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Acetate is a commonly used substrate for biosynthesis while monochloroacetate is a structurally similar compound but toxic and inhibits cell metabolism by blocking the citric acid cycle. In Burkholderia species MBA4 haloacetate was utilized as a carbon and energy source for growth. The degradation of haloacid was mediated by the production of an inducible dehalogenase. Recent studies have identified the presence of a concomitantly induced haloacetate-uptake activity in MBA4. This uptake activity has also been found to transport acetate. Since acetate transporters are commonly found in bacteria it is likely that haloacetate was transported by such a system in MBA4.
The haloacetate-uptake activity of MBA4 was found to be induced by monochloroacetate (MCA) and monobromoacetate (MBA). While the acetate-uptake activity was also induced by MCA and MBA, other alkanoates: acetate, propionate and 2-monochloropropionate (2MCPA) were also inducers. Competing solute analysis showed that acetate and propionate interrupted the acetate- and MCA- induced acetate-uptake activities. While MCA, MBA, 2MCPA, and butyrate have no effect on acetate uptake they could significantly quenched the MCA-induced MCA-uptake activity. Transmembrane electrochemical potential was shown to be a driving force for both acetate- and MCA- transport systems.
Here we showed that acetate- and MCA- uptake in Burkholderia species MBA4 are two transport systems that have different induction patterns and substrate specificities. It is envisaged that the shapes and the three dimensional structures of the solutes determine their recognition or exclusion by the two transport systems.
Burkholderia species MBA4 is a Gram-negative bacterium enriched from soil using monobromoacetate (MBA) as the sole carbon and energy source for growth. MBA4 can also utilize other haloacids such as monochloroacetate (MCA), 2-monochloropropionate (2MCPA) and 2-monobromopropionate (2MBPA) [1]. Since haloacids are environmental pollutants [2-5] and are potentially hazardous for many living organisms [6-8], it is crucial to identify and characterize bacteria that can degrade these alkanoates. The ability for MBA4 to utilize haloacids is conferred by a 2-haloacid dehalogenase Deh4a [1] which has been well characterized [9-11]. A haloacid permease gene, deh4p, which forms an operon with deh4a, was identified by means of chromosome walking [12]. The function of Deh4p was confirmed by heterologous expression in E. coli[13], and its topology determined with a PhoA-LacZ dual reporters system [14]. Further characterization of MBA4 showed that a Deh4p paralog, designated as Dehp2, is also playing a role in MCA uptake. The functional role of Dehp2 was confirmed by gene disruption and heterologous expression in E. coli. Single disruptants of deh4p or dehp2 were found to have 30% less of MCA-uptake activity. Moreover, cells with a disrupted deh4p gene have an enhanced expression in dehp2 and vice versa. It looks like Deh4p has a higher affinity for MCA while Dehp2 prefers chloropropionate. When a deh4p‒ dehp2‒ double disruptant was constructed, the cells still retain 36% of MCA-uptake activity. It was concluded that a robust system is present for haloacid uptake in MBA4 [15].
In the process of characterizing the MCA-uptake activity of MBA4, it was found that acetate was also recognized by the MCA-inducible uptake system [12,15]. Since acetate and MCA are structurally similar, it is reasonable to speculate that MCA was transported by an acetate-transport system. It has been reported that acetate could freely diffuse across the cell membrane in an un-disassociated form (acetic acid) [16]. However, in growth conditions with a neutral pH where acetate is mainly in a disassociated form, a specific transport system is needed. There are reports leading to the identification of acetate permeases in many bacterial species, including ActP in Gram-negative E. coli [17] and MctC in Gram-positive Corynebacterium glutamicum [18]. As MBA4 can grow on acetate, it is likely that an acetate-transport system is also present. Whether this acetate-transport system is playing a role in MCA uptake is important to the understanding of the MCA-uptake system in MBA4.
In this study, we analyzed the induction patterns of the acetate- and MCA-uptake systems and determined the substrate specificities of the two systems in cells grown in various substrates. We demonstrated that there are distinct acetate- and MCA- transport system in MBA4. Nonetheless, both systems were sensitive to carbonyl cyanide m-chlorophenyl hydrazone indicating that transmembrane electrochemical potential is a driving force for both systems.
The induction patterns of acetate- and MCA- transport systems are different
Previous analysis showed that MCA-uptake activity of MBA4 was detected only in MCA- and not in acetate-grown cells [12]. This indicated that the MCA-uptake activity was induced by MCA and not by acetate. It was also shown that MCA-grown cells possess acetate-uptake activity [12]. To check whether a distinct acetate-transport system is present in MBA4, acetate-uptake assays were carried out for pyruvate-, acetate-, and MCA-grown cells. The results showed that pyruvate-grown cells had no detectable activity, while both acetate- and MCA-grown cells had significant acetate-uptake activities (Figure 1). Acetate-grown cells had an acetate-uptake rate of 111.27 nmol (mg protein)-1 min-1 for the first 8 min, and MCA-grown cells had a rate of 59.20 nmol (mg protein)-1 min-1. This indicated that acetate was not entering the cells passively, and there is an inducible acetate-transport system in MBA4.
Figure 1. Acetate-uptake activity of MBA4. MBA4 was grown in minimal medium containing pyruvate (squares), acetate (circles), or MCA (triangles). Uptake of 50 μM of [2-14C]acetate was assayed by a filtration method for a period of 8 min. Data shown are the means of three independent experiments, and the error bars represent the standard deviations.
In order to characterize the inducible acetate-uptake system, MBA4 cells were grown in various carbon sources and their relative acetate-uptake activities, using acetate-grown cells as the standard, determined. Figure 2A shows that propionate induced similar level of uptake activity while MCA, MBA and 2MCPA only induced around 50% of the standard. Butyrate and valerate induced less than 20% of activity. As a comparison, cells were also grown in similar substrates and their relative MCA-uptake activities determined. Figure 2B shows that MBA induced comparable MCA-uptake activity as MCA but 2MCPA only induced about 20% of activity. The inductions conferred by acetate, propionate, butyrate, and valerate were rather minimal and only represent a mere 10% or less. As the MCA-uptake activity was induced significantly only by monohaloacetate while the acetate-uptake activity induced by acetate, haloacetate, propionate and 2MCPA, the induction patterns of the two transport systems appear to be different.
Figure 2. Relative acetate- and MCA- uptake activities of MBA4 grown in various substrates. MBA4 was grown in minimal medium containing acetate, MCA, MBA, propionate, 2MCPA, butyrate, or valerate. Uptakes of 50 μM of [2-14C]acetate (A) or [2-14C]MCA (B) were assayed for a period of 1 min. Data shown are the means of three independent experiments, and the error bars represent the standard deviations. (A) The acetate-uptake rate of acetate-grown cells was set as 100%, and acetate-uptake rates of cells grown in other substrates were determined and shown as percentages. (B) The MCA-uptake rate of MCA-grown cells was set as 100%, and MCA-uptake rates of cells grown in other substrates were determined and shown as percentages.
