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Well, my teenage sister Ashley, has run away for the third time. The first two times it was shortly before Christmas, and my parents knew she was at her boyfriend's house so they just drove there and brought her back the same day. This time around there isn't anything they can do because Friday was her 18th birthday. She claims that she's depressed and "can't stand" living with my parents because they are "too strict" and are "insensitive". I personally think that's a crock of sh*t. If anything my parents have been far too lenient with her. She's the baby, she has always gotten her way, she can do no wrong. She has a sour attitude towards my parents, myself, and our other two sisters. None of us can hold a conversation with her without her saying something nasty, negative, or rude. Basically she just goes all "emo" every time the littlest thing doesn't go her way, and I think she's just using this so-called "depression" to manipulate my parents. My parents have let her boyfriend make himself at home at their house, at the discomfort and expense of our other sisters who dislike him and feel uncomfortable with him around everyday. They asked my parents to limit his visits to 2 times a week, and they did nothing. They brought him to my apartment for a visit WITHOUT even asking me if that was ok, if it weren't for my older sister telling me about his coming I wouldn't have known. I asked my mother not to bring him, as I wanted family only time (my OWN boyfriend was not even invited for this particular visit) and she said it would be rude to un-invite him, he was like family, and brought him anyway. My parents took him out to dinner with them, on family trips and outings, and basically put out three of their other children to spoil their precious baby and her boyfriend. My sisters and I don't like him because he has made some rude remarks about my parents "harsh rules", my two other sisters, and tells numerous lies and untrue stories. He posted on facebook that my Dad's rules were "f***ing stupid" and had said all sorts of expletives about my mother as well, and really for no reason considering how generous my parents were to him. Ashley's grades have plummeted since dating him, her behavior has worsened, and now she has put my family through endless drama threatening to kill herself and running away multiple times. I'm at the end of my rope because as angry as I am with Ashley, I'm almost as equally angry with my parents. My sisters and I told them from the beginning he rubbed us the wrong way, and was making inappropriate comments. We told them that they should be keeping an eye on them and setting boundaries. They should talk to her about having a boyfriend for the first time, having sex, ect. They just hand waived it and said "oh she's a smart kid! She'll figure it out!" YES. She's so smart that she dropped out of high school with only 3 months to go, and dumped her family for an unwashed miscreant that has no job, no car, no education!!! Now I have to deal with my mother whining and wailing about the whole situation. We told our parents the first two times she ran away to set boundaries, take away her computer, cell phone, ect. And they said "well she promised not to run away again!!" so they didn't even really do anything proactive to help the situation. My mom is going through menopause, and she's hysterical about it all. As much as I want to be supportive, I offered my support before, and it was thrown back in my face. I know Ashley will come back eventually when her and the loser boyfriend run out of money. I want to tell my parents if they let her back in that house they will never see me there again. I'm done with Ashley, unless or until she cleans up her act. It's not fair to my other sisters who live in the same home (one of whom has a mild autism) to have to get the shaft in favor of someone who could clearly care less about them. At this point I don't really know what to say to my sister if she comes back, or even my mother, who I'm quite angry at, but at the same time I do feel for her. I'm trying to be supportive/understanding, but in all honesty it's really hard to not say "I TOLD YOU SO!!!!" If anyone else has been through a similar situation, or is good with advice in general...I sure could use some
Well, as you say, she's 18, so there's basically nothing they can do (unless she has a credit card that they pay, in which case they can just cancel it and cut her off. I would hope if they're paying for her cell phone they quit doing that, too.) I'd ignore her if she contacts you, and let her ruin her own life if that's what she wants to do. You CERTAINLY have no obligation to her boyfriend and forcing you to include him in a family visit was way out of line. As far as telling your parents you won't come to their house if/when they take her back in....well, that's up to you, and I would look out for guilt trip attempts, but you should do what is best for your own mental health. You could always say that your parents (minus baby sister and any romantic barnacles she happens to have) are welcome to visit you, but you are not going to their house until that situation changes, and stick to that. Though she might not come back this time, you never know, so it may be a moot issue. In any case, do what causes you the least possible amount of mental/emotional stress. Seen too many relatives work themselves into ulcers over dealing with poison family members.
Boundaries and rules won't help so unfortunately she will have to hit rock bottom before she comes around, have you asked your parents if they are prepared to raise a baby if your sister shows up broke, pregnant and dumped?
At this point, you need to do what's best for you. You can't force your parents to set boundaries or your sister to behave, but you can set them for yourself. You don't have to see your sister, your boyfriend, or your parents when they are with either the brat or the douche. Just like your sister is now an adult, your parents are adults. They made some choices that were perhaps not the wisest and they have to live with the consequences. Not to this extreme, but my little brother went through something like that as well, and my parents did eventually reach the point where they cut him off. His behavior lasted a hot minute after the money/help was cut off. My best friend's youngest brother went off the rails, and his parents are still bailing him out. He's 27, has Chrone's disease, a heroin addiction, a rap sheet a mile long (including driving a car into a family house and armed robbery), and at least two kids that we know about and one of them is special needs. I'm not saying that your sister will ever be that extreme, but it took a huge emotional toll on my friend's emotional well-being and health. She eventually had to say, I will not see my brother. I will not communicate with him. I will not have anything to do with him. She's been a lot better since she's done that. Her parents were upset by this, but my friend has her own family and their health/safety to consider. There's not a lot you can do for your parents, but you can and should take care of yourself.
Focus on you, I agree. Set rules for your house. If you don't want to see the boyfriend say he is not welcome in your house. I would advice, for a possible future relationship simply stating that to you sister, and also tell her hat as long as she follows you rules she is welcome, and that you love her. Just don't give her an inch, and remember to say that you don't like her choices, but you like her. If you are interested in a relationship 20 years down the line. I think your parents should do the same (and give her the safe sex talk), but you can't control them. If you get frustrated with them, make rules for them too - as in I love you, you are my parents, but I don agree with how you handle Ashley. Please don't speak of her troubles when you are in m house/talking with me.
Oh my goodness, this thread reminds me of a similar situation that my mum's closest friend experienced with her teenage daughter 27 years ago. Fast forward to 2013 and the daughter has no job, is an ex- heroin addict, unmarried with three children (and one with learning disabilities) to three different men, and still expects financial handouts from her parents. It's so sad because the phrase, 'a person bent on revenge digs two graves,' springs to mind.
I'm sorry, Smiley. My sister did some very similar things -- I remember how awful it was. My family had a very wise psychiatrist who told my mother, "You have to let her hit rock bottom." That is an unbelievably hard thing to do -- it was hard enough for me as her sister, I can't begin to imagine how hard it was for my parents. But they did it. Rock bottom was a long, long way down. But once she got there, she turned her life around. Thank God, she is doing SO much better now. All that to say, you have the right idea. You can't make your parents do anything, but keep doing what you can to encourage them to set boundaries and let her go. And encourage them to go to a good counselor too. I hope and pray that things will get better.
Do you live at home? How old are your sisters? A lot of sick people there. You are not going to be able to fix it. They need help. Save yourself. You are the only person you can help. Good luck
Pretty common dysfunctional family triangle: you have your sister whom you acknowledge is effed-up, your mother who is enabling her, and you are your mother's release valve/doormat. You believe that Mom is "normal" except for this situation with your sister, right? That once you can convince your mother to stop enabling your sister, everything in the family will be hunky-dory? Forget it. Your mother is an enabler, which is just as effed-up as the acknowledged effed-up person. Arguably the enabler is more dangerous because they'll convince you that they're "about to" set boundaries, but they never will. See the classic cartoon series of Charlie Brown, Lucy and the football. If you don't want to be the figurative Charlie Brown in this scenario, you'll set your own boundaries and protect yourself from this mess.
