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Publications (78)248.82 Total impact
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ABSTRACT: The generation of a wide ESTs library and database from Trichoderma harzianum CECT 2413 was the base for identifying the gene ThPTR2, coding for a PTR family di/tri-peptide transporter. The deduced protein sequence of the ThPTR2 gene showed the conserved motifs and also the 12 transmembrane domains typical of the PTR transporters. The highest level of ThPTR2 expression was found when the fungus was grown in chitin as sole carbon source. We also found that ThPTR2 expression was increased when Trichoderma interacted directly in solid medium with the plant-pathogenic fungus Botrytis cinerea, showing that ThPTR2 is involved in the mycoparasitic process. Additionally, its expression was triggered by nitrogen starvation and a higher level of expression was also found when Trichoderma was grown in secondary nitrogen sources like allantoin, yeast extract, and urea. However, no difference was found when Trichoderma was grown in presence or absence of glucose as carbon source. Strain T34-15, a transformant that overexpressed the ThPTR2 gene, showed about a 2-fold increase in the uptake of the dipeptide Leu-Leu. Additionally, two transformants from the strain Trichoderma longibrachiatum T52 that overexpressed ThPTR2 were also studied, confirming the role of this gene in peptide transport. Other homologous genes to ThPTR2 were identified in other Trichoderma strains. ThPTR2 is the first experimentally confirmed PTR family transporter gene from filamentous fungi.Fungal Genetics and Biology 05/2006; 43(4):234-46. · 3.26 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Different tissues of potato, tobacco, and bean plants were screened for anti-fungal protease inhibitor (PI) activity, also following fungal pathogen inoculation or mechanical wounding. A potato (Solanum tuberosum var. Desireé) sprout protein extract showed a strong inhibitory activity against chymotrypsin and Botrytis cinerea fungal proteases, but also on spore germination, hyphal elongation, and development of necrotic lesions. An active mixture of different proteins was affinity column purified and sequenced. Two new anti-fungal genes, PKI1 and PPI3B2, coding, respectively, for a Kunitz-type inhibitor and a Proteinase Inhibitor 1 capable of reducing fungal lesion development, were cloned and partially characterized. Direct effect on leaf necrosis formation was found to be dependent on the anti-chymotrypsin activity of both selected inhibitors. The PKI1 transcript was found to accumulate in untreated sprout tissues, although homologues of this gene seemed to accumulate following Bemisia tabaci attack. In the case of PPI3B2, we provide preliminary evidence that a member of the Proteinase Inhibitor 1 family is active against not only herbivorous insects but also phytopathogenic fungi and foliar lesions caused by them.Physiological and Molecular Plant Pathology 04/2006; · 1.98 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Trichoderma species are commonly used as biocontrol agents of different plant-pathogenic fungi. Terpene compounds are involved in the biocontrol process due to their antifungal properties (e.g., ergokonins and viridins) but additionally their structural function in the cell membranes (ergosterol) is essential. We report here the characterization of the T. harzianum erg1 gene, encoding a squalene epoxidase, a key enzyme in the biosynthesis of triterpene derivatives such as ergosterol. In T. harzianum the partial silencing of the erg1 gene gave rise to transformants with a higher level of sensitivity to terbinafine, an antifungal compound that acts specifically over the squalene epoxidase activity. In addition, these silenced transformants produced lower levels of ergosterol than the wild type strain. Finally, the silencing of the erg1 gene resulted in an increase in the expression level of the erg7 gene that encodes the oxidosqualene lanosterol-cyclase, another enzyme of the terpene biosynthesis pathway.Fungal Genetics and Biology 04/2006; 43(3):164-78. · 3.26 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The entomopathogenic anamorphic fungus Metarhizum anisopliae is currently used as a biocontrol agent (BCA) of insects. In the present work, we analyzed the sequence data obtained from group I introns in the large subunit (LSU) of rDNA genes with a view to determining the genetic diversity present in an autochthonous collection of twenty-six M. anisopliae isolates selected as BCAs. DNA fragments corresponding to the 3'-end of the nuclear LSU rDNA genes of 26 M. anisopliae isolates were amplified by PCR. The amplicon sizes ranged from 0.8 to 3.4-kb. Four intron insertion sites, according to Escherichia coli J01695 numbering, were detected--Ec1921, Ec2066, Ec2449 and Ec2563--after sequencing and analysis of the PCR products. The presence/absence of introns allowed the 26 isolates to be distributed into seven genotypes. Nine of the isolates tested showed no introns, 4 had only one, 3 two, and 10 displayed three introns. The most frequent insertion sites were Ec1921 and Ec2449. Of the 26 isolates, 11 showed insertions at Ec2563 and a 1754-bp sequence was observed in ten of them. The most-parsimonious (MP) tree obtained from parsimony analysis of the introns revealed a main set containing four-groups that corresponded to the four insertion sites. Four insertion sites of group I introns in the LSU rDNA genes allowed the establishment of seven genotypes among the twenty-six biocontrol isolates of M. anisopliae. Intron insertions at the Ec2563 site were observed for first time in this species.BMC Microbiology 02/2006; 6:77. · 2.98 Impact FactorThis article is viewable in ResearchGate's enriched format - [Show abstract] [Hide abstract]
ABSTRACT: The characterization of 11- and 18-residue peptaibols (peptides synthesized by peptide synthetases) at Trichoderma harzianum CECT 2413 (a filamentous fungus) was performed. Using a heterologous probe from tex1, the only peptaibol synthetase cloned and characterized so far in Trichoderma species, was cloned; a region that comprised 11676 bp of a second peptide synthetase gene detected in these strain (called salps2) and sequenced. The deduced sequence of Salps2 (3891 amino acids) contained three complete and a fourth incomplete module of a peptide synthetase, in which the typical adenylation, thiolation and condensation domains were found, but also an additional dehydrogenase/reductase domain in the C-terminus of the last module. Based on sequence similarity and analysis of its modular structure, it is proposed that Salps2 is a peptaibol synthetase. Additionally, analysis of =4.4-kb sequence downstream of salps2 was done and the signature sequences of Salps2 were identified and compared with those of available sequences of the other Trichoderma peptaibol synthetases.Folia Microbiologica 02/2006; 51(2):114-20. · 1.15 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The filamentous fungus Trichoderma harzianum is used as biological control agent of several plant-pathogenic fungi. In order to study the genome of this fungus, a functional genomics project called "TrichoEST" was developed to give insights into genes involved in biological control activities using an approach based on the generation of expressed sequence tags (ESTs). Eight different cDNA libraries from T. harzianum strain CECT 2413 were constructed. Different growth conditions involving mainly different nutrient conditions and/or stresses were used. We here present the analysis of the 8,710 ESTs generated. A total of 3,478 unique sequences were identified of which 81.4% had sequence similarity with GenBank entries, using the BLASTX algorithm. Using the Gene Ontology hierarchy, we performed the annotation of 51.1% of the unique sequences and compared its distribution among the gene libraries. Additionally, the InterProScan algorithm was used in order to further characterize the sequences. The identification of the putatively secreted proteins was also carried out. Later, based on the EST abundance, we examined the highly expressed genes and a hydrophobin was identified as the gene expressed at the highest level. We compared our collection of ESTs with the previous collections obtained from Trichoderma species and we also compared our sequence set with different complete eukaryotic genomes from several animals, plants and fungi. Accordingly, the presence of similar sequences in different kingdoms was also studied. This EST collection and its annotation provide a significant resource for basic and applied research on T. harzianum, a fungus with a high biotechnological interest.BMC Genomics 02/2006; 7:193. · 4.04 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Methanol extracts from 24 Trichoderma isolates, selected as biocontrol agents and representating different species and genotypes from three of the four taxonomic sections of this genus (T. sect. Trichoderma, T. sect. Pachybasium and T. sect. Longibrachiatum) were screened for antibacterial, anti-yeast and antifungal activities against a panel of seven bacteria, seven yeasts and six filamentous fungi previously used in similar studies. Two different growth media were tested (potato dextrose broth and CYS80), and all isolates included in the antimicrobial tests showed at least one inhibitory activity against one of the target microorganisms in one of the two culture media. No statistically significant differences were detected in the number of active strains between the two culture media, but the highest number of inhibitory strains against bacteria and fungi were found in strains from Trichoderma sect. Pachybasium, whereas strains from T. sect. Longibrachiatum