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I really like Reapers and they have huge benefits. They're cheap, they're highly mobile with impassible terrain and they're super fast, you can heal them with meds, and they deal a crap ton of dmg (esp to buildings) their regular attack is like a stim'd marine too just need air cover. If you upgraded their frag grenade thing, you can even take out large groups of enemies ie marauder team in great train robbery. I would hardly call them useless. I used them in outbreak, the last mission in the colonist chain to destroy buildings fast, the first quest where you get them for mining the 8k minerals, on the 2nd to final mission if you choose to destroy their air units against the right and bottom left bases. These are great especially if you race the clock since they demolish things so fast. Ravens definitely had limited use, along with predators. Vultures and hellions were a pain to use because you dont get around to ever repairing them. They are the fastest units in the game though, so i use them for mobility when I get some for free, like deadman's port and the first final mission, entering hell? or something like that. As for cloaking units, I really love banshees. They're so useful, and they demolish ground units. The large amount of detectors was a bit annoying but you just gotta skirt around them and hide in pockets of non detector coverage, or enter from different angles. I usually use vikings as coverfire against air, or have banshees knock out the sunken colonies and then land the vikings to kill the spore colonies etc.. once you kill detectors skirt out the vikings and cloak your banshees and just tear them apart. Firebats were limited use just because they were too slow moving, and their range is bad. I used the flame turrets to replace them as soon as I got the upgrade. wraiths were limited use since they are fairly weak. thor was limited use because they were also too slow moving and bulky. I used BC quite a few times but only 1-2 units max w/ energy shield to tank dmg while my smaller units dealt the dmg (other than on the final mobeous artifact quest where the ripfields would *@%@ my other units) diamondbacks are limited use, (just the train robbery mission) cuz they require a lot of microing and i cant afford the time on brutal, esp if I'm pressed for time goliaths were too weak to utilize, as they die easily, but the spartan company was there if i needed them |
Wraiths. Wraiths. Wraiths. I can't believe how amazingly useless this unit it. Its not even good on its own level, which is hilarious, since each of the levels are literally designed around the introduced unit. Vikings just overshadow these guys so badly.
And speaking of vikings, goliaths are ok, but the cost and the need to repair them made them a lot less appealing, especially in missions to conserve minerals and vespene. Its not that they're a bad unit per say, but they have a lot of other units who do a better job at it. Predator. I got it once. Never again. So unbelievably useless Vultures are nice cannon fodder, especially at 75 minerals a pop. With a tech reactor, you can get a good amount to distract your enemies from your bioball. Barely used Hellions. Only on the Outbreak, but I had Reapers then. |
I love Ravens, I think they're a more useful caster unit than Ghosts. But sadly, they don't measure up to SciVessels with mech-heal.
1) Helion
-> Low HP, can't be healed by medivacs or medics. 2) Predator -> Dies WAY too fast. Should have a ton of HP, be healable by medics to be useful. To balance it out, it's dmg is severly reduced on high armor/hp units. 3) That giant Zerg Transport -> Medivacs I think are better, and does the same function. 4) Medics later on in the campaign -> Medivacs in the air are better healers, not vulnerable to ground aoe fire. 5) Planetary Fortress -> If the AI has gotten to your command center, it's already over. Might be good for expansions, but most missions you won't have time or resources for it. 6) Diamond Backs -> Siege tanks are much better, only one mission where you need to run and gun. |
Ravens definitely have their benefits. I got SV but i find their heals are pretty weak overall and 99% of the time used scvs with double repair
The giant transport is sometimes a pain because you need fusion core to build, but its great because its easier to micro than a fleet of meds and it doesnt kill all your units if it dies, and it has more hp and is faster so it doesnt really die. |
Wraiths, but I still used them to cure my nostalgia feelings. And OMG they do barrel rolls! I kept hoping a hero wraith would show up as a mercenary and have them named like Top Guns or something. Firebats, only used the hero ones.
Edited by Draynam on 8/6/2010 10:20 AM PDT
You guys are completely and absolutely misguided. hummmmmmmmmm BC + SV = gg. You sir are misguided. Sure point defense has uses. But BC + SV is amazing for all attacks. Amazing. |
Truth. Plus build them in mass along side your OP Marines. I can't say that the reapers were useless. They are just very specialized in a way ghosts are. Needed to utilize reapers for an achievement in Outbreak. Which made me sad. I didn't even use them in that mission lol Hellions had spotty their uses for me. Had to mass them in order to get an achievement in Outbreak. Like someone else already said: I really wanted to like these units, but I couldn't find a use to justify their costs. Is this the robo cat? I picked because I thought it looked cool, but never actually used it. Hellions do a better job than them and they don't cost 100 gas a piece. I actually used these guys a bunch. They did really good on the mission where you side with Protoss to kill the infested terran. Too many zerglings and roaches to try to transform vikings on top of. While Vikings are definately better Anti-Air, you have to consider the lose in DPS and bad vulnerability that comes with transforming to hit ground. And then there's cloak. |
You guys are completely and absolutely misguided. I think you are misguided here. Kerrigan one shots battlecruisers. SV can't repair what's dead. But other than that, of course massing the best unit along side an OP unit that can repair the best unit at no resource cost is going to be amazing, but Brutal doesn't really let you do that. And mass Marine-Medic pretty much dominates everything in the campaign, even on brutal.
Edited by Levie on 8/6/2010 10:41 AM PDT
That said, Helions and Wraith are totally useless. Vultures had a use to get an achievement. Planetary Fortress i believed to be very useless, they turned out real great when i found a use for them: Choking with alot of efficiency. I used them in the All In mission on all entrances, and by the time zergs could inflict damage to them, the 5+ Sieges behind each of them deal with any zergs. Nice meatshield. Used them again in the Dig Mission when the stalkers starts justs aiming for my sieges. |
What? Someone thinks Planetary Fortress sucks? You've obviously never used one to try to buff up a choke point, stick 2-3 of those down at a choke point in All In and nothing is getting past it, not even on Hard. I laid 3 down at the west entrance with a couple of bunkers, some tanks, and a few Psi Disrupters and I never had to look at the side again, the Planetary Fortress helped so much.
I gotta say Thor's are also pretty close to useless as well. You need a lot of open ground for them to be of much use, and by the time you get them... well... the missions after that don't offer a whole lot of open ground to use them.
LIMITED USES UNITS ARENT USELESS. Some arbitrary achievement doesn't make vultures any less useless. The delay in the spider mines is infuriating. 90% of them get shot before they even detonate. |
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KJ is in New York prepping for the April 3rd relocation and finance joint committee presentation. (d3j5vwomefv46c.cloudfront.net)
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[–]mymomislizlemon 5 points6 points7 points 1 year ago (0 children)
sac is very lucky to have this man! respect.
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Go get em' KJ
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Gonna be an exciting day tomorrow.
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Hardware news tagged Patriot
Thursday, March 7, 2013
Patriot Reveals Wireless External Hard Disk Drive.
[7:38 pm]
Patriot Launches 500GB, 1TB Wireless HDDs for Mobile Devices
Wednesday, December 19, 2012
Patriot Memory Rolls-Out Ultra-Small "Autobahn" USB Flash Memory Drives.
[10:54 pm]
Patriot Reveals World's Smallest USB Flash Memory Drives
Wednesday, July 18, 2012
Patriot Memory Releases Wi-Fi-Equipped Enclosure for Hard Drives.
[7:14 pm]
Patriot Memory Introduces Gauntlet Node Enclosure for HDDs
Monday, November 28, 2011
AMD to Sell Own-Brand Memory Modules in the U.S.
[9:03 am]
AMD Partners with Patriot Memory, Visiontek for Memory Modules
Thursday, August 4, 2011
Patriot Releases SandForce-Based Pyro-Series Solid-State Drives.
[10:38 pm]
Patriot Launches High-Performance Pyro SSDs with 60GB, 120GB and 240GB Capacities
Tuesday, April 27, 2010
Patriot Launches 2500MHz Memory Modules.
[8:24 pm]
Patriot Unveils World's Fastest Memory Modules
Thursday, February 4, 2010
Patriot Memory Releases 2.25GHz DDR3 Memory Modules.
[8:16 pm]
Patriot Pushes DDR3 Speeds to the Extreme


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The Tax Credit That Saves Lives or Won't?
Expiration of the research tax credit imperils the orphan drug credit, and the lives that depend on it.