I can totally understand your frustration. My sister when she was about 14-15 was behaving like she was being incredibly hard done by at home which just wasn't true. My mum was a single mother and on a single mothers pension as well as working as a house cleaner to earn extra money for us. My sister had no appreciation of what my mum was doing. She was also quite violent which was very frightening. I overhead some girls at school talking about my "poor" sister and I let rip into them about how they had no clue and how difficult my sister was making it for the rest of our family. Why they even spoke that way in front of me when they probably knew I was her sister was really stupid. I agree with the thoughts here that she needs to find her own way and sort out her own life. She is 18 so there is not much you can do about it. She obviously has a sense of self-entitlement that you cannot do much about and she has taken advantage of your parent's attitude. It is good that you can blow off some steam here but at the end of the day you have to also learn how to deal with the situation and accept that you can't change her with her current attitude and state of mind.
Thanks for the advice everyone Exactly. Although I'm pretty straight forward with my mom. She wants advice, so I'm not going to sugar coat it, and neither do my other sisters when she's crying to them about the situation. Not by a long shot! In all honesty I think my mom is a huge hypocrite for enabling my sister, and then constantly bitching about my Grandmother (her mother in law) enabling my Aunt, who has her own issues. That's a whole separate drama, but my mom was constantly bitching about how she had to "deal with it" because my Grandmother lives with my family. Long story short my sisters and I kept giving her advice that she wouldn't take, so I finally blew up at her and told her I didn't want to hear another word about the situation if she refused to be proactive. She scaled back her bitching about that topic, but she still gripes from time to time. My mom has a ton of issues, and for the most part I can deal with it, but this is just out of control, especially because my sisters and I feel as if we're getting the shaft in favor of Ashley. It's always been like that, but now it's egregious. Perfect analogy. At this point my main concern is the welfare of my other sisters. One of them has broken into hives from the stress. I don't live with my mom so at least I can limit the phone calls and just tell her I'm busy if it gets to be too much. I don't even want to visit my parents for Easter, because I just don't want to get sucked in to the drama. I can't face my parents and listen to them "woe is me" when it truly does bother me that my parents blatantly ignored our wishes and put Ashley's needs before ours. It hurts, but I just have to accept that it's probably always going to be like that.
That's really good advice. My mom is seeing a therapist and my sister Ashley was seeing the same one as well. To be honest neither of them have made any progress. But I guess if you don't want to be helped there's not much a therapist can do. Taking her to the therapist was probably the one pro-active thing they did, but I'm not sure if it was a good idea for my sister to be seeing the same therapist as my mother. Their reasoning for being so "soft" on her was her threats to kill herself if she didn't get her way. It seemed to me she was manipulating them, but I can understand not dismissing a suicide threat. Everyone in our family was telling them to have her committed if they TRULY felt she was a danger to herself, but my mom decided against it, because "She said she didn't want to go!" Well, NO SH*T sherlock! What person that needs to be committed actually knows whats best for them? Really?! I hate to sound like a jerk, but if she wanted to kill herself she would have tried already. My older sister tried twice when she was a teenager and my parents treated her quite harshly in comparison.
So, don't listen to their "woe is me" monologues. Hang up the phone, leave the room, whatever. You've already discussed this to exhaustion. By providing an outlet for their "woe is me" garbage, you are acting as a release valve. They can pay for a therapist if that want that particular service, plus they might actually accomplish something in counseling.
Just repeating the above as it is right on. Can your sisters move out of your parents' house? Sounds like both of them are over 18 if Ashley is the baby. Even with her gone, it sounds like the house isn't a terribly good environment for them. You say that this guy is Ashley's first boyfriend? Eek, what a nightmare.
They're both working on moving out, but I don't see them as getting to that point anytime soon since my parents haven't bothered to equip them with any sort of life skills to transition into adulthood. One of them is 21, never had a job in her life, and is getting by with fairly average grades at the local community college. She's a good, respectful kid, but very sheltered, somewhat spoiled (not to the extent of Ashley) and to be honest kind of lazy. My older sister has Aspergers, and really has gotten the shaft out of all of us. My parents treated her quite harshly has a child/teenager, and while they brought her to psychiatrists and doctors, they didn't really do any of their own research on Asperger's nor did they offer much support when she was being bullied in school. She can't work because she recieves government assistance, but she's a full time student and is quite intellegent. It's definitely not a good enviornment for them, but hopefully they can get it together soon and move out on their own.


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It's happening whether anyone's ready or not. Businesses here in Oregon and across the U.S. are already experiencing the effects of the largest generational shift in recent history, and these changing tides will impact every level of the workplace — from a company's executive leadership to its cultural core.
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Created in this Craftsy Course
Bag-Making Basics: Reversible Tote & Zipper Pouch
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Take this free mini-class and learn how to make two cute bags with Kristin Link of Sew,Mama,Sew!
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Thank you so much Kristin for your excellent step-by-step teaching! I'm so proud of actually putting a zipper into this lined pouch! Couldn't have done it without your help! So grateful to Craftsy for giving these free classes!


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Sea-Saw Rocker
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Move the playground from land to water with this exceptionally durable and enormously fun pool inflatable! Have a rocking and rolling good time with Swimlines inflatable Sea Saw Rocker! Your kids will love this big new inflatable pool float. Made of heavy gauge vinyl, the rocker has built-in handles that double the fun. Great for the kids! This huge float is 57 in W x 90 in L x 36 in H. Seat is approximately 17 inches.Features-Rockin n rollin outrageous swimming pool fun-Holds two children-Extra strong PVC vinyl construction-Heavy Duty handles-For Ages 8 & Up-57 in W x 90 in L x 36 in H
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NORTH CHARLESTON, S.C. (AP) -- Boeing workers in North Charleston are assembling the first new 787 to be made in South Carolina.
The Post and Courier of Charleston reported (http://bit.ly/pNWt2T ) that the completed aft section of the plane body was wheeled into the assembly plant this week.
In the past, those sections were made in Charleston and flown to Washington state for assembly of a finished aircraft. Spokeswoman Candy Eslinger says the aft section was the last major section needed for final assembly.
The company this week also opened its welcome center. That building houses a security checkpoint as well as meeting rooms.
Earlier this year, Boeing dedicated its $750 million plant, the largest industrial investment in state history.
Information from: The Post and Courier, http://www.postandcourier.com


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PREVIEW: The Psychedelic Furs @ House of Blues – 11/2/12
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WHERE: House of Blues
WHEN: Friday, November 2nd – Show at 8:00
The Psychedelic Furs have tested the sands of time and have reached a truly classic phase in life. Supporting material for a new record, the Furs will be stopping by The House of Blues on Friday to remind us of their genius. They, for all intents and purposes, were New Wave even before the term ever was uttered from anyone's mouth, so come on down to House of Blues to catch the tide.
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US 20050239085 A1 Abstract Methods of the invention comprise methods for nucleic acid sequence determination. Generally, the invention relates to sequencing a target nucleic acid by exposing the target nucleic acid to a primer and a polymerase. Such methods may involve determining the sequence of a target nucleic acid by using a thermophilic polymerase, such as a variant of said 9° N DNA polymerase.
