text:
Publications (124)377.82 Total impact
- [Show abstract] [Hide abstract]
ABSTRACT: Although MPP(+) (1-methyl-4-phenylpyridinium) has been widely used to damage dopaminergic neurons of the Substantia Nigra pars compacta (SNc) and produce animal and cellular models of Parkinson's disease, the action of this toxin on ion channels and electrophysiological properties of these neurons remains controversial. Previous work has attributed the early effects of MPP(+) on the membrane potential and firing frequency of SNc neurons either to block of hyperpolarisation-activated (Ih) current, or to activation of ATP-sensitive K(+) (KATP) channels. Using a combination of electrophysiological and pharmacological techniques, we investigated the acute effects of MPP(+) (20μM) on SNc neurons in rat midbrain slices. Our results show that MPP(+) inhibits the activity of these neurons in distinct stages involving different mechanisms. The early phase of inhibition was dependent on D2 autoreceptors, but [(3)H]raclopride membrane binding and cAMP production assays demonstrated that the toxin (0.001-100μM) did not directly bind to these receptors nor activated the Gi-linked signalling pathway. Depletion of vesicular dopamine with Ro4-1284 attenuated the early inhibitory effect, indicating that D2 autoreceptors were activated by dopamine released from the somato-dendritic region. After longer exposure (>10-20min), MPP(+) produced a late phase of inhibition which mainly involved activation of KATP channels, and required uptake of the toxin via dopamine transporter. Although Ih current mediated by hyperpolarisation-activated cyclic nucleotide-gated (HCN) channels was reduced by MPP(+), neither inhibition of firing nor membrane potential hyperpolarisation were significantly attenuated by blocking HCN channels with ZD7288. Our results indicate that the initial cellular events that lead to activation of cell death pathways by MPP(+) are complex and include KATP, and dopamine-dependent components, and show that the inhibitory effect of the toxin is independent of Ih block.NeuroToxicology 12/2014; · 3.05 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Previous studies suggested that amyloid β (Aβ)-induced disruption of astrocytic Ca(2+) signalling and oxidative stress play a major role in the progression towards neuronal and glial death in Alzheimer's disease. We have recently demonstrated that Ca(2+)-permeable TRPV4 channels are highly expressed in rat hippocampal astrocytes and are involved in oxidative stress-induced cell damage. The aim of this study was to test the hypothesis that TRPV4 channels also contribute to hippocampal damage evoked by Aβ. Synthetic Aβ40 evoked cell death in hippocampal slice cultures in a concentration (0-20μM) and time (12-48 hr) dependent manner, after cultures were preconditioned with sublethal concentration of buthionine sulfoximine (1.5μM) which enhanced endogenous ROS production. As demonstrated by propidium iodide fluorescence, damage was observed in the granule cell layer of the dentate gyrus and to a smaller degree in pyramidal neurons of the CA1-CA3 region, as well as in glia cells mainly at the edge of the slice. Immunocytochemistry revealed an altered pattern of TRPV4 and GFAP protein expression, and reactive astrogliosis surrounding pyramidal CA1-CA3 neurons. Neuronal and astrocytic damage was attenuated by the antioxidant Trolox, TRPV4 channel blockers Gd(3+) and ruthenium red (RR), and a specific inhibitor of the redox and Ca(2+)-sensitive phospholipase A2 enzyme (MAFP). In disassociated co-cultures of hippocampal neurons and astrocytes without BSO preconditioning, Aβ40 evoked pronounced neuronal damage, enhanced the expression of TRPV4 and GFAP proteins (indicative of reactive astrogliosis), and increased intracellular free Ca(2+) concentration in astrocytes. The latter effect was attenuated by RR and in Ca(2+)-free media. These data show that Aβ40 can activate astrocytic TRPV4 channels in the hippocampus, leading to neuronal and astrocytic damage in a Ca(2+) and oxidative stress-dependent manner.NeuroToxicology 01/2014; · 3.05 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Prior to the "onset of hearing," developing cochlear inner hair cells (IHCs) and primary auditory neurons undergo experience-independent activity, which is thought to be important in retaining and refining neural connections in the absence of sound. One of the major hypotheses regarding the origin of such activity involves a group of columnar epithelial supporting cells forming Kölliker's organ, which is only present during this critical period of auditory development. There is strong evidence for a purinergic signalling mechanism underlying such activity. ATP released through connexin hemichannels may activate P2 purinergic receptors in both Kölliker's organ and the adjacent IHCs, leading to generation of electrical activity throughout the auditory system. However, recent work has suggested an alternative origin, by demonstrating the ability of IHCs to generate this spontaneous activity without activation by ATP. Regardless, developmental abnormalities of Kölliker's organ may lead to congenital hearing loss, considering that mutations in ion channels (hemichannels, gap junctions, and calcium channels) involved in Kölliker's organ activity share strong links with such types of deafness.BioMed Research International 01/2014; 2014:367939. · 2.71 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Endocannabinoids (eCBs) modulate synaptic transmission in the brain, but little is known of their regulatory role in nigral dopaminergic neurons, and whether transmission to these neurons is tonically inhibited by eCBs as seen in some other brain regions. Using whole-cell recording in midbrain slices, we observed potentiation of evoked IPSCs (eIPSCs) in these neurons after blocking CB1 receptors with rimonabant or LY-320,135, indicating the presence of an eCB tone reducing inhibitory synaptic transmission. Increased postsynaptic calcium buffering and block of mGluR1 or postsynaptic G-protein coupled receptors prevented this potentiation. Increasing spillover of endogenous glutamate by inhibiting uptake attenuated eIPSC amplitude, while enhancing the potentiation by rimonabant. Group I mGluR activation transiently inhibited eIPSCs, which could be prevented by GDP-β-S, increased calcium buffering or rimonabant. We explored the possibility that the dopamine-derived eCB N-arachidonoyl dopamine (NADA) is involved. The eCB tone was abolished by preventing dopamine synthesis, and enhanced by L-DOPA. It was not detected in adjacent non-dopaminergic neurons. Preventing 2-AG synthesis did not affect the tone, while inhibition of NADA production abolished it. Quantification of ventral midbrain NADA suggested a basal level that increased following prolonged depolarization or mGluR activation. Since block of the tone was not always accompanied by attenuation of depolarization-induced suppression of inhibition (DSI) and vice versa, our results indicate DSI and the eCB tone are mediated by distinct eCBs. This study provides evidence that dopamine modulates the activity of SNc neurons not only by conventional dopamine receptors, but also by CB1 receptors, potentially via NADA.Neuropharmacology 12/2013; · 4.82 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Spreading depression (SD) is a profound depolarization of neurons and glia that propagates in a wave-like manner across susceptible brain regions, and can develop during periods of compromised cellular energy such as ischemia, when it influences the severity of acute neuronal damage. Although SD has been well characterised in the cerebral cortex and hippocampus, little is known of this event in the Substantia Nigra (SN), a brainstem nucleus engaged in motor control and reward-related behaviour. Transverse brain slices (250μm; P21-23 rats) containing the SN were subject to oxygen and glucose deprivation (OGD) tests, modelling brain ischemia. SD developed in lateral aspects of the SN within 3.3±0.2min of OGD onset, and spread through the Substantia Nigra pars reticulata (SNr), as indicated by fast-occurring and propagating increased tissue light transmittance and negative shift of extracellular DC potential. These events were associated with profound mitochondrial membrane depolarization (ΔΨm) throughout the SN, as demonstrated by increased Rhodamine 123 fluorescence. Extracellular recordings from individual SNr neurons indicated rapid depolarization followed by depolarizing block, while dopaminergic neurons in the Substantia Nigra pars compacta (SNc) showed inhibition of firing associated with hyperpolarization. SD evoked in the SNr was similar to OGD-induced SD in the CA1 region in hippocampal slices. In the hippocampus, SD also developed during anoxia or aglycemia alone (associated with less profound ΔΨm than OGD), while these conditions rarely led to SD in the SNr. Our results demonstrate that OGD consistently evokes SD in the SN, and that this phenomenon only involves the SNr. It remains to be established whether nigral SD contributes to neuronal damage associated with a sudden-onset form of Parkinson's disease known as 'vascular parkinsonism'.Brain research 06/2013; · 2.83 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: l-DOPA (Levodopa) remains the gold standard for the treatment of motor symptoms of Parkinson's disease (PD), despite indications that the drug may have detrimental effects in cell culture. Classically, l-DOPA increases the production of dopamine (DA) in nigral dopaminergic neurons, while paradoxically inhibiting the firing of these neurons due to activation of D2 autoreceptors by extracellularly released DA. Using a combination of electrophysiology and calcium microfluorometry in brain slices, we have identified a novel effect of l-DOPA on dopaminergic neurons when D2 receptors were blocked. Under these conditions, l-DOPA (0.03-3mM) evoked an excitatory effect consisting of two components. The 'early' component observed during and immediately after application of the drug, was associated with increased firing, membrane depolarization and inward current. This excitatory response was strongly attenuated by CNQX (10μM), pointing to the involvement of TOPA quinone, an auto-oxidation product of l-DOPA and a potent activator of AMPA/kainate receptors. The 'late' phase of excitation persisted >30min after brief l-DOPA application and was not mediated by ionotropic glutamate receptors, nor by D1, α1-adrenergic, mGluR1 or GABAB receptors. It was eliminated by carbidopa, demonstrating its dependence on conversion of l-DOPA to DA. Exogenous DA (50μM) also evoked a glutamate-receptor independent increase in firing and an inward current when D2 receptors were blocked. In voltage-clamped neurons, both l-DOPA and DA produced a long-lasting increase in [Ca2+]i which was unaffected by block of ionotropic glutamate receptors. These results demonstrate that l-DOPA has dual, inhibitory and excitatory, effects on nigral dopaminergic neurons, and suggest that the excitation and calcium rise may have long-lasting consequences for the activity and survival of these neurons when the expression or function of D2 receptors is impaired.Experimental Neurology 02/2013; · 4.62 Impact Factor -
Conference Paper: ACUTE EFFECTS OF ROTENONE ON LOCUS COERULEUS AND SUBSTANTIA NIGRA PARS COMPACTA NEURONS: IMPLICATIONS FOR PARKINSON'S DISEASE
[Show abstract] [Hide abstract]
ABSTRACT: Purpose: While the major hallmark of Parkinson's disease (PD) is relatively selective degeneration of dopaminergic neurons in the Substantia Nigra pars compacta (SNc), there is also substantial loss of noradrenergic Locus Coeruleus (LC) neurons. The loss of these neurons may contribute to non-motor symptoms of PD which often occur earlier in the disease than characteristic motor symptoms. Based on the theory that LC neurons are damaged earlier in the progression of PD, we hypothesize that these neurons are more vulnerable to neurotoxic insult than SNc neurons. Methods: Using electrophysiological techniques, we have directly compared the responses of LC and SNc neurons in acute brain slices to rotenone, a mitochondrial complex I inhibitor, widely used to produce animal models of PD. Results: Rotenone (0.1-5 μM) produced a dose-dependent (p<0.05) decrease in the spontaneous firing of LC and SNc neurons recorded extracellularly, associated with cell membrane hyperpolarisation and a tolbutamide (100 μM)-sensitive outward current in whole cell patch clamp recordings. These effects were largely mediated by ATP-sensitive K+ (KATP) channels, the activation of which was greater in SNc neurons than LC neurons (p<0.01). When KATP channels were blocked with tolbutamide, rotenone (1 μM) increased the firing of LC and SNc neurons. This effect which was associated with an inward current, unmasked by intracellular Cs+ , which effectively blocks all K+ conductances. Conclusion: Rotenone activates KATP channels more strongly in SNc neurons than in the LC, potentially protecting these neurons from detrimental effects of mitochondrial toxins such as rotenone. Thus LC neurons may be more susceptible to neurotoxininduced damage than SNc neurons.ANS Annual Conference; 02/2013 - [Show abstract] [Hide abstract]
ABSTRACT: Transient receptor potential melastatin 2 (TRPM2) channels are sensitive to oxidative stress, and their activation can lead to cell death. Although these channels have been extensively studied in expression systems, their role in the brain, particularly in the substantia nigra pars compacta (SNc), remains unknown. In this study, we assessed the expression and functional properties of TRPM2 channels in rat dopaminergic SNc neurons, using acute brain slices. RT-PCR analysis revealed TRPM2 mRNA expression in the SNc region. Immunohistochemistry demonstrated expression of TRPM2 protein in tyrosine hydroxylase-positive neurons. Channel function was tested with whole cell patch-clamp recordings and calcium (fura-2) imaging. Intracellular application of ADP-ribose (50-400 μM) evoked a dose-dependent, desensitizing inward current and intracellular free calcium concentration ([Ca(2+)](i)) rise. These responses were strongly inhibited by the nonselective TRPM2 channel blockers clotrimazole and flufenamic acid. Exogenous application of H(2)O(2) (1-5 mM) evoked a rise in [Ca(2+)](i) and an outward current mainly due to activation of ATP-sensitive potassium (K(ATP)) channels. Inhibition of K(+) conductance with Cs(+) and tetraethylammonium unmasked an inward current. The inward current and/or [Ca(2+)](i) rise were partially blocked by clotrimazole and N-(p-amylcinnamoyl)anthranilic acid (ACA). The H(2)O(2)-induced [Ca(2+)](i) rise was abolished in "zero" extracellular Ca(2+) concentration and was enhanced at higher baseline [Ca(2+)](i), consistent with activation of TRPM2 channels in the cell membrane. These results provide evidence for the functional expression of TRPM2 channels in dopaminergic SNc neurons. Given the involvement of oxidative stress in degeneration of SNc neurons in Parkinson's disease, further studies are needed to determine the pathophysiological role of these channels in the disease process.Journal of Neurophysiology 09/2011; 106(6):2865-75. · 3.04 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: There is consensus that amelioration of the motor symptoms of Parkinson's disease is most effective with L-DOPA (levodopa). However, this necessary therapeutic step is biased by an enduring belief that L-DOPA is toxic to the remaining substantia nigra dopaminergic neurons by itself, or by specific metabolites such as dopamine. The concept of L-DOPA toxicity originated from pre-clinical studies conducted mainly in cell culture, demonstrating that L-DOPA or its derivatives damage dopaminergic neurons due to oxidative stress and other mechanisms. However, the in vitro data remain controversial as some studies showed neuroprotective, rather than toxic action of the drug. The relevance of this debate needs to be considered in the context of the studies conducted on animals and in clinical trials that do not provide convincing evidence for L-DOPA toxicity in vivo. This review presents the current views on the pathophysiology of Parkinson's disease, focusing on mitochondrial dysfunction and oxidative/proteolytic stress, the factors that can be affected by L-DOPA or its metabolites. We then critically discuss the evidence supporting the two opposing views on the effects of L-DOPA in vitro, as well as the animal and human data. We also address the problem of inadequate experimental models used in these studies. L-DOPA remains the symptomatic 'hero' of Parkinson's disease. Whether it contributes to degeneration of nigral dopaminergic neurons, or is a 'scapegoat' for explaining undesirable or unexpected effects of the treatment, remains a hotly debated topic.Progress in Neurobiology 06/2011; 94(4):389-407. · 10.30 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Organotypic cultures (OCs) have been widely used to investigate the midbrain dopaminergic system, but only a few studies focused on the functional properties of dopaminergic neurons and their synaptic inputs from dopaminergic and non-dopaminergic neurons also contained in such cultures. In addition, it is not clear whether the culturing process affects the intrinsic neuronal properties and the expression of specific receptors and transporters. We performed patch-clamp recordings from dopaminergic neurons in mesencephalic-striatal co-cultures obtained from transgenic mice expressing green fluorescent protein (GFP) under the tyrosine hydroxylase promoter. Some (10/44) GFP+ neurons displayed a bursting activity that renders the firing of these cells similar to that of the dopaminergic neurons in vivo. The culturing process reduced the hyperpolarization-activated current (I(h) ) and the expression of D₂ receptors. Downregulation of D₂ receptor mRNA and protein was confirmed with reverse transcriptase polymerase chain reaction and Western blotting. Immunocytochemistry revealed that many synaptic terminals, most likely originating from dopaminergic neurons, co-expressed the dopamine (DA) transporter and the vesicular glutamate transporter-2, suggesting a co-release of DA and glutamate. Interestingly, exogenous DA decreased glutamate release in young cultures [days in vitro (DIV)<20] by acting on pre-synaptic D₂ receptors, while in older cultures (DIV>26) DA increased glutamate release by acting on α-1 adrenoreceptors. The facilitatory effect of DA on glutamatergic transmission to midbrain dopaminergic neurons may be important in conditions when the expression of D₂ receptors is compromised, such as long-term treatment with antipsychotic drugs. Our data show that midbrain OCs at DIV>26 may provide a suitable model of such conditions.European Journal of Neuroscience 03/2011; 33(9):1622-36. · 3.67 Impact Factor - 01/2011;