July 23, 2014
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The Resilience Hub & Portland Maine Permaculture Message Board › tips and ideas on planting fruit trees
alder |
hello! i'm a virgin tree planter here with some questions on materials...
in an attempt to get a forest garden going, i hastily ordered some fruit trees back in feb from FEDCO (plums, peach, a pear that i'm getting from a friend). well, the pickup weekend is fast approaching and i am still not sure of my placement for these trees. unfortunately, since it was a spur of the moment thing and our snow cover only recently melted, i don't have any areas pre-prepped for them to go into. from what i've gathered, it's not a good idea to add fertilizer or anything too rich to a fruit tree hole upon planting.... fertilizing is typically done a few weeks later once the trees are a bit established. instead it's recommended that the hole be filled in w/ native soil. anyone got thoughts on this, or other methods? agree/disagree? SO, with the above in mind, i'm concerned about what the heck i'm going to fill these holes in. we live at an old granite quarry, my garden is pretty much on an old stone dump where riprap and chunks of stone, large and small, were tossed about. long story short, VERY little soil here.... when i get these holes dug i'm going to be removing lots of rock and hardly any soil. so what do folks recommend i fill in with (besides the little native soil i will have)? i am thinking perhaps a mix of 50:50 peat and compost? would it be wise to toss some lime in with that since the peat is acidic and i've already got pretty acid soil? another option i'm considering it bucketing out some of the rich muck from the quarry..... could that be too rich to plant into? maybe mix that half:half with peat? other ideas? thanks for your thoughts! katie/alder |
zengeos |
Alder, Any chance you can build up things some also? I'll likely follow FEDco's suggestions to some degree, but also incorporate the various ideas outlined here:
agroforestry net Too bad you aren't closer, and I don 't have a good truck. I could really use a few tons of your rocks for my spiral gardens!!! |
Dick H. |
If you can afford it, get a bag of FEDCO tree planting mix for each tree. I've had real good luck with it. Put cardboard down around each tree with seaweed on top of the cardboard. Peace, Dick |
alder |
thanks for your thoughts.... i could start mulching the areas where the trees will go, but i doubt it will do much good (pickup is in 2weeks) and then it'll just be more work to move the mulch, dig the holes, and replace the mulch.
still wondering what to fill the holes with.... i also realized i have 1 year old humanure, and partially broken down leaves. perhaps i could incorporate both of those with some compost and whatever native soil i glean. dick, i can't quite shell out for a bag of tree mix/hole, but i do have most of the ingredients that that mix is composed of. i guess i'll make up something along those lines (not sure of the ratios). and i will definitely be mulching with seaweed!! both under and over the cardboard. seaweed is my mainstay source for creating soil around here. :) any other ideas are welcome! thanks, alder/katie |
David S. |
Mixing in peat and compost should be OK. If there is any subsoil you can mix a little of that in. There is more nutrition in subsoil than most people think. Fine particles from top soil leech down into it. It is often more calcareous than topsoil and so may moderate your ph. Topsoil is usually quite acid and may not hold on to your roots as well as you would want in challenging situations like high winds.
My peach trees are doing excellent in fairly dense soil that is a mix of top soil, sub soil and clay that was created when they excavated for my foundation. I did mix in some peat and composted manure but not a lot and it is likely incorporated by now If it is sunny enough you will probably be fine. |
alder |
hi david, thanks for your reply. this is encouraging.... i will probably end up making a mix of peat, compost, any available native soil, plus some soil scraped from the tops of a few garden beds. you're right that we tend to forget how nutrient-rich subsoil is/can be... i know i am always second-guessing the fertility of what looks to be "poor" soil and am later surprised at how well plants end up doing there.
Aaron P. |
Woody plants need very little in the way of nitrogen, in fact to much is bad for them, so go easy on the manure. What is nice to put into those holes are minerals, like the fedco bags, or azomite or if you don't have the budget for those some gypsum might be good to add some calcium (many fruit trees have a high need of calcium). The reason for not adding much nitrogen is it will stimulate vegetative growth and if that doesn't wind down and stop in time for winter it can cause "winter kill".
zengeos |
So would you NOT recommend amending with the surf n turf compost then?
I had planned on digging the hole as per FEDco recommendations, then mixing about 1/3 compost with soil to boost organic materials, then top dress along the drip line with compost again, with leaves/ leaf mold over top to help retain moisture Then I was planning on planting some Bocking 14 comfrey..2 or 3 around each tree, as well as some onions and garlic and later adding climbing beans to grow up the trees. What else makes good companions for fruit trees? I'm trying to develop a nice little guild for the trees and then transition from that to the veggie garden plots, using the trees as anchors of sorts, with the fruiting shrubs interspersed in the garden to add visual interest and berries, of course. |
Aaron P. |
So would you NOT recommend amending with the surf n turf compost then? That sounds like a great idea, except i wouldn't mix in the compost in your hole, just loosen the soil and add any mineral amendments you might be using and a spadeful of forest soil for beneficial organisms (especially fungi). That compost could be added to your top dressing, where the excess nitrogen will get tied up in the breakdown of leaves and other lingen cellulose material. If you have Sugar maple, flowering dogwood or shagbark hickory in your yard these leaves are probably the best for your top dressing as they (according to Dave Jacke) are accumulators of calcium. |
zengeos |
So I guess I will rake up some leaves from under the sugar maple along the stone wall in the back acre, then, as that area is where I plan to get a lot of my leaves and leaf mold. I can get a few pine needles from way out back for those plants preferring a little more acid, I think, and pine needle mulch as well.
Today, though, I have rhubarb to plant...about a dozen or so roots. Should I dig into the soil at all for that or should I just put a mound of compost down and plant into the compost, then cover with newspaper and leaves? |
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Archive Page - 08/16/13 41 years, 2,178 covers and 55,102 stories from PEOPLE magazine's history for you to enjoy
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- October 23, 1989
- Vol. 32
- No. 17
Publisher's Letter
"We make sure everything fits," says copy processing manager Alan Anuskiewicz, 39, summing up his department's crucial role. Using the layouts created by PEOPLE's page designers and layout artists, the 14-member copy processing staff appropriately "codes" each story, feeding into the magazine's computer system information about the article's proper typeface, point size and column shape and length. Desperate calls from the magazine's editors are a closing-night constant. " 'Tell me there's a coding mistake' is a frequent plea," says Anuskiewicz. "So is, 'Can't you squeeze a few more lines onto the page?' "
The copy processors who field those requests are a diverse group, including Charles Glasser, 31, a Hunter College prelaw student who is also on the varsity fencing team; Donna Cheng, 29, who is studying graphic design at Pratt Institute; and accomplished tuba player Jennifer Paradis-Hagar, 34. Working up to 12-hour shifts, often through the night, department members survive the pressures by downing an estimated 15 pots of coffee and taking early-morning strolls through otherwise abandoned hallways. "The toughest nights are those when there's a sudden cover change," says Anuskiewicz, citing last month's quick-switch to convicted tax evader Leona Helmsley as a story that demanded a Herculean effort from all staff members.
Though layouts start trickling in on Thursday, the big crunch begins Tuesday morning at 10 and continues until about 6 A.M. Thursday when that week's issue is finally put to bed. "We call it the Tuesday night bloodbath," says Anthony Zarvos, 38, deputy copy processing manager. "But it's kind of challenging knowing that you have so little time to do so much."
After PEOPLE's copy editors correct for style and grammar, the stories are returned to copy processing for final adjustment. "The copy editors make sure it reads correctly," says Key Martin, 46, a 10-year department veteran, "but we make sure it looks right."
After giving the pages a final scan, the staff transmits them electronically to be merged with the photographs at the magazine's engravers and sent via satellite to PEOPLE's printing presses around the country.
"When we hit that button," says Zarvos, "it's got to be right."
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Guest blog post by Tracy Ahrens, author of "Raising My Furry Children"
It's a Pittie Rescue (Peotone, Ill.) faced great sadness the morning of April 7 as their rescued pit bull, Mama Petunia, died suddenly while in veterinary care.
"The rescue is in shock, I am in shock," said It's a Pittie volunteer, Kathy DeMase of Crete. "She deserved so much more. She deserved a chance to know a life without pain, with a warm bed, a fully tummy and more love than 10 hearts can hold."
The pregnant, 3-year-old pit bull was found approximately March 23. A couple in Park Forest spotted her as she was walking in a neighborhood, according to Debbie Wilke, of Peotone, president and co-founder of It's a Pittie Rescue. They could tell she was injured and kept her in a yard until police came. Petunia was taken to a shelter that holds strays and was there for five days before It's a Pittie Rescue was notified.
