text:
- Dear Robin, Fantastic! The biggest gap in my family
information is the place of burial. Another cousin,
Trish Worthington Cobb, and I spend every spare minute
when we get together a couple of times a year going to
cemeteries and taking pictures of headstones--Trish is
the photographer: she takes the pictures, and I write
out the information. Actually to see my family
members' graves is like having my family with me. I
probably feel more strongly than some of our relatives
because I did not have the opportunity of even knowing
my generation of relatives in so many cases and never
had the chance to grow up with any of them. It's like
being an orphan and finally realizing that I have an
incredible family whose lives have spanned almost the
entire history of our country. I'll get an email off
to you as soon as I finish deleting my 200+ overnight
bulk mail measures so that I can restore my space!
Thanks so very much, Carol
--- Robin Nichols <nicholra@...> wrote:> Hi Carol and Louise,
=== message truncated ===
> I have been reading your emails back and forth on
> the Nichols line and thought I would jump in. My
> husband is a descendant of Ardens son Silas (James
> brother). I have been trying to find as many
> cousins from Ardens various descendants. Carol, you
> are the first I have found from James' line. If you
> are in my area (Bell Arthur) and want a tour of the
> numerous Nichols cemeteries I will be glad to take
> you around. Send me a private email and I will give
> you my phone number. James and Emily as well as
> several of their children and grandchildren are
> buried on/near our farm. I will be glad to share my
> Nichols information with you. Louise I have not
> forgotten about those pictures you want.
> Robin
> nicholra@...
> Robin Avery Nichols
> Main surnames AVERY, BYRD, SASSER, DUDLEY,
> >>> champright@... 06/03/03 06:11PM >>>
> Hi Carol,
> You are so right about these families and more. I
> know the
> Worthingtons lived next door to my Cooper family and
> my mother knew
> them well. There are many small cemeteries on the
> old Nichols Road
> (It is the long road that goes through Bell Arthur,
> just next to
> Winterville--I think it is call Bell Arthur Road
> now). Robin
> Nichols lives on that road and I think her line is
> also James
> Nelson Nichols. She is very interested in her
> husband's family
> line (Nichols). I don't know how much you know, but
> Arden Nichols
> owned all of the land along that road in 1850. When
> his sons
> married he gave them each a farm along Nichols'
> Road. And guess
> what? There are Nichols cemeteries all along the
> Road and Robin
> has pictures of them. One of the grave yards is the
> Worthingtons.
> Most of the grave yards are inclosed in a iron fence
> and you can
> see them from the road. They are taken very good
> care of. Robin
> takes care of two herself. My sister, Faye, and I
> went to Bell
> Arthur/Winterville and just happened to be there
> during Sept 11,
> 2001. We were there only two days, but we saw many
> of the graves
> that I am talking about. We also know where John
> Slaughter and
> Mary Worthington are buried. We saw it somewhere on
> the internet
> and we went looking for it.
> In September there was a field of corn (with under
> growth) there
> and you had to go through there to get to the grave.
> I had a flash
> light and started through (it was getting dark). I
> went about half
> way when the fellow came out and told us that there
> were rattle
> snakes in there. Boy did I come out fast. He said
> the best thing
> to do was to come back after harvest and the field
> would be
> cleared. Of course, I live in California and didn't
> get to come
> back. I just found the article below on the
> internet. It is
> greee-at!! Must go--talk later Louise
> While researching some Pitt County family members I
> happened across
> a family cemetery covered in thorny thickets in the
> middle of a
> tobacco field on Hwy 903 2.1 miles west of
> Winterville, NC. These
> are the names I was able to capture.
> Mary A.S. Slaughter
> Mar 14 1861 - Aug 27 1861
> Catherine Slaughter
> 1858-1876
> John Slaughter
> 1816-1886
> Rebecca Slaughter
> 1866-1886
> Nancy S. Norris
> 1848 - 1875
> These are the actual dates I removed from the
> tombstones, there are
> possibly more in the thorny thicket but I was able
> to get these
> names without sustaining too much injury.
> There is a similar listing of Slaughters that has
> now been proven
> to be incomplete compared to what I found. There is
> also a (DALE)
> family cemetery across the road from the Slaughter
> Cemetery that is
> also in the middle of the tobacco fields. I suggest
> all researchers
> plan fall trips to Pitt County for the cemetery
> searches because
> the tobacco fields seems to be where these small
> family cemeteries
> are at. In the middle of the 3 family home sites I
> went to the
> other day all 3 had fresh plowed fields exposing the
> cemeteries
> which were tall thorn covered green area's that were
> 100%
> surrounded by summer tobacco which would hide them
> during the
> summer months making access impossible. October
> seems to be the
> tobacco harvest thereby making the searches easier.
> The (SHIVERS)
> cemetery at Winterville information is correct as
> posted except
> that the cemetery is on the Shivers home site and is
> still owned by
> Shivers.
> James Shivers
> Shivers Family Researcher
> shivers@...
> Further lookups and information can be
> bargained,purchased or
> beaten out of me.
> --- Carol Singh <csinghworthington@...> wrote:
> > Dear Louise, Thank you so very much. You have my
> > Cousin Linda in your line. Nichols women married
> > Slaughter and Worthington men, and I am related to
> the
> > familly through those lines. I believe that
> > Willoughby's are related to the Pippins, and
> Pippins
> > married into the Cherry line--my maternal
> > Cherry-McGowan families. They also married into
> the
> > Cannon family, which I am related to both from the
> > paternal Dail line and from the maternal
> Worthington
> > line. I've just gotten off work (evening shift),
> and
> > my notebook is in the car. I'll read through
> > everything you've sent very closely and get back
> with
> > you tomorrow on the connections I've found. For a
> > beginning, I believe that your Fannie Slaughter
> was
> > the daughter of Mary Polly Worthington and John
> > Slaughter. Mary Polly Worthington was the daughter
> of
> > my great, great, great, grandfather Spire
> > Worthington--I think I'm right about the number of
> > "greats" but could have left out one or two. Are
> you
> > familiar with the Worthington line? Later, Carol
> > --- Louise Champion <champright@...> wrote:
> > >
> > > This is Linda and my genealogy outlines.
> Linda's
> > > great grandfather
> > > (James), and my great grandfather (George) are
> > > brothers. They are
> > > sons of Arden Nichols.
> > >
> > > Direct Descendants of James Nelson Nichols
> > > 1 William Nichols and Lydia Briley
> > > 2 Arden Nichols and Edith Tyson
> > > 3 James Nelson Nichols b:
> > > September 14, 1831
> > > in Nichols, Hemby Cemetery,
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http://calendar.yahoo.com - Dear Robin, Thanks so very much. I'm thinking, though,
that it must be somewhere on Carney property. Grandpa
Alp (Alfred) bought plots in Greenwood Cemetery in
1934 when Grandma Jennie died--June 1934. Before then,
McGowans were buried in cemeteries where other
relatives were buried(Cherry Hill, Brooks, etc.) and
in church cemeteries. However, I did not find Grandma
Jacky in Red Banks or Reedy Branch, and with no death
certificate and Greenwood incomplete on line, I
assumed that Grandpa had had her buried in Greenwood
and that I would run across it there one day by
walking through and searching each grave. Since
Grandma Jacky and her sister, Jennie L., were very
close, I am not surprised that Jacky was buried in her
sister's husband's family cemetery and even less
surprised when I realized that Carney's maternal line
was Hatton--the McGowan maternal line. McGowans liked
to be together in death as well as in life; that much
I did know and was surprised not to find all of them
together. The Hardee (my Hardee-McGowan-Worthington
line) maternal line was also McGowan, and McGowans and
Hardees are buried together, but I didn't find Jacky
with them either. People are so interesting! Later,
--- Robin Nichols <nicholra@...> wrote:> Carol,
=== message truncated ===
> I have not heard of the Robert Carney Burial Ground.
> I'll keep my eyes
> open. I am also a member of the Pitt County Family
> Researchers and
> Roger and Bill are wonderful sources for
> information. If the cemetery
> is still out there, I am sure Bill will run across
> it one day. My
> husbands materal grandmother was also from the
> Mcgowans. I have done
> some research on them, but not as much as I would
> like. I'll look
> through my Mcgowan stuff for any of these names.
> Robin
> >>> csinghworthington@... 06/06/03 11:09AM >>>
> Dear Robin, Hi! One of our members sent me yesterday
> a
> copy of my great grandmother's death certificate
> from
> the Pitt County Courthouse. The death certificate
> says
> that she was buried in the Robert Carney Burial
> Ground. Jacky Ann McGowan died June 18, 1925 at the
> age of 75. The death certifcate filled out by my
> grandfather,son-in-law of Jacky Ann, noted also that
> Jacky Ann was a widow. I've never been able to find
> out more than that her consort was a cousin, William
> McGowan. Jacky had at least four cousins named
> William
> Mcgowan. As far as I can find all but William George
> McGowan died before 1920--I'm guessing here, though,
> because I was relying on census records. Roger
> Kammerer from the Pitt County Family Researchers let
> me copy some McGowan records he had, and that
> informant said that William K. [Kinsey] McGowan
> never
> married. Of course, William Kinsey, son of William
> and Martha Ann Moore, was born around 1852, and I
> doubt that Roger's informant in the late 1999's knew
> this William personally. He may have the facts
> correct, or he may not have known if this William
> married because this William may have chosen not to
> reveal the fact. One of my cousins wrote that Latter
> Day Saint records show that this William was indeed
> the William who was Jacky Ann McGowan's husband. I'm
> hoping that Jacky's tombstone might tell me more.