showed the highest anti-yeast values. In all cases, a correlation was found between the strains that were active against yeasts and fungi. However, some degree of variability was detected for strains within the same taxonomic section. In general terms, strains from T. asperellum (mainly in CYS80 medium), and T. longibrachiatum gave the best non-enzymatic antimicrobial profiles.Mycological Research 01/2006; 109(Pt 12):1397-406. · 2.81 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A Gram-positive, aerobic, long-rod-shaped, non-spore-forming bacterium (strain PPLB(T)) was isolated from soil mixed with Iberian pig hair. This actinomycete showed keratinase activity in vitro when chicken feathers were added to the culture medium. Strain PPLB(T) was oxidase-negative and catalase-positive and produced lipase and esterase lipase. This actinomycete grew at 40 degrees C on nutrient agar and in the same medium containing 5 % (w/v) NaCl. Growth was observed with many different carbohydrates as the sole carbon source. On the basis of 16S rRNA gene sequence similarity, strain PPLB(T) was shown to belong to the genus Terrabacter of the family Intrasporangiaceae. Strain PPLB(T) showed 98.8 % 16S rRNA gene sequence similarity to Terrabacter tumescens. Chemotaxonomic data, such as the main ubiquinone (MK-8), the main polar lipids (phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol) and the main fatty acids (i-C(15 : 0), ai-C(15 : 0), i-C(16 : 0) and ai-C(17 : 0)) supported the affiliation of strain PPLB(T) to the genus Terrabacter. The G+C content of the DNA was 71 mol%. The results of DNA-DNA hybridization (36.6 % relatedness between Terrabacter tumescens and strain PPLB(T)) and physiological and biochemical tests suggested that strain PPLB(T) belongs to a novel species of the genus Terrabacter, for which the name Terrabacter terrae sp. nov. is proposed. The type strain is PPLB(T) (=CECT 3379T=LMG 22921T).International Journal of Systematic and Evolutionary Microbiology 12/2005; 55(Pt 6):2491-5. · 2.80 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Trichoderma mycoparasitic activity depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. We have analysed the extracellular proteome secreted by T. harzianum CECT 2413 in the presence of different fungal cell walls. Significant differences were detected in 2DE maps, depending on the use of specific cell walls or chitin. A combination of MALDI-TOF and liquid chromatography mass spectrometry allowed the identification of a novel aspartic protease (P6281: MW 33 and pI 4.3) highly induced by fungal cell walls. A broad EST library from T. harzianum CECT 2413 was used to obtain the full-length sequence. The protein showed 44% identity with the polyporopepsin (EC 220.127.116.11) from the basidiomycete Irpex lacteus. Lower identity percentages were found with other pepsin-like proteases from filamentous fungi (<31%) and animals (<29%). Northern blot and promoter sequence analyses support the implication of the protease P6281 in mycoparasitism.Fungal Genetics and Biology 12/2005; 42(11):924-34. · 3.26 Impact Factor -
Article: Thiourea, triazole and thiadiazine compounds and their metal complexes as antifungal agents.
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ABSTRACT: Many currently available antifungal and antibacterial agents have undesirable toxic effects, and a wide spread use of these drugs has lead to rapid development of drug resistant strains which are the leading cause for treatment failure in both clinical and agricultural applications. The present article provides a synopsis of recent progress in investigations of new classes of antifungal compounds: disubstituted aliphatic and aromatic thioureas, triazole and thiazine compounds which act as ligands for transition metals. Antifungal effects of these compounds and selected metallic complexes versus representative plant pathogenic fungi are reviewed.Journal of Inorganic Biochemistry 09/2005; 99(8):1558-72. · 3.27 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A new component of the beta-1,6-glucanase (EC 188.8.131.52) multienzymatic complex secreted by Trichoderma harzianum has been identified and fully characterized. The protein, namely BGN16.3, is the third isozyme displaying endo-beta-1,6-glucanase activity described up to now in T. harzianum CECT 2413. BGN16.3 is an acidic beta-1,6-glucanase that is specifically induced by the presence of fungal cell walls in T. harzianum growth media. The protein was purified to electrophoretical homogenity using its affinity to beta-1,6-glucan as first purification step, followed by chomatofocusing and gel filtration. BGN16.3 has a molecular mass of 46 kDa in SDS/PAGE and a pI of 4.5. The enzyme only showed activity against substrates with beta-1,6-glycosidic linkages, and it has an endohydrolytic mode of action as shown by HPLC analysis of the products of pustulan hydrolysis. The expression profile analysis of BGN16.3 showed a carbon source control of the accumulation of the enzyme, which is fast and strongly induced by fungal cell walls, a condition often regarded as mycoparasitic simulation. The likely involvement beta-1,6-glucanases in this process is discussed.FEBS Journal 08/2005; 272(13):3441-8. · 3.99 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Some of the secondary metabolites produced by Trichoderma, such as the peptaibols and other antibiotics, have a peptide structure and in their biosynthesis are involved proteins belonging to the Non-Ribosomal Peptide Synthetase family. In the present work, a PCR-mediated strategy was used to clone a region corresponding to an adenylation domain of a peptide synthetase (PS) gene from 10 different strains of Trichoderma. In addition, and using the fragment isolated by PCR from T. harzianum CECT 2413 as a probe, a fragment of 19.0 kb corresponding to a PS-encoding gene named salps1, including a 1.5 kb fragment of the promoter, was cloned and sequenced. The cloned region of salps1 contains four complete, and a fifth incomplete, modules, in which are found the adenylation, thiolation and condensation domains, but also an additional epimerization domain at the C-terminal end of the first module. The analysis of the Salps1 protein sequence, taking into consideration published data, suggests that it is neither a peptaibol synthetase nor a protein involved in siderophore biosynthesis. The presence of two breaks in the open reading frame and the expression of this gene under nitrogen starvation conditions suggest that salps1 could be a pseudogene.FEMS Microbiology Letters 04/2005; 244(1):139-48. · 2.72 Impact Factor -
Article: Expression of an alpha-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum.
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ABSTRACT: Trichoderma species have been investigated as biological control agents for over 70 years owing to their ability to antagonize plant pathogenic fungi. Mycoparasitism, one of the main mechanisms involved in the antagonistic activity of Trichoderma strains, depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. The antifungal activity of an alpha-1,3-glucanase (EC 126.96.36.199, enzymes able to degrade alpha-1,3-glucans and also named mutanases) has been described in T. harzianum and its role in mycoparasitic processes has been suggested. In this study, we report on the purification, characterization and cloning of an exo-alpha-1,3-glucanase, namely AGN13.2, from the antagonistic fungus T. asperellum T32. Expression at the transcription level in confrontation assays against the strawberry pathogen Botrytis cinerea strongly supports the role of AGN13.2 during the antagonistic action of T. asperellum.FEBS Journal 02/2005; 272(2):493-9. · 3.99 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Strain identification in situ is an important factor in the monitoring of microorganisms used in the field. In this study, we demonstrated the use of sequence-characterized amplified region (SCAR) markers to detect genomic DNA from Trichoderma harzianum 2413 from soil. Two primers (SCAR A1/SCAR A1c) were tested against DNA of 27 isolates of Trichoderma spp. and amplified a 990-bp fragment from T. atroviride 11 and a 1.5-kb fragment from T. harzianum 2413, using an annealing temperature of 68 degrees C. These fragments showed no significant homology to any sequence deposited in the databases. The primer pair, BR1 and BR2, was designed to the 1.5-kb fragment amplified from T. harzianum 2413, generating a SCAR marker. To test the specificity of these primers, experiments were conducted using the DNA from 27 Trichoderma spp. strains and 22 field soil samples obtained from four different countries. PCR results showed that BR1 and BR2 amplified an 837-bp fragment unique to T. harzianum 2413. Assays in which total DNA was extracted from sterile and nonsterile soil samples, inoculated with spore or mycelium combinations of Trichoderma spp. strains, indicated that the BR1 and BR2 primers could specifically detect T. harzianum 2413 in a pool of mixed DNA. No other soil-microorganisms containing these sequences were amplified using these primers. To test whether the 837-bp SCAR marker of T. harzianum 2413 could be used in real-time PCR experiments, new primers (Q2413f and Q2413r) conjugated with a TaqMan fluorogenic probe were designed. Real-time PCR assays were applied using DNA from sterile and nonsterile soil samples inoculated with a known quantity of spores of Trichoderma spp. strains.Microbial Ecology 02/2005; 49(1):25-33. · 3.12 Impact Factor - Fungal Genetics and Biology 01/2005; 42:924-934. · 3.26 Impact Factor