It was clear to Dr. Lynlee Wessels of Animal Wellness Center of Monee, that Petunia sustained blunt force trauma. Her teeth were fractured and toes on her right front foot were all broken. Petunia was also emaciated and had sustained burns over 25 percent of her body. Those burns, according to Wessels, were between one and two weeks old.
Within days, Petunia gave birth to 10 puppies. Five were stillborn. Wessels believes it was probably Petunia's first litter of puppies. Despite her traumatic past and painful wounds, Petunia nursed her five puppies and rested calmly in the care of countless rescue workers and veterinary technicians.
Petunia had been transported from veterinary care at Animal Wellness Center of Monee to a specialty facility. She hadn't eaten for at least three days but was able to drink some water. Veterinarians noticed she was having trouble, DeMase said, so they ran several tests. Her blood sugar was low and then her heart just stopped. "They performed CPR for 10 minutes, tried massaging her heart, gave her medications, but to no avail," DeMase said.
Petunia had been receiving hydrotherapy and pain medications for her burns and was handling nursing her puppies very well. It was estimated that she would need up to 12 weeks of care for her wounds to heal.
On April 5 her Facebook page noted that Petunia wasn't feeling well. She had a slight fever and her appetite was minimal. A veterinarian thought she could have a blockage in her stomach. Exploratory surgery revealed no major blockage, but Petunia had fluid in her body that needed to be drained. Concerns over a uterine infection led a veterinarian to spay Petunia while she was under anesthesia.Petunia and her story has attracted over 11,000 likes on her Facebook page.
Her puppies (three boys and two girls) recently were named Huckleberry, Snapdragon (Snappy), Phoenix Caine, Jasmine and Tulip. They are now being bottle-fed.
Her Facebook page post April 7 noted for the world to see: "My little body suffered major trauma and I stayed strong as long as I could. I felt the love that surrounded me and I got my babies to a safe place, I knew it was okay to let go. I am now free of pain and suffering and will be reunited with my 5 little ones that didn't make. Thank you for showing me love and compassion. May God rest my soul in peace."
Donations to help pay for Petunia's past medical care and for the ongoing care of her puppies are being accepted HERE.
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Filed under: animal cruelty, dogs, pets
Tags: animal abuse, animal cruelty, dog is burned, It's a Pittie Rescue, Petunia, Pit Bull, pit bull puppies, Raising My Furry Children, Steve Dale archives, Tracy Ahrens


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Latest Bicycles Stories
Recumbent bikes are the staple gym piece to allow everyone to enjoy fitness. West Babylon, NY (PRWEB) November 03, 2014 Pro Gym Supply believes recumbent
The pry-bar and bolt-cutter resistant bike carrier allows for flexibility and security with a locking steel security bar and fully adjustable frame trays SPOKANE, Wash., Sept.
SEATTLE, July 15, 2014 /PRNewswire/ -- Northwest locals know that summer officially arrives July 5, following an often overcast Fourth of July, but weather rarely keeps us from enjoying the
Landice, the leading made-in-the-USA treadmill manufacturer, announced the introduction of their new line of upright and recumbent bikes for home and light commercial applications.
Nicolai USA has announced that the individually crafted mountain bikes made famous by Kalle Nicolai are now available in the U.S. Los Angeles, CA (PRWEB) June
Marine Corps veteran LeMar Best sustained traumatic injuries during his service, but his participation in the annual
MIT-spinoff Superpedestrian unveiled a commercial version of its Copenhagen Wheel on Tuesday, allowing consumers to turn any ordinary bicycle into a hybrid "e-bike."
This Cycling Control System device enables stroke survivors, amputees and other disabled users to shift gears, steer, and brake on a recumbent tricycle using only one hand.
Stealth Electric Bikes has announced the enhancement of high performance upgrades and standard features for the Spring 2013 editions of The Bomber, The Fighter and The Hurricane Stealth Electric
Their newest Tricycle, the Meteor™ 3-Wheel Recumbent Tricycle, is a Delta-style recumbent trike that includes the classic styling that defines the Trailmate products.
- Small missiles, especially grape, canister, fragments of iron, and the like, when fired, as upon an enemy at close quarters.
- To fire mitraille at.


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Taste of Hermantown serves up a feast
Published 10/20/2012, Duluth Budgeteer News
With a room full of ham, chicken wings and about two dozen other food samples, the annual Taste of Hermantown will open for patrons this Thursday from 5-7 p.m. at the Shriner's Building in Hermantown at 5152 Miller Trunk Hwy.
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Thats sort of what I was leaning towards saying. It would be a good to have everybody unformally running the same script, I would choose Glype, with it all ompitizmed. Remove Disk I/O by turning Apache logs off, caching off being mandatory, and have all them uniformally writing to the same Custom Glype log file making abuse issues easier, since your have just one log file with unencrypted urls all listed by date.
There is also many function and systems calls in the glype script that can be removed too. For example in the index.php this,
That is a useless system call on each view of index.php and could just be deleted.PHP Code:
* Check PHP version
if ( version_compare(PHP_VERSION, 5) < 0 ) {
$themeReplace['error'] = '
You need PHP 5 to run this script. You are currently running ' . PHP_VERSION . '
';


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Ryan Fitzpatrick QB HOU Aug 20 '7810 Comments
Bills GM Buddy Nix reiterated that he plans to draft a franchise quarterback in 2013.
"Let me be as honest as I can," Nix said. "I think we really need to address it this year. The thing we can't do is you can't create one. You can't go out, thinking 'I've got to make this guy a player.' If you do, then you're going to be in a bigger mess." It's the second time in a week that Nix has made it clear Ryan Fitzpatrick is no longer viewed as the quarterback of the future. Fitzpatrick has a $3 million roster bonus on top of a $4.25 million base salary next season.


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Mar 19, 2012, 04:42 AM | |||
A vid of some micro jet mayhem from the weekend. Nitro brought along his Micro Sabre and we had 2 SkyAngel Mig 15 to compare. Migs are alot faster but a hell of a lot harder to fly. The Sabre is rock steady and looks much nicer to boot. Score 1 win for the Sabre. Well done Great Planes
Mar 19, 2012, 05:55 AM | |
Joined Mar 2010
156 Posts
Well I go my Mig 29 today, and I've mixed feelings.
First off, I wish they hadn't painted it for me. They put so much red paint on that it has caused terrible warping of the wings and vertical stabs. These have paint mainly on the one side and, as they should know, paint shrinks when it dries, causing it to bend the foam. Another complaint is that the fans have been taken out and re-glued in. I can tell since there is glue and foam impressions on the top (when looking upside down) with the motor covers off so I'm not sure if I should be concerned about this. The last real complaint is that there is no on-board mixing of the servos. While one servo wire is labeled elevator and another ailerons, one essentially controls one servo, and the other controls the other servo. Is this the same for others who have this? While I can deal with this by mixing on the transmitter, it's somewhat disappointing. Apart from that, it looks like a nice little plane, just disappointing that I just can never seem to get exactly what I think I ordered from HK. |
Mar 19, 2012, 12:03 PM | |
Canada, QC, Pointe-Claire
Joined Jun 2011
525 Posts
Hello all
After a winter of hibernation, i brought out my Micro Jet F-86 for some fun. First i replaced the original receiver with and Orange DSM2 so i could use it with my DX8. With the 7.4v 350mah battery the plane is perfectly balanced. After many flights, and of course the last one which ended up with "uncontrolled flight into terrain" i am in need of parts!! Specifically the canopy which could not be found? Other that HK (who do not have stock on the canopy) are there other sources out there? Such a fun little plane to fly, seems like a real shame to put it on the side lines because of that. Any help is appreciated! |
Mar 19, 2012, 05:49 PM | |
I have 5 of the SA 35mm and many of the 50mm they are all great fun, yes some need a little attention upon arrival which I have come to excpect BUT once all the smmal things are in order these little jets are so much fun. I think that if any on-board mixing would just add un-needed weight. All jets I have use the Tx for mixing? I do undrestand your frustration but the extra work is just part of the hobby, right?