> Her
> death certificate did not give either her parents'
> names or the name of her deceased husband. Daughter
> Jennie Secession McGowan gave her father's name upon
> her marriage only as William McGowan. I was
> wondering
> if you or any of our members has heard of the Robert
> Carney Burial Ground or cemetery. It is not online,
> and Bill Kittrell (also a member of the Pitt County
> Family Researchers) says he is not familiar with it
> but will look into it. The 1850 census shows Robert
> Carney and Elizabeth "Betsy" Hatton as the parents
> of
> Robert Carney who married Jennie L. McGowan, sister
> of
> Jacky Ann McGowan. Martin Moore, I see from the 1850
> census, was married to Cynthia Hatton. Martin Moore
> was the father of Perlina [Paulina] Moore, mother of
> Jennie L. and Jacky Ann McGowan. Jacky's daughter
> Jennie Secession McGowan was named for her Aunt
> Jennie--I've seen Jennie's name spelled "Jeanett,"
> but
> Mama said it was Jennie. The Carneys lived in
> Greenville Township North of the Tar River.
> According
> to some of my relatives, Jennie grew up in the
> Pactolus Area; she attended Parker's Chapel Church.
> As
> for the Robert Carney Burial Ground, I do not know
> if
> it was simply a family cemetery located on the
> family
> property as many such cemeteries were or if it was
> on
> land given by the family for a cemetery, as Cherry
> Hill was. I also do not know if this cemetery is
> conveniently included in one of the books at Joyner
> Library on Pitt County Cemeteries. When I was there
> last month, I had never even heard of the Carney
> cemetery, so of course I didn't look it up. I had
> assumed that most of my relatives were buried in
> Greenwood because it was the cemetery used by my
> immediate family until the death of my Uncle Poe
> Worthington in 1968. Aunt Doris did not know that
> Grandpa Alfred (1871) had bought six cemetery plots
> in
> Greenwood, intending three of them for his children,
> so he was buried in Pinewood. Like a poor soul on
> "Who
> Wants to be a Millionnaire," I am now polling the
> audience. Thanks for any information on this
> cemetery
> or on William McGowan obituaries. Later, Carol
> --- Robin Nichols <nicholra@...> wrote:
> > Louise,
> > My husband's grandparents use to own it. I'll
> have
> > to ask my father-in-law about previous owners. I
> > have a picture of how it looked say over 30 years
> > ago. It has had a lot of remodeling done to it
> over
> > the years. The old Nichols two story which is on
> > the same road has also had a lot of remodeling. I
> > have not found an old picture of it yet. Aunt Dot
> > Nichols lives there now, maybe she has an old
> > picture of it. It was originally owned by Robert
> > Nelson Nichols (Silas Nichols son). Back on Bell
> > Arthur Road (formerly Nichols Rd.) there is
> another
> > Nichols house. I think there is an old picture of
> > it "Chronicles of Pitt Co." My father-in-law owns
> > the farm around it and he and my brother-in-law
> had
> > it remodeled and my brother-in-law still lives
> > there. There use to be a two story (Nichols)
> house
> > beside my house, but a tornado in the early 70's
> > destroyed it. This house I think belonged to
> Heber
> > Leon Nichols (Thomas Arden Nichols son). Thomas
> and
> > his first wife Emily and two of there children are
> > buried across the road.
> >
> > Robin
> >
> > >>> champright@... 06/05/03 10:23AM >>>
> > Hi Robin,
> >
> > This is great!! A new cousin just around the
> > corner, but I think
> > it is Linda that is the descendent that lives
> around
> > the corner
> > from you. The "James Nelson Nichols house" is in
> > the "Chronicles
> > of Pitt Co." Many of Arden's sons could build a
> > house. I wonder
> > if James built the house in the book.
> >
> > You should go down and see that house on Nichols
> > Road that I was
> > telling you about. It is real cute. It looks
> like
> > it was built
> > about the same time as the one in the book. The
> > road is just off
> > of Bell Arthur Rd and it is called Nichols Rd.
> Just
> > behind that
> > house (about 50 ft) is a Strickland cemetery
> > (Jeremiah Nichols b.
> > 1871) is also there. Jeremiah was one of Arden's
> > sons also. My
> > family (George Nichols) would come over to visit
> you
> > aunt Christina
> > (Little Bit) and all of the Nichols families
> there.
> > They knew Bell
> > Arthur very well. But George never lived there
> > after he got
> > married. Louise
> >
> > --- Robin Nichols <nicholra@...> wrote:
> > > Hi Carol and Louise,
> > >
> > > I have been reading your emails back and forth
> on
> > the Nichols
> > > line and thought I would jump in. My husband is
> a
> > descendant of
> > > Ardens son Silas (James brother). I have been
> > trying to find as
> > > many cousins from Ardens various descendants.
> > Carol, you are the
> > > first I have found from James' line. If you are
> in
> > my area (Bell
> > > Arthur) and want a tour of the numerous Nichols
> > cemeteries I will
> > > be glad to take you around. Send me a private
> > email and I will
> > > give you my phone number. James and Emily as
> well
> > as several of
> > > their children and grandchildren are buried
> > on/near our farm. I
> > > will be glad to share my Nichols information
> with
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FULL RESOLUTION - 968x726
News » Published 13 hours ago
Is this Lindsay Lohan the Actor?
Share your thoughts on this image?
5th March 2015 @ 09:45am
Lindsay Lohan has been spending a lot of time in London lately and she really seems to be loving it there
21st January 2015 @ 04:31am
Lindsay Lohan according to media reports from is filming a commercial with car insurance company Esurance that will make its debut during the 2015 Super ...
31st October 2014 @ 02:42pm
There have been claims that Lindsay Lohan and Tom Cruise are dating and Lohan's people have addressed the rumours

subset: commoncrawl


text:
Every week there seems to be a new announcement regarding some game from a bygone era getting the HD re-release treatment.
The IncGamers Podcast goes robo-loco as Tim shoots many robots in, err, Shoot Many Robots, John tries out the equally metallic Twisted Metal and Peter wishes he at least had some robotic companions in the often empty world of Microsoft Flight.
Has Nvidia's response to the GTX 970 VRAM issue been adequate?


text:
Which bike is better if both are priced at 300-325, the Jamis is only once used but the KHS has a nice set of manitou forks. Help me out and let me know what yall think. Thanks.
Results 1 to 5 of 5
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La Terra Dei Sogni
- Continental Breakfast
- Swimming pool
- Restaurant
- Free High-Speed Internet
- Room service
- Wheelchair accessibility
TripAdvisor Reviews La Terra Dei Sogni Fiumefreddo di Sicilia
Travel Blogs from Fiumefreddo di Sicilia
Quiet day in Taormina
JOSH - OMG it is so warm I'm lovin it it is the most beautiful place with the most amazing VIEW I wish we could have a few extra weeks we went skiing were all so good especially ME LOL!!!! I love Sicily it's so quiet and about the skiing we did it on a VOLCANO WOW. ( Mum thought it would erupt when will came down hahahahahahah ):):):):):) DAD - another lovely day in Taormina. We went back to the markets to get some more ...
Storm of the century & centuries-old salt & stone
... homelike than a fabulous Swiss village or seaside Sicily. There's homework to be done, uniforms to wash, Christmas thank you notes to write. There's six months ahead of us -- to chart the rest of our course during this now NEW year of both the every day and the extra special.
Happy New Year, friends far and near. We are thankful for your place in our lives and look forward to time together to share more stories.
Greek Theatre
... telling in a culture. In most cases, where the most power lies, the most rich culture lies also. For a culture to invest so much in theatre productions does not diminish a society for focusing on non-necessities, but instead shows the power and influence of a society. It shows great power that a society had the leisure and luxury of investing in the arts. The Greeks started this ...
Taormina to Palermo
... but tonight was very interesting. We had no idea what we had ordered for dinner, it was hard to get an English translation but all in all it wasn't too bad. Neil and I really enjoyed our steaks with mushrooms and John and Maxine enjoyed their seafood pasta!! We had lots of laughs over the ...
... Chalcis in Euboea and Naxos in the Cyclades. We then headed back for a gelato and coffee at G&C before our last walk down the mall. Neil bought a "man bag" and new wallet!! Shock horror!! We had a wine and watched the passing parade on our last night in Taormina. Tomorrow we head for ...


text:
Three Cuts: Braves bats help clinch series win over Angels
JUN 16, 2014 1:45a ET
ATLANTA â After a slow start at the plate (two hits and no runs through five innings), the Atlanta Braves scored four runs in the sixth inning to take a 4-3 lead over the Los Angeles Angels, and never looked back on Sunday. The Braves added runs in the seventh and eighth, and held on to win 7-3. Here are three observations from the series-clinching victory:
1. The Braves masterfully answered the Angels' big inning with a bigger, better version
Through the 67 games prior to Sunday's, opposing hitters have only been able to muster 57 hits while batting .244 in the third inning against Atlanta. Outside of the ninth inning this season (32 hits, .171 batting average allowed), Braves pitchers have enjoyed hurling in the third frame as much as any other.
That wasn't the case Sunday night.
The Angels opened the third with four straight hits: a solo blast from Mike Trout, and then Albert Pujols, Josh Hamilton and Howie Kendrick followed with singles. Kendrick's drove in Pujols for the third run of the game.
By the end of the third, Los Angeles led 3-0, and had accumulated eight hits. The Braves had just one, a Freddie Freeman double in the first. After five innings, the Angels had 11 hits while the Braves could muster only two. Everything was going Los Angeles' way.
Then the Braves had a huge inning of their own to one-up the Angels.
After Jason Heyward popped out to the shortstop to start the sixth, B.J. Upton walked. Then the floodgates opened. Freeman, Evan Gattis, Justin Upton and Chris Johnson each singled. Gattis drove in B.J. Upton, Justin Upton drove in Freeman, Chris Johnson singled, and then Tommy La Stella doubled to drive in Gattis and Justin Upton.
"I think that's some kind of record," manager Fredi Gonzalez joked about the five consecutive hits Atlanta assembled in the sixth inning. "We hadn't seen (Hector) Santiago at all. The third time through the lineup, I think we had a better read on him. We started seeing better at-bats."