Claims(40) 1. A method for nucleic acid sequence determination, the method comprising the steps of:
(a) exposing a target nucleic acid to a primer that is complementary to at least a portion of the target, a thermophilic polymerase, and at least one nucleotide for extension of said primer; (b) conducting a primer extension at a temperature of about 20-70° C.; (c) detecting incorporation of said nucleotide in said primer; and, (d) repeating steps (a), (b) and (c), thereby to determine a sequence of said target. 2. The method of
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Description The present invention relates to methods for nucleic acid sequence determination. More specifically, the present invention relates to sequencing a target nucleic acid by exposing the target nucleic acid to a primer and a polymerase, such as a thermophilic polymerase. One of the most significant milestones in scientific history was the sequencing of the human genome. While the completion of the first human genome sequence is an important scientific milestone, many challenges remain in the areas of genetics and medicine. It is apparent that a true understanding of genetic function lies in the small variations in sequence that occur both within and between individuals. For example, relatively small genomic changes, such as single nucleotide polymorphisms, have been found to lead to profound changes in phenotype. Subtle and infrequent nucleotide changes also have been associated with cancer and other genetic diseases. Conventional nucleotide sequencing is accomplished through bulk techniques. For example, the two most common techniques for sequencing are the Maxam and Gilbert selective chemical degradation technique and the Sanger dideoxy sequencing technique. Bulk sequencing techniques are not useful for the identification of subtle or rare nucleotide changes due to the many cloning, amplification and electrophoresis steps that complicate the process of gaining useful information regarding individual nucleotides. As such, research has evolved toward methods for rapid sequencing, such as single molecule sequencing technologies. The ability to sequence and gain information from single molecules obtained from an individual patient is the next milestone for genomic sequencing. However, effective diagnosis and management of important diseases through single molecule sequencing is impeded by lack of cost-effective tools and methods for screening individual molecules. A number of nucleic acid polymerases have been isolated and purified from mesophilic and thermophilic organisms and applied to bulk sequencing, which utilizes amplification by polymerase chain reaction. Due to the denaturation cycle of polymerase chain reaction, a greater number of thermophilic polymerases have been investigated for their thermostable properties at high temperatures, which generally are greater than 70° C. For example, DNA polymerases have been isolated from thermophilic bacteria that are capable of growth at very high temperatures including Bacillus steraothermophilus that have a half-life of 15 minutes at 87°. Thus, there exists a need in the art to develop nucleic acid polymerases and methods of using nucleic acid polymerases that are cost-effective and successful for single molecule nucleic acid sequencing and analysis. The invention provides for the use of thermophillic polymerases in single molecule sequencing reactions. It has been discovered that thermophillic polymerases, which traditionally have been used for amplification reactions at high temperature (due to their thermostability), are highly-effective at lower temperatures in single molecule sequencing reactions. In a preferred embodiment, polymerization takes place at a temperature of between about 20° C. to about 70° C. Preferred methods of the invention comprise conducting a single molecule sequencing reaction in the presence of a thermophilic polymerase. Single molecule sequencing according to the invention comprises template-dependent nucleic acid synthesis. In a preferred embodiment, nucleic acid primers are exposed to template molecules having a primer binding site. Polymerase then directs the extension of the primer in a template-dependent fashion in the presence of labeled nucleotides or nucleotide analogs. According to the invention, primers are support-bound in a manner that allows unique optical identification of signaling events from the labeled nucleotide or nucleotide analogs as they are incorporated into the growing primer strand. In preferred methods of the invention, the thermophilic polymerase used in sequencing reactions is a 9° N DNA polymerase or a variant of the 9° N DNA polymerase. For example, a preferred variant of the 9° N DNA polymerase is an Archaeon polymerase with enhanced ability to incorporate modified nucleotides, such as a 9° N A485L (exo-) DNA polymerase. Preferred polymerases have a reduced 3′ to 5′ proofreading activity (exo-). Methods according to the present invention comprise exposing a target nucleic acid molecule and a polymerase and at least one nucleotide or nucleotide analog to each other under non-elevated temperature or ambient temperature. With bulk sequencing of nucleic acids, thermophilic polymerases are required for their thermostable properties at elevated temperatures necessary for amplification when conducting a polymerase chain reaction. However, methods according to the invention include conducting a primer extension at lower or ambient temperatures, such as a temperature of about 20-70° C. In some embodiments, primer extension is conducted at a temperatures of about 20-50° C., about 30-40° C., or preferably about 37° C. Methods according to the invention also comprise exposing a target nucleic acid to a primer and thermophilic polymerase to incorporate a modified nucleotide for extension of the primer. A modified nucleotide includes any nucleotide analog, such as a dideoxynucleotide, a ribonucleotide, and an acyclonucleotide. A modified nucleotide also can be a non-chain terminating nucleotide such as, for example, a deoxynucleotide including dATP, dTTP, dUTP, dCTP, and dGTP. In general, however, a modified nucleotide includes any base or modified base that exhibits Watson-Crick base pairing. Examples of nucleotide analogs include any modified base or synthetic analog such as, for example, a 7-deaxa-adenine, a 7-deaxa-guanine, inosine, xanthine, AMP, GMP, guanosine. In some embodiments, a nucleotide analog comprises a removable linker. Also, a nucleotide analog can be modified to remove, cap, or modify the 3′ hydroxyl group. By so doing the 3′ hydroxyl group from the incorporated nucleotide in the primer, further extension is halted or impeded. In certain embodiments, the modified nucleotide is engineered so that the 3′ hydroxyl group can be removed and/or added by chemical methods. Preferred methods of the invention comprise optically detecting incorporation of a nucleotide or nucleotide analog in a template-dependent primer extension reaction. In preferred embodiments, nucleotides are labeled for detection, preferably with a fluorescent label. In one embodiment, methods of the invention comprise detecting coincident fluorescence emission of a first fluorescent label and a second fluorescent label. The labels are attached to the polymerase and to the nucleotide base to be added. Coincident fluorescence emission preferably occurs between about 400 nm and about 900 nm. There are many detectable labels appropriate for use with the methods of the invention. Any optically-detectable label is useful in methods of the invention. Especially preferred are fluorescent labels and dyes. For example, rhodamine, BODIPY, alexa, or any other conjugated dye is used in order to facilitate optical detection of individual nucleotides. In certain preferred embodiments, a detectable label is selected from cyanine 5 and cyanine 3. Methods according to the invention also comprise removing or neutralizing a label subsequent to detecting it. Generally, a plurality of target nucleic acids is attached to a substrate or an array. Each member of the plurality is attached to a surface, such as glass or fused silica, preferably by covalent attachment. One skilled in the art understands that target nucleic acids can be attached to any surface that allows for primer extension, and preferably, to any surface suitable for detecting incorporation of nucleotides or nucleotide analogs. As such, in some embodiments, each member of the plurality of target nucleic acids is covalently attached to a surface that has reduced background fluorescence with respect to glass, polished glass or fused silica. Examples of surfaces appropriate for the invention include polytetrafluoroethylene or a derivative of polytetrafluoroethylene, such as silanized polytetrafluoroethylene. In addition, in preferred embodiments of the invention target nucleic acids are spaced apart on a substrate such that each target is optically resolvable. In practice, for example, the target may be optically resolved by detecting a fluorescent label attached to the nucleotide. When conducting a primer extension reaction, after detecting the incorporation of a label, preferred methods according to the invention comprise the step of washing unincorporated reagents, such as nucleotides, nucleotide analogs, labels, dyes and/or buffer from the substrate. In certain embodiments, methods according to the invention provide-for neutralizing a label by photobleaching. This may be accomplished by focusing a laser with a short laser pulse, for example, for a short duration of time with increasing laser intensity. In other embodiments, a label may be photocleaved. For example, a light-sensitive label bound to a nucleotide may be photocleaved by focusing a particular wavelength of light on the