text:
"A great companion"
I really enjoyed reading this book years ago and having grown tired of listing to the radio have taken to exclusively listing to audible books while driving to and from work. I now relish the traffic and find myself sitting in the driveway for a few more momments when i get home to continue to listen. The Shipping news is a favorite of mine and i greatly enjoyed listening to the narration. Paul Hecht did a fantastic job; bringing the characters back to life for me. So far it is my favorite audiobook.
I would compare the Shipping News to anything by John Irving, Anne Tyler, Amy Tan. All favorites. the stories that encompass a lifetime and all its events are the best to me. Full character development, historical references, and characters so rich and deep. They are my companions of the road. A true travel companion that keeps you entertained and removed from the mondane while reminding you that life is more complicated and deeply enriched.
Quoyle - Paul has gripped the vulnerability of the character without making him a sap.
"A reader's jigsaw puzzle."
The most enjoyable part is getting to live out the book again. Hearing the emotion in Joe's recitation that I have felt each time I have read the book. Tt is like getting to relive favorite moments in your life.
Many come close. but no one can really touch this book. It is an all time favorite of mine.
Everything but Owen's voice. He does a great job of bringing the characters to life.
John Wheelwright. I think his views and inspiration for his actions would make a terrific evenings worth of conversation. I first read this book in the early 1990's when i picked up a copy that had been left in the vacation home of a fried. the vacation home was in New Hampshire. I lost myself in the story and as i read the book found a kindered spirit in John and know him like i know myself. A true friend who i would gladly spend any amount of time talking with about the world and all its stuff.
Ok, let's say that we can all agree that Owen's voice would be difficult for anyone to emulate. However, I have to say that while Joe Barrett did a great job on all other characters, because 'THE VOICE' in my head was so different from what he provided it was the one thing about this audiobook that I would change.


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Related Trips (3,759)Sacred Valley
More Sacred Valley Trips-
In the land of the Incas, we journeyed from Lima to Cusco, the Sacred Valley, then to Machu Picchu.
A Travel Blog by tumi1213
... reading about our trip to Machu Picchu. We started in Lima, flew to Cusco, and since it's at 11, 000 ft altitude, chose to start driving through the Sacred Valley on our way to Machu Picchu. Beautiful scenery, great food, and terrific people!
Related Entries (8,114)Sacred Valley
More Sacred Valley Entries-
Sacred Valley of the Incas
A Travel Blog entry by ozzy1180 from Sacred Valley, Peru
... taken down yet. We filled up on a great variety Peruvian foods. Back in the shuttle and headed to Ollyantaytambo, also a part of the Sacred Valley. We get there around 4pm and drive through the old Inca city. The streets are so tiny, the same as they ...
Amerys travels from the Sacred Valley, Peru
A Travel Blog entry by amery from Ollantaytambo, Sacred Valley, Peru
... Cusco we raved about, but this one is very modern indeed, except for the preserved church in its grounds. http://www.aranwahotels.com/sacred_valley_hotel.php It was simply amazing, and once again someone had been pulling a few strings, as we had this ...
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Tambo del Inka, a Luxury Collection Resort & Spa Urubamba
Av. Ferrocaril s/n, Urubamba, Sacred Valley, Peru
Situated between the ever present voice of the Vilcanota Riverand the stoic silence of the mountains of Cusco's Sacred Valley,Tambo del Inka, a Luxury Collection Resort & Spa, is located in theSacred Valley of Urubamba.The hotel is located 60 minutes ...
Traveler Rating- 1306 reviews
- 8 travel blogs

subset: commoncrawl


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The ESOMAR researcher paper archive is now open!
Members can sign in now for free access to the ESOMAR research paper archive
Visual | Description |
Visual | Description |
ESOMAR's Research Papers are conference white papers from world's leading marketing, advertising and market research professionals. ESOMAR members have full and free access to all papers over 18 months old. To access your archive of conference papers head over to MyESOMAR. If you are interested in becoming an ESOMAR member contact our membership team at firstname.lastname@example.com. EUR 15.00 |
13.05.2014 Running Research Communities in Asian Markets Online research communities are the new star in market research. These online platforms connect a company with a group of interested and interesting consumers to collaborate with in qualitative... |
September 2013 Think Big and Connect to the Max PepsiCo wanted to (re)connect the Ruffles brand with Turkish youngsters. For six weeks a market research online community (MROC) was the central hub in which a dialogue took place between Gen Y, the... |
June 2013 Inspirational Customers Dialogues The annual catalogue is IKEA's main communication channel with existing and potential customers globally. This case study demonstrates how the 2013 edition of the catalogue was evaluated through... |
17.03.2013 In a research world where questionnaires of more than 20 minutes are considered to be 'not done' and consumers are increasingly less inclined to participate in research projects, it is... |
08.11.2012 Recession can prompt unusual levels of creativity. We need to do more with less. Researchers need to increase their business impact. the presenters argue that the way to reach this for qualitative... |
13.09.2012 Designing the Club of Tomorrow The global design project of Heineken, 'Open Design Explorations Edition 1: The Club' invited talented emerging designers from around the world to co-create the club of tomorrow. A three-week Market... |
13.11.2011 Generation Y (aged 15-30) is the most marketing savvy generation ever. On a global scale, this new consumer cohort is much larger than the previous generation X and their impact on society will soon... |
18.09.2011 Great companies understand the importance of consumer and market insights. Enhancing capabilities like acquiring new techniques and technology for data collection and analysis are important to build... |
18.11.2010 How fans become shapers of an ice-cream brand Stimulated by the rise of social media, 'conversational marketing' techniques have gained tremendous importance - a paradigm shift in the way we think about marketing and marketing... |
19.10.2010 Synergizing natural and research communities Research panels are under pressure: response rates are in decline. By using methods like 'social media netnography', researchers can dig into an online source of textual and visual... |


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I am trying to run some javascript code that is created by my codebehind document.
<script type="text/javascript">
function GetMap() {
//some javascript
function AssetDescription(var1, var2, var3) {
//some javascript using var1, var2, and var3
function test() {
alert("test position 1");
var onloadText = document.getElementById("points").value;
alert("after eval");
window.onload = test;
<body runat="server">
<input type="hidden" id="points" runat="server" />
protected void Page_Load(object sender, EventArgs e) {
points.Attributes.Add("value", Iterations());
public string Iterations() {
string toReturn = "GetMap(); \n";
//assetRow is an array of strings. The strings are in the format "var1, var2, var3"
for (i=0; i<numberOfAssets; i++)
toReturn = toReturn + "AssetDescription("+assetRow[i]+"); \n";
return toReturn;
Hopefully I have made all of this as clear as possible. This is all the code I believe is relevant to my question. I am open to other ways of setting this up.
I have tried setting the body.onload element to include my javascript from the codebehind directly, but to no avail. I would like to add this code directly in my tags, but I don't think ASPX has that capability, so this is the workaround I have created.
This seems to be the most promising method i have found. I have searched these forums, and most of you say that eval() is a bad idea. Needless to say, it's not even working. But I would love a more robust alternative if any.
The alert("after eval")
alert never fires, but alert("test position 1")
and alert(onloadText)
both fire.


text:
This week, Tony continues his journey into Office 365 and looks at one of the latest features: Office Delve.
In part one of this series I explained my recent experience in changing over to Office 365. I discussed the migration, the familiarity and compatibility Office 365 has with other Microsoft products and groups and how to establish them. In this article I will further explain my experiences in using Office 365 for business use and how it has enabled me to remain mobile. Read more


