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Topic: FIR
Juhi Parmar Shroff makes a comeback with 'F.I.R'
Her comeback was long due, Juhi Parmar Shroff who after winning name and fame with… more
Small towns – The new hubs of serials
Small towns and rural audience is the new target segment for the serial makers. They… more
'FIR' will now deal with a new funny cop
Too much comedy, too much forgetfulness and too many errors will now rule "FIR", one… more
Aamir Ali & Sanjeeda Sheikh to wed on Friday
Mumbai, March 1 Popular television actors Aamir Ali and Sanjeeda Sheikh, who have been dating… more
Kavita Kaushik opens up about her best pal
Television actress Kavita Kaushik garnered fame with her character as Chandramukhi Chautala in TV serial… more
Kavita Kaushik not leaving 'FIR'
Talented actor Kavita Kaushik who plays the super hit role of inspector Chandramukhi Chautala in… more
Aamir Ali, Sanjeeda Sheikh planning to tie the knot in March?
The winners of "Nach Baliye Season 3″ – Aamir Ali and Sanjeeda Sheikh made it… more
Kavita Kaushik a.k.a inspector Chandramukhi Chautala to quit 'FIR'
Kavita Kaushik- the shining face of the TV serial "FIR" on SAB TV created quite… more


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I'm into many
lifestyles.I am alone. I do love kidsand often assist in
babysitting with a friend of mine. kids tend to like me more than
most adults.I was A carpenter by trade and enjoyed my
work.In my spare time I enjoy working with those who enjoy learning
play,discussing different practices and studies of the
supernatural,and making plans to help the communities where I
live.I love video games,movies,food,and hanging out with random
people. I'm in a medieval reenactment group.
I am stressed, bored, and changing
I am stressed, bored, and changing

subset: commoncrawl


text: 4 Great for the moneyPosted: 25/01/2010Bought this to control a Sony Bravia TV and Bluray, a Panasonic DVD/Surround system and a Samsung cable box.PLUS POINTS- TV and DVD codes worked straight away.-Online code upgrade process is really simple (required for the bluray player)-Very quick and simple setup (pre-installed codes and 'learn' function)BAD POINTS-Sharp corners (only minor, but its an irritation)-Centre buttons are too small.-Samsung cable box can only be partially controlled. It is not possible to scroll through menus or channels. This issue is waiting for resolution from oneforall.


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An awards reception will celebrate the 30 most notable works from practitioners and projects across the D.C. region.
Charlottesville architect M. Kirk Train was awarded the Society's highest honor—the William C. Noland Medal.
New research from the AIA, AIAF, and the ACSA advances design and public health's alignment.
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Design between architecture's practice and academy for areas in need.
In form and function, the higher-education campus is the United States' greatest contribution to architecture and urban planning.
2009 was a tough year for U.S. firms, but we found out who leads the pack in terms of profitability, design, and commitment to green.
Understanding how product certification contributes to greener buildings.


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That much is fairly obvious, but what isn't is the answer to the question of whether this band represents a new art form that should be highly praised for being ahead of its time, or if they're a bunch so cretinous one begins to lament the good oxygen being wasted by them. It's the kind of mystery that gives senators' wives something to do with their free time.
Ultimately, one must see Methadone Actors and decide for oneself. Do it while this is still a free country.