- [Show abstract] [Hide abstract]
ABSTRACT: A reliable model system of epileptiform insult would facilitate investigation into the underlying biological mechanisms. Epileptiform insult was induced in hippocampal slice cultures by lowering extracellular Mg(2+), (+)-bicuculline, or (-)-bicuculline methochloride, a stable salt form of bicuculline (both forms block GABA(A) receptors). Cell death was assessed by propidium iodide uptake. Low Mg(2+) or (+)-bicuculline did not produce cell death regardless of dose or incubation period. Exposure to 100 microM (-)-bicuculline methochloride for 48 hr resulted in prominent CA1 cell death. These findings demonstrate that not all pro-epileptic drugs/ion changes used routinely for electrophysiological recording of seizure activity lead to cell death in hippocampal slice cultures and that treatment with bicuculline methochloride can be used as a reliable model for epileptiform insult.The International journal of neuroscience 10/2010; 120(12):752-9. · 1.53 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: TRPM2 and TPPV4 channels, two members of TRP channel family, are known to be widely expressed in the brain but their exact expression pattern and function are not well understood. Due to their high Ca(2+) permeability and gating by reactive oxygen species (TRPM2), or cell swelling, low pH and high temperature (TRPV4), they are likely to be involved in cell damage associated with various brain pathologies. The aim of this study was to investigate the expression of these channels and their potential role in oxidative stress-induced cell damage in organotypic hippocampal slice cultures, a model that retains the complex interaction between neurons and astrocytes. Channel expression was confirmed with RT-PCR and western blotting, while immunocytochemistry demonstrated TRPM2 in CA1-CA3 pyramidal neurons and TRPV4 in astrocytes. Oxidative stress induced by exogenous application of H(2)O(2) (600 microM) caused preferential damage of pyramidal neurons, while oxidative stress evoked with mercaptosuccinate (MCS; 400 microM) or buthionine sulfoximine (BSO; 4 microM) mainly damaged astrocytes, as identified by propidium iodide fluorescence. Antioxidants (Trolox 500 microM; MitoE 2 microM) reduced both neuronal and astrocytic cell death. Blockers of TRPV4 channels (Gd(3+) 500 microM; Ruthenium red 1 microM) increased the viability of astrocytes following MCS or BSO treatments, consistent with the expression pattern of these channels. Blockers of TRPM2 channels clotrimazole (20 microM), N-(p-amylcinnomoyl)anthranilic acid (ACA, 25 microM) or flufenamic acid (FFA, 200 microM) failed to protect pyramidal neurons from damage caused by exogenous H(2)O(2), and increased damage of these neurons caused by MCS and BSO. The differential expression of stress-sensitive TRPM2 and TRPV4 channels in hippocampal neurons and astrocytes that show distinct differences in vulnerability to different forms of oxidative stress suggests the specific involvement of these channels in oxidative stress-induced cell damage. However, the exact relationship between TRPM2 channel activation and cell death still remains to be determined due to the lack of protective effects of TRPM2 channel blockers.NeuroToxicology 03/2010; 31(2):204-14. · 3.05 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Pauses in the tonic firing of striatal cholinergic interneurons emerge during reward-related learning and are triggered by neutral cues which develop behavioural significance. In a previous in vivo study we have proposed that these pauses in firing may be due to intrinsically generated afterhyperpolarisations (AHPs) evoked by excitatory synaptic inputs, including those below the threshold for action potential firing. The aim of this study was to investigate the mechanism of the AHPs using a brain slice preparation which preserved both cerebral hemispheres. Augmenting cortically evoked postsynaptic potentials (PSPs) by repetitive stimulation of cortical afferents evoked AHPs that were unaffected by blocking either GABA(A) receptors with bicuculline, or GABA(B) receptors with saclofen or CGP55845. Apamin (a blocker of small conductance Ca(2+)-activated K(+) channels) had minimal effects, while chelation of intracellular Ca(2+) with BAPTA reduced the AHP by about 30%. In contrast, blocking hyperpolarisation and cyclic nucleotide activated (HCN) cation current (I(H)) with ZD7288 or Cs(+) diminished the size of the AHPs by 60% and reduced the proportion of episodes that contained this hyperpolarisation. The reversal potential (20 mV) and voltage dependence of the AHPs were consistent with the hypothesis that a transient deactivation of I(H) caused most of the AHP at hyperpolarised potentials, while the slow AHP-type Ca(2+)-activated K(+) channels increasingly contributed at more depolarised membrane potentials. Subthreshold somatic current injections yielded similar AHPs with a median duration of approximately 700 ms that were not affected by firing of a single action potential. These results indicate that transient deactivation of HCN channels evokes pauses in tonic firing of cholinergic interneurons, an event likely to be elicited by augmentation of afferent synaptic inputs during learning.The Journal of Physiology 11/2009; 587(Pt 24):5879-97. · 4.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Rotenone is a toxin used to generate animal models of Parkinson's disease; however, the mechanisms of toxicity in substantia nigra pars compacta (SNc) neurons have not been well characterized. We have investigated rotenone (0.05-1 microm) effects on SNc neurons in acute rat midbrain slices, using whole-cell patch-clamp recording combined with microfluorometry. Rotenone evoked a tolbutamide-sensitive outward current (94 +/- 15 pA) associated with increases in intracellular [Ca(2+)] ([Ca(2+)](i)) (73.8 +/- 7.7 nm) and intracellular [Na(+)] (3.1 +/- 0.6 mm) (all with 1 microm). The outward current was not affected by a high ATP level (10 mm) in the patch pipette but was decreased by Trolox. The [Ca(2+)](i) rise was abolished by removing extracellular Ca(2+), and attenuated by Trolox and a transient receptor potential M2 (TRPM2) channel blocker, N-(p-amylcinnamoyl) anthranilic acid. Other effects included mitochondrial depolarization (rhodamine-123) and increased mitochondrial reactive oxygen species (ROS) production (MitoSox), which was also abolished by Trolox. A low concentration of rotenone (5 nm) that, by itself, did not evoke a [Ca(2+)](i) rise resulted in a large (46.6 +/- 25.3 nm) Ca(2+) response when baseline [Ca(2+)](i) was increased by a 'priming' protocol that activated voltage-gated Ca(2+) channels. There was also a positive correlation between 'naturally' occurring variations in baseline [Ca(2+)](i) and the rotenone-induced [Ca(2+)](i) rise. This correlation was not seen in non-dopaminergic neurons of the substantia nigra pars reticulata (SNr). Our results show that mitochondrial ROS production is a key element in the effect of rotenone on ATP-gated K(+) channels and TRPM2-like channels in SNc neurons, and demonstrate, in these neurons (but not in the SNr), a large potentiation of rotenone-induced [Ca(2+)](i) rise by a small increase in baseline [Ca(2+)](i).European Journal of Neuroscience 11/2009; 30(10):1849-59. · 3.67 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: P2 receptor (R) signalling plays an important role in the central ventilatory response to hypoxia. The frequency increase that results from activation of P2Y(1)Rs in the preBötzinger complex (preBötC; putative site of inspiratory rhythm generation) may contribute, but neither the cellular nor ionic mechanism(s) underlying these effects are known. We applied whole-cell recording to rhythmically-active medullary slices from neonatal rat to define, in preBötC neurones, the candidate cellular and ionic mechanisms through which ATP influences rhythm, and tested the hypothesis that putative rhythmogenic preBötC neurones are uniquely sensitive to ATP. ATP (1 mm) evoked inward currents in all non-respiratory neurones and the majority of respiratory neurons, which included inspiratory, expiratory and putative rhythmogenic inspiratory neurones identified by sensitivity to substance P (1 microM) and DAMGO (50 microM) or by voltage-dependent pacemaker-like activity. ATP current densities were similar in all classes of preBötC respiratory neurone. Reversal potentials and input resistance changes for ATP currents in respiratory neurones suggested they resulted from either inhibition of a K(+) channel or activation of a mixed cationic conductance. The P2YR agonist 2MeSADP (1 mm) evoked only the latter type of current in inspiratory and pacemaker-like neurones. In summary, putative rhythmogenic preBötC neurones were sensitive to ATP. However, this sensitivity was not unique; ATP evoked similar currents in all types of preBötC respiratory neurone. The P2Y(1)R-mediated frequency increase is therefore more likely to reflect activation of a mixed cationic conductance in multiple types of preBötC neurone than excitation of one, highly sensitive group.The Journal of Physiology 04/2008; 586(5):1429-46. · 4.38 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Calpains are Ca(2+)-activated enzymes which cleave cytoskeletal and other proteins, contributing to neuronal damage in conditions of pathological intracellular Ca(2+) elevation, including stroke. However, the consequences of calpain overactivation have typically been observed hours after insult. To identify the earliest events attributable to calpain activation, and thus potentially isolate calpain substrates involved in acute neuronal damage, we dynamically recorded the effects of calpain inhibition in an in vitro model of stroke. Extracellular DC potentials and fEPSPs were monitored together with changes of light transmittance (as a measure of cell and mitochondrial swelling) and Rh 123 fluorescence (to monitor mitochondrial membrane potential; DeltaPsi(m)) in hippocampal slices obtained from P12-P17 rats. No differences were observed in the latencies of fEPSP disruption or onset of extracellular DC shifts associated with hypoxic spreading depression (HSD) evoked by oxygen-glucose deprivation (OGD) under control conditions or in the presence of calpain inhibitor III (MDL 28170). However, a significant difference was observed in transmitted light signals during OGD with calpain inhibition. Given the potential contribution of mitochondrial swelling to changes in light transmittance, these experiments were also conducted in the presence of cyclosporin A to block opening of the mitochondrial permeability transition pore (MPTP). Our results indicate that differences in OGD-induced changes of light transmittance in the presence of MDL 28170 are not likely the result of MPTP blockade or changes in dendritic beading. We propose that calpain inhibition may alter changes in light transmittance by limiting conformational changes of mitochondria.Brain Research 03/2008; 1196:121-30. · 2.83 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Astrocytic glutamate transporters are considered an important target for neuroprotective therapies as the function of these transporters is abnormal in stroke and other neurological disorders associated with excitotoxicity. Recently, Rothstein et al., [Rothstein JD, Patel S, Regan MR, Haenggeli C, Huang YH, Bergles DE, Jin L, Dykes Hoberg M, Vidensky S, Chung DS, Toan SV, Bruijn LI, Su ZZ, Gupta P, Fisher PB (2005) Beta-lactam antibiotics offer neuroprotection by increasing glutamate transporter expression. Nature 433:73-77] reported that beta-lactam antibiotics (including ceftriaxone, which easily crosses the blood-brain barrier) increase glutamate transporter 1 (GLT-1) expression and reduce cell death resulting from oxygen-glucose deprivation (OGD) in dissociated embryonic cortical cultures. To determine whether a similar neuroprotective mechanism operates in more mature neurons, which show a different pattern of response to ischemia than primary cultures, we exposed acute hippocampal slices obtained from rats treated with ceftriaxone for 5 days (200 mg/kg; i.p.) to OGD. Whole-cell patch clamp recording of glutamate-induced N-methyl-d-aspartate (NMDA) currents from CA1 pyramidal neurons showed a larger potentiation of these currents after application of 15 microM dl-threo-beta-benzyloxyaspartic acid (TBOA; a potent blocker of glutamate transporters) in ceftriaxone-injected animals than in untreated animals, indicating increased glutamate transporter activity. Western blot analysis did not reveal GLT-1 upregulation in the hippocampus. Delay to OGD-induced hypoxic spreading depression (HSD) recorded in slices obtained from ceftriaxone-treated rats was longer (6.3+/-0.2 vs. 5.2+/-0.2 min; P<0.001) than that in the control group, demonstrating a neuroprotective action of the antibiotic in this model. The effect of ceftriaxone was also tested in organotypic hippocampal slices obtained from P7-9 rats (>14 days in vitro). OGD or glutamate (3.5-5.0 mM) damaged CA1 pyramidal neurons as assessed by propidium iodide (PI) fluorescence. Similar damage was observed after pre-treatment with ceftriaxone (10-200 microM; 5 days) and ceftriaxone exposure did not result in GLT-1 upregulation as assayed by Western blot. Treatment of slice cultures with dibutyryl cAMP (100-250 microM; 5 days) increased GLT-1 expression but did not reduce cell damage induced by OGD or glutamate. Thus we confirm the neuroprotective effect of antibiotic exposure on OGD-induced injury, but suggest that this action is related to independent modulation of transporter activity rather than to the level of GLT-1 protein expression. In addition, our results indicate that the protective effects of beta-lactam antibiotics are highly dependent on the experimental model.Neuroscience 06/2007; 146(2):617-29. · 3.33 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: ATP is released during hypoxia from the ventrolateral medulla (VLM) and activates purinergic P2 receptors (P2Rs) at unknown loci to offset the secondary hypoxic depression of breathing. In this study, we used rhythmically active medullary slices from neonatal rat to map, in relation to anatomical and molecular markers of the pre-Bötzinger complex (preBötC) (a proposed site of rhythm generation), the effects of ATP on respiratory rhythm and identify the P2R subtypes responsible for these actions. Unilateral microinjections of ATP in a three-dimensional grid within the VLM revealed a "hotspot" where ATP (0.1 mM) evoked a rapid 2.2 +/- 0.1-fold increase in inspiratory frequency followed by a brief reduction to 0.83 +/- 0.02 of baseline. The hotspot was identified as the preBötC based on histology, overlap of injection sites with NK1R immunolabeling, and potentiation or inhibition of respiratory frequency by SP ([Sar9-Met(O2)11]-substance P) or DAMGO ([D-Ala2,N-MePhe4,Gly-ol5]-enkephalin), respectively. The relative potency of P2R agonists [2MeSADP (2-methylthioadenosine 5'-diphosphate) approximately = 2MeSATP (2-methylthioadenosine 5'-triphosphate) approximately = ATPgammas (adenosine 5'-[gamma-thio]triphosphate tetralithium salt) approximately = ATP > UTP approximately = alphabeta meATP (alpha,beta-methylene-adenosine 5'-triphosphate)] and attenuation of the ATP response by MRS2179 (2'-deoxy-N6-methyladenosine-3',5'-bisphosphate) (P2Y1 antagonist) indicate that the excitation is mediated by P2Y1Rs. The post-ATP inhibition, which was never observed in response to ATPgammas, is dependent on ATP hydrolysis. These data establish in neonatal rats that respiratory rhythm generating networks in the preBötC are exquisitely sensitive to P2Y1R activation, and suggest a role for P2Y1Rs in respiratory motor control, particularly in the P2R excitation of rhythm that occurs during hypoxia.Journal of Neuroscience 01/2007; 27(5):993-1005. · 6.75 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Glutamate excitotoxicity has been suggested to play a role in amyotrophic lateral sclerosis, since overstimulation of post-synaptic glutamate receptors by accumulated extracellular glutamate leads to motoneuron cell death. It is however unclear as to why some groups of motoneurons degenerate in this disease while other groups remain relatively intact even during terminal stages of the disease. Our previous studies in the rat showed differential expression of group I metabotropic glutamate receptors in motoneurons at low and high risk of degeneration in amyotrophic lateral sclerosis. Here we have extended this study to normal human brains. In situ hybridization showed that transcripts of both metabotropic glutamate receptor (mGluR) 1 and mGluR5 were expressed in motoneurons in both the resistant motor nucleus III and the vulnerable motor nucleus XII. Immunolabeling of mGluR1alpha and mGluR5 was found in both motoneurons and glia-like cells in all the motor nuclei and the ventral horn of the cervical spinal cord studied. Quantitative analysis of optical density measurements showed higher levels of mGluR1alpha protein expression but lower levels of mGluR5 protein expression in the vulnerable motoneuron pool (VII, XII and spinal cord) than in the resistant motoneuron pool (III, IV and VI). This differential expression of group I metabotropic glutamate receptor proteins within vulnerable motoneuron pools may predispose these neurons to degeneration as seen in amyotrophic lateral sclerosis.Neuroscience 12/2006; 143(1):95-104. · 3.33 Impact Factor
Publication Stats
3k | Citations | |
377.82 | Total Impact Points | |
Top Journals
University of Auckland
- • Department of Physiology
- • Faculty of Medical and Health Sciences
- • Department of Medicine
Окленд, Auckland, New Zealand -
Flinders University
- School of Medicine
Tarndarnya, South Australia, Australia
University of Alberta
- Department of Physiology
Edmonton, Alberta, Canada
Foundation Santa Lucia
Roma, Latium, Italy
The Nippon Dental University
- Department of Physiology
Tokyo, Tokyo-to, Japan
Murdoch University
- School of Veterinary and Life Sciences
Perth, Western Australia, Australia
Australian National University
Canberra, Australian Capital Territory, Australia
University of Birmingham
Birmingham, England, United Kingdom