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Chair Arm Pads, Armrest Covers, Armrests and more: 1-877-401-4645
Standard Neoprene Armrest CoversStandardized Soft Neoprene Armrest Covers. The tether attachment system uses durable grommets for simple attachment and compatibility with most armrests. The soft neoprene stretches to fit. Common Uses: office chairs, wheelchairs, lawn chairs, patio furniture... Pricing is per complete pair.
Memory Armrest CoversMemory Foam armrest covers are an easy way to upgrade chair armrests of many shapes and sizes. Transform hard factory chair armrests into a pleasantly cushioned surface. High Density Memory Foam cushioning provides superior comfort and support. Pricing is per complete pair.
Gel Armrest CoversGel armrest covers are an easy way to upgrade chair armrests of many shapes and sizes. Transform hard factory chair armrests into a pleasantly cushioned surface. Thick gel cushioning provides superior comfort and support. Pricing is per complete pair.
Product is out of stock
Mem II Armrest PadsSuper comfortable chair armrest pads contoured to support each arm with thick high density memory foam. The universal attachment holes include 3.75", 4", 4.75", 5.5", 6" and 6.5" hole set spacing. The black upholstery looks and feels like leather. Pricing is per pair.
Instant Elbow CushioningElbow Friend armrest pads provide instant elbow comfort and arm support. The universal attachment system makes this an excellent choice for all kinds of chairs. Available in a variety of color options.
Neoprene Armrest Covers MLAC1Soft Neoprene Armrest Covers. The tether attachment system is compatible with many chair armrests. Soft neoprene material plus extra foam cushioning. Uses: office chairs, wheelchairs, etc. Pricing is per pair.
Custom Armrest CoversCushion and protect your chair armrests with a pair of custom covers. Size and color options are available. This product is customized for your specifications.
Delmar II Armrest PadsDelmar chair arm pads offer arm cushioning with a slightly concave support surface to cradle the arms. Standard size fits many different office chairs. Pricing is per complete pair. |
Chair Armrest Pads - ApolloThese office chair armrest pads have 3 sets of bottom attachment screw holes: 4", 5.5", and 7" from center to center. Pricing is per complete pair. |
Strap Armrest PadsGeo armrest pads with cinch straps offer excellent cushioning and support for those hard plastic armrests. Pricing is per complete pair.
Gel Armrest Pads - UltimateUltimate Gel Armrests are designed for maximum comfort. 3.5" Wide x 10" Long. The universal attachment holes are compatible with both 4" and 5.5" center to center screw spacing. Pricing is per complete pair.
Centric Chair Arm PadsCentric office chair arm rests offer a unique surface design. These arm rests work with many standard office chairs. The bottom attachment screw holes accommodate both 4" and 5.5" spacing.
Wheelchair Arm Pads - BlackBlack wheelchair armrest pads in Desk Length or Full Length. Pricing is per complete set (right and left) of wheelchair armrest pads.
ErgoRest Arm & Wrist SupportErgoRest is a fully adjustable arm and wrist support. ErgoRest can relieve strain in the neck, shoulder, elbow, forearm and wrist. Built of sturdy aluminum alloy and tough nylon. Leather upholstered pad. Easy install with adjustable clamp.
Chair Armrests - LoopLoop chair armrests are standardized to fit many chairs with this same style. These may be used as replacement armrests. Pricing is per complete set of 2 (right and left).
Chair Armrest Pads - WhaleWith its unique shape, Whale goes great lengths to support your arms. These chair arm pads are 12" long and 3.5" wide. Whale armrests have bottom attachment screw holes that work with both 4" and 5.5" spacing.
Aero Chair Arm PadsThese chair arm pads are very soft and comfortable with an attractive contoured design. The bottom attachment holes are spaced 4 inches apart. Pricing is per complete set (right and left) of chair arm pads.
Chair Armrest Pads - PacificStandard office chair armrest pads. Replace or upgrade existing chair armrests. Top quality polyurethane material. Bottom attachment screw holes are spaced 4" apart from center to center.
Chair Armrest Pads - DuroDuro chair arm pads offer a durable design and medium-firm cushioning. A solid steel core runs most of the pad length. The bottom armrest attachment holes are spaced 4" from center to center.
Chair Armrest Pads - SunsetSunset office chair arm pads are 11" long and have a slightly concave surface to cradle your arms. The attachment screw holes are 4" apart from center to center.
Adjustable Height Armrest PostsThese adjustable height chair arm posts can be used with many different kinds of chair armpads. The arm posts connect to brackets beneath the chair. Pricing is per complete set of 2. Also referred to as "chair uprights" or "chair arms".
Chair Armrest Pads - DiamondOffice chair armrest pads with a touch of style. The smooth cushion surface has a subtle diamond pattern that appears 3-dimensional under light. The attachment screw holes are spaced 4" apart. |
Chair Armrest Pads - GeoGeo office chair arm pads are among the softest that we offer. They are Durable, Comfortable and Economical. Also referred to as office chair arm rests or chair arm cushions. The bottom attachment screw holes have 4" center to center spacing.
Eck Adams Armrests For Stackable ChairsThese armrest pads are compatible with Eck Adams stackable chairs. Pricing is per complete pair (2 pads).
Chair Armrest Pads - Gator IIGator II office chair armrests offer attachment screw holes for both 4 inch and 5.5 inch spacing. These chair armrests are 12" long and 3" wide. Gator II has slightly softer cushioning than Gator I.
Wool Wheelchair Armrest CoversMediWool armrest covers offer cushioned comfort for wheelchair users. These also work great for some office chairs. This plush Merino wool provides an extremely soft surface. Dimensions: 18" long x 8" circumference, closes with velcro.


text:
About Group
No activity in last 7 days
Group Description
we are a group of like minded people at the Yale University who are committed to work towards animal welfare. We also believe in spreading awareness on issues related to all animals, whether they be wild, domestic, commercial or pets.
Group Information
- 13
- Yale University
- Sep 21, 2003
- English
Group Settings
- This is a restricted group.
- Attachments are permitted.
- Members cannot hide email address.
- Listed in Yahoo Groups directory.
- Membership requires approval.
- Messages are not moderated.
- All members can post messages.


text:
Content Analysis
See how 247dy.com describes itself, and other content related analysis like family-safe content, language of the website etc.
Title: | 34 theater - the latest TV series for free - Baidu video free baidu movie site |
Description: | Baidu audio and video online watch 34 movies net for everyone, movie downloads, the latest drama, nice fast broadcasting of films and television shows for the purpose of audio and video 34 cinema is the online home of movie fans, the update is very fast, users favorite fast broadcast Movie site! ! ! |
Keywords: | Movie heaven, free movies, latest movies, Baidu video, fast broadcasting |
Tags: | Movie Heaven, Baidu Video, Free Movies, Latest Movies, Fast Broadcasting |
Language: | Chinese |
Family safe content: | Yes |
Malware detected: | No |


text:
Discussions not related to Steelers Football
Discuss all non-Steelers related NFL stuff here!
Talk about everything and anything college football.
Our guesses as to how the first round of the Draft will play out! Submitted by our very own LLT!
V3 Mock date: April 27, 2014
Draftniks rejoice! Talk about all things related to the NFL Draft.
For you fantasy geeks out there!


text:
The requested ad could not be found.
Recent apartments/houses ads.
$2,380, 2br, Remodeled Condo 2 Beds + 2 Baths Sherman Oaks
(Sherman Oaks)
Remodeled spacious 2bdrm/2bath (1,385 SF) detached unit which feels like a single family home within a large luxury condominium complex; with lots of green and privacy; without side adjacent neighbors; enormous living room; Italian designer porcelain tiles throughout the whole unit; heated...
$1,120, 2br, Great Apartment homes in Ontario
(Vinyard and 4th Street)
$1,120, 2br, Mountain Manor Apt. en Ontario
(Vinyard and 4th Street)
$1,500, 3br, Nicely situated on a corner lot with ample parking.
(Oxnard, CA)
Nicely situated on a corner lot with ample parking. This one story property features 3 bedrooms, 2 bathrooms and an enclosed patio. The master bedroom has its own private bathroom. Attached garage with laundry hook up's and direct access into the house...
$600, 1br, Best Location In Barstow!! Available Now
$600, 1br, 1 Bd/ 1 Ba Move In Special Call Today!!!
$1,100, 3br, Three Bedroom Apartment in Pacific Palisades, Only $1,100/Month
(Pacific Palisades)
An amazing three bedroom apartment has just been made available for rent in the Pacific Palisades section of Los Angeles for the low and affordable monthly rental price of only $1,100/Month, including utilities. This apartment is near Sunset Blvd & La Cruz...
$1,120, 2br, Affordable Two Bedroom Near Public Transportation, Shopping and Dining
(Vinyard and 4th Street)
$1,120, 2br, Apartamentos Asequibles En Ontario
(Vinyard and 4th Street)


