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Our new guest blogger, Christine, is here to tell us about a few hot links in Boston. For those of you outside of Boston, I'm sure you'll also enjoy these. By the way, if you missed my earlier post, learn about Christine here.
Home Stores:
Boutique Fabulous – a great little space in Somerville with mod lamps, vintage martini glasses, and yummy soaps and balms. Very unique and Sophistigirly! I've seen everything from 'Save Martha!' t-shirts to a vintage 60's party dress. Read a review about Boutique Fabulous on Boston's Weekly Dig.
Bo Concept on Mass Ave near Harvard Square.
There's also a tres cool place on Charles Street in Beacon Hill. The name is 'Good'
Sooo delish and Japanese minimalist…
Paper Source in Boston, Brookline, and Cambridge and Vellum on Dartmouth.
I also like 'Black Ink' in Harvard Square (Cambridge) and on Charles Street (Beacon Hill, Boston). Same owners as the MUT.
Another find, the Cross store in Harvard Square, surprisingly fashionable in terms of paper goods, stationery, and bags.
Exhibition must see:
There's the new exhibition at the ICA, "Living in Motion", which I haven't yet seen but have heard good things.
(above links contributed by Christine Liu. Thanks, Christine!)
Christine M. Liu, originally from Frederick, Maryland, is burning steadily into her sixth year of learning, shopping, eating, writing, and designing in Cambridge, MA. She squeezed in web-designing, pentax-wielding, and viola-playing amidst mathematics-applying at Harvard College, and is now working to develop a fashion network system as a masters student in the sociable media group at the MIT Media Lab.
Some of her current obsessions include co-producing tech-conceptual fashion shows, extolling the virtues of electronica, and knitting with the fleece of fine animals everywhere. Coos of delight are induced by the works of many favourite designers, including Issey Miyake, Tord Boontje, Eero Saarinen, Sanrio…
She is is very excited to be a guest writer for decor8, and we're happy to have her on deck. With her busy schedule, we never know when she'll pop up on decor8, so stay tuned! Look for her photo near post titles, that way, you'll easily spot the posts that are from Christine.
(bio and photo contributed by Christine Liu)
While visiting DWR in Providence last month, I felt a little weak in the knees when I saw the new Frank Gehry cloud lamp floating in the air – stunning! So much more dramatic in person than in print, I think the whole creative process involved in how they were designed motivates me even more to start looking at common objects a little differently.
This goes back again to an earlier post about pushing past your design comfort zone. Canadian born Architect Frank Gehry found a new way to look at common packing paper cups and using staples, he transformed them into a cloud lamp. Of course, the DWR light fixtures aren't made of paper, he opted for flexible, tear-resistant, fire-retardant polyester instead. Good choice. The cloud lamp may have transformed into a fireball!
Tip: If you're ever shopping at DWR in Providence, ask for Marc Gelinas, store proprietor. He's very down-to-earth, friendly, and extremely knowledgeable, which will make your entire shopping experience that much more enjoyable.
By the way, if the name Gehry sounds familiar to you, he recently designed the new Stata Center for Computer, Information and Intelligence Sciences at MIT here in Cambridge, MA. View the amazing photos of the Stata Center here. Robert Campbell, Globe writer, calls the building "a work of architecture that embodies serious thinking about how people live and work, and at the same time shouts the joy of invention." I was over there the other day peering upwards in awe. If you're local, you should bring your camera and go check it out.
Koo de Kir. Wallpaper and Travel + Leisure voted them "World's Best" for home accessories. "Improper Bostonian" honored them as "Boston's Best" home boutique three years in row. Doug Wilson from Trading Spaces even shopped there for his $50,000 room. Now it's your turn to check them out and cast your vote.
Koo de Kir is a stylish retail haven offering modern fresh homewares in chic Beacon Hill. For those of you that aren't in Boston, you can visit their online store to shop their impressive selection (not as ahhh-inspiring as the real thing since their online selection is limited). This stylish retail environment also offers interior design services, and a gift registry. You'll find merchandise that you've seen in Elle Decor, Shop etc., Breathe, Lucky, Domino, and all the other big glossies, so they're right in step with the latest trends.
I like Koo de Kir because they carry items to suit every budget, the location is excellent, and you'll always find something new to add to your favorites list. Plus, strolling along Charles Street with a latte in hand, stopping at The King + I for Thai, then hopping over to Koo de Kir for some retail therapy… it's far too perfect of a day to pass by.
Koo de Kir (Coup de Coeur), "?a strike to the heart?"
(Photos: TOP LEFT: Midsummer Light $74, Koo de Kir. All photos below also from Koo de Kir.)


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Type: Online
Study for the award of a Postgraduate Certificate in Computational Chemistry and Modelling via online distance learning.
This program is offered at multiple campus locations. View All 2 Campus Locations
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The University of Florida online forensic science program is designed to meet the needs of today's working professionals.
The Lehigh Analytical Principles of Pharmaceutical Science Certificate has been developed for employees dealing with drug
The aim of Chemistry programs at IIT is to develop chemists who are able to think creatively and critically.

subset: commoncrawl


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John Curley Center for Sports Journalism
The John Curley Center for Sports Journalism explores issues and trends in sports journalism through instruction, outreach, programming and research.
The Center emphasizes internships and hands-on experience with newspapers, magazine, electronic media, and broadcasting.
News & Opinion
Dec 15, 2014
Alumnus joins team in College of Communications
Nov 14, 2014


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Unfortunately, I am not accepting new student or postdoctoral applications at WHOI/MIT for the 2015-2016 academic year. Please do consider other members of WHOI's climate group.
Possibilities exist for students interested in radiocarbon to work on tree-ring 14C projects through the National Ocean Sciences Accelerator Mass Spectrometry Facility (NOSAMS) Graduate Student Internship Program.


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Decade Old KDE Bug Fixed
Let's make this official (129 comments)
from now on, January 18 "Thiago Macieira Appreciation Day".
Announcing: Slashdot Deals - Explore geek apps, games, gadgets and more. (what is this?)
We are sorry to see you leave - Beta is different and we value the time you took to try it out. Before you decide to go, please take a look at some value-adds for Beta and learn more about it. Thank you for reading Slashdot, and for making the site better!
Let's make this official (129 comments)
from now on, January 18 "Thiago Macieira Appreciation Day".
Requires Oracle Java (71 comments)
No, I'm not going to even try to install that piece of crap. It's a shame that after so many years the OpenJDK is still so useless. In this state of affairs, for me Java is dead and buried. In all respects.
Re:A man in the middle attack (326 comments)
That's why people keeps their diamonds in safe-deposit boxes, you fool.
Read this first (569 comments)
"A $150 Camera vs. a $5,000 Camera" http://www.kenrockwell.com/tech/150-vs-5000-dollar-camera.htm
Cool (171 comments)
but I haven't yet finished fine tuning my new and shiny Kubuntu installation.
Mikrotik (319 comments)
I'm very happy using Mikrotik (mikrotik.com) products. You can buy a ~49$ license and install their software -RouterOS, a Linux derivative- on an old PC. You can also get one of their hardware products (routerboard.com), excellent choice if you care about power consumption. I highly recommend the RB750G wich is a small SOHO router in a nice plastic case. With RouterOS you can do a whole lot of interesting things, monitoring bandwidth usage is just one of these things.
Openwall site (122 comments)
the Openwall website groans and moans imploring for a facelift. it's so poignant..
Re:The most successful trolls (383 comments)
Not so anonymous, though: http://i53.tinypic.com/faa61k.png
Qt4 + SQLite3 (702 comments)
A statically linked app written in C++, with Trolltech's Qt4.4 + SQLite3 support will do the trick. If the statically linked binary results too big, just shrink it with UPX, or install the dynamically linked dlls with the app.


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Words of Peace is an independent charitable foundation set up by people inspired by Prem Rawat's powerful and
unique message — that peace is a human necessity, that peace is possible, and that it begins with each of us.
Sharing this message of peace with the world has been Prem Rawat's life's work. He has been traveling
extensively, addressing audiences and helping to change lives for the better for over five decades.
Inspired by his work, Words of Peace produces videos, learning materials, and TV programs, and helps an
ever-expanding number of international volunteers share this unique message.
Prem Rawat received an enthusiastic welcome during his return visit to Johannesburg.
See where Prem Rawat has been addressing audiences this year.
Enjoy a huge selection of videos, from 2-minute shorts to feature length.
With the passion, dedication, and creativity of over 11,000 volunteers. Discover more…
Did you know that we have active volunteers in over 85 different countries? In fact, due to the global nature of our work, it is likely that at any time somewhere in the world a Words of Peace volunteer is contributing towards making peace a reality.
All over the world people are giving peace a chance in their communities, and so can you!