Acetate- and MCA- transport systems have different substrate specificities
In order to conclude that the transports of acetate and MCA were executed by different systems, competing solute analysis was used to deduce the substrate specificities of the induced acetate- and the MCA- transport systems in MBA4. Acetate uptakes were determined for both acetate- and MCA-grown cells. MCA uptakes were determined only for MCA-grown cells because acetate-grown cells have no MCA-uptake activity. Competing solutes that exhibit structural similarity to acetate or propionate were selected.
Acetate uptake of acetate-grown cells was significantly inhibited by acetate and propionate, with an inhibition of 91% and 90%, respectively (Figure 3A). When MCA-grown cells were used, a similar pattern was observed for acetate uptake. Only acetate and propionate served as effective inhibitors (Figure 3B). When MCA-grown cells were used for MCA-uptake assays, acetate, MCA, MBA, propionate, 2MCPA and butyrate acted as efficient inhibitors. In addition, glycolate, lactate, and pyruvate also had moderate inhibitory effects on MCA uptake as previously reported [12] (Figure 3C). These results showed that the acetate-uptake activity was inhibited only by acetate and propionate while the MCA-transport system was inhibited by substrates that display a similar structure as haloacetate.
Figure 3. Inhibition of acetate- and MCA- uptake by other solutes. Uptakes of 50 μM of [2-14C] labelled acetate or MCA were determined in the presence of competing solutes. The assays were conducted for 1 min. Competing solutes were added to a final concentration of 0.5 mM. Competing solutes used were: ethanol, formate, glycolate, lactate, pyruvate, succinate, acetate, MCA, MBA, propionate, 2MCPA, butyrate, and valerate. Uptake rates without competitor were used as the controls. Data shown are the means of three independent experiments, and the error bars represent the standard deviations. (A) Acetate uptake of acetate-grown cells; (B) Acetate uptake of MCA-grown cells; (C) MCA uptake of MCA-grown cells.
Transmembrane electrochemical potential is a driving force for both acetate- and MCA- transport
During the characterization of the haloacid operon of MBA4, a protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), was shown to abolish the MCA-uptake activity of MBA4 (M. Yu, unpublished). The effect of CCCP on acetate uptake was duly investigated. Figure 4 shows that the inclusion of increasing amount of CCCP in uptake assays for acetate- and MCA-grown cells, the acetate-uptake rates decreased accordingly. The uptake activities were completely abolished when 25 μM of CCCP were supplemented in the reactions. As CCCP collapses the proton gradients across the cell membrane [19], acetate uptake in MBA4 is likely to be dependent on the transmembrane electrochemical potentials, a condition similar to that of MCA uptake.
Figure 4. Effect of CCCP on acetate uptake. MBA4 was grown in minimal medium containing acetate (squares) or MCA (circles). Uptakes of 50 μM of [2-14C]acetate were assayed in the presence of 0, 5, 10, 25, and 50 μM of CCCP for a period of 1 min. Data shown are the means of three independent experiments, and the error bars represent the standard deviations.
A limitation of employing CCCP is that it cannot discriminate between proton-coupled symport and Na+-coupled symport [17,20]. As it is difficult to remove sodium from the buffers completely and radioactive MCA and acetate were provided in the form of a sodium salt, the effect of pH on acetate- and MCA- uptake was examined with an aim to find out the possible involvement of proton(s). In acetate uptake of acetate-grown cells, the uptake rate decreased steadily as pH increased from 4 to 8 (Figure 5, squares). In acetate uptake of MCA-grown cells, the uptake rate increased slightly as pH increased from 4 to 5 and then dropped gradually as pH increases (Figure 5, circles). The uptake rates were much lower than that of acetate-grown cells in similar assay conditions. In MCA uptake of MCA-grown cells, the uptake rate increased slightly as pH increased from 4 to 6 and dropped swiftly from pH 7 to 8 (Figure 5, triangles). These results showed that acetate- and MCA- transport systems have different sensitivities to pH. Nonetheless, the involvement of proton(s) in acetate transport is noticeable.
Figure 5. Effect of pH on acetate- and MCA- uptake. MBA4 was grown in minimal medium containing acetate or MCA, harvested and resuspended in potassium phosphate buffers of various pH values. Uptakes of 50 μM of [2-14C] labelled acetate or MCA were assayed for a period of 1 min. Squares represent acetate uptake of acetate-grown cells, circles represent acetate uptake of MCA-grown cells, and triangles represent MCA uptake of MCA-grown cells. Data shown are the means of three independent experiments, and the error bars represent the standard deviations.
In this study, we demonstrated the presence of distinct acetate- and MCA- transport system in MBA4. This is supported by: (i) the observation that the inducible substrates for acetate- and MCA- uptake activity were different; (ii) the two transport systems have different competing solutes and (iii) a difference in dependency on pH for the two systems. The failure of pyruvate-grown cells to take up acetate suggested that the acetate-transport system in MBA4 was inducible. Both acetate and MCA were able to induce acetate-uptake activity although to a different level. Acetate permease MctC of Corynebacterium glutamicum is also inducible. MctC exhibits a high affinity for acetate and propionate and low affinity for pyruvate. In this case, the expression was higher in pyruvate- than in acetate-grown cells. As a result, both pyruvate- and acetate-grown cells showed comparable acetate-uptake activities [18]. In MBA4, no induction was observed for pyruvate while acetate and propionate were the best inducers for acetate uptake. Moreover, they were also the most favourable substrates. It is possible that acetate and propionate were transported by the same transport system but further confirmation is required as Candidatus Competibacter phosphatis appeared to have different transporters for the two solutes [21]. Another acetate permease, ActP of Rhodobacter capsulatus, was produced around 1.5 folds more in acetate- than in pyruvate-grown cells [22]. This indicated that the regulations of various acetate-transport systems in different bacteria are likely to be different and should be compared cautiously.
It is not surprising that MCA-grown cells could take up MCA and acetate because most transporters recognize more than one substrate. Acetate permease ActP of E. coli was able to transport acetate and glycolate [17]. Moreover, acetate and MCA are structurally similar molecules. The ability for MCA-grown cells to transport acetate can be explained by (1) the capability of the induced MCA-transport system to transport acetate; (2) the acetate-transport system was also induced by MCA; and (3) both (1) and (2). Without the identification of the individual permease involved in each of the transport system it is difficult to determine conclusively which the case is. The cloning and heterologous expression of Deh4p in E. coli demonstrated its function as a dehalogenase-associated MCA-transporter [13]. Similarly, the functional role of Dehp2 as a second MCA-transporter was also demonstrated [15]. Both Deh4p and Dehp2 were capable of recognizing acetate as a substrate. In order to elucidate that the MCA-uptake system, comprising Deh4p and Dehp2, is not the main transporter for acetate, a deh4p‒ dehp2‒ double mutant (strain Ins-4p-p2, [15]) was utilized. Figure 6A shows that the growth of Ins-4p-p2 on acetate was similar to that of wildtype MBA4, if not slightly better. The acetate-uptake rate of this acetate-grown mutant was also assayed and shown to be similar to that of wildtype (112.3 nmol (mg protein)-1 min-1 for mutant and 118.6 nmol (mg protein)-1 min-1 for wildtype, Figure 6B). This suggested that in the absence of the major players in MCA uptake the growth and uptake activity on acetate of the cell were not affected. This confirmed the presence of an independent acetate-transport system other than the MCA-uptake system.
