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Publications (44)154.95 Total impact
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ABSTRACT: Activated leukocyte cell adhesion molecule (ALCAM/CD166) is expressed in a number of malignancies (e.g. prostate, breast, squamous cell carcinoma of the esophagus, lung and head and neck tumors). Based on studies in which ALCAM showed prognostic relevance in several carcinomas, it has been discussed as a potential therapeutic target. We evaluate its expression in head and neck squamous cell carcinomas (HNSCCs). A tissue microarray was constructed from more than 400 HNSCCs. Slides were analyzed by immunohistochemistry for ALCAM. Membranous and cytoplasmic ALCAM positivity were rated separately. The tumors were combined into a) cases with membranous staining and b) cases with cytoplasmic staining, independently from membranous/cytoplasmic co-expression. We found staining in 70.3% of interpretable HNSCCs. Pure membranous staining was found in 12.4% of tumors, with cytoplasmic positivity in 40.1% of cases, and membranous/cytoplasmic co-expression in 17.9%. No significant association between ALCAM positivity and clinical parameters was found. No significant association between ALCAM expression and survival data was observed for all tumors. The frequent expression of ALCAM (70.3%) in head and neck squamous cell carcinomas does not support an important role for HNSCC biology. The increased levels ol ALCAM suggest the existence of a therapeutic window for potential anti-ALCAM therapies.Pathology - Research and Practice 10/2014; · 1.56 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Activated leukocyte cell adhesion molecule (ALCAM) is a membranous cell adhesion protein that is often expressed in breast cancer. Data on the prognostic impact of ALCAM expression is highly controversial in this cancer. To evaluate the clinical impact of ALCAM expression in a sufficiently large patient cohort, we utilized a tissue microarray (TMA) containing more than 2,100 primary breast cancers with clinical follow-up data by immunohistochemistry. TMA spots containing normal breast epithelium showed moderate to strong membranous ALCAM staining. ALCAM staining was strong in 66.2%, moderate in 10.9%, weak in 11.1% and absent in 11.8% of 1,778 (80.9%) interpretable breast cancer tissue spots. Decreased ALCAM expression was significantly associated with advanced tumor size (p=0.0017), unfavorable tumor grade (p<0.0001), negative ER and PR status (p<0.0001 each) as well as high Ki67 labeling index (p<0.0001). Cancers with ACLAM expression loss had a significantly poorer overall (p<0.0001) and disease-specific survival (p=0.0088). This association also held true in the subset of nodal positive cancers (p<0.0001). In conclusion, these data demonstrate that ALCAM is generally expressed in normal and cancerous breast epithelium and that a marked reduction of ALCAM expression characterizes a subset of breast cancer patients with adverse tumor characteristics and unfavorable clinical outcome.Oncology reports. 10/2014; - [Show abstract] [Hide abstract]
ABSTRACT: Cyclin D1 (CCND1) gene amplification is a molecular key alteration in breast cancer and was suggested to predict resistance to antihormonal therapy. As tissue heterogeneity may affect diagnostic accuracy of predictive biomarkers, CCND1 genetic heterogeneity was assessed in this study. A novel tissue microarray (TMA) platform was manufactured for this purpose.Breast Cancer 05/2014; · 1.51 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: NY-ESO-1 has been suggested as therapeutic cancer vaccine in prostate cancer. This study was undertaken to explore the relationship of NY-ESO-1 with tumor phenotype, biochemical recurrence, and molecular subgroups in hormone-naive prostate cancers. NY-ESO-1 immunohistochemistry was analyzed on a tissue microarray containing 11,152 prostate cancer samples. Results were compared to clinically follow-up data, ERG status, and deletions on PTEN, 3p13, 5q21, and 6q15. NY-ESO-1 expression was absent in benign prostate glands. In prostate cancer, NY-ESO-1 positivity was found 8.8% of our 8,761 interpretable tumors including 5.8% with weak, 2.5% with moderate, and 0.5% with strong expression. There was a threefold higher rate of NY-ESO-1 expression in ERG fusion positive tumors than in ERG negative cancers (P < 0.0001). There was a significant association with early PSA recurrence, which was largely limited to ERG positive cancers. Within the ERG positive subgroup, high NY-ESO-1 expression was associated with early biochemical recurrence (P = 0.0002) and high Gleason grade (P < 0.0001). In ERG negative cancers, NY-ESO-1 expression was also linked to PTEN (P = 0.0012) and 6q15 deletions (P = 0.0005). Our observations indicate a tight link of NY-ESO-1 expression to ERG activation and (to a lesser extent) PTEN- and 6q15-deletions in prostate cancer. The impact of these interactions on the likelihood of response to immunotherapy is unclear. The prognostic impact of NY-ESO-1 expression is little and not independent of histologic variables. Prostate © 2014 Wiley Periodicals, Inc.The Prostate 05/2014; · 3.57 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Despite a multitude of p53 immunohistochemistry (IHC) studies, data on the combined effect of nuclear p53 protein accumulation and TP53 genomic inactivation are lacking for prostate cancer. A tissue microarray (TMA) including 11,152 prostate cancer samples was analyzed by p53 IHC and fluorescence in-situ hybridization (FISH). Nuclear p53 accumulation was found in 10.1% of patients including 1.4% with high-level and 8.7% with low-level immunostaining. TP53 sequencing revealed that 17 of 22 (77%) cases with high-level p53 immunostaining, but only 3% (1 of 31) low-level p53 cases carried putative dominant-negative mutations. TP53 deletions occurred in 14.8% of cancers. Both deletions and protein accumulation were linked to unfavorable tumor phenotype and prostate specific antigen (PSA) recurrence (p<0.0001 each). The combination of both methods revealed subgroups with remarkable differences in their clinical course. Tumors with either TP53 deletion (14%) or low-level p53 positivity (8.7%) had identical risks of PSA recurrence, which were markedly higher than in cancers without p53 alterations (p<0.0001). Tumors with both p53 deletion and low-level p53 positivity (1.5%) had a worse prognosis than patients with only one of these alterations (p<0.0001). Tumors with strong p53 immunostaining or homozygous inactivation through deletion of one allele and disrupting translocation involving the second allele had the worst outcome, independent from clinical and pathological parameters. These data demonstrate a differential clinical impact of various TP53 alterations in prostate cancer. Strong p53 immunostaining - most likely accompanying dominant negative or oncogenic p53 mutation - has independent prognostic relevance and may thus represent a clinical useful molecular feature of prostate cancer. © 2014 Wiley Periodicals, Inc.International Journal of Cancer 02/2014; 135(6). · 6.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The Nijmegen breakage syndrome (NBS1) gene was suggested as a prostate cancer susceptibility gene. This study was undertaken to determine, whether NBS1 expression is linked to clinically or molecularly relevant subgroups of prostate cancer. NBS1 expression was analyzed by immunohistochemistry on a tissue microarray containing 11,152 prostate cancer specimens. NBS1 expression was absent or only weakly detectable in benign prostate. In prostate cancers, NBS1 expression was found in 81.3% of interpretable tumors and was considered strong in 41.3% of cases. NBS1 upregulation was tightly linked to ERG positive cancers (p<0.0001). Within ERG negative cancers, strong NBS1 immunostaining was linked to advanced pathological tumor stage, high Gleason grade, and positive nodal status (p<0.0001 each), while high NBS1 immunostaining was only weakly associated with advanced pathological tumor stage in ERG positive cancers (p=0.0099). A comparison with chromosomal deletions revealed a strong NBS1 upregulation in PTEN-deleted cancers, while deletions of 3p13, 5q21 and 6q15 did not affect NBS1 expression. High NBS1 expression was linked to biochemical recurrence in ERG negative and PTEN non-deleted cancers (p<0.0001), which was largely driven by high KPNA2 karyopherin alpha 2 expression. In conclusion, our study identifies an association of NBS1 expression with surrogates of genomic instability in prostate cancer including TMPRSS2-ERG rearrangements and PTEN deletion. The prognostic impact of NBS1 expression in ERG negative, PTEN non-deleted cancers was dependent of the expression status of its interaction partner KPNA2. © 2014 Wiley Periodicals, Inc.International Journal of Cancer 02/2014; 135(6). · 6.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The prognosis of head and neck squamous cell carcinoma (HNSCC) patients is still poor. The identification of high-risk subgroups is needed for the development of custom-tailored therapies. The expression of cancer-testis antigens (CTA) has been linked to a worse prognosis in other cancer types, however, their prognostic value in HNSCC is unclear because only few patients have been examined and data on CTA protein expression are sparse. A tissue microarray consisting of tumor samples from 453 HNSCC patients was evaluated for the expression of CTA proteins using immunohistochemistry. Frequency of expression and the subcellular expression pattern (nuclear, cytoplasmic, or both) was recorded. Protein expression of MAGE-A family CTA, MAGE-C family CTA, and NY-ESO-1 was found in approximately 30%, 7%, and 4% of tumors, respectively. The subcellular expression pattern in particular had a marked impact on the patients' prognosis. Median overall survival (OS) of patients with (1) simultaneous cytoplasmic and nuclear expression compared to (2) either cytoplasmic or nuclear expression and (3) negative patients was 23.0 vs. 109.0 vs. 102.5 months, for pan-MAGE (p<0.0001), 46.6 vs. 50.0 vs. 109.0 for MAGE-A3/A4 (p=0.0074), and 13.3 vs. 50.0 vs. 100.2 months for NY-ESO-1 (p=0.0019). By multivariate analysis these factors were confirmed as independent markers for poor survival. HNSCC patients showing protein expression of MAGE-A family members or NY-ESO-1 represent a subgroup with an extraordinarily poor survival. The development of immunotherapeutic strategies targeting these CTA may, therefore, be a promising approach to improve the outcome of HNSCC patients. © 2014 Wiley Periodicals, Inc.International Journal of Cancer 01/2014; · 6.