label. Generally, it may be preferable to use lasers having differing wavelengths for exciting and photocleaving. Labels may be removed from a substrate using reagents, such as NaOH or other appropriate buffer reagent. A detailed description of embodiments of the invention is provided below. Other embodiments of the invention are apparent upon review of the detailed description that follows. The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee. Single molecule sequencing requires highly-sensitive and cost-effective tools to provide rapid and accurate results. Single molecule sequencing has the potential to provide sequence-specific genomic information that is relevant to both normal and diseased function. Among the tools on which sequencing reactions are most dependent are polymerase enzymes. The present invention provides for use in low temperature single molecule sequencing thermophilic polymerases that were developed for bulk sequencing reactions that cycle through high amplification temperatures. One example of a thermophilic polymerase that traditionally is used for its thermostable properties at elevated temperatures is the 9 degrees north A485L (exo-) DNA polymerase. According to the invention, these thermophilic polymerases are useful in single molecule reactions conducted at lower temperatures that are typically thought to be optimal for the enzyme. The polymerase 9° N (exo-) /A485L is sold commercially by New England BioLabs (Beverly, Mass.) as Therminator™ and by Perkin-Elmer (Boston, Mass.) in AcycloPrime SNP kits as AcycloPol™. Generally, the variant of the 9° N DNA polymerase is isolated and purified from an E. coli strain that carries the 9° N A485L (exo-) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from Thermococcus species 9° N-7. In addition, the 9° N DNA polymerase and/or variant thereof can be purified free of contaminating endonucleases and exonucleases. Generally, amplification and cloning steps that are involved in polymerase chain reaction require providing thousands of copies of nucleic acids under denaturation conditions that expose the polymerase to high temperatures, such as temperatures greater than about 70° C. To meet the need for thermostability at elevated temperatures required in traditional polymerase chain reaction techniques, technicians and researchers have identified thermophilic polymerases that are thermostable and, therefore, retain their ability to incorporate nucleotides in a primer in elevated temperature conditions. However, methods according to the present invention utilize these thermophilic polymerases in primer extension reactions at non-elevated temperatures for sequencing single molecules. Accordingly, methods of the invention include conducting a primer extension reaction at a temperature of about 20-70° C. In some embodiments, primer extension using thermophilic polymerases, such as the 9 degrees north A485L (exo-) DNA polymerase, is conducted at a temperatures of about 20-50° C., at about 30-40° C., or preferably at about 37° C. Without the wasteful and expensive cloning and amplification steps required in current DNA sequencing technologies, methods according to the invention provide for simpler and less error-prone sequencing with greater applications in disease detection and diagnosis for individual analysis. Such methods are particularly useful in connection with a variety of biological samples, such as blood, urine, cerebrospinal fluid, seminal fluid, saliva, breast nipple aspirate, sputum, stool and biopsy tissue. Especially preferred are samples of luminal fluid because such samples are generally free of intact, healthy cells. However, any tissue or body fluid specimen may be used according to methods of the invention. Nevertheless, the target nucleic acid can come from a variety of sources. For example, nucleic acids can be naturally occurring DNA or RNA isolated from any source, recombinant molecules, cDNA, or synthetic analogs, as known in the art. For example, the target nucleic acid may be genomic DNA, genes, gene fragments, exons, introns, regulatory elements (such as promoters, enhancers, initiation and termination regions, expression regulatory factors, expression controls, and other control regions), DNA comprising one or more single-nucleotide polymorphisms (SNPs), allelic variants, and other mutations. Also included is the full genome of one or more cells, for example cells from different stages of diseases such as cancer. The target nucleic acid may also be mRNA, tRNA, rRNA, ribozymes, splice variants, antisense RNA, and RNAi. Also contemplated according to the invention are RNA with a recognition site for binding a polymerase, transcripts of a single cell, organelle or microorganism, and all or portions of RNA complements of one or more cells, for example, cells from different stages of development or differentiation, and cells from different species. Nucleic acids can be obtained from any cell of a person, animal, plant, bacteria, or virus, including pathogenic microbes or other cellular organisms. Individual nucleic acids can be isolated for analysis. Methods according to the invention provide for the determination of the sequence of a single molecule, such as a target nucleic acid. Generally, target nucleic acids can have a length of about 5 bases, about 10 bases, about 20 bases, about 30 bases, about 40 bases, about 50 bases, about 60 bases, about 70 bases, about 80 bases, about 90 bases, about 100 bases, about 200 bases, about 500 bases, about 1 kb, about 3 kb, about 10 kb, or about 20 kb and so on. Methods according to the invention include exposing a target nucleic acid to a primer. In general, the primer is complementary to at least a portion of the target nucleic acid. The target nucleic acid also is exposed to a thermophilic polymerase (as discussed herein) and at least one nucleotide or nucleotide analog allowing for extension of the primer. A nucleotide or nucleotide analog includes any base or base-type including adenine, cytosine, guanine, uracil, or thymine bases. In addition, additional nucleotide analogs include xanthine or hypoxanthine, 5-bromouracil, 2-aminopurine, deoxyinosine, or methylated cytosine, such as 5-methylcytosine, N4-methoxydeoxycytosine, and the like. Also included are bases of polynucleotide mimetics, such as methylated nucleic acids, e.g., 2′-O-methRNA, peptide nucleic acids, modified peptide nucleic acids, and any other structural moiety that can act substantially like a nucleotide or base, for example, by exhibiting base-complementarity with one or more bases that occur in DNA or RNA and/or being capable of base-complementary incorporation. Methods of the invention also include detecting incorporation of the nucleotide or nucleotide analog in the primer and, repeating the exposing, conducting and/or detecting steps to determine a sequence of the target nucleic acid. By using the right tools in single molecule sequencing, a researcher can compile the sequence of a complement of the target nucleic acid based upon sequential incorporation of the nucleotides into the primer. Similarly, the researcher can compile the sequence of the target nucleic acid based upon the complement sequence. Also, a nucleotide analog can be modified to remove, cap or modify the 3′ hydroxyl group. As such, in certain embodiments, methods of the invention can include, for example, the step of removing the 3′ hydroxyl group from the incorporate nucleotide or nucleotide analog. By removing the 3′ hydroxyl group from the incorporated nucleotide in the primer, further extension is halted or impeded. In certain embodiments, the modified nucleotide can be engineered so that the 3′ hydroxyl group can be removed and/or added by chemical methods. In addition, a nucleotide analog can be modified to include a moiety that is sufficiently large to prevent or sterically hinder further chain elongation by interfering with the polymerase, thereby halting incorporation of additional nucleotides or nucleotide analogs. Subsequent removal of the moiety, or at least the steric-hindering portion of the moiety, can concomitantly reverse chain termination and allow chain elongation to proceed. In some embodiments, the moiety also can be a label. As such, in those embodiments, chemically cleaving or photocleaving the blocking moiety may also chemically-bleach or photo-bleach the label, respectively. The nucleic acids suitable for analysis with the invention can be DNA or RNA, as discussed herein. The methods according to the invention can provide de novo sequencing, sequence analysis, DNA fingerprinting, polymorphism identification, for example single nucleotide polymorphisms (SNP) detection, as well as applications for genetic cancer research. Applied to RNA sequences, methods according to the invention also can identify alternate splice sites, enumerate copy number, measure gene expression, identify unknown RNA molecules present in cells at low copy number, annotate genomes by determining which sequences are actually transcribed, determine phylogenic relationships, elucidate differentiation of cells, and facilitate tissue engineering. The methods according to the invention also can be used to analyze activities of other biomacromolecules such as RNA translation and protein assembly. Certain aspects of the invention lead to more sensitive detection of incorporated signals and faster sequencing. Methods of the invention also include conducting primer extension reactions with target nucleic acids that are attached to a substrate, surface, support or an array. Each member of the plurality of target nucleic acids can be covalently attached to a surface including glass or fused silica. For example, each member of the plurality of target nucleic acids can be covalently attached to a surface that has reduced background