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Screen protectors for eXo released
Lajo the designer and manufacturer of eXo announces Shades, a 1.5mm soft, scratch resistant molded TPU screen protector for the 3rd gen iPod is now available and will be shipped to all distributors and resellers this week from his factory in China. Shades totally covers the screen area and a 1.0mm wide rim fits nicely under the eXo skin. The combination of Shade
Belkin Battery Pack "Can" Charge iPod
The Belkin Battery Pack states that it will not charge the iPod, rather it will just provide power to the iPod when you plug it in directly. I've discovered that when you plug the battery pack into the dock, place the iPod on the dock, it will charge your iPod and allow it to function normally as if plugged into the adapter.
Editor's note: Please click 'Read more' for an official comment from Belkin on the above discovery.
Refurbished 3G iPods for sale at Apple Store
Deals on the Web: A reader points us to the Apple Store online for factory refurbished 3rd generation iPods in three sizes. The 10GB iPod is $249, the 15GB iPod is $329 and the 30GB iPod is $429. With Free ground shipping, these are among the lowest prices we've seen. Click the "Specials Deals" link at the bottom of the page to find the deal.
BusinessWeek: Steve Jobs: "I'm an Optimist"
BusinessWeek online has posted an interview with Apple CEO Steve Jobs. "Apple's visionary-in-chief looks at tech's condition today and says: "I see a recovery in innovation coming out of this downturn"
Q: Will we see more groundbreakers like the iPod from Apple?
A: The iPod is not a new category. Music is not new. It's not a speculative market. It's a very, very large market. It's been around for thousands of years and will be around as long as humans exist. So it's not like saying we're going to go build an information appliance or some technical curio and hope the market exists. We're taking a giant market and bringing it into the Digital Age. That requires hardware, software, and the ease-of-use talent that Apple has.
Best Price on 15GB iPod at CompUSA
I just purchased a 15GB iPod from CompUSA yesterday for $350 in Pittsburgh, PA. It is a non-advertised promotion for this week - just ask the Customer Service Assistant.
Pre-Order XtremeMac Deluxe for 3G iPods
XtremeMac has updated their website with information and photos about its new Deluxe iPod case for 3G iPods. The case has a patent pending design feature where the iPod is held in place without anything in the front covering the face of iPod. It also features a large access hole for the headphone/remote ports, and the front flap with Velcro closure can be opened without having to unplug the cable. The Deluxe case comes in Black Leather or Camel Tweed and is available for pre-order at $29.95 (includes belt clip). We asked XtremeMac when the case would ship and the reply was, "Most likely in 2 weeks, but I should know an exact date by tomorrow." We'll post an update if and when the ship date is confirmed.
10% off iPods at Target.com
Dealmac: Target.com sells Apple's iPod MP3 players, as a reader found. Several 10% off coupons are available at Target.com, resulting in the lowest available prices on current-generation iPods. The iPod 15GB costs $359.99 after discount, while the iPod 30GB costs $449.99 after discount.
Shipping adds about $7, and sales tax may reduces most of the savings from the coupon.
Amazon: 15 and 30GB iPods at reduced prices
Amazon.com has reduced prices on iPods; 30GB iPod for $474.05 and the 15GB iPod for $379.05. Just add to cart and the reduced price shows up. Not the cheapest price seen but better than the usual $499/$399.
iPod 15GB for $370 and $380
Dealmac: Although we've seen much lower prices, readers recommend two ways to get the Apple iPod 15GB MP3 player for less than its $400 list price. Dell Home sells the iPod 15GB for $399.99. Add anything at all to your cart and apply coupon code "32F3C9A87986" for a $369.99 price (not including the additional item). Or, BestBuy.com sells the iPod 15GB for $379.99. Either way, shipping is free. Dell's coupon ends today.
Philadelphia Inquirer: Industry could embrace, not fight, technology
"That's only a beginning. Below are a few ideas that move in the same direction. Born in a rash moment of gadget dreaming, some are wildly impractical, and others might be reality already.[...]
iPod groovebox. Your basic drum machine is a box with pads on it that trigger different sounds: You push pad A and get a booming kick drum, pad B gives you a cymbal, and so on. The iPod MP3 player comes with a rabbit's-foot-size remote control device that has four buttons on it, controlling Play, Stop, Fast Forward and Rewind. What would happen if those four buttons triggered sounds instead of transport controls?"
iCreate magazine issues a complete guide to iPod
In Issue 2 of iCreate Magazine, Paul Newman investigates how Apple's iPod has turned the heads of music fans across the globe, but there's far more to this digital music player than meets the eye.
iLounge also gets picked as the number 2 web resource after Apple iPod Support - "Without doubt the most stylish iPod site on the Net, iLounge is brimming over with news, views, information on outrageous accessories and tips for better iPod usage. Make sure you register with the site and log on to the forums for the full experience."
iCreate included a mock photo of an iPodDV device. Imagine a 3G iPod on its side, touch wheel on the right, buttons across the top of touchwheel and a large LCD display on the left, and an iSight camera attached to the left. "Record video, photographs music - 120 gb of your life, in your pocket. Say cheese" The magazine also includes a CD with an iPod Toolkit with such utilities as iPod Access 1.3, iPodViewer 2.0.1 and RIP2POD 1.0.
iPod Software 2.0.2 installed on some iPods
Apple has posted a KnowledgeBase Document with information about iPod Software 2.0.2 installed on some iPods:
"Some iPod (with Dock Connector) models include iPod software 2.0.2, which is an update used in manufacturing and has no functional changes.
Certain iPod (with Dock Connector) models include iPod software 2.0.2. The 2.0.2 version of the iPod software is an update used in manufacturing and contains no functional changes.
It is not required, and is not available as a download."
MacMice updates GPerch iPod stand
MacMice has released an updated version to its GPerch all aluminum iPod stand. The GPerch stand mimics the design of the yet-to-be-released Apple G5 Mac and now sells for $29.99 (originally $39.99)
All clear FlipStand now shipping
Speck Products has announced that their new, all clear FlipStand is now shipping. The new 3G iPod case features a custom dock (works with existing iPod cable that comes with all iPods), doubles as a stand, access to scroll wheel, clear window LCD protection, detachable belt clip, and custom inserts for 10, 15, & 30GB models. The new FlipStand sells for $29.95.
Attention all business owners: Buy iPods for your employees
The Apple Store for Business is now offering an iPod Volume Discount Program. The program states, "iPods custom engraved with your company logo or message. Only available through the Apple Store for Business." You can call Apple for details and pricing.
Using a USB 1.1 Connection with 3G iPod
AppleCare KnowledgeBase Document:
"Some Windows-compatible computers may be able to connect to iPod (with Dock Connector) using a USB 1.1 connection, but this is not a recommended configuration. [...]
You may be able to connect an iPod (with Dock Connector) model to a Windows-compatible computer using a USB 1.1 connection, but this is not recommended because the connection is too slow to synchronize music. For best performance, connect iPod to your computer using USB 2.0 or FireWire. You may need to purchase and install a USB 2.0 or FireWire card to do this."
CNET: Ultimate iPod accessories
CNET recently posted a list of 'Ultimate iPod accessories.' We were familiar with all but one of the items on the list, the Audi-Oh… a "superaccurate massager that connects to your iPod and vibrates to the beat of your music." It's not yet available online, but if you're interested give the company a call.
New 30GB iPod: $449.99
Deals on the Web: Target has restocked the new 30GB iPod for $499.99. Use coupon code "TCAF2SAVETEN" during checkout to save 10% for a final price of $449.99. It's the best current price we've seen on a new unit.
How BuyMusic really makes their money
BuyMusic's ads are served by the infamous DoubleClick network. In the past, Buy.com's profit philosophy was to underprice products and make up for the loss in profits by advertising, and it's very likely BuyMusic.com is doing the same thing. The site's privacy policy says that BuyMusic.com itself will not collect IP addresses and other personal information. But that doesn't keep them from doing so in a roundabout way:
"We use a third party to provide digital music download samples for our customers. That service collects your IP address, browser type, ISP, platform, and a date/time stamp."
