text:
In fact, King was a radical. He believed that America needed a "radical redistribution of economic and political power." He challenged America's class system and its racial caste system.
Read Whole Story
As the lineup is shaping up, it looks like it could be similar to previous elections: There will be a long list of equally unappealing candidates. Some dull person will be selected, having little chance to win against any potential Democratic candidate.
The Electoral College, gerrymandering, photo IDs and other voter suppression tactics are just some of the practices that keep America from being a democracy.
This story was originally posted at Jacobin, a print quarterly that offers socialist perspectives on politics and economics. Support Jacobin and buy a...
I propose that we abandon using the term "conservative" and replace it with the term "capitalist" in the meaning of a supporter of capitalism. Similarly, I suggest that we abandon using the term "liberal" or "progressive" and replace it with "socialist" as a supporter of socialism.
On November 4, 1914 Meyer London became the first Socialist on the East Coast (and only the second) to be elected to Congress.
I, too, dream America. I do not, however, believe that capitalism delivers on the promises rehearsed every November.
The Telluride Film Festival offers a great span of cinema, from premiering Oscar contenders to silent-era black-and-white gems to small budget independents that will never play widely but may earn an enduring cult audience.
There is a slogan out there of people, planet and profit. It is on this point that the global society is slowly reaching a boiling point where being exploited by the business community is unbearable.
The next time you read a controversial opinion article, instead of talking about "lynching" the author or what "gauge" shotgun you're going to use when you shoot him, maybe present a better idea to solve the problems he's trying to address.
Perhaps socialism in America is just a little more complicated than which party the state voted for.
Socialism embraced some liberal goals but wanted to offer the security of the old order where disturbing social change was minimized and what change was allowed was carefully controlled.
Until the moment the plane took off, we feared we might be in clear and present danger. We had just spent the last month seeking refuge in a makeshift panic room we created in our Caracas apartment.
Hungary has long been divided between its liberal and cosmopolitan capital and the more conservative countryside. During the Communist era, a small de...
With all due respect to Sen. McCain, I have a different take on this. I, too, am outraged by the lack of care that many of our veterans have received, but I'm not at all bewildered by it. In fact, I saw it coming for years.
Thomas Piketty seems to have stirred up a new Red Scare. His new book, Capital in the Twenty-First Century, is being hailed as the most important b...
It's no surprise when American bishops talk about canonizing Dorothy Day as a saint, as they've done in recent years. For me, her life embodies the heart of Christianity because of the way she constantly tried to translate her faith into good works.
Some people may confuse my criticism of capitalism as reason enough to question my patriotism. These are the same people who still think the United States is a capitalistic country. It's not.
Thinking the Earth is flat was a stage in our maturation. The current form of capitalism is only a stage in our economic maturation. It is the opposite of collusion for self-interest. It is cooperation for the common good.
Take a moment, please, to note the passing of a distinguished spokesman for the left, a man both firebrand and gadfly, of whom many Americans have never even heard. Yet what he did and said are of importance to us all and especially to the cause of democracy.
In King's faith-based Democratic Socialist idea and in his thoroughly humane vision of the Beloved Community that would embody it, there indeed is 'nothing which all cannot have their counterpart of on equal terms.'
It is evident that the problems of inflation, scarcity, crime and violence are issues that affect all Venezuelans equally, regardless of their political affiliation or ideologies. Why, then, is the population still divided?
I am actively recruiting conspirators to take part in a plan to save the world. There are no clandestine meetings, no high-risk behaviors, and no financial investments required. No special equipment is needed.
Robert Reich, the perfectly-sized* former Secretary Of Labor, recently wrote about Facebook's acquisition of WhatsApp, the company that mark...
When Poland went through its 'shock therapy' years of the early 1990s, many people lost out as a result of the economic reforms. The unemployment rate went up rapidly from under one percent in January 1990 to over 16 percent in 1994.
Get top stories and blog posts emailed to me each day..


text:
A good place for a get away. The location of the hotel is very good, right on the beach and a five minute drive from the shopping and business area. Beach view was really good but the bathrooms need some serious upgrades. Corrosion on taps and our room had a bath with large cracks in it which, If you wanted to take a bath, could definitely deter you. This was the major down side.
- Official Description (provided by the hotel):
- A tropical paradise at the Sandcastle Resort at Lido Beach in Sarasota, FloridaSandcastle Resort at Lido Beach has a relaxing sunny resort flavor, with a storybook seaside setting on a 600 foot strand of private, natural white sand beach on the Gulf of Mexico.Sun. Sand. Swaying Palm Trees. And Great Service, Too. You'll be treated to thoughtful touches, such as a morning newspaper and a small refrigerator for chilling beverages and snacks. Free wifi is also available throughout the property. Plus the pleasures of outdoor dining for both breakfast and lunch are virtually right outside your door. What's more, our recreation staff can provide personalized sailing lessons. There's a convenient Gift Shop on premises and we're within easy walking distance of popular St. Armands Circle, with its fine shopping and entertainment attractions. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Expedia, Booking.com, Orbitz, Priceline, Despegar.com, getaroom.com, Hotels.com, Odigeo, TripOnline SA and Tingo so you can book your Sandcastle Resort at Lido Beach reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Helmsley Sandcastle Sarasota
- Sarasota Helmsley Sandcastle
- Helmsley Sandcastle Hotel Sarasota

subset: commoncrawl


text:
Crystal Cove State Park Moro Campground
Laguna Beach,
Phone: (949) 494-3539
Visit Campground Website
Sites: 57
Recent Rate: $35 4/2014
Latitude: 33.5612435380198
Longitude: -117.821975932847
These reviews are the opinion of an RVParkReviews' member and not the views of RVParkReviews.com
Date of Stay: April, 2014 - $35.00
I stayed in the non hookup sites with maximum RV size of 25'. The $35 rate is for the winter off season ending May 1. There is now access to campground from both northbound and southbound Pacific Coast Highway-controlled by a signal. Campground is nicely terraced with ocean views from most or all campsites. The campground is located about 150 ft. vertically up from ocean via a paved road/sidewalk-set back maybe half mile. A steep but short climb. Ocean at this location was beautiful during my stay mid April 2014 and relatively clear and blue. During my stay during spring break lots, of families and kids looked to be having fun. No behavior problems or excess noise. Restrooms very clean by CA state park standards. Sites nice sized, but some leveling required. Somewhat narrow access roads in campground. Campground is planted in native vegetation which was blooming and fragrant during my visit. No wood burning. We camped at Crystal Cove State Park Moro Campground in a Motorhome.
Date of Stay: April, 2014 - $61.00
We stayed here for one night. The park is situated on a bluff overlooking the ocean. There are numerous trails for hiking and biking. In order to get to the beach you have to walk or drive down a steep grade to the lower parking area, then walk through a tunnel to the beach. All the spots are generally level, with a gravel base. The dump station has three sites located near the ranger station, a nice feature. Restrooms are very clean, tokens required for showers, dispensers at each shower building. The closest grocery store is approximately one mile, Trader Joe's. I felt the camping rate of $61.00 was excessive and increases in May, but it's CA. We camped at Crystal Cove State Park Moro Campground in a Motorhome.
Date of Stay: March, 2014 - $55.00
As a campground, Crystal Cove is not particularly beautiful. There are distant ocean views from just about all sites, but the landscaping is poorly-planned and kind of ugly. We had a 12-foot bush right in front of where a center-entrance front door would be, luckily we have a rear entrance. Some sites have a huge bush right at the back of the pad, blocking the ocean view. On the positive side, the beach is just a 10-minute walk away and it is spectacular. You can walk for miles, look at tide pools, watch the surfers, and get lost. The nearby towns of Laguna Beach and Newport Beach are great for shopping and eating. My rating is a combination of a "10" for location and a "5" for the campground itself. We camped at Crystal Cove State Park Moro Campground in a Fifth Wheel.
Date of Stay: February, 2014 - $35.00
Talk about RV heaven! This exquisite park overlooks a 3.5 mile beach. We had site 42, and we looked over an amazing cove. There is easy access to the beach from the campground. The whole park is immaculately kept. Can't wait to return.. We camped at Crystal Cove State Park Moro Campground in a Travel Trailer.
Date of Stay: January, 2014 - $55.00
This park has a lovely view of the Pacific, but access from the campground is through the day use area and a tunnel under the coastal highway. It is a steep walk down and back to the day use area, but doable for most people. Sites are adequate, although asphalt is crumbling a bit and some shrubbery hides part of the view. Some sites also could use some leveling. A problem is that I made reservations in the summer and prepaid $75 per day. Sometime in the fall they dropped the daily price to $55, but did not notify me and would not refund the difference. They suggested I should have cancelled and remade my reservation or come in on a walk-in basis. Also, be warned that they do not allow you to occupy your site before the 3:00 pm check-in time regardless of how long it has been empty. I had to drive down the beach and sit in my RV for over two hours waiting until the magic hour. This does not make sense because there cannot be that much "cleaning" of sites needing to be done, especially when they have been empty for several hours or days. We camped at Crystal Cove State Park Moro Campground in a Motorhome.
Date of Stay: October, 2013 - $75.00
Five nights Monday thru Friday nights. Quiet and the place did not ramp up on the weekend. Not a party campground at all. Most of our neighbors were retirees. Very quiet and overall a very nice park (for the money!). Like most Cal State Parks, maintenance to the facility seemed non-existent. Overgrown weeds and dead plants all over. Restrooms clean, but no evidence of any ongoing maintenance and in a few years this relatively new park will likely look like the rest of them. We would certainly stay here again and camped in a trailer. We camped at Crystal Cove State Park Moro Campground in a Travel Trailer.
Date of Stay: June, 2013 - $77.00
This is a great beach campground, although a bit pricey. Some sites are more level than others, and our site, #11 was level, but way over grown with shrubs. I trimmed some back a bit to get the slides out on the rig. I will stay here again. We camped at Crystal Cove State Park Moro Campground in a Fifth Wheel.
Date of Stay: February, 2013 - $68.00
This is a wonderful State Park campground. A beautiful view of the ocean from every site, an asphalt pad to park on, and electric and water available in the hook up spots. There is a dump station on site. The sites at the south end of the park are on more of a slope, so bring blocks for leveling. The park is terraced in rows. The upper rows are hook ups and the lower rows are dry camp. The 38' vehicle length limit is enforced. Even though the sites are back in, the rangers don't mind if you head your motor home in to get the view of the ocean from your front window. Since the sites are just electric and water, it is easy to run the utilities under your rig to the hook up pedestal. I will stay here again. We camped at Crystal Cove State Park Moro Campground in a Motorhome.
Date of Stay: October, 2012 - $38.00
This was our 2nd visit to this park which has outstanding views of the ocean from most sites. It is a steep but short walk or bike ride to the day use beach area, but you can also just drive down there with ample parking. There are shade ramadas and tables for picnics along with showers and restrooms in the day use area. You can hear the waves crashing on the beach at night from the campground which is very relaxing, along with the coyotes in the canyon singing their lonely hearts out. It is a close drive to everything. We camped at Crystal Cove State Park Moro Campground in a Travel Trailer.
Date of Stay: May, 2012 - $80.00
This park is very clean, restrooms cleaned every day and campsites are well kept too. All dogs welcome. There are three sewer dump sites so the lines to dump are super quick! We camped at Crystal Cove State Park Moro Campground in a Motorhome.
Date of Stay: May, 2012 - $75.00
This campground has the most amazing views and huge, wide spots. It should as it's one of the most expensive campsites that we've ever stayed in at $75/night for partial hookups. The location is great, and the Crystal Cove State Park and beach were really nice. I love how they tiered the campsites so that everyone had an awesome view of the ocean. We watched whales and dolphins swim by each day. The major cons were the price and the weeds. Our campsite was covered in weeds which seems silly for a campsite that costs so much. The park rangers and volunteers were very friendly (minus one gal) and the access to the beach is right there at the campground. Overall it was a good stay and spectacular views. We camped at Crystal Cove State Park Moro Campground in a Travel Trailer.
Date of Stay: September, 2011 - $32.00
Very nice place to stay for a few days as long as you don't want to camp right on the beach. The beach is close enough but it is a steep paved walk but you can drive down and back up very easy. The campground is the states newest so the vegetation between sites is small. I stayed in site 12 which is an accessible and it can be a little tight for backing in especially if you have the max size rig of 38' which I don't. Be sure and read all of the restrictions before booking, there are a lot of them. Awesome views of the ocean-at-large. Saw many pods of dolphins during the day feeding in the surf as I walked the beach. There were two men surf fishing with no luck. There are no shells to collect on the beach. The beach is excellent for swimming. We camped at Crystal Cove State Park Moro Campground in a Travel Trailer.
Date of Stay: August, 2011 - $60.00
This park just opened July 2011. Spacious sites all with ocean views. Beach access is by the day use area which is down a steep road adjacent to the campground. We preferred to drive our truck and park, instead of walking. Rangers patrolled the park on a regular basis. We enjoyed our visit and plan on returning again. We camped at Crystal Cove State Park Moro Campground in a Travel Trailer.
Date of Stay: August, 2011 - $68.00
Campground accessibility, POOR. The campground is located off Pacific Coast Hwy and is only accessible going northbound. We arrived going southbound and had a hard time turning around with a 37' 5th wheel in Laguna Beach. The Reserveamerica website does not have any driving instructions for this campground, just GPS coordinates. The entrance is poorly marked. The roads leading into, and within the campground are small and not suited to large trailers. We had to drive over landscaping going around the very tight corners. Our site (#12) was on the end of a row which had a left hand corner just past the site we were to back into. It was impossible to back a large trailer into this site. The road was too narrow and there were large rocks and and other permanent hazards along the road which made it impossible to swing wide enough to get into the site. We had to forfeit staying there. On the good side, the campground had a nice view of the ocean, the spaces looked large and well spaced from each other. With a smaller trailer or motorhome this looks like a good place to stay. It appeared we could have maneuvered into some of the other spaces, but there is not a lot of room to maneuver into any of the spots. For a brand new campground, I think this one was poorly designed. The sites should have been angled not straight in, the roads are way too small, accessibility is poor, the price too high and the the campground itself could have been laid out more randomly instead of all sites lined up in a line, there is plenty of space in the surrounding area. We camped at Crystal Cove State Park Moro Campground in a Fifth Wheel.
Date of Stay: July, 2011 - $65.00
We arrived thirty minutes early at the kiosk and were told we would have to turn around and come back a half hour later, no exception. Its a little hard to find a place to park a 37 foot motorhome in Laguna Beach. I did notice during my five day stay that they did turn away about ninety percent of campers who arrived before 3:00 PM, though they let a few in early. I did however speak to campground host who explained to me why the 3 PM check in. Had the young lady working the kiosk explained it to me instead of being so rude I would have understood. In addition to the high rate of $65.00 a night for staying at a state park that was not right on the beach such as Bolsa Chica, the price of $ .75 for a three minute shower was also a little high since most California State parks I stayed in charge $ .25 for 2 1/2 minutes. The road into the park is narrow and you must yeild to on coming traffic, plus roads in the campground are narrow due to the planting of native plants on one side of the road which are already showing signs of being ran over by campers trying to back into their sites. The size of the camp sites were nice and large with a wire cable surrounding each site. The rules say no open camp fires allowed but I did see a couple down below in the tent area. We camped at Crystal Cove State Park Moro Campground in a Motorhome.
UndoPre-Register below to access free and enhanced features coming in the near future.
Access to marketing tools, ability respond to reviews and monitor performance as well as expanded listings.
More Information about our Campground Owner Management and its enhancements are coming very soon. Features subject to change.


