text: 1000 pts Private Investigator Series Quiz <LITEHOUSE CLUB Test Your knowledge of some of the greatest private investigator TV series and earn some points. Sign in to Participate Category: Q & A Tags: quiz, TV SHows Ends: Friday April 19 at 11:12PM EDT Share on Facebook Share on Twitter More Leave a comment Send to a friend! Print this page Pin it! Reddit This! Share on Tumblr! More Ways To Get and Use Points Win a Trip to See Meghan Trainor in Los AngelesWin a Valentine's Day Prize PackWin a $100 Visa Gift CardWin a Winter Emergency Car KitWin $500 in Ski and Snowboard EquipmentWin $200 to Bass Pro Shops


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We stayed at Cabanas Christophe almost a year ago and never managed to write a review however...this place is SO GOOD we simply had to write one. Starting with Christophe, who is the most amazing and friendly host, and continuing on with the place itself - surrounded by a wonderful lush garden. Would recommend this a a place to stay... More
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Despegar.com and Odigeo so you can book your Cabanas Christophe reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Cabanas Christophe Easter Island/Hanga Roa

subset: commoncrawl


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Astigmatism and Your Eyes Center - Lancaster, CAWebMD Physician DirectoryLancaster, CaliforniaAstigmatism and Your EyesWhat Is Astigmatism?Astigmatism is a common eye condition that's easily corrected by eyeglasses, contact lenses or surgery. Astigmatism is characterized by an irregular curvature of the cornea. This is one type of refracti... Other Related Astigmatism and Your Eyes ArticlesEmergency Contact for Lancaster
Nearby Lancaster Hospitals *Antelope Valley Hospital Palmdale Regional Medical Ctr Olive View Valley Care Henry Mayo Newhall Mem Hosp Providence Holy Cross Med Ctr Verdugo Hills Hospital Tehachapi Hospital Pacifica Hospital Of The Valley *Provider Directory Terms of Use: The WebMD 'Provider Directory' is provided by WebMD for use by the general public as a quick reference of information about Providers. The Provider Directory is not intended as a tool for verifying the credentials, qualifications, or abilities of any Provider contained therein. Inclusion in the Provider Directory does not imply recommendation or endorsement nor does omission in the Provider Directory imply WebMD disapproval. |


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SAN DIEGO – The Milwaukee Brewers came to Petco Park last weekend, muscles swelling their sleeves, and played four games.
They left with two runs, both on solo homers.
They also did a lot of glaring at the right-center fence, the one in front of which many of their aspiring homers fell.
"This place can have an effect on teams like that," said David Eckstein, ex-Angel of 2002, now the San Diego Padres second baseman. "That's what we have going for us. Pitching and defense, especially in this ballpark."
That wasn't supposed to be enough. The Padres are 29th among baseball's 30 teams in payroll. Jake Peavy and Trevor Hoffman are just memories, Chris Young is hurt, and Adrian Gonzalez is widely expected to be lockering in Fenway Park later this year.
So, naturally, the Padres came into Monday with 16 victories in their first 25 games. They had the lead in the National League West. And, for the first time, they had a keen organizational sense of where they are and how to behave there.
"They finally did it," said Tony Gwynn, the Hall of Famer, San Diego State coach and Padres broadcaster — not to be confused with his son, Tony Gwynn, the center fielder who is a Petco Park ballplayer.
Gwynn does not get frustrated with the wide-open spaces because that's where he wants to hit. He has 11 singles, 11 walks and five steals and he runs down the dying drives of the frustrated opponents, and General Manager Kevin Towers, since deposed, got him from Milwaukee last year for 30-year-old Jody Gerut.
"This team is athletic now," Gwynn Sr. said. "They're running around making plays, going from first and third. And they can really pitch."
"It's only been a month," said Eckstein, 35, who has struck out twice in 85 at-bats. "I like the fact that our young guys aren't walking around like they've done something special. There's a long way to go but I think we can compete."
This started late last season. The Padres were 41-62 when they traded Peavy to the White Sox for four prospects on July 31. They were 34-25 thereafter, and five times they won series against the contending Dodgers, Giants and Rockies.
"I don't know if the Jake thing was hanging over our heads," closer Heath Bell said, "but the organization did a good job picking up young prospects. What happened was that we just went out there and started playing.
"The young guys weren't trying to make the squad. The older guys weren't trying to put up numbers. And this year we're not worried about beating everybody and making the playoffs. We're just playing ball."
Brian Giles, Tad Iguchi, Jim Edmonds, Khalil Greene, Tony Clark and other stationary members of the 63-99 Padres of 2008 have gone their way. Now there's shortstop Everth Cabrera and slugging left fielder Kyle Blanks, both 23, and third baseman Chase Headley and catcher Nick Hundley, both 26.
Gwynn, Will Venable and Jerry and Scott Hairston grew up as sons of big leaguers, which means they understand the fragility of a hot April.


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Become a healthy eating connoisseur with food & drink recipes for any palate, including vegetarians.
Fri, Jan 30, 2015 8:35 AM
New study finds that a compound in beer may help to fend off Alzheimer's, Parkinson's and other neurodegenerative diseases.
Many of us love sushi, but is our appetite for it furthering the decline of seafood populations?
Farmers are producing less cocoa than the world demands. That doesn't mean we're running out of chocolate.
This sweet and sour soup has a vibrant flavor that will wake you from a wintry hibernation.
The recipe looked great, but the end result was blah. Instead of trashing the soup, maybe it can be made better.
From the Thanksgiving table to restaurants, hiking trips to college dining halls, you can avoid animal products with a little practice.
This wheat product is a good source of protein, and it's gaining popularity as a meat substitute.
Don't let Turkey Day roast your brain. Be organized, keep calm and cook on.
It's been fun to explore the gluten-free world of Portland. Here are some of my favorite finds so far.
Do you think you know what a vegan looks like? If so, this list of folks who eschew animal products might surprise you.
What is archibutyrophobia? Find out this and more as we celebrate National Peanut Butter Lovers Month.
Adding the poblano peppers to this classic soup really kicks up the flavor.
This stew's chile-centric flavor will warm you up from the inside out.
This pot pie recipe can be adjusted to accommodate vegetables during any season.
Keep flatbread on hand and you can whip up a meal while keeping food from going to waste.
Warre's Otima Tawny 10 Year Port will warm you up on a chilly evening.
Creative mom explores how to live a sustainable life with a focus on food.
Mom shares nourishing recipes and blogs about sustainable living.


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John Abbott b. 20 June 1787 Lincoln, MA m. Mary Delano dau of Barzillai Delano. She was born 10 Apr 1789 in Duxbury, MA. They were m 1 Nov 1808 in Sidney, ME. Both died there. Does anyone have any other info on them? I have the marriage record coming from Sidney, but it doesn't tell me much. Any other information would be helpfull. They are my Mayflower connection. Notify Administrator about this message?
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Recent Stories
- Fulbright Scholar to talk motorsports
- Business Partnerships & Enterprise Annual Report 2013-2014 Out Now
- Faculty appoints new dean
- Researchers develop new form of lie-detector test
- REF confirms 'world-leading' research across Faculty
- Envision PhD scholarships: Apply by 15 Jan
- Leverhulme Trust backs infancy research at Lancaster
- Top that!
- University and industry collaborations recognised by prestigious award
- Forty South Lakes Science teachers visit the faculty
£500K Investment For InfoLab21 Resident
CableSense Ltd have secured a £500,000 investment from the UMIP Premier Fund (UPF) to bring to market an innovative intelligent cable infrastructure management system.
CableSense is a North West success story. The idea originated from a business based in Morecambe, and over the last few years the founders have collaborated with InfoLab21 at Lancaster University (where they are now based) and the School of Electronic and Electrical Engineering at the University of Manchester with support from Technology Strategy Board (TSB).
The UMIP Premier Fund (UPF) invests in spin-out businesses emerging from The University of Manchester. UMIP undertakes the stringent business selection of the investments and the fund is managed by the UK's leading technology investment manager, MTI.
CableSense received a £100,000 Proof of Principle investment earlier this year through the technology transfer and incubation process at UMIP, and has now graduated to a full UPF investment.
John Kelly, the founder and Technical Director of CableSense stated:
"This investment provides a fantastic platform for CableSense to productise its technology and prove its business model. Both MTI and UMIP have worked hard to help us put the corporate structure and commercial foundations behind this business and with our technical approach I'm confident that we can fill a substantial gap in the market."
Investment Manager of MTI Partners, Mark Rahn said:
"CableSense proves two things. Firstly that it is possible to build great start-up companies through all North West corporate and multiple university collaborations and secondly that it is possible to shape up a great product and business model in less than a year. This is simple but clever technology that if positioned properly in the market has great potential."
CableSense's product is readily retro-fittable to existing cabling installations at a low cost.
The systems use CableSense's proprietary technology for network cable monitoring, scanning and mapping which substantially reduces the cost of maintaining and running a network including the cost and complexity of moves, adds and changes, fault identification and diagnosis, inventory management and capital expenditure through the identification and re-use of redundant equipment and capacity.
The company will remain at Lancaster for the foreseeable future and it will form part of the growing cluster of high-tech companies across the North-West region.
Wed 25 November 2009
Associated Links
- CableSense Ltd - InfoLab21 Company Profile
- UMIP - The University of Manchester Intellectual Property Limited
Latest News
Fulbright Scholar to talk motorsports
'Motorsport Engineering: Fabulous or Frivolous?'
Mon 26 January 2015
Business Partnerships & Enterprise Annual Report 2013-2014 Out Now
In this report we provide some case studies of our work with external partners during 2013-2014. Read about R&D opportunities with China, new science and technology start-up companies, research with IBM, Booths and regional Small and Medium Enterprises, seed funding for new products and processes, new facilities for hire, free events and training, new companies on campus, plugging the data science skills gap, the Engineering Design Academy, and much more...
Tue 20 January 2015
Faculty appoints new dean
The Faculty is pleased to announce that Professor Peter M Atkinson has been appointed as Dean of the Faculty of Science and Technology.
Mon 05 January 2015
Researchers develop new form of lie-detector test
Police and intelligence agencies around the world have for almost 100 years relied on lie-detectors to help convict criminals or unearth spies and traitors.
Mon 05 January 2015


