text:
East Valley water managers have long been a confident bunch.
Yes, we occupy one of the driest places on the planet. Yes, our highways resemble an inbound river of moving vans. Yes, we all know the mantra: "Water — Use it wisely."
But the people in charge of our water have seldom expressed any public worry about whether unbridled growth might outstrip our supply of the precious stuff. We're not like other places with only one or two water sources, they have said. We are blessed with three, and our water supply is as sturdy as a three-legged stool.
What would happen, though, if someone took a saw to one of the legs?
That possibility has begun to loom in recent months, with federal officials talking ominously about cutting the East Valley's share of Colorado River water as soon as 2006.
Two key factors are involved.
First, the West is suffering the worst drought in the 98-year recorded history of the Colorado River basin. Lake Powell, a huge reservoir on the Arizona-Utah boundary, now holds less than half the water it stored only five years ago. Lake Mead, between Arizona and Nevada, is only about half of its capacity.
Second, Western water law dictates that some Colorado River users are entitled to their full allocations even if others get no water at all. The East Valley would be one of the losers under that setup.
Our share of Colorado River water — one leg of the proverbial stool — comes to us via the Central Arizona Project Canal, a $4 billion, 336-mile-long engineering marvel whose completion in 1993 was hailed as a godsend for our thirsty and booming state. It lifts water from Lake Havasu on the Colorado River, brings millions of gallons a year to the East Valley, then wends its way through Pinal County and on to Tucson.
The other two legs of our water supply are the Salt River Project's system of dams and reservoirs on the Salt and Verde rivers, and a large aquifer that underlies the Valley.
Each of those could be affected if CAP deliveries were cut. The impact would depend on the extent and duration of the cutbacks — things that nobody can predict at present. It also would depend on the outcome of frequent droughtmanagement meetings now being held among federal officials and the seven Colorado River basin states.
Concern over the East Valley's CAP supply bubbled to the surface this spring when Bennett Raley, the federal government's top water official, made a highly publicized tour of the Grand Canyon.
Raley, an assistant secretary of the Interior and chief overseer of federal water policy, said then that the drought may force the government to declare a water shortage on the Colorado. "If current trends continue . . . the secretary (of Interior) would be forced to take action, certainly within three years and potentially within two," Raley said then.
CAP would be the most likely early casualty of such a declaration. When Congress approved the project in 1968, Arizona agreed the CAP would be designated as a "junior" user on the Colorado system. The CAP allocation — 1.5 million acre-feet per year — could theoretically trickle down to zero before, say, California, loses a single drop.
Of that allocation, the five largest East Valley cities — Chandler, Gilbert, Mesa, Scottsdale and Tempe — are entitled altogether to a little over 100,000 acre-feet per year. By itself, that would support only about a third of the East Valley's current population.
The cities have not been using all that, however. Some of the current excess is being stored underground and some is being purchased and distributed by SRP to make up for shortages in its own watershed.
An acre-foot of water is 325,851 gallons. It would cover an acre of ground with a foot of water and is commonly regarded as the amount necessary to support a family of four for a year.
Bob Barrett, a CAP spokesman, said a lot of things could mitigate the final impact, even if CAP does lose some of its water.
For one thing, it's impossible to predict just when a shortage would be declared. "That's like asking me how long a baseball game is going to be in total minutes," he said. While Raley said a shortage could be declared by 2006, Barrett said it's "more likely in 2011."
Further, a turn in the weather could forestall a shortage altogether.
"It just depends on how many storms we get," he said. And by "we," Barrett is not talking about just Arizona. He means the entire Colorado River watershed, a vast area that stretches to Wyoming.
It appears, however, that dryness may be more the norm than the exception in the Colorado basin. When water planners divvied up the river back in 1922, they based their calculations on short-term measurements in a wetterthan-normal period. As a result, more Colorado River water is allocated than actually exists, to the tune of 3 million to 4 million acre-feet per year.
Even if CAP were to lose 500,000 acre-feet, a third of its allocation, Barrett said the short-term effect on East Valley cities and industries would be minimal. Agricultural users, he said, would lose their water first, and many of them could get by with groundwater until the CAP flow is restored.
Further, excess CAP water now stored underground could sustain current delivery levels "for some time," Barrett said.
But a cutback could affect the redundancy that has shored up the East Valley's water supply for decades. Here's one way to illustrate that:
In normal years, SRP gets enough water from the Salt and Verde rivers to meet its obligations across the Valley. But drought has forced SRP to cut its deliveries of Salt and Verde water by one-third for two straight years. It has been buying excess CAP water to make up the difference.
Since 1996, SRP has purchased well over 600,000 acrefeet of CAP water, said Charlie Ester, SRP's manager of water resource operations. Roosevelt Lake, the utility's biggest reservoir, now has only about 572,000 acre-feet in storage.
In theory, Ester said, that means Roosevelt Lake could have been drained by now without those CAP purchases. If CAP allocations are cut, SRP could lose access to that water. It would have to drain the Salt-Verde system even further, or begin large-scale withdrawals of groundwater.
Water managers across the West, however, are struggling to prevent such scenarios. In that regard, Ester said, the drought could be a blessing in disguise.
"Maybe the basin states will come to grips with the fact that there are issues with the way the Colorado River has been allocated," Ester said. "Before you can grow in a prudent manner, you need to know what resources you have. Maybe that's one of the good things that will come of this."
Tom Carr, who oversees Colorado River operations for the Arizona Department of Water Resources, said the seven Colorado River states have begun regular meetings, seeking to delay a shortage and to share the burden if one is declared.
"We have time to plan. We are beginning that planning right now," Carr said.
Dave Mansfield, general manager of water resources for Scottsdale, agreed. The earliest year he's heard for potential CAP reductions is 2008. "We are a ways off in terms of seeing any reductions," he said, and he expects underwater storage of excess CAP water to help see the city through potential shortfalls.
Still, there are long-term worries. The Interior Department recently issued a report predicting increasing water conflicts throughout the West, and in Arizona, as the new century unfolds.
Rob Smith, Southwest field director for the Sierra Club, an environmental group, said the drought is an "early warning" about long-term problems across the West.
"We will come out of the drought cycle but the same problems will remain," he said.
"We are growing extremely fast in population. We don't have enough water for everyone to irrigate cotton and have swimming pools and water features and live the sort of life we live now with low water rates."


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How to Be a Ninja
Want to be a Ninja?!
What is a ninja?
A true ninja is not just someone who wears black, drops down from the ceiling and slices everyone to pieces! Ninjas have been around since at least the 14th century, when guerrilla warfare in feudal Japan called for espionage and assassination--activities which Samurai would not perform because they were forbidden by Bushido, the Samurai Code.
Ninja were called on to be master spies, assassins and specialist warriors, both for and against the enemy. While some aspects of the ninja way may be outdated, the principles and techniques remain valuable for anyone who wants to learn from and emulate the ninja way (ninjutsu). You have to complete all steps to become an awesome ninja.
Download this app for free and learn how to be a Ninja.
♦ Attractive user interface for easy exploring.
♦ Shake your iPhone and get random one.
♦ Select and copy option is enabled for SMS sharing.
♦ From "all" section you can see all ideas and select any idea you like.
♦ You can mark any idea you like as Favorite.
♦ Sharing any idea from this app through e-mail is available. Spread the fun to anyone in the world!
♦ More apps/games are available on "more" section.
Also check out our other apps:
*** We recommend some cool iPhone games: Tap Tap Ants, Tap Tap Love , Tap Tap Bubble, Bubble Tap – most addictive, iWarehouse, Flying Insects.
DISCLAIMER: This application may contain some explicit material, but does not intend to be defamatory, racist or offensive in any way. In no event shall the creator of this application be liable in any manner for any direct, incidental, consequential, indirect or punitive damages arising out of your access, use or inability to use this application, or any errors or omissions in the information on this application.
Email us : firstname.lastname@example.com
Follow us : www.twitter.com/riseuplabs
Subscribe us : www.youtube.com/riseuplabs
Join us : www.riseuplabs.com/facebook
What's new
Improved content and user interface.
Fixed some bugs.
Share with Others
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- Oct 26, 2014
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subset: commoncrawl


text:
Having An A+ Marriage
Apologies are effective when your partner feels that you really know you made a mistake and that you really will do your best not to let something similar happen again. If necessary, it means letting them know you have a plan to take action to minimize the chances of repeating the same behavior. This may include taking a time out when angry, getting up five minutes earlier so you can pick up a little after yourself before leaving for work, or even going to counseling or seeking other outside help if you have a problem you can't seem to manage on your own.
Apologies don't include the word "but," or anything similar. If you want to help your spouse understand what lead to whatever you said or did, that can come later. Otherwise, whether you mean it to or not, it will seem like you are trying to explain or rationalize your words or actions. Say you are sorry, name the behavior you are apologizing for, and humbly state that you know you were wrong and hope the other person will forgive you. It may also be helpful to state that you know your words or actions were hurtful or upsetting so your partner knows you are trying to be in tune with how he/she is feeling.
Attention. Paying attention means being aware and observant in your marriage. It means really "showing up" not just phoning in your participation or physically being present while being mentally somewhere else.
Paying attention or giving attention means your spouse gets pieces of your time and energy throughout the day. This is about noticing what your spouse is wearing, being interested in his/her day, and knowing the things that are important to him/her. Paying attention means remembering to ask about that big work project or stressful situation with colleagues. It means calling just to say "Hi, I was thinking about you," or saying, "You look nice in that sweater."
Paying attention also means looking at your spouse when he/she is talking to you and being aware enough to say, "You look angry/worried/upset. What's up?" It means remembering anniversaries and birthdays and other dates that are important to your spouse. It means setting aside time to focus on your spouse and your relationship. Time should be set aside each week to really talk, go to dinner, or engage in an activity that will allow you to focus on your spouse and nurturing the friendship and romance. This is very important for every couple. But paying attention to your spouse can't just happen during these "designated" times. It also must be done in small ways every day.
Affection. Affection means touch. This does not just mean sex. It does not just mean "making a move" on your spouse or even being flirty, though touch is very important for romance and sexual intimacy.
It means small gestures of physical contact throughout the day. It means brushing your spouse's arm when you walk by, a light touch on the back or shoulder, reaching over to take your spouse's hand while riding in the car, or a playful poke or swat. It means offering a hug when your spouse gets home from a long day at work, rubbing his/her feet, or snuggling on the couch while watching TV.
It is important that touch be given at other times besides in the bedroom or when trying to initiate sex. Touch is a way of connecting with your partner and does not have to have an ulterior motive. It is friendly and supportive and helps your partner feel close to you.