The excerpt means that "third party" will sell your info (like the songs you've listed to) to many other third parties. In essence, you buy one James Taylor CD, you'll see James Taylor merchandise everywhere you go on the internet.
iPod Armor case for $40
Big discounts on the iPod Armor Case at DirectCase.com. The Armor for the original iPods was $54.95. It is available now for $40.00.


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Publications (16)62.96 Total impact
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ABSTRACT: The histidine kinase, CheA, couples environmental stimuli to changes in bacterial swimming behavior, converting a sensory signal to a chemical signal in the cytosol via autophosphorylation. The kinase activity is regulated in the platform of chemotaxis signaling complexes formed by CheW, chemoreceptors, and the regulatory domain of CheA. Our previous computational and mutational studies have revealed that two interdomain linkers play important roles in CheA's enzymatic activity. Of the two linkers, one that connects the dimerization and ATP-binding domains is essential for both basal autophosphorylation and activation of the kinase. However, the mechanistic role of this linker remains unclear, given that it is far from the autophosphorylation reaction center (the ATP binding site). Here we investigate how this interdomain linker is coupled to CheA's enzymatic activity. Using modern nuclear magnetic resonance techniques, we find that by interacting with the catalytic domain, the interdomain linker initiates long-range structural and dynamic changes directed towards the catalytic center of the autophosphorylation reaction. Subsequent biochemical assays define the functional relevance of these NMR based observations. These findings extend our understanding of the chemotaxis signal transduction pathway.Biochemistry 01/2014; · 3.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Bacterial chemotaxis is one of the best studied signal transduction pathways. CheW is a scaffold protein that mediates the association of the chemoreceptors and the CheA kinase in a ternary signaling complex. The effects of replacing conserved Arg62 of CheW with other residues suggested that the scaffold protein plays a more complex role than simply binding its partner proteins. Although R62A CheW had essentially the same affinity for chemoreceptors and CheA, cells expressing the mutant protein are impaired in chemotaxis. Using a combination of molecular dynamics simulations (MD), NMR spectroscopy, and circular dichroism (CD), we addressed the role of Arg62. Here we show that Arg62 forms a salt bridge with another highly conserved residue, Glu38. Although this interaction is unimportant for overall protein stability, it is essential to maintain the correct alignment of the chemoreceptor and kinase binding sites of CheW. Computational and experimental data suggest that the role of the salt bridge in maintaining the alignment of the two partner binding sites is fundamental to the function of the signaling complex but not to its assembly. We conclude that a key feature of CheW is to maintain the specific geometry between the two interaction sites required for its function as a scaffold.PLoS Computational Biology 11/2013; 9(11):e1003337. · 4.83 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A quantitative understanding of how conformational transitions contribute to enzyme catalysis and specificity remains a fundamental challenge. A suite of biophysical approaches was used to reveal several transient states of the enzyme-substrate complexes of the model DNA cytosine methyltransferase M.HhaI. Multidimensional, transverse relaxation-optimized nuclear magnetic resonance (NMR) experiments show that M.HhaI has the same conformation with noncognate and cognate DNA sequences. The high-affinity cognatelike mode requires the formation of a subset of protein-DNA interactions that drive the flipping of the target base from the helix to the active site. Noncognate substrates lacking these interactions undergo slow base flipping, and fluorescence tracking of the catalytic loop corroborates the NMR evidence of a loose, nonspecific binding mode prior to base flipping and subsequent closure of the catalytic loop. This slow flipping transition defines the rate-limiting step for the methylation of noncognate sequences. Additionally, we present spectroscopic evidence of an intermediate along the base flipping pathway that has been predicted but never previously observed. These findings provide important details of how conformational rearrangements are used to balance specificity with catalytic efficiency.Biochemistry 02/2013; · 3.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The binding of the soluble cytoplasmic protein FliG to the transmembrane protein FliF is one of the first interactions in the assembly of the bacterial flagellum. Once established, this interaction is integral in keeping the flagellar cytoplasmic ring, responsible for both transmission of torque and control of the rotational direction of the flagellum, anchored to the central transmembrane ring on which the flagellum is assembled. Here we isolate and characterize the interaction between the N-terminal domain of Thermotoga maritima FliG (FliG(N)) and peptides corresponding to the conserved C-terminal portion of T. maritima FliF. Using nuclear magnetic resonance (NMR) and other techniques, we show that the last ~40 amino acids of FliF (FliF(C)) interact strongly (upper bound K(d) in the low nanomolar range) with FliG(N). The formation of this complex causes extensive conformational changes in FliG(N). We find that T. maritima FliG(N) is homodimeric in the absence of the FliF(C) peptide but forms a heterodimeric complex with the peptide, and we show that this same change in oligomeric state occurs in full-length T. maritima FliG, as well. We relate previously observed phenotypic effects of FliF(C) mutations to our direct observation of binding. Lastly, on the basis of NMR data, we propose that the primary interaction site for FliF(C) is located on a conserved hydrophobic patch centered along helix 1 of FliG(N). These results provide new detailed information about the bacterial flagellar motor and support efforts to understand the cytoplasmic ring's precise molecular structure and mechanism of rotational switching.Biochemistry 06/2012; 51(25):5052-60. · 3.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: In the bacterial chemotaxis two-component signaling system, the histidine-containing phosphotransfer domain (the "P1" domain) of CheA receives a phosphoryl group from the catalytic domain (P4) of CheA and transfers it to the cognate response regulator (RR) CheY, which is docked by the P2 domain of CheA. Phosphorylated CheY then diffuses into the cytoplasm and interacts with the FliM moiety of the flagellar motors, thereby modulating the direction of flagellar rotation. Structures of various histidine phosphotransfer domains (HPt) complexed with their cognate RR domains have been reported. Unlike the Escherichia coli chemotaxis system, however, these systems lack the additional domains dedicated to binding to the response regulators, and the interaction of an HPt domain with an RR domain in the presence of such a domain has not been examined on a structural basis. In this study, we used modern nuclear magnetic resonance techniques to construct a model for the interaction of the E. coli CheA P1 domain (HPt) and CheY (RR) in the presence of the CheY-binding domain, P2. Our results indicate that the presence of P2 may lead to a slightly different relative orientation of the HPt and RR domains versus those seen in such complex structures previously reported.Biochemistry 04/2012; 51(18):3786-98. · 3.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The basic structural unit of the signaling complex in bacterial chemotaxis consists of the chemotaxis kinase CheA, the coupling protein CheW, and chemoreceptors. These complexes play an important role in regulating the kinase activity of CheA and in turn controlling the rotational bias of the flagellar motor. Although individual three-dimensional structures of CheA, CheW, and chemoreceptors have been determined, the interaction between chemoreceptor and CheW is still unclear. We used nuclear magnetic resonance to characterize the interaction modes of chemoreceptor and CheW from Thermotoga maritima. We find that chemoreceptor binding surface is located near the highly conserved tip region of the N-terminal helix of the receptor, whereas the binding interface of CheW is placed between the β-strand 8 of domain 1 and the β-strands 1 and 3 of domain 2. The receptor-CheW complex shares a similar binding interface to that found in the "trimer-of-dimers" oligomer interface seen in the crystal structure of cytoplasmic domains of chemoreceptors from Escherichia coli. Based on the association constants inferred from fast exchange chemical shifts associated with receptor-CheW titrations, we estimate that CheW binds about four times tighter to its first binding site of the receptor dimer than to its second binding site. This apparent anticooperativity in binding may reflect the close proximity of the two CheW binding surfaces near the receptor tip or further, complicating the events at this highly conserved region of the receptor. This work describes the first direct observation of the interaction between chemoreceptor and CheW.Journal of Molecular Biology 12/2011; 415(4):759-67. · 3.91 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The bacterial histidine autokinase CheA contains a histidine phosphotransfer (Hpt) domain that accepts a phosphate from the catalytic domain and donates the phosphate to either target response regulator protein, CheY or CheB. The Hpt domain forms a helix-bundle structure with a conserved four-helix bundle motif and a variable fifth helix. Observation of two nearly equally populated conformations in the crystal structure of a Hpt domain fragment of CheA from Thermotoga maritima containing only the first four helices suggests more mobility in a tightly packed helix bundle structure than previously thought. In order to examine how the structures of Hpt domain homologs may differ from each other particularly in the conformation of the last helix, and whether an alternative conformation exists in the intact Hpt domain in solution, we have solved a high-resolution, solution structure of the CheA Hpt from T. maritima and characterized the backbone dynamics of this protein. The structure contains a four-helix bundle characteristic of histidine phosphotransfer domains. The position and orientation of the fifth helix resembles those in known Hpt domain crystal and solution structures in other histidine kinases. The alternative conformation that was reported in the crystal structure of the CheA Hpt from T. maritima missing the fifth helix is not detected in the solution structure, suggesting a role for the fifth helix in providing stabilizing forces to the overall structure.Journal of Biomolecular NMR 09/2011; 51(1-2):49-55. · 3.31 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The uropathogenic Escherichia coli colonize the host body by attaching themselves to the epithelial cells through the pyelonephritis-associated pili (pap). The expression of the papBA operon is regulated under a reversible phase-variation mechanism, which partitions the population of cells into those that express the pap and others that do not. The two phases of pap expression are the direct consequences of the two distinct DNA-binding modes exhibited by leucine-responsive regulatory protein (Lrp) in the pap promoter region. In the phase-OFF cells, Lrp occupies the binding sites proximal to the transcription start, blocking transcription initiation. In the phase-ON cells, Lrp occupies the binding sites distal to the transcription start and is thought to promote the CAP (catabolite gene activation protein)-directed transcription initiation. Lrp binds to the proximal binding sites more tightly than to the distal sites, and the switching from phase-OFF to phase-ON requires a local co-regulator, PapI. Here, we used PapI and an isolated DNA-binding domain construct of Lrp to show that there is a DNA co-recognition mechanism by which both proteins acquire enhanced affinity to the distal pap site DNA, to which neither of them binds to an appreciable extent without the other. Also, examination of the binding properties of the Lrp DNA-binding domain presented here led us to propose a new sequence alignment of the six pap Lrp-binding sites. New insights into the design of sequences regulating the pap phase variation as revealed by the pap Lrp-binding site sequences are thus defined and discussed.Journal of Molecular Biology 02/2011; 409(3):311-32. · 3.91 Impact Factor -
Article: No difference in kinetics of tau or histone phosphorylation by CDK5/p25 versus CDK5/p35 in vitro.