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- Mumsnet Reviews
- Product Information
Your Reviews
"Brilliant for parents wanting to gets kids in music…"
This CD is just brilliant. It is great for getting kids into Classical Music and is so hilariously funny. I is the story of Shererezade as told by Rory Bremner and Brian Blessed. My little ones love it.
Read moreLessMumsnetter anntrulove
3Number of total reviews contributed on Mumsnet3Number of Technology and entertainment reviews contributed3 reviews posted


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Edward's Home Health, Inc. filed as a Domestic For-Profit Corporation in the State of Texas on Thursday, May 3, 2012 and is approximately three years old, according to public records filed with Texas Secretary of State. The filing is currently active as of the last data refresh which occured on Tuesday, December 3, 2013.
Joe Zapata serves as the Administrator
Edward Marroquin is the Director of Edward's Home Health, Inc..
Edward Marroquin is also the registered agent for the company. Also known as a statutory or resident agent, the registered agent is responsible for receiving legal notifications regarding court summons, lawsuits, and other legal actions involving the corporate entity.
Key roles for Edward's Home Health, Inc. |
Joe Zapata
Active |
Edward Marroquin
Active |
Filing Type: | Domestic For-Profit Corporation |
Status: | Active |
State: | Texas |
State ID: | 801591459 |
Date Filed: | Thursday, May 3, 2012 |
Registered Agent | Edward Marroquin |
Comprehensive Insight Plus Report |
Learn More |
The D&B Comprehensive Insight Plus Report for Edward's Home Health, Inc. combines D&B's proprietary statistical scoring with business, payment and financial information all in one report. | |
US Company Profile Report |
Learn More |
The D&B U.S. Company Profile contains basic information such as address and telephone number, executive names and titles, line of business and SIC codes, D-U-N-S Numbers, organization status, and year and location of incorporation for Edward's Home Health, Inc.. |


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I am currently planning a 4700 sq ft house with an additional 1500-2000 sq ft basement to be finished in the future. My builder's hvac guy recommends two 3-ton units GSHP (1 for 2nd floor and 1 for first and basement) with 4 zones for $70,000 with drilling ($18,000 included in the price). On the other hand, I went out and received a quote from a much larger hvac company that also does a lot of geothermals with a proposal for two units (one 3-ton unit for the 2nd floor and one 4-ton unit for the basement and first floor) and also 4 zones with a price of $58,000 ($13,000 for drilling). Both companies are using climate master tranquility 30 and all the duct work and Honeywell thermostats. Both are doing 150 ft per ton drilling. The cheaper proposal actually already includes the desuperheater and buffer tank. My question is: Is it common for a buyer to choose his own hvac guy instead of the builders "go to guy" for that big of a price difference? My builder is a very reputable guy in my area and is very well known. He seems to be a very honest guy, but he prefers to work with someone he knows and he "can be sure they are doing it correctly". Please help, appreciate any advice |
Follow-Up Postings:
I think you should assess your own comfort level, if you would not be comfortable taking charge of this part of the project then perhaps it might be better left up to the GC. Regardless, I would suggest having a Manual J calculation performed for the heat loss/heat gain calculation and a Manual D calculation done for the duct design. The credentials of the contractors should be verified along with referrals. Since Climate Master and Carrier are essentially the same you may want to look at Carrier as well, particularly since you're considering zoning as Carrier arguably has the best zoning and controls on the market. At this stage I would recommend forgetting about price, you'll eventually come back to it and negotiate your best deal anyway. Learn as much as possible about what's being proposed, why and how it will be installed. There are many other threads on this site regarding geothermal that contain a lot of useful information. Have you considered in-floor hydronic radiant with geothermal? SR |
Here is a link that might be useful: International Ground Source Heat Pump Association
Thanks for the advice. In-floor radiant is ruled out due to additional costs (~$60-75,000 extra). Do you mean ClimateMaster's zoning would be better than Honeywell's setup? Should I ask both HVAC companies to quote for ClimateMaster's (Carrier's) zoning module? thanks |
I would encourage you to have a discussion about Carrier Infinity thermostats and zoning verses Honeywell. The discussion should also include the pros and cons of a mono block system (all in 1-package) verses a split system (separate geothermal and fan coil units). What I like about the Carrier Infinity thermostat with zoning is that it gives you the capacity to not only monitor humidity in A/C mode but to control the humidity as well. The set point in A/C mode can be either by temperature degree or by a relative humidity set point, which can save energy and enable adjustment for comfort without necessarily lowering the temperature, just reducing the humidity. Both systems are good, do some research before you meet with your contractors and draw your own informed conclusions. SR |
Here is a link that might be useful: Carrier Infinity Thermostat
SR, thank you so much for the advice. Will definitely ask the HVAC companies on the Infinity zoning. |
If you use the builder's HVAC company I imagine part of the price includes a profit for the builder. This is part of the differences in prices. Make sure you understand the limits of the federal tax credits for geothermal. Items like duct work are not eligible. |
I thought that the equipment associated with installation of a geothermal system does qualify for the federal tax credit. Here is the quote from Geothermal Genius website: I will also contact my tax accountant. |
Here is a link that explains what qualifies for the tax credit. You have to go to irs.gov about the explanation for the carry forward of energy tax credits. |
Here is a link that might be useful: What parts of a geothermal heat pump are covered by the tax credit?
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Rajahmundry: Pondicherry Lt Governor Dr
Iqbal Singh today said the government should make sure that
every citizen of the country gets clean water.
Singh, who was here to inaugurate Y S Rajasekhara Reddy
underground water pipeline at Dowleswaram, said the drinking
water must be preserved in view of changing global situation.
"If, we fail to preserve the drinking water, we would face
a severe water problem in 2020. Every citizen must preserve
water," Singh said. The government should implement new plans to provide clean drinking water to every citizen in the country, he said.
Pondicherry Revenue Minister Maladi Krishna said the
pipeline project is envisaged at at Rs 50 crore, out of which
Rs 32 crore would be provided by Central Government and
Pondicherry government will bear remaining Rs 18 crore.


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Best EXP Today: Judge World 32 +6,899,868 |
Worst EXP Today: Breks World 10 -14,149,205 |
First Hunting Tournament | |
As you might have already read in the news the first ever hunting tournament starts on TibiaME today Wednesday 17.12.14. After a short maintenance downtime you can participate and become the first ever world hunting champion on your world. As we are aware that many of you might have some questions about how the tournament works, we decided to give you a short summary on how the tournament system works: With the end of the downtime, the first hunting tournament in TibiaME will start. To participate you have to open the Marketplace and buy a tournament entrance ticket. ... See more» |
Category: TibiaME News Date: 2014-12-17
Upcoming downtime | |
Tomorrow Wednesday (17.12.2014) 09:00 CEST there will be a short downtime on all gameworlds for some maintenance work. After the downtime the first ever hunting tournament will start on each world to find the first world hunting champion. Come to Colossea and be part of it! |
Category: TibiaME News Date: 2014-12-16
Pet Experience | |
While doing some routine checks we discovered some balancing issues with the pets level up experience. The issues were rebalanced and will go live with the next server save. It is possible that some of your pets, especially the dragons, the pets of darkness or the Cerberus will rise in level. This is no bug but the result of the rebalancing. |
Category: TibiaME News Date: 2014-12-15
Erebos Information | |
Erebos Information With the next server save we will release a little fix for the NPC Jericho. You will then be able to get more information about the worldquest when talking to him. Besides the status of the barriers and the hell crystal delivery you can also ask him how much time is left until you need to have reached your goal. The time he gives is not exact but an approximation. Only in the last 30 minutes will he tell you exact amount of seconds you have left. |
Category: TibiaME News Date: 2014-12-11
Winterfeast Event 2014 | |
From December 22nd until January 11th the Winterfeast will be celebrated in TibiaME. Work together to earn a 10% EP Bonus for every monster killed and uncover the secret contents of the Winterfeast gifts! Article image(s): Image |
Category: TibiaME News Date: 2014-12-10
Stamina Calculator | |
Little stamina calculator been added: http://tibiame4all.com/?p=StaminaCalc As suggested: "You choose what time character was off and with what stamina.. then calculator say at what time stamina will be full" Image |
Category: Site News Date: 2014-12-08
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Deseo Hotel + Lounge
Stone steps echoing a Mayan temple lead to this minimalist-chic hotel's Christo-meets-pool-party, second-floor roof patio overlooking Fifth Avenue. At night, sheer white drapes wafting in the aqua-and-purple glow evoke an underwater dream. Join the stylish, predominantly 30ish crowd to lounge on large daybeds while watching wall-projected movies and imbibing the signature champagne-spiked mojito, a.k.a. the Cubano Royale. Service is leisurely, but the drinks are robust, especially the excellent martinis in flavors ranging from cucumber to chamoy, a Mexican candy.
Tune in on Wednesdays at 10PM ET for Top Chef: Boston, the 12th season of Bravo's Emmy-Award winning, hit reality series.