The Atlanta hits didn't stop after Santiago was chased from the game, however. The Braves added two runs on three hits in the seventh, and one run on two hits in the eighth. What might be more impressive than the Braves' seven runs in their final three innings is how they scored them.
Only one run, Heyward's solo shot in the seventh, was plated with the long ball. In a season where everything seems to happen because of a home run, the Braves scored, and largely won, as the result of two bases on balls (one intentional), eight singles, a double, a stolen base, two wild pitches and a sacrifice fly.
That, Braves fans, is called small ball.
It's also called a series win, Atlanta's first since a three-game sweep of the Miami Marlins from May 30-June 1, and just their third in their last 10. The win also pushed the Braves back into sole possession of first place in the NL East at 36-32, one game ahead of both the Marlins and Washington Nationals.
2. Good riddance to Trout & Co.
The Angels boast one of the most feared trio of hitters in baseball in Trout, Pujols and Hamilton. Between the three, they have a combined 768 career home runs (Pujols 508, Hamilton 185 and Trout 75) and four MVP Awards (Pujols in 2005, '08 and '09, and Hamilton in 2010). In Trout's first three seasons, he finished in second place in the MVP voting twice. Take away Pujols' three first-place finishes, and he has seven other top five MVP showings.
Oh, and both Pujols (2001) and Trout (2012) were Rookies of the Year.
Prior to the game, Gonzalez said the most dangerous Angels hitter might not be one of those notorious three.
"(Erick Aybar) may be more dangerous than the other guys," Gonzalez said. "Because he's going to put the ball in play, he's going to get on, he's going to steal you a bag."
Aybar got the scoring going for the Angels in the second inning with a solo shot, as if he had heard Atlanta's manager give praise hours earlier. But then Los Angeles' big three took over.
Trout went 3 for 5 with a home run, while Pujols and Hamilton were both 2 for 4. Combined the trio put up a 16-for-42 (.381) series with three home runs and 12 RBI.
"Do these guys hit every inning?" Gonzalez said he asked bench coach Carlos Tosca during Saturday's game. "What's going on over there? Are they hitting out of order? It seems like they hit every inning: Trout, Hamilton and Pujols."
Even though the Braves took two of three games from the Angles, Gonzalez, and likely the Braves pitching staff will be happy not to have to see these Angels again in 2014, unless a World Series ring is on the line.
3. Gattis, La Stella continue raking, streaking
At the beginning of June, you would have gotten some pretty steep odds in Vegas if you had said the two players leading the Braves in base hits after the first two weeks of the month would be Gattis (20) and La Stella (19).
That's exactly how it's played out, though, and the Braves skipper said his team has gotten exactly what it expected from La Stella.
"I think La Stella thinks there's another league (to advance to) other than this one, the way he carries himself," Gonzalez joked after Sunday's win. "It doesn't surprise me that he feels this comfortable right away. He's doing what we thought he was going to do, swinging the bat and putting the ball in play. Our minor-league, player-development guys gave us a great scouting report on him."
La Stella said it's nice to have started his career in the majors on a good foot, but didn't have any expectations of a statistical manner.
"It's not always going to be like this," La Stella said of his early success. "So I'll take it when I can get it."
La Stella is currently enjoying a six-game hitting streak and has hit safely in 12 of his last 13. Counting Sunday, he's provided the Braves with nine multi-hit efforts in 16 games with the team. Over his last 13 games, La Stella is batting .447 with two doubles and six RBI.
With Gattis' 2-for-4 night Sunday, he's now hit safely in 15 straight, the longest active streak in the National League. During the streak, Gattis is hitting .417 with three doubles, seven home runs and 18 RBI.
As Gattis and La Stella think about their hitting streaks, the Braves will try to focus on beginning a new streak of consecutive series' wins. Atlanta welcomes the Philadelphia Phillies to town Monday for three games. The Braves took two of three from the Phillies in April in their first meeting with the divisional foes.


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My wife & I just returned from our stay at Tambor Tropical. After several very stressful years of schooling & work, we desperately needed a time of quiet and refreshing in a beautiful place. We had found Tambor Tropical on-line a number of years ago, and has always dreamed of going there because it looked so pretty. Honestly, we had prepared ourselves to be disappointed because how could a resort in real life look as good as the pictures on the web site? When we arrived, we were blown away! It was everything we had hoped for and more. Everything about the resort was perfect. From the beautiful teakwood rooms and wrap around decks, wonderful food & drinks (all made with fresh ingredients) , friendliest staff we have ever met, all nestled in the beautiful natural surrounding. The Mango Daiquiris were the best!! We will come back to Tambor Tropical, every chance we can. It was the best vacation our our lives! Martin & Debi Warner, Fort Collins, CO
- Official Description (provided by the hotel):
- Tambor Tropical is an intimate boutique hotel on the Pacific Ocean that offers guests a private and luxurious vacation in the less traveled Playa Tambor on Costa Rica's beautiful Nicoya Peninsula. Relax in the comfort of one of our 12 spacious beachfront suites, swim in our gorgeous mosaic-tiled pool, indulge in massage treatments, enjoy the cuisine of our full-service restaurant, and explore the nearby jungle via many guided adventures. ... more less
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- Also Known As:
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- Tambor Tropical Playa
- Tambor Tropical Hotel Playa


text:
Demetria McKinney - Work With Me
Artist: Demetria McKinney
Producer: Michael Snoody
Album: Officially Yours EP
Avg Rating: 3.3 ( 3 votes )
I can comfortably sit in front of my laptop and suggest you listen to one of our many newly-featured singles, but it won't mean much if you don't take the time to listen; Work With Me, people! For example, when I tell you to that Booth newcomer Demetria McKinney needs to be on your radar, please pay attention. Some might recognize Demetria from Tyler Perry's TBS sitcom House of Payne, but there is no acting when it comes to her music. Atop Michael Snoody's bobbing and swaying R&B production, Ms. McKinney displays a natural singing ability and comfort on the mic that proves she is more than just an one-dimensional entertainer. With her smooth, understated vocals Demetria explains to her boo that she will do whatever it takes to keep him around; with the passion in her voice, how can he say no? Work With Me will be found on the Fort Walton Beach, Florida native's Officially Yours EP, due out this fall.
Demetria McKinney R&B/PopIndie
Written by Lucas G. on 09/20/13
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Fallout 3
Question from JDawg36
How do I get to Muesuem of Technology?
I need help getting to the muesuem of technology i have gone everywhere and still cant find the place if anyone can help please i would appreciate it
5 years ago
have you found it yet??? It's been over a year.....
Close this question please.
4 years ago
If you'v found Rivet city already (bottom right corner of map) travel there, now go north and you'll find a metro station (Anacostia Crossing) go inside the metro travel the station util you get to the tracks then go north (left) and you will come across a door marked "Museum Station" go there. Now keep following the tracks until you find another station with raiders, you'll then have two exits both marked as "The Mall" one to the east and one to the west, go out the east one, once outside turn right and The Museum of Technology will be right there. There'll also be a few super mutants.
5 years ago
Go to the Washington monument (the big needle looking building) face towards a building in the distance with a dome on top, when your facing that way go to your right from the monument and walk towards that building with the dome and you will come across the museum of technology.
5 years ago
Have you found the museum of History?
If you have then just check across the open area and it should be almost directly across from the museum of history.
5 years ago
Have you found the museum of history?because the museum where you looking for is there close near and if you didnt found you can get there trouge the metro station georgethown
2 years ago
This question is open with pending answers, but none have been accepted yet
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You must be logged in to answer questions. Please use the login form at the top of this page.
More Questions from This Game
Question | Status | From |
How can i get to the safe in the museum of technology? | Open | logath |
Where can I find the technology museum safe key? | Answered | cob18 |
How do I add a comment, to an answered question? | Unanswered | Ninjaborg |
I Cant find DogMeat or anyone in the scrapyard? | Unanswered | krakenzx |
Why does the game keep crashing near the National Archives? | Open | TehderpGuy |


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PlantFiles: Italian Jerusalem Sage
Phlomis italica
Family: Lamiaceae (lay-mee-AY-see-ee) (Info) Genus: Phlomis (FLOW-miss) (Info) Species: italica (ee-TAL-ih-kuh) (Info) One vendor has this plant for sale. 2 members have or want this plant for trade. Category: Height: Spacing: Hardiness: Sun Exposure: Danger: Bloom Color: Bloom Time: Foliage: Other details: Soil pH requirements: Patent Information: Propagation Methods: Seed Collecting: | Click thumbnail to view: By Joy By ecrane3 By ecrane3 By Joy |
1 positive
No neutrals
No negatives
Gardeners' Notes:
This plant has been said to grow in the following regions:
Dublin, California
Kalama, Washington


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Hey guys, i'm just about to beat the game (i've been invited to the party). I was wondering if i should go look for all the riddles now or if i can go back to the island after i beat the game.
Is there a point of no return?
I was so upset when I found out about 'Party Pooper', watching myself as Batman just pacing down that hallway stone-faced was one of my favorite moments of that game. Knowing that the achievement made people miss out on it bummed me out.
To be fair my experience was probably even cooler and crazier than most because I sniped all the guys outside the doorway with the remoterang so they didn't get to deliver their lines about welcoming me and such, then I saw the rows of thugs inside waiting with my Bat-vision and was super expecting some huge brawl but when the door opened it was like a surprise party.
So I guess the cool part for me was the surprise switcher-oo, and seeing the achievement say "No you should have just fought them" just made me "awww".
I'm a little bummed there isn't a "second quest" option. If I press continued after beating the game it puts me back on the island, but with all of the effects of Titan on the plants. I guess I can still get into all of the old buildings, but it sure feels like there is going to be things closed off.
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text:
Reg Plates Search - RN registrations
RNA RNB RNC RND RNE RNF RNG RNH RNJ RNK RNL RNM RNN RNO RNP RNR RNS RNT RNU RNV RNW RNX RNY RNZ The following reg plates are based on RN number plates
We are currently updating our customer gallery page.