[Show abstract] [Hide abstract]
ABSTRACT: CDK5/p35 is a cyclin-dependent kinase essential for normal neuron function. Proteolysis of the p35 subunit in vivo results in CDK5/p25 that causes neurotoxicity associated with a number of neurodegenerative diseases. Whereas the mechanism by which conversion of p35 to p25 leads to toxicity is unknown, there is common belief that CDK5/p25 is catalytically hyperactive compared to CDK5/p35. Here, we have compared the steady-state kinetic parameters of CDK5/p35 and CDK5/p25 towards both histone H1, the best known substrate for both enzymes, and the microtubule-associated protein, tau, a physiological substrate whose in vivo phosphorylation is relevant to Alzheimer's disease. We show that the kinetics of both enzymes are the same towards either substrate in vitro. Furthermore, both enzymes display virtually identical kinetics towards individual phosphorylation sites in tau monitored by NMR. We conclude that conversion of p35 to p25 does not alter the catalytic efficiency of the CDK5 catalytic subunit by using histone H1 or tau as substrates, and that neurotoxicity associated with CDK5/p25 is unlikely attributable to CDK5 hyperactivation, as measured in vitro.Proceedings of the National Academy of Sciences 02/2010; 107(7):2884-9. · 9.81 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Enzymatic sequence-specific DNA modification involves multiple poorly understood intermediates. DNA methyltransferases like M.HhaI initially bind nonspecific DNA and then selectively bind and modify a unique sequence. High-resolution NMR was used to map conformational changes occurring in M.HhaI upon binding nonspecific DNA, a one base pair altered noncognate DNA sequence, and both hemimethylated and unmethylated cognate DNA sequences. Comparisons with previous NMR studies of the apoenzyme and enzyme-cofactor complex provide snapshots of the pathway to sequence-specific complex formation. Dramatic chemical shift perturbations reaching many distal sites within the protein are detected with cognate DNA, while much smaller changes are observed upon nonspecific and noncognate DNA binding. A cooperative rather than stepwise transition from a nonspecific to a cognate complex is revealed. Furthermore, switching from unmethylated to hemimethylated cognate DNA involves detectable protein conformational changes 20-30 A away from the methyl group, indicating high protein sensitivity and plasticity to DNA modification.Biochemistry 08/2009; 48(33):7807-16. · 3.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: CheA-short interacts with CheZ to localize CheZ to cell poles. The fifth helical region (residues 112 to 133) from the phosphotransfer domain of CheA interacts with CheZ and becomes ordered and helical, although it lacks a stable fold in the CheA fragment comprising residues 98 to 150 alone. One CheA molecule binds to one CheZ dimer.Journal of bacteriology 07/2009; 191(18):5842-4. · 2.69 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The high-resolution structures of nearly all the proteins that comprise the bacterial flagellar motor switch complex have been solved; yet a clear picture of the switching mechanism has not emerged. Here, we used NMR to characterize the interaction modes and solution properties of a number of these proteins, including several soluble fragments of the flagellar motor proteins FliM and FliG, and the response-regulator CheY. We find that activated CheY, the switch signal, binds to a previously unidentified region of FliM, adjacent to the FliM–FliM interface. We also find that activated CheY and FliG bind with mutual exclusivity to this site on FliM, because their respective binding surfaces partially overlap. These data support a model of CheY-driven motor switching wherein the binding of activated CheY to FliM displaces the carboxy-terminal domain of FliG (FliGC) from FliM, modulating the FliGC–MotA interaction, and causing the motor to switch rotational sense as required for chemotaxis.Journal of Molecular Biology 04/2009; · 3.91 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Alzheimer's disease (AD) is characterized by the intracellular accumulation of the neurofibrillary tangles comprised mainly of the microtubule-associated protein, tau. A critical aspect of understanding tangle formation is to understand the transition of soluble monomeric tau into mature fibrils by characterizing the structure of intermediates along the aggregation pathway. We have carried out multidimensional NMR studies on a C-terminal fragment of human tau (tau (187)) to gain structural insight into the aggregation process. To specifically monitor intermolecular interaction between tau molecules in solution, we combined (15)N- and (14)N-labeled tau, the latter of which was modified with a paramagnetic nitroxide spin label (MTSL). Paramagnetic relaxation enhancement (PRE) of (15)N-tau by interaction with MTSL- (14)N-tau allowed identification of low molecular weight oligomers of tau (187) that formed in response to heparin-induced aggregation. Two regions, VQIINK (280) and VQIVYK (311), were exclusively broadened by MTSL located at varied positions in the tau molecule. We propose that soluble oligomers of tau (187) are generated via intermolecular interactions at these motifs triggered by heparin addition. However, the associated line broadening at these motifs cannot be due to interaction between tau (187) and heparin directly. Instead, these specific interactions necessarily occur between tau molecules and are intermolecular in nature. Our data support the idea that VQIINK (280) and VQIVYK (311) are the major, if not sole, critical regions that directly mediate intermolecular contact between tau molecules during the early phases of aggregation.Biochemistry 08/2008; 47(28):7393-404. · 3.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimensional, transverse relaxation-optimized NMR experiments to assign nearly 80% of all residues in the cofactor-bound enzyme form, providing a basis for detailed structural and dynamical characterization. We examined details of the previously unknown effects of the cofactor binding with M.HhaI in solution. Addition of the cofactor results in numerous structural changes throughout the protein, including those decorating the cofactor binding site, and distal residues more than 30 A away. The active site loop is involved in motions both on a picosecond to nanosecond time scale and on a microsecond to millisecond time scale and is not significantly affected by cofactor binding except for a few N-terminal residues. The cofactor also affects residues near the DNA binding cleft, suggesting a role for the cofactor in regulating DNA interactions. The allosteric properties we observed appear to be closely related to the significant amount of dynamics and dynamical changes in response to ligand binding detected in the protein.Biochemistry 07/2007; 46(24):7261-8. · 3.19 Impact Factor -
Article: Solution structure of Escherichia coli PapI, a key regulator of the pap pili phase variation.
