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Hostas love shade. There are lots of posts and lots of lists about Hostas that do well in lots of sun. But what about Hostas that do well in deep shade? I thought I'd start a thread just for them. I have a treed hillside that gets dappled sun all day. I tried to establish a few large Hostas 3 years ago, and to my dismay several of them appear to shrink each year, either coming back with fewer eyes or smaller leaves than the previous year. What's shrinking: What's doing well: I hope to add more (large, very large, giant) Hostas every year, but I don't want them to to fade away. What are your experiences with Hostas that get very little direct sunlight? Do you have some that do especially well in deep shade? |
Follow-Up Postings:
- Posted by squirejohn 4 (My Page) on Sun, Jul 27, 14 at 11:20
I wonder if some of the shade difference may be attributed to tree roots and/or a specific location where some hostas get direct sun via a hole in the canopy. There is a photo of Gold Standard in Grenfell's Encyclopedia and Field Guide that shows a GS in deep shade. I tried to achieve that same color by putting GS in deep shade that gets no direct sun without success - it looks no different than the rest of my GS's. |
- Posted by ken_adrian MI z5 (My Page) on Sun, Jul 27, 14 at 17:02
john stole my line... what are the trees ... and secondly.. how do you water on a hill.. and insure the water soaks in.. say .... 6 inches.. instead of running down hill??? .. i have found it near impossible ... lastly... if that is your woods in the pic .. and you are going to stay there for decades... you ought to have the trees thinned ... BEFORE YOU PUT THE GARDEN IN... and by that i mean.. without being there.. removing everything under say.. 4 inches ... you might be surprised... how by removing the under canopy.. how all of a sudden ... you have better light ... snow cap is not a good plant except for 2 peeps here.. who mock me.. lol ... its carp ... and BDaddy is about the same for me ... i dont think it likes my sand.. which begs the ???.. whats your soil type ... not being able to grow GS there.. is a mystery ... in fact.. if you found that post on landscaping with GS... you could see what your hill might look like.. with the smaller trees removed.... i would try again.. with a bigger GS ... ken |
Marta and Ken. I notice you both have problems with Big daddy. So do I. Out of all the ones I planted only one is managing to survive and its not very big. The other ones that really don't seem to do well with shade for me is the empress wu. I do have three growing now but have tried before and all have not survived. Maybe its because the plants were too small who knows. I do garden under pine trees but you have to be very very good at watering the plants, and even then the hostas there don't get overly big. This is where the unnamed ones and doubles are going. michelle |
- Posted by Marta_in_QC 4b (My Page) on Wed, Jul 30, 14 at 18:11
Thanks for all the good input. squirejohn and ken_adrian, your posts are completely on-target. I have already learned more from a few weeks reading this forum than the last few years of trial and error. I realize that there are other factors in this environment than deep shade. I also have to consider root competition, soil, water, and pests/critters. This year also we had an exceptionally cold, long winter, with a very late, short, and cold spring. Yes, it's my hillside, and yes, I plan to be here for decades. I just read the Seven Levels of Hosta Gardeners thread, and Level 3 describes my situation pretty much exactly. :o) In a nutshell, my plans for Hosta Hill should get me through Level 4 without loss of lawn. My plan was to get a few very large/giant Hostas to start growing while I figure out how to do the rest... The idea is this: I establish a walkable, meandering foot path that snakes up the hill. Maybe put some stepping stones between loops (think chutes and ladders). I also want to stack up some low rock walls to make terraces, fill with soil, and plant lots more Hostas, probably about 50-100 per year. I estimate this will take 10+ years (I'm not retired and this is only a part of my garden). I'm not in a hurry, but I do want those giant Hostas to get growing, not fade away! :o) The trees are mostly beech, with a mix of birch/aspen, oak, and maple. I will take Ken's advice and remove all small-diameter trees before planting more Hostas. I hadn't heard of spinout bags until I found this forum. I like the idea, but I wonder how big the spinout bags would need to be to accommodate the rootball of a mature giant Hosta... I'd probably have to make my own. I'm no expert, but I believe the hillside holds moisture fairly well. The soil is mostly clay, with a good layer of decomposing leaves. There is always some ground vegetation, mushrooms pop up regularly, and a few weeks ago I even found some amazing Monotropa uniflora (Indian Pipe) growing there. Watering the hill by hand would be a pain, but thankfully my region gets a lot of rainfall, as well as spring run-off of melting snow (we are at the foot of a hilly provincial park - this comes with some wildlife challenges too). I had put mulch around the first Hostas, but I stopped that because I read it encourages voles, and I found holes around the roots of several Hostas, here and elsewhere. I have not done anything other than soak those roots with a water, castor oil, and soap mixture. The Gold Standard actually looks great, but it's still about the same size as when I planted it 2 years ago. I planted another one in a different part of the garden last year, and the newer one is almost double the size of the older one. Maybe I just need to be patient. I've heard of Hostas that seem to stall for a few seasons and then take off. I relocated Snow Cap to another spot. Both of its scrawny petioles were broken, but the leaves were still attached (possibly a deer stepped on it). I figured it for a goner, but the root ball seemed good, and Snow Cap is putting out some new leaves and looks like it's going to recover. I relocated Big Daddy to another spot too, but the recovery isn't as dramatic as with Snow Cap. Maybe I need to get him a Big Mama to get him to perk up. :o) Here is another photo of my fledgling Hosta Hill. The only Hosta that you can make out (to the right of the bench) is Wide Brim. I guess a bit of brush clearing is in order. |
- Posted by josephines67 5 ON (My Page) on Wed, Jul 30, 14 at 18:50
Too bad you live so far away! What I'd do to clear brush in that gorgeous environment!! Sounds like you have a good plan in place. Have fun! One deep shade lover is Dark Star (blue/green with ivory margin) but it is S-M, increases rapidly, providing excellent ground cover. It might be a good plant to use for massing as a natural edge to the area. |
Wow Josephine dark star is nice!! Marta! There is so much potential there! You will have fun! I think also do as Ken suggested and do some clearing. Whats in front of the bench?? Its looks like downed trees or logs or something? This picture is taken at your house facing backyard?? |
- Posted by ken_adrian MI z5 (My Page) on Thu, Jul 31, 14 at 8:05
you do not have to dig up the trees you cut down... cut to the ground.. and apply 100% RUp to the cambian layer of the stump ... and be done with that part ... and stripped trunks of those trees.. can come in handy ... i used to use such as path edging ... ken |
We dug up a maple stump and made such a huge hole that it is now the Komodo Dragon bed. Kathy |
- Posted by josephines67 5 ON (My Page) on Thu, Jul 31, 14 at 10:26
Thanks, Hostacats! :-) |
Another thing you can do is to build some pergolas. I have three in my yard. Depending upon how close you put the roof slats, you get differing amounts of shade. You do not have to deal with tree roots, and you could put drip water system if you want. The basic 12x12 pergola requires a mix of 4x4s, 2x10s, 2x8s and 2x6s with varying amounts of bolts, some roofing slats, screws and a bag of quick crete to secure the 4x4s in the ground. I can cram at least 2 dozen hostas underneath and then I usually go out at least 18 inches on each side for even more garden space. And, you can put the pergola in full sun and still get shade. I usually use a screwdriver as the spacer for the roof slats. As the wood dries, it ends up being about a half inch between the slats, allowing both sun and shade. And no tree roots. Materials $200-$500 plus your time A nice weekend project for the DIY-er. Note: (I like to bolt two of the 2x8s to two 4x4s (one inside and one outside of the 4x4) (two sets of these) before I dig the holes. Makes the job easier, although you may need a buddy to help hoist the 4x4 and 2x8 frame into the holes. dave |
Be careful when you write : "Hostas that do well in lots of sun. ". That is only true when you give them an equal and consistent LOTS OF WATER. The moment you forget to water they will burn and shrink!! |
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Lost in Downtown Brooklyn
Jun 13, 2012 by fatschmat in Outer Boroughs
Any ideas for a dinner for 50 people in Central Paris ideally within 2km of the Eiffel Tower
Jun 18, 2012 by parmoor in France
41 Degrees Website
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Paris - lunch planning: reservations vs. flying by the seat of my pants
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San Sebastian: Bodegon Alejandro-style options
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Any suggestions for dinner in San Sebastían on a MONDAY night?
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NY hound visiting San Francisco. Must Try Foods?
Mar 28, 2012 by agaaga in San Francisco Bay Area
South Bay - giantic to-do list in progress
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Intergalactic foodies
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In dire need of an East Coast Jewish Deli!
Feb 29, 2012 by randej in San Francisco Bay Area
Mid-priced dinner in interesting/scenic area for European friends
Feb 07, 2012 by waxyjax in San Francisco Bay Area
One night in brooklyn?
Jan 09, 2012 by lnedrive14 in Outer Boroughs
Food foreigners take back home when they visit America.
Jun 07, 2011 by Monica in General Topics
Dinner suggestions in Williamsburg this Saturday night
Sep 28, 2011 by olivia7261 in Outer Boroughs
Am I crazy for thinking that Magnolia cupcakes suck?
Jan 20, 2008 by meldrom in Manhattan
TASTE Williamsburg Greenpoint today
Sep 18, 2011 by waxyjax in Outer Boroughs
Where to take NYU student?
Sep 10, 2011 by mocro in Manhattan
Special Occasion Greek: Avra, Pylos, Periyal or Milos?