text:
Recent Comments
Recent Posts
- in verse #50 : In voiceless text
- "Dean Millman, Artist"
- 2014 Mormon Literature Year in Review–Part 2, Mormon and Independent Markets
- In Tents #50 This Jesus Whom Ye Slew and Other Texts That Don't Behave Part II
- The Business Side of Writing: "Making It"
- 2014 AML Awards Finalists
- The (New) Amateur World of Writing and Publishing
- In Memoriam: Lu Ann Brobst Staheli
- 2014 Whitney Awards Finalists
- LDS Fiction: Changing Paradigms, by Pam Williams
New Reviews
- Stevenson, "For the Cause of Righteousness: A Global History of Blacks and Mormonism, 1830-2013" (reviewed by John Baucom)
- Holland, "To My Friends: Messages of Counsel and Comfort" (reviewed by Trevor Holyoak)
- Skousen, "The Naked Communist" (reviewed by Michael J. Thompson)
- Lund, "Fire and Steel, Volume One: A Generation Rising" (reviewed by Denice Stiles-Mounce)
- Nelson, "Moroni and the Swastika: Mormons in Nazi Germany" (reviewed by Andrew Hamilton)
- Skousen, "The Real Story of Christmas" (reviewed by Elizabeth W. Roach)
- Bytheway, "Embark!" (CD reviewed by Elizabeth W. Roach)
- Berlin and Brettler, eds., "The Jewish Study Bible: Second Edition" (reviewed by Greg Seppi)
- Silva, ed., "New International Dictionary of New Testament Theology and Exegesis (4 vols. + index vol.)" (reviewed by Bryan Buchanan)
- Dobson, ed., "NIV First-Century Study Bible" (reviewed by Les Blake)
Category Archives: The Past through Literature
in verse #48 : Voice of the turtle
That title is not a reference to Mitch McConnell, no matter how much people say he resembles a turtle. No, it's a reference to "Canticles," a book of the Bible hitherto unknown by this moniker to me, but familiar to … Continue reading
Posted in In Verse, Literary Views of Scripture, The Past through Literature
Tagged Betsy Urkkila, Brion Gysin, Collage of myself, Dean C. Jessee, Kenneth Price, Leaves of Grass, Matt Miller, Personal Writings of Joseph Smith, Sean Cole, Studio 360, Walt Whitman, Whitman the political poet, William Blake
2 Comments
in verse #46 : Filthiness, flood wood and rubbish
Alright, so I've talked about the text of Joseph Smith's letter from Liberty Jail for two posts now, and I'm still not done. But, I hear you saying, haven't you made your point? Well, obviously not, or I wouldn't be … Continue reading
in verse #45 : The Power of the Editor
The text of his letter from Liberty Jail was published in Joseph Smith's lifetime, in Times and Seasons in May and July of 1840, of which Joseph was nominally editor (this was the last transcription Joseph could have overseen). It … Continue reading
in verse #40 : owed to Keats
Have you ever wanted to correct a classic of literature? One that makes an egregious error, but that can be easily corrected? Like, say, this: On First Looking into Chapman's Homer Much have I travell'd in the realms of gold, … Continue reading
Posted in In Verse, Mormon LitCrit, The Past through Literature
Tagged Balboa, Christopher Hill, enclosures, English literature in history. 1780-1830 : pastoral and politics, God's Englishman, John Keats, loss of commons, Ode on a Grecian Urn, Oliver Cromwell, On First Looking Into Chapman's Homer, Roger Sales, stout Cortez
2 Comments
In Tents #33 More on Figurative Language and Interpretation (Who You Callin' a Moron?)
September, the beginning of school, thirty-seven years since I signed on with the BYU moving crew to move the books into the new library. That was back when the new library was not the addition under the quad between the … Continue reading
Writing Church History
A few weeks ago, I saw someone post a comment on Facebook about "the suits" in the Church History Library and how they tell Mormon history. About the same time, I officially became one of those suits: I now commute … Continue reading


text:
The 2015 Corvette Z06 was introduced at the 2014 North American International Auto Show back in January which seems like a lifetime ago. But on Monday, a bevy of auto magazines finally released their long anticipated reviews of the most powerful and track capable Corvette that Chevrolet has ever produced.
One of reviewers of the new 650 horsepower super car was Motor Trend's Carlos Lago who filmed the experience for MT's Ignition webcast which appears on their YouTube channel.


text:
Product Description
Available while supplies last.
The Vernal Falls chandelier collection, by Yosemite Home Decor, embodies the aesthetic features of contemporary artistry with a touch of sophistication. This model's Satin Nickel frame and White Frost glass blend in very well with contemporary cosmopolitan architectural design and easily provides crisp, clean illumination to a small room, a nook, or over the dining room table.
Voltage: | 120 volt | |
Weight: | 12 Pounds | |
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Warranty | 1 year warranty on parts |
Bulb sold separately
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Category | Chandeliers - 89 |
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text:
Went down to Thunder Bay to run the Miles with the Giant Half Marathon. I was so impressed with this hotel.
- exceptionally clean and clean smelling
- wonderful front desk staff on all shifts, very helpful
- gave us free appetizer coupons for the restaurant next door
- quiet hotel
- beautiful little patio out back
- excellent continental breakfast included
- free wifi everywhere in hotel
- off the beaten path, (which is a pro if you are looking for quiet)
- no pool/hot tub.....the hotel staff indicated that they are the quiet hotel - other hotels that have pools have all the sports teams there....so, it is also a pro if you want quiet
I would definitely recommend and stay there again.
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Hotels.com, Expedia, Days Inn Hotels, Priceline, Travelocity, Despegar.com, Odigeo and TripOnline SA so you can book your Days Inn Thunder Bay North reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Days Inn Thunder Bay North Hotel Thunder Bay
- Thunder Bay Days Inn
- Days Inn Thunder Bay North Ontario


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| |||
Ziggy's by the Sea has the following events taking place at the following dates and times. To sort the list, click on the column header. To find tickets for the given event, date & time, click the tickets link in that row. |
Hellyeah, Devour The Day & Like A Storm | Ziggy's by the Sea Wilmington, NC |
Friday 03/06/2015 8:30PM |
view tickets |
The Kooks | Ziggy's by the Sea Wilmington, NC |
Thursday 05/07/2015 8:00PM |
view tickets |