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Prep Tennis Roundup: Willmar topples Rocori
COLD SPRING -- The Willmar girl's tennis team won three singles matches and welcomed back 2-singles player Gabie Bierbaum as the Cardinals upended Rocori 5-2 Thursday in a Central Lakes Conference match.
Bierbaum missed Tuesday's win over Sartell because of soreness from an auto accident on Monday.
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Court of Queen's Bench
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22nd November 2007, 06:44 PM | #11 |
diyAudio Member
Join Date: Apr 2004
Location: MN
still haven't tried LTspice or Scilab but ended up writing a script to generate my own sine wave! I even wrote my own function to calculate sine based on taylor series. Its interesting how much precision (atleast 7 digits after the decimal point for 16 bit depth) is required to avoid rounding errors and take the precision upto or less than 1LSB.
I have yet to add the file creation part to write to a pcm wav file. Once that is done I will know how much distortion there is in the produced wave, or lack thereoff. In v2.0, complex waves - ability to add harmonics with variable amplitude! I wonder if these packages, or the languages they are written in, have the kind of precision for these type of operations. |
23rd November 2007, 09:02 AM | #12 | |
diyAudio Member
Join Date: Jul 2004
Location: Scottish Borders
If you do breakdown a squarewave into it's components one finds that the harmonics go out to infinity. That is the problem. The digital datastream runs out of bits in the range of 44Ks/S to 192ks/S. I have seen it estimated that a good approximation to a squarewave can be reconstructed if all the harmonics up to the tenth are included in the signal. To reconstruct a reasonable 96kHz squarewave would require a bit rate of 1Ms/S or looked at from CD datarates it should be possible to get a 4kHz squarewave from the DAC. I suspect that even a 4kHz sinewave coming out of a CD DAC will show significant distortion. Does anyone have any figures?
regards Andrew T. |
17th December 2007, 10:05 PM | #13 |
diyAudio Member
Join Date: Apr 2004
Location: MN
6 digits of precision(i.e. after the decimal point) and processing the sine series(taylor polynomial) up to the 9th degree is what you need in the least to create a sine wave with distortion buried in the noise floor.
Note that I did not say "without distortion". It is there, just not discernable. I had been fooling around with creating a wav file of a pure tone and observing what kind of loss of precision generates what kind of distortion and thought I'd share some results with you. It is a common belief that computing upto the 7th degree of the Taylor series for a sine function is a good enough approximation for a sine function. Wrong. You need alteast one more degree i.e. upto 9th degree to achieve a sine wave which has very little distortion. Here's a screen shot of the FFT of a 500hz sine wave produced with the sine function computing only upto the 7th degree - |
17th December 2007, 10:06 PM | #14 |
diyAudio Member
Join Date: Apr 2004
Location: MN
and here's a screen shot of the FFT of a 500hz sine wave produced with the same sine function, but computing upto the 9th degree -
FFTs were done with the spectrum analyzer provided in RMAA 6 with a Kaiser window of atleast 15. |
19th December 2007, 07:25 PM | #15 | |
diyAudio Member
Join Date: Nov 2002
Location: Netherlands
Re: Tone Generator - Multiple Tones and Variable Amplitude
Hi, FYI, have also a look here: http://www.fesb.hr/~mateljan/arta/ It works with 192 kHz soundcards as well. The build-in sine generator can do dual tones with adjustable ratio quite accurate. Attached a picture of a loop through measurement of my USB soundcard at 44.1 kHz. |
20th December 2007, 03:17 PM | #16 |
diyAudio Member
Join Date: Apr 2004
Location: MN
I don't think it can save it to a wav file, does it ?
I want a "generated" wav file, not a "recorded" wav file (to avoid introducing deficiences of the playback + recording chain). |
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"P.S., I love you, you, you, you," crooned the Mouser, mop-top-like, as he read the morose missive from AmeriTrade detailing how his personal info may have been compromised due to some unauthorized code in the online brokerages systems that allowed client information "to be retrieved by an external source." After recently receiving similar notes from other companies, the Kitty wondered if there was anyone on the planet who didnt have access to his personal info. "Its amusing that they all fall back to dependable old snail mail to break the bad news, too," laughed the Lynx. Alas, Le Chat, who was in San Francisco to attend the Intel Developer Forum, soon forgot his personal security woes and was thoroughly mesmerized by Gordon Moorethe Intel co-founder who proclaimed through his now-legendary Moores Law that the number of transistors on computer chips would double every two yearsas he told the crowd that the fundamental limitations of electronics would probably see his theory hit a wall in the next decade or so.
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As the Tabby tossed a tortilla toward his tonsils, a companion told him that AMD is rumored to be using components from faulty quad-core chips to create a triple-core offering. Triple-core chips could come in handy for graphics cards, and Big Blue reportedly already makes a similar three-headed beast thats used in the Xbox 360, claimed the pal. Another game geek accompanying El Gato said hed heard some folks at IDF talking about how Intel just acquired Havok, a game developer based in Ireland. "Well, Intel needs to keep up with AMD on all fronts, I guess," said Spence. As the Furry One ordered another round for the table, one of his compadres noted that Dell has created a supercomputer, called Legion, for University College in London. Legion, which Dell touts as a low-cost supercomputer, supposedly will pack the punch of more than 3,000 PCs. "Wow, instead of IBMs Blue Gene playing chess against Gary Kasparov, maybe the low-cost Legion can play Hacky Sack against Dell Dude Steven," cackled the Kitty.
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Kukla's Korner Hockey
The Canadian Ducks
by Paul on 11/20/06 at 09:59 AM ET
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With Brian Burke in charge, the Ducks are becoming as Canadian as curling, smoked meat and The Tragically Hip. They're even more Canadian than the Maple Leafs. Of the 22 players who have skated for the Ducks this season, four are Americans (Todd Marchant, Ryan Shannon, George Parros and Ian Moran) and two are Europeans (Teemu Selanne and Samuel Pahlsson). The other 16 are from north of the border. The only Russian is goaltender Ilya Bryzgalov, and there are no Czechs or Slovaks.read on
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Personnel of the De Beque Fire Protection District hope building a larger fire station will make their operations faster, safer and more efficient.
The district hopes to break ground as early as July on a 15,000-square-foot station at 4580 Interstate 70 Frontage Road, according to District Fire Chief Mike Harvey. The new station will house offices, a community training room, built-in training props, a kitchen and five drive-through bays for the district's 10 trucks.
A man was twice convicted of child sexual assault — and under court order to stay away from children under 18 — before he started having sex with a 14-year-old girl, as evidenced by the couple's 3-year-old daughter, according to an arrest warrant affidavit.
It isn't an attempt to wrestle control of public lands from the federal … more
Global instability — and specifically the current Russian-backed unrest in … more
March appears destined to come in like a lion in western Colorado, as another … more
Windows smashed with bat … more
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Our newest guidebook to Grand Valley cycling.
Enjoy this scenic photo gallery of the Colorado National Monument.
CMU and local high school basketball playoffs, wildlife struggles continue.
Action from all three days of Colorado High School State Wrestling.
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Yvette Castillon has done her homework to open a child care center in Clifton. … more
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text:
- Episode Description
A bored colonial on a Malaysian plantation employs an exotic accomplice--an earwig--in his gruesome plot to assassinate a romantic rival.
- Cast & Crew
Joanna Pettet
Jack Laird
John Williams (II)
Joseph Alves Jr.
Tom Helmore
- Fan Reviews (3)
the earwig
The Caterpillar
Extremely memorable
- Trivia & Quotes
Quotes (9)
Host: Good evening. I'm your little old curator in this museum which we call the Night Gallery. There are horror stories and horror stories. Elements of terror that take myriad forms. But this item has a built-in terror which can refrigerate even the most dispassionate amongst us. It has to do with a little beastie known as an earwig. A small bug that crawls into the human ear, and while inside, it doesn't whisper sweet nothings. It performs quite another function. Offered to you now on Night Gallery, a brand new nightmare which we call The Caterpillar.
Rhona: You are a moody one, Mr. Macy. You go from outrage to silence as if it were a quick tram ride.
Warwick: I see you've met our village entrepreneur. Macy: Robinson? Warwick: Yes, the only known rodent in captivity with a Christian name.
Macy: Bloody fool. I'm not soliciting an assassination. Tommy: Assassination? Oh, come now, young gentleman. Assassination? I shudder at the very word. The thought of a killing palpitates me, is what it does. Sends shivers up and down my body.
Tommy: An earwig. A kind of caterpillar. A thing almost as fine as a spider's web. It lives on wax, feeds on the innards of flowers, and it has a decided liking for the human ear. The natives hereabout, they have a distinct terror of it, they do. You see, it moves and it rests so lightly on a human being, that one is practically unconscious of it. Now, if you were to place one of these earwigs in a man's hair, just above his ear, well... once in the ear, it's a thousand-to-one chance of it ever coming out the same way again. You see, Mr. Macy, it can't turn round. Backing out is impossible. So it continues to feed as it goes, and it crawls right inside of the head.
Notes (1)
This episode is based on the short story "Boomerang" by Oscar Cook. This story was first published in the Christine Campbell Thomson anthology Switch on the Light (1931).
Trivia (1)
The Earwig Superstition: There is a long-standing European superstition that earwigs do, in fact, bore through the eardrum and tunnel through a person's brain, leaving eggs behind to hatch if they are female. Sources that cite this belief go back about a thousand years. This belief is also the origin of the insect's name. In May 2007, a 9-year-old boy in Albany, Oregon, US, had two spiders, one living and one dead, extracted from one of his ears. In 1862, British explorer John Speake, credited with being the European discoverer of the source of the Nile, suffered infections and other considerable ill effects from a beetle that crawled into his ear. In that case, however, the problems were not caused by the beetle itself, but by the attempts to remove it. Although there have been cases of maggots growing under human skin, usually introduced through a wound, medical literature has yet to record a case of burrowing through the eardrum.
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You might want to consider a Ford twin I-beam IFS. Here's the one under my '32 pickup which was done with air bags.
And the one under my '29 sedan delivery which was done with quarter elliptical leaf springs. I've never done one in a Bronco frame so I don't know what special problems you might encounter...but it might be worth a look as long as you are considering all options at this point. I don't know how helpful it might be, but you can click here for the step by step description, with pictures, of how the sedan delivery suspension was done. I prefer the beams from around '78 and '79 because they look like a traditional forged straight axle. But other years may be used as well.
Always learning...and sharing what I've learned. The Scratch-Built Hot Rod. |
Here is another twin I-beam build...which is under my roadster.
Always learning...and sharing what I've learned. The Scratch-Built Hot Rod. |
Always learning...and sharing what I've learned. The Scratch-Built Hot Rod. |
I have been following a build that really makes me sweat... Want to do something similar but would like to tub the rear, more of a pro street look.
I love this truck Without the top it doesn't look much like a truck at all... The fellow that owns this, I believe he should be driving it by now... |
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Has anyone ever cured themselves of binge eating?
(25 Posts)Just that.
I don't think that I ever will totally cure myself, but I do have a strong handle on it now, as I appear to be more able to identify when it might happen.
Anyone else, the best thing I have found so far is to chew 20 times. Anymore tips?
I've just read this book, which I have found to help me.
Can't get the link ishould?
Are you eating a completely balanced diet including plenty of fat? I had a problem when I was in University and in hindsight I think it's just that I wasn't eating properly (I was very active though, so stayed quite slim). Too many empty carbs (cereal, bread, cookies) and not enough protein and healthy fat. I just never really felt satisfied. Now I'm even more active but I eat mostly protein, vegetables and fruit, a little whole grain (oats, rice) and a lot of healthy fat (olive oil, avocado, nuts, dark chocolate etc.). Drinking lots of water is obviously helpful too and so is getting plenty of sleep and exercise (to regulate hormones and appetite). I read recently that wheat is an appetite stimulant and in my case that definitely seems to be true, so now I mostly avoid it.
The only thing that cured my tendency to binge eat was physically losing the weight. I was 210 lbs and did a bit of calorie control dieting to get down to the 160s. It was around that time that I realised I wasn't giving in to binge tendencies. I put it down to my stomach physically shrinking. Then I started 5:2 and am now 131 lbs and today even if I want to gorge on yummy food I can't cause I can't fit it inside my gut without being in physical pain.
Actually totally changing my diet really helped me. I cut out trigger foods and stuck to filling whole foods.
I will always have a tendency to emotionally eat but I certainly don't binge anymore like I did. I just think of the guilt I know I'll feel and it definitely makes me think twice.
Yes, have binged for years, got up to size. 20-22 and 18 stone . My solution might not help you though: I became a Christian and asked God to help be stop ruining myself .
Believe it or not I really feel differently about food now (pretty much an overnight transformation really in how I feel). I don't think about food much. Find it easy to make healthy choices, can have a small amount of pudding or whatever and not feel compelled to eat everything. Have also stopped thinking of 'being on the wagon' so I have nothing to fall off, iykwim? I've lost 3 stone in 3 months and feel like I haven't tried at all.
But I realise that this won't be the answer for everyone!
Hope this works!
I was wondering if you could tell me the main message in the book?
I used to binge eat from my mid teens then it turned into bulimia in my early 20's. It was only when I became pregnant at 24 that I stopped binge eating. My focus shifted from myself, and how I looked, to my baby, and all of a sudden food, control and image suddenly seemed less important. I realised that there were more important things in life and thankfully the urge has never returned. Horrible period of my life and I'm so glad I'm not a slave to food anymore. It dominated so much of my thoughts and time. Exhausting.
Yep that worked!
Inspiring blog from someone who cured themselves - hope this helps, OP
Thank you, it is all very helpful.
mameulah - its all about reprogramming your brain pathways in the end, but starts off talking about self esteem, and how most of us bingers lack self confidence. We then binge and hate ourselves even more.
The lady who wrote it is english, so its a fairly low key book, not 'american-ised' like a lot of them are.
Oh, and none of the advice she gives is about weight loss - its all about boosting self esteem (as above) and regaining a healthy lifestyle. Reaping the rewards for eating less and eating better food.
She talks about low GI or low GL food being far better for us, and ditching sugars and processed carbs.
Thank you ISHOULDCOCOA
Another recommendation for Brain Over Binge - both the book and the blog. It was the key element for me in stopping my bulimia.
mameulah whilst there is an element of biological urge to binge, typically driven by attempting to restrict and control food intake , and/or having what I believe is a particular sensitivity to 'trigger' foods (typically sugar and or starchy carbs), there is almost always an emotional element to bingeing.
Attempting to fight the urge to binge will never cure it. Bingeing serves a purpose for the person doing it - it is a coping mechanism or comfort (however bad it leaves them feeling). They may be suffering from repressed emotions, a lack of love and intimacy, work stress, problems in relationships (past or present), low self esteem, hatred of body...almost anything.
The poster above who found that religion solved her problem overnight is a great example, as is the one for whom having children was the change. They found a new and nourishing focus for their lives. It can be emotional or spiritual but it is an adjustment in these areas that will stop the bingeing. You must look at what your triggers are and where there are sources of stress or unhappiness, or something missing in your life. It will be different for everyone but address those things and the compulsion to binge will lessen, if not disappear. Not easy I know, but necessary..
Yep...oddly enough, when I stopped dieting! The binging stopped immediately, and I lost weight. Now the house is always filled to the brim with everything I love, and I can actually eat one biscuit and stop if that's what I feel like.
Yes, I had a few years of binge eating when I was in my late teens and totally obsessed with food, calories, my weight and dieting. It stopped when I went to University and got properly interested in other things. I never overeat now as I can't stand feeling full ... but I am very overweight. Its been a gradual creep upwards over 30+ years but I am too scared to go on any kind of conventional diet again, am frightened of going back to disordered eating, which is what I think dieting causes.
Mintyy I am starting work this year as an eating psychology coach, which of course incorporates nutrition, if you want to chat to me about possible changes to try in terms of what you eat I'd be happy to chat, I promise not to suggest any kind of 'diet'
Not yet despite or maybe because of successful membership of slimming world, reading beind chocolate which is specifically about this and trying to not think about dieting for months to take the pressure off.
My latest theory is to give up refined sugar completely as explained in sweet poison by David Gillespie. Don't know if will work yet but the idea is that most of us are addicted to sugar and that is what we are binging/grazing on. Cut the sugar and cut the drive to overeat. I doubt it will be easy but worth a try in my opinion.
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Below are the first 10 and last 10 pages of uncorrected machine-read text (when available) of this chapter, followed by the top 30 algorithmically extracted key phrases from the chapter as a whole.
Intended to provide our own search engines and external engines with highly rich, chapter-representative searchable text on the opening pages of each chapter.
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OCR for page 45
Ingestion of bacterially expressed clouble-stranclecl
RNA inhibits gene expression in planarians
Phillip A. Newmark*t, Peter W. Reddient, Francesc Cebria*, and Alejandro Sanchez Alvaradot
*Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, B107 Chemical Life Sciences Laboratory, 601 South Goodwin
Avenue, Urbana, IL 61801; and "Department of Neurobiology and Anatomy, University of Utah School of Medicine, 401 Medical Research and
Education Building, 20 North 1900 East, Salt Lake City, UT 84132
Freshwater planarian flatworms are capable of regenerating com-
plete organisms from tiny fragments of their boLlies; the basis for
this regenerative prowess is an experimentally accessible stem cell
population that is present in the adult planarian. The study of these
organisms, classic experimental models for investigating metazoan
regeneration, has been revitalized by the application of modern
molecular biological approaches. The identification of thousands
of unique planarian ESTs, coupled with large-scale whole-mount in
situ hybridization screens, and the ability to inhibit planarian gene
expression through double-stranded RNA-mediated genetic inter-
ference, provide a wealth of tools for studying the molecular
mechanisms that regulate tissue regeneration and stem cell biol-
ogy in these organisms. Here we show that, as in Caenorhabditis
elegant, ingestion of bacterially expressed double-stranded RNA
can inhibit gene expression in planarians. This inhibition persists
throughout the process of regeneration, allowing phenotypes
with disrupted regenerative patterning to be identified. These
results pave the way for large-scale screens for genes involved in
regenerative processes.
As young students, most of us learn about the amazing
abilities of certain animals (e.g., urodele amphibians) to
regenerate missing body parts. Despite the obvious biomedical
ramifications of understanding how such organisms are capable
of replacing lost parts, we remain largely in the dark about the
mechanisms involved (1~. One of the classic models for studying
animal regeneration, the freshwater planarian (2), has become
the focus of recent efforts to investigate the mechanisms under-
lying regenerative processes. The introduction of the powerful
techniques of molecular biology has reinvigorated interest in
these fascinating organisms and provided new tools for studying
this fundamental problem of developmental biology (3, 44.
X-irradiation and elegant transplantation experiments showed