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Hey Guys,
On Sunday, I picked up a Glock 17 Gen 4 and ordered the M&P9, which I picked up Wednesday. I did a quick review on the Gen 4 on Sunday or Monday. I did a review on the Talon II Lightweight Bobtail a while back. This Nighthawk is a very similar gun, but all steel. This is in addition to the Diamond Black custom Nighthawk PDP Commander I just got in. It has black Gatorbacks and an undercut trigger guard that I really like. Great gun, but I'd basically be repeating myself from the last review, the only thing being different is all steel, which I prefer, and the undercut trigger guard.
Now, I had posted about the Gen 4 after 350 rounds. I then put another 150 through it and another 250 through it, so it now has 750 rounds, 100 being Federal HST 124 and 147 grain. Today, before the last 250 rounds, I put in the Ghost Connector and polished all contacting parts. The trigger is now a good bit smoother and a little lighter. It doesn't have as clean of a break now since the Connector and a little more creep, but that's actually ok on this gun.
As for the M&P, I've order the Apex Sear and striker block and may change some springs for a stronger reset. The gun feels great in the hand and has 250 flawless rounds through it. The reset is a little long and weak right now, so I short stroked it a couple times during some drills. I was also shooting a tad low, but I've been running the Glock since Sunday and the only gun I've done any dry fire with this week, so that's ok.
For rapid fire and transitions, I really prefer the Glock at this time, though I know the M&P will climb to the top after I do the trigger work next week. Once getting used to the way the Glock points, I really enjoy it and it's a fun gun.
I don't care for the sights on either right now, but I'll be changing the front sights to TruGlo TFO's and the rears to Warren Tactical single dot tritiums or Heinie Straight 8's with the single tritium dot. So, I'll have them set up exactly as I like eventually, but they're getting there. I also decided to order the Minotaur M-Tac's for the Glock and M&P with a spare body for a Commander 1911 and a spare back for OWB carry plus some mag holders.
As for the feel and aesthetics, I'll give the edge to the M&P. I just like it better, though the Glock is a little more enjoyable to shoot right at the moment. The M&P runs smooth and magazine changes are smooth without having to reposition my hand, just like the Glock. But, since I ordered it with the thumb safety (I'll probably remove it if I can find the fillers for the notches), when I reload or clear a SET-UP malfunction, I noticed I almost grab around the ambi safety.
The M&P came with 2 magazines and I have 2 more coming with the rebate, which should take 3 months or so, if I remember correctly. I'll buy a good number more for both and will replace the basepads with the 10-8 mags. i don't care for the large basepad on the M&P mags and prefer carrying spare Glock mags due to the smaller basepad.
Well, I know I'm rambling here since I didn't plan on writing about the M&P until I ran it with the 17 and the Sigs which I'll do this week, so I apologize. Already set aside 2,000 rounds for the comparisons.
For now, and I imagine it will continue like this, I've been carrying the Glock due to the heat and the salt air when I'm near the beach. I have a feeling the M&P will become a primary gun rather quickly too. I love my 1911's and they'll always have a place in my heart and my belt, I realized that these polymer guns can be a lot of fun and really nice for the range, classes and carry.
I'll post back with a truly thorough review of the M&P, 17 Gen 4, Sig 226 and Sig 229 SCT probably around the end of next week after the allotted rounds are expended, hopefully equally to some extent!
Oh, I apologize for the horrid pictures! The D3's and all of my lenses are at Nikon Professional Services AGAIN due to calibration issues (and sold my backup Canon 5D that I've had for 5 years), so I used a cell phone and it just wouldn't focus. I know this isn't a very good review, so feel free to ask any questions and of you have.
Have a great day!
Here's a pic of my Talon II Lightweight below. You should be able to see the differences where the trigger guard meets the front strap (high cut on steel Talon II) and the hammer and beavertail are also very slightly different. I wish they did this high cut on all their guns. I like the beavertail on the LW just a little better, it's slightly thinner. I'll have that changed eventually if I keep this gun.....or not, who knows!


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Sun, Aug 4, 13 at 18:16
Follow-Up Postings:
It should read Lc. Clayton Waglay var.alba sorry |
- Posted by orchidnick z9Ca (firstname.lastname@example.com) on Sun, Aug 4, 13 at 19:48
Beautiful, love those white cats. Nick |
Thank you |
- Posted by barbara_in_la So. Cal. (My Page) on Mon, Aug 5, 13 at 0:44
Very nice!! |
So elegant! Jane |
I have to agree, vey elegant looking. |
Beautiful bloom, elegant indeed! |
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Gear Review
Wooden Robots
Ride the pine no longer
Get in the starting line-up with this Burton Starter Co-lab. Snap Front closer and jacket to pants interface make this the ultimate jacket to place your bets on. True to starter style with the exception of Burton DryRide waterproofing. Diamond quilted lining with 3M thinsulate will keep you nice and toasty.
View More Details on Burton Burton X Starter Jacket - Men's