text:
US 5516654 A Abstract A process for increasing the yield of recombinant bone-inducing proteins of the BMP-2 family is provided, wherein dextran sulfate is added to the culture medium in which cells expressing the proteins are grown.
Claims(1) 1. In a process for producing a recombinant bone-inducing protein of the BMP-2 family which comprises culturing in a suitable culture medium a mammalian host cell grown in suspension, said host cell being transformed with a gene encoding BMP-7, and recovering the protein from said culture medium, the improvement for increasing the yield of said bone-inducing protein which comprises adding about 1 to about 1,000 μg/ml dextran sulfate to said culture medium.
Description This application Ser. No. 679,451, filed Apr. 2, 1991, now U.S. Pat. No. 5,318,898. This invention relates to a method for increasing the yield of recombinant bone-inducing proteins of the BMP-2 family. The cloning and expression of the recombinant osteogenic proteins of the BMP-2 family has previously been described (J. M. Wozney, et al., Science 242:1528-1534 (1988); E. A. Wang, et al., Proc. Natl. Acad. Sci. USA 87:2220-2224 (1990), incorporated herein by reference). Osteogenic proteins of the BMP-2 family are a promising development in the bone and cartilage field. The BMP-2 family of proteins includes BMP-3, BMP-4, BMP-5, BMP-6 and BMP-7, and bone-inducing proteins and proteins which are encoded by DNA sequences which hybridize thereto under stringent conditions. The BMP proteins include BMP-1, BMP-2, BMP-3, BMP-4, BMP-5, BMP-6 and BMP-7, disclosed for instance in United States Patents 5,108,922; 5,013,649; 5,116,738; 5,106,748; 5,187,076; and 5,141,905; BMP-8, disclosed in PCT publication WO91/18098; BMP-9, disclosed in PCT publication WO93/00432; and BMP-10, disclosed in co-pending patent application, serial number 08/061,695, filed on May 12, 1993, now abandoned. They may also include other proteins of the TGF-β superfamily of proteins, such as activins. Examples of activin proteins which may be used in accordance with the methods of the present invention is described in co-pending patent application 08/061,464, filed on May 12, 1993, now abandoned. Examples of members of the TGF-β superfamily of proteins which may be used in accordance with the present invention are Vi-1 and VL-1, which are described in copending patent application 08/164,103, filed on Dec. 7, 1993, now abandoned. The methods of the present invention are also useful with heterodimers of the above BMP proteins and TGF-β proteins. Examples of preparation of such heterodimers are described in copending patent application Ser. No. 07/989,847, filed on Nov. 27, 1992. The disclosures of all of the above references are incorporated herein by reference. These osteogenic proteins may be produced in cultured mammalian cell lines by transformation with an expression vector containing the respective cDNAs. The yield of expressed recombinant bone-inducing proteins can be increased in accordance with the present invention by addition of dextran sulfate to the cell culture medium. Genes encoding the foregoing recombinant osteogenic proteins may be expressed in mammalian cell lines such as CHO (Chinese Hamster Ovary), COS, BHK, Balb/c 3T3, 293, and similar cell lines known in the art. The mammalian cells may be grown in any suitable medium, such as e-MEM, Dulbecco's MEM, RPMI 1640, and other media (Freshney, R. I., Culture of Animal Cells, A Manual of Basic Technique, Alan R. Liss, Inc., New York (1983)). The cells may be grown in the presence or absence of a serum supplement such as fetal bovine serum (FBS). The cells may be grown in monolayer or suspension culture, and additionally may be grown in large production scale batches. The expressed osteogenic proteins are recovered from the culture medium and can be purified using known methods. Transformed CHO cells are the preferred host cells used to produce an osteogenic protein of the BMP-2 family in accordance with the present invention. The cell growth medium may be supplemented with FBS to improve the growth of transformed CHO cells in culture. If it is desired to add FBS, concentrations of FBS as low as 0.5% (v/v) may be added. However, addition of animal-origin proteins always presents the risk of harboring viruses and other deleterious agents. The addition of FBS is not necessary for the practice of the present invention. Any dextran sulfate may be used. One example is dextran sulfate of molecular weight 500,000 and sulfur content 17% (Pharmacia). Another example is dextran sulfate of molecular weight 5,000 and sulfur content 18% (Sigma catalogue #D-7037). In accordance with the present invention, the growth medium is supplemented with dextran sulfate at a range of concentrations from about 1 to about 500 μg/mL. Higher concentrations of dextran sulfate work but may interfere with cell growth or protein purification. Preferably, the growth medium is supplemented with about 5 μg/ml to about 50 μg/ml dextran sulfate. Most preferably, the growth medium is supplemented with about 10 to 20 μg/ml dextran sulfate. Expression of BMP-2 can be achieved by inserting a BMP-2 gene into an expression vector, inserting this vector into a mammalian cell, and selecting for cells which express BMP-2. See allowed commonly assigned, patent application Ser. No. 179,100, filed Apr. 8, 1988, now U.S. Pat. No. 5,013,649, the contents of which are incorporated herein by reference. The yield of recombinant BMP-2 protein from mammalian cells which express the BMP-2 gene may be measured by known methods such as radioactively labeling cells with [35 S]- methionine and analyzing secreted proteins by polyacrylamide gel electrophoresis (PAGE) and autoradiography. For measurement of BMP-2 expression from production-scale batches, the amount of functional BMP-2 secreted is preferably quantitated by bioassay. Any appropriate bioassay may be used, for example, assay of induction of alkaline phosphatase activity in a BMP-2-responsive cell line, or assay of ectopic bone formation in a mammal such as rat, rabbit, cat or dog. The optimal concentration of dextran sulfate for increasing yield of BMP-2 was determined by pulse labelling cells with [35 S]-methionine, followed by a four hour chase in varying amounts of dextran sulfate. Total secreted protein was then analyzed by PAGE and autoradiography. Cells were analyzed under two growth conditions: 1) standard adherent monolayers, and 2) suspension and serum free adapted cultures. Two different sizes of dextran sulfate (5000 dalton and 500,000 dalton) were tested for each growth condition. A concentration range of 1 μg/ml to 1000 μg/ml was examined for each size dextran sulfate on each growth state. The results demonstrate that the potency of the 5000 dalton material was similar to the 500,000 dalton dextran sulfate for both monolayer and suspension cells. For cells grown in suspension, 1 μg/ml dextran sulfate produced a small increase in BMP-2 levels. Yield was near maximal at 10 μg/ml, and was approximately three-fold higher than in the absence of dextran sulfate. Increasing the dextran sulfate concentration to 100 μg/ml elicited a further 10% increase in BMP-2 yield. As another example, BMP-2 yield was about 40% higher utilizing 1000 μg/ml dextran sulfate than utilizing 10 μg/ml dextran sulfate. In other words 1000 μg/ml dextran sulfate yielded approximately four-fold more BMP-2 than was obtained in the absence of the compound. While a similar graded response was observed with monolayer cells, the potency of dextran sulfate in increasing BMP-2 yield was reduced about 10-fold. Threshold effects were seen at 10 μg/ml, while levels of 100-300 μg/ml were needed to elicit a near-maximal response. Typically, 100 μg/ml dextran sulfate was used to increase the yield of BMP-2 from cells grown in monolayers. The magnitude of the dextran sulfate effect was about four-fold, similar to that seen with suspension cells. The yield of the other osteogenic proteins, BMP-3, BMP-4, BMP-5, BMP-6 and BMP-7, may similarly be improved with the addition of dextran sulfate to the cell culture medium. Patent Citations
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text: So i am new to this group but am trying to do research for my baby registry. I am wanting a baby carrier that i can carry my baby in both facing me and then when older facing out. The problem i am running in to is that i want something that fits both my husband and dad who are both tall and big guys. My dad is 6'2' and long wasted. We have tried a few and they either hit him wrong on the back or just really don't fit and aren't comfortable. I would love some suggestions.


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Who spiked the punch? Vodka or rum, mixed drinks or shots, get recipe ideas for your next party.
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article to video conversion
Closed - This job posting has been filled.
Job Description
I posted this job earlier and I think my potential applicants got the wrong idea about what I was looking for. If you already applied for this job please re-apply if you are still interested after reading this revision.
I am looking for basic video creation of articles I have written from my blog. Most of them are 1200 to 1500 words and I would like slideshow videos created with voice over. I want the videos to look nice and be informative, but I don't need them to be much more than a fancy powerpoint slide show.
Here is a link to an example of what I need. I would like the quality a little better than this, but this gives you an idea of what i am looking for: http://www.youtube.com/watch?v=WpU
I previously put in my budget that I had $500 to work with, but that was my total budget - not how much I wanted to pay for just one video. I am looking to stay in the $20 - $30 range for each video and I have several videos I would like made.
I am willing to provide work on a regular basis. Please understand, I am not looking for videos that are 10 or 20 hours to create. I just need simple slideshow videos that can be done in an hour or two each.
I will provide you with the article content and I would like an MP4 or equivalent quality video produced.
I also would like a short video intro produced.
Thanks for applying!
Other open jobs by this client
- Fixed-Price – Skull or tattoo Illustrations


