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Tumor expression of estrogen receptor (ER) is an important marker of prognosis, and is predictive of response to endocrine therapy in breast cancer. Several studies have observed that epigenetic events, such methylation of cytosines and deacetylation of histones, are involved in the complex mechanisms that regulate promoter transcription. However, the exact interplay of these factors in transcription activity is not well understood. In this study, we explored the relationship between ER expression status in tumor tissue samples and the methylation of the 5′ CpG promoter region of the estrogen receptor gene (ESR1) isolated from free circulating DNA (fcDNA) in plasma samples from breast cancer patients.
Patients (n = 110) with non-metastatic breast cancer had analyses performed of ER expression (luminal phenotype in tumor tissue, by immunohistochemistry method), and the ESR1-DNA methylation status (fcDNA in plasma, by quantitative methylation specific PCR technique).
Our results showed a significant association between presence of methylated ESR1 in patients with breast cancer and ER negative status in the tumor tissue (p = 0.0179). There was a trend towards a higher probability of ESR1-methylation in those phenotypes with poor prognosis i.e. 80% of triple negative patients, 60% of HER2 patients, compared to 28% and 5.9% of patients with better prognosis such as luminal A and luminal B, respectively.
Silencing, by methylation, of the promoter region of the ESR1 affects the expression of the estrogen receptor protein in tumors of breast cancer patients; high methylation of ESR1-DNA is associated with estrogen receptor negative status which, in turn, may be implicated in the patient's resistance to hormonal treatment in breast cancer. As such, epigenetic markers in plasma may be of interest as new targets for anticancer therapy, especially with respect to endocrine treatment.
Breast cancer; Methylation; Luminal phenotypesBackground
The therapeutic options indicated for patients with breast cancer continue to be based, principally, on clinical-pathology criteria. The incorporation of new immunohistochemistry and molecular biology markers into the diagnosis has advanced the knowledge of potential markers of prognosis and prediction of response to endocrine therapy in breast cancer. One of the biomarkers most used is the expression of estrogen and of progesterone receptors (ER and PR, respectively) [1]. However, only 2/3 of the patients diagnosed with breast cancer express ER at diagnosis (ER+), while the other 1/3 of the cases do not express the receptors (ER-), and which is associated with non-differentiated tumors, with high cell proliferation index, poor response to endocrine therapy and poor prognosis [2]. Some tumors which are ER + at the time of diagnosis become ER- in the course of the clinical evolution of the disease [3]. Also, 30-40% of the ER + patients will develop resistance to the anti-estrogen treatment and which will favor the appearance of distant metastases, and death.
To date, the molecular bases of the response to endocrine therapy are poorly understood. Recent studies have shown that heterogeneity of response and prediction of response to chemotherapy and sensitivity to hormone therapy is based on "molecular portraits" [4,5]. However, the list of genes implicated in prognosis may or may not necessarily relate to the clinical results obtained in response to treatment [6] and, as such, warrants further investigation.
To date, methylation of DNA is known as an epigenetic phenomenon which plays a decisive role in the regulation of signal translation processes. Under physiological conditions, this epigenetic event influences which genes are activated during the process of normal cell differentiation [7], the maintenance of "genetic imprinting" [8], inactivation of the X chromosome [9], genetic transcription repression [10] and the suppression of regions of parasite DNA [11]. However, these epigenetic events, when aberrant, have a determining role in the development of malignant tumor processes [12,13] and, as well, the suggestion is of an involvement in resistance to chemotherapy, radiotherapy and hormone therapy [14].
ER role is key since up to 1/3 of the patients who do not express ER (i.e. ER-), rarely respond to hormone treatment [15]. ER is coded-for by the ESR1 gene located at chromosome 6q25.1, the promoter region of which contains a linked CpG sequence in exon 1. In breast cancer cell lines such as MCF-7, T47-d and ZR75-1 [16] that express ER (i.e. ER+) this region is observed to be non-methylated, and is similar to that occurring in normal tissue. However, in cells lines from ER- breast cancer, such as MDA-MB-231, MDA-MB-435, MDA-MB-468, Hs578t and MCF-7/Adr, methylation is observed in >50% of cases [17]. Hence, determining the methylation status of the promoter region of the ERS1 could be critical since it represents one of the mechanisms by which the loss of ER expression is associated with breast cancer diagnosis. Blocking this process of methylation could be important since this could lead to patients who are resistant to hormone treatment becoming sensitive to hormone treatment [18].
Based on the literature, as well as on the experience in our own research group [19-21], we designed the present study to assess whether ER- expression in tumor tissue correlates with methylated status of the ESR1 in serum i.e. a mechanism of gene silencing that can explain, at least in part, the lack of hormone therapy efficacy in breast cancer. Also, we sought insight into fcDNA methylation and tumor phenotypes: Luminal A (LA), Luminal B (LB), Triple negative (TN) and Her2 +.
Study population
A total of 110 patients diagnosed as having non-metastatic breast cancer in the Hospital Universitario Virgen de las Nieves de Granada (Spain) were included in the study. Patient characteristics are summarized in Table 1. The study was approved by the Institutional Ethics Committee of the Hospital Universitario Virgen de las Nieves de Granada, and written informed consent was obtained from all study participants.
Table 1. Demographic characteristics of the breast cancer patients
Collection and processing of samples and DNA preparation
Blood samples (10 ml) were taken by venipuncture from all the study patients on introduction into the study and before the administration of any medication. The blood samples were collected into EDTA Vacutainer® tubes and coded before processing to ensure blinding with respect to sample provenance. The samples were transported at room temperature to the laboratory, centrifuged at 2000 g for 10 min at room temperature, the plasma obtained was distributed in 1 ml aliquots into 1 ml criotubes, and stored at -80°C until needed for processing.
- DNA isolation: DNA from plasma samples (2 ml per column) was obtained using QIAmp DNA Blood kit (QIAGEN Inc., CA) according to manufacturer's recommendations. A final elution volume was 200 μl and the extracted DNA was quantified spectrophotometrically. The amount of DNA recovered, measured as μg/sample, was 0.431 ± 0.019 (mean value ± standard error of the mean). The fcDNA samples were stored at −80°C until needed for analysis.
Quantitative Methylation Specific (QMS) polymerase chain reaction (PCR) analysis
- DNA bisulfite modification: Identical DNA sequences that differ only in methylation status can be amplified by means of Quantitative Methylation Specific PCR (QMS-PCR) [22]. Reagents required for the bisulfite modification of fcDNA were supplied in the CpGenome™ DNA Modification Kit (Intergen, MA). The process was performed according to manufacturer's recommendations. Sufficient fcDNA can be recovered to perform QMS-PCR from an amount of starting material as small as 0.001 μg. In brief, 100 μl of extracted fcDNA was treated with sodium bisulfite for 16 h, thereby converting all unmethylated cytosines to uracils, but leaving methylcytosines unaltered. After purification, the fcDNA obtained was dissolved in 20 μl of TE buffer and the modified DNA was spectrophotometrically quantified. Efficiency of fcDNA recovery after bisulfite modification was around 55% (data not shown). Recovered bisulfite-treated fcDNA (1 μl) was used in each well for SYBR green reaction. Modified DNA of standards and samples are stable for at least 2 months at −80°C. A sample of bisulfite-modified universally-methylated genomic DNA, (CpGenomeTM Universal Methylated DNA, Intergen, New York, NY) treated in the same way as patient samples and the concentration adjusted, after modification, to 2 μg/ml (quantified spectrophotometrically), served as internal standard in preparing serial dilutions (from 1 to 1/128 with MiliQ water) to construct a standard curve for Real-Time QMS-PCR. Each multi-well plate contained patient samples, serial dilutions of completely methylated DNA for constructing calibration curves, positive controls, and two wells with water used as negative controls ("blanks"). In all experiments, correlation coefficients for the calibration curves were >0.98, slopes ranged from 3.2 to 3.4, and PCR efficiencies were around 100%.
As found by other authors [19,20,23,24], some gene promoters were frequently observed to have methylated DNA in the plasma of cancer patients, albeit traces of methylated DNA may also be found in plasma of patients without cancer when highly sensitive quantitative techniques are used. Hence, cut-off points for the ESR1 methylated promoter was established from the receiver operating characteristics (ROC) curves i.e. selecting values that gave the maximal likelihood ratio (in current case the cut-of value was 0.02 relative units) [20]. Assuming levels of methylation of ESR1 < 0.02 relative units, "test of methylation (−)", was indicative of absence of the disease (physiological) while levels of methylation of ESR1 > 0.02 relative units measured in the plasma "test of methylation (+)" was indicative of presence of breast cancer (pathological level of methylation). Once the distribution of cases was established in the two groups as "test of methylation (−)" and "test of methylation (+)", the study proceeded to assess whether this characteristic was associated with the phenotype ER(+) and ER(−) in tumor tissue.
Using a method developed previously in our group [20,21], Quantitative Methylation Specific PCR (QMS-PCR) was performed with the iQ SYBR-Green Supermix Kit (BioRad Laboratories; Hercules, CA) according to the manufacturer's protocol. The sequences of the primers for ESR1 were selected from previous publications: ESR1 Genebank 2099 location to transcription start Promoter A [25]. The fluorescence value corresponding to each sample was converted into relative units of universally methylated DNA (umDNA; μg/mL) using the corresponding calibration curve adjusted by the software program of the QMS-PCR equipment. The PCR reaction was conducted in 96-well plates which contained: patient samples, successive dilutions for the calibration curve, 2 positive controls, and 2 negative controls or "blanks" (without DNA). In all cases the correlation coefficients for the calibration curves were ≥ 0.98, the linear slope was between 3.02 and 3.2, and the PCR efficacy was between 85 and 110%.
Immunohistochemical staining for ER, PR and HER2 expression in tumor tissue
Starting with surgically excised tissue preserved in formol, tumor pieces were embedded in paraffin and processed for staining with eosin-hematoxylin. ER and PR expression were evaluated in tumor tissue using the DAKO HORIZON automatic processor (Techmate Horizon). Monoclonal antibody kits were purchased from the manufacturer (DAKO M 7047 Clone 185 for ER and DAKO M 3569 Clone 636 for PR) and used according to the manufacturer's instructions. Nuclear staining indicates positive or negative. Positivity is expressed as intensity of staining and graded as weak (+) moderate (++) and strong (+++), and as percentage of cells stained. Subsequently, HER2 amplification in the tumor sample was with the DAKO K5206 kit. In those cases with HER2 (++), further analysis was with FISH using the DAKO K 5331, HER FISH PharmDxTM kit and hybridized in the DAKO HYBRIDIZER.
Statistical methods
The data obtained were analyzed using the following statistical tests: 1) description of the demographic and clinical-pathology variables using means, medians, percentiles, ranges and standard deviations; 2) relationships between ER expression in tumor and quantitative specimen level of ESR1 methylation using Chi-square (and Fisher's exact) test.
Results and discussion
The demographic and clinical-pathology characteristics of the participants at study entry are summarized in Tables 1 and 2. The cut-off points for ESR1 methylated promoter in plasma samples were established from the ROC curves, selecting values that gave the maximal likelihood ratio of 0.02 relative units for ESR1. From our results previously obtained [20], we assume that levels of methylation of ESR1 >0.02 relative units are indicative of the presence of disease (test "+" indicating breast cancer and pathologic level of methylation ESR1) whereas level of methylation <0.02 relative units indicating absence of disease (test "–" indicating no presence of breast cancer and physiologic level of methylation ESR1). Subsequently we sought correlations between methylation levels of the ESR1 promoter in the fcDNA samples and the absence of transcription and, in turn, with the lack of ER expression in tumor tissue.
Table 2. Clinico-pathological characteristics of the breast cancer patients
Using Chi-square (and Fisher's exact) test we studied the relationship between a positive test value for ESR1-DNA promoter methylation in plasma sample and ER- status in excised tumor samples from patients with breast cancer (p < 0.05). Similarly, we checked for correlations between negative test value for ESR1 vs. ER + in tumor tissue and observed that the relationship was statistically significant (p < 0.05). Results obtained showed an association between presence of methylated ESR1 in fcDNA from patients with breast cancer and ER- status in tumor, an observation that would be expected if promoter methylation leads to silencing of gene expression, and vice versa.
Similarly, we investigated the relationship between the gene expression silencing mechanism (using methylation of the ESR1 promoter i.e. epigenetic silencing) and the luminal phenotype of the tumor tissue; the hypothesis being that the predominant role of methylation is gene silencing (i.e. restricted expression) in the tumor. The Chi-square (and Fisher's exact) test showed a significantly (p < 0.05) higher percentage of ESR1 promoter methylation in those phenotypes with poorer prognosis i.e. 80% of the triple negative patients and 60% of patients with HER2 compared to 28% and 36% of patients with phenotypes of better prognosis such as luminal A and luminal B, respectively (Table 3, Figure 1).
Table 3. Percentage ER hypermethylation in relation to tumor phenotype
Figure 1. Histogram showing breast cancer subtype of poorer prognosis (TN and Her2) have higher percentage of ESR1-DNA promoter methylation > 0.02 relative units, while those phenotypes with better prognosis (luminal A and luminal B) the percentage of ESR1-DNA promoter methylation is < 0.02 relative units.
Based on these findings, we analyzed whether there could be subgroups of patients within each luminal phenotype such that a methylation profile could explain, at least in part, why some cases develop distant metastases despite having a good prognostic phenotype. We observed that, within each subgroup segregated with respect to ESR1 methylation in the fcDNA sample, there was a tendency towards a lower survival at 4.5 years of follow-up in each phenotype that had methylated ESR1 (ESR1 methylation level >0.02) compared to the subgroup in which the ESR1 methylation level was <0.02. However, these differences were not statistically significant, perhaps because of the small number of cases (Table 4).
Table 4. Differences in presentation of aberrant ER methylation within the luminal phenotype subgroups
It has been well documented that the ER expression in tumor tissue of breast cancer represents one of the principal prognostic factors of long-term survival, and is predictive of the disease response to hormone therapy. As such, the patients with tumors that are ER + are associated with a more favorable prognosis and the hormonal treatment of which is based on tamoxifen and/or aromatase inhibitors. However, in those patients that present tumors with ER-, the hormone treatment has little or no therapeutic value but there is an element of impact on the prognosis of the disease i.e. ER- is associated more frequently with those cases in which the course of the disease is more adverse. Around 25% of breast cancer patients do not express ER at the time of diagnosis and, as such, are resistant to hormonal therapy [2]. Also, in some cases the initial expression of ER + can change to ER- and negatively impact on the long-term course of the disease due to loss of sensitivity to the hormonal treatment [26]. Further research is warranted to explain this finding of change in phenotype [27].
In our literature search, we did find a few studies that correlated the epigenetic profile of methylation and its relationship with ER expression status but no study correlating the methylation with luminal phenotype. There have been earlier studies that investigated the role of methylation of various genes in search of independent markers of prognosis in breast cancer [28]. However, methylation of the promoter region of the ESR1 gene has received little attention in relation to the absence of ER expression in the corresponding tumor. Authors such as Widschwendter et al.[29] described a significant relationship (p = 0.015) between the APC methylation status and hormonal receptor status predictors in patients with breast cancer. Others studies, such as that by Yang et al.[30], suggested that, in ER + patients, there may be a higher frequency of hypermethylation of the promoter of the Twist gene, while methylation of the CDH1 gene occurs with higher frequency in patients the ER- tumors. Recent articles suggest that the hypermethylation of this gene promoter occurs predominantly in triple negative breast cancer [31]. This hypothesis is contested by other authors [32].
In the present study of cases in which we observed hypermethylation of the ESR1 promoter in plasma of the patients with breast cancer, we undertook an analysis of epigenetic signal silencing of the ESR1 gene in patients segregated with respect to luminal phenotypes, to the histopathology of the tumor, and to ER protein expression in the tumor. We evaluated ESR1-fcDNA methylation in peripheral plasma in relation to ER expression in tumor tissues. The results showed that the methylation of ERS1-fcDNA was correlated significantly with the absence of ER protein expression in the tumor, and vice versa (p = 0.018). This result is in accordance with that described by Lapidus et al. and Ottaviano et al.[18,33] in breast cancer cell lines. Essentially, these authors described that the promoter located in exon 1 of the ESR1 gene is observed to be highly methylated in the cell lines that do not express ER protein and, conversely, is not methylated in normal breast tissue as well as in the cell lines of breast cancer that express functioning estrogen receptors.
With respect to the luminal phenotype and ESR1 methylation status, we observed that, in those cases with the better prognosis phenotypes (luminal A and luminal B) the predominant result of the evaluation of ESR1-fcDNA methylation was negative i.e. there was a correlation between ER expression and better prognosis while, in the phenotype with poor prognosis (Her2-neu and triple negative), the evaluation was predominantly positive (p < 0.05). Further analysis of each luminal phenotype with respect to methylated ESR1 >0.02 relative units versus methylated ESR1 <0.02 relative units, showed that, within each subgroup, those that presented methylated ESR1 (and as such not expressing ER receptors) had a tendency towards shorter overall survival than those with a methylated ESR level < 0.02 relative units. Hence, it appears that the molecular variant of the methylated promoter region of the ESR1 gene carries prognostic information that is additional to the prognostic factors that are currently used in the breast cancer clinic.
From the above observations, the most relevant of the results obtained is that methylation, as an epigenetic event, can be one of the mechanisms implicated in the non-expression of ER in the tumor. We observed significant correlation between ESR1 DNA methylation in peripheral blood and silencing of ER expression in the tumor, and vice versa. This finding is of considerable interest especially since we observed, as well, the correlation between ESR1-fcDNA methylation and the ER expression in relation to tumor phenotype. The greater expression of the estrogen receptors in the phenotypes that are known to have a better prognosis (LA and LB) while showing significantly lower levels of ESR1 in peripheral blood. This contrasts with those phenotypes in which the ER expression is low or not present which, in turn is associated with poorer prognosis (triple negative and Her2). The mechanisms involved in the absence of ER expression are not known, and neither are the reasons for non-response to hormonal treatment. Of note, as well, is that tumors that express ER at the time of diagnosis can cease expressing ER over the time-course of the disease. Perhaps one of the causal events in the absence of ER expression (and its negative impact on the clinical evolution of the disease) is that tumors expressing ER at the time of diagnosis are methylated. In studies conducted to date, the known genetic alterations do not fully explain the processes but, based on the results from our study, perhaps methylation is the key to explaining, at least in part, some of the observations. Also, given that methylation is a reversible event, analyzing this reversibility can be useful in identifying the crucial point in the success, or otherwise, of hormonal treatment of breast cancer.
Our results indicate that the silencing of gene expression by methylation of the promoter region of the ESR1 gene of the estrogen receptor has an important role in the expression of the protein for which this gene codes (i.e. the estrogen receptor) in the primary tumor. The methylation of ESR1 in peripheral bold correlates significantly with the non-expression of ER in excised tumor tissue. As such, this measurement may add prognostic value in identifying luminal phenotypes with poor prognosis and, as well, those with potentially greater resistance to hormonal treatment. The protein is absent in ER- tumors when methylation occurs in the ESR1 promoter, and vice versa. These data demonstrate that analysis of methylation in the DNA from peripheral circulation (fcDNA) can help in determining prognosis or in predicting response to certain types of treatment. Of more importance, perhaps, is that since methylation is a reversible event, its modulation can constitute a future therapeutic target.
Reversal of methylation status and, as such, the genetic expression resulting from this mechanism, could cause a recovery of function of those genes responsible for the regulation of the normal cell cycle which, as a result of an alteration in the methylation pattern, are abnormally silenced. This could be of considerable interest because such an easily measured analyte (ESR1 DNA methylation in peripheral blood) can serve as biomarker, and probable therapeutic target against breast cancer.
ESR1: Estrogen receptor1 gene; 14-3-3-σ: Stratifin; ER: Estrogen receptor; fcDNA: Free circulating DNA; PR: Progesterone receptor; LA: Luminal A; LB: Luminal B; TN: Triple negative; QMS - PCR: Quantitative methylation-specific-polymerase chain reaction; ROC curve: Receiver operating characteristic curve.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
JM-G, SM, JRD and RdelM were significantly involved in patient recruitment and management, and placing the experimental findings into clinical perspective. BT-T, MIN, AC, CC, JL-P and SR conducted the in vitro analyses. JM-G and JM-A were involved in the interpretation and discussion of results; JM-G, JMRdeA and JRD conceived and designed the study, interpreted the data, and revised the manuscript. All the authors have read and approved the final manuscript.
We thank the nursing team of the Ginecology and Braquitherapy departments of the Hospital Universitario Virgen de las Nieves for logistics support in the management of the patients. Editorial assistance was by Dr. Peter R. Turner of Tscimed.com.
The study was funded, in part, by a grant from the Ministerio de Educación y Ciencia (CICYT: SAF 2004–00889). The funding body had no involvement in the generation of the results, or in their interpretation and the decision to publish.
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One of My Friend's Sister got a grand gift of god as Baby Girl and on same day after few hours My Friend's Jijaji given a grand party to all the relatives who are available their.
Jijaji says that below sentence is very true.
A woman can be mother, wife, aunt, grand-mother but she is daughter first. Daughter means happiness. Daughters are special. Without daughter world would be what garden without flower.


