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Publications (45)107.31 Total impact
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ABSTRACT: The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.Bioscience Biotechnology and Biochemistry 05/2012; 76(5):1051-4. · 1.27 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The degQ gene of Bacillus subtilis (natto), encoding a small peptide of 46 amino acids, is essential for the synthesis of extracellular poly-gamma-glutamate (γPGA). To elucidate the role of DegQ in γPGA synthesis, we knocked out the degQ gene in Bacillus subtilis (natto) and screened for suppressor mutations that restored γPGA synthesis in the absence of DegQ. Suppressor mutations were found in degS, the receptor kinase gene of the DegS-DegU two-component system. Recombinant DegS-His(6) mutant proteins were expressed in Escherichia coli cells and subjected to an in vitro phosphorylation assay. Compared with the wild type, mutant DegS-His(6) proteins showed higher levels of autophosphorylation (R208Q, M195I, L248F, and D250N), reduced autodephosphorylation (D250N), reduced phosphatase activity toward DegU, or a reduced ability to stimulate the autodephosphorylation activity of DegU (R208Q, D249G, M195I, L248F, and D250N) and stabilized DegU in the phosphorylated form. These mutant DegS proteins mimic the effect of DegQ on wild-type DegSU in vitro. Interestingly, DegQ stabilizes phosphorylated DegS only in the presence of DegU, indicating a complex interaction of these three proteins.Applied and Environmental Microbiology 09/2011; 77(23):8249-58. · 3.95 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The peptidoglycan of Selenomonas ruminantium is covalently bound to cadaverine (PG-cadaverine), which likely plays a significant role in maintaining the integrity of the cell surface structure. The outer membrane of this bacterium contains a 45-kDa major protein (Mep45) that is a putative peptidoglycan-associated protein. In this report, we determined the nucleotide sequence of the mep45 gene and investigated the relationship between PG-cadaverine, Mep45, and the cell surface structure. Amino acid sequence analysis showed that Mep45 is comprised of an N-terminal S-layer-homologous (SLH) domain followed by α-helical coiled-coil region and a C-terminal β-strand-rich region. The N-terminal SLH domain was found to be protruding into the periplasmic space and was responsible for binding to peptidoglycan. It was determined that Mep45 binds to the peptidoglycan in a manner dependent on the presence of PG-cadaverine. Electron microscopy revealed that defective PG-cadaverine decreased the structural interactions between peptidoglycan and the outer membrane, consistent with the proposed role for PG-cadaverine. The C-terminal β-strand-rich region of Mep45 was predicted to be a membrane-bound unit of the 14-stranded β-barrel structure. Here we propose that PG-cadaverine possesses functional importance to facilitate the structural linkage between peptidoglycan and the outer membrane via specific interaction with the SLH domain of Mep45.Journal of bacteriology 11/2010; 192(22):5953-61. · 2.69 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Paenibacillus sp. W-61 is capable of utilizing water-insoluble xylan for carbon and energy sources and has three xylanase genes, xyn1, xyn3, and xyn5. Xyn1, Xyn3, and Xyn5 are extracellular enzymes of the glycoside hydrolase (GH) families 11, 30, and 10, respectively. Xyn5 contains several domains including those of carbohydrate-binding modules (CBMs) similar to a surface-layer homologous (SLH) protein. This study focused on the role of Xyn5, localized on the cell surface, in water-insoluble xylan utilization. Electron microscopy using immunogold staining revealed Xyn5 clusters over the entire cell surface. Xyn5 was bound to cell wall fractions through its SLH domain. A Deltaxyn5 mutant grew poorly and produced minimal amounts of Xyn1 and Xyn3 on water-insoluble xylan. A Xyn5 mutant lacking the SLH domain (Xyn5DeltaSLH) grew poorly, secreting Xyn5DeltaSLH into the medium and producing minimal Xyn1 and Xyn3 on water-insoluble xylan. A mutant with an intact xyn5 produced Xyn5 on the cell surface, grew normally, and actively synthesized Xyn1 and Xyn3 on water-insoluble