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Jun 29, 2012, 06:36 PM | |
Considering the amount of time I fly (average) and the amount of time the radio is on either programming for my own planes or programming to provide help for other (extreme) the stock OEM NiMh batt just wasn't cutting it for me.
Buying the Lipo just made sense for me and my current use of the radio. |
Jun 29, 2012, 07:28 PM | |
A couple of months ago I read this great review of the DX7s... http://www.rcgroups.com/forums/showthread.php?t=1579976
The review mentioned that the DX7s had a feature called LST or Limited Standard Trim. I looked everywhere for information on this in the DX7s manual and couldn't find any mention of it at all. I tried asking on that thread but no one answered. In fact, the only place I found anything about it was in the JR11x. Can anybody comment on what LST is and if the DX7s has it or not? |
Jun 29, 2012, 08:46 PM | ||
Would love to take credit but I can't. But I did get the info you are looking for.
Jun 30, 2012, 08:16 AM | |
United States, WI, Burlington
Joined Jan 2005
68 Posts
TM100 Antenna
I had a crash and the antenna on the TM1000 pulled off. Can anyone tell me how to pop open the TM1000 to either replace the antenna wire or solder it back together? It ripped off very close to the "stem".
Also, would this be an acceptable replacement? http://www.hobbyking.com/hobbyking/s...dProduct=16666 Would it have to be soldered in? Thanks to all the pro's on this board. Also: This may be a dumb question, but if I presently do not care to use any info for temp, speed, etc. that the TM1000 I guess is used for, do I need that antenna for anything else other than me just "flying around"? Thanks to anyone from a novice (but learning). |
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Personalized Patriotic Photo Tile Keepsake Box
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I'm debuting a big idea soon (very soon) and am currently hard at work on it. Also, we're currently tinkering with the feed a bit, mainly trying to get the top feature image here to show up there. While I work on all that, I wanted to share with you something else I've been working on – finding my style on Pinterest.
Inspired by all the great remixable wardrobe info. Audrey shared here and here, I'm finally starting to pull myself together. So far I've accumulated some colored pants, a few pendant necklaces, and even two new scarves – I'm telling you, this is no joke. To help in my ever-evolving fashion journey I've recently started four new Pinterest boards and I thought I'd share them today.
Without futher ado, here they are…
Hair Happiness
Braids, braids everywhere! As a mom on the go I'm all about super quick ways to up my do's style factor.
Not Diaper Bags
Along with doing more than a ponytail everyday, another thing I need to change is my bag. With a 3 year old and not-yet potty trained 2 year old, I still need a tote with lots of pocket power but I'm just so over the traditional diaper bag.
Fall Fashion
This is the board with the actual clothes! And accessories… and, OK, right now it's kind of being overtaken by potential Christmas party frocks.
Beyond Friendship Bracelets
Because not all fabulous accessories have to be bought.
As I said, these boards are just beginning – come follow along now to watch them grow.
P.S. Looking for more ways to simplify and save time so you can connect with your family this year?
Check out the sale we're having on our "Kids' Responsibility & Money Management Kit" and "Project Organize Your ENTIRE Life's Planner Printables" - over 45 printable pages in all!
Register for our Free Parenting Webinar this week:
And sign up for our newsletter!


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Illinois Public Media
All articles tagged Bankruptcy
American Dream to Nightmare
Ship of Fools: How Stupidity and Corruption Sank the Celtic Tiger
Page 1 of 1 pages
WILL Highlights
Did you miss this week's 'Downton Abbey'?
Catch it at 9 pm Thursday on WILL-TV. We're doing repeats each Thursday.
Introducing NPR's new show about human behavior
Invisibilia: 10 am Fridays on WILL-AM
PBS is looking for America's most innovative educators!
If you are creatively integrating digital media in your classroom, apply to the 2015 PBS LearningMedia Digital Innovators Program.


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Bennion Kearny Home | Authors | Selected Books | Featured Book | Coming Soon | Competition | Contact Bennion Kearny | Search this Site
About The AuthorDr Mark Duffett is a Senior Lecturer in Media and Cultural Studies at the University of Chester. His research interests centre on celebrity images and fan phenomena. Since first writing Bryan Adams: A Fretted Biography, Mark has published many articles and book chapters on popular music. He is currently working on a textbook about media fandom for Continuum. Bryan Adams Website Links |
Bryan Adams Book ContentsIntroduction Chapter 2 - The First Three Albums Chapter 3 - Reckless Chapter 4 - The Boy Next Door Chapter 5 - Into The Fire: Social Causes Chapter 6 - Waking Up The Neighbours Chapter 7 - Conclusion Chapter 8 - Bibliography Afterword |
About this BookThis book is a lightly revised edition of - Praise for the 1994 edition: "Overviews of other Canadian bands and artists... are included in this
interesting book. Bryan Adams' successes throughout the 1980s and 1990s are
well documented... Duffett makes a point of praising Adams' songwriting
abilities... Overall, Duffett's biography of the 'Groover from Vancouver'
Bryan Adams is well written and a fine introduction to Adams' career for
most fans". "The book impresses with facts and figures. It also explores Adams'
'regular guy' image". "Bryan Adams, A Fretted Biography is something that you don't see very
often... It also contains Canadian press information on the man that was
previously unavailable in the UK". |


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Fingerlab has introduced DFX – a real-time Quad Multi-Effects Processor for iPad.
Here's what they have to say about it:
Plug in any audio source, a guitar, a bass, a microphone, a DJ mixer. Or play your favorite iOS synth or drum machine with Audiobus or Apple Inter-App Audio. Or why not playing a track from your iTunes Music Library. Select one, two, three or four effects amongst 35 hi-quality digital audio effects and explore endless sonic possibilities. Delays, echos, reverbs, filters, tremolo, autowah, compressors, distortions, vocoders, resonators, etc… more possibilities that you can imagine.
At last, but not least, DFX is also a great little sound design machine. Just select one of the built-in tone synthesizers on the first effect and let your imagination build new sound landscapes at the tip of your fingers. We have had a lot fun making ours in the Factory presets.
No in-apps, no ads, no fuss. DFX is simple, intuitive and fun. No user guide required ;^)
Here's a demo video for DFX: Continue reading


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RPG » M. P. S. I. Into The A. L.G.R.Y. W.C.
"That's the problem!" exclaimed Rain. "I can't go home!"
Sakir looked a bit confused. Rain sits down next to him, then she begins her ramble.
"Remeber how i crashed my ship back at that forest...well, as long as i cant fix it, i'm stuck here!" She then looks at him and grins from ear to ear, "That's why i came to find you! so you can help me.......now that we're friends and all."


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From Barnes & Noble
The Barnes & Noble ReviewThis beautiful trade paperback volume allows readers to revisit three magical romances, originally published in the 1980s under the pseudonym Kay Robbins. Verbal fencing and sensual teasing mix well with humor and drama in these classic stories. In Kissed by Magic, it's not exactly business as usual when a beautiful corporate president agrees to swap jobs with her handsome executive assistant to clinch a land deal with an eccentric businessman. Of course, it turns out there's more than one hidden agenda…and business becomes pleasure amid some surprisingly magical delights. Trevor King's intentions are noble when he comforts Taylor Shannon in Belonging to Taylor, but he can't help but be bewitched by the lovely psychic's charm, even when she declares that they're meant for each other. In Eye of the Beholder, talented artist Tory Michaels isn't sure what to make of it when a stranger proposes to her at first sight. The beguiling, seductive man seems determined to convince her that he belongs in her life…but first he needs to convince her that he has nothing to hide. Sue Stone


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Serving Thousands of Needy Alexandrians Each YearALIVE! (ALexandrians InVolved Ecumenically) is a 501(c)3 nonprofit organization of volunteers from over 40 religious congregations and the community working together to help those in need in Alexandria, Virginia. Founded in 1969, ALIVE! is the largest private safety net for the needy in the city of Alexandria, addressing short-term to long-term needs for those less fortunate in the community. ALIVE! serves thousands of Alexandrians annually with shelter; low-cost early childhood education and childcare; financial help for rent, utilities, medical care and other critical needs; emergency food; and deliveries of donated furniture and housewares. Learn more in this video about ALIVE! programs: Video by Susan Hale Thomas, a local photo journalist.
Hunger Free AlexandriaIn September, ALIVE! member congregations conducted a Hunger Free Alexandria event at Fairlington Presbyterian Church to foster increased commitment to ending hunger in our city. Visit the Hunger Free website for more information. Alexandria Childhood Obesity Action Network Food Study ReportThe Partnership for a Healthier Alexandria's Childhood Obesity Action Network (A-COAN) has released a new report, "Toward an End to Hunger in Alexandria," commissioned by A-COAN with other Alexandria organizations. The study examines food hardship in the city and obstacles and remedies to providing more assistance to needy residents. The study's authors looked at the emergency food assistance system in Alexandria, availability and use of government food aid programs, and private sector food assistance efforts. To learn more about the report or to get connected with the partnership, visit the Partnership for a Healthier Alexandria's website or email firstname.lastname@example.com. ALIVE! Legacy SocietyLearn more about the ALIVE! Legacy Society, a community of friends and supporters who have provided for ALIVE! in their wills or trusts — or who have formally expressed the intent to do so. Support ALIVE!Put your charitable contributions to work in the local community. Consider a tax-deductible, donation to ALIVE! Use your credit card to securely donate online now. Learn MoreMore than 700 volunteers a year help ALIVE! fulfill the mission to put faith and love into action. Of ALIVE!'s seven programs, five are staffed and managed entirely by volunteers. ALIVE! is Alexandrians helping Alexandrians. Find out more about ALIVE! and our many volunteer opportunities and wish lists of needed items for our programs. Connect with us on facebook, post on our our wall, join in a discussion, and much more. And don't miss our video on . ALIVE's Vision and Mission
* * * Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8 (NIV) |


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I had bottled a hefe using TAP and bottles. TAP is down to half. I am going to a BBQ and was considering bring the TAP, but wanted it full. Any problem cracking open several bottles and CAREFULLY pouring bottles into TAP container, Aside from possible oxidation by introducing O2? I plan on the finishing the TAP container the same day, so i am not overly worried about oxidation. Anything? flat beer? other problems???
Alcohol may be man's worst enemy, but the bible says love your enemy.
Answered my own question. No problems adding bottled beer to top off tap-a-draft. 75-80% was finished leaving a little which i'll probably finish within a few days. Tasted great. kept CO2.