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Kukla's Korner Hockey
Kovalev Talks
by Paul on 02/07/06 at 01:40 PM ET
Comments (0)
Alexei Kovalev earned his pilot's licence in 1998, but the Togliatti native has also experienced the pain of having his Olympic gold medal hopes shot down twice since that year. Lucas Aykroyd caught up with Kovalev recently to discuss the 32-year-old Montreal Canadien's high-flying ways. IIHF.com: You surprised some people by deciding to sign a long-term deal with Montreal. Why did you make that decision? Alexei Kovalev: It was simple. They were the only team that was interested, and that's why I signed. I can't really shop around if nobody's giving me anything. It's not my decision.read on...discusses Russia's chances in the Olympics... update 12:12pm, Another Q & A from the IIHF, this time Andrew Podnieks, IIHF.com Olympic writer answers them-
How will USA coach Peter Laviolette take a series of negatives and turn them into positives? Coach Peter Laviolette has taken his Carolina Hurricanes to first place in the NHL, thanks in large part to his star players playing like star players but thanks also to his own successful methods. He'll need all his powers in Torino. The USA will be captained by a 44-year-old defenceman (Chris Chelios). The team lacks experience in goal and has too many young players to be a serious contender, but just maybe Laviolette will find a way to turn these negatives into positives and surprise the many critics who see his team not going very far in the tournament.more
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Poor girl have very painful experience with few monster black cocks!
If you like this video subscribe for new content and watch more similar videos in Hardcore category or try a little of this sex videos:
Crying won't help this teen!
But that wasn't all I had on mind when I lock the door...
Horse has got a very strong erection while watching a young couple fucking in the barn.
She'll forever be known as the girl that got turned into a cock sandwich, but the high fives these broskis and can throw at each other now are totally worth the degradation.
After a few bottles of beer she offered me something I couldn't refuse! She was a bit hairy, but virgin girl you just can't miss!
Yes, I was frightened and totally confused, but very fast I was amazed too.
Just relax little one, he won't eat you I promise.
Son's fiance was shocked when he unzipped his pants in all of his rage from getting fired from work.
This video should be banned from the internet.
There is one horny stallion and one hot girl unsatisfied...
Amateur country girl getting nailed while working on the field.
But she didn't know that everything what boy have, it belongs to daddy too.
I don't care what street cred you're trying to get in your hood - this is too much.