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ABSTRACT: Trichoderma is known for being the most frequently used biocontrol agent in agriculture. A fundamental part of the Trichoderma antifungal system relies on a series of genes coding for a variety of extracellular lytic enzymes. Characterization of the polymorphism between five putative isoenzymatic activities [beta-1,3-glucanase (EC 188.8.131.52, EC 220.127.116.11), beta-1,6-glucanase (EC 18.104.22.168), cellulase (EC 220.127.116.11; EC 220.127.116.11, EC 126.96.36.199), chitinase (EC 220.127.116.11, EC 184.108.40.206), protease (EC 3.4.11; EC 3.4.13-19; EC 3.4.21-24, EC 3.4.99)] was carried out using 18 strains from three sections of Trichoderma. Of these, seven strains were from T. sect. Pachybasium, nine from T. sect. Trichoderma and two from T. sect. Longibrachiatum. Thirty-seven different alleles in total were identified: 13 for beta-1,3-glucanase, four for beta-1,6-glucanase, three for cellulase, eight for chitinase and nine for protease activity. A dendrogram (constructed by the unweighted pair group method with arithmetic averages) based on isoenzymatic data separated the 18 strains into three main enzymatic groups: T. harzianum, T. atroviride/T. viride/T. koningii and T. asperellum/T. hamatum/T. longibrachiatum. Isoenzymatic groupings obtained from biocontrol strains are discussed in relation to their phylogenetic location, based on their sequence of internal transcribed spacer 1 in ribosomal DNA and their antifungal activities.Current Genetics 12/2004; 46(5):277-86. · 1.71 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Genetic variability within 69 biocontrol isolates of Trichoderma, obtained from different geographical locations and culture collections and selected as biocontrol agents, was studied. Sequence data, obtained from the ITS1 region of rDNA and a fragment of the translation elongation factor 1 (tef1) gene, were used in a phylogenetic analysis. Phylograms showing similar topologies were generated using alignments containing the ITS1 region or a portion of the tef1 gene. 21 distinct ITS1 sequence types and 17 distinct tef1 sequence types were identified among the 69 isolates. More than 50% of the potential biocontrol strains were grouped within Trichoderma sect. Pachybasium; of these, 81% were grouped within the cluster that included the ex-type strains of T. harzianum and T. inhamatum, and 16% were grouped with T. virens. Within T. sect. Trichoderma, which included 36% of the 69 strains, 56% were grouped with T. asperellum, and 24% with T. viride, T. atroviride or T. koningii. Only 10% of the strains studied were located in T. sect. Longibrachiatum.Mycological Research 09/2004; 108(Pt 8):897-906. · 2.81 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The thiourea derivatives of N-butylmethylamine (3-benzoyl-1-butyl-1-methyl-thiourea) (1), N-ethylisopropylamine (3-benzoyl-1-ethyl-1-isopropyl-thiourea) (2) and the corresponding complexes of 1 and 2 with Ni(II), Co(III) and Pt(II) have been synthesized. The compounds obtained were characterized by elemental analysis, spectroscopic methods (FT-IR, UV-Vis and NMR) and mass spectrometry. Compound 1, crystallized in the triclinic space group. The antifungal activities of compounds 1 and 2 and their corresponding complexes against the fungus Penicillium digitatum and against the yeast Saccharomyces cerevisiae were investigated. In general, fungal growth inhibition was higher with compound 1 and its complexes than with compound 2, except for the Co(III) complex of 2.Journal of Inorganic Biochemistry 09/2004; 98(8):1307-14. · 3.27 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Mycoparasitic Trichoderma strains secrete a complex set of hydrolytic enzymes under conditions related to antagonism. Several proteins with proteolytic activity were detected in culture filtrates from T. harzianum CECT 2413 grown in fungal cell walls or chitin and the protein responsible for the main activity (PRA1) was purified to homogeneity. The enzyme was monomeric, its estimated molecular mass was 28 kDa (SDS-PAGE), and its isoelectric point 4.7-4.9. The substrate specificity and inhibition profile of the enzyme correspond to a serine-protease with trypsin activity. Synthetic oligonucleotide primers based on N-terminal and internal sequences of the protein were designed to clone a full cDNA corresponding to PRA1. The protein sequence showed <43% identity to mammal trypsins and 47-57% to other fungal trypsin-like proteins described thus far. Northern analysis indicated that PRA1 is induced by conditions simulating antagonism, is subject to nitrogen and carbon derepression, and is affected by pH in the culture media. The number of hatched eggs of the root-knot nematode Meloidogyne incognita was significantly reduced after incubation with pure PRA1 preparations. This nematicidal effect was improved using fungal culture filtrates, suggesting that PRA1 has additive or synergistic effects with other proteins produced during the antagonistic activity of T. harzianum CECT 2413. A role for PRA1 in the protection of plants against pests and pathogens provided by T. harzianum CECT 2413 is proposed.Applied Microbiology and Biotechnology 07/2004; 65(1):46-55. · 3.81 Impact Factor -
Article: Genomics of Trichoderma
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ABSTRACT: Trichoderma strains are considered to be among the most useful fungi in industrial enzyme production, agriculture and bioremediation. Much less developed is the idea of looking at these micromycetes as model microorganisms to study and improve the understanding of important microbial interactions, for instance with plants and pests. In fact, the use of genomic approaches to study the complex and fascinating mechanisms that permit Trichoderma to produce large amount of heterologous proteins (this aspect will be reviewed in detail in another chapter of this book), control pathogens and effect plant metabolism and physiology is still in its infancy. Very little has been accomplished to date, even though the need and interest in sustaining both structural and functional genomic projects is widely recognized and has led to funding and start up of several new initiatives. This chapter presents the rationale used for investing in a wide genome effort on these fungi, based on diversity and potential utility of their biological characteristics, and discusses the problems related to the taxonomical discrimination of the various species and strains within this genus. It also describes the methods that have been used to date to obtain useful genomic data especially on T. reesei and a few other Trichoderma species. Finally, we describe the strategy and the preliminary results of a functional genomic project recently undertaken by an International Consortium comprised of academic institutions and small enterprises, which was purposely formed to follow this initiative. This program aims at exploring genetic biodiversity within the Trichoderma genus, providing basic knowledge on the biology of these complex microorganisms, and developing commercial applications in agriculture, food industry and medicine.Applied Mycology and Biotechnology 01/2004;
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Universidad de Salamanca
- • Department of Microbiology and Genetics
- • Centro Hispanoluso de Investigaciones Agrarias
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Helmantica, Castille and León, Spain
Universidad de Cádiz
- Department of Organic Chemistry
Puerto Real, Andalusia, Spain
Universidad de Sevilla
Hispalis, Andalusia, Spain


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'True Detective' Creator Reveals Season 2 Details
Creator Nic Pizzolatto hinted that season two will feature "three leads" and be set in California.
New details for True Detective's second season have surfaced.
Creator Nic Pizzolatto revealed in a recent interview with radio program To the Best of Our Knowledge that he's "working with three leads" at the moment. True Detective's critically lauded freshman run was a two-hander with Matthew McConaughey and Woody Harrelson, who are not expected to return.
"The characters are all new, but I'm deeply in love with each of them. We've got the entire series broken out with a couple scripts, and we'll probably start casting in earnest within the coming month," Pizzolatto said.
STORY: Television Critics Awards Nominations: HBO's 'True Detective' Leads
While the first season was set in the Louisiana bayou, Pizzolatto is moving the show west. "It takes place in California -- not Los Angeles, but one of the much lesser known venues of California. And we're going to try to capture a certain psychosphere ambiance of the place much like we did in season one," he said.
True Detective takes on the anthology format, introducing a self-contained case at the top of each season with a new cast.
Pizzolatto, who took part in The Hollywood Reporter's Emmy drama showrunners roundtable, was aware that McConaughey and Harrelson would likely be a one-season deal. "With two guys like that, I guess the concession would be, we're not going to use them again," he said. "People who do have active cinematic careers aren't going to want to come back for six years or five years."
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Trends: Jefferson, Knicks D, NBA pace
Basketball Prospectus looks at three trends from around the Association
Updated: November 8, 2010, 3:24 PM ET
Kevin Pelton | Basketball Prospectus
Noah Graham/Getty ImagesRichard Jefferson is finally playing at the level the Spurs expected of him.
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The new centre will bring together innovators to develop new technology solutions.
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Big Blue creates a Shoreditch away from Shoreditch.
Michael Allen, VP of APM at Dynatrace, highlights the problems and solutions of current cloud SLAs.