If you have purchased a number plate from us, we would love a picture of your vehicle displaying the reg plate.
You can email to email@example.com, or post to the usual address.
The number plate 1D was bought for a record £352,000 at auction yesterday.
Tycoon Nabil Bishara won it in fierce bidding and plans to put it on his wife's Bentley.
His £352,411 offer beats the previous £254,000 best for a DVLA sale, set by 51NGH in 2006. The DVLA's Damian Lawson said after the auction in Claverdon, Warks: We're absolutely over the moon."
Britain's priciest car reg is F1, bought privately for £440,625 in 2008 by Afzal Kahn of Bradford, West Yorks."


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High water, snow cause chaos on area roads
March 5, 2015 PARKERSBURG — Rain, and not snow, was the cause of problems throughout the area Wednesday morning and afternoon as flood warnings were issued for Parkersburg, S. more »»
Wood offices closed, others open
March 5, 2015 PARKERSBURG - The Wood County Courthouse, Shaver Judicial Annex, Judge Black Courthouse Annex and Justice Center are closed today. The Wood County Sheriff's Office remains open. more »»
Wood Schools closed Friday
March 5, 2015 Wood County Schools will be closed on Friday with employees reporting on Code C, an assistant superintendent said. Employees will report on Code C, Assistant Superintendent Mike Fliing said. more »»
Legislature moves to override veto
March 5, 2015 CHARLESTON — The state Legislature on Wednesday moved to override a governor's veto of a controversial anti-abortion bill. The House voted Wednesday morning to override Gov. more »»
Caution urged on I-77
March 5, 2015 PARKERSBURG – Wood County 911 Director Rick Woodyard urges anyone who does not have to be on the roadways to stay off the streets. more »»
Ashley Flowers waives hearing
March 5, 2015 PARKERSBURG — Ashley Flowers, who is on leave from her job as the Parkersburg finance director, appeared in Wood County Magistrate Court Wednesday for a preliminary hearing on two felony charges. more »»
Senate approves video lottery bill
March 5, 2015 CHARLESTON — The West Virginia Senate has approved increasing the number of terminals allowed in video lottery businesse. more »»
Easter program grows
March 5, 2015 PARKERSBURG — Assuming the snowy results of winter storm Thor have melted away and the Easter Bunny isn't walking around on snowshoes, in less than two weeks spring may have sprung as Parkersburg... more »»
Spring burn season begins
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Police car chase ends in arrest
March 5, 2015 POMEROY, Ohio — Thomas A. more »»
Truancy bill clears Senate committee
March 5, 2015 CHARLESTON — A bill increasing the number of allowable unexcused absences for students in West Virginia before the courts become involved has been sent to the full Senate for consideration. more »»
Senate amends bill blocking Tesla sales
March 5, 2015 CHARLESTON — A bill amending the law dealing with manufacturers and automobile dealers which an electric car maker said will prohibit it from making direct sales to consumers in West Virginia passed... more »»
GOP primary set for Marietta council seat
March 5, 2015 MARIETTA — Seven candidates who have filed, plus any independent candidates yet to file, will ultimately be whittled down to the city's next three Marietta City Council at large members. more »»
Woman arrested for grand larceny
March 5, 2015 VIENNA — A Parkersburg woman was arrested on a charge of grand larceny after allegedly stealing jewelry, officials said. Crystal G. more »»
Russell sentenced on drug charge
March 5, 2015 MARIETTA — A Coolville woman was sentenced to 17 months in prison Wednesday for illegally having a prescription for hydrocodone pills filled under her grandmother's name at a Belpre pharmacy in earl... more »»
Johnson pleads guilty to arson
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Brown pleads guilty in murder-for-hire plot
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Bible College awaiting decision
March 5, 2015 MARIETTA — Nearly two months ago, Marietta Bible College was celebrating after learning that the majority of its student body would not be sent home as a result of immigration issues. more »»
Two students from area in poetry competition
March 5, 2015 PARKERSBURG — Two students from the region will participate in the 2015 West Virginia Poetry Out Loud competition on Friday and Saturday at the Culture Center in Charleston. more »»
Newell complaint being investigated
March 4, 2015 PARKERSBURG — The West Virginia Ethics Commission's Probable Cause Review Board is investigating a complaint against Mayor Bob Newell. The complaint was submitted to the commission Jan. more »»
Attorney in city lawsuit addresses texts
March 4, 2015 PARKERSBURG — The attorney for municipal employees who filed lawsuits against the city related to an alleged affair between Mayor Bob Newell and Finance Director Ashley Flowers addressed the... more »» Trappers event March 6, 2015 GLENVILLE — The West Virginia Trappers Association Spring Rendezvous and Fur Auction will be March 6-8 at the Gilmer County Recreation Center in Glenville.Finished fur will be consigned Friday and Saturday. Raw fur will be consigned Sunday morning and the Fur Auction will be Sunday at 1 p.m. more » Local events |
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Our 2009 Third Grade Summer Reading Picks
Melonhead by Katy Kelly. Silly pranks, sweaty feet, and troublesome tree climbing may not be your idea of story fodder, but they're just the right fuel to get this tale going. Ten-year old Adam Melon is a self-proclaimed expert on famous inventors and the intricacies of toilet paper. When his science teacher announces that every kid in the class will be competing in Challenge America!, an inventing fair, he figures he and his best friend Sam are a shoo-in for first prize. Now they just need to come up with an idea for something explosively cool, without making his mom nervous. A fun read full of pitch-perfect kid humor. (Delacorte Press, 2009, $12.99)
Dessert First by Hallie Durand. It's not always easy being eight. Dessert Schneider finds that out first hand, when her new teacher, Mrs. Howdy Doody, comes into class in her white snowball slippers and urges all her new third graders to learn to march to the beat of their own drummers. For Dessert, that means figuring out her own personal coat of arms, trying to convince her family to eat dessert before dinner, and doing her best to stay out of trouble. If only there weren't so many temptations! Can Dessert resist? Soon-to-be third graders, especially those who've gotten their feet wet with easier chapter books, will enjoy the challenge of reading this confection on their own, while less advanced readers will enjoy it as a read-aloud. Either way, we bet kids will have trouble resisting Dessert! (Atheneum Books, 2009 $14.99)
Wishworks, Inc. by Stephanie Tolan, illustrated by Amy June Bates. To distract himself from the struggles of being the new kid at school, Max imagines a great big dog at his side named King. King is the best dog a kid could want--he's brave, loyal, and intelligent. Unfortunately, he is also highly fictional. When the shopkeeper of a mysterious store called "Wishworks, Inc." guarantees any wish, you can guess what Max wants. But, when a small, ratty dog shows up at the door, he's in for more than he expected. A thoughtful and magical read for kids getting into the groove of chapter books and real-to-life characters. (Arthur A. Levine Books, 2009, $15.99)
Ivy and Bean: Bound to Be Bad by Annie Barrows. Best friends Ivy and Bean are at it again in this fifth paperback installment in the popular series. When the two troublemakers decide that they are going to be so good and pure that wild animals will befriend them, they discover that being good is harder than it seems. Is a little badness a bad thing? Author Annie Barrows' clever and kid-friendly wit, coupled with illustrations that give a kids-eye view of the world, make these books a hit with readers transitioning to chapter books. (Chronicle, 2009, $5.99)
The Sisters Grimm: The Everafter War by Michael Buckley . There's never a dull moment at Ferryport Landing, where Sabrina and Daphne Grimm, descendants of the Brothers Grimm, continue their adventures as "fairy-tale detectives" in this seventh installment of the bestselling series. In a world where fictional characters from fairy tales and nursery rhymes, or "Everafters," are real, it takes everything the sisters have to solve magical mysteries and keep order among the Everafters, even as war is breaking out between Prince Charming's Everafter Army and the dreaded Scarlet Hand. (Amulet Books, 2009, $5.96)
The Lemonade War by Jacqueline Davies. Jessie Treski can do complex math in her head, come up with grownup business plans, and figure out puzzles, but when it comes to understanding people, she's at a loss. So when a letter arrives from school saying that not only will she be skipping third grade, but that she'll be placed in her brother Evan's class, she's deliriously happy, and can't understand why her brother isn't. He declares war...and not just any war, a lemonade war: whoever makes the most money by week's end gets to keep 100% of the winnings. The stakes are high in this chapter book about sibling rivalry, friendship, and fear, and math and money get a fresh, engaging spin. (Sandpiper, 2009, $5.99)


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LAGUNA WOODS - The Friends of the Village invites all residents to come to Clubhouse 5, Thursday, Sept. 17 at 6:30 p.m. and meet the candidates who are running for Third Mutual Board. Because United's last day for filing to be a candidate, comes after the meeting date, Friends of the Village will be unable to interview their candidates.
United will be represented by some experienced United directors who will talk about what a good director must be and then we will open it up to having the whole panel interact.
The candidates running for Third Mutual are: Pat Feeney, Noel Hatch, Carol Moore, Richard Palmer, Lucile Shimon, and Mike Straziuso.
Refreshments will be served.