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Susie Davis-Miller
Researching on my Mother's side 'Wilson' has been rewarding & it could not have been completed without lots of good people & the information they have gathered. Still looking into my Father's side Daniel F. Davis son of Frank Davis out of Oneida/Scott cty, TN.
Also, working on input for my Virtual Cemeteries of all retirees/employees/AT&T Pioneers of America who worked for AT&T of Indiana.
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Tracy Wilson
Wilson family
Hi Susie,
Curious what your Wilson family line is. My grandfather on my father's side was Loren Wesley "Woody" Wilson. Woody was the brother of Joseph Wilson, Denny's father.
Tracy Wilson
Added by
Tracy Wilson
on Jan 28, 2015 4:28 AM
Ronald Wilson
Thanks for the photo!
Added by
on Sep 26, 2014 8:58 AM
RE: Davis Cem photo
Thanks for the photo. You can "cut & paste" the link below to see the Davis Cemetery page.
Added by
on Jul 02, 2012 7:05 AM
Davis Cem photo
I am working on updating the Genealogical Society of Marion County (GSMC)cemetery database for Warren twp, Marion County, IN. I'd like permission to post your photo of Davis Cemetery on the webpage for that cemetery. (It looks a lot worse now.) Thanks.
Added by
on Jul 01, 2012 4:43 PM
Rebecca Ann Boutwell(Becky)
Re ;ANDY Davis
If you check the link.I have David travis Davis linked to Thomas Jackson Davis. This was all I could find at the moment. I wanted to know if I could use your picture of Sally Hicks Davis. And your stone pictures for both her and Andy.
Elmer Davis was my GGrandfather.
Added by
Rebecca Ann Boutwell(Beck...
on May 04, 2011 9:31 AM
Rebecca Ann Boutwell(Becky)
Parents of Andy Davis
David Travis Davis b.4/8/1846 d.8/3/1917
Elizabeth 'Betsy' Ann Slaven Davis b 12/26/1847 d.5/3/1916.
Find A Grave# 52764492
Find A Grave# 22584835
Added by
Rebecca Ann Boutwell(Beck...
on May 02, 2011 7:35 AM
Nancy Heib
William "Indian Billie" Dragoo
You can see the memorial for William Dragoo (1770-1856), who is buried in the Pleasant Grove Cemetery, in Licking County Ohio, on this find-a-grave web site. His first wife was an Indian girl. The memorial for his second wife, Rebecca (Matheny) Dragoo-Price, of West Virginia, can also be found on find-a-grave.
Added by
Nancy Heib
on Sep 30, 2010 12:59 PM
Nancy Heib
Jacob Dragoo family
Thank you for the kind words. Do you have any "additional" information on the Dragoo family that you could share with me? (On your line perhaps). Thanks so much, Nancy
Added by
Nancy Heib
on Sep 29, 2010 11:20 AM
Andy Davis
Hi The Andy Davis I have Born 1870 died 1934 married to Sally Hicks had a son named Elmer Born 13 May 1903 died 27 Aug 1975.Elmer married my aunt Maude Coffey.Do you have Elmer?They are in the Hazel Valley Cemetery in Scott County.
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Jason Verrett
Injury Updates On Jason Verrett And Ryan Mathews
When the Chargers take on Miami (4-3) in South Florida Sunday afternoon, they hope to get back on the winning track, giving them momentum heading into contests against the Raiders, Rams and Ravens to finish out the month of November.
Chargers Rookie Corner Jason Verrett Out Indefinitely With Injured Shoulder
The San Diego Chargers will lose rookie cornerback Jason Verrett for at least one game and probably longer with a torn labrum in his left shoulder.
Team Grades: Chargers, Oliver Survive Upset Alert In Oakland
Okay, while they probably were not looking ahead, the Chargers sure were playing for their lives, escaping Oakland with a hard-fought 31-28 victory over their long-time rivals the Raiders.
Chargers Sign 1st-Round Pick Jason Verrett To 4-Year Deal
The San Diego Chargers have signed first-round draft pick Jason Verrett to a four-year contract.
Chargers Select TCU CB Jason Verrett With 25th Overall Pick In NFL Draft
The San Diego Chargers have taken TCU cornerback Jason Verrett with the 25th pick in the NFL draft.