Mar 19, 2012, 06:59 PM | ||
Italy, Sicily, Palermo
Joined Sep 2006
881 Posts
esc ? Lipo ? |
Mar 19, 2012, 07:54 PM | ||
Joined Mar 2010
156 Posts
As for the mixing, it's really not an issue, but why not label the wires correctly. It wouldn't take much effort at all, and would go a long way towards a good first impression. Has anyone tried putting the wheels on? If so, how is it taking off from gravel with them? I'm not sure which way to go, but probably no wheels and just a grass lander. Knowing my luck it'll probably be a lawn dart first flight anyway (no need for wheels then) ;-) |
Mar 19, 2012, 10:26 PM | ||
I would keep them as light as possible, so 86 the landing gear. Yeah I have gotten more than one jet with crazy warping it seems that they should be able to ge that part right huh?. Well to be honsest the best 35mm flyer I have would have to be the T45 on 3S not 2S. It kinda just flops around on 2S but the 3S really makes it a great bird. The Mig and the Saber are great too but also on 3S. The should have come 3S ready outta the box, but again you gotta put some work into'em to get a great bird. Good luck on maiden just watch the thrust angle |
Mar 23, 2012, 10:55 AM | ||
I have the MiG-29 and a 64mm Exceed (nitroplanes) F-35. the larger F-35 because it's all gray is harder to see. Also the MiG-29 can fly more comfortably in smaller areas. |
Mar 23, 2012, 01:10 PM | ||
United States, TN, Jackson
Joined Mar 2007
4,084 Posts
Let me know!!! Kevin |
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To the Editor: County to raise property taxes
Why on earth would the Winnebago County Board vote to raise county property taxes? Last year, the county had a record number of tax protests, and even Chairman Christiansen encouraged people to protest if they thought their taxes were too high. Now, one year later—with local tax rates in the area of 8 percent or more (national average is around 2 percent, according to an AARP news article), and local unemployment at an all-time high, they decide they need to raise property taxes again. If they're wondering why it seemed no one objected, then just remember the "thousands" of objections last year in the form of "tax protest forms." I think most of our county board members are out of touch with the reality of life in Rockford and the surrounding county. I hope they will come to their senses and repeal the tax hike. Well, that's my two cents!
Mike Castronovo
From the Sept. 29-Oct. 5, 2010 issue


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Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain 129 H2 Haplotype g7 Generation N15+N1F1pN1
Generation Definitions Donating Investigator Dr. Jeffrey A. Bluestone, University of California, San Francisco Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcDescription
A "knock-in" replaces the endogenous gene with an Il4/IRES/EGFP bicistronic construct, which places both IL4 and EGFP expression under the control of the endogenous Il4 gene regulatory elements. IL4 activity in these mutant mice remains intact. Cells activated to express IL4 also express EGFP allowing reliable in vivo tracking of both innate and adaptive immune cells and enabling their isolation without further stimulation. Because the IRES element promotes translational competence capable of revealing low-level transcription otherwise not apparent from the canonical 5'-cap of the mRNA, cells from these mice also can be used to report competence for IL4 production upon stimulation. This knock-in allele has been backcrossed to NOD for 15 generations. Diabetes incidence in mutant mice is reported to be about 60%, which is not statistically different from wild-type cohorts or NOD inbred mice.Development
IL4, interleukin4, located on Chr.11, affects the growth and differentiation of B, T, and mast cells, and, IL4 receptors are expressed on B cells, T cells, mast cells, and macrophages. A targeting vector containing herpes simplex virus thymidine kinase, Il4 exon 3-4 sequence, a loxP-flanked neomycin cassette, and an IRES-EGFP construct was utilized in the making of this mutant. The vector was electroporated into 129T2 X 129S4-derived PrmCre embryonic stem (ES) cells. These ES cells express Cre recombinase under the direction of the mouse protamine 1 promoter (expresses in testis/male germline). Correctly targeted ES cells were injected into C57BL/6 blastocysts. The Il4tm1Lky mutation was transferred from a C57BL/6 background to NOD through 15 backcross generations. In 2007, the T1DR received hemizygous mice at generation N15 and mated to NOD/ShiLtJ prior to mating wild-type x hemizygous.
Control | ||
Wild-type from the colony | ||
001976 NOD/ShiLtJ | ||
Considerations for Choosing Controls |
Fluorescent Protein StrainsView Fluorescent Protein Strains (387 strains)Strains carrying Il4tm1Lky allele
004190 C.129-Il4tm1Lky/J View Strains carrying Il4tm1Lky (1 strain)Strains carrying other alleles of GFPView Strains carrying other alleles of GFP (371 strains)Strains carrying other alleles of Il4
002253 B6.129P2-Il4tm1Cgn/J 002496 BALB/c-Il4tm2Nnt/J 015859 C.129P2(Cg)-Il4/Il13tm1.1Lky/J 003480 C.129S2(B6)-Il4tm1Gru/J 002518 C57BL/6-Il4tm1Nnt/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J 005879 D1Lac.Cg-Il4tm1Cgn/J 002574 NOD.129P2(B6)-Il4tm1Cgn/Dvs 004222 NOD.129P2(B6)-Il4tm1Cgn/DvsJ 004291 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ View Strains carrying other alleles of Il4 (10 strains)
Fluorescent Proteins/lacZ Systems
View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
GFP relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
Immunology, Inflammation and Autoimmunity Research
Type 1 Diabetes
Intracellular Signaling Molecules
Research Tools
Fluorescent Proteins
Immunology, Inflammation and Autoimmunity Research
Il4tm1Lky relatedResearch Tools
Fluorescent Proteins
Cancer Research
Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Allele Symbol | Il4tm1Lky | ||
Allele Name | targeted mutation 1, Richard M Locksley | ||
Allele Type | Targeted (Reporter) | ||
Common Name(s) | 4 get; 4-get; 4get; | ||
Mutation Made By | Markus Mohrs, Trudeau Institute, Inc. | ||
Site of Expression | Cells activated to express IL-4 will express GFP. IL-4 activity remains intact. | ||
Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
Gene Symbol and Name | Il4, interleukin 4 | ||
Chromosome | 11 | ||
Gene Common Name(s) | BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Il-4; Il4e12; | ||
Molecular Note | A loxP-flanked neomycin cassette followed by an internal ribosomal entry site (IRES) and an EGFP gene was inserted into sequences corresponding to the 3' untranslated region in exon 4. The neomycin cassette was removed by Cre mediated germline recombination in chimeric mice to generate the final allele. These mice express green fluorescent protein under the control of the Il4 promoter. [MGI Ref ID J:75415] | ||
Genotyping Protocols
Il4tm1Lky, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
Il4tm1Lky relatedAu-Yeung BB; Fowell DJ. 2007. A key role for Itk in both IFN gamma and IL-4 production by NKT cells. J Immunol 179(1):111-9. [PubMed: 17579028] [MGI Ref ID J:143156]
Au-Yeung BB; Katzman SD; Fowell DJ. 2006. Cutting edge: Itk-dependent signals required for CD4+ T cells to exert, but not gain, Th2 effector function. J Immunol 176(7):3895-9. [PubMed: 16547221] [MGI Ref ID J:129883]
Cain JA; Smith JA; Ondr JK; Wang B; Katz JD. 2006. NKT cells and IFN-gamma establish the regulatory environment for the control of diabetogenic T cells in the nonobese diabetic mouse. J Immunol 176(3):1645-54. [PubMed: 16424194] [MGI Ref ID J:126603]