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Super Smash Bros. Videos, Questions, Answers, Hints, Walkthroughs, Cheats. (Nintendo 64)
Supercheats' unofficial guide for Super Smash Bros (3DS). The guide will discuss the game's mechanics, features, and will have in-depth analysis of each available fighter in the game.
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Check out our huge collection of cheats which include unlocking the Sound room, playing as Master Hand and doing 999% damage in seconds.
Play as ash or misty. And master hand.
We have 77 questions and 268 answers for this game. See if your question has already been asked or ask your own to get the exact game help you need for Super Smash Bros..
If you think you are great at Super Smash Bros. then test your knowledge by trying to answer the questions asked by our members, and help them out at the same time!
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Check out our videos to find out the danger of pipes and watch Captain Falcon kick Link of Hyrule Castle's edge.
We have 3 Walkthroughs for Super Smash Bros..
We have FAQ/Walkthroughs including a competitive strategy FAQ and a Target Test guide.
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The struggle committee which had been constituted to seek justice in the double murder case at Theroor last year has demanded a CBI probe into the death of forest department employee Arumugham and his wife Yogeswari.
According to sources, on November 21, 2011, Arumugham (50), a forest employee and his wife Yogeswari (42), were shot dead at Theroor near Suchindrum while they were returning home. Initially as there was no progress in the double murder case, the public from Theroor and the inland fishermen community, held various demonstrations demanding the police to expedite the investigation and to arrest the culprits involved.
In the meantime, on February 18, the district police arrested Sahayam, the district secretary of AIADMK Elaingar and Elangikal Peravai.
After this the police said that a total of 14 persons including Sahayam were involved in the case and arrested around three more. But till date, no one else who was allegedly involved in the case has been arrested. Moreover, as the police did not file a charge sheet against Sahayam even after 90 days after his arrest, he was freed on conditional bail.
The members of the struggle committee filed a public interest litigation in the Madurai Bench of the Madras High Court, demanding that the case be transferred to CBI for further investigation. When the petition came up for hearing, the Government pleader produced the status report of the investigation, stating that three persons had been arrested in this case and added that further investigation was in progress. After considering this, the court dismissed the petition and asked them to approach the appropriate forum if still aggrieved, said G. Selastin of the struggle committee.
He further said that the committee strongly suggested the involvement of some police personnel and officials from the forest department in the brutal murder of Arumugham and his wife.
Therefore the struggle committee has decided to submit a review petition demanding the transfer of the investigation to CBI, said Selastin.
Keywords: Theroor double murder case


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Let's organize a boycott of Obama's speech/propaganda on Tuesday
Submitted by amc1953 on Sun, 09/08/2013 - 10:18I hear Obama is going on the mind control box on Tuesday evening to try and convince Americans to strike Syria. What if everyone just refused to watch his pro war propaganda? What if they turned off the tv and called and e-mailed their congressmen instead and told them to vote no. Then they could spend the rest of the evening studying the constitution with their children, especially the part that says only the congress can declare war. Lets spread the word.
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Don't think this will take much organizing
There is already a renewed popularity for Board games and Card Games everytime he is on.
Unconstitutional War - "The story you are about to hear is true; the names and places are being changed to protect the guilty."
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Let's slam the door in
Let's slam the door in obama's face and watch Rand instead after obama is done telling his lies.
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His speech is going to be
His speech is going to be totally opposite of what everyone thinks it will. He's already backing down, and has probably already deceided that he isn't going to attack. With new developments of russia calling our bluff in asking syria to hand over its chem weapons, I think you're going to hear an entirely different tune than what you might expect.
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you could be right. Things
you could be right. Things have changed dramatically today. He knows he can't win a the vote in the house and I think the russian plan has given him a way to back off without losing too much face.
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Why would people not want to listen to a president's argument?
... especially for a possible war.
That seems a bit childish.
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it will be propaganda based
it will be propaganda based on lies. Better to just slam the door in his face.
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I would listen to a legitimate president.
Most of the RP people I know think this president is illegitimate.
I also try not to listen to Satan.
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Yes, the president is a Soviet dupe....
But... he's our Soviet dupe.
And we go to war with the president we have... not the president we might want or wish to have at a later time.
The geopolitical stakes are high on Syria... and just playing petty partisan politics doesn't do any good. We're stuck with Obama for another 3 long years... and the rapidly approaching post-western world needs a credible American president... even if he's only pretending.
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His speach is on 9/10?
Well.....Lets hope he doesn't announce a bunch of missing money or something.
God I hope nothing happens.
"If Tyranny and Oppression come to this land, it will be in the guise of fighting a foreign enemy." -- James Madison
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For a good reason why you should do this:
see my most recent post.
"Hence, naturally enough, my symbol for Hell is something like the bureaucracy of a police state or the office of a thoroughly nasty business concern." ~~C.S. Lewis
Love won! Deliverance from Tyranny is on the way! Col. 2:13-15
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I think its all part of a
I think its all part of a master plan for iran. First they weaken assad, then they have to send in the ground troops to secure the chemical weapons, then they have a staging area for the war on iran.
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In Communist Poland
In Poland during the final years of Communism, people would take a walk when the evening news was on as a form of protest. perhaps the same thing could be tried here.
"""One evening, just as the nightly television news came on, a Pole fed up with the daily dose of government propaganda got out of his chair, turned his TV set toward the window and went out for a stroll. No one in Swidnik, a factory town 100 miles southeast of Warsaw, claims to know just who made that first "news-walk," but within days almost the entire population of 30,000 began to crowd the tree-lined main street for an evening promenade during the 7:30 newscast. When local authorities clamped on a 7 o'clock curfew to counter the protest, the resourceful residents of Swidnik took a walk during the 5 o'clock news broadcast. Frustrated officials finally lifted the curfew, and after a month of newswalks, Swidnik's citizens decided they had made their point and stayed home—but not before their unique piece of resistance had spread to Olsztyn, Lublin, Bialystok, even Warsaw. Explained a Swidnik news-walker: "Every contact between the people and the authorities can be used to show dissatisfaction with martial law and everyone can do it in his own way."
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Thanks for the great comment.
Thanks for the great comment. The people of the USA have to break the stranglehold of the mind control box in order to reclaim their country.
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I agree
This is one instance where contacting our elected officials can actually make a difference. If they are flooded with responses showing that nearly 100% of their constituents are against military action in Syria it would make it difficult to actually vote for it. Hopefully the democrats will have enough guts to against the President.
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another great and quick way
another great and quick way is to sent a message via Facebook or Twitter or any other social media site that the politicians use to make a statement. I've seen a lot of hashtags take off and cause media attention.
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twitter, facebook, e-mail,
twitter, facebook, e-mail, call. Do everything you can think of to stop this war. If we can stop this war it will be the first time since tv was invented that the government's propaganda machine has not been able to manipulate the sheeple into supporting a war.
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I have been e-mailing my
I have been e-mailing my senator daily and ending with, it is your duty to represent the will of your constituents not follow the orders of the party leaders.
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WTTG Fox 5 spent the morning with the Clark School's TerpsRacing team on Wednesday, December 14, learning about the team and their Formula SAE and Baja vehicles. Reporter Annie Yu even took the Baja vehicle for a test drive!
Terps Racing Team: MyFoxDC.com
Clark School videos from the morning:
Driver/Clark School alum Mike Stanley in the Formula vehicle.
Annie Yu takes the Baja vehicle for a spin.
The Baja vehicle in action (watch for the curb jump!).
Video from a prior competition:
See what it's like to be in the driver's seat of the Formula car.
Related Articles: Clark School Baja Team Tops in U.S. Hovercraft Invade Kim Building Again NPR Features Gamera Team Clark School Team Unofficially Satisfies Two Sikorsky Prize Requirements Gamera Team Sets New Unofficial World Record for Human-Powered Helicopter Flight "Nebuchadnezzar" Takes Autonomous Hovercraft Honors Gamera Team Recognized by Helicopter Society Pepco Buys UMd.'s Solar Decathlon-Winning Home for Public Display Clark School's Gamera Certified for World Records Hoyer Honors UM Solar Decathlon Team
December 14, 2011
Current Headlines
Alumna Namrata Vaswani wins Best Paper Award
Alum Radha Poovendran named IEEE Fellow; department chair
Alumna Enlu Zhou wins NSF CAREER Award for optimization and sampling in stochastic simulation
UMD Online Engineering Programs Continue to Climb in Standings
Clark School Students Invited to Apply for Spring Engineering Course in Italy
Chellappa Elevated to AAAI Fellow
Naviasky, an ECE Student, Spoke at the 2014 Winter Commencement
Workshop on Geometry of Collective Behavior organized by P. S. Krishnaprasad
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Raj Singh's Mobile Life
At CTIA last week, I helped lead a session on "Making Money" at WIP JAM run by my friend, the mobile machine, Caroline Lewko. Anyways, a couple interesting notes came out of our discussion that I want to share – the question is whether I partner with an operator to distribute my application?
About Me
I'm Co-Founder/CEO of Tempo AI. You can email me at raj at rajansingh dot com or follow me @mobileraj
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Griffith Family Chiropractic's certified massage therapist designs a customized lineup of moves to melt away aches and tension. A one-hour session rewards your head's hard-working stilts with the massage modality of your choice, including Swedish relaxation, deep tissue, and therapeutic styles. Choose Swedish massage to knead subterranean stress and burrowing dust bunnies to the skin's surface so your therapist can briskly sweep them away. Those with chronic tension or pain can target troublesome areas in the crosshairs of the therapeutic option. Sports-worn sterns benefit from deep tissue attention, which offers a better way to hasten muscle recovery than lying on a bed of steaks or aggressively heckling debilitated quadriceps.
Scents of Serenity owner Melanie Mueller believes that putting your best face forward means using organic and natural skincare products. That's why her team of aestheticians employs the Éminence Organic Skin Care line, whose concoctions are free of artificial colors, synthetic perfumes, and harsh chemicals, such as parabens and Twinkie filling. Guests can wander to the spa's cozy lobby to purchase many of these elixirs, strolling across hardwood floors to a centerpiece shelf stocked with bottles, creams, and the emerald leaves of aloe plants.
Mueller's team is an educated bunch; all aestheticians are licensed by the Virginia Board of Cosmetology, and all massage therapists are certified through the Virginia Board of Nursing, as well as the National Certification Board for Therapeutic Massage and Bodywork. They dole out a variety of kneading techniques—from aromatherapy to hot stone—in treatment rooms canopied by cream-colored drapes and strings of luminescent orbs that melt into butter every time a patron says "ahhh."
At Brennan's Aesthetics, Dr. Teresa Stadler and her team help clients rejuvenate faces and fight the chemical changes that occur with age by administering FDA-approved Botox and Juvederm injections. Dr. Stadler knows that a good diet and exercise can only go so far in terms of keeping the body young, but she also understands that cosmetic surgery can be a big commitment. That is why she proffers minimally invasive injections to rid mugs of unsightly wrinkles, such as crow's feet and frown lines. Dr. Stadler also perks up visages with prescription Latisse treatments, which make eyelashes longer, darker, and fuller.
At Custom Complexions Skincare, no two facials are exactly alike because no two faces are exactly alike. Aestheticians customize each with Bioelements skincare products and essential oils suited to the client?s specific skin type. Some facials are even designed to treat common concerns?acne and aging or sensitive skin are frequent complaints?or uncommon ones, such as a tree frog stuck to your forehead. Plus, any treatment can be modified with microdermabrasion or chemical peels for enhanced skin-smoothing results.
Inside Sola Salon, stylist Nicole turns everyday strands into eye-catching displays of art. Bold highlights stand out against dark locks, braids keep hair in line, and voluminous curls nicely frame faces. Nicole also adds drama with hair extensions that can double as a cape on cooler days.