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Look here for all kinds of legal information designed for use by regular folks--there's even a dictionary to translate "legalese"
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All the basics about the highest court in the land.
From the Washburn University School of Law Library in Topeka, Kansas.
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Tuesday, 28 Aug 2012 03:40 PM
The worst U.S. drought in more than half a century has sent the cost of corn and other animal feed soaring, and the news from Sanderson illustrates how that will translate into costlier food for consumers.
Shares in the poultry company jumped 9 percent on the strong results and word that it began trimming production by 2 percent earlier this month. The production cut follows a 4 percent reduction put in place in January, executives said on a conference call with analysts.
Lowering production helps support retail prices by limiting supply.
"With considerable uncertainty and a high degree of fear now priced into the market for corn and soybean meal," Sanderson is not locking in long-term prices for grain, Chairman and Chief Executive Joe Sanderson said on the call.
Based on Monday's closing prices, Sanderson's feed grain costs would be $61.1 million higher this fiscal year -- an increase of about 10 percent.
"Our view this morning may be very different from our view a few days from now," Chief Financial Officer Mike Cockrell said, citing volatile external factors such as feed grain costs, market prices for poultry and the overall health of the economy.
Jumbo wing prices averaged $1.59 per pound during the fiscal third quarter ended July 31, more than doubling from a year earlier. Boneless breast prices also rose, but at a significantly lower rate due to sluggish food service demand.
Higher poultry prices helped the company swing to a third-quarter profit of $28.7 million, or $1.25 per share, from a year-earlier loss of $55.7 million, or $2.51 per share.
Sales jumped 22 percent to $624.9 million.
Analysts on average expected earnings of $1.20 per share on revenue of $620.0 million, according to Thomson Reuters I/B/E/S.
Shares of Laurel, Mississippi-based Sanderson Farms leaped 9 percent to $44.25 in afternoon Nasdaq trading.
On the New York Stock Exchange, shares of poultry producer Tyson Foods Inc rose 4.4 percent, while pork processor Smithfield Foods Inc inched up 0.6 percent.
© 2015 Thomson/Reuters. All rights reserved.
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March 27th, 2008, 05:49 PM | #1 |
Livin the American Dream
bought a car
I am looking to buy a car so i can quit driving my bronco everyday and save some money on gas. Let me know what ya got or if ya see something. Also give me some input on the cars I have been looking at. I am looking to spend upto about 1,600 unless i come across a fabulous deal then i might go higher, but i would love to be around 1,000
Cars I have been looking at: 95-99 neons 93-96 escorts/tracera 97-98 escorts 95-99 sunfires/cavaliers 94-97 t-birds w/ 4.6 89-95 t-birds/super coupe w/3.8 i am aware of headgasket issues. Last edited by Silver Bullet; March 31st, 2008 at 11:46 AM. |
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March 28th, 2008, 05:51 PM | #3 |
Stacks bring the awesome
im not gonna lie all those cars suck. girls cars are what your looking at. get a grand am or grand prix. even a olds 88 would be a better car. but nothing comes to mind im in the same boat
March 28th, 2008, 05:52 PM | #4 |
Senior Member
crown vics/ grand marquis/ town car. they tend to be pretty reliable and plentiful. plus they are all driven by old people. they get pretty damn good gas mileage for a v8 like 25-28
March 31st, 2008, 11:44 AM | #7 |
Livin the American Dream
just bought a 94 intrepid fully loaded, 24v 3.5, cosmetic collision damage to pass. rear quarter and door,60,000 miles, interior mint. 900.00 drives awesome getting some new rubber on it today, kuhmo's 355.00 out the door w/ road hazard
March 31st, 2008, 11:53 AM | #9 |
Livin the American Dream
Thread Tools | Search this Thread |


text:
TD-Complete Submission
What to expect when you're expecting (an acceptance letter)
4.12 / 5.00 4,648 ViewsHey everyone!
This is a Robot Chicken styled Flash Collab about Pokemon.
It was animated over the course of a few months by me (kawaiipiranha), and SandwichandCereal.
It's something I wanted to put together for a long time, and I'm glad we finally did it.
You can see more of our stuff on our Youtube Pages (linked on the loading screen... you gotta right click and open in new window for some reason x_x)
I saw Sandwich's Pokemon shorts which inspired me to make my own, and I always thought it'd be awesome to work with him.
Putting this collab together was a wreck because our dimensions and framerates were slightly different, but I got it done, even though I have zero actionscript skill....
ANYWAY, Enjoy :)
**PS, there's a secret on the credits screen if you click the right spot ;)**
It would be better if it were more original.
Especially with the voices and style; not everything has to be Robot Chicken based.
Spend your time making original art...
Spend the time you took ripping punch lines from other mediums and write something original. You say it's supposed to be Robot Chicken styled, but Robot Chicken writes their own jokes and has original material.
Try again. I want to see what you guys can do without using the crutch of things people are already familiar with and can associate with comedy. Cause, you have the potential to be really good.
Two of the best entry level flash animators - now working together.
Beautiful man
The animation wasn't that clear, I couldn't tell what half of the toons were. Plus, the stolen voice dubs just made it seem like you were lazy.


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re: The ATL Thread: Spring Training Edition Posted by lsu31always on 2/12 at 8:15 pm to GaBassFisher92 And most closers tend to have like 3 good years then flame out. IE Soria.Reply Back to Top |
Posted by SouljaBreauxTellEm on 2/12 at 8:27 pm to lsu31always He's not most closers any ways. This isn't a Jair Jurrjens situation. Atlanta is already set up for the now as is. Play to the strengths of having a dominant back end and improve that 3rd base situation. That's just about all you need to do any ways.. Sign a top of the line starter if need be down the road. He is 24 years old. LOL. Again TWENTY FOUR. Reply Back to Top |
Posted by SouljaBreauxTellEm on 2/12 at 8:44 pm to SouljaBreauxTellEm I'll go back and read the article later.. I admit I only read half of it.. but if anything it made all the reasons why the Braves should keep Kimbrel. And now I read a little more. I keep hearing how the Braves payroll is weak and dying down.. yet it continues to grow regardless. Of course any player is a possible trade candidate if it betters the team, but I don't see any return worth it. Not for a closer. Even one as dominating as Kimbrel. Reply Back to Top |
Posted by lsu31always on 2/12 at 8:46 pm to SouljaBreauxTellEm But it is not growing at the rate of other teams. The TV deal is going to hamstring us a great deal.Reply Back to Top |
Posted by Robot Santa on 2/12 at 10:48 pm to lsu31always I hate the concept of a "closer." All it does is give an idiot like Fredi an excuse for keeping his best reliever in the pen in the late innings of a close game, especially a road game. It also allows the guys who get that label to really leverage their way into a huge contract because dumbfrick GMs fap to the idea of "an 8 inning game." Kimbrel should be used to put out a fire late in a 1 run or tie game, not to hold on to a 3 run lead in the top of the 9th. Pigeonholing relievers into one role is fricking stupid and is what has led to a lot of the godawful bullpen management in baseball. As far as my opinion on that article...Kimbrel is awesome. Best relief pitcher in baseball IMO. Still a relief pitcher though. No relief pitcher is worth 8 figures a year. Period. When that money can be used to pay a guy who will pitch 200+ high quality innings or have 600+ high quality PAs, spending it on a guy who pitches 60 innings a year is just a waste. Reply Back to Top |
Posted by NorthGAVol on 2/13 at 8:46 am to Robot Santa Image: http://media.cmgdigital.com/shared/lt/lt_cache/thumbnail/615/img/photos/2013/02/12/33/0f/021312_braves_HS10.JPGImage: http://media.cmgdigital.com/shared/lt/lt_cache/thumbnail/615/img/photos/2013/02/12/2e/9e/021312_braves_HS11.JPG BEAST Image: http://media.cmgdigital.com/shared/lt/lt_cache/thumbnail/615/img/photos/2013/02/12/6d/74/021312_braves_HS15.JPG Image: http://media.cmgdigital.com/shared/lt/lt_cache/thumbnail/615/img/photos/2013/02/12/ea/73/021312_braves_HS23.JPG Image: http://media.cmgdigital.com/shared/lt/lt_cache/thumbnail/615/img/photos/2013/02/12/78/ab/021312_braves_HS14.JPG Reply Back to Top |
Posted by Pilot Tiger on 2/13 at 8:57 am to Robot Santa I agree. Always felt that your best reliever should come in when the situation warrants it. If you're tied in the 7th inning and a runner gets to 2nd, it may be a good time to bring him in. He obviously gives you the best chance to get out of the inning. Reply Back to Top |
Posted by sowega dawg on 2/13 at 9:15 am to Pilot Tiger You get the feeling this outfield is gonna develop a great chemistry while pushing each other to perform night after night. That being said, I believe J-hey has a monster year!Reply Back to Top |
Posted by TH03 on 2/13 at 10:10 am to sowega dawg Our outfield is essentially 3 of the same people. 2 of which are brothers so they're already competitive with each other. This is going to be fun as hell to watchReply Back to Top |
Posted by lsufan1215 on 2/13 at 10:58 am to TH03 quote: Reply Back to Top |
Posted by bamafan425 on 2/13 at 11:10 am to NorthGAVol Image: http://media.cmgdigital.com/shared/lt/lt_cache/thumbnail/615/img/photos/2013/02/12/6d/74/021312_braves_HS15.JPGReply Back to Top |
Posted by au21tigers on 2/13 at 12:01 pm to bamafan425 I want that hat.Reply Back to Top |
Posted by Hurricane Mike on 2/13 at 12:07 pm to volfan30 quote: This is where the economics of baseball has gotten out of control, it doesn't make sense and I'm sure the Braves won't pay Papelbon money to him Reply Back to Top |
Posted by biglil3 on 2/13 at 12:17 pm to Hurricane Mike Heyward looks jacked JUp needs to lose a few Reply Back to Top |
Posted by WicKed WayZ on 2/13 at 2:28 pm to biglil3 Looks like JUP won't be stealing many bases this yearReply Back to Top |
Posted by volfan30 on 2/13 at 3:26 pm to WicKed WayZ quote: Welp. Reply Back to Top |
Posted by RollDatRoll on 2/13 at 3:41 pm to volfan30 No huge shocker there. Losers.Reply Back to Top |
doin drugs n shite Reply Back to Top |
Posted by Porter Osborne Jr on 2/13 at 7:52 pm to beaver Geebus. JHey is yoked. Reply Back to Top |
Not surprised that Schafer is being lazy as frick, dude is worthless, but Pastornicky doing this really pisses me off. Have fun in Gwinnett. Props to Fredi for calling their asses out. Reply Back to Top Refresh |
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US Futures Fall But US Data Tempers Decline
NEW YORK (AP) â¿¿ Investors sent U.S. stocks barreling to their highest levels of the year Thursday, buoyed by slivers of encouraging news about jobs and housing. At least for a day, they overlooked the lack of clarity about Greece's marathon negotiation for a bailout.
The Dow Jones industrial average rose 123.13 points to close at 12,904.08, its third triple-digit gain this year. It was the highest close for the Dow since May 19, 2008, four months before the worst of the financial crisis.
As the Dow moved to within sight of 13,000, applause broke out at the closing bell on the floor of the New York Stock Exchange.
The Standard & Poor's 500 rose 14.81 points to 1,358.04, its highest close in nine and a half months. The Nasdaq composite, which has had an even stronger year than the Dow and S&P and is trading at its highest since 2000, rose 44.02 points to 2,959.85. The rally was broad, with all but one of the 30 stocks in the Dow, Kraft Foods, closing higher. All 10 industry groups in the S&P were comfortably higher, led by materials stocks, including strong showings from DuPont and Dow Chemical. General Motors was among the best-performing stocks of the day. Two years after it was almost wiped out, the company turned a record $7.6 billion profit last year, bigger even than when Americans couldn't stop buying trucks and SUVs. Microsoft rose 4 percent, as did Bank of America, which tends to swing wildly with the market. The Labor Department said weekly applications for unemployment benefits dropped for the fourth time in five weeks to the lowest point since March 2008. That was when the jobless rate was just 5.1 percent, far below the current rate of 8.3 percent. Construction of single-family homes cooled slightly in January, but a rise in permits suggested builders were growing more confident that more buyers are ready to come off the sidelines.Check Out Our Best Services for Investors
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Welcome, Sign In
The Biggest Weight Loss Myth From Joel Harper
Description:Fitness expert Joel Harper discusses what he thinks is the biggest myth about how to lose weight. Part of the series Mondays With Marlo on MarloThomas.com. (0:52)
Source: Mondays with Marlo
Tags: Mondays with Marlo , biggest myth about weight loss , how to lose weight , joel harper