that stem cells, referred to as neoblasts, serve as the source of
new tissue during planarian regeneration (2, 5~. These stem cells
can be specifically labeled and followed through the process of
regeneration and differentiation (64. To initiate the molecular
analysis of regeneration and stem cell regulation in planarians,
a collection of ~3,000 unique ESTs has been generated from a
clonal line of the planarian Schmidtea mediterranea (7~. Whole-
mount in situ hybridization techniques in planarians (8) have
been adapted to allow large-scale screens for gene expression
patterns in the whole organism, providing markers for nearly all
of the differentiated cell types (74. Most importantly, double-
stranded RNA (dsRNA)-mediated genetic interference (RNAi)
(9) can be used to inhibit specifically gene expression in planar-
ians (10), allowing gene function to be dissected in these animals
(11-13~. For example, RNAi knockdowns of the gene nou-
dc~rake, which encodes a fibroblast growth factor receptor-
related protein, result in ectopic brain formation throughout the
planarian (13), providing an initial glimpse into the mechanisms
www.pnas.org/cgi/cloi/ ~ 0. ~ 073/pnas. ~ 834205 ~ 00
underlying inhibitory signals first suggested by transplantation
experiments (24.
With the availability of thousands of planarian ESTs, and the
potential for using RNAi to identify genes involved in regener-
ative processes, we sought to develop a method for delivering
dsRNA that would facilitate large-scale screening efforts. Large-
scale RNAi-based screens in Cacuorhabditis elegans have been
expedited by a method devised by Timmons and Fire (14) for
using Escherichia cold to express and deliver dsRNA to the
nematode via feeding (154. This approach has been used to
screen for phenotypes resulting from the inhibition of all ORFs
encoded on C. elegans chromosome I (16) and for more recent
genomewide screens (17, 184. Here we show that ingestion of
bacteria that express dsRNA triggers the planarian RNAi re-
sponse, leading to specific gene inhibition. This inhibition is long
lasting, enabling gene expression to be inhibited throughout the
process of regeneration. After amputation of animals fed ap-
propriate targeting constructs, defects in the regenerative pro-
cess can be observed with specific molecular markers. These
results indicate that large-scale RNAi screens are possible in
planarians; such screens will facilitate a systematic dissection of
the mechanisms that regulate regeneration in these organisms.
Materials and Methods
Planarian Culture. A clonal, asexual strain (7) of S. mediterranea (19)
was used for the experiments and maintained as described (64.
Delivery of dsRbIA Via Bacterial Feeding. Restriction fragments
(~400-700 bp) from the targeted cDNAs (central marker B2,
GenBank accession no. AY068367; central marker B10, Gen-
Bank accession no. AY068369; gut marker D14, GenBank
accession no. AY066311; photoreceptor marker E30, GenBank
accession no. AY066982 j were ligated into the double T7 vector
pL4440 double T7 script II (15) and transfected into competent
DH1OB cells. A Gateway (Invitrogen)-compatible L4440 vector
was constructed by ligating Gateway Cassette C into SmaI-
digested L4440. Clone H.108.3a (GenBank accession no.
AY067513) was cloned into the resulting L4440 Gateway vector
by PCR amplification and cloning into pDONR221 (Invitrogen),
and subsequent transfer by using an LR cloning reaction into
L4440 Gateway. The L4440 unc-22 plasmid, pLT61, was pro-
vided by Andy Fire (Carnegie Institution of Washington, Balti-
more). The resulting recombinant plasmids were introduced into
competent (20) HT115(DE3) cells that lack RNase III and can
This paper results from the Arthur M. Sackler Colloquium of the National Acaclemy of
Sciences, "Regenerative Meclicine," held October 18-22, 2002, at the Arnold ancl Mabel
Beckman Center of the National Acaclemies of Science ancl Engineering in Irvine, CA.
Abbreviations: cisRNA, double-strancled RNA; RNAi, RNA-mediated genetic interference;
MP, metalloproteinase.
iTo whom corresponclence should be acidresseci. E-mail: firstname.lastname@example.com.
2003 by The National Acaclemy of Sciences of the USA
PNAS 1 September 30, 2003 1 vol. 100 1 suppl. 1 1 11861-11865
OCR for page 45
E cold
nl~narian food
/ pharynx
E. cold
liver, agarose, food coloring
Fig. 1. Method for feeding bacteria expressing dsRNA to planarians. dsRNA synthesis is induced in E. cold by using a vector developed for use in C. elegans (15)
that contains two T7 RNA polymerase promoters flanking the cDNA sequence of interest. After induction of dsRNA synthesis, bacteria are mixed with
homogenized liver (normal planarian food), ultra-low gelling temperature agarose, and red food coloring (for visualization of eating). This mixture solidifies,
and animals that ingest this artificial food become red and are exposed to the dsRNA.
be induced to express T7 polymerase in the presence of isopropyl
f-D-thiogalactoside (IPTG) (14, 154. For the experiments shown
in Fig. 2, 2-ml cultures in LB/carbenicillin were started with a
1:10 dilution of an overnight culture (grown in LB/carbenicillin
plus tetracycline) and incubated for 90 min at 37�C with shaking.
The cultures were induced with 0.4 mM IPTG and incubated for
an additional 4 h at 37�C. For the experiments using the H.108.3a
construct, 4-ml cultures were induced with 1 mM IPTG for 2 h
at 37�C. The cultures were then centrifuged and resuspended in
50-100 Al of an artificial food mix consisting of liver homoge-
nate, ultra-low gelling agarose, and food coloring (6~. Planarians
commenced eating almost immediately on exposure to the food
and finished eating within 15-30 min. Preliminary experiments
suggested that the most consistent inhibition was observed after
three feedings of the L4440 constructs. After the initial feeding,
the vast majority of planarians would not eat the artificial food
mixture on a daily feeding regimen. Thus, feedings were re-
peated every other day for a total of three times; in the case of
H.108.3a, feedings were repeated every third day. In situ hybrid-
ization was used as described below to monitor reduction in
transcript levels after ingestion of bacterially expressed dsRNA,
in both intact and regenerating animals.
Microinjection of dsRNA. dsRNA was prepared from H.108.3a by
using the MegaScript in vitro transcription system (Ambion,
Austin, TX). Intact planarians were injected three times with 32
nl dsRNA (or H2O for negative controls) by using a Drummond
Nanoject II microinjector (Broomall, PA) (104. Injections were
repeated for 3 consecutive days, and the worms were amputated,
allowed to regenerate, and then fixed and processed for immu-
nofluorescence as described below.
Whole-Mount in Situ Hybridization and Immunofluorescence. To
detect differences in transcript levels after dsRNA feeding,
intact and regenerating planarians were fixed and processed for
whole-mount in situ hybridization as described (7~. To monitor
photoreceptor axonal projections, planarians were fixed and
processed for whole-mount immunostaining as described (10~.
mAb VC-1 (21), a generous gift of Kiyokazu Agata (RIKEN,
Kobe, Japan), was used (1:5,000-1:10,000 dilution) to label the
photoreceptor axons; goat anti-mouse Alexa 488 (Molecular
Probes) was used at 1:400.
Bacterially Expressed dsRNA Inhibits Gene Expression in Planarians.
Genomewide RNAi-based screens have been conducted in C.
elegans by feeding nematodes E. cold that express dsRNA (17,
11862 1 www.pnas.org/cgi/doi/10.1073/pnas.1834205100
18). To test the feasibility of using this methodology to inhibit
gene expression in planarians, we designed constructs to target
several abundantly expressed, cell type-specific genes: two
centrally expressed metalloproteinases (MPs), B2 (a collage-
nase III-like MP) and B10 (an astacin-like MP); a digestive
system marker (D14.38~; and a photoreceptor-specific arrestin
(E30) (7~. Unlike C. elegans, planarians do not normally ingest
bacteria. Therefore, we resuspended dsRNA-expressing E. cold
cells in a planarian artificial food mixture containing homog-
enized liver, ultra-low gelling temperature agarose, and food
coloring (Fig. 14. This artificial food was then fed to the
planarians three times over the course of 5 days (see Materials
and Methods). Planarians that ingested the bacteria/food
mixture were viable, and we observed no perturbation of the
regenerative process after feeding of a control bacterial strain
containing the vector alone. However, when the bacteria
expressing specific dsRNAs were fed to the flatworms, we
observed inhibition of the targeted genes (Fig. 2~. The inhi-
bition was quite specific: targeting of one centrally expressed
MP did not affect transcript levels of the other centrally
expressed MP (Fig. 2a), nor were effects observed on gene
expression in other cell types, like the gut or photoreceptors.
Similar specificity was observed when gut-specific or photo-
receptor-specific genes were targeted (Fig. 2 b and c). Thus,
the observed inhibition is not caused by a general reduction in
transcript levels, but is specific to the targeted RNA.
Inhibition of gene expression could be observed as early as 1-2
days after the third feeding, and the effects were relatively long
lasting, with reduced levels still observed in intact animals 24
days postfeeding (data not shown). Complete regeneration takes
between 1 and 2 weeks in this species, depending on the site of
amputation. To test whether the inhibitory effect of the ingested
bacterially expressed dsRNA would perdure throughout the
regenerative process, we amputated planarians after their third
feeding, allowed them to regenerate, and then processed them
for whole-mount in situ hybridization. As illustrated in Fig. 2d,
inhibition of expression of the astacin-like MP B10 persists after
amputation and regeneration of new tissue. Similar results were
obtained after targeting the other centrally expressed MP (B2)
or genes expressed in other cell types (sensory neurons, gastro-
dermal cells), demonstrating the feasibility of using this method
to inhibit genes expressed in many cell types throughout the
process of regeneration.
RNAi by Feeding or Injection Can Produce Similar Phenotypes. Be-
cause RNAi has been applied only recently to studies of pla-
narian biology, only five RNAi-induced phenotypes have been
Newmark et a/.
OCR for page 45
Fig. 2. Inhibition of gene expression in planarians by ingestion of bacterially expressed dsRNA. (a) Specific inhibition of a centrally expressed MP. Both animals
were fed bacteria expressing a construct targeting an astacin-like MP (B10). One animal (Upper) was hybridized to detect the transcript encoded by another
centrally expressed MP (collagenase-like B2; positive signal in 10/10), whereas another animal (Lower) was hybridized to detect B10 (no detectable expression
in 32/36; significantly reduced expression in 3/36). When MP B2 was targeted, and animals were fixed and hybridized to detect B2 transcripts, no signal was
detected in 39/47 samples; 8/47 showed a strong reduction of transcript (data not shown). (b) Inhibition of photoreceptor expression of arrestin E30. (Upper)
The animals were fed bacteria targeting a gut-specific gene; photoreceptor expression of E30 is normal (7/7). (Lower) The animals were fed a construct targeting
photoreceptor-specific arrestin (reduction observed in 17/17). (c) Inhibition of expression in the gastrovascular system. Hybridization with gastrovascular-specific
probe D14.38. (Upper) The animal was fed a construct targeting a photo-receptor-specific gene (4/4 positive). (Lower) The animal was fed a construct targeting
D 14.38 (no signal i n 44/44 worms). (d) Inhibition persists throughout the regenerative process. (Upper) The anima Is were fed a control bacterial stra in, containing
the vector alone. (Lower) The animals were fed bacteria targeting astacin-like MP B 10. Three days after feed ing, planarians were cut into three parts and allowed
to regenerate for 9 days [12 days afterthe last feeding; animals are regenerated from head fragments (Left), trunk fragments (Center), and tail fragments (Right)].
The control worms reestablished expression of B10 (positive signal in 6/6 treated worms), whereas in the experimentalworms B10 expression was inhibited below
detectable levels (no detectable expression in 14/14 worms).
reported to date, and all of these have used microinjection to
deliver the dsRNA (10-134. To address the question of whether
ingestion of bacterially expressed dsRNA could also produce
phenotypes comparable to those observed after microinjection
of dsRNAs, we tested the efficacy of RNAi for inhibiting a
planarian gene (H.108.3a) (7) that is predicted to play a role in
axon guidance based on its similarity to the netrin receptor
encoded by frazzled (22~. Whole-mount in situ hybridization
analysis of numerous CNS-specific genes in the planarian Dug-
esia japonica showed that a planarian netrin gene is expressed in
the brain in the presumptive visual center to which the photo-
receptor axons project (23~. The planarian photoreceptors send
axonal projections across the midline to the visual center of the
brain; these cells can be visualized with mAb VC-1 (Fig. 3 a and
e) (21~. Given the role of netrin in axonal guidance in other
organisms (24-29), it is reasonable to predict that it plays a
similar role in guiding the photoreceptor axons to their proper
targets in the planarian brain.
Planarians injected with H.108.3a dsRNA were amputated
and allowed to regenerate; in all cases, these animals regen-
erated photoreceptor axons that did not target the brain
appropriately (Fig. 3 b-d). A range of phenotypes was ob-
served: in approximately one-third of the fragments, the
Newmark et a/.
photoreceptor axons appeared to approach or cross the mid-
line but did not extend posteriorly toward the visual center
(Fig. 3b); in another one-third, ectopic anterior projections
were observed, usually running along the midline (Fig. 3c J; in
the remaining samples, the axonal projections were highly
disorganized, with numerous ectopic projections (Fig. 3d).
These results clearly implicate H. 108.3a in photoreceptor
axonal targeting in planarians.
The dramatic phenotypes observed after injection of H.108.3a
dsRNA provide a useful measure by which to compare the
effects of dsRNA injections and feeding. Planarians were fed
bacterially expressed H.108.3a dsRNA; they were then ampu-
tated and allowed to regenerate. In two-thirds of the samples, the
regenerated photoreceptor axons were misrouted: the pheno-
typic classes produced after RNAi by feeding were strikingly
similar to those produced after RNAi by injection. Regenerated
photoreceptor neurons failed to send projections posteriorly
from the optic chiasmata (Fig. 3f), had abnormal optic chias-
mata (Fig. 3g), or had additional projections and patterning
defects (Fig. 3h). None of these phenotypes was observed in
animals fed bacteria expressing control dsRNA from the C.
elegans unc-22 gene, a negative control for bacteria expressing
dsRNA not expected to affect any S. mediterranea gene (Fig. 3e).
PNAS 1 September30, 2003 1 vol. 100 1 suppl. 1 1 11863
OCR for page 45
Thus, delivery of dsRNA by feeding is capable of generating
phenotypes comparable to those observed after microinjection
of dsRNA, albeit with lower penetrance.
Fig. 3. Introduction of H.108.3a dsRNA by injection (a-d) or feeding (e-h)
results in similar defects in axonal guidance after regeneration. (a) Control
injections; 16/18 regenerated animals display normal axonal trajectories; 2/18
show minor defects distinct from those shown below. (b-d) Phenotypes
observed after injection of H.108.3a dsRNA. (b) Regenerated photoreceptor
neurons failed to extend axons posteriorly from the optic chiasmata toward
the visual center of the brain (8/28 animals). (c) Regenerated photoreceptor
neurons had abnormal anterior projections from the optic chiasmata (1 1/28
animals). (d) Regenerated photoreceptors had ectopic projections or pattern-
ing defects (9/28 animals). (e) Control RNAi by feeding; animals were fed
bacteria expressing dsRNA from the C. elegans unc-22 gene. After amputation
and regeneration, 0/40 animals displayed any abnormalities in axon path-
finding. (f-h) Phenotypes observed after feeding of bacterially expressed
H.108.3a dsRNA. (fl Regenerated photoreceptor neurons failed to extend
axons posteriorly from the optic chiasmata (18/48 animals). (g) Regenerated
photoreceptors had abnormal anterior projections from the optic chiasmata
(10/48 animals). (h) Regenerated photoreceptors had ectopic projections or
patterning defects (4/48). The remainder (16/48) had no observable defects in
the pattern of axonal projections (data not shown). oc, optic chiasmata.
Arrowheads, posterior axon projections from optic chiasmata to brain. The
view is anteriortothetop in all frames. (Scale bars: a-d, 100,um; e-in, 125 ,um.)
1. Sanchez Alvarado, A. (2000) BioEssays 22, 578-590. ~ _
2. Br0ndsted, H. V. (1969) Planarian Regeneration (Pergamon, London). 5049-5054.
3. A~?~ta K ~ Watanahe K. (19991 .Semin. Cell Dev. 13~1. 10. 377-~3
~ 7 ~ ~ ~ � 7 �
Newmark, P. A. & Sanchez Alvarado, A. (2002) Nat. Rev. Genet. 3,
5. Baguna, J., Salo, E. & Auladell, C. (1989) Development (Cambridge, U.K) 107,
6. Newmark, P. & Sanchez Alvarado, A. (2000) Dev. Biol. 220, 142-153.
7. Sanchez Alvarado, A., Newmark, P. A., Robb, S. & Juste, R. (2002) Develop-
ment (Cambridge, U.K) 129, 5659-5665.
8. Umesono, Y., Watanabe, K. & Agata, K. (1997) Dev. Growth Differ. 39,
9. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E. & Mello, C. C. ~ ~ ^
(1998) Nature 391, 806-811.
11864 1 www.pnas.org/cgi/doi/10.1073/pnas.1834205100
The above results demonstrate that feeding bacterially expressed
dsRNA is an effective method for reducing gene expression in
planarians. This method is specific, affecting only the gene
targeted for disruption, and can be used to target genes ex-
pressed in a variety of cell types, including secretory cells, cells
of the gastrovascular system, and neurons. The sensitivity of
planarian neurons to microinjected dsRNA has already been
demonstrated for sensory neurons (10, 11) and cells of the CNS
(13~. This sensitivity to dsRNA is in marked contrast to C.
elegans, in which mature neurons are largely refractory to RNAi,
independent of whether the dsRNA is delivered via injection,
feeding, or soaking (15~.
The inhibitory effect of ingested dsRNA in planarians is relatively
long lasting, with inhibition observed over a period of several weeks
(up to at least 24 days; we have not yet tested later time points) after
feeding. This result is consistent with the 3-week inhibition reported
after dsRNA injection in the planarian Girardia tigrina (114. Crit-
ically, when planarians that have ingested bacterially expressed
dsRNA are amputated and allowed to regenerate, target gene
expression in numerous different cell types remains reduced
throughout the process of regeneration. As we show by targeting a
putative netrin receptor homologue, this inhibition can yield spe-
cific regeneration-defective phenotypes similar to those observed
after injection of dsRNA. The lower penetrance with which these
phenotypes are observed likely reflects the need for further opti-
mization of the feeding protocol.
The results reported here establish RNAi by bacterial feeding
as a method for inhibiting gene function in planarians. This
feeding methodology will allow much larger RNAi screens to be
conducted in planarians than would have been possible by using
the more technically demanding and time-consuming microin-
jection. When combined with molecular markers to visualize
specific cell types (7), such large-scale RNAi-based screens will
expedite discovery of genes required for the formation and
patterning of planarian cells, tissues, and organs and will facil-
itate dissection of the molecular basis of regeneration in these
fascinating organisms.
We thank Lisa Timmons and Andy Fire for sharing the bacterial strains
and plasmids used in this work, many helpful discussions, and once again
making it respectable to feed worms with RNA; Kiyokazu Agata for
providing the VC-1 mAb; Sumita Saha for help with the initial feeding
experiments; and our colleagues at the Carnegie Institution of Wash-
ington's Department of Embryology for their support. This work has
been supported by National Institutes of Health Grants RO-1 GM57260
(to A.S.A) and RO-1 HD043403 (to P.A.N.) and National Research Service
Award F32-GM19539 ito P.A.N.~. P.W.R. is a fellow of the Helen Hay
Whitney Foundation; F.C. is a Visiting Fulbright Scholar of the Generalitat
of Catalunya; and P.A.N. is a Damon Runyon Scholar supported by the
Damon Runyon Cancer Research Foundation (Grant DRS 33-03~.
lO Sanchez Alvarado. A. & Newmark, P. A. (19991 Proc. Natl. A cad. .\c'. U~?A 96.
11. Pineda, D., Gonzalez, J., Callaerts, P., Ikeo, K., Gehring, W. J. & Salo, E. (2000)
Proc. Natl. Acad. Sci. USA 97, 4525-4529.
12. Bueno, D., Fernandez-Rodriguez, J., Cardona, A., Hernandez-Hernandez, V.
& Romero, R. (2002) Dev. Biol. 252, 188-201.
13. Cebria, F., Kobayashi, C., Umesono, Y., Nakazawa, M., Mineta, K., Ikeo, K.,
Gojobori, T., Itoh, M., Taira, M., Sanchez Alvarado, A. & Agata, K. (2002)
Nature 419, 620-624.
14. Timmons, L. & Fire, A. (1998) Nature 395, 854.
15. Timmons, L., Court, D. L. & Fire, A. (2001) Ge~e 263, 103-112.
16. Fraser, A. G., Kamath, R. S., Zipperlen, P., Martinez-Campos, M., Sohrmann,
, . . . . .
M. & Ahringer, J. (2000) Nature 408, 325-330.
17. Kamath, R. S., Fraser, A. G., Dong, Y., Poulin, G., Durbin, R., Gotta, M.,
Newmark et al.
OCR for page 45
Kanapin, A., Le Bot, N., Moreno, S., Sohrmann, M., et al. (2003) Nature 421,
18. Ashrafi, K., Chang, F. Y., Watts, J. L., Fraser, A. G., Kamath, R. S., Ahringer,
J. & Ruvkun, G. (2003) Nature 421, 268-272.
19. Benazzi, M., 13allester, R., Bagur~a, J. & Puccinelli, I. (1972) Caryologia 25,
20. Chung, C. T., Niemela, S. L. & Miller, R. H. (1989) Proc. Natl. Acad. Sci. USA
86, 2172-2175.
21. Agata, K., Soejima, Y., Kato, K., Kobayashi, C., Umesono, Y. & Watanabe, K.
(1998) Zool. Sci. 15, 433-440.
22. Kolodziej, P. A., Timpe, L. C., Mitchell, K. J., Fried, S. R., Goodman, C. S., Jan,
L. Y. & Jan, Y. N. (1996) Cc~ll 87, 197-204.
Newmark et al.
23. Cebria, F., Nakazawa, M., Mineta, K., Ikeo, K., Gojobori, T. & Agata, K. (2002)
Dev. Growth Differ. 44, 135-146.
24. Kennedy, T. E., Serafini, T., de la Torre, J. R. & Tessier-Lavigne, M. (1994)
Cell 78, 425-435.
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27. Mitchell, K. J., Doyle, J. L., Serafini, T., Kennedy, T. E., Tessier-Lavigne, M.,
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29. Wadsworth, W. G. (2002) Trends Ne~,osci. 25, 423-429.
PNAS 1 September 30, 2003 1 vol. 100 1 suppl. 1 1 11865