[Show abstract] [Hide abstract]
ABSTRACT: Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli to bind to epithelial cells and play an important role in urinary tract infection. Expression of pap is controlled by a phase-variation mechanism, based on the two distinct heritable states that are the result of adenine N6-methylation in either of the two GATC sequences in its regulatory region. The methylation status of these two sequences is sensed by the action of two proteins, Lrp and PapI, and they play a central role in determining pap gene expression in both phase-ON and phase-OFF cells. We used modern NMR techniques to determine the solution structure and backbone dynamics of PapI. We found its overall fold resembles closely that of the winged helix-turn-helix family of DNA-binding proteins. We determined that PapI possesses its own DNA-binding activity, albeit non-sequence-specific, independent of Lrp. PapI appears to bind to DNA with a K(d) in the 10 microM range. Possible mechanisms by which PapI might participate in the regulation of the pap operon are discussed in light of these new findings.Journal of Molecular Biology 02/2007; 365(4):1130-42. · 3.96 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Regulating the activity of the histidine autokinase CheA is a central step in bacterial chemotaxis. The CheA autophosphorylation reaction minimally involves two CheA domains, denoted P1 and P4. The kinase domain (P4) binds adenosine triphosphate (ATP) and orients the gamma phosphate for phosphotransfer to a reactive histidine on the phosphoacceptor domain (P1). Three-dimensional triple-resonance experiments allowed sequential assignments of backbone nuclei from P1 and P4 domains as well as the P4 assignments within a larger construct, P3P4, which includes the dimerization domain P3. We have used nuclear magnetic resonance chemical-shift-perturbation mapping to define the interaction of P1 and P3P4 from the hyperthermophile Thermotoga maritima. The observed chemical-shift changes in P1 upon binding suggest that the P1 domain is bound by interactions on the side opposite the histidine that is phosphorylated. The observed shifts in P3P4 upon P1 binding suggest that P1 is bound at a site distinct from the catalytic site on P4. These results argue that the P1 domain is not bound in a mode that leads to productive phosphate transfer from ATP at the catalytic site and imply the presence of multiple binding modes. The binding mode observed may be regulatory or it may reflect the binding mode needed for effective transfer of the histidyl phosphate of P1 to the substrate proteins CheY and CheB. In either case, this work describes the first direct observation of the interaction between P1 and P4 in CheA.Biochemistry 09/2006; 45(31):9509-17. · 3.19 Impact Factor
Publication Stats
159 | Citations | |
62.96 | Total Impact Points | |
Top Journals
University of California, Santa Barbara
- Department of Chemistry and Biochemistry
Santa Barbara, California, United States

subset: commoncrawl


text:
- Melissa Arnot
- Alex Barber
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- Katie Bono
- Adam Butterfield
- Anne Gilbert Chase
- Lance Colley
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- Leon Davis
- Elias de Andres Martos
- Pepper Dee
- Paul Edgren
- Mark Falender
- Leah Fisher
- Lindsay Fixmer
- Eric Frank
- Steve Gately
- Josh Gautreau
- JM Gorum
- Casey Grom
- Billy Haas
- Dave Hahn
- Walter Hailes
- Mike Haugen
- Bryan Hendrick
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- Joe Horiskey
- Nick Hunt
- Tyler Jones
- JJ Justman
- Levi Kepsel
- Andrew Kiefer
- Mike King
- Adam Knoff
- Caleb Ladue
- Ben Liken
- Zach Lovell
- Josh Maggard
- Paul Maier
- Linden Mallory
- Lindsay Mann
- Andres Marin
- Jeff Martin
- Stoney Molina
- Robert Montague
- Chase Nelson
- Billy Nugent
- Brent Okita
- Tyler Reid
- Kel Rossiter
- Geoff Schellens
- Shaun Sears
- Mike Soucy
- Garrett Stevens
- Jason Thompson
- Mark Tucker
- Mike Uchal
- Pete Van Deventer
- Alex Van Steen
- Ed Viesturs
- Christina von Mertens
- Mike Walter
- Seth Waterfall
- Solveig Waterfall
- Peter Whittaker
- Win Whittaker
- Bryson Williams
- Dan Windham
- Robby Young
Kilimanjaro: Waterfall & Team Summit!
Posted by: Seth Waterfall | July 30, 2013
Categories: *Expedition Dispatches *Kilimanjaro
Elevation: 19,340'
July 30, 2013 at 9:35 a.m. PST
Hi, this is Seth checking in from Mweka Camp. The summit was great today and I'll be emailing a pic out as soon as we get back to the hotel tomorrow. Everyone is tucked into their tents now listening to the pitter-patter of rain. We're hoping the morning brings some sunshine!
July 29, 2013 at 9:34 p.m. PST
Hey, this is Seth calling from Uhuru Peak, tallest point on Kilimanjaro, highest in Africa at 19,340'. The whole team made it, at least to Stella Point. We had a beautiful day with a few high clouds but zero wind for the whole climb and everyone did very well. We are going to take a few minutes, take some photos with the big sign and then head on back down. Tonight we will be dropping all the way down to 10,000 feet, which will feel great for us. Summit of Kilimanjaro for our team!
RMI Guide Seth Waterfall calls from the summit of Kilimanjaro!
On The Map
Sign Up For Kilimanjaro 2013 Email Alerts
665 views
What a way to celebrate your 40th birthday (with your Dad)! Miss you Don and Chris! lu
Posted by: Teri on 7/31/2013 at 9:21 am
Wonderful you all made it! So exciting—congratulations! Sounds like the weather was incredible making for some beautiful photos.
Posted by: Mary Beth Denefe on 7/31/2013 at 8:34 am


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come to WashingtonDC april 19 2010 to rally for you rights, for info go to second amendment march.com stand up for your gun rights
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Cemetery Inscriptions Collection
Association for Gravestone Studies Collection
Cemetery Inscriptions Collection, 1902-2005.
Call no.: MS 669
Founded in 1977, the Association for Gravestone Studies (AGS) is an international organization dedicated to furthering the study and preservation of gravestones. Based in Greenfield, Mass., the Association promotes the study of gravestones from historical and artistic perspectives. To raise public awareness about the significance of historic gravemarkers and the issues surrounding their preservation, the AGS sponsors conferences and workshops, publishes both a quarterly newsletter and annual journal, Markers, and has built an archive of collections documenting gravestones and the memorial industry.
Consisting of self-published and limited-run compilations of gravestone transcriptions from historical cemeteries, the AGS Cemetery Inscriptions Collection offers rich documentation of epitaphs and memorial language, with an emphasis on colonial and early national-era in New England and Ohio. The collection is arranged by state and town.
- Inscriptions
- Sepulchral monuments
- Association for Gravestone Studies
Flint and Lawrence Family
Flint and Lawrence Family Papers, 1642-1798.
Call no.: MS 273
Personal, financial and legal papers of Flint and Lawrence families of Lincoln, Massachusetts including wills, estate inventories, indenture documents, receipts of payment for slaves and education, correspondence; and records of town and church meetings, town petitions and receipts relating to the construction of the meeting house. Papers of Reverend William Lawrence include letter of acceptance of Lincoln, Massachusetts ministry, record of salary, a sermon and daybook. Personal papers of loyalist Dr. Joseph Adams, who fled to England in 1777, contain letters documenting conditions in England in the late 1700s and the legal and personal problems experienced by emigres and their families in the years following the Revolutionary War.
- American loyalists--Great Britain
- American loyalists--Massachusetts
- Church buildings--Massachusetts--Lincoln--Costs
- England--Emigration and immigration--18th century
- Flint family
- Immigrants--England--17th century
- Land tenure--Massachusetts--Lincoln
- Landowners--Massachusetts--Lincoln
- Lawrence family
- Lincoln (Mass.)--Economic conditions--18th century
- Lincoln (Mass.)--History
- Lincoln (Mass.)--Social conditions--18th century
- Massachusetts--Emigration and immigation--18th century
- Slaves--Prices--Massachusetts--Lincoln
- Adams, Joseph, 1749-1803
- Flint, Edward, 1685-1754
- Flint, Ephraim, b. 1714
- Flint, Love Adams, d. 1772
- Flint, Thomas, d. 1653
- Lawrence, William, 1723-1780
Types of material
- Accounts
- Genealogies
- Indentures
- Inventories of decedents estates
- Wills
Gale, Amory, 1800-1873
Amory Gale Ledgers, 1840-1872.