text:
co-first authors
Population history in Arabidopsis halleri using multilocus analysis
Article first published online: 28 JUL 2010
DOI: 10.1111/j.1365-294X.2010.04761.x
© 2010 Blackwell Publishing Ltd
Additional Information
How to Cite
HEIDEL, A. J., RAMOS-ONSINS, S. E., WANG, W.-K., CHIANG, T.-Y. and MITCHELL-OLDS, T. (2010), Population history in Arabidopsis halleri using multilocus analysis. Molecular Ecology, 19: 3364–3379. doi: 10.1111/j.1365-294X.2010.04761.x
Publication History
- Issue published online: 5 AUG 2010
- Article first published online: 28 JUL 2010
- Received 17 September 2009; revision received 14 June 2010; accepted 15 June 2010
- Arabidopsis halleri;
- multilocus analysis;
- population genetics;
- population history
A. halleri is a psuedometallophyte with a patchy distribution in Europe and is often spread by human activity. To determine the population history and whether this history is consistent with potential human effects, we surveyed nucleotide variation using 24 loci from 12 individuals in a large A. halleri population. The means of total and silent nucleotide variation (θW) are within the range expected for the species. The population genetic neutrality tests Tajima's D and Wall's B had significant composite results rejecting panmixia, and Approximate Bayesian Computation analysis revealed that a subdivision model better explained the variation than the standard neutral model, refugia (or admixture), bottleneck or change of population size models. A categorical regression analysis further supports the subdivision model, and under the subdivision model, the neutrality tests are no longer significant. The best support was for two source populations, a situation consistent with the mixing of two populations possibly mediated by human activity. This scenario might limit the genetic diversity and adaptive potential of the population. The non-neutral population variation described here should be considered in bioinformatic searches for adaptation.