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Blog Category: International
United Nations Volunteers: Recognizing the Role of Volunteers in Eradicating Poverty
Ban Ki-Moon, Secretary General of the United Nations, perfectly summed up the important role volunteers play in our world. Volunteering, he said, "is a source of community strength, resilience, solidarity and social cohesion. It brings positive social change by fostering respect for diversity, equality and the participation of all. It is among society's most vital assets."
United Way Celebrates Volunteers in India
Throughout the world, volunteers make a difference by showing up, helping out and inspiring others to get involved. Volunteer events celebrating the contributions of volunteers, such as India's second annual National Volunteering Week, held January 18-25, give United Ways and others a special opportunity to recognize the unique contributions volunteers give to their communities.
Perspectives on Volunteering and Philanthropy from the Middle East
The Emirates Foundation Youth Philanthropy Summit 2014 was held in Abu Dhabi, United Arab Emirates in early November and proved to be just as exciting and inspiring as the first summit held in 2013. As I wrote then and found to be true again this year, it is so wonderful to meet and learn from people from around the world who are committed to volunteer engagement.
International Volunteer Day: A Global Celebration of Volunteers
In recent months I've enjoyed sharing news about volunteering from around the world: Australia, Taiwan, and Romania. International Volunteer Day (IVF), celebrated each year on December 5, is the perfect day to shine a spotlight one more time on inspiring volunteer engagement in places outside the U.S.
Why World Toilet Day Matters
The world is a complicated place, and it takes comprehensive solutions to drive lasting change. We're seeing that kind of change improving lives in places like West Bengal, India, where something as simple as clean, working bathrooms in schools are empowering young women and opening more doors to economic opportunity.