fluorescence with respect to glass, polished glass, fused silica or plastic. Examples of surfaces appropriate for the invention include, for example, polytetrafluoroethylene or a derivative of polytetrafluoroethylene, such as silanized polytetrafluoroethylene. Locations on a substrate, surface, support or array include a target nucleic acid that is linked thereto. In some embodiments, the locations include a primer, a target polynucleotide-primer complex, and/or a polymerase bound thereto. These moieties can be bound or immobilized on the surface of the substrate or array by covalent bonding, non-covalent bonding, ionic bonding, hydrogen bonding, van der Waals forces, hydrophobic bonding, or a combination thereof. The immobilizing may utilize one or more binding-pairs, including, but not limited to, an antigen-antibody binding pair, a streptavidin-biotin binding pair, photoactivated coupling molecules, and a pair of complementary nucleic acids. Furthermore, the substrate or support may include a semi-solid support (e.g., a gel or other matrix), and/or a porous support (e.g., a nylon membrane or other membrane). The surface of the substrate or support may be planar, curved, pointed, or any suitable two-dimensional or three-dimensional geometry. A single molecule substrate or array describes a support or an array in which all or a subset of molecules of the array can be individually resolved and/or detected. According to invention, methods include the step of detecting incorporation of a nucleotide or nucleotide analog in a primer. Generally, the detection system includes any device that can detect and/or record light emitted from a nucleotide, from a target nucleic acid and/or a primer, and/or a polymerase. Accordingly, a detection system has single-molecule resolution or the ability to resolve one molecule from another. For example, in certain embodiments, the detection limit is in the order of a micron. Therefore, two molecules can be a few microns apart and be resolved, that is individually detected and/or detectably distinguished from each other. Certain embodiments of the invention are described in the following examples, which are not meant to be limiting. Experiments were conducted to determine whether thermophilic polymerases are capable of incorporating nucleotides in primer extension reactions for single molecule sequencing. Various thermophilic polymerases were screened, including the 9 degrees north A485L (exo-) DNA polymerase, and exposed to fluorescently labeled nucleotides. Incorporation of Nucleotides using Polymerases in Single Molecule Sequencing A target nucleic acid is obtained from a patient using a variety of known procedures for extracting the nucleic acid. Although unnecessary for single molecule sequencing, the extracted nucleic acid can be optionally amplified to a concentration convenient for genotyping or sequence work. Nucleic acid amplification methods are known in the art, such as polymerase chain reaction. Other amplification methods known in the art that can be used include ligase chain reaction, for example. The single stranded plasmid can be primed by 5′-biotinylated primers, and double stranded plasmid can then be synthesized. The double stranded plasmid can then be linearized, and the biotinylated strand purified. Analyzing a target nucleic acid by synthesizing its complementary strand may involve hybridizing a primer to the target nucleic acid. The primer can be selected to be sufficiently long to prime the synthesis of extension products in the presence of a thermophilic polymerase, such as a variant of 9° N DNA polymerase (9 degrees north A485L (exo-) DNA polymerase). Primer length can be selected to facilitate hybridization to a sufficiently complementary region of the template polynucleotide downstream of the region to be analyzed. The exact lengths of the primers depend on many factors, including temperature, source of primer. If part of the region downstream of the sequence to be analyzed is known, a specific primer can be constructed and hybridized to this region of the target nucleic acid. Alternatively, if sequences of the downstream region on the target nucleic acid are not known, universal or random primers may be used in random primer combinations. As another approach, a linker or adaptor can be joined to the ends of a target nucleic acid polynucleotide by a ligase and primers can be designed to bind to these adaptors. That is, a linker or adaptor can be ligated to at least one target nucleic acid of unknown sequence to allow for primer hybridization. Alternatively, known sequences may be biotinylated and ligated to the targets. In yet another approach, nucleic acid may be digested with a restriction endonuclease, and primers designed to hybridize with the known restriction sites that define the ends of the fragments produced. Primers can be synthetically made using conventional nucleic acid synthesis techniques. For example, primers can be synthesized on an automated DNA synthesizer, e.g. an Applied Biosystems, Inc. (Foster City, Calif.) model 392 or 394 DNA/RNA Synthesizer, using standard chemistries, such as phosphoramidite chemistry, and the like. Alternative chemistries, e.g., resulting in non-natural backbone groups, such as phosphorothioate, phosphoramidate, and the like, may also be employed provided that, for example, the resulting oligonucleotides are compatible with the polymerizing agent. The primers can also be ordered commercially from a variety of companies which specialize in custom nucleic acids such as Operon Inc (Alameda, Calif.). In some instances, the primer can include a label. When hybridized to a linked nucleic acid molecule, the label facilitates locating the bound molecule through imaging. The primer can be labeled with a fluorescent labeling moiety (e.g., Cy3 or Cy5), or any other means used to label nucleotides. The detectable label used to label the primer can be different from the label used on the nucleotides or nucleotide analogs used on the nucleotides in the subsequent extension reactions. Suitable fluorescent labels include, but are not limited to, 4-acetamido-4′-isothiocyanatostilbene-2,2′disulfonic acid; acridine and derivatives: acridine, acridine isothiocyanate; 5-(2′-aminoethyl)aminonaphthalene-1-sulfonic acid (EDANS); 4-amino-N-[3-vinylsulfonyl)phenyl]naphthalimide-3,5 disulfonate; N-(4-anilino-1-naphthyl)maleimide; anthranilamide; BODIPY; Brilliant Yellow; coumarin and derivatives; coumarin, 7-amino-4-methylcoumarin (AMC, Coumarin 120), 7-amino-4-trifluoromethylcouluarin (Coumaran 151); cyanine dyes; cyanosine; 4′,6-diaminidino-2-phenylindole (DAPI); 5′5″-dibromopyrogallol-sulfonaphthalein (Bromopyrogallol Red); 7-diethylamino-3-(4′-isothiocyanatophenyl)-4-methylcoumarin; diethylenetriamine pentaacetate; 4,4′-diisothiocyanatodihydro-stilbene-2,2′-disulfonic acid; 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid; 5-[dimethylamino]naphthalene-1-sulfonyl chloride (DNS, dansylchloride); 4-dimethylaminophenylazophenyl-4′-isothiocyanate (DABITC); eosin and derivatives; eosin, eosin isothiocyanate, erythrosin and derivatives; erythrosin B, erythrosin, isothiocyanate; ethidium; fluorescein and derivatives; 5-carboxyfluorescein (FAM), 5-(4,6-dichlorotriazin-2-yl)aminofluorescein (DTAF), 2′,7′-dimethoxy-4′5′-dichloro-6-carboxyfluorescein (JOE), fluorescein, fluorescein isothiocyanate, QFITC, (XRITC); fluorescamine; IR144; IR1446; Malachite Green isothiocyanate; 4-methylumbelliferoneortho cresolphthalein; nitrotyrosine; pararosaniline; Phenol Red; B-phycoerythrin; o-phthaldialdehyde; pyrene and derivatives: pyrene, pyrene butyrate, succinimidyl 1-pyrene; butyrate quantum dots; Reactive Red 4 (Cibacron™ Brilliant Red 3B-A) rhodamine and derivatives: 6-carboxy-X-rhodamine (ROX), 6-carboxyrhodamine (R6G), lissamine rhodamine B sulfonyl chloride rhodarnine (Rhod), rhodamine B, rhodamine 123, rhodamine X isothiocyanate, sulforhodamine B, sulforhodamine 101, sulfonyl chloride derivative of sulforhodamine 101 (Texas Red); N,N,N′,N′tetramethyl-6-carboxyrhodamine (TAMRA); tetramethyl rhodamine; tetramethyl rhodamine isothiocyanate (TRITC); riboflavin; rosolic acid; terbium chelate derivatives; Cy3; Cy5; Cy5.5; Cy7; IRD 700; IRD 800; La Jolta Blue; phthalo cyanine; and naphthalo cyanine. If the target polynucleotide-primer complex is to be linked on a surface of a substrate or array, the primer can be hybridized before or after such linking. Primer annealing can be performed under conditions which are stringent enough to require sufficient sequence specificity, yet permissive enough to allow formation of stable hybrids at an acceptable rate. The temperature and time required for primer annealing depend upon several factors including base composition, length, and concentration of the primer; the nature of the solvent used, e.g., the concentration of DMSO, formamide, or glycerol; as well as the concentrations of counter ions, such as magnesium. Typically, hybridization with synthetic polynucleotides is carried out at a temperature that is approximately 5° C. to approximately 10° C. below the melting temperature (Tm) of the target polynucleotide-primer complex in the annealing solvent. However, according to methods of the invention, hybridization may be performed at much lower temperatures, such as for example 30-50° C. or 30-40° C. The annealing reaction can be complete within a few seconds. After preparing the target nucleic acid and optionally linking it on a substrate, primer extension reactions can be performed to analyze the target polynucleotide sequence by synthesizing its complementary strand. The primer is extended by a thermophilic polymerase in the presence of a nucleotide or nucleotide analog bearing a detectable label at a temperature of about 10 to about 70° C., about 20 to about 60° C., about 30 to