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Institute for Economics and Peace
The Institute for Economics and Peace (IEP) is dedicated to promoting a better understanding of the social and economic factors that develop a more peaceful society. It achieves its goals by developing new conceptual frameworks to define peace; providing metrics for measuring peace; and, uncovering the relationship between peace, business and prosperity.
Want to learn more about IEP? Find our interview with IEP's Research Manager Daniel Hyslop here.
Institute for Economics and Peace
PO Box 42, St Leonards
Sydney, NSW 1590
+61 29 901 85 00
Content from this organization
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text:
The View from Abroad
Kenn Jacobine
- Birthday
- June 03
- Bio
- Kenn Jacobine is an international educator currently teaching History and Economics for the American School of Doha, Qatar. He has also taught at international schools in Ecuador, Mali, and Zambia.
His political transformation took place over the course of many years. Starting out naively as a big state liberal, he became a Reagan Republican in 1982. Disillusionment set in with the realization that small government rhetoric rarely translated into limited government actions.
On Christmas day 1992, he became a libertarian. In 1994, Kenn ran for the State Senate in Pennsylvania on the Libertarian Party ticket garnering 5 percent of the vote. He has been active in freedom causes ever since.
- High Taxes Chase More
Companies from Our Shores
July 21, 2014 08:48PM - An Update on How the Minimum
Wage Hurts Workers
June 22, 2014 09:35AM - American Policy on Ukraine
Crisis is Wrongheaded
March 17, 2014 12:07PM - Obamacare and False Claims
March 07, 2014 10:34AM - President is Pushing Another
Failed Policy
February 28, 2014 09:38AM
- "Didn't think so."
July 28, 2014 06:59PM - "This is so typical of
statists. The title of the
article is
"High Taxes
July 26, 2014 04:07PM - "Gary, please define
"fair share".
is, I am about to leave
July 25, 2014 11:13PM - "That's an interesting
piece. I read yours, please
June 23, 2014 07:50PM - "Sky, do you know what a
franchise is? You see a
franchisee is
generally a
June 22, 2014 06:37PM
Kenn Jacobine's Links
The Gold Standard would Prevent Economic Bubbles
The Regime Should be Questioned More than Ever
Why was al-Awlaki Denied His Constitutional Rights as an Ame
On November 25, 2001, American citizen turned Taliban fighter John Walker Lindh was captured on the battlefield in Afghanistan by Afghan Northern alliance forces. Eleven months later after confessing to fighting with the Taliban against the U.S. and its ally in Afghanistan he was sentenced to 2… Read full post »
Is the Solyndra Affair an Impeachable Offense?
Unless you have been under a rock or possibly out of the country (a benefit of the doubt I am willing to extend to my overseas teacher colleagues) you know by now that there is great outrage in Washington, D.C. over the Obama Administration's $535 million loan to green company and… Read full post »
A Return to the Gold Standard is a Must
Money printer extraordinaire and Federal Reserve Chairman Ben Bernanke is at it again. He and several of his central banking buddies in Europe and Asia are going to lend dollars to non-U.S. banks that lack adequate liquidity to operate. Many of the recipient banks are feeling the pinch be/… Read full post »
Bill O'Reilly Should Do His Homework
Last week, O'Reilly Factor producer Jesse Watters confronted Congressman Ron Paul at New England College to ask him why he was not interested in appearing on Bill O'Reilly's show. One thing led to another and eventually Watters asked Dr. Paul about his position on the Gold Sta… Read full post »
Revisionism is Alive and Well
"There is no credibility left for the Republican Party as a force to reduce the size of government. That is the message of the Reagan years."
Ron Paul 1987
Politicians have a tendency to change history to suit their
purposes. The revising of history by Soviet
dictators was le… Read full post »
Will Hurricane Irene be a Blessing in Disguise?
"Ghastly as it may seem to say this, the terror attack (911) -- like the original day of infamy, which brought an end to the Great Depression -- could even do some economic good."
"If people rush out to buy bottled water and canned goods, that will actually boost the… Read full post »
Ron Paul is Right about Iran
During the GOP Presidential Debate in Ames, Iowa, on August 11, the most significant exchange between any two candidates came when Rick Santorum called Congressman Ron Paul out for his position on our relationship with Iran. Santorum, neoconservative extraordinaire, accused the congressman of b… Read full post »
Ron Paul is the Only One
It has become cliché for political pundits to proclaim every four years that the presidential election campaign is the most important in the nation's history. Given that our economy is on the brink of collapse, we are currently engaged in 5 wars, and our civil liberties are under a… Read full post »
Obama Raises More Questions than Answers
It seems like every time I listen to a politician talk I always come away with more questions than answers. Take President Obama's nationally televised speech this week on the debt ceiling crisis. The President spoke to the nation for about 15 minutes but I still don't have a… Read full post »
We Can't Afford to Raise the Debt Ceiling
"The fact that we are here today to debate raising America's debt limit is a sign of leadership failure. It is a sign that the U.S. Government can't pay its own bills. It is a sign that we now depend on ongoing financial assistance from foreign countries to finance our Government&rs… Read full post »
Obama is Wrong about Medical Marijuana as Well
Presidents are supposed to be compassionate. President Obama is showing his true colors as his Department of Justice (DOJ) sent out a memo last week on medical marijuana use. The memo directed the head of the Drug Enforcement Administration and U.S. Attorneys' offices nationwide to… Read full post »
Western Imperialism Strikes Again
What does Bernanke have up his Sleeve after QE2?
One thing is for sure, Federal Reserve chairman Ben Bernanke's second round of quantitative easing (QE2) will come to an end sometime this month. Since November of last year the Fed has pumped close to $600 billion into the economy by buying treasury bonds from the balance sheets of banks… Read full post »
What Obama Should Have Said
President Obama's speech on the Middle East last week was nothing really new. He chastised the usual culprits for suppressing human rights in their countries and assured us all that the United States government would remain vigilant in its pursuit of truth, justice, and the American way w… Read full post »
U.S. Cannot Learn from Past Mistakes
The fact of the matter is that the terrorist attacks of 911 had more to do with the U.S. government's meddling in the affairs of the Middle East for decades than it did as George W. Bush would have us believe because we are free. All one needs to do is… Read full post »
"Where's the Beef"?
Those of us that are old enough and fond of TV commercial history remember the famous Wendy's hamburger chain commercials of the 1980s. You might remember they featured three fickle old ladies that enter a burger joint and are disappointed at the size of the patty on the bun. … Read full post »
Evaluating Obama's Record Two Years in as President
Recently, President Obama kicked off his 2012 reelection campaign. Looking past all the political jabbering of the talking heads and pundits, the most astounding prediction of all about the next race for the White House is that Obama is expected to raise $1 billion for his campaign efforts.&nbs… Read full post »
Bernanke is Still Clueless
It has been a while since I criticized my favorite Federal Reserve chairman, Ben Bernanke. I've been busy teaching school and Bernanke like always has been busy destroying the dollar and with it our economy and standard of living. So, nothing new and out of the ordinary has happened… Read full post »
Austan Goolsbee's Cruel April Fools' Joke
On Friday, the Bureau of Labor Statistics (BLS) released the latest jobs report for the U.S. economy. In March, non-farm payroll employment rose by 216,000 jobs. According to the report, employment gains were seen in both the goods-producing and service-providing sectors. This job c… Read full post »
Dangers are Inherent in the War with Qaddafi
To be sure Muammar Qaddafi is a devil incarnate. For 41 years he has ruled Libya with an iron fist. He has repeatedly participated in terroristic endeavors, even against his own people. As recently as two weeks ago it looked like he was a goner as rebels had taken hold of… Read full post »
Abraham Lincoln: Biggest Fraud in American History
Last Friday, March 11 marked the 150th anniversary of Abraham Lincoln's first presidential inauguration. For generations the virtues of our 16th president and his martyr like demise at the hands of an assassin have been proudly proclaimed to public school students so much so that Lincoln… Read full post »
What's Good for the Goose is Good for the Gander
Last week the Justice Department announced that it would no longer enforce the Defense of Marriage Act (DOMA). Passed by huge bipartisan majorities in both chambers of Congress and signed into law by President Clinton in 1996, DOMA defines a legal marriage as one between a man and a woman. … Read full post »
Some Sensible Gun Laws for a Change
Hooray for the western states! Many of them are in the process of enacting long overdue and sensible gun laws. Perhaps spurred on by the unstable times we live in or just a sense that people should have the ability to defend themselves, states from Wyoming to Arizona are about to… Read full post »
Kenn Jacobine's Favorites
My Lawsuit Against Facebook
Libyan Policy Re-Considered
When The Elite Piss In Your Cheerios
I'm An Okie From Muskogee!
wow, obama vs inequality! ... lip service kabuki theater
Islamic Terror and Reckless Rhetoric on the Far Right
Happy 2015? Don't Let this happen to you!
"Israel – America's Biggest Frenemy"


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Updated: August 16, 2014 12:42AM
A Whiting man has been charged in Lake Superior Court in a crash Sunday in that seriously injured another driver.
Dylan Reeves, 18, of the 1200 block of West Fred Street, was charged with three felonies – operating while intoxicated resulting in serious bodily injury, criminal recklessness and failure to stop after an accident resulting in injury – and eight misdemeanors related to reckless driving, failure of duty and drunken driving.
Police were called at about 7 p.m. to the 11000 block of Wicker Avenue in Cedar Lake, where they found a maroon Buick Regal with extensive front end damage off the roadway on a hill. The car had struck a mailbox post and had driven through landscaping in the area. Police learned that the car had crashed into another vehicle at West 165th Avenue and Wicker Avenue in Lowell and had been followed by several civilian witnesses who caught Reeves and turned him over to arriving officers.
Witnesses told police that Reeves had been traveling north on Wicker Avenue when he crossed the center line between the two northbound lanes and struck a 2004 Ford Escape driven by Wiestaw Szmuc, whose car launched off the road, rolled into a ditch, crashed into a phone box, rolled through a fence and landed on its side. Szmuc was pinned inside the car and was extricated by Lake Dalecarlia firefighters.
Szmuc was airlifted to Christ Hospital in Illinois for treatment.
Witnesses told police after the Regal crashed into the other car, Reeves drove in and out of traffic aggressively and at a high rate of speed.


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Mad for Mexican
(9 Recipes) Created by KCLARK89
Recipes in this Collection
You will think you are south of the border when you try this recipe. It is a meal- one enchilada will fill you up- it did me! Carbs: 22.7g | Fat: 10.5g | Fiber: 4.7g | Protein: 28.3g | Calories: 293.9
Serve up creamy, cheesy chicken enchiladas, and no one will be late to dinner! Submitted by SKEYDOO Carbs: 35g | Fat: 4.5g | Fiber: 2.7g | Protein: 18.3g | Calories: 252
This one-dish meal is great for a weeknight meal or as a appetizer for a party. Serve it with steamed broccoli or a green salad. Submitted by CHEF_MEG Carbs: 30.4g | Fat: 9.9g | Fiber: 8.5g | Protein: 30g | Calories: 326.1
Low fat sloppy joes that don't skimp on flavor! Submitted by RHG2525 Carbs: 21.3g | Fat: 6.6g | Fiber: 5.2g | Protein: 16.3g | Calories: 191.4
This is a great kids' meal, or cut each quesadilla into eight pieces for an appetizer. Submitted by SPCOOKBOOK Carbs: 14.6g | Fat: 9.1g | Fiber: 1.8g | Protein: 25.6g | Calories: 242.5
Tostadas, basically open-faced tacos or tortilla pizzas, are perfect for weeknight meals. Submitted by CHEF_MEG Carbs: 29.9g | Fat: 5g | Fiber: 8g | Protein: 29.8g | Calories: 277.8
This recipe was a finalist in the SparkPeople Un-Chained Recipe Contest! Submitted by KALORIE-KILLAH Carbs: 38.6g | Fat: 11.8g | Fiber: 3.8g | Protein: 28g | Calories: 366.5
These tostadas are healthy, easy to make and kid-friendly. Feel free to top them with extra veggies of your choice! Submitted by CHEF_MEG Carbs: 24.2g | Fat: 8.2g | Fiber: 4.8g | Protein: 24.7g | Calories: 263
This meal is fast and fun, a great way to spice up a dull morning and bring a brunch favorite to the work week. Submitted by SPARKSOLUTION Carbs: 31.4g | Fat: 5.9g | Fiber: 5.5g | Protein: 11g | Calories: 220.3


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Choose a section News Target News Now Walmart News Now
Michaels has launched Cake Boss cakeware, designed by celebrity baker Bartolo "Buddy" Valastro, star of Cake Boss on TLC, and will be leveraging social media to promote the line.
Available in all U.S. Michaels stores, the cakeware line includes cookie, cake and loaf pans with grips, novelty bakeware like the signature flower cakelett pan; specialty baking utensils like a spoonula, a mechanical cookie scoop and a balloon whisk; and accessories like decorating tip sets, a fondant ribbon cutter, a letter impression set, and specialty fondant acrylic rolling pins.
Now you can have your cake and eat it with candy, too. Tastycake and Hershey's have teamed up to create Kandy Bar Kakes — part perfectly baked cake, part candy, made with quality ingredients.
Kandy Bar Kakes are available in 2.1-oz. single-serve sizes and 5-count multipacks in three varieties: s'mores, made with Hershey's Cocoa; peanut butter, made with Reese's peanut butter; and peppermint, made with York Peppermint flavor.