xylan. Quantitative reverse transcription-PCR showed that xylobiose, generated from water-insoluble xylan decomposition by Xyn5, is the most active inducer for xyn1 and xyn3. Luciferase assays using a Xyn5-luciferase fusion protein suggested that xylotriose is the best inducer for xyn5. The cell surface Xyn5 appears to play two essential roles in water-insoluble xylan utilization: (i) generation of the xylo-oligosaccharide inducers of all the xyn genes from water-insoluble xylan and (ii) attachment of the cells to the substrate so that the generated inducers can be immediately taken up by cells to activate expression of the xyn system.Journal of bacteriology 02/2010; 192(8):2210-9. · 2.69 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Particular Bacillus subtilis strains produce a capsular polypeptide poly-gamma-glutamate (gamma-PGA) that functions as a physical barrier against bacteriophage infection. Bacteriophage PhiNIT1 can infect B. subtilis and produces a novel gamma-PGA hydrolase PghP. PghP was overexpressed, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 2.4 A using a synchrotron X-ray source and were found to belong to space group P3(1)21 or P3(2)21.Acta Crystallographica Section F Structural Biology and Crystallization Communications 10/2009; 65(Pt 9):913-6. · 0.57 Impact Factor -
Article: Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
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ABSTRACT: An industrial strain of Bacillus subtilis (natto) was used to produce poly-gamma-DL-glutamate (gammaPGA), a polymer of DL-glutamate linked by a gamma-peptide bond. In spite of efforts to improve gammaPGA production by modifying the medium, little attention has been paid to the expression of the gammaPGA synthetase gene. In this study, we investigated the expression of the gammaPGA synthetic gene and the gammaPGA product under various conditions with the LacZ-fusion of the synthetic gene (pgsB-lacZ). The 5' upstream regulatory region of the pgsB gene was also investigated by constructing deletion mutations of lacZ-fusion. The pgsB-lacZ was clearly expressed in the early stationary phase and was abolished by degU gene disruption. The results showed that pgsB-lacZ expression was repressed in rich media, and that gammaPGA production was limited by the substrate supply rather than by the amount of synthetase. Adding D-glutamate to the medium reduced gammaPGA production and synthetic gene expression. The transcription start point was determined by primer extension, and it was found that up to -721 bp (translation start point = +1) of the 5' untranslated region (UTR) was required for optimal pgsB-lacZ fusion gene expression.Bioscience Biotechnology and Biochemistry 06/2009; 73(5):1149-55. · 1.27 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Leukocidin (Luk), an exotoxin of Staphylococcus aureus consisting of LukF and LukS, is a hetero-oligomeric pore-forming cytolytic toxin toward human and rabbit polymorphonuclear leukocytes. However, it is uncertain how Luk affects the host immune response. In the present study, we investigated whether Luk has the ability to stimulate mouse bone marrow-derived myeloid dendritic cells (BM-DCs). LukF activated BM-DCs to generate IL-12p40 mRNA, induce intracellular expression and extracellular secretion of this cytokine and express CD40 on their surface, whereas LukS showed a much lower or marginal ability in the activation of BM-DCs than its counterpart component. Similarly, TNF-alpha was secreted by BM-DCs upon stimulation with these components. Combined addition of these components did not lead to a further increase in IL-12p40 secretion. IL-12p40 production caused by LukF was completely abrogated in BM-DCs from TLR4-deficient mice similarly to the response to lipopolysaccharide (LPS). Polymixin B did not affect the LukF-induced IL-12p40 production, although the same treatment completely inhibited the LPS-induced response. Boiling significantly inhibited the response caused by LukF, but not by LPS. Finally, in a luciferase reporter assay, LukF induced the activation of NF-kappaB in HEK293T cells transfected with TLR4, MD2 and CD14, whereas LukS did not show such activity. These results demonstrate that LukF caused the activation of BM-DCs by triggering a TLR4-dependent