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We had a great time-within walking distance of almost everything in town. The two bedroom cabin was adorable-clean, roomy and parking right at the front door. Cooked breakfast in the fully stocked kitchen, walked the path along the river which was only 1/2 block from the door. Chris and Debbie have a wonderful place and make you feel as though... More
- Also Known As:
- Three Bears Hotel Red River
- 3 Bears Lodge


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The 76 stationery items needed for school
Stumped on what to buy your child for school? Here's what you need. more
8 easy, family friendly recipes each week
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Apricot Chicken is a classic recipe that is a real crowd-pleaser – and when you have a recipe up your sleeve for it that is this easy, there's no reason you can't make Apricot chicken any night of the week!
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Heat 1 tbsp oil in pan, brown chicken, add onion, cook 2 minutes.
Add combined apricot nectar, ½ cup (125mL) water and MAGGI Recipe Base, bring to the boil stirring.
Cover and simmer 30 minutes, or until chicken is cooked, stir occasionally.


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British women Red Cross workers posing in front of a tent. Several close up shots of the women. They smile. Two small boys walking across field. More shots of the women Red Cross workers leaving tent and posing.
We always welcome comments and more information about our films.
All posts are reactively checked. Libellous and abusive comments are forbidden.


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Banks received billions in support during 2008-09 crisis, report says
Canada's big banks received billions in support from the federal government and the Bank of Canada during the 2008-09 financial crisis, a report by the Canadian Centre for Policy Alternatives says.
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OTTAWA—Canada's big banks received billions in support from the federal government and the Bank of Canada during the 2008-09 financial crisis, a report by the Canadian Centre for Policy Alternatives says.
"While these funds were repaid in full, it is clear that the banks benefited enormously from public financing when private funds were unavailable," wrote David Macdonald, author of the report released Monday.
"In addition, had the rapid and enormous deployment of public funds not been available, most, if not all, Canadian banks would have encountered serious difficulty."
The left-leaning think-tank estimated the country's largest financial institutions borrowed nearly $75 billion in short-term collateralized loans from the Bank of Canada and the U.S. Federal Reserve, peaking at $41 billion and $33 billion respectively.
The banks also sold a total of some $69 billion worth of insured mortgage-backed securities to the government, the report estimated.
The Centre for Policy Alternatives based its report on information provided by Canadian public institutions and analysis of the banks' financial reports.
The report noted that much of its work was based on estimates because the federal government and the Bank of Canada have refused requests for a detailed accounting.
"A healthy financial system cannot be based on massive government support for which the details remain secret," the report said.
"It is only through an honest and transparent examination of what occurred and how it can avoided in the future that a stronger financial system can be built, which is in everyone's best interest."
Ottawa has said repeatedly that Canada has one of the soundest banking sectors in the world and the country's banks did not require a bailout during the 2008-09 financial crisis.
However, the banks did receive help from Bank of Canada programs to make borrowing money easier and CMHC's insured mortgage purchase program that helped the banks raise cash.
Finance Department spokesman Chisholm Pothier said the government extended financing to the Canadian banks, not subsidies, to allow them to continue to provide credit to businesses and consumers during the crisis.
"The credit was extended at competitive interest rates to protect taxpayers. Financial institutions accepting this credit paid interest on the loans," Pothier said.
"Not only did these measures play an important role in supporting Canadian business during the credit crunch, they also made money for Canadian taxpayers."
In the U.S., Congress authorized $700 billion (U.S.) for the bailout of financial companies and automakers during the financial crisis under its Troubled Asset Relief Program, or TARP.
A report last week found that the U.S. government is still owed $118.5 billion (U.S.) including $14 billion (U.S.) that has been written off or otherwise lost.
Top News
- NEW Conservative government's terror bill increases spy agency powers
- Study backs up ineffectiveness of this year's flu shot
- Supreme Court strikes down law that prevents public sector strikes
- Home health care workers on strike across Ontario
- Prepare yourself for battle to fight a ticket: Roseman
- Updated Loonie falls yet again on unexpectedly bad GDP news
- Couple now accused of three separate murders in same family
- NEW Fear over Greece-EU showdown is overdone: Olive
Special Coverage
China turns to spuds to sate its growing appetite
Beijing itself has launched the revolution, in the name of 'food security.' Potatoes use far less land and water than rice, wheat, or corn. But the tuber's roots don't run deep in the cuisine of the world's most populous nation.


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The SalaryMan
An indirect Sequel to "The Artist" shows the life of the salaryman
4.45 / 5.00 6,177 ViewsCornelius & Toots 4
Cornelius & Toots are back, and a Haunted House Ride seems a little spookier than usual!
4.49 / 5.00 2,034 ViewsStreet Fighter Sprites can be found here: http://explode.to/midnig
Update: Alot of you were saying that the animation was too slow so I've increased the frame and it actually does look better :D I will use this frame rate in Part 3 as well! Enjoy Peeps!
You all wanted Part 2 so here it is...Part 3 is in the making and should be along soon, its going to be the BIG one...RYU vs KEN! Until then enjoy Part 2 Peeps!
where did u get the sprites?
awesome anim man!!!
not bad
its slow but smooth, however i couldnt do any better myself, good job man


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FoCS Corporate Connection - Juniper Networks Company Day
Contact Name:
Jenna Whitney
Contact Email:
GDC Atrium
Oct 2, 2013 2:00pm - 5:00pm
Stop by and learn more about Juniper Networks!
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2408 Martin Luther King Drive
Little Rock, Arkansas (AR)
Pulaski County
(501) 372-4129
For your convenience, flowers can be ordered online and delivered by a Local FTD Florist for an upcoming Service and/or delivered to:
Hardy Funeral Home
2408 Martin Luther King Drive
Little Rock, Arkansas
Questions such as "Which of the rituals should I attend?", "When should I visit?", "What should I say, or not say", "Do I make a phone call?",
"How do I send flowers to Hardy Funeral Home?" are common questions for most Family and Friends during this time.
Understanding terms such as "Funeral Service",
"Private Service" or "Memorial Service" can be confusing as well."Who creates the Eulogy?", "What is an appropriate dress code?", "Can I, or should I bring my Children?"
are common questions as well.
Contact Hardy in Little Rock, Arkansas to help answer these questions. Online funeral resources can be helpful as well.


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As scientists call for increased investments in aquaculture to reduce fishing pressures on Lake Malawi, one of the world's fresh water bodies and home to over 500 endemic species, a recent study reveals that lack of steady ...
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Charlie Dare's curator insight,
March 11, 2014 11:43 PM
A review of past years highs and lows in Aquaculture ~
John Bostock's curator insight,
December 16, 2013 1:44 PM
A bit of sales talk but provides a good insight into commercial products for the very important function of environmental monitoring and control in aquaculture
John Bostock's curator insight,
November 10, 2013 10:00 AM
A pre-Christmas treat for aqua-techies like me! |


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Meeting Notes - June 01, 2004
Ten Years after Genocide in Rwanda: Building Consensus for the Responsibility to Protect
Angela Ndinga Mvumba, rapporteur
On the morning of March 26, 2004, members of the UN community shared a somber minute of silence to honor the over 800,000 victims of the 1994 Rwandan genocide.
Thus began the Memorial Conference on the Rwandan Genocide, which was jointly organized by the governments of Rwanda and Canada to ensure that the lives lost in Rwanda are remembered and to examine the continuing implications of the Rwanda tragedy for the international community. The conference was organized on the eve of April 7, 2004, which was designated by the UN General Assembly as the International Day of Reflection on the Genocide in Rwanda. Participants noted the importance of drawing attention to a new normative framework the principles of Responsibility to Protect which is outlined in the final report of the International Commission on Intervention and State Sovereignty and addresses the policy dilemma of how the international community should protect the lives of civilians from genocide, war crimes, or gross and systematic violations of humanitarian law.
The Global Observatory
Research Suggests Discrimination Against Muslims in France Likely to Worsen
Recent behavioral research shows there is a basic discriminatory bias against Muslims in France.
Key Global Events to Watch in January
A list of key upcoming meetings and events with implications for global affairs.
2015: Ten Multilateral Events to Watch This Year
A list of ten events that are likely to impact international peace and security in 2015, compiled by IPI's Francesco Mancini.
The Global Observatory, produced by IPI, provides timely analysis on peace and security issues, interviews with leading policymakers, interactive maps, and more.
Recent Events
January 20, 2015
Mongolian Foreign Policy Between ''Two Giants''
On January 20, Mongolia's new Foreign Minister Purevsuren Lundeg visited the IPI Vienna office and gave an informal briefing on Mongolia's contemporary foreign policy priorities and challenges.
January 20, 2015
Dutch FM Koenders: ''The Security Council Has to Change''
Speaking to an overflow IPI audience on January 20th, Dutch Foreign Minister Bert Koenders acknowledged how far the United Nations has come since its inception 70 years ago but said that the organization still "has a lot of growing up to do."
December 15, 2014
Fathi: Iran and the Struggle Between Hardliners and Reformers
Discussing her new book The Lonely War: One Woman's Account of the Struggle for Modern Iran at IPI on December 15th, author Nazila Fathi said that 35 years after the revolution, Iran is divided between hardliners and a large moderate middle class, but admitted that it is still unclear which of the two sides will gain the upper hand.
Latest News
September 25, 2014
IPI Remembers Margaret Vogt
August 25, 2014
New IPI Documentary Film on Past and Future of International Affairs
July 31, 2014
IPI's Hoge Al Jazeera Interview on Gaza, the UN and Press Coverage