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Publications (155)719.76 Total impact
- [Show abstract] [Hide abstract]
ABSTRACT: T cells require both primary and costimulatory signals for optimal activation. The primary Ag-specific signal is delivered by engagement of the TCR. The second Ag-independent costimulatory signal is mediated by engagement of the T cell surface costimulatory molecule CD28 with its target cell ligand B7. However, many tumor cells do not express these costimulatory molecules. We previously constructed phage display derived F(AB), G8, and Hyb3, Ab-based receptors with identical specificity but distinct affinities for HLA-A1/MAGE-A1, i.e., "TCR-like" specificity. These chimeric receptors comprised the FcepsilonRI-gamma signaling element. We analyzed whether linking the CD28 costimulation structure to it (gamma + CD28) could affect the levels of MHC-restricted cytolysis and/or cytokine production. Human scFv-G8(POS) T lymphocytes comprising the gamma + CD28 vs the gamma signaling element alone produced substantially more IL-2, TNF-alpha, and IFN-gamma in response to HLA-A1/MAGE-A1(POS) melanoma cells. Also a drastic increase in cytolytic capacity of scFv-G8(POS) T cells, equipped with gamma + CD28 vs the gamma-chain alone was observed.The Journal of Immunology 07/2005; 174(12):7853-8. · 5.36 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Adoptive immunotherapy involving the transfer of autologous tumor or virus-reactive T lymphocytes has demonstrated its effectiveness in the eradication of cancer and virally infected cells. Clinical trails and in vitro studies have focused on CD8+ cytotoxic T-cell receptor (TCR) alphabeta lymphocytes since these cells directly kill virally infected- and tumor cells after antigen-specific recognition via their TCR alphabeta. However, increasing evidence suggests that induction of sustained immunity against cancer and viral infections depends on the presence of tumor- or virus-specific CD4+ T lymphocytes, which are restricted by MHC class II. Here, we show that these MHC class II-restricted CD4+ T lymphocytes can efficiently be redirected to MHC class I-restricted tumor cells by retroviral introduction of an HLA-A1/MAGE-A1-specific chimeric two-chain TCR ValphaCalphazeta/VbetaCbetazeta (tcTCR/zeta). However, TCR-transduced CD4+ T lymphocytes were only able to specifically bind to HLA-A1/MAGE-A1 complexes and respond to HLA-A1+/MAGE-A1+ melanoma cells when the CD8alpha gene was cointroduced. These CD4+/CD8alpha+/TCR(POS) T lymphocytes produce IFN-gamma, TNFalpha and IL-2 when specifically stimulated via the introduced TCR with immobilized HLA-A1/MAGE-A1 complexes or HLA-A1+/MAGE-A1+ melanoma cells. Furthermore, introduction of the CD8alpha gene into TCR(POS) T lymphocytes rendered these T lymphocytes cytotoxic for HLA-A1+/MAGE-A1+ melanoma cells. These results demonstrate that human CD4+ T lymphocytes when genetically grafted with an HLA-A1/MAGE-A1-specific TCR and CD8alpha are induced to kill and produce cytokines upon specific interaction with the relevant melanoma cells. Hence, CD4+ T lymphocytes, in addition to CD8+ T lymphocytes, may be critical effector cells for adoptive immuno-gene therapy to generate a sustained tumor-specific immune response in cancer patients.Gene Therapy 02/2005; 12(2):140-6. · 4.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Tumor-specific receptors may provide effective tools for anti-tumor immunogene therapy. However, the functional analysis of primary human T cells engrafted with tumor-specific receptors is laborious and emphasizes the need for a fast and sensitive method to validate such receptors. To this end, we have set up a Jurkat T cell-based reporter gene assay, and tested receptors with various formats, i.e., receptors based on either a monoclonal antibody (mAb), a full-length T cell receptor (fl-TCR)alphabeta or a chimeric (ch-)TCRalphabeta, and various antigen specificities for their ability to mediate tumor-specific activation of nuclear factor of activated T cells (NFAT). The mAb-based receptor specifically mediates NFAT activation after stimulation with tumor antigen-positive target cells. The observed receptor-mediated NFAT responses were validated by the use of ligand- and receptor-specific mAbs, as well as cyclosporin A (CsA) and a dominant negative mutant of NFAT. Furthermore, anti-TCR mAbs, peptide-loaded tumor cells and antigen-positive tumor cells all resulted in specific NFAT activation in TCR/CD8 co-transduced Jurkat T cells, irrespective of the TCR format used. Importantly, receptor-mediated NFAT responses parallel tumor-specific cytolysis and TNFalpha production of receptor-transduced primary human T lymphocytes. In fact, inhibition of NFAT activation compromises the immune responses of primary human T lymphocytes, pointing to a central involvement of NFAT in anti-tumor T cell responses. Taken together, receptor-mediated activation of NFAT constitutes a representative measure of anti-tumor T cell responses, and the genetically modified Jurkat T cells provide a flexible and sensitive tool with which to select rapidly tumor-specific (chimeric) receptors for immunogene therapy.Journal of Immunological Methods 10/2003; 280(1-2):13-24. · 2.01 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The ultimate goal of immunotherapy of cancer is to make use of the immune system of patients to eliminate malignant cells. Research has mainly focused on the generation of effective antigen specific T-cell responses because of the general belief that T-cell immunity is essential in controlling tumor growth and protection against viral infections. However, the isolation of antigen specific T cells for therapeutic application is a laborious task and it is often impossible to derive autologous tumor specific T cells to be used for adoptive immunotherapy. Therefore, strategies were developed to genetically transfer tumor specific immune receptors into patients T cells. To this end, chimeric receptors were constructed that comprise antibody fragments specific for tumor associated antigens, linked to genes encoding signaling domains of the T-cell receptor (TCR) or Fc receptor. T cells expressing such chimeric antibody receptors recapitulate the immune specific responses mediated by the introduced receptor. Recently, we introduced chimeric TCR genes into primary human T lymphocytes and demonstrated that these T cell transductants acquired the exquisite major histocompatibility complex (MHC) restricted tumor specificity dictated by the introduced TCR. Importantly, the introduction of chimeric TCR bypasses problems associated with the introduction of nonmodified TCR genes, such as pairing of introduced TCR chains with endogenous TCR chains and unstable TCRalpha expression. A novel strategy which is completely independent of available tumor specific T-cell clones for cloning of the TCR genes was recently used to transfer MHC restricted tumor specificity to T cells. Human "TCR-like" Fab fragments obtained by in vitro selection of Fab phages on soluble peptide/MHC complexes were functionally expressed on human T lymphocytes, resulting in MHC restricted, tumor specific lysis and cytokine production. In addition, affinity maturation of the antibody fragment on Fab phages allows improvement of the tumor cell killing capacity of chimeric Fab receptor engrafted T cells. Developments in retroviral transfer technology now enables the generation of large numbers of antigen specific T cells that can be used for adoptive transfer to cancer patients. In this article we summarize the developments in adoptive T cell immunogenetic therapy and discuss the limitations and perspectives to improve this technology toward clinical application.Human Immunology 02/2003; 64(1):56-68. · 2.28 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Repeated administrations of recombinant human interleukin-12 (rHuIL-12) to cancer patients are characterized by a reduction of side effects during treatment. Induction of IFN-gamma, considered a key mediator of antitumor effects of IL-12, is known to decline on repeated administrations. We studied whether other immunological effects of rHuIL-12 are tapered in the course of treatment. In a Phase I study of 26 patients with advanced renal cell cancer, rHuIL-12 was administered s.c. on day 1, followed by 7 days rest and six injections administered over a 2-week time period. Plasma concentrations of various cytokines were monitored, as well as absolute counts of circulating leukocyte and lymphocyte subsets. The first injection of IL-12 was accompanied by rapid, transient, and dose-dependent increments of plasma levels IFN-gamma, tumor necrosis factor-alpha, IL-10, IL-6, IL-8, but not IL-4, as well as rapid, transient, and dose-dependent reductions of lymphocyte, monocyte, and neutrophil counts. The major lymphocyte subsets, i.e., CD4+ and CD8+ T cells, B cells, and natural killer cells, followed this pattern. On repeated rHuIL-12 injections, IL-10 concentrations increased further, whereas the transient increments of IFN-gamma, tumor necrosis factor-alpha, IL-6, and IL-8 concentrations, as well as the fluctuations of the leukocyte subset counts, were tapered. Dose escalation of IL-12 within clinically tolerable margins did not reduce the decline of these immunological effects. Induction of pro-inflammatory cytokines and associated fluctuations in leukocyte subset counts decrease on repeated administrations of rHuIL-12. The steady increment of IL-10 plasma levels may mediate the observed down-regulation of clinical and immunological effects.Clinical Cancer Research 02/2003; 9(1):76-83. · 8.19 Impact Factor - Trends in Immunology 10/2002; 23(9):435-6; author reply 436-7. · 12.03 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: In preparation of a clinical phase I/II study in renal cell carcinoma (RCC) patients, we developed a clinically applicable protocol that meets good clinical practice (GCP) criteria regarding the gene transduction and expansion of primary human T lymphocytes. We previously designed a transgene that encodes a single chain (sc) FvG250 antibody chimeric receptor (ch-Rec), specific for a RCC tumor-associated antigen (TAA), and that genetically programs human T lymphocytes with RCC immune specificity. Here we describe the conditions for activation, gene transduction, and proliferation for primary human T lymphocytes to yield: (a) optimal functional expression of the transgene; (b) ch-Rec-mediated cytokine production, and (c) cytolysis of G250-TAA(POS) RCC by the T-lymphocyte transductants. Moreover, these parameters were tested at clinical scale, i.e., yielding up to 5-10 x 10(9) T-cell transductants, defined as the treatment dose according to our clinical protocol. The following parameters were, for the first time, tested in an interactive way: (1) media compositions for production of virus by the stable PG13 packaging cell; (2) T-lymphocyte activation conditions and reagents (anti-CD3 mAb; anti-CD3+anti-CD28 mAbs; and PHA); (3) kinetics of T-lymphocyte activation prior to gene transduction; (4) (i) T-lymphocyte density, and (ii) volume of virus-containing supernatant per surface unit during gene transduction; and (5) medium composition for T-lymphocyte maintenance (i) in-between gene transduction cycles, and (ii) during in vitro T-lymphocyte expansion. Critical to gene transduction of human T lymphocytes at clinical scale appeared to be the use of the fibronectin fragment CH-296 (Retronectin) as well as Lifecell) X-fold cell culture bags. In order to comply with GCP requirements, we used: (a) bovine serum-free human T-lymphocyte transduction system, i.e., media supplemented with autologous patients' plasma, and (b) a closed cell culture system for all lymphocyte processing. This clinical protocol routinely yields 30-65% scFvG250 ch-Rec(POS) T lymphocytes in both healthy donors and RCC patients.Cancer Gene Therapy 08/2002; 9(7):613-23. · 2.55 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The permanent genetic programming via gene transfer of autologous T cells with cell surface receptors directed toward tumor-related Ags holds great promise for the development