subset: commoncrawl


text:
Join us at the Disneyland Resort from May 25-27 for Opa! A Celebration of Greece, featuring music, dance, art, food, history and more! Festivities will take place in both it's a small world Mall in Disneyland park and Paradise Gardens in Disney California Adventure park. Mickey Mouse will even be there in his Fustanella attire!
Each day from 10 a.m.-6 p.m., these two areas will offer special experiences such as:
"it's a small world" Mall
- Olympics training with Hercules
- Atmosphere entertainment by "The Citizens of Greece"
- Live performances from Greek musicians and dancers
- Special Greek food offeringsParadise Garden
- Greek wine tastings
- Live performances from Greek musicians
- Kalamatianó line dances
- Special Greek food offerings
- Atmosphere entertainment by "The Citizens of Greece"And on May 26, you're invited to join Greek celebrity Chef Argiro Barbarigou and Greek winemaker Christina Boutari for a special dinner at the Golden Vine Winery in Disney California Adventure park. Reservations and tickets may be purchased online starting March 25, so check back for booking details.Come join us for Opa! A Celebration of Greece, May 25-27 at Disneyland and Disney California Adventure parks!
Wishing you Faith, Trust, and Pixie Dust
Related articles
- Visiting Cars Land at Disney's California Adventure (chipandco.com)
- Marvel Headed to Hong Kong Disneyland (chipandco.com)
Independent Travel Agent at World of Magic Travel Services
I am the Disney World News Writer for Chip and Company. I am also an Independent Travel Agent for World of Magic Travel, the preferred agency used by Chip and Company and the Disney Addicts. Email me at firstname.lastname@example.com so I can help you plan your magical Disney vacation.
Latest posts by Maria (see all)
- Disney World Swan and Dolphin Hotel Springtime Savings - February 28, 2015
- SeaWorld Orlando Kicks off Bands, Brew, and BBQ Festival - February 28, 2015
- Universal Follows Disney and Raises Ticket Prices - February 28, 2015
A Celebration of Greece Coming to Disneyland Resort May 25-27 was last modified: March 16th, 2013 by


text:
lrhr's Profile
Title | Last Reply |
What are some over-rated L.A. restaurants to you? Why, specifically? Only one each! I went to animal in it's very early opening days at least a half dozen times in a couple week span. Working on a play late night nearby with a large cast. Got told each time that the menu changes every day. No. It didn't. I was there yesterday and saw the exact same menu. And once I ordered the blueberry cobbler and got peach - which had been on the menu the week before... must have been sitting in the freezer. Some of our cast liked it and others were middling to dislike, but the only other late night choice was Cantor's. |
curing fish other than salmon? The very first time I cured salmon, I thought if salting it for 2-3 days is good; 3-4 must be better! Horrible and oversalted - but I had promised to bring it to a New Year's Eve party and anyplace I could buy a substitute was closed. I rinsed each sliced piece. And it wasn't bad. And people came back for seconds of mine while the storebought stuff just sat there. So, rinse away. (at this point, no loss, right?) |
Top Chef Boston – Ep. #5 – 11/12/14 (spoilers) Did anyone else find it strange that Mei just happened to have a box of instant dashi? |
Bad service (not horrible). Check adjusted. What to tip? Certainly, any *one* of these things is not a big deal. I mentioned it when the manager asked "how is everything?" and the list was long. Only a sample in my OP. |
Bad service (not horrible). Check adjusted. What to tip? The discussion happened prior to f-i-l grabbing the bill and treating. And m-i-l was equally vocal about wondering what was the etiquette here. The thread was... to find out what others thought should this happen elsewhere. |
Simon's Cafe in Sherman Oaks, a true find We like this place a lot. Everything is always good, but i particularly like the salmon. and the desserts are all made by simon, so what you get is what he's made. i can still taste the sour cherry tart .. just amazing. |
What really happens at a restaurant when the customer can't pay? Stopped at a cute breakfast spot on a roadtrip. Order at the counter and they bring the food sort of place. We put in the order, he stands waiting for his coffee while I grab a table. We are halfway back to the car when I say, "did you tip on the credit card or should we have left cash on the table?" He stops. Long silence. "I thought YOU paid." |
Bad service (not horrible). Check adjusted. What to tip? Ate at an upscale, if not quite fine dining restaurant with a party of four. There were a lot of small missteps that shouldn't happen at that price point (not knowing who ordered what, leaving the apps plates on the table til actually serving the entrees, handing food across the table, serving a beverage by reaching across another diner and almost putting a sleeve in their food, not clearing empty glasses....). Only the slow appearance of salads and oysters could have been at the kitchen end. |
Cachagua General Store - brunch? I ended up working the friday of the fourth of july holiday and the whole trip got cancelled. |
Cachagua General Store - brunch? This place sounds really interesting and worth a stop, but our schedule won't let us be anywhere near it on a monday night... but we could make sunday brunch. Two questions, please: Is there a menu (rather than a set meal) since we have some dietary restrictions? And is brunch as unique as dinner seems to be? I'd hate to go super far out of our way for an omelet. Even a really good omelet. Would love your feedback. Thanks! |
SloCo Pasty Co [San Luis Obispo] Thank you for the great recommendation for a place I would never have stumbled into! I got the veggie bake and my hubby got the chicken pot pie pasty driving through SLO on our driving home last day of vacation. Got them to go and ate them on the pier at Port San Luis. Still warm and really delicious. (the wine sauce "dip" is, perhaps, not ideal for eating on a bench without a table...but the drips were worth the mess.) |
Your preferred Burgundy brand for Beef Bourguignon and Coq Au Vin? The take away from the (fantastic) times article: all the hard edges of a cheap wine get softened by the cooking process. all the complexities and subtleties of an expensive wine get smoothed out. |
"moral" food preferences, as a guest I would say that I am a "vegetarian who cheats sometimes." And then ask them to treat you as a vegetarian. If they are good enough friends you explain when and why you choose to 'cheat'. After a long time of keeping a kosher home and eating out as a vegetarian, I too have expanded to poultry under certain circumstances that make me comfortable. My friends are used to seeing kosher chickens served at my home and my being a vegetarian in theirs. As long as you aren't asking them to provide you with kosher/organic more expensive meats, I don't see the problem with stating that you are still "basically" a vegetarian. |
Your Biggest Restaurant Menu Pet Peeve The World Series was originally sponsored by the World Newspaper. Paper folded. Series continues. |
Spargelkarte in LA? (Asparagus menu) Don't know your price point, but Melisse currently has an asparagus menu on it's website. Cheaper than flying to Germany. Barely. ($195) |
I was a big fan of chapa grill...but the valley location, at least, is no longer kosher. They had a sign saying their schwarma is still kosher...but the restaurant as a whole isn't any longer. I'm gonna seriously miss the rotisserie chicken. |
Passover product fails. Haddar mayonnaisse. Hmmmm...that is possible. I was stuck in a hotel for passover and the room fridge was colder than I realized. On a plus note, the Galil multi-berry preserves was good on a lot of levels. I'd buy it year-round. |
Passover product fails. Haddar mayonnaisse. I bought a squeeze bottle of Haddar mayonnaisse. It visually looked fine in the bottle, but the moment it hit the matzah, it turned back into oil. A matzah sandwich isn't all that great to start with, but this was just disgusting. (a choice between a completely dry sandwich and one with oil dripping through the holes of the matzah. fortunately, i had ripe avocados ready to replace the stuff) This was actually worse than the Manischevitz breakfast/energy bars I bought a number of years ago that tasted like bubble gum. Not sure what brands I've bought before, but I'll avoid Haddar in the future. |
Do try to be organized enough to do it the day before. Not only does it give you a chance to skim fat, but if you cook it, slice it and then let the slices sit in the liquid overnight and reheat in the liquid you will have moist brisket. Straight out of the oven, it is dry. (I cook mine with onions underneath and a wine/brown sugar/tomato paste/cider vinegar mixture poured over the top to cover. cover the pan with foil and bake 3 hours at 325. comes out distressingly dry when sliced. and then, presto, moist and falling apart for the next day.) |
Is it ever acceptable to give service instruction to a server? I think this is a great way to put it. It lets the server know that you understand you are asking for something to be done differently from the norm. And that is very different from telling them that you assume they will do it 'wrong'. Etiquette be damned, my mother is one of those who cannot stand having her empty plate in front of her once she is done eating. I prefer it as well, but it's not a big deal to me...though it is nice having room to put my elbows on the table. |
Is There Such a Thing as an Openly Gay Friendly Chinese Restaurant in Chinatown/SGV? While I'm sure that there is likely to be some overlap of vegetarians, my point was that I could only attest to other people appearing to like the food. I ate mostly steamed broccoli. Which is fine. I don't expect my dietary choices to be catered to (especially if i'm not paying) as long as I don't actually starve. I didn't eat the food so I don't have an opinion on it. Simply that, as a giant corporate entity, the Hilton may be in a better position to meet the OPs policy requirement and that they put on what *looked* like an authentic banquet which appeared to be enjoyed by a mostly chinese-speaking crowd. |
The Modern by the window in winter? Thank you! |
The Modern by the window in winter? Hello, Since the dinner will be early March, does anyone know how warm those tables might be? Out here in LA where we don't have winters, on a cold (for us) night, the window seats often have a chill from the glass. I'd assume double paned glass and all that in NY, but don't want to be wrong and cold. And I am quite the cold wimp. I'm prepared to dress for the cold outside...but not on date night. And...while I'm asking...anyone know about their willingness to cater to dietary restrictions? Thanks. |
Is There Such a Thing as an Openly Gay Friendly Chinese Restaurant in Chinatown/SGV? Agree as to the Hilton. Went to a Hong Kong Merchants Association Banquet (or something) there with the vast majority of guests appearing to be Chinese and/or Chinese American. Everyone seemed to enjoy the food. I cannot speak to it as it was very far from vegetarian friendly (something to discuss if you end up there). I dined on steamed brocolli. |
Kitchen Misunderstandings with the Significant Other if only i were willing to go to the gym every single day for several hours of cardio, i would have him make those potatoes every night. |
Kitchen Misunderstandings with the Significant Other I made mashed potatoes occasionally and figured DH had seen enough to know the basic recipe. I asked him to finish prepping them one night while I did other tasks. He drained them (two medium sized), mashed them, added dairy and salt. I take a bite, and holy cow, they are the best mashed potatoes I have ever had. And the richest. By far the richest and creamiest mashed potatoes I have ever had. "how much butter did you put in here?" |
Recommendations for East Village This should have posted following Kathryn's link and ended up nowhere near it. Can't figure that out. Kathryn.....Wouldn't it have been faster to list the places that are NOT worth a visit?! Holy cow! |
Please help me save my too salty, home cured salmon. The first time I home cured salmon, it was way too salty (i thought, if brining it for two days was good...three or four would be better). I sliced it, tasted it and ack. I rinsed each piece under running water, not for too long...tasting as I went. (if you have lots of bits, rather than beautiful slices...put them in a sieve. i still get slices and bits and shards of salmon when i cut it up). Still saltier than I'd have liked (but not too much so), the texture was fine...though different than lox (which gets smoked in addition to the brining) I thought I'd failed entirely, but didn't have time to make or buy a substitute. Took it to a New Year's Breakfast party, put it down next to the store bought. Mine was gone, the store bought was not. Got lots of compliments. |
What would you take from abandoned pan-Asian restaurant? In california, at least, the items still belong to restaurant owner. They have a certain amount of time to collect them, and if they don't, assuming that the value is over a certain amount (either 100 or 1000) the landlord is responsible for auctioning off the items and using the money to repay any amount of default (and the cost of the auction) with the extra money going back to the ex-tenant. |
Help with 'Old' Hollywood Dining itinerary + questions re. Cicada Just to add to the traffic issue. That weekend is Carmaggedon 2. The 405 will be closed in both directions through the sepulveda pass. You aren't crossing into the valley at all, but spill over traffic might still add to your times. For the first Carmaggedon, lots of people stayed home and driving was actually smoother than usual. Could happen again, or you could have lots of people heading out confident that "everyone else" is staying home like last time. |


