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A vast amount of data suggests that homologous recombination in mammalian cells is relatively rare as compared to random integration, imposing the need for sophisticated selection protocols to enrich for cells in which homologous recombination has occurred. We here show that one of the key factors in efficient homologous recombination is the use of isogenic DNA to prepare the targeting vectors. Homologous recombination at the retinoblastoma susceptibility gene (Rb) in embryonic stem cells derived from mouse strain 129 was 20-fold more efficient with a 129-derived targeting construct than with a BALB/c-derived construct. The two constructs were identical, except for a number of base sequence divergences between 129 and BALB/c DNA, including base-pair substitutions, small deletions/insertions, and a polymorphic CA repeat. Transfection with an isogenic DNA construct, containing 17 kilobases of homology, yielded a targeting frequency of 78% (of a total of 20,000 drug-resistant colonies), without the use of an enrichment protocol for homologous recombination. This result indicates that, also in mammalian cells, homologous recombination rather than random integration can be the predominant event.
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For now, no Web-site operator really knows what her true traffic is. Neither the panel nor the census system is perfect, but by going back and forth between the two, publishers and advertisers can make relative comparisons within their market. That's how the industry has continued to push forward.
"Sites do come to us with their own internal information, but we are still going to use the syndicated [panel] tools available," says Julian Zilberbrand, the associate director of digital ad operations at MediaVest, a major online advertising buyer. "The truth will lie somewhere in between."
But there is some hope for reconciliation between the two standards of counting traffic.
That's where George Ivie, executive director and CEO of the Media Ratings Council (MRC), comes in. The council is a not-for-profit trade association formed in the wake of the 1960 Harris Committee Hearings. The committee investigated the business practices of radio and television and concluded that the broadcast media would self-regulate, including performing independent audits to determine the size of its audience. The ratings council was created to set standards for measuring broadcast audiences and to accredit organizations, like Nielsen, that measure those audiences. "We haven't changed that much since the 1960s in that we have a focused mission—to try to improve for the industry the quality of media measurements," says Ivie.
In 2002, the council turned its attention to the Internet, and has found it to be a different kind of beast entirely than TV and radio. The first thing that it did was write standards for audience census data, which include page views, clicks on a page, and time spent on a site—all the new types of audience measurement born with the Internet.
News organizations that want their traffic counts to be accredited by the ratings council must first adopt the council's standards, which include, for example, counting only content that is accessed through an end browser. (Some organizations would count URLs sent through e-mail as a page impression, without actually verifying that the site was ever visited through a browser.)
To date, the council's standards have been agreed to only by its 108 affiliate news and advertising organizations. And only a handful of these affiliate organizations—including Yahoo, msn, Weather.com, Univision, AOL, and advertisers like Atlas and DoubleClick—have been audited by the MRC, giving these operations an advantage when negotiating advertising rates.
More important, though, the MRC began auditing comScore and Nielsen/ NetRatings in 2006, a five-phase process that won't be complete until later this year. Ivie says this will allow a reconciliation between panel- and census-based traffic estimates. "When we are done with the auditing, I'll tell you how I feel about their random-digit panels," says Ivie. "How do we know they are representative of the U.S.? Right now, I don't."
One concern, however, is that before the auditing of comScore and Nielsen/ NetRatings is complete, the volatile online world will produce a new silver bullet in traffic metrics.
Last July, for instance, Nielsen/Net-Ratings decided that time spent on a site was more important than total Web page views, an acknowledgment of changing online habits that include watching video (which can keep readers on a site longer) and Ajax, computer coding that allows a visitor to interact with a Web page—voting, tagging, or moving parts of the page around—without having to reload an entirely new page. At the same time, having a window open on a site for hours doesn't mean the visitor is "engaged" the whole time—he could have simply gone to get a cup of coffee—which makes "time spent" an incredibly tough metric to measure accurately.
In a few years there could be an entirely new way to describe how news organizations measure their online relevance. If that does turn out to be the case, the Media Ratings Council and the news industry are, once again, going to have more catching up to do. But that's the nature of an evolving medium whose future is unpredictable.

subset: commoncrawl


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In our new release, it's even easier to get started with your favorite OSS application from the App Gallery.
The Quick Start screen has new features, but we've slimmed it down to show only what's necessary when WebMatrix first loads. Start here whether you want a template, OSS app, remote or existing site.
The new breadcrumb bar across the top displays the stages required to install an app, and tracks which ones you've completed as you go.
WebMatrix can store your database root password for later use. Entering it once saves you from having to enter it again later when you install other apps.
On the EULA page, you will notice the new, simpler Direct Download Link that takes you to the download for the application you selected.
