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case in point
Definition of case in point in English:
An instance or example that illustrates what is being discussed: the "green revolution" in agriculture is a good case in point
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- What is now happening on the Shankill Road is a classic example of the case in point.
- The Royal Museum that houses the first cloned sheep named Dolly, the National Gallery of Scotland along with quite a few national museums are paradigmatic cases in point.
- Alas, this principle is applied more generally as governments assume control of the delivery or standard of services of one sort or another: currently the National Health Service and the railways are cases in point.
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Pronunciation: ˌapəˈTHedik
showing or feeling no interest, enthusiasm, or concern


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Snapchat's Discover lets media publishers enter their ephemereal world
Messaging apps are continually evolving into more than just ways to exchange messages or even pictures. With so many out there like LINE, Viber, WhatsApp, etc, competitors are pulling out all the stops to become the app of choice. SnapChat has a following all its own, with the ephemeral nature of the pictures exchanged. Now they're branching out into publishing as they introduce a new feature called Discover, where media companies can now also enter their realm with pictures, videos, and stories.

subset: commoncrawl


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Accepted Insurance
Accepted Insurance ?
Acceptance of particular insurance plans may vary, based on different office locations. As a result, we've listed plans accepted at different locations. Click on any insurance company to see which specific plans are accepted by Dr. Howell-Canada.
3223 E Palmr Wasl Hwy, Ste 1, Wasilla, AK 99654 | |||
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Sep 1, 2011 10:39 AM
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There comes a point where putting the same number of candles on your birthday cake as the number of years you're turning becomes a significant fire hazard. When you reach that age, there's no reason to stop celebrating. But, in the interest of safety, let... [More]
Aug 30, 2011 8:15 AM
Far beyond the Chia Pet that looked like our high school English teacher, the Chia Herb Garden ($16) is just in time for 'more herbs, less salt day.' We think a nice little herb garden in your window is a good way to honor it,... [More]
Aug 29, 2011 10:30 AM
We've got the perfect favor for your Vermont (actually, anywhere) fall wedding! Use one of these charming Maple Syrup Favors ($5) as a place setting, send them as thank you gifts, or even forget the wedding and dress up your Thanksgiving table.... [More]
If you've got a product or service that you'd like to promote on Outblush, click here to find out about our advertising opportunities. |
Aug 25, 2011 9:46 AM
It's never too early to buy your boyfriend a costume for Halloween, especially when you feel it really fits his personality. But Mommy, I Don't Want To Grow Up Adult Costume ($56)... [More]
Aug 23, 2011 8:45 AM
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Aug 22, 2011 10:49 AM
Get out your sexy SpongeBob costumes, it's Halloween! Okay, so actually it's just late August but before you know it, the back to school section at Target will disappear only to be filled with aisles and aisles of October goodness!!! We have big party plans... [More]
Aug 18, 2011 10:35 AM
Too early to be thinking about Halloween costumes? Oh no. We're thinking that with this kepi ($15) and a good rampage through the applique rack at the nearest craft store, we'd be well on our way to dressing up as Louis Renault from Casablanca -... [More]
Aug 18, 2011 8:15 AM
So what if it looks like it's for a kid's room or party! We totally want one of these to celebrate ourselves on our birthday! Made of whimsical birds hand-crafted from felt, each bird is grasping a letter to spell out your name. Maybe they'll... [More]
Aug 16, 2011 10:46 AM
The only thing better than planning our own Halloween costumes is planning a kid's Halloween costume! And when they're young, they don't really care what they are...mwhahahha...you can dress 'em up in anything instead of tactfully trying to change their minds about being something with... [More]
Aug 15, 2011 9:15 AM
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Aug 9, 2011 9:59 AM
Sending out a cheesy mapquest map for your event pretty much ensures everyone will use their idiotic smart phone to grab destination directions. You know what that means- strange detours, dead batteries, and a party dominated by driving horror stories. You didn't go through all... [More]
Aug 1, 2011 8:44 AM
Halloween may still be a few month away, but we're such fans, we can't help browse for spooky things! (Why yes, we've also had our costumes planned for months.) We totally love The 12' Inflatable Headless Horseman ($300) at Hammacher Schlemmer because it's a TWELVE... [More]
Jul 4, 2011 10:40 AM
Waste not, want not, people. The Smart Planet Eco Party Plate ($20 / set of four) are great for summer parties and won't look out of place at all. Sure, we'll have more dishes, but we'll have less paper in the trash, so it's worth... [More]
Jun 28, 2011 12:09 PM
America! America! God shed his grace on thee And crown thy good with brotherhood From sea to shining sea! This year, we celebrate all of America. Not just with great clothes, fantastically patriotic jewelry or color approriate footwear. But also by truly honoring the melting... [More]
Jun 24, 2011 9:57 AM
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Jun 21, 2011 8:07 AM
The Bordello Teeze-06R Pump in Iridescent Rhinestones ($158). Because it is never, ever, ever too early to start planning your Slutty Dorothy costume for Halloween. Not planning to be Slutty Dorothy? How about Naughty Nemo? It's just as classy.... [More]
Jun 16, 2011 11:55 AM
We think these custom Paloma's Nest Wooden Party Crowns ($28 each) are meant to add a whimsical touch for a child's birthday party. But um, we're totally coveting them. Cheap plastic birthday tiaras pale in comparison to these artfully made crowns. Customize them with names,... [More]
Jun 9, 2011 8:35 AM
Is your dad a true nerd at heart? Total geek? Way too smart for his own good? If so, we've found the perfect Father's Day card for Chief Smarty Pants! My Dad's a Big Ol' Geek Card ($5)... [More]
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POV's Executive Director Simon Kilmurry shares some exciting news about POV alum Ido Haar. For those of you close to Westchester County, New York, POV alum Ido Haar, the filmmaker of 9 Star Hotel (POV 2008), will be featured as the third International Filmmaker-in-Residence at Jacob Burns Film Center's Media Arts Lab in Pleasantville. Ido
Continue reading this entry »ido haar
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Join us for a special congressional screening of The Genius of Marian at the Capitol Hill Visitor Center. Speakers will include David Hyde Pierce, actor and activist, Senator Debbie Stabenow (D-Mich), Simon Kilmurry, executive producer of POV, Ed White, husband of Pamela White, and Devon White Angelini, daughter of Pamela White. The event is free and open to the public, but seating is limited and RSVP is required: http://bit.ly/1zu583q.
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recipe reviews for:
"Delicious pork loin roasted with bacon that the whole family will love. Serve with potatoes or any of your favorite veggies."
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US 7119254 B2 Abstract The present invention relates to a root knot nematode responsive promoter isolated from Arabidopsis thaliana elongation specific endo-1, 4-beta-glucanase (cel1) gene, and a method of producing transgenic nematode and insect resistant plants and cells using cel1 promoter.
Claims(22) 1. A DNA construct which comprises, in the 5′ to 3′ direction:
(a) a functional root knot nematode responsive promoter isolated from an Arabidopsis thaliana elongation specific endo-1,4-beta-glucanase gene; and
(b) a heterologous DNA positioned downstream from said root knot nematode promoter and operatively linked therewith, said heterologous DNA encoding a product disruptive of nematode attack or an insecticidal protein.
2. The DNA construct according to
3. The DNA construct according to
4. The DNA construct according to
5. The DNA construct according to
6. A plant cell transformed with the DNA construct according to
7. A method of producing a transformed plant, comprising regenerating a plant from the plant cell according to
8. An Agrobacterium tumefaciens cell containing the DNA construct according to
9. A method of producing a transformed plant, comprising infecting a plant cell with the Agrobacterium tumefaciens cell according to
10. A microparticle carrying the DNA construct according to
11. A method of making a transformed plant, comprising propelling the microparticle according to
12. A plant cell protoplast comprising the DNA construct according to
13. A method of producing a transformed plant, comprising regenerating a plant from the plant cell protoplast according to
14. A transformed plant comprising the DNA construct according to
15. The transformed plant according to
16. The transformed plant according to
17. A transformed plant according to
18. A plant seed from the plant according to
19. A method of producing a root knot nematode resistant plant, comprising transforming the plant with a DNA construct comprising:
a) a functional root knot nematode responsive promoter isolated from an Arabidopsis thaliana elongation specific endo-1, 4-beta-glucanase gene; and
(b) a heterologous DNA positioned downstream from said root knot nematode promoter and operatively linked therewith, said heterologous DNA encoding a product disruptive of nematode attack.