of more-specific tumor therapies. In this study we have explored the use of Abs directed to MHC-peptide complexes (or TCR-like Abs) to engraft CTLs with exquisite specificity for cancer cells. First, we affinity matured in vitro a previously selected TCR-like Ab, Fab-G8, which is highly specific for the peptide melanoma-associated Ag-A1 presented by the HLA-A1 molecule. A combination of L chain shuffling, H chain-targeted mutagenesis, and in vitro selection of phage display libraries yielded a Fab-G8 Ab derivative, Fab-Hyb3, with an 18-fold improved affinity yet identical peptide fine specificity. Fab-G8 and Fab-Hyb3 were expressed on primary human T lymphocytes as cell surface-anchored Fab, demonstrating that T cells expressing the high-affinity Fab-Hyb3 molecule eradicate tumor cells much more effectively. Furthermore, the gain in ligand-binding affinity resulted in a 2-log improvement in the detection of peptide/MHC complexes on melanoma-associated Ag-A1 peptide-loaded cells. In summary, an affinity-matured Ab specifically recognizing a cancer-related peptide/MHC complex was generated and used to improve the tumor cell killing capacity of human T cells. This strategy, based on engraftment of T cells with in vitro engineered Abs, is an attractive alternative to the laborious, and in many cases unsuccessful, generation of highly potent tumor-specific T lymphocytes.The Journal of Immunology 08/2002; 169(2):1110-8. · 5.36 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Soluble CD27 (sCD27) reportedly is a sensitive and specific marker for leptomeningeal involvement (LI) of CD27-expressing lymphoproliferations such as B-cell non-Hodgkin's lymphoma (B-NHL) or chronic B-lymphocytic leukemia (B-CLL). Because morphological analysis of cerebrospinal fluid (CSF) in patients suspected of LI is false negative in one-third of patients, a diagnostic marker for LI by B-NHL or B-CLL would be very valuable. sCD27 was determined in the first CSF sample from each of 102 unselected patients submitted for (immuno)morphologic detection of malignant cells. The patients were considered to have LI if either (immuno)morphologic analyses showed tumor cells or if neuroradiological evaluation showed typical abnormalities consistent with LI. Patients were suspected of having LI if CSF samples revealed atypical lymphocytes and/or if clinical symptoms and signs suggestive of LI were present, but clinical follow-up was shorter than 3 months because of deterioration of the patient. LI was considered absent if (immuno)morphologic analyses of CSF samples were negative without evidence for LI during 3 months of clinical follow-up. In patients with chronic lymphoproliferative disorders [mainly B-non-Hodgkin's lymphoma (NHL)], sCD27 concentrations were significantly higher in the CSF samples of 16 patients with confirmed or suspected LI than in those of 46 patients without LI. However, sCD27 was also increased in a variety of other predominantly inflammatory neurological disorders including herpes simplex and zoster infections. The positive predictive value of sCD27 determination for LI was only 54%, but the negative predictive value was 92%. Normal sCD27 concentrations in CSF samples of patients with chronic lymphoproliferation makes LI unlikely, but the determination of CSF sCD27 is not sufficiently specific to serve as a reliable tumor marker.Annals of Hematology 05/2002; 81(4):187-91. · 2.40 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The clinical benefit of adoptive transfer of MHC-restricted cytotoxic T lymphocytes(CTL) for the treatment of cancer is hampered by the low success rate to generate antitumor CTLs. To bypass the need for tumor-specific CTL, we developed a strategy that allows for grafting of human T lymphocytes with MHC-restricted antigen specificity using in vitro selected human Fab fragments fused to the Fc(epsilon)RI-gamma signaling molecule. Retroviral introduction of a Fab-based chimeric receptor specific for MAGE-A1/HLA-A1 into primary human T lymphocytes resulted in binding of relevant peptide/MHC complexes. Transduced T lymphocytes responded to native MAGE-A1/HLA-A1POS target cells by specific cytokine production and cytolysis. Therefore, peptide/MHC-specific Fab fragments represent new alternatives to TCR to confer human T lymphocytes with tumor specificity, which provides a promising rationale for developing immunogene therapies.Gene Therapy 12/2001; 8(21):1601-8. · 4.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: We identified a tumor-associated cytotoxic T lymphocyte (CTL) epitope derived from the widely expressed human MDM2 oncoprotein and were able to bypass self-tolerance to this tumor antigen in HLA-A*0201 (A2.1) transgenic mice and by generating A2.1-negative, allo-A2.1-restricted human T lymphocytes. A broad range of malignant, as opposed to nontransformed cells, were killed by high-avidity transgenic mouse and allogeneic human CTLs specific for the A2.1-presented MDM2 epitope. Whereas the self-A2.1-restricted human T cell repertoire gave rise only to low-avidity CTLs unable to recognize the natural MDM2 peptide, human A2.1+ T lymphocytes were turned into efficient MDM2-specific CTLs upon expression of wild-type and partially humanized high-affinity T cell antigen receptor (TCR) genes derived from the transgenic mice. These results demonstrate that TCR gene transfer can be used to circumvent self-tolerance of autologous T lymphocytes to universal tumor antigens and thus provide the basis for a TCR gene transfer-based broad-spectrum immunotherapy of malignant disease.Nature Immunology 11/2001; 2(10):962-70. · 24.97 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Recovery of cytomegalovirus (CMV)-specific T-cell-mediated immunity after allogeneic hematopoietic stem cell transplantation (SCT) is critical for protection against CMV disease. The study used fluorochrome-conjugated tetrameric complexes of HLA-A2 molecules loaded with the immunodominant NLVPMVATV (NLV) peptide derived from the CMV protein pp65 to quantify A2-NLV-specific CD8+ T cells in partially T-cell-depleted grafts administered to 27 HLA-A*0201+ patients and to monitor recovery of these T cells during the first 12 months after SCT. None of the 9 CMV-seronegative patients became infected with CMV, whereas 14 of 18 CMV-seropositive patients developed CMV antigenemia after SCT. CMV-seropositive recipients of grafts from CMV-seronegative donors required more preemptive treatment with ganciclovir (GCV) than those of grafts from CMV-seropositive donors (3 [1-6] versus 1 [0-3] courses, respectively; P =.009). The number of A2-NLV-specific CD8+ T cells in the grafts correlated inversely with the number of preemptive GCV courses administered (r = -0.61; P =.01). None of the 9 CMV-seronegative patients mounted a CMV-specific immune response as measured by monitoring A2-NLV-specific CD8+ T cells after SCT. Thirteen of 14 CMV-seropositive patients without CMV disease recovered these T cells. In spite of preemptive GCV treatment, CMV disease developed in 4 patients, who all failed to recover A2-NLV-specific CD8+ T cells after SCT (P =.002). Thus, enumeration of HLA-restricted, CMV-specific CD8+ T cells in the grafts and monitoring of these T cells after SCT may constitute a rapid and sensitive tool to identify SCT recipients at risk for developing CMV disease.Blood 10/2001; 98(5):1358-64. · 9.78 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Interleukin 12 (IL-12) has potential efficacy in malignant, infectious and allergic diseases. Its side-effects include activation of coagulation and fibrinolysis, as documented in chimpanzees. We assessed the coagulative and fibrinolytic response in 18 patients with renal cell carcinoma after subcutaneous injection of 0.5 microg/kg recombinant human IL-12. IL-12 induced a fibrinolytic response in 17 patients (94%): plasmin-alpha2-anti-plasmin complexes (PAPc) increased from 11.8 +/- 6.6 nmol/l (mean +/- SD) to a maximum of 18.8 +/- 7.4 nmol/l at 72 h. Baseline levels of tissue plasminogen activator (tPA) and plasminogen-activator inhibitor-I (PAI) were elevated in eight and 14 patients respectively. tPA increased from 12.6 +/- 5.2 ng/ml to a maximum of 19.0 +/- 6.7 ng/ml at 72 h. PAI decreased from 111 +/- 69 ng/ml to a minimum of 65 +/- 53 ng/ml at 8 h, thereafter remaining below baseline. Elevation of PAPc correlated with elevation of tPA and reduction of PAI. A coagulative response occurred in nine patients (50%): thrombin-anti-thrombin III complexes increased from 29 +/- 53 ng/ml to a maximum of 460 +/- 322 ng/ml at 12 h. Patients with and without a coagulative response had similar levels of recombinant human IL-12, interferon-gamma or tumour necrosis factor-alpha. We conclude that IL-12 can activate both fibrinolysis and coagulation in a significant proportion of patients with cancer. The time-frame and sequence of these activation processes differ from those known for other cytokines.British Journal of Haematology 03/2001; 112(2):499-505. · 4.96 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Recombinant T-cell receptors with antibody-like specificity for tumor-associated antigens are successfully used to direct the cytolytic activity of T cells toward tumor cells. Clinical application, however, needs to comply with the low immunogenicity of the recombinant receptor, efficient gene transfer into peripheral blood T cells, and enrichment of receptor-grafted cells. Here, we address these issues and describe an entirely humanized immune receptor for use in adoptive immunotherapy of colorectal carcinoma. The receptor consists of a single-chain antibody (scFv) binding domain specific for carcinoembryonic antigen (CEA), the IgG hinge and CH2/CH3 (Fc) joining region, and the transmembrane and intracellular CD3 zeta signaling chain. To express the receptor in peripheral blood T cells, both GALV envelope and MuLV 4070A pseudotyped retrovirus turned out to be equally efficient, with transduction efficiencies of about 5% to 40%, depending on the lymphocyte donor. Furthermore, receptor-grafted T cells could be 2- to 6-fold enriched by magnetic activated cell sorting, utilizing an antibody directed to the extracellular IgG domain of the receptor. Upon co-culture with CEA(+) tumor cells, receptor-grafted T cells are specifically and efficiently activated to cytolysis and IFN-gamma secretion, demonstrating their feasibility for the adoptive immunotherapy of CEA(+) carcinomas.International Journal of Cancer 11/2000; 88(1):115-20. · 5.01 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Recombinant T-cell receptors with antibody-like specificity for tumor-associated antigens are successfully used to direct the cytolytic activity of T cells toward tumor cells. Clinical application, however, needs to comply with the low immunogenicity of the recombinant receptor, efficient gene transfer into peripheral blood T cells, and enrichment of receptor-grafted cells. Here, we address these issues and describe an entirely humanized immune receptor for use in adoptive immunotherapy of colorectal carcinoma. The receptor consists of a single-chain antibody (scFv) binding domain specific for carcinoembryonic antigen (CEA), the IgG hinge and CH2/CH3 (Fc) joining region, and the transmembrane and intracellular CD3 ζ signaling chain. To express the receptor in peripheral blood T cells, both GALV envelope and MuLV 4070A pseudotyped retrovirus turned out to be equally efficient, with transduction efficiencies of about 5% to 40%, depending on the lymphocyte donor. Furthermore, receptor-grafted T cells could be 2- to 6-fold enriched by magnetic activated cell sorting, utilizing an antibody directed to the extracellular IgG domain of the receptor. Upon co-culture with CEA+ tumor cells, receptor-grafted T cells are specifically and efficiently activated to cytolysis and IFN-γ secretion, demonstrating their feasibility for the adoptive immunotherapy of CEA+ carcinomas. Int. J. Cancer 88:115–120, 2000. © 2000 Wiley-Liss, Inc.International Journal of Cancer 09/2000; 88(1):115 - 120. · 6.20 Impact Factor -
Article: Grafting primary human T lymphocytes with cancer-specific chimeric single chain and two chain TCR.