WebMatrix now handles many application parameters, like the database name, username and password, for you, simplifying your installation experience. The Copy to Clipboard link allows you to save the information for later use.
If you want to learn more about other great features in WebMatrix 2 Beta, check out some of these articles:


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4 Reasons Obama's Second Term May Be Different
Election Day 2012 delivered three major outcomes:
- Barack Obama won another term as president.
- Republicans retained control of the House of Representatives.
- The Democratic/Independent coalition retained control of the Senate.
In other words, voters signed up for the same general conditions they had before the election. So why might things be any different for Americans' finances over the next four years?
Reasons change may be near
It seems doubtful that votes to keep the White House and Congress in the same hands were resounding endorsements of the status quo. Americans face pressing economic problems, including persistently high unemployment and a looming deficit crisis. But here are four reasons why the years ahead may be different:
- The uncertainty is resolved. Business managers hate uncertainty. Forget any myths about whether Republicans or Democrats are inherently better for business conditions. Since the end of World War II, the economy has performed nearly identically under Republican and Democratic administrations. Annual inflation-adjusted GDP has averaged 2.9 percent under Republicans, and 3.0 percent under Democrats. The bottom line is that smart businesses will adjust to whatever rules are in place -- they just want to know what the rules are going to be.
- Obama can't run again. Right from Obama's inauguration, a goal of Republican Congressional strategy was to try to make him a one-term President by minimizing the number of legislative victories he could claim. By nature, that's a negative, obstructionist approach. Now that Obama has won a second term, there will be no incumbent running in 2016. That means that would-be candidates on both sides of the aisle will want to start burnishing their track records, and that means having some accomplishments to show.
- Recognition is growing that gridlock is not a winning formula. For a long time, there was a notion among fiscal conservatives that gridlock in Washington was actually a good thing, because it prevented new spending measures from being passed. However, the huge expansion in the federal budget due to two wars and the fiscal crisis changed the game. The current course of spending means that active steps need to be taken to rein in the budget.
- A narrow anti-tax platform won narrow support. The Obama campaign was successful in eroding Mitt Romney's perceived advantage on economic issues by defining the key fiscal difference between the two candidates as the willingness to tax earners above $250,000 in order to address the deficit. This was a numbers game that Obama was bound to win. Right or wrong, Romney's defense of the wallets of the wealthiest Americans wasn't going to win him many votes.
Whether things are really different will be easy to tell: If America sails off the fiscal cliff at the end of this year, or otherwise fails to put in place many widely agreed-upon measures to responsibly address the deficit, it will be a sign that it's back to business as usual in Washington.
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text:
April 15, 2014
Six members of John C. Lincoln's medical staffs were honored in early April through John C. Lincoln's annual physician recognition program.
The program was designed to spotlight a wider variety of contributions, accomplishments and service by members of the medical staffs at both Network hospitals and medical practices.
Eligibility for all these awards is based on service to the John C. Lincoln Hospitals and community; excellence in leadership, ethics and individual achievement; and excellence in patient care, customer service and patient satisfaction.
Here are the honorees:
Nelson Faux, MD
Nelson Faux, MD, an emergency medicine and critical care physician, last week received the 2014 President's Award for Lifetime Achievement for his many years of outstanding service within the John C. Lincoln Health Network. He joins distinguished company for the award — pulmonologist David Drachler, MD, received the honor a few years ago.
Also honored were:
2014 Distinguished Medical Service Award:
2014 Rising Physician Award:
Dr. Faux, chief of staff at North Mountain from 2002 to 2005 and from 2006 to 2009 has served on numerous committees at both hospitals, including more than 15 committees currently. He joined the North Mountain medical staff in 1978 and served as a member of the John C. Lincoln Health Network Board of Directors from 2002-2011. He also has been instrumental in the Arizona EMT and Air Evac program since 1980.
Maggi Griffin and John Harrington, North Mountain and Deer Valley CEOs, recognized Dr. Faux at their award ceremonies for his long record of service and contributions to the organization. "The Health Network has benefited greatly from his work," Maggi said.
Dr. Ryan, board certified by the American Board of Internal Medicine (ABIM) and ABIM Geriatrics, "is very compassionate and always takes time to listen to staff and his patients," his nominator said. "He has always amazed me with his detailed assessments and excellent/caring bedside manner. He is always respectful to staff and takes the time and energy to answer questions about patients' care or the physiology of a disease process."
Dr. Peralta, board certified by the American Board of Internal Medicine, "strives to provide optimal care to his patients," his nominator said. "His involvement with the professional review committee has shown me that he cares about patients and that others deliver optimal care while practicing at John C. Lincoln. We love him on the Ortho floor because he is very communicative, friendly and compassionate."