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Buttermilk CHannel and others
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M. Wells Dinner
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Last Minute anniverary in BK (or even Manhattan)
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Petrelle report
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Best Fried Chicken in Manhattan
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Boerum Hill
Best meal I ever ate:
anytime my mom feels like showing off
My current drink of choice:
rooibos, orange juice, Lagavulin, extra special arrack from Sri Lanka, red from Navarra, white from New Zealand, rose from Catalunya, throat comfort tea after a long night of partying, coconut water on a hot day
I really wish I could:
have some sort of thyroid problem that necessitated me eating 5 meals a day
My "Go-to" dinner party dish:
risotto, fondue or summer rolls
My favorite comfort food:
My top 5 favorite restaurants:
is another unfair question...but at the very least I'll take this opportunity to brag about dining at El Bulli and Noma
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If the Internet were a book I would've definitely torn it up by now...same goes for The Complete Pepin on PBS
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...descriptors to share a bit of our long weekend:
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Shot entirely within Hartman Rocks rec area near Gunnison, Colorado.
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Dr. Mary V. Kopke, MD
Internal Medicine, Mount Sinai Visiting Doctors
- 1468 Madison Ave
New York, NY 10029 - 1 Office Location
- (212) 241-4141
Dr. Mary V. Kopke graduated from the George Washington University School of Medicine and Health Science in 2008. She works in New York, NY and specializes in Internal Medicine. Dr. Kopke is affiliated with Mount Sinai Medical Center. She speaks Chinese, English and Spanish.
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Fall 2011 Street Style - How to Wear Neon For Fall
Neon Lights
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>> Here's an unexpected color combination that looks just right for early Fall. Neon lime and cherry red may seem a bit unorthodox, but this street-snapped ensemble is all about balance — a black trench and earthy-toned booties temper the vividness of the bag and dress, while a smear of red lipstick brings the whole thing together. Click through for similar finds to make this super-chic look your own.
Photo courtesy of Phil Oh
Street Style
Link Love
- Kanye West might be making over the Washington Wizards! — HuffPost Style
- Fact: you don't need to wear heels to look taller — SHEFinds
- J Lo's wardrobe is a lot more straightforward than you'd think — Vulture
- Make your bag look fancy-shmancy, even if it's not — Who What Wear
- Isabel Toledo for Lane Bryant is back in full force — InStyle
- When your street style's geometric, you're in good shape — StyleList
- All about Kristen Stewart's outfits? Learn about her favorite label — Style.com
- Are you a basic fashion girl for wearing these brands? — Fashionista
- Why Vince Camuto was a true design master — Racked
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IT & Telecom
January 30, 2015
The UAE currently uses drones for official purposes such as to deliver documents and packages to its citizens as part of its efforts to upgrade government services.
January 29, 2015
Facebook posted a fourth-quarter revenue of $3.85 billion up 49 per cent from $2.59 billion in the year-ago period.
When HTC, a global leader in mobile innovation and design, first debuted its highly-anticipated Desire line-up – a remarkable roster of mid-range smartphones specially built to cater to all customer segments –legions of consumers rejoiced.
· His Highness Sheikh Hamdan bin Mohammed bin Rashid Al Maktoum, Crown Prince of Dubai, honours du with his support of #Narzif
Canon Middle East, a leader in imaging solutions, and Canon Emirates organised a joint kick-off meeting at Waldorf Astoria The Palm in Dubai
Despite the high price, it is not the most costliest phone in the world.
January 28, 2015
The company sold 74.5 million iPhones in its fiscal first quarter ended Dec. 27, while many analysts had expected fewer than 70 million.
NetApp, Inc. (NASDAQ: NTAP) has announced the NetApp® EF560 all-flash array with improvements in storage performance to offer the absolute and consistent latency, bandwidth and IOPS critical to enterprise database and analytics applications.
Sony's new α7 II (ILCE-7M2) is the latest in the α7 series of interchangeable-lens models and the world's first[1] camera with optical 5-axis image stabilisation designed for a full-frame image sensor.
Today's net generation wants more freedom, flexibility, empowerment to control their work life
January 27, 2015
Under the banner "Creating Possibilities, Shaping the Future," Samsung Electronics Co., Ltd., unveiled its vision for smarter living at the company's 2015 International Consumer Electronics Show (CES) press conference.
Ericsson today announced that it was a winner across four categories at the Compass Intelligence Awards ceremony held during the Consumer Electronics Show in Las Vegas on January 7.
January 26, 2015
LG Electronics (LG) continues to push the industry boundaries with the unveiling of the new G Flex2 at the 2015 International CES® in Las Vegas.
Ericsson announced today that Tarek Saadi has been appointed Executive Vice President and Head of Sales for Ericsson in the Middle East region.
January 25, 2015
The Yokne'am-based company, Amazon's first Israeli acquisition, is building an innovative semiconductor platform.


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2014's fast food atrocities
Burger King's black cheeseburger: Made with squid ink and bamboo charcoal, arguably a symbol of meat's destructive effect on the planet. Only available in Japan.
Topics: Sex, United Nations, Sex Work, Love and Sex, News
Two former employees of DynCorp, the government contracting powerhouse, have won legal victories after charging that the $2 billion-a-year firm fired them when they complained that co-workers were involved in a Bosnia sex-slave trade.
The court actions — one in the United Kingdom, the other in Fort Worth, Texas — suggest that the company did not move aggressively enough when reports of sexual misconduct among its employees began to emerge in 1999. The tribunal in the U.K. found that DynCorp employee Kathryn Bolkovac "acted reasonably," but that the company did not.
"DynCorp is an enormous operation, with strong ties to the U.S. government," Bolkovac's legal representative, Karen Bailey, said in a prepared statement. "She took on the big guns and won. The plight of trafficking victims is appalling and I'm glad that Kathryn's case has gone some way to bringing it to wider attention."
The tribunal found that DynCorp Aerospace UK Ltd., a subsidiary of DynCorp Inc., violated the U.K.'s whistle-blowing statute — the Public Interest Disclosure Act of 1998 — when the company fired Bolkovac. A separate hearing is scheduled for October to determine what damages DynCorp should face.
DynCorp did not respond to calls seeking comment on Monday. But in remarks to the Associated Press, DynCorp spokesman Chuck Taylor said the company was considering an appeal. "We're very disappointed in the tribunal's ruling and can only reinforce that DynCorp's decision to dismiss Ms. Bolkovac was based solely on the grounds of gross misconduct because of time-sheet fraud," Taylor said.
In the second case, DynCorp agreed to settle a suit brought by former helicopter mechanic Ben Johnston late Friday night, two days before the case was set to go to trial in Texas. The amount of Johnston's settlement is confidential, but both Johnston and his attorney said they viewed the settlement as a victory — and as a vindication after two years of fighting the company.
"This settlement wouldn't have happened if DynCorp hadn't, at least internally, accepted some responsibility for what happened in the Balkans," said Johnston's attorney, Kevin Glasheen, when reached at his Lubbock, Texas, office.
In late June, Salon published a two-part investigation into the participation of DynCorp employees in the Bosnian sex-slave trade, based in part on evidence uncovered in the Johnston case. At least 13 DynCorp employees have been sent home from Bosnia — and at least seven of them fired — for purchasing women or participating in other prostitution-related activities. But despite large amounts of evidence in some cases, none of the DynCorp employees sent home have faced criminal prosecution.
Because of a combination of international treaties, jurisdictional loopholes and bureaucratic confusion, employees of private military companies such as DynCorp can escape prosecution for crimes they commit overseas. Most common crimes committed outside the United States are beyond the jurisdiction of U.S. courts, and the burgeoning local law enforcement systems in war-torn regions such as Bosnia are often insufficient or unwilling to police U.S. contractors.
Forced prostitution is common in nations undergoing rebuilding, due in large part to the massive contingent of unaccompanied, highly paid, mostly male international aid workers sent to such countries, according to human rights workers. Martina Vandenberg, a women's rights researcher with Human Rights Watch, told Salon that during a 1999 tour, she found that "Bosnia was absolutely littered with brothels" staffed by women who had been sold as chattel for $600 to $700, "with all the rights of ownership attaching."
DynCorp is a privately held company that relies on government contracts for over 95 percent of its business. Among other services, it provides pilots to the State Department; maintenance crews, communications specialists and weapons experts to the armed forces; and police officers to the U.N.
Ben Johnston claims that buying prostitutes — many of whom were clearly underage — had become so common among DynCorp employees at Camp Comanche, outside Tuzla, Bosnia, that he was forced to report the problem to the U.S. Army's Criminal Investigative Command.
The Army conducted an investigation into Johnston's claims and eventually compiled a significant amount of evidence implicating at least two DynCorp employees in wrongdoing involving local prostitutes. The freedom of the prostitutes was never positively determined, as investigators ultimately found that the Army did not have jurisdiction over civilian contractors and turned the case over to the Bosnian police. The Bosnian police, who were unsure whether DynCorp employees were immune from Bosnian jurisdiction under the Dayton Peace Accords, never brought charges against the men. They were sent home and fired by DynCorp after the Army reported its findings to the company.
Johnston was also fired by DynCorp at that time. Glasheen, his attorney, said he is confident that they would have won a jury trial, in part because DynCorp's excuses for firing Johnston were so numerous and varied that they undermined the company's case. The company's official reason for firing Johnston was that he had "brought discredit to the company and the U.S. Army." On its Web site, DynCorp stated that Johnston's firing had nothing to do with the Army investigation and that the company didn't even know he had complained to the Criminal Investigative Command. The manager who signed his termination papers, however, stated in a deposition that Johnston had brought discredit to the company and the Army with unsubstantiated claims against co-workers — even though Army investigators had found support for some of Johnston's allegations. Various other claims by DynCorp supervisors alternately implied that Johnston was fired for poor workmanship, misuse of company assets, or failing to report to work. Johnston denies all of these charges.
DynCorp had contended that Bolkovac was fired for falsifying time sheets. But in her case, she says, DynCorp fired her after she sent an e-mail to DynCorp and U.N. higher-ups describing complicity in forced prostitution by international aid workers, including members of the International Police Task Force. The British employment tribunal supported Bolkovac's version of events, finding little to support DynCorp's claim and describing the evidence DynCorp provided as being "sketchy to the point of nonexistent," according to Bolkovac's attorneys.