Call no.: MS 259 bd
A physician and native of Warwick, Mass., Amory Gale worked as an allopath after his graduation from Brown College in 1824, before turning to homeopathy in the mid-1850s. Often struggling with ill health, Gale plied his trade in a long succession of towns, including Canton, Scituate, Mansfield, and Medway, Massachusetts, as well as towns in Rhode Island and New Hampshire. Between 1844 and 1853, he interrupted his medical practice for a turn in the pulpit.
Gale's surviving ledgers include accounts with patients, their form of payment, lists of medical fees, and a draft of a business agreement with a fellow homeopath in Woonsocket, J.S. Nichols.
- Physicians--Massachusetts
Types of material
- Account books
Shapiro, Leon
Leon Shapiro Papers, 1939-1985.
Call no.: MS 127
Historian, author, Professor of Russian and Soviet Jewish History at Rutgers University, who helped arrange the escape of Jews from Europe during World War II and was active in several organizations concerned with the emigration of Soviet and Eastern European Jews to Palestine. Papers include biographical materials, correspondence, legal documents, writings, lecture and research materials, statistical data in the world Jewish population before and after World War II, oral history transcripts, pamphlets, newspaper clippings, and four photographs.
- Europe, Eastern--Ethnic relations--History--20th century
- Israel--Emigration and immigration--History
- Israel--History--1948-1967
- Jews, Soviet--History--Sources
- Jews--Europe, Eastern--History--Sources
- Jews--Migration--History
- Jews--Population--History
- Jews--Soviet Union--History--Sources
- Occupational training for Jews--History--Sources
- Palestine--History--1929-1948
- Romania--Emigration and immigration--History
- Rutgers University--Curricula
- Rutgers University--Faculty
- Soviet Union--Ethnic relations--History
- World ORT Union--History
- Shapiro, Leon
Types of material
- Oral histories
- Photographs
African American history
The acquisition of the papers of W.E.B. Du Bois in 1972 established SCUA as a center for research in African American history. In subsequent years, the University of Massachusetts has supported the publication of three volumes of Dr. Du Bois' correspondence and SCUA has served as a resource for many dozens of scholarly articles and books on Du Bois and his legacy. SCUA has also made efforts to build around the Du Bois collection, adding other important printed and manuscript materials both in African American history and in the history of efforts to promote social change.
Beyond Du Bois, significant collections in African American history include the papers of the abolitionist Hudson Family of Northampton, the expatriate playwright Gordon Heath, the sociologist, educator, and former president of Lincoln University, Horace Mann Bond.
Each February, in commemoration of Dr. Du Bois's birthday, SCUA and the Du Bois Department of Afro-Americans Studies at UMass co-sponsor a colloquium on Du Bois and his legacy. Our lecturers have included distinguished scholars such as Herbert Aptheker, Randolph Bromery, Clayborne Carson, and David Levering Lewis.
Significant collections
- Antislavery Pamphlet Collection
- Several hundred pamphlets relating to political antislavery in the United States, with an emphasis opn New England.
- See our Antislavery pamphlets digital archive
- Aronson, James. Collection, 1946-1983
- Editor of the National Guardian
- Banks, Katherine Bell. Papers, 1926-1960.
- Letters from W.E.B. Du Bois to Banks, a family friend
- Bond, Horace Mann. Papers, 1830-1979
- Educator, President of Lincoln University
- Brown, John. Papers (microfilm), 1826-1942 (bulk: 1856-1859)
- Du Bois, W. E. B. Papers, 1868-1963
- See also Du Bois Central for additional resources on Du Bois, including photographs, digital collections, and more.
See also the film documenting the Du Bois Homesite dedication ceremonies in Great Barrington, Mass., 1969.
- See also Du Bois Central for additional resources on Du Bois, including photographs, digital collections, and more.
- Heath, Gordon. Papers, 1940-1991
- Expatriate writer, actor, director, and musician
- Hudson Family. Papers, 1780-1955
- Family papers of the antislavery activist Erasmus D. Hudson
- International Oil Working Group. Records, 1981-1986
- Massachusetts. Special Commission on Unequal Education Opportunities. Records, 1974-1978
- Obrebski, Joseph and Tamara. Papers, 1923-1974
- Anthropological field work in Jamaica, 1947-1948
- Urban League of Springfield (Mass.). Records, 1970s
- Trent, Lloyd A. Family Papers, 1850-1996
Center for Community Access Television (Amherst, Mass.)
Center for Community Access Television Records, 1973-1989.
Call no.: MS 293
Group comprised of students from the University of Massachusetts and community members who sought to develop and promote cultural, literary, charitable, educational and public affairs television programming. Records include by-laws, articles of organization, organizational histories, annual reports, meeting minutes, correspondence, program schedules, subject files, brochures, handbills, news clippings, and materials relating to a proposed merger with University of Massachusetts Cable Vision. In 1989, CCATV was renamed Amherst Community Television (ACT).
- Amherst (Mass.)--Intellectual life--20th century
- Cable television--Massachusetts--Amherst--History
- Public-access television--Massachusetts--Amherst--History
- Television programs--Massachusetts--Amherst--History
- Center for Community Access Television (Amherst, Mass.)
Types of material
- Handbills
Girls Club of Greenfield (Mass.)
Girls Club of Greenfield Records, 1895-1995.
Call no.: MS 379
Founded in 1895, the Girls Club of Greenfield provides high quality early care and educational services to the girls of Franklin County, Massachusetts, and advocates for the rights of children and their families. During the school year, the Club offers diverse programming, ranging from an infant room and preschool to after school activities that promote teamwork, community spirit, social skills, and confidence. Since 1958, they have also operated a summer camp, Lion Knoll, in Leyden.
The records of the Girls Club of Greenfield include by-laws, annual reports, reports and meeting minutes of the Board of Directors, correspondence, and ledgers and account books. Also contains program files for daycare, summer camp, education worker programs, and others, personnel records, membership and committee lists, newsletters, press releases, ledgers, account books, scrapbooks, news clippings, photographs, slides, and artifacts.
- Girls--Massachusetts--Greenfield--Social conditions
- Girls--Massachusetts--Greenfield--Social life and customs
- Girls--Massachusetts--Greenfield--Societies and clubs--History
- Greenfield (Mass.)--Social conditions
- Greenfield (Mass.)--Social life and customs
- Girls Club of Greenfield (Greenfield, Mass.)
Types of material
- Account books
- Photographs
- Scrapbooks
Mission and history
To spark imagination and celebrate the legacy of innovation and the activist spirit, SCUA pursues an ambitious program of collecting materials of enduring historical value and offers strong support for research and learning. Through our collections, programs, public events, and exhibitions, SCUA promotes meaningful engagement with the record of social change in America and innovation and entrepreneurship, as well as the histories and cultures of the peoples of New England and the UMass Amherst community. We embrace the university's historic role as a center of knowledge for the people of the Commonwealth and are committed to using the highest professional standards and practices and the best available technologies to provide free and unfettered access to our holdings for all.
Department history
In 1931, nearly half a century after Librarian Henry Hill Goodell first authorized the permanent retention of the official records of Massachusetts Agricultural College, the Library established a College History Collection. Documenting the activities of the administration and faculty and the life of its students, this collection grew steadily, until in 1953, the Library dedicated a room named in honor of Dean William L. Machmer to serve as the first true home of the University Archives.
The timing of Goodell's proposal to preserve the College archives coincided roughly with the Library's first efforts to assemble a collection of rare books to support its educational mission. Although the campus had no separate library building until 1885, the College accepted several significant gifts of books, beginning as early as 1868, when the apiarist and state Adjutant General Henry K. Oliver donated twenty scarce volumes on bee culture, followed by other donations of important works in agriculture, history, and science. By the time the library published its first catalogue in 1875, rare books were a small, but distinctive part of the collections. Among the Library's earliest acquisitions were the first London edition of William Bartram's Travels Through North and South Carolina (1792), François Augier de Marigny's The History of the Arabians (London, 1758), and two early bee manuals by John Keys, The Practical Bee-Master (London, 1780) and The Antient Bee-Master's Farewell (London, 1796) — both courtesy of Oliver. All remain part of the collections today.