text:
Apprentice stars below par with OAP experiment
The Apprentice stars Nick Hewer and Margaret Mountford helped BBC1's The Town That Never Retired win the 9pm slot – although it was almost 2m viewers down on the slot average.
To continue reading, subscribe below with 25% off!
We are currently offering 25% off the subscription package of your choice. Prices will be increasing in 2015, so subscribe before 31st Dec 2014 with our discount to save 25% AND beat the price increase. This will be your last chance to access Broadcast at this great price.
Already a subscriber?
If you are a Broadcast subscriber already, simply sign in with your email address below:
Not a Broadcast subscriber?
Sign up today to save 25% and beat the 2015 price rise!
Find out more about all the benefits of a subscription to Broadcast.


text:
Meet AARP Tax Advisor John Kure, AARP Tax Coordinator Gene McCracken, former Lakewood Librarian Sharon Partridge and Lakewood Library Patron Rick Caulfield. These folks had a very happy experience last year!
Rick came to the library for AARP Tax Help, and John and Gene helped him find $2,000 in missed deductions! In the course of their review of his tax return, they pointed out that Rick had missed a $500 pension deduction. They not only helped him get a larger return in 2014; they also helped him file amended tax returns for previous years, resulting in overall savings of $2,000!
Don't make the same mistake! Let AARP's certified tax advisors help you with your tax returns this year.
Beginning in February, the Library and AARP will provide free tax counseling and preparation assistance to all taxpayers, with special attention to those ages 60 and older. IRS-certified volunteers will be on hand at our Arvada, Belmar, Columbine, Evergreen, Lakewood and Standley Lake Libraries to help you with tax-related questions through April 15. Space is limited for each session and participants are encouraged to arrive early. Please note: appointments are required at the Evergreen Library.
Arvada LibrarySchedule
Belmar LibrarySchedule
Columbine LibrarySchedule
Evergreen LibrarySchedule
Lakewood LibrarySchedule
Standley Lake LibrarySchedule
JCPL continues to partner with professionals from Jefferson County Human Services and the Mountain Resource Center (MRC) to assist patrons with signing up for health insurance through the Affordable Care Act on the Connect for Health Colorado online health insurance marketplace.
Personal assistance will be available at the following dates and times:
Belmar Library1-5 p.m. Friday, Jan. 99 a.m.–1 p.m. Monday, Jan. 124–8 p.m. Wednesday, Jan. 151–5 p.m. Friday, Jan. 164–8 p.m. Wednesday, Jan. 211–5 p.m. Friday, Jan. 239 a.m.–1 p.m. Monday, Jan. 264–8 p.m. Wednesday, Jan. 28
Evergreen Library11-3 p.m. Tuesday, Jan. 13, 20 and 27Evergreen appointments may be made by calling the MRC at 303-838-5772.
Knowing other languages and understanding other cultures is an important 21st Century skill. If you're interested in learning and practicing Spanish in a comfortable and friendly environment, check out our Intercambio Language Exchange. Now offered at both our Belmar and Wheat Ridge libraries, these informal sessions offer Spanish- and English-language learners the chance to chat with others and improve their skills through conversation. Todos son bienvenidos!
Belmar Library6:30 p.m., second and fourth Wednesday of the month
Wheat Ridge Library6:30 p.m., first and third Wednesday of the month
Looking for some camaraderie, intellectual stimulation and just plain fun? Check out our book groups.
We have books groups for adults, teens and kids! Titles include popular fiction, classics, autobiography, and more! You can view the full list of options here.
Come join us for some literary conversation!
Arvada Library
Tuesday Night Book Group6:30 p.m., first Tuesday of the month
Belmar Library
Page Turners Book Group6 p.m., first Wednesday of the month
Columbine Library
Tuesday Afternoon Book Group2 p.m., first Tuesday of the month
Young Readers' Fun Club (ages 8 and older)4:30 p.m., first Tuesday of the month
Teen Book Club (ages 12-18)3:30 p.m., one Saturday per month
Conifer Library
Award Winning Book Group6:30 p.m., last Tuesday of the month
Evergreen Library
Wednesday Night Book Group6 p.m., second Wednesday of the month
Young Readers' Book Club (ages 8-11)4 p.m., third Monday of the month
Golden Library
Tuesday Night Book Group6 p.m., third Tuesday of the month
Young Readers' Fun Club (ages 8-11)4 p.m., last Wednesday of the month
Lakewood Library
Tuesday Afternoon Book Group1 p.m., fourth Tuesday of the month
Thursday Night Book Group6 p.m., first Thursday of the month
Young Readers' Fun Club (ages 10 and older)4 p.m., third Tuesday of the month
Standley Lake Library
Tuesday Afternoon Book Group1 p.m., first Tuesday of the month
Thursday Night Book Group6:30 p.m., second Thursday of the month
Wheat Ridge Library
Thursday Morning Book Group9 a.m., third Thursday of the month
Wondering what to do while your kids are out of school? Bring them to the Library for Winter Break Fun! Our Arvada, Belmar and Columbine libraries are hosting movies and/or hands-on activities for kids to chase away those winter break blues.
Arvada Library1:00 p.m. Monday, Dec. 22 Tuesday, Dec. 23 Friday, Dec. 26 Monday, Dec. 29 Tuesday, Dec. 30 Friday, Jan. 2
Belmar Library1:00 p.m. Monday, Dec. 22 Tuesday, Dec. 23 Monday, Dec. 29 Tuesday, Dec. 30
Columbine Library (for kids, grades K-5) 1-5 p.m. Monday, Dec. 22 Tuesday, Dec. 23 Monday, Dec. 29 Tuesday, Dec, 30 Friday, Jan. 2 Monday, Jan 5
As a bilingual English/Spanish librarian, I often hear from adults that they studied Spanish in high school, but they remember very little from those days. It makes perfect sense when you look at brain development. When babies are born, about 15% of their brains have developed. By the time a child is 3, 85% of the brain has developed.
Researchers have found that by 6 months old, babies are already showing a preference for a certain language. Baby brains are wiring to the rhythms and sounds they hear from their families, caregivers and community. More studies go on to say that the best window of opportunity to learn a second (or third, or fourth...) language is between 0-7 years old.
Learning another language by the time we reach middle or high school can be too late!
Our corpus collosums (the part of the brain that connects the left and right side of the brain) grow harder as we age. Connections from one side to the other are no longer as quick as they are in young children when the corpus collosum is soft and malleable. Learning new things becomes more difficult. And, as we get older, we learn more and more information. Our brain starts pruning away at unused information. Ever hear the phrase 'Use it or lose it'? That's what our brain is constantly doing; trimming away at what it doesn't see as useful to us any more.
So why teach a child another language? For one, it has amazing affects on learning new concepts and problem solving! People who know more than one language can quite literally think 'outside the box' more readily than a monolingual or one language speaker. That's because they already think in different languages or in more than one way!
Also, younger learners can learn how to produce the native sounds of another language much easier than older learners. Think of the early wiring to language sounds as babies and the pruning the brain does as we age. When we are young, the brain is activated to learn as much as it can, including how to form sounds with our mouths and tongues. For example, as children, if we don't have an experience rolling an 'rrrr' (I used to mimic my cat's purring), we will have a difficult time later in life trying to learn how to do it. The brain is more open to learning how to produce sounds during the early years or this critical period in its development. Wow! As a former preschool teacher, this stuff facinates me!
Here's an easy book in English and Spanish with bright pictures of familiar foods to check out:
Over the next few months, I will be exploring more about second language learning and sharing ideas on teaching your child another language---even if you don't know another language yourself!
If you're looking for ideas or want to get started right away, come to Bilingual (English/Spanish) story times or ASL (American Sign Language) story times at Belmar Library!
Carrot photo credit: www.alternativa-verde.com
Try screen printing in this special instructor-guided session at the Belmar Library. Design your own image and print it using LumiPaint sun-activated dye. Bring your own shirt, hoodie, or fabric to print on, or use ours. Attendance is limited to the first 20 registered participants; register now. We also offer an open lab, where participants can work on their own projects. We offer access to soldering irons, other makers, and various tools, plus a place to work on your projects. No registration required.
These events are designed for adults; children may attend with an adult supervision.
LumiPaint Screen Printing10 a.m.-12 p.m. Saturday, Dec. 13
OpenLab 2-4 p.m. Saturday, Dec. 13
Everyone wants to be and stay healthy. To that end, staff from Jefferson County Human Services will be available through December to help you learn about and sign up for health insurance through the Affordable Care Act on the "Connect for Health Colorado" online health insurance marketplace. Appointments are not required, but can be made by calling 303-271-4691.
Personal assistance will be available at the Belmar Library at the following dates and times:
Appointments are not required, but can be made by calling 303-271-4691.
We love our patrons, and the holidays give us a perfect chance to say thank you! We've scheduled a number of holiday open houses to share some fun and festivities with you. Come celebrate with us!
5:30 p.m. Tuesday, Dec. 2Live music and refreshments.Arvada Library
5:00 p.m. Thursday, Dec. 4Face painting and a musical performance by Heart Strings.Wheat Ridge Library
1:00 p.m. Saturday, Dec. 6Live music and refreshments.Belmar Library
5:00 p.m. Friday, Dec. 12Music and after-hours festivities.Edgewater Library
1:00 p.m. Saturday, Dec. 13Music and refreshments and used books for sale.Evergreen Library
12:00 p.m. Sunday, Dec. 14Showings of the movie Frozen and refreshments.Columbine Library
Get ready to start the New Year with a new career! We're offering a number of job readiness classes to help you find your dream job. With classes ranging from standard resume techniques to job searching and interviewing skills, we can help you sharpen your skills and find the perfect job for you. These classes are offered at the Arvada and Belmar Libraries in partnership with the American Job Center.
Registration is required for these free workshops. Sign up now!
Arvada LibraryDec. 1 10:30 a.m.–12 p.m. Standard Resume TechniquesDec. 11 12–1:30 p.m. Interviewing for SuccessDec. 15 2–3:30 p.m. Job Search Tips & Tricks
Belmar LibraryDec. 3 12:30–2 p.m. Resume Review LabDec. 9 1–2:30 p.m. Job Search Tips & TricksDec. 18 12:30–2 p.m. Interviewing for Success