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What does NMSQT stand for?
What does NMSQT mean? This page is about the various possible meanings of the acronym, abbreviation, shorthand or slang term: NMSQT.
We've found a total of 1 definition for NMSQT:
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NMSQT | National Merit Scholarship Qualifying Test |
Still can't find the acronym definition you were looking for? Use our Power Search technology to look for more unique definitions from across the web!
"NMSQT." Abbreviations.com. STANDS4 LLC, 2015. Web. 25 Jan. 2015. <http://www.abbreviations.com/NMSQT>.


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Welcome to DBSTalk
Like most online communities you must register to view or post in our community. Sign-up is a free and simple process that requires minimal information. Be a part of our community by signing in or creating an account. The Digital Bit Stream starts here!
- Reply to existing topics or start a discussion of your own
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Genie HR44 UPgrade
Posted 12 August 2013 - 05:31 PM
I received the SWM LNB from Amazon.com today, and hooked it up to my HR-22 and HR-24. Had to go with the complete satellite setups again, but didn't take too long. Everything working perfectly, so ready for the Genie. Sure hope I get a new HR-44 and don't have to arm wrestle with them.
...Ads Help To Support This Site... |
Posted 18 April 2014 - 10:29 AM
I came across your post recently. I have an almost identical situation to you - I currently have an HR20 and HR24 in 2 rooms, and I'd like to upgrade to Genie. I also have an old lifetime DVR subscription (from old Tivo) - there's not many of us who have this, DirecTv once told me less than 200! I'm also a subscriber since 1994. Its always been a bit of an effort to work with upgrades and make sure they keep my lieftime DVR subscription. What did you find out for the costs from DirecTV? Did they give you any break on the monthly fees?
Posted 22 April 2014 - 10:34 AM
Did they give you any break on the monthly fees?
They don't usually discount monthly fee's like $6/receiver, or the MRV fee, but occasionally they will credit you some back for the HD fee's....


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Welcome to LACMA's online press room.
Below, you can access recent news and press releases related to the museum's current and upcoming exhibitions, collections, programs and other major news. To retrieve images and other related press materials, please register a press account with us below. If you have registered previously for our Image Bank, please use that log-in information or access it by using the "Forgot password" function.
All press materials are provided by LACMA's Communications Department. If you have questions regarding public relations, image requests, or would like to be added to our mailing list, please contact us at 323 857-6522 or via email.
Recent News & Press Releases
LACMA is pleased to announce eight promised gifts of art from Jane and Marc Nathanson. The Nathansons' gift of eight works of contemporary art includes seminal pieces by Damien Hirst, Roy Lichtenstein, Frank Stella, Andy Warhol, and others.
The bequest is made in honor of LACMA's 50th anniversary in 2015. The gifts kick off a campaign, chaired by LACMA trustees Jane Nathanson and Lynda Resnick, to encourage additional promised gifts of art for the museum's anniversary. Gifts resulting from this campaign will be exhibited at LACMA April 26–September 7, 2015, in an exhibition, 50 for 50: Gifts on the Occasion of LACMA's 50th Anniversary.
Access more press materials by subject.


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Thanks for great service and very prompt shipping. Items took only 4 days to get over to here in New Zealand! Thanks againRead More ->
Wow seriously you guys are like God. I live in Canada and I bought something on Tuesday...Bam! It came the following Thursday all packaged. Thx...Read More ->


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A multitude of Vista tweaks
Find endless options for customizing your Vista settings with this free utility, but it lacks password-protection and a useful Help feature.
The options for customizing your Vista settings are endless with this free utility, but it lacks password-protection and a useful Help feature.
WinBubble has so many tweaking options that it could easily overwhelm users. The user interface uses tabs and check boxes to keep the multitude of options organized and easy to activate. The program lets you add more options to your context menu, including a Safely Remove Hardware icon, an encrypt/decrypt option, and a WinBubble icon. The Tweaking Toolbox clears your cache and resets folder views. You can even customize icons and add a custom screensaver. Security options are also plentiful and let you apply administrator alerts, disable Internet Explorer features, and even hide specific drives. Most of the tweaks that we applied took immediate effect, and others required either a log off or restart the computer. The main drawback to the program is that it isn't password-protected. That means that anyone can go in and change your settings. We were also disappointed to find that it doesn't come with a Help menu. Rather, you have to visit the publisher's site, which holds only a blog that doesn't offer much information.
Thanks to the easy-to-use design, though, even novices should have no problem figuring out how to use the program. We highly recommend it for Vista users looking for an easy way to tweak their machines.