From its initial focus on agriculture, horticulture, and the natural sciences, the Library soon extended its collections to encompass the history and culture of New England. With the acquisition of the records of the Valley Peace Center and the papers of ethnographer Jozef Obrebski in 1973, the Library began to acquire collections of personal papers and organizational records of historical significance. The rare book and manuscript collections were combined administratively with the University Archives in the early 1990s to form the current Department of Special Collections and University Archives. Today, SCUA oversees a growing research collection of primary materials that includes rare books and manuscripts, historic maps, photographs, prints, and the official records of the campus at UMass Amherst.
North Center School District (Hatfield, Mass.)
North Center School District Records, 1818-1833.
Call no.: MS 442
The North Center School District in Hatfield, Massachusetts, was established in 1812, when the town divided into three school districts.
The collection consists of seventeen handwritten documents including financial records, a report and recipes relating to the North Center School District in Hatfield, Massachusetts, representing the period from 1818 to 1833. While not a comprehensive collection, the items nonetheless offer insight into education at the turn of the century, especially the sorts of expenses accrued in maintaining a small town schoolhouse.
- Education--Massachusetts--Hatfield
- Hatfield (Mass.)--History
- Massachusetts--History--1775-1865
- Recipes--Massachusetts
- School records--Massachusetts
- Schools--Records and Correspondence
- Allis, Dexter
- Bardwell, Elijah
- Bardwell, Remembrance
- Dickinson, Solomon
- Morton, Chester
- Morton, Jeremy
- North Center School District (Hatfield, Mass.)
- Porter, Theodore
- Waite, Daniel
- Waite, Justin


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Reprinted from Mises.org During the recent financial crisis, Sweden has emerged as one of very few financially sound economies. The country's strong position, setting it apart from most Western nations, makes it an interesting example of what could — or
The CBC is no good, and is not a Public Good
"In the beginning was The Hockey. And The Hockey was with the CBC. And The Hockey was the CBC." For almost all of us, "Hockey Night in Canada" was an essential part of growing up (or being grown up). It
B.C. First Nation Moves Toward Aboriginal Private Home Ownership
Reprinted from Frontier Centre for Public Policy This month, in a remote corner of northern B.C., just a few kilometres from the Alaskan border, three modest houses entered Canadian First Nations history. The residences, all located on the self-governed lands
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The 60th Anniversary of Orwell's 1984
Reprinted from the New American Sixty years following its first publication and twenty-five since the fateful year, George Orwell's 1984 remains a mystery to the experts. They convene often in exotic places to agree that Orwell wrote a dystopia on the communist
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Unemployment Benefits and the Disutility of Labor
Nineteenth century American politician and newspaper editor Gideon Tucker once wrote, "no man's life, liberty or property are safe while the Legislature is in session." The quote has been popularly attributed to Mark Twain, and is invoked in polemics damning
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Reforming the Canada Pension Plan
Amongst other ways that Canada is unique, it has a fully funded pension plan. Not too many countries can lay claim to this fact, and south of the border it is reported that only one (!) state pension is even
Generation Wars
I've written before on the raw deal Canadians are getting on the CPP, and it isn't pretty. I estimate that by being forcefully enrolled in to the Plan, Canadians are losing out on potentially earning an additional million over the
My letter to the NY Times re: You Call This Success?
Re: US Ends Bailout of GM Dear Sirs: Mr. Bill Vlasic and Ms. Annie L. Lowrey report that "Taxpayers lost about $10 billion on their $49.5 billion investment in (GM)." Their story is full of pride in achievement all the
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Kotlikoff on Unfunded Federal Liabilities, 2013: $205 Trillion
Reprinted from LewRockwell.com The nonpartisan Congressional Budget Office is acting in a bipartisan way to cover up the biggest single threat to the bipartisan political alliance that is stripping America of its wealth: the United States Congress. There is no
Canadian Artists Need to Swim on Their Own
Getting involved with politics is always a dicey move. This is especially so considering the only reason for seeking out the state is personal benefit. Whatever advantage one derives from the political class stays in a period of authoritative limbo,
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Why Redistribution Programs Trap the Poor
Reprinted from International Liberty I wrote last year about the way in which welfare programs lead to very high implicit marginal tax rates on low-income people. More specifically, they lose handouts when they earn income. As such, it is not very
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The French Choice: Soccer or Taxes
The list of France's national pastimes includes striking, but it also includes soccer. You can imagine the confusion/ire/angst generated by the announcement that the premier French league will strike at the end of November in protest of Socialist President Francois
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Whine and Cheese
First it was Canadian dairy producers who were upset with the proposed Canada-EU trade deal. Now it turns out Ontario's wine producers are also riled up. The proposed deal will make it cheaper for many goods to trade across the
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The new France – taxes for all, jobs for none
One in four French University graduates wants to leave the country for a better life. More than 70 percent of the French feel that taxes are "excessive" and 80 percent believe the country's economic policy is "misguided" and "inefficient." This
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Uruguay Legalization a Model for Canadian Pot?
I'm afraid so. Like I wrote in a previous post, legalizing marijuana gives the government more tax revenue and regulatory powers. This option should always be discouraged. But as more and more politicians jump on the pro-pot bandwagon, it's likely
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The Age of Hyperbole: How Normal Weather Became 'Extreme'
Reprinted from PJ Media Said Thomas Jefferson: "The man who reads nothing at all is better educated than the man who reads nothing but newspapers." Jefferson's comment may be expanded to include most of today's mass media; this is especially true
The Use of Knowledge at the Bank of Canada
The Bank of Canada's latest quarterly review suffers from the fatal conceit. Although technically the whole central banking system is a fatal conceit, one of the articles particularly highlights the complete ignorance and arrogance of Canada's central bankers. Nii Ayi
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The Criminality of the State
Reprinted from Mises.org As well as I can judge, the general attitude of Americans who are at all interested in foreign affairs is one of astonishment, coupled with distaste, displeasure, or horror, according to the individual observer's capacity for emotional
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Alleviating Separation Anxiety (Part 2)
…all human interaction, without exception, should be based on voluntary agreement. No one should be forced to associate with anyone else against his will. – Dr. Walter Block, Secession, 2007. In a previous posting, I highlighted the fact that separation for Québec
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A Plea for Public Property
Reprinted from FreeNation.org Public or Private? Libertarians often assume that a free society will be one in which all (or nearly all) property is private. I have previously expressed my dissent from this consensus, arguing that libertarian principles instead support


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Photo by Christina Holmes
Leave a Review
Great recipe! I took the advice of limiting the cardamon, would not use any at all because of the powerful flavor it has. Agree with doubling the cinnamon, and the dish is sweet enough without requiring maple sugar. Great breakfast alternative!
Do you know they make rolled quinoa? It looks just like a miniature version of oatmeal. I asked our local co-op to get it and they did. I love using it!
Couldnt be easier! Dumped everything into my cheap-o rice cooker and let it go. Warmed overnight, so at low temp, additional water could be absorbed. Sweet on its own, no need for honey or syrup, however I did add 2 tbs of walnut pieces for the omega three high five. Feels great to have my whole grains out of the way at breakfast!
I halved the recipe to make it the first time as I didn't want a whole pot I'd have to throw away if I didn't like it. However next time I'll make the whole recipe. I loved it! I will halve or eliminate the salt next time as I thought it had a bit too much of a salty taste. I will be making this with fresh apple and raisins in the future as well as the original way. Cardamon is very expensive (apprx $35 a pound) but if you go to a store with bulk spices you can buy just the amount you want.
Made exactly XC for no raisins. Did the morning cooking method; cooked for 30min, stirring every 5. For me, it did need a drizzle of maple syrup. Would add less salt next time Good and hearty.