signaling pathway and suggests that Luk may affect the host inflammatory response as well as show a cytolytic effect on leukocytes.Microbes and Infection 02/2009; 11(2):245-53. · 2.73 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Paenibacillus sp. strain W-61, which can utilize xylan as the sole source of carbon and energy, produces extracellular xylanases 1 and 3 (Xyn1 and Xyn3) and cell surface xylanase 5. In this study we found that lppX, immediately downstream of xyn1, encodes a lipoprotein located on the outer layer of the cytoplasmic membrane and that the LppX lipoprotein is essential for the secretion of active Xyn1 across the cytoplasmic membranes. In Escherichia coli, wild-type LppX was destined for the inner layer of the outer membrane. Mutant LppX(C19A), in which Cys-19, a possible lipomodification residue, is replaced with Ala, was located in the periplasm without being anchored to the membranes. Another mutant, LppX(S20D S21D), with substitutions of Asp for Ser-20 and Ser-21 (conversion to an Asp-Asp signal for sorting to the inner membrane), resided on the outer layer of the inner membrane, demonstrating that LppX has the sorting property of a lipoprotein. E. coli harboring both xyn1 and lppX secreted active Xyn1 into the periplasm. In contrast, E. coli carrying xyn1 alone failed to do so, accumulating inactive Xyn1 in the cytoplasmic membranes. Exogenous LppX(C19A) liberated the inactive Xyn1, which had been stagnating in the inner membrane, into the medium as an active enzyme. Thus, we propose that LppX is a novel type of lipoprotein that assists Xyn1 in making the proper fold necessary for traveling across the cytoplasmic membranes to be secreted as an active enzyme.Journal of bacteriology 01/2009; 191(5):1641-9. · 2.69 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Pectobacterium carotovorum subsp. carotovorum strain Er simultaneously produces the phage tail-like bacteriocin carotovoricin (Ctv) and pectin lyase (Pnl) in response to DNA-damaging agents. The regulatory protein RdgB of the Mor/C family of proteins activates transcription of pnl through binding to the promoter. However, the optimal temperature for the synthesis of Ctv (23 degrees C) differs from that for synthesis of Pnl (30 degrees C), raising the question of whether RdgB directly activates ctv transcription. Here we report that RdgB directly regulates Ctv synthesis. Gel mobility shift assays demonstrated RdgB binding to the P(0), P(1), and P(2) promoters of the ctv operons, and DNase I footprinting determined RdgB-binding sequences (RdgB boxes) on these and on the pnl promoters. The RdgB box of the pnl promoter included a perfect 7-bp inverted repeat with high binding affinity to the regulator (K(d) [dissociation constant] = 150 nM). In contrast, RdgB boxes of the ctv promoters contained an imperfect inverted repeat with two or three mismatches that consequently reduced binding affinity (K(d) = 250 to 350 nM). Transcription of the rdgB and ctv genes was about doubled at 23 degrees C compared with that at 30 degrees C. In contrast, the amount of pnl transcription tripled at 30 degrees C. Thus, the inverse synthesis of Ctv and Pnl as a function of temperature is apparently controlled at the transcriptional level, and reduced rdgB expression at 30 degrees C obviously affected transcription from the ctv promoters with low-affinity RdgB boxes. Pathogenicity toward potato tubers was reduced in an rdgB knockout mutant, suggesting that the RdgAB system contributes to the pathogenicity of this bacterium, probably by activating pnl expression.Applied and Environmental Microbiology 09/2008; 74(19):6017-25. · 3.95 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: The insertion sequence IS4Bsu1 frequently causes Bacillus subtilis starters for the production of Japanese fermented soybean pasts (natto) to lose the ability to produce poly-gamma-glutamate, the viscous material characteristic of natto. Bacillus subtilis NAFM5, a derivative of a natto starter, has six IS4Bsu1 copies on its chromosome. In this study, we determined all six insertion loci of the insertion sequence (IS). One was located in the coding region of yktD, a putative gene involved in polyketide synthesis. Four were located in non-coding regions between iolR and