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Angle Avenue Echouhada et rue des Temples Quartier Hivernage, Gueliz, Marrakech, Morocco (Show on map)
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Distance to airport:
Marrakech - Menara Airport (3.9 Km)
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Djemaa El-Fna (1.3 Km / 16 min walk)
Souk El Bahja Market (1.4 Km / 17 min walk)
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Universal Fit
Shure SE425 Earphone
"Shure has definitely outdone themselves with this wonderful product. Never have I experienced more clarity in a pair of IEMs. For someone who listens to alternative rock, pop, and an assorted array of indie music, I say that few IEMs can do better. The amount of detail displayed is extraordinary. Vocals are crystal clear and forward, especially female vocals. However, the bass is lacking a bit..." --Dinerenblanc
Shure SE535-CL Triple High-Definition MicroDriver Earphone with Detachable Cable (Clear)
" >>(first impressions) >>as tyl herstens said - "Ahhh! Someone slipped liquid gold into my ears! The genies at Shure did some real magic here." >>just wonderful ...handsome bass , rich and forward mids , in-offensive treble Impressions after 6..." --ayaflo
Shure Sound Isolating Earphone SE315 Hi-Definition Micro Speaker with Tuned Bass Port (Clear)
"This is a review for the Shure SE315's, first a little about myself I don't consider myself a hard core audiophile like most people on this site. I am 38 years old and I am just your everyday listener who appreciates quality sound. I have been into Hi-Fi audio since I was a teenager mostly full size speakers. What do I consider an audiophile, someone who pulls those ear buds out that..." --acain
Koss RUK 30K Noise Isolating In-Ear Stereophone, Black
"I tested it with Dr.Chesky's Binaural album and Depeche Mode flacs. What I like most is of course bass response..it is more than enough, maybe a bit exaggerated, aggressive. Overall, I am really satisfied this excessive response in some genres.The only real problem it is prone to loudness noise of the record.. Maybe this is because the drivers are too sensitive. I am not that high end..." --ajlan
Shure SE115 Sound Isolating Earphones
" Bought this last month, have been listening to it for the past 1month. This is a entry level IEM by Shure. For the price I paid I expected better sound but unfortunately I have heard better sounding IEMs going for a lower price. The cable is modular which is great. It makes easy change of cable should the original gets damage. Noise isolation is wonderful. I tried this ear-bud..." --slimfox
Shure Sound Isolating Earphone SE315 Hi-Definition Micro Speaker with Tuned Bass Port (Black)
"Just want to state up front my review rating is an absolute rating, not one based on comparative performance, pricing etc. Having had the Shure SE215s for a while and not enjoying them I thought I would go up the range and try the SE315s. At first I was impressed as the nasty spiky highs and wooly bass seemed to have gone but alas it was not to last. My overall thoughts..." --Krisman
Shure SCL5CL SHURE EARPHONES DUAL DRIVERS CLEAR E5
" Build Solid construction for earpieces. However after close to 2 years of usage the canal duct which the silicon sleeve sits on came loose from the main earpiece, not totally but loose enough to affect the quality of sound, especially bass. Wire was durable, however it oxidizes easily, and the oxide seeps into the main earpiece from the memory wire, which..." --broww
Shure PTH Push To Hear Control for Sound Isolating Earphones
" If you're tired of removing your IEMs to talk to someone, then this product is probably for you, despite its quirks. I wear my SE530s all day at the office and conduct most quick conversations through my PTH. The microphone is surprisingly good, even letting you conduct a conversation when it's pointed backwards. A few background noises like printers, scanners, or a fan pointed straight at it..." --maverickronin
NCAA Virginia Cavaliers SportBuds Headphones
SportBud headphones by Koss are molded in the colors of your favorite NCAA team, including their collegiate logo This 2 pair pack of ultra-lightweight in-ear SportBuds is perfect for music on the go You get both home and away colors This dual portable stereophone unit includes a see-through, compact, wind-up spool carrying case for easy storage and protection when not in use 4-foot straight...