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Sean C & LV - Where's Your Leader?
Artist: Sean C & LV
Featuring: Prodigy, Bun B, CharlieRED
Producer: Sean C & LV
Album: Loud Dreams Vol. 1
Avg Rating: 3.3 ( 3 votes )
With the myriad troubles facing the world today, we could use a Martin Luther King, Jr. or a Nelson Mandela to take charge and steer us toward a brighter tomorrow. On the lead single off their forthcoming full-length, veteran producers Sean C and LV pose a rhetorical question to all those who say they want change: "Where's Your Leader?" A few prominent rhymesayers answer their call; Mobb Deep veteran Prodigy, UGK alumnus Bun B and World's Fair member Remy Banks each jump on the headliner's street-ready instrumental to spit hard-hitting, contemplative guest 16s. CharlieRED frontman Rocki Evans (who will now go by his birth name, Chauncy Sherod) rounds out the lineup, imbuing the chorus with his ragged brand of soul. After listening to this jam, are you ready to follow wherever Sean C & LV may lead? Then watch our pages leading up to their Loud Dreams Vol. 1 LP, presented by Play Cloths and scheduled to drop March 25.
Sean C & LVProdigyBun BCharlieRED DownloadsHitmaker
Written by richard on 02/20/14
Submit your Rating
Grind Music
First DJ Booth Appearance:
"Live For Today" (2014)
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text: The Oklahoma Sooners are No. 1At least according to one college football preseason ranking.Phil Steele's College Football Preview, one of the major annual preseason college football magazines, ranks OU No. 1.That's right, No. 1.The rest of Phil Steele's Top 25:1. Oklahoma2. Ohio State3. Alabama4. TCU5. Nebraska6. Boise State7. Florida8. USC9. Miami10. Oregon11. Texas12. UNC13. Va. [...] To Read more go to OU Sports


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Stephanie Marley is one of about 185 new teachers hired in the Higley Unified School District this school year.
The sixth-grade math teacher at San Tan Elementary School in Gilbert said she chose the district because she lives in the community and heard good things about the district.
"I love my school. I love the kids, the administration, the community," said Marley, 43, who always wanted to be a teacher and is in her third career. "I really couldn't see any reason why I would choose to leave."
Marley said she plans on retiring from the district, and hopes to stay at San Tan Elementary.
That's exactly what the Higley district wants to hear.
As Higley, along with other school districts. struggles to find ways to retain its staff, the district is checking back with former employees to shed light on ways to improve staff retention.
The district will begin sending out surveys in the next couple of weeks to teachers who left last year to determine why they left and what the district could have done, if anything, to keep them, said Denise Birdwell, assistant superintendent for human resources.
"We know through research that the reasons people stay has a lot to do with the environment and the support they receive," said Birdwell, in her first year with the district and her 26th in education. "We want to do everything we can to be supportive and moving them toward their professional goals."
The district saw 102 teachers leave during the last school year, seven of them retirees, out of the district's 377 teachers. That means it is retaining about 75 percent of its teachers, a percentage they would like to see increase, Birdwell said.
Among questions for which the district seeks answers:
• Are teachers taking jobs at another district, leaving to relocate, or just leaving the educational field?
• Did teachers feel supported in their job, and did they have the necessary teacher materials to instruct their students?
• If they had the opportunity to come back to the district, would they?
The district hopes teachers will start responding in the next month, Birdwell said.
"We will look at their reasons for leaving and will create an analysis," she said. "We will report the data back to the (governing) board, and this will be a data point that we will use in our recruitment process."
If a number of teachers who the district hired leave to move back to their home states, the district may consider scaling down its out-of-state recruitment, Birdwell said.
"Retention and recruitment go hand in hand," she said. "You've got to approach what's happening on the retention level the same time you're recruiting."
If teachers are leaving to pursue higher degrees, the district might consider offering a master's or doctoral program that teachers can take after school so they're not leaving to continue their education, Birdwell said.
"They may be more likely to stay employed, and for the district, we're able to retain that teacher," Birdwell said. "It will be interesting to see if this is what's happening. I'm a firm believer that you collect as much data as you can."
The district also wants to look internally and will survey all staff members in the spring to determine their levels of job satisfaction.
The district will also gather data on the success of its teacher mentor program to judge if it's working to help new teachers in their first three years.
The Gilbert district uses information from letters of resignations, exit interviews that principals conduct and data gleaned from aztwc.org, the Arizona Teacher Working Conditions Survey, in its retention efforts.
Also, one of the reasons the district recently filed a lawsuit against the state regarding the Career Ladder Program was teacher retention, said Nikki Blanchard, Gilbert's assistant superintendent of human resources
That program is a performance-based compensation plan currently providing incentives to teachers in 28 districts around the state who choose to make career advancements without leaving the classroom or the profession.
"We felt it was important that our teachers have the same opportunity for Career Ladder as other districts," she said.
In the suit, the district argues it is at "a disadvantage in attracting and retaining good and experienced teachers" because it is excluded from the program.


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First Reform Church Holds Marriage Rally on Father's Day After Sunday Services
June 19, 2011 by David Contreras Turley, Associate Regional Field Director
On Sunday, members of the faith community all across the Empire State gathered to show their strong support for full marriage rights for all New Yorkers. Parishioners throughout the state, including Rochester, Buffalo, the Capital Region, Long Island, and Staten Island, came together this Father's Day after Sunday services in solidarity for marriage for all loving New York couples.
In Hudson, Pastor James Eastland of the First Reformed Church invited parishioners and citizens to write letters and make phone calls to our New York Senators to urge them that the time to pass marriage is now and show that there is strong support for the marriage bill amongst the faith community. Various supporters were on hand, such as gay best friends Charlie Ferrusi and Timothy Howard, who made global news in 2010 when they were crowned Hudson High Prom King and Queen. Also in attendance were Linda Mussmann and Claudia Bruce, the first couple to be married by the mayor of Hudson once the marriage bill passes.
The faith rally took place during Hudson Family Pride Day, a free street festival in celebration of LGBT families and allies of Columbia County and beyond.
Join us and help make history in New York this week. To get involved, email David Turley at firstname.lastname@example.com.
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January 23, 2015
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January 24, 2015


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The website specifically mentions that most women's eyebrows are too thin and that eyebrows are very important. I was very nervous about my brows being made too thin and said so repeatedly. I specifically and emphatically asked that my rounded heavy brows be very conservatively cleaned up and now they're pointed on top and thin! It has been several months so I think she permanently ruined my brows and the whole l... view more
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Today:8:00 am - 4:00 pm
49 E Oak St,
Chicago, IL 60611
(312) 587-8727
Business Details
- Hours:
Regular Hours
- Payment method:
- debit, discover, master card, visa, cash, check
- Neighborhoods:
- Streeterville, Central Chicago, Near North Side
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- English, French
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- Beauty Salons, Skin Care, Cosmetologists, Day Spas, Hair Removal, Make-Up Artists, Hair Braiding, Cosmetics & Perfumes, Hair Stylists
girl6061002/01/2007Ruined my eyebrows! This is only a review of one service but it was a doozy...


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Bluetooth GPS Mouse - free
by Argotronic
Use an external Bluetooth GPS receiver as GPS source in your applications!
YOU HAVE TO ENABLE Settings -> Applications …
10,000+ downloads, Rating 4.03 (202)
by Everstov Vasiliy
Сахалыы таабырыннар.
Якутские загадки.
Приложение "Таабырыннар" содержит более 100 загадок на якутском языке.
С помощь …
1,000+ downloads, Rating 4.44 (163)
John NES Lite - NES Emulator
by John emulators
John NES Lite is NES/Famicom emulator for android 2.3+.
This app does not work without your own game files.
- …
1,000,000+ downloads, Rating 4.07 (24704)
Best Vibrating Metronome
by Netigen
Do you need a reliable device that accurately presents the tempo for each played song?
Do you want to have full control …
10,000+ downloads, Rating 3.81 (189)
Go Musics
by Willsin Hajo
Listen and enjoy your favourite music on Go Music.
Recent changes:
-New icon
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MemoryBlock Pro
by Category 5 Games, LLC
The TOP app from iOS debuts on your Android!
No Ads!
Inspired by the classic Simon color memory game.
How good is you …
100+ downloads, Rating 2.67 (3)
Top Hat Soaring (beta)
by Robert Dunning
Top Hat is a project to create an intuitive glide computer for soaring.
"Everything should be made as simple as possibl …
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Personal.com Data Vault
by Personal, Inc.
"A life management platform" – The Economist
Master life's details with Personal's award-winning, cloud-based Data Vau …
50,000+ downloads, Rating 4.15 (371)
Funny Camera Toy
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Take funny pictures and share them with Camera Toy !!!
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Quantum Spectre Study Version
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by Føroya Tele
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(English in next section)
Við hesum forriti kanst tú leita eftir fólkum
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Burgernet NL
by WorldAppz
Gebruikers van deze app ontvangen burgernetberichten van de politie in de omgeving van de locatie waar zij zich bevinden …
100,000+ downloads, Rating 4.11 (4757)