text:
Wow! That's some really vintage fragrance, there. Ours doesn't last more than five minutes or so.
Bonus: Get a load of the out click. That's where I refer my flatulent readers.
April 08, 2008
Visitor of the day -- 4/8
Posted by Attila at 10:38 PM
Subscribe to:
Comment Feed (RSS)


text:
Pronunciation: mrtvo
Added on: 2012-12-25
Listened: 9 times
Add categories to mrtvo
Pronunciation in Slovenian [sl]
- Pronunciation by whitelion (Female from Slovenia)
- Could you do it better? different accent? Pronounce mrtvo in Slovenian
Accents & languages on maps
- Random word: sveže
Not satisfied?
Modify word:
· Add languages
· Add categories
Report word:
Is there anything wrong with this word?
Please report it here.


text:
Just make one call and we'll get it done.
MON 8:00am - 5:00pm | TUE 8:00am - 5:00pm | WED 8:00am - 5:00pm | THURS 8:00am - 5:00pm | FRI 8:00am - 5:00pm | SAT closed | SUN closed |
Welcome to Custom Additions & Carpentry Services, Inc.!
Custom Additions & Carpentry Services, Inc. is a remodeling company serving customers in Omaha, Nebraska and the surrounding areas. Our full-service remodeling company has provided our customers with innovative remodeling services and products for many years. Our professional contractors use high-end materials for your home improvement needs.
Are you looking to make quite a few upgrades around your house? We do remodeling for home additions, kitchens, bathrooms, and more. Our team of general contractors and remodeling experts provides customers with affordable home improvement services.
Our remodeling specialties include:
• Additions
• Remodeling: Basement, Kitchen and Bathroom
• Cabinets
• Flooring
• Restoration
• Tiling
Additionally, we offer handyman services and carpentry to our customers. We are your total renovation company offering customers quality services. Professionally resolving your remodeling needs is our main goal. Just make one call and we'll get it done. This allows you to spend your time doing what you really want.
Call us today for more information about our remodeling services. Please visit our website for more information.
Just make one call and we'll get it done. Spend your time doing what really matters.
Category: Room Ideas|Home Additions|Bathroom Ideas|Cabinets|Plumbers|Residential Remodeling Contractors|Additional Home Services|Remodeling Contractors|Electricians|Basement Ideas|Handyman Services|Residential Handyman Services|Home Restoration|Home Projects
DexKnows Mobile profile for Custom Additions & Carpentry Services, Inc. in


text:
Naming the socially minded Japanese architect as the 2014 Pritzker laureate doesn't quite let the jury off the hook for snubbing Denise Scott Brown last year—but it's a start.
When truth is stranger than science fiction, architecture that draws from sci-fi starts to look downright normal.
Edward Snowden was a toddler when Elizabeth Diller and Ricardo Scofidio began to explore the inverse relationship between security and privacy. Now their work seems prescient.
Bertrand Goldberg's Prentice Memorial Hospital is being torn down, and the property's owner, Northwestern University, is continuing its assault on architecture.
The U.S. government has technology that can convert human waste into liquid fuel. So why are we still relying on petroleum imported from the Middle East?
Marshall Berman recently passed away, and Joseph Rykwert has just won the RIBA Gold Medal. Which makes me wonder: What ever happened to architecture theory?
Or, how I spent my summer vacation.
Artist Walter De Maria, who died last month at the age of 77, leaves us with powerful lessons about the virtues of hard-won simplicity.
While the city of Detroit stares bankruptcy in the face, its central business district is enjoying a renaissance.
Harry Weese's D.C. Metro stations are in danger of insensitive renovation. If changes really are necessary, don't these modern landmarks deserve the best?
Check out these stories, which take a close look at innovative projects that are using wood in creative ways. Proudly sponsored by reThink Wood.


text:
Expedition & Alpine forumAlpine & world mountaineering | |||
View latest messages in all forums You must Register as a New User before starting a new thread in this forum. Planning an overseas expedition or your first trip to the Alps? Route and area info. Travel, logistics, bureaucracy, equipment, etc.
Ailsa Graham is 20 years old and from Nottingham. Two years ago she was diagnosed with Asperger Syndrome. In this article... Read more
Buy an Edelrid Rope and get a free Edelrid Liner Rope Bag (MSRP £25) OR Buy a Sterling Rope and get a free... Read more
If you've been keeping an eye on the latest developments in adventure film over the last six months, you'll have been... Read more
N amed after one of the most famous ice climbs on The Ben, the Point Five NG wears its intended purpose on its sleeve. This... Read more
Tonight's Friday Night Video features Austrian climber Hansjörg Auer making the first ascent of a route on... Read more


text:
Magic sign Dwayne Dedmon for remainder of season
MAR 17, 2014 2:44p ET
ORLANDO, Fla. (AP) The Magic have signed center Dewayne Dedmon for the remainder of the regular season.
Magic general manager Rob Hennigan announced the signing Monday.
Dedmon, who signed the first of two 10-day contracts on Feb. 25, has appeared in three games with Orlando averaging 2.3 points and 1.0 rebound per game.
Undrafted in 2013, the 7-footer played 18 games this season with the Warriors and 76ers. He also appeared in 15 games with the NBA Development League's Santa Cruz Warriors.
A second 10-contract for swingman Adonis Thomas has expired. He appeared in four games with the Magic.


text:
See items selected especially for you. Shop recommendations.
Exclusive finds with a new theme each month.
Shop: Pop-In
Our director of creative projects shares what she's into.
Read More
Stay updated with a steady stream of new info.
An exquisite appliqué overlay fronts a classic shift dress accented by an elegantly scalloped hemline.
Watch the video: Dress Fit Fundamentals
Learn More: The Best Dress for Your Body Type


text:
You're not too far away from accessing comprehensive information on Hector Jiminez in Bloomington IL. Below, you'll find people search results for everyone with the name as Hector Jiminez who lives in Bloomington IL. Check-out personal information including email, age, phone number, and aliases.
Discover the Hector Jiminez in Bloomington, IL you're in search of right away, as all of our people search results are displayed systematically under four separate headings - name/aliases, age, location, and possible relations. Give the search results a once-over and find the Hector Jiminez you want who lives or once lived in Bloomington, IL. To get more details, click on the Details link.
Enhance your search results for Hector Jiminez in Bloomington by keying in additional details like Hector's middle name, age, and address. Once you've found the Hector Jiminez you want, you can view Hector Jiminez's contact info and more by joining us. At USA-People-Search.com, you can choose from up to three different types of memberships, each of which offers its own set of benefits and tailored to meet your specific need.


text:
Groninger Museum - Artworks
Displaying 1 through 6 of 6 works, sorted by
, in ascending order.
Athenaeum forums
Groninger Museum
Choose a sort
By artist name (ascending)
By artist name (descending)
By medium (ascending)
By medium (descending)
By title (ascending)
By title (descending)
By upload date (ascending)
By upload date (descending)
By year completed (ascending)
By year completed (descending)
Drawbridge in Nieuw-Amsterdam
Vincent van Gogh (1883)
Painting - watercolor
Uploaded Monday, 1 May 2006 by member rocsdad
Public domain
Jan Albert Sichterman with his son Jan Albert
Philip van Dijk (1745)
Uploaded Saturday, 2 February 2013 by member Irene
Public domain
Man in a Helmet
Carel Fabritius
Painting - oil on panel
Uploaded Wednesday, 7 January 2015 by member kohn1fox
Public domain
Added 57 days ago.
My Studio
Sir Lawrence Alma-Tadema (1867)
Painting - oil on panel
Uploaded Thursday, 26 December 2002 by member rocsdad
Public domain
Sybilla Volkera Sichterman-Sadelijn and her daughters Sybilla Volkera and Christina Elisabeth
Philip van Dijk (1745)
Uploaded Saturday, 2 February 2013 by member Irene
Public domain
Vaugirard Church by Night
Paul Gauguin (1881)
Painting - oil on canvas
Uploaded Wednesday, 1 January 2003 by member rocsdad
Public domain


text:
- 284Promote
Art Print
Collect your choice of gallery quality Giclée, or fine art prints custom trimmed by hand in a variety of sizes with a white border for framing.
Thanks so much for the lovely comments Malin, Vitta, Rich, Gale, Tomas, Layton, crocomila, Nick, david Pame and Peter! ^_^
See More Comments