text:
Sage Hospitality, Whitman Peterson Acquire 11 HotelsTuesday February 25th, 2014 - 9:54AM E
| | | | | | | | | | | These are shortcuts to your favorite social networking and bookmark sites. Add this story to your Facebook page, del.icio.us, DiggIt, and many others!
DENVER—Sage Hospitality has purchased 11 hotels in partnership with Whitman Peterson, marking the second of an investment venture between the two companies. Sage Hospitality and Whitman Peterson plan to acquire a sizable portfolio of select-service hotels in need of high-impact capital, operational, and/or brand improvements. The first portfolio, purchased in March 2013, encompassed seven branded, but under-capitalized hotels and is in the final stages of renovation. This new transaction adds 11 hotels to the venture, bringing Sage's total hotels under management to 76 nationally. The newly added properties are located in several metropolitan areas strategic to the venture, including Austin, Denver, Indianapolis and Dallas. "The properties are geographically diverse and encompass a variety of top tier brands in the Marriott, Hilton, and Hyatt systems," stated Michael Everett, chief investment officer for Sage Hospitality. "We see significant upside from capital expenditure and operational improvements, which is perfectly inline with our investment strategy." Everett continued, "With an acquisition price of approximately $70,000/key and an approximately 10% cap rate on trailing 12 income—we believe this portfolio offers a unique combination of in-place yield and attractive upside, at a basis after renovation that is well below replacement cost." Tags: Sage Hospitality • Whitman Peterson • Hospitality • Acquisitions • |
As we begin 2015, and what is expected to be a healthy year for the hotel industry with expansion, progress and innovation as key catalysts for growth, Hotel Business is right on board with that momentum, bringing you, with this issue, our own updated product.


text:
Deal of the week: IPS monitors with 4K and 1080p resolutions Nvidia's new GeForce GTX 960 is a tempting candidate for the deal of the week, especially since cards are already selling for $199.99... Type: News story Tags: Displays |
11 |
Report: Acer prepping curved 34'' display with G-Sync Fans of curved, ultra-wide displays may soon be able to get one with Nvidia's variable refresh mojo. According to TFT Central, Acer is developing... Type: News story Tags: Displays Gaming |
32 |
Microsoft's next-gen browser has annotation system, Cortana assistant Microsoft's rumored Project Spartan browser is real, and it's coming to Windows 10 devices, including smartphones. This apparent heir to Internet Explorer has a... Type: News story Tags: Internet Software |
30 |
Windows Holographic can put you on Mars, in Minecraft For years, a skunkworks team at Microsoft has been quietly exploring augmenting reality with virtual holograms. The fruits of their efforts were unveiled during today's... Type: News story Tags: Graphics Displays Gaming |
59 |
Steam update adds non-beta Steam Broadcasting, FPS counter A new version of Valve's Steam client has been released. The list of changes and additions is fairly long; most notably, the update makes... Type: News story Tags: Gaming Software |
22 |
In the lab: BenQ's XL2420G G-Sync monitor After a busy week of CES coverage, I'm back in the lab with some shiny new hardware to review. This time, I'm looking at BenQ's... Type: News story Tags: Displays |
67 |
Analyst expects Apple to start transition away from Intel by 2017 No question about it, Apple's homebrewed processors for the iPhone and iPad are getting mighty fast. According to an analyst quoted by AppleInsider, those chips... Type: News story Tags: CPUs Systems Mac |
202 |
Biostar teases Z97 motherboard with USB 3.1 MSI isn't the only one prepping a Z97 motherboard with USB 3.1 connectivity. Biostar also has one in the works. The Gaming Z97X first... Type: News story Tags: Motherboards |
14 |
Seiki adjusts plans for 4K desktop monitors This summer, affordable HDTV maker Seiki announced that it was getting into the monitor business with a trio of 28-40" displays boasting VA panels,... Type: News story Tags: Displays |
14 |
Report: Win10's Spartan browser to have tab grouping, Cortana, annotations Last month, the rumor mill broke the news about Spartan, a lightweight web browser Microsoft is said to be developing for Windows 10. The... Type: News story Tags: Internet Software |
32 |
Here's 240-FPS footage of AMD's FreeSync tech—and some new info We've been hearing about FreeSync, AMD's answer to Nvidia's G-Sync variable refresh display tech, for just over a year now. This week at CES,... Type: News story Tags: Graphics Displays Gaming |
130 |
Deal of the week: A 1080p IPS monitor for $100 and 802.11n Wi-Fi router for $5 We interrupt this week's deluge of CES stories for a look at some of the best PC hardware deals available right now. IPS monitors typically carry... Type: News story Tags: Storage Displays Networking |
12 |
Here are more glamor shots of MSI's USB Type-C motherboard MSI's Z97A Gaming 6 is the first motherboard we've seen with a USB 3.1 Type-C port. The official MSI photo we posted the... Type: News story Tags: Motherboards |
22 |
Razer delves into VR with the OSVR initiative and $200 Hacker Dev Kit We told you about Razer's Forge TV yesterday, but that's not the only news from the company this week. Razer has also teamed... Type: News story Tags: Displays Input devices Personal tech |
1 |
Asus' next jumbo 4K monitor will be ''way cheaper'' Asus' 31.5" PQ321 was one of the first 4K desktop monitors on the market. The display's dual-tile nature is a little awkward, though, and... Type: News story Tags: Displays |
12 |
Asus' MG279Q display melds IPS panel, variable refresh We already told you a little bit about Asus' 27-inch MG279Q monitor earlier this week. We've now learned more about it—and Scott has seen... Type: News story Tags: Displays |
35 |
Dell's XPS 13 ultrabook boasts amazingly thin bezels, 15-hour battery New laptops are everywhere at the Consumer Electronics Show this year, but Dell's XPS 13 easily stands out from the crowd. The Broadwell-based system... Type: News story Tags: Displays Mobile computing |
43 |
Gigabyte's new X99 board is an overclocking champion Gigabyte has introduced another high-end Haswell-E motherboard aimed at competitive overclockers. During its CES press conference today, the firm revealed the X99-SOC Champion, which managed... Type: News story Tags: Motherboards |
12 |
Nvidia admits, explains GeForce GTX 970 memory allocation issue | 205 |


text:
From the D
New Year's Eve doesn't get much bigger than this: Kid Rock will headline extended weekend festivities at Hard Rock Live at Seminole Hard Rock Hotel & Casino. The Detroit rocker will perform two shows; one on Sunday, December 30 at 8 p.m. and one on Monday, New Year's Eve, December 31 at 9:30 p.m. Post show, keep the energy flowing late night with a variety of non-stop party packages at the Seminole Hard Rock Hotel & Casino. The ex Mr Pamela Anderson's Born Free went platinum and its self-named title track became a signature song for Kid Rock, hitting the top tiers of the radio charts and becoming a concert staple.
Rock & Roll Jesus (2007), Kid Rock's previous album, debuted at #1 and stayed on the charts for 100 weeks, becoming the biggest selling Rock album of 2008. His single "All Summer Long" hit #1 in eight countries. Kid Rock's recently released Rebel Soul and its lead single, "Let's Ride (Mideast Battle Hymn)," is already receiving critical acclaim.
Tickets are $150 and $100*; all seats are reserved and available at all Ticketmaster outlets online at www.ticketmaster.com or 800-745-3000.
[1] http://www.miami.com/on-our-radar
[2] http://www.miami.com/new-store-openings-aventura-mall-article
[3] http://www.miami.com/shopping/haute-happenings
[4] http://www.miami.com/beer-pigskin-skin-more-pigskin-music-more-beer-amp-super-bowl-article
[5] http://www.miami.com/nightlife/velvet-underground
[6] http://www.miami.com/manny-pacquiao-floyd-mayweather-heat-game-article
[7] http://www.miami.com/jookin-lil-buck-miami-article
[8] http://www.miami.com/glamorous-red-carpet-miss-universe-article
[9] http://www.miami.com/christian-slater-shows-party-article
[10] http://www.miami.com/shaq-basketball-law-enforcement-article
[11] http://www.miami.com/amber-rose-makes-splash-miami-article
[12] http://www.miami.com/shannen-doherty-talks-new-reality-show-article
[13] http://www.miami.com/brazilian-soccer-star-keeps-party-going-article
[14] http://www.miami.com/joanna-krupa-suing-fellow-housewives-star-article
[15] http://www.miami.com/magic-johnson-book-appearance-article
[16] http://www.miami.com/justin-bieber-misses-another-hearing-article
[17] http://www.twitter.com/miamicom