Burger King's black cheeseburger: Made with squid ink and bamboo charcoal, arguably a symbol of meat's destructive effect on the planet. Only available in Japan.
McDonald's Black Burger: Because the laws of competition say that once Burger King introduces a black cheeseburger, it's only a matter of time before McDonald's follows suit. You still don't have to eat it.
Domino's Specialty Chicken: It's like regular pizza, except instead of a crust, there's fried chicken. The company's marketing officer calls it "one of the most creative, innovative menu items we have ever had" -- brain power put to good use.
Arby's Meat Mountain: The viral off-menu product containing eight different types of meat that, on second read, was probably engineered by Arby's all along. Horrific, regardless.
KFC'S ZINGER DOUBLE DOWN KING: A sandwich made by adding a burger patty to the infamous chicken-instead-of-buns creation can only be described using all caps. NO BUN ALL MEAT. Only available in South Korea.
Taco Bell's Waffle Taco: It took two years for Taco Bell to develop this waffle folded in the shape of a taco, the stand-out star of its new breakfast menu.
Krispy Kreme Triple Cheeseburger: Only attendees at the San Diego County Fair were given the opportunity to taste the official version of this donut-hamburger-heart attack combo. The rest of America has reasonable odds of not dropping dead tomorrow.
Taco Bell's Quesarito: A burrito wrapped in a quesadilla inside an enigma. Quarantined to one store in Oklahoma City.
Boston Pizza's Pizza Cake: The people's choice winner of a Canadian pizza chain's contest whose real aim, we'd imagine, is to prove that there's no such thing as "too far." Currently in development.
7-Eleven's Doritos Loaded: "For something decadent and artificial by design," wrote one impassioned reviewer, "it only tasted of the latter."
Black Sabbath's Geezer Butler Arrested for Assault, Vandalism
On the Charts: Fall Out Boy Reign, While Decemberists, Manson Hit Top 10
Lil Wayne Sues Cash Money for $51 Million
Taylor Swift Trademarks 'This Sick Beat' and Other '1989' Phrases
Hear Charli XCX Sing 'Boom Clap' and 'Break the Rules' in Japanese
You Might Think You Know What This Mormon Woman Is Going To Say About Gay People, But Just Watch
Boobs, Boobies, The Girls, Tatas. Whatever You Name 'Em, They're Kinda Incredible.
They Liked Her Because She 'Talked White.' I Bet They Didn't Expect This.
90 Seconds Of Wiggling, Jiggling, Sweating, And Shaking Is All It Took To Get Me Off The Couch
Almost 50 Years Ago, They Were Sentenced To Prison For Marrying Each Other. You Read That Right.


text: Politics Obama Used To Be So Stoked About Health Care. Now…Not So Much Rough day/month/five years for the president. Dorsey Shaw BuzzFeed Staff Video available at: http://buzzfeed-video1.s3.amazonaws.com/video/2013/11/14/tiredobama-624x348.mp4. Check out more articles on BuzzFeed.com! More ▾ Facebook Conversations Next On BuzzFeed News › Witness Says Gambian Man Accused Of Homosexuality Bore... Next On BuzzFeed News › Big Mother Is Watching You: The Track-Everything Revolution... Saving... Saving... Saving... Rebuzzed! This post has been added to your Feed I know, right? And there's more where that came from. Totally! And there's plenty more to love! Broken hearted? Maybe you'll like something over here instead? I know, right? Will your friends agree? Share this Link Obama Used To Be So Stoked About Health Care. Now...Not So Much http://www.buzzfeed.com/dorsey/obama-used-to-be... Rough day/month/five years for the president. Your link was successfully shared! EDIT More Buzz Obama Administration Opens The Door To Medicare-Funded Sex Reassignment Surgery × Obama: "American Influence Is Always Stronger When We Lead By Example" × Veterans Affairs Secretary Steps Down In Wake Of Scandal × DREAMers Slam White House Over Delay To Deportation Reforms × Related Links × Note: once you save these links, they will no longer update automatically. 1. 2. 3. 4. Save Changes Cancel Facebook Conversations


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Screening for Cataracts
by Rick Alan
The purpose of screening is early diagnosis and treatment. Screening tests are usually administered to people without current symptoms, but who may be at high risk for certain diseases or conditions.
Screening Tests
A comprehensive eye examination screens for cataracts. This examination should include:
Screening Guidelines
Ask your doctor for guidelines specific to your individual situation. The American Academy of Ophthalmology recommends the following general screening guidelines for healthy adults with no risk factors for eye disease:
You should be screened more often, or as directed by your doctor, if you:
Cautionary Note: If you currently have eye symptoms, you should call your provider immediately for an evaluation. In case of an emergency, call for an ambulance immediately.
American Academy of Ophthalmology
Mayo Foundation for Medical Education and Research
The Merck Manual of Medical Information, 17th edition.
Simon and Schuster, Inc;2000.
National Eye Institute
Last reviewed October 2004 by Kimberly Rask, MD, PhD
All EBSCO Publishing proprietary, consumer health and
medical information found on this site is accredited by URAC. URAC's Health Web Site
Accreditation Program requires compliance with 53 rigorous
standards of quality and accountability, verified by
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AMSAT's new Director of Field Operations Patrick Stoddard, WD9EWK, of Scottsdale, Arizona, is hoping to return AMSAT's Field Organization to a place of prominence within the satellite organization.
"I look forward to reviving the Field Organization and re-establishing a line of communication with those who want to represent AMSAT at the local level," Stoddard said upon his appointment. "I will be reaching out to those that have assisted us in the past to ascertain their current level of interest in helping AMSAT as well as encouraging others to join our team."
Stoddard will work closely with AMSAT President Barry Baines, WD4ASW, as he begins the process of breathing new life into the Field Operations organization. As director of field operations, he will be responsible for managing AMSAT's corps of area coordinators, who represent AMSAT locally. Area coordinators serve as the "Ambassadors of AMSAT." This may include staffing AMSAT booths at hamfests, giving local/regional club presentations on AMSAT and satellite operations and serving as Elmers to those seeking information about operating through satellites.
At one time more than 100 AMSAT members were on the list of area coordinators in the US and Canada.-- AMSAT News Service http://www.amsat.org/amsat-new/news/
The ARRL Letter


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Zucchini Bread
From EatingWell.com
Not too sweet, and very fragrant, this is good bread to bake if you're trying to sell your house.
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Nutritional Information
(per serving)
Calories | 208 |
Total Fat | 6g |
Saturated Fat | 1g |
Cholesterol | 18mg |
Sodium | 233mg |
Total Carbohydrate | 37g |
Dietary Fiber | -- |
Sugars | -- |
Protein | 4g |
Calcium | 0 |
Serves: 10
Total Time: 2 hr
U.S. | Metric | Conversion chart |
- 1 cup(s) rolled oats
- 1/2 cup(s) pecan halves
- 2.25 cup(s) all-purpose flour
- 1.5 cup(s) whole-wheat pastry flour
- 1.5 tablespoon(s) baking powder
- 2 teaspoon(s) ground cinnamon
- 1/2 teaspoon(s) freshly grated nutmeg
- 1/4 teaspoon(s) ground cloves
- 1.5 teaspoon(s) salt
- 2 cup(s) packed light brown sugar
- 2 large eggs
- 2 large egg whites
- 3/4 cup(s) apple butter
- 1/3 cup(s) canola oil
- 3 cup(s) shredded zucchini, (about 2 small zucchini)
- Preheat oven to 350 degrees F. Coat two 9-by-5-inch loaf pans cooking spray. Spread oats and pecans on separate parts of a baking sheet and bake until lightly toasted, 5 to 10 minutes; let cool. Chop the pecans.
- Stir together all but 2 tablespoons of the toasted oats, the pecans, white and whole-wheat flours, baking powder, cinnamon, nutmeg, cloves and salt in a mixing bowl. Whisk together brown sugar, eggs, egg whites, apple butter and oil in another bowl; stir in zucchini. Stir the wet ingredients into the dry ingredients just until well combined.
- Divide the batter between the prepared loaf pans, smoothing the tops. Sprinkle 1 tablespoon of the reserved oats on top of each loaf. Bake until the tops feel firm when lightly pressed and a skewer inserted in the center comes out clean, 45 to 55 minutes.
- Let the loaves cool in the pans for 5 minutes; turn them out onto a wire rack to cool completely.
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Carleen, Another note. Aunt Jane's son's, Frank Kitchel's picture, was identified by an older uncle as Jr.Kitchel, so it could be very likely that Jane's husband was Frank also. This uncle has memory problems so this could be wrong. Barb Notify Administrator about this message?
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Arizona Cardinals
Former Cardinal Warner feels good about team's new coaches
Former Arizona Cardinals quarterback and current NFL Network analyst Kurt Warner experienced great success under Ken Whisenhunt.
So, it should come as no surprise that he was disappointed to see the team part ways with the only coach to lead the franchise to the Super Bowl.
A guest of Arizona Sports 620's Doug and Wolf Tuesday, Warner said "even great coaches get fired", and understands why the team made the move: instability at the quarterback situation.
So, while Warner feels like Whisenhunt will be back as a head coach real soon, he believes the Cardinals did well with the man they found to replace him.
"All I know of Coach Arians, coming into this thing, was having played against him and what he brought to the table and how he had worked with other young quarterbacks," Warner said of the head coach. He then turned his attention to assistant head coach/offense Tom Moore. "I know Tom a little bit more than I do Coach Arians."
Warner said Moore's track record is well-known, and the fact that both Arians and Moore have had success with young quarterbacks bodes well for the Cardinals.