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Ga. Southern 12
(3-3, 1-3 away)
North Carolina 42
(4-2, 3-1 home)
3:30 PM ET, October 10, 2009
Kenan Stadium, Chapel Hill, NC
Top Performers
Passing: T. Yates (UNC) - 118 YDS
Rushing: R. Houston (UNC) - 15 CAR, 56 YDS, 3 TD
Receiving: S. Draughn (UNC) - 5 REC, 43 YDS
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Black Inventors
in Education
This week Kai talks with Keith C. Holmes author of Black Inventors: Crafting Over 200 Years of Success. Kai met Mr. Holmes at The 43rd Annual Legislative Conference of the Congressional Black Caucus this year and invited him on the show he graciously accepted. He is a fan of Keith's work and can't wait to discuss with him the history of Black innovation and ingenuity that he has researched and unearthed all over the planet.
Black Inventors by Author Keith Holmes / Mr. Chip Coffey on Mental Health
in Culture
Black Inventors, Crafting Over 200 Years of Success, highlights the work of Black inventors from over seventy countries. The author, Keith C. Holmes, has spent more than twenty years researching Black inventors around the world. Without inventions, innovations, financial resources, materials, muscle and labor saving devices, civilizations cannot exist and flourish. Author Keith Holmes will dispel myth and outright falsehood about the contribution that the Black race has made in inventions around the world.
My first guest for this week show will be Mr. Chip Coffey. Mr. Coffey is The Director of Theraphy Services for St luke's Behavioral Health in Phoenix, Arizona. I will be speaking with Mr. Coffey about the impact of mental illness on our society. Please listen into the show and participate
Show time: 1pm Arizona time, For my BlogTalkRadio audience the number is 424-222-5349 If you would like to call "The Charlie Bolts Show" you will need call this number (408) 418-5050 once you give this pin number 81810# you can join the live show. Any electronic device that has Internet capability will allow you to join "The Charlie Bolts Internet Talk Show". Our web links are www.thecharlieboltsshow.com and www.winsongentertainment.com if you go to either of these web links you should be able to participate in our live interview.
Being social for inventors is important!
in Business
Being social for inventors is important!
Find out why social networking is important and also what not to do on social networks. Learn more by visiting us at www.inventright.com
Female Black Inventors Rock!
in Lifestyle
Yvette Kendall loves to invent things and has many patents pending. Founder of FBI Rocks! a program to honor the works of female black Inventors, Kendall looks to generate awareness for these unknown women.
African American Inventors
in Lifestyle
In honor of Black History Month, join me as I pay tribute to black inventors. Products that we use every day, were invited by some brilliant people of color.
Black Businesses, Black Inventors, Black Education
in News
TRS Android App
TRS 3 point plan
Talk Real Solutions Radio Page
The views expressed on talk real solution hosted by Tyrone Thompson at blog talk radio are my own and do not reflect those of Blog talk radio, Youtube and etc . The content here belongs to the Talk real solutions and it's many contributors. Views and opinions expressed by all contributors belong to them and not Talk real solutions or Tyrone Thompson, the blog owner, host and etc .
All data and information provided on this site is for informational purposes only. Talk real solutions makes no representations as to accuracy, completeness, correctness, suitability, or validity of any information on this site and will not be liable for any errors, omissions, or delays in this information or any losses, injuries, or damages arising from its display or use. All information is provided on an as-is basis
Entrepreneurship Week - Women Inventors FYI
in Women
Did you know that 1 in 11 American women are entrepreneurs? The Women Inventorz Network helps women realize their entrepreneurial dreams- we are excited to feature one of the founders and an inventors expert, Dhana Cohen as well as her colleague Melinda Knight in honor of Entrepreneurship Week February 2013.
MyCity4HerRadio host and Executive Producer Monyka Berrocosa will talk about all the things women who are investors, or thinking of commercialising a product should know and be aware of. MyCity4HerRadio is produced by MyCity4Her Media - which also publishes MyCity4Her.com.
Women Inventorz Network (W.I.N) is the only independent organization for women inventors in the US and Canada. Founders and inventor experts, Dhana Cohen and Melinda Knight help women inventors by evaluating products based on 10 points of retail readiness, marketing, logo design, website effectiveness, etc and provide guidance in making necessary improvements. W.I.N. is a connector and launch pad for women inventors to get their products found.
For more information about W.I.N. visit their web site at:
Black Inventors, Innovators, Pioneers, Luminaries
in Culture
Please join us as we discuss Black inventors, innovators, developers, pioneers, and luminaries.
The call-in number is 310-982-4273. You can also Skype into the show.
Time: 10AM PST/Noon CST/1PM EST.
Black Farmers United with President, Gary R. Grant
in Education
Inventor and Farmer, Andrew Beard~Born in Alabama in 1849, Andrew Beard spent the first fifteen years enslaved on a small farm in Alabama. A year after he was emancipated, he got married and became a farmer in a small city outside of Birmingham. While in Birmingham, he was able to develop and champion his first invention (a plow). Three years later, he patented a second plow. These two inventions earned him almost $10,000, with which he began to invest in real estate.
Tune in tonight to The Gist of Freedom as we discuss the history of Black Farmers with Gary R. Grant. Mr. Grant is the president of The Black Farmers and Agriculturalists Association.
Read Mr. Grant's paper "Black Farmers United: The
Struggle Against Power and Principalities"
The authors are the President of the Black Farmers
and Agriculturalists Association, Tillery, NC,
Assistant Professor of Sociology at Kansas State
University, and doctoral candidate in Sociology at
Kansas State University, respectively.
Please address any correspondence to email@example.com.
Following his stint in real-estate, Andrew Beard began to work with and study engines. In 1892, he filed a patent for an improvement to the rotary steam engine.
Mr. Beard was inducted into the National Inventors Hall of Fame in Akron, Ohio for his work on railroad coupler design.Andrew Beard invented the JENNY coupler for railroad cars.
Little is known about the period of time from Beard's last patent application in 1897 up until his death. He died in 1921.
Pets Teach Us So Much Radio 151 with Robbin and Joseph Everett
in Pets
Always fun and informative, this dynamic couple from the #1 rated web tv, blog, radio and podcast show for animal lovers, Pets Teach Us So Much, Robbin and Joseph Everett entertain millions of people from all over the world with segments about:
-Pet health and nutrition
-Pet behavior
-Learning from our pets-how to have better relationships with the people in our lives with The Love Genies segment
-Animal stories from around the world
-Guests include, authors, veterinarians, bloggers, celebrities, product inventors, charity spokespersons and animal behavior experts.
For more information about the global leader in pet related new media entertainment, Pets Teach Us So Much Radio, Podcast, Blog, web tv, Robbin and Joseph Everett, or to book an appearance, go to http://www.TPPC.tv.
Sonia Barrett Speaks 2 Perceptual Need 4 Validation & Approval, co-host GodIs L
in Spirituality
Please join us to welcome Sonia Barrett to "Get Your, It Together!" REVELATION aka, introduces his NEW co-host "GodIs L," as they interview this world renowned and internationally acclaimed author, now turned director and executive producer of her latest documentary film, "The Business of Disease." This is Sonia's directorial debut, as well as executive produced, writer and performer of the narration in the new film. This recently premiered documentary has the scientist, doctors, researchers, naturopaths and inventors all contributing a chapter in one of her recent books, entitled "Health: An Inside Job, An Outside Business." She also host and is the producer of the Inner Bridge Radio and is the publisher of Reality Bridge Magazine. Additionally, she is the founder of Timeline Publishing & Media Relations and has hosted a plethora of workshops, retreats and has held a myriad of forums and lectures. You can visit her website www.spiritinform.com, or call 818-899-1133. Sonia is considered to be one of the most profound mystics of our time and an expert in the realm of motivating and encouraging the trusting of our instincts which should precede external approval. Sonia will share her wisdom as it relates to the perceptual need for validation and approval, it affects on our personal growth, transformative experience and how it can be a hindrance to raising consciousness and becoming spiritually self. This show will be uniquely empowering and thought provoking, as we affirm that we're all activated seeds of pure consciousness, animated by The Divine Spirit of The ALL There Is!
Keith C. Holmes - Black Inventors Crafting 200 Years Success
in Self Help
Join Paul Lawrence Vann as he hosts author, Keith C. Holmes who shares his incredible book, "Black Inventors Crafting 200 Years of Success.
Keith C. Holmes is goign to provide a behind the scenes look at black inventors and how their inventions make our daily lives better each day.
Mr. Holmes book documents quite a few inventions, patents, and labor saving devices conceived by Black Inventors. This book provides insight on Black inventions from around the world.
Keith C. Holmes introduces inventions, both past and present that balck people invented and patented globally and multiculturally. There is no other book like this one and that's why listeners should tune in to discover more about Black Inventors Crafting 200 Years of Success.
Tune in, listen in and call in with questions at (877) 404-1615 or join us in the web chat room: http://www.blogtalkradio.com/paullawrencevann
- ...


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Over the weekend of 13-16 December 2014 the Economic Freedom Fighters held their first national congress. The event was attended by more than 2000 delegates, representing more than 500 000 members. This was an excellent turnout for a party which is just over one year old!
Boko Haram has carried out a number of suicide bomb attacks, such as the one that took place in Maiduguri in northern Nigeria earlier this week, in which 78 people were killed. Over the last four years, suicide bombings have become one of the avowed weapons of the right-wing Boko Haram insurgency campaign in Nigeria, especially in the northern part of the country. Musa Atiku explains the background to all this.
A revolutionary situation is currently spreading across West Africa. Over the last few days and weeks, big movements of the masses have shaken the regimes of Togo and Gabon to their foundations. It is clear that these are not isolated events. Rather, it is part of a revolutionary wave which has the potential to engulf the entire region.
To properly understand and locate the underlying factors that have led to the fragmentation of Cosatu, one needs to go no further than the South Africa Communist Party.
The expulsion of NUMSA from the COSATU trade union confederation, is one of the most significant events in the African class struggle in the whole of the past period. It clearly exposes the reactionary character of the present COSATU leadership who are willing to tear the union apart rather than allow a militant union to remain within its ranks, even if this means paralysing the workers' movement in order to preserve their own privileged positions.
As a party that purports to stand for the unity of workers, one expected that the South African Communist Party (SACP) would do its utmost to avert the implosion that is tearing the Congress of South African Trade Unions (Cosatu) asunder.
In the early hours of Saturday morning, 8 November, the Central Executive Committee of South Africa's largest labour federation, COSATU, voted by a margin of 33 to 24 to expel its biggest affiliate, NUMSA from the federation. This decision will have far-reaching consequences not only for COSATU, but for the liberation movement and the direction of the class struggle.
Page 1 of 35