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Posts tagged yellow dog linux
HELIOS PS3 Linux for fast and easy installation
HELIOS is offering PS3 owners a test server that runs on a simplified version of Yellow Dog Linux. The OS arrives via CD disk image and installs in about six minutes. Terra Soft Solutions, the brains behind Yellow Dog Linux, agreed to allow Helios to distribute this "lite" version, catering specif...
Yellow Dog Linux now supports wi-fi on the PS3
Woot! Finally! Terrasoft announced this week that they've released a new version of Yellow Dog Linux for the PS3, and this version includes support for wi-fi and fixes some USB printer issues. I'm pretty stoked about this; the lack of wi-fi support is why I wiped the Linux partition off my PS3. Now ...
Yellow Dog Linux -- free and loving it
That's right, boys and girls, now you can easily download, burn, rip, install and repeat (or something) your way to a free alternate OS on your PS3. Terra Soft has unleashed a flurry of mirrors for you to use in downloading Yellow Dog Linux for the PS3 -- use them! It's free, after all. You can even...
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Off the Grid: The Witcher Adventure Game and Star Wars Imperial Assault
Posted on Feb 3rd 2015 2:15PM


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This will be Breaking Bad's final year to win an Emmy Award, and to mark the occasion, Emmy nominee Aaron Paul is conducting a Breaking Bad scavenger hunt for those fans in the Hollywood area.
A little more than a week ahead of the 66th Primetime Emmy Awards are handed out, the Creative Arts Emmy Awards have taken place, offering justified praise to outstanding work (mainly) behind the scenes.
The odd thing is, it isnt even Saul Goodman that people are interested in calling here. As revealed a couple of weeks ago by series creators Vince Gilligan and Peter Gould, the southwestern criminal worlds go-to lawyer is going by the name Jimmy M. McGill when Better Call Saul begins its story.
A guy named Larry Shepherd wants to make a sequel to the award-winning series called Anastasia, with Val Kilmer and guitarist Slash as two cops who catch criminals in the days after Walter Whites stronghold of New Mexicos meth trade. Feel free to take a minute to catch your breath.
Nominations for the 66th Emmy Awards will be announced today! Last year's list of nominees included Game of Thrones, Breaking Bad, House of Cards, Girls and The Big Bang Theory, among others. Will those series make their way back onto the Outstanding Comedy and Drama list this time around?
Details about Better Call Saul have been kept under the rug and inside a desk drawer and behind a bookshelf, but Gould just confirmed that the series will definitely have a non-static timeline that will occur before, after and during the events of Breaking Bad. So does that mean well get to see Bryan Cranstons iconic Walter White again?
There's good news, bad news and a picture for Breaking Bad fans. The bad news is, the Breaking Bad prequel spinoff series Better Call Saul won't be premiering this fall as originally planned. Instead, AMC's pushing the premiere off to 2015.
MAJOR Breaking Bad spoilers beyond this point! Is it possible that Walter White may have lived to cook another day? Bryan Cranston seems willing to entertain the thought when he briefly discussed the ending of Breaking Bad in an interview with CNN's Ashleigh Banfield.
Epic Rap Battle put two AMC protagonists up against each other today, and in a battle of The Walking Dead's Rick Grimes and Breaking Bad's Walter White, I think the one who knocks may have come out on top. In all fairness though, Walter White had an extra season to develop his badassery, whereas Rick Grimes is still getting there.
It makes total sense that Better Call Saul is shaping up to be the must-see comedy of the year. And if it wasnt already, the recent addition of comedic maestro Michael McKean definitely puts it into the upper stratosphere.
If youve got something to sell, youd be hard pressed to find an easier way to promote it than getting a few famous faces together. And thats exactly what actor David Ury did in the amusing ad above for his upcoming book Everybody Dies: A Childrens Book for Grown-Ups!.
One thing is for certain: we're all very, very excited for AMCs upcoming new series, Better Call Saul. Since the day the spin-off was announced, fans of Breaking Bad have gone off the deep-end of excitement. We here at Cinemablend know that it sucks to be out of the loop, so we've compile everything we know about your new favorite TV series thus far. Don't say we never did anything nice for ya!
Marie Schrader will be taking her purple show on the road. The Breaking Bad actress known as Betsy Brandt has nabbed a recurring role on Showtime's Masters of Sex for its upcoming second season, recurring alongside the series stars Michael Sheen and Lizzy Caplan.
Its a tricky thing, bringing characters from a beloved series onto a different show within the same universe. But not once has that worry gone through our heads regarding Vince Gilligans Breaking Bad spin-off series, Better Call Saul. Especially with the news that Aaron Paul will be reprising his role as Jesse Pinkman. Now were just thinking about how we're going to lose our ever-loving minds in the best way.
One of the major up-sides to AMC's Better Call Saul series being a prequel is that it opens up the opportunity to bring back pretty much anyone from Breaking Bad at some point or another, whether it be for a cameo or a bigger arc in the series.