"I had a chance to talk to Coach Arians a little bit down in New Orleans and get a sense of his excitement for this job and what he brings to the table," he added.
Most Popular
Cardinals Audio
- Brock Huard, ESPN 710 Seattle host - Friday January 30Brock Huard believes the Seahawks will beat the Patriots in Super Bowl.
- Luis Gonzalez- Former Diamondbacks outfielder - Friday January 30Luis Gonzalez reflects on the growth of the city of Phoenix.
- Bickley Blast - Friday January 30Marshawn Lynch is challenging the NFL commissioner.
- Mark Schlereth- ESPN NFL analyst - Friday January 30Mark Schlereth believes NFL players should take a stand against the owners.
- Joe Theismann- former NFL quarterback - Friday January 30Joe Theismann says Deflategate isn't a controversy at all.


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Lou My Sam Cassell Rookie so the aliens could have a picture of their leader Posted May 20, 2011 at 9:27 am | Permalink
dante Sam Cassell the alien leader!!! LOL Tooo Funny!!!!! Posted May 20, 2011 at 10:41 am | Permalink
Tony My Billy Ripken ***k-face card…..the shelter needs a comedian, right? Posted May 21, 2011 at 1:23 pm | Permalink


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A collection of news and information related to Unrest, Conflicts and War published by this site and its partners.
Top Unrest, Conflicts and War Articles
Displaying items 73-84
Building changed with changing needs
Story | May 31, 2013 | 8:29 PMHistorical records indicate the Memorial Gardens Building was used during World War II as a barracks and then possibly an infirmary, according to fairgrounds officials. "It housed fellas going through training," said Ed Miller, author of "The SAAAB... -
It's A Gray Area: It's time to take back America's moral high ground
Story | Jun 7, 2013 | 11:29 AMPresident Obama has recently renewed his plans to try to close our military prison at Guantanamo, Cuba, and has also promulgated new guidelines for our government's use of drones to assassinate people in places like Pakistan, Afghanistan and Yemen.... -
Commentary: Writing prompts reflection on 'heroes'
Story | Nov 4, 2013 | 11:37 AMAfter 10 months of writing and researching five hours a day, spilling umpteen thousand words and creating 66 chapters, I'm pages away from completing my book. I'm hoping to finish today. However, I must complete this column, teach at the Leadership... -
Carnett: Addressing a Gettysburg fascination
Story | Jul 1, 2013 | 8:38 AMWhat if? It's the age-old query of the historian. And, through its lens, one can view any war and the subsequent history. What if the Moors had won the battle of Tours? What if the Allies had been turned back at Normandy? What if Robert E. Lee had... -
Supervisor speaks up for preserving WWII building
Ralph Eugene Whitford
Fair Board approves veterans exhibit
Jewish group backs Rackauckas
Story | Feb 8, 2011 | 8:52 PMA leading Los Angeles Jewish rights organization on Tuesday came out in support of the Orange County district attorney's decision to file criminal charges against 11 students who interrupted a speech by the Israeli ambassador to the United States. In a... -
Thursday's Calendar
Friday's Calendar
Story | Feb 10, 2011 | 8:34 PMFRIDAY Chanel Spring Trunk Show Neiman Marcus at Fashion Island hosts a Chanel Spring Trunk Show, showing off its 2011 Spring Collection. For more information, call (949) 759-1900. Shabbat Service Temple Bat Yahm, 1011 Camelback St., Newport Beach,... -
Man charged with pharmacy robbery
Story | Feb 12, 2011 | 3:47 PMA Mission Viejo man is due back in court Thursday after being charged with tying up an Irvine pharmacy clerk and robbing him last week. Scott Burnett, 33, pleaded not guilty Wednesday at the Central Justice Center in Santa Ana to robbery and assault with... -
Lobdell: Here's what's pulling my heartstrings
Story | Feb 15, 2011 | 8:25 AMIt's the day after Valentine's Day, and I'm still full of red-hot passion. Here's where my heart lies: I'm in love with the proposed submarine base in Newport Harbor. It has a very James Bond vibe to it. Newport Beach yachtsman Chris Welsh (I wonder if...


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Latest STANFORD Stories
PALO ALTO, Calif., Oct. 23 /PRNewswire-FirstCall/ -- Volkswagen Group of America, Inc.
Macrophages, the scavenger cells of the body's immune system, are responsible for disposing of dying cells. Stanford University School of Medicine researchers have identified one pathway in this important process in mice that, if disrupted, causes a lupuslike autoimmune disease.
Patients reporting new low-back pain are more likely to undergo surgery if treated in an area with a higher-than-average concentration of magnetic resonance imaging machines, according to research from the Stanford University School of Medicine.
WASHINGTON, Oct. 14 /PRNewswire-USNewswire/ -- Springboard Enterprises, in partnership with Silicon Valley-based Life Sciences Angels and with the support of the Stanford Medical and Law Schools, will host a forum presenting some of the most transformational early stage ventures led by women.
Jiyai Shin captured her third title of the year Sunday, winning the LPGA Arkansas Championship in a playoff. The 21-year-old Korean star birdied the second extra hole, sinking a 10-foot putt, to defeat Sun Young Yoo and Angela Stanford after the three had finished regulation play at 9-under-par 204. Shin shot a 7-under 64, Yoo had a 68 and Stanford a 69 in the final round. The win was Shin's sixth on the tour. I still can't believe it, Shin said after her win.
Globs of human fat removed during liposuction conceal versatile cells that are more quickly and easily coaxed to become induced pluripotent stem cells, or iPS cells, than are the skin cells most often used by researchers, according to a new study from Stanford's School of Medicine.
Stanford scientists' open-source camera could change photography by giving programmers anywhere the power to change camera features and create new possibilities.
IRVINE, Calif., July 27 /PRNewswire/ -- WiSpry, Inc., the leader in programmable radio frequency (RF) semiconductor products for the wireless industry, today announced that Jeffrey Hilbert will be featured at the 7th annual AlwaysOn & STVP Summit at Stanford to be held July 28-30, 2009 in Palo Alto, Calif.
SACRAMENTO, Calif., July 23 /PRNewswire/ -- emTRAiN, Inc., the innovative leader in delivering on-demand, online HR compliance training and learning management systems, today announced that CEO and founder Janine Yancey will showcase the company's multi-media web-based corporate compliance training system at the 7th AlwaysOn & STVP Summit at Stanford.
NEW YORK, July 20 /PRNewswire/ -- Correlix, a leader in inter-party latency management for trade execution and market data in real-time, today announced that it has been selected to the 2009 AlwaysOn Global 250 List.
Latest STANFORD Reference Libraries
Symbol: VEST4 Group: Lichen Family: Verrucariaceae Growth Habit: Lichenous Native Status: NA N Classification: Kingdom Fungi – Fungi Division Ascomycota – Sac fungi Class Ascomycetes Order Verrucariales Family Verrucariaceae Genus Verrucaria Schrad. – wart lichen Species Verrucaria stanfordii Herre – Stanford's wart lichen
Symbol: TOST2 Group: Moss Family: Pottiaceae Growth Habit: Nonvascular Native Status: NA N Synonyms: HYST6 Hyophila stanfordensis (Steere) A.J.E. Sm. & H. Whitehouse Classification: Kingdom Plantae – Plants Division Bryophyta – Mosses Subdivision Musci Class Bryopsida – True mosses Subclass Bryidae Order Pottiales Family Pottiaceae Genus ...
Symbol: POPE2 Group: Dicot Family: Polygonaceae Duration: Annual Growth Habit: Forb/herb Native Status: L48 N AK N CAN N Synonyms: PEBI4 Persicaria bicornis (Raf.) Nieuwl. PELO7 Persicaria longistyla (Small) Small PEMI9 Persicaria mississippiensis (Stanford) Small PEPE19 Persicaria pensylvanica (L.) Small PEPED Persicaria pensylvanica (L.) Small var. dura (Stanford) C.F. Reed POBI11...
Symbol: POHY2 Group: Dicot Family: Polygonaceae Duration: Perennial Growth Habit: Forb/herb Native Status: L48 N AK I PR N CAN N Synonyms: PEHY7 Persicaria hydropiperoides (Michx.) Small PEHYB Persicaria hydropiperoides (Michx.) Small var. breviciliata (Fernald) C.F. Reed PEHYE Persicaria hydropiperoides (Michx.) Small var. euronotora (Fernald) C.F. Reed PEHYO Persicaria hydropiperoides...
Symbol: PHHER2 Group: Dicot Family: Solanaceae Duration: Perennial Growth Habit: Forb/herb Native Status: L48 N Distribution: PhysalisheterophyllaNees var.rowelliiStanford distribution:USA(TX) Classification: Kingdom Plantae – Plants Subkingdom Tracheobionta – Vascular plants Superdivision Spermatophyta – Seed plants Division Magnoliophyta – Flowering plants Class Magnoliopsida...
- An imitative word; an onomatopoetic word.


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My husband and I have been staying here for very long time. It was our yearly get away with our children who are now adults. More fun was added as we now take our gran kids along. Smaller resort, ocean side, wonderful pool plus Kiddie pool. Delicious reasonable meals. Beautiful large condo's. Staff is like family. You are missing out if you pass this one up.
- Reservation Options:
- TripAdvisor is proud to partner with Expedia, Hotels.com, Booking.com, Orbitz, Travelocity, Odigeo and Tingo so you can book your Torres Mazatlan Resort reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Torres Mazatlan Hotel Mazatlan


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This entry has been rejected due to incompleteness or lack of notability.
To dispute this DEADPOOL flagging, please provide suggestions for how this entry can be improved, or request editorship to help maintain this entry.
This came from a girl who thought she was a good singer. She posts videos that have had over 20million views. Here is her youtube page.