text:
what is this?
Question is Closed | Report Mark as Favorite |
Answers (1)
Search for questions
Still looking for answers? Try searching for what you seek or ask your own question.
Similar questions
What is this pill - with '5434 on one side and dan/dan on other,small,white and round?
1 answer • 3 Aug 2009
What is this pill - with 'DAN DAN 3442' imprint?
White round tablet one side scored DAN DAN other side 3442 please help identify thanks
1 answer • 18 Dec 2009
What do DAN pills look like?
WHITE ROUND one side says DAN the other says 5658
2 answers • 18 Jan 2010
What do prednisone pills look like?
What is the little green predisone pill called? They gave me it last night at the hospital now they prescribed me with the same predisone just a ...
0 answers • 18 Dec 2011
What is dan dan 5443?
1 answer • 12 Apr 2012


text:
LEAP Grant applications are live! Our Literacy and Education in Action Program […]
by Craig Wiener
Hello, I'm an eBook! ATTENTION: This item is an eBook. It can be read on iOS, Android, MAC and PC's with a supported eReader. It is not a physical book. eBooks are available via download immediately after you've checked out.
Condition: Used Very Good
Shipped from other seller
Converts one physical book into a digital version. Learn More
eDelivery converts your used book order into a digital version readable on most devices. Learn More
Ships directly from Better World Books
HPB-Flagship TX, USA
CheaperReader GA, USA
Nataraj Books VA, USA
Alibris NV, USA
It is now possible for concerned parents to treat their child's attention deficit/hyperactivity disorder (ADHD) without medication. This book shows them how. Written by a supervising psychologist who specializes in child behavioral issues, Parenting Your Child with ADHD presents a groundbreaking program for parents seeking to reduce their child's inattention, hyperactivity, and impulsivity and strengthen his or her capacity for self-reliance and cooperation. This unique program promotes positive ways of interrelating and shows parents the five main ways they may have unwittingly reinforced ADHD symptoms in the past. Far from encouraging parents to strictly manage ADHD children, this approach promotes independence in kids so that less discipline and surveillance is necessary.
Specifically, this book helps parents promote cooperation by phrasing their requests in specific ways, reduce pressure on their child, and acknowledge the child's preference as a way to make cooperation a more attractive choice to him or her. Parents learn to resolve problems related to messiness, inappropriate silliness and intrusiveness, chores and helping family members, coordinating schedules, sneaking and stealing, noise and yelling, and overreacting. The second part of the book moves on to issues that take place outside the home, such as shopping excursions, family outings, and travel. Finally, parents learn ways to increase their child's independence and cooperation with schoolwork and compliance in the classroom environment. The child learns the value of being knowledgeable without the motivation of punishment or gift rewards.
Our best deal on used books 3 for $10 and just $3 each additional book. Shop and Save
A great last-minute holiday present. Exactly what they want to read. Order Gift Certificates »
We match every book you purchase with a book donation. Learn more »


text:
Schwans - credit
- Jan 21 2015-01-21 13:51:31
The Schwan Food Company is a privately held company. The company was founded in 1952 by Martin Schwan as an ice cream home delivery. Schwan Food Company is headquartered in Marshall, Minnesota. The Schwan Food Company specializes in production, marketing and distribution of more than four hundred kinds of frozen foods. Schwan Food Company sells its products in more than fifty countries all over the world. Schwan's has several corporate divisions that include Schwan's Home Service, Global Consumer Brands, Global Food Service, and Global Supply Chain. The first division provides food directly to home.C/O Customer Service Marshall MN 56258
- Food Delivery
- Prescott, Arizona
- Return Charge
- 2
- 92
I gave them authorization to charge my bank account on the 30th of December and unfortunately there wasn't the amount of money in my account and it was returned back to Schwan's but by the 7th of Jan that payment cleared. But they decided to charge me $25 for the return charge. I I called and they told me I was a valued customer probably for more than 6 years but they would not credit my... Read more ›
Was this review helpful? 0 1 4


text:
Berry glitch
This glitch is in need of research. Reason: Needs the exact cause of this glitch You can discuss this on the talk page. |
The Berry glitch is a glitch only found in the coding of Pokémon Ruby and Sapphire which seems to stop the game's day counter. The name of the glitch comes from the fact that the first thing a player will usually notice is that all Berries that have been planted but have not been harvested have ceased growing. The glitch typically occurs after about a year of owning either game, or to games that have been played over 100 hours, but the exact mechanics of its cause are currently unknown.
Other affected areas
As the Berry glitch effectively freezes the game's day counter, other areas of the game are also affected by the glitch:
- Lilycove Dept. Store will never have any sales.
- Lilycove Dept. Store will stop allowing the player to draw a Lotto Ticket.
- The Mauville Game Corner will never have any service days.
- The Energy Guru will never sell vitamins cheap.
- The tide in Shoal Cave will never change.
- The man who gives out TM27 (Return) and TM21 (Frustration) in Pacifidlog Town will stop giving out TMs.
- NPCs that hand out free Berries once per day will stop handing them out.
- Mirage Island's random number will never change.
- Eevee will only be able to evolve into one of Espeon or Umbreon and not the other.
- Route 119 and Route 123 will be stuck with the same weather condition.
Similar effects with dead battery
The glitch results in problems very similar to what happens when the game cartridge's CR1616 internal battery runs dry, which stops the game's in-game clock. However, in cartridges that have a dead battery, a message will appear after the title screen that warns of the dry battery and the fact that clock-based events will no longer occur. In contrast, games being affected by the glitch will not display this message. Cartridges that report a dead battery may or may not be also afflicted with the berry glitch; in this case, methods to fix one problem will not fix the other problem, which can make it look as if the fix has not worked. It can also cause the illusion that an Emerald cartridge is affected by the glitch. In order to fix a dead-battery issue, the battery must be physically replaced.
Similar effects after replacing a dead battery
This section is incomplete. Please feel free to edit this section to add missing information and complete it. Reason: Unclear/confusing, could use rewording. |
Cartridges that had their battery replaced may experience similar affected areas. The RTC chip's value is reset to a value prior to the "last-saved-field" of the savegame thus causing the game to not register time as passed. Most of the effects mentioned rely on countdowns and will still be visible on cartridges after the battery has replaced. This may cause the illusion that a cartridge is affected by the glitch when it is not.
However, the clock in the player's room and the Eevee evolution are based on "real time" and therefore be correct. Thus, if a cartridge allows different Eevee evolutions and shows time passing on the clock in the player's room but the other effect are visible. Shoal cave is a special case as it includes both dependencies: The tide will change (dependent on "real time") but Shoal Salts and Shoal Shells will not be refilled (dependent on timeouts)
These issues can be fixed by creating a new savegame (and therefore resetting the "last-saved"-field). Using additional hardware, it may be possible to set the RTC value or edit the "last-saved"-field in a savegame.
Fixing the glitch
The glitch can be fixed with the Berry Program Update from Pokémon FireRed, LeafGreen, and Emerald, a patch from Pokémon Colosseum, Pokémon XD, Pokémon Channel, Pokémon Box Ruby & Sapphire, the e-Reader, or a specific interactive demo disc, as well as by sending the affected game to Nintendo.
Pokémon FireRed/LeafGreen/Emerald patch
- Main article: Berry Program Update
- Load FireRed, LeafGreen, or Emerald until the title screen is displayed.
- Press the Select and B buttons. After the new screen loads, press A.
- Insert the Link Cable so that the system that the FireRed, LeafGreen or Emerald game is connected to the Player 1 cable port and the Ruby or Sapphire game is connected to the Player 2 port. Once this is done, press A.
- Turn on the device with Ruby or Sapphire while holding the Start and Select buttons.
- Refer to the FireRed, LeafGreen or Emerald game display for further instructions. The patch should be transferred at this time.
Pokémon Colosseum and XD patch
Linking an affected game to Colosseum or Pokémon XD for trading or battling will automatically download the patch. In addition, the Bonus Disc that came with pre-orders of Colosseum can be used to download Jirachi into a game; when this happens, the patch is downloaded as well.
Pokémon Channel patch
Downloading Jirachi from a PAL version of Pokémon Channel will automatically download the patch.
Pokémon Box patch
Linking an affected game to Pokémon Box Ruby & Sapphire, accessing the Go To Adventure mode, and then saving the game whilst playing will also automatically fix this glitch, with the message "The Berry Program was updated" appearing with the save confirmation. (Note that this option is not available for Japanese versions.)
e-Reader patch
For Japanese versions, a special set of e-Reader cards (ID# 16-A001 & 16-A002) were distributed with Pokémon Scoop's Winter 2004 issue to allow players to fix this glitch by using e-Readers.
Interactive demo patch
Specially-marked demo discs were released to selected stores across U.S. and Japan, so players can visit these stores and download the patch from the disc. By downloading the patch from a demo disc, player will also receive a Shiny Zigzagoon holding a Liechi Berry as a bonus. However, since the program does not block fixed games, players can receive multiple Zigzagoon by downloading to the same cartridge again.
Sending cartridge to Nintendo
If the player had no opportunity to download the patch, the game could be sent to Nintendo to have it fixed. However, as of August 2012, Nintendo stopped offering to patch the Berry glitch.
External links
This article is part of Project GlitchDex, a Bulbapedia project that aims to write comprehensive articles on glitches in the Pokémon games. |