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Be Quiet! PURE POWER L8 500 W
Specifications »Introduction
We would like to thank Be Quiet! for supplying the review sample.
Be Quiet! is probably the largest German company in the PSU and CPU-cooling market. They, as their name implies, mainly focus on providing products with a quiet operation as German users are generally very sensitive to noise, so all of their products are equipped with silent fans of high quality.
The newest additions to Be Quiet's portfolio of products are the fresh L8 units that belong to the Pure Power series. These mostly address the mainstream and mid-end market segments. The new models are six in total, and all of them are equipped with affixed cables to restrict production costs and achieve a lower retail price. We already reviewed the CM L8-730W here, and the L8 ones are also 80 Plus Bronze certified, featuring, according to their manufacturer, exceptionally quiet operation due to the silent 120 mm fan with rifle-bearings. Since Be Quiet! is well known for their low-noise products, we have no reason not to believe them. However, our sound-measuring equipment will be the final judge of that.
Today's menu has as the main dish for the Chromas the Pure Power L8-500W that is equipped with two +12V rails. It can also deliver its full power continuously at up to 40°C ambient, has up to 88% efficiency, and all of its native cables are sleeved in order to provide improved airflow inside the chassis, and to look nice! According to Be Quiet!, this unit is also ready for the Intel Haswell platform, so it supports Intel C6/C7 sleep state energy requirements. We will of course verify or debunk this claim through our tests, especially since it is very hard for budget units to achieve Haswell compatibility as their cost-saving design does not cope well with highly unbalanced loads on all rails.


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Why Adept Technology (ADEP) Stock Is Falling in After-Hours Trading Today
NEW YORK (TheStreet) -- Adept Technology (ADEP - Get Report) was falling -19.4% to $8.97 after-hours Monday after missing analysts' estimates for earnings and revenue in the fiscal fourth quarter.
For the fourth quarter the company reported a loss of -3 cents a share, missing the Capital IQ Consensus Estimate of 3 cents a share by 6 cents. Revenue grew 4.4% from the year-ago quarter to $14.3 million, missing analysts' estimates of $16.22 million.
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Access the tool that DOMINATES the Russell 2000 and the S&P 500.
Product Features:
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David Peltier identifies the best of breed dividend stocks that will pay a reliable AND significant income stream.
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Every recommendation goes through 3 layers of intense scrutinyquantitative, fundamental and technical analysisto maximize profit potential and minimize risk.
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Our options trading pros provide over 100 monthly option trading ideas and strategies to help you become a well-seasoned trader.
Product Features:
- Actionable options commentary and news
- Real-time trading community


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I'm from Belgium and I ordered THE GECKO (it was a Preorder). One week after (on Friday), The gecko was in Stock. MJM has shipped it IMMEDIATELY!...Read More ->
This was my first time buying magic supplies online and I'm very impressed with the service which has gained my trust. If you live outside of America...Read More ->


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What's So Great About the Latest Baby Carriers
Description:We women have been carrying children since… forever. But the way we're carrying them has changed, as in: It's actually become a little easier! (1:31)
Source: NewsFix DFW
Tags: baby carriers , baby tips , baby wraps , how to carry babies