about 50° C., or preferably at about 37° C. In other embodiments, two, three or all four types of nucleotides are present, each bearing a detectably distinguishable label. In some embodiments of the invention, a combination of labeled and non-labeled nucleotides or nucleotide analogs are used in the primer extension reaction for analysis. Depending on the template, a DNA polymerase, an RNA polymerase, or a reverse transcriptase can be used in the primer extension reactions. Preferably, a thermophilic polymerase is used according to the invention. And more preferably, a 9° N DNA polymerase or variant thereof is used as the polymerizing agent. For example, in one embodiment, a variant of the 9° N DNA polymerase that is an Archaeon polymerase with enhanced ability to incorporate a modified nucleotide can be used in the primer extension reaction at a temperature of about 37° C. An Archaeon polymerase may be a 9 degrees north A485L (exo-) DNA polymerase, for example. Generally, the polymerase according to the invention has high incorporation accuracy and a processivity (number of nucleotides incorporated before the polymerase dissociates from the target nucleic acid) of at least about 20 nucleotides. Nucleotides can be selected to be compatible with the polymerase, for example, the 9 degrees north A485L (exo-) DNA polymerase. The incorporation of the labeled nucleotide or nucleotide analog can be detected on the primer. A number of systems are available to accomplish this. Methods for visualizing single molecules of labeled nucleotides with an intercalating dye include, e.g., fluorescence microscopy. In some embodiments, the fluorescent spectrum and lifetime of a single molecule excited-state can be measured. Standard detectors such as a photomultiplier tube or avalanche photodiode can be used. Full field imaging with a two-stage image intensified CCD camera can also used. Additionally, low noise cooled CCD can also be used to detect single fluorescent molecules. The detection system for the signal may depend upon the labeling moiety used, which can be defined by the chemistry available. For optical signals, a combination of an optical fiber or charged couple device (CCD) can be used in the detection step. In the embodiments where the substrate is itself transparent to the radiation used, it is possible to have an incident light beam pass through the substrate with the detector located opposite the substrate from the primer. For electromagnetic labels, various forms of spectroscopy systems can be used. Various physical orientations for the detection system are available and known in the art. A number of approaches can be used to detect incorporation of fluorescently-labeled nucleotides into a single molecule. Optical systems include near-field scanning microscopy, far-field confocal microscopy, wide-field epi-illumination, light scattering, dark field microscopy, photoconversion, single and/or multiphoton excitation, spectral wavelength discrimination, fluorophore identification, evanescent wave illumination, and total internal reflection fluorescence (TIRF) microscopy. In general, methods involve detection of laser-activated fluorescence using a microscope equipped with a camera, sometimes referred to as high-efficiency photon detection system. Suitable photon detection systems include, but are not limited to, photodiodes and intensified CCD cameras. For example, an intensified charge couple device (ICCD) camera can be used. The use of an ICCD camera to image individual fluorescent dye molecules in a fluid near a surface provides numerous advantages. For example, with an ICCD optical setup, it is possible to acquire a sequence of images (movies) of fluorophores. Determining Processivity of 9° N A485 (exo-) DNA Polymerase in the Presence of Labeled Nucleotides As a proof-of-principle to determine whether the 9° N A485 (exo-) DNA polymerase accurately incorporates labeled nucleotides into the primer, an extension experiment can be performed in a test tube rather than on a substrate. In this experiment, incorporation of dCTP-Cy3 and a polymerization terminator, ddCTP, can be detected using a 7G DNA template (a DNA strand having a G residue every 7 bases). The annealed primer is extended in the presence of non-labeled dATP, dGTP, dTTP, Cy3-labeled dCTP, and ddCTP. The ratio of Cy3-dCTP and ddCTP can be analyzed. The reaction products can be separated on a gel, fluorescence can be excited, and the signals detected, using an automatic sequencer, such as, ABI-377. The presence of fluorescence intensity from primer extension products of various lengths which were terminated by incorporation of ddCTP at the different G residues in the 7G oligomer template can be analyzed, for example, on a gel. Bands correlating to extension products suggest the incorporation of nucleotides, and the different bands suggest incorporation of nucleotides of differing lengths. A screening process was established and the 9 degrees north A485L (exo-) DNA polymerase was tested in a bulk assay. As depicted in The invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The foregoing embodiments are therefore to be considered in all respects illustrative rather than limiting on the invention described herein. Scope of the invention is thus indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Classifications
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text:
Class speakers Bob Dahl, Mary Lucas Filkins, John Werner, and Shirley Mittendorf
Fifties friends meet
Class speakers at the OHS
multi-class reunion dinner at the Best Western Lodge at River's Edge Conference
Center last Saturday included Bob Dahl, OHS '57 a retired banker from San
Francisco, Mary Lucas Filkins OHS '54 retired teacher from Allyn, WA, John
Werner, OHS '55, retired printing consultant from Scarsdale, NY, and Shirley
Mittendorf LeBlanc, OHS '56, travel agent, from Oak Harbor, WA.
They were among the 200 plus
classmates, spouses, and teacher-guests for the three-day event, including
coaches, Monk Montgomery and Art Neumayer, plus music teachers, Wally Schenk and
Woody Bausch.
Chair of the reunion planning committee was Merle Bobbitt Loseth, OHS '53, who convened the meeting and also spoke.


text:
Sex With Stripper Condom Broke Do I Need To Get Tested For AIDS
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The Robert James Frascino AIDS Foundation
...Hi,Your condom was in the car for two months? Wow, that's quite a dry spell, dude. Do you happen to resemble Dick Cheney or something like that?Your
Read more »
hiv anal sex condom brokeI just recently had anal sex where I was the bottom and I didnt realize the condom had broke until the top pulled out. but by then he had already cum. I'm not sure if the top is pos or neg but he claims to be neg (last tested 4 months ago). What...Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation
...Hello,The CDC estimates the per-act risk for acquiring HIV from unprotected (or broken-condom) receptive anal sex with a partner confirmed to be HIV positive is 5 per 1,000 exposures. Of course there are many confounding variables that can
Read more »
Condom Broke... Sexual Partner tested PositiveDr. F,I practice safe sex all the time. I had anal sex with this guy Jan 1, 2009. (me being the top) the condom broke towards the end before the orgasm. The guy sent me a message telling me he tested HIV+ a week ago. I got so worried and got a blood...Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation
...Hi,Condom failure during anal sex does place you at some degree of risk for STDs, including HIV. Consequently testing outside the window
Read more »
Safe Sex and HIV (CONDOMS) (CONDOM EFFICACY)Hello Dr. Bob. How are you doing? I was just wondering about something. Has there ever been a CONFIRMED case of someone using proper protection and still contracting HIV? I know you say that if a condom doesn't break and is used properly then the...Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation
...answer to how often I would recommend that you be tested would depend on a variety of variables. But remember, the option of testing is always open to you if you feel there has been a potential exposure. Yes, information is indeed power. Stay...
Read more »
South Africa: Condoms Star in a Sex Film With a Message... Filming of South Africa's first all-black pornographic movie has been completed, and condoms have a starring role. Tau Morena said the film -- entitled "Mapona," or "naked" in the Sotho language -- has "a gentle message Read more »Swaziland: AHF Launches Free Condom and HIV Testing Campaign in Manzini...HIV/AIDS medical care and lifesaving antiretroviral treatment, kicks off ambitious "Love Condom" campaign and stepped-up free HIV testing effort in hard-hit African nation. Swazi group produces four compelling safer sex print ads promoting... Read more »Activist Urges AIDS Tests, Condoms in Shelby County Jail...meeting on Wednesday, longtime activist Novella Smith Arnold urged the county Sheriff's Office to allow jail inmates access to condoms and require they be tested for HIV. "AIDS is still alive and well," Arnold told the commission's law... Read more »Understanding the Dynamics of Condom Use Among Female Sex Workers in China...study examines "the complex relationships between multiple levels of determinant factors of condom use with clients among female sex workers in China" through the use of "a hypothesized theoretical model that combines concepts from the health... Read more »
Connect With Others
anal sex -- condom broke
Posted by Anonymous, 2 Replies
...him, with me penetrating him. After some drinks we turned down the lights and started. After some thrusts, I pulled out and the condom was torn from one end to the next. I quickly went to the bathroom and washed my penis with water and soap. That...