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US 7723020 B2 Abstract The use of azetidinone compounds that are inhibitors of cholesterol absorption as tools for discovering and characterizing proteins involved in trafficking or absorption of cholesterol and/or cholesteryl esters in biological systems is presented. These compounds can serve as tools for competitive binding assays to discover and characterize other chemical agents useful as cholesterol absorption inhibitors. New compounds of the present invention are also highly efficacious inhibitors of cholesterol absorption.
Claims(6) 1. An isolated cell wherein a fluorescent cholesterol absorption inhibitor is bound to a surface of said cell, wherein said inhibitor is an azetidinone.
3. The cell of
5. The cell of
6. The cell of
Description This application is a continuation of U.S. application Ser. No. 11/1123,615, filed May 6, 2005, now U.S. Pat. No. 7,144,696, which is a divisional of U.S. application Ser. No. 10/438,637, filed May 15, 2003, now U.S. Pat. No. 6,933,107, which is a divisional of U.S. application Ser. No. 09/547,509, filed Apr. 12, 2000, now U.S. Pat. No. 6,593,078, which claims priority under 35 U.S.C. �119(e) to U.S. Application Ser. No. 60/129,610, filed Apr. 16, 1999, now abandoned, each of which are herein incorporated by reference in their entireties. The present invention relates to the use of azetidinone compounds that are inhibitors of cholesterol absorption as tools for discovering and characterizing proteins involved in trafficking or absorption of cholesterol and/or cholesteryl esters in biological systems. Further, these compounds can serve as tools for competitive binding assays to discover and characterize other chemical agents useful as cholesterol absorption inhibitors. New compounds of the present invention are highly efficacious inhibitors of cholesterol absorption. Atherosclerotic coronary heart disease (CHD) represents the major cause for death and cardiovascular morbidity in the western world. Risk factors for CHD include hypertension, diabetes mellitus, family history, male gender, cigarette smoke and serum cholesterol. A total cholesterol level in excess of 225 to 250 mg/dl is associated with significant elevation of risk of CHD. Cholesteryl esters are a major component of atherosclerotic lesions and the major storage form of cholesterol in arterial wall cells. Formation of cholesteryl esters is also a key step in the intestinal absorption of dietary cholesterol. Thus, inhibition of cholesteryl ester formation and reduction of serum cholesterol is likely to inhibit the progression of atherosclerotic lesion formation, decrease the accumulation of cholesteryl esters in the arterial wall, and block the intestinal absorption of dietary cholesterol. The regulation of whole-body cholesterol homeostasis in humans and animals involves the regulation of dietary cholesterol and modulation of cholesterol biosynthesis, bile acid biosynthesis and the catabolism of the cholesterol-containing plasma lipoproteins. The liver is the major organ responsible for cholesterol biosynthesis and catabolism and for this reason, it is a prime determinant of plasma cholesterol levels. The liver is the site of synthesis and secretion of very low density lipoproteins (VLDL) which are subsequently metabolized to low density lipoproteins (LDL) in the circulation. LDL are the predominant cholesterol-carrying lipoproteins in the plasma and an increase in their concentration is correlated with increased atherosclerosis. When intestinal cholesterol absorption is reduced, by whatever means, less cholesterol is delivered to the liver. The consequence of this action is decreased hepatic lipoprotein production, and an increase in the hepatic clearance of plasma cholesterol, mostly as LDL. Thus, the net effect of inhibiting intestinal cholesterol absorption is a decrease in plasma cholesterol levels. Certain azetidinone core structures have been reported to be useful in lowering cholesterol levels by decreasing intestinal cholesterol absorption. These related azetidinone cores and their synthesis are detailed in the following commonly assigned U.S. patents, the disclosures of which are incorporated, in their entirety, herein by reference: U.S. Pat. Nos. 5,688,787; 5,698,548; 5,624,920; 5,631,365; 5,633,246; 5,656,624; 5,744,467; and 5,767,115. The discovery of 2-azetidinones as potent and selective intestinal cholesterol absorption inhibitors has confirmed this mechanism as a key point of intervention for lowering cholesterol plasma levels and has validated the therapeutic value of such an approach. The mechanism by which cholesterol moves from the lumen into the epithelial layer lining the small intestine is not well understood. Recent experimental evidence supports the notion of an active transport process mediated by a protein or proteins in the enterocyte brush border membrane rather than a simple diffusion model. Kinetic analysis and sterol specificity of cholesterol uptake as well as the structure-activity relationship studies of the cholesterol absorption inhibitors are consistent with a specific protein receptor/transporter regulated event. Potential molecules for this process have been proposed in recent years. However, the specific biochemical pathway responsible for cholesterol absorption remains to be defined. Compounds I and II (especially II) In one aspect, this invention provides a method for identifying a protein involved in cholesterol absorption in a biological system, the method comprising the steps of: (a) providing a cDNA expression library capable of expressing a protein involved in cholesterol absorption in a biological system, said cDNA expression library comprising a plurality of cells capable of expressing different cDNAs; (b) screening said expression library by incubating cells from said library with a fluorescent cholesterol absorption inhibitor; (c) after step (b), identifying the cell or cells in said library that display the greatest amount of fluorescence; and (d) identifying the protein associated with the cDNA expressed by said cell or cells displaying the greatest amount of fluorescence. In another aspect, this invention provides a method for assaying inhibitory agents for activity against cholesterol absorption, the method comprising the steps of: providing a cell capable of binding a fluorescent cholesterol absorption inhibitor; contacting said cell with a candidate inhibitory agent in the presence of said fluorescent cholesterol absorption inhibitor; and measuring the inhibition of the fluorescence of said cell. In still another aspect, this invention provides a method for identifying inhibitory agents which inhibit the absorption of cholesterol into a cell membrane, said method comprising the steps of: (a) combining a fluorescent cholesterol absorption inhibitor, said cell membrane and a candidate inhibitory agent, under conditions wherein, but for the presence of said inhibitory agent, said fluorescent cholesterol absorption inhibitor is bound to the membrane; and (b) detecting the relative presence or absence of fluorescent cholesterol absorption inhibitor absorption bound to the membrane, wherein a relative absence of fluorescent cholesterol absorption inhibitor absorption indicates that said candidate inhibitory agent is an inhibitory agent which inhibits cholesterol absorption into the membrane. In another aspect, this invention provides a method for identifying inhibitory agents which inhibit the absorption of cholesterol, said method comprising the steps oft (a) combining a labeled cholesterol absorption inhibitor, a cell expressing the scavenger receptor type B, class I (SR-SI) and a candidate inhibitory agent, under conditions wherein, but for the presence of said inhibitory agent, said labeled cholesterol absorption inhibitor binds to SR-BI; and (b) detecting the relative presence or absence of labeled cholesterol absorption inhibitor absorption bound to SR-BI wherein a relative absence of labeled cholesterol absorption inhibitor absorption indicates that said candidate inhibitory agent is an inhibitory agent which inhibits SR-BI-mediated cellular cholesterol absorption. In further aspects, this invention provides proteins and new inhibitory agents identified by the above methods. This invention also provides novel fluorescent cholesterol absorption inhibitors of formulas I and II:
wherein R comprises a fluorescent moiety. A. Novel Fluorescent Cholesterol Absorption Inhibitors Novel fluorescent cholesterol absorption inhibitors of this invention include compounds of formulas I and II:
wherein R comprises a fluorescent moiety. In preferred embodiments, R is a fluorescent moiety linked by an alkynyl-containing tether group. In a particularly preferred embodiment, R is selected from the group consisting of:
Modification with fluorescent moieties A-B of the N-aryl ring of other azetidinone cholesterol absorption inhibitor core structures related to the above are contemplated as being within the scope of this invention as well. These related azetidinone cores and their synthesis are detailed in the following commonly assigned U.S. patents the disclosures of which are incorporated, in their entirety, herein by reference: U.S. Pat. Nos. 5,688,787; 5,698,548; 5,624,920; 5,631,365; 5,633,246; 5,656,624; 5,744,467; and 5,767,115. Compounds in the following text are designated as:
The synthetic route to these fluorescent azetidinones is shown in Scheme 1. The fluorescent alkynyl compounds are prepared from commercially available electrophilic fluorescent derivatives (see Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Inc.) and propargyl amine. The syntheses of N-iodophenyl azetidinone derivatives have been described in previous patents, cited above. The fluorescent alkynylated material is then coupled to the N-iodophenylazetidinone via a palladium mediated coupling reaction Deprotection of the glucuronide ester is accomplished as a final step in analogs bearing this moiety. The practice of the present invention generally employs conventional techniques of molecular biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See for example J. Sambrook et al, �Molecular Cloning; A Laboratory Manual (1989); �DNA Cloning�Vol. I and II (D. N. Clover ed. 1985); �Oligonucleotide Synthesis� (M. J. Gait ed, 1984); �Nucleic Acid Hybridization� (S. D. Hames & S. J. Higgins eds. 1984); �Transcription And Translation� (B. D. Hames & S. J. Higgins eds. 1984); �Animal Cell Culture� (R. I. Freshney ed. 1986); �Immobilized Cells And Enzymes� (IRL Press, 1986); B. Perbal, �A Practical Guide To Molecular Cloning� (1984); the series, �Methods In Enzymology� (Academic Press, Inc.); �Gene Transfer Vectors For Mammalian Cells� (J. H. Miller and M. P. Calos eds. 1987, Cold Spring Harbor Laboratory); Meth Enzymol (1987) 154 and 155 (Wu and Grossman, and Wu, eds., respectively); Mayer & Walker, eds. (1987), �Immunochemical Methods In Cell And Molecular Biology� (Academic Press, London); Scopes, �Protein Purification: Principles And Practice�, 2nd Ed (Springer-Verlag, N.Y., 1987), and �Handbook Of Experimental Immunology�, volumes I-IV (Weir and Blackwell, eds, 1986). C. Methods of Screening cDNA Libraries cDNA libraries containing genes capable of expressing a protein involved in cholesterol absorption are prepared by standard techniques, such as described in the above references. Both prokaryotic and eukaryotic host cells are useful for expressing desired coding sequences when appropriate control sequences compatible with the designated host are used. Among prokaryotic hosts, E. coli is most frequently used. Expression control sequences for prokaryotes include promoters, optionally containing operator portions, and ribosome binding sites. Transfer vectors compatible with prokaryotic hosts are commonly derived from, for example, pBR322, a plasmid containing operators conferring ampicillin and tetracycline resistance, and the various pUC vectors, which also contain sequences conferring antibiotic resistance markers. These plasmids are commercially available. The markers may be used to obtain successful transformants by selection. Commonly used prokaryotic control sequences include the β-lactamase (penicillinase) and lactose promoter systems (Chang et al, Nature (1977) 198:1056), the tryptophan (trp) promoter system (Goeddel et al, Nuc Acids Res (1980) 8:4057) and the lambda-derived PL promoter and N gene ribosome binding site (Shimatake et al, Nature (1981) 292:128) and the hybrid tac promoter (De Boer et al, Proc Nat Acad Sci USA (1983) 292:128) derived from sequences of the trp and lac UV5 promoters. The foregoing systems are particularly compatible with E. coli; if desired, other prokaryotic hosts such as strains of Bacillus or Pseudomonas may be used, with corresponding control sequences. Eukaryotic hosts include without limitation yeast and mammalian cells in culture systems. Yeast expression hosts include Saccharomyces, Klebsiella, Picia, and the like. Saccharomyces cerevisiae and Saccharomyces carlsbergensis and K. lactis are the most commonly used yeast hosts, and are convenient fungal hosts. Yeast-compatible vectors carry markers which permit selection of successful transformants by conferring prototrophy to auxotrophic mutants or resistance to heavy metals on wild-type strains. Yeast compatible vectors may employ the 2 μ origin of replication (Broach et al, Meth Enzymol (1983) 101:307), the combination of CEN3 and ARS1 or other means for assuring replication, such as sequences which will result in incorporation of an appropriate fragment into the host cell genome. Control sequences for yeast vectors are known in the art and include promoters for the synthesis of glycolytic enzymes (Hess et al, J Adv Enzyme Reg (1968) 7:149; Holland et al, Biochem (1978), 17:4900), including the promoter for 3-phosphoglycerate kinase (R. Hitzeman et al, J Biol Chem (1980) 255:2073). Terminators may also be included, such as those derived from the enolase gene (Holland, J Biol Chem (1981) 256:1385). Particularly useful control systems are those which comprise the glyceraldehyde-3 phosphate dehydrogenase (GAPDH) promoter or alcohol dehydrogenase (ADH) regulatable promoter, terminators also derived from GAPDH, and if secretion is desired, a leader sequence derived from yeast alpha-factor (see U.S. Pat. No. 4,870,008, incorporated herein by reference). In addition, the transcriptional regulatory region and the transcriptional initiation region which are operably linked may be such that they are not naturally associated in the wild-type organism. These systems are described in detail in EPO 120,551, published Oct. 3, 1984; EPO 116,201, published Aug. 22, 1984; and EPO 164,556, published Dec. 18, 1985, all of which are hereby incorporated herein by reference in full. Mammalian cell lines available as hosts for expression are known in the art and include many immortalized cell lines available from the American Type Culture Collection (ATCC), including HeLa cells, Chinese hamster ovary (CHO) cells, baby hamster kidney (BHK) cells, and a number of other cell lines. Suitable promoters for mammalian cells are also known in the art and include vital promoters such as that from Simian Virus 40 (SV40) (Fiers et al, Nature (1978) 273:113), Rous sarcoma virus (RSV), adenovirus (ADV), and bovine papilloma virus (BPV). Mammalian cells may also require terminator sequences and poly-A addition sequences. Enhancer sequences which increase expression may also be included, and sequences which promote amplification of the gene may also be desirable (for example methotrexate resistance genes). These sequences are known in the art. Vectors suitable for replication in mammalian cells are known in the art, and may include vital replicons, or sequences which insure integration of the appropriate sequences into the host genome. For example, another vector used to express foreign DNA is Vaccinia virus. In this case the heterologous DNA is inserted into the Vaccinia genome. Techniques for the insertion of foreign DNA into the vaccinia virus genome are known in the art, and may utilize, for example, homologous recombination. The heterologous DNA is generally inserted into a gene which is non-essential to the virus, for example, the thymidine kinase gene (tk), which also provides a selectable marker. Plasmid vectors that greatly facilitate the construction of recombinant viruses have been described (see, for example, Mackett et al, J Virol (1984) 49:857; Chakrabarti et al, Mol Cell Biol (1985) 5:3403; Moss, in GENE TRANSFER VECTORS FOR MAMMALIAN CELLS (Miller and Calos, eds., Cold Spring Harbor Laboratory, N.Y., 1987), p. 10). Expression of the polypeptide then occurs in cells or animals which are infected with the live recombinant vaccinia virus. Transformation may be by any known method for introducing polynucleotides into a host cell, including, for example packaging the polynucleotide in a virus and transducing a host cell with the virus, and by direct uptake of the polynucleotide. The transformation procedure used depends upon the host to be transformed. Bacterial transformation by direct uptake generally employs treatment with calcium or rubidium chloride (Cohen, Proc Nat Acad Sci USA (1972) 69:2110; T. Maniatis et at, �Molecular Cloning; A Laboratory Manual� (Cold Spring Harbor Press, Cold Spring Harbor, N.Y., 1982). Yeast transformation by direct uptake may be carried out using the method of Hinnen et al, Proc Nat Acad Sci USA (1978) 75:1929. Mammalian transformations by direct uptake may be conducted using the calcium phosphate precipitation method of Graham and Van der Eb, Virol (1978) 52:546, or the various known modifications thereof. Other methods for introducing recombinant polynucleotides into cells, particularly into mammalian cells, include dextran-mediated transfection, calcium phosphate mediated transfection, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, and direct microinjection of the polynucleotides into nuclei. Vector construction employs techniques which are known in the art, Site-specific DNA cleavage is performed by treating with suitable restriction enzymes under conditions which generally are specified by the manufacturer of these commercially available enzymes. In general, about 1 μg of plasmid or DNA sequence is cleaved by 1 unit of enzyme in about 20 μL buffer solution by incubation for 1-2 hr at 37� C. After incubation with the restriction enzyme, protein is removed by phenol/chloroform extraction and the DNA recovered by precipitation with ethanol. The cleaved fragments may be separated using polyacrylamide or agarose gel electrophoresis techniques, according to the general procedures described in Meth Enzymol (1980) 65:499-560. Sticky-ended cleavage fragments may be blunt ended using E. coli DNA polymerase I (Klenow fragment) with the appropriate deoxynucleotide triphosphates (dNTPs) present in the mixture. Treatment with S1 nuclease may also be used, resulting in the hydrolysis of any single stranded DNA portions. Ligations are carried out under standard buffer and temperature conditions using T4 DNA ligase and ATP; sticky end ligations require less ATP and less ligase than blunt end ligations. When vector fragments are used as part of a ligation mixture, the vector fragment is often treated with bacterial alkaline phosphatase (BAP) or calf intestinal alkaline phosphatase to remove the 5′-phosphate, thus preventing religation of the vector. Alternatively, restriction enzyme digestion of unwanted fragments can be used to prevent ligation. Ligation mixtures are transformed into suitable cloning hosts, such as E. coli, and successful transformants selected using the markers incorporated (e.g., antibiotic resistance), and screened for the correct construction. Synthetic oligonucleotides may be prepared using an automated oligonucleotide synthesizer as described by Warner, DNA (1984) 3:401. If desired, the synthetic strands may be labeled with 32P by treatment with polynucleotide kinase in the presence of 32P-ATP under standard reaction conditions. For routine vector constructions, ligation mixtures are transformed into E. coli strain HB101 or other suitable hosts, and successful transformants selected by antibiotic resistance or other markers. Plasmids from the transformants are then prepared according to the method of Clewell et al, Proc Nat Acad Sci USA (1969) 62:1159, usually following chloramphenicol amplification (Clewell, J Bacteriol (1972) 110:667). The DNA is isolated and analyzed, usually by restriction enzyme analysis and/or sequencing. Sequencing may be performed by the dideoxy method of Sanger et at, Proc Nat Acad Sci USA (1977) 74:5463, as further described by Messing et at, Nuc Acids Res (1981) 9:309, or by the method of Maxam et at, Meth Enzymol (1980) 65:499. Problems with band compression, which are sometimes observed in GC-rich regions, were overcome by use of T-deazoguanosine according to Barr et al, Biotechniques (1986) 4:428. Preferably, the cDNA libraries used in this