Cheng LE; Hartmann K; Roers A; Krummel MF; Locksley RM. 2013. Perivascular mast cells dynamically probe cutaneous blood vessels to capture immunoglobulin e. Immunity 38(1):166-75. [PubMed: 23290520] [MGI Ref ID J:193023]
Cheng LE; Wang ZE; Locksley RM. 2010. Murine B cells regulate serum IgE levels in a CD23-dependent manner. J Immunol 185(9):5040-7. [PubMed: 20870945] [MGI Ref ID J:165198]
Clay BS; Shilling RA; Bandukwala HS; Moore TV; Cannon JL; Welcher AA; Weinstock JV; Sperling AI. 2009. Inducible costimulator expression regulates the magnitude of Th2-mediated airway inflammation by regulating the number of Th2 cells. PLoS One 4(11):e7525. [PubMed: 19888475] [MGI Ref ID J:155428]
Dolgachev V; Petersen BC; Budelsky AL; Berlin AA; Lukacs NW. 2009. Pulmonary IL-17E (IL-25) production and IL-17RB+ myeloid cell-derived Th2 cytokine production are dependent upon stem cell factor-induced responses during chronic allergic pulmonary disease. J Immunol 183(9):5705-15. [PubMed: 19828636] [MGI Ref ID J:156804]
Everts B; Hussaarts L; Driessen NN; Meevissen MH; Schramm G; van der Ham AJ; van der Hoeven B; Scholzen T; Burgdorf S; Mohrs M; Pearce EJ; Hokke CH; Haas H; Smits HH; Yazdanbakhsh M. 2012. Schistosome-derived omega-1 drives Th2 polarization by suppressing protein synthesis following internalization by the mannose receptor. J Exp Med 209(10):1753-67, S1. [PubMed: 22966004] [MGI Ref ID J:191421]
Everts B; Perona-Wright G; Smits HH; Hokke CH; van der Ham AJ; Fitzsimmons CM; Doenhoff MJ; van der Bosch J; Mohrs K; Haas H; Mohrs M; Yazdanbakhsh M; Schramm G. 2009. Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses. J Exp Med 206(8):1673-80. [PubMed: 19635864] [MGI Ref ID J:151482]
Fairfax KC; Amiel E; King IL; Freitas TC; Mohrs M; Pearce EJ. 2012. IL-10R blockade during chronic schistosomiasis mansoni results in the loss of B cells from the liver and the development of severe pulmonary disease. PLoS Pathog 8(1):e1002490. [PubMed: 22291593] [MGI Ref ID J:195404]
Fallon PG; Ballantyne SJ; Mangan NE; Barlow JL; Dasvarma A; Hewett DR; McIlgorm A; Jolin HE; McKenzie AN. 2006. Identification of an interleukin (IL)-25-dependent cell population that provides IL-4, IL-5, and IL-13 at the onset of helminth expulsion. J Exp Med 203(4):1105-16. [PubMed: 16606668] [MGI Ref ID J:123748]
Fukuyama T; Kasper LH; Boussouar F; Jeevan T; van Deursen J; Brindle PK. 2009. Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29(14):3894-904. [PubMed: 19433445] [MGI Ref ID J:150146]
Gagliani N; Magnani CF; Huber S; Gianolini ME; Pala M; Licona-Limon P; Guo B; Herbert DR; Bulfone A; Trentini F; Di Serio C; Bacchetta R; Andreani M; Brockmann L; Gregori S; Flavell RA; Roncarolo MG. 2013. Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat Med 19(6):739-46. [PubMed: 23624599] [MGI Ref ID J:198570]
Gao Y; Nish SA; Jiang R; Hou L; Licona-Limon P; Weinstein JS; Zhao H; Medzhitov R. 2013. Control of T helper 2 responses by transcription factor IRF4-dependent dendritic cells. Immunity 39(4):722-32. [PubMed: 24076050] [MGI Ref ID J:208202]
Girtsman T; Jaffar Z; Ferrini M; Shaw P; Roberts K. 2010. Natural Foxp3(+) regulatory T cells inhibit Th2 polarization but are biased toward suppression of Th17-driven lung inflammation. J Leukoc Biol 88(3):537-46. [PubMed: 20495073] [MGI Ref ID J:164926]
Goh YP; Henderson NC; Heredia JE; Red Eagle A; Odegaard JI; Lehwald N; Nguyen KD; Sheppard D; Mukundan L; Locksley RM; Chawla A. 2013. Eosinophils secrete IL-4 to facilitate liver regeneration. Proc Natl Acad Sci U S A 110(24):9914-9. [PubMed: 23716700] [MGI Ref ID J:197388]
Hadeiba H; Locksley RM. 2003. Lung CD25 CD4 regulatory T cells suppress type 2 immune responses but not bronchial hyperreactivity. J Immunol 170(11):5502-10. [PubMed: 12759427] [MGI Ref ID J:109990]
Hammad H; Plantinga M; Deswarte K; Pouliot P; Willart MA; Kool M; Muskens F; Lambrecht BN. 2010. Inflammatory dendritic cells--not basophils--are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen. J Exp Med 207(10):2097-111. [PubMed: 20819925] [MGI Ref ID J:165817]
Hams E; McCarron MJ; Amu S; Yagita H; Azuma M; Chen L; Fallon PG. 2011. Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells. J Immunol 186(10):5648-55. [PubMed: 21490158] [MGI Ref ID J:173223]
Harris DP; Goodrich S; Mohrs K; Mohrs M; Lund FE. 2005. Cutting edge: the development of IL-4-producing B cells (B effector 2 cells) is controlled by IL-4, IL-4 receptor alpha, and Th2 cells. J Immunol 175(11):7103-7. [PubMed: 16301612] [MGI Ref ID J:122200]
Haynes NM; Allen CD; Lesley R; Ansel KM; Killeen N; Cyster JG. 2007. Role of CXCR5 and CCR7 in follicular Th cell positioning and appearance of a programmed cell death gene-1high germinal center-associated subpopulation. J Immunol 179(8):5099-108. [PubMed: 17911595] [MGI Ref ID J:153037]
Heib V; Becker M; Warger T; Rechtsteiner G; Tertilt C; Klein M; Bopp T; Taube C; Schild H; Schmitt E; Stassen M. 2007. Mast cells are crucial for early inflammation, migration of Langerhans cells, and CTL responses following topical application of TLR7 ligand in mice. Blood 110(3):946-53. [PubMed: 17446350] [MGI Ref ID J:145396]
Heredia JE; Mukundan L; Chen FM; Mueller AA; Deo RC; Locksley RM; Rando TA; Chawla A. 2013. Type 2 innate signals stimulate fibro/adipogenic progenitors to facilitate muscle regeneration. Cell 153(2):376-88. [PubMed: 23582327] [MGI Ref ID J:214506]
Huber S; Hoffmann R; Muskens F; Voehringer D. 2010. Alternatively activated macrophages inhibit T-cell proliferation by Stat6-dependent expression of PD-L2. Blood 116(17):3311-20. [PubMed: 20625006] [MGI Ref ID J:165869]
Jang S; Schaller M; Berlin AA; Lukacs NW. 2010. Notch Ligand Delta-Like 4 Regulates Development and Pathogenesis of Allergic Airway Responses by Modulating IL-2 Production and Th2 Immunity. J Immunol 185(10):5835-44. [PubMed: 20944009] [MGI Ref ID J:165624]
Katzman SD; Fowell DJ. 2008. Pathogen-imposed skewing of mouse chemokine and cytokine expression at the infected tissue site. J Clin Invest 118(2):801-11. [PubMed: 18188454] [MGI Ref ID J:131191]
Kim-Saijo M; Janssen EM; Sugie K. 2008. CD4 cell-secreted, posttranslationally modified cytokine GIF suppresses Th2 responses by inhibiting the initiation of IL-4 production. Proc Natl Acad Sci U S A 105(49):19402-7. [PubMed: 19036925] [MGI Ref ID J:142099]
King IL; Amiel E; Tighe M; Mohrs K; Veerapen N; Besra G; Mohrs M; Leadbetter EA. 2013. The mechanism of splenic invariant NKT cell activation dictates localization in vivo. J Immunol 191(2):572-82. [PubMed: 23785119] [MGI Ref ID J:205445]
King IL; Mohrs M. 2009. IL-4-producing CD4+ T cells in reactive lymph nodes during helminth infection are T follicular helper cells. J Exp Med 206(5):1001-7. [PubMed: 19380638] [MGI Ref ID J:148500]
King SB; Knorn AM; Ohnmacht C; Voehringer D. 2008. Accumulation of effector CD4 T cells during type 2 immune responses is negatively regulated by Stat6. J Immunol 180(2):754-63. [PubMed: 18178813] [MGI Ref ID J:130967]
Lazarski CA; Ford J; Katzman SD; Rosenberg AF; Fowell DJ. 2013. IL-4 attenuates Th1-associated chemokine expression and Th1 trafficking to inflamed tissues and limits pathogen clearance. PLoS One 8(8):e71949. [PubMed: 23991011] [MGI Ref ID J:206420]
Lee J; Kim TH; Murray F; Li X; Choi SS; Broide DH; Corr M; Lee J; Webster NJ; Insel PA; Raz E. 2015. Cyclic AMP concentrations in dendritic cells induce and regulate Th2 immunity and allergic asthma. Proc Natl Acad Sci U S A 112(5):1529-34. [PubMed: 25605931] [MGI Ref ID J:217659]
Liang HE; Reinhardt RL; Bando JK; Sullivan BM; Ho IC; Locksley RM. 2011. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol 13(1):58-66. [PubMed: 22138715] [MGI Ref ID J:178986]