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Posted by Anonymous, 2 Replies
...What are the chances of catching hiv when: You have sex with a male with the condom on, after he cums he pulls the condom off and you play around for around 10 minutes he puts his penis
Read more »
Condom Broke Anyone?
Posted by Anonymous, 10 Replies
...Has anyone heard of someone getting infected from a single incident where the condom broke and you were the receptive partner in anal? I know it can happen, but is it something that happens alot? I need some
Read more »
Posted by Anonymous, 2 Replies
...do latex condoms really prevent the tranmission of HIV? Is it recommended for people who have sex with a condom get tested after the sex? How long would they have to wait to test? Is Home Access reliable?
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S/O of is it ok to leave her 1 year old in the tub
Posted by
+ Show Post
Replies (21-28):
Silver Member
on Nov. 28, 2012 at 9:45 PM
Eh, i let my kids play n the tub alone. I can see them the whole time. They are 5 2 N 14 months
by Anonymous 4
on Nov. 28, 2012 at 9:46 PM
I hear everything in there. My kids are loud in the tub. :)
Quoting Anonymous:
I'm too worried I wouldn't hear, such a pointless risk.
Quoting Anonymous:
Yes I heard the fall.
He was holding his head up.
Quoting Anonymous:
Did you hear baby fall, were they under?
Quoting Anonymous:
My 2 yo slipped in the tub once. I was there in 5 seconds, from my room. All was good.
on Nov. 28, 2012 at 9:47 PM
I have a two year old, and I am always with her. I have no idea when she will be old enough to bathe on her own, though. Nobody answered that question in the last post, either. I guess it may be different ages for different kids or maybe nobody wants to be judged. If it's the latter, just PM me.
Posted on CafeMom Mobile
on Nov. 28, 2012 at 9:49 PM
My 5 year old takes showers by himself and started at 4 but any younger and I'm there. My 2 year old and 7 month old are not ever left alone.
Posted on the NEW CafeMom Mobile
Misha Lovegood<3
on Nov. 28, 2012 at 9:49 PM
I wasn't comfortable leaving dd until she was almost 5. I just wasn't.
Posted on the NEW CafeMom Mobile
Platinum Member
on Nov. 28, 2012 at 9:49 PM
it COULD happen when you are there, however if you are right there paying attention as he slips and falls you can catch him before he drowns
on Nov. 28, 2012 at 9:50 PM
My parents neighbors granddaughter died from that :( it was soo sad, the other grandmother left her and went downstairs on the phone while she was babysitting and her 8 year old aunt found her, she was on life support but sadly she didn't make it. It was just in the paper last week that they're charging her with manslaughter. I believe she was 13 months
Posted on CafeMom Mobile
Bronze Member
on Nov. 28, 2012 at 9:53 PM
I do not leave my 2 year old alone in the bath. If I am busy he takes a shower with his older sister or older brother. Too risky
Posted on CafeMom Mobile
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Interval HD Helps Members Channel Vacation Possibilities
Interval International, a prominent worldwide provider of vacation services and an operating business of Interval Leisure Group (Nasdaq: IILG), launched Interval HD, an online video channel featuring approximately 50 destination and resort videos for member families. This dynamic vacation planning tool capitalizes on consumers' increasing usage of video in most every aspect of their lives. In fact, Google statistics revealed that 46 percent of personal travelers watched travel-related videos in 2011, an increase of 27 percent in the past two years.
Videos currently featured on Interval HD include destination overviews showcasing popular locations such as Orlando, Las Vegas, and Cancun. In addition, resort features focus on some of the most desirable vacation experiences available through exchange, a benefit that gives members the ability to trade their resort week or points for comparable accommodations in the Interval network, and Getaway vacation rentals.
"When making their vacation plans, Interval members want as much information about the resort and destination as they can find," said Sharon Freed, Interval's senior vice president of consumer marketing. "Now, Interval HD takes information access to the next level. Members can further sharpen their image of a dream vacation, and more easily put themselves in the picture."
The videos, approximately one-to-three minutes in length, are a click away on IntervalWorld.com. To view them, members access the Interval HD landing page and choose which destination overviews and resort vignettes they wish to see, or they can visit the online Resort Directory.
Plans are to add a minimum of 30 more videos later this year, in addition to those that will be supplied by Interval-affiliated resorts interested in ramping up exposure of rental and sales opportunities. "We've created this enhanced content to assist our members in making vacation plans when using any of our programs. But we also feel it will help resort clients using multimedia presentations at their sales tables to bring to life the benefits of exchanging to a broad range of locations," Freed concluded.Check Out Our Best Services for Investors
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Sigmund Freud, James Jackson Putnam and the Purpose of American Psychology
Winner of the 2007 Gradiva AwardAn innovative work of biography that traces the lasting impact of the friendship between Sigmund Freud and pioneering American psychologist James Jackson Putnam. In 1909 Sigmund Freud made his only visit to America, which included a trip to "Putnam Camp"–the eminent American psychologist James Jackson Putnam's family retreat in the Adirondacks. "Of all the thingsThe war has turned but the Nazis can still unleash Hell
1943. On the Eastern Front the Germans are losing the battle; to the west, they are assailed by the Allied Powers. But in a snow-clad valley in the Hartz mountains of central Germany, a British agent observes the visit of a senior Nazi to a top secret location. That Nazi is Hermann Goering and the Reichsmarschall does not have surrender on his mind. James Jackson comments: 'It was a moving andTo kill the Nazi monster best cut off the head
1942. The war grinds on, losses mount, and the Allies search for a magic bullet that will bring a swift conclusion. When their intelligence services are approached by a senior officer of the German Abwehr, a process begins that could shatteringly alter the course of history. James Jackson comments: 'While visiting locations linked to Operation Foxley - the embryonic British intelligence plan toJie's World (Terran Chronicles)
Terran Chronicles Short Stories (Book #3)
This is the third short story belonging to the Terran Chronicles Universe. It follows Jie as fate leads him far out into space, and toward the very edge of the Milky Way. Jie is under pressure from the Chinese President's son, Wu, to steal the ship. Wu wishes to return to a time of empires and dynasties with Jie being his second in command. In a bold move, Jie steals the ship, fires nuclearGamin Technology (Terran Chronicles)
Terran Chronicles Journals (Book #1)
This journal contains Joe's notes of his discoveries and observations during the Terran's maiden voyage of exploration to the stars. This journal is not a stand-alone story, but rather a supplementary guide for those that have an understanding of the Gamin and their involvement with Earth. While Joe's journal answers many questions about these aliens and their technology, it also manages to raiseTerran Chronicles Short Stories (Book #2)
This is the second short story belonging to the Terran Chronicles Universe and follows the intrepid crew of Artemis One. The four men return home, to Pythos, ending a three year space mission. They enter orbit in time to witness an apocalyptic battle. Hundreds upon hundreds of small craft swoop at a fleet of massive ships. Flying through the midst of the battle, the crew is helpless to do anythingThe Progressive Squeeze: Jobs, Economy & Laws
The Progressive Squeeze: Jobs, Economy, Laws, ObamaCare, Racism & Crime by attorney James A. Jackson is a must read before the USA 2012 elections.You can use this book in a serious but fun way to discuss with your kids (middle school and older) the USA elections in 2012, politics in general and the important things in life. (View it on a Kindle Fire or I-Pad as many of the pictures are in color.