Figure 6. Growth on and uptake of acetate of a deh4p– dehp2– double mutant. (A) Wildtype MBA4 (■) and deh4p– dehp2– double mutant (□) were grown in minimal medium containing acetate. Seed cultures were grown in LB– and sub-cultured into minimal medium containing acetate at 30°C. The optical densities of the cultures were determined at 600 nm (OD600) with a spectrophotometer. (B) Acetate-uptake rates of acetate-grown- wildtype and double mutant. Uptakes of 50 μM of [2-14C]acetate were assayed by a filtration method for a period of 2 min. Data shown are the means of three independent experiments, and the error bars represent the standard deviations.
The competing solute analyses show that acetate- and MCA-grown cells have similar inhibition pattern for acetate uptake. This suggested that the acetate-transport system was likely to be induced by MCA. The relatively lower acetate-uptake rate for MCA-grown cells suggested that MCA was a weaker inducer. This is consistent with the observation that acetate and propionate were the best inducers for acetate uptake. The competing solute analyses for MCA-grown cells show that the cells have different inhibition patterns for acetate- and MCA- uptake. The failure of MCA to inhibit the uptake of acetate suggested that the acetate-transport system was expressed and not involved in MCA transport. This is in agreement with the result that acetate-grown cells failed to transport MCA. The ability for acetate to inhibit the MCA-uptake activity of MCA-grown cells concluded that the MCA-uptake activity is different from the acetate-uptake system.
The effect of pH on the uptakes of acetate of acetate- and MCA-grown cells further demonstrates the presence of two systems. The uptake rates of acetate-grown cells decrease linearly with an increase in pH. This shows that proton plays an essential role in the acetate-uptake system. In this condition no MCA-uptake system was produced. When the cells were grown on MCA the rates of acetate uptake on different pH deviate from that of acetate-grown cells. The competing solute analysis demonstrated a similar pattern of inhibition on acetate uptake for acetate- and MCA-grown cells while the rate was much lower for the latter. It is most likely that the expression of the acetate-uptake system was lower in MCA-grown cells. In this case, the major transport system was that for MCA and which can also transport acetate. Since both acetate- and MCA- transport systems are proton dependent, the pH dependency of acetate uptake of MCA-grown cells was thus exhibiting a pattern different from that of acetate-grown cells and was displaying a hybrid pattern between acetate uptake of acetate-grown cells and MCA uptake of MCA-grown cells. Future experiments that assay the pH dependency of acetate uptake of MCA-grown Ins-4p-p2 double mutant could clarify the situation. However, the expressions of other transporters may be affected by the disruptions of deh4p and dehp2 [15] and could complicate the outcome. Moreover, when the gene responsible for the acetate-uptake system has been identified, it is necessary to measure its expression levels in medium containing acetate, MCA and other substrates in order to characterize the system fully.
The most distinct difference between the two transport systems is their substrate specificity. The failure of ethanol to inhibit acetate transport suggested that the carboxyl group is likely to be an important element. The lack of inhibition by formate implied that the presence of a second carbon is also essential. The failure of MCA, MBA, and 2MCPA to inhibit acetate transport indicated that the substituted halogen atom affected the effective recognition. The acetate-uptake ability of MBA4 was inhibited by propionate but not by butyrate. This is consistent with the acetate permease ActP of E. coli [17]. The failure of butyrate and valerate to act as a competing solute suggested that a molecule with more than three carbons would be less effective for the acetate-uptake system. In summary, MCA, MBA, 2MCPA, and butyrate could inhibit MCA- but not acetate- uptake of MBA4. A visual inspection of the structural models of these molecules (Figure 7) suggests that they are generally larger than acetate. Similarly, MCA, MBA, and propionate have a stronger inhibitory effect on MCA uptake than 2MCPA and butyrate. The failure of valerate to act as a competing solute further strengthens the notion that size is a determining factor. By means of comparing the structures of the competing solutes it may be possible to estimate the range of substrates recognized by various transport systems and provide valuable information on the functional property of the transporters.
Figure 7. Structural models of various competing solutes. The values of atomic radii, the skeletal formula and the space-filling models of acetic acid, MCA, MBA, propionic acid, 2MCPA, butyric acid, and valeric acid were obtained from ACD/ChemSketch (Advanced Chemistry Development, Inc.). The solutes were assumed to be in disassociated forms in PBS buffer (pH 7.4) used in this study.
The inducers for the acetate-uptake system are acetate, MCA, MBA, propionate, and 2MCPA, but only acetate and propionate are substrates. Similarly, the inducers for the MCA uptake system are MCA, MBA and to a lesser extend 2MCPA, while the substrates include the inducers, acetate and propionate. The inducer and the substrate are not necessarily the same. Although the acetate- and the MCA- transport systems have different induction patterns and substrate specificities, they do share certain similarity. The activities of both systems were abolished by CCCP, suggesting transmembrane electrochemical potential as a driving force. As CCCP could not discriminate between proton- and sodium-coupled symport, it was unclear which was/were involved in the transports. Previous studies of bacterial acetate-transport systems failed to give a uniformed conclusion. Although ActP of E. coli was assigned to the sodium:solute symporter family, no dependency on sodium was demonstrated [17]. While electrochemical proton potential was confirmed to be a driving force for MctC of Corynebacterium glutamicum [18], acetate uptake in Accumulibacter spp. was believed to be driven by proton motive force, and in Defluviicoccus spp. it was suggested to be powered by proton or sodium gradient or both [23]. An increased uptake of acetate for a change of pH from 8 to 4 affirmed the involvement of proton in acetate transport in MBA4. However, the involvement of sodium could not be ruled out and further confirmation is required.
The uptakes of acetate and MCA in Burkholderia species MBA4 were demonstrated to be manoeuvred by different transport systems. These systems have different induction patterns and substrate specificities. A driving force for both systems is transmembrane electrochemical potential, and proton is involved in acetate transport. A structural comparison of the competing solutes suggests that the size of the molecule is a determinant factor for recognition. Future work on identification and characterization of the transporter protein is required to understand the systems comprehensively.
Bacterial strains and culture conditions
Burkholderia species MBA4 and mutant Ins-4p-p2 were grown at 30°C in Luria Bertani medium without NaCl (LB–, 1% tryptone, 0.5% yeast extract) or in defined minimal medium [1] with 0.5 g carbon liter-1 of pyruvate, acetate, MCA, MBA, propionate, 2MCPA, butyrate, or valerate.