iolA, between tuaA and lytC, between rapI and orf1 (a potential gene of unknown function), and between ynaE and orf3 (a putative gene similar to thiF), and one resided in an intergenic region between divergent possible orf4 and orf5 genes of unknown function. Here we describe the structural features of these loci and discuss the effects of the IS4Bsu1 insertions on the functions of the target gene and the expression of the downstream genes. In addition, we found that strain NAFM5 and commercial natto starters possess eight to 10 loci of another IS of the IS256 family (designated IS256Bsu1) on their chromosomes. IS256Bus1 appeared active in transposition, potentially causing phenotypic alterations in natto starters like those induced by IS4Bsu1.Bioscience Biotechnology and Biochemistry 11/2007; 71(10):2458-64. · 1.21 Impact Factor - Bioscience Biotechnology and Biochemistry 01/2007; 71(10):2458-2464. · 1.21 Impact Factor
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ABSTRACT: Nutritionally versatile Pseudomonads effectively utilize various nitrogenated compounds, including amino acids (such as glutamate, proline, arginine and histidine) as sources of carbon, nitrogen and energy. The histidine catabolic pathway of Pseudomonas was first established in Pseudomonas fluorescens followed by Pseudomonas putida and Pseudomonas aeruginosa. The genes for the pathway enzymes were then identified and characterized in P. putida. Expression of the P. aeruginosa and P. putida histidine catabolic enzymes absolutely requires the presence of histidine (or its degradation intermediate urocanate) and is subject to regulation by both carbon and nitrogen, as in Klebsiella aerogenes (formerly Aeromonas aerogenes) and Bacillus subtilis (for reviews, see refs [9,24,57,85]).12/2006: pages 371-395; - [Show abstract] [Hide abstract]
ABSTRACT: Pseudomonas aeruginosa PAO1 has two possible catabolic pathways of spermidine and spermine; one includes the spuA and spuB products with unknown functions and the other involves spermidine dehydrogenase (SpdH; EC 22.214.171.124) encoded by an unknown gene. The properties of SpdH in P. aeruginosa PAO1 were characterized and the corresponding spdH gene in this strain identified. The deduced SpdH (620 residues, calculated Mr of 68,861) had a signal sequence of 28 amino acids at the amino terminal and a potential transmembrane segment between residues 76 and 92, in accordance with membrane location of the enzyme. Purified SpdH oxidatively cleaved spermidine into 1,3-diaminopropane and 4-aminobutyraldehyde with a specific activity of 37 units (mg protein)(-1) and a Km value of 36 microM. The enzyme also hydrolysed spermine into spermidine and 3-aminopropanaldehyde with a specific activity of 25 units (mg protein)(-1) and a Km of 18 microM. Knockout of spdH had no apparent effect on the utilization of both polyamines, suggesting that this gene is minimally involved in polyamine catabolism. However, when spdH was fused to the polyamine-inducible promoter of spuA, it fully restored the ability of a spuA mutant to utilize spermidine. It is concluded that SpdH can perform a catabolic role in vivo, but P. aeruginosa PAO1 does not produce sufficient amounts of the enzyme to execute this function.Microbiology 09/2006; 152(Pt 8):2265-72. · 2.84 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Pseudomonas aeruginosa PAO1 utilizes 3-guanidinopropionate (3-GP) and 4-guanidinobutyrate (4-GB), which differ in one methylene group only, via distinct enzymes: guanidinopropionase (EC 126.96.36.199; the gpuA product) and guanidinobutyrase (EC 18.104.22.168; the gbuA product). The authors cloned and characterized the contiguous gpuPAR genes (in that order) responsible for 3-GP utilization, and compared the deduced sequences of their putative protein products, and the potential regulatory mechanisms of gpuPA, with those of the corresponding gbu genes encoding the 4-GB catabolic system. GpuA and GpuR have similarity to GbuA (49 % identity) and GbuR (a transcription activator of gbuA; 37 % identity), respectively. GpuP resembles PA1418 (58 % identity), which is a putative membrane protein encoded by a potential gene downstream of gbuA. These features of the GpuR and GpuP sequences, and the impaired growth of gpuR and gpuP knockout