20. The method according to
21. The method according to
22. A root knot nematode resistant plant produced by the method of
Description This application claims the benefit of U.S. provisional application Ser. No. 60/178,883, filed Jan. 28, 2000, the disclosure of which is incorporated by reference herein in its entirety. This invention relates to tissue-specific gene promoters, and particularly relates to a promoter which is responsive to the root knot nematode. A promoter is a DNA sequence which flanks a transcribed gene, and to which RNA polymerase must bind if it is to transcribe the flanking gene into messenger RNA. A promoter may consist of a number of different regulatory elements which affect a structural gene operationally associated with the promoter in different ways. For example, a regulatory gene may enhance or repress expression of an associated structural gene, subject that gene to developmental regulation, or contribute to the tissue-specific regulation of that gene. Modifications to promoters can make possible optional patterns of gene expression, using recombinant DNA procedures. See, e.g., Old and Primrose, Principles of Gene Manipulation (4th Ed., 1989). U.S. Pat. No. 5,459,252 to Conkling and Yamamoto describes a root specific promoter designated RB7, which was identified in tobacco. U.S. Pat. No. 5,837,876 to Conkling et al. describes a root cortex specific gene promoter designated the RD2 promoter, which was also identified in tobacco. Rather than use a promoter that is constitutively active, it is desireable to have promoters that are responsive to particular stimuli. In particular, if a promoter is responsive to a particular pathogen, then that promoter could be used to impart selective disease resistance to that pathogen through expression of a transgene that disrupts that pathogen. U.S. Pat. No. 5,750,386 to Conkling, Opperman and Taylor describes pathogen resistant transgenic plants in which a nematode-responsive element is operatively associated with a nucleotide of interest (in this case, a gene encoding a product toxic to plant cells). One nematode responsive element was a deletion fragment of the RB7 root specific promoter described above. U.S. Pat. No. 5,589,622 to Gurr et al. suggests nematode resistant transgenic plants in which cells of the plant contain a heterologous construct comprising a nematode responsive promoter operatively associated with a product disruptive of nematode attack. However, the DNAs disclosed by Gurr et al. as nematode responsive promoters do not appear to represent such promoters, and instead appear to represent extraneous or irrelevant DNA. To impart useful traits to plants by the expression of foreign genes using genetic engineering techniques, a variety of pathogen-responsive promoters will be required to allow traits to be expressed selectively, in the appropriate plant tissues, and at the appropriate times. Accordingly, there is a continued need for pathogen responsive elements that operate in plant cells. The present invention is based on the discovery that the elongation specific endo-1,4-β-glucanase (cel1) promoter of Arabidopsis thaliana, described in U.S. Pat. No. 6,005,092 to O. Shosoyev and Z. Shani (Dec. 21, 1999), and Shani et al., Plant Molecular Biology 34, 837�842 (1997), is upregulated in root-knot nematode feeding cells (i.e., giant cells). Plant parasitic nematodes cause approximately 100 billion dollars annually in crop loses worldwide. The root knot nematode has a host range of over 2000 plant species, and is one of the most damaging nematodes. Accordingly, a first aspect of the present invention is an isolated DNA molecule which directs root knot nematode responsive transcription of a downstream heterologous DNA segment in a plant cell (i.e., a promoter), and the use thereof in providing or imparting nematode resistance to plants and plant cells. A further aspect of the present invention is construct comprising a promoter as described above and a heterologous DNA segment (i.e., a DNA segment not naturally associated with that promoter) positioned downstream from, and operatively associated with, the promoter. The heterologous DNA segment preferably encodes a product disruptive of nematode attack (i.e., a product that hinders or interferes with the ability of a nematode to feed upon a plant cell, or establish a feeding site in relationship to a plant cell, when that product is expressed in a plant cell). Further aspects of the present invention are plant cells containing the above described constructs, methods of making transformed plants from such plant cells, the transformed plants comprising such transformed plant cells, and the use of the foregoing to impart resistance to root knot nematodes to plants. Various preferred embodiments of the present invention are set forth below. These embodiments are not intended to provide a detailed catalog of all manner in which the instant invention may be carried out, as numerous variations will be apparent to persons skilled in the arts to which the invention pertains. Accordingly, the following is set forth for illustrative purposes, and is not intended to be limiting of the invention. 1. Root Knot Nematodes. The invention may be carried out to protect plants from root knot nematodes (Meloidogyne spp.). Root-knot nematodes are sedentary endoparasites with an extremely intimate and complex relationship to the host plant. The infective second stage juvenile (J2) is free in the soil. Upon location of a host root, the J2 penetrates the root intercellularly in the region just posterior to the root cap and migrates to the developing vascular cylinder. The nematode then orients itself parallel to the cylinder and injects glandular secretions into the plant cells surrounding its head, resulting in the initiation of nematode feeding cells. These 5�7 cells undergo rapid nuclear divisions, increase tremendously in size, and become filled with pores and cell wall invaginations. The feeding site cells, or �giant cells�, function as super transfer cells to provide nourishment to the developing nematode. During this time, the nematode loses the ability to move and swells from the normal eel shaped J2 to a large, pear shaped adult female. As the nematode feeds on the giant cells, parthenogenic reproduction results in the disposition of 300�400 eggs. This entire process occurs over the span of 20�30 days, and root-knot nematodes may complete as many as 7 generations during a cropping season. Thus, in addition to delivering at the feeding site a product that is toxic to the nematode, it will be seen that, by causing the plant itself to kill or disable the cells upon which the pathogen must feed, the pathogen will be much less successful at infecting the plant. 2. Promoters. As used herein, a nematode responsive (or �nematode inducible�) promoter refers to a promoter that (a) does not normally drive transcription in a plant cell except when that cell resides in tissue infected by a root knot nematode, or (b) normally drives transcription in a plant cell, and which drives increased levels of transcription when that cell resides in tissue infected by a root knot nematode. The promoter may be a naturally occurring promoter, may comprise a nematode responsive element isolated from a naturally occurring promoter, or may be a synthetic promoter. A preferred promoter for use in the present invention is the elongation specific endo-1,4-β-glucanase (cel1) promoter of Arabidopsis thaliana, has been described by Z. Shani, M. Dekel, G. Tsabary and O. Shoseyov, Plant Molecular Biology 34, 837�842 (1997), and has been assigned EMBL, GenBank and DDBJ Nucleotide Sequence Database accession number X98543. Id. at 837, 839. This promoter is referred to herein as an Arabidopsis cel1 promoter, and is set forth herein as SEQ ID NO: 1. The Arabidopsis cel1 promoter and other promoters that may be used to carry out the present invention is also disclosed in U.S. Pat. No. 6,005,092 to O. Shoseyov and Z. Shani, issued Dec. 21, 1999, the disclosure of which is incorporated by reference herein in its entirety. Other DNAs that hybridize to an Arabidopsis cel1 promoter under high stringency hybridization conditions as described below, and which encode a nematode responsive promoter (particularly a root knot nematode responsive promoter) may also be used to carry out the present invention. High stringency hybridization conditions which will permit homologous DNA sequences (e.g., other natural plant DNA sequences) to hybridize to a DNA sequence encoding an Arabidopsis cel1 promoter are well known in the art. For example, hybridization of such sequences to a DNA encoding an Arabidopsis cel1 promoter may be carried out in 25% formamide, 5�SSC, 5�Denhardt's solution, with 100 μg/ml of single stranded DNA and 5% dextran sulfate at 42� C., with wash conditions of 25% formamide, 5�SSC, 0.1% SDS at 42� C. for 15 minutes, to allow hybridization of sequences of about 60% homology. More stringent conditions are represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, 0.1% SDS at 60� or even 70� C. using a standard in situ hybridization assay. (See Sambrook et al., Molecular Cloning, A Laboratory Manual (2d Ed. 1989)(Cold Spring Harbor Laboratory)). In general, plant DNA sequences which code for nematode responsive promoters and which hybridize to the DNA sequence encoding the nematode responsive elements disclosed herein will be at least 75%, 80%, 85%, 90% or even 95% homologous or more with the sequences of the DNA encoding the nematode responsive elements disclosed herein. It will be apparent that other sequence fragments from the promoter 5′ flanking region, longer or shorter sequences, or sequences with minor additions, deletions, or substitutions made thereto, can be prepared which will also encode a nematode responsive promoter, all of which are included within the present invention. 