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Six Nations 2013: Ireland 6-12 England
Ireland (3) 6
- Pens: O'Gara 2
England (6) 12
- Pens: Farrell 4
England's young team won a famous victory in testing conditions to end a 10-year Six Nations hoodoo in Dublin.
Four penalties from the boot of Owen Farrell saw them hold off a fierce Irish onslaught and move top of the championship table in what was the lowest-scoring match since the Five Nations became the Six Nations in 2000.
On a miserable, wet day it was seldom pretty, and when James Haskell was sin-binned with a quarter of the game left, Ronan O'Gara's second penalty saw Ireland level at 6-6.
The hosts looked set to take control, but with skipper Chris Robshaw leading from the front, the 14 men instead first stood firm and re-established their lead thanks to Farrell's accuracy.
Six Nations table
Played | Won | Points Difference | Points | |
England |
2 |
2 |
24 |
4 |
Scotland |
2 |
1 |
4 |
2 |
Wales |
2 |
1 |
2 |
2 |
Ireland |
2 |
1 |
2 |
2 |
Italy |
2 |
1 |
-19 |
2 |
France |
2 |
0 |
-15 |
0 |
While both sides barely looked like scoring a try - it was the first try-less game between the two sides since 1984 - Ireland made far more mistakes with ball in hand and seldom showed the fluency that saw them put Wales to the sword a week ago.
It means Stuart Lancaster's inexperienced England side are the only team in with a shout of the Grand Slam this year following their defeat of Scotland.
Farrell's penalty on three minutes gave England the lead, with handling errors costing both sides promising positions.
Ireland lost sparkling winger Simon Zebo to injury but saw his replacement, Keith Earls, make inroads with a scything run before each team reverted to kicking for territory.
The latent passions boiled over when Cian Healy stamped on Dan Cole's ankle at a ruck, triggering a melee of grabbing and shoving, while Farrell was lucky not to be sin-binned for dragging back his man without the ball.
It took a typically sweet pass from Brian O'Driscoll to set Earls clear again, only for another knock-on - this time from Mike McCarthy - to toss away the momentum.
Farrell made it 6-0 with unerring accuracy just before the half-hour, and Ireland's problems mounted when playmaker Jonny Sexton limped off after appearing to injure his hamstring.
The home side's travails were summed up by skipper Jamie Heaslip twice spilling regulation high balls, but within four minutes of the restart Ireland had a lifeline. Referee Jerome Garces penalised England for collapsing the scrum and O'Gara stroked over his kick.
Official match stats
Ireland | England | |
58% |
Possession |
42% |
57% |
Territory |
43% |
4 (0) |
Scrums won (lost) |
7 (3) |
15 (3) |
Line-outs won (lost) |
9 (4) |
11 |
Pens conceded |
14 |
73 |
Rucks won |
45 |
42 |
Possession kicked |
44 |
68 (3) |
Tackles made (missed) |
101 (11) |
1 |
Offloads |
1 |
3 |
Line breaks |
0 |
87 |
Ball carries |
55 |
211 |
Metres made |
165 |
(provided by accenture)
For the first time the men in white looked rattled. Tom Youngs's line-out throw twice went wayward; with Lancaster responding by sending on Manu Tuilagi and Courtney Lawes for Billy Twelvetrees and Joe Launchbury, and then Dylan Hartley for Tom Youngs.
As the rain continued to sweep in, the game appeared to turn just before the hour.
Haskell was sin-binned for interfering on the wrong side of a ruck, O'Gara drilled over the penalty, and the sides were level.
Yellow cards had hurt England in their last two Six Nations clashes in the city but this young side had other ideas.
A cute kick through from Farrell sent Rob Kearney scampering back and, from the resulting line-out, Ben Youngs almost put Tuilagi under the posts with a chip, only for the ball to bounce away from the centre's grasp.
To the crowd's dismay, referee Garces had already spotted Irish hands in the ruck and Farrell stroked over his third successful penalty. He then made it 12-6 when Kearney was pinged for holding on.
With the minutes ticking away and nerves tightening, O'Gara had a chance to cut the lead but pushed his penalty wide, while Alex Goode was impeccable under Ireland's high kicks.
Farrell narrowly missed from wide on the right after O'Gara was again pressurised into an error but Ireland had nothing left, and England were able to run the clock down to secure their first Championship win in Dublin since 2003.
Team line-ups
Ireland: Kearney; Gilroy, O'Driscoll, D'Arcy, Zebo; Sexton, Murray; Healy, Best, Ross, McCarthy, Ryan, O'Mahony, O'Brien, Heaslip.
Replacements: Earls for Zebo (10), O'Gara for Sexton (31), S Cronin for Best (74), Kilcoyne for Healy (74), Fitzpatrick for Ross (78), Henry for O'Brien (66), O'Callaghan for Ryan (65).
Not Used: Reddan.
England: Goode; Ashton, Barritt, Twelvetrees, Brown; Farrell, B Youngs, Marler, T Youngs, Cole, Launchbury, Parling, Haskell, Robshaw, Wood, Lawes.
Replacements: Tuilagi for Twelvetrees (47), Hartley for T. Youngs (50), Wilson for Cole (76), Lawes for Launchbury (47), Waldrom for Lawes (71).
Not Used: Vunipola, Care, Flood.
Sin Bin: Haskell (56).
Att: 51,000
Ref: Jerome Garces (France).