invention are derived from the mRNA of cells or cell lines known to be involved in cholesterol absorption. In a preferred embodiment of this invention, the cDNA library is derived from the mRNA of an intestinal epithelial cell. The cDNA libraries are then screened by incubating cells from the library with a fluorescent cholesterol absorption inhibitor, and identifying those cells that display the greatest amount of fluorescence, using, for example, fluorescence activated cell sorting (FACS). The cDNA expressed in that cell can then be identified using known techniques. D. Methods of Screening for Inhibitory Agents New cholesterol absorption inhibitory agents are screened using methods of the invention. In general, a substrate is employed which mimics the protein's natural substrate, but which provides a quantifiable signal when bound. The signal is preferably detectable by colorimetric or fluorometric means: however, other methods such as HPLC or silica gel chromatography, GC-MS, nuclear magnetic resonance, and the like may also be useful. After optimum substrate and protein (or membrane or cell) concentrations are determined, a candidate inhibitory agent is added to the reaction mixture at a range of concentrations. The assay conditions ideally should resemble the conditions under which cholesterol absorption is to be inhibited in vivo, i.e., under physiologic pH, temperature, ionic strength, etc. Suitable inhibitors will exhibit absorption inhibition at concentrations which do not raise toxic side effects in the subject. Inhibitors which compete for binding may require concentrations equal to or greater than the substrate concentration, while inhibitors capable of binding irreversibly to the cholesterol binding site may be added in concentrations on the order of the protein concentration. This invention provides several methods for assaying, identifying or screening inhibitory agents for activity against cholesterol absorption, including cellular techniques, membrane techniques, and techniques using proteins that are involved in cholesterol absorption, such as the one provided by the current invention, the scavenger receptor type B, class I (SR-BI). SR-BI was first described by Acton et al., J. Biol. Chem. (1994) 269:21003-21009. Screening for inhibitory agents is accomplished as described below. In the cellular method, cells capable of binding a fluorescent cholesterol absorption inhibitor are used. In a preferred embodiment, the cells are involved in cholesterol absorption in a biological system. The cells are contacted with candidate inhibitory agents in the presence of the fluorescent cholesterol absorption inhibitor; the inhibition of the fluorescence of said cells are measured. In the membrane method, a membrane capable of absorbing cholesterol is used. In a preferred embodiment of this invention, the membranes are from the intestinal brush border. The membranes are contacted with candidate inhibitory agents in the presence of the fluorescent cholesterol absorption inhibitor; and the relative presence or absence of fluorescent cholesterol absorption inhibitor absorption bound to the membrane is measured. A relative absence of fluorescent cholesterol absorption inhibitor absorption indicates that said candidate inhibitory agent is an inhibitory agent which inhibits cholesterol absorption into the membrane. In the protein method, a cell expressing the scavenger receptor type B, class I (SR-BI) is used. The cell is contacted with a labeled cholesterol absorption inhibitor and a candidate inhibitory agent under conditions wherein, but for the presence of said inhibitory agent, the labeled cholesterol absorption inhibitor binds to SR-BI, and the relative presence or absence of labeled cholesterol absorption inhibitor absorption bound to SR-BI is detected. A relative absence of labeled cholesterol absorption inhibitor absorption indicates that the candidate inhibitory agent is an inhibitory agent which inhibits SR-BI-mediated cellular cholesterol absorption. The methods of assaying or selecting inhibitory agents of this invention use labeled cholesterol absorption inhibitors. The inhibitors themselves are described in section A, above. The labels used in the assays of invention can be primary labels (where the label comprises an element which is detected directly) or secondary labels (where the detected label binds to a primary label, e.g., as is common in immunological labeling). An introduction to labels, labeling procedures and detection of labels is found in Polak and Van Noorden (1997) Introduction to Immunocytochemistry, second edition, Springer Verlag, N.Y. and in Haugland (1996) Handbook of Fluorescent Probes and Research Chemicals, a combined handbook and catalogue Published by Molecular Probes, Inc., Eugene, Oreg. Primary and secondary labels can include undetected elements as well as detected elements. Useful primary and secondary labels in the present invention can include spectral labels, which include fluorescent labels such as fluorescent dyes (e.g., fluorescein and derivatives such as fluorescein isothiocyanate (FITC) and Oregon Green�, rhodamine and derivatives (e.g., Texas red, tetramethylrhodamine isothiocyanate (TRITC), etc.), digoxigenin, biotin, phycoerythrin, AMCA, CyDyes�, and the like), radiolabels (e.g., 3H, 125I, 35S, 14C, 32P, 33p, etc.), enzymes (e.g., horseradish peroxidase, alkaline phosphatase etc.) spectral colorimetric labels such as colloidal gold or colored glass or plastic (e.g. polystyrene, polypropylene, latex, etc.) beads. The label may be coupled directly or indirectly to the cholesterol absorption inhibitor according to methods well known in the art. As indicated above, a wide variety of labels may be used, with the choice of label depending on sensitivity required, ease of conjugation with the compound, stability requirements, available instrumentation, and disposal provisions. In general, a detector which monitors a protein/inhibitory agent interaction is adapted to the particular label which is used. Typical detectors include spectrophotometers, phototubes and photodiodes, microscopes, scintillation counters, cameras, film and the like, as well as combinations thereof. Examples of suitable detectors are widely available from a variety of commercial sources known to persons of skill. Preferred labels include those which utilize 1) chemiluminescence (using horseradish peroxidase or alkaline phosphatase with substrates that produce photons as breakdown products) with kits being available, e.g., from Molecular Probes, Amersham, Boehringer-Mannheim, and Life Technologies/Gibco BRL; 2) color production (using both horseradish peroxidase or alkaline phosphatase with substrates that produce a colored precipitate) (kits available from Life Technologies/Gibco BRL, and Boehringer-Mannheim); 3) fluorescence (e.g., using Cy-5 (Amersham), fluorescein, and other fluorescent tags); 5) radioactivity. Other methods for labeling and detection will be readily apparent to one skilled in the art. Fluorescent labels are highly preferred labels, having the advantage of requiring fewer precautions in handling, and being amendable to high-throughput visualization techniques (optical analysis including digitization of the image for analysis in an integrated system comprising a computer). Preferred labels are typically characterized by one or more of the following: high sensitivity, high stability, low background, low environmental sensitivity and high specificity in labeling. Fluorescent moieties, which are incorporated into the labels of the invention, are generally are known, including Texas red, digoxigenin, biotin, 1- and 2-aminonaphthalene, p,p′-diaminostilbenes, pyrenes, quaternary phenanthridine salts, 9-aminoacridines, p,p′-diaminobenzophenone imines, anthracenes, oxacarbocyanine, merocyanine, 3-aminoequilenin, perylene, bis-benzoxazole, bis-p-oxazolyl benzene, 1,2-benzophenazin, retinol, bis-3-aminopyridinium salts, hellebrigenin, tetracycline, sterophenol, benzimidazolylphenylamine, 2-oxo-3-chromen, indole, xanthen, 7-hydroxycoumarin, phenoxazine, calicylate, strophanthidin, porphyrins, triarylmethanes, flavin and many others. Many fluorescent tags are commercially available from the SIGMA chemical company (Saint Louis, Mo.), Molecular Probes, R&D systems (Minneapolis, Minn.), Pharmacia LKB Biotechnology (Piscataway, N.J.), CLONTECH Laboratories, Inc. (Palo Alto, Calif.), Chem Genes Corp., Aldrich Chemical Company (Milwaukee, Wis.), Glen Research, Inc., GIBCO BRL Life Technologies, Inc. (Gaithersberg, Md.), Fluka ChemicaBiochemika Analytika (Fluka Chemie AG, Buchs, Switzerland), and Applied Biosystems (Foster City, Calif.), as well as many other commercial sources known to one of skill. The labels are covalently bound to the novel cholesterol inhibitors of the invention by a tether group. The tether group can be any moiety capable of covalently linking to the inhibitors and to the labels. Preferred groups are substituted or unsusbstituted alkylene, alkenylene or alkynylene of 1 to 10 carbon atoms, more preferably 1 to 4 carbon atoms. Particularly preferred groups are unsusbstituted alkynylenes. The candidate and actual inhibitory agents of this invention will preferably take the form of organic compounds, particularly compounds which inhibit the absorption of cholesterol mediated by SR-BI and other proteins3. Other inhibitory agents may be proteins, particularly antibodies and antibody derivatives. Recombinant expression systems may be used to generate quantities of SR-BI sufficient for production of monoclonal antibodies (MAbs) specific for SR-BI. Suitable antibodies for cholesterol absorption inhibition will bind to SR-BI in a manner reducing or eliminating the cholesterol binding activity, typically by obscuring the binding site. Suitable MAbs may be used to generate derivatives, such as Fab fragments, chimeric antibodies, altered antibodies, univalent antibodies, and single domain antibodies, using methods known in the art.
Added propargyl amine (5 μL,�4 mg, 73 μmol) to 0.3 mL DMF containing 3-5 drops 0.2M aqueous NaHCO,3then added 6-(Fluorescein-5-carboxamido)hexanoic acid, succinimidyl ester (5-SFX; Molecular Probes, Inc.) (5 mg, 8.5 μmol) to the mixture. Stirred the bright orange solution for 3 h at room temperature. Concentrated in vacuo and chromatographed the residue over �5 g of silica gel eluting with 10% MeOH in dichloromethane to give the desired terminal alkyne (1) as a yellow brown fluorescent oil. calcd m/z for C30H27N2O7=527; found m/z=527.
To the iodophenylazetidinone glucuronide methyl ester IIIA (disclosed in Pat. No. 5,756,470, May 26, 1998)( 6.7 mg, 9.47 μmol) in 0.8 mL DMF, was added the alkyne (1) (14.8 mg, 28 μmol). Bubbled argon through the reaction mixture, then added palladium tetrakistriphenylphosphine (1.4 mg, 1.2 μmol), copper(I) iodide (0.8 mg, 4.2 μmol), and triethylamine (8 μL, 57 μmol). Stirred the reaction mixture overnight under argon. Concentrated the reaction onto a prep TLC plate and eluted with 25% MeOH in dichloromethane. Collected the major fluorescent band and purified further by reverse phase HPLC over aC-18 column eluting with 10-100% acetonitrile in water affording the desired fluorescent methyl ester as an orange solid. FABMS: calcd m/z for C61H57N3FO16 (M+1)+1106.4; found m/z=1106.5. The glucuronide methyl ester was then hydrolyzed by dissolving 9 mg of the compound in 2 mL of a 7:2:1 mixture of water:triethylamine: methanol and stirring for 1 h. Evaporation to dryness gave the desired fluorescent azetidinone glucuronide, (IB) as an orange solid. ES-MS: calcd m/z for C60H55N3FO16 (M+1)+=1092.3; found m/z=-1092.3.
To propargyl amine (8 μL, �6.4 mg, -117 μmol) in 0.5mL DMF and 1 drop of 0.1M NaHCO3, was added 4,4-difluoro-5,7-dimethyl4-bora-3a,4a-diaza-s-indacene-3-propionic acid, succinimide ester (BODIPY FL, SE; Molecular Probes, In.) (10 mg, 25.7 μmol) in 0.1mL DMF. Stirred the reaction at room temperature for 12 h. Concentrated the mixture in vacuo and chromatographed the residue over �5 g silica gel eluting with 1% MeOH in dichloromethane to give the desired alkyne (2) as a yellow orange oil. Rf=0.62 in 5% MeOH in CH2Cl2 on SiO2 TLC.
To the iodophenylazetidinone glucuronide methyl ester IIIA (disclosed in Pat. No. 5756,470 May 26, 1998) (7.7 mg, 10.9 μmol) in 1 mL DMF, was added (7.2 mg, 10.9 μmol) of the BODIPY alkyne (2). Bubbled argon through the solution for a few minutes and then added palladium tetrakistriphenylphosphine (1.2 mg, 1 μmol), copper(I) iodide (0.6 mg, 3 μmol), and triethylamine (10 μL, 7 μmol). Stirred the reaction overnight under argon. Removed the solvent in vacuo and purified the product via prep TLC eluting with a 50:50:17:1 mixture of ethyl acetate:hexanes:methanol:acetic acid to give the desired fluorescent methyl ester as an orange solid. ESI-MS: calcd m/z for C48H49BF3N4O10 (M+1)+=909.3; found m/z=909.2. The glucuronide methyl ester was then hydrolyzed by dissolving 4.2 mg of the compound in �1 mL of a 7:2:1 mixture of water:triethylamine: methanol and stirring for 1 h. Evaporation to dryness gave the desired fluorescent azetidinone glucuronide (IC) as an orange solid, ES-MS: calcd m/z for C47H46BF2N4O10 (M+1−HF)+=875.3; found m/z=875.3. Male Sprague-Dawley rats weighing 300-400 g were used. After an overnight fast, rats were anesthetized (Inactin, 0.1 mg/kg i.p.) for the duration of each study and were fitted with a cannula into the small intestine just below the pyloric valve. For the cannulation of the small intestine, a catheter (Surflo� i.v. catheter (18GX2″), Terumo Medical Corporation; Elkton, Md.) was inserted through the fundus of the stomach, advanced 1 cm beyond the pylorus, and ligated in place. Compounds were mixed in rat bile and delivered by bolus injection (1 mL) via the intestinal catheter into the small intestine. One hour after the bile doses were delivered, three mL of an emulsion consisting of 2.23 mg/mL L-phosphatidylcholine and 11.8 mg/mL triolein in 19 mM sodium taurocholate (Sigma; St. Louis, Mo.) buffer (pH 6.4) containing 3 mg cholesterol and 1 mCi 14C-cholesterol (NEN; Boston, Mass.) was delivered to each rat as a bolus via the intestinal cannula. Ninety minutes after the cholesterol emulsion was delivered, the rats were euthanized. Blood was collected and plasma was separated by centrifugation at 2000 rpm for 15 min at 4� C. Triplicate aliquots of plasma were analyzed 14C radioactivity and inhibition of cholesterol absorption relative to bile vehicle control rats was determined. The ID50 for IIA in this acute model of cholesterol absorption was calculated to be 0.0015 mg/kg (Van Heek, M., France, C. F., Compton, D. S., McLeod, R. L., Yumibe, N. P., Alton, K. B., Sybertz, E. J., and Davis, H. R.: In vivo mechanism-based discovery of a potent cholesterol absorption inhibitor (IIA) through the identification of the active metabolites of IVA. The Journal of Pharmacology and Experimental Therapeutics (JPET), 283: 157-163, 1997).
The small intestine was removed from male CRL: CD BR rats. Intestinal epithelial cells were isolated by the method of Weiser (Weiser, M., 1973, JBC 248, 2536-2541) and the mRNA extracted. Poly A mRNA was purified and double stranded cDNA synthesized. Adapter linkers were ligated followed by size selection (>2 Kb) of the cDNA. The selected fraction of cDNA was ligated into the retroviral expression plasmid pMX, subsequently transformed into bacteria and grown on selective agar at a clonal density of −30,000/plate. Each pool of bacterial clones was collected and plasmid DNA prepared. Individual pools of cDNA library were transformed into the retroviral packaging cell line BOSC23 and the resulting viral particles, containing the cDNA library, were used to infect the mouse cell line BW5147. Following infection BW5147 cells were stained with compound 1C and analyzed by fluorescent activated cell sorting (FACS). Positive staining cells (−01%) were recovered and re-cultured. The process of recovering positive staining cells was repeated after expansion of the cell population in culture and following the second round of FACS analysis individual cells were isolated by limiting dilution. Individual clonal populations of cells were subjected to PCR using retroviral vector specific oligonucleotide primers designed to flank the multiple cloning site in which the cDNA library was cloned. Resulting DNA fragments were subcloned and completely sequenced to determine the identification of the cloned cDNA. By sequencing two clones isolated from two independent cDNA expression libraries, Scavenger receptor, class B, type I (SR-BI) (GENBANK Accession Numbers: U76205 �submission date Oct. 24, 1996; D89655�submission date Dec. 3, 1996, and AB002151�submission date Mar. 26, 1997) was identified as the cellular receptor for compound IC. The above described method may be used in conjunction with any of the azetidinone compounds referred to herein to identify other proteins that are involved in cholesterol absorption or trafficking. Once identified, further characterization of the proteins can be conducted, for example, using the following methods: (1) FACS analysis of target expressing cells with fluorescent compound binding can be utilized in both direct and competition binding formats. Added complexity by the addition of other cellularly expressed proteins as well as the addition of exogenous molecules such as antibodies and other soluble factors can also be examined; and (2) Equilibrium and competition binding can be conducted in similar fashion to radiolabeled ligand analysis by simply substituting a fluorescent spectrophotometer for a scintillation counter and measuring cell associated fluorescence instead of radioactivity. Patent Citations
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5 future TLC shows
POSTED: Monday, February 21, 2011 - 10:48am
UPDATED: Monday, March 14, 2011 - 8:21pm
TLC Set to Premiere Five Compelling New Specials
In the coming weeks, TLC unveils five new specials about everyday people living extraordinary lives. From fascinating professions to eye-opening medical conditions, each reveals stories that exist when the remarkable meets the relatable. Previews, photography, and interview opportunities are available upon request. Please let us know if you have any questions.
BABIES BEHIND BARS tells the dramatic story of pregnant inmates contending for a spot in the coveted "baby dorm," a radical new project that allows selected offenders at Indiana Women's Prison, to keep their babies with them while they serve their sentence.
HEAVILY EVER AFTER follows the everyday struggles of Freddie and Kay, an oversized couple making their way in a regular size world. After nearly dying from complications of his obesity, Freddie and his wife Kay got serious about regaining their health. While they battle their weight, they never stop having fun and enjoying the simple pleasures of life. Freddie keeps the house filled with laughter and song, while the practical and tireless Kay keeps them on track as his caretaker and biggest fan.
TINIEST TWEEN: KENADIE'S STORY focuses on the next amazing chapter in 7-year old Kenadie's extraordinary life as a primordial dwarf. Although her entire life has been fraught with enormous challenges, this stage is her most heart-wrenching yet. At just 7 years old, 33 inches tall and weighing 17 pounds, Kenadie is confronted with the possibility of the early onset of puberty, and must face her fear of scary Halloween costumes when her school prepares for the holiday.
From the exotic to the downright bizarre TAXIDERMY USA offers a captivating peek inside the world of taxidermy in America. The special profiles three unique families as they stuff and mount everything from an African Lion, to an American Alligator to a Canadian Black Bear and even a Domestic House Cat. The special follows every step of the journey, capturing the painstaking hard work and the client's personal stories that go along with this mysterious and artistic craft.
From sewage spills, to rodent infestations, crime scenes, and crack houses, the Douglas family are the ULTIMATE CLEANERS, taking on the cases that no one else can � or will. This dirty family business takes former-cop Larry, his brother Matt, Matt's son Josh, Aunt Becky, and friend Patrick on a germ-infested journey that requires breathing masks and hazmats suits. The team tackles the most hazardous homes on the brink of bulldozing, and magically brings them back to life. Dramatic and heartwarming, the Douglas' never give up on a family's home no matter what kind of mess they're walking into.
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There is a program that we have in Pasco County, Florida called BIRTH BEHIND BARS. We teach the pregnant inmates CB classes and we are a doula for them when they are taken to the hospital to have their baby.
We stay for her entire labor and many hours postpartum. If we were not there, she would be alone with only the Deputy in her room.
We are working to see this type of program to be in jails around the country.