Transport assays
MBA4 was cultured in minimal medium with pyruvate, acetate, MCA, MBA, propionate, 2MCPA, butyrate, or valerate to late logarithmic phase, with an optical density value (OD600) of 1.0-1.2, 0.9-1.1, 0.5-0.7, 0.7-0.9, 0.9-1.1, 0.1-0.2, 0.9-1.1 or 0.9-1.1, respectively. Cells were harvested by centrifugation, washed twice with phosphate buffered saline (PBS, Fluka), and adjusted to an OD600 of around 0.4. For standard transport assays, 30 μl of [2-14C]MCA (Sigma-Aldrich, diluted to 0.25 mM in PBS) or [2-14C]acetate (Sigma-Aldrich, diluted to 0.25 mM in PBS) were added to 120 μl of prepared cells, mixed, and 30 μl samples were taken at various time points. Filtration and washing of cells, determinations of total protein and trapped [2-14C]MCA or [2-14C]acetate were carried out as previously described [12].
To determine the substrate specificity, diluted [2-14C]MCA or [2-14C]acetate was mixed with 10× competing solutes in PBS before adding to the prepared cells. Percent relative uptake was calculated as (Uptake rate with competing solute/Uptake rate without competing solute) × 100%. The competing solutes included: ethanol; one-carbon monocarboxylate formate; two-carbon glycolate, acetate, MCA and MBA; three-carbon propionate, lactate, pyruvate and 2MCPA; four-carbon butyrate, five-carbon valerate; and four-carbon dicarboxylate succinate. The skeletal formulas and space-filling models of acetic acid, MCA, MBA, propionic acid, 2MCPA, butyric acid, and valeric acid were drawn with ACD/ChemSketch (Advanced Chemistry Development, Inc.).
To study the effect of protonophore on uptake assay, appropriate amounts of carbonyl cyanide m-chlorophenyl hydrazone (CCCP) were mixed with prepared cells to a final concentration of 0, 5, 10, 25, and 50 μM for 30 min before transport assays were conducted.
To determine the effect of pH on transport systems, 100 mM potassium phosphate buffers of different pH values (4 to 8) were used to resuspend the bacterial cells and for diluting [2-14C]MCA and [2-14C]acetate for uptake assays.
MCA: Monochloroacetate; MBA: Monobromoacetate; 2MCPA: 2-Monochloropropionate; 2MBPA: 2-Monobromopropionate; CCCP: Carbonyl Cyanide M-Chlorophenyl Hydrazone; PBS: Phosphate Buffered Saline.
Competing interests
The authors declare that they have no competing interests.
Authors contributions
XS and KFK designed and carried out the studies and drafted the manuscript. JSHT conceived of the study, participated in the design and coordination of the study and drafted the manuscript. All authors read and approved the final manuscript.
We thank WK Yip for the use of a scintillation counter. XS thanks the University of Hong Kong for a studentship. This work was partially supported by the University Seed Funding Programme for Basic Research 2011.
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Operation Detachment, the invasion of Iwo Jima, on 19 February 1945 was the first campaign on Japanese soil and it resulted in some of the fiercest fighting of the Pacific campaign. United States Marines supported by the US Navy and Air Force fought the Japanese both over and underground on the island of volcanic ash, in a battle which was immortalised by the raising of the Stars and Stripes above Mount Suribachi. It was a battle that the Japanese could not win but… (more)
Publisher: Spellmount (November 30, 2011)
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Page count: 160 pages
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休闲活动 Chiang Mai
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Since 2002, CMRCA has provided award-winning rock climbing guide services and courses in addition to corporate team-building and youth experiential education programs in stunningly beautiful Northern ... 更详细信息
Standard Tour Company was established in the year of 1990. The headquarter located right in the center of Chiang Mai business area. We provide professional turn-key services in all levels of travel bu... 更详细信息


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Sun, Aug 31, 14 at 16:37
We are having a 18x36 free form kidney pool installed with a rough in for a water feature (won't be installed for another few years). We are wondering what type of pump to install, a flopro vs or 2 single hp pumps. The builder is suggesting two single hp pumps but I am inclined to think that a single vs pump would suffice. Any suggestions or reccomendations would be appreciated. |
Follow-Up Postings:
I just had a pool built and have two single speed pumps. I have four fire/water bowls and to have the bowls running I would have to run the VS pump on high anyways. My pool builder was not a big fan of the VS pumps though they seem to be very popular around this area of Texas. I went with his suggestion mostly because of my previous experience with pool pumps. I just didn't see them ever paying for themselves before needing replaced. |
- Posted by muddy_water (My Page) on Mon, Sep 1, 14 at 20:44
I would go with a VS pump because you should be able to lower the speed when not running the feature and really dial it in for flow when you want too. Intelliflow from Pentair. |
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text: Extra Scoop: Robin Thicke's First Words Since Split from Paula Patton February 26, 2014 | Celebrity News Share Tweet Pin It Comment Robin Thicke's First Words Since Split from Paula Patton Singer Robin Thicke posted a thank-you on his website, his first statement since he and Paula Patton announced their split. Full story: L.A. Times Jessica Alba Calls Acting 'Her Drug of Choice' The actress is Nylon magazine's March cover girl, talking about her profession and all the movies she has lined up. Full story: NylonMag.com '19 Kids and Counting' Mom Michelle Duggar Opens Up About Eating Disorder Michelle Duggar battled bulimia as a teen. Full story: People.com Christian Heavy Metal Rock Star Pleads Guilty to Wife Murder Plot Tim Lambesis pleaded guilty on Tuesday, confessing that he plotted to have his estranged wife killed. Full story: UsWeekly.com Rihanna Bumps and Grinds Onstage with Rumored BF Drake Singers Rihanna and Drake showed their mutual affection while he was performing his "Would You Like a Tour" stop in Paris. See the video! Prev Post 'Girls' Star Allison Williams Engaged! Next Post Martin Scorsese, Robert De Niro to Be Honored with Unite4:Humanity Award Comments Related Posts Robin Thicke's Plan to Win Back Paula Patton Bad Behavior to Blame in Robin Thicke, Paula Patton Split Video! Robin Thicke Pours His Heart Out to Paula Patton Onstage Around the Web


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Leonardo DiCaprio Shirtless With Toni Garrn
Leo Has a Shirtless Water-Gun Fight, Because Why Not?
Leonardo DiCaprio's Summer of fun just keeps getting better. He's been on what seems like a perpetual vacation that's taken him from the South of France to Spain (with a stop in Italy!) over the past few weeks. In addition to tanning shirtless on the deck of a yacht with his girlfriend, Toni Garrn, he's hopped in the water and hung out with pals like Kevin Connolly. His latest adventure took place on land, though, where he and friends played a carefree game of Super Soaker tag, making for some pretty hilarious photos.