3. Heterologous DNAs and Expression Cassettes. DNA constructs, or �expression cassettes,� of the present invention include, 5′-3′ in the direction of transcription, a nematode responsive promoter of the present invention, a heterologous DNA segment operatively associated with the promoter, and, optionally, transcriptional and translational termination regions such as a termination signal and a polyadenylation region. All of these regulatory regions should be capable of operating in the transformed cells. The 3′ termination region may be derived from the same gene as the transcriptional initiation region or from a different gene. The term �operatively associated,� as used herein, refers to DNA sequences contained within a single DNA molecule which are associated so that the function of one is affected by the other. Thus, a promoter is operatively associated with a gene when it is capable of affecting the expression of that gene (i.e., the gene is under the transcriptional control of the promoter). The promoter is said to be �upstream� from the gene, which is in turn said to be �downstream� from the promoter. Heterologous DNAs used to carry out the present invention may encode any product that is disruptive of nematode attack when that DNA is transcribed (and, where applicable, translated) in a plant cell, including but not limited to proteins, peptides, and non-protein products such as antisense RNAs, ribozymes, other nucleic acids that suppress expression by sense strand suppression or triplex formation, etc. (see, e.g., U.S. Pat. No. 4,801,540 (Calgene, Inc.)). The heterologous DNA may encode a product that is toxic to the plant cells, as described in U.S. Pat. No. 5,750,386 to Conkling et al. A wide variety of protein or peptide products which are toxic to plant cells can be used, including (but not limited to) enzymes capable of degrading nucleic acids (DNA, RNA) such as nucleases, restriction endonucleases micrococcal nucleas, Rnase A, and barnase; enzymes which attack proteins such as trypsin, pronase A, carboxypeptidase, endoproteinase Asp-N, endoproteinase Glu-C, and endoproteinase Lys-C; ribonucleases such as RNase CL-3 and RNase T1, toxins from plant pathogenic bacteria such as phaseolotoxin, tabtoxin, and syringotoxin; lipases such as produced from porcine pancrease and Candida cyclindracea, membrane channel proteins such as glp F and connexins (gap junction proteins, and antibodies which bind proteins in the cell so that the cell is thereby killed or debilitated. Genes which produce antibodies to plant cell proteins can be produced as described in W. Huse et al., Science 246, 1275�1281 (1989). Proteins to which such antibodies can be directed include, but are not limited to, RNA polymerase, respiratory enzymes, cytochrome oxidase, Krebs cycle enzymes, protein kinases, aminocyclopropane-1-carboxylic acid synthase, and enzymes involved in the shikimic acid pathway such as enolpyruvyl shikimic acid-5-phosphate synthase. One preferred heterologous DNA is a structural gene encoding mature Bacillus amyloliquefaciens RNase (or Barnase). See, e.g., C. Mariani et al., Nature 347, 737�741 (1990); C. Paddon and R. Hartley, Gene 40, 231�39 (1985). Note that the toxic product may either kill the plant cell in which it is expressed or simply disable the cell so that it is less capable of supporting the pathogen. It is preferred, particularly where the plant is a food plant, that the plant-toxic product be non-toxic to animals, and particularly be non-toxic to humans. The heterologous DNA may encode any other product disruptive of nematode attack, including but not limited to those described in U.S. Pat. No. 5,589,622 to Gurr et al. (e.g., products toxic to the nematode). Thus the heterologous DNA may encode a Bacillus thuringiensis crystal protein toxic to insects. Strains of B. thuringiensis which produce polypeptide toxins active against nematodes are disclosed in U.S. Pat. Nos. 4,948,734 and 5,093,120 (Edwards et al.). Again note that the toxic product may either kill the nematode attempting to feed on the plant cell in which it is expressed or simply disable the nematode so that it is less capable of feeding on the plant cell or establishing a feeding site. For example, the heterologous DNA may encode a peptide, antibody or the like that disrupts feeding by interacting with the ingestion or digestion of food such as one of the antibodies described for soybean cyst nematode including that against the dorsal pharyngeal gland (Atkinson et al, 1988 Annals of Applied Biology 112, 459�469), modified as necessary for specificity to the root knot nematode, using the procedures for transgenic expression of antibodies in plants described by Hiatt, A. Gafferkey, R. C. & Bowdish, K. (1989 Production of Antibodies in Transgenic Plants Nature 342, 76�78). Again it is preferred, particularly where the plant is a food plant, that the nematode-toxic product be non-toxic to other animals, and particularly be non-toxic to birds, reptiles, amphibians, mammals and humans. Where the expression product of the gene is to be located in a cellular compartment other than the cytoplasm, the structural gene may be constructed to include regions which code for particular amino acid sequences which result in translocation of the product to a particular site, such as the cell plasma membrane, or secretion into the periplasmic space or into the external environment of the cell. Various secretory leaders, membrane integration sequences, and translocation sequences for directing the peptide expression product to a particular site are described in the literature. See, for example, Cashmore et al., Biotechnology (1985) 3:803�808, Wickner and Lodish, Science (1985) 230:400�407. The expression cassette may be provided in a DNA construct which also has at least one replication system. For convenience, it is common to have a replication system functional in Escherichia coli, such as ColE1, pSC101, pACYC184, or the like. In this manner, at each stage after each manipulation, the resulting construct may be cloned, sequenced, and the correctness of the manipulation determined. In addition, or in place of the E. coli replication system, a broad host range replication system may be employed, such as the replication systems of the P-1 incompatibility plasmids, e.g., pRK290. In addition to the replication system, there may be at least one marker present, which may be useful in one or more hosts, or different markers for individual hosts. That is, one marker may be employed for selection in a prokaryotic host while another marker may be employed for selection in a eukaryotic host, particularly the plant host. The markers may provide protection against a biocide, such as antibiotics, toxins, heavy metals, or the like; may provide complementation by imparting prototrophy to an auxotrophic host; or may provide a visible phenotype through the production of a novel compound in the plant. Exemplary genes which may be employed include neomycin phosphotransferase (NPTII), hygromycin phosphotransferase (HPT), chloramphenicol acetyltransferase (CAT), nitrilase, and the gentamicin resistance gene. For plant host selection, non-limiting examples of suitable markers are beta-glucuronidase (GUS) (providing indigo production), luciferase (providing visible light production), NPTII (providing kanamycin resistance or G418 resistance), HPT (providing hygromycin resistance), and the mutated aroA gene (providing glyphosate resistance). An advantage of the present invention is that two or more promoters can be �daisychained� to a single structural gene. Where each promoter is responsive to a different pathogen, the plant is then provided with resistance to a plurality of promoters. For example, a second promoter may be positioned upstream from the structural gene and operatively associated therewith so that the structural gene is associated with a plurality of promoters, with each of the promoters activated by a different plant pathogen. Still more promoters can be included if desired. Other promoters that may be used in conjunction with the instant promoter are described in U.S. Pat. No. 5,750,386 to Conkling et al. The various fragments comprising the various constructs, expression cassettes, markers, and the like may be introduced consecutively by restriction enzyme cleavage of an appropriate replication system and insertion of the particular construct or fragment into the available site. After ligation and cloning, the DNA construct may be isolated for further manipulation. All of these techniques are amply exemplified in the literature. See, e.g., Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1982). 4. Plant Transformation Vectors and Techniques. A vector is a replicable DNA construct. Vectors which may be used to transform plant tissue with DNA constructs of the present invention include both Agrobacterium vectors and ballistic vectors, as well as vectors suitable for DNA-mediated transformation. Agrobacterium tumefaciens cells containing a DNA construct of the present invention, wherein the DNA construct comprises a Ti plasmid, are useful in methods of making transformed plants. Plant cells are infected with an Agrobacterium tumefaciens to produce a transformed plant cell, and then a plant is regenerated from the transformed plant cell. Numerous Agrobacterium vector systems useful in carrying out the present invention are known. For example, U.S. Pat. No. 4,459,355 discloses a method for transforming susceptible plants, including dicots, with an Agrobacterium strain containing the Ti plasmid. The transformation of woody plants with an Agrobacterium vector is disclosed in U.S. Pat. No. 4,795,855. Further, U.S. Pat. No. 4,940,838 to Schilperoort et al. discloses a binary Agrobacterium vector (i.e., one in which the Agrobacterium contains one plasmid having the vir region of a Ti plasmid but no T-DNA region, and a second plasmid having a T-DNA region but no vir region) useful in carrying out the present invention. Microparticles carrying a DNA construct of the present invention, which microparticle is suitable for the ballistic transformation of a plant cell, are also useful for making transformed plants of the present invention. The microparticle is propelled into a plant cell to produce a transformed plant cell and a plant is regenerated from the transformed plant cell. Any suitable ballistic cell transformation methodology and apparatus can be used in practicing the present invention. Exemplary apparatus and procedures are disclosed in Sanford and Wolf, U.S. Pat. No. 4,945,050, and in Agracetus European Patent Application Publication No. 0 270 356, titled �Pollen-mediated Plant Transformation�. When using ballistic transformation procedures, the expression cassette may be incorporated into a plasmid capable of replicating in the cell to be transformed. Examples of microparticles suitable for use in such systems include 1 to 5 μm gold spheres. The DNA construct may be deposited on the microparticle by