Dr. Whitaker, is board certified by the American Osteopathic Board of Family Physicians. "He is an outstanding family physician who is recognized in the community for his commitment to patient-centered medical care. He always has time to communicate with his patients, calls/texts them on weekends and at night and consistently goes the extra mile to ensure high-quality patient care. He is also highly esteemed by his colleagues and co-workers," his nominator said.
Dr. Mangram, board certified by the American Board of Surgery (ABS) and by ABS Surgical Critical Care, "works tirelessly in her pursuit of helping the trauma service be the best in the Phoenix area," said her nominator. "An outstanding example of her skills in communication, collaboration and teamwork is her renowned G-60 program for geriatric trauma patients. She brings respect, discipline, daily learning, the desire to work hard, compassion for patients and families, the importance of caring for each other, and an infectious positive attitude. She is truly a rare gem. We are so lucky to have her as the leader of the trauma program."
Dr. Khera is board certified by the American Board of Orthopedic Surgery. "His patients always give him very good reviews and feel great about their plan of care because he takes the time to explain it to them and to receive their input as well," said his nominator. "He is a great listener, not only to the patients, but also to the nurses. He's never too hard to reach when the nurses may need something for his patients."
Return to main News page.
© Copyright John C. Lincoln Health Network (Phoenix, Arizona)


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Knight Blades Larp the Underworld Campaign
By Jenny Rota & Jorge Paiz
Jorge Paiz Larp Noob:
This past weekend I Jorge Paiz, went to my first ever LARP, (Live Action Role Play) the larp I attended was called Knight Blades. In Knight Blades new players pick what kind of character they want to by choosing a race a and class. Such races and classes include Dwarfs, Orcs, High elves, Silver elves,and Humans. There are also many classes such as Clerics, Mages, Warriors, and Rogues. In Knight Blades you can make your own unique character such as a High elf Rogue, Orc Warrior, Human Mage. etc. As well as your own unique name. now creating your own character is a tip of the Ice berg. What comes next is what helps you get through the game and that's combat!
In a larp combat one usually has many ways to enter in well armed. One thing that most larp players use is a boffer sword, which a is foam sword used for larping. You also can use latex weapons as long as they are well padded and have two inches of foam at the tip for thrusting. Also if you are a spell and magic user you use things called spell packets or spell components in game that are made of 7 by 7 inch square fabric and a table spoon bird seed. What you do with with these packets is say the incantation depending on your class and throw them at your enemy. Some spells cause damage some stall and hinder your enemies.
What I really enjoyed about Knight Blades was entering the town of Stormhammer. The town of of Stormhammer is like entering a whole new world with many different unique characters as if you were in an actual MMORPG Stormhammer resides on the boarders of the Kingdom of Boronia which is ruled by the Good King Henry XXI. The town has many guilds and company's in which players can be a part of to gain professions to earn gold, magic abilities and Items. When your in Stormhammer you are no longer in a world where cell phone, internet and laptops exist, you are in a realm were you are literally back in time and you must role play as if you are in that time period. Your going off on quests, fighting monsters, trolls, ogres, goblins, giants, and even dragons. You get given assignments by leaders of company's you join as well as the mayor and the king him self. This being my first Larp as my character Kazarneth was quiet thrilling I enjoyed being a high elf earth mage and combating many enemies. i also enjoyed the experience of being part of the mage guild and the company known as "The Company of The Silver Dragons" I enjoyed protecting both king and country with them in the game. Also a bit of advice if your a noob going into knight Blades or any sort of larp a bit of advice drink a lot of water between battles and stay alive.
Jenny Rota Larp Veteran;
I Jenny Rota also attended Knight Blades this weekend. i have been playing knightblades for just over a year now. I play as a Silver Elf fire mage. I was the one who dragged Jorge here into larping. "The Company of the Silver Dragons" is a very nice group of players who helped me out when i was a noob to larping. and now I am currently an officer in the guild. this means I can tell Jorge what to do. and also provide some guidance on combat.
There are a few things that you should really know before you enter any larp as a player. One of the most important things is the monsters that we all fight are also human beings behind all their masks, makeup and other costuming. You don't want to hit them hard enough with your sword to the point where it causes actual pain to the NPC. It is very important that you respect the NPCs just like you respect the other players in game. you could potentially cause enough pain to the monster that they would not be allowed to role play as other monsters to kill. Besides, the more monsters to kill the more fun your larping experience will be. Another thing you should be aware of is the set of rules that both the players and npcs fallow. there are certain lists of abilities that everyone should know what they do. for example, in Knight Blades there is a skill called "Waylay" that a lot of the players have. When used properly, the ability knocks the target unconscious for five minutes. Also know that every larp has its own set of rules and regulations in regards to armor, weapons and the play style of the game. it is very important that you familiarize yourself with the rules before you enter the game. One last tip I offer is know what your character can do ability wise before you enter the game. if you are a spell caster such as a mage or cleric, know your spell incants. These are short lines that you say to tell people around you what you are casting, and how much damage the target will take if you hit them with the spell packet. It is crucial that you say the incant right. If you do not you waste the mana even though the spell was not successfully cast. if you are a fighter class it is just as important to know what your character does and now much health you have when you start off.