text:
Open iTunes to preview, buy, and download this movie.
Plot Summary
Avery had it all: his beautiful girlfriend, Krista, a young son and a full scholarship ahead of him. But in the blink of an eye, it was all taken away. What started as a night out with his buddies ended with the shooting of a cop and Avery behind bars. He's innocent, but that doesn't matter in a place that has its own set of rules. As Krista works to prove his innocence, Avery struggles to survive the day-to-day hardships of prison life. In the tradition of "Oz" comes a story of what it takes to get by in the toughest place on earth, starring Richard T. Jones (The Wood), Gabriel Casseus (Black Hawk Down), De'Aundre Bonds (3 Strikes), Melissa De Sousa (The Best Man), Bill Nunn (Spider-Man) and rap superstar Master P.
Rotten Tomatoes Movie Reviews
58%- Reviews Counted: 12
- Fresh: 7
- Rotten: 5
- Average Rating: 5.8/10
Top Critics' Reviews
Fresh: What makes the movie memorable is its authenticity.
Rotten: Doesn't tell you much about prison life that the movies ... haven't told you already.
Fresh: Despite a low budget and predictable story line, Lockdown has undeniable power to it, fired by some fine performances and a terrifying portrayal of prison life that rings disturbingly true.
Customer Reviews
Very Good Movie
Good movie for a day off in the House
Theee movieee
This is a great movie with a great message.
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text:
EUGENE – GoDucks.com editor Rob Moseley sits down with Mike Skow to recap the Oregon Ducks' historic 2014 season and take a glimpse into what 2015 has in store.
Web Extra: Ducks, FSU press conference
LOS ANGELES – Several Oregon players & defensive coordinator Don Pellum, along with Florida State quarterback Jameis Winston, teammates and the Seminoles' co-offensive coordinators talk to the media on Sunday leading up to the 2015 Rose Bowl. Oregon defensive coordinator Don Pellum: Oregon linebacker Tony Washington: Oregon defensive back Troy Hill: Oregon defensive lineman DeForest … Continue reading »
Web Extra: Ducks at Disneyland!
ANAHEIM, California – The Oregon Ducks football team is in Southern California and preparing for the Rose Bowl. Here's what they had to say after the first gathering of the two teams at Disneyland and California Adventure on Saturday. Oregon head coach Mark Helfrich: Oregon quarterback Marcus Mariota: Oregon center Hroniss Grasu: Oregon linebacker Tony … Continue reading »
Web Extra: Ducks Before Heading to L.A.
Oregon quarterback Marcus Mariota: Oregon center Hroniss Grasu: Oregon wide receiver Byron Marshall: Oregon offensive lineman Hamani Stevens: Oregon offensive lineman Andre Yruretegoyena: Oregon wide receiver Bralon Addison: Oregon safety Erick Dargan: Oregon linebacker Tony Washington: Oregon safety Reggie Daniels:
Web Extra: Ducks talk Rose Bowl, FSU
EUGENE, Oregon – Oregon football players and head coach Mark Helfrich talk about the Rose Bowl and their college football semifinal playoff game against Florida State on Tuesday. Oregon head coach Mark Helfrich: Oregon running back Royce Freeman: Oregon offensive lineman Hamani Stevens: Oregon defensive lineman Arik Armstead: Oregon linebacker Derrick Malone: Oregon defensive back … Continue reading »


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CK Catering - Creative gourmet menus, professional friendly staff, elegant yet fun service.
From the first passed hors d'ouevres to the late night snack let CK Catering create the perfect menu for your event.
Country Kitchen Catering, LLC - Providing the highest quality Buffet luncheons, dinners, pig roasts, picnics, bbq's and more to Michigan's great southwest for over 40 years.
We will also be having a a Tasting Saturday April 18th @ The Morris Supper Club in Niles MI.Registaration will begin March 9th.
Menus (Lakeland Meadowbrook Only)