mutants on 3-GP, support the notion that GpuR and GpuP direct the 3-GP-inducible expression of gpuA, and the uptake of 3-GP, respectively. Northern blots of mRNA from 3-GP-induced PAO1 cells revealed three transcripts of gpuA, gpuP, and gpuP and gpuA together, suggesting that gpuP and gpuA each have a 3-GP-responsible promoter, and that some transcription from the gpuP promoter is terminated after gpuP, or proceeds into gpuA. Knockout of gpuR abolished 3-GP-dependent synthesis of the transcripts, confirming that GpuR activates transcription from these promoters, with 3-GP as a specific co-inducer. The sequence conservation between the three functional pairs of the Gpu and Gbu proteins, and the absence of gpuAPR in closely related species, imply that the triad gpu genes have co-ordinately evolved from origins common to the gbu counterparts, to establish an independent catabolic system of 3-GP in P. aeruginosa.Microbiology 01/2006; 151(Pt 12):4055-62. · 2.84 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: During early stationary phase, Bacillus subtilis NAFM5 produces capsular poly(gamma-glutamic acid) (gammaPGA, 2x10(6) Da), which contains D- and L-glutamate, and then degrades it during late stationary phase. The gamma-glutamyltransferase (EC 188.8.131.52; GGT) of this strain successively hydrolysed gammaPGA from the amino-terminal end, to yield both D- and L-glutamate. This enzyme was specifically synthesized during the stationary phase through transcriptional activation of the corresponding ggt gene by the ComQXPA quorum-sensing system. A ggt knockout mutant degraded gammaPGA into 1x10(5) Da fragments, but not any further, indicating that the capsule gammaPGA is first internally degraded by an endo-type of gammaPGA hydrolase into 1x10(5) Da intermediates, then externally into glutamates via GGT. Due to its inability to generate the glutamates from the capsule, the ggt mutant sporulated more frequently than the wild-type strain. The results show that B. subtilis GGT has a powerful exo-gamma-glutamyl hydrolase activity that participates in capsule gammaPGA degradation to supply stationary-phase cells with constituent glutamates.Microbiology 01/2005; 150(Pt 12):4115-23. · 2.84 Impact Factor -
Article: Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis.
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ABSTRACT: Many bacteria, including Escherichia coli, have a unique gene that encodes glutamate racemase. This enzyme catalyses the formation of d-glutamate, which is necessary for cell wall peptidoglycan synthesis. However, Bacillus subtilis has two glutamate racemase genes, named racE and yrpC. Since racE appears to be indispensable for growth in rich medium, the role of yrpC in d-amino acid synthesis is vague. Experiments with racE- and yrpC-knockout mutants confirmed that racE is essential for growth in rich medium but showed that this gene was dispensable for growth in minimal medium, where yrpC executes the anaplerotic role of racE. LacZ fusion assays demonstrated that racE was expressed in both types of media but yrpC was expressed only in minimal medium, which accounted for the absence of yrpC function in rich medium. Neither racE nor yrpC was required for B. subtilis cells to synthesize poly-gamma-dl-glutamate (gamma-PGA), a capsule polypeptide of d- and l-glutamate linked through a gamma-carboxylamide bond. Wild-type cells degraded the capsule during the late stationary phase without accumulating the degradation products, d-glutamate and l-glutamate, in the medium. In contrast, racE or yrpC mutant cells accumulated significant amounts of d- but not l-glutamate. Exogenous d-glutamate utilization was somewhat defective in the mutants and the double mutation of race and yrpc severely impaired d-amino acid utilization. Thus, both racemase genes appear necessary to complete the catabolism of exogenous d-glutamate generated from gamma-PGA.Microbiology 10/2004; 150(Pt 9):2911-20. · 2.84 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Some Bacillus subtilis strains, including natto (fermented soybeans) starter strains, produce a capsular polypeptide of glutamate with a gamma-linkage, called poly-gamma-glutamate (gamma-PGA). We identified and purified a monomeric 25-kDa degradation enzyme for gamma-PGA (designated gamma-PGA hydrolase, PghP) from bacteriophage PhiNIT1 in B. subtilis host cells. The monomeric PghP internally hydrolyzed gamma-PGA to oligopeptides, which were then specifically converted to tri-, tetra-, and penta-gamma-glutamates. Monoiodoacetate and EDTA both inhibited the PghP activity, but Zn(2+) or Mn(2+) ions fully restored the enzyme activity inhibited by the chelator, suggesting that a cysteine residue(s) and these metal ions participate in the catalytic mechanism of the enzyme. The corresponding pghP gene was cloned and sequenced from the phage genome. The deduced PghP sequence (208 amino acids) with a calculated M(r) of 22,939 was not significantly similar to any known enzyme. Thus, PghP is a novel gamma-glutamyl hydrolase. Whereas phage PhiNIT1 proliferated in B. subtilis cells encapsulated with gamma-PGA, phage BS5 lacking PghP did not survive well on such cells. Moreover, all nine phages that contaminated natto during fermentation produced PghP, supporting the notion that PghP is important in the infection of natto starters that produce gamma-PGA. Analogous to polysaccharide capsules, gamma-PGA appears to serve as a physical barrier to phage absorption. Phages break down the gamma-PGA barrier via PghP so that phage progenies can easily establish infection in encapsulated cells.Applied and Environmental Microbiology 06/2003; 69(5):2491-7. · 3.95 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Putrescine can be synthesized either directly from ornithine by ornithine decarboxylase (ODC; the speC product) or indirectly from arginine via arginine decarboxylase (ADC; the speA product). The authors identified the speA and speC genes in Pseudomonas aeruginosa PAO1. The activities of the two decarboxylases were similar and each enzyme alone appeared to direct sufficient formation of the polyamine for normal growth. A mutant defective in both speA and speC was a putrescine auxotroph. In this strain, agmatine deiminase (the aguA product) and N-carbamoylputrescine amidohydrolase (the aguB product), which were initially identified as the catabolic enzymes of agmatine, biosynthetically convert agmatine to putrescine in the ADC pathway: a double mutant of aguAB and speC was a putrescine auxotroph. AguA was purified as a homodimer of 43 kDa subunits and AguB as a homohexamer of 33 kDa subunits. AguA specifically deiminated agmatine with K(m) and K(cat) values of 0.6 mM and 4.2 s(-1), respectively. AguB was specific to N-carbamoylputrescine and the K(m) and K(cat) values of the enzyme for the substrate were 0.5 mM and 3.3 s(-1), respectively. Whereas AguA has no structural relationship to any known C-N hydrolases, AguB is a protein of the nitrilase family that performs thiol-assisted catalysis. Inhibition by SH reagents and the conserved cysteine residue in AguA and its homologues suggested that this enzyme is also involved in thiol-mediated catalysis.Microbiology 04/2003; 149(Pt 3):707-14. · 2.84 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Pseudomonas aeruginosa PAO1 utilizes proline as the sole source of carbon and nitrogen via a bifunctional enzyme (the putA gene product) that has both proline dehydrogenase (EC 22.214.171.124) and pyrroline 5-carboxylate dehydrogenase (EC 22.214.171.124) activities. We characterized the pruR-putAP loci encoding the proline catabolic system of this strain. In contrast to the putA and putP (encoding proline permease) genes of other gram- negative bacteria, which are located at divergent or separate loci, Northern blotting demonstrated that the two genes form an operon in strain PAO1. While the phylogenetic lineage of the PutP protein of strain PAO1 was related to that of the origin (80% identity to the P. putida counterpart), PutA of PAO1 (PutA(PAO)) was rather distantly related (47% identity) to the P. putida counterpart. Moreover, unlike the PutA proteins of P. putida and enteric bacteria, PutA(PAO) appeared to lack a regulatory function. Upstream of the putAP operon, the divergent PA0781 gene specified a hypothetical outer membrane protein with a molecular weight of 74,202. This gene appeared to be dispensable for proline utilization as indicated by the normal growth of a knockout mutant of PA0781 on medium containing proline. The pruR (proline utilization regulator) gene immediately upstream of PA0781 encoded a transcriptional activator of the AraC/XylS protein family and mediated the proline-responsive expression of putAP. Primer extension studies identified a PruR-dependent promoter responsive to proline in the 5'-flanking region of putA. Thus, the proline utilization system of P. aeruginosa differs from that of P. putida with respect to putA structure, the organization of the putAP genes, and the regulatory mechanism of putA expression.Journal of Bacteriology 11/2002; 184(20):5633-40. · 2.69 Impact Factor - [Show abstract] [Hide abstract]