text:
AFM study of complement system assembly initiated by antigen-antibody complex
ABSTRACT The shape and size of complement system C1 components assembled on a SiO2 surface after classical activation by antigen-antibody complex was determined by tapping mode atomic force microscopy (AFM).
The SiO2 substrate was silanized and bovine leukemia virus proteins gp51 were covalently bound to the SiO2 substrate. Self-assembly of complement system proteins was investigated by AFM. Uniform coating of silanized surface by gp51 proteins was observed by AFM. After incubation of gp51 coated substrate in anti-gp51 antibody containing solution, Ag-Ab complexes were detected on the substrate surface by AFM. Then after treatment of Ag-Ab
complex modified substrate by guinea-pig blood serum containing highly active complement system proteins for 3 minutes and
30 minutes features 2–3 times and 5–8 times higher in diameter and in height if compared with those observed after formation
of Ag-Ab complex, were observed respectively on the surface of SiO2. This study revealed that AFM might be applied for the imaging of complement system assembly and provides valuable information
that can be used to complement other well-established techniques.
- Yu. D. Ivanov, V. V. Danichev, T. O. Pleshakova, I. D. Shumov, V. S. Ziborov, N. V. Krokhin, M. N. Zagumenniy, V. S. Ustinov, L. P. Smirnov, A. V. Shironin, A. I. Archakov[Show abstract] [Hide abstract]
ABSTRACT: The atomic-force microscopy-based method of irreversible chemical AFM-fishing (AFM-IFchem) has been developed for the detection of proteins existing at ultra-low concentrations in solution. Using this method, a very low concentration of horseradish peroxidase (HRP) protein (10−17 M) was detected in solution. A theoretical model that allows the description of obtained experimental data has been proposed. This model takes into consideration both transport of the protein from the bulk solution onto the AFM-chip sur-face and its irreversible binding to the activated area.Biochemistry (Moscow) Supplement Series B Biomedical Chemistry 7(1). - SourceAvailable from: Arunas Ramanavicius[Show abstract] [Hide abstract]
ABSTRACT: Publications in fields of Biosensors, conducting polymers etc. - [Show abstract] [Hide abstract]
ABSTRACT: UV detectors based on surface-barrier structures and new kinds of photoresistive UV detectors have been developed. Their characteristics are presented in comparison with those of other structures.01/2003;
Page 1
???????? ????????
???????? ????????
???????? ????????? ??????????????? ????????? ???????
??? ?????????
? ?? ?? ?
??? ?????????
? ?? ?? ?? ?
? ?? ?? ?? ?
DOI: 10.1007/s11532-005-0015-8
Research article
? ?
? ?? ? ? ?? ? ? ?? ?
? ?
? ?? ?? ?
? ?
? ?? ?? ?
? ?
? ?
? ?? ?
AFM study of complement system assembly initiated
by antigen-antibody complex
Almira Ramanaviciene1,2, Valentinas Snitka3, Rasa Mieliauskiene1,
Rolandas Kazlauskas1, Arunas Ramanavicius1,2∗
1Department of Analytical and Environmental Chemistry,
Vilnius University,
Naugarduko 24, 03225 Vilnius 09, Lithuania
2Sector of Immunoanalysis and Informatics, Institute of Immunology,
Vilnius University,
Moletu pl. 29, 08409 Vilnius 21, Lithuania
3Research Center for Microsystems and Nanotechnology,
Kaunas University of Technology,
Studentu 65, 3031 Kaunas, Lithuania
Received 23 June 2005; accepted 15 November 2005
Abstract: The shape and size of complement system C1 components assembled on a SiO2
surface after classical activation by antigen-antibody complex was determined by tapping mode
atomic force microscopy (AFM). The SiO2substrate was silanized and bovine leukemia virus
proteins gp51 were covalently bound to the SiO2substrate. Self-assembly of complement system
proteins was investigated by AFM. Uniform coating of silanized surface by gp51 proteins was
observed by AFM. After incubation of gp51 coated substrate in anti-gp51 antibody containing
solution, Ag-Ab complexes were detected on the substrate surface by AFM. Then after treatment
of Ag-Ab complex modified substrate by guinea-pig blood serum containing highly active
complement system proteins for 3 minutes and 30 minutes features 2-3 times and 5-8 times
higher in diameter and in height if compared with those observed after formation of Ag-Ab
complex, were observed respectively on the surface of SiO2. This study revealed that AFM might
be applied for the imaging of complement system assembly and provides valuable information
that can be used to complement other well-established techniques.
c ? Central European Science Journals Warsaw and Springer-Verlag Berlin Heidelberg. All rights reserved.
Keywords: Bionanotechnology, antigen-antibody docking, complement system, bovine leukemia
virus, AFM
Page 2
A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206 195
1 Introduction
Biological self-assembling systems are governed by nanoscale processes that evolved over
millions of years, but only the most successful examples of biological systems have been
surveyed. In order to understand the functioning of biological systems, knowledge about
the character of biomolecular interactions and structure of formed complexes is crucial.
The convergence of nano-scale science with modern biology, medicine and bioanalytical
chemistry is a promising trend [1]. Nanotechnology provides the tools and technology
platforms for the investigation and transformation of biological systems, and biology of-
fers inspiration models and bio-assembled components to nanotechnology. Emulating the
concepts and principles of biology has led to the controlled self-assembly of biomateri-
als. Self-assembly and self-organization have inspired ideas for advanced bioengineering
methods at the nano-scale [2]. One of such unique self-assembling bio-nanomachines is a
complement system. The complement system is the major effector of the humoral branch
of the immune system [3] and plays an essential role in host defense against infectious
agents and in the inflammatory process [4, 5].
The complement system is composed of over twenty proteins involved in a sequence of
cascading reactions ultimately resulting in the elaboration of biologically active products
and destruction of the cellular membranes [6]. The complement system may be initiated
through the "classical pathway" by the binding of antibodies to cell surface antigens,
through the"alternate pathway"by the presence of foreign cell surface components, such
as polysaccharides, as well as antibody aggregates and throught the "lectin pathway"
by the mannan binding protein [7, 8]. The initial step in the activation of the classical
pathway involves interaction between the first component of complement, C1, and a
number of receptor sites present on the immunoglobulin molecules forming an complexes
[9]. Thus, the most important proteins for self-assembly of complement system are C1
proteins (C1q, C1s and C1r), which are involved in such general complex formation
processes: the complexing of antibody with antigen induces conformational changes in the
Fc portion of the antibody molecule that exposes a binding site for the C1 component of
the complement system; C1 (approximately 900 KDa) exists in serum as a macromolecular
complex consisting of C1q (400 KDa) and two molecules each of C1r (168 KDa) and
C1s (83 KDa), held together in a complex (C1qr2s2) stabilized by Ca2+ions. The C1q
molecule is composed of 18 polypeptide chains that associate to form six collagen-like
triple helical arms, the tips of which bind to exposed C1q-binding sites in the CH2 domain
of the antibody molecule. Each C1r and C1s monomer contains catalytic and interaction
domains; the latter facilitates interaction with C1q or with each other. Each C1 molecule
must bind, via its C1q globular heads, to at least two Fc sites for a stable C1-antibody
interaction to occur [10].
Following initial activation, various complement components interact, in a highly reg-
∗E-mail: firstname.lastname@example.com
Page 3
196A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206
ulated enzymatic cascade, to generate reaction products that facilitate antigen clearance
and generation of inflammatory response. The complement reaction products amplify the
initial antigen-antibody reaction and convert it into a more effective defense mechanism.
Finally, the terminal components of the complement system generate the membrane-
attack complex [11]. The set of proteins necessary to build a functioning complement
system is present in the blood of all mammalians. However, self-assembling of this sys-
tem starts only in the case if particular antigen-antibody complex is formed on the surface
of cell membrane where antigens are exhibited. Also, circulating Ag-Ab complexes are
able to fix complement system proteins. Due to complement receptors, such immune
complexes are delivered to the organs of the monocyte-phagocytic and reticuloendothe-
lial systems. This process can be affected by environmental contamination [12]. The
complement system of guinea-pig is especially active and is often used as a model system
for investigations of complement system function [13].
The function of specific actions in complement system is under intensive investiga-
tions: function of complement proteins [14–16]; structure and function of complement
activity controlling protein was predicted [5]; influence of various factors on regulation of
complement-mediated cytotoxicity [17]; inactivation of complement system [18]; etc. Be-
cause of the complexity of the complement system, the real structural model that might be
obtained by AFM will be useful for detailed investigations of this complex system. Appli-
cability of AFM for investigation of other biomacromolecules-based complex systems has
been demonstrated: for structural analysis of the reaction center light-harvesting complex
I [19], observation of Chitosan-induced restructuration of a mica-supported phospholipid
bilayer [20], study of single protein based systems [21], in vitro reconstitution of fibrillar
collagen type I assemblies at reactive polymer surfaces [22], visualization of band-like cel-
lulose assemblies produced by bacteria [23], characterization of surface-immobilized layers
of intact liposomes [24], morphology study of starch, amylose, and amylopectin films [25]
We believe that atomic force microscopy might be very useful for investigations of
complement system assembly and the aim of this study was to show that complement
system assembly might be investigated by means of tapping mode AFM.
2Experimental part
2.1 Chemicals
Bovine leukemia virus (BLV) proteins gp51 as well as the specific serum containing anti-
gp51 antibodies were obtained from 'Biok' (Kursk, Russia). Ethanol, 3-aminopropyl-
thriethoxysilane (APTES) (Sigma, St.Louis, USA) was used for SiO2substrate prepara-
tion. 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimid (EDC) (Sigma, St.Louis, USA) was
used for protein carboxyl group activation. Blood serum of guinea-pig containing active
complement system proteins and BLV not infected cattle blood serum were obtained from
the Institute of Immunology of Vilnius University (Lithuania, Vilnius).
Page 4
A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206197
2.2Silanization of SiO2substrate
SiO2substrate and test-tubes were washed by ethanol and dried by heating. SiO2sub-
strate was placed into test-tubes with APTES and heated for 1 hour at 90◦C. After
washing with water, SiO2substrate was prepared for protein immobilization.
2.3 Immobilization
50 mg/ml solution of gp51 in 0.9 % NaCl was prepared and activated with EDC solution
for 1 hour. Freshly prepared surface of 8˚A SiO2was exposed to activated antigen gp51
molecules for 7 h. Antigen solution used for immobilization gives an approximate area
density of 40-50 molecules per µm2. Active groups that were not engaged in formation
of covalent bonds were deactivated by 0.1 M glycine solution, pH 7.0. The surface was
then rinsed with water.
2.4Preparation of samples for AFM study
Before each AFM measurement the surface of modified 8˚A SiO2was rinsed with water and
dried using a flow of dry Argon gas. To study antigen-antibody interaction, the surface of
8˚A SiO2covered with immobilized antigens was exposed to antibody containing solution
for 30 min., rinsed, dried, and studied by AFM. To study antigen-antibody-complement
system interaction the surface containing antigen-antibody complexes was exposed to
guinea-pig serum rich with active complement system proteins for 1 h and then it was
rinsed, dried and probed by AFM.
Before each AFM-imaging samples were rinsed in 0.1 M glycine buffer, pH 7.0, for 5
min. to prevent non-specific interactions and then thoroughly rinsed in deionized water to
prevent crystallization/aggregation of glycine extent. Then samples were dried by flowing
argon gas.
2.5AFM imaging
High resolution tapping mode Atomic force microscope (AFM) measurements were per-
formed with a home-built AFM interfaced with NT-MDT Corp. (Zelenograd, Moscow,
Russia) control electronics and contact mode measurements were made by AFM"Q-Scope
250" Quesant Instrument Corporation (Agoura Hills, USA).
The dry samples were investigated by AFM in the contact and tapping mode in a range
of scan lengths from 5 µm to 1 µm. The commercial Si cantilevers NSG11 series (length
100 µm and width 35 µm) with a force constant 11 Nm−1and tip curvature 10 nm and
resonanse frequency 255 kHz (NT-MDT) were used for tapping mode measurements and
soft cantilevers with force constant 0.25 Nm−1were used for contact mode measurements.
The slides of 0.5 cm2surface area of 8˚A SiO2substrate received from AIXTRON AG
(Aachen, Germany), were used as substrates for AFM investigations.
Page 5
198 A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206
AFM images are presented in two formats: large format (Fig. 1 A,B,C,D) representing
characteristic image of 5000 × 5000 nm area of modified surface and detailed format (Fig.
3 A,B,C,D) with height width diagrams where characteristic features are analyzed.
For all AFM images 200 × 200 pixels image resolution was applied, scanning rate 10
3 Results and discussion
The immunoreagents bovine leukemia virus (BLV) protein gp51 and polyclonal anti-gp51
were selected for current experiments because of high affinity of these reagents [26] and
high stability of gp51/anti-gp51 complex [27, 28]. Moreover, BLV infections are still
frequent and detection of those infections as well as effective treatment methods are
important [29]. The experimental concept of the presented experiment was based on the
measurement of radius and height of features observed by scanning probe microscope,
because the height and radius of these features was dependent on the complex size of
immobilized/formed macro molecular species.
AFM imaging was performed in dry state which is a cheaper and more basic alternative
compared to AFM imaging in liquid environment [30]. AFM images of SiO2substrate
surface before and after silanization process seem to have no significant differences in
morphology (Fig. 1A) or any significant change in roughness presented in corresponding
histograms (Fig. 2A), therefore silanization insignificantly changes those properties of
the surface in applied resolution scale. After covalent attachment of gp51 proteins, the
differences in morphology (Fig. 1 B) and roughness (Fig. 2B) of substrate were detectable
at our AFM equipment resolution scale. From the image presented it clearly seems that
180-230 nm diameter and 7-9 nm height (Fig. 3A) features appeared on the surface
of silica. It is estimated that observed features (Fig. 3A) are bovine leukemia virus
protein gp51 and/or its complexes covalently immobilized on the surface of silica substrate
[31, 32]. The results obtained illustrate that success of covalent protein immobilization
can be estimated by AFM.
Optimal incubation time (30 min.) of gp51 modified surface with anti-gp51 Ab was
determined in previous experiments [33]. The AFM images (Fig. 1C, Fig. 3B) registered
after 30 min incubation of gp51 modified silica in solution containing anti-gp51 antibodies