text:
Winnipeg Free Press - PRINT EDITION
BlackBerry back to normal, top execs finally go public
BLACKBERRY services returned to normal Thursday after four days of global outages, but the maker of the popular smartphone faces a new set of challenges as it tries to clean up a public relations headache.
Research In Motion's two co-CEOs -- Mike Lazaridis and Jim Balsillie -- emerged from more than three days of silence to answer questions and apologize again about the biggest outage in the Canadian company's history.
As the troubles wore on this week, RIM (TSX:RIM) offered only a few updates on what was happening as a growing number of BlackBerry users turned to their Facebook and Twitter accounts to express their frustrations.
The Waterloo, Ont.-based company has about 70 million BlackBerry subscribers around the world, and though the technical glitches did not affect everyone, the word of discontent spread fast.
RIM has now stepped into territory where many other major companies have trod in recent years, commonly known as "crisis management" in the public relations industry. It's caused by an event or series of events that could potentially prove disastrous to the organization, if fumbled.
Co-CEO Balsillie defended RIM's decision to wait until the problems were nearly fixed before coming out publicly in a bigger way. He said the company will now turn its attention to addressing BlackBerry users.
"It's a priority, but I do want to say our priority right up until this moment was making sure the system was up and running and operating globally." Balsillie was speaking on a conference call with Lazaridis, a relatively rare appearance of both executives together.
That attitude may have worked in the past, but with the rise of social media in recent years, the urgency of major companies addressing their customers' concerns has reached a new level, some observers say.
"It's important for brands to be perceived as being responsible and perceived as if they care very much about the brand community," said Sidneyeve Matrix, a professor of media and mass communications at Queen's University in Kingston, Ont. "The damage that can be done to the brand is something worth worrying about."
RIM sent out its chief technology officer, David Yach, to explain the problem on Wednesday, the third day when the outages went global. It wasn't until the outage was basically resolved on Thursday that Lazaradis went on YouTube to give an update.
The scripted video, in which Lazaridis spoke directly to the camera while wearing a black polo shirt emblazoned with the BlackBerry logo, was the first instance in which one of the company's co-CEOs appeared publicly to address the outage.
The two executives then quickly scheduled a conference call that was accessible to both the media and the public through the company's website.
Balsillie assured that the outage had nothing to do with the company scaling back its workforce. RIM recently cut more than 2,000 jobs.
The length of the problems has bruised Research In Motion's reputation for reliability and could give consumers and businesses another reason to switch to Apple or Android devices.
RIM had initially told customers its systems were back to normal after an outage affected Europe on Monday, but instead the problems spread to other countries and eventually most of the world.
RIM has long been criticized for keeping customers in the dark over the status of its technical problems, but the dual video and conference call marked a significant change in the way it operates.
"We know that you want to hear more from us and we're working to update you more frequently through our websites and social media channels as we gather more information," Lazaridis told users in the YouTube video.
Shares in Research In Motion closed down 15 cents to $24.12 Thursday on the Toronto Stock Exchange.
RIM rival Apple has become the world's second most valuable company, in part because of its excellent technology, masterful global marketing and strong brand awareness that makes its products a must-buy to millions of consumers.
Apple's success and mounting competition in the consumer tech market has put intense pressure on RIM's business and led to calls from some shareholders of the Canadian technology icon to sell the company or split it up.
In Thursday's conference call, RIM explained that the widespread outage was caused by a technical failure and a backup-system failure in Europe, which caused a massive backlog of emails and texts around the world.
The outages then spread to the Middle East, Africa and hit Canada on Wednesday. Parts of South America, as well as Asian markets, including Hong Kong, Japan, Singapore and India, were also affected.
Lazaridis said Thursday he wanted to apologize to BlackBerry customers.
"All the services are back up globally," he said. "We are and will take every action feasible to address this quickly, efficiently and to minimize the risk of something of this magnitude happening again. "
-- The Canadian Press
Republished from the Winnipeg Free Press print edition October 14, 2011 B4
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Good at Handling Your Money? You're Probably Good at Health Care Too
NEW YORK (TheStreet) -- Did you know that contributing more cash to your 401(k) plan could lead to better physical health outcomes?
Conversely, not contributing to your retirement could be a harbinger of depressed health, says one academic study.
The data come from a study called Healthy, Wealthy and Wise: Retirement Planning Predicts Employee Health Improvements from the Olin Business School at Washington University in St. Louis.
Lead researcher Lamar Pierce, a business professor at the university, found that "poor physical health and financial health are driven by the same underlying psychological factors." That suggests the better the financial decisions you make, the healthier you'll become.
Also see: You'd Better Be Ready for a 40-Year Retirement Pierce and his team tracked employee decisions to contribute to a 401(k) plan and matched those contributions levels to employer-sponsored health exams for employees. First, study participants were given a full health exam, then were provided thorough information on risky heath habits. Researchers tracked the employees for two years, focusing on key financial decisions such as 401(k) contributions and how those decisions affected their personal health. What the researchers discovered should be posted in the break rooms and cafeterias in workplaces across the U.S. "We found that existing retirement contribution patterns and future health improvements are highly correlated," Pierce says. "Those who save for the future by contributing to a 401(k) improved abnormal health test results and poor health behaviors approximately 27% more than non-contributors." Also see: 4 Summer Steps to Personal Wealth (Including Starting That Business) The Washington University team also found that having "insufficient retirement funds" was a solid indicator of relatively poor personal health, and that any decision by an individual employee to pass up a chance to contribute to a 401(k) plan was a predictor of poor health habits. The study results reveal a clear pattern of strong health related to those 401(k) contributions, and poor health after not contributing to a 401(k) -- even if both employees had similar health testing results before making their financial decisions. "After controlling for differences in initial health, demographics and job type, the researchers found that retirement savings and health improvement behaviors are highly correlated," the study noted. "Those who had previously chosen to save for the future through 401(k) contributions improved their health significantly more than non-contributors, despite having few health differences prior to program implementation."Check Out Our Best Services for Investors
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From the Shop ↷
GrayNow for Part II: Don't you usually change out of your New Balance running shoes before going to dinner? There are better understated sunglass choices for everyday use than sport shades. If TTH (Trying Too Hard) fashion sunglasses make you feel self-conscious (and hopefully they do), try classic, ophthalmic styles from mid-20th Century (see our sunglasses channel for examples). Too often, guys today, even well-dressed guys, have only one pair of sunglasses or several pairs of sports sunglasses. Ironically, when men get dressed, they make sure their outfit works together and is appropriate for whatever function they're attending. Then they put on our only pair of sunglasses and get into the only car they own (or lease) -- the two weak links in how most men are showing up.
Gray sunglass lenses reduce light intensity without altering the color of objects. Thus, they provide the most natural color vision. Gray is the most popular sunglass lens color in the United States.
Brown lenses enhance contrast by blocking a larger percentage of blue light than gray lenses do. Brown lenses provide a warmer appearance to colors and make greens more vibrant.
Green sunglass lenses provide a cool, soothing tone to colors. Though not as popular as gray or brown lenses, green lenses are often used to create the classic look of aviator-style sunglasses (Ed. note: "Green" today is actually grey/green or G15. You may want to consider bottle green popular during the 1950s/60s).
Amber sunglass lenses block all or nearly all blue light for superior contrast. Amber-colored lenses are frequently referred to as "blue blocker" lenses or "shooter's sunglasses." (Amber-colored lenses are popular among hunters because they provide enhanced contrast when looking at birds and clay targets against the sky.) Though these lenses enhance contrast, they also distort colors and may not be suitable for driving and other activities that require accurate color recognition.
Pink, Orange, Rose and Blue
These and other vibrant colors are available for fashion sunglasses. These lens colors can cause severe color distortion and are unsuitable for driving and other tasks when color recognition is important.
Put this all in a warmed glass and stir until the honey dissolves. Garnish with a twist of lemon.
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