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Baked Potato, Leek and Apple Latkes
Make potato latkes healthier by adding vitamin- and fiber-rich apples to the potato cakes, spraying them with olive oil, and baking rather than deep-frying them. Kids can be in charge of peeling the potatoes and apples, squeezing the moisture out of them after they've been shredded and drained, and mixing all the ingredients together. They can also help in forming the cakes and spraying them with oil. Serve them topped with low-fat sour cream or applesauce.
Olive oil spray
1 large egg lightly beaten
1 large egg white, lightly beaten
2 large leeks
2 medium tart green apples, peeled and cored
4 medium russet potatoes, peeled
1 tablespoon olive oil
1/4 cup matzo meal
1 teaspoon salt
1/4 teaspoon freshly ground black pepper
Low-fat sour cream for serving
Preheat oven to 450 degrees.
Spray a large nonstick baking sheet with olive oil (you can hasten the total cooking time if your oven can accommodate two pans side by side).
Trim off the root end of the leeks and remove any dark or ragged outer leaves. Trim off the dark, coarse part, leaving only the white and light green portion of the leek. Split these light-colored portions in half lengthwise and thinly slice. Place sliced leeks into a bowl and cover with cold water. Slosh the leek slices around a bit to make sure the dirt is removed from between the leaves. Lift leek slices out of the water and into a colander to drain. If the leeks are very dirty, you may need to repeat this process.
Peel the potatoes, and peel and core the apples. Using the fine shredding blade of a food processor, shred the potatoes and apples then place them in a colander, sprinkle with a little salt, and set in the sink to drain for at least 20 minutes. Turn potatoes and apples out onto a double thickness of paper towels or a clean, dry dish towel and squeeze out excess moisture.
In a large bowl, combine whole eggs, egg whites, leeks, potatoes, and apples, and toss to combine. Mix in olive oil, matzo meal, salt, and pepper.
Using your hands, drop mixture onto prepared baking sheet(s) forming thin, flat patties about 3 inches across. Spray cakes lightly with olive oil and bake on the center rack of the oven until golden brown on the bottom, about 10 minutes. Turn cakes over and bake about 7 minutes longer, or until crisp and cooked through. Transfer cooked latkes to a platter and cook remaining batches in the same manner as the first. Before serving, reheat the previously cooked latkes in the hot oven for about 4 minutes.
Makes about 48 latkes.
Pear Fritters
In place of jelly doughnuts, try serving pear fritters for dessert. They may still be deep fried, but you'll sneak in an extra serving of nutritious whole fruit. Children can help with peeling the pears and sifting, mixing, and dusting the cooked fritters with powdered sugar. But because the oil is very hot, keep children away from the stove during cooking.
1 1/3 cups all-purpose flour
1 1/2 teaspoons double-acting baking powder
3 tablespoons granulated sugar
Pinch of salt
3 medium Bartlett pears, peeled, cored, and diced
1/2 cup milk
1 large egg, lightly beaten
Vegetable oil for deep-frying
Powdered sugar for garnish
In a large bowl, sift together flour, baking powder, granulated sugar, and salt. Add milk and egg and beat until batter is smooth. Stir in pear and mix until well combined.
Fill a large, heavy pot with vegetable oil to a depth of 1 1/2 inches and heat the oil until it registers 375 degrees Fahrenheit on a deep-fat thermometer. Carefully drop the batter by large spoonfuls into the hot oil in batches and fry for about 1 to 2 minutes per side until golden brown. Lift fritters from pan using tongs or a slotted spoon and transfer to paper towels to drain. Sprinkle with powdered sugar just before serving.
Makes about 24 fritters.
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Surge, 1996 - 2008
The Nature of Matter1996
Landmark, 1996-2012
Spinners, 1997
To the SurfaceSurveyed1997-2014
Silence of the Ground, 2000
Tracing Tides, 2001
Cultivated Objects,2003
Shoalwan:River Through Fire,River of Ice, 2003
Tidal Trace,2004 - 2013
Endless FormsMost Beautiful, 2006
Archipelago, 2008
Armillary, 2010
Dimensions: 32 x 80 x 80 in. Mixed media: hundreds of natural and some industrial materials, wood tables
In this piece my concentration is primarily on minutiae- that which is often overlooked or unnoticed - and so must be collected in greater numbers to have impact. This activity is what connects me to the natural world as it is an opportunity to observe at close hand the objects' formation, growing cycle, and eventual deterioration.
Museums, I suspect, keep their collections in boxes or drawers for study, categorization and labeling. the open boxes used in Accretion Tables reflect on museum practice but without the scientific analysis.
When collected and presented in an ordered way there is a feeling of wonder and curiosity but also a sense of repetition and timelessness. All the materials gave gone through their prime of life and now remain as relics of past glories. Repetition is found throughout the natural world and I continue to be fascinated with the slight variations we observe in close examination of these reoccurring forms. But rather than seeing it as duplication, for me, these nuances are reaffirming and can suggest a metaphor for the celebration of life.


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- Adult Adventure
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Cuban Five: Justice or Injustice?
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Grades: PK-5
390 students
Galaxy Elementary School serves 390 students in grades PK-5.
The student:teacher ratio of 13:1 is lower than the FL average of 15:1.
Minority enrollment is 95% of the student body (majority Black), which is more than the state average of 57%.
Galaxy Elementary School operates within the Palm Beach School District.
Online Public Schools in FL
The student population of 390 students has declined by 11% over five years.
The teacher population of 31 teachers has declined by 11% over five years.
Grades Offered | Grades PK-5 |
Total Students | 390 students |
Gender % | 48% Male / 52%Female |
Total Classroom Teachers | 31 teachers |
Students by Grade |
School Comparison
The student:teacher ratio of 13:1 has stayed the same over five years.
The school's diversity score of 0.27 is less than the state average of 0.45. The school's diversity has stayed relatively flat over five years.
District Comparison
The district's student population of 183,574 students has grown by 7% over five years.
School District Name | Palm Beach School District | |
Number of Schools Managed |
226 |
23 |
Number of Students Managed | 183,574 | 9,989 |
District Total Revenue | $1,945 MM | $138 MM |
District Spending | $2,123 MM | $159 MM |
District Revenue / Student | $11,390 | $13,793 |
District Spending / Student | $12,434 | $15,882 |
School Statewide Testing | View Education Department Test Scores |
Nearby Schools:
The nearest high school is Boynton Beach Community High School (1.4 miles away).
The nearest middle school is Congress Community Middle School (1.3 miles away)
The nearest elementary school is Daystar Academy Of Excellence Charter School (0.6 miles away)
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Boynton Beach Daystar Academy Of Excellence Charter School
Boynton Beach , FL 33435
(561)369-2323Charter School0.6 mi | 114 students | Gr. KG-5 -
Boynton Beach Poinciana Elementary School
1203 North Seacrest Blvd
Boynton Beach , FL 33435
(561)739-5700Magnet School0.7 mi | 529 students | Gr. KG-5 -
Boynton Beach Forest Park Elementary School
1201 Sw 3rd St
Boynton Beach , FL 33435
(561)292-6900Magnet School0.9 mi | 594 students | Gr. KG-5 -
Boynton Beach Virtual Community School
Boynton Beach , FL 33426
(561)434-8000 -
Boynton Beach Congress Community Middle School
101 South Congress Ave
Boynton Beach , FL 33426
(561)374-5600Magnet School1.3 mi | 1019 students | Gr. 6-8
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Recent Public School Articles
Updated February 13, 2015
Special education law is not easy to decipher, with several regulations that govern special education services for disabled students. In this article, learn about the core components of the laws, rights, and individual education plans that can help create the best public school environment for your child.
Many school districts have implemented technology initiatives that provide laptops or tablets to students. While these technologies open opportunities for learning outside of school, they also require parental oversight to ensure children aren't spending too much time online.
Turn your child into a stronger student by creating the right support structure and expectations. Use these nine tips to help maximize your child's academic success.