text:
450 Marion, MA Tile, Stone & Countertop Manufacturers and Showrooms
Tiles Plus More Incorporated
(508) 653-4252
Here at Tiles Plus More we strive to provide our customers with the best service and satisfaction possible. We currently have 15 associates on our staff including both full and... Read More
Stone Seal
(508) 406-5350
Stone Seal specializes in the restoration, repair, and maintenance of natural stone countertops, floors and walls. Grinding, honing, polishing, leathering, cleaning, and sealing... Read More
CB Tile INC
DM Granite, Marble and Tile Inc.
(508) 460-9032
DM Marble & Tile began as a local stone cutting and installation business in East Marlborough, MA. As we started to improve and establish a reputation for high-quality services,... Read More
Modern Boston Tile, Inc
(617) 982-2133
Premier Stone
(508) 921-4499
Premier Stone is an importer and distributor of natural stone and tile. We work closely with the best fabricators in the area to find the proper stone for all applications. Read More
M S International, Inc.
(781) 680-0300
MSI Boston features a newly constructed 4,000 sq. ft. state of the art showroom for flooring and wall tile and a separate 15,000 sq. ft. Full Address:1080 University... Read More
Olympia Marble & Granite, Inc.
(781) 455-0199
Offering the finest in natural & engineered stone since 1984, Olympia Marble & Granite, Inc. is a premier showroom and fabrication house. Read More
Atlantic Marble and Granite
(508) 540-9770
We are fabricators for all your stone needs. we do all of our own templates,fabrication & installation of kitchen counters,vanity tops,fireplaces, tubdecks,custom furniture & more Read More
(508) 985-8074
We are tile , stone and glass installers. We have been in business for over 25years
We install all types of flooring and specialize in custom bathrooms and kitchens from frame... Read More
power crete
(508) 443-1797
Decorative concrete business dealing in concrete flooring, counters, surrounds, planters, fire pits, and more. Read More
Garcia Imported Tile & Granite
Martha's Vineyard Tile Co. Inc
(508) 693-9707
Martha's Vineyard's only full service tile and stone showroom. Read More
List your business here for free - Learn More


text:
Renvyle, Renvyle, Ireland – Show map
Excellent location – rated 9.6/10! (score from 55 reviews)
Rated by guests after their stay at Renvyle House Hotel.
Food and scenery
United Kingdom
Liked everything about the place, including location, comfort and very helpful friendly staff
Location was lovely, just walk out the door and you were on a beautiful beach with all amenties just a stones throw away.
We had a quite room. The location is beautiful.
Smell of turf fire in the lobby Music in the bar
Location is stunning Good food
Nothing was too much trouble for staff- Heather was even able to visit in the kitchen to see wheaten bread being made.
Situation Sea side and also lake side Hospitality Quality of food
Restaurant, outdoor facilities and location.
The area is beautiful and peaceful
Surrounded by private parkland on the shores of the Atlantic, Renvyle House features a golf course, swimming pool and award-winning restaurant. With cozy turf fires, there is also free parking and free Wi-Fi.
With views of Twelve Bens Mountains and Rusheenduff Lake, the colorful bedrooms feature private bathrooms, tea and coffee facilities and satellite TV.
The Renvyle Restaurant serves a traditional Irish menu, featuring local produce and fresh fish. There is also a cozy bar and guest lounge, offering a selection of beverages and whiskeys.
The 200 acres of private grounds include sandy beaches, lakes, scenic woodlands, a 9-hole golf course and tennis courts. There is also an outdoor swimming pool, croquet lawn and clay pigeon shooting facilities.
Set in a romantic period house, The Renvyle Hotel is located on the Connemara Loop. Surrounded by rugged coast and historic countryside, Connemara National Park is just a 10-minute drive away.
We speak your language! Hotel Rooms: 70 This property has been on Booking.com since May 3, 2008.
Low rates
No booking fees • Save money! Best price guarantee — enter dates to see these prices!
10 properties in Renvyle
Including hotels, apartments, villas and more.
53 verified reviews
We verify all reviews from guests who have stayed at Renvyle House Hotel. Find out more.
They speak your language!
Renvyle House Hotel's staff speaks: English
Booking is safe
When you book with us your details are protected by a secure connection.
Outdoor Pool (seasonal), Grounds, Terrace, Sun Deck
Tennis Court, Golf Course (within 2 miles), Fishing, Pool Table, Ping-Pong, Canoeing, Hiking, Cycling, Bowling, Diving, Mini Golf, Water Sports Facilities (on site), Sauna, Massage, Hot Tub, Private Beach Area, Library, Playground
Food & Drink
Restaurant, Bar, Breakfast in the Room
WiFi is available in public areas and is free of charge.
Free private parking is available on site (reservation is not needed).
Room Service, Packed Lunches, 24-Hour Front Desk, Baggage Storage, Babysitting/Child Services, Laundry, Ironing Service, Meeting/Banquet Facilities, Fax/Photocopying, Honeymoon Suite
Newspapers, Safe, Non-smoking Rooms, Facilities for Disabled Guests, Family Rooms, Heating
Cancellation and prepayment policies vary according to room type. Please enter the dates of your stay and check what conditions apply to your preferred room.
Children and Extra Beds
All children are welcome.
All children under 2 years stay free of charge when using existing beds.
All children under 2 years stay free of charge in a crib.
All children under 14 years are charged EUR 20 per stay when using existing beds.
The maximum number of extra beds/cribs in a room is 1.
Additional fees are not calculated automatically in the total cost and will have to be paid for separately during your stay.
Pets are allowed. Charges may apply.
Cards accepted at this property
Hover over the cards for more info.
Other Places you Might Like
Based on 55 reviews
Value for money
Free WiFi
Show reviews from:
Sort by:
Fuchsia House Bed and Breakfast Connemara
Latest Booking: March 4
Featuring modern rooms with 22-inch Plasma-screen TVs, Seafield House offers beautiful Atlantic views. Guests can enjoy an extensive and hearty breakfast menu, including homemade bread and pancakes.
Score from 147 reviews
In magical Connemara, Ireland's most beautiful location, this charming, contemporary seaside hotel with 'olde world' charm offers luxury accommodations, excellent cuisine, caring service and warm,...
Score from 102 reviews
Sea Breeze B&B is a family-run bed and breakfast located in the heart of the Connemara district of County Galway.
Score from 78 reviews
On the shores of Killary Harbour lies Ireland's oldest coaching inn. Dating from 1796, you'll find traditional home cooking, antique furniture and the most spectacular views in Ireland.
Score from 365 reviews
Our leisure visit to Renvyle House Hotel was a delight! The staff, reception, restaurant and cleaning staff, were all very helpful, efficient and friendly. The location is superb, with the Atlantic and three sides of the hotel and a lake at the front. The rooms were clean, spacious and comfortable. The breakfast had everything that you could wish for. Dinner was a delight and served with efficiency and charm. Altogether a most memorable visit and one that will certainly be repeated.
Nothing - a wonderful stay from the moment we arrived until, with regret, we departed!
We liked the house,the comfort, the food, the house surrounds,Renvyle Beach,the cultural connections, Yeats, Oliver St.John Gogarty,Augustus John, Lady Gregory,the wonderful production on Monday evening by 'The Curlew Theatre Company,the peace,calm,tranquility.Just what we needed after long months of hard work.We are very grateful. Thank you all.
All the facilities. The food. The friendliness of the staff. The view of the sea from the bedroom.
No biscuits in the room for our tea. I would like cups instead of mugs for tea in our rooms as we have mugs at home.
The staff were extremely friendly.When we arrived in our room, once we turned on the lights, the sockets blew.We asked the receptio nist for a sidelamp as we were only staying for one night and did not mind staying in the same room.Instead, we were upgraded to a fabulous room.so unexpected.The food was amazing in the restaurant also.We loved our stay here.fabulous location and grounds.Would stay here again
very dirty rooms full of dust. double bed was sunk in the centre. not a nice thing to look at the moment you enter the room. the room key was attached to a big dirty wooden block. staff were very friendly and helpful but the hotel charge way too much for what you get. i would class this hotel lower than a hostel
Liked everything about the place, including location, comfort and very helpful friendly staff
Location was lovely, just walk out the door and you were on a beautiful beach with all amenties just a stones throw away.
Bar food finished at 8pm and there was no chicken or steak on main restaurant menu. Not everybody likes fish/shellfish.......
We had a quite room. The location is beautiful.
The hotel is way too big, it is unpersonal, too expensive and cannot cater for individual requests, despite offering them through the booking portal (i.e. single beds or double bed). The food is simply a disgrace and ranges among the worst ever consumed in Ireland.
Smell of turf fire in the lobby Music in the bar
Location is stunning Good food
Too many kids running around the place at night
Nothing was too much trouble for staff- Heather was even able to visit in the kitchen to see wheaten bread being made.
Nothing even 50 years after we first went there.
Situation Sea side and also lake side Hospitality Quality of food
Restaurant, outdoor facilities and location.
The room was completely airless and suffocating in warm weather. No aircon. Had big window but only a tiny section of it opened, letting in very little air. TV set was almost unusable and last century's technology.
The area is beautiful and peaceful
No 6 . the bed was not comfortable. Well we paid 190 euro for b and b and it was just about worth 90euro.The hotel needs a make over if you are to continue to charge these prices. Its dated and those beds need replacing
Tranquility is the word that comes to mind.
A bit inconvenient for older generation.
Loved the incredible grounds, relaxed atmosphere and very friendly staff!
The location, wonderful setting, great sunset, beautiful gardens, excellent food and most of the staff very friendly.
Reception person not enthusiastic or particularly helpful on first morning when we tried to find out where there were good walks in the grounds and nearby. We were at the end of our 48 hr stay before we discovered all the facilities the hotel had. We thought the room was rather basic for 149 euros per night!
Fantastic meal at a really good price. Relaxing location.
Single bed bit small and creaky
The friendliness and helpfulness of the staff. The facilities available at the hotel and also the surrounding area. Shopping in Clifden. The scenery. THE FOOD- yum,yum,yum.
Meal in restaurant very good. Service very good, place very clean.
Whole place bit dated, Restaurant old fashioned 70s style. Rooms like wise,It is what it says 3* hotel in lovely location. No more no less..
Location very relaxing and picturesque. Accommodation comfortable and peaceful. Lovely older section with cosy lounges. Interesting gardens and right on beach. Breakfast offered good variety and excellent service from staff.
Casual dinner would have been better at proper tables rather than coffee tables.
Most Popular Cities


text:
Top Apps
- Audio & Video
- Business & Enterprise
- Communications
- Development
- Home & Education
- Games
- Graphics
- Science & Engineering
- Security & Utilities
- System Administration
WordPress Form Maker(Builder) plugin Form Maker
9 weekly downloads -
WordPress event calendar plugin WordPress event calendar plugin
3 weekly downloads -
BIONA Project BIONA is a project framework for Bulgarian small business. This framework is based on open source and QT3 (GPL licence) and include modules for Accounting (General Ladger, AP, AR, VAT, and etc.), Store, POS, Clients, Vendors, Billing, Generator for Report
2 weekly downloads -
MedSurvey A medical information system for Windows-based systems (95-XP/CE/PocketPC) based on Open Source platforms
1 weekly downloads -
IT Exam Practice Webapp ItExamPractice is a .NET Web application for sites offering online exams/tests. Works in MS.NET and a free (Linux/UNIX/Mono) environment. It helps communities collaboratively build exams and guarantee the freedom of the information for use by others.
0 weekly downloads -
Paracelsus A Medical Information System (MIS) Application Server based on Web Services and J2EE and administrative tools (Plugins for Eclipse,...)
0 weekly downloads