showed significant differences in morphology (Fig. 1C, Fig. 3B) and roughness (Fig. 2C)
if compared with roughness (Fig. 1B, Fig. 3A) and morphology (Fig. 2B) registered
before this treatment or obtained by equally treated control SiO2surface which was not
covered by gp51 (data not shown). In images of mica surface investigated by AFM after
exposure of gp51 modified SiO2to anti-gp51 Ab containing solution (Fig. 1C, Fig. 3B)
two distinct populations of features with height-radius corresponding to those of gp51
and antigen-antibody (gp51/anti-gp51) complex are observed. If gp51 modified surface
was exposed towards solution containing set of other antibodies but not containing any
anti-gp51 observed features (Fig. 3E) were similar to those observed on the gp51 modified
SiO2surface before treatment with antibody containing solution. Expected gp51/anti-
Page 6
A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206199
gp51 complexes were larger in height-radius almost twice if compared with immobilized
gp51 proteins (Fig. 1B, Fig. 3A). The width-height of gp51/anti-gp51 complex was ap-
proximately twice higher what represents the range of Ag-Ab complex dimensions [34–38].
It allowed to distinguish between immobilized antigens and antigen-antibody complexes
formed. The formed features allow us to conclude that we observe the antigen-antibody
docking reaction, or the appropriate lack thereof, between single antigen molecules im-
mobilized on the surface and single or multiple antibody molecules. However, the yield
of such formations (surface area covered by 7-9 nm height features as presented in fig-
ure 2A was compared with area covered by 10-20 nm height features was measured and
compared) not exceeded 7-15 % if compared with suspected gp51 immobilization yield,
the main reason for it might be improper orientation of epitopes present in gp51, and
this is in good agreement with the results obtained during the AFM detection of Ag-Ab
complexes [39]. Since a high number (over 85 %) of immobilized antigen was not engaged
in the formation of antigen-antibody complex we conclude that not all antigen molecules
are properly oriented and/or denatured and they are unable to bind with Fab sites of
specific antibody.
When a surface without immobilized antigens was exposed to the same antibody
containing solution no clear features similar to those presented in Figures 1C and 2B
were detected. The AFM image was absolutely identical to the image of unmodified SiO2
substrate surface because in the absence of immobilized antigen specific affinity interaction
and strong binding of any proteins to the surface was not reliable, not specifically adsorbed
proteins were removed by 0.1 M glycine buffer, pH 7.0.
After 3 min. treatment of silica substrate modified with gp51/anti-gp51 Ab com-
plex in the complement protein containing solution, 40-70 nm diameter and 30-40 nm
height structures appeared on the surface (Fig. 1D and Fig. 3C). Height histogram (Fig.
2D) derived from figure 1D shows appearance of higher features if compared with those
presented in figures 2A,B,C derived from figures 1A,B,C correspondingly. No similar
structures were observed if unmodified silica substrate or silica modified just with gp51
was treated by the same complement system protein containing solution for the same
A significant increase in geometrical dimensions of the detected structure of previously
formed Ag-Ab complex might be the result of self-assembly of a number of C1 components
during early stage of complement system self assembly initiated by antigen antibody
After 1 h treatment of silica modified with gp51/anti-gp51 Ab complex in the com-
plement protein containing solution, sparse 500 nm diameter and almost 400 nm height
structures appeared on the surface (Fig. 1E and Fig. 3D). Appearance of significantly
higher features was confirmed by appearance of significantly higher structures in cor-
responding histogram (Fig. 2E). We believe that those structures were based on com-
plement system proteins self-assembled on the basis of Ag-Ab complexes, while no such
structures were detected if just gp51 modified silica substrate or unmodified silica surface
was treated by the same complement system protein containing solution for the same
Page 7
200 A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206
After exposure of antigen-antibody complex containing surfaces to complement sys-
tem containing solution we observed features larger in lateral dimension as well as in
height than either gp51 or antigen-antibody complexes. The image appears to show com-
plex formation, what was expected. The area density of the features is about 5-7 times
smaller if compared with the density of antigen-antibody complexes, and about 20-25
times smaller if compared with the density of immobilized gp51; it allows us to suppose
that some spatial factors are crucial for the formation of antigen-antibody-complement
complexes. We favor the notion that the relatively large antigen-antibody complexes may
exist in a wide range of orientations and conformations all of which are not optimal for
antigen-antibody-complement complex formation. Moreover, since the mixture of anti-
gp51 antibodies is polyclonal, several antibodies can bind respectively to different epitopes
of gp51 and such complex might be inconvenient for the formation of antigen-antibody-
complement components complex. Tapping mode phase images (Fig. 1E) clearly show
at least two distinct populations of features with different rigidity that are forming com-
plement system protein complex/complexes. This fact illustrates that different proteins
in the complement system posses different rigidity and might be distinguishable based on
this property by AFM phase imaging.
4 Conclusions and future developments
Our experiments demonstrate that AFM is usable for observing biological molecular pro-
cesses like antigen-antibody docking and even for the formation of larger biological struc-
tures. However, from the AFM data presented in this study it is still not possible to clearly
identify separate proteins, since the detection was performed in at least a partially dried
state and the protein complex appeared in an almost globular shape.
The results presented encourage a detailed AFM study of complement assembly by
application of purified complement system proteins.
This work was financially supported by Lithuanian State Science and Studies Foundation
project number C 03047, and COST action D33.
[1]D. Fotiadis, S. Scheuring, S.A. M¨ uller, A. Engel and D.J. M¨ uller: "Imaging and
manipulation of biological structures with the AFM", Micron, Vol. 33, (2002), pp.
S. Scheuring, F. Reiss-Husson, A. Engel, J.L. Rigaud and J.L. Ranck: "High-
resolution AFM topographs of Rubrivivax gelatinosus light-harvesting complex
LH2", Embo J., Vol. 20, (2001), pp. 3029–3035.
Page 8
A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206201
H.J. M¨ uller-Eberhard and P.A. Miescher (Eds.): Complement, Verlag, Berlin, 1985.
B. Walivaara, A. Askendal, I. Lundstrom and P. Tengvall: "Imaging of the early
events of classical complement activation using antibodies and atomic force mi-
croscopy", J. Coll. Interface. Sci., Vol. 187, (1997), pp. 121–127.
L. Mark, W.H. Lee, O.B. Spiller, D. Proctor, D.J. Blackbourn, B.O. Villoutreix and
A.M. Blom: "The Kaposi's sarcoma-associated herpesvirus complement control pro-
tein mimics human molecular mechanisms for inhibition of the complement system",
J. Biol. Chem., Vol. 279, (2004), pp. 45093–45101.
K.B.M. Reid: "The complement system: A major effector mechanism in humoral
immunity", Immunologist, Vol. 3, (1995), pp. 206–211.
N.R. Cooper: "Activation of the complement system", Contemp. Topics Mol. Im-
munol., Vol. 2, (1973), pp. 155–183.
J.S. Presanis, M. Kojima and R.B. Sim: "Biochemistry and genetics of mannan-
binding lectin (MBL)", Biochem. Soc. Trans., Vol. 31, (2003), pp. 748–752.
J.W Goers, V.N. Schumaker, M.M. Glovsky, J. Rebek and H.J. Muller-Eberhard:
"Complement activation by a univalent hapten-antibody complex", J. Biol. Chem.,
Vol. 250, (1975), pp. 4918–4925.
[10] J. Kuby: Immunology, W.H. Freeman and Company, New York, 1997.
[11] H.J. M¨ uller-Eberhard: "Molecular organization and function of the complement sys-
tem", Ann. Rev. Biochem., Vol. 57, (1988). pp. 321–347.
[12] A. Ramanaviciene, J. Acaite and A. Ramanavicius: "Circulating immune complexes
as indicators of environmental contamination", Envir. Toxicol., Vol. 19, (2004), pp.
[13] G. Goldberger, M.L. Thomas, B.F. Tack, J. Williams, H.R. Colten and G.N. Abra-
ham: "NH2-terminal structure and cleavage of guinea pig pro-cs, the precursor of the
third complement componen", J. Biol. Chem., Vol. 256, (1981), pp. 12617–12619.
[14] J. Bramham, C.T. Thai, D.C. Soares, D. Uhrin, R.T. Ogata and P.N. Barlow: "Func-
tional insights from the structure of the multifunctional C345C domain of C5 of
complement", J. Biol. Chem., Vol. 280, (2005), pp. 10636–10645.
[15] K.D. Caldwell, T. Sandberg, J. Hellstrom, P. Tengvall and J. Andersson: "Mucin ad-
sorption to polymeric surfaces: kinetics, quantification and reduction of complement
activation", Abstracts of papers of the American chemical society, Vol. 225, (2003),
pp. U712–U712.
[16] J. Wettero, A. Askendal, T. Bengtsson and P. Tengvall: "On the binding of comple-
ment to solid artificial surfaces in vitro", Biomaterials, Vol. 23, (2002), 981–991.
[17] M.J. Corey, R.J. Kinders, C.M. Poduje, C.L. Bruce, H. Rowley, L.G. Brown, G.M.
Hass and R.L. Vessella: "Mechanistic studies of the effects of anti-factor H antibodies
on complement-mediated lysis", J. Biol. Chem., Vol. 275, (2000), pp. 12917–12925.
[18] D.V. Rozanov, A.Y. Savinov, V.S. Golubkov, T.I. Postnova, A. Remacle, S. Tomlin-
son and A.Y. Strongin: "Cellular membrane type-1 matrix metalloproteinase (MT1-
MMP) cleaves C3b, an essential component of the complement system", J. Biol.
Chem., Vol. 279, (2004), pp.46551–46557.
Page 9
202 A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206
[19] D. Fotiadis, P. Qian, A. Philippsen, P.A. Bullough, A. Engel and C.N. Hunter:
"Structural analysis of the reaction center light-harvesting complex I photosynthetic
core complex of Rhodospirillum rubrum using atomic force microscopy", J. Biol.
Chem., Vol. 279, (2004), pp. 2063–2068.
[20] N. Fang and V. Chan: "Chitosan-induced restructuration of a mica-supported phos-
pholipid bilayer: An atomic force microscopy study", Biomacromolecules, Vol. 4,
(2003), pp.1596–1604.
[21] N. Bhasin, P. Carl, S. Harper, G. Feng, H. Lu, D.W. Speicher and D.E. Discher:
"Chemistry on a single protein, vascular cell adhesion molecule-1, during forced un-
folding", J. Biol. Chem., Vol. 279, (2004), pp. 45865–45874.
[22] K. Salchert, U. Streller, T. Pompe, N. Herold, M. Grimmer and C. Werner: "In vitro
reconstitution of fibrillar collagen type I assemblies at reactive polymer surfaces",
Biomacromolecules, Vol. 5, (2004), pp. 1340–1350.
[23] A. Hirai, Y. Tsujii, M. Tsuji and F. Horii: "AFM observation of band-like cellulose
assemblies produced by Acetobacter xylinum", Biomacromolecules, Vol. 5, (2004),
pp. 2079–2081.
[24] P. Vermette, H.J. Griesser, P. Kambouris and L. Meagher: "Characterization of
surface-immobilized layers of intact liposomes", Biomacromolecules, Vol. 5, (2004),
pp. 1496–1502.
[25] A. Rindlav-Westling and P. Gatenholm: "Surface composition and morphology of
starch, amylose, and amylopectin films", Biomacromolecules, Vol. 4, (2003), pp.
[26] A. Ramanaviciene and A. Ramanavicius: "Molecularly imprinted polypyrrole-based
synthetic receptor for direct detection of bovine leukemia virus glycoproteins",
Biosens. Bioelectron., Vol. 20, (2004), pp. 1076–1082.
[27] A. Ramanaviciene, G. Stalnionis and A. Ramanavicius: "Piezoelectric affinity sensor
for detection of bovine leukaemia", Biologija, Vol. 1, (2004), pp. 33–35.
[28] A. Ramanaviciene and A. Ramanavicius: "Application of polypyrrole for the creation
of immunosensors", Crit. Rev. Anal. Chem., Vol. 32, (2002), pp. 331–336.
[29] A. Ramanaviciene and A. Ramanavicius: "Pulsed amperometric detection of DNA
with an ssDNA/polypyrrole modified electrode", J. Anal. Bioanal. Chem., Vol. 379,
(2004), pp. 287–293.
[30] A.S. Tatham, N.H. Thomson, T.J. McMaster, A.D.L. Humphris, M.J. Miles and
P.R. Shewry: "Scanning Probe Microscopy Studies of Cereal Seed Storage Protein
Structures Scanning", Scanning, Vol. 21, (1999), pp. 293–298.
[31] S. Scheuring, D. Fotiadis, C. M¨ oller, S.A. M¨ uller, A. Engel and D.J. M¨ uller: "Single
proteins observed by atomic force microscopy", Single Mol., Vol. 2, (2001), pp. 59–67.
[32] M.C. Coen, R. Lehmann, P. Groening, M. Bielmann, C. Galli and L. Schlapbach:
"Adsorption and biactivity of protein A on silicon surfaces studied by AFM and
XPS", J. Colloid Interface Sci., Vol. 233, (2001), pp. 180–189.
[33] A. Ramanaviciene, J. Acaite and A. Ramanavicius: "Prevalence of viral infections in
ecologically different districts and a new electrochemical immunoassay", Acta Med.
Page 10
A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206 203
Lituanica, Vol. 8, (2001), pp. 224–229.
[34] O.H. Willemsen, M.M. Snel, K.O. van der Werf, B.G. de Grooth, J. Greve, P. Hin-
terdorfer, H.J. Gruber, H. Schindler, Y. van Kooyk and C.G Figdor: "Simultaneous
height and adhesion imaging of antibody-antigen interactions by atomic force mi-
croscopy", Biophys. J., Vol. 75, (1998), pp. 2220–2228.
[35] P.C. Zhang, C. Bai, P.K. Ho, Y. Dai and Y.S. Wu: "Observing interactions between
the IgG antigen and anti-IgG antibody with AFM", IEEE Eng. Med. Biol. Mag.,
Vol. 16, (1997), pp. 42–46.
[36] Q. Weiping, X. Bin, W. Lei, W. Chunxiao, Y. Danfeng, Y. Fang, Y. Chunwei and W.
Yu: "Controlled site-directed assembly of antibodies by their oligosaccharide moieties
onto APTES derivatized surfaces", J. Colloid Interface Sci., Vol. 214, (1999), pp. 16–
[37] U. Dammer, M. Hegner, D. Anselmetti, P. Wagner, D. Dreier, W. Huber and H.J.
G¨ untherodt: "Specific antigen/antibody interactions measured by force microscopy",
Biophys. J., Vol. 70, (1996), pp. 2437–2441.
[38] C.J. Roberts, M.C. Davies, S.J. Tendler, P.M. Williams, J. Davies, A.C. Dawles, G.D.
Yearwood and J.C. Edwards: "The discrimination of IgM and IgG type antibodies
and Fab' and F(ab)2 antibody fragments on an industrial substrate using scannng
force micrscopy", Ultramicroscopy, Vol. 62, (1996), pp.149–155.
[39] Y. Dong and C. Shannon: "Heterogeneous immunosensing using antigen and anti-
body monolayers on gold surfaces with electrochemical and scanning probe detec-
tion", Anal. Chem., Vol. 72, (2000), pp. 2371–2376.
Page 11
204 A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206
Fig. 1 Tapping mode AFM images of (A) silanized SiO2and the same surface consecu-
tively treated with: (B) gp51 for 2 h; (C) anti-gp51 for 30 min; (D) active complement
system proteins for 3 min; (E) active complement system proteins for 60 min. Images
covers area of 5000 × 5000 nm.
Page 12
A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206205
Height, [nm]
Height, [nm]
Height, [nm]
Height, [nm]
Height, [nm]
Fig. 2 Frequency diagrams of tapping mode of corresponding AFM images presented in
Fig. 1. of (A) silanized SiO2and the same surface consecutively treated with: (B) gp51
for 2 h; (C) anti-gp51 for 30 min; (D) active complement system proteins for 3 min; (E)
active complement system proteins for 60 min. Images covers area of 5000 × 5000 nm.
Page 13
206 A. Ramanaviciene et al. / Central European Journal of Chemistry 4(1) 2006 194–206
Fig. 3 Height-width study of typical features observed by tapping Mode AFM image of
silanized SiO2surface consecutively treated with: (A) gp51 for 2 h; (B) anti-gp51 for 30
min; (C) active complement system proteins for 3 min; (D) active complement system
proteins for 60 min. Images cover area of 1000 × 1000 nm. Height profiles are shown in
the lower panels for the black lines in the corresponding image; (E) control experiment
silanized SiO2treated by gp51 for 2 h and BLV not infected bovine blood serum (not
containing anti-gp51) for 30 min.