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£7,500 pa
Commercial Property to rent Bude Cycle Centre, Bude, Cornwall
Price on Application - Located towards the edge of this popular coastal town in a prime location which has seen successful development over recent years, an opportunity to lease this brand new first floor premises offering 90 sq metres of floor area. Extensive shared parking. More details › -
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£535 pcm
2 bedroom flat to rent 19 - 23 Queen Street, Bude, Cornwall, EX23
This spacious two double bedroom apartment is situated in the centre of Bude. With a spacious open plan living and kitchen area with vaulted ceilings and a large feature fireplace, two double bedrooms, bathroom with shower. The property benefits from gas central heating and double glazing through... More details › -
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£695 pcm
3 bedroom flat to rent Downs View, Bude, Cornwall, EX23
Completely refurbished throughout this spacious 2/3 bedroom split level apartment is situated in the popular Flexbury area over looking the golf course and within minutes of the town and beach. The property briefly comprises of an entrance staircase and landing, large lounge/dining room with sunn... More details › -
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1 bedroom flat to rent 4 Flexbury Avenue, Bude, Cornwall, EX23
Ground floor one bedroom flat situated in the popular Flexbury area of Bude close to the town centre and Crooklets beach. Lounge with open plan kitchen area inc oven & hob, double bedroom, shower room and communal gardens. Available unfurnished immediately. More details › -
£1,200 pcm
4 bedroom semi-detached house to rent Killerton Road, Bude, Cornwall
A superbly presented semi-detached family home with extensive gardens enjoying a most convenient position close to town centre and beaches. Comfortable and well appointed living space arranged over 3 storey's and offering 4 double bedrooms (3 en-suite). Entrance driveway, front garden and extens... More details › -
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£650 pcm
2 bedroom flat to rent Downs View, Bude, Cornwall
A luxury first floor 2 bedroom apartment in this sought after location being within walking distance of the local beaches and amenities. The apartment has the distinct advantage of gas fired central heating complemented by double glazed windows, and affords superb views over the golf course. Avai... More details › -
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£525 pcm
2 bedroom flat to rent 12 Burn View, Bude, Cornwall, EX23
This spacious two double bedroom first floor flat is situated in Bude's town centre within easy reach of the local amenities and beaches. The property has been redecorated throughout with some new carpets and comprises of an entrance hall, large open plan lounge/kitchen/diner with bay window and ... More details › -
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£575 pcm
3 bedroom flat to rent Princes Street, Bude, Cornwall, EX23
This spacious three bedroom split level flat is situated in the centre of Bude amongst all the local amenities and only minutes from the beach. The accommodation briefly comprises on the first floor, entrance hall, open plan living kitchen dining room inc double oven and hob and a bedroom. On the... More details › -
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£56,000 pa
Commercial property to rent Kings Hill Industrial Estate, Bude
DESCRIPTIONThe property comprises two large units, situated within a spacious yard space at the top of Kings Hill Industrial Estate. Both units are of a steel frame construction with pointed brick cladding to first floor level with profiled metal cladding covering the remaining More details ›Marketed by Kivells, Commercial. Call: 01566 487000 Local call rate


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PlantFiles: Common Fig, Edible Fig, Higo
Ficus carica 'Chicago Hardy'
Family: Moraceae (mor-AY-see-ee) (Info) Genus: Ficus (FY-kus) (Info) Species: carica (KAIR-ih-kuh) (Info) Cultivar: Chicago Hardy 7 vendors have this plant for sale. 18 members have or want this plant for trade. Category: Height: Spacing: Hardiness: Sun Exposure: Danger: Bloom Color: Bloom Time: Foliage: Other details: Soil pH requirements: Patent Information: Propagation Methods: Seed Collecting: | Click thumbnail to view: By amandaemily By pbyrley By mygardens By mygardens By DaylilySLP By DaylilySLP By DaylilySLP There are a total of 8 photos. |
3 positives
No neutrals
No negatives
Gardeners' Notes:
This plant has been said to grow in the following regions:
Bridgeport, Connecticut
Marathon, Florida
Washington, Illinois
Winthrop, Massachusetts
Aurora, Missouri
Omaha, Nebraska
Whiting, New Jersey
Brooklyn, New York
Croton On Hudson, New York
Efland, North Carolina
Wake Forest, North Carolina
Richfield, Ohio
Manassas, Virginia


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Book your room at Hyatt Place Flushing/LaGuardia Airport
Guest Room, 2 Queen Beds
Max Occupancy: 6 guests (up to 5 children)King Guest Room
Max Occupancy: 4 guests (up to 3 children)Flushing hotel with an indoor pool
Situated near the airport, in the city center, this hotel is close to Queens Botanical Garden, Langston Hughes Community Library and Cultural Center, and Citi Field. Also nearby are Flushing Meadows Park Pitch and Putt and Louis Armstrong Stadium.
Hotel Features
At Hyatt Place Flushing/LaGuardia Airport, recreational amenities include an indoor pool and a health club. The hotel also features a roundtrip airport shuttle and a bar/lounge.
Room Amenities
Flat-screen televisions come with premium cable channels. Guestrooms also feature complimentary wireless Internet access, complimentary high-speed (wired) Internet access, and sofa beds.
Information missing or incorrect? Tell us!Hotel Amenities
Hotel Amenities
Hyatt Place Flushing/LaGuardia Airport features an indoor pool and a health club. The hotel offers a coffee shop/café. A bar/lounge is on site where guests can unwind with a drink. Guests can enjoy a complimentary breakfast each morning. A computer station is located on site and high-speed wired and wireless Internet access is complimentary.
This 3-star property offers access to a 24-hour business center and a meeting room. Event facilities measuring 800 square feet (72 square meters) include conference space. This business-friendly hotel also offers a rooftop terrace, tour/ticket assistance, and multilingual staff. An area shuttle within 2 mi and a roundtrip airport shuttle (available on request) are complimentary for guests. Onsite parking is available (surcharge).
Hyatt Place Flushing/LaGuardia Airport is a smoke-free property.
- 24-hour front desk
- Free airport transportation
- Total number of rooms - 168
- Number of floors - 10
- Bar/lounge
- Coffee shop or café
- Free newspapers in lobby
- Free breakfast
- Conference space
- Dry cleaning/laundry service
- Elevator/lift
- Health club
- Tours/ticket assistance
- Free WiFi
- Free wired high-speed Internet
- Computer station
- One meeting room
- Multilingual staff
- Rooftop terrace
- Luggage storage
- Safe-deposit box at front desk
- Self parking (surcharge)
- Conference space size (meters) - 72
- Year Built 2014
- Free area shuttle
- 24-hour business center
- Conference space size (feet) - 800
- Smoke-free property
- Number of buildings/towers - 1
- Indoor pool
- Television in lobby
Available in all rooms: Free WiFi , Free wired high-speed Internet
Available in some public areas: Free WiFi , Free wired high-speed Internet
Self parking (surcharge)
Room Amenities
- Premium TV channels
- Pay movies
- Climate control
- Air conditioning
- Phone
- Refrigerator
- Coffee/tea maker
- Daily housekeeping
- Private bathroom
- Free toiletries
- Hair dryer
- Bathtub or shower
- Rainfall showerhead
- Iron/ironing board
- Desk
- Sofa bed
- Cribs/infant beds available
- Blackout drapes/curtains
- Cable TV service
- Flat-panel TV
- Free WiFi
- Free wired high-speed Internet
- Dial-up Internet access (surcharge)
- In-room safe (laptop compatible)
- Separate living room
- Down comforter
- Sofa bed size
- Connecting/adjoining rooms available
- Separate bedroom
Where to Eat
Guests are offered a complimentary hot/cold buffet breakfast each morning.
Gallery Menu Fresh - This lobby lounge specializes in American cuisine and serves lunch and dinner. A children's menu is available. Open 24 hours.
Nearby Things to Do
Recreational amenities at the hotel include an indoor pool and a health club.
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- Accessible bathroom
- Accessible path of travel
- Braille or raised signage
- Accessible parking
- In-room accessibility
- Roll-in shower
Hotel Policies
Check-in time starts at 3 PM
Minimum check-in age is 21
Special check-in instructions:
Check-out time is noon
Payment types
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- The name on the credit card used at check-in to pay for incidentals must be the primary name on the guestroom reservation.
Only service dogs are allowed
No rollaway/extra beds available
- Self parking fee: USD 20 per day (in/out privileges)
We should mention
This property offers transfers from the airport. Guests must contact the property with arrival details on arrival, using the contact information on the booking confirmation. Up to 2 children 17 years old and younger stay free when occupying the parent or guardian's room, using existing bedding. The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.