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Milan Fashion Week: Giorgio Armani Fall 2009
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Milan Fashion Week: Giorgio Armani Fall 2009
Milan 02/27/09. Photos by CHRISTOPHE SIMON/AFP/Getty Images & D. Venturelli/WireImage.
Read More 2009 Milan Fall Fashion Week2009 Milan Fashion Week2009 Fall Fashion Week2009 Fashion WeekGalleryRunwayGiorgio Armani
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- What will it take for Gap to make a comeback? — Style.com
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- Oops — you might be ruining your jewelry and not even know — Who What Wear
- Stock-photo models are people too! — Lucky
- Say goodbye to another brand: Jones New York is no longer — Fashionista
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Jon Jones shares his two cents regarding recent accusations against Bill Cosby.
The interim UFC Heavyweight Champion responds to Jon "Bones" Jones' recent comments.
Check out UFC's top fighters mean-mugging each other at today's big presser.
After losing Nike, "Bones" Jones close to deal with Reebok.
Cerrone-Jury moved from 1/18 in Boston to 1/3 in Las Vegas.
Daniel Cormier explains why he doesn't feel bad for Alexander Gustafsson.
DC confirmed as a new co-host of UFC Tonight on FOX Sports 1
Lorenzo Fertitta talkes about a number of exciting potential UFC Women's Bantamweight bouts on the horizon.
Heavyweight sluggers to duke it out on the Jones-Cormier undercard.
Dunham-Damm to serve as undercard bout for Jones-Cormier event in Las Vegas.
"Bones" Jones taunts "Mr. Wonderful" on Twitter.
D.C. also faces repercussions for his part in the brawl with "Bones" Jones.
"Bones" also ordered to complete 40 hours of community service.
Watch a heated stare down between Aldo and Mendes in Brazil.
Discussions are ongoing for a Machida vs. Rockhold fight at the end of the year.
Dana White is back from vacation and he's addressing several current UFC-related topics.
Alexander Gustafsson isn't pleased with the news that Cormier will still be Jones' next title challenger.
Danny Castillo vs. Tony Ferguson moved to co-main event position at UFC 177 on August 31st.
Jon Jones and Daniel Cormier react to the news of their fight being delayed.
Injury forces postponement of the Jon Jones vs. Daniel Cormier title fight.
Highlights and audio of a new interview with coach Mike Winklejohn.
After their joint-ESPN interview, things get even more heated between the UFC 178 headliners.
Early estimates for the UFC 175 pay-per-view are in.
The UFC color-commentator says what Jones and Cormier did is bad for the sport.
Check out a few clips from the champ's recent appearance on the popular late night talk show.


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VIDEO: Telematics boost, demand driving interoperable standard
Will the "Connected Vehicle" future have any benefit to independents? Exploring some of the possibilities here with Hirschbach Motor Lines' Jim Coffren's take from the fleet side.
Rand McNally unveils new IntelliRoute truck-specific GPS, mobile ELD compatibility
Rand McNally introduced Aug. 21 at the Great American Trucking Show new, updated versions of its line of truck-specific GPS units, the IntelliRoute TND. It also announced its HD 100 electronic logging device now works with smart phones, tablets and its IntelliRoute GPS devices.
TruckingOffice bookkeeping software introduces upgraded version with load planning and tracking functionality
Owner-operator/small-fleet software provider TruckingOffice announced a newly upgraded version of its online package, TruckingOffice Pro, designed to meet customer demand for more complex hauling,
VIDEO: Analysis of what's happening with ELDs / bankruptcies
Analyst Donald Broughton on what's happening behind a trend that is running against the usual for trucking bankruptcies: FMCSA's pressure on company over hours of service noncompliance.
Great American Trucking Show starts today: Here's how to follow the show
The Great American Trucking Show begins at noon Thursday,. Aug. 22. Here's how you can follow the show if you can't make it.
Diesel price hits 9-month low
The national average price for a gallon of on-highway diesel fell another eight-tenths of a cent in the week ended Aug. 18, putting it at $3.835. That's the lowest the national average price has been since the week ended Nov. 18 and the seventh straight week the average price has dropped.
Readers say no to speed governing, less equivocal on a national speed limit
Haven't we been here before? With some industry organizations calling for a national 65-mph speed limit and governing of Class 7 and 8 trucks to similar limits, readers weigh in.
10 things to see, do and know for the Great American Trucking Show
The Great American Trucking Show starts this week (Aug. 21) and has plenty for drivers and owner-operators. As a primer for the show, here are 10 things you can see or do or should know for the show.
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The YMCA Healthy Living Center™ (Y-HLC), located in Clive, Iowa, is on the leading edge of providing "medically integrated" programs in a unique healing environment.
The center will provide evidence-based programs for:
•Prevention | •Rehabilitation | •Restoration |
Start your warm up before you even check in! By parking further away from the Y you will leave more spaces available for our members with mobility difficulties and kick off your workout with a nice brisk walk at the same time.
~Thank you for your consideration~
NEW POST! Educate Motivate Participate
Sponsored by Above and Beyond Cancer
A blog that informs and inspires better health for living with more compassion and passion.
Click HERE for the latest blog entry.
Cycle for Neuro Wellness
What if you could reduce your Parkinson's symptoms, or those of someone you love, without the use of medications? And what if you could do it together with friends and loved ones?
Click HERE to read more about cycling for Neuro Wellness. This article in Live It! a publication of the Iowa Parkinson Disease Information and Referral Center written by Bill Roach, Execercise Instructor at the Y-HLC.
Weekly Education Lectures
Live Healthy, Be Healthy, Cook Healthy
LIVESTRONG at the YMCA
New schedules posted!
Click HERE to see all the schedules
*New Premium Fitness schedule available!
Return to Work after Disability Workshop
This one hour workshop will benefit any person looking for information on returning to work. This is a free workshop and open to the public. Monday, February 9th from 11am to 12pm. Click HERE for more info!
Athlete Development Clinic
Join us for an afternoon learning new training ideas, how to properly fuel your body and develop skills to help you reach your full athletic potential! This clinic will be from 1:00-4:30pm on Sunday, January 25th. Click HERE for more information and how to register.
New Winter Preschool Programs!
Would your child like to make friends, have fun, get active and discover who they are and what they can achieve? Join us for new winter activities here at the HLC. Programs start the week of January 5th! Click HERE for more information.
Better Choices, Better Health
How do you want to start your 2015? Would you like to breathe better? Overcome frustration? Reduce tiredness? Manage stress better? If you have a chronic condition such as diabetes, high blood pressure, arthritis, heart disease, etc...this program will teach you how to take an active role in managing your health. To learn more, click HERE.
Team Altitude - September News!
Check out the latest newsletter from Team Altitude! Click HERE for the newsletter.
Program for kids... Healthy Kids!
Healthy Kids families learn to make regular physical activity and healthy food choices part of a daily routine. Through interactive learning and activities for both parents and children together, Healthy Kids provides the foundation for healthy family homes. Program is $30 per child 7-12 years and comes with a free 12 week family membership to any of our YMCA branches. To learn more, click HERE
4 Weeks To A Healthier You - 4 Week program increasing self confidence to initiate and sustain positive healthy habits. New program starts every month!
Move Well Today - a 12 week diabetes exercise and education program.
To learn more about these programs - click HERE