any suitable technique, such as by precipitation. Plant species may be transformed with the DNA construct of the present invention by the DNA-mediated transformation of plant cell protoplasts and subsequent regeneration of the plant from the transformed protoplasts in accordance with procedures well known in the art. 5. Plants for Transformation and Propagation of Transformants. Plants that may be used to carry out the present invention are typically vascular plants (including angiosperms and gymnosperms, monocots and dicots). Cells used to carry out the present invention may be vascular plant cells, which may reside in vitro or in vivo in a plant tissue or intact plant, but other cell types such as bacterial cell may be employed to carry out intervening steps involved in preparing the DNA constructs employed in carrying out the present invention. A transformed plant or host cell is a plant or host cell which has been transformed or transfected with DNA constructs as disclosed herein, using recombinant DNA techniques such as those described above coupled with propagation techniques such as those described below. The promoter sequences disclosed herein may be used to express a heterologous DNA sequence in any plant species capable of utilizing the promoter (i.e., any plant species the RNA polymerase of which binds to the promoter sequences disclosed herein). Examples of plant species suitable for transformation with the DNA constructs of the present invention include both monocots and dicots, and include but are not limited to tobacco, soybean, potato, cotton, sugarbeet, sunflower, carrot, celery, flax, cabbage and other cruciferous plants, pepper, tomato, citrus trees, bean, strawberry, lettuce, maize, alfalfa, oat, wheat, rice, barley, sorghum and canola. Thus an illustrative category of plants which may be transformed with the DNA constructs of the present invention are the dicots, and a more particular category of plants which may be transformed using the DNA constructs of the present invention are members of the family Solanacae. Any plant tissue capable of subsequent clonal propagation, whether by organogenesis or embryogenesis, may be transformed with a vector of the present invention. The term �organogenesis,� as used herein, means a process by which shoots and roots are developed sequentially from meristematic centers; the term �embryogenesis,� as used herein, means a process by which shoots and roots develop together in a concerted fashion (not sequentially), whether from somatic cells or gametes. The particular tissue chosen will vary depending on the clonal propagation systems available for, and best suited to, the particular species being transformed. Exemplary tissue targets include leaf disks, pollen, embryos, cotyledons, hypocotyls, megagametophytes, callus tissue, existing meristematic tissue (e.g., apical meristems, axillary buds, and root meristems), and induced meristem tissue (e.g., cotyledon meristem and hypocotyl meristem). 6. Uses of the Invention. The present invention may be used in the manner described in U.S. Pat. No. 5,750,386 to Conkling et al. or U.S. Pat. No. 5,589,622 to Gurr et al. Thus, the present invention provides a method of controlling nematodes, comprising: (a) providing a root knot nematode-responsive promoter as described above, (b) preparing a construct as described above by combining said promoter with a further region which codes for a product disruptive of nematode attack, and (c) transforming plants with the construct to obtain plants which are root knot nematode resistant. The plants employed may be as described above, and transformation may be carried out as described above. Once a first generation (Fo generation) of transformed plants are obtained, plant seed that contains the aforesaid construct, and that germinates into a root knot nematode resistant transgenic plant, may be be produced from the Fo plants by conventional breeding procedures. An agricultural field infected with root knot nematodes, or susceptible to root knot nematode infection, can then be planted with a crop of such plants in accordance with standard techniques (e.g., by planting seed or plantlets) to provide an agricultural field of crop plants that are resistant to root knot nematode infection. The present invention is explained in greater detail in the following non-limiting Examples. Tobacco seed containing the Arabidopsis cell promoter fused to and driving a GUS gene, previously described in Z. Shani et al., Plant Molec. Biol. 34, 837�842 (1997), were provided by Dr. Oded Shoseyov. Plants were grown from the seed and infected with the tobacco cyst nematode. The cel1-GUS transgenic tobacco roots did not exhibit GUS staining other than in the elongation zone of the root tips. However, it was also found that plants infected with the root knot nematode (Meloidogyne incognita), which also parasitizes tobacco, specifically upregulated cel1-GUS in specialized feeding sites called giant cells around which galls form on the roots. See The foregoing is illustrative of the present invention, and is not to be construed as limiting thereof. The invention is defined by the following claims, with equivalents of the claims to be included therein. Patent Citations
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Song details | |
Title | It's O.K. |
Artist | Fat Joe |
Album | Jealous Ones Still Envy (J.O.S.E.) [2001], Track 14 |
Genre | |
Duration | 00:03:22 |
Rank | − (−) history » |
Charts | − view all » |
Referring urls | view all » |
Song lyrics |
Yeah, right about now
I'm about to slow it up For that very special lady I see you right there But we about to smooth it out for you Right now Never, Hey, Hey, Hey, Hey, Hey, Hey! [Chorus] It's okay, it's okay It's okay, hey, we was home anyway It's alright, it's alright High, High, we was all more night NIGGAS! Tryin to come in my I'm talkin 'bout NIGGAS! Tryin to come in my Who? Who? NIGGAS! Tryin to come in my home Take my chrome, I say yo "It's On" If my house, I-I been here(speakin spanish too) Es me casa y yo viva aqui [Verse 1] Yeah, check me out now The other day I was chillin In the crib with two women We just finished swimmin Now I'm ready to slid up in 'em They the horny type, bout to get it on With the only dykes, now I hear the alarm I'm like, "Holy Christ!" Is somebody tryin to come up in my crib? I'm like "Who the fuck is this?" like Notorious Big, he wore a black suit With a black mask that match I'm bout to blast his mask off Push his cabbage back Make spaghetti out his brain Cuz I'm steady with the aim Niggas comin sideways, gettin petty wit da game Musta heard about the half in the safe The stash in the base, iced out medals in the case Niggas comin wit da chrome Tryin to sneak up in my home, rumors out I spent a Mil' on the pump piece alone I got the heat in my palm Nobody's seein it though Step your feet on my lawn I'm puttin 3 to ya dome! [Chorus] [Verse 2] Yeah, verse 2, yo Now I got these bitches Screamin fo' they life Peein on they nice Gotta funny feelin that They teamin on the heist Scheemin on the ice Wont get you nothin but killed Front if you will, get chopped up Stuffed in the quill Back to the lab, got these niggas After my math, these hoes try to play it off But they captain is back They must have teamed up With some niggas thinkin they sweet Like I just rapped and I got a ho waitin in the street I'm playin for keeps, I see 'em Creepin on the moniters Got my temperature risin faster than thermometers I burn banana clips, make all My victories unanimous I'm accurate, once I catch a peak Hey man, yo ass is hit! Now I see 'em creepin through the front door I think not! We exchange shots Like cops into getting blocked{*gunshot*} He busts, I bust back{*gunshot*} He caught one in the chest The other two got hit up In the stomache and neck{*gunshots*} I'm under the desk, freeloading Puttin slugs in the rest Wonderin what possessed these niggas To come in my rest!{*gunshots*} {*breathing hard*} You motherfuckers want a war with me? Dont you know I fuckin kill niggas? Here I come!{*gunshots*} Oh, shit! Motherfuckers is dead already! Yo, where them bitches go? submitted by Caitlin
written by Fulsom, Lowell / Freeze, Louis M. / Muggerud, Larry / Reyes, Senen / Cartagena, Joseph Anthony / Matthews, Sean / Mccracklin, Jimmy copyright with Lyrics © Universal Music Publishing Group, Royalty Network, Bug Music |
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text:
Korean-Japanese grocery store with a wide variety of products from both countries.
You can find practically anything if you're after Korean ingredients.
It's situated quite far from the city center, but easily accessible via public transport.
(The website/webshop is in Hungarian only.)
Where do you buy your Korean cooking ingredients?
Add your local store to the Korean grocery store directory and help others find good places to shop!
We have shops in many countries including Australia, Austria, Belgium, Canada, Chile, Indonesia, Italy, Spain, UAE, the UK, the USA and online.
Latest Korean grocery stores:
In Malmö, Sweden -
Nakwon Korean grocery store in Vienna Austria
In Vienna, Austria -
M2M Sheppard
In North York, Canada


text:
Music After Hours
Fri, Aug 30, 2013
Times: 5:00 p.m to 8:00 p.m
Venue: The Mount
2 Plunkett St., Lenox, MA 01240
Contact:0 people saved this
A rousing blend of traditional and modern music with influences from the Americas and beyond, performed by the region's best musicians. Relax on the terrace with a glass of wine while savoring the sounds and the view.
Salem is Oh-So-Sweet in February
Winter is about to get a whole lot sweeter in Salem. For the past decade,...
Search All Listings Here.


text:
Dr. Anne-Marie Lee, MD
Endocrinology, Diabetes & Metabolism, Allina Medical Clinic
- 303 Catlin St
Buffalo, MN 55313 - 1 Office Location
- (763) 684-7747
Dr. Lee works in Buffalo, MN and specializes in Endocrinology, Diabetes & Metabolism. Dr. Lee is affiliated with Buffalo Hospital. Dr. Lee speaks English, Russian and Spanish.
Gender: Not Available
Years in Practice: Not Available
Languages Spoken: English, Russian, Spanish
Medical School: Not Available
Graduation Date: Not Available
Hospital Affiliations: Buffalo Hospital
Specialties: Endocrinology, Diabetes & Metabolism


text:
:lol: Did you forget to change the name of the pic?
I won't say it cos i didn't know what it was at first.
I won't say it cos i didn't know what it was at first.