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Microsoft starts taking sign ups for its Skype Translator preview
Microsoft is seeking testers for its Skype Translator real-time video translation service, which will be available in limited preview form before year-end.
Microsoft is seeking testers for its Skype Translator real-time video translation service, which will be available in limited preview form before year-end.
Dish has warned of irreparable damage being dealt to the online video industry and the competitive nature of the business if the merger between Comcast and Time Warner Cable goes ahead.
With every month that goes by, Netflix and other real-time video entertainment providers and their customers are dominating Internet usage more and more.
Online YouTube video network FullScreen is reportedly of interest to Time Warner as the company seeks ever-increasing audience scope and sizes for its advertizers.
Facebook has been baking in video support throughout its channels for some time now, so the time is ripe for monetizing this particular piece of digital content.
According to Sandvine, real-time video and audio entertainment traffic takes up most of the Internet's bandwidth.
Cisco and Mozilla reps declared that the free, open distribution of the codec enables streaming of real-time online video from the browser without plugins.
A free iOS app turns any iDevice into a free, wireless security camera that records and streams video via the cloud and allows you watch it remotely in real-time.
A workplace collaboration platform might not be worth anything without real-time functionality these days, so Citrix has stepped up its game.
Forget video conferencing, big problems demand face-to-face time. On a plane. In the air.
A new video talk show has started broadcasting today to explore the role of marketing and IT in the enterprise. Come watch at 4:00pm Eastern Time.
Now not only will you know how many people clicked on your YouTube video, but you can find out how engaged they were based on time watched as well.
Curiosity's descent onto Mars and follow-up imagery are being hosted in the cloud.
NASA tapped Amazon Web Services to provide the back-end tools to build a live video streaming infrastructure for the Mars rover launch.
You don't often see a chief executive stand on a chair to give a presentation, but that's how "10-year-old CEO" Mark Brannigan demonstrated his 20 Questions game to the panel of judges at The London Real-Time Hackathon in April (see video below). Mark has now followed up with a glossy Coding For Kids Workbook 1: the 20 questions game, from P&M Brannigan Publishers.
Scammers are once again trying to pique Facebook users' curiosity with claims of a video that doesn't exist. The world is not ending in 2012, and NASA has not released a video saying it will.
Ever wanted to see how Qantas reconfigures a multi-deck 747 aircraft with bigger seats, fancier screens and nicer facilities? Wonder no more, with this time-lapse video from the Flying Kangaroo.
Scammers are once again using Rihanna's celebrity name to spread their nonsense on Facebook. This time, they are trying to lure you into a survey scam by telling you about a video that doesn't exist.
Windows 8 is coming this year, Windows 8 Consumer Preview will be out later this month, and the burning question is: does anybody care? ZDNet editor and long-time Linux proponent Steven J. Vaughan-Nichols claims Windows 8 will be DOA. Meanwhile, ZDNet Government and DIY-IT editor David Gewirtz (who claims all operating systems annoy him to one degree or another), claims Windows 8 is here to stay, that it matters for "real work," and so will Windows 9. In this video, originally presented as a live webcast on Feburary 15, 2012, here two opposing views on whether Windows is still relevant in a world where iPads, Android devices, and even Linux are grabbing more and more consumer attention:Discover why SJVN (as he's known to the blog-o-sphere) claims that the new Windows Metro user-interface is already stillborn and why Windows is just no longer relevant.Explore why Gewirtz (who recently vowed to move his servers back to Windows, with mixed technical results, and thousands of crazed ZDNet comments), claims that Windows is here to stay, new Microsoft UIs are often imperfect at first, and the Microsoft juggernaut will not be defeated.Learn about why the Post-PC era isn't really here yet and why most productive people can't really survive without a desktop PC or laptop.If you use computers in your business, Windows will be a factor. Don't miss this video featuring CBS Interactive's Distinguished Lecturer David Gewirtz, one of America's leading cyberdefense experts, and ZDNet Contributing Editor Steven J. Vaughan-Nichols, one of America's most-read technology journalists.Bring popcorn. Three ugly, middle-aged men argue about operating systems. It just doesn't get any better than this! Sparks will fly and there will be fireworks
An increasing number of small businesses are cashing in on the unprecedented opportunities created by the explosion of online marketing. However, an even larger number have yet to take advantage. While many might say that they don't have the time or need for such things, the growing trends arising from web-based services such as social media cannot be ignored and may ultimately determine the fate of their business. For many, the only thing that really holds them back is a clear understanding of what's possible and how to get started. Fortunately, we can help. In this video, which was originally presented as a live and interactive webcast on February 23, 2012, learn about the most important challenges, opportunities, and practical considerations for small business owners and decision makers: Learn about the most important trends that are most likely here to stay and worth your consideration; Hear how people just like you are leveraging the the web and online media to grow their business, andGain a better understanding of what you need to ensure an effective strategy for online marketing.Get it all from ZDNet Small Business Matters editor Heather Clancy who explains exactly what you need to either start or grow your budding business.


text:
(Alex Brandon ~ Associated Press)
The pumps, supplied by Moving Water Industries Corp. of Deerfield Beach, Fla., and installed at canals before the start of the 2006 hurricane season, proved to be defective, as the AP reported in March. The matter is under investigation by the Government Accountability Office, the investigative arm of Congress.
In a letter dated April 13, Sen. David Vitter, R-La., called on the Corps to look into how the politically connected company got the post-Hurricane Katrina contract. MWI employed former Florida Gov. Jeb Bush, President Bush's brother, to market its pumps during the 1980s, and top MWI officials have been major contributors to the Republican Party.
While it may not be a violation of federal regulations to adopt a company's technical specifications, it is frowned on, especially for large jobs like the MWI contract, because it could give the impression the job was rigged for the benefit of a certain company, contractors familiar with Corps practices say.
The Corps' January 2006 call for bids for 34 pumps used the wording on how the pumps should be built and tested, with minor changes, found in MWI catalogs.
The specifications were so similar that an erroneous phrase in MWI catalogs -- "the discharge tube and head assembly shall be abrasive resistance steel" -- also appears in the Corps specifications. The phrase should say "abrasion resistant steel." An incorrect reference to the type of steel that would be required apparently also was lifted.
Eugene Pawlik, a Corps spokesman in Washington, said the agency is working on a response to Vitter's letter.
MWI declined to discuss how it won the contract. GAO would not talk about its probe.
Richard White, a federal contracting expert, said it is "not unheard for a spec to be copied, in particular in cases of emergency purchases."
"It's not a good practice, but it's not anything egregious, especially if the Corps allowed other companies to negotiate to change it," White said.
After Katrina swamped about 80 percent of the city, Congress appropriated $5.7 billion to rebuild New Orleans' flood protection systems. Vitter and Sen. Mary Landrieu, D-La., have excoriated the Corps over its workmanship since Katrina.
In his letter to the commander of the Corps, Vitter said the bid solicitation for the pumps "includes specifications identical to those written and marketed by Moving Water Industries." In addition, "the testing specifications are also identical to the testing specifications developed and authored by MWI."
A May 2006 memo by a Corps inspector working on the project, provided to the AP earlier this year, warned that the pumps were faulty and would not work if needed to remove water during a hurricane. GAO opened its investigation after the memo surfaced.
The Corps and MWI insist the pumps would have worked, but last year's mild hurricane season never put them to the test. The pumps have been overhauled and are being reinstalled.


