all original materials included with the shipment. More details
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* For your protection, all orders are screened for security purposes. If your order is selected for review, our Loss Prevention Team may contact you by phone or email. There may be a two business day delay to process your order.
** Most Oversize orders are delivered within 1-4 weeks. Some orders may take 6 weeks to be delivered.


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Junior Member
Today i just had a game with a kass i was for sure we were gonna win we were winning top and bot lane and then Kass afks and we lost two towering and got ganked...
lol brutal...
Man Bear Pig Hoe
yer just things like that and it wouldnt get abused because it is the enemy team so why would they want to allow u to save elo unless you really deserve it
Senior Member
No. Wins or nothing. There is no elo hell, there are barely any trolls, and people don't understand how elo works. If you're good enough to be a gold player, you'll be in gold. If not, then you won't be. Simple as that. You can't determine how well somebody played based on their end score. You could lose spectacularly in the laning phase and get carried back into contention.
i got my elo back so meh im happy
King Kappa
If you're really good, you carry the feeders to higher Elo.
ELO is not luck since everything balances out, i bet you get a lot of free wins because people afk/feeding on the other team. All you have to do is keep playing games, be positive, tell your team where/what to do if you think you should be in a higher ELO telling people what to do is one of the best ways to gain elo. I'm not high elo at all im just saying this from what I've seen. i lost most of my placement matches so i was down at 800 elo ish then decided i wanted to get better and started to carry myself up to 1400. TL'DR always be positive, dont rage, tell team what to do, play to win.
I voted maybe. You got a very... aggressive attitude. No one is going to refund your ELO because of trolls. It happens to everyone else, even at 2100 ELO. Trolls exist everywhere and they do what they do to make umadbro.
NME Potato Zero
ITT: herp derp i get 10 cs more than my laner i deserve to be platinum herp elo hell derp


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Satechi Smart TV Box review: Android-based set-top box does a lot, but doesn't make it easy
It can handle nearly anything your phone can do, but the learning curve is long.
HBO Go arrives on Amazon's Fire TV streaming box
Service offerings for Amazon's set-top box catches up with Apple TV, Chromecast, and Roku, but the Amazon Fire TV Stick won't get it until the spring.
Review: Simple.TV 2 knows some great DVR tricks but also has flaws
This network DVR costs less to run than a TiVo Roamio OTA, but we had a few issues with its lack of Wi-Fi and its excessive fan noise.
What a cable-free HBO subscription could mean for Apple TV
HBO Go might just be the first domino to fall.
Five things Apple should fix in the next Apple TV update
Family Sharing and Beats Music are nice and all, but Apple needs to go a lot further to make Apple TV as easy to use as Roku and Fire TV.
SlingTV review: Convenient TV place-shifting, with an underwhelming new interface
For the first time, SlingTV puts an informational overlay on your TV screen, to help you find what to watch. We're just not sure it's worth the upcharge from the capable Slingbox M1.
11 dorm room gadgets that are too cool for school
You're going to spend the next nine months on campus. We've got suggestions for the gear that can turn that spartan dorm room into a home away from home.
Your complete guide to every Apple TV channel, A to Z
We break down all the Apple TV apps: what you'll find there, how much you'll pay for a subscription, and which cable provider logins are supported.
20 essential Roku channels for dedicated couch potatoes
Roku boasts more than 1500 channels, but that's overkill by far. Install these 20 and you will never, ever run out of stuff to watch.
More content for the Fire TV, from sources other than Amazon
Four months after its launch, Amazon's set-top box gets new channels from Disney, MLB, Animal Planet, and more.
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Free ModelSim PE Evaluation Software
Software Evaluation
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text: TOP 10 Top 10 Cars Top 10: Badass Movie Cars Back to Top ▲ Top 10: Badass Movie Cars 2 The Tumbler Share on Facebook Share on Twitter 0 Shares Warner Bros. Pictures Batman Begins Batmobiles have always been badass movie cars relative to their times, but just as Batman Begins was radically different from the other installments, so was the car. The Tumbler wasn't based on a single vehicle; instead, it seems to have drawn inspiration partly from Frank Miller's graphic-novel depiction in The Dark Night Returns and possibly also the F-117 Nighthawk stealth fighter jet. Whatever the case, it was done well and made Bruce Wayne's Lamborghini pale in comparison. Next Page TOP 10 9 8 7 6 5 4 3 2 1 Comments Show Comments More Like This Promoted Around the Web Special Features