~*Guess the City*~napki 03-07-2006, 04:49 PM :lol: Did you forget to change the name of the pic? I won't say it cos i didn't know what it was at first. Suzi 03-07-2006, 05:12 PM lol im so retarded yup changed it :p NINA_BCN 03-07-2006, 06:14 PM Hong Kong? Suzi 03-07-2006, 06:31 PM wud i make it that obvious :ras: tut tut :p 16681 03-07-2006, 06:48 PM New York City NINA_BCN 03-07-2006, 07:09 PM wud i make it that obvious :ras: tut tut :p :nerner: Suzi 03-07-2006, 09:10 PM :nerner: Mae right country Sandra 03-07-2006, 09:16 PM L.A? shotgun 03-07-2006, 09:17 PM nice thread. :lol: Btw, Suzi, you should have started this in the non-tennis subforum. Few people will actually see it here. Laura12 03-08-2006, 12:27 AM Seattle? jackieglover 03-08-2006, 12:29 AM Chicago Lou_Lou_Ferrero 03-08-2006, 01:39 AM nuvver one please....;p El Legenda 03-08-2006, 01:54 AM http://img164.imageshack.us/img164/1905/untitled9lx1.th.png (http://img164.imageshack.us/my.php?image=untitled9lx1.png) Suzi 03-08-2006, 03:02 PM nice thread. :lol: Btw, Suzi, you should have started this in the non-tennis subforum. Few people will actually see it here. yeah i know :p but i thought peeps wud shout at me for spamming or stuff but heh :) Suzi 03-08-2006, 03:04 PM http://img164.imageshack.us/img164/1905/untitled9lx1.th.png (http://img164.imageshack.us/my.php?image=untitled9lx1.png) St Louis ;) p.s. mine was michigan :ras: Suzi 03-08-2006, 03:08 PM Ok ive changed the pic, see first pg sigmagirl91 03-08-2006, 03:13 PM Somewhere in South America...maybe Rio? Suzi 03-08-2006, 03:16 PM Somewhere in South America...maybe Rio? Nope no where near :p Hola Mr. SK 03-08-2006, 03:23 PM Amsterdam Suzi 03-08-2006, 03:37 PM Clue: Southern Hemisphere shotgun 03-08-2006, 03:40 PM Cape Town! Suzi 03-08-2006, 03:42 PM :yeah: well done heres a :hug: for you :p next pic is up shotgun 03-08-2006, 04:01 PM somewhere in Europe. :p Suzi 03-08-2006, 04:08 PM not good enough :p napki 03-08-2006, 04:19 PM Somewhere in Spain or Portugal. :p Suzi 03-08-2006, 05:18 PM City please :angel: Nikki♥ 03-08-2006, 05:30 PM It can't be Madrid. :scratch: Suzi 03-08-2006, 06:21 PM your right it isnt, keep trying :p Nikki♥ 03-08-2006, 06:39 PM But it's in Spain, right? Suzi 03-08-2006, 06:59 PM :yeah: shotgun 03-08-2006, 07:39 PM Sevilla? Suzi 03-08-2006, 07:43 PM ahhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh hhhhhhhhhhhhhhh it moved hehe hehe hehe (well i did request it heh) Thanks mods :yeah: and yeah ur right!! NINA_BCN 03-08-2006, 07:44 PM Sevilla? Si. ;) shotgun 03-08-2006, 07:49 PM :yippee: :yippee: :yippee: shotgun 03-08-2006, 07:49 PM these North-American cities are all the same. :ras: Suzi 03-08-2006, 07:52 PM :aplot: :p Lou_Lou_Ferrero 03-08-2006, 07:55 PM can i post one? NINA_BCN 03-08-2006, 07:55 PM :yippee: :yippee: :yippee: Add a pic. ;) shotgun 03-08-2006, 07:55 PM :scratch: Although it can be South-Eastern Asia as well. :p napki 03-08-2006, 07:56 PM This looks like southeastern Asia to me, and yes they're all the same :lol: shotgun 03-08-2006, 07:57 PM Add a pic. ;) Suzi has already posted one, in the original post. :p Suzi 03-08-2006, 07:57 PM can i post one? after someones got mine first then i will add urs :p NINA_BCN 03-08-2006, 07:58 PM Singapore? Suzi 03-08-2006, 07:58 PM Suzi has already posted one, in the original post. :p u tell her, alicia alwasy getting up to no good :rolleyes: bad influence she is she will need u astray :p :lol: Suzi 03-08-2006, 07:58 PM Singapore? :bigclap: NINA_BCN 03-08-2006, 08:00 PM :bigclap: :haha: shotgun 03-08-2006, 08:01 PM Okay, who will post one now? Suzi 03-08-2006, 08:02 PM :nerner: anyone wanna post one or shall i carry on..... Lou_Lou_Ferrero 03-08-2006, 08:03 PM do you want to keep all the pics on the first page? Suzi 03-08-2006, 08:04 PM yup so its easy for newbies Suzi 03-08-2006, 08:04 PM but u can post one and then i repost it in the first page as well Lou_Lou_Ferrero 03-08-2006, 08:06 PM ok.... so how do i post on the frist page? shotgun 03-08-2006, 08:06 PM Guess. Lou_Lou_Ferrero 03-08-2006, 08:06 PM but u can post one and then i repost it in the first page as well AHA! So simple.... ok http://img.photobucket.com/albums/v701/lottie_doyle/M02.jpg Sandra 03-08-2006, 08:07 PM its harder on the first page :p you post it both here and there okay? :p Suzi 03-08-2006, 08:08 PM i cant see urs lous anyways im off out so happy gaming and tell me who won wot cos i need to updat the league tables to see who will be king or queen loz Sandra 03-08-2006, 08:09 PM I can't guess anything. I need clues :p shotgun 03-08-2006, 08:09 PM AHA! So simple.... ok Probably Spain or southern Italy. Suzi 03-08-2006, 08:09 PM i can see it now :p Sandra 03-08-2006, 08:10 PM Napoli?:p Suzi 03-08-2006, 08:12 PM Milan Lou_Lou_Ferrero 03-08-2006, 08:13 PM nope..... all wrong.... Sandra 03-08-2006, 08:13 PM Palermo Lou_Lou_Ferrero 03-08-2006, 08:14 PM clue: it's not in spain or italy i LOVE THIS!! THE POWER!! Sandra 03-08-2006, 08:14 PM Malaga? Mallorca? Sandra 03-08-2006, 08:14 PM Ok then don't count mine :p shotgun 03-08-2006, 08:15 PM lisbon? Sandra 03-08-2006, 08:15 PM Nice? Lou_Lou_Ferrero 03-08-2006, 08:15 PM clue: it's not the usual scene that you would associate with the city- this pic is of the old part of the city... shotgun 03-08-2006, 08:16 PM paris? Lou_Lou_Ferrero 03-08-2006, 08:16 PM Nice? GIVE THE GIRL A CHOCOLATE-COVERED JUANQUI!! It is Nice Suzi 03-08-2006, 08:16 PM :ras: im back not going till 9 cos footies on, damn them!! Suzi 03-08-2006, 08:17 PM damn u sandee beeting me at my own game ...... im posting now Sandra 03-08-2006, 08:19 PM GIVE THE GIRL A CHOCOLATE-COVERED JUANQUI!! It is Nice :banana: I'm good :cool: ok next one :p Suzi 03-08-2006, 08:21 PM http://i23.photobucket.com/albums/b367/Suzi87/blah.jpg Sandra 03-08-2006, 08:21 PM Come on Suz :p I like this game :angel: Lou_Lou_Ferrero 03-08-2006, 08:22 PM god i suck at this game.... somewhere is Australia? Melbourne?! Sandra 03-08-2006, 08:23 PM http://i23.photobucket.com/albums/b367/Suzi87/blah.jpg Suzi I don't like your cities :lol: Kuala Lumpur? :p Suzi 03-08-2006, 08:23 PM not close both of ya Suzi 03-08-2006, 08:24 PM u really think im gonna be easy on ya :p clue: its a capital Sandra 03-08-2006, 08:24 PM Tell us the continent :p Lou_Lou_Ferrero 03-08-2006, 08:25 PM delhi? Suzi 03-08-2006, 08:25 PM Its not Australasia or Asia Lou_Lou_Ferrero 03-08-2006, 08:25 PM Brasilia? Suzi 03-08-2006, 08:25 PM hurry up i have another one lol Sandra 03-08-2006, 08:25 PM Phom-Phen? :p Suzi 03-08-2006, 08:26 PM Not S.America Suzi 03-08-2006, 08:26 PM Phom-Phen? :p wot part of not in Asia didnt u get :p :lol: Lou_Lou_Ferrero 03-08-2006, 08:26 PM washington??!! *grasps at straws* Suzi 03-08-2006, 08:27 PM OK last clue its not in the EU Sandra 03-08-2006, 08:28 PM Ok then mine doesnt count :p London? :p Sandra 03-08-2006, 08:28 PM Suz you are too afst for my comp :p Suzi 03-08-2006, 08:29 PM Ok then mine doesnt count :p London? :p Ok wot part of not in the EU didnt u get :p :lol: deja vu shotgun 03-08-2006, 08:29 PM mexico city? Suzi 03-08-2006, 08:29 PM nope Sandra 03-08-2006, 08:29 PM What part of my other post didnt you get? :p napki 03-08-2006, 08:29 PM Moscow? alelysafina 03-08-2006, 08:30 PM Chicago? Lou_Lou_Ferrero 03-08-2006, 08:30 PM washington dc Sandra 03-08-2006, 08:30 PM Kiev? Minsk? Suzi 03-08-2006, 08:30 PM What part of my other post didnt you get? :p well after i post it i read it cant be bothered to edit now :ras: and Napki oooooo getting warmer ut still wrong country shotgun 03-08-2006, 08:30 PM ottawa Lou_Lou_Ferrero 03-08-2006, 08:32 PM bucharest? alelysafina 03-08-2006, 08:32 PM vancouver? Sandra 03-08-2006, 08:32 PM no :p it cant be Lou_Lou_Ferrero 03-08-2006, 08:33 PM zagreb? alelysafina 03-08-2006, 08:33 PM Im just throwing names out there :p Are you sure it's not chicago, illinois? Lou_Lou_Ferrero 03-08-2006, 08:33 PM sofia? Lou_Lou_Ferrero 03-08-2006, 08:33 PM Im just throwing names out there :P me too!! :rolleyes: Sandra 03-08-2006, 08:33 PM Belgrade? shotgun 03-08-2006, 08:33 PM EU = Europe? alelysafina 03-08-2006, 08:34 PM question, after someones gets this one can I post one?? alelysafina 03-08-2006, 08:35 PM alberta? Sandra 03-08-2006, 08:35 PM EU = Europe? European Union Suzi 03-08-2006, 08:35 PM sure u can Suzi 03-08-2006, 08:36 PM that answer was to alelysafina :p alelysafina 03-08-2006, 08:36 PM another clue??? napki 