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Evidence suggests that class III β-tubulin (βIII-tubulin) may represent a prognostic and predictive molecular marker in prostate cancer. βIII-Tubulin expression was determined by IHC in 8179 prostate cancer specimens in a TMA format. Results were compared with tumor phenotype, biochemical recurrence, electroretinographic (ERG) status, and deletions on PTEN, 3p13, 5q21, and 6q15. βIII-Tubulin expression was detectable in 25.6% of 8179 interpretable cancers. High βIII-tubulin expression was strongly associated with both TMPRSS2:ERG rearrangement and ERG expression (P < 0.0001 each). High βIII-tubulin expression was tightly linked to high Gleason grade, advanced pT stage, and early prostate-specific antigen (PSA) recurrence in all cancers (P < 0.0001 each), but also in the subgroups of ERG-negative and ERG-positive cancers. When all tumors were analyzed, the prognostic role of βIII-tubulin expression was independent of Gleason grade, pT stage, pN stage, surgical margin status, and preoperative PSA. Independent prognostic value became even more evident if the analysis was limited to preoperatively available features, such as biopsy specimen Gleason grade, preoperative PSA, cT stage, and βIII-tubulin expression (P < 0.0001 each). βIII-Tubulin expression was associated with PTEN (P < 0.0001) when all tumors were analyzed, but also in the subgroups of ERG-negative and ERG-positive cancers. βIII-Tubulin expression is an independent prognostic parameter. The significant associations with key genomic alterations of prostate cancer, such as TMPRSS2:ERG fusions and PTEN deletions, suggest interactions with several pivotal pathways involved in prostate cancer.American Journal Of Pathology 12/2013; · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Mitochondria are suggested to be important organelles for cancer initiation and promotion. This study was designed to evaluate the prognostic value of MTC02, a marker for mitochondrial content, in prostate cancer. Immunohistochemistry of using an antibody against MTC02 was performed on a tissue microarray (TMA) containing 11,152 prostate cancer specimens. Results were compared to histological phenotype, biochemical recurrence, ERG status and other genomic deletions by using our TMA attached molecular information. Tumor cells showed stronger MTC02 expression than normal prostate epithelium. MTC02 immunostaining was found in 96.5% of 8,412 analyzable prostate cancers, including 15.4% tumors with weak, 34.6% with moderate, and 46.5% with strong expression. MTC02 expression was associated with advanced pathological tumor stage, high Gleason score, nodal metastases (p < 0.0001 each), positive surgical margins (p = 0.0005), and early PSA recurrence (p < 0.0001) if all cancers were jointly analyzed. Tumors harboring ERG fusion showed higher expression levels than those without (p < 0.0001). In ERG negative prostate cancers, strong MTC02 immunostaining was linked to deletions of PTEN, 6q15, 5q21, and early biochemical recurrence (p < 0.0001 each). Moreover, multiple scenarios of multivariate analyses suggested an independent association of MTC02 with prognosis in preoperative settings. Our study demonstrates high-level MTC02 expression in ERG negative prostate cancers harboring deletions of PTEN, 6q15, and 5q21. Additionally, increased MTC02 expression is a strong predictor of poor clinical outcome in ERG negative cancers, highlighting a potentially important role of elevated mitochondrial content for prostate cancer cell biology.Molecular Cancer 11/2013; 12(1):145. · 5.40 Impact FactorThis article is viewable in ResearchGate's enriched format - European Urology Supplements 11/2013; 12(6):128–129. · 3.37 Impact Factor
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ABSTRACT: The extracellular matrix metalloproteinase inducer CD147 has been suggested as a prognostic marker in prostate cancer. CD147 expression was analyzed by immunohistochemistry on a tissue microarray containing 11,152 prostate cancer specimens. Results were compared to tumor phenotype, biochemical recurrence, ERG status and deletions on PTEN, 3p13, 6q15 and 5q21. CD147 expression was strong in benign prostatic glands and often reduced in prostate cancers. CD147 immunostaining was found in 71.7% of 7,628 interpretable cases. CD147 staining was considered strong in 34.6%, moderate in 24.3% and weak in 12.8% of cancers while 28.3% did not show any CD147 reactivity. Reduced CD147 staining was strongly associated with both TMPRSS2-ERG-rearrangement and ERG expression (p<0.0001 each). Within the subgroups of ERG positive and negative cancers, deletions of PTEN, 3p13, 6q15 and 5q21 were unrelated to the CD147 expression status. Decreased CD147 expression was significantly linked to high preoperative PSA values, high Gleason grade, advanced tumor stage (p<0.0001 each), and positive lymph node involvement (p=0.0026) in all cancers. There was a marginal, but statistically significant, association of reduced CD147 expression with early biochemical recurrence (p=0.0296). The significant reduction of CD147 expression in ERG positive prostate cancer provides further evidence for marked biological differences between "fusion type" and "non-fusion type" prostate cancer. Despite a weak association with PSA recurrence, CD147 cannot be considered a relevant prognostic biomarker.Experimental and Molecular Pathology 08/2013; · 2.88 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Increased levels of karyopherin α2 (KPNA2) expression have been described to be linked to poor prognosis in a variety of malignancies. This study was undertaken to evaluate the clinical impact of KPNA2 expression and its association with key genomic alterations in prostate cancers. A tissue microarray containing samples from 11 152 prostate cancers was analyzed for KPNA2 expression by immunohistochemistry. Results were compared with oncological follow-up data and genomic alterations such as TMPRSS2-ERG fusions and deletions of PTEN, 5q21, 6q15 or 3p13. KPNA2 expression was absent or weak in benign prostatic glands and was found to be in weak, moderate or strong intensities in 68.4% of 7964 interpretable prostate cancers. KPNA2 positivity was significantly linked to the presence of ERG rearrangement (P<0.0001). In ERG-negative and -positive prostate cancers, KPNA2 immunostaining was significantly associated with advanced pathological tumor stage (pT3b/pT4), high Gleason grade and early biochemical recurrence (P<0.0001 each). Multivariate analysis including all established prognostic criteria available after surgery revealed that the prognostic role of KPNA2 (P=0.001) was independent of high Gleason grade, advanced pathological tumor stage, high preoperative prostate-specific antigen level and positive surgical margin status (P<0.0001 each). The comparison of KPNA2 expression with deletions of PTEN, 5q21, 6q15 and 3p13 in ERG-positive and -negative cancers revealed a strong link to PTEN deletions in both subgroups (P<0.0001). In conclusion, the strong independent prognostic impact of KPNA2 expression raises the possibility that measurement of KPNA2 expression alone or in combination with other molecular parameters might possibly result in clinically useful information. The data also emphasize a critical role of the functionality of the nuclear import machinery for prostate cancer biology.Modern Pathology advance online publication, 26 July 2013; doi:10.1038/modpathol.2013.127.Modern Pathology 07/2013; · 6.36 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The serine peptidase inhibitor, Kazal type 1 (SPINK1) has been suggested to define an aggressive molecular subtype of ERG-fusion negative prostate cancer. It was the aim of this study to further study the clinical relevance of SPINK1 expression and its relationship with other key genomic alterations of prostate cancer. A tissue microarray containing more than 10,000 prostate cancers with clinical follow-up was used for immunohistochemical SPINK1 analysis. Data on ERG status as well as PTEN, 6q, 5q, and 3p deletions were available for comparison. SPINK1 expression was absent in benign prostate glands and detectable in 5.9% of 9,503 interpretable prostate cancers. Presence of SPINK1 expression was markedly more frequent in ERG negative (10.4%) than in ERG positive cancers (0.3%; P < 0.0001). However, SPINK1 expression was unrelated to tumor phenotype and biochemical recurrence in all cancers and in the subgroup of ERG negative cancers. Further subgroup analyses revealed, however, that-within ERG negative cancers-SPINK1 expression was significantly linked to deletions at 6q15 (P < 0.0001) and 5q21 (P = 0.0042). Our results exclude SPINK1 as a relevant prognostic prostate cancer biomarker. However, the data demonstrate that SPINK1 overexpression is tightly linked to the small subsets of 6q15- and 5q21-deleted ERG negative prostate cancers. These findings support the concept of molecularly defined subtypes of prostate cancers. Prostate © 2013 Wiley Periodicals, Inc.The Prostate 07/2013; · 3.57 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: This study aimed to determine the prevalence and clinical significance of deletions of the tumour suppressor gene PTEN in bladder cancer. A tissue microarray with 686 bladder cancers was analysed for PTEN deletions by fluorescence in-situ hybridization. PTEN mutations were analysed in nine tumours with heterozygous PTEN deletion. Heterozygous PTEN deletions were present in 16.5% of tumours and were associated with grade (P = 0.0024) and p53 status (P = 0.0141), but not linked to stage (P = 0.0965). PTEN deletions were seen in 5.8% of pTaG1, 10.9% of pTaG2, 29.0% of pTaG3, 16.7% of pT1G2, 22.2% of pT1G3, 17.7% of pT2-4G2 and 20.9% of pT2-4G3 tumours (P = 0.0235). PTEN deletions were associated significantly with recurrences in pTa tumours (P = 0.0173), progression in pT1 tumours (P = 0.0016), but not with overall or cancer-specific survival in pT2 tumours. Multivariate analyses including grade and PTEN deletions revealed that PTEN deletions but not grade were associated independently with recurrence in pTa tumours (P = 0.0377) and progression in pT1 tumours (P = 0.0030). No inactivating PTEN mutations were found. PTEN is linked to aggressive tumour phenotype and to unfavourable outcome in early bladder cancer. Heterozygous PTEN loss, i.e. reduced PTEN gene dosage, might be sufficient to cause aggressive tumour behaviour in bladder cancer cells.Histopathology 06/2013; · 3.30 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: INTRODUCTION:: In lung cancer, interleukin-22 (IL-22) expression within primary tissue has been demonstrated, but the frequency and the functional consequence of IL-22 signaling have not been addressed. This study aims at analyzing the cellular effects of IL-22 on lung carcinoma cell lines and the prognostic impact of IL-22 tissue expression in lung cancer patients. METHODS:: Biological effects of IL-22 signaling were investigated in seven lung cancer cell lines by Western blot, flow cytometry, real-time polymerase chain reaction, and proliferation assays. Tumor tissue specimens of two cohorts with a total of 2300 lung cancer patients were tested for IL-22 expression by immunohistochemistry. IL-22 serum concentrations were analyzed in 103 additional patients by enzyme-linked immunosorbent assay. RESULTS:: We found the IL-22 receptor 1 (IL-22-R1) to be expressed in six of seven lung cancer cell lines. However IL-22 signaling was functional in only four cell lines, where IL-22 induced signal transducer activator of transcription 3 phosphorylation and increased cell proliferation. Furthermore, IL-22 induced the expression of antiapoptotic B-cell lymphoma 2, but did not rescue tumor cells from carboplatin-induced apoptosis. Cisplatin-resistant cell lines showed a significant up-regulation of IL-22-R1 along with a stronger proliferative response to IL-22 stimulation. IL-22 was preferentially expressed in small- and large-cell lung carcinoma (58% and 46% of cases, respectively). However, no correlation between IL-22 expression by immunohistochemistry and prognosis was observed. CONCLUSION:: IL-22 is frequently expressed in lung cancer tissue. Enhanced IL-22-R1 expression and signaling in chemotherapy-refractory cell lines are indicative of a protumorigenic function of IL-22 and may contribute to a more aggressive phenotype.Journal of thoracic oncology: official publication of the International Association for the Study of Lung Cancer 06/2013; · 4.55 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) and tissue microarray (TMA) technologies were jointly utilized to search for molecular features associated with clinicopathological parameters in oesophageal cancer. Two TMAs from formalin-fixed tissue samples, including 300 adenocarcinomas and 177 squamous cell carcinomas with clinical follow-up data, were analysed. MALDI-MSI analysis revealed 72 distinct mass per charge (m/z) signals associated with tumour cells, 48 of which were found in squamous cell carcinomas only, and 12 of which were specific for adenocarcinomas. In adenocarcinomas, six signals were linked to early-stage (pT1-T2) tumours (two signals) and the presence (one signal) or absence (three signals) of lymph node metastasis. In squamous cell carcinomas, 24 signals were strongly linked to different phenotypic features, including tumour stage (four signals), histological grade (four signals), and lymph node metastasis (three signals). The high number of m/z signals that were found to be significantly linked to one or more phenotypic features of oesophageal cancer highlights the power of MALDI-MSI in the analysis of high-density TMAs. The data also emphasise substantial biological differences between adenocarcinomas and squamous cell carcinomas.Histopathology 05/2013; · 3.30 Impact Factor -
Article: A novel tool in laryngeal surgery: Preliminary results of the picosecond infrared laser (PIRL).
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ABSTRACT: OBJECTIVES/HYPOTHESIS: Conventional lasers ablate tissue through photothermal, photomechanical, and/or photoionizing effects, which may result in collateral tissue damage. The novel nonionizing picosecond infrared laser (PIRL) selectively energizes tissue water molecules using ultrafast pulses to drive ablation on timescales faster than energy transport to minimize collateral damage to adjacent cells. STUDY DESIGN: Animal cadaver study. METHODS: Cuts in porcine laryngeal epithelium, lamina propria, and cartilage were made using PIRL and carbon dioxide (CO2 ) laser. Lateral damage zones and cutting gaps were histologically compared. RESULTS: The mean widths of epithelial (8.5 μm), subepithelial (10.9 μm), and cartilage damage zones (8.1 μm) were significantly lower for cuts made by PIRL compared with CO2 laser (p < 0.001). Mean cutting gaps in vocal fold (174.7 μm) and epiglottic cartilage (56.3 μm) were significantly narrower for cuts made by PIRL compared with CO2 laser (P < 0.01, P < 0.05). CONCLUSION: PIRL ablation demonstrates superiority over CO2 laser in cutting precision with less collateral tissue damage. LEVEL OF EVIDENCE: N/A. Laryngoscope, 2013.The Laryngoscope 05/2013; 123(11). · 2.03 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Human adenoviruses are known to persist in T-lymphocytes of tonsils, adenoids and intestinal tract. The oncogenic potential of different adenovirus types has been widely studied in rodents, in which adenovirus inoculation can induce multiple tumors such as undifferentiated sarcomas, adenocarcinomas and neuroectodermal tumors. However, the oncogenic potential of this virus has never been proven in human subjects. Using a highly sensitive broad-spectrum qRT-PCR, we have screened a set of different human sarcomas including leiomyosarcoma, liposarcoma and gastro intestinal stroma tumors. Primers binding the viral oncogene E1A and the capsid-coding gene Hexon were used to detect the presence of adenovirus DNA in tumor samples. We found that 18% of the tested leiomyosarcomas and 35% of the liposarcomas were positive for the presence of adenovirus DNA, being species C types the most frequently detected adenoviruses. However, only in one sample of the gastro intestinal stroma tumors the virus DNA could be detected. The occurrence of adenovirus in the tumor sections was confirmed by subsequent fluorescence in-situ-hybridization analysis and co-staining with the transcription factor Bcl11b gives evidence for the presence of the virus in infiltrating T-lymphocytes within the tumors. Together these data underline, for the first time, the persistence of adenovirus in T-lymphocytes infiltrated in muscular and fatty tissue tumor samples. If an impaired immune system leads to the viral persistence and reactivation of the virus is involved in additional diseases needs further investigation.PLoS ONE 05/2013; 8(5):e63646. · 3.53 Impact Factor -
Article: Abundant expression of mTOR kinase in salivary gland tumors - potentials as therapy target?
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ABSTRACT: BACKGROUND: Salivary gland tumors constitute 3-6% of all head and neck neoplasms in adults. Because of limited advances made in the treatment of metastatic disease, the more important is the role of new therapeutic strategies, including molecular therapy. The mammalian target of rapamycin (mTOR) has recently been established as a therapeutic target for several drugs. MATERIAL: Evaluation of phospho-mTOR as a possible therapy target by patients with salivary gland tumors. Immunohistochemical semi-quantitative analyses of the expression of phospho-mTOR(Ser2448) were processed on a tissue microarray containing samples from more than 900 patients. For statistical analysis, contingency table and chi-squared test (likelihood) were used. RESULTS: We observed at least weak phospho-mTOR expression in 25.6-41.2% of all 4 histological adenoma and in 36.8-61.6% of all 11 histological carcinoma subtypes analyzed. No association was seen between phospho-mTOR expression and tumor grade in mucoepidermoid carcinomas. CONCLUSION: In conjunction with literature data providing the evidence for a functional role of mTOR in salivary gland tumors, we conclude that treatment with mTOR-antagonists might potentially also be efficient in wide variety of salivary gland carcinomas.Journal of Oral Pathology and Medicine 03/2013; · 1.87 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: AIB1 (amplified in breast cancer 1) is an estrogen receptorα (ERα) co-activator, known to be amplified and overexpressed in a fraction of breast cancers. It has been linked to prognosis and tamoxifen resistance. However, results have been ambiguous. The different functions of AIB1 in ERα-positive and -negative disease are poorly understood. Therefore, we analyzed the clinical significance of AIB1 in breast cancer with respect to ERα-status and characterized the subgroups. 2,197 breast carcinomas sampled on a pre-existing tissue microarray (TMA) were analyzed for AIB1 expression and amplification by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Results: AIB1 expression was detected in 60 % of the tumors. It was associated with tumor size (p = 0.003), high histological grade (p < 0.0001), poor disease-specific, and overall survival (p = 0.0018 and p = 0.003). There was a strong inverse relationship between AIB1 and ERα expression (p < 0.0001). AIB1 overexpression was associated with increased Ki67 labeling index (p < 0.0001), even if analyzed for different ER expression levels. AIB1 amplification was found in 11 % of the carcinomas. It was associated with high histological grade (p = 0.0012), lymph node involvement (p = 0.0163), and poor disease-specific survival (p = 0.0032) but not with overall survival (p = 0.1672) or ER status (p = 0.4456). If ER-positive tumors were stratified according to their AIB1 amplification status, there was a significant worse disease-specific survival in cases showing AIB1 amplification (p = 0.0017). AIB1 expression is associated with unfavorable prognosis and tumor phenotype. It seems to unfold its oncogenic potential at least in part independent from its role as an ERα co-activator. AIB1 has an impact on cell cycle regulation in ERα-positive as well as ERα-negative tumors. Furthermore, AIB1 amplification characterizes a subgroup of ERα-positive breast cancer with worse outcome. Therefore, AIB1 might be helpful to identify those ERα-positive breast cancers patients who are candidates for adjuvant chemotherapy.Breast Cancer Research and Treatment 01/2013; · 4.47 Impact Factor
Publication Stats
321 | Citations | |
154.95 | Total Impact Points | |
Top Journals
- The Prostate (5)
- International Journal of Cancer (3)
- Histopathology (3)
- Pathology (3)
- Modern Pathology (3)
University Medical Center Hamburg - Eppendorf
- • Department of Pathology
- • Department of Gynecology
Hamburg, Hamburg, Germany
Max Planck Research Department for Structural Dynamics
Hamburg, Hamburg, Germany
University of Hamburg
- Department of Pathology
Hamburg, Hamburg, Germany
Universitätsklinikum Freiburg
- Institute of Pathology
Freiburg, Lower Saxony, Germany