text:
A friendly place for programming greenhorns!
Big Moose Saloon
Java FAQ
Recent Topics
Flagged Topics
Hot Topics
Zero Replies
Register / Login
Win a copy of
Elasticsearch in Action
this week in the
Big Data
Java Forums
Page fowards
sumanth kadaba
Joined: Jun 21, 2009
Posts: 27
Oct 14, 2009 15:33:33
Hi All,
I have a website which contains a header, footer, navigation on the left and a content area. So this is the design part of the website.
The content area is filled up in run time which will be including different pages.
Recently I had to work with MQ and the application had to communicate with the MQ. So I proceeded forward and everything was successful.
However I began to notice that whenever there has to be a communication with the MQ, it takes considerably longer time for the output to be shown in the GUI.
So this creates a longer response time.
So I decided to show the user a waiting message on the screen while the processing took place, but it dint go the way I thought. I used client side javascript for this.
So the problem in brief:
I have a page in which I can do some selections, on submission I am doing some processing on MQ and then forwarding the user to a different page. This operation takes place for a long time.
So Can I have a temporary waiting page which can be shown to the user... and then show the actual page to the user after the processing.
This would mean I would have to find some way to have two page forwards from the action.
I would like to have some idea to be able to do this or anything similar.
Thanks in advance
I agree. Here's the link:
subject: Page fowards
Similar Threads
Resetting response in action class
Avoiding the page to get refreshed
HOW: 'if'-like JSF conditions?
how to show a message while processing is going on
Decision based on priority
All times are in JavaRanch time: GMT-6 in summer, GMT-7 in winter


text:
Narrow your search
Lynn La is CNET's associate editor for cell phone and smartphone news and reviews. Prior to coming to CNET, she wrote for the Sacramento Bee and was a staff editor at Macworld. In addition to covering technology, she has reported on health, science, and politics.
Unveiled at MWC 2015, the BlackBerry Leap runs the new 10.3.1 OS, and is equipped with a 5-inch HD display and 8-megapixel camera.
At MWC 2015, Ford took the wraps off its MoDe:Pro and MoDe:Me electric bike prototypes, which connect to the Apple iPhone 6.
Sporting a colored e-paper display, a microphone and a slimmer profile, Pebble's new Time smartwatch is initially available only on Kickstarter.
The Nubia Z9 Max from ZTE features a 16-megapixel camera, a dual glass-panel body, and Android 5.0 Lollipop.
The polished Nubia Z9 Max from ZTE features a 5.5-inch display, a 16-megapixel camera, and loads of camera features.
Designed by Google and manufactured by Yezz, mock-ups of the highly anticipated Project Ara modular phone show up at MWC 2015.
During a press conference at MWC, car company Ford introduced its MoDe:Pro and MoDe:Me concept e-bikes.
At MWC 2015, Alcatel showcases the 7- and 8-inch variants of its OneTouch Pixi 3 Android tablets.
With 4G+, 4G, and 3G models, these Wi-Fi Link hotspots from Alcatel keep you connected.
The compact MD health monitor from B-ON lets you view and collect your heart rate and blood pressure data, and saves it all on an app.
Pricing not available


text:
With the right policies and investments in public infrastructure and services, Bulgaria could achieve its goal of converging with Europe in the next 20 years, according to the World Bank.
Experts believe that Belgrade would be more attractive to foreign investors if corruption is reduced.
Investments in agriculture and energy will be especially important for economic growth.
As terrorist attack threats become more frequent globally, Macedonia and the region employ anti-terrorist safety measures.
The decision to construct a new nuclear power plant will be put to a rare referendum.
Russia's Gazprom will reduce gas prices by 20 percent as part of the agreement. Also in business news: Turkey officially introduced the prototype of the first national tank and Greek Prime Minister Antonis Samaras is embarking on an investment tour next week.
By Katica Djurovic and Bedrana Kaletovic for Southeast European Times in Belgrade and Sarajevo -- 26/11/12
Croatia's campaign against domestic violence targets young students, teens, parents and the community at large.


text:
Grow Your Own Food in March!
- Home & Lifestyle
- Garden for the Environment, San Francisco CA
Intro to Rainwater HarvestingGARDEN FOR THE ENVIRONMENTSaturday, November 16, 2013 from 10:00 AM to 12:00 PM (PST) |
Date: Saturday, November 16, 2013
Time: 10am - 12noon
Location: Garden for the Environment, 7th Ave at Lawton Street, San Francisco
Instructor: Tara Hui, Instructor with Greywater Action
Cost: Free! Sponsored by the SFPUC
Interested in rainwater harvesting? Thinking of setting-up a rainwater harvesting system? Now is the perfect time to start planning in preparation for our fall and winter rains. Join Tara Hui, Instructor with Greywater Action for this free, hands-on class sponsored by the SFPUC. You'll learn what to consider and where to start for systems as simple as a single barrel under a downspout, or as complicated as an indoor plumbing system for toilet flushing.
In order to purchase these tickets in installments, you'll need an Eventbrite account. Log in or sign up for a free account to continue.


text:
Fleurus 1 Star Hotels - Hotels.com offers a large selection of 1 Star hotels in Fleurus at great discount prices. Search for 1 Star rooms in Fleurus and compare room rates to suite your needs. Rest assured, no matter where you're travelling to, hotels.com has a great deal for you.
1 star Fleurus hotels
Popular with travelers from US
One Star hotels in Fleurus
ibis budget Charleroi Aéroport
Good 3.8 / 5 ( 183 reviews )
75 people looked at this hotel in the last hour
Most recent review
"Room was clean but absolutely no frills. Free parking and good location to airport."
A Traveller, Dec 2014, GB
One Star hotels within 25 miles of Fleurus
Hotel Studio Shelter
Fair 2.9 / 5 ( 13 reviews )
38 people looked at this hotel in the last hour
Splendid Palace Dinant Hostel
No Guest Reviews yet
10 people looked at this hotel in the last hour


text:
By Sport
By Columnist
- Selected:
Jerry Crasnick - All Columnists
- Selected:
By Insider
28 results for "baseball hall of fame"
Randy Johnson opts for Arizona Diamondbacks logo on Hall of Fame ...
Jerry CrasnickRandy Johnson became a major league star with the Seattle Mariners, but he'll enter the Baseball Hall of Fame representing the Arizona Diamondbacks.
Story | Conversation | January 16, 2015 -
Hall of Fame pitchers push for more to join them in Cooperstown
Jerry CrasnickWith five pitchers joining the Hall of Fame in the past two years, will more follow in the years ahead?
Story | Conversation | January 07, 2015 -
Randy Johnson endorses former Seattle Mariners teammate Edgar Mar...
Jerry CrasnickIf high-profile celebrity endorsements can help sway the electorate, Edgar Martinez might have gained some traction this winter in his case for a spot in Cooperstown.
Story | Conversation | January 07, 2015 -
Allen, Oliva fall short of Hall
Jerry CrasnickSAN DIEGO -- A chatty and energetic former Philadelphia Phillies groundskeeper named Mark "Froggy" Carfagno spent months on a nonstop crusade to get Dick Allen elected to the Baseball Hall of Fame. So it came as a gut punch Monday morning when Jane F...
Blog | December 08, 2014 -
MLB - Pete Rose still a walking contradiction 25 years after ban
Jerry CrasnickOn Aug. 24, 1989, A. Bartlett Giamatti banished Pete Rose from baseball for life. A quarter-century later, an inside look at Rose's days, weeks and months leading up to that moment, and at Pete's life now.
Story | Conversation | August 21, 2014 -
MLB -- Six legends welcomed into Hall of Fame
Jerry CrasnickSix legends joined the ranks of the Baseball Hall of Fame on Sunday. Jerry Crasnick breaks down the 2014 induction ceremony.
Story | Conversation | July 27, 2014 -
Atlanta Braves' star power will be on display at Hall of Fame
Jerry CrasnickGreg Maddux, Tom Glavine and Bobby Cox made their mark over a decade and a half with class and a penchant for winning baseball games.
Story | Conversation | July 25, 2014 -
Bobby Cox, Tony La Russa and Joe Torre get the Cooperstown call
Jerry CrasnickLegendary managers Bobby Cox, Joe Torre and Tony La Russa will be inducted into the Hall of Fame together, and rightfully so.
Story | Conversation | December 09, 2013 -
Ozzie Smith selling memorabilia at auction
Jerry CrasnickHall of Famer and former St. Louis Cardinals great Ozzie Smith is selling his 13 Gold Gloves, 11 of his All-Star Game rings and more than 100 pieces of memorabilia.
Story | Conversation | October 16, 2012 -
Sandberg's baffling managerial wait
Jerry CrasnickALLENTOWN, Pa. -- Ryne Sandberg takes a brief hiatus from his managerial duties each July and heads to Cooperstown, N.Y., for the Baseball Hall of Fame induction ceremonies. It's a nice opportunity to play some golf, relive old memories and enjoy the...
Blog | September 02, 2012 -
Ryne Sandberg still waits for elusive big league manager job
Jerry CrasnickRyne Sandberg has been in his minor league managing limbo for six seasons now. His shot at a major league job is overdue.
Story | Conversation | August 31, 2012 -
Roberto Alomar, Bert Blyleven voted into Hall of Fame, but steroi...
Jerry CrasnickRoberto Alomar and Bert Blyleven were voted into the Hall of Fame, and they deserve induction, but what was equally clear after the ballots were counted was that steroids cloud now firmly hovers over Cooperstown.
Story | Conversation | January 05, 2011 -
Hank Aaron says he thinks those linked with steroids should have ...
Jerry CrasnickFormer home run king Hank Aaron said Saturday that he's willing to accept steroid users into the club, but with asterisks.
Story | Conversation | July 25, 2009 -
Unyielding, gruff Williams was an innovator
Jerry CrasnickThere's a lot more to Dick Williams than his legendarily cantankerous clubhouse presence might suggest.
Story | Conversation | July 25, 2008 -
Tanner backing Gossage, Blyleven
Jerry CrasnickFormer manager Chuck Tanner is happy to give Goose Gossage and Bert Blyleven both his blessing and his personal endorsement for the Hall of Fame.
Story | Conversation | December 27, 2007 -
Ripken Jr. endorses Rice, Gossage for HOF; Gwynn speaks up for Da...
Jerry CrasnickOne fringe benefit to making the Baseball Hall of Fame is the opportunity to lobby for former teammates and peers to join the club.
Story | Conversation | July 28, 2007


text:
Monthly Archive for November, 2011
RPI included on new NBC Sports Group hockey schedule
The NBC Sports Group cable entity — currently called Versus, to become the NBC Sports Network on Jan. 2 — will televise college hockey for the first time, including two RPI home games. A 16-game schedule announced today includes RPI's games Jan. 6 against Dartmouth and Feb. 3 against Cornell. Both have been moved from […]
[Read More]
Show More