03-08-2006, 08:36 PM Oslo? :p Sandra 03-08-2006, 08:36 PM so no one guessed yet? Tallin, Riga or Villnius? Suzi 03-08-2006, 08:38 PM God :o ok ummmmmmmmmmmm its borderd by 7 countries and the B***** Sea (im talking about the country not the city but i want the city and i know i am waffling so :p ) shotgun 03-08-2006, 08:38 PM tbilisi Sandra 03-08-2006, 08:38 PM Helsinki? Copenhagen? Stockholm? These are nto good, EU :p shotgun 03-08-2006, 08:38 PM minsk Suzi 03-08-2006, 08:38 PM one guess per post sandra thats just cheating :p better make some rules up lol shotgun 03-08-2006, 08:38 PM warsaw alelysafina 03-08-2006, 08:39 PM Portland? Sandra 03-08-2006, 08:39 PM I said Minsk :p Suzi 03-08-2006, 08:39 PM Helsinki? Copenhagen? Stockholm? These are nto good, EU :p they are in the EU!!! :p Suzi 03-08-2006, 08:40 PM warsaw :yeah: shotgun 03-08-2006, 08:40 PM :woohoo: Sandra 03-08-2006, 08:40 PM one guess per post sandra thats just cheating :p better make some rules up lol So i guessed in any of my multiple choice posts? Suzi 03-08-2006, 08:41 PM haha so whos posting or am i carrying on till i leave :p alelysafina 03-08-2006, 08:41 PM You are too good shotgun blues :worship: shotgun 03-08-2006, 08:41 PM can I post one? alelysafina 03-08-2006, 08:41 PM I want to post please Sandra 03-08-2006, 08:41 PM Ok next one :mad: Suzi 03-08-2006, 08:42 PM ok i think i said i let alelysafina then shotgun u can post after wards Suzi 03-08-2006, 08:45 PM ANYONE!! :o shotgun 03-08-2006, 08:46 PM Well I had one ready, but since it's not my turn... :shrug: Suzi 03-08-2006, 08:47 PM ok shove one on then alelysafina 03-08-2006, 08:48 PM Take my turn, shotgun blues, my laptop is running out of batteries and I think it might turn off soon, so I'll just post one later :) Suzi 03-08-2006, 08:49 PM :hug: Suzi 03-08-2006, 08:49 PM if not i will do it :p alelysafina 03-08-2006, 08:50 PM :hug: :wavey: bye guys, I'll come on and play later :) Suzi 03-08-2006, 08:50 PM :wavey: bye :hug: Sandra 03-08-2006, 08:51 PM Anyone? NINA_BCN 03-08-2006, 08:51 PM C'mon Suzi! ;) Suzi 03-08-2006, 08:51 PM http://i23.photobucket.com/albums/b367/Suzi87/Hoot.jpg Sandra 03-08-2006, 08:53 PM http://i23.photobucket.com/albums/b367/Suzi87/Hoot.jpg Toronto? NINA_BCN 03-08-2006, 08:53 PM Ottawa :p Suzi 03-08-2006, 08:54 PM nope Sandra 03-08-2006, 08:57 PM Vancouver Suzi 03-08-2006, 08:59 PM yup :yeah: ok im off for real now u guys carry on playing but it wont count cos im not here to over see things haha (well i cant be arsed to add things and stuff so bye) Sandra 03-08-2006, 09:01 PM I'm good :woohoo: More pics? Carlita 03-08-2006, 09:06 PM I got one :) way to easy though :lol: Sandra 03-08-2006, 09:07 PM Not for me :lol: Madrid? Carlita 03-08-2006, 09:08 PM nope Lou_Lou_Ferrero 03-08-2006, 09:10 PM i really recognise it but.... is it in south amaerica? Lou_Lou_Ferrero 03-08-2006, 09:12 PM *sigh* i have no idea Sandra 03-08-2006, 09:13 PM Melb? Carlita 03-08-2006, 09:13 PM go on! THINK GIRLS!! :lol: Sandra 03-08-2006, 09:14 PM brisbane? Lou_Lou_Ferrero 03-08-2006, 09:14 PM indian wells Lou_Lou_Ferrero 03-08-2006, 09:15 PM is Indian Wells a city?!!!! Geography was never my strong point at school.... Carlita 03-08-2006, 09:16 PM yeah indian wells is a city....I think....though this is not it :haha: and NO to Brisbane as well:p Lou_Lou_Ferrero 03-08-2006, 09:16 PM dubai Carlita 03-08-2006, 09:16 PM :spit: noooooooooo hahahahaha Lou_Lou_Ferrero 03-08-2006, 09:16 PM give us a clue?! Sommarsverige 03-08-2006, 09:18 PM I go into a different direction and say Johannesburg Carlita 03-08-2006, 09:18 PM it's NOT in Australia Sandra 03-08-2006, 09:19 PM Nairobi? napki 03-08-2006, 09:20 PM Buenos Aires? Carlita 03-08-2006, 09:20 PM no okay, look in Europa :) Lou_Lou_Ferrero 03-08-2006, 09:20 PM Rio??!!!!!!! dear god Sandra 03-08-2006, 09:21 PM Toronto? Lou_Lou_Ferrero 03-08-2006, 09:21 PM somewhere in holland???? Sommarsverige 03-08-2006, 09:21 PM okay, next guess: Lisbon? Sandra 03-08-2006, 09:21 PM Barcelona? shotgun 03-08-2006, 09:21 PM mallorca? Carlita 03-08-2006, 09:22 PM keep guessing :dance: NINA_BCN 03-08-2006, 09:23 PM The bridge...:scratch: Lou_Lou_Ferrero 03-08-2006, 09:23 PM erm. europe.... europe europe europe..... NINA_BCN 03-08-2006, 09:23 PM Valencia? Carlita 03-08-2006, 09:24 PM nope :haha: i like this game Lou_Lou_Ferrero 03-08-2006, 09:24 PM hamburg Sandra 03-08-2006, 09:25 PM :mad: Manchester? NINA_BCN 03-08-2006, 09:25 PM London???????? Lou_Lou_Ferrero 03-08-2006, 09:26 PM newcastle? Carlita 03-08-2006, 09:26 PM No, no and no :haha::spit: Sommarsverige 03-08-2006, 09:26 PM hamburg No, I am very sure this definitely isn´t Hamburg ;) But London could be right in my eyes, too! Lou_Lou_Ferrero 03-08-2006, 09:27 PM liverpool Sommarsverige 03-08-2006, 09:27 PM I think you must give us some tips, Europe is just too big ;) Carlita 03-08-2006, 09:27 PM no it's not London. the bridge is called: the Swan NINA_BCN 03-08-2006, 09:27 PM Sevilla again??? Sandra 03-08-2006, 09:27 PM Frankfurt? NINA_BCN 03-08-2006, 09:29 PM Stockholm?? shotgun 03-08-2006, 09:29 PM cardiff? Lou_Lou_Ferrero 03-08-2006, 09:30 PM CARDIFF NINA_BCN 03-08-2006, 09:30 PM Vienna? Lou_Lou_Ferrero 03-08-2006, 09:30 PM St Petersburg Carlita 03-08-2006, 09:31 PM it's not in the south of Europe shotgun 03-08-2006, 09:31 PM dublin shotgun 03-08-2006, 09:32 PM belfast NINA_BCN 03-08-2006, 09:32 PM Helsinki shotgun 03-08-2006, 09:32 PM rotterdam NINA_BCN 03-08-2006, 09:33 PM Oslo Sandra 03-08-2006, 09:33 PM Helsinki? Sandra 03-08-2006, 09:34 PM Fuck my comp is too slow for this game :mad: Carlita 03-08-2006, 09:35 PM rotterdamhttp://img2.menstennisforums.com/500/thumbs/winner_first_h4h.gif :bigclap: well done NINA_BCN 03-08-2006, 09:35 PM Reykjavik? *edit* :ras: shotgun 03-08-2006, 09:36 PM :D Sommarsverige 03-08-2006, 09:37 PM I think always the person who gets the right answer, should post the next pic, or? Sandra 03-08-2006, 09:37 PM Tallin? shotgun 03-08-2006, 09:38 PM I think always the person who gets the right answer, should post the next pic, or? Seems a good rule to me. Wait a minute and I'll post a pic. shotgun 03-08-2006, 09:40 PM Okay, guess. Carlita 03-08-2006, 09:41 PM buenos aires Sommarsverige 03-08-2006, 09:41 PM Buenos Aires? Lou_Lou_Ferrero 03-08-2006, 09:41 PM looks like nroth spain to me.... is it Bilbao? shotgun 03-08-2006, 09:42 PM buenos aires Right continent, wrong country. :p Lou_Lou_Ferrero 03-08-2006, 09:42 PM everyone else is going for buenos aries.... Sandra 03-08-2006, 09:42 PM Montevideo? Carlita 03-08-2006, 09:43 PM Santiago de Chile? Lou_Lou_Ferrero 03-08-2006, 09:43 PM lima ok so i have no idea.. is it that obvious?! shotgun 03-08-2006, 09:43 PM nope Sandra 03-08-2006, 09:43 PM La Paz? Sandra 03-08-2006, 09:43 PM Asuncion? shotgun 03-08-2006, 09:44 PM think about the mountains with the iced peak. shotgun 03-08-2006, 09:44 PM La Paz? Correct! Sandra 03-08-2006, 09:44 PM somewhere cold :p Carlita 03-08-2006, 09:45 PM that was a quick one :lol: Lou_Lou_Ferrero 03-08-2006, 09:45 PM where now?!!!! La Paz???? where is that?! Sandra 03-08-2006, 09:45 PM Correct! I am so good :lol: :cool: shotgun 03-08-2006, 09:45 PM where now?!!!! La Paz???? where is that?! Bolivia. :lol: In the middle of the Andes. Sandra 03-08-2006, 09:56 PM Easy one :p Carlita 03-08-2006, 10:04 PM easy??? okay...if you say so :lol: jmgsdoubsprtnr 03-08-2006, 10:36 PM uhhh, stockholm? just a wild guess... :p Lou_Lou_Ferrero 03-08-2006, 10:59 PM somewhere in denmark? Suzi 03-09-2006, 12:11 AM easy my arse :ras: Suzi 03-09-2006, 12:13 AM Romania Carlita 03-09-2006, 06:53 AM no it's not, keep guessing :p Sommarsverige 03-09-2006, 07:51 AM Ontario? Sandra 03-09-2006, 08:12 AM No. Sandra 03-09-2006, 08:16 AM It's more known than La Paz, and it is not in Europe. Suzi arent you gonna count the right picks we had last night?:p Suzi 03-09-2006, 10:07 AM Nope Suzi 03-09-2006, 10:09 AM Ottawa Sandra 03-09-2006, 10:10 AM Why not? :bigcry: No Southern Emisphere Suzi 03-09-2006, 10:11 AM cos theres too many pages to scan, if u tell me who won wot and wot cities they were they yes! Wellington Sandra 03-09-2006, 10:15 AM No, but you're close. Different country. La Paz- Me :angel: Rotterdam- Shotgun Blues :p Suzi 03-09-2006, 10:16 AM Perth Sandra 03-09-2006, 10:17 AM No, go a bit to the East. |


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WOW! Great people and ideas for me!