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Set of Three Pre-lit Alpine Trees
Order a set as displayed in the catalog,, the person I spoke with was the best! The shipping arrived in stages, a couple days apart :( had to wait for pieces to arrive individually. Set up was easy, they have been lit for about two weeks the lights are burning out in sections, Not cool..... Slightly over priced
December 24, 2014
Not what I expected
These were flimsier than I thought they would be for the price. The cord is also really short, and they want you to plug directly into the wall. Unless you have an outlet right where you want to plug in, these are really a disappointment. . . .
December 23, 2014
Absolute adorable
Theis tree is one of the prettiest Christmas decor I have recently purchased. I get so many compliments, It warms up an empty corner in my living room. I will be adding more next year!
December 20, 2014
Pining for Better Trees
First, thank you GR for caring enough about your customers to ask for their feedback, I'm sorry to say it's not good.
I have purchased many excellent products from Grandin Road, unfortunately, this is not one of them. I purchased 3 of the 2 foot trees for my mantel display and found them to be of very poor quality. Each tree has a large gap between the shorter top branches and the lower large branches and no amount of repositioning is going to hide the fact. The larger low branches are not going to hold up to any more repositioning, in fact, many flopped back to their original position immediately. When the trees are lit it becomes apparent that little thought was given to the placement of the lights. All of the lights on one tree are on the back side leaving the front top unlit. The "snow" has glued them in place, so the problem has no solution. Luckily trees do not always grow perfectly straight in the forest, so the poor assembly can be chalked up to a natural deformity. Finally, the electrical cord attached is a foot long with a tag attached saying "PLEASE PLUG INTO ELECTRICAL WALL OUTLET ONLY." Unless you want your display on the floor directly in front of a wall socket, you're out of luck.
December 14, 2014
Perfect accent tree
Perfect rustic accent tree for your holiday decor. Moderate flocking (not overdone like a full-blown snow storm) and just enough small white lights to provide a pleasing sparkle. Great for creating groupings of decorations.
December 12, 2014
Not bad
This is the second one I purchased. The first one the lights didn't work after the first year. Disappointed that there are large gaps between the branches. If you don't look too close it looks pretty in a scene with other trees of different sizes in the set of three.
December 10, 2014
Could have been fuller
The tree appears to be fuller in the on-line picture than what was delivered. It kind of looks like a Charlie Brown Christmas tree
December 10, 2014
Quality was poor
I purchased the 4, 5 and 6 foot trees. The 4 foot tree was great. The 5 foot tree's trunk was split in half about 2 feet up, it was then glued together with yellow glue! And how long will that last? Not to mention the look! The 6 foot tree is not straight no matter how I put it together. Was very disappointed in the quality! Might be returning them all!
December 9, 2014
A little disappointed
The two foot trees were a little scarce on branches with big openings on one side I was able to reshape a bit. And place thin area to the back of my mantle. Snow comes off easily. They look nice with some work
December 8, 2014
Love the looks of the trees, but after only being for a short period for 2days, the lights on the 3ft tree went out. I changed the fuse and several of the lights and could not get the string to work. Could not get the string off the tree, so I added another string, which does not have as many lights.
December 5, 2014
prelit alpine trees
The trees were an easy out of box setup. The lights all worked and they look nice in a group or stand alone.
December 5, 2014
2-ft pre-lit Alpine tree
Pretty and well constructed . . . but way too much "snow!" Very messy!
December 3, 2014
alpine christmas tree
We called it our white Charlie brown Christmas tree
and pitched it after one season; enough said.
December 29, 2014
Great purchase
I ordered two sets of these to use on top of buffets in my home. They are absolutely beautiful and were above my expectations. Great purchase.
November 12, 2014
Pre-lit Alpine Trees
I ordered both the 2 and 3 ft Pre-lit Alpine Trees and unfortunately had to send them both back. I've been so happy with my purchases from Grandin Road, however the quality of these trees is not there. There are huge gaps where there are no branches and so much snow applied that the branches are glued together. I've seen the product that was offered last year and it was beautiful! Please bring those back!
December 16, 2014
Short plug-in cords!
I love the 2' and 3' tree. I intended to place them on top of our peninsula fireplace. Unfortunately, the cords are REALLY short and you can't use an extension cord. So, our trees won't light up this year. That's the only complaint I have with them.
December 11, 2014
Just What I Wanted!
I looked at these last year, but waited too late to order and you were out of stock even though it was several weeks before Christmas. As soon as they appeared in the catalog this year I ordered them and they are just what I wanted. I can't wait to decorate!!!!
October 14, 2014
I'm extremely pleased with this tree. It's the perfect size for the top of the bookcase in my office. Can't wait for the holiday season so I can put it up!
October 13, 2014
a great christmas decoration
These were exactly what I wanted for the corner of my room.. I put white snow around the three to hide the wires and it looked great. You have to really work the branches to make them uniform. The only problem I had was on one them the top was a little loose. I can't tell you of durability until I get them out next year
March 13, 2014
LOVE these trees! I bought the 2' and 3' trees. Just took down all my Christmas decorations, but left my trees up on the ledge of my fireplace mantle. They are beautiful!!!
January 2, 2014
Love the trees!
I love these trees! I ordered the 3ft and two 2 ft. Everyone that has been to our house comments on them. They look great!
December 31, 2013
Love the quality
I purchased the pre-lit Alpine trees and unfortunately I could only get two trees of the set of three because the 5ft. trees were sold out. I was disappointed but did however accept the set of two. The 3 ft and the 4 ft.I am very glad I did. I love them and got lots of compliments on them. The delivery time was great. Just a matter of a few days. The quality is excellent and I assure you I will be looking for the Pre-lit Alpine trees next year and will get my order in earlier. I also ordered the long eared owls .I could only get the 6in.All the other sizes were sold out.I will be shopping earlier next year for those as well so I will not be disappointed again. I would recommend these items to anyone who likes to decorate a little different from the traditional .
December 31, 2013
Set of three pre-lit alpines were a little disappointing. Even after fluffing them you could see wide open spaces in the trees.
December 28, 2013
Pre-Lit Decorative Trees
The flocking on these trees makes them alittle messy to work with during setup but one they are arranged how you want them they look nice! We'll see how storage goes with them after the holidays.
December 27, 2013
Unique product
Very happy with the little tree! Only issue is the "snow" falls off very easily.
December 26, 2013
Love them!!
I have them displayed with your pointed ear owls and everyone who has been to my house during the holidays has commented on how beautiful the trees and owls are.
Thank you
December 26, 2013
Apline tree
This tree is simply beautiful! I only wish I had been able to get the 5' one. I put off ordering and they were sold out so i purchased the 4' tree. It is exquisite!
December 26, 2013
Beautiful tree
These little trees are beautiful. Highly flocked, bright lights. A lovely addition to your décor. Highly recommend.
December 25, 2013
So so and odd plug-in requirement
The online item and catalog should clearly state that these trees have to be plugged directly into the wall outlet to work; no such statement is written. The tree does not work with extenstion cords. You don't find this out until you have already received the item and read the paper attached. The tree limbs are a little flimsy as well.
December 19, 2013
Great value!
I bought the 4' and 2 of the 2' trees. They are perfect and the lights really do shine. The smaller 2 are perfect on the mantle. The larger one I have placed in the living room window next to my 3' Santa. The flocking on them is just the right amount for my winter wonderland theme this year. Took only a few minutes to shape and set them up.
Only negative comment is that I would have liked the cord to be a bit longer than it is. I highly recommend them. Great for someone who has a small living space too.
December 17, 2013
(10 Questions : 15 Answers)
Product Q&A
Get help about this item from fellow customers. Ask your questions. Share your answers.
Has staff answer
2-ft. Pre-lit Alpine Tree
1 answer
All the lights have gone out on two of my trees. Since the lights are flocked with the snow, can they be replaced?
If they can be replaced, do you carry the lights? I purchased them in November 2013.
Length of Ownership: < 1 year
2 months, 1 week ago
The 2-ft. Pre-lit Alpine Tree features clear mini lights that can be replaced from a local store that sells Christmas lights.2 months ago
Has staff answer
Set of Three Pre-lit Alpine Trees
1 answer
Does each tree have to be plugged in directly to an electrical outlet?
Set of Three Pre-lit Alpine Trees
I would like to arrange these 3 trees in my foyer where I only have a 2-plugin outlet available. But I read that each tree has to be plugged in directly to an electrical outlet in order to work. Is this correct or can an adapter or power strip be used so that I can plug-in all 3 to the same outlet?
3 months, 1 week ago
Thank you for your interest in the Set of Three Pre-lit Alpine Trees. It is recommended to plug each three into an outlet.3 months ago
3 months, 2 weeks ago
The 3-ft Pre-lit Alpine tree features 50 lights.3 months, 2 weeks ago
Has staff answer
Set of Three Pre-lit Alpine Trees
1 answer
looking for a tree to hang twig ornaments on, will these trees work for ornaments?
Set of Three Pre-lit Alpine Trees
3 months, 2 weeks ago
The Set of Three Pre-lit Alpine Trees will not hold ornaments.3 months, 2 weeks ago
Length of Ownership: < 1 year
4 months ago
Florida, USA
Thank you for your interest in the 4-ft. Pre-lit Alpine Tree. We are verifying the dimensions with our merchants and we will update you as soon as possible.4 months ago
The 4-ft Pre-lit Alpine Tree has a base that is 7" square.3 months, 4 weeks ago
Product:61463-2-ft. Pre-lit Alpine Tree