She tells brave stories such as her wisdom teeth story She also has a singing voice that is perfect for everything she sings like Baby by Justin Bieber


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C Spire makes staff promotions
by MBJ Staff
Published: May 16,2013
Tags: C Spire Wireless, Cody Mahaffey, Donna Morgan, Frank York, telecommunications
C Spire Wireless has made numerous staff news. These include Frank "Beau" York, manager, brand programs; Cody Mahaffey, manager, financial planning and analysis; Donna Morgan, manager, brand products; Amanda Long, market manager, retail, Philadelphia; John Hegi, manager, network accounting; Tammy Taylor, client account executive, Central Mississippi; Gary Dodson, client account executive, Central Mississippi; J.C. Simmons, client account executive, Central Mississippi; Nick Trentmen, client account executive; Rachel Halford, client account executive; and, Paige Griffin, client account executive. York has worked in a variety of management and non-management positions, including product specialist, marketing manager and sales representative since joining C Spire in 2006. A native of Jackson, York is a Mississippi College graduate with a bachelor's degree in marketing. Long brings over 13 years of experience to her new position. She previously served as a floating market manager and as a technology professional in the company's Starkville market. Long spent 10 years working in a variety of sales positions for Alltel Wireless before joining C Spire. A graduate of East Central Community College and Mississippi State University with a degree in marketing, Long worked 10 years in a variety of sales jobs for Alltel Wireless before joining C Spire. A native of Louisville, Long is a member of the Community Development Partnership in Philadelphia. Hegi, a certified public accountant, previously worked as a senior auditor for Horne LLP, a Ridgeland-based tax and accounting firm, for five years before joining C Spire in 2011. He previously served as manager of financial analysis and planning. A native of Madison, Hegi is a University of Mississippi graduate with a bachelor's degree in accounting. Dodson previously served as a data and IP account executive and a major accounts manager for AT&T and a senior account executive for Time Warner Telecom. A native of Hialeah, Fla., Dodson is a graduate of the University of Alabama at Birmingham with a bachelor's degree. He has received certification in Cisco Sales essentials and Burton sales skills. Simmons previously served as a property and casualty agent for Twitty Insurance Agency Inc. A native of New Albany, Simmons is a graduate of Mississippi State University with a bachelor's degree in risk management, insurance and financial and real estate-mortgage financing. He is a volunteer with the Boy Scouts of America and is a member of the MSU Alumni Board committee. Since joining C Spire in 2008, Mahaffey has worked as a financial analyst. A native of Jonesboro, La., Mahaffey is a Millsaps College graduate with a master's degree in business administration. He is a member of the Beta Gama Business Honor Society and the Financial Management Association. Morgan has over 17 years of telecommunications experience, including 13 years in a variety of management roles at Chicago-based U.S. Cellular. Prior to her latest job, Morgan was manager of C Spire's Advance Pay program. A native of Miami, Fla.,, Morgan graduated from DeVry University in Chicago with a bachelor's degree in technical management and networking. Taylor previously served as a business account executive for Comcast Cable in Jackson. Before that, she was a business account executive for Xfone Inc. A native of Jackson, Taylor is participating in Belhaven University's Aspire program and working towards a bachelor's degree in business management. She is a member of Woman in the Channel. Griffin previously served as a telecommunications specialist for St. Jude Children's Research Hospital, one of the world's leading centers for pediatric cancer treatment. A Memphis native, Griffin is a University of Memphis graduate. Trentman previously served as a business account executive for Comcast Business Services and as a regional account manager for IVR Controls, a Memphis-based software company that develops, distributes and deploys intelligent video security and surveillance platforms. A Memphis native, Trentman graduated from the University of Memphis with a bachelor's degree in sports marketing and management and volunteers as the head coach of an eighth-grade boys basketball team at St. Georges Independent School in Collierville, Tenn. A Memphis native, Halford previously served as an office equipment sales professional for R.J. Young.
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Historical Society gets a glimpse of Villa Am Meer
Date: March 13, 2013
by: Robin Hartill | City Editor
Joy Baker isn't the only one intrigued by the little house by the sea.
Approximately 90 people attended her presentation about Villa Am Meer, which means "Villa By the Sea" in German, Thursday, March 7, at the last Longboat Key Historical Society meeting of the season.
Baker, a Minnesota resident, began researching and blogging about the property in March 2010, when she saw it surrounded by construction equipment and worried it would be torn down.
The equipment was actually being used for construction of two groins at the Islander Club next door.
"I could just tell a lot of pride went into the house," she said, describing the elaborate stained-glass windows, ceramic tiles and spiral iron staircase leading to a rooftop patio, where she imagined parties occurred.
German immigrant Dr. Hermann Kohl, and his wife, Hertha, built the home in 1935. He had founded Norda Inc., in 1924, to create flavorings and additives for the beverage industry.
He had faced bootlegging charges, but about a year before he built Villa Am Meer, Prohibition ended, and the charges were dropped.
Kohl would go on to invest $15,000 for a 49% stake in what would become Tropicana Products Inc. in the 1940s.
In the audience at Baker's presentation were Hertha Kohl's great-niece, Monika Wehofsich and her daughter, Katja, who were visiting from Germany.
Wehofsich never met Hertha Kohl and knew her only from the letters they exchanged when she was a child. She discovered Baker's blog when she did an Internet search for "Dr. Hermann Kohl USA," seeking more information about her family's history.
The Kohls were childless but informally adopted their gardener's daughter, Elena Amaducci, who later married into the Benedict family and inherited the estate.
The Benedict family sold the property for $18 million in 2006.
Court records show that current owner BBC Key LLC became the owner of the property in 2009, after it went into foreclosure.
Baker doesn't know what the future of the property will be, but her research about the property, along with historic photos, are now recorded with the Florida Master Site File, a state-maintained inventory and archive, so that no matter what happens to the home, there will be a permanent record of its story.
For the full story of Villa Am Meer, visit Baker's blog, joybaker.com, and click on the "Villa Am Meer" tab.
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- Feb
8 Sarasota Music Half Marathon
7:00 am - 6:00 am - Feb
12 Longboat Key Chamber of Commerce Networking Luncheon
11:30 am - 1:30 pm - Feb
14 New Exhibit "Oh Baby" Life Cycles of the Seas
10:00 am - 5:00 pm - Feb
17 Hospitabull Evening 'A Taste of Israel'
6:00 pm
Gauls celebrate sweet 60
On Jan. 29, Jack and Joan Gaul will celebrate their 60th anniversary. -
Dora Walters goes by the book for readers
You saw her byline countless times during her 26 years at the Longboat Observer, but now, retired Senior Editor Dora Walters' byline appears on her new book, "Rewind…Playback: A Collection of Life's 'Little' Memories." -
Encore opening celebration
Members of the Longboat Key Chamber of Commerce gathered for a ribbon-cutting event Jan. 20, at Amore by Andrea.


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This guy sure knows how to send a sweet text
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25 Comments | Add Yours
by ladyinlike on 4/16/2012 4:57:03 PM
I was shocked when my parents told me we were adopting a 5 month old little boy. Today he is 4 and I couldnt imagine my ife without him!!!
by sgirl26 on 3/12/2012 7:41:14 PM
I rely want an older brother. Being any only child is rely boring and it can get boring sometimes
by emmakn99 on 2/8/2012 11:43:32 PM
Hey chica, i understand how wierd this is for you. You're uncomftorable with such a HUGE change. You sound like a great girl. You can get through this. If my parents told me they might adopt, I'd be freaking out. But, think about it this way. You're parents are willing to take another child into their lives to feed, clothe, and house. Alll of this, but the greatest thing they'll be doing is making another kid's life better. If they're willing to do that, you should to. Tell your heart the truth. You can get do it!
by DivaChica on 12/4/2011 6:22:30 PM
hey i would love to have an adopted bro or sis. younger or older i need someone new!
by nyomi on 11/15/2011 5:26:52 PM
by icebreaker373 on 11/9/2011 8:23:36 PM
by highschool1 on 10/21/2011 10:21:07 PM
by Dee_Has_Cookiez on 10/21/2011 9:09:44 PM
by kaboodle on 10/3/2011 4:11:55 PM
by npbrat29 on 9/19/2011 7:33:45 PM
by whitedress26 on 9/17/2011 4:22:50 PM
by kmbarrette on 9/17/2011 10:03:13 AM
MOD MOD MOD i think my older sis is anorexic.and im also angry because shes always been thin, even wen she did eat, and ive always been the bigger one, the uglier one, the one who doesnt get any guys, and it seems as if im more confident than HER, and theres been countless times ive cried myself to sleep becuz of tht, but i wood NEVER become anerexic just cuz ive gained a lil weight, also, my sis has (or had) depression, n wen she was 13 or 14 she started cutting, and i think she stopped, and now shes 16, shes gorgeous, shes thin, she has big boobs, and a hot bf, and SHES anorexic, im just so angry, sad, confused, worried, idk to break down and cry or scream in her face and knock her out, what am i supposed to do?!?!?