I have always been poly, but didnt know it had a name. I have been married to my wonderful husband for 30 years and we started mono but became poly after I wanted to reconnect with an old boyfriend. We have all been neighbors and friends for all these years without any major problems. As I have read most of the threads, I totally agree to be be honest and talk about problems and then work them out together. We moved from our familiar hometown to a larger city and I was wondering how to find another friend. I found one and he is so mono, but learning about this way of loving and living. I guess we have a vee, with me being the hinge. How nice to hear of others lives and how similiar we live and love. We now both have friends to love and be with. Love hearing about red pepper and her two beloved men. I feel a connection with her already. Her advice has really resonated with me and my new situation. Thanks for being a place to learn and feel accepted. My work would not understand so I must be hidden. My adult children know about my friend and accept love as something precious to be given and to give away. They are very wonderful sons. Thanks for a great sounding board. |
Welcome! Sounds like you (the plural you) have got off to a good start, and made things work that many couples stumble over for a long time. Best wishes, and welcome again!
Mono |
Welcome. It looks like you are starting well.
Welcome to the boards. Where in Alaska were you from?
We are in Alaska and there is another poster who was from Alaska too. :) I like that we were able to actually find people who have some idea of our daily life on here! I expected to find people who were poly (duh) but it's sometimes hard to find people who know what LIFE is like in Alaska. It's pretty isolated from the rest of the world and hard to find people who are into poly for instance without getting into people you know from work. For example, I searched for poly communities in Ak and found one "list of interested people in Ak if one starts" of the 30 something people I knew 4 and my husband knew 2 others, our 3rd knew 4 more and a close friend of mine personally knew 2-3 . So between us we realized there was no chance of participating in something like that HERE and keeping anonymity which honestly would be very important for my husbands job as well (though not mine or C's). ANYWAY-welcome and nice to "meet" you! |
WOW there really are other polyfolk in Alaska!!!
I was beginning to think we were on an Island!
(that is a word from my son, when he was learning to talk and we asked him a question he always said "nope and I wanna..." as he got a little bigger it was "Nope-in I wanna", now we all say nopin) |
All times are GMT. The time now is 02:21 AM. |


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The Enchanted Cave 2
Delve into a strange cave with a seemingly endless supply of treasure, strategically choos
4.31 / 5.00 28,393 ViewsGhostbusters B.I.P.
COMPLETE edition of the interactive "choose next panel" comic
4.00 / 5.00 7,858 Views***LOWER QUALITY IS RECOMMENDED FOR THE BEST SEXY DANCING***
This game is finally done! A collaboration between BlueHippo(game maker) and Dave(dancer)! Dave came up with the concept, when he found a rusty (jk ACC) version of this game, and we decided to touch it up, and add some fat nudity! Aw yeah!
It's quite simple! All you do is press the key on the keyboard, and he will perform a dance move! You can change the song and the background, too! Make Dave do whatever you want, it's quite addicting ;)
Songs all stated in credits. Be sure to have lots of fun! And vote five! oh yeah, and go to www.colonthree.com ;)
Man this was fun. :D Catchy tunes, and that's a ton of funky dance moves! Feels like theyäre all so easy to sync to, either let them run or repeatedly swap keys to add whatever patterns you want, love the idea, and the results lmao. Genius!
If there was a recording feature, this could have been a very highly amusing game! Well done!


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hello again!i am very intersted in finding family, but i have very little information to offer you, i am currently not in much contact with my father, and he would be the only one i know who might know who his father's parents were, but he has recently tried to contact me, so i will ask him for as much information as possible when i do speak with him, is there any thing else i should ask? Notify Administrator about this message?
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Video: Mayday Minute
November, 22, 2013
By ESPN.com staff | ESPN.com
Mark May discusses whether Ed Orgeron deserves to be USC's coach, Boston College's Andre Williams looking to join an exclusive club, Kansas gets its first conference win since Nov. 6, 2010, and Duke looking for its first nine-win season since 1941.
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Happy #MartinLutherKing Day from Marygrove College. http://t.co/AUm50LySKV
Attorneys have a variety of academic backgrounds-economics, English, history, philosophy and political science. Law schools do not designate a major program concentration as a prerequisite for admission. Law schools admit students who are broadly trained and who evidence analytical skills, good writing and oral communication abilities, as well as social awareness.
You can' therefore' prepare for law school by fulfilling the general education requirements at Marygrove College, and choosing from a variety of majors and minors.
Your academic major can help prepare you for law school. It can also provide you with training for an alternative career should you choose not to enter law school immediately after graduating from Marygrove College.
A pre-law counselor is available at Marygrove to assist you in planning your education and identifying professional opportunities. The counselor keeps abreast of trends in legal education and can assist you with law school application procedures, options for financial assistance' information on The Law School Admission Test (LSAT) and career planning.
Happy #MartinLutherKing Day from Marygrove College. http://t.co/AUm50LySKV
RT @jbrownroosevelt: Congrats Dallas Chicone of PHN signed with Marygrove to play ball! Proud @PHSchools @PHNAthletics @MGroveAthletics htt…
Marygrove College will be closed Monday, January 19th in observance of #MartinLutherKing Day. http://t.co/1lOSJoTZTx
.@MarygroveDance's Marathon fundraiser is going on RIGHT NOW until midnight in LA 211! Stop by & move your body! http://t.co/cyFcACjs7O
RT @PHNHuskyBasebal: Congratulations is in order for Dallas Chicone on officially signing with Marygrove College. Manning the hot corner. h…


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Source: Lower Egypt, Thebes and the Pyramids. / F. Frith.
Citadel (Cairo, Egypt)
Frith, Francis
Gates -- Egypt -- Cairo
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The Middle East in Early Prints and Photographs
Stephen A. Schwarzman Building / Photography Collection, Miriam and Ira D. Wallach Division of Art, Prints and Photographs
All Any
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The Báb El-Azab Citadel, Cairo ([1862?])
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VX-23 SALTY DOGS! by Dave - about 3 years ago
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Jayda Leder-Luis
This whole experiment of not using my debit card has made me more aware of each cent I spend. Literally.
I always carried a few pennies and dimes in my wallet, but after a week I'd stash them away in a change jar at home. It's practically empty, considering how little I used cash in the past, but after these past two weeks I've notice substantial growth.
My bank has a "keep-the-change" policy, so that every time I use my debit card the purchase gets rounded up and the change is deposited into my savings account. While this feature is basically the electronic version of my change jar, I never noticed its effects. Seeing the physical jar containing actual money creates a mental note of all my purchases and ultimately has a bigger impact on my spending and saving habits.
Since I stopped using my debit card, not all of my change gets dumped in the jar. I use it every chance I get and frankly it's made most transactions easier.
Take the example of a $2.64 cup of coffee. I can either hand the cashier exact change, or pay $3.14 and get 50 cents back. Keeping exact change for times like these means I don't receive burdensome amounts of change back, or better yet can control how much I do get. By doing a little math each time I was at the register I was able to collect enough quarters to do a whole load of laundry. It only took me four days and didn't require a separate trip to see a bank teller.
So far there has only been one downside to using up my change. A few days ago I was in a fairly long line and it was getting longer by the minute. As I reached the counter, ordered and began to pay, I heard the gentleman behind me grumble something about wasting his time. He had his debit card out and was ready to swipe and dash out of there, but my minute-long transaction was too long for him. When I received my change I slid to the side to let him order while I put my change back in my wallet. He was done before I was, and gave me a look that screamed contempt.
Yes, cash transactions tend to take a bit longer, especially when the cashier has to count change back. It's still money, though, and using it makes a lot of sense to me.
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The Massachusetts Lemon Laws provide legal relief to consumers who are sold a new, used, or leased vehicle that has a significant defect to its safety or use. Under these laws, car dealerships are required to place a bright yellow Lemon Law sticker on each …Continue Reading Survey Says: Lemon Law Compliance on the Rise
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Dec. 12, 2013
MONMOUTH—The Carl Sandburg College women's basketball team pulled away in the second half to beat Monmouth JV 67-49 on Wednesday night.
The Chargers (6-4) led 30-26 at halftime before outscoring the Fighting Scots 37-23 in the second. Sophomore forward Destini Bollinger (Astoria, Ill./Astoria) and freshman forward Sadee Hamilton (Galesburg, Ill./Galesburg) each scored 10 points to lead Sandburg. Bollinger also added a team-leading eight rebounds.


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Scholars have been debating that question for ages, along with "If a tree falls in the forest, and no one is around, does it make a sound?" and "Why don't we see any baby squirrels?" and "What the heck is happening on ABC's Lost?" (BTW, if anyone actually knows what the heck is happening on Lost, how Sayid ends up being Ben's hitman (!), let me know -- I still believe the "island is purgatory" theory -- it certainly is for viewers -- even though it has been debunked by the show's creator. As if! I guess that makes me a Lost denier ... but I digress.) I was inspired to re-examine this age-old question after the recent remarks of the Disinformer-in-Chief in his keynote address at the Washington International Renewable Energy Conference, a ministerial-level conference hosted by the U.S. government. He said: Now, look, I understand stereotypes are hard to defeat. People get an image planted in their head, and sometimes it causes them not to listen to the facts. But America is in the lead when it comes to energy independence; we're in the lead when it comes to new technologies; we're in the lead when it comes to global climate change -- and we'll stay that way. [Applause.] Side note: The "Is it still disinformation if the speaker gets applause?" question was actually settled by Aristotle himself in his little-known book The Duh of Rhetoric.