text:
In patients with metabolic syndrome, body iron overload exacerbates insulin resistance, impairment of glucose metabolism, endothelium dysfunction and coronary artery responses. Conversely, iron depletion is effective to ameliorate glucose metabolism and dysfunctional endothelium. Most of its effectiveness seems to occur through the amelioration of systemic and hepatic insulin resistance.
In a study published by BMC Medicine, Michalsen et al. demonstrated a dramatic improvement of blood pressure, serum glucose and lipids after removing 550 to 800 ml of blood in subjects with metabolic syndrome. This effect was apparently independent of changes in insulin resistance, in contrast to previous cross-sectional and cohort studies investigating the association between iron overload, insulin resistance and cardiovascular disease.
Despite drawbacks in the study design, its findings may lead the way to investigations aimed at exploring iron-dependent regulatory mechanisms of vascular tone in healthy individuals and patients with metabolic disease, thus providing a rationale for novel preventive and therapeutic strategies to counteract hypertension.
Please see related article: http://www.biomedcentral.com/1741-7015/10/54 webcite
blood pressure; insulin sensitivity; iron; metabolic syndrome; phlebotomyBackground
Metabolic syndrome (MetS) is a clustering of cardiovascular disease risk factors (for example, visceral, obesity dyslipidemia, hypertension, hyperglycemia, fatty liver disease). Both insulin resistance (IR) and low-grade inflammation underlie the syndrome [1]. The presence of MetS is predictive of type 2 diabetes mellitus (T2DM) and all-cause mortality [2]. In the United States, prevalence estimates of MetS are 33% for the general younger adult population (aged 20 to 59) and 59% in older individuals. In the future, metabolic syndrome may overtake smoking as the leading risk factor for heart disease [3]. Currently, it is possible to prevent or delay the occurrence of MetS, mainly with a healthy lifestyle, which is a lifelong commitment [1].
Other strategies are also possible. Michalsen et al. [4] observed an impressive reduction of blood pressure (about 18 mmHg in the treated group compared with 0.2 in the control group) in patients with MetS after removing 550 to 800 ml of blood. The effect was evident early after the first phlebotomy and persisted two weeks following the second venopuncture (performed at Day 28). The study was a randomized, controlled single-blinded trial of 64 hypertensive patients. Thirty-seven percent had T2DM. Most of them were on drug treatment. Changes in blood pressure and insulin resistance (as estimated by the homeostatic model assessment of insulin resistance, HOMA-IR) correlated with the reduction in ferritin levels.
The beneficial effects of phlebotomies have been repeatedly observed in the past and documented in 1867 by Brunton, who observed that "Small bleedings of three or four ounces, whether by cupping or venesection, were (...) beneficial", and recommended that a few ounces of blood be removed every few weeks from patients with angina. He attributed the relief of angina to the diminution of "arterial tension" [5]. In 1970, Parker et al. [6] observed that angina was relieved and ventricular function returned to normal after a phlebotomy averaging 276 ml. More recently, iron overload was observed to cluster with abnormalities of MetS, including overt T2DM [7-9]. Iron depletion by repeated phlebotomies, erythrocytapheresis or use of iron chelators ameliorated metabolic control, coronary artery responses and endothelial dysfunction [7-9] and the beneficial effects of these procedures on metabolism are thought to be driven primarily by the amelioration of insulin resistance [9,10].
Puzzlingly, the beneficial effect on blood pressure observed by Michalsen [4] was independent of any effect on IR; hence, suggesting an independent mechanism of action. The potential impact of the Michalsen's study is remarkable in terms of reduced health care costs. If effectiveness of repeated phlebotomies in reducing blood pressure in patients with MetS is confirmed, this could dramatically reduce the burden of the syndrome and its related costs. Indeed, phlebotomies as routine means for primary prevention or treatment of hypertension are easy to perform, low-in-cost and, hence, could be a good alternative to more expensive drugs. Moreover, by encouraging blood donation in patients with MetS it will be effective also to respond to the worldwide significant demand for red cells and blood components.
However, the results from this trial generate a number of questions regarding the lack of effect on IR and the early and consistent effects on blood pressure.
Iron overload, insulin resistance and risk of T2DM
A relationship between iron stores and IR has been reported, but with some inconsistencies. The risk of diabetes was significantly lower in frequent blood donors from the Health Professionals Follow-up Study when compared with non-donors after 10 years of follow-up [11]. However, the beneficial effect of frequent blood donation disappeared at 12 years [12]. In the same cohort [12] and in post menopausal women from the Iowa Women's Health Study [13], heme-iron intake from red meat sources was associated with increased risk of diabetes.
Serum ferritin, a surrogate marker of iron status as it accurately reflects body iron stores in healthy individuals [14], was associated with increased risk of T2DM [15]. Subjects with hyperferritinemia had a 2.4-fold higher risk of developing T2DM [16]. This association was further confirmed in other studies [17-19].
None of these aforementioned studies provided information on insulin resistance, which has been explored in cross-sectional investigations. Frequent blood donors had improved insulin sensitivity and decreased insulin secretion when compared with sporadic blood donors or non-donors [20]. In an unblinded and uncontrolled study of 31 subjects with glucose intolerance, serial phlebotomy individually adjusted to induce near iron deficiency was associated with improved insulin sensitivity in response to oral glucose loading and reduced levels of glycated haemoglobin [21]. In another unblinded, randomized study in 28 patients with high-ferritin T2DM, three serial 500-ml phlebotomies reduced mean serum ferritin from 460 to 232 ng/ml and simultaneously led to increased insulin sensitivity and reduced levels of glycated haemoglobin [8].
Concerning the relationship between iron stores, MetS and IR, a number of epidemiological studies showed a relationship [22-24]. Hence, the lack of a significant effect of phlebotomies on IR in the present series is somewhat unexpected. HOMA is a poor predictor of insulin sensitivity in patients with T2DM, but, additionally, more than two phlebotomies and steadily reduced ferritin levels might be needed to see a significant effect of iron depletion on IR in patients with long-term diabetes and on multi-drug treatment as those evaluated in the Michalsen's series [4]. Indeed, significant effects after one year of treatment in high-ferritin T2DM patients [7] and after two years in those carrying hereditary hemochromatosis [10] have been observed. Moreover, drug treatment might have confounded results and thus, it would be important to study in more depth the effects on HOMA-IR by taking into account the use of medication (that is, separating those taking metformin vs. the other groups). Alternatively, the effects of blood donation on IR may differ in subjects with normal versus abnormal glucose tolerance as observed when exploring the effect of iron depletion on brachial artery flow-mediated dilation [25] and in keeping with those of Hirai et al. [26], who demonstrated differential effects of vitamin C in subjects with normal versus abnormal glucose tolerance.
Behind the iron-heart hypothesis
Several epidemiological studies have investigated the effect of iron depletion on atherosclerosis, cardiovascular risk, mortality and morbidity [27]. Blood pressure is one of the main factors driving cardiovascular morbidity and mortality, but no evidence was provided of any reduction in blood pressure following phlebotomies.
By stratifying men from the Health Professionals Follow-up Study according to lifetime number of donations (0, 10 to 20 and ≥30), no difference was observed in the risk for hypertension [11]. The Kuopio Study was the study which reported significant differences in mean blood pressures between donors and non-donors, but such a difference might reflect different lifestyles [16].
Thus, one of the main questions is how two phlebotomies worked to reduce blood pressure. Improvement of IR is not a nominee, at least at a first glance. Michalsen et al. [4] argue that reduced low-grade inflammation and oxidative stress may play a role as iron-dependent hydroxyl radical formation can contribute to vascular dysfunction. In this regard, high-frequency blood donation was associated with reduced serum ferritin and increased brachial artery flow-mediated dilation compared with low-frequency blood donation [22]. Serum ferritin was significantly decreased and flow-mediated dilation was increased in high-frequency donors compared with low-frequency donors with no between-group difference in insulin sensitivity. The decrease in flow-mediated dilation during oral glucose tolerance testing did not differ between high- and low-frequency blood donors [22]. Alternatively, the authors [4] speculate that changes in blood viscosity caused vasodilatation and, in turn, reduced blood pressure.
On the other hand, as blood volume was not replaced following phlebotomies, patients on multi-drug therapy or with type 2 diabetes might also have a dysfunctional endothelium and sympathetic response to relative hypovolemia. They might be unable to compensate for hypovolemia as healthy donors do. Indeed, most previous evidence were from cohort and cross-sectional studies of healthy donors [20], and from high-ferritin T2DM patients and carriers of hereditary hemocromatosis in whom blood volume was restored to normal at each procedure [7,10].
Future directions and conclusions
The strength of Michalsen's study is that for the first time an effect of iron depletion on blood pressure is recognized. Like other evidence from clinical studies, the effectiveness of iron depletion on hypertension needs to be proved with further studies. The focus of the research should move toward two different fronts: i) investigation of the effect of reducing body iron stores through graded phlebotomy, preferably using solid measures of insulin sensitivity, vascular resistance, viscosity and oxidative damage; ii) meta-analysis of data from published cohorts or de novo analyses of much larger cohorts of healthy donors and patients with an appropriately long-term follow-up and tight monitoring of dietary iron intake.