subset: commoncrawl


text:
Stanford 20/20, 2007/08
Ramnaresh Sarwan
Age: 27 years 202 days
Playing role: Batsman
Batting: Right-hand bat
Bowling: Legbreak
Travis Dowlin
Age: 30 years 321 days
Bowling: Right-arm offbreak
Chris Barnwell
Age: 21 years 5 days
Playing role: Allrounder
Bowling: Right-arm medium-fast
Shiv Chanderpaul
Age: 33 years 148 days
Batting: Left-hand bat
Sewnarine Chattergoon
Age: 26 years 283 days
Derwin Christian
Age: 24 years 247 days
Esuan Crandon
Age: 26 years 25 days
Playing role: Bowler
Bowling: Right-arm fast
Royston Crandon
Age: 24 years 225 days
Lennox Cush
Age: 33 years 30 days
Narsingh Deonarine
Age: 24 years 148 days
Dion Ferrier
Age: 27 years 242 days
Leon Johnson
Age: 20 years 156 days
Neil McGarrell
Age: 35 years 183 days
Bowling: Slow left-arm orthodox
Mahendra Nagamootoo
Age: 32 years 94 days
Bowling: Legbreak googly
New Zealand have more self-belief now
Ricky Ponting: They've drawn from their captain's aggressive style, and their crowds will get in Australia's face. Clarke and Co. have their work cut out
'Where are the allrounders South Africa should be producing?'
Former allrounder Brian McMillan looks back at his career and talks about the country's current World Cup squad
Shami's ODI success, and England's No. 3 problem
Numbers Game: Mohammed Shami has been in excellent ODI form over the last year, but the same can't be said for England's No. 3 batsmen
Playing in the park
The pros make a match of it at the SCG, but all-round entertainment is available elsewhere in Sydney
Throw open the gates to the Associates
Samir Chopra: Keeping them in the World Cup will inspire their countrymen to dream bigger and also prevent the game from being monopolised by a small club
Letter to de Villiers
South Africa's captain needs to single out his players for attention and get them firing individually and as a team
When the MCG was a sea of blue
This was Eden Gardens and another half of an Eden Gardens on top of it. This was Wankhede Stadium with another Wankhede Stadium on top of it
India get their groove back
The long winless Australian summer might just have given those watching the wrong impression
Highest WC score, fastest double-ton, record sixes
Stats highlights from Chris Gayle's record-breaking 215 against Zimbabwe in the Group B match in Canberra
'As soon as you set your fields, the batsman knows your plans'
Former Pakistan offspinner Saqlain Mushtaq on the 1999 World Cup, fighting with Mohammad Yousuf, and hiding his wife in a closet
Letter to de Villiers (276)
India get their groove back (147)
Mask slips along with SA standards (144)
An unusually messy display in the field was followed by more familiar failings during the chase as South Africa felt the heat in Melbourne
Pakistan's troupe of mistakes (88)
His team committed so many errors, one after another, that when Misbah was asked whether the batting, the bowling or the fielding was worse, he just smiled and said "Everything"
Will New Zealand's momentum overpower Australia? (87)
They only need to follow the example of their aggressive captain, who doesn't let insecurity undermine him


text:
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Twitter Party with $680 Travel GiveAwaysJoin us for #MOMtravelchat on Twitter, 10/22 at 9pm EST. The smithsonian will offer advice on seeing its Washington DC museums with kids.… Started by Eileen Gunn |
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Join the conversation to win our daily prize. And, see Friday's winner!
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in Viral Videos
Photo: YouTube
in Music
The show is reportedly set to premiere on E! network in June.
The "Hell's Kitchen" star tests the comedian's palate with a blind taste test.
The Pretenders star is hoping her life story will make people dance and maybe reach for a guitar.
in Music, Viral Videos
What can't she do?
Metallica are feeling retro - so retro, their next release will be on cassette.


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Have you seen the tablets that just came out?
A slow year for tablets finally kicks into gear. Check out the bad boys that debuted this week.
A group of 'Star Trek' superfans is trying to raise money to produce new episodes that are as authentic as possible to the 1960s show.


text:
Activity Feed
g Register or sign in to start receiving activity updates from around Kongregate!
Explore Kongregate & earn 10 kreds Here are your 10 free kreds Explore Kongregate & earn a trophy Here's a shiny trophy for you 15%
Keep exploring Kongregate with more badges and games!
Hide the progress bar forever? Yes No
Topic: Global Assault: General / [Dev] Player Feedback
deathintegra is a prime example of a player abusing the crap out of the team difficulty rating system.
Negative review giving GA one star posted on Google Play store. Don't forget to post your opinion where it counts, folks!
Beat Life or Death after much hammering at it. Got the Galvanizer; opponents are now even HARDER. Don't know why… my team was already full of 4* units, what I'm doing hasn't changed
What remaining interest I have in GA as the leader of guild Panic is dwindling rapidly as my win percentage tanks.
I think I'm about ready to quit. Finally.
BTW folks, it's far from fool-proof, but if you hit info for an opponent on the world map, it will tell you one of their strongest units. Use this in combination with their power icons to help decide if you want to fight them. For instance, I usually pass on any fight where it says they have a Trident right off.
Also, watch for certain power combinations. An opponent with Armor and Strike All icons will likely have multiple Zeus units. Any two together of Cover, Retaliate and Berserk runs a risk of Tridents. And so forth. Watch what fights you take and you can get and idea of what icons might result in what enemy units as you go.
I hope this helps someone until they get the rating system un-botched.
Topic: Global Assault: General / [Dev] Bug/Issue Report
Story mode mission difficulties are out of sync with rewards. If you're going to put me through four-star fights, the reward should be four-star. If the reward is three-star, the fights should be three-star.
Units with no attack power should still "shoot". Why, you ask? Ever have a fight end with just one Hornet on each side?
I have.
Oh, look… ANOTHER Trident casually strolling around the battlefield SLAUGHTERING my forces.
Tridents. Life or Death has TWO. I can only rarely kill ONE on a good day. How does anyone deal with these monsters???
Same goes for Myrmidons. Are you f*cking KIDDING ME?!?
I just lost a fight. For FOUR TURNS STRAIGHT, my Galvanizer and my Auroch Marked ONLY enemy units in back – row positions. But as soon ass I laid my Fireball, my Halefire Humvee and my Icarus in play, the enemy team IMMEDIATELY Marked and destroyed them.
This, both parts, is not uncommon. The AI has exceptional control over what should be random effects, while my guys Mark, Strike and whatnot where it's usually least useful.
Tridents are pretty much insta-win. You can't ignore them and let them hit your base because of Cover. They start with significant attack and defense. Their Berserk is strong. And every time you attack and don't kill them, they Retaliate and so Berserk up.
If a Trident lives to it's second turn, there's a roughly 90% chance it'll start just roaming around decimating my team. By turn 3, it's virtually inevitable.
Any time I see a Trident now, I bail.
Topic: Global Assault: General / Questions
I hate posting from my phone. :P
So I'm still playing, because so far my life makes 2 day researches reasonable, but I have a couple questions:
1) Is it possible to drive down the average level opponent found with sensors? Right now I'M level 33 and I have a team of 3* and 4* free-play units (Zeus, Galvanizer, Icarus, Vile's Incinerator, Halefire Humvee, Auroch, Phoenix x2), and the current batch are kicking my butt because they're either ahead of me in research or they're pay-to-win I'd like to back them up a little because I'm starting to not get dailies done.
2) Do marked units also take extra damage from Strikes? And (I guess three questions) does Armore reduce Strike damage?
Topic: Global Assault: General / Thoughts
I do like the progression of the game, but I probably won't play it for much longer. Here's why:
1) Like just about EVERY OTHER game out there with microtransactions, these guys want roughly between 10 to 100 times as much for their currency as it's worth. 5-14 Volts per buck? Disgusting. This is why I often say microtransactions are the STDs of the video game world.
2) Only one upgrade, one research and one fusion in-process at a time. I understand that this is intended to be a (blatant, completely un-subtle obnoxious money-grab) bottleneck that "encourages" players to buy Volts, but it's just too slow. Which leads me to the third and most obvious reason I'll likely stop playing soon…
3) Upgrade efforts that take DAYS. One day, and soon, I'll click on some research bit that takes 2-4 DAYS to complete… and either I'll just forget about GA completely, or I'll realize that this means any appreciable change in my squad will require WEEKS or MONTHS to complete before I see any further progress…
.. and then I'll be gone.
I've said it before, so many times over the years, and I'll say it again: I'm not against microtransactions, per se, but given that it's effectively money paid for nothing, the prices that are most common in the market are obscene. Everybody just wants way too much.
Other than that, decent game.
Topic: Technical Support / Rotmg account
You can't. The accounts are not transferable, so you'd have to play on their site with an account made on their site.
Topic: Technical Support / Private Kong profiles are known to cause bugs in games?
I was told this by a regular concerning another issue I'd posted here.
Why is this a thing? Are private profiles necessary? How come this hasn't been addressed if Kong insists on keeping them and they're known to cause issues?
Topic: Kongregate Multiplayer Games / [Fantasy Online] Official: FO Help and Support
It's sad that it never occurred to them that the current model of micro-transaction games is broken and maybe they'd have a better time of it if they reduced the cost of in-game goods to 20%-33% of their current prices to encourage spending.
Topic: Cloudstone / The game could use...
When did I say a shield to use with a twohander? I think you're missing the point, crawl.
That makes no sense, crawl. What does the nature of monk claws have to do with giving monks a half decent shield should they choose to use Crystal Dagger (monk-friendly tanking weapon) or Imbued Rapier (for 40s Sword Precision warmonk)?
It's a question related to how the game could use more hybrid-friendly design. Not everybody should have to make (for instance) an EK+Aegis build to progress in the later game.
Topic: Technical Support / Kong API Causing Serious Gameplay Issues in Cloudstone?
That seems like a big problem. Any word on what they're doing to fix it?
On a related note, why are there no decent Discipline shields? There's plenty of Monk-useable one-handed weapons but the only Disc shields are feeble.
It also seems that slower and two-handed weapons are at a disadvantage. They either need to generate more power per hit or their DPS should be greater to make up for the lack of offhand stats and defense (armor/health).
I'm told that the problem is on Kong's end. It's existed for a while. It really, REALLY sucks and is the #1 reason I'm not yet spending money on the game.
In Cloudstone here on Kong, if you add a Kong friend with a private Kong profile, it royally screws up your Allies list. This is a list of characters that belong to Kong players of Cloudstone that your character can summon to help with an area of gameplay and is a key feature of the game.
The bugs vary wildly from player to player. On mine, all my Allies's names come from a select few. There should be only one "Zatoichi", one "clambam", one "scrollwinner" or "hpahpa" or "FredK180"… instead, there are many Allies on my list sharing each of these names, making it a chore to figure what character is actually good to summon for a given task because I have to check their stats instead of just remembering that "halfzue" is awesome. (halfzue appears in my list as one of the several Zatoichi's.)
I've read that, with others, it can lead to bugs as severe as having no access to your Allies list at all! A real gamebreaker!
It seems that this bug has existed for a while. I'm curious as to why it hasn't been resolved yet, and what steps are being taken.
… a little variety. There could definitely be more hybrid gear. In the spirit of Tin Claws (apparently the best hybrid weapon), how about a fury/sorc staff? Or a fury/disc sword?
And perhaps skill mods. A helm that's weak as armor but adds an extra bat to Firebat? Or maybe just +10% to it's damage. Something that boosted Mass Heal would be great for supporting your summons. Finding it on a shield would certainly encourage hybrid play.
And weapons that bend the rules would be fun. A wand that shoots two weaker than normal bullets in a V pattern, or a sword that shoots a wave of energy a short way would change things up a bit.
Topic: Cloudstone / My character build plan so far. Thoughts?
My description of SM is right on the money. It makes my next Healbomb or PK hit harder and cost less, because it restores 5 power when used. If SM is available, I always use it before hitting Healbomb or PK.
I skipped EK because I don't want to count on getting hit any more than necessary to deal my damage, and EK only works when they're successfully landing a hit, which means I must take damage. On the other hand, I can often run around gathering up a mass of smaller creatures, then usually move far enough away for the "bomb" part of healbomb to trigger and kill most incoming monsters before they hit me. It also heals me from the few hits I take in the process of the roundup.
The only reason I'm going hybrid at all is because my current skillset seems to do the things I need it to do (build power, escape if needed, close-range area damage, close range strong-monster extra damage, quick heal, secondary heal, large area attack/long-range attack, summon support), and I have extra skill points coming… but since I'm NOT going hybrid, Fury and Sorcery skills will do me no good, so I feel it'd be better to invest in other class skills that still support a monk. Thus: Grit, Enrage, Toughness, Aegis.
At this point, though, I'm debating whether to keep up with disc+crit gear (since +crit rate also affects skill attacks) or go with tankier disc gear. Also, my current plan still leaves me with 10 skill points unplanned for and I'm debating where to spend them.
Just wanted to review my character skill build plan as a crit-build monk so far and see what folks had to say.
Healbomb 5: I use this both as a quick heal for myself and to deal damage. It's my close range AE attack, where I charge into a swarm of creatures like mudballs and hit it… it makes sure I survive the move AND clears all the small things near me. I have a lot of crit, so it also tends to deal better damage one-on-one.
Claw Mastery 5: I'm using claws to deliver crits more consistently, and because it build Power faster.
Improved Healing 5: to ensure my heals keep me upright longer. More Discipline combines well with this to make for great heals.
Mass Heal 1: My secondary heal, mostly just so I continue to survive but also to help support a Summon. I only put one point in it because more points don't increase the impact of more Discipline, so the better way to boost it is just more Discipline.
Preemptive Karma 1: My ranged attack disc. This combined with a staff is how I attack creatures out of melee range. As with Mass Heal, more points don't improve the Discipline multiplier so more Discipline is the better damage booster. More points WOULD boost the targets hit, but it already hits 8 and if I need to clear a closing mob, I just fall back on Healbomb and THEN PE if I need it.
Haste 5: Increased attack speed helps me dish crits, deal damage and generate Power faster. Additionally, the run speed lets me escape trouble, catch runners or cover long distances quickly.
Spirit Mastery 1: To make that next Healbomb or PE punch harder and cost less.
Bikram Yoga 5: Once I had my action keyset planned out, I started working on passive boosts. Improved Healing makes the impact of this on Healbomb and Mass Heal greater for healing, while Spirit Mastery makes the impact of this on Healbomb and PE a bit more.
I skipped Power Surge because Healbomb is my close range AE damage dealer, and while PS pushes them away, Healbomb heals me. I usually need a heal. I skipped Express Karma for a similar reason… Healbomb being my up-close attack plus heal. I'll chain Healbombs in a longer fight if I want something big dead, but I want them to hit me as little as possible and I want to survive. I have five action abilities (SM, Healbomb, MH, PK, Haste) and keys for jelly juice, speed pots and disc pots, giving me a short boost, a longer boost, two heals, a short range damage dealer and a long range damage dealer in skills.
For my next 16+ points, I was planning on Grit 5, Enrage 5 and Toughness 5 for more passive health and power generation, and then Aegis 1 for a massive survival boost.
But that only takes me to 45. I'm debating what to do with my remaining 10 skill points and WHEN I should spend them WHERE. Many skill require Fury or Sorcery prerequisites, so I can't really use them except as gimp versions because I'm fully focused on Discipline. I could go Aegis 5 for a longer armor superboost. Stretch 5 would give me 15 more Disc, which impact would be boosted by IH for heals and SM for damage as before. The benefit per skill point of both is low, though… 3 Disc per point or 1 second of Aegis per point past the first. Or I could go Staff Mastery 5 (because I occasionally use a staff for ranged fighting), and then Ponderings 5 for +50 power… but I rarely use staves and have plenty of Power generation already (fast weapon, haste, spirit mastery, enrage).
Input would be helpful!