The Western Climate Initiative is a path-breaking effort. Insufficient federal progress prompted seven states and two provinces to join together to reduce climate pollution by means of an economy-wide cap-and-trade program. It's a momentous opportunity, and many folks have been working hard to ensure that it's a success. Unfortunately, there's now cause for serious concern. Yesterday evening, WCI released its draft proposal (PDF). It proposes an initial cap that would cover less than half of the region's total emissions. Most surprisingly, WCI does not recommend including emissions from transportation fuels, by far the largest source of climate pollution in the West. [Update 3/7: The recommendation doesn't exclude transportation precisely, but rather defers the decision until further economic studies are completed.] The proposal is at odds with WCI's own stated principles that include a commitment to cover "as many emissions sources as practical." And for an effort born of frustration with federal lawmakers, it's bizarre that the proposal is significantly smaller in scope than recent federal bills (PDF), including Leiberman-Warner. There are no big technical challenges to including transportation fuels. In fact, the WCI admits that while there are a couple of hurdles, it's administratively feasible to include transportation emissions. So what's going on? No one knows for sure.
A bill introduced Thursday in the House of Representatives would grant California the right to regulate greenhouse-gas emissions from vehicles, and pave the way for 12 other states to do the same. The U.S. EPA's …
A short while ago, Sir Oolius received a fundraising email from the Competitive Enterprise Institute asking for donations to help them with their new raison d'etre: yelling "FU, Al Gore!" as loudly and as often as possible. The fruits of this effort are now upon us in the form of a national ad whining campaign: If carbon = life, then Al Gore ...
I have nothing pithy to add to this story, but only because the inanity of the quotes is so hard to top. From Restructuring Today ($ub req'd) (my emphasis on the good bits):
Okay, so at the recent Heartless Heartland skeptic/denier/disinformer/climate-destroyer conference (I promise to propose a better term this week!), one of the few attendees who was a non-non-believer in science emailed me the following: Marc Morano, Sen. Inhofe's press secretary, just cited your post on the dangers of consensus as an example of how deniers are forcing climate action proponents to retreat. "We're making them afraid of using the term 'consensus'!" Now, that is humor! After all, my article is titled "The cold truth about climate change: Deniers say there's no consensus about global warming. Well, there's not. There's well-tested science and real-world observations [that are much more worrisome]," and it explains that: "Consensus" is far too weak a word to describe the collective scientific understanding of the dangers of human-caused global warming. The reality of climate change is almost certainly going to be much worse than the "consensus" as that term is normally used (to describe the IPCC reports). The deniers are peddling pseudoscience.
So, who said: With $55 oil we don't need incentives to oil and gas companies to explore. There are plenty of incentives. Yes, that would be our president, three years ago. And yet with oil at nearly twice that price, Bush still refuses to cut subsidies and shift that money to clean technologies. And he still claims that the solution to our energy and climate problems is "technology, technology, technology, blah, blah." But, as we've seen, that is all just rhetoric or sleight of hand. Daniel J. Weiss, Director of Energy Strategy at the Center for American Progress, has an article on the urgent need for this switch in priorities: "Unbearable cost of oil: Record prices require Senate action." As Weiss points out, this will be one more chance for McCain to do the right thing:
Dingell says he'll release a draft of a House climate change bill for comment and feedback in mid-April (sub rqd).


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Primary Dance - Jazz - Tap - Ballet - Pointe - Lyrical - Hip Hop - Boy's Hip Hop -
Hippity Hop - Theater Dance
Adult Hip Hop & PiYo Classes
Artistic Director Suzanne Welch-Kakouris
Member D.T.C.B
Our annual recital will be Sunday, May 17th. More information to come in the next few months!
Registration for the 2014-2015 year has closed.
"When children enjoy themselves, they succeed. SDC is proof positive of that" - Sonia Rollins, Burlington, MA


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From the outside, the Villa looks grand and very inviting. Ingrid in the Front Office gave me a personal welcome and swift service. I was staying in one of the Villa's lifestyle rooms, which although being the standard room boasted a large bathroom and a two-storey loft. With 2 TVs I could choose to relax in the comfy chairs or... More
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Quoting kmrtigger:
Quoting psych_mom:and seriously, can't you give your kid some tylenol and wait until the doctor opens at 9:00 AM like the rest of us?
Sorry, but I have taken my oldest a few times over his ear aches(infections). He never complains or lets us know he is having them until it is too extreme and pain medicines do nothing to help. He has had 4 sets of tubes put in, and is going in again this month for his 5th set. He has very bad fluid buildup. But I do try the pain medicine before making the decision to go, and after making an appointment with his doctor(who is always booked for wks).
Has he had his adenoids or tonsils removed? My son had horrible fluid build up that went away instantly after his adenoids were removed.
You are legally entitled to take your kids to the doctor, but I know some employers pressure otherwise.
Personally, we try urgent care and minute clinics if possible before going to the ER.
You can misuse the ER by using it for non-emergencies because it costs the medical system a lot more than taking your child to the doctor. If you have an employer based healthcare plan, then you actually end up costing the employer more by raising the insurance premiums by NOT taking the time off work to take your child to the doctor during the day.
Quoting fineyouguyswin:Not everyone can afford to take off a day of work to go to the doctor. And how can you misuse the ER?
Quoting kmrtigger:That irks me when people misuse the ER. I know a person that is ALWAYS going to the ER 3-4 if not more times a month. And she does it to avoid having to take a day off work to make an appointment for the kids or herself. Pretty selfish if you ask me.
Quoting psych_mom:I can't believe that people actually run to ER over the smallest thing. When did an ear ache become an emergency?


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Menomonee Falls — After almost 25 years, the TIF district containing Village Centre has officially closed. In a way that means it has reopened for taxpayers, as the property tax revenue will now rejoin the tax base of the village.
The Village Board on April 21 voted to close the district, which was created in 1991 to promote improvements and projects in the 150-acre historic downtown area near Main Street and Appleton Avenue.
The district now has an equalized value of $48 million, which is more than double the base property value of about $22.41 million it started with in 1991. As a result of the projects that have taken place, Village Manager Mark Fitzgerald said blighted properties have been redeveloped, infrastructure investments were completed, and the number of residents and business in the downtown area have all increased.
"In today's society, downtowns are sort of fragile and they suffer from some level of disinvestment because they tend to be the mom and pop core of the community, so protecting this is a reasonable policy," Fitzgerald said.
Village President Randy Newman highlighted work in the Mill Pond area as some of the most noticeable upgrades that have occurred.
TIF is a mechanism that allows municipalities to borrow money to fund infrastructure improvements for an area that would otherwise be difficult to develop or redevelop. The increased property tax revenue from the improved land is then diverted from the tax roll to pay off the loan.
The district is closing four years earlier than anticipated, as the tax increments collected through 2014 will be more than sufficient to pay off the remaining debt service of the districts.
A $182,000 surplus will also be distributed among the affected taxing bodies, including the village, Menomonee Falls School District, Waukesha County Technical College and Waukesha County.
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Mixing seperate audio/video sources
Started by kavorka19, Jan 16 2013 09:39 AM
4 replies to this topic
#1 of 5 OFFLINE
Posted January 16 2013 - 09:39 AM
Hello everyone. I recently received a refurbished Denon AVR-1612 this past Christmas as a gift and had a quick question. I have a PS3 hooked up to it and when I play it, I like to blast some music while I play. I can't seem to figure out how to get the receiver to play the PS3 video, but play the tuner or iPod audio. Is something like this possible? If so, could someone help walk me through how to set this up? Please & thank-you!
#2 of 5 OFFLINE
Posted January 16 2013 - 10:00 AM
Connect your Ipod to a REAR audio only input. Tuner won't work and neither will the front(cause it has video).
#3 of 5 OFFLINE
Posted January 16 2013 - 10:04 AM
Didn't realize the 1612 was "that" basic. Your only hope is to connect your Ipod to "Dock" and hope it isn't "meant" to be used with a composite. (The S-video is marked dock, so maybe you can't with the 1612)
#4 of 5 OFFLINE
Posted January 16 2013 - 10:43 AM
Hi im new how do i post a new thread thi g?
#5 of 5 OFFLINE
Posted January 16 2013 - 10:23 PM
First, choose the appropriate forum (Begginers, Recievers, Speakers, etc), then click on the "Start A New Thread" tab on the upper left of the page, fill in the Title and type away. Then click on the Submit tab of course.Hi im new how do i post a new thread thi g?
"Everyday room": Panasonic 58" Plasma, Dish HD DVR, Pioneer Elite vsx-23, BDP-23 BR, dv58avi universal dvd player, Paradigm Studio 20 V1, CC-450, Dayton HSU-10 subwoofer.
"Movie/Music room": Toshiba 65" DLP, Dish HD receiver, Marantz 7005, CC-4003, BD-7006, Polk LSI25's-LSi7's-LSiC, 2 original Dayton 10" "Mighty-Mites" subwoofers. (subject to change without notice).
Also have MB Quart Vera VS05 +.....too much to list. Help me.
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