text:
Browse these popular birthday party categories:
Sort by:
per page
- 29 items
Sort by:
per page
- 29 items
Related Products Personalized Kids Birthday Candy Box
The secret to an amazing birthday party favor? It's in the box! These cute and colorful candy boxes come in ...
Personalized Kids Birthday Mini Candy Bar Wrapper
Add more sweetness to your birthday party sweets with these cute and colorful candy bar wrappers! These personalized candy bar ...
Personalized Cocktail Mix - Birthday Party Favors
As a kid, attending a birthday party meant going home with a goodie bag filled with candy and small toys. ...
Personalized Mini Glass Kids Birthday Candy Jar
Your little birthday party guests will be delighted when you tell them they can take these cute candy jars home! ...


text:
Even when they have nothing else, aspiring artists have a dream that motivates them to succeed—whether it's superstardom or simply earning enough to pay next month's rent. XV, however, has a lengthier To Do List than most, as he explains on this unreleased cut. Once he's put together a strong discography, he plans to retire while still in the "Top 5 Rappers" list, move to a more pleasant part of the map, and get his own The Bachelor-style reality show on VH1 (XV of Love?—well, that's still a ways away). In his guest bars, fellow up-and-comer TiRon describes a similarly elaborate fantasy; his dream involves him kicking back with one of the finest ladies around, eating Porterhouse steaks in his penthouse suite. "Planning ahead" is an understatement to describe these emcees' lofty goals, but it's hard not to admire their initiative—and, with the support of fans in the Booth and beyond, their dreams may well become reality.
Written by Burmy on 08/12/09
Submit your Rating
Square Bound Music/Warner Bros.
First DJ Booth Appearance:
"U Got It ft. Lil' Wayne" (2008)
You Might Like...
blog comments powered by DisqusTRENDING NOW
- Fast Food Music: How Our Hunger for More is Killing Hip-Hop
- Rihanna & Kanye's "FourFiveSeconds" is a Blue Collar Anthem
- What If Drake Didn't Sign To Young Money?
- Digging Up Your Favorite Rapper's Hidden Internet Gems
- The Liberation of Lupe Fiasco on "Tetsuo & Youth"
- No Money, No Family: Iggy Azalea's Insane Coming to America Story
- A Very Serious Lyrical Analysis of Lil Wayne's "Sorry 4 The Wait 2"
- 2014 Best of the Booth Award Winners (The Complete List)
- Who Was the Worst Rapper of 2014?
- Your Favorite Indie Rapper is Secretly Signed to a Major Label