Hey girly! It sounds like you have a really good head on your shoulders, which I'm very glad to hear. As for your sister, though, you definitely need to talk an adult about this and let them know what's going on with her. Try talking to your parents about it. Show them the site and let them know how worried you are. If you can't talk to them, I'd talk to an aunt or uncle, a grandparents, or even a friend's parent that you trust. Also, guidance counselors at school would know what to do. The bottom line is, your sister needs help before this gets any worse.
by guitargirl7321 on 9/11/2011 6:22:35 PM
by smileysevvie on 9/8/2011 10:11:32 PM
Hey girlies! Please visit my profile and leave a comment if you want advice on anything! I can give advice on guys, friends, mean girls, popularity, sports, growing up, middle school, tough stuff, beauty, fitness....practically anything! Just leave a comment (i think I said that) and I will answer it as soon as I can.
by basketballcutie11 on 9/4/2011 7:38:05 AM
wow, that something interesting... i never thought about that... wow! if you ladies need any advice comment on my profile!(:
by purpleorchid on 9/4/2011 12:18:45 AM
Hey, girlie. I totally know how you're feeling right now. My family did foster care for about four years before we adopted my eight-year-old sister. I'm not going to lie, it was hard getting used to having another sibling besides my brother, and having to share my parents attention between 3 people, but it got easier. So whatever your parents decide, hang in there. It will all work out.
by hippiechick96 on 9/3/2011 10:07:43 PM
A lil wierd I think. I didn't like adoption when my step mom did it. Hey Girlies, Come check out some of these awesome clubs! tween chat club,Club Tough Stuff, Lost Without Ur Bestie. You might want to checkout this cool blogging site i you like blogging, it's new so don't dis on it: kdblogger.weebly.com
by HaloAngel99 on 9/1/2011 3:52:45 PM
in my Pretty Little Liars fan club, you can get the scoop, take quizzes, and talk about the books. we have over 120 members and growing! all you have to do is click on my username,view my clubs, and click on PLL fan club. its that easy! why not join?
by cutiecayley333 on 9/1/2011 2:57:05 PM
Join ramdom rocks (random with a m)
by Tbarb14 on 9/1/2011 1:07:46 PM
by shelly_rocks on 8/31/2011 6:46:03 PM
My mom said that once. But she said that she's adopting a child once we all leave the house.
by NJessicaN on 8/31/2011 6:38:42 PM
weird coincidence, my friend was just talking about it with me today! her family might adopt a little girl! Hey girls! Whether you're obsessed with makeup or puzzled about fashion, this is the club for you. My club Beauty Queens is the best place for fashion and beauty advice and tips! You can talk about anything from clothing and makeup to health and self-confidence, because inner beauty is important too! I also post beauty quotes, surveys, and tutorials, and I'm open to suggestions! Also check out beautiful babes bootcamp- a club devoted to making you look great inside and outside!!! It's loaded with great tips about fitness, health, fashion, and makeup. Check it out today! <3 <3 <3 thecupcakequeen
by thecupcakequeen on 8/31/2011 6:10:04 PM
I have 3 little brothers, and one older sister, and two of my little brothers are adopted from Ukraine. Their names are Max, and JT and they both have Down Syndrome. They've been home since Christmas 2009 (Almost 3 years now!!) and they are sooooo amazing! In some countries, children with special needs get put into an adult mental institution at the age of four if they have not been adopted, and die within a year from lack of food, water, and exercise. It's really heartbreaking!! But, that's not my point here. My point is, adoption is a great thing. It's giving a child a life outside of an orphange, or foster home. I think you are worried about change in your life? Change can be a great thing. Especially in adoption. My favorite quote about adoption- "Adopting one child won't change the world, but for that child, the world will change." Try to be open minded. It'll be just like your mom having a baby, except the "baby" will probably be a little older.
by holyhotness13 on 8/31/2011 4:27:01 PM
oh i dont have to worry about something like this my family has 7 kids and 2 grandchildren (from my older sister who is 21 and married) i think were good for now
by krazykotabug on 8/31/2011 4:17:15 PM
Get ready for V-Day with our "Things We Heart" giveaway!
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News 1 - 10 of 10
Tony Romo Shares First Picture of His Baby Boy
April 12, 2012 11:04:20Along with the release of the family photo, words come out that the Dallas Cowboys quarterback and wife Candice Crawford have taken their bundle of joy home from the hospital.
Tony Romo Has Become Proud Father to Baby Boy
April 11, 2012 10:04:39The first child of the Dallas Cowboys quarterback and wife Candice Crawford was born Monday, April 9 and named Hawkins Crawford Romo, a spokesperson confirms.
Tony Romo Marries Candice Crawford in Texas, the Details
May 30, 2011 12:05:13The couple picked Todd Fiscus as the wedding planner, white as the theme, 'man food' as the menu and Cadillac as their vehicle.
Tony Romo to Tie the Knot at Historic Mansion
May 04, 2011 04:05:33Words are, the Dallas Cowboys quarterback will exchange wedding vows with Chace Crawford's sister, Candice, at the Arlington Hall in late May.
Pic of Tony Romo Proposing to Candice Crawford Surfaces
December 20, 2010 02:12:45The football player, who is sidelined for collarbone injury this month, went traditional in proposing to the news anchor, kneeling in front of her and showing her the huge rock.
Tony Romo Engaged to Candice Crawford, Pic of Ring Revealed
December 17, 2010 03:12:59While Romo chose to dump Jessica Simpson on her birthday, he decided to take his relationship with Candice Crawford to the next level on her birthday.
'Planet of the Apes' Prequel to Be Directed by Rupert Wyatt
March 12, 2010 03:03:23The 'The Escapist' helmer allegedly has been hired to replace Scott Frank, who was previously attached to helm this upcoming project about the origins of how the Apes take over Earth.
Jessica Simpson: Love Tony Romo's Butt, Hate John Mayer's Racy Comments
March 12, 2010 03:03:16In an interview with David Letterman, the 32-year-old beauty shares about her relationships with Tony Romo, whose butt she loves to watch, and John Mayer, whose raunchy comments disappoint her.
Chris Jericho Nearly Stays in Jail After Being Late Arriving at Court
February 17, 2010 02:02:38Chris Jericho and his fellow wrestler almost stay behind bars after they are late attending the court although in the end they settle the case by pleading guilty to the count and paying a $159 fine.
Jessica Simpson: I Still Regularly Talk to Tony Romo
February 17, 2010 02:02:53Although being dumped by the Dallas Cowboys star on her birthday, Jessica Simpson claims that there is no hard feelings towards him as they still keep in touch post their break-up.


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Publications (16)41.21 Total impact
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ABSTRACT: The Nile tilapia is a cultured teleost fish in which males grow faster and larger than females, and mono-sex culturing could avoid unwanted reproduction during grow-out. Sex control in tilapia is an important issue in aquaculture. In the present study, randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) fingerprinting were used to screen pooled and individual DNA samples from XX, XY, and YY fish for sex-linked markers. Four sex-linked markers (Marker-1 from RAPD and Markers-2, -3, and -4 from AFLP) were obtained and mapped to LG23, a small chromosome, by sequence analysis and fluorescence in situ hybridization (FISH). In addition, a total of 32 pairs of primers were designed based on the sequences of scaffolds 7, 29, and 101 on LG23, and these were used to screen genetic differences between X and Y by PCR. One of these (Marker-5) produced a detectable difference between XX, XY, and YY individuals. Eight pairs of primers based on the sequence characterized amplified region (SCAR) were designed and successfully converted to SCAR markers, which were used to sex progeny from different crosses of known genotypes for validation. Subsequently, Markers-4 and -5 were used to sex eight populations of Nile tilapia collected from different fish farms in China, which gave concordance rates that ranged from 76 to 100%. Based on the recombination rate derived from the progeny of XX (♀) × XY (♂), Markers-1, -2, -3, -4, and -5 were estimated to be around 5, 3, 5, 2 and 0 cM away from the sex-determining locus (SD), respectively. Marker-5, which mapped close to the SD reported previously (Eshel et al., 2012), was used for selective breeding of genetic male tilapia (GMT).Aquaculture 09/2014; 433:19–27. · 1.83 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Feminization of animals derived from areas polluted by endocrine disrupting chemicals (EDCs) has been observed in all classes of vertebrates. However, feminization of artificially reared offspring by feeding of specific living organisms has never been reported.Environmental research. 06/2014; - [Show abstract] [Hide abstract]
ABSTRACT: Females with differentiated ovary of a gonochoristic fish, Nile tilapia, were masculinized by long term treatment with an aromatase inhibitor (Fadrozole) in the present study. The reversed gonads developed into functional testes with fertile sperm. The longer the fish experienced sex differentiation, the longer treatment time was needed for successful sex reversal. Furthermore, Fadrozole induced sex reversal, designated as secondary sex reversal (SSR), was successfully rescued by supplement of exogenous 17β-estradiol. Gonadal histology, immunohistochemistry, transcriptome and serum steroid level were analyzed during SSR. The results indicated that spermatogonia were transformed from oogonia or germline stem cell-like cells distributed in germinal epithelium; while Leydig and Sertoli cells probably came from the interstitial cells and granulosa cells of the ovarian tissue, respectively. The transdifferentiation of somatic cells, as indicated by the appearance of Dmrt1 (pre-Sertoli cells) and Cyp11b2 (pre-Leydig cells) positive cells in the ovary, provided micro-niche for the transdifferentiation of germ cells. Decrease of serum 17β-estradiol was detected earlier than increase of serum 11-ketotestosterone, indicating decrease of estrogen was the cause while increase of androgen was the consequence of SSR. The sex reversed gonad displayed more similarity in morphology and histology with a testis while the global gene expression profiles remained closer to the female control. Detailed analysis indicated that transdifferentiation was driven by suppression of female pathway genes and activation of male pathway genes. In short, SSR provides a good model for study