text:
Maximizing workflow with an EHR
FREE PREVIEW Log in or buy this issue to read the full article. AAFP members and paid subscribers get free access to all articles. Subscribe now.
Subscribe or
buy this issue. AAFP members and paid subscribers get free access to all articles.
Fam Pract Manag. 2007 Jun;14(6):13.
Dr. David E. Trachtenbarg's article "EHRs Fix Everything – and Nine Other Myths" [March 2007] describes ways to address the shortcomings of electronic health record (EHR) systems. Physicians should also understand that the implementation of an EHR creates an opportunity to optimize workflow.
With paper charts, physicians typically perform most, if not all, of the documentation of patient encounters. With EHRs, physicians can continue to be the primary author of all documentation if they choose, or they can implement a team approach to record keeping if their EHR is well constructed. A good EHR provides templates and flexibility to combine discrete data entry with free text.
EHRs are often marketed as being simple and streamlined with structured templates for a variety of medical conditions (e.g., hypertension and diabetes). Ironically, with the exception of some acute visits, the typical office visit of a family physician is neither simple nor streamlined. It often includes managing multiple chronic conditions, preventive care and acute problems, and it is rarely a one-problem visit.
When our group transitioned to an EHR, my greatest frustration was feeling constrained by these templates. Once I let go of this frustration, my EHR-induced anxiety waned. I began to focus on how my staff team and our EHR could optimize the care I provide my patients.
There are numerous permutations possible. I huddle with my staff before seeing patients each day to review the schedule and determine which encounters will have a coordinating EHR template. Our medical assistant collects the preliminary information at the beginning of each patient visit, which I review before entering the exam room, and then I clarify and confirm the information during my patient interview. The assistant accompanies me in the exam room and records the encounter.
This workflow process has been liberating. I spend my time focused on the patient, not distracted by documentation or the computer. My patients seem to appreciate the improved cycle time and don't mind having an assistant present. By the time I leave the room, most of the documentation is completed.
The success of this model is highly staff dependent. With team documentation and the tools nested in the EHR, I am able to more effectively provide comprehensive and quality patient care.
Send your comments to email@example.com. Submission of a letter will be construed as granting AAFP permission to publish the letter in any of its publications in any form. We cannot respond to all letters we receive. Those chosen for publication will be edited for length and style.
Copyright © 2007 by the American Academy of Family Physicians.
This content is owned by the AAFP. A person viewing it online may make one printout of the material and may use that printout only for his or her personal, non-commercial reference. This material may not otherwise be downloaded, copied, printed, stored, transmitted or reproduced in any medium, whether now known or later invented, except as authorized in writing by the AAFP. Contact
firstname.lastname@example.com for copyright questions and/or permission requests.
Want to use this article elsewhere? Get Permissions
Article Tools
CME Quiz
Information From Industry
FPM Resources
Stay Connected


text:
POPSUGAR Tech Slideshow The Week in Geek by Tech 11/22/08 0 Shares Like us on Facebook Previous Photo 1 of 11 View on One Page ADVERTISEMENT ( more slides) Next Share This Link Copy 1 The Week in Geek What a week, geeks! There was a ton of political gadget intrigue, Google debuts galore, not to mention a certain stormy cell phone on the scene . . . check out this week's greatest hits to make sure you didn't miss a thing! Previous Photo 1 of 11 View on One Page ADVERTISEMENT ( more slides) Next Read More SlideshowWeekly Recap Get inspired with our daily newsletter Enjoy gorgeous decor ideas, fast and easy recipes, and more in your inbox. Tech Home Food Vegetarian DIY Love & Sex Sign up with gallaugher 6 years Hi, Great site, but "The Week in Geek" is a copyright site, in use since 1997, and tied to my two domain names (weekingeek.com and theweekingeek.com) & book projects. Your work is great, but kindly do not call it "The Week in Geek". Thanks much! Prof. John Gallaugher Boston College Link Love Check out everything great about the new HTC One M9 — Newser A pilot plans to fly around the world using just solar power — Not Impossible Now This new app from Virgin Atlantic chooses your vacation for you — Co.Create 007-inspired hidden rooms that will blow your mind — TechEBlog Google plans to offer its own cellular network plan — Huff Post Tech How to delete your own Instagram comments — BuzzFeed Tech This artist may be the biggest Disney fan ever — POPSUGAR Love & Sex Latest Tech
'Orange Is The New Black' And 'Wet Hot American Summer' Get Netflix Premiere Dates HuffPost Tech 3.8K Shares


text:
Media & Resources for Journalists
Ministers Set to Tear Up UN Commitment On Climate Change
EU could be allowed to increase its emissions of global warming gases.
WWF Urges Urgent WTO Reform
The World Trade Organization needs substantial reform in the field of trade and environment ...
Ministers Set To Tear Up U.N. Commitment On Climate Change
Environment Ministers could allow the EU to increase its emissions of global warming gases..
WWF condemns Norwegian wolf culling
The conservation organization WWF condemns the decision of the Norwegian authorities to cull a pair ...
Global Warming: Early Action Bill
Washington - WWF today called for major changes to The Credit for Voluntary Early Action Act..
EU Countries Blamed For Habitats Scandal
Conservation organisation WWF today blamed European Union member states for failing to set up ...
Southernmost point of Africa declared a Gift to the Earth
The Agulhas National Park was officially launched at the Cape Agulhas Lighthouse on Monday.
WWF Stamp Collection helps conservation
The sale of stamps has become an important source of income for funding WWF's conservation ...
New study finds controls inadequate to protect Galapagos sea cucumbers
Sea cucumbers in the Galapagos Islands in Ecuador continue to be illegally fished and are also ...
WWF launches new campaign for Europe's large carnivores
The future of Europe's flagship carnivores such as lynxes, wolves and brown bears has reached a ...
Press Contact
+41 22 364 9562 or firstname.lastname@example.com
Emergency Contact (1800 CET onwards and weekends):
+41 79 559 6360
Sign-up to receive stories and press releases


text:
www.InvestorIdeas.com is a meeting place for investors and public companies in leading sectors. Find investing ideas, news and research for biotech stocks, tech and mobile stocks, mining stocks, oil and gas, water stocks, renewable energy, beverage stocks, defense stocks, nanotech and more on the TSX, OTC, NASDAQ and global stock exchanges. We have recently expanded our coverage to include Latin American markets.
Investor Ideas About InvestorIdeas.com:
InvestorIdeas.com is a leader in investor stock research by sector. Sectors we cover include; cleantech and renewable energy stocks, biotech stocks, mining and gold stocks, energy stocks, water, tech, defense stocks, nanotech, agriculture and gaming.
Follow Investorideas.com on ...More
Investor Ideas Newswire Investorideas Newswire - stock news, business news and investing ideas in leading sectors including water, renewable energy, biotech, tech, defense, energy and mining
Currently, there are no book details for Investorideas.com.
more »
A Revolution In Payment Systems; Biometric Experts Talk About Apple Pay And Smart Wallets $AAPL, $NXTD http://seekingalpha.com/p/20lyn
Oct 22, 2014
Latest comments on Investorideas.com's Instablog posts
more »
- A Revolution In Payment Systems; Biometric Experts Talk About Apple Pay And Smart Wallets
- Experts And Analyst Comment On Recent Security Breaches For National Cybersecurity Month
- Technology Alert; Biometric Experts Comment On Launch Of Apple Pay Smart Wallet Payment Service
- CyberSecurity Stocks Directory Update; Cyber Security Market In The US Government Sector To Grow At A CAGR Of 7.41 Percent 2013-2018
- Biotech News Alert: GenSpera's (OTCQB:GNSZ) CEO Shares Insight On Unique Properties And Targeting Of Lead Drug G-202, Based On The Thapsigargin Toxin


text:
Tag: cron
Terminal Tips: bash cron script to keep an app running
Do you have some apps that you want to keep running all the time? If so, and if you're not afraid of the Terminal or the command line, I have a script for you. When I come home at the end of the day, Dropbox has stopped running on my iMac. I'm running the latest version, and it works fine on my Mac...
Manually schedule Software Update 'the OS X way' with launchd
In response to a Macworld article, TidBits' Chris Pepper elaborated on ways to run Software Update, Apple's means of delivering updates and patches, on your own schedule. Beginning with the fact that Software Update schedules its next update based on the time it's currently being run, setting the ti...
Friday Favorite: MainMenu 2 keeps your Mac running smoothly
I'm a sucker for Mac maintenance utilities. That's not to say that I run them on a regular basis like I should do, but whenever I find a new one I like to give it a try and see how it's going to work for me. Dare To Be Creative Ltd. recently released version 2.0 of MainMenu, a collection of Mac util...
Software Updates
more updates- Apple Remote Desktop updated with Yosemite support
- OS X Yosemite 10.10.2, iOS 8.1.3 updates now available
- Sports Illustrated 120 SPORTS channel comes to Apple TV
- Logic Pro X update brings AirDrop support, new effects, tools, and more
- Parallels Access 2.5 released, adds file manager, computer-to-computer remote access
- The Google Translate iOS app is about to get a lot smarter


text:
A card heavy on TUF contestants could mean pink slips aplenty for UFC 180. That's one crazy undercard, but you can't deny the fireworks that the main card's got going on. Had Lauzon vs. Sanchez remained intact, this probably would've been a lock for best card of the year. It still could be, action-wise. With all that in mind, this will be the first pay-per-view I've watched since July's Weidman vs. Machida. Also, I'm going to start including fighters' TUF records if they're coming straight off t read news >>
If you're a Chad Mendes fan, there is "Money" to be made at UFC 179. That's assuming he can do what he failed to accomplish the first time around, and that's polish off Jose Aldo to claim the featherweight crown. He'll get the opportunity on Sat., Oct. 25, 2014 live on pay-per-view (PPV) from inside Maracanãzinho Gymnasium in Rio de Janeiro, Brazil (more on that here). Mendes originally opened at +150, but has since slipped to +250 underdog, according to Best Fight Odds. Aldo -- the -325 favorit read news >>
Thanks to his title loss at WSOF 10 this past Saturday, Georgi Karakhanyan suffered a slip in the rankings and Jeremy Stephens climbed a spot ahead of his biggest featherweight fight yet. Filed under: AXS TV Fights, Bellator, MMA Rankings, News, UFC, WSOF read news >>
Black swan health problems can be the worst thing in an athlete's life, arresting or ruining careers and lives. Giving MMA athletes better healthcare to mitigate the effects of black swan problems doesn't need a union. It needs selective targeting of promoters, managers, commission members and coaches with education and information about better options for athletes. Unseen, in the background, Fate was quietly slipping the lead into the boxing-glove. - P.G. Wodehouse, Very Good Jeeves!, pg. 421 A read news >>
Demetrious Johnson showed what it takes to hold onto a championship after taking some serious early damage from John Dodson to retain his UFC flyweight title at UFC on Fox 6. read news >>
Ratings for the seventh episode of "The Ultimate Fighter 16: Team Carwin vs. Team Nelson" slipped from the previous week's number with 676,000 total viewers tuning into the broadcast. The figure marks a 17 percent decrease in viewers from the 811,000 that tuned into episode No. 6. MMAjunkie.com today confirmed the ratings information with industry sources. read news >>
From the moment a fighter slips on a pair of UFC gloves, there's a saying hammered into his head: Never leave it in the hands of the judges. Fighters are reminded before they perform at an event. It's painted on the walls of the UFC training center and constantly repeated by UFC president Dana White. Judges would be out of work if the promotion had its way. read news >>
Brazil Fight has quickly become the premier MMA event in the state of Minas Gerais. Saturday night, at the Chevrolet Hall in Belo Horizonte, the in-state fighters triumphed 4-1 over their Sao Paulo foes in a five-fight interstate series. read news >>