text:
Product Review: Stevia
Update: I have switched brands and now use NuNaturals stevia.
There are times when I want something sweet and don't want to increase my blood sugar level. This is when I reach for stevia, an herb 300 times as sweet as sugar with a glycemic index (GI) of 0.
This low glycemic, low carbohydrate sweetener is widely used in Japan. In the U.S., the sugar lobby has long fought to ban this miracle sweetener; it is currently classified as a dietary supplement, though not as a food additive. Take note, that in Japan, stevia accounts for 40% of the sweetener market and is used in everything from colas to candy.
Stevia comes in various forms. You can buy it as a tincture or powder. The only drawback to stevia? It sometimes has a slightly bitter, licoricey aftertaste.
I tested at least half a dozen stevia products and found NuNaturals stevia to have the best flavor and to be reasonably cost-effective. Purchase Nunaturals stevia here.
During these chilly days and nights, I enjoy a few drops of stevia in a piping hot cup of tea!
Here are all of my recipes that use stevia as a natural sweetener. Enjoy!
I have been using astraya stevia for years … unable to locate the original product … biogro.us now has theirs in another package that I question is the same product.. and selina celtic salt people has the same bottle but the stevia is not the same in that it hardly dissolves at all especially in hot drinks… strange .. any ideas and I couldn't see where to purchase from you on your website… thanks.
When I was living in CA, I found pure Stevia powder (no additives like in the little stevia sachet or paper bags like sweet'n low) manufactured or distributed by KAL.
I no longer live in the U.S but I have written them a while ago and I got a reply, you may check with them whether they still distribute it.
I can not get any pure Stevia powder where I live, but liquid stevia which is just water mixed with the Stevia powder and it is sold for very high prices while the powder could last for a long time and I could make my own liquid stevia to carry in my bag wheneven I wished.
The government wants us to use sugar and chemical sweetners for our own protection rather than a safe natural healthy alternative.
I use Stevia Extract in the Raw and LOVE it! I highly recommend this product for sweetening your drinks as well as baking. Check out their site – they have some recipes on there too! http://www.steviaextractintheraw.com
Do you have dairy free, no fruit, no gluten or yeast recipes with stevia as only sweetener?
Yes! I know a great gluten free, yeast free, & sugar free bread recipe! I got it from the 'paleo' diet, at marksdailyapple.com!
1 cup coconut flour
6 eggs(they make up for the texture of gluten!)
1/3 cup coconut oil
1/2 cup coconut milk
pinch sea salt
2 packets of stevia (or 2 tbsp of agave nectar!)
2 tsp baking soda
(mix all wet and dry ingredients separately first!)bake at 350 for 30 mins!
Yum!!! :)-
This bread will only rise a 'little' with the help of the baking soda!!!'
So it has a rich texture almost like a 'coffee-cake'. Oh! And please also add to the recipe ***'2 tsp of lemon juice'***, as it will activate the baking soda! :) -
thanks for this loaf receipte rene, just a quick question..i have candida too so i avoid all flours including the gluten free ones…but this coconut flour is sooooooooo hard to find in stores!!! is coconut powder the equivalent? because that i can find easy enough…
i've been on an anti-candida diet the past two months, and in so doing have discovered stevia, xylitol and yacon syrup (agave is prohibited on the diet as it is still processed by the body as a sugar). i never imagined i could have my cake and eat it too – my voracious sweet tooth is satisfied, and my blood sugar is stable, no more afternoon lulls. i adore your site and would love to see more recipes using these sweeteners!
I am so excited about this, I have Candida and so does my sister, my grandma got rid of hers through diet, but her sister died from it… and I cannot have gluten, sugar, fruit, dairy, or yeast of any kind and I just want to shout from the rooftop. Thank you!
I would love to see recipes using stevia as the sweetener as I also have diabetes. You are terrific, as are your recipes, both on line and in your book. Thank you!
The problem with Stevia is that in laboratory mice tests it caused low sperm count, testicular problems that also contribute to reproductive problems, and possible cancer. BUT A) This has not been proven in humans B) If humans have similar side effects, you would have to take a lot of Stevia on a regular basis (I'd imagine that refined Stevia products carry a higher risk if any) C) If you grow the plant your self (which also limits your intake of it) then it would be healthier for you. I grow my own Stevia, read about it http://gardenoftomorrow.com/stevia/stevia-the-all-natural-sugar-free-sweetener-234/
Growing one's own stevia is fun and simple – I have a pot of it sitting outside that's been doing very well. Fresh stevia has a very subtle taste. However, I also use green powdered stevia from the bulk section from the co-op. It's great in all forms.
I wish my partner would use stevia, too, but he loves his cola with high-fructose corn syrup. But he is his own person, and makes his own choices, as well all do. He has a vasectomy and we will never reproduce, so a "low sperm count" is no issue here. ;)
Could you please indicate how many teaspoons or tablespoons of liquid stevia are in a dropper-ful? Thank you.
When I was in Israel, I stayed with a family that had a plant in their backyard. I noticed they pulled leaves from the plant and put it into their tea. It was a stevia plant! Can't get any more natural than that!!!


text:
From Comcast SportsNetENGLEWOOD, Colo. (AP) -- Veteran safety Brian Dawkins is retiring from the NFL after 16 seasons.Dawkins posted the news on his Twitter account Monday, thanking the teams he played for, his coaches and teammates and fans. Dawkins spent his first 13 seasons with the Philadelphia Eagles and his last three with the Denver Broncos.He suffered a serious neck injury in December that sidelined him for the stretch run and the Broncos' two playoff games."I would like to thank the Eagles & the Broncos 4 believing in me," he tweeted. "I would like 2 thank all my teammates & coaches that I have been blessed 2 go to battle with. Along with u, the fans 4 helping make my career 1 that I have enjoyed tremendously. In other words. I am announcing my retirement from the NFL."Broncos boss John Elway replied via Twitter: "Congratulations on a Hall of Fame career, Dawk!!!"Nobody played safety longer in the NFL than Dawkins, 38. The only other safeties to log 16 seasons in the pros were Hall of Famer Paul Krause and Eugene Robinson.Longevity isn't normally associated with the position where the hardest hits are both received and delivered."If you look at the majority of the huge collisions, they come in that second level back there," Dawkins said last season. "Safeties are 15-20 yards deep sometimes, we're coming down running full speed at somebody that's running full speed at us a lot of times, so those collisions are very, very hard. And so to play the game the way that I play it for this long is an absolute blessing."Dawkins was a six-time All-Pro and made nine Pro Bowls, including last season as an alternate.As a member of the NFLPA executive committee, Dawkins pushed for new league rules that limited full contact during camp and also in the regular season. He credited those changes for keeping him fresh at the beginning of what turned out to be his final season, which he played a year after laboring through sprains to both knees.
Long-time NFL safety announces his retirement
Long-time NFL safety announces his retirement
April 23, 2012, 5:27 pm


text:
Even A Former Justice
Has to Show Some Skin to Sell Books
By William Boardman
Supported News is the Publication of Origin
for this work. Permission to republish is freely granted with credit and a link
back to Reader Supported News
Non-Justice Gypsy Rose Lee by (operagloves.com)
Some Public Service
Merits Eternal Ingratitude
Sandra Day O'Connor, 83, retired as a Supreme Court Justice but still active on the judicial and book tour circuits, has been teasing the rest of us for years with hints and winks and suggestions that she may feel badly about being the deciding vote in the 5-4 Supreme Court decision that gave the country President Bush and all that he wrought over two disastrous terms.
In January 2010, O'Connor was asked whether Bush v Gore was the right decision and she danced away like this:
"I don't know. It was a hard decision to make. But I do know this: there were at least three separate recounts of the votes, the ballots, in the four counties where it was challenged and not in one of the recounts would the election have changed. So I don't worry."
Maybe Electing Bush
President Hurt the Court's Reputation
In the summer of 2012, O'Connor acknowledged declining public approval of the Supreme Court, and the widely held public belief that the court's decision in Bush v Gore was based on the Justices' political views. O'Connor acknowledged the case was "a tipping point," but did not tip her hand beyond that.
In March 2013, on Fresh Air, O'Connor responded coyly to a reporter's claim that she had admitted to regret for her Bosh v Gore vote:
" Well, I don't know why he said that. I've not said that myself, and it's not anything I would want to weigh in on. There's no point in my, at this point, saying I regret some decision I made. I'm not going to do that."
The reporter persisted, asking, "So you say you never really said that?"
"I hope I didn't," O'Connor answered, keeping hope alive with a masterful non-denial denial.
Tell Me, Justice
O'Connor, What Were Your Feelings About Bush?
On another program around that time, another reporter called O'Connor's attention to a photograph ff her and other justices sitting around looking bored while waiting for the 2001 Bush inaugural to start. The reporter, Rachel Maddow, served up a softball that let O'Connor slip away from any 20/20 hindsight. Maddow asked O'Connor how she felt that inauguration day:


text:
Luniz – I Got 5 On It
Unfortunately we don't have the LYRICS for this song. You can leave a comment below.
And buy the album or song if you wish to support the original artist and visit their Official Site.
[LYBIO.NET Becomes A Reading Discovery Layer.]
The Accurate Source To Find LYBIO's Discover Reading Content."
Filed under Hip Hop by on Sep 4th, 2011.
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Don't SnitchSubmitted by LifeIsABeach (not verified) on Mon, 12/09/2013 - 9:58am.
I'm not surprised as people worship wickedness and support it with such wicked slogans as "Don't Snitch". They walk around wearing their badge of dishonor saying I'm not a snitch. Thou shall not bear false witness.....means don't lie about your neighbor. Not telling something you witnessed is a lie of ommission (withholding the truth). »
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text:
Itz quite coooooooooool
very gud
Hi Tim! I finally got your card. I think fixed the spam problem. I had to send this "thank you"...
You sure know how to lift a friend.
thanks for the admiration, but im soon to be married with someone a care deeply of for many...
Thank you Eric and Pauline for my birthday card.sorry not taken a photo of Rochdale steps, trees in...
Thank You Dear. Wish you the same
dear CO Uncle thanks for the photos that u send me . I loved them so much n i liked d Teddy Bears...
These smileys will say thank you just the way you want them.
Say thanks with these cute hug...
Send this nice and cute ecard to say thank you.
Send this thank you ecard and show your gratitude.
Just a note to say, thank you. Send this ecard to your near and dear ones.
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A thank you card for a great person who is different and makes a great input on your life.
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Our report published in March 2014, Organic farming: how it stacks up, looks at the economics of organic farming.
All farmers, whether organic or not, need to monitor their finances. Many like to glance over the fence to see how their neighbours are coping with changing times.
How would I fare if I converted to organic farming or decertified? Will the prices cover the costs of production and leave a decent margin? And how will changing CAP payments, volatile input costs, the effects of climate change and emerging export markets change the picture?
To help with these decisions we have commissioned an analysis comparing how organic and non-organic farms have fared over the past six years. What is the overall pattern and what factors have most affected their bottom line?
The headline news is good for organic farmers.
The average annual farm business income (£/ha) over the six-year period shows that organic enterprises are outperforming non-organic, in some sectors to the tune of almost £80 per hectare per year. Even between 2008 and 2011, when UK organic sales were in decline, organic farming remained more profitable than non-organic production for most farm types.
Download your free copy of the report which includes full data and commentary on:
In March 2014, we arranged two events with Abacus Organic which examined how CAP reform may affect the future for organic farmers and how well organic farms have been performing financially over recent years. The presentations below provide detail on how CAP reform and the proposed exemptions for organic land from the greening measures under pillar 1 may favour organic farmers financially. The less obvious benefits of releasing capital and assets by converting to organic farming are also examined in depth. A financial appraisal of different organic farming systems and how they compare to their non-organic equivalents completes the picture.
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Organic Market Report
You might also be interested in our latest Organic Market Report. Published in March 2014, it reveals that sales of organic products in the UK grew by 2.8% in 2013, a decisive return to growth after four years of contraction.


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Follow Desperate Housewives
Doris Roberts on Desperate Housewives
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Its marie from everybody loves raymond
Did Jill really say that about Bethenny? You've got to be kiidndg. SHE has the nerve to talk about Karma? Jill, if this is how you react to seeing someone you once loved in such obvious pain, I think you're the one who better be worried about karma honey.Bethenny, keep smiling We love you!
I don't know how Lynette can be so determined to go back to her husband after their short separation then he's living with someone, won't she hate him later on for that? I don't think I could forgive my husband knowing he lived with someone and loved that person. Lynette forget about him and find someone else
- Photo Credit:
- Show:
- Desperate Housewives
- Episode:
- Desperate Housewives Season 8 Episode 20: "Lost My Power"
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- Gabrielle Solis
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- Desperate Housewives Photos, Gabrielle Solis Photos
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- Los Angeles, CA
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Desperate Housewives Season 8 Episode 20 Quotes
My husband's an alcoholic so I can only drink at work.
- Permalink: My husband's an alcoholic so I can only drink at work.
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Lynette: Huh. That's weird.
Tom: What?
Lynette: I have an "Isn't that Ironic?" app that usually buzzes when people say stuff like that.
- Permalink: Huh. That's weird. What? I have an Isn't that Ironic? app t...
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Via del Drago | V+ | ||
300m, 9 pitches. A classic route which takes a logical line up predominantly excellent rock. The long traverse is delicate but well protected (and also avoidable) and the upper pitches provide enjoyable and technical corner climbing. Recommended, even if the perhaps excessive pegging on some pitches detracts somewhat from the route's original 'dragon-like' daring. Start in the centre of the face on black rock, ascend rightwards up a small ledge to reach a small corner. 1) IV+, 25m. Climb a series of corners rightwards then exit right to a good belay on a small platform. 2) IV+, 30m. Ignore the yellow corner above and step left, then enter a chimney. Climb the chimney passing a chockstone near the top to move right onto a ledge. Belay below a crack. 3) III, 20m. Follow a ramp right over easy ground then move up and left to a thread belay. 4) V+, 30m. Continue left, then move right, level with a ledge on the left. Climb a slab direct for a few metres then ascend diagonally left. Continue left past a flake to a good belay. 5) V+, 25m. Move left then climb a crack direct before an exposed traverse left leads to a belay on a ledge. 6) V, 35m. Continue left making an initially delicate traverse following a horizontal yellow crack (exposed but well protected) to then ascend to the base of a pillar. 6a) II, 35m. For an easier variant, ascend a small corner above the belay to reach a large ledge and follow this easily left (II) to rejoin the first variant. 7) IV+, 40m. Climb a black crack to the right of the pillar leftwards, then from the top of the pillar continue in the same line following a flake to a belay below a crack. 8) IV, 30m. Climb the crack above then exit left, pass the base of a small corner and climb a second crack to a belay on the left, just left of a small black roof. 9) V+, 25m. Climb the large black corner above, pulling through an overhang near to top to reach a belay on a ledge. 10) V, 30m. Follow the continuation corner above keeping mainly on the right wall then exit right onto a ledge below inclined rock. 11) IV-, 45m. Climb the slab above from left to right then enter a chimney. Follow this to a thread belay to the right of the prominent yellow roofs. 12) IV-, 20m. Follow a crack direct or slightly left to exit onto a large ledge just right of a yellow tower. © ROCKFAX Submit a new photo of this climb. | |||


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I live in Norway with my husband and baby son. We love traveling around the world.
Archana has completed online and offline ID verification.
It was a pleasure to host Archana and her family. They were very friendly, courteous, and overall perfect guests. I would highly recommend Archana and her family and would welcome them back anytime.
Archana and her family are very nice people. Polite, tidy and nice. It was a pleasure to host her. She was very communicative throughout the reservation and arrival process and left the flat in an immaculate condition. I would highly recommend her as a guest and would welcome her back
All-star guests. Cool, clean, friendly, positive vibe... You'd do well to rent to Archana and her guest. They help make airbnb a happy place!