of sex reversal in teleosts, and for understanding of sex determination and differentiation in non-mammalian vertebrates.Endocrinology 01/2014; · 4.72 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Transcription activator-like effector nucleases (TALENs) are a powerful approach for targeted genome editing and have been proved to be effective in several organisms. In this study, we reported that TALENs can induce somatic mutations in Nile tilapia, an important species for worldwide aquaculture, with reliably high efficiency. Six pairs of TALENs were constructed to target genes related to sex differentiation, including dmrt1, foxl2, cyp19a1a, gsdf, igf3 and nrob1b, and all resulted in indel mutations with maximum efficiencies of up to 81% at the targeted loci. Effects of dmrt1 and foxl2 mutation on gonadal phenotype, sex differentiation and related gene expression were analyzed by histology, immunohistochemistry and real-time PCR. In Dmrt1 deficiency testes, phenotypes of significant testicular regression, including deformed efferent duct, degenerated spermatogonia or even a complete loss of germ cells, and proliferated steroidogenic cells, were observed. In addition, disruption of Dmrt1 in XY fish resulted in increased foxl2, cyp19a1a expression, serum estradiol-17β and 11-ketotestosterone levels. On the contrary, deficiency of Foxl2 in XX fish exhibited varying degrees of oocyte degeneration, significantly decreased aromatase gene expression and serum estradiol-17β levels. Some Foxl2 deficiency fish even exhibited complete sex reversal with high expression level of Dmrt1 and Cyp11b2. Furthermore, disruption of Cyp19a1a in XX fish led to partial sex reversal with Dmrt1 and Cyp11b2 expression. Taken together, our data demonstrated that TALENs are an effective tool for targeted gene editing in tilapia genome. Foxl2 and Dmrt1 play antagonistic roles in sex differentiation in Nile tilapia via regulating cyp19a1a expression and estrogen production.Endocrinology 10/2013; · 4.72 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Fibroblast growth factors (FGFs) have been proved to participate in a wide variety of processes, including growth, differentiation, cell proliferation, migration, sex determination and sex differentiation. The roles of FGF9/16/20 subfamily members in the gonadal development of teleost fish have not yet been reported. Three FGFs (16, 20a and 20b) of the FGF9/16/20 subfamily were cloned from the Nile tilapia by RT-PCR and RACE. Phylogenetic, bioinformatic and syntenic analyses demonstrated that these cloned FGFs are genuine FGF16, 20a and 20b. Our analyses further supported the non-existence of FGF9 ortholog and the existence of two FGF20 paralogs in teleost genomes. Tissue distribution analysis by RT-PCR demonstrated that FGF16 was expressed in a wide range of tissues including the testis and ovary, FGF20b in the brain, pituitary, intestine and ovary, but not in the testis, while FGF20a in the brain, pituitary and spleen, but not in the gonad. These results were consistent with the Northern blot analysis. The expression profiles of FGF16 and FGF20b during normal and sex reversed gonadal development were investigated by real-time PCR. Both showed much higher expression in the XX ovary and 17 beta-estradiol induced XY ovary compared with the XY testis and fadrozole and tamoxifen induced XX testis, with the highest in both sexes at 120 dah. Strong signals of FGF16 and FGF20b were detected in phase II oocytes by in situ hybridization. These data suggest that FGF9/16/20 subfamily is involved in the early oocyte development of the female.Fish Physiology and Biochemistry 03/2012; 38(5):1427-39. · 1.68 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: P450 11beta-hydroxylase, encoded by P450(11beta) gene, is a key mitochondrial enzyme to produce 11beta-hydroxy testosterone, substrate for the production of 11-ketotestosterone (11-KT), which has been shown to be potent androgen in several fish species. In the present work, two alternative splicing isoforms i.e. P450(11beta)-1 and P450(11beta)-2 cDNAs were cloned from the Nile tilapia, Oreochromis niloticus. They were 1614 and 1227bp in length with open reading frames encoding proteins of 537 and 408 amino acids, respectively. In contrast to P450(11beta)-1, which derived from 9 exons of the P450(11beta) gene, the 7th and 8th exons were absent in P450(11beta)-2. Tilapia P450(11beta)-1 shares the highest homology with that of medaka, Oryzias latipes. Expressions of P450(11beta)-1 and -2 were detected in the kidney and head kidney of both sexes, and in the testis but not in the ovary, with P450(11beta)-2 lower than P450(11beta)-1. Ontogenic expressions of both isoforms were detected in testis from 50dah onwards. P450(11beta)-1 and -2 were strongly expressed in sex reversed XX testis after fadrozole and tamoxifen treatment, but completely inhibited in 17beta-estradiol induced XY ovary. The existence of two alternatively spliced isoforms and the sexual dimorphic expression of P450(11beta)s were further confirmed by Northern blot. Strong expression signals in Leydig cells and weak signals in spermatogonia were detected by in situ hybridization and immunohistochemistry. Taken together, our data suggest a role for P450(11beta) in the spermatogenesis of tilapia through the production of 11-KT in testis, in addition to cortisol production in head kidney.General and Comparative Endocrinology 07/2009; 165(1):34-41. · 2.82 Impact Factor -
Article: Duplication and distinct expression patterns of two thrombospondin-1 isoforms in teleost fishes.
[Show abstract] [Hide abstract]
ABSTRACT: Two types of thrombospondin-1 (named TSP-1a and TSP-1b) were cloned from two species of teleosts, the Nile tilapia and medaka. Phylogenetic analysis of these TSP-1 sequences, together with those available from other vertebrates further demonstrated that two types of TSP-1 exist only in teleosts, extending the finding in fugu and tetraodon to two additional fish species. The expression of both genes was examined using tilapia at various developmental stages. Tilapia TSP-1a and TSP-1b were each expressed in a wide range of tissues examined. The early expression of TSP-1b in both XX and XY gonads from 5dah (day after hatching) onwards suggested an important role in the formation of gonads, while the expression of TSP-1a only in ovaries during later stages of development (from 120dah onwards) may suggest that TSP-1a is involved in oogenesis. During the 14-day spawning cycle, the expression of both types of TSP-1 exhibited distinct peaks at day 5 (peak of vitellogenesis) and day 12 (oocyte maturation). In situ hybridization analyses revealed differential expression, with TSP-1a occurring in granulosa cells and TSP-1b in theca cells. Furthermore, both TSP-1a and -1b were expressed in skeletal tissues but with clear temporal and spatial differences. In contrast, only TSP-1b was found in the myosepta. The positive signals of both TSP-1a and TSP-1b were also detected in the heart and spleen, and TSP-1a in brain and intestine by both RT-PCR and in situ hybridization.Gene Expression Patterns 07/2009; 9(6):436-43. · 1.36 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Distinct from the conventional IGFs (IGF-1 and IGF-2), here we report the identification of a novel IGF (herein called IGF-3) encoded by a separate gene from teleost species. The IGF-3 cDNA sequences were cloned from tilapia and zebrafish, and predicted from the medaka genome and EST databases. Similar to IGF-1 and IGF-2, IGF-3 is also comprised of five domains (B, C, A, D, E) with a similar tertiary protein structure, despite a low sequence homology among them. Phylogenetic analysis reveals that the IGF-3 sequences from different teleosts cluster to form a distinctive clade separate from IGF-1 and IGF-2. The expression of this novel IGF-3 is gonad-specific and starts early in fish development. In situ hybridization revealed that IGF-3 is expressed in the somatic cells and later in granulosa cells of the ovary, and in the interstitial cells of the testis. These findings highlight the importance of this novel IGF in teleost gonadal development and reproduction.Biochemical and Biophysical Research Communications 04/2008; 367(2):336-41. · 2.28 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The Nile tilapia, a gonochoristic teleost fish with an XX/XY sex-determining system, provides an excellent model for studying gonadal sex differentiation because genetic all-females and all-males are available. In this study, we used quantitative real-time RT-PCR to determine the precise timing of the gonadal expression of 17 genes thought to be associated with gonadal sex differentiation in vertebrates. Gonads were isolated from all-female and all-male tilapia before (5-15 days after hatching [dah]) and after (25-70 dah) morphological sex differentiation. The transcript of aromatase (cyp19a1a), an enzyme responsible for producing estradiol-17beta, was expressed only in XX gonads at 5 dah, with a marked elevation in expression thereafter. In contrast, mRNA expression of steroid 11beta-hydroxylase (cyp11b2), an enzyme responsible for the synthesis of 11-ketotestosterone (11-KT, a potent androgen in fish), was found in XY gonads from 35 dah only. These results, combined with the presence of transcripts for other steroidogenic enzymes and estrogen receptors in XX gonads at 5-7 dah, are consistent with our earlier suggestion that estradiol-17beta plays a critical role in ovarian differentiation in tilapia, whereas a role for 11-KT in testicular differentiation is questionable. A close relationship between the expression of foxl2, but not nr5a1 (Ad4BP/SF-1), and that of cyp19a1a in XX gonads suggests an important role for Foxl2 in the transcriptional regulation of cyp19a1a. Dmrt1 exhibited a male-specific expression in XY gonads from 6 dah onward, suggesting an important role for Dmrt1 in testicular differentiation. Sox9 and amh (anti-Mullerian hormone) showed a testis-specific expression, being evident only in the later stages of testicular differentiation. It is concluded that the sex-specific expression of foxl2 and cyp19a1a in XX gonads and dmrt1 in XY gonads during early gonadal differentiation (5-6 dah) is critical for undifferentiated gonads to differentiate into either the ovary or testis in the Nile tilapia.Biology of Reproduction 03/2008; 78(2):333-41. · 3.45 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Two isoforms of Vasa cDNA, derived from the 5′alternative splicing of the same gene, were isolated and characterized in Southern catfish, Silurus meridionalis, by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Analysis of the nucleotide sequences revealed that the full length cDNA of Southern catfish Vasa (scVasa) comprises 2525 base pairs (bp) with an open reading frame (ORF) of 1989 bp, encoding 662 amino acids, while that of Vasa short form (scVasa-s) comprises 2438 bp with an ORF of 1926 bp, encoding 641 amino acids. Except the difference in 5′-untranslated region, scVasa-s also lacks a part of the 5′-ORF region found in the scVasa. Both of the two deduced amino acid sequences contain all the eight conserved motifs characteristic to the DEAD-box protein family and four arginine-glycine-glycine motifs unique to Vasa proteins. scVasa showed the highest similarity to Vasa homolog of giebel carp (73.3%). Tissue distribution analysis by RT-PCR revealed that these two isoforms were exclusively expressed in the gonads of both sexes. In adult fish, scVasa was found to be mainly expressed in the primary oocytes at phase Ⅰ and Ⅱ in the ovary while in the spermatogonia and primary spermatocytes in the testis by in situ hybridization. Semi-quantitative RT-PCR analysis showed that the expressions of both scVasa isoforms were much higher in ovarian recrudescent stage (mainly with phase Ⅱ oocytes) than in vitellogenic stage (mainly with phase Ⅲ and Ⅳ oocytes) [Acta Zoologica Sinica 54(6):1051–1060, 2008].Acta Zoologica Sinica. 01/2008; - [Show abstract] [Hide abstract]