I make this for myself already except for the Cardamon. I have been adding 1/2 tsp turmeric. Sometimes I use fresh fruit and for milk I use kefir. Also I add 1 tbl chia seeds.
I have made this several times using the overnight method and I think it is great. I use double cinnamon, no cardamom as I didn't have any and used different dried fruits. This has come out perfect every time. I also have not needed the maple syrup in the morning as the fruit was sweetener enough for our house. I have also added flax seeds as well. The only time I had an issues was when I ran out of quinoa and used all oats. Then the water amount was too much and I had to cook it again in the morning. This has become a winter staple in our house.
Hubby made this for the whole family for breakfast this morning, using the second method (not overnight). The kids (3 & 6) both asked for seconds and we finished off the rest. Hearty, interesting, & delicious! HOWEVER, we only used the recipe as inspiration. Everybody in our house likes different fruit mixed in, so we did that on an individual basis. And we don't have cardamom so we skipped that. Topped each bowl with sliced almonds, extra cinnamon, and a touch of honey instead of the maple syrup. Will definitely be making again!
This is a perfect breakfast for hot cereal and quinoa lovers, and I have made it several times. The low glycemic index of quinoa makes this combo stick with me longer, adds protein and I do like the taste of quinoa. Consistency is a bit loose as some have commented, but can just be cooked a bit longer or liquid can be reduced. I like adding milk later, so I reduced the water. I really love the sweetness and flavor of cardamom and cinnamon. Used dried cranberries or any other dried fruit as well as the raisins. I also add a big spoonful of ground flaxseed and whatever nuts we have around. No maple syrup needed but it could be a very good addition for kids. Note: I prefer the cooked on the morning of version
Interesting combination. I prepped it using the second method (simmer on the stove without leaving overnight) and used barberries. As an Iranian girl who doesn't cook Iranian food, I have plenty of barberries to spare. I think they are too sour to use alone: my mother didn't like it at all and my husband agreed that the barberries were a bit sour. In the future I think I'll simply use cranberries (I was out this time), or substitute some sweeter fruit such as figs or apricots. I liked the combination of the oats and quinoa as well as nuts and seeds, but I couldn't really taste the spices. The addition of maple syrup helped with the tartness. I found the result too watery when following the instructions so I let it boil a bit more at the end.


text:
When you woke up, sometime late at night, Ivan was there, sitting on the bed, watching you, the moon shining down on your face. You still clutched the sunflower in your hand.
"You're back
" you mumbled, guiltily happy. "And Feli
"They're not in the house anymore. The police was still unable to identify the murderer. The only thing they know for a fact is that it was the same person's doing."
That was
It was just so ho-horrible, and at my place, too... There
There won't be anymore murders, I hope
Ivan stared at you, a hint of darkness in those bewitching eyes. "Lovino and Antonio are gone."
You stared, dumbfounded. "ReReally?" you said in a small voice.
"Yes." The darkness in his eyes grew more profound. "There will not be any more murders, I think. After tonight, at least."
How do you know?" An unexplainable fear started to grip your body, but nothing was going to happen; this was Ivan, the only one you've ever loved, the one you could trust
He smiled down at you again, that smile that had comforted you after the death of all your friends, but there was something a bit off about it this time
No, you would not think that, this was Ivan
His words from earlier today rang through your head again; Trust me
Ivan bent his head down and kissed you; the deepest one that you had shared with him so far. Then, he unwound his scarf, and before you could fathom what he was doing, he had tied it around your mouth, tight.
"MMPHMM!" you screamed, but his scarf muffled it all.
"You see, I've been searching for one for so long, ___________." The dark, malicious gleam in his eyes grew stronger by the second. "A heart. Mine falls out sometimes, and I haven't been able to find it recently."
No, no, no, this couldn't
This wasn't happening. This was IVAN. You were just imagining it
Weren't you?
"Arthur's wasn't right. It was too focused on you, and too cold on the outside, but strangely warm on the inside. Then I thought Alfred's might work, so I got him while he was preparing for the funeraldidn't know that he was getting roses, not sunflowers though. Anyways, Alfred's was too focused on himself. But they showed so much 'kindness,' I think it's called, to you, but still-- their hearts weren't right. Then Francis came along, and I thought that maybe his would work, but no. It didn't. There was too much 'feeling' in it. Then that day Feliciano came in, blubbering, shedding
What's it called again? Tears? Right, tears. I thought maybe his heart would be suitable, what with his affection for that Ludwig, so I took Ludwig's heart for good measure, but it didn't work out either; theirs were too focused on each other, not to mention that Feliciano's heart was quite childish. 'Maybe it was a genetic thing or something,' I thought, so I just went over to his brother Lovino's, where Antonio was, also. Neither of them was fitting; it was the same thing with Feliciano and Ludwig'sthey had already belonged somewhere, to each other. I think I finally know the answer though. I finally know what it is. Love. Even though I don't have one of those 'hearts.' I love you, _____________. And I know you love me. I've found a place where your heart belongs. So dasveedanya. Good-bye. I'll love you always. And you'll love me, too. I'll make sure of it. Trust me."
The last thing you can remember is a steady chant of "Kolkolkol," as a sharp knife carefully cut out a hole in your chest, where your heart was located.


text:
Costume Photos
Costume Information
General | |
Cost : | 8.50 |
Description | |
Another Gijinka! I am planning to work with fur again, for legs and arm parts! I love working with fur hahah <3 I hope to get it done by the MCM Expo in May! |
Win! Looks fabulous :3
This is awesome xD
really cute!
But I would prefer it if you would wear a grey wig~
Thanks everyone! This cosplay was made just the night before the convention.
Hence, Hackfresse > I didn't manage to get a grey wig on time, even if I asked for one urgently on cosplayisland forums beforehand ^^;
N'aww this is so cute ^^ <3
Shopping List
Rainbow Tutu | £8.00 | To Buy |
Pink tank top | £3.00 | To Buy |
Total | £11.00 |
Update (Posted 14th May 2012) I changed my mind, I'm going to go for a rainbow coloured tutu, with a pink tank top that i'll do the dots to represent the pop tart! |


text:
What's New
Issue 11 have been includedSome bugs were fixed
App Description
Mom to be Magazine is Now available on iPhone for easy And Fast Access!You will have all our previous issues for free Very Soon.
Our Magazine covers your journey as mom from the beginning of your pregnancy and throughout your child Stimulating Life!!
Mom2be is a complete Guide that explore all aspect and topics related to your motherhood, such as: health, life style, nutrition, education and even entertaining yourself and your baby, we have it all
mom2be, with you all the way!!
App Changes
May 18, 2014 New version 2.2
January 29, 2014 New version 2.1
September 26, 2013 New version 2.0
May 06, 2013 New version 1.1
November 30, -0001 Initial Release


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The support and dedication WNED has received from our Southern Ontario viewers has enabled the station to enrich countless lives through the quality and impact of public television. Read More.
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WNED Buffalo / Toronto
Position Title: Corporate Development Sales Representative
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This is a discussion on Non-Standard and Custom Holster Options -- thigh, bra, fanny pack, purse etc. within the Defensive Carry Holsters & Carry Options forums, part of the Defensive Carry Discussions category; Originally Posted by merischino They wouldn't find this ultimately irritating? I'd probably put their holsters on upside down and put my gun in backwards.... (really!) ...
Thigh holster
Bra holster
Belly Band
Purse holster
Fanny Pack
Other (underwear, no holster, whatever)
Fortune favors the bold.
Freedom doesn't mean safe, it means free.
The thing about "defense" is that it has practically nothing to do with guns. (As passed on by CCW9MM)
...erm.... I fed the bullets into a Glock magazine backwards and then managed to get the magazine stuck halfway into the gun, backwards?
In my defense, I did aim the front of the bullets towards the front of the gun... !
Also in my defense, this was among the first handful of times I'd ever been to a range, and perhaps the second actual day I'd been to a range, and the very first time I'd been left by my lonesome in the range to do everything without guidance. (K was outside allegedly watching through the glass.) Also in my defense, I'd enrolled in a class but was then and am still waiting for it actually to be held... that will be next week.