Licona-Limon P; Henao-Mejia J; Temann AU; Gagliani N; Licona-Limon I; Ishigame H; Hao L; Herbert DR; Flavell RA. 2013. Th9 Cells Drive Host Immunity against Gastrointestinal Worm Infection. Immunity 39(4):744-57. [PubMed: 24138883] [MGI Ref ID J:208730]
Liu AY; Dwyer DF; Jones TG; Bankova LG; Shen S; Katz HR; Austen KF; Gurish MF. 2013. Mast Cells Recruited to Mesenteric Lymph Nodes during Helminth Infection Remain Hypogranular and Produce IL-4 and IL-6. J Immunol 190(4):1758-66. [PubMed: 23319739] [MGI Ref ID J:193314]
Liu X; Yan X; Zhong B; Nurieva RI; Wang A; Wang X; Martin-Orozco N; Wang Y; Chang SH; Esplugues E; Flavell RA; Tian Q; Dong C. 2012. Bcl6 expression specifies the T follicular helper cell program in vivo. J Exp Med 209(10):1841-52, S1-24. [PubMed: 22987803] [MGI Ref ID J:191271]
Loke P; Gallagher I; Nair MG; Zang X; Brombacher F; Mohrs M; Allison JP; Allen JE. 2007. Alternative activation is an innate response to injury that requires CD4+ T cells to be sustained during chronic infection. J Immunol 179(6):3926-36. [PubMed: 17785830] [MGI Ref ID J:152038]
Loke P; Zang X; Hsuan L; Waitz R; Locksley RM; Allen JE; Allison JP. 2005. Inducible costimulator is required for type 2 antibody isotype switching but not T helper cell type 2 responses in chronic nematode infection. Proc Natl Acad Sci U S A 102(28):9872-7. [PubMed: 15994233] [MGI Ref ID J:99858]
Macleod MK; McKee AS; David A; Wang J; Mason R; Kappler JW; Marrack P. 2011. Vaccine adjuvants aluminum and monophosphoryl lipid A provide distinct signals to generate protective cytotoxic memory CD8 T cells. Proc Natl Acad Sci U S A 108(19):7914-9. [PubMed: 21518876] [MGI Ref ID J:172191]
McHedlidze T; Waldner M; Zopf S; Walker J; Rankin AL; Schuchmann M; Voehringer D; McKenzie AN; Neurath MF; Pflanz S; Wirtz S. 2013. Interleukin-33-dependent innate lymphoid cells mediate hepatic fibrosis. Immunity 39(2):357-71. [PubMed: 23954132] [MGI Ref ID J:208227]
McKee AS; MacLeod M; White J; Crawford F; Kappler JW; Marrack P. 2008. Gr1+IL-4-producing innate cells are induced in response to Th2 stimuli and suppress Th1-dependent antibody responses. Int Immunol 20(5):659-69. [PubMed: 18343889] [MGI Ref ID J:134492]
McKee AS; Munks MW; MacLeod MK; Fleenor CJ; Van Rooijen N; Kappler JW; Marrack P. 2009. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 183(7):4403-14. [PubMed: 19734227] [MGI Ref ID J:152787]
Mingueneau M; Roncagalli R; Gregoire C; Kissenpfennig A; Miazek A; Archambaud C; Wang Y; Perrin P; Bertosio E; Sansoni A; Richelme S; Locksley RM; Aguado E; Malissen M; Malissen B. 2009. Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor. Immunity 31(2):197-208. [PubMed: 19682930] [MGI Ref ID J:151840]
Mohrs K; Harris DP; Lund FE; Mohrs M. 2005. Systemic dissemination and persistence of Th2 and type 2 cells in response to infection with a strictly enteric nematode parasite. J Immunol 175(8):5306-13. [PubMed: 16210636] [MGI Ref ID J:119109]
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Nel HJ; Hams E; Saunders SP; Mangan NE; Smith P; Atzberger A; Flavell RA; Akira S; McKenzie AN; Fallon PG. 2011. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice. J Immunol 186(8):4631-9. [PubMed: 21398616] [MGI Ref ID J:172526]
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Ohnmacht C; Schwartz C; Panzer M; Schiedewitz I; Naumann R; Voehringer D. 2010. Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths. Immunity 33(3):364-74. [PubMed: 20817571] [MGI Ref ID J:164432]
Ohnmacht C; Voehringer D. 2009. Basophil effector function and homeostasis during helminth infection. Blood 113(12):2816-25. [PubMed: 18941115] [MGI Ref ID J:146344]
Palm NW; Rosenstein RK; Yu S; Schenten DD; Florsheim E; Medzhitov R. 2013. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity. Immunity 39(5):976-85. [PubMed: 24210353] [MGI Ref ID J:208996]
Parravicini V; Field AC; Tomlinson PD; Albert Basson M; Zamoyska R. 2008. Itch-/- alphabeta and gammadelta T cells independently contribute to autoimmunity in Itchy mice. Blood 111(8):4273-7282. [PubMed: 18256323] [MGI Ref ID J:134355]
Patnode ML; Cheng CW; Chou CC; Singer MS; Elin MS; Uchimura K; Crocker PR; Khoo KH; Rosen SD. 2013. Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue. J Biol Chem 288(37):26533-45. [PubMed: 23880769] [MGI Ref ID J:203546]
Perona-Wright G; Mohrs K; Mayer KD; Mohrs M. 2010. Differential regulation of IL-4Ralpha expression by antigen versus cytokine stimulation characterizes Th2 progression in vivo. J Immunol 184(2):615-23. [PubMed: 20018622] [MGI Ref ID J:159399]
Perona-Wright G; Mohrs K; Mohrs M. 2010. Sustained signaling by canonical helper T cell cytokines throughout the reactive lymph node. Nat Immunol 11(6):520-6. [PubMed: 20418876] [MGI Ref ID J:160618]
Perona-Wright G; Mohrs K; Taylor J; Zaph C; Artis D; Pearce EJ; Mohrs M. 2008. Cutting edge: Helminth infection induces IgE in the absence of mu- or delta-chain expression. J Immunol 181(10):6697-701. [PubMed: 18981085] [MGI Ref ID J:140953]
Petersen BC; Budelsky AL; Baptist AP; Schaller MA; Lukacs NW. 2012. Interleukin-25 induces type 2 cytokine production in a steroid-resistant interleukin-17RB+ myeloid population that exacerbates asthmatic pathology. Nat Med 18(5):751-8. [PubMed: 22543263] [MGI Ref ID J:185138]
Phythian-Adams AT; Cook PC; Lundie RJ; Jones LH; Smith KA; Barr TA; Hochweller K; Anderton SM; Hammerling GJ; Maizels RM; MacDonald AS. 2010. CD11c depletion severely disrupts Th2 induction and development in vivo. J Exp Med 207(10):2089-96. [PubMed: 20819926] [MGI Ref ID J:194809]
Piehler D; Stenzel W; Grahnert A; Held J; Richter L; Kohler G; Richter T; Eschke M; Alber G; Muller U. 2011. Eosinophils Contribute to IL-4 Production and Shape the T-Helper Cytokine Profile and Inflammatory Response in Pulmonary Cryptococcosis. Am J Pathol 179(2):733-44. [PubMed: 21699881] [MGI Ref ID J:174409]
Price AE; Liang HE; Sullivan BM; Reinhardt RL; Eisley CJ; Erle DJ; Locksley RM. 2010. Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc Natl Acad Sci U S A 107(25):11489-94. [PubMed: 20534524] [MGI Ref ID J:161387]
Rangel-Moreno J; Moyron-Quiroz JE; Carragher DM; Kusser K; Hartson L; Moquin A; Randall TD. 2009. Omental milky spots develop in the absence of lymphoid tissue-inducer cells and support B and T cell responses to peritoneal antigens. Immunity 30(5):731-43. [PubMed: 19427241] [MGI Ref ID J:149548]
Reese TA; Liang HE; Tager AM; Luster AD; Van Rooijen N; Voehringer D; Locksley RM. 2007. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 447(7140):92-6. [PubMed: 17450126] [MGI Ref ID J:122735]
Reinhardt RL; Hong S; Kang SJ; Wang ZE; Locksley RM. 2006. Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol 177(3):1618-27. [PubMed: 16849470] [MGI Ref ID J:112745]
Reinhardt RL; Liang HE; Locksley RM. 2009. Cytokine-secreting follicular T cells shape the antibody repertoire. Nat Immunol 10(4):385-93. [PubMed: 19252490] [MGI Ref ID J:147784]
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Sofi MH; Qiao Y; Ansel KM; Kubo M; Chang CH. 2011. Induction and Maintenance of IL-4 Expression Are Regulated Differently by the 3' Enhancer in CD4 T Cells. J Immunol 186(5):2792-9. [PubMed: 21282512] [MGI Ref ID J:169377]
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Sokol CL; Chu NQ; Yu S; Nish SA; Laufer TM; Medzhitov R. 2009. Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 response. Nat Immunol 10(7):713-20. [PubMed: 19465907] [MGI Ref ID J:150141]
Sullivan BM; Liang HE; Bando JK; Wu D; Cheng LE; McKerrow JK; Allen CD; Locksley RM. 2011. Genetic analysis of basophil function in vivo. Nat Immunol 12(6):527-35. [PubMed: 21552267] [MGI Ref ID J:172122]
Tang H; Cao W; Kasturi SP; Ravindran R; Nakaya HI; Kundu K; Murthy N; Kepler TB; Malissen B; Pulendran B. 2010. The T helper type 2 response to cysteine proteases requires dendritic cell-basophil cooperation via ROS-mediated signaling. Nat Immunol 11(7):608-17. [PubMed: 20495560] [MGI Ref ID J:161857]