ABSTRACT: Among 45 Bacillus subtilis strains isolated from non-salted types of fermented soybeans produced in several Southeast Asian countries, 20 had the insertion sequence IS4Bsu1 in the chromosome. In contrast, none of 49 B. subtilis strains of non-food origin contained IS4Bsu1. Frequent occurrence of this mobile DNA element in the soybean-fermenting B. subtilis would reflect the fact that few strains flourish on soybeans and thereby contribute to soybean fermentation.Bioscience Biotechnology and Biochemistry 10/2002; 66(9):1994-6. · 1.21 Impact Factor
Publication Stats
955 | Citations | |
107.31 | Total Impact Points | |
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Tohoku Gakuin University
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Сендай, Miyagi, Japan
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Sendai-shi, Miyagi-ken, Japan
Aino University
Takatuki, Ōsaka, Japan
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- Department of Biology
Atlanta, Georgia, United States
Shinshu University
Shonai, Nagano, Japan


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Pew Report Shines Light on Failed Immigration Policy |
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A new report from the Pew Hispanic Center entitled A Rising Share: Hispanics and Federal Crime analyzes the ethnic composition of those sentenced in federal courts. Beneath the startling headline, however, is a familiar story. Immigrants do represent a disproportionate share of the federal prison population because immigration law is under the purview of the federal courts.
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Took a half day morning trip. Caught a lot of fish.Numerous keepers. Even got our names in the paper for one of the fish we caught. The kids are still talking about it. Tom was a great captain.
Took a half day morning trip. Caught a lot of fish.Numerous keepers. Even got our names in the paper for one of the fish we caught. The kids are still talking about it. Tom was a great captain.
Took our two boys on their first ocean fishing trip. They made sure it was special. It was so fun for the whole family. We would definitely recommend them and if we return, we will do it again!
1/2 day afternoon trip for Stripers off of Monomoy. Brought my 3 older teens and one of their friends. We each took a keeper and threw several more back for another day. Great Trip. Tom is an excellent captain in a very busy waterway.
Took the kids and a couple of friends on the 1/2 day trip - NO fish and not even a single bite! It was their first time deep sea fishing and it would have been nice have actually fished for more than 20 minutes out of the 4 hours spent trolling and moving to new spots with no results. Total... More
I understand your frustration with the lack of fish. As Captain/Owner, this sort of trip is the most unfortunate part of being in this business, where there is the potential for the trip to result in no catch. Part of fishing is this potential, however it is rare. I would be happy to make a future accommodation if you were... More
After spending a few minutes on the phone, decided to try out Captain Tom. We arrived at the dock for an afternoon charter with three 9-year old boys . The boat was a a few minutes late but Tom made it up to us on the back end of the trip. The morning fishing trip produced no results but our... More
Four of us went tuna fishing with Tom and Chris. We had a great time! Both Tom and Chris were very friendly and fun to be with. It was SO exciting to catch two large tunas!! In addition we saw tons of dolphins and a few whales which really was awesome. Tom even hooked us up with a great restaurant... More