ABSTRACT: Recently we reported the isolation of two types of cytochrome P450c17s (steroid 17alpha-hydroxylase/C17, 20 lyase) encoded by two different genes, from genomes of teleost fish. In this study, we characterized the expression profile, enzymatic activity and transcriptional regulation of P450c17-I and -II in the medaka ovary. Similar to tilapia, medaka P450c17-I possessed both hydroxylase and lyase activities, while P450c17-II possessed only the hydroxylase activity. In situ hybridization and gene expression profiles during 48h prior to spawning indicated that P450c17-I is responsible for the production of estradiol-17beta during oocyte growth, while P450c17-II for the production of 17alpha, 20beta-dihydroxy-4-pregnen-3-one during oocyte maturation and cortisol production in the head kidney. Luciferase assays and expression profiles of transcription factors as revealed by real time PCR suggested that P450c17-I and -II expression are tightly controlled by Ad4BP/SF-1, Lrh-1, Foxl2, and Dax1 during the 48 h prior to spawning.Biochemical and Biophysical Research Communications 11/2007; 362(3):619-25. · 2.28 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Dax1 is a member of an unusual orphan nuclear receptor family, and is known to regulate P450arom in mammals and is involved in sex differentiation in some vertebrates. To investigate whether Dax1 is involved in the regulation of the steroidogenic pathway for estrogen biosynthesis in medaka ovarian follicles, we isolated Dax1 cDNA from adult medaka ovaries and analyzed its expression pattern in medaka gonads. In adult ovaries, Dax1 mRNA was detected only in postvitellogenic follicles and was not detected in previtellogenic and vitellogenic follicles. In adult testis, Dax1 mRNA was not detected. We compared the expression pattern of Dax1 with that of Foxl2, Ad4BP/Sf-1, P450c17, and P450arom by in situ hybridization using adjacent sections. In contrast to Dax1 expression, these genes were co-expressed in vitellogenic follicles but were not detected in postvitellogenic follicles. Thus, in medaka ovarian follicles, Dax1 did not show any overlapping expression patterns against Foxl2, Ad4BP/Sf-1, P450c17, and P450arom. Moreover, co-transfection experiments demonstrated that Dax1 inhibits Ad4BP/Sf-1- and Foxl2-mediated P450arom expression. On the other hand, during early sex differentiation, Dax1 mRNA was not detected in both males and females. Our results suggest that Dax1 down-regulates Ad4BP/Sf-1- and Foxl2-mediated P450arom expression in medaka ovarian follicles.Molecular Reproduction and Development 11/2007; 74(10):1239-46. · 2.68 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Cytochrome P450c17 is the single enzyme that mediates the 17 alpha-hydroxylase and 17, 20 lyase activities during the biosynthesis of steroid hormones in the gonads and adrenal gland. However, the mechanism underlying its dual action continues to be a controversy in the field of steroidogenesis in fish. In an attempt to resolve this issue, we identified a novel type of P450c17 (P450c17-II) by an in silico analysis from the genomes of six fish species. We cloned P450c17-II from tilapia and medaka, and comparison with the conventional P450c17-I revealed that they differ in gene structure and enzymatic activity. Enzymatic assays by thin-layer chromatography revealed that P450c17-II possesses only the 17 alpha-hydroxylase activity without any 17, 20 lyase activity, unlike P450c17-I, which has both these activities. In testis, both P450c17-I and -II express in the interstitial cells. Remarkable differences, revealed by in situ hybridization, in the expression patterns of the P450c17-I and -II in the ovary and head kidney of tilapia during various stages of development strongly suggest that P450c17-I is responsible for the synthesis of estradiol-17beta in the ovary, whereas P450c17-II is required for the production of C21 steroids such as cortisol in the head kidney. More interestingly, a temporally controlled switching is observable in the expression of these two genes during the steroidogenic shift from estradiol-17beta to the C21 steroid, 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (maturation-inducing hormone of fish oocytes) in the fish ovary, revealing a role for P450c17-II in the production of hormones that induce oocyte maturation in fish.Endocrinology 10/2007; 148(9):4282-91. · 4.64 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Increasing evidence suggests the crucial role of estrogen in ovarian differentiation of nonmammalian vertebrates including fish. The present study has investigated the plausible role of Foxl2 in ovarian differentiation through transcriptional regulation of aromatase gene, using monosex fry of tilapia. Foxl2 expression is sexually dimorphic, like Cyp19a1, colocalizing with Cyp19a1 and Ad4BP/SF-1 in the stromal cells and interstitial cells in gonads of normal XX and sex-reversed XY fish, before the occurrence of morphological sex differentiation. Under in vitro conditions, Foxl2 binds to the sequence ACAAATA in the promoter region of the Cyp19a1 gene directly through its forkhead domain and activates the transcription of Cyp19a1 with its C terminus. Foxl2 can also interact through the forkhead domain with the ligand-binding domain of Ad4BP/SF-1 to form a heterodimer and enhance the Ad4BP/SF-1 mediated Cyp19a1 transcription. Disruption of endogenous Foxl2 in XX tilapia by overexpression of its dominant negative mutant (M3) induces varying degrees of testicular development with occasional sex reversal from ovary to testis. Such fish display reduced expression of Cyp19a1 as well as a drop in the serum levels of 17beta-estradiol and 11-ketotestosterone. Although the XY fish with wild-type tilapia Foxl2 (tFoxl2) overexpression never exhibited a complete sex reversal, there were significant structural changes, such as tissue degeneration, somatic cell proliferation, and induction of aromatase, with increased serum levels of 17beta-estradiol and 11-ketotestosterone. Altogether, these results suggest that Foxl2 plays a decisive role in the ovarian differentiation of the Nile tilapia by regulating aromatase expression and possibly the entire steroidogenic pathway.Molecular Endocrinology 04/2007; 21(3):712-25. · 4.20 Impact Factor -
Article: Molecular cloning and analysis of gonadal expression of Foxl2 in the medaka, Oryzias latipes.
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ABSTRACT: Foxl2 is a member of the winged helix/forkhead family of transcription factors and is known to be involved in ovarian development in some vertebrates. To address the role of Foxl2 in ovarian differentiation in medaka, we isolated Foxl2 cDNA and analyzed its expression patterns during sex differentiation. Expression of Foxl2 started in somatic cells surrounding germ cells in XX gonads, just after initiation of ovarian differentiation, and was maintained in granulosa cells throughout ovarian development. In the adult ovary, Foxl2 was expressed in previtellogenic and vitellogenic follicles, but expression ceased in postvitellogenic follicles. In contrast, Foxl2 mRNA could not be detected in testes. In addition, Foxl2 and aromatase mRNAs were co-localized in some somatic cells located on the ventral side of developing XX gonads. Our results suggested that Foxl2 was not involved in ovarian determination, but was involved in differentiation of granulosa cells in medaka.Biochemical and Biophysical Research Communications 06/2006; 344(1):353-61. · 2.28 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Piscine DAX1 and SHP cDNAs with an open reading frame encoding 296 and 258 amino acid residues, respectively, as well as SHP partial gene fragment, were cloned from Nile tilapia. Phylogenetic analyses of DAX1s, SHPs, and homologous EST fragments indicate that DAX1 and SHP are conserved in gene structure and are present throughout vertebrates. A single band of approximately 1.4kb for DAX1 and of approximately 1.2kb for SHP was detected in the Northern blot analysis. Tissue distribution analysis by RT-PCR showed that fish DAX1 and SHP mRNAs are widely expressed in adult tissues, with the most abundant expression in gonads and liver, respectively. DAX1 and SHP were also detected in gonads of both sexes at 5-90 days after hatching (dah). However, the expression of DAX1 is weak at 5 and 10dah and then significantly up-regulated between 10 and 15dah, whereas the expression of SHP is moderate and consistent during the ontogeny.Biochemical and Biophysical Research Communications 10/2002; 297(3):632-40. · 2.28 Impact Factor
Publication Stats
490 | Citations | |
41.21 | Total Impact Points | |
Top Journals
Southwest University in Chongqing
Pehpei, Chongqing Shi, China
National Institute for Basic Biology
Okazaki, Aichi, Japan


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BVDA America Silmark with Paste Hardener, Low Viscosity, 5.3 Ounce in Tube, Black
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The BVDA America Silmark (Black) is a low viscosity casting compound can be mixed with hardener and poured over the print developed with fingerprint powder. After the silicone rubber has cured, the fingerprint is lifted. Depending on the color of the fingerprint powder that was used, white or black silicone rubber is used for contrast. The white rubber has been formulated in such a way that backlighting of the cast is still possible.
Viscosity | Low Viscosity |
Weight | 5.3 Oz/150.25g |


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Item Description
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100% cotton preshrunk jersey. HD fabric provides a superior printing canvas. Double-needle coverstitched front neck. Shoulder-to-shoulder tape. Double-needle stitched bottom hem. Ribbed cuffs with concealed seam. One-piece seamed 1x1 ribbed collar; CPSIA Compliant.
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