[Show abstract] [Hide abstract]
ABSTRACT: Primary human activated T lymphocytes were genetically grafted with chimeric T cell receptors (TCR). Three domain single chain (sc-) TCR as well as two chain (tc-) TCR gene constructs were derived from the melanoma-specific cytotoxic human T cell (CTL) clone 82/30, and linked to the CD3-zeta signaling element. Chimeric TCR alpha and beta receptor genes were structurally designed to prevent pairing with endogenous TCR alpha and beta chains in order to prevent the generation of unpredictable immune specificities. After transduction of polyclonally activated human peripheral blood lymphocytes with retroviral vectors harboring the chimeric receptor genes, genetically engineered cells specifically recognized and responded to MAGE-A1POS/HLA-A1POS cells. Importantly, each type of transduced T lymphocytes that bound specifically to peptide/MHC complexes also showed specific antitumor reactivity as well as lymphokine production. Genetically engineered primary human T lymphocytes expressing chimeric sc- or tc-TCR therefore hold promise for disease-specific therapies.Gene Therapy 09/2000; 7(16):1369-77. · 4.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Genetically engineered expression of tumor-specific single chain antibody chimeric receptors (ch-Rec) on human T lymphocytes endow these cells with the parental monoclonal antibody (mAb) dictated tumor specificity and may be useful for clinical immuno-genetherapy. Therefore it was of importance to assess how the densities of tumor-specific receptors and tumor associated antigens (TAA), respectively, affect primary human T lymphocyte functions in relation to target cell susceptibilities to lysis. We therefore studied the functional balance between ch-Rec densities on human T lymphocytes and TAA on tumor cells. The gene construct encoding a ch-Rec derived from (1) a renal carcinoma cell (RCC) specific mouse mAb (G250), and (2) the human signal transducing Fc(epsilon)RI gamma-chain was used. To obtain ch-RecHIGH-POS and ch-RecLOW-POS T lymphocytes, two distinct retroviral vectors were used to introduce the gene constructs into primary human T lymphocytes. Levels of ch-Rec-redirected T lymphocyte mediated tumor cell lysis, as well as lymphokine production were determined using RCC lines as target/stimulator cells, which express either no or increasing densities of the TAA. A functional and dynamic balance between ch-Rec densities on cytotoxic T lymphocytes (CTLs) on the one hand and TAA densities on RCCs on the other, was found. In short, ch-RecHIGH-POS CTLs are triggered by TAAHIGH-POS as well as TAALOW-POS RCCs to lyse tumor cells and produce (IFN-gamma and TNF-alpha) lymphokine. In contrast, ch-RecLOW-POS T lymphocytes are only triggered for cytolysis and lymphokine production by relatively TAAHIGH-POS RCCs. In conclusion, (1) the activation of T lymphocyte responses is co-determined by the expression levels of the ch-Rec on T lymphocytes and the TAA on tumor cells and (2) at relatively high T lymphocyte ch-Rec expression levels the CTLs lyse tumor cells with a wide range of TAA densities. Gene Therapy (2000) 7, 35-42.Gene Therapy 02/2000; 7(1):35-42. · 4.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A phase I study was conducted to characterize the maximum tolerated dose (MTD), dose-limiting toxicity (DLT), and pharmacokinetics of a single dose followed by three times weekly s.c. injections of recombinant human interleukin 12 (rHuIL-12). The study encompassed 28 patients with advanced renal cell carcinoma. rHuIL-12 was administered on day 1, followed by an observation period of 7 days. Starting on day 8, repeated s.c. injections were administered 3 times a week for 2 weeks. The MTD of the initial injection was evaluated at dose levels of 0.1, 0.5, and 1.0 microg/kg. DLT was observed at 1.0 microg/kg and consisted of fever, perivasculitis of the skin, and leukopenia. The MTD of the subsequent repeated injections after 1 week of rest was studied at dose levels 0.5, 1.0, and 1.25 microg/kg. DLT at 1.25 microg/kg comprised deterioration of performance status, fever, vomiting, mental depression, and leukopenia. Other notable toxicities were oral mucositis and elevation of hepatic enzymes. Fever, leukopenia, and elevation of hepatic enzymes were more severe after the initial injection than after repeated injections at the same dose level. At dose level 0.5 microg/kg, the mean area under the plasma concentration-time curve decreased from 7.4 ng/h/ml after the first injection to 3.3 ng x h/ml (P = 0.034) after repeated administrations, and at dose level 1.0 microg/kg, it ranged from 31.8 ng/h/ml to 6.0 ng x h/ml (P = 0.041). One patient had a partial response and seven had stable disease. The MTD of a single s.c. injection of rHuIL-12 was 0.5 microg/kg, and the MTD of three subsequent administrations per week was 1.0 microg/kg. In comparison with a single administration, the three times weekly administrations at the same dose level was accompanied with a milder pattern of side effects and a reduction of the area under the plasma concentration-time curve.Clinical Cancer Research 01/2000; 5(12):3983-9. · 8.19 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: We studied the large-scale production of a variety of natural cytokines during the activation and expansion of human T lymphocytes in a hollow fiber bioreactor culture system. Peripheral blood mononuclear cells (PBMC) were activated using phytohemagglutinin plus recombinant interleukin-2 (IL-2). Phytohemagglutinin was either present in the hollow fiber bioreactor during the entire 15-16-day culture period or only during the 20-h preactivation of the PBMC in culture bags. The expanding T lymphocytes were mainly CD3+,8+ and exerted maximal natural, activated, bispecific monoclonal antibody-redirected and lectin-dependent cytolytic activities between days 9 and 13 of culture. IL-1 and IL-4 were only produced in low amounts. IL-8 and lymphotoxin were primarily produced during the first week of culture. Harvest of the hollow fiber bioreactor culture supernatant at the time of peak cytokine concentration would have yielded per 10(8) PBMC input between 3.7 and 4.9 micrograms of IL-8 (at days 2 or 3), and between 0.02 and 0.5 microgram of lymphotoxin (at days 6 or 7). Tumor necrosis factor-alpha and IL-6 were produced during the entire culture period of 15 or 16 days: per 10(8) PBMC input, between 0.1 and 0.4 microgram of tumor necrosis factor-alpha (at days 2 or 3) and between 0.03 and 0.5 microgram of IL-6 (at days 15 or 16). Production of interferon-gamma and granulocyte-macrophage colony-stimulating factor started from initiation of cultures onwards to reach peak levels at the end of the 15- or 16-day culture period, yielding at that time between 2.1 and 17.7 micrograms/ml of interferon-gamma and between 0.4 and 4.2 micrograms of granulocyte-macrophage colony-stimulating factor per 10(8) PBMC input. The production of tumor necrosis factor-alpha, IL-6, interferon-gamma, and granulocyte-macrophage colony-stimulating factor was proportional to the extent of lymphocyte multiplication. These results demonstrate the usefulness of hollow fiber bioreactor cultures to produce natural cytokines during the activation and expansion of predominantly CD3+,8+ T lymphocytes.Journla of Immunotherapy 08/1999; 22(4):299-307. · 3.35 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Immunophenotyping of haematological malignancies has developed as a clinically valuable but technically complicated diagnostic procedure. It involves a variety of methodological features, in-process strategic judgements and an extensive knowledge of clinical, morphological and other laboratory features of the disease processes under study. We discuss the various internal quality control steps necessary to guarantee reliable results with respect to instrument set-up and calibration; sample preparation; selection and validation of monoclonal antibody panels; and flow cytometric data acquisition, analysis and interpretation of results. The quality of the entire procedure is documented by the analysis of representative specimens in the setting of an external quality assurance programme.Clinical & Laboratory Haematology 07/1999; 21(3):155-60. · 1.11 Impact Factor
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Home > News > Nation
Published: Thursday April 3, 2014 MYT 7:08:00 PM Updated: Thursday April 3, 2014 MYT 7:33:31 PM
by t. avineshwaran
A file photo taken on May 27, 2000 shows Abu Sayyaf gunmen outside a mosque on Jolo Island, Philippines. - AFP
PETALING JAYA: The abduction of two women from the Singamata Reef Resort off Semporna is one of the many kidnapping incidents that have happened in Sabah shores over the years.