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Judge David Hoort's Notebook
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Sanctions for signature by an attorney who is not an attorney of record.
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Thorne v Thorne
, Unpub Per Curiam Opinion (#308382, 11/15/2012) the Court of Appeals affirmed the trial court�s order awarding attorney fees to the plaintiff as a sanction for a non attorney of record signing a subpoena for the defendant's attorney of record without including language �for� or �with permission� in violation of MCR 2.114.<>>
MCR 2.114 requires that every document of a party represented by an attorney shall be signed by at least one attorney of record. A party who is not represented by an attorney must sign the document. If a document is signed in violation of this rule, the court, on the motion of a party or on its own initiative, shall impose upon the person who signed it, a represented party, or both, an appropriate sanction, which may include an order to pay to the other party or parties the amount of the reasonable expenses incurred because of the filing of the document, including reasonable attorney fees. The court may not assess punitive damages. In addition to sanctions under this rule, a party pleading a frivolous claim or defense is subject to costs as provided in MCR 2.625(A)(2). The court may not assess punitive damages.<>>
Under the circumstances of this case, the Court of Appeals could not say that the trial court clearly erred when it found that the one attorney�s act of signing her own name and not indicating that her signature was made �for� or �with permission of� the attorney of record was more than a mere clerical error. �Clerical error� can be defined as, �An error resulting from a minor mistake or inadvertence, esp. in writing or copying something on the record, and not from judicial reasoning or determination.�
In that MCR 2.114, by its plain language requires the pleading to be signed by the attorney of record, an act of certifying the document by an attorney who was not an attorney of record is not a clerical error because the non attorney of record clearly did not inadvertently or mistakenly sign the subpoena.
MCR 2.116(3)(a) provides that a pleading, appearance, motion, or other paper filed by a law firm on behalf of a client is deemed the appearance of the individual attorney first filing a paper in the action.
Subsection (b) provides that the appearance of an attorney is deemed to be the appearance of every member of the law firm.
Any attorney in the firm may be required by the court to conduct a court ordered conference or trial.
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Cell Studios, Studio 1, 21-27 Millers Terrace, London, E8 2DP
Jess Bonham is a London-based still-life photographer, working primarily in the area of editorial and commercial luxury product photography. After graduating from the University of Brighton with a degree in Illustration, she now focuses on creating images based around a love for pattern and precision, reinvention, curious correlations and seeking order out of chaos. Her work touches upon the world of sculpture and installation, often with an injection of humour and surprise.
Previous clients include Chambord, Belvedere, Kenzo, Jaguar, Aston Martin, Selfridges, Ford, AnOther, UK Vogue, Miss Vogue, FRAME, Twin, PORT, Wired, Wonderland, Garage, Viewpoint, The Gourmand and Viction:ary.