But the Summer getaway hasn't been all about relaxing — Leo and Toni had a big night out at the inaugural gala for Leo's foundation, which brought out a large group of stars. Jared Leto, Marion Cotillard, and Selena Gomez were just a few of the famous faces who showed up to support the cause.
Leo's had a few warm-weather vacations so far this season — he already hit the beach in Malibu, CA, for the Fourth of July and popped up shirtless in Miami, making it pretty clear he has the recipe for an awesome Summer perfected. Scroll through to see his latest snaps!
Kevin Connolly
Leonardo DiCaprio
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Celebrity Couples
- New details emerge in Patrick Dempsey's divorce — Us Weekly
- Sundance's most talked about performance will surprise you — HuffPost Celebrity
- Margot Robbie stole the show at Sundance Film Festival — Lainey Gossip
- Kim Kardashian tries a different look on the red carpet — Dlisted
- Find out who shunned the Mani Cam at the SAG Awards — ET
- 1 member of Blink 182 has called it quits — JustJared
- The SAG Awards red carpet gown that deserved more praise — Hollywood Tuna
- The official release date of Kelly Clarkson's new album — Pink Is the New Blog
- Karlie Kloss almost suffers a wardrobe malfunction — The Superficial
- Is Johnny Depp's new film Mortdecai any good? — The Blemish


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Jolly Green Giant (regultr) - BPL Lifetime Member
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Re: Re: Re: New JetBoil SolTi - $110 obo (regular $150) Final bump $110. |
Re: Re: New JetBoil SolTi - $120 obo (regular $150) Bump for price change. |
Re: New JetBoil SolTi - $125 obo (regular $150) Bump for price change. |
New JetBoil SolTi - $110 (regular $150) Okay, it's not new…..but it's close. I was one of those people who had the unfortunate experience of watching the metal fins on the bottom of my JetBoil Ti cup melt on its FOURTH use. I r... |
Re: Re: Re: Lukes Ultralite now using eVent Tall confirmed, at least in the PacLite. Great jacket and Luke is great to work with. Sure would be nice if other cottages made clothing for tall folks (I'm looking at you ZPacks). http://... |
Re: Tall person's support thread My favorites for tall stuff: Shelter - Lightheart Gear SoLong 6 Quilt/Bag - Western Mountaineering Clothing - Crapshoot. Try Luke's Ultralight for some custom-sized gear. Pack - Gossamer... |
Re: Why is this forum so inactive? Stagnation. http://www.backpackinglight.com/cgi-bin/backpackinglight/cottage_stagnation_recent_gems_2011.html |
Re: Cold sleeper gets FROZEN toes at night looking for UL but warm socks Are you against down booties for sleeping? They weigh very little, pack small and really make a tremendous difference. https://goosefeetgear.com |
Re: SALE: BOOK - Francis Tapon - Hike Your Own Hike Buy this book and the world will be a better place. No pressure, but the betterment of all of mankind is entirely up to you. |
Re: Re: SALE - BOOKS - Backpacking Light Magazine Sold to Levi. |
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Nunatak Teanaway Slipper It pains me to give a low rating to a company within the cottage industry of lightweight backpacking as I truly respect the customer service, innovation, and forward thinking that 99.9% of these compa... |


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Syllabification: lla·noPronunciation: /ˈlänō, ˈyä-
Definition of llano in English:
noun ( plural llanos)
(In South America) a treeless grassy plain.
Example sentences
- To find the snakes, he wades - barefoot - in the knee-deep water of the Venezuelan llanos, the lowland savannah that is flooded each year during rainy season.
- In general, Venezuela is usually divided into four major environmental regions: the coastal zone, the Andean mountain range, the llanos, and the Guiana Highlands.
- On a July morning in the llanos, or savannas, of Venezuela, the air is muggy and thick with mosquitoes.
Definition of llano in:
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Pronunciation: ˈditōˌhed
an unquestioning supporter of an idea or opinion…


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Summary: Tells the story of Parchman Farm, from its beginnings as a penal farm at the turn of the century to the 1972 court decision that sealed its fate. Memories and opinions of former convicts and employees form the heart of this narrative. This work is a greatly revised edition of the author's Brokered Justice: Race, Politics, and Mississippi Prisons, 1798-1992, which was published in 1993 by the Ohio State University Press. Taylor is professor of criminal justice at the University of Sou ...show morethern Mississippi. Annotation copyrighted by Book News, Inc., Portland, OR ...show less
Edition/Copyright: 99Filter by: All (13) | New (6) | Like New (1) | Very Good (1) | Good (5)
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Archive for the 'Recusal' Category
New Ethics Rules Affect Pennsylvania Judges Weighing Recusal
New ethics rules for Pennsylvania judges touch on numerous practices. One of the most potentially injurious practices involves judges taking campaign donations from parties who later appear before them in court.
One of the new rules advises judges to step aside from hearing a case if they learn that a party to it made a campaign donation to them "in an amount that would raise a reasonable concern" about fairness or impartiality, reports a Tribune Review article.
"We know that's a place where you can step on a land mine if you're not very careful," Pennsylvania Chief Justice Ronald Castille told the newspaper.
Because judicial campaign costs may vary widely in different parts of the state, vague rather than specific campaign contribution limits to trigger a judge's disqualification accommodate the range, Justice Castille said.
Earlier this year, a Center for American Progress analysis gave only eight of 39 states where judges are elected passing grades for adequately addressing the potential conflicts of interest that accompany high spending judicial elections. You can read about it by clicking here for Gavel Grab, and you can learn more about judicial recusal from Justice at Stake's web page about it.
No comments
Palestinian-American Defendant Seeks Recusal by U.S. Judge
Defense lawyers for Rasmea Odeh, a Palestinian-American woman charged with immigration fraud, have asked that U.S. District Court Judge Paul Borman of Detroit recuse himself from the case because of his extensive work to build support for Israel.
According to a Politico article, Odeh is accused of concealing the almost 10 years she served in an Israeli prison after her conviction in two bombings in Jerusalem, nearly 50 years ago. She says she was raped and tortured at the hands of Israeli officials and soldiers, and the U.S. legal system should not take notice of her conviction before an Israeli military court.
Her defense lawyers maintained about the judge, "Clearly, one who has been a life-long supporter and promoter of Israel and has deep ties to the State of Israel spanning over 50 years, who no doubts believes that Israeli is a great democracy and protector of human rights, cannot be 'reasonably' said to be impartial when these claims of torture and illegality are raised by a Palestinian defendant."
No commentsTwo Justices Decline to Recuse in High-Profile S.C. Case
The South Carolina Supreme Court heard arguments on Tuesday in a high-profile case involving the powerful House speaker. Two justices who had been asked by a watchdog group to recuse themselves did not do so, according to The Post and Courier.
In the oral arguments, a lawyer representing Attorney General John Wilson asked the court to set aside a state judge's decision directing Wilson to halt a criminal investigation of House Speaker Bobby Harrell.