In conclusion, findings from Michalsen's study give new perspectives for prevention and treatment of the metabolic syndrome demonstrating that repeated phlebotomies, a low-cost and minimally invasive technique, are effective in reducing blood pressure with a mechanism that is independent of insulin resistance. Routine phlebotomies in these patients may enormously reduce health care costs related to the epidemic metabolic syndrome and, importantly, also contribute to increase the rate of blood donations.
HOMA: homeostatic model assessment; IR: insulin resistance; MetS: metabolic syndrome; T2DM: type 2 diabetes mellitus.
Competing interests
The authors have no conflict of interest in relation to this work.
Authors' contributions
Both authors contributed equally to the drafting and revising of the manuscript, and both authors approved the final version to be published.
Authors' information
MM, MD Ph.D FACN, is responsible for the research unit for multifactorial diseases at the Scientific Directorate of the Bambino Gesù Children's Hospital, Rome. She has devoted her entire research activity to obesity and type 2 diabetes.
JMFR, MD, PhD, is Chief of the Section of Diabetes. Department of Endocrinology. Hospital de Girona Dr Josep Trueta and CIBERobn. Avinguda de França s/n. 17007 Girona, Spain.
We are indebted to Francesco Equitani for the critical revision of the manuscript.
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Pre-publication history
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So What (CountyJustice)Submitted by MassAppeal (not verified) on Sun, 07/24/2011 - 1:07pm.
So what? Armeria is a lady with great intentions. If you know her, or any of us Grahams, personally, you'd know that we don't bother anyone;in the same token, there is no one that is going to come stomping on our door mats. Now, there are certain ways to handle certain situations, and being that she was put in a life or death situation, she chose life. What's the crime in that? Judgemental individuals, such as yourself cause some others (mostly weak-minded people) to become statistics, with your labeling theories. I assume you're perfect?! A personal philosophy of mine--"I am human;therefore, nothing seems alien to me!" »
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Prisoner Hunger Strike Hits Polk Correctional
On Monday, May 19th, 7 prisoners at Polk Correctional Institution in Butner, NC began a hunger strike in protest of a range of indignities and grievances. According to prisoners in the facility, additional men have been joining the strike since that first day. The strike was initiated in part by prisoners who were transferred out of Central Prison, following a class action lawsuit against the facility for abuse by guards in various "blind spots" around Unit One. That lawsuit has already forced the administration's hand in videotaping any cell extractions by guards.
A demands and grievances list was sent by the prisoners to comrades on the outside. It reads as follows:
1. We are not given brooms to sweep our cells. Provide them.
2. We are denied the ability to exercise proper hygiene by clipping our nails. Provide necessary items.
3. We have to buy a whole new radio just to get headphones. Allow us to buy headphones separately.
4. Our property is not inventoried by staff when they take it. By the time is is, things are missing. Follow proper procedure and let us sign property sheets to verify accuracy of it.
5. Our food trays are melted, peeling, and/or cracked. Plastic from them is regularly found in our food. We need new trays.
6. We do not get outside recreation. Give it to us.
7. Sick calls are done at our cell, eliminating confidentiality. Give them to us in the nurse station.
8. The ventilation system is filthy. Clean it.
9. Our laundry is filthy and rancid, and on top of that we are not issued the proper amount of clothes.
10. Our property box is not 2 cubic feet, but we are still forced to mail anything that does not fit in it home. Allow us what policy states we can have: 2 cubic feet of property.
11. Staff routinely pop doors with no camera or protective gear. They slam our hands in the [food] trap and don't report uses of force. End excessive force.
12. Mattresses and religious items are taken to punish us. Do not take these items.
13. Provide us with pens, forms, hygiene items upon request.
14. SIB (seg) cells are dirty and blood-stained. We are placed in there naked, then only given four squares of tissue to use the bathroom, then nothing to wash our hands, then forced to eat with filthy hands. Clean the cells, and provide enough tissue and soap to properly clean ourselves.
15. The hygiene supplies are insufficient. Provide more when it runs out, or let us buy our own.
16. Toilets only flush twice. At least three are required.
17. The blue razors supplied do not shave properly; we need the black ones.
18. A lot of cells have no stools to sit on at the desk. Give us chairs or something to sit on.
19. STG watch label is given out, then we get treated as if we are actually in a gang. Stop stamping out going mail as STG if we are not in a gang.
20. Our cells are not properly cleaned before we come into them. Mold and mildew in the showers as well.
21. More time than 90 days to write a grievance.
22. A law library.
23. Staff routinely open our trays. Stop picking over our food.
24. We are left in restraints without bathroom breaks or breaks to eat.
25. Stop holding us here (in segregation) for years without write-ups.
26. We need emergency call buttons in the cells.
27. Stop using nutraloaf as punishment. [ed. note: Nutraloaf is an inedible vitamin based, stale brick given out to prisoners instead of real food, often for being rebellious or troublesome. The Supreme Court supposedly prohibits the use of food as punishment in prisons…]
28. Inadequate mental health services for people with mental problems that are worsening.
29. OIC needs to make daily rounds on every block.
30. Superintendant needs to make monthly rounds.
31. Our kosher religious diet is denied here but allowed at other camps. Provide it.
32. Allow us to order religious books that have previously been approved.
The prisoners are encouraging supporters to send mail and make phone calls to:
Frank Perry, Secretary of the Division of Prisons
4201 Mail Service Center
Raleigh, NC 27699
or Call at
(919) 838-4000
sally permalinkJune 5, 2014 1:57 am
If these are true this is. Inhumane.this needs to be taken care before the strike gets any worse maybe Washington needs to be notified.
Becky permalinkJuly 19, 2014 1:04 am
my son was just transferred here on Friday 7/18/14, is this stuff still going on??
jas permalinkSeptember 4, 2014 2:53 am
I can't believe i'm just now reading this. I have been wanting to speak with someone about this facility. They are very unorganized and their conditions are indeed inhumane. Nearly everyday, my boyfriend and brother complain about how it is so hot in there that the dry paint is beginning to melt off the walls. On "seg" they only receive showers on certain days and not everyday. They do not serve properly prepped food items. My boyfriend never eats breakfast, lunch, nor dinner because of this. This facility has numerous conditions that are unacceptable. They're too busy trying to take everything away from these guys instead of doing their job, providing the basic standards, and meeting the guidelines that they are suppose to meet.
Shayla permalinkNovember 8, 2014 12:33 am
I agree this is very inhumane. Who would allow something like this. People commit crimes but to treat them in this way in hopes of rehabilitation. Makes no sense at all!
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Traffic backed up for ten miles after a multiple car collision at SR 516 Tuesday afternoon.
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Description:... Sciences can provide custom microplate coating using advanced automated high throughput microplate processing equipment that dispenses and washes a variety of plate formats. We can also accommodate virtually any lot size of the following formats (with a volume range of 0.5-320 L): 8 and 12 well ...Microplate Coating Services from Affinity Life Sciences, Inc
Description:... Sciences can provide custom microplate coating using advanced automated high throughput microplate processing equipment that dispenses and washes a variety of plate formats. We can also accommodate virtually any lot size of the following formats (with a volume range of 0.5-320 L): 8 and 12 well ...CELL LAB QUANTA* Flow Cytometer from Beckman Coulter
Description:... and accurate cell counting. This flow cytometer has multiple excitation wavelengths, including UV, allowing flexible fluorochrome selection for a variety of multi-color applications typically only achievable with larger, more expensive systems. This multiparameter system is designed to meet the diverse ...ProteoExtract Protein Precipitation Kit from Calbiochem
Description:... one step. • The ProteoExtract™ Protein Precipitation Kit provides a fast and efficient method to concentrate and clean up proteins from a variety of sources. Detergents, chaotropes buffer reagents, salts and other interfering compounds stay in solution. Precipitated proteins can easily be ...SureLINK Bioconjugation Kit from KPL
Description:... for quantifying conjugation efficiencies. It enables conjugation of various tags including enzymes, fluorophores, biotin and other tags with a variety of proteins. The chemistry offered in the SureLINK Bioconjugation Kit is superior to conventional methods of bioconjugation such as ...NovaTaq DNA Polymerase from Novagen
Description:... suitable for a wide range of PCR applications. To ensure the highest purity and reproducible performance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase possesses an integral 5′→3′ exonuclease and lacks 3′→5′ ...Dyad Disciple Thermal Cycler Chassis from Bio-Rad
Description:... thermal cycler is a dual-bay expansion for the Dyad cycler. It may also be operated independently, using a PC. The Dyad Disciple accommodates a variety of interchangeable Alpha units (sample-holder, heat-pump assemblies), which hold different types vessels, as well as the Chromo4 real-time detector. ...HDA-GT12TM System from eGene, Inc.
Description:... detection design with solid-state light sources and micro-optical collectors makes the system highly reliable and affordable. Works across a wide variety of genetic applications including Microsatellites, AFLP, RFLP, RNA quality checks and oligonucleotide quality checks. Performs fast and ...General DNA Sequencing Services from Polymorphic DNA Technologies, Inc.
Description:... Polymorphic DNA Technologies also offers a DNA sequencing service. We can sequence a variety of templates including plasmids, PCR products, BACs, etc. Our DNA sequencing service starts with customer-provided templates such as purified ...


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The plan was to stay in Carrollton for five years, then move back to the family farm near Scottsbluff, Neb.
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