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Learn more about our degree programs and admission requirements
Find the information you need to conduct your research and academic studies
See KCUMB's beautiful campus
See faculty research interests
See five-year campus master plan
Interested in listing a classified? Email Patrick Allen or call 816-654-7287 today! Check out KCUMB employment opportunities.


text:
Doubles With You : Quaisoir & Jeabzz
18 10 Share TweetTake a look at a unique collaboration between jeabzz (France) and quaisoir (Singapore) - it's a double weddings collaboration!
Hi everybody :) Let me present to you my collaboration with quaisoir! The only doubles I've already made were with friends living not really far from me…(lakayou and rmnooii, living in Paris and Berlin…) So when quaisoir explained to me that she was living in Singapore, I thought it was the perfect occasion to mix my shots with something totally different!
I shot a roll with my Lomo LC-A, rewound it, bid my farewell, "Bye bye dear little roll" and sent it to her in the other side of the world…
She used the roll during a wedding in Singapore. We were really happy with the results and after that, she decided to send me a second roll that she shot in Bali, during her holidays. I received the roll just before a friend's wedding in France and i decided to use it at my friend's wedding … and so it was the double "weddings" collab between Paris and Singapore! :)
I really enjoyed this collaboration with quaisoir, and I hope to do some more like this! I hope you will like it and enjoy :)


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PORT CHARLOTTE — The Tampa Bay Rays have a new look and the same old plan for success.
Pitching and defense propelled the budget-minded club during a stretch of six consecutive winning seasons that produced four playoff berths, two AL East championships and one trip to the World Series.
And president of baseball operations Matt Silverman sees no reason why the Rays can't continue to thrive after a winter of dramatic change.
Larry Bugg
The sport of high school boys weightlifting probably began when football coaches realized a little competition might draw some players into heavy lifting and the results could be boys lifting for the fun of it.
Some boys could earn medals and build up weight and muscle for football and other sports.
C.J. Risak
It seemed likely that Seven Rivers Christian would be ready for a big softball season. After all, the Warriors were 14-4 going into last year's 2A tournament, and everyone from that team was returning.
Check out the numbers posted by Seven Rivers Christian's Delaney Byers and it would be impossible not to be impressed. Still, there's one figure that stands out.
Her age. She's just a freshman.
HOMOSASSA — A walk is as good as a hit, at least the saying goes.
For Seven Rivers Christian's Ocala rival, a three-walk inning and a Warrior error were good enough to overcome a no-hit complete game by Warrior freshman Parker Pillsbury on Monday.
Tony Castro
Imagine a world where prep athletes leap by the season from sport-to-sport-to-sport: like in years gone by.
Attempt to ponder why many of today's millennials stick to one sport, rather than dabbling in many.
Florida won't be penalized for former assistant football coach Joker Phillips having impermissible contact with a recruit.
The NCAA released its ruling Friday, saying the Gators handled the situation appropriately by taking corrective action and self-imposing penalties. A Division I Committee on Infractions panel assigned no additional penalties or measures.
Former Lecanto High School wrestler Christopher Michael McWherter was termed by two-time state Panther placer Greg Meade as "the best Lecanto wrestler to never make states."
To this day, the 34-year-old McWherter isn't brushing off the label as untrue.
INVERNESS — Visiting Lecanto squared off at Citrus on Friday night in the final regular season action between the Class 2A District 5 foes during the Hurricanes' Senior Night festivities.
CHS honored three members of its Class of 2015: Devon Carmack at 126 pounds, Victor Segarra at 145 and Johnathan Loggins at 285.
CRYSTAL RIVER — There's a score that most, if not all, of Seven Rivers Christian's softball players remember, although they probably wish they didn't.


text: Latemodelrestoration.com offers Mustang running pony decals so you can proudly display your love for the breed. These Mustang Running Horse Decals come in wide variety of sizes and colors as well as both left hand and right hand specific decals. Whatever style suites your needs, LRS has got all the Mustang Running Horse Decals you could want. Looks great on any 1994, 1995, 1996, 1997, 1998, 1999, 2000, 2001, 2002, 2003, and 2004 Mustangs.