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Costco, the chain of warehouse clubs that has become the nation's largest wine retailer, is suing its home state of Washington, claiming that its regulations on alcohol distribution violate fair trade law.
For the full article, check out the new issue of Wine Spectator, on newsstands May 15, 2004.
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News Details
Goodwood FOS to show Schumacher 1992 Benetton
The Benetton B191B driven by Michael Schumacher during the 1992 Formula One Season will be amongst the cars on display at this weekends Goodwood Festival of Speed in West Sussex.
Schumacher secured his maiden podium finish in this car at the 1992 Mexican Grand Prix and followed that up with a second podium at the Brazilian Grand Prix later that year.
The car was initially driven by Nelson Piquet in his final Grand Prix in Australia in 1991, and also by Martin Brundle in his debut for the team in South Africa 1992 before it switched over to Schumacher.
The car is now owned by Zak Brown, who is the founder and CEO of Just Marketing International (JMI), the world's largest motorsport...
Full Story »
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7mo ago from TheCheckeredFlag.co.uk
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The Green Bay Packers Hall of Fame Inc. Golf Classic is set for Monday, July 13, 2015, at The Bull in Sheboygan Falls, WI. Those interested in participating in the event can contact Holly Borga (920-965-6986; firstname.lastname@example.com) for more information.
The Green Bay Packers Hall of Fame Inc. will induct former Packers quarterback Brett Favre at the 45th Annual Hall of Fame Induction Banquet. The event will also feature the retirement of Favre's No. 4. This dual recognition is an honor that is unprecedented in Green Bay Packers history. The banquet will be held on Saturday, July 18, 2015, in the Lambeau Field Atrium.
All Packers pre-season and regular season games are carried live on television.
Select pre-season games will air statewide on local television. NBC 26 and WTMJ 4 will broadcast Packers preseason games that don't air nationally.
Game dates and times are posted on the Packers schedule page.
If your local station is not carrying the Packers game, be sure to check out Packers Everywhere, the place to find official Packers bars and events and connect with other fans to root for the Green & Gold. Every week, bars and restaurants near you will be hosting Packers fans to watch the game together. Come to Packerseverywhere.com to find these events, play fun games and enter contests for exclusive prizes.
Right now, Packers bars and restaurants are signing up across the country to become official Packers Everywhere bars. If you'd like to nominate a bar or restaurant, click here. If you'd work at a restaurant or bar and would like to become an official Packers Everywhere bar, click here.


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The new Archbishop Of Canterbury Justin Welby leaves St Paul�s... News Photo 160640775Adult,Caroline,Cathedral,Greater London,Horizontal,International Landmark,Justin Welby,Leaving,London - England,New,Post,Religion,St. Paul's Cathedral - London,UK,Wife,WomenPhotographer AFPCollection: AFP 2013 AFPThe new Archbishop Of Canterbury Justin Welby (L) leaves St Paul�s Cathedral with his wife Caroline after being confirmed into the post of Archbishop on February 4, 2013. The ceremony, known as the Confirmation of Election, forms part of the legal process by which Welby replaces his predecessor, Rowan Williams, as Archbishop of Canterbury. AFP PHOTO/Leon Neal (Photo credit should read LEON NEAL/AFP/Getty Images)


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I can't thank Joshua's enough for their extra effort to meet the deadline for our jewelry needs. We were "under the gun" to make our surprise...Read More ->
I was searching for rings all over the web and i came across Joshua Jewelry, after browsing the website, i fell in love with one of the sets, the fact...Read More ->
Writing to inform you that I am MORE than pleased with the workmanship and quality of my order. You even called me to keep me up-to-date and made sure my...Read More ->
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Air Jordan 1 High – "Melo"
Images have surfaced online heralding an Air Jordan 1 High that features classic color-blocking and a tongue tag branded with Carmelo Anthony's personal logo. The kicks are finished in an appealing two-tone theme highlighted by a neutral black base with dull gold accents, while a contrast white midsole … READ MORE
Air Jordan 1 Mid – Red Python and Sueded Croc by JBF Customs
While his Air Jordan 3 "BAPE x Ice Cream" Customs was making the rounds on sneaker blogs and kicks forums, JBF Customs was onto his next project – a pair of Air Jordan 1 Mid with genuine python and croc skins. A labor-intensify process where each selection, … READ MORE
Air Jordan 1 Mid – White/Cool Grey-White | Available Now
Wearily of all the "Triple Black" colorways recently? Jordan Brand is looking to add some variation into the mix in its new releases, including this Air Jordan 1 Mid in White/Cool Grey-White. Abbreviated as the Air Jordan 1 Mid "White/White" by some, the design is just a … READ MORE
Air Jordan 1 Mid – "Triple Black"
It seems like the "Triple Black" color scheme is making its round along Nike and Jordan Brand designs. After Air Max 95, Air Max 90, Hoodland Suede, and Flight 89, the colorway found its way to the Air Jordan 1 Mid. A mix of premium leather and … READ MORE
Air Jordan 1 Retro High OG – "NYC"
The follow up to the uber-limited edition Public School x Air Jordan 1 "PSNY", the Air Jordan 1 Retro High OG "NYC" releases today exclusively at the Jordan Brand Flight 23 store at Footaction. Much like the "PSNY", the "NYC" has raised lettering on both heels, as … READ MORE
Air Jordan 1 "Jeter" | Release Info
Considering the Bronx Bombers' less-than-stellar playoff chances, we could be rapidly approaching the end of the Derek Jeter era of the storied baseball franchise. Jordan Brand is sending off the Captain with his own edition of the Air Jordan 1, featuring the Yankees' signature navy and white and … READ MORE
fragment design x Air Jordan 1 Retro High OG | Preview
Hiroshi Fujiwara is evidently steering his fragment design brand into uncharted waters, readying an impending collaborative release focused on the venerable Air Jordan 1. As is normally the case with fragment design joint ventures, the aesthetic here is a classic one, showcasing the kind of color blocking … READ MORE
Air Jordan 1 KO High OG – "Sport Blue" | NikeStore Release Info
First launched in 1985, the Air Jordan 1 KO was originally made to serve as a complement to the original leather version. However, with Jordan Brand's rapid growth in popularity, so too has the Air Jordan 1 KO (aka AJKO). Now as a standalone product, the Air … READ MORE
Public School x Air Jordan 1 – "PSNY" | Detailed Look
After toiling for years in sweats and tears, Public School's designers Dao-Yi Chow and Maxwell Osborne are enjoying the fruits of their labor with a succession of awards. First came the 2013 CFDA/Vogue Fashion Fund, then the 2014 CFDA award for Menswear Designer of the Year followed … READ MORE
Public School x Air Jordan 1 – "PSNY/NY23"
As a gesture of thanks, Public School will be gifting a special Air Jordan 1 "PSNY" to the label's supporters. Referring to the attributes of New York City, home to Public School's designers Dao-Yi Chow and Maxwell Osborne, the Air Jordan 1 features premium leather upper in … READ MORE
Air Jordan 1 Retro High OG – "Gym Red"
Just a friendly reminder that the "Gym Red" Air Jordan 1 Retro High OG will be available this coming Saturday, June 28th. Vintage in appearance only, the "Gym Red" is actually a new colorway though with traces of the original "Banned" edition. A mix of red smooth … READ MORE


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The rooms here are small, but have all the usual amenities--mini frige, microwave, coffee maker and grinder(coffee beans are provided and you grind your own), TV, and free WiFi. What sets this place apart is the location( right outside of Sequoia NP) and the beautiful grounds. A patio is a step outside the door and it overlooks the river. Native plants abound. Birds and waterfowl are everywhere. It was pure joy to sit on the patio listening to water rush over rocks, smelling the scent of flowers in bloom, and watching hummingbirds, ducks, and even a blue heron. There are grills and a large swimming pool for guests use. A breakfast basket was provided at check in which contained fruit and pastries. I would return to Buckeye Tree over and over again.
- Official Description (provided by the hotel):
- Buckeye Tree Lodge is located 1/4 mile from the entrance to Sequoia National Park in the Sierra Nevada Mountains. With all rooms set along the banks of the Kaweah River, the river views and sounds from each room and balcony are spectacular. The Buckeye's beautiful grounds include a pool, lawn and picnic area next to the River. All rooms have private bathroom, cable TV, refrigerator, microwave, coffeemaker, AC and free local and long distance calling. ... more less
- Also Known As:
- Buckeye Tree Hotel Three Rivers
- Buckeye Tree Three Rivers


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"I have been making this recipe for years now my whloe family loves it. I shred myself too. Make this you won't regret it"
"Well this is delish I made this as written you don't need to change a thing. We had this BBQ there was none left my family says this is a keeper. The flavors blend perfectly. I don't know what you changed from the original but you did a super job!!! This will be made agian and again."
"I printed this on 03 I guess I should review itLOL. It is great I make it as written. having it today with grille ribs."
"I should review this 3 times that's how many times I have made it so far. Had friends who had been ill. Took this to them they loved it also. Mine at home I served with homemade noodles."
"I can't believe I haven't reveiwd this yet. I have made this several times. I always make as written. I always serve with rice and a veggie. Very easy, VERY GOOD!!!"
"This was out of this world!! We ate it all . I had to make a couple of changes which I don't normally do the first time I make a new dish. It was only that I used a can of diced tom drained, no green pepper,and 2 cups sour crem. served over homemade noodles. PLEASE TRY THIS!!!"
"This was great made as written would not change a thing"
"I don't know why I haven't reviewed this yet I think I printed this in 2005 and have been making it ever since just as written it is delish!!!"