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Some of the most closely watched ratings in the industry come out of J.D. Power and associates, which conducts several influential surveys of vehicle owners. Probably the best-known is the Initial Quality Survey, which measures owner satisfaction in the first 90 days of ownership. But a more telling measurement of longer-term quality and owner satisfaction is the company's Vehicle Dependability Study, which measures owner satisfaction of three-year-old vehicles. The overall winning brand in the 2014 study by a substantial margin was Lexus, with only 68 problems out of 100 vehicles reported.
Next closest was Mercedes-Benz with 104 problems per 100 vehicles, followed by Cadillac with 107 PP100, Acura with 109 PP100, and Buick with 112 PP100. The Toyota brand itself was in a three-way tie for sixth place with 114 PP100 with Honda and Lincoln.
Among truck and SUV categories, the strongest performers by company were Honda and General Motors, with Honda taking five out of the 10 categories, and GM taking four. The GMC Sierra took both the light-duty and heavy-duty truck. Honda dominated in the CUV category, with the discontinued Honda Element winning the Sub-Compact CUV category, the Honda CR-V winning the Compact CUV category, the Acura RDX winning the Compact Premium CUV, and the Honda Crosstour winning the Midsize CUV category. Lexus won in the Midsize Premium CUV category with the Lexus RX.
Source: J.D. Power


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Proverbs 22:7 The rich rule over the poor, and the borrower is slave to the lender.
This verse has been stuck in my head ever since Mrs. Stove and I took the Financial Peace course at our church about 5 years ago. Have worked long and hard since to get out of debt (with a few (dozen) gun purchases aside) and finally did it!
Paid off the mortgage today!!
In the words of William Wallace -


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It's hard to take in the extent of the damage done by the flooding in Cork last night. Clonakilty, Douglas, Blackpool, Meadowbrook in Glanmire, Ballyvolane and elsewhere flooded. We were woken around 1am last night by the loudest thunder I ever remember hearing. The storm passed right over Blarney but I think the town escaped the worst of it.
Check #corkfloods on Twitter for further updates.
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