text:
USI RV Park
Phone: (316) 838-8699
Visit Campground Website
Sites: 79
Recent Rate: $40 10/2014
Latitude: 37.7451322114863
Longitude: -97.3020780086517
These reviews are the opinion of an RVParkReviews' member and not the views of RVParkReviews.com
Date of Stay: October, 2014 - $40.00
Nice park on the North side of Wichita - easy access from Hwys.135 & 96. Gravel roads, and long, roomy pull-through sites, with some shade trees. Escort to site; friendly staff. Large "pet walk" with grass across from back row, Row D - great for our two Labs. If visiting Wichita, we will stay here again. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: October, 2014 - $36.00
Extremely friendly staff. The sewer drains were a stretch to get to. The biggest issue I have with this park is it is just a huge gravel parking lot. There is no green space to speak of. There was a 'Good Sam' discount, but the rate still seemed a little high. The little green area there is, is off limits to pets. Makes it difficult for them to use the bathroom. When we stayed we were warned that there is one camper that is very diligent about watching for dogs. He turned other campers in because they left their dogs tied up 'unattended' for 7 mins. If you need to get 'plugged in' to recharge after a few days of dry camping, have no dogs and don't want to sit your lawn chairs/ picnic table on grass, it's ok. Did have a few issues with the 50 amp connection. But it has been working since then. We camped at USI RV Park in a Motorhome.
Date of Stay: June, 2014 - $40.00
A little pricey even with Good Sam discount but the gravel sites were level. Staff friendly. Strouds Restaurant within walking distance very good - pan fried chicken. Cable TV OK. Wi-Fi free but WEAK! Would stay here again. We camped at USI RV Park in a Motorhome.
Date of Stay: August, 2014 - $45.00
For an overnight stop, or for staying in the Wichita area this is a really nice campground. It's not a destination park, but if you're just needing a place to stay it is great! Quiet but convenient to the highway. Very secure. The grounds are clean and well maintained with grassy areas around the periphery of the camping area. Playground equipment is new and nice for the little ones. Large grassy area for pet walking. And quite a bit of shade. The owners/employees are super friendly and helpful. We were very impressed. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: May, 2014 - $40.00
A very nice RV park. The restrooms and showers were very clean, and the park hosts were super friendly and helpful. Good dog walking area and they were building a dog park area while we were there. Plan to stay there again next time we are in Wichita. We camped at USI RV Park in a Motorhome.
Date of Stay: September, 2013 - $50.00
The park is easy to get to & from Interstate 135 using HWY 96. The GPS took us from I-135 to HWY 254 onto local roads. Wouldn't recommend that route. The interior roads and sites are gravel. The roads were not very wide and the turn radius were somewhat tight for larger RV's. Most all of the sites were pull through but not necessarily long enough for larger coaches with their toads attached. There were only a few back ins. The sites were level as the park was built on flat ground. The sites were somewhat close to each other. They have full hook ups – water, electricity (50 AMP), & sewer connections. The connections were centrally located along the sites but slightly elevated so sewer flow could be an issue. There was an outside table. There were a lot of trees throughout the park so sufficient space to open slides could be an issue. Due to the amount of trees in the area, a clear view of the southern sky to get satellite reception could be an issue. We specifically asked for a satellite accessible site. They didn't offer cable and there were only a few broadcast stations available. Their WiFi reception was good. There seem to be a lot extended stay residents everywhere in the park with a lot of clutter and encroachment on the roads. Although their map and facilities list included a fenced dog area, no such area existed. The designated pet area was a large grassy field with a waste collection station. The only other option to walk the dogs was interior roads. Not a very good option. The trash dumpsters were conveniently located to our site. They might have been a walk from other sites. We only chose this park for one night stay because of the negative comments posted about the other parks in the area. For the cost of this park, it didn't meet up to expectations. If traveling through the area again and needed a place to stay for the night, we would NOT return. Should have picked one of the other parks. We paid $45.00 with our Good Sam discount. We camped at USI RV Park in a Motorhome.
Date of Stay: September, 2013 - $44.00
Very nice park. Clean and level sites, but they are close together. Not a place you would go camping, but easy to stop for the night if heading south or north. Great customer service. Very nice staff. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: August, 2013 - $325.00
What a wonderful park! Staff is helpful, friendly, and eager to help with everything! Very family oriented! Great new playground equipment, lots of space for kids to play. Large pet area. Nice, clean shower houses. On-site laundry facility and propane. We have been here since March and LOVE this place!!! We camped at USI RV Park in a Fifth Wheel.
Date of Stay: August, 2013 - $325.00
Great park - brand new playground. Great trees. The friendly workers in all of Kansas. The place is always clean, the restrooms are spotless. Huge pet area and close to everything. Very kid friendly, open areas for them to run and play, and lots of other kids to play with. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: August, 2013 - $35.00
When we got here, there were campers and trucks everywhere. Places are tight: hardly room to even park, much less sit outside and cook a hamburger. Lots of people living here, people walking through park with 3 or 4 beers. Camper next to us got in domestic fight. Campers are way too close together. We wanted to leave early and were refused a refund. They even charged us $8.00 a night for our 2 grandchildren to stay, and then they never stayed. Would not even refund that. The only good thing was the nice play ground equipment, but even with a no dog sign up, there was dog Waste. Never again and we visit Wichita often. We left and stayed in a motel, left the camper, but received NO refund. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: April, 2013 - $30.00
Very friendly staff and a person escorts you to your site. All gravel and not necessarily level. Many permanent residents. A very clean park. Strong Wi-Fi. Located in an industrial area. Very easy to locate with GPS. We camped at USI RV Park in a Motorhome.
Date of Stay: August, 2012 - $44.00
Good campground, but sites are rather short and tow vehicles must be parked perpendicular to rigs, thus extending into driving lanes. Sometimes, it is a little tight to drive through. Also, can be tight entering or exiting a site if a vehicle is in the way. Watch tree branches overhead--most are fine, but some can be risky if you are not careful. New bathrooms and showers are very, very clean. In spite of the tight spots which are manageable, we recommend this campground. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: May, 2012 - $35.00
Staff was excellent. They were very helpful with directions both to the RV Park and to our destinations in the Wichita area. Staff assisted us with parking. Restroom and showers were spotless. As this was a holiday weekend and our reservations were made a couple of days earlier, we were parked in the overflow area. Not a problem. Site was very quiet, safe, and easily accessible. Appeared that there were many long term occupants, but the sites were all neatly kept. Very convenient to Wichita area. I would recommend this RV park - but would suggest making reservations as far ahead as possible. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: June, 2012 - $32.00
Easy access from highway, the drives and lots are gravel. Some shaded lots, railroad close by so you will hear trains early in the morning (5:00 AM). Playground equipment is in much need of repair. The park is OK if you are just passing through. We camped at USI RV Park in a Fifth Wheel.
Date of Stay: June, 2012 - $32.00
Seems friendly, drives and lots are gravel and some sites have shade. We camped at USI RV Park in a Fifth Wheel.
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Very nice owner, room was functional, clean, and comfortable. Had micro fridge. Bed was nice. Suited my needs well.
too close to the highway, heard traffic all night. Moldy grout in bathroom.
Very nice, well equipped suite. This had separate bedroom and living room areas. The bed was comfortable. Breakfast was good, with sausage patties & make-your-own waffles. Staff was very friendly.
Fruit was not well stocked & ran out before we got any, even though the hotel was not very busy. They did not offer any eggs for breakfast.
Beautiful room, beautiful view, clean, friendly & helpful staff, easy to find location, well decorated & interesting public places. It was like staying in a museum. Great & easy to find location.
The hotel was decorated with pictures and displays about the local history and culture.
The breakfast offered was below par. There was no meat, not even in the gravy.
Bed was comfortable
It needs extra towels in room and extra blanket.
Everything. The room was in bad condition. Ceiling being replaced in bathroom marks as scuffs everywhere. The refrigerator was going out and made a ticking noise the entire time. The tv was crappy. The outside of the hotel was is bad shape.
room ceiling is highspa and indoor pool are really nice
the air conditioner was too loud
Tammy at the front desk was very friendly and helpful. She told me about IHS and signed us up. We have stayed at 4 Holiday Inn Express (and 4 other hotels) in the past 3 months, but was never told about this program until we met Tammy
Great stay... nice size room... fairly new hotel... has a fitness room, very clean ...friendly staff in a good location.
No hot tub or inside pool.
The room was Clean and quiet. The breakfast had a lot of choices and was was very good.
Clean room, comfortable room and size
decor was a little odd in the rooms, but the historic posters and documents on the hallways were great
Rooms were very spacious, clean, and comfortable. Great breakfast. Very friendly staff.
You have to drive a little to find food. Pizza will not deliver. That is not the hotels fault.
The breakfast was better than the average and the water pressure was awesome.
The woman at front desk didn't look closely at my reservation to see that I had already paid for the room through another website and charged me again...working on getting it fixed. The room was slightly dated (tv, furniture,decor)
The staff was friendly and helpful. Breakfast was very good. A very nice place to stay.
place to sleep
door jam busted unable to secure door with dead bolt unsacure parking area. would not stay there again.....
did not liked, there was mold on the wall behind the bed!
they over charged even though we confirmed the price on price line!
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The Test championship mace is out of their reach but a plump prize purse of USD 750,000 is at stake when India take on Australia and England clash with New Zealand in their respective series in the days ahead.
South Africa are assured of top place and a prize money of USD 450,000 at the April 1st cut-off date but a further USD 750,000 will be distributed among the teams finishing second (USD 350,000), third (USD 250,000)and fourth (USD 150,000). Australia are currently ranked third and lead fifth-ranked India by 12 ratings points. However, this could potentially change, depending on how the series pans out.
India can move ahead of Pakistan and finish in fourth position if it wins the series by 1-0 or better. To move ahead of Australia, India will lead to win all the four Tests of the series. To be guaranteed second position on the table, Australia need to win the series 2-0 or better. If Australia wins 2-0 and England beats New Zealand 3-0, then both Australia and England will be locked at 119 ratings points but Australia will be ranked above England when the ratings are calculated beyond the decimal point.
Players' rankings
Australia captain Michael Clarke is occupying second position, six ratings points behind South Africa's Hashim Amla, while India's Sachin Tendulkar is in 20th spot. In the Player Rankings for Test bowlers, Australia's Peter Siddle is the highest-ranked bowler in fifth position, with fellow paceman Mitchell Johnson in 18th. For India left-arm spinner Pragyan Ojha is in eighth spot and his fellow spinner Ravichandran Ashwin is in 20th place.


text: Welcome to our listings for Audio Systems 30W - 50W CD Player . We aim to provide a comprehensive shopping resource for our users, bringing together all the information you need to buy the the product that's right for you at the best possible price. You can choose to sort our listings by price ... View more