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Publications (66)341.53 Total impact
Article: The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome.
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ABSTRACT: RelE/RelB is a well-characterized toxin-anti-toxin pair involved in nutritional stress responses in Bacteria and Archae. RelE lacks any eukaryote homolog, but we demonstrate here that it efficiently and specifically cleaves mRNA in the A site of the eukaryote ribosome. The cleavage mechanism is similar to that in bacteria, showing the feasibility of A-site cleavage of mRNA for regulatory purposes also in eukaryotes. RelE cleavage in the A-site codon of a stalled eukaryote ribosome is precise and easily monitored, making "RelE printing" a useful complement to toeprinting to determine the exact mRNA location on the eukaryote ribosome and to probe the occupancy of its A site.RNA 03/2008; 14(2):233-9. · 4.62 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The foot-and-mouth disease virus (FMDV) RNA contains two in-frame AUG codons separated by 84 nt that direct translation initiation of the viral polyprotein. The mechanism of initiation at the IRES-proximal AUG codon (AUG1) has been previously analyzed, whereas no data on factor requirements for AUG2 have been reported. Here, using the method of 48S translation initiation complex reconstitution, we show that eIF1 is indispensable in forming the 48S initiation complex at AUG2. In contrast, it reduces the assembly of this complex at AUG1. Stabilization of a stem-loop between the initiation triplets induces a small decrease in the toeprint intensity at AUG2, accompanied by an increase in the AUG1/AUG2 ratio as well as a moderate reduction of protein synthesis initiated at AUG2 in transfected cells. PTB and ITAF45 exerted an additive positive effect on the 48S complex at AUG2, although a substantial reconstitution on both AUGs occurs on omission of either of these proteins. Relative to the beta-globin mRNA, the 48S complex formation at AUG1 and AUG2 is slow and occurs with the same kinetics as revealed by the "kinetic" toeprint assay. Mutation of AUG1 to AUA does not abrogate protein synthesis in transfected cells, and has no effect on the rate of the 48S complex formation at AUG2. We conclude that the AUG2 initiation region is selected independently of 48S complex formation at the upstream AUG1. The kinetic toeprint assay also shows that cap-dependent assembly of the 48S complex in vitro occurs faster than the FMDV IRES-mediated complex assembly.RNA 09/2007; 13(8):1366-74. · 4.62 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Retrotransposon L1 is a mobile genetic element of the LINE family that is extremely widespread in the mammalian genome. It encodes a dicistronic mRNA, which is exceptionally rare among eukaryotic cellular mRNAs. The extremely long and GC-rich L1 5' untranslated region (5'UTR) directs synthesis of numerous copies of RNA-binding protein ORF1p per mRNA. One could suggest that the 5'UTR of L1 mRNA contained a powerful internal ribosome entry site (IRES) element. Using transfection of cultured cells with the polyadenylated monocistronic (L1 5'UTR-Fluc) or bicistronic (Rluc-L1 5'UTR-Fluc) RNA constructs, capped or uncapped, it has been firmly established that the 5'UTR of L1 does not contain an IRES. Uncapping reduces the initiation activity of the L1 5'UTR to that of background. Moreover, the translation is inhibited by upstream AUG codons in the 5'UTR. Nevertheless, this cap-dependent initiation activity of the L1 5'UTR was unexpectedly high and resembles that of the beta-actin 5'UTR (84 nucleotides long). Strikingly, the deletion of up to 80% of the nucleotide sequence of the L1 5'UTR, with most of its stem loops, does not significantly change its translation initiation efficiency. These data can modify current ideas on mechanisms used by 40S ribosomal subunits to cope with complex 5'UTRs and call into question the conception that every long GC-rich 5'UTR working with a high efficiency has to contain an IRES. Our data also demonstrate that the ORF2 translation initiation is not directed by internal initiation, either. It is very inefficient and presumably based on a reinitiation event.Molecular and Cellular Biology 08/2007; 27(13):4685-97. · 5.04 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Similar features in the mechanisms of mRNA translation initiation on prokaryotic and eukaryotic ribosomes are discussed with examples from mRNAs with nonstandard 5′-untranslated regions (5′-UTRs) and mRNAs lacking 5′-UTR (leaderless mRNAs).Molecular Biology 06/2006; 40(4):620-627. · 0.74 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Translation initiation in eukaryotic cells is known to be a complex multistep process which involves numerous protein factors. Here we demonstrate that leaderless mRNAs with initiator Met-tRNA can bind directly to 80S mammalian ribosomes in the absence of initiation factors and that the complexes thus formed are fully competent for the subsequent steps of polypeptide synthesis. We show that the canonical 48S pathway of eukaryotic translation initiation has no obvious advantage over the 80S pathway of translation initiation on leaderless mRNAs and suggest that, in the presence of competing mRNAs containing a leader, the latter mechanism will be preferred. The direct binding of the leaderless mRNA to the 80S ribosome was precluded when such an mRNA was supplied with a 5' leader, irrespective of whether it was in a totally single-stranded conformation or was prone to base pairing. The striking similarity between the mechanisms of binding of leaderless mRNAs with mammalian 80S or bacterial 70S ribosomes gives support to the idea that the alternative mode of translation initiation used by leaderless mRNAs represents a relic from early steps in the evolution of the translation apparatus.Molecular and Cellular Biology 05/2006; 26(8):3164-9. · 5.04 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Binding of the internal ribosome entry site (IRES) of the hepatitis C virus (HCV) RNA to the eIF-free 40S ribosomal subunit is the first step of initiation of translation of the viral RNA. Hairpins IIId and IIIe comprising 253-302 nt of the IRES are known to be essential for binding to the 40S subunit. Here we have examined the molecular environment of the HCV IRES in its binary complex with the human 40S ribosomal subunit. For this purpose, two RNA derivatives were used that bore a photoactivatable perfluorophenyl azide cross-linker. In one derivative the cross-linker was at the nucleotide A296 in hairpin IIIe, and in the other at G87 in domain II. Site-specific introduction of the cross-linker was performed using alkylating derivatives of oligodeoxyribonucleotides complementary to the target RNA sequences. No cross-links with the rRNA were detected with either RNA derivative. The RNA with the photoactivatable group at A296 cross-linked to proteins identified as S5 and S16 (major) and p40 and S3a (minor), while no cross-links with proteins were detected with RNA modified at G87. The results obtained indicate that hairpin IIIe is located on the solvent side of the 40S subunit head on a site opposite the beak.Nucleic Acids Research 02/2006; 34(7):2027-36. · 8.81 Impact Factor -
Article: A cross-kingdom internal ribosome entry site reveals a simplified mode of internal ribosome entry.
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ABSTRACT: Rhopalosiphum padi virus (RhPV) is an insect virus of the Dicistroviridae family. Recently, the 579-nucleotide-long 5' untranslated region (UTR) of RhPV has been shown to contain an internal ribosome entry site (IRES) that functions efficiently in mammalian, plant, and insect in vitro translation systems. Here, the mechanism of action of the RhPV IRES has been characterized by reconstitution of mammalian 48S initiation complexes on the IRES from purified components combined with the toeprint assay. There is an absolute requirement for the initiation factors eIF2 and eIF3 and the scanning factor eIF1 to form 48S complexes on the IRES. In addition, eIF1A, eIF4F (or the C-terminal fragment of eIF4G), and eIF4A strongly stimulated the assembly of this complex, whereas eIF4B had no effect. Although the eIF4-dependent pathway is dominant in the RhPV IRES-directed cell-free translation, omission of either eIF4G or eIF4A or both still allowed the assembly of 48S complexes from purified components with approximately 23% of maximum efficiency. Deletions of up to 100 nucleotides throughout the 5'-UTR sequence produced at most a marginal effect on the IRES activity, suggesting the absence of specific binding sites for initiation factors. Only deletion of the U-rich unstructured 380-nucleotide region proximal to the initiation codon resulted in a complete loss of the IRES activity. We suggest that the single-stranded nature of the RhPV IRES accounts for its strong but less selective potential to bind key mRNA recruiting components of the translation initiation apparatus from diverse origins.Molecular and Cellular Biology 10/2005; 25(17):7879-88. · 5.04 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Initiation of protein synthesis on picornavirus RNA requires an internal ribosome entry site (IRES). Typically, picornavirus IRES elements contain about 450 nucleotides (nt) and use most of the cellular translation initiation factors. However, it is now shown that just 280 nt of the porcine teschovirus type 1 Talfan (PTV-1) 5' untranslated region direct the efficient internal initiation of translation in vitro and within cells. In toeprinting assays, assembly of 48S preinitiation complexes from purified components on the PTV-1 IRES was achieved with just 40S ribosomal subunits plus eIF2 and Met-tRNA(i)(Met). Indeed, a binary complex between 40S subunits and the PTV-1 IRES is formed. Thus, the PTV-1 IRES has properties that are entirely different from other picornavirus IRES elements but highly reminiscent of the hepatitis C virus (HCV) IRES. Comparison between the PTV-1 IRES and HCV IRES elements revealed islands of high sequence identity that occur in regions critical for the interactions of the HCV IRES with the 40S ribosomal subunit and eIF3. Thus, there is significant functional and structural similarity between the IRES elements from the picornavirus PTV-1 and HCV, a flavivirus.Journal of Virology 06/2004; 78(9):4487-97. · 4.65 Impact Factor - Molecular Biology 03/2004; 38(2):261-265. · 0.74 Impact Factor
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ABSTRACT: The reconstitution of translation initiation complexes from purified components is a reliable approach to determine the complete set of essential canonical initiation factors and auxiliary proteins required for the 40S ribosomal subunit to locate the initiation codon on individual mRNAs. Until now, it has been successful mostly for formation of 48S translation initiation complexes with viral IRES elements. Among cap-dependent mRNAs, only globin mRNAs and transcripts with artificial 5' leaders were amenable to this assembly. Here, with modified conditions for the reconstitution, 48S complexes have been successfully assembled with the 5' UTR of beta-actin mRNA (84 nucleotides) and the tripartite leader of adenovirus RNAs (232 nucleotides), though the latter has been able to use only the scanning rather then the shunting model of translation initiation with canonical initiation factors. We show that initiation factor 4B is essential for mRNAs that have even a rather moderate base pairing within their 5' UTRs (with the cumulative stability of the secondary structure within the entire 5' UTR < -13 kcal/mol) and not essential for beta-globin mRNA. A recombinant eIF4B poorly substitutes for the native factor. The 5' UTRs with base-paired G residues reveal a very sharp dependence on the eIF4B concentration to form the 48S complex. The data suggest that even small variations in concentration or activity of eIF4B in mammalian cells may differentially affect the translation of different classes of cap-dependent cellular mRNAs.Molecular and Cellular Biology 12/2003; 23(24):8925-33. · 5.04 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: An approach based on complementary addressed modification of nucleic acids by oligodeoxyribonucleotide derivatives was proposed for changing the spatial structure of particular RNA sites in order to study their role in the biological activity of the total RNA molecule. Hepatitis C virus (HCV) IRES was used as a model. Oligodeoxyribonucleotide derivatives contained a 4-[N-(2-chloroethyl)-N-methylamino]benzylamino group at the 5-phosphate and were complementary to various RNA sites located in regions of hairpins II, IIId, or IIIe. Covalent adducts resulting from RNA alkylation with the derivatives were isolated by denaturing PAGE and tested for binding with the 40S subunit of human ribosomes. Structural alteration of hairpin II had no effect on the