LOLOL OK, that's great. I know that it took me awhile to remember which way the bullets went..I'd stand there like I was dyslexic, holding the bullet one way, then the other, imagining which way the mag went into the gun, then which way the barrel pointed...to figure out which way the bullet should go in. But I dont think my mags would take the bullets backwards.
But I was able to do it in private, in the individual gun bay on the firing line.
On that note, esp. since you are interested in high capacity mags: YouTube - Universal Pistol Magazine Loader
Uplula, totally worth the $$$.
Fortune favors the bold.
Freedom doesn't mean safe, it means free.
The thing about "defense" is that it has practically nothing to do with guns. (As passed on by CCW9MM)
I am really liking the Raven model cross-shoulder bag offered by the Gun Toten Mamas. With Pax's recommendation and comments regarding quality, and the attractive price (not the lowest, but for good quality... that's an excellent price).
Does anyone own one of these?
One thing I wonder about with gun purses/fanny packs is the look. There don't seem to be that many different types out there. So, when you are walking down the street is every BG going to immediately see you and think, "that's a gun purse"?
I've heard some people mention that "fanny packs scream CCW"...
Maybe BGs don't pay that much attention, or maybe they do?
"Trust in God with hand on sword" -Inscription on my family's coat of arms from medieval England
---Carry options: G26/MTAC, PF9/MiniTuck, PPK/Pocket, USP40/OWB---
---NOTE: I am not an expert. If I ever start acting like a know-it-all, please call me on it immediately. ---
Well dont worry to much about (it looks like a gun purse) most people will never notice, maybe a gun person.
My wife slickshot also a member here carries a Glock 19
most of the time in a High noon IWB or a fanny pack.
She is 5'-9" 109 lb's soaking wet and pulls it off really well.
Even in shorts or tight jeans, with a wife beater and a bloused shirt everthig disappears.
We love to bike ride a lot and a fanny pack is the best option that we have found, both of us did the Duval craw in Key West last summer and no one ever ask or suspected that we both were armed.
Just me, I would never advise off carry in a purse for the obvious reasons.
"A lie gets halfway around the world before the truth has a chance to get its pants on."
- Sir Winston Churchill
Raven is my favorite out of that whole line!
It's small enough to be very convenient, but large enough to tote a full-size 1911 vertically if that's what floats your boat. A G22 would almost certainly fit in there--I can check with a G20 later today if you'd like?
And it doesn't look like a gun bag at all, just like a sporty little tote pack.
They've recently moved the holster-attaching Velcro from the outer inside wall to the inner inside wall (eg, so it is now attached to the wall closest to the user's body, instead of to the wall of the panel that opens). IMO, that's a major improvement. Their designer also tells me that in June they will add rip-pull tabs to the upper corners, so it can function more like a fast-action fanny pack if you want to set it up for a rip-and-grab vertical draw. But the tabs will be easily tucked down inside if you'd rather set it up to maintain more stealth.
Either way, I'm quite pleased with both the product as it is now and with the direction the designer is going. She's been in the leather bags & totes business for years and years, but recently got into producing concealed carry products and is asking all the right questions. Very sharp lady.
Kathy Jackson
My website: Cornered Cat
There are many purses on the market that don't look like a gun purse. See, for example, Designer Concealed Carry or Ladies Protection - CCW Purse - Products Page (esp. the "Slimline") or Galco holsters; Holsters; Gun holster, pistol holsters, western holsters, shoulder holsters, leather holster and Glock holsters
Kathy Jackson
My website: Cornered Cat
Fortune favors the bold.
Freedom doesn't mean safe, it means free.
The thing about "defense" is that it has practically nothing to do with guns. (As passed on by CCW9MM)
Well I wouldn't be too embarassed. I bought the UpLULA magazine reloader and managed when not paying attention to load 4 rounds into the magazine..backwards..Now that was truly embarassing!! And yeah I'm the nerd that put his thumb in the wrong hole on the bowling ball and wound up going halfway down the lane !
Make you feel better?
Walther P99 AS full size 9mm
Kahr P9 compact 9mm
Michigan Sheriff's Association
The key is to hit them hard, hit them fast, and hit them repeatedly. The one shot stop is a unit of measurement not a tactical philosophy. Evan Marshall
Guys, you are all awesome and thank you all so much for responding to and continuing to read this thread.
Pax -- if you wouldn't mind trying that G20 in the Raven, I would be much obliged. And thanks for the info on what GTM are planning with design changes to the Raven. Rather than rush to buy one now it might behoove me to wait (as was my original plan anyway) as I think I'd like those tabs and the option for a rushed draw they provide. I did send them an email from their site asking what availability/delivery times are right now.
Mel - That makes me feel a whole lot better. Thanks!
9MMare - I have similar thoughts as you do re: the purse. I happen to have a few different purses. The big clunky ones I get as gifts and rarely if ever carry. The medium one-shouldered ones just don't fit my body well, so I don't wear them. The very small, whether stylish or not, tend to get used. Sometimes, that's a hand-carry style with straps large enough only for hands to go through, and sometimes. Most of the time. Arguably 99% of the time, they are large enough only for a wallet, a notepad, a pen, and the collection of receipts as I go about my day, and are cross-shoulder in design.
Of all the purses I've seen at GTM, The Raven is the closest to this last and most used purse design. Also, Coronado has a couple of cross shoulders that are attractive, if a little big and expensive for me. The "tactical" cross-shoulder bag you discussed earlier in this thread, with all its nylon and zippers etc. was a super attractive option for me. Granted, I no longer live in the Pacific Northwest so it's ultra-durable look probably won't fit with my wardrobe as well as it would have when I lived way out yonder near you, but it's a great design and I liked it very much.
Ron -- my bf Kevin is a cycling fanatic. I have been thinking about purchasing him a SmartCarry or perhaps Thunderwear so that he can carry daily as he goes about his cycling escapades. Do you have any experience with either of these? Also... you mention fanny packs being your carry preference. Do you have a particular model you use (e.g., Tommy's Gun Packs, other) or do you just use a generic fanny pack that is not necessarily designed to be used as a holster?
GWRedDragon -- this was my same question. Thanks for asking it!
Mel -- forgot to ask. What is an UpLULA magazine reloader? Do you have a link for it?
Merischino, here is a video link of the upLula on action.
I couldn't be without mine.
YouTube - Universal Pistol Magazine Loader
Disclaimer: The posts made by this member are only the members opinion, not a reflection on anyone else, nor the group, and should not be cause for anyone to get their undergarments wedged in an uncomfortable position.
Wow! I'm dumbfounded! How much does one cost, generally?
Fortune favors the bold.
Freedom doesn't mean safe, it means free.
The thing about "defense" is that it has practically nothing to do with guns. (As passed on by CCW9MM)
View Poll Results: What is your preferred non-standard Holster/Carry option?
Thigh holster 2 5.00%
Bra holster 1 2.50%
Belly Band 4 10.00%
Purse holster 5 12.50%
Fanny Pack 16 40.00%
Other (underwear,
no holster, whatever) 15 37.50%
Multiple Choice Poll. Voters: 40.
Recognizing that our sample size is not statistically significant, the above is interesting to me. I had assumed that in the "non-standard holster" conversation, "other" would be the smallest/least represented poll response.
I didn't put a whole lot of thought into what types of holsters might fall in there. Anyone want to give me an idea what does? I'm hoping it isn't just a catch-all being used for the response "I use a standard, not a non-standard, holster" but I'm guessing that may be what's happening.
Also, not a whole lot of thigh holster respondents. Does anybody have first-hand experience (even if not EDC experience) with thigh holsters that they'd care to share? I have my eye on a custom thigh holster from Guns 4 Gals and would love some feedback, any at all.
Thanks again guys!


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Need To Know | BP's "other" disaster | PBS
Duration: 12:20
Need to Know #110
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Fractions - Recipe project
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I want to teach the students a real life way to use fractions with cooking recipes. I also want them to convert the recipe so they can use the skill of adding fractions. Has anyone done something similar to this? I'm looking for ideas as to how to get it started, make a rubric, and wondering whether to make it an in class or home project. Thanks!


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Home → Maine Resources → Maine Writers Search → Maine Writers List → A Maine Writer
Craig, Barbara ( - )
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Selected Bibliography
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