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ICAC told document changes hid cost of AWH deal
PUBLISHED: 04 Apr 2014 18:43:01 | UPDATED: 06 Apr 2014 12:41:26PUBLISHED: 05 Apr 2014 PRINT EDITION: 05 Apr 2014Alterations to a 2010 NSW cabinet minute hid the fact that a $1.2 billion infrastructure deal promoted by Australian Water Holdings would cost the state hundreds of millions of dollars.
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Nicu Care Plans
Neonatal intensive care unit - nicu, Neonatal intensive care unit and nicu information on premature infants, preterm births, and congenital defects. neonatal icu information for parents and professionals.. Nicu neonatal intensive care unit | children' hospital , The neonatal intensive care unit (nicu) is a state-of-the-art, level iv, 59-bed unit. we care for more than 700 infants with various health problems every year.. Neonatal intensive care unit kosair children' hospital, In the kosair children's hospital in louisville, ky neonatal intensive care unit, newborn care and family comfort are top priorities..
5 neonatal sepsis nursing care plans - nurseslabs
Neonatal sepsis is an infection in the blood that spreads throughout the body and occurs in a neonate. here are 5 neonatal sepsis nursing care plans..
Nursing care plan – neonatal pneumonia | nursing crib
Neonatal pneumonia is a pulmonary infection presenting with a clinical picture of respiratory distress, associated with chest radiological findings.


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Has Apple Created Expectations It Can't Reach?
June 11 (Bloomberg) -- Bloomberg Economics Editor Michael McKee examines investor expectations for Apple and whether the company can live up to the standards it has created in the past. He speaks on Bloomberg Television's "In The Loop."
Most Recent Videos
Gross Says Fed May Raise Rates 25 Basis Points in June
46:52 -
(Bloomberg) -- Bill Gross, a fund manager at Janus Capital Group Inc. and former chief investment officer of Pacific Investment Management Co., talks about the outlook for Federal Reserve policy, the U.S. economy and his objectives at Janus Capital.
Gross speaks with Trish Regan on Bloomberg Television's "Street Smart." (Source: Bloomberg)


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Lauren Conrad's Teen Choice Awards hair
Love It Or Hate It? Lauren Conrad's Teen Choice Awards Look
by Beauty
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Is this mermaid hair working for L.C., or do her extensions look way too obvious? Either way, seems like she managed to sprout a few inches since yesterday's Do Something Teen Choice Awards Pre-Party. (Now you'll ace those hair extensions quizzes.) It's up to you to decide if this look flatters her — but either way, at least she's managing to keep her bare shoulders warm!
Vote on all my Teen
Choice Awards polls here!
6 years
Her makeup looks the same as usual. Her hair is slightly different, and i'm not just talking about the extensions, I don't think i've seen her with the sides pulled up like that before. It's pretty.
6 years
She wears the same dress style and the same hair always.....I can't wait till no one cares to photograph her.....
6 years
She wears the same dress style and the same hair always.....I can't wait till no one cares to photograph her.....
Link Love
- Stop tugging on your eyelid to put on liner, and read this — SheFinds
- Hair accessories are making a big comeback this Fall — Allure
- Beauty products from Shark Tank we actually want to buy — The Gloss
- Rocking fake lashes could be as easy as putting on eyeglasses — HuffPost Style
- Makeup methods for masking a nasty cold — Byrdie
- The brush you need for artfully applied eyeliner — Beauty High
- This new body wash alleviates flu symptoms — Teen Vogue
- Quick techniques that will totally revamp your look — Daily Makeover


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Capital Markets
Company Overview of First Atlantic Capital, Ltd.
Executive Profile
Roberto BuaronChief Executive Officer and Chairman, First Atlantic Capital, Ltd. |
Age | Total Calculated Compensation | This person is connected to 2 Board Members in 2 different organizations across 4 different industries. See Board Relationships |
68 | -- |
Mr. Roberto Buaron is the Chief Executive Officer and Chairman at First Atlantic Capital, Ltd. He founded First Atlantic Capital, Ltd. in 1989. Mr. Buaron's background combines investment experience with extensive consulting and management expertise. From 1987 to 1989, Mr. Buaron was a Senior Partner and Executive Vice President at Overseas Partners Inc. From 1983 to 1986, Mr. Buaron served as the Vice President at Smith Barney, Inc., and a General Partner of First Century ...
Corporate Headquarters477 Madison Avenue Phone: 212-207-0300New York, New York 10022 United States Fax: 212-207-8842 Board Members MembershipsChief Executive Officer and Chairman Former Chairman and Chairman of CFP Group Former Chairman Director 1990-N/A Former Chairman and Chairman of BPC Holding Corporation 2002-N/A Former Director, Chairman of Nominating Committee and Member of Compensation Committee
EducationMBA INSEAD MBA Harvard Business School Unknown/Other Education Politecnico di Milano
Other Affiliations |
Annual CompensationThere is no Annual Compensation data available.Stocks OptionsThere is no Stock Options data available.Total CompensationThere is no Total Compensation data available. |
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Posts tagged "win this"
Gift Me! Win A Kohl's $100 Gift Card And Glam Pack!
Huge holiday gift list + small budget = no holiday outfit. Trying to save that last hunk of change for your own holiday outfit can be more challenging than finding a gift for Your Sister Who Has Everything. But every lady has the right to look damn good at holiday festivities. The Frisky wants to…
The Frisky
November 16, 2011
Win This! "Glee: The Music, The Christmas Album Volume 2″
Nobody brings joy to the world quite like the cast of "Glee". If you're a Gleek at heart, or if you're just a sucker for the holiday spirit, you'll want to deck the halls this season with "Glee's" new album, Glee: The Music, The Christmas Album Volume 2 due out November 15. It features the…
Ami Angelowicz
November 10, 2011
Win This! "Footloose" Prize Package
Are you one of those people who just can't stay still when a good tune comes on? Well, here's a chance for your dancing habit to pay off, as Celebuzz, Idolator, Buzznet, Socialite Life, and The Frisky are teaming up to give away a major Footloose prize package. One lucky dancing machine will get to…
The Frisky
October 4, 2011
And The Winner Is … The Steamiest Summer Sex Story!
Earlier this week, we asked you tell us your steamiest summer sex stories in the hopes of winning an "Erotic Summer Survival Kit" from Athena's Home Novelties. It was hard to choose a winner considering how hot all your stories were. Check out the winne's erotic tale after the jump.
Amelia McDonell-Parry
August 5, 2011
Win This! "Erotic Summer Survival Kit" From Athena's Home Novelties
Do the dog days of summer have you and your S.O. staying in nights and hovering around the air conditioner? Sticking your head in the fridge for gratification? Feeling too listless and lethargic to get it on? If so, we've got the giveaway for you! Athena's Home Novelties, an adult toy shop committed…
Amelia McDonell-Parry
August 2, 2011
And The Prize Goes To … The Ultimate Summer Beach Bag Essential!
Sigh. Seriously, I think the hardest part of my job is picking the winners for our various giveaways. You guys post such awesome answers, I end up having to seek second and third opinions on who should take home the booty. Last week, I asked you to tell me your ultimate summer beach bag…
Amelia McDonell-Parry
May 27, 2011
Win This! Limited Edition Hayden-Harnett Scarf & Book By Brenda Novak!
When we attended stylist Robert Verdi's last tweet-up -- in which he unveiled his top Mother's Day gifts -- we fell in love with this limited edition scarf by Hayden-Harnett. Designed in collaboration with author Brenda Novak, the scarf is printed with a passage from her new romance/mystery novel Inside. All…
Amelia McDonell-Parry
May 20, 2011
And The Prize Goes To … The Perfect Ladies' Night Out!
A little over a week ago, to celebrate the hilarious lady-centric movie "Bridesmaids," I asked you to share your ideal ladies' night out scenario -- the commenter with the most entertaining and unique answer would win a "Bridesmaids" Survival Kit and a $100 Cash Card. Though all of your ideas sound like a…
Amelia McDonell-Parry
May 17, 2011
Win This! "Bridesmaids Survival Kit" And $100 Cash Card
You do have May 13 marked down in your calendar as the day when "Bridesmaids" hits theaters, don't you? I saw the movie last night at a screening -- one of the perks of my job, gotta say -- and while I can't give too much away (look for our review on Monday!), I…
Amelia McDonell-Parry
May 6, 2011
And The Prize Goes To … The Best Spring Style Resolutions!