This past Knight Blades event many exciting things have happened. one of the most eventful things that happened was we finally sealed all the 7 deadly sins back into their realms where they will remain forever. Also a fallen dark god known as the Ravenger is trying to take power once more. He is currently trying to take the place of another god, putting the grey god Sepsis out of power. In the land of Baronia there are many gods that are worshiped. you can worship gods of the light, dark, or grey which are the neutral gods. there are a lot of mixed feeling with the Ravenger coming to power which is causing tension within the people of Stormhammer. A little advice for new players, keep which god you worship to yourself. Only tell those you trust which deity you fallow if you wish to.
For the next event I am expecting most of our combat to be against followers of the Ravenger, and possibly the Ravenger himself. I also expect followers of Sepsis to be out as well. Whatever we face I am sure that it will be a blast and I am looking forward to next event.
For more information about Knight Blades, click the link at the top of the article or the link at the bottom.
Photos by Sundance Wolf


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Stock Futures Point to Rebound
TheStreet) -- Stock futures were pointing to a financials-supported rebound Friday, after the previous session's deep selloff, as investors shrugged off Moody's downgrade of the major banks.
Futures for the
Dow Jones Industrial Average were gaining 44 points, or 45.4 points above fair value, at 12,546. Futures for the
S&P 500 were rising by 5 points, or 4.1 points above fair value, at 1323, and futures for the
Nasdaq 100 were ahead by 5.5 points, or 6.1 points above fair value, at 2557.
Stocks suffered their worst single-day drop in three weeks Thursday as investors were spooked by a gloomy batch of global economic data, a bearish trading call by Goldman Sachs and disappointment with the outcome of the Federal Reserve's latest policy meeting.
On Thursday evening, Moody's downgraded fifteen of the biggest global banks, but they followed with a rally.
Citigroup (C), Bank of America (BAC) and Morgan Stanley (MS) were among the banks who saw their credit ratings slashed as expected, and they were rising by a respective 1.1%, 1.5% and 2.6% in premarket trading Friday. The Hong Kong Hang Seng index closed lower by 1.4% and the Nikkei in Japan closed down 0.29%. The FTSE in London was slipping by 0.75% and the DAX in Germany was declining by 0.53% as business confidence in the biggest eurozone economy fell to its lowest point in more than two years. European leaders are meeting in Rome Friday to discuss a unanimous plan on bringing relief to the eurozone debt crisis. One area of contention going into the meetings is the proposal to tax financial transactions and to have banks foot the bill for their own bailouts. In Spain, independent audits indicate that the country's banks will, under duress, require up to €62 billion in financial support, below the earlier foreseen €100 billion in eurozone bailouts. Friday's U.S. economic calendar is empty. August crude oil futures were edging up 53 cents to $78.73 a barrel. August gold futures were rising $4.70 to $1,570.20 an ounce. The benchmark 10-year Treasury was lower by 1/32, raising the yield to 1.622%, while the dollar was flat, according to the dollar index. In corporate news, shares of Darden Restaurants (DRI) fell in pre-market action after the Orlando-based company posted in-line earnings for its fiscal fourth quarter but came in slightly short on the top line. Arch Coal (ACI) said after Thursday's closing bell that it's laying off 750 employees, or roughly 10% of its workforce. The company said it plans to "idle several operations and to reduce production at other mining complexes in Appalachia due to the unprecedented downturn in demand for coal-based electricity. " Medtronic (MDT) said its board has approved a 7.2% increase in its regular quarterly cash dividend to 26 cents a share. The medical device company said the dividend is payable on July 27 to shareholders of record on July 6. Monster Beverage (MNST - Get Report) is being added to the S&P 500, replacing Sara Lee (SLE). Standard & Poor's expects the change to take place after the closing bell on June 28. The change was prompted by Sara Lee's decision to spin off its international coffee & tea business to shareholders. Ryder Systems (R - Get Report) lowered its fiscal second-quarter outlook, citing weakness in its fleet management solutions business. The company now sees comparable earnings of 90 to 95 cents a share for the three months ended in June, down from a previous expectation for a profit of $1.07 to $1.12 a share.
2012 Stock Predictions and Outlook
Your one-stop shop for 2012 stock recommendations and market predictions. |
>To contact the writer of this article, click here: Andrea Tse.
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