binding, whereas that of hairpin IIIe substantially reduced it. The RNA with modified hairpin IIId showed virtually no binding with the 40S subunit. Hairpin IIId was assumed to play the critical role in the binding of HCV IRES with the 40S subunit.Molecular Biology 10/2003; 37(6):873-879. · 0.74 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Human immunodeficiency virus type 1 (HIV) gag/pol and env mRNAs contain cis-acting regulatory elements (INS) that impair stability, nucleocytoplasmic transport, and translation by unknown mechanisms. This downregulation can be counteracted by the viral Rev protein, resulting in efficient export and expression of these mRNAs. Here, we show that the INS region in HIV-1 gag mRNA is a high-affinity ligand of p54nrb/PSF, a heterodimeric transcription/splicing factor. Both subunits bound INS RNA in vitro with similar affinity and specificity. Using an INS-containing subgenomic gag mRNA, we show that it specifically associated with p54nrb in vivo and that PSF inhibited its expression, acting via INS. Studying the authentic HIV-1 mRNAs produced from an infectious molecular clone, we found that PSF affected specifically the INS-containing, Rev-dependent transcripts encoding Gag-Pol and Env. Both subunits contained nuclear export and nuclear retention signals, whereas p54nrb was continuously exported from the nucleus and associated with INS-containing mRNA in the cytoplasm, suggesting its additional role at late steps of mRNA metabolism. Thus, p54nrb and PSF have properties of key factors mediating INS function and likely define a novel mRNA regulatory pathway that is hijacked by HIV-1.Molecular and Cellular Biology 10/2003; 23(18):6618-30. · 5.04 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A relaxed cap-dependence of translation of the mRNA-encoding mammalian heat shock protein Hsp70 may suggest that its 5'-untranslated region (UTR) possesses an internal ribosome entry site (IRES). In this study, this possibility has been tested in transfected cells using plasmids that express dicistronic mRNAs. Using a reporter gene construct, Renilla luciferase/Photinus pyralis luciferase, we show that the 216-nt long 5'-UTR of Hsp70 mRNA acts as an IRES that directs ribosomes to the downstream start codon by a cap-independent mechanism. The relative activity of this IRES (100-fold over the empty vector) is similar to that of the classical picornaviral IRESs. Additional controls indicate that this high expression of the downstream reporter is not due to readthrough from the upstream cistron, nor is it due to translation of cryptic monocistronic transcripts. The effect of small deletions within the 5'-UTR of Hsp70 mRNA on the IRES activity varies in dependence on their position within the 5'-UTR sequence. With the exception of deletion of nt 33-50, it is small for the 5'-terminal half of the 5'-UTR and rather strong for the 3'-terminal section. However, neither of these small deletions abolishes the IRES activity completely. Excision of larger sections (>50 nt) by truncation of the 5'-UTR from the 5'-end or by internal deleting results in a dramatic impairment of the IRES function. Taken together, these data suggest that the IRES activity of the 5'-UTR of Hsp70 mRNA requires integrity of almost the entire sequence of the 5'-UTR. The data are discussed in terms of a model that allows a three-dimensional rather than linear mode of selection of the initiation region surrounding the start codon of Hsp70 mRNA.Journal of Biological Chemistry 06/2003; 278(25):22350-6. · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A method of analysis of translation initiation complexes by toeprinting has recently acquired a wide application to investigate molecular mechanisms of translation initiation in eukaryotes. So far, this very fruitful approach was used when researchers did not aim to discriminate between patterns of toeprints for 48S and 80S translation initiation complexes. Here, using cap-dependent and internal ribosomal entry site (IRES)-dependent mRNAs, we show that the toeprint patterns for 48S and 80S complexes are distinct whether the complexes are assembled in rabbit reticulocyte lysate or from fully purified individual components. This observation allowed us to demonstrate for the first time a delay in the conversion of the 48S complex into the 80S complex for beta-globin and encephalomyocarditis virus (EMCV) RNAs, and to assess the potential of some 80S antibiotics to block polypeptide elongation. Besides, additional selection of the authentic initiation codon among three consecutive AUGs that follow the EMCV IRES was revealed at steps subsequent to the location of the initiation codon by the 40S ribosomal subunit.FEBS Letters 02/2003; 533(1-3):99-104. · 3.34 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Nucleotide sequence changes increasing the number of paired bases without producing stable secondary structure in the 5"-untranslated region (5"-UTR) of the -globin mRNA had a slight effect on its translation in rabbit reticulocyte lysate at low mRNA concentration and dramatically decreased translation efficiency at a high concentration. The removal of paired regions restored translation. Addition of purified eIF2 to the lysate resulted in equal translation efficiencies of templates differing in structure of 5"-UTR. A similar effect was observed for p50, a major mRNP protein. Other mRNA-binding initiation factors, eIF4F and eIF4B, had no effect on the dependence of translation efficiency on mRNA concentration. Analysis of the assembly of the 48S initiation complex from its purified components showed that less eIF2 is required for translation initiation on the -globin mRNA than on its derivative containing minor secondary structure elements in 5"-UTR. According to a model proposed, eIF2 not only delivers Met-tRNA, but it also stabilizes the interaction of the 40S ribosome subunit with 5"-UTR, which is of particular importance for translation initiation on templates with structured 5"-UTR.Molecular Biology 01/2003; 37(3):421-428. · 0.74 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: p50, the major core protein bound to mammalian mRNAs, has been reported to stimulate translation at low p50/mRNA ratios and inhibit translation at high p50/mRNA ratios. This study aims to address the molecular mechanisms underlying these phenomena using the in vitro assembly of 48 S preinitiation complexes from fully purified translational components in the presence or absence of p50 as analyzed by the toeprint assay. With limited concentrations of eIF2, eIF3, and eIF4F, p50 (but not pyrimidine tract-binding protein, which was taken for comparison) strongly stimulates formation of the 48 S preinitiation complexes with beta-globin mRNA. This stimulation is observed when just a few molecules of p50 are bound per molecule of the mRNA. When the amount of p50 in solution is increased over some threshold p50/mRNA ratio, a remarkable repression is observed that can still be relieved by adding more eIF2 and eIF4F. At even higher concentrations of p50, the inhibitory effect becomes irreversible. The threshold ratio depends upon the extent of secondary structure of the 5'-untranslated region linked to the beta-globin coding region. Chemical probing has confirmed that the binding of p50 to mRNA involves only the sugar-phosphate backbone of the mRNA leaving nucleotide bases free for interaction with other messenger ribonucleoprotein (mRNP) components. These data are best compatible with the functional role of p50 as a "manager" of mRNA-protein interactions in mammalian mRNPs.Journal of Biological Chemistry 06/2002; 277(18):15445-51. · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Translation initiation is a complex process in which initiator tRNA, 40S, and 60S ribosomal subunits are assembled by eukaryotic initiation factors (eIFs) into an 80S ribosome at the initiation codon of mRNA. The cap-binding complex eIF4F and the factors eIF4A and eIF4B are required for binding of 43S complexes (comprising a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped mRNA but are not sufficient to promote ribosomal scanning to the initiation codon. eIF1A enhances the ability of eIF1 to dissociate aberrantly assembled complexes from mRNA, and these factors synergistically mediate 48S complex assembly at the initiation codon. Joining of 48S complexes to 60S subunits to form 80S ribosomes requires eIF5B, which has an essential ribosome-dependent GTPase activity and hydrolysis of eIF2-bound GTP induced by eIF5. Initiation on a few mRNAs is cap-independent and occurs instead by internal ribosomal entry. Encephalomyocarditis virus (EMCV) and hepatitis C virus epitomize distinct mechanisms of internal ribosomal entry site (IRES)-mediated initiation. The eIF4A and eIF4G subunits of eIF4F bind immediately upstream of the EMCV initiation codon and promote binding of 43S complexes. EMCV initiation does not involve scanning and does not require eIF1, eIF1A, and the eIF4E subunit of eIF4F. Initiation on some EMCV-like IRESs requires additional noncanonical initiation factors, which alter IRES conformation and promote binding of eIF4A/4G. Initiation on the hepatitis C virus IRES is even simpler: 43S complexes containing only eIF2 and eIF3 bind directly to the initiation codon as a result of specific interaction of the IRES and the 40S subunit.Proceedings of the National Academy of Sciences 07/2001; 98(13):7029-36. · 9.81 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: 15-lipoxygenase (LOX) expression is translationally silenced in early erythroid precursor cells by a specific mRNA-protein complex formed between the differentiation control element in the 3' untranslated region (UTR) and hnRNPs K and E1. The 3'UTR regulatory complex prevents translation initiation by an unknown mechanism. We demonstrate that the 40S ribosomal subunit can be recruited and scan to the translation initiation codon even when the silencing complex is bound to the 3'UTR. However, the joining of the 60S ribosomal subunit at the AUG codon to form a translation competent 80S ribosome is inhibited, unless initiation is mediated by the IGR-IRES of the cricket paralysis virus. These findings identify the critical step at which LOX mRNA translation is controlled and reveal that 60S subunit joining can be specifically regulated.Cell 02/2001; 104(2):281-90. · 33.12 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Papers on the mechanisms of translation initiation in mammals studied by reconstruction of initiation complexes from individual components are reviewed. The author points to the constraints of this approach and to the pitfalls ignoring which one might come to erroneous conclusions and even artifacts. In addition, some methods employed in the field as well as some technical problems are discussed in the paper, together with the means of obviating them. The review could be a guidebook for newcomers into this quite labor-consuming field.Molecular Biology 01/2001; 35(4):536-543. · 0.74 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A complex of eukaryotic initiation factors (eIFs) 4A, 4E, and 4G (collectively termed eIF4F) plays a key role in recruiting mRNAs to ribosomes during translation initiation. The site of ribosomal entry onto most mRNAs is determined by interaction of the 5'-terminal cap with eIF4E; eIFs 4A and 4G may facilitate ribosomal entry by modifying mRNA structure near the cap and by interacting with ribosome-associated factors. eIF4G recruits uncapped encephalomyocarditis virus (EMCV) mRNA to ribosomes without the involvement of eIF4E by binding directly to the approximately 450-nucleotide long EMCV internal ribosome entry site (IRES). We have used chemical and enzymatic probing to map the eIF4G binding site to a structural element within the J-K domain of the EMCV IRES that consists of an oligo(A) loop at the junction of three helices. The oligo(A) loop itself is not sufficient to form stable complexes with eIF4G since alteration of its structural context abolished its interaction with eIF4G. Addition of wild type or trans-dominant mutant forms of eIF4A to binary IRES.eIF4G complexes did not further alter the pattern of chemical/enzymatic modification of the IRES.Journal of Biological Chemistry 08/1998; 273(29):18599-604. · 4.60 Impact Factor
Publication Stats
3k | Citations | |
341.53 | Total Impact Points | |
Top Journals
Institute of Chemical Biology and Fundamental Medicine
Novo-Nikolaevsk, Novosibirsk, Russia
Lomonosov Moscow State University
- A. N. Belozersky Research Institute of Physico-Chemical Biology
Moskva, Moscow, Russia
Moscow State Textile University
Moskva, Moscow, Russia
Институт молекулярной биологии им. В.А. Энгельгардта Российской академии наук
Moskva, Moscow, Russia
Case Western Reserve University School of Medicine
- Department of Biochemistry
Cleveland, Ohio, United States
Russian Academy of Sciences
- Institute of Inorganic Chemistry
Moskva, Moscow, Russia