text:
AuthorsDen.com Join (Free!) | Login
SIGNED BOOKS AUTHORS eBOOKS new! BOOKS STORIES ARTICLES POETRY BLOGS NEWS EVENTS VIDEOS GOLD SUCCESS TESTIMONIALS
Featured Authors: Eugene Meyer, iJoe email@example.com, iEdward Patterson, iJeanne Miller, iGracie McKeever, iDavid Gelber, iSky Purington, i
· Become a Fan · Contact me · Sponsor Me! · Articles · Poetry · News · Events · Stories · Blog · 115 Titles · 523 Reviews · Share with Friends! · Member Since: Aug, 2010 Chip Bergeron, click here to update your pages on AuthorsDen.
Featured Book A stranger's wonderful chronicles, Book 2 by Antoine Raphael
Share Save
Strafford Notes, December 14, 2012 Wednesday, December 14, 2011 12:55:00 PM
by Chip Bergeron
Dec. 1 4
2nd Wednesday of the month – 6:30 – 8:30pm – Water Street Bookstore, Exeter – Features followed by open mike (778-9731 (bookstore) or John Ferguson, JFerg315.aol.com)
Still -On
Dec .17. Jaffrey NH Audio Graffiti Poetry Series (Third Saturday of every month). Pot luck at 7pm, poetry open mic at 8pm, followed by a feature poet. $3 at the door MindFull Books 29 Main Street Jaffrey NH Audio Graffiti Poetry Series
- This month, Audio Graffiti is shaking things up. In addition to a poetry open mic and this month's feature, Hannah Galvin, there will also be a Christmas Present Slam. Any poet who wants to slam will get a Christmas present, but the winner of the slam will get to choose from everyone's present at the end. It's going to be a Yankee Swap, poetry-slam style!
December 14
John Van [Jean Charlier de] Gerson 1363- French theologist During the papal schism of 1378_1414, he attended the councils of Pisa (1409) and Constance (1414_18). He spent his last years in a monastery at Lyons teaching children, composing hymns and writing books on Christian mysticism.
http://www.novelguide.com/a/discover/ewb_06/ewb_06_02448.html Nostradamus [Michel de Nostre-Dam] 1503 -66 French astrologist/prophet
http://www.kirjasto.sci.fi/nostrada.htm Tycho Brahe 1546 -1601 Knudstrup Denmark, astronomer (Golden nose)
http://www.hps.cam.ac.uk/starry/tycho.html Juan N Gallego 1777 – Jan 9 1853 Spanish poet/interpreter (El dos the mayo)
Charles Wolfe (1791-1823 ) Irish poet
Frances Ridley Havergal, 1836- June 3, 1879 English devotional writer- authored such hymns as "Take My Life and Let It Be," "Who is on the Lord's Side?" and "I Gave My Life for Thee."
http://www.wholesomewords.org/biography/biorphavergal.html William Wells Jr. 1837 -1892 businessman, politician, and general in the Union Army [Brevet Major General (Union volunteers)] during the American Civil War ; Medal of Honor /the Battle of Gettysburg.
http://www.vermonthistory.org/journal/73/06_Cross.pdf Salvador Diaz Miron 1853 – June 12, 1928) Mexican poet (Los Cien Mejores Poemas)
http://www.poemhunter.com/salvador-diaz-miron/poems/ George VI 1895 - 6 February 1952 king of England (1936-52)
Paul Éluard born Eugène Émile Paul Grindel 1895-1952 French communist/poet/resistance fighter (Le Phénix) ;a founder of Surrealism
A Single Smile
A single smile disputes Each star with the gathering night A single smile for us both And the blue of your joyful eyes Against the mass of night Finding its flame in my eyes I have seen by needing to know The deep night create the day With no change in our appearance.
James Harold "Jimmy" Doolittle, USAF (1896 – September 27, 1993) American aviation pioneer. Doolittle served as a brigadier general, major general and lieutenant general in the United States Army Air Forces during the Second World War. He earned the Medal of Honor (Raid on Tokyo 1942)
http://www.airpower.au.af.mil/airchronicles/cc/doo.html Margaret Chase Smith 1897 - May 29, 1995 (Representative/Senator-R-ME)
Morey Amsterdam 1908 – 96 Chicago IL, comedian (Buddy-Dick Van Dyke Show)
http://www.imdb.com/name/nm0025440/ Spike Jones 1911 - May 1, 1965 Long Beach CA, composer (Cocktails for Two) http://www.spikejones.com/ Tove (Irma Margit) Ditlevsen (1917-1976) Danish author
Shirley Jackson (1919-August 8, 1965 ), U.S. short story writer - wrote 'The Lottery'
http://www.imdb.com/find?q=%27The+Lottery%27+++&s=all Raymond R. Patterson 1929-April 5,2001 , African-American poet, writer, and professor.
Carolyn M[arie] Rodgers (1945-2010 )Chicago native and poet / the black arts movement
People die from loneliness. Life becomes an incurable disease, a job, an excuse-an operation of sloppy dissections.
There is a constipation of the heart, a diarrhea of need. Be- ing is instinct, the body a machine-the mind a lever or
the body the lever, the mind the machine; in either case, operating and driving on. And skin tightening up bone until you mouth at the misery
and bargain with the ache. This is not to say I am giving up, even though life has pumped me up with the pain. The rules are the I am the stray one...
Stanley Crouch (1945- ) California-born African-American essayist
http://www.aaregistry.org/historic_events/view/stanley-crouch-critic-columnist-novelist-who-speaks-out Patty Duke [Anna Marie] 1946 - Elmhurst NY, actress (Miracle Worker)
http://www.officialpattyduke.com/ Michael Ovitz 1946 - president (Walt Disney Company) http://michaelovitz.com/ Bill Buckner 1949 - California, 1st baseman, error cost Red Sox 1986 world series
Amy Hempel (1951- ) American short story writer, journalist, and university professor at Brooklyn College.
http://www.theparisreview.org/interviews/227/the-art-of-fiction-no-176-amy-hempel Alan Dennis Kulwicki (1954 – April 1, 1993), nicknamed "Special K" and the "Polish Prince", American NASCAR Winston Cup Series (now Sprint Cup Series) racecar driver. 1992 NASCAR Winston Cup championship
San Juan de la Cruz [de Yepes] , (24 June 1542 –1591), Spanish Carmelet/poet/saint
George Washington , Feb 22 ,1732 -1799 1st President USA (1789-97
Prince Albert of Saxe-Coburg and Gotha (Prince Consort; 26 August 1819 – 14 December 1861) husband of Queen Victoria of Great Britain and Ireland.
Jean Louis Rodolphe Agassiz , May 28,1807 -1873 Swiss/American geologist/paleo-biologist)
http://www.ucmp.berkeley.edu/history/agassiz.html Stanley Grauman Weinbaum (April 4, 1902 –1935) American science fiction author (Martian Odyssey)
http://www.gutenberg.org/ebooks/search.html/?default_prefix=author_id&sort_order=downloads&query=25246 Josef Kramer , 1906-1945 known as "beast of Belsen", & 10 others hanged for crimes committed at the Belsen and Oswiecim Nazi concentration camps http://www.jewishvirtuallibrary.org/jsource/biography/kramer.html Dinah Washington , Aug 8 ,1929-1963African American Blues singer
Walter Lippman (09/23/1889 –1974) US writer - journalist (One of Dynasty),
http://www.spartacus.schoolnet.co.uk/USAlippmann.htm Vicente Aleixandre ,1898 - 1984 Spanish poet (Historia del corazón) Nobel Prize for Literature in 1977.
Lover What I do not want is to give you the words of day dreams. Not to spread the image with my lips on your face, nor with my kiss. I take the tip of your finger with pink nail, for my gesture, and, in this manner of airs, I give it back to you. From the grace and the lightsomeness of your pillow. And the heat of your exotic eyes. And the light of your secret breasts. Like the moon in the spring a window gives us yellow light, and a heart beat seems to flow back from you to me. It's not that. Nor will it be. Your true sense has already given me the peace, the beautiful secret, the charming dimple, the lovely corner of your mouth and the weary morning.
http://tanzankopra.blogspot.com/2006/03/vicente-aleixandre-mexico.html Roger Eugene Maris , September 10, 1934 -1985 homerun hitter (61 in 61, New York Yankees)
http://www.baseball-reference.com/players/m/marisro01.shtml Andrei D Sakharov , (05/21/1921 –1989) Soviet Physicist/Dissident/1975 Nobel peace prize winner
Friedrich Durrenmatt (01/05/1921 –1990) Swiss writer
Myrna Loy [Williams] , August 2, 1905 - 1993 actress (Thin Man, Vanity Fair)
http://www.imdb.com/name/nm0001485/ Owen Barfield , (9 November 1898 –1997) was a British philosopher, author, poet, and critic. /philosopher of language
Trevanian Rodney William Whitaker (12 June 1931 -2005) American film scholar and write
Bonus Poem:
On Being Told That There Is No Rhyme For The Word "Limerick"
After I heard Harry's limerick, I found that my mind set to simmer. "Rick, " I said to myself, "One might write it itself, " But I answered, "You're chances are slimmer, Rick! " So I can't find a good rhyme for Limerick. Told myself, " You are just a beginner, Rick And though there's no reason You can't write something decent, I fancy your chances get grimmer, Rick! " He said that it might rhyme with turmeric But I thought, "You're not that fast a learner, Rick: Ditch your thoughts wasting time Finding suitable rhyme; Let your plans, man, stand on the back burner, Rick." But the cogwheels had started to turn a bit As I guessed I had started to learn a bit And I might find a line That rhymes Limerick fine And might win me a prize, so I earn a bit. But I thought, "Though there was just a glimmer, Rick. You will think till you're needing a zimmer, Rick, Since the muse isn't kind, I am sure you'll will find Not a word that will rhyme fine with Limerick." -C Richard Miles
Featured Book Kuki Book One - The End by Lumix Luminous
Featured Book The Immigrants' Daughter by Mary Terzian
Featured Authors | New to AuthorsDen? | Add AuthorsDen to your Site Share AD with your friends | Need Help? | About us


text:
Standard guitar tuning:EADGBENo capoIntro
|: Bm6 |C#7b9 |F♯m |N.C. :| REPEAT X4Verse 1
BmI run the nC♯7umbers through the F♯mfloor F♯7 BmHere's how it gC♯7oes, I crack the cF♯modes, I crack the cF♯7odes that end the warFig
E|---------------------------------------| B|---------------------------------------| G|---4---4--7--7----6---6---6--4--2------| D|---------------------------------------| A|---2---2--5--5----4---4---4--2--0------| E|---------------------------------------|I crack the codes that end the warFig
E|---------------------------------------| B|-----------------------------6--7------| G|---4---4--7--7----6---6---6------------| D|-----------------------------3--4------| A|---2---2--5--5----4---4---4------------| E|---------------------------------------|The Bmhour The F♯mhour The Bmhour The F♯mhourVerse 2
Fig.1 and Fig.2 throughout BmI pushed a C♯7note under yourF♯m door F♯7 BmHere's how it gC♯7oes, things come to F♯mblows, but we don't waF♯7nt this anymore Bm C♯7 F♯m No we don't want thF♯7is anymore Bm C♯7 F♯m We don't want thF♯7is anymore Bm C♯7 F♯m I crack the codes you end the warA C♯ D F♯
AI hear the clockwork in your C♯core DTime strips the gears till you foF♯rget what they were for AI push the numbers through your C♯pores DI crack the codes I crack the cF♯odes that end the war F♯7MHow's A♭my C♯living F♯7MYou A♭can C♯call F♯7MEncryptedA♭ C♯numbers on F♯7MBathroom A♭ C♯stalls, there's F♯7MSomethingA♭ C♯burning, it F♯7MCasts A♭a C♯pall, it's F♯7MMelting A♭ nuC♯mbers right F♯7MOff A♭the C♯wallInterlude
Bm F♯
BmI run the numbers through the F♯floor BmHere's how it goes, I crack the F♯codes, I crack the codes, you end The Bmwar F♯Outro
Repeat and fade The Bmhour, the hour, the hour, the hour, The F♯hour, the hour, the hour, the hour...
Terms and Privacy | API | Just Added Tabs | Tags | How to Read Guitar Tab | Music Theory | Contact/Support
© 2015, Songsterr.com.
© 2015, Songsterr.com.


text:
View jewish Singles in South Weymouth
By clicking continue, I agree to receive transactional and promotional emails from Match.com. I understand that I am free to withdraw consent at any time.
By using our site, you agree to the Match.com Terms of Use.
The Bay State of Massachusetts has thousands of singles looking for love. Find your match today! We prove that an online dating personals site can really lead to a long-lasting sincere relationship. Online dating in South Weymouth,Massachusetts has never been easier. Back in the saddle again? Start dating online in [$CITY]. Select your dream date from thousands of South Weymouth online personal ads with photos.
Abington, Accord, Assinippi, Avon, Braintree, Brockton, Canton, Cohasset, Dorchester, Dorchester Center, East Bridgewater, East Weymouth, Greenbush, Hanover, Hanson, Hingham, Holbrook, Hull, Hyde Park, Mattapan, Milton, Milton Village, Minot, North Marshfield, North Pembroke, North Quincy, North Scituate, North Weymouth, Norwell, Quincy, Randolph, Readville, Rockland, Scituate, Squantum, Stonehill College, Stoughton, Weymouth, Whitman, Wollaston view all states »
Asian , Black , Catholic , Christian , Divorced , Free Online Dating, Gay Singles, Gay Dating, Jewish , Lesbian Singles, Single Parents, view all communities »


text:
Dr. Matthew A. Ciesielski, DO
Family Medicine, Bronson Family Medicine
- 3300 W Centre Ave
Portage, MI 49024 - 1 Office Location
- (269) 324-8950
Dr. Matthew A. Ciesielski graduated from the University of New England College of Osteopathic Medicine in 1998. He works in Portage, MI and specializes in Family Medicine. Dr. Ciesielski is affiliated with Bronson Methodist Hospital. He speaks English and Spanish.
Gender: Male
Years in Practice: 17 Years
Languages Spoken: English, Spanish
Medical School: University of New England College of Osteopathic Medicine
Graduation Date: 1998
Hospital Affiliations: Bronson Methodist Hospital
Specialties: Family Medicine