Last Friday, I psyched you guys up for spring with the prospect of owning this fun, flirty, and floral dress from French Connection. I asked you to share your spring style resolutions -- the 10 best answers would win this happy little frock. You made it really, really hard to choose. I wish I…
Amelia McDonell-Parry
March 11, 2011
And The Prize Goes To … The Most Ridiculous (Imagined!) Fashion Hybrid Item
Earlier this week, we asked you to tell us the most ridiculous fashion hybrid item you could imagine, along the lines of jeggings, jorts, shooties, and my contribution to the fashion lexicon, bradigan. The most original and hilarious suggestion would win Gap's Soft Trench, part of the brand's spring collection. We've picked a winner…
Amelia McDonell-Parry
March 4, 2011
Win This! The Perfect French Connection Dress For Spring
Just look at this French Connection dress. With its floral pattern and bright color, it's literally giving the middle finger to dreary, overcast cold temperatures. And a few lucky readers will be able to join the frock in its defiance of winter and anticipation of warmer weather because we're giving away 10! WIN THIS! Tell…
Amelia McDonell-Parry
March 4, 2011
Win This! Gap's Soft Trench For Spring
Jeggings. Shooties. Jorts. So many hybrid fashion terms, too little time after shopping to memorize them. It's okay, here's one worth remembering -- FLASHION. That when flash sales occur on hot fashion items allowing shoppers the opportunity to grab them before sell out at a major markdown. Gap is having one a Flashion sale of…
Amelia McDonell-Parry
March 1, 2011
And The Prize Goes To … The Best Holiday Treat
Last week, we asked you to tell us how you treat yourself during the holidays, in the hopes of winning your holiday wish list from Avon. You all gave us some great ideas for maintaining our sanity this time of year. But there could be only one winner.
Annika Harris
December 17, 2010
Win This! Your Avon Holiday Wish List
Avon's holiday campaign "Shop Early, Shop Easy, Shop Avon" pretty much sums up why we're big fans of the cosmetic, skin care, and fashion giant. Not only can you get everything you need for the holidays, like the 3-Piece Enchanted Holiday Nailwear Collection or a Wear Your Way Convertible Dre…
Annika Harris
December 10, 2010
And The Prize Goes To … The Best Roger Sterling Quip
You know we're huge "Mad Men" fans here, so we were truly excited to read your favorite quips from the resident silver fox of Sterling Cooper Draper Pryce, Roger Sterling, Jr. Thank you for taking us on a short trip down "Mad Men" memory lane and for providing your insight into the character.
Annika Harris
November 24, 2010
Win This! A $1,000 Nautica.com Shopping Spree
As the holidays approach, we all start thinking about that favorite moment spent with friends and family, the meal you're most looking forward to eating, the gifts you are most looking forward to giving (and let's be honest, receiving), and the traditions that come along with all of it. This year, we want to be…
Annika Harris
November 24, 2010
Win This! A Copy Of Roger Sterling's Book
We heard bits and pieces of Sterling's Gold: Wit and Wisdom of an Ad Man by Roger Sterling, Jr. on the last season of "Mad Men." We didn't think we'd be able to read Roger's book, especially since he couldn't get any publishers to bite. But he's quite the character and is know…
Annika Harris
November 19, 2010
And The Prize Goes To … The Most Expensive Impulse Buy
Last week, we asked you to tell us the story of your most expensive impulse buy, in the hopes of winning a $100 gift card from HauteLook. Damn! I thought I was an impulse shopper, but never have I considered buying a house on impulse, like our winner did. So prgirl…
Annika Harris
November 12, 2010
Win This! A $100 HauteLook Gift Card
You know HauteLook for offering daily discounts of 50 to 75 percent off the top brands. But did you know they just launched a new iPhone app, which makes your shopping sprees easier? Through the app, shoppers have streamlined access to the HauteLook's 48-hour sale events; can search for products by size, price, and category…
Annika Harris
November 5, 2010
Simply Irresistible
All »
Jared Leto Cut His Hair And Broke Our Hearts
RIP Jared Leto's Ombre Jesus. Never forget.
Claire Hannum
March 2, 2015
Ryan Gosling Scrawls Daughter Esme's Name On His…
Oh look, it's hottest DILF alive, Ryan Gosling, making a rare appearance in public grabbing lunch in LA today. And what's that I see? In addition to his perfectly worn T-shirt (that collar is stretched just so), hoodie and leather jacket, the Gos is rocking a few faint letters on his knuckles, spelling out the…
Amelia McDonell-Parry
March 2, 2015
Shia LaBeouf Has A Rat Tail, Everyone Go Home Now
Presented without comment, because really, what is there to say? [Photos: Fame/Flynet]…
Amelia McDonell-Parry
March 2, 2015
Carly Rae Jepsen's New Single Is Decidedly…
Carly Rae Jepsen's new song, "I Really Like You," has a chorus that proclaims -- in case the title hadn't made it clear enough -- "I really really really really really really like you." Profound. Nothing wrong with getting straight to the point! The only problem is that it is painfully reminiscent of the "Song That Get…
Claire Hannum
March 2, 2015


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EuropeTurkish police chiefs sacked amid graft raids Turkish Deputy PM says politics will not getting in the way of justice after several high-profile detentions. 18 Dec 2013 19:28 GMT | Europe Turkish media reports put the latest number of detained suspects at 52 [AFP]Turkey's Prime Minister Recep Tayyip Erdogan has said the detention of dozens of people, including police officers and ministers' sons, in a high-profile corruption probe is an "ugly operation" against the government. Five Turkish police commissioners have been sacked a day after the sons of three cabinet ministers and prominent businessmen were detained in a corruption probe, Turkish media reports said on Wednesday. The heads of five departments in the Istanbul police force, including its financial crimes, organised crime and smuggling units, were removed from their posts, various national media outlets reported. Political tensions are running high in Turkey ahead of a a series of elections starting next year that will pose a key test for Erdogan after anti-government protests in June. Erdogan branded the graft probe an "ugly operation" against the government. "We will not allow political plotting," he told reporters in Ankara. Deputy Prime Minister Bulent Arinc said five police chiefs had been dismissed after the raids in Istanbul and Ankara that led to the detention of 51 people. "The judicial process should be concluded swiftly and carefully," Arinc told reporters after a closed-door meeting with Erdogan and several ministers. "We will always respect any decision made by the judiciary and will not engage in any effort to block this process," he added. Scores of people including the sons of three cabinet ministers, senior bureaucrats and well-known businessmen were detained in operations undertaken in Istanbul, Turkey's largest city, and the capital Ankara on Tuesday over a corruption investigation. Those detained include the sons of Interior Minister Muammer Guler, Economy Minister Zafer Caglayan and Environment Minister Erdogan Bayraktar. Various well-known businessmen and bureaucrats were also detained including Suleyman Aslan, the chief executive of Turkish state bank Halkbank, Mustafa Demirand, the mayor of Istanbul district Faith, and Ali Agaoglu, a renowned construction tycoon. The suspects are accused of accepting and facilitating bribes to secure construction permits for protected areas, the Turkish media reported. Some reports on Wednesday also claimed that $4.5m have been seized at Aslan's house, this has not been confirmed by officials yet. 'Power struggle' Many analysts linked the sweeping sentences to powerful United States-based cleric Fethullah Gulen, whose followers have long held influential positions in state institutions from the police and secret services to the judiciary and Erdogan's ruling Justice and Development Party (AKP). The tensions between the two fronts arose after the government's plans to abolish private prep schools that prepare students for the Turkish central university exam. Gulen owns a large network of such schools. Gulen supported Erdogan's AKP since 2002, but the bitter row between the two in recent weeks risks fracturing their support base before local and presidential elections next year. The cleric, 72, has been living in exile in the US since 1999 to escape charges of plotting against the secular state but his movement still wields considerable influence within the status apparatus in Turkey including the police and the judiciary.Source: Agencies
Turkey's conservatives coalesce under Erdogan The AK Party has united social conservatives and presided over a burgeoning economy, but also left the nation divided. Politics, Iraq, Syria, Turkey
Erdogan retaining support despite protests Despite weeks of demonstrations, and accusations of brutal force by police, many still back Turkey PM. Politics, Europe, Turkey
The other side of Somalia's pirates Poisoned waters and illegal fishing drove Somalis into sea piracy to feed families, according to relatives. Human Rights, Poverty & Development, Environment
The most powerful mafia you've never heard of Italy's 'Ndrangheta crime syndicate is believed haul in more revenue than many Fortune 500 companies. Italy, Crime, Europe
New Afghan militia sets its sights on ISIL 'Marg' vigilante group vows to safeguard northern Afghanistan from armed factions amid reports of ISIL infiltration. War & Conflict, Asia, Afghanistan
Devastation and disease after deadly Malawi floods Tens of thousands stranded after southern Africa country's record floods face rising risk of disease outbreak. Humanitarian crises, Africa, Malawi
Turkey's conservatives coalesce under Erdogan The AK Party has united social conservatives and presided over a burgeoning economy, but also left the nation divided. Politics, Iraq, Syria, Turkey
Erdogan retaining support despite protests Despite weeks of demonstrations, and accusations of brutal force by police, many still back Turkey PM. Politics, Europe, Turkey
Egypt's 'terrorist' labelling of Hamas prompts protests Palestinians across Gaza denounce ruling and reject Egypt's accusations that the group is aiding armed forces in Sinai. Politics, Middle East, Egypt, Mohamed Morsi, Hamas
Opposition to hold vigil for murdered Kremlin critic Detectives search for clues after killing in Moscow of Boris Nemtsov that has increased concern about Russia's future. Politics, Russia, Vladimir Putin, Europe
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People & PowerKilling the BallPeople & Power investigates how a match-fixer and his syndicate corrupted global football.Sport, Football, Corruption


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Featured news
The latest bogus AmEx email to hit inboxes is a warning about an aborted transaction / terminated incoming transfer:
Unfortunately, all of the links included in the email will take the worried user to one of many compromised websites hosting the Blackhole exploit kit.