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POPSUGAR Celebrity 2009 Espy Awards Photos of the Espy Awards in LA by Celebrity 7/16/09 0 Shares Like us on Facebook Previous Photo 1 of 81 View on One Page ADVERTISEMENT ( more slides) Next Share This Link Copy 1 Photos of the Espy Awards in LA Previous Photo 1 of 81 View on One Page ADVERTISEMENT ( more slides) Next Read More 2009 Espy AwardsRed Carpet Red Carpet Stars of Disney's Cinderella Have a Ball at Their Enchanting Premiere This May Be Gigi's Prettiest Runway Moment Ever 21 Reasons Lily James Is Fashion's Newest It Girl Stunning Oscars Pictures You Haven't Seen Yet Get the scoop with our daily newsletter Enjoy the latest news on the celebs you love. A Listers Young Hollywood TV Movies Sign up with 5 years The girl in the white dress in Victoria's Secret supermodel and Orlando Bloom's girlfriend Miranda Kerr. Yogaforlife 5 years Don't know who the girl in the white dress is, but could she have the exact same pose in every picture? Link Love Sara Gilbert welcomes a baby boy with Linda Perry — Us Weekly Kendall Jenner's bikini pic will make you yearn for Summer — HuffPost Celebrity Find out where Brad Pitt's career is going next — Lainey Gossip Why Cameron Diaz isn't into social media — Dlisted Kelly Clarkson: "I don't care if my children are gay" — ET James Franco explains his creative process — JustJared Bella Thorne kicks off her week with a new look — Hollywood Tuna Here's how Kanye West spent his weekend — Pink Is the New Blog About that "unretouched" photo of Cindy Crawford — The Superficial Charli XCX's new outfit will make you do a double take — The Blemish Latest Celebrity
Bethenny Frankel, Ramona Singer Sob Over Their Divorces in Real Housewives of New York City Season 7 Trailer: Plus, Meet New Cas Us Weekly 25.4K Shares


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season 8 tweet leeks starting
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Join Date: Jun 2012
Posts: 45
# 83
And Kahless came down from the mountain, and carried with him two stone tablets, upon written were the 10 KDF commandments:
Originally Posted by
We want a complete KDF faction!
1.-We want Klingon patrol missions in our systems and visit planets like Feds can Andoria, Vulcan, Risa etc.
2.-We want Klingon FE & story related content that is not just copied from the Feds even if it's not level 1-20 content but end game FEs.
3.-We want more uniform options!
4.-We want Lethean & Ferasan ships as well as a few more T5 from the other factions.
5.-We want more bridge options for the KDF factions that are not Klingon.
6..-We want KDF exploration/nebula/cluster missions that are more than just blow that, kill 'em, step on the tribble...
7.-We want the affiliated races in the KDF to be more represented in Klingon content
8.-We want another Klingon sector block with one more station/social zone.
For the part of this that goes into C-Store, I'm willing to pay as I supose many KDF dedicated players are!
10.-We want the KDF bugs that break Klingon gameplay fixed rather than ignored.
Nothing bad intended, just telling them WE want it so it doesn't fall off their radar!
So let it be written, so let it be done.


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Lol, you'll pick one eventually. xD
Doesn't mean it has to be trouble! xD
Hahah, well thats not good. xD
I don't know whether to see that as kinky... or troubling.
No worries bab, at all
Haha no worries. You don't need to ask if you have ideas bab. I actively encourage you just throw them out- whether that be in the Pit, by PM or on Skype. Hell, we've had discussions by video for this stuff!
Go for it!
Trying to lock me up?!
Trying to be a fed?
Film Analysis. I chose to do mine on 'Black Death' a film centering around the Black Plague. Had to determine whether or not it accurately protrayed the historical event(in my case, the Bubonic Plague)
The Trinity
Black Ninja!
Normal Member
© 2011 eWrestlingNews, All Rights Reserved.


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#41667810 -
night ferris wheel
© Yury Zap - See portfolioPricing
Author | Yury ZapSee portfolio |
Image reference | 41667810 |
Country | Japan |
Descriptive Category | Architecture Infrastructure Theme Park |
Conceptual | Travel Asian Landmarks Tokyo |
Keywords |
amusement architecture bench big bright city ferris green huge illumination lamp light modern night park pink red scenic sky wheel
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Vermont Calendar of Events
05/17/12 - 05/17/12Montpelier
Mother Nature's Child Free Showing & DiscussionCity: Montpelier
Place: Kellogg-Hubbard Library
Price: Free
Time: 6:00pm
Contact Name: Caroline Simpson
Phone: 802-223-3338
Email: firstname.lastname@example.com
Come view this inspiring film showing children interacting with nature TODAY and the groups and teachers who make it possible. Afterward, join in our discussion of the movie. The Kellogg Hubbard Library along with Transition Town Montpelier & The Nature Conservancy Sponsor
Tweets by @Vermontcom


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Jump to: Article | Report or Working Paper Number of items: 2. ArticleGray, S., Goodyear, H. and Jones, M. (2005) Outcomes of flexible training compared to full time training during the Specialist Registrar Grade in the UK. Medical Education Online, 10. pp. 1-2. ISSN 1087-2981 Report or Working PaperWilliams, A., Merrell, J., Bodycombe-James, M., Jones, M., Davies, S. and Eaton, N. (2002) Evaluation of Caerphilly Sure Start and Children and Youth Partnership Programmes. External Report. UNSPECIFIED. This list was generated on Sun Mar 1 02:56:40 2015 GMT. |


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Notes on prices
The more than twenty contributions in this book, all new and previously unpublished, provide an up-to-date survey of contemporary research on computational modeling of the visual system. The approaches represented range from neurophysiology to psychophysics, and from retinal function to the analysis of visual cues to motion, color, texture, and depth. The contributions are linked thematically by a consistent consideration of the links between empirical data and computational models in the study of visual function.An introductory chapter by Edward Adelson and James Bergen gives a new and elegant formalization of the elements of early vision. Subsequent sections treat receptors and sampling, models of neural function, detection and discrimination, color and shading, motion and texture, and 3D shape. Each section is introduced by a brief topical review and summary.Michael S. Landy is Associate Professor of Psychology at New York University where J. Anthony Movshon is Professor of Neural Science and Psychology and Director of the Center for Neural Science.Contributors: Edward H. Adelson. Albert J. Ahumada, Jr., James R. Bergen. David G. Birch. David H. Brainard. Heinrich H. Bülthoff. Charles Chubb. Nancy J. Coletta. Michael D'Zmura. John P. Frisby. Norma Graham. Norberto M. Grzywacz. P. William Haake. Michael J. Hawken. David J. Heeger. Donald C. Hood. Elizabeth B. Johnston. Daniel Kersten. Michael S. Landy. Peter Lennie. J. Stephen Mansfield. J. Anthony Movshon. Jacob Nachmias. Andrew J. Parker. Denis G. Pelli. Stephen B. Pollard. R. Clay Reid. Robert Shapley. Carlo L. M. Tiana. Brian A. Wandell. Andrew B. Watson. David R. Williams. Hugh R. Wilson. Yuede. Yang. Alan L. Yuille.
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from Poseidon (2006)
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Jennifer Ramsey: [walks to poker table with Christian] We're going downstairs now.
Robert Ramsey: Ok, have fun... Hey Jen...
Jennifer Ramsey: [looks down at her cleavage] The twins? Dad...
Robert Ramsey: Just one more button.
Jennifer Ramsey: [closes a button of her shirt] Pair of 5's, huh dad?
[Jennifer walks away with Christian]
Dylan Johns: [about his poker game] All in.
Robert Ramsey: [condescendingly] I fold.
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Seven New Character Posters For 'The Hobbit: The Desolation of Smaug'Reported by Outsiders - 11:17 PM 2013.11.03
Still with over a month to go before the official theater release of December 13th and we have seven new character posters for the 'The Hobbit: The Desolation of Smaug' to display... Check them out below.
From Academy Award®-winning filmmaker Peter Jackson comes 'The Hobbit: The Desolation of Smaug.' The second in a trilogy of films that continues the adventure of the title character Bilbo Baggins (Martin Freeman) as he journeys with the Wizard Gandalf (Ian McKellan) and thirteen Dwarves, led by Thorin Oakenshield (Richard Armitage) on an epic quest to reclaim the lost Dwarf Kingdom of Erebor. The three films tell a continuous story set in Middle-earth 60 years before The Lord of the Rings, which Jackson and his filmmaking team brought to the big screen in the blockbuster trilogy that culminated with the Oscar®-winning The Lord of the Rings: The Return of the King. Ian McKellen returns as Gandalf the Grey, with Martin Freeman in the central role of Bilbo Baggins, and Richard Armitage as Thorin Oakenshield. The international ensemble cast is led by Benedict Cumberbatch, Evangeline Lilly, Lee Pace, Luke Evans, Ken Stott, James Nesbitt, and Orlando Bloom as Legolas. The film also stars (in alphabetical order) John Bell, Manu Bennett, Jed Brophy, Adam Brown, John Callen, Ryan Gage, Mark Hadlow, Peter Hambleton, Stephen Hunter, William Kircher, Lawrence Makoare, Sylvester McCoy, Graham McTavish, Dean OGorman, Mikael Persbrandt, and Aidan Turner. The Hobbit: The Desolation of Smaug opens in theaters on December 13th, 2013.
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I"m getting ready to do a lager with dry yeast. Package says to pitch above 68 deg. Then says optimum fermenting temp is 53 deg. Does that mean I pitch at 68 and immediately put in a 53 deg frig. Or do I wait for fermentation to start then go to 53? Should I go to 53 over the course of a few days or in 1 day?
Exactly what yeast is it?
There are a number of ways to skin that cat. I've always pitched at the higher temperature and then, at the very first sign of yeast activity, put the fermenter into the cooler and started dropping the temperature. That's always worked for me. Others chill the wort to fermentation temperature and pitch. Others, still, chill the wort to below fermentation temperature, pitch, and then let the temperature come back up. Everyone reports good results, so choose what sounds most interesting or most comfortable. Personally, I want to try the third method sometime, but I'm always so anxious to pitch my yeast. It's the only part of the process where I have problems being patient. TL
I was just listening to an episode of the Jamil Show about Miabocks yesterday and he uses the 3rd method for what its worth. He said he chills the wort to 40F then pitches and slowly raises the temp to ~50F. I definitely trust his opinion on most things beer.
I'm using Saflager S-23. What would be the benefit of pitching at 40 and warming to 50?