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Publications (45)286.07 Total impact
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ABSTRACT: The C57BL/6:Slc23a13(-/-);Gpd2(-/-) double-knockout (a.k.a., citrin/mitochondrial glycerol 3-phosphate dehydrogenase double knockout or Ctrn/mGPD-KO) mouse displays phenotypic attributes of both neonatal intrahepatic cholestasis (NICCD) and adult-onset type II citrullinemia (CTLN2), making it a suitable model of human citrin deficiency. In the present study, we show that when mature Ctrn/mGPD-KO mice are switched from a standard chow diet (CE-2) to a purified maintenance diet (AIN-93M), this resulted in a significant loss of body weight as a result of reduced food intake compared to littermate mGPD-KO mice. However, supplementation of the purified maintenance diet with additional protein (from 14% to 22%; and concomitant reduction or corn starch), or with specific supplementation with alanine, sodium glutamate, sodium pyruvate or medium-chain triglycerides (MCT), led to increased food intake and body weight gain near or back to that on chow diet. No such effect was observed when supplementing the diet with other sources of fat that contain long-chain fatty acids. Furthermore, when these supplements were added to a sucrose solution administered enterally to the mice, which has been shown previously to lead to elevated blood ammonia as well as altered hepatic metabolite levels in Ctrn/mGPP-KO mice, this led to metabolic correction. The elevated hepatic glycerol 3-phosphate and citrulline levels after sucrose administration were suppressed by the administration of sodium pyruvate, alanine, sodium glutamate and MCT, although the effect of MCT was relatively small. Low hepatic citrate and increased lysine levels were only found to be corrected by sodium pyruvate, while alanine and sodium glutamate both corrected hepatic glutamate and aspartate levels. Overall, these results suggest that dietary factors including increased protein content, supplementation of specific amino acids like alanine and sodium glutamate, as well as sodium pyruvate and MCT all show beneficial effects on citrin deficiency by increasing the carbohydrate tolerance of Ctrn/mGPD-KO mice, as observed through increased food intake and maintenance of body weight.Molecular Genetics and Metabolism 08/2012; · 2.83 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The citrin/mitochondrial glycerol-3-phosphate dehydrogenase (mGPD) double-knockout mouse displays phenotypic attributes of both neonatal intrahepatic cholestasis and adult-onset type II citrullinemia, making it a suitable model of human citrin deficiency. In the present study, we investigated metabolic disturbances in the livers of wild-type, citrin (Ctrn) knockout, mGPD knockout, and Ctrn/mGPD double-knockout mice following oral sucrose versus saline administration using metabolomic approaches. By using gas chromatography/mass spectrometry and capillary electrophoresis/mass spectrometry, we found three general groupings of metabolite changes in the livers of the double-knockout mice following sucrose administration that were subsequently confirmed using liquid chromatography/mass spectrometry or enzymatic methods: a marked increase of hepatic glycerol 3-phosphate, a generalized decrease of hepatic tricarboxylic acid cycle intermediates, and alterations of hepatic amino acid levels related to the urea cycle or lysine catabolism including marked increases in citrulline and lysine. Furthermore, concurrent oral administration of sodium pyruvate with sucrose ameliorated the hyperammonemia induced by sucrose, as had been shown previously, as well as almost completely normalizing the hepatic metabolite perturbations found. Overall, we have identified additional metabolic disturbances in double-KO mice following oral sucrose administration, and provided further evidence for the therapeutic use of sodium pyruvate in our mouse model of citrin deficiency.Molecular Genetics and Metabolism 08/2011; 104(4):492-500. · 2.83 Impact Factor - Endocrine Journal 01/2010; 57(2):141-152. · 2.02 Impact Factor
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ABSTRACT: Adenosine 5' -monophosphate-activated protein kinase (AMPK) has been implicated in the regulation of energy metabolism, although its role in the pancreatic beta cells remains unclear. In the present, we have overexpressed a dominant negative form of AMPKalpha1 subunit (Asp57Ala) tagged with c-myc epitope (AMPKalpha1-DN) in INS-1D cells with an adenoviral vector. After 48 h of adenoviral infection, overexpression of AMPKalpha1-DN in INS-1D cells was confirmed by Western blot analysis with anti-c-myc antibody. Phosphorylation of the Thr172 in AMPKalpha1/alpha2 subunit was progressively decreased in parallel with increasing number of adenoviral titers. Glucose-stimulated insulin secretion in response to 30 mmol/L glucose was decreased in INS-1D cells overexpressing AMPKalpha1-DN as compared to control cells infected with adeno- LacZ vector. Neither cellular insulin content nor insulin mRNA level was changed between the two groups. Phosphorylation of acetyl-CoA carboxylase (ACC), a down-stream substrate of AMPK, was decreased, indicating that ACC activity was increased, due to the decreased AMPK activity. In fact, intracellular triglyceride content was increased as compared to control cells. The beta-oxidation of palmitate was decreased at 30 mmol/L glucose. Insulin secretion in response to potassium chloride or glibenclamide was also decreased as compared to control cells. In conclusion, suppression of AMPK activity in beta-cells inhibited insulin secretion in response to glucose, potassium chloride or glibenclamide without altering insulin content. Accumulation of triglyceride subsequent to the activation of ACC by suppression of AMPK activity, was suggested to be, at least in part, responsible for the impaired insulin secretion through so-called lipotoxicity mechanism.Endocrine Journal 11/2009; 57(2):141-52. · 2.02 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A decrease in plasma adiponectin levels has been shown to contribute to the development of diabetes. However, it remains uncertain whether adiponectin plays a role in the regulation of insulin secretion. In this study, we investigated whether adiponectin may be involved in the regulation of insulin secretion in vivo and in vitro. The effect of adiponectin on insulin secretion was measured in vitro and in vivo, along with the effects of adiponectin on ATP generation, membrane potentials, Ca2+ currents, cytosolic calcium concentration and state of 5'-AMP-activated protein kinase (AMPK). In addition, insulin granule transport was measured by membrane capacitance and total internal reflection fluorescence (TIRF) analysis. Adiponectin significantly stimulated insulin secretion from pancreatic islets to approximately 2.3-fold the baseline value in the presence of a glucose concentration of 5.6 mmol/l. Although adiponectin had no effect on ATP generation, membrane potentials, Ca2+ currents, cytosolic calcium concentrations or activation status of AMPK, it caused a significant increase of membrane capacitance to approximately 2.3-fold the baseline value. TIRF analysis revealed that adiponectin induced a significant increase in the number of fusion events in mouse pancreatic beta cells under 5.6 mmol/l glucose loading, without affecting the status of previously docked granules. Moreover, intravenous injection of adiponectin significantly increased insulin secretion to approximately 1.6-fold of baseline in C57BL/6 mice. The above results indicate that adiponectin induces insulin secretion in vitro and in vivo.Diabetologia 06/2008; 51(5):827-35. · 6.88 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Citrin is the liver-type mitochondrial aspartate-glutamate carrier that participates in urea, protein, and nucleotide biosynthetic pathways by supplying aspartate from mitochondria to the cytosol. Citrin also plays a role in transporting cytosolic NADH reducing equivalents into mitochondria as a component of the malate-aspartate shuttle. In humans, loss-of-function mutations in the SLC25A13 gene encoding citrin cause both adult-onset type II citrullinemia and neonatal intrahepatic cholestasis, collectively referred to as human citrin deficiency. Citrin knock-out mice fail to display features of human citrin deficiency. Based on the hypothesis that an enhanced glycerol phosphate shuttle activity may be compensating for the loss of citrin function in the mouse, we have generated mice with a combined disruption of the genes for citrin and mitochondrial glycerol 3-phosphate dehydrogenase. The resulting double knock-out mice demonstrated citrullinemia, hyperammonemia that was further elevated by oral sucrose administration, hypoglycemia, and a fatty liver, all features of human citrin deficiency. An increased hepatic lactate/pyruvate ratio in the double knock-out mice compared with controls was also further elevated by the oral sucrose administration, suggesting that an altered cytosolic NADH/NAD(+) ratio is closely associated with the hyperammonemia observed. Microarray analyses identified over 100 genes that were differentially expressed in the double knock-out mice compared with wild-type controls, revealing genes potentially involved in compensatory or downstream effects of the combined mutations. Together, our data indicate that the more severe phenotype present in the citrin/mitochondrial glycerol-3-phosphate dehydrogenase double knock-out mice represents a more accurate model of human citrin deficiency than citrin knock-out mice.Journal of Biological Chemistry 09/2007; 282(34):25041-52. · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The homeodomain transcription factor pancreas duodenum homeobox-1 (PDX-1) is a key regulator of pancreatic beta-cell development, function, and survival. Deficits in PDX-1 expression result in insulin deficiency and hyperglycemia. We previously found that the glucose-responsive transcription factor early growth response-1 (Egr-1) activates the insulin promoter in part by increasing expression levels of PDX-1. We now report that Egr-1 binds and activates multiple regulatory sites within the pdx-1 promoter. We identified consensus Egr-1 recognition sequences within proximal and distal regions of the mouse pdx-1 promoter and demonstrated specific binding of Egr-1 by chromatin immunoprecipitation and electrophoretic mobility shift assays. Overexpression of Egr-1 