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Get double the action and double the fun with Slingo Supreme 2 Deluxe, the all-new entry in the slingoriffic series. Slingo returns bigger than ever with two times the power-ups, two times the mini-games, and two times the awards. Play up to 10 million combinations in four modes with 32 power-ups featuring Slingo Vision and Treasure Chest, plus new ones including Don't Stop and Echo Slingos.
Try the full version of Slingo Supreme 2 Deluxe for free!
Slingo Supreme 2 Deluxe features


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Staybridge Suites Portland - Airport
Quick Info
- Check In: 4:00 PM
- Check Out: 11:00 AM
- Minimum Check-In Age: 21
- Late Check Out Available
- Parking: Free
- Print Fact sheet
- Chat Online
Hotel Highlights
- On-site Guest Self-Laundry Facilities
- Housekeeping Full Service
- Complimentary Breakfast Buffet
- Standard Wireless Internet service is free for all members
- On-Site Fitness Center Available
- Complimentary Shuttle Available
Guest Reviews
Overall Experience
Nice hotel but a bit noisy
05 marzo 2015
Very little sound proofing, so late arrivals and early departures (or just talkative neighbours) can be very disruptive. I'd advise you to bring earplugs!
Solid Recommendation
03 marzo 2015
The staff were warm and welcoming, the hotel feels energetic and welcoming. The rooms are spacious. The beds and pillows comfortable.
Dont miss the customer appreciation dinners tue-thurs.
I stayed for business. This is a solid hotel and so is my recommendation.
Comfortable and a Great Value
26 febrero 2015
I choose this hotel primarily based on the online reviews (top ten for reviewed Portland hotels at any cost). There are more than a dozen options if you want to stay at a hotel by the Portland airport and I was booking with somewhat short notice for a trip out of town and wanted to find something that was newer (which it appeared to be), reasonably priced, with an indoor pool and which would take pets.
The three things that stood out were how clean the rooms were, how friendly the staff was and what a great value you get.
The rooms were a very good size (got a two queen bed room with a living room and a bedroom). They had a kitchen with a fridge, stove and microwave oven. They looked newer and didn't show much if any wear (that pretty much goes for the entire facility).
As far as value, the room was priced very similarly as other hotels near the airport, but none which came close as far as reviews or amenities (or pet policy). The room comes with breakfast every morning (and not just Danish, juice and coffee but a pretty impressive spread). They had several kinds of coffee, several kinds of juices, oatmeal, cereals, eggs, sausage, a Belgian waffle bar, muffins, and yogurt. Pretty much anything you would need to start your day, but they also have a Starbucks relatively nearby as well as a Dutch Brothers Coffee. You also get free wifi (which I always assume comes with the room but you never know), free office center and free laundry room.
The staff was very friendly. Regardless of what time you went to the front desk, they seemed happy to help and they were consistently friendly (from the 6AM shift to the 10PM shift, which isn't always the case).
Would definitely recommend if you are looking for a hotel in Portland by the airport.
Nice place to stay
23 febrero 2015
Stay here often when visiting Portland. Good service and I can count on the service to be repeatable. Staff very friendly and appreciated my status as member.


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Cosy Cottage in the Heart of the Cotswolds
From $155 / night
Hide Property Details
- 3 bedrooms
- 1 bathrooms
- Sleeps 6
- Cottage
Description from the owner
This Self Catering Holiday Cottage is located in the picturesque village of Bibury.
33 The Square, was built in the 18th Century and is a Grade II listed Cotswold stone cottage with lots of charming features. It is located in the beautiful village of Bibury, right in the heart of the Cotswolds, a short drive from Cirencester and Burford.
Cosy Cottage in the Heart of the Cotswolds
Cirencester, Heart of England, England
What's Nearby:
Additional Location Information
The Village of Bibury is set in beautiful countryside on the banks of the River Coln, it is host to the famous Arlington Row. The cottage is perfect for groups or families wanting a relaxing countryside break, a great central base for exploring the surrounding countryside, Cotswold towns and villages.
- Linens Provided
- Fireplace
- Central heating
- Washing Machine
- Towels Provided
- Alarm Clock
- Internet Access
- Parking
- Stove or Oven
- Dishwasher
- Refrigerator
- Paper Towels Provided
- Freezer
- Microwave
- Grill
- Toaster
- Television
- Stereo or CD player
- Books
- DVD Player
- Satellite or cable TV
- Outdoor Dining Area
- Garden
- Pet friendly - no
- Smoking allowed - no
- Suitable for children - yes
- Suitable for the elderly - yes
- Wheelchair accessible - no


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Product Description
Available while supplies last.
Sullivan Chandelier
Gently curving double arms arch towards stylish, flared shaped opal white glass. The qualities of the opal glass provide a pleasantly diffused light for daily use as well as for special occasions.
- LED offering up to 800 lumens (60 watt equivalent) with full range dimming
- Includes an easily replaceable LED bulb (120V)
Voltage: | 120 volt | |
Weight: | 23 Pounds | |
Installation: | Dry Locations [Explain] | |
Listing: | Eco-Friendly, LED, Energy Saving, Energy Efficient |
Bulb is included free
Bulbs are not eligible for replacement
Bulbs are not eligible for replacement
Questions & Answers
There are no questions from the community yet
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What's this?Additional Details
Mfr Part # | |
Sku | |
Group # | 1506663 |
Category | Chandeliers - 89 |
Style | Transitional - 15 |
Sales Rank | 1000000 |
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Safe Eyes 6.0
Safe Eyes 6.0 : Video Report
The videos section of the activity report shows a thumbnail of every video blocked and of every video viewed without blocking, along with the supplied description. Clicking the thumbnail jumps right to the video itself.
13 / 32
Bottom Line
Safe Eyes 6.0 focuses more on what the family likes to do online than on how to control what they do, though it retains all its protective features. Its online activity reporting needs work, but it's still the best choice for families using both Macs and PCs.
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- 7 Anti-Aging Superfood Recipes - Fox News Latino
- Puerto Rico Bill Would Fine Parents With Obese Kids Who Don't Slim Down - Newser
- Sofia Vergara reveals a fan once asked if she was a transvestite - Fox News Latino
- Boy Sends 1,076 Valentines —to Every Girl in School - Newser
- Famosos que no te acuerdas fueron pareja - Telemundo 51
Nonmember comment from Kim
on Sep 6, 2012 at 4:12 PM
i've had orgasm headaches before.
the pain is horrible but they only last 3-5 minutes.
never knew it was a 'actual' thing.
Nonmember comment from Steph
on Sep 6, 2012 at 4:19 PM
None of those were really surprising to me. All of these things have been around and happening for decades, minus the cell phone since they still are fairly new.
Facebook comment from Lynnette Geter
on Sep 6, 2012 at 4:24 PM
Throw up? WTH that is sad. Fictional sexual escapades is a best seller and real information about human sexuality is gross? Not GETTING IT?
Nonmember comment from Katie
on Sep 6, 2012 at 4:57 PM
My fiancé has had climax headaches a few times and he started taking Zinc just any brand and they are gone.
Facebook comment from Christine Elder
on Sep 6, 2012 at 5:01 PM
people are too materialistic- give up sex for a cell phone
Facebook comment from Melinda McWilliams
on Sep 6, 2012 at 5:50 PM
I don't see any of this that I haven't already known LOL. I hope soon that someone will be able to come up with some stuff that I don't know. I have looked all this stuff up a few years ago.
Facebook comment from Natasha Coleman
on Sep 18, 2012 at 5:04 PM
I love orgasms. Who knew there was a day for it. I need more then just one day.
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text: 1538 Pebblebrook Tr Status: Off Market MLS#: 1652020 Contact Email this listing Share on Pinterest Share on Google+ Share on Facebook Share on Twitter Calculate Mortgage Print Flyer Get Directions Request a Showing Off Market 1538 Pebblebrook Tr, Sun Prairie, WI 53590 is no longer on the market or has been removed from the MLS. Similar to 1538 Pebblebrook Tr: Ask an Agent About 1538 Pebblebrook Tr * Required fields Send Your Question × Monthly Payment Estimator Price: Down Payment: % Loan Amount: Loan Type: 30 Year Fixed 15 Year Fixed 5/1 ARM Interest Rate: % Est. Tax & Insurance: % ($/mo.) × Email a Listing 1538 Pebblebrook Tr, Sun Prairie WI, 53590 From: Name: Email: To: Name: Email: Subject: Message: Take a look at this property I found on NicoleCharles.com: http://www.nicolecharles.com/homes/1538-Pebblebrook-Tr/Sun-Prairie/WI/53590/21708344/ Send a copy of this message to me Send Email