The kit then exploits an Adobe Reader and Acrobat vulnerability to drop an information-stealing Trojan onto the victim's machine.
According to Webroot, when this malicious campaign was first spotted and analyzed more than a week ago, the malware in question was detected only by two of the 43 AV solutions used by VirusTotal. Since then that number has reached 15, but that is still a very poor detection rate.
If you have been duped into clicking on the links you should consider seeing which AV solutions detect the malware and use one of them to check your machine.
(IN)SECURE Magazine issue 45 released
(IN)SECURE Magazine is a free digital security publication discussing some of the hottest information security topics. Learn about personal data bankruptcy and the cost of privacy, security and compliance, delivering digital security to a mobile world, and much more.
Windows vulnerable to FREAK attacks after all
Microsoft has released a security advisory on Thursday, confirming that all supported releases of Microsoft Windows are vulnerable to the recently documented FREAK attack.
Banking Trojans target nearly 1,500 financial institutions
Nine of the most common and sophisticated financial Trojans in use are targeting 1,467 financial institutions in 86 countries, says a Symantec report.
HITB Haxpo: Connecting hackers, makers, builders and breakers
Youri van der Zwart is running HITB Haxpo - a free exhibition for hackers, makers and software developers taking place alongside the Hack In The Box conference in Amsterdam, 27th - 29th May 2015.
Black hole routing: Not a silver bullet for DDoS protection
As ISPs, hosting providers and online enterprises around the world continue suffering the effects of DDoS attacks, often the discussions that follow are, What is the best way to defend our networks and our customers against an attack?
Reading our newsletter every Monday will keep you up-to-date with security news.
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What's common between cinestars Amitabh Bachchan, Dharmendra, Shashi Kapoor, Zeenat Aman, Neetu Singh and Hema Malini? They proudly flaunted oversized, colourful bell bottoms on the silver screen during their prime. But the style fails to impress Manish Malhotra, one of Bollywood's favourite designers.
Life & Style | Beauty & Fashion
Bell bottoms not impressive, says Manish Malhotra
The style fails to impress Manish Malhotra, one of Bollywood's favourite designers
- Image Credit: AFP
- Indian Bollywood actor Shah Rukh Khan (L), Indian fashion designer Manish Malhotra (C) and Indian Bollywood actress Deepika Padukone (R) pose during the Grand Finale of PCJ Delhi Couture Week 2013 in New Delhi on August 4, 2013. AFP PHOTO/ SAJJAD HUSSAIN
Bell bottoms became a rage around the 1970s and 1980s, but Malhotra tags them as a "mistake".
"I was uncomfortable with the 1980s era in Bollywood. I always thought the trend of oversized bell bottoms was a huge mistake for anybody," Malhotra said.
A string of popular actors and actresses of those times often flaunted bell bottoms with tucked in shirts or loose peasant tops, teamed with oversized sunglasses to reflect a retro look. Remember movies like "Hare Rama Hare Krishna", "Trishul", "Maha Chor" and "Yaadon Ki Baraat"? They created a fashion trend of sorts - with several young boys and girls getting motivated to throng markets to buy their pair.
Malhotra feels that "styling, fashion and clothes have always been the essence of any Bollywood film", and he is especially smitten by the way leading ladies of 1950s and 1960s dressed up on screen.
"I've always been inspired by the old world charm and grace of the leading ladies in the 1950s and 1960s. In movies like 'Amrapali', 'Pakeezah' and 'Umrao Jaan', the styling was over-the-top and very catchy. The elegance of the chiffon saris in the 1960s has also been something that has inspired me greatly," said the designer.
He is here to present the grand finale show at the ongoing Delhi Couture Week.
His own romance with Bollywood's ever changing fashion sensibilities began when he worked as a costume designer for actress Juhi Chawla in the 1989-released film "Swarg". Later, his makeover for actress Urmila Matondkar for the 1995 film "Rangeela" won him his first Filmfare award.
The going became smooth for Malhotra as he went on to style screen goddesses like Kajol, Karisma Kapoor, Kareena Kapoor, Rani Mukerji, Aishwarya Rai Bachchan... Preity Zinta... And the list continues with the current crop of leading ladies Deepika Padukone, Sonam Kapoor and Alia Bhatt.
Fashion trends in cinema keep coming back, he said, explaining: "Every era comes with a classic trend. Fashion is a recurring phenomenon. Like the anarkali trend, black and white colours and structured Bandhgala jacket trend have all surfaced once again to complete a full circle," said the 48-year-old, who himself exudes style.
In 1998, the designer decided to venture into mainstream designing with his couture store 'Reverie - Manish Malhotra'. He staged his debut fashion show a year later. He made inroads in the international fashion circle when he designed for late pop legend Michael Jackson when he visited India for a Bollywood show.
Malhotra also styled actress Reese Witherspoon in Mira Nair's "Vanity Fair", actor Jean-Claude Van Damme and pop star Kylie Minogue. He takes pride in the fact that the Indian fashion industry has covered miles on foreign shores.
"The fashion industry in India has evolved beautifully, particularly in the last 20 years, when the boundaries of the industry started to expand. The West is learning that India is glamorous and that the sari is the most sensuous garment in the whole wide world," he said.
He also believes that the mind-blocks surrounding 'accepted' fashion no longer exist.
"Men have now embraced pink overcoming the stereotypical thinking. An anarkali is as stylish as an evening gown - we see a lot of celebrities embracing our Indian culture like the Bandhgala Jacket and the sari at various international events. Many such examples tell us the story of an evolving fashion industry," he added.
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smashed iphone5 for swap
white iphone5 16 gb on ee smashed screen when i dropped it so all thats needing is a new screen looking to swap for an xbox 360 package or a tablet email me to let me know thanks
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white iphone5 16 gb on ee smashed screen when i dropped it so all thats needing is a new screen looking to swap for an xbox 360 package or a tablet email me to let me know thanks
IPHONE 5 smashed screen been dropped was working till screen smashed so new screen £30 max and will have a working iPhone the phone is on ee its 16gig white looking to swap or an xbox 360 hopefully with games can only email collection is from my hom
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phone looks new , unlocked , no iCloud , box , charger , sell or exchange with another phone
Hi I'm selling my iPhone 6 plus 128gb finished in rose gold. The phone comes boxed with cable. It's in mint condition and on ee network( Orange t mobile Virgin). Will ONLY swap for iPhone 6 with CASH MY WAY. Thanks.