increased transcriptional activation of the -4500 proximal pdx-1 promoter and of the highly conserved regulatory Areas I, II, and III. Mutagenesis of a specific Egr-1 binding site within Area III substantially decreased Egr-1-mediated activation. Egr-1 increased the transcriptional activation of Areas I and II, despite the absence of Egr-1 recognition sequences within this promoter segment, suggesting that Egr-1 also can regulate the pdx-1 promoter indirectly. Egr-1 increased, and a dominant-negative Egr-1 mutant repressed, the transcriptional activation of distal pdx-1 promoter sequences. Mutagenesis of a specific Egr-1 binding site within regulatory Area IV reduced basal and Egr-1-mediated transcriptional activation. Our data indicate that Egr-1 regulates expression of PDX-1 in pancreatic beta-cells by both direct and indirect activation of the pdx-1 promoter. We propose that Egr-1 expression levels may act as a sensor in pancreatic beta-cells to translate extracellular signals into changes in PDX-1 expression levels and pancreatic beta-cell function.Journal of Biological Chemistry 04/2007; 282(9):5973-83. · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Glucokinase (Gck) functions as a glucose sensor for insulin secretion, and in mice fed standard chow, haploinsufficiency of beta cell-specific Gck (Gck(+/-)) causes impaired insulin secretion to glucose, although the animals have a normal beta cell mass. When fed a high-fat (HF) diet, wild-type mice showed marked beta cell hyperplasia, whereas Gck(+/-) mice demonstrated decreased beta cell replication and insufficient beta cell hyperplasia despite showing a similar degree of insulin resistance. DNA chip analysis revealed decreased insulin receptor substrate 2 (Irs2) expression in HF diet-fed Gck(+/-) mouse islets compared with wild-type islets. Western blot analyses confirmed upregulated Irs2 expression in the islets of HF diet-fed wild-type mice compared with those fed standard chow and reduced expression in HF diet-fed Gck(+/-) mice compared with those of HF diet-fed wild-type mice. HF diet-fed Irs2(+/-) mice failed to show a sufficient increase in beta cell mass, and overexpression of Irs2 in beta cells of HF diet-fed Gck(+/-) mice partially prevented diabetes by increasing beta cell mass. These results suggest that Gck and Irs2 are critical requirements for beta cell hyperplasia to occur in response to HF diet-induced insulin resistance.Journal of Clinical Investigation 02/2007; 117(1):246-57. · 13.77 Impact Factor -
Article: Regulation of insulin gene transcription by the immediate-early growth response gene Egr-1.
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ABSTRACT: Changes in extracellular glucose levels regulate the expression of the immediate-early response gene and zinc finger transcription factor early growth response-1 (Egr-1) in insulin-producing pancreatic beta-cells, but key target genes of Egr-1 in the endocrine pancreas have not been identified. We found that overexpression of Egr-1 in clonal (INS-1) beta-cells increased transcriptional activation of the rat insulin I promoter. In contrast, reductions in Egr-1 expression levels or function with the introduction of either small interfering RNA targeted to Egr-1 (siEgr-1) or a dominant-negative form of Egr-1 decreased insulin promoter activation, and siEgr-1 suppressed insulin gene expression. Egr-1 did not directly interact with insulin promoter sequences, and mutagenesis of a potential G box recognition sequence for Egr-1 did not impair the Egr-1 responsiveness of the insulin promoter, suggesting that regulation of insulin gene expression by Egr-1 is probably mediated through additional transcription factors. Overexpression of Egr-1 increased, and reduction of Egr-1 expression decreased, transcriptional activation of the glucose-responsive FarFlat minienhancer within the rat insulin I promoter despite the absence of demonstrable Egr-1-binding activity to FarFlat sequences. Notably, augmenting Egr-1 expression levels in insulin-producing cells increased the mRNA and protein expression levels of pancreas duodenum homeobox-1 (PDX-1), a major transcriptional regulator of glucose-responsive activation of the insulin gene. Increasing Egr-1 expression levels enhanced PDX-1 binding to insulin promoter sequences, whereas mutagenesis of PDX-1-binding sites reduced the capacity of Egr-1 to activate the insulin promoter. We propose that changes in Egr-1 expression levels in response to extracellular signals, including glucose, can regulate PDX-1 expression and insulin production in pancreatic beta-cells.Endocrinology 07/2006; 147(6):2923-35. · 4.64 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Multiple forms of heritable diabetes are associated with mutations in transcription factors that regulate insulin gene transcription and the development and maintenance of pancreatic beta-cell mass. The coactivator Bridge-1 (PSMD9) regulates the transcriptional activation of glucose-responsive enhancers in the insulin gene in a dose-dependent manner via PDZ domain-mediated interactions with E2A transcription factors. Here we report that the pancreatic overexpression of Bridge-1 in transgenic mice reduces insulin gene expression and results in insulin deficiency and severe diabetes. Dysregulation of Bridge-1 signaling increases pancreatic apoptosis with a reduction in the number of insulin-expressing pancreatic beta-cells and an expansion of the complement of glucagon-expressing pancreatic alpha-cells in pancreatic islets. Increased expression of Bridge-1 alters pancreatic islet, acinar, and ductal architecture and disrupts the boundaries between endocrine and exocrine cellular compartments in young adult but not neonatal mice, suggesting that signals transduced through this coactivator may influence postnatal pancreatic islet morphogenesis. Signals mediated through the coactivator Bridge-1 may regulate both glucose homeostasis and pancreatic beta-cell survival. We propose that coactivator dysfunction in pancreatic beta-cells can limit insulin production and contribute to the pathogenesis of diabetes.Molecular Endocrinology 02/2006; 20(1):167-82. · 4.20 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Heterozygous peroxisome proliferator-activated receptor-gamma (PPAR-gamma)-deficient (PPARgamma(+/-)) mice were protected from high-fat diet-induced insulin resistance. To determine the impact of systemic reduction of PPAR-gamma activity on beta-cell function, we investigated insulin secretion in PPARgamma(+/-) mice on a high-fat diet. Glucose-induced insulin secretion in PPARgamma(+/-) mice was impaired in vitro. The tissue triglyceride (TG) content of the white adipose tissue, skeletal muscle, and liver was decreased in PPARgamma(+/-) mice, but it was unexpectedly increased in the islets, and the increased TG content in the islets was associated with decreased glucose oxidation. Administration of a PPAR-gamma agonist, pioglitazone, reduced the islet TG content in PPARgamma(+/-) mice on a high-fat diet and ameliorated the impaired insulin secretion in vitro. Our results demonstrate that PPAR-gamma protects islets from lipotoxicity by regulating TG partitioning among tissues and that a PPAR-gamma agonist can restore impaired insulin secretion under conditions of islet fat accumulation.Diabetes 12/2004; 53(11):2844-54. · 8.47 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Triglyceride (TG) accumulation in pancreatic beta-cells is thought to be associated with impaired insulin secretory response to glucose (lipotoxicity). To better understand the mechanism of the impaired insulin secretory response to glucose in beta-cell lipotoxicity, we overexpressed a constitutively active form of the sterol regulatory element-binding protein- 1c (SREBP-1c), a master transcriptional factor of lipogenesis, in INS-1 cells with an adenoviral vector. This treatment was associated with strong activation of transcription of the genes involved in fatty acid biosynthesis, increased cellular TG content, severely blunted glucose-stimulated insulin secretion, and enhanced expression of the uncoupling protein-2 (UCP-2), which supposedly dissipates the mitochondrial electrochemical potential. To decrease the up-regulated UCP-2 expression, small interfering RNA for UCP-2 was used. Introduction of the small interfering RNA increased the ATP/ADP ratio and partially rescued the glucose-stimulated insulin secretion in the cells overexpressing SREBP-1c, but did not affect the cellular TG content. Next, the effect of the AMP-activated protein kinase (AMPK) agonist, 5-amino-4-imidazolecarboxamide riboside, was examined in the lipotoxicity model. Exposure of the cells with lipotoxicity to 5-amino-4-imidazolecarboxamide riboside increased free fatty acid oxidation, partially reversed the TG accumulation, phosphorylated AMPK and acetyl-coenzyme A carboxylase, and improved the impaired glucose-stimulated insulin secretion. These results suggest that UCP-2 down-regulation and AMPK activation could be candidate targets for releasing beta-cells from lipotoxicity.Endocrinology 09/2004; 145(8):3566-77. · 4.64 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: A role for mitochondrial glycerol-3-phosphate dehydrogenase (mGPD) in thermogenesis was investigated in transgenic mice lacking the mGPD gene (mGPD-/-). Reared and studied at 22 C, these mice have a small, but significant, reduction (7-10%) in energy expenditure, as evidenced by oxygen consumption (QO2) and food intake, and show signs of increased brown adipose tissue (BAT) stimulation, higher plasma T4 and T3 concentrations, as well as increased uncoupling protein 3 (UCP3) expression in muscle. When acclimated at thermoneutrality temperature (32 C), QO2 decreased in both genotypes, but the difference between them widened to 16%, whereas BAT underwent atrophy, and plasma T4 and T3 levels and UCP3 mRNA decreased, yet T3 and UCP3 persisted at significantly higher levels in mGPD-/- mice. Such differences disappeared when the mice were rendered hypothyroid. A compensatory role for the observed changes in BAT, thyroid hormone levels, and UCP3 was investigated with a 2-h cold challenge of 12 C in euthyroid and hypothyroid mice. No hypothermia ensued if the mice had been acclimated at 22 C, but when acclimated at 32 C, euthyroid mGPD-/- mice became significantly more hypothermic than the wild-type controls. When rendered hypothyroid, this difference was accentuated, and the mGPD-/- mice developed profound hypothermia ( approximately 28 vs. 34 C in wild-type mice; P < 0.001). Thus, mGPD-deficient mice have, despite increased plasma T4 and T3, a small, but distinct, reduction in obligatory thermogenesis, which is compensated by increased BAT facultative thermogenesis and by thyroid hormone-dependent mechanisms using other proteins, possibly UCP3. The results support a role for mGPD in thyroid hormone thermogenesis.Endocrinology 01/2004; 144(12):5469-79. · 4.64 Impact Factor - 01/2004;