In Oct 1979, armed men hijacked a ferry, the Saleha Baru, with 44 people on board off Semporna.
The men released 27 hostages within two days, but 17 were detained for months before they were finally released.
Two passengers were killed in the incident, an 11-year-old girl who drowned and a man who was shot by the hijackers.
In April 23, 2000, an Abu Sayyaf-led group kidnapped 21 people from Pulau Sipadan.
They were taken to their camp in Jolo Island where they were held for six months. They were released in stages with the last released in Sept 11 the same year.
On Sept 12, 2000, 14 men in two speedboats armed with M-16 rifles kidnapped three Malaysians from Pulau Padanan, 38km northeast of Semporna.
The hostages were rescued when Philippine armed forces launched an offensive against the rebels several days later.
On Feb 8, 2010, two Malaysians working at a seaweed farm in Pulau Sebangkat were captured by unidentified gunmen.
The two, Lai Wing Chau, 35, and Tsen Vui Chung, 43, were held captive for more than 10 months before being released on Tawi-Tawi Island in the southern Philippines.
Two years later in Nov 14, 2012, cousins Tung Wee Jie and Wee Wei were kidnapped from their family-run bird's nest farm in Lahad Datu.
Wee Jie, 26, was able to escape from his kidnappers by unshackling himself using a fork after being held captive for almost nine months on Jolo Island.
His cousin, Wee Wei, unfortunately did not survive the ordeal and died while in captivity.
In 2013, two incidents occurred, the first on Aug 27, when about 20 armed men abducted nine Malaysian fishermen from two boats near Mabul Island.
However, the gunmen immediately released the victims and fled after they were tricked by their captives into sailing to a General Operations Force post near Mabul Island.
On Nov 15, armed men raided three of five water villas at Pom Pom Island. During the raid, Taiwanese tourist Hsu Lin Min, 57, was killed, and his wife Chang An Wei, 58 was abducted.
After 36 days, she was released by her abductors and was recovered from the southern Philippine island of Jolo.
Tags / Keywords: Crime, Semporna, Abu Sayyaf, Kidnapping
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Zen and the Art of Justin Bieber Yoga
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Pioneer Editorial: Standards to log on county lands sensible
Government ought not provide onerous barriers in the way of those who wish to make an honest living, but as a steward for the public good, it must also protect the assets in which we have placed our trust in government to oversee for us.
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For new questions please post at magento.stackexchange.com, the community-run support site for the Magento community. We will be providing updates on the new forum solution soon. For questions or concerns please email email@example.com.
I have downloaded the latest version of Magento and tried to install it on a machine running fedora 8, Apache web server, mySQL, etc.
I get to the Configuration option screen and accept all defauls - completing the db access info I leave the checkbox to skp validation of URL's unchecked and it complains that Magento was not found at /localhost:80/magento I select the checkbox to skip validation of URL's and it displays a blank page and I can't get the install to start again.
Not sure what I am doing wrong.
Thanks, Philip


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After almost two years of using DVCS, it seems that one inherent "flaw" is accidental data loss: I have lost code which isn't pushed, and I know other people who have as well.
I can see a few reasons for this: off-site data duplication (ie, "commits have to go to a remote host") is not built in, the repository lives in the same directory as the code and the notion of "hack 'till you've got something to release" is prevalent... But that's beside the point.
I'm curious to know: have you experienced DVCS-related data loss? Or have you been using DVCS without trouble? And, related, apart from "remember to push often", is there anything which can be done to minimize the risk?


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Beauty beyond the beach
Neville Walker leaves the crowded resorts behind and discovers a very different, very dramatic Gran Canaria
It is hard to imagine many of the thousands who now pass through the same airport every day having anything quite so ambitious in mind. Few places are so synonymous with the package tour, and in few places is the visitor's environment so artificial. Purpose-built resorts don't have charming old quarters. And the tour operators' excursions mostly visit attractions that are just as phoney.
But if many visitors to Gran Canaria are hardly intrepid explorers, first impressions as you leave the airport must take some of the blame. Join the motorway and you're confronted with a grim dusty plain. The island's rain is usually anywhere but here, which means the greenery is elsewhere too, up in the mountains. The plastic-covered tomato fields, slummy urbanizacions, and furniture stores don't add much charm. And yes, there is a branch of Ikea.
All this may be a shame for the island's image, but it's great news if you're not so easily put off. It is the paradox of Gran Canaria that, bursting with tourists though it supposedly is, many of its most beautiful and interesting sights can be enjoyed in virtual solitude.
Away from the resorts, the island hasn't been homogenised. A few miles from the McDonald's, Eurosport and English pubs of Playa del Ingles, people in the Barranco de Guayadeque still live in caves.
Guayadeque is one of the most beautiful valleys on the island. It's an easy half-day out from Playa del Ingles. But it's another world. Here, a handful of people echo the lifestyle of the pre-Hispanic Canarians, the Guanches. Sometimes, conventional house fronts are built on to the cave mouths. Halfway up the valley, you can visit a tiny cave chapel at the roadside. At the top, there is even a cave restaurant where you can enjoy a decent Canarian lunch.
Gran Canaria lacks the punch line of a single, central mountain. But it has its own drama: an up-and-down landscape which withholds views until the last minute and then hits you for six with them. In the hills south of Las Palmas, surrounded by posh suburbia, lies the Bandama golf course. If you leave the main road, you drive past with it on your right. A few yards further on, there's nothing but a gaping abyss ahead of you. You're staring into the 600ft-deep volcanic crater of the Caldera de Bandama. It must be the biggest bunker in the world.
You might decide to stop after the long climb from San Nicolas de Tolentino in the west of the island, probably stuck behind an old lorry. Pulling into a lay-by, you might buy an ice-cream, peer over the wall - and gawp at the immense cliff-face of the west coast. The view is completely
unexpected and ends in the Atlantic, hundreds of feet down below.
Sudden vertigo is half the appeal of touring. You can drive from sea level to 6,500ft - way higher than Ben Nevis - in less than half a day. The roads look hairy, but they are well surfaced and, off the motorway, not that busy. Hire a jeep and you can reach some of the most remote and peaceful parts of the interior on unpaved tracks. Only at weekends, when the locals get out and about, is it much busier.
Heading inland from the south coast, the land rises in sudden lurches. With each lurch, the landscape, climate and vegetation change - from bare rock to succulents, to orange groves and date palms, then to orchards and almond blossom. Finally, you reach a pine-clad landscape more like Yosemite than an Atlantic island. It's a surprisingly big country; you begin to understand why westerns were filmed here.
The summit of the island is at Pozo de las Nieves - the snow well. You can drive all the way. The road first allows you a breathtaking glimpse of the north coast. White dots lost in the blue haze above Las Palmas appear to be planes until you realise they are ships out in the Atlantic. The road ends at a car park, over a mile high. From here, the views extend back to the dunes on the coast at Maspalomas, 20 miles away.
Gran Canaria is not just an outpost of Spain. The architecture, fields of bananas and sugar cane, and the volcanic cones and craters everywhere remind you how far you are from Madrid. In fact it feels South America. At carnival in February, the warm streets of Las Palmas fill with crowds dancing to salsa all night, and the European winter seems far off.
Just like the island itself, Las Palmas doesn't appear enticing. It isn't quite the resort it was. Major UK operators don't offer it as an option, and some of the hotels lining the stunning Canteras beach have closed. The motorway along the harbour doesn't help. But what remains is a surprisingly big city with a real metropolitan buzz.
Chief among the attractions is the old colonial area, Vegueta. It's perfect for wandering, with streets and squares of traditional houses, some plain, others baroque. The facades hide balconied patios and lush gardens. Many are public buildings and you can look inside. The cathedral of Santa Ana is certainly worth a peak; baroque on the outside, it is austere, Gothic inside, and has been here almost as long as the Spanish. At its back door is the house Columbus is said to have stayed in.
The biggest surprise in Vegueta is an 18th-century town house in the Calle de los Balcones. Inside it is the Atlantic Centre of Modern Art. Like the Thyssen in Madrid, the classical shell hides an elegant modern space. A venue for temporary exhibitions, including art inspired by the Canaries, if you pick your time you can enjoy it with only the staff for company.
It just shows how easy it is to shake off the crowd when most of the crowd can't be bothered. Exploring Gran Canaria is a doddle. The driving isn't as scary as mainland Spain, the buses are good and you can get the night- bus back if you stay late in Las Palmas. There is not much to avoid: timeshare touts, a few areas of Las Palmas because of crime, and maybe the Kasbah area of Playa del Ingles because it is tacky and sleazy.
A little vertigo, a little sun: no one would expect to be impressed with stirring tales of your intrepid travels in Gran Canaria. It isn't that kind of place; there isn't a Chatwin book in it. But it is worth considering if you're a bit of a misanthrope - or you want more than a beach from your holiday.
There are no direct scheduled flights from the UK. Iberia (tel: 0171-830 0011) flies daily via Madrid. Return fares in August cost from pounds 392 return plus pounds 20 tax. Charter flights start at pounds 200 in August. Flying Colours (tel: 0870 243 0416) does charters to Las Palmas - a return from Gatwick costs pounds 194 including tax in August. Prices are lower early in December and after the New Year.
Magic of Spain (tel: 0181-741 0460) offers holidays away from the main package-tour destinations. A week at the Hotel Santa Catalina in Las Palmas in November costs pounds 679 per person based on two sharing, and including return flights from Gatwick. A week in the scenic north of the island, at the Hotel Santa Brigida, costs pounds 615 per person based on two sharing and including return flights.
Contact the Spanish Tourist Board (tel: 0171-486 8077).
Recommended reading: Landscapes of Gran Canaria (Sunflower Books, pounds 9.99). Mountain Walks in Gran Canaria (Open Air Publishers, pounds 5.95).
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