Supreme Court Chief Justice Jean Toal, who won re-election as chief justice by the legislature this year, was actively supported by Harrell's electioneering. The executive director of Common Cause of South Carolina asked earlier that both Chief Justice Toal and her opponent, Associate Justice Constance Pleicones, consider recusing themselves (see Gavel Grab). Read more
No commentsArticle: Judges Part of 'An Ethical Mess in South Carolina'
The South Carolina Supreme Court will hear oral arguments next week in a closely watched case. Attorney General John Wilson is asking the court to set aside a state judge's decision directing Wilson to halt a criminal investigation of House Speaker Bobby Harrell.
There is a potential appearance of a conflict of interest in the proceeding, according to some observers, because Supreme Court Chief Justice Jean Toal, who won re-election as chief justice by the legislature this year, was actively supported by Harrell's electioneering. The executive director of Common Cause of South Carolina has asked that both Chief Justice Toal and her opponent, Associate Justice Constance Pleicones, consider recusing themselves.
The Center for Public Integrity published a lengthy article this week examining the entire matter, headlined "An ethical mess in South Carolina." It noted, "South Carolina is the only state other than Virginia with a system by which state lawmakers alone elect state court judges, including members of the Supreme Court." Read more
No commentsIn Oil Spill Litigation, Concerns About Justices and Sons
News media in Louisiana are reporting that two U.S. Supreme Court justices have sons who work at Gibson Dunn, the law firm representing BP in litigation over the Gulf of Mexico oil spill. One outlet, The Times-Picayune, says there are some calls for the justices to step aside from hearing any related proceedings in order to prevent an appearance of partiality.
Last week, a federal appeals court ordered that business claims against BP may proceed while BP awaits action by the high court on its appeal of Fifth U.S. Circuit Court of Appeals ruling. The appeals court had said that to win payment, it is not necessary for claimants to prove the oil spill harmed them directly. BP is asking reversal of the appeals court ruling.
Justices Samuel Alito and Antonin Scalia each has a son who works at Gibson Dunn, WWLT.com reported.
No commentsFederal Judge Says He Was 'Inexcusably Careless,' Apologizes
Chief Judge Randall R. Rader of the U.S. Court of Appeals for the Federal Circuit, who resigned last week his position at the court's helm (see Gavel Grab), has apologized for conduct that "crossed the ethics lines." He wrote an endorsement of the skills of a lawyer who appeared before him. Read more
No commentsAfter Recusal Disclosure, Chief Judge Steps Down
Chief Judge Randall R. Rader of the U.S. Court of Appeals for the Federal Circuit has resigned his position at the court's helm, while he will remain on its bench, the Wall Street Journal reported on Friday.
The judge's action came after the newspaper reported that Judge Rader recused himself from two patent cases after having endorsed an attorney participating in them.
Judge Rader's recusal came after the court had acted in the cases. Subsequently, the court "disclosed that he was now recused and reissued an opinion in one case and a judicial order in another," according to the Journal.
The resignation will take effect May 30.
No commentsOhio Editorial Urges Tougher Recusal Rules for Judges
More robust judicial recusal rules are needed in Ohio, given the weak rules that currently exist to police an important line between campaign spending and judges' conduct on the bench, a Toledo (Ohio) Blade editorial says.
"When lawyers, litigants, and other interest groups have financial stakes in how courts rule, it's unreasonable to think they would not expect a return on the investments they make in the form of campaign aid," the editorial explains. That's why strong recusal rule are important, it says. As for the weakness of the current ethics rules, it cites excerpts of a recent Center for American Progress report (see Gavel Grab).
While Ohio would be better served if it switched from electing judges to a system of merit selection, the editorial says, prospects for that reform are not good. It suggests recusal and disclosure reforms that could help, and it concludes:
No commentsMost States Get Failing Marks for Their Recusal Rules
Only eight of 39 states where judges are elected got passing grades from a Center for American Progress analysis for adequately addressing the potential conflicts of interest that accompany high spending judicial elections.
The CAP brief was entitled, "State Judicial Ethics Rules Fail to Address Flood of Campaign Cash from Lawyers and Litigants." It drew data about judicial election spending from "The New Politics of Judicial Elections 2011-12" by Justice at Stake and two partner organizations.
States that got passing grades were Arizona, 60; California, 75; Georgia, 70; Michigan, 70; Minnesota, 60; New York, 60; Utah, 65; and Washington, 65. The brief concluded:
No comments"The results of this study should alarm anyone who cares about impartial justice. Given the exponential increase in campaign cash in recent decades, justices in state supreme courts across America are hearing more and more cases that involve their campaign contributors. Polls show that this is causing the public to doubt judicial impartiality. Read more
Union donations and judicial recusal in Wisconsin
As the Wisconsin Supreme Court prepares to rule on a challenge to the state's "Act 10," which seeks to dismantle collective bargaining for most public workers, academics and interest groups are debating whether any of the justices should have to recuse themselves because they received campaign donations from public sector unions.
The Act, passed in 2011, was challenged by unions, who argue that its labor restrictions violated the state constitution. A Circuit Court judge agreed in 2012, and the case is now under consideration by the Supreme Court.
Wisconsin's rules requires judges to bow out of certain cases where their impartiality can be questioned. The Journal Sentinel reported on calls for recusal, along with the opinions of some legal scholars who stated that the donations in question were so small in relation to overall campaign spending that they may not require recusal.
No comments


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Originally written on
Fox Soccer
Last updated 11/20/14
Manchester United defender Patrice Evra has dismissed speculation linking him with a move back to France with Paris Saint-Germain .
Reports from the continent had suggested that big-spending PSG were ready to turn their attention to the 31-year-old left-back.
But France international Evra, who has been with United for over six years following his move from Monaco, insists he has no intentions of leaving before his contract has expired in 2014.
Talking to CanalPlus he said: "You are not serious to ask me such a question? I have one year remaining on my contract, I am playing at Manchester United and I am captain.
"You would need to kill [Sir Alex] Ferguson before anyone made me go to Paris! There is nothing in it and I am happy at Manchester."
However, the Senegal-born Evra admitted he was flattered to be linked with a move to Paris, where he was raised.
"It's always a pleasure to be wanted by a club such as Paris Saint-Germain, especially as I am Parisian. I have never hidden...
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I am a direct descendant of John Pea (also rendered Peay) b. 1794 NC. My family mythology says that this family line came to the United States from Ireland. John and Joseph are probably related . I have information on some of John's descendants. John was married to Elizabeth (surnamed Thompson by some researchers, but I'm not sure where they got the surname). They began their family in NC and moved to Kentucky. Notify Administrator about this message?
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Do you have any information on an Elzie Phillip Lovvorn, Marietta, GA. This was a friend's grandfather and she would like to know something about him. Please respond to firstname.lastname@example.com Thank you Notify Administrator about this message?
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Kannapolis police say two men broke into another man's residence twice in a period of three weeks. That apparently was one time too many.