Article: Pdx1 expression in Irs2-deficient mouse beta-cells is regulated in a strain-dependent manner.
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ABSTRACT: We previously demonstrated that Irs2-/- mice develop diabetes due to beta-cell growth failure and insulin resistance; however, glucose-induced insulin secretion was increased in islets isolated from Irs2-/- mice. Pdx-1, a transcription factor important for maintenance of the beta-cell function, was recently reported to be severely reduced in Irs2-/- murine beta-cells. We report herein that Pdx-1 expression, including the amount of Pdx-1 localized in the nucleus, is not down-regulated in our Irs2-/- murine beta-cells with a C57BL/6 background. We have also demonstrated the expression of upstream genes of Pdx-1, such as HNF3beta and HNF1alpha, as well as its downstream genes, including insulin, Glut2, and Nkx6.1, to be well preserved. We have further demonstrated Pdx-1 expression to also be preserved in beta-cells of 30-week-old diabetic Irs2-/- mice. In addition, surprisingly, even in Irs2-/- mice on a high fat diet with markedly elevated blood glucose, exceeding 400 mg/dl, Pdx-1 expression was not reduced. Furthermore, we found Pdx-1 to be markedly decreased in certain severely diabetic Irs2-/- mice with a mixed C57BL/6J x 129Sv background. We conclude that 1) Pdx-1 expression in Irs2-/- mice is regulated in a strain-dependent manner, 2) Irs2-/- mice develop diabetes associated with beta-cell growth failure even when Pdx1 expression is preserved, and 3) Pdx-1 expression is preserved in severely hyperglycemic Irs2-/- mice with a C57BL/6 background on a high fat diet.Journal of Biological Chemistry 11/2003; 278(44):43691-8. · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: We studied acute changes of secretory vesicle pH in pancreatic beta-cells with a fluorescent pH indicator, lysosensor green DND-189. Fluorescence was decreased by 0.66 +/- 0.10% at 149 +/- 16 s with 22.2 mM glucose stimulation, indicating that vesicular pH was alkalinized by approximately 0.016 unit. Glucose-responsive pH increase was observed when cytosolic Ca2+ influx was blocked but disappeared when an inhibitor of glycolysis or mitochondrial ATP synthase was present. Glutamate dimethyl ester (GME), a plasma membrane-permeable analog of glutamate, potentiated glucose-stimulated insulin secretion at 5 mM without changing cellular ATP content or cytosolic Ca2+ concentration ([Ca2+]). Application of GME at basal glucose concentration decreased DND-189 fluorescence by 0.83 +/- 0.19% at 38 +/- 2 s. These results indicated that the acutely alkalinizing effect of glucose on beta-cell secretory vesicle pH was dependent on glucose metabolism but independent of modulations of cytosolic [Ca2+]. Moreover, glutamate derived from glucose may be one of the mediators of this alkalinizing effect of glucose, which may have potential relevance to the alteration of secretory function by glutamate.AJP Endocrinology and Metabolism 09/2003; 285(2):E262-71. · 4.09 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Although we and others have generated IRS-2 knock-out (IRS-2(-/-)) mice, significant differences were seen between the two lines of IRS-2(-/-) mice in the severity of diabetes and alterations of beta-cell mass. It has been reported that although IRS-1 and IRS-3 knock-out mice showed normal blood glucose levels, IRS-1/IRS-3 double knock-out mice exhibited marked hyperglycemia. Thus, IRS-1 and IRS-3 compensate each other's functions in maintaining glucose homeostasis. To assess the effect of genetic background and also ablation of IRS-3 on IRS-2(-/-), we generated IRS-2/IRS-3 double knock-out (IRS-2(-/-)IRS-3(-/-)) mice by crossing IRS-3(-/-) mice (129/Sv and C57Bl/6 background) with our IRS-2(-/-) mice (CBA and C57Bl/6 background). Intercrosses of IRS-2(+/-)IRS-3(+/-) mice yielded nine genotypes, and all of them including IRS-2(-/-)IRS-3(-/-) mice were apparently healthy and showed normal growth. However, at 10-20 weeks of age, 20-30% mice carrying a null mutation for the IRS-2 gene, irrespective of the IRS-3 genotype, developed diabetes. When mice with diabetes were excluded from the analysis of glucose and insulin tolerance test, IRS-2(-/-)IRS-3(-/-) showed a degree of glucose intolerance and insulin resistance similar to those of IRS-2(-/-) mice. Both IRS-2(-/-) and IRS-2(-/-)IRS-3(-/-) mice had moderately reduced beta-cell mass despite having insulin resistance. Insulin-positive beta-cells were decreased to nearly zero in IRS-2(-/-) mice with diabetes. Although Pdx1 and glucose transporter 2 expressions were essentially unaltered in islets from IRS-2(-/-) mice without diabetes, they were dramatically decreased in IRS-2(-/-) mice with diabetes. Taken together, these observations indicate that IRS-3 does not play a role compensating for the loss of IRS-2 in maintaining glucose homeostasis and that the severity of diabetes in IRS-2(-/-) mice depends upon genetic background, suggesting the existence of modifier gene(s) for diabetes in mice of the 129/Sv genetic strain.Journal of Biological Chemistry 05/2003; 278(16):14284-90. · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The adipocyte-derived hormone adiponectin has been shown to play important roles in the regulation of energy homeostasis and insulin sensitivity. In this study, we analyzed globular domain adiponectin (gAd) transgenic (Tg) mice crossed with leptin-deficient ob/ob or apoE-deficient mice. Interestingly, despite an unexpected similar body weight, gAd Tg ob/ob mice showed amelioration of insulin resistance and beta-cell degranulation as well as diabetes, indicating that globular adiponectin and leptin appeared to have both distinct and overlapping functions. Amelioration of diabetes and insulin resistance was associated with increased expression of molecules involved in fatty acid oxidation such as acyl-CoA oxidase, and molecules involved in energy dissipation such as uncoupling proteins 2 and 3 and increased fatty acid oxidation in skeletal muscle of gAd Tg ob/ob mice. Moreover, despite similar plasma glucose and lipid levels on an apoE-deficient background, gAd Tg apoE-deficient mice showed amelioration of atherosclerosis, which was associated with decreased expression of class A scavenger receptor and tumor necrosis factor alpha. This is the first demonstration that globular adiponectin can protect against atherosclerosis in vivo. In conclusion, replenishment of globular adiponectin may provide a novel treatment modality for both type 2 diabetes and atherosclerosis.Journal of Biological Chemistry 02/2003; 278(4):2461-8. · 4.60 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Triacylglyceride (TG) accumulation in pancreatic beta-cells is associated with impaired insulin secretion, which is called lipotoxicity. To gain a better understanding of the pathophysiology of lipotoxicity, we generated three models of dysregulated fatty acid metabolism in beta-cells. The overexpression of sterol regulatory element binding protein-1c induced lipogenic genes and TG accumulation. Under these conditions, we observed a decrease in glucose oxidation and upregulation of uncoupling protein-2, which might be causally related to the decreased glucose-stimulated insulin secretion. The overexpression of AMP-activated protein kinase was accompanied by decreased lipogenesis, increased fatty acid oxidation, and decreased glucose oxidation; insulin secretions to glucose and depolarization stimuli were decreased, probably because of the decrease in glucose oxidation and cellular insulin content. It was notable that the secretory response to palmitate was blunted, which would suggest a role of the fatty acid synthesis pathway, but not its oxidative pathway in palmitate-stimulated insulin secretion. Finally, we studied islets of PPAR-gamma(+/-) mice that had increased insulin sensitivity and low TG content in white adipose tissue, skeletal muscle, and liver. On a high-fat diet, glucose-stimulated insulin secretion was decreased in association with increased TG content in the islets, which might be mediated through the elevated serum free fatty acid levels and their passive transport into beta-cells. These results revealed some aspects about the mechanisms by which alterations of fatty acid metabolism affect beta-cell functions.Diabetes 01/2003; 51 Suppl 3:S414-20. · 8.47 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Adiponectin (Ad) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. However, the signaling pathways that mediate the metabolic effects of Ad remain poorly identified. Here we show that phosphorylation and activation of the 5'-AMP-activated protein kinase (AMPK) are stimulated with globular and full-length Ad in skeletal muscle and only with full-length Ad in the liver. In parallel with its activation of AMPK, Ad stimulates phosphorylation of acetyl coenzyme A carboxylase (ACC), fatty-acid oxidation, glucose uptake and lactate production in myocytes, phosphorylation of ACC and reduction of molecules involved in gluconeogenesis in the liver, and reduction of glucose levels in vivo. Blocking AMPK activation by dominant-negative mutant inhibits each of these effects, indicating that stimulation of glucose utilization and fatty-acid oxidation by Ad occurs through activation of AMPK. Our data may provide a novel paradigm that an adipocyte-derived antidiabetic hormone, Ad, activates AMPK, thereby directly regulating glucose metabolism and insulin sensitivity in vitro and in vivo.Nature Medicine 12/2002; 8(11):1288-95. · 28.05 Impact Factor
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Teikyo University
- Department of Medicine
Edo, Tōkyō, Japan
The University of Tokyo
- • Faculty & Graduate School of Medicine
- • Department of Diabetes and Metabolic Diseases
- • Department of Internal Medicine
- • Division of Internal Medicine
Tokyo, Tokyo-to, Japan
Harvard Medical School
Boston, Massachusetts, United States -
Massachusetts General Hospital
- Laboratory of Molecular Endocrinology
Boston, MA, United States