I-phone 4s for sale perfect working condition looks brand new £80 may swap for samsung galaxy
Looking to swap my iPhone 5s for something round the same price range must be a phone if you offer me something that's cheaper I will not contact you back thanks. If I am selling it's 250 thanks
Swap my 1 month old gold iphone 5s 16gb locked to vodafone, great condotion comes with box etc. Looking for samsung s5, note 4 or anything decent no silly offers and time wasters, swap only
Hi, I'm selling my iPhone 5 32gb Factory Unlocked black in colour. It comes with box and accessories. It's in excellent condition, still has protector on back and always been in a cover. I'm looking for £200 or swap with Samsung Galaxy S5 plus ca
Looking to swap my iphone 5c in white 8gb on ee tmob orange gor a samsung galaxy s5 this phone has a spotless screen is imei clear not on contract and check mend clear. swap in confidence thanks for looking
Hi Today for sale/trade I have an iPhone 6 plus 16gb. It is slate gray colour, unlocked to all networks. I have the proof of purchase as well. It is boxed with all accessories which are brand new (I was using my iPad charger and bluetooth headphon
Hi I have for sale my apple iphone 5c in white 8gb in great condition. Screen is spotless. a great phone not on contract no icloud and imei clear. a cracking phone on the ee network. That usually means ee tmobile orange .comes with charger plug
Hi for sale is my Apple iPhone 6 16gb space grey looking to either sell for 450 Ono or swap for a Samsung Galaxy s4/s5 and cash my way. Reason for sale/swap is I prefer Samsung If swap phone must be on Vodafone or unlocked also good condition
Wanting to swap for another iPhone please!! Really dislike how the backs white , just not a big fan of the 5c shape / colour! Phone works perfectly - must be on 3 or unlocked. This phone is on 3
Apple iphone white 8gb in great condition only selling or swapping as I want to go to android great phone in good condition imei clear mo contract currently on ee tmob ect but can easily be umlocked for £20 Grab a bargain as this a high quality
Hi looking to swap my iphone 5 c for a galaxy s5 Just want to go back to android this phonevis contract free imei clear abd in good condition if interested give me a call text or email stevie thanks
Hi im looking to swap my iphone 5c in white for a sansung galaxy s5. I got the apple but would prefer togo back to samsung imei clear pay as you go no icloud or find my phone. can meet local Please text phone is in white and screen has no scrat
Wanting to swap for a 5s or 5 but preferably a 5s and preferably black. I also have a iPhone 4 unsure if it's a 4s or not. But would sell that along with beats for a good iPhone 😊 text if interested too swap! Nothing wrong with the phone I just disl
iPhone 6+ 16GB grey brand new condition O2 network boxed for sell 420 or swap for Note 4 + £80 Blackberry passport + £100 Thanks
Brand new Apple iphone 6 gold 16gb fully boxed with accessories on EE t-mob Orange network will sell for £380 or swap for a iPhone 6 gold unlocked with cash call 07460992225
I have 2 iPhones 5 one is black 64 gb on O2 one white 32 gb on ee and one iPhone 5s White 16 gb on Vodafone swap with iPhone 6 unlocked texts only


text:
(171) Comments | Posted July 31, 2009 | 5:01 PM
At the beginning of the Obama-McCain campaign, I sent an e-mail to Tavis Smiley. He was to host the presidential debate between the candidates. I asked Mr. Smiley if he would ask the candidates a question from me as a retired police detective and expert on the subject of police...
(16) Comments | Posted June 30, 2009 | 10:15 AM
I am sure the police officer who shot Omar Edwards on the streets of Harlem did not intend to kill an innocent man, black or white - let alone a fellow officer.
He shot his victim out of fear.
Fear has been the accepted justification for such shootings. The officers...
(158) Comments | Posted June 22, 2009 | 1:00 AM
"What did you think of the shooting in the city the other day?" my friend on the other end of the line asked. He's a retired NYPD lieutenant with 20 years of service.
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Kid Wanted To Get Punched and He Got What He Asked For Sometimes you should be careful of what you ask for. 1/8/2015 11:58:35 AM 20,287 19
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Body recovered near Natuashish in Labrador is missing teen: Innu chief
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Re: Times editorial Union's now the bully, Jan.10
Kudos! You're right on. Parents and students are currently subjected to a great deal of unnecessary stress and uncertainty by teacher walk-outs and union threats to withdraw their voluntary extra-curricular involvement until 2014.
Enough is enough; teachers, please stop further walk-outs on your students. As teachers care deeply about their students' well -being, walk-outs (and similar planned school disruptions) do not provide supports to students in their learning. Walk-outs achieve little more than frustration and havoc to parents' daily work/routine.
Much has been said about the "injustice" caused by Bill 115 (imposed contract) to teacher bargaining rights, but their perceived injustice is, in essence, self-inflicted. And honestly, it's actually the result of its union leaders abject failure and inability/unwillingness to negotiate an agreement with school boards during the entire past year, prior to Dec. 31, 2012.
Yes, the government has been heavy-handed and Bill 115 is a drastic measure in dealing with the provincial drastic deficit. Yes, there was Liberal mismanagement of our economy. Yes, there were also generous labour and teachers contracts wage increases done (with other benefits) in the past four years, all of which contributes to the current deficit level.
The "injustice" done to teachers unions is that simple.
Now a recent ruling decrees walk-outs and strikes are illegal. Yet, union leaders vowed to keep up the protest and direct teachers to implement similar walk-off/disruptions inside the schools, as "business is not as usual".
Parents and students are not stupid; they are aware that the majority of teachers are uncomfortable with walk-outs and union bullying tactics. As educators, teachers wants to teach and to be there with students and to enhance their learning, not just walk off their job. But they don't want to be ratted out either.
Using students and parents as political pawns in a labour dispute is fundamentally wrong. There's a case to be made that the union is now the bully.
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Star Tracks
The pop diva gets keyed up in New York City. Plus: Mariah, Nick & Vanessa and more
Credit: Humberto Carreno/Startraks; INF
Published: Thursday Aug 31, 2006 | 04:40 PM EDT
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Family-owned, independent, plumbing supply company - est. 1950 -
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Complete Home Remodeling is the best Atlanta contracting company. We are state licensed for all types of remodeling including residential and commercial remodels. If you need it... Read More
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New Ground
for a
Find out how
New Event
Free Weekend Service for SunRail
Sunrail is offering FREE weekend service March 21-22 to the Winter Park Sidewalk Art Festival.
Why choose Winter Park Memorial Hospital?
With a mission to extend the healing ministry of Christ within our walls and well beyond, Florida Hospital Winter Park is using its expertise, programs and partnerships to make our community one of the healthiest in the U.S. Learn how our proactive health and wellness initiatives are making a difference for our employees, patients and the community at large.
The new Florida Hospital for Women at Winter Park Memorial Hospital fulfills all of your needs under one roof with our one-stop boutique approach for your mind, body and spirit called Full Embrace Health Care. Our network of women's physicians provide you with comprehensive women's services and the latest in wellness and health advancements, including your very own Life Designer.
What's Happening
The Prego Expo is back! Presented by Florida Hospital March 29th from 12-4pm. Learn how to prep your body for pregnancy, try out the best baby product...continue reading
#TBT to 1986 Florida Hospital Tampa was the first in the area to offer MRI and we are still innovating after all these years....continue reading
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text:
Roadworks in Leicester from Sunday, February 22
New work starting week commencing Sunday, 22 February:• Welford Road: demolition of New Walk Centre. Exclusion zone in place. Roads in the exclusion zone...
As one of the leading car dealers in Leicestershire, Sturgess Motor Group is proud to provide drivers across both Leicestershire and the rest of the UK. Established over 110 years ago, we have a legacy of exceptional customer service, which continues today throughout our business.
445 Narborough Road
Telephone: 0843 775 6223
5,114 miles, Automatic, Saloon, British Racing Green, 2014, 64 plate, Petrol
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1 commentEvery once in a while Ferrari rolls out a limited-run special....


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Kuwait is investigating a plan to extract shale gas from its northern fields, a Kuwaiti oil sector official said on Wednesday, estimating that the Gulf Arab state could produce 150-200 million cubic feet per day under the right conditions.
OPEC member Kuwait is a major oil producer, with a capacity of 3 million barrels of oil per day. However its gas production is relatively low and it depends in part on gas imports to serve its energy needs.
The official, who spoke to Reuters on the condition of anonymity, said that if Kuwait goes ahead with the plan it would work with a company specialised in shale gas production, without giving further details.
Kuwait's state-owned oil company Kuwait Petroleum Corp was not immediately available for comment.
Production will depend on the technology available "because our main problem is that the (gas) reservoir is complex...and this layer is the most complex," the official said, referring to the part containing shale gas.
The official declined to give a time frame for when production could start, saying that there have only been discussions so far.
Neighbour Saudi Arabia, the world's biggest oil exporter, has begun investigating its large unconventional deposits and their potential for fuelling long-term growth for its booming population.
It has been inspired by a shale gas surge in the United States, which has transformed the country from the world's largest gas importer into a budding exporter.
Disclaimer:The view expressed here by our readers are not necessarily shared by Arabian Business, its employees, sponsors or its advertisers.