Tyznik AJ; Verma S; Wang Q; Kronenberg M; Benedict CA. 2014. Distinct requirements for activation of NKT and NK cells during viral infection. J Immunol 192(8):3676-85. [PubMed: 24634489] [MGI Ref ID J:210013]
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Voehringer D; Reese TA; Huang X; Shinkai K; Locksley RM. 2006. Type 2 immunity is controlled by IL-4/IL-13 expression in hematopoietic non-eosinophil cells of the innate immune system. J Exp Med 203(6):1435-46. [PubMed: 16702603] [MGI Ref ID J:124383]
Voehringer D; Rosen DB; Lanier LL; Locksley RM. 2004. CD200 receptor family members represent novel DAP12-associated activating receptors on basophils and mast cells. J Biol Chem 279(52):54117-23. [PubMed: 15471863] [MGI Ref ID J:95161]
Voehringer D; Stanley SA; Cox JS; Completo GC; Lowary TL; Locksley RM. 2007. Nippostrongylus brasiliensis: identification of intelectin-1 and -2 as Stat6-dependent genes expressed in lung and intestine during infection. Exp Parasitol 116(4):458-66. [PubMed: 17420014] [MGI Ref ID J:128858]
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Wang Y; Souabni A; Flavell RA; Wan YY. 2010. An intrinsic mechanism predisposes foxp3-expressing regulatory T cells to th2 conversion in vivo. J Immunol 185(10):5983-92. [PubMed: 20944002] [MGI Ref ID J:165629]
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Wiethe C; Debus A; Mohrs M; Steinkasserer A; Lutz M; Gessner A. 2008. Dendritic Cell Differentiation State and Their Interaction with NKT Cells Determine Th1/Th2 Differentiation in the Murine Model of Leishmania major Infection. J Immunol 180(7):4371-81. [PubMed: 18354157] [MGI Ref ID J:132974]
Williams CL; Schilling MM; Cho SH; Lee K; Wei M; Aditi; Boothby M. 2013. STAT4 and T-bet are required for the plasticity of IFN-gamma expression across Th2 ontogeny and influence changes in Ifng promoter DNA methylation. J Immunol 191(2):678-87. [PubMed: 23761633] [MGI Ref ID J:204820]
Wilson EH; Zaph C; Mohrs M; Welcher A; Siu J; Artis D; Hunter CA. 2006. B7RP-1-ICOS interactions are required for optimal infection-induced expansion of CD4+ Th1 and Th2 responses. J Immunol 177(4):2365-72. [PubMed: 16887998] [MGI Ref ID J:138389]
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Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Both heterozygous and homozygous mice are reported to be viable and fertile.
Pricing for USA, Canada and Mexico shipping destinations |
Cryopreserved Mice - Ready for Recovery
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Price (US dollars $) Cryorecovery* $2140.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
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Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Cryopreserved Mice - Ready for Recovery
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Price (US dollars $) Cryorecovery* $2782.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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text:
John T. Moore
Position: Small Forward ▪ Shoots: Right
Height: 6-5 ▪ Weight: 180 lbs.
Born: September 24, 1932
High School: Overbrook in Philadelphia, Pennsylvania
College: La Salle University
NBA Debut: October 31, 1954
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Register | FAQ | Blowout Cards Shop | My iTrader | Forum Rules | Members List | Search | Today's Posts | Mark Forums Read |
Off Topic This section may contain threads that are NSFW. This section is given a bit of leeway on some of the rules and so you may see some mild language and even some risqué images. Please no threads about race, religion, politics, or sexual orientation. Please no self promotion, sign up, or fundraising threads. |
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04-10-2013, 01:59 PM | #164 (permalink) |
Day 2 - just starting up again lets do this
Collecting: Jets players, Raul Mondesi, Ross Gload, Mariekson Gregorious then whatever catches my eye. Just starting a photobucket,checkoutmycards. Always up for trades, just give a PM! RIP PAPAJIM |
04-10-2013, 02:33 PM | #165 (permalink) |
Join Date: Feb 2009
Location: North Mexico
Posts: 15,598
Day 5. Time to take a dump.
Looking for A&G sized minis and Topps Olympic relics, pins, etc. for my daughter and refractors for my son Never trust a man who dresses up like a hot dog. Don't drink and break. It's the law. |
04-10-2013, 04:53 PM | #168 (permalink) |
Day 2. Spent last night in an ER had about 2 hours of sleep. Everything is fine but I feel like I hung out in purgatory for 12 hours.
#Team Connor Running For Connor - http://www.blowoutcards.com/forums/off-topic/556420-running-connor.html |
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text:
DALLAS – Wyoming Valley West's Evan Pirillo and Dallas' Ryan Georgetti shared medalist honors each carding 38 on Monday at Irem Golf Club, but the final three scorers for the Spartans fared better in a 159-170 victory in a Wyoming Valley Conference golf match.
(at Irem G.C. par-36)
WVW: Evan Pirillo 38, Colin Harrison 39, Chris Nixon 40, Leanne Dellarte 42
DAL: Ryan Georgetti 38, Chad DeBona 43, Justin Brojakowski 44, Brendan Balogh 45
The Warriors pulled out the 20-stroke victory led by Zack Mulhern taking medalist firing 36.
(at Fox Hill C.C. par-35)
LL: Nick Egan 42, Mike Murphy 46, Robert Ide 47, Ben Pilch 53
WA: Zack Mulhern 36, Courtney Melvin 43, Colin Herron 43, Madeline Wharton 46
All five scorers for the Royals shot 38 or better as the team cruised to a win over Nanticoke.
(at Lehman G.C. par-34)
HR: Matt Slavoski 33, Chase Makowski 33, Mariano Medico 35, Ryan Crossin 38, Mike Boland 38
NAN: Ricky Ultsh 41, Mike Malshefski 42, Anthony Seiwell 43, Justin Lewis 45
Race Sick medaled with a 41 for Tunkhannock in defeat of Hazleton Area in a match played September 6th.
(at Sugarloaf G.C. par-36)
TUN: Race Sick 41, Sean Soltysiak 44, Jim Lymons 44, Brent Christ 48
HAZ: Rich Gawel 46, Dave Mehalick 50, Nick Thrash 50, Josh Provost 50
Rudy and Ryan Georgetti each shot a 40 for Dallas in a defeat of Hazleton Area from a match played August 30th.
(at Irem G.C. par-36)
DAL: Rudy Georgetti 40, Ryan Georgetti 40, Justin Brojakowski 44, Chad Debona 44
HAZ: Rich Gawel 40, Dave Mehalick 48, Josh Provost 49, Joe Baran 51


text:
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Reflecting on Reflections - June 2014
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Audibility of a CD-Standard A/DA/A Loop Inserted into High-Resolution Audio Playback - September 2007
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AES E-Library
Software Application for Electroacoustic Measurements Using the Time Delay Spectrometry (TDS) Method
The current paper presents a software application that conducts electroacoustic measurements using a digital approach to Time Delay Spectrometry. Development was focused on simplified hardware requirements such as a personal desktop or laptop computer. A friendly and flexible user interface has been designed. Linear and logarithmic sweep test signals are generated and reproduced. System under test (e.g. room) response is recorded and stored in the hard disk. Energy Time Curve (ETC) and Frequency domain analysis procedures are guided efficiently. Reverberation time in the case of a room is estimated very fast. All task data may be restored later for further analysis. Finally, the results of comparison measurements using our application to measurements using a widely accepted TDS analyzer are presented
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Brookfield, Vermont
Brookfield is a town in Orange County, in the "Eastern Vermont Gateway" region.