Kristopher Garrett Welch, 20, and Andrew Christopher Calamari, 21, each of Kannapolis, were arrested Sunday morning after they allegedly broke into Christopher Lee Ludwik's home on Fisher Street, according to Kannapolis police. Investigators allege Welch and Calamari were the same men involved in a Feb. 7 home invasion at the residence.
This time, Ludwik was armed, according to authorities. Police say he pulled out a handgun when the two men allegedly broke into his home about 4:30 a.m. Sunday and began firing. One of the alleged attackers was shot in the leg, police say. During the scuffle, police say, one of the suspects grabbed the gun from Ludwik and struck him on the head. Kannapolis police did not say which of the suspects was injured.
The wounded man was arrested after arriving at CMC-Northeast for treatment, investigators say. Concord police found and arrested the other man.
Welch and Calamari each was charged with felony larceny and two counts of first-degree burglary. Welch also was charged with assault and battery, and Calamari was charged with assault with a deadly weapon and larceny of a firearm.
Police ask anyone with information about the two home invasions to contact them at 704-920-4000, at @kannapolis911 (Twitter) or at Kannapolis911 (Facebook). Information also can be left with Crime Stoppers at 704-932-7463.
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Theater News
Maria Aitken
Noël Coward's Private Lives to Close Out Shakespeare Theatre Company Season
Director Maria Aitken lends her Noël Coward expertise to the Washington, D.C. production.
Maria Aitken Replaces Nicholas Martin as Director of Huntington Theatre Company's The Seagull
The production of Anton Chekhov's classic stars Kate Burton.
Mark H. Dold, Gretchen Egolf Lead Betrayal at Huntington Theatre Company
Gretchen Egolf(courtesy of the company)
British director Maria Aitken will helm Huntington Theatre Company's production of Harold Pinter's Betrayal, running November 9-...


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Giuliano Vassalli
Giuliano Vassalli (25 April 1915 - 21 October 2009) was an Italian politician, lecturer and lawyer.
He was born in Perugia, son of Filippo Vassalli, a famous lecturer and lawyer. During World War II Vassalli was imprisoned by Nazi forces in Rome and subjected to torture. Pope Pius XII intervened personally on his behalf and was able to have his life spared [1]
Political career[edit]
After 1945 Vassalli joined the Italian Socialist Party; from 1968 to 1972 he was a deputy,and then from 1983 to 1987 a senator for this party. In 1987 Giovanni Goria named him Minister of Justice, he was confirmed along with Ciriaco De Mita and Giulio Andreotti. During this time he approved a new proceeding criminal code.
President Francesco Cossiga appointed him to the Constitutional Court of Italy 4th of February 1991 and woved nine days after. He was Vice-president of the court from 1996 to 1999 under Renato Granata, and its President from 11 November 1999 to 13 February 2000.
Although in his later years Vassalli retired from public life, he was sometimes asked for his advice. During 2001 Prime Minister Silvio Berlusconi sought his advice over the European Arrest Warrant and Vassalli answered that it was a violation of the principles of natural justice.
- ^ Peter Hebblethwaite, Paul VI, Paulist Press, New York, 1978, p.701


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The Whitehall Hotel
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Nestled at the base of the West Pennine Moors, centrally located between BOLTON and BLACKBURN with DARWEN we provide the perfect retreat for either BUSINESS or LEISURE. Our resident lounges are perfect to either relax with a daily newspaper by an open fire or make use of our COMPLIMENTARY wifi which will extend into the courtyard - weather permitting!
We have two dining options either MJs bar which offers a bar/lounge menu or a more formal option in the dining room.
Our spacious BEDROOMS on the groundfloor have recently been refurbished. We also have several feature bedrooms with bespoke furniture, 4 poster beds and 6 jet spa baths. Complimentary hospitality trays are available with speciality teas and coffee with locally produced cookies. Secure parking is available to the front of the hotel with preventative cctv coverage.
We are not a large hotel and your only interruption to a peaceful nights sleep may be the occasional bird call.
No stay with us would be complete without our freshly prepared breakfast. Enjoy a healthy option of fruits, cereals or if you prefer a hearty English BREAKFAST. Cooked to order as you prefer choose eggs, bacon, sausages, black pudding, beans - in fact why not have the lot!
Be assured, irrespective of the time whatever you decide to have will be accompanied with friendly service.


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Alessi Magic Bunny Magnet
This new family of objects is part of an ongoing collaboration with the National Palace Museum in Taiwan, which began in 2007 with the ''Chin Family''. Coming into close contact with the extraordinary collection of art works at the National Palace Museum set Stefano's imagination racing, inspiring him to explore the millennial tradition of Chinese applied arts and develop his own contemporary interpretation of them. Many of pieces from the Orientales series are very reminiscent of Giovannoni's works in the ''Chin Family''. Characters of flowers and fruits, each performing the roles of domestic household objects, are made partly in hand-decorated plastic and fine bone china. The magic of Giovannoni's design lies in the fact that these objects recall the grace of 18th century ceramic trinkets, while at the same time being utterly contemporary. Giovannoni's whimsical style and penchant for vibrant colors are very apparent in the hand-decorated ''Lily Bird'' Soy Sauce Container. Made of fine china, the ''Lily Bird'' features a double groove to prevent it from falling during use. You'll know when the lid is closed when the red shades on the head and body coincide.
About the Designer
Stefano Giovannoni Italy (1954 - )
Born in La Spezia in 1954, Stefano as an architect and designer was taught by Buti at Florence University. In the early 80Æs he founded the King-Kong Production Group along with Guido Venturini. His explosive talent enables him to embody a complex system of 'affective codes' in his projects. This great approach won him the title of 'Super and Popular' designer in the 90Æs. A technical designer par excellence, he has industrial design in his DNA and the capacity to understand public sentiment like no other designer. His projects began with the "Girotondo" (1989 designed with Guido Venturini) and the first transparent objects in plastic from the "Family Follows Fiction" series (1993). There is a huge number of his items in the "Officina Alessi", "Alessi" and "A di Alessi" catalogues.