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TD-Complete Submission
What to expect when you're expecting (an acceptance letter)
4.15 / 5.00 4,524 Viewshope you all enjoy!
roses are red facebook is blue no mutual friends who the fuck are you?
well that was amusing
all that happened just from taking the phone and the computer away wow thats mest up


text:
Choose Between Two Options
- $45 for one 45-minute From the Sea mud wrap ($90 value)
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Prema Day Spa
The team of aestheticians, nail techs, and massage therapists at Prema Day Spa create a relaxing environment for everything from quick facial massages to soothing all-afternoon visits. Highly trained nail technicians beautify nails with polish and aestheticians revitalize skin with facials 30 minutes or longer. Massage therapists mollify muscular troubles using flowing Swedish strokes or warmed stones that completed their postgraduate work inside a volcano.
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We wanted to let you know about two new features coming soon.
First, we are making Kindle newspapers and magazines readable on our free Kindle apps, so you can always read Kindle periodicals even if you don't have your Kindle with you or don't yet own a Kindle. In the coming weeks, many newspapers and magazines will be available on our Kindle apps for iPad, iPhone and iPod touch, and then we'll be adding this functionality to Kindle for Android and our other apps down the road. Our vision is Buy Once, Read Everywhere, and we're excited to make this possible for Kindle periodicals in the same way that it works now for Kindle books. More details when we launch this in the coming weeks.
Second, later this year, we will be introducing lending for Kindle, a new feature that lets you loan your Kindle books to other Kindle device or Kindle app users. Each book can be lent once for a loan period of 14-days and the lender cannot read the book during the loan period. Additionally, not all e-books will be lendable - this is solely up to the publisher or rights holder, who determines which titles are enabled for lending.
We will post to the forum later this year when these features are available.
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Eckie Prater
I am a thief.
I take a sliver of light before it is gone forever.
Images here are that fleeting glimmer,
an instant of energy that will never be seen again.
I take from the endless immortal spectrum
without really taking.
Here they are,
presented to you.
One thief to another.
Find me at: http://picasaweb.google.com
Adios Pantalones After Dark on the Playground - From the Series, Night School.
25 Jun 2008 — 53 views
Detail: Carving on Shell 900AD - Spiro Mounds Native Culture Education Center.
23 Jun 2008 — 124 views
Detail: Grain grinding exhibit - Spiro Mounds Native Culture Education Center.
23 Jun 2008 — 90 views
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Q: has anyone bought the leash and cuff quick connect straps wondering how they work in real life
A: Do you know?


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If I upgrade Live Lite will all my existing Live Sets in Lite be safe
I am about to upgrade Live Lite to the full version. Will all my Live Sets in Lite be safe and transfer to the upgrade with out getting lost or changing? Or should I store them some where extra to be safe?
I dont want to loose all my hard work.
1 answer
240 answers
257 votes received1 voteyes. you will be able to open all of your sets with the standard or suite version.
1 year ago | 0 comments
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Best Schools in Presque Isle
Presque Isle High School Public • Grades 9-12
570 students • 15 student/teacher -
Eva Hoyt Zippel School Public • Grades 3-5
329 students • 14 student/teacher -
Presque Isle Middle School Public • Grades 6-8
422 students • 13 student/teacher -
Presque Isle Reg Career & Tech Center Public • Grades 9-12
Unknown student/teacher -
Pine Street Elementary School Public • Grades K-2
297 students • 17 student/teacher -
Opportunity Training Center Private
32 students • 8 student/teacher
Which schools are assigned to my home?
About Presque Isle
Presque Isle has 8 schools. One of the highest rated schools is Presque Isle High School, which has a rating of 9. It has 570 students. The public schools in Presque Isle are in Regional School Unit 79/Msad 01. Easton School Department is close and has lesser ratings than Regional School Unit 79/Msad 01. Nearby Regional School Unit 86/Msad 20 scores lower, with a rating of 4.
School Districts Near Presque Isle
Disclaimer: School attendance zone boundaries are supplied by Maponics and are subject to change. Check with the applicable school district prior to making a decision based on these boundaries.


text:
Uniformity of hardscape materials
I'm currently laboring to turn my neglected side yard into a nice side yard. But while I do that, my mind tends to wander and now I'm thinking about the design of my front yard. As I was tending to (i.e. mowing) the front yard I got to thinking about the "plan". I plan on getting rid of some of the grass and replacing it with planting beds. As the idea ferments in my brain, I've gotten stuck a little with the idea of bed outlines and whether the edging material should be uniform.
What I have: My front yard is about 15' deep by 35-40' wide. About 2 ft from the sidewalk is a small retaining wall (about 12 inches high). The wall is made of small granite blocks which are angular. Nearer the house are some planting beds and they are edged with river rocks which are round. The disparity of the materials isn't a problem because they're not serving the same purpose and aren't that close to each other.
What I want: One of my ideas is to create a bed along the edge of the driveway which would run from the wall towards the house. I was thinking of using more of the granite instead of the river rock to tie it into the wall. This bed wouldn't be contiguous with a bed along the house - there would be a narrow path separating it from a bed extending from the house. I was thinking of using the river rock as the edging material for this bed because it's there and I want to use some of the rock as a splash area for my downspouts which come down in roughly the sides of the house bed so I was just going to extend it into the edging.
Where I'm stuck: Would it look strange? Both materials (granite and river rock) are rock but they're very different. They would now be in relatively close proximity to each other and serving the same purpose (bed edging). I do like to have my beds edged with something so not putting something down isn't really an option.
I'm thinking of just ditching the river rock except for the splash downs and using it somewhere else in the yard but is that necessary? If it was rock and wood or some completely different materials, I wouldn't be as stressed but it seems like very different kinds of rock are another story. Am I making this a bigger problem than needs be? In your opinion, should edging of paths/beds be made of the same material when they're in the same small yard? What exceptions can be made and when?
I think those materials go beautifully together. But it of course depends on the design, I'm really bad with written explanations so it's very hard to give an opinion. Potentially it can look really good.
If the yard looses uniformity then maybe add river stone more prominently in other areas of the yard, for example piles of round stone look really nice when strategically placed. This way both materials look like they belong in the garden and work well together.
*my comments are not based of any kind of official education I'm a complete amateur*
Temes, You should try writing your explanations because it will make you understand why you know what you know instead of just "feeling it". It might take a while, but it is very powerful self learning experience.
The question is very general and I think Temes gave you a good general answer. Materials can be mixed. Sometimes they mix well from the get go, sometimes they need help. Almost anything in a landscape can be made or broken by whatever else is either supporting it, mitigating it, or fighting it.
I agree that they can be mixed and matched somewhat but if you had a walkway made of flagstone that suddenly changed to brick, it would look odd. That's a situation that would need help to look okay. I'm worried I'm setting myself up for a situation that's going to need help from the get go and would like to avoid it.
Took these images this evening - it was raining (again) so the material is darker than it is while dry.
First photo is of the wall of granite blocks:
These are the blocks used as edgers:
Here are the river rocks as edgers:
So, my issue comes when I'm thinking about this area:
An idea I was considering is:
Where the little circles are the river rocks and the repeated square-like things are the granite.
And this is where I think the disparity in materials would need help from the start.
I think the river rock is not working well for you where you currently have it. The river rocks are small and not built into any substantial mass, so they have no meaningful impact. By contrast, your granite block wall has enough substance to it to make a meaningful impact. Therefore, I would edge all the beds with granite blocks to tie in with the wall, and get rid of the river rock (or use it for something other than edging beds, preferably something with enough substance to have an impact).
To some extent I think you're focussing on details at the expense of the big picture. From a distance these two materials are likely to be almost indistinguishable anyway, both being grey and pretty small.
As Laag says, I mix hardscape of all kinds, partly because I get bored easily, partly because I'm commitment-phobic. But also, I just find one material doesn't meet the needs in all places - in one spot I want big slabs, in another small bricks will better achieve my objective.
So to the extent that it's successful design (Ink hates it, but thanks Laag), I think that comes from the fact that function is always served by the material used, although I do try to pay attention to contrast, continuity, harmony, repetition, and other strategies for making it look good.
And so it's function that bothers me most about what you've shown and what you're planning: neither of these materials look like really useful edging to me. Weeds and grass can grow through them all too easily and they look difficult to mow beside. But if that isn't a problem for you, then appearance-wise I think that visually they mix just fine. And they will both have some tie-in to functions as the square stone functions in the wall and the river rock will function in the splashdown area.