Brookfield is best known for its floating bridge which spans Sunset Lake buoyed by pontoons. It's the only floating bridge east of the Mississippi River. As of September 2010, the floating bridge was closed for repairs until the town is able to raise money to fix it.
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Region 8: Eastern Vermont Gateway | Northfield | Williamstown | Chelsea | Tunbridge | Randolph | Braintree | Roxbury
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1. Pope forces resignation of Toowoomba Bishop; 2. A complete idiot's guide to Buddhism?
by Noel Debien with Buddhist practitioners
1. Breaking news - Pope forces resignation of William Morris, Catholic Bishop of Toowoomba - (interview with Bishop Geoffrey Robinson, former auxiliary bishop of Sydney). Some background to the story can be found in the Australian catholic clergy publication The Swag
2. Buddhism: Ever been told to wake up to yourself? In a much, much more polite way, that's what Buddhism is really all about- waking up. Roughly 300 million people follow the Buddha's path, and its popularity in the West continues to rise. The Buddha's birthday falls in this month - and we're talking about some of the basics of Buddhism. It's the fastest growing religion in Australia by percentage- and there are now nearly half a million Buddhist in here. Some are ethnic-Buddhist - migrants from places like South East Asia and Sri Lanka- but many are Anglo-Saxons and anglo-Celt converts to this Eastern religion. The Tibetan Buddhism of the Dalai Lama has been particularly successful at attracting Aussie converts - but so have other forms of Buddhism.
Bhante Tejadhammo works with the Engaged Buddhists- a group including many forms of Buddhism. He's a Theravadin - also called the "teaching of the elders". It is a very old and early form of Buddhism. Theravada is seen most obviously in its monasteries in Thailand, Laos and Kampuchea.
Gillian Coote, is the co-ordinating teacher of the Sydney Zen Centre
Gary Gach is an American author, translator, editor, teacher and poet. In 2004, he was appointed to serve on the International Advisory Panel of the Buddhist Channel, a Malaysian Buddhist news website. Gary teaches mindfulness in the tradition of Ven. Thich Nhat Hanh, at the Church for the Fellowship of All Peoples. He is the author of "The complete idiot's guide to Buddhism". His summary of core Buddhist teachings are below.
The Three Jewels
1. Buddha (The Awakened One)
2. Dharma (Buddha's teachings and all they pertain to)
3. Sangha (the practice, and the community of practice)
The Four Noble Truths
1. Life entails hurt, needless suffering (dukkha).
2. Needless suffering results from grasping, craving an illusion (trishna).
3. There's an end to needless suffering (nirvana).
4. The way to the end of needless suffering is the Path (maggha).
The Path
1. Right view
2. Right thought
3. Right speech
4. Right action
5. Right livelihood
6. Right effort
7. Right mindfulness
8. Right concentration
The Precepts
1. Not killing
2. Not stealing
3. Not lying
4. No intoxicants
5. No sexual abuse
You are responsible for your thoughts, words, and actions.
Each results in a reciprocal thought, word, or action.


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Nickname: | Rachel |
User Activity: | 25 pts total (0 pts in March) |
Date of Birth: | 12/21/1988 |
Country: | United states |
Gender: | Female |
Language: | English |
About: | I try to be as open as I can about the world around me. I've picked up tidbits of wisdom from both predictable and unlikely sources, and I aspire to share what I have learned with many people. I want to encourage others to go out, have new experiences, and grow as people. I'm completely over-idealistic, but sometimes, that's what this world needs. |
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Harry Potter
Write an Article
Harry Potter Opinion Article
The Top Harry Potter Inspirational Quotes
My opinion of the top inspirational quotes for the Harry Potter Series. Comment on anything I missed!
-It does not do to dwell on dreams and forget to live -There are all kinds of courage. It takes a great deal of courage to stand up to our enemies, but a great deal more to stand up to our friends -Do not pity the dead, Harry. Pity the living, and, above all, those who live without love. -If you want to know what a man's like take a look at how he treats his inferiors not his equals -It is out choices that show who we truly are, far more than our abilities. -Happiness can be found even in the darkest of times, if one remembers to turn on the light. - You fail to recognize that it matters not what someone is born, but what they grow to be -There was no point in worrying yet.... what would come, would come... and he would have to meet it when it did. -Of course it is happening inside your head, but why on earth should that mean that it is not real? -Soon we must all face the choice between what is right and what is easy. -The truth is a beautiful and terrible thing, and should therefore be treated with caution. -As much money and life as you could want! The two things most human beings would choose above all - the trouble is, humans do have a knack of choosing precisely those things that are worst for them. -Numbing the pain for a while will make it worse when you finally feel it. -People find it far easier to forgive others for being wrong than being right. -Differences of habit and language are nothing at all if our aims are identical and our hearts are open. |
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28 comments
totalpotterfan said:
I'm going to bed before any of you find another way to get us killed or worse expelled! she needs to sort out her priorities!
posted over a year ago.
evil_genius said:
i think most of them were said by Dumbledore (the great)...
posted over a year ago.
ravenclaw8814 said:
"Even though we come from different places and speak in different tongues, our hearts beat as one," -Albus Dumbledore.
That's my favorite quote
posted over a year ago.
grayfish03 said:
"I want you to listen to me very carefully, Harry. You're not a bad person. You're a very good person, who bad things have happened to. Besides, the world isn't split into good people and Death Eaters. We've all got both light and dark inside us. What matters is the part we choose to act on. That's who we really are." -Sirus Black
It's from the movie, not the book, but it's one of my faves!
posted over a year ago.
Christine0223 said:
I don't go looking for trouble, trouble usually finds me. - Harry
posted over a year ago.
Flora_Swift said:
"All is fair in love and war, and this is a bit of both."-Ron
posted over a year ago.
teacupitty43 said:
hey guys please attend the poll http://www.fanpop.com/clubs/harry-potteer/
posted over a year ago.
rhythmicmagic said:
"It was important, Dumbledore said, to fight, and fight again, and keep fighting, for only then could evil be kept at bay, though never quite eradicated."
posted over a year ago.
georgia_joan said:
The things we lose always Show up In the end. Even In ways We Don't Always Expect - Luna Lovegood
posted over a year ago.
thaliagranger said:
'' Turn to page 394 '' - Severus Snape, The half blood prince ;)
posted over a year ago.
02katy19 said:
it's are choices who decides who we are not are abilities. Dumbledore chamber of secrets
posted over a year ago.
TeutonicMight said:
"Nitwit! Blubber! Oddment! Tweak!" You missed what was quite possibly the most inspirational quote of all...kidding, kidding. Wonderful article, though. 8'D
posted over a year ago.
antonpotter said:
"Help will always be given at Hogwarts to those who ask for it." Dumbledore
posted over a year ago.
Flora_Swift said:
It doesn't matter when someone is born, it matters what they grow up to be. - Dumbledore
posted over a year ago.
aquagirl445 said:
Dumbledore: After all this time?
Severus: Always Love it, love it, love it!
posted over a year ago.
Snowgirl529 said:
"The one that love us, never truly leave us. You can always find them in here <3" - Sirius Black
posted over a year ago.
Dancin4evah said:
One of my favorites : "I'll join you when hell freezes over! Dumbledore's Army!"
-- Neville Longbottom to Lord Voldemort in the Deathly Hallows. No, I don't know whether this is actually inspirational or not, I just consider it one of Neville's finest moments.
posted over a year ago.
FanFic_Girl_26 said:
I like how Dumbledore always has such wise things to say, especially to Harry and his friends, too. :) Sometimes he would reveal information about his family, which is shown in Harry Potter and the Goblet of Fire:
"My own brother, Aberforth, was prosecuted for practicing inappropriate charms on a goat. It was all over the papers, but did Aberforth hide? No, he did not! He held his head high and went about his business as usual! Of course, I'm not entirely sure he can read, so that may not have been bravery. . . ."
posted over a year ago.
ElizaSkywalker said:
It is our choices that show who we are, far more than our abilities
posted over a year ago.
hermionicole said:
"It's going to be all right, sir," Harry said over and over again, more worried by Dumbledore's silence than he had been by his weakened voice. "We're nearly there ... I can Apparate us both back ... don't worry ..."
"I am not worried, Harry," said Dumbledore, his voice a little stronger despite the freezing water. "I am with you."
posted 5 months ago.
Luinnen said:
"We have something Voldemort doesen't have."
"What is it?" "Something worth fighting for." -Harry Potter
posted 4 months ago.
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