Alessi Magic Bunny Magnet
Weights & Dimensions
Material: Plastic
| Country of Manufacture: Italy |
About the Manufacturer
Alessi's intention, from the beginning, was to be highly innovative and ready to experiment with cookware. With this in mind, the Alessi family venture explored new materials and novel design in becoming one of the finest and most well-known contemporary cookware brands. Alessi prides itself on its unique collaboration with designers and architects to produce beautiful, quality cookware. Among some of the best known of the Alessi's products are Richard Sapper's kettle with a two-tone whistle, Michael Graves' kettle with bird shaped whistle, and Philippe Starck's Juicy Salif citrus squeezer.Alessi Magic Bunny Magnet
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5 star | 3 | |
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Cute Rita from Austin, TX Magnet on it is not super strong but the item has its charm. |
disappointed on how small this thing is J from Whitestone, NY– Verified Buyer I was totally disappointed when I received this in mail, soooooo small, and expected there were 10 of these magnets in this order, as described in the description. I didn't realize they were selling one only.... $10 for a magnet is like a Disney's dream! Oh well, allmodern.com staff was helpful and offered me compensation for next purchase, as it wasn't worth it to return it. |
just so very cute Michelle from Omaha, NE– Verified Buyer this was very very cute just loved it very very much |
Cute hamdan from granada hills, CA– Verified Buyer Wife ordered it and its very cute. The magnet sticks to the board securely. |
Magic Bunny magnet Susan from Haverhill, MA– Verified Buyer Blue Magic Bunny magnet mirrors the pink Magic Bunny toothpick holder on my kitchen hutch. |
Magic Bunny Magnet is also sometimes listed under the following manufacturer numbers: ASG16 AZM, ASG16 GRM


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Dr. Edward M. Zoratti, MD
Allergy & Immunology, Henry Ford Medical Center
- 3500 15 Mile Rd
Sterling Heights, MI 48310 - 1 Office Location
- (586) 977-9300
Dr. Edward M. Zoratti graduated from the Wayne State University School of Medicine in 1985. He works in Sterling Heights, MI and specializes in Allergy & Immunology. Dr. Zoratti is affiliated with Henry Ford Medical Center, Henry Ford Wyandotte Hospital and Henry Ford Hospital.
Gender: Male
Years in Practice: 30 Years
Languages Spoken: English
Medical School: Wayne State University School of Medicine
Graduation Date: 1985
Hospital Affiliations: Henry Ford Medical Center, Henry Ford Wyandotte Hospital, Henry Ford Hospital
Specialties: Allergy & Immunology


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A Bedtime Mix from Sam Smith helps Annie round off your weekend. #musicalhotwaterbottle.
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Shameless Review: "Aunt Ginger"
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Forum - View topicANNtv - 2008-02-02
Goto page 1, 2 Next Note: this is the discussion thread for this article |
View previous topic :: View next topic | |||
Author | Message | ||
samuelp Posts: 1585 Location: Tokyo, Japan |
Hey, I was thinking...
You should really take these shows (or at least the interviews, etc), and subtitle them into Japanese, and let your sister site animeanime put up the videos for Japanese fans. I think a lot of the news and views discussed on the shows would be very interesting back in Japan (especially the interviews), and it would help promote cross-ocean understanding. Animeanime has had a lot of news items about R1 anime lately, so I think your weekly(ish) news show would be neat for them to have. (Although I'm not sure they have a streaming video capability like you do). |
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Lolotakun Posts: 45 Location: Luxembourg |
Nice, but the stuttering during the really long interview was difficult to suffer. If Chris Tribbey had gotten out of his wholesale warehouse, some audio editing could have been done to cut out the repetitions and the "er..."s and "you know"s. Maybe some of Mario Buenos questions should have been reshot, they contrast highly with his otherwise fluent delivery.
But content-wise very good, thank you! |
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Gage Posts: 479 Location: United States |
The footage seemed a bit blurry to me than normal. Footage being the whole show in general although it wasn't bad to the point of me not knowing what was going on. Although you did note technical difficulties with the audio so maybe there were issues with quality. Was a good show all in all. | |||
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tueac Posts: 33 |
The way of releasing the whole season or 13 episodes at a time is already done here in Singapore... and it really ok. | |||
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Yoshball Posts: 99 Location: Portland, OR |
I literally laughed out loud when Mario went into his "relaxed" position for the interview. All of a sudden there was a random knee in camera and it took me a second to figure out how it got there. If he's going to sit, then at least show the chair or start at some other angle. Otherwise its one camera shot of him standing then camera cut to a floating knee. Weird. | |||
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tasogarenootome Posts: 558 |
Really enjoying ANNtv, nice work! I liked the interview and am looking forward to the one next week with Oreskaband! | |||
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Murasakisuishou Posts: 1469 Location: NE Ohio |
That was an interesting interview. I have to say, I really like the whole idea of complete seasons instead of singles; you still have something nice to put on your shelf (what I don't like about digital downloads - I like to have a hard copy), but it's less expensive. Usually when you buy a single you're down for the entire series anyway. If they throw in some nice boxes and LE extras or something (a la Haruhi), there's a nice incentive for people to buy them.
As far as ADV goes, they can crap out as long as Appleseed: Ex Machina gets released first. :p ....*knocks on wood* |
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v1cious Posts: 5058 Location: Fresno, TX |
very eye-opening interview. seemed really awkward for some reason though. | |||
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Dargonxtc Posts: 4463 Location: Nc5xd7+ スターダストの海洋 |
Yay, I finally got the stream to work! I was having trouble since it was posted last night and this morning for some reason.
Interesting interview, and I think it should be clear to everyone to prepare for major shrinkage. It did remind me of Animeigo, aren't they supposed to be releasing something new pretty soon though? |
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Gage Posts: 479 Location: United States |
When exactly will this video be uploaded to your DailyMotion page? I'm anxious to get it up on my blog soon. | |||
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vtnwesley Posts: 171 Location: Natrona Heights, PA |
I think the problem with boxes is that japanese companies likely tell them not to do that. I'd think in the mind of a marketting guy, boxsets are for old classic content which has made its money or budget shows which won't sell otherwise. It's probably going to take some open mindedness on all parts to get real boxsets instead of singles. I feel your pain though. I'm for boxsets all the way. Digital distribution is a trickier beast though. 1. It needs to be viewable on more than just the PC. Not all services are good about this. iTunes had the ipods and appletv unit which is kind of nice 2. When you stuff gets messed up, you need some kind of method for re-download, even if it's DRMd or watermarked. Forced CD-R or DVD-R data backups wouldn't be an outright bad idea either. 3. There needs to be some future support assurance system in place. I buy my junk through distributor "A" and they go out of business. Is their proprietary DRM going to lock me to non-functional software? When I reinstall Windows or upgrade, will legalanimedl.fake.com still even let me install their client software? All big considerations. I think iTunes has come a long way and is getting interesting, but digital distribution has a long way to go. BTW, Much better ANNTV this week. No complaints. Hope you guys interview some American voice actors at some point too. You should start with Vic "FMA" "hard last name to remember". Many people like him including me, and he seems accessible for that kind of fandom type stuff. |
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championferret Posts: 590 |
When I read Ellie was 'off this week', I was thinking Mario doing all the talking might look weird/awkward.
But I think he carried it really well. Good job! |
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jsevakis ANN Director of New Media Posts: 1545 Location: Los Angeles, CA |
It's up now. |
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Randall Miyashiro Posts: 2451 Location: A block away from Golden Gate Park |
Definitely my favorite episode so far. The industry insider interview was nicely done. I'm not a big fan of ANNtv restating what is already posted in the news section in text. Interviews like this one are much better. What I really would love to see is an interview with someone from either ADV or Genoen although don't expect it to happen. | |||
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cloud1989 Posts: 274 |
I like the idea of complete collections, mainly because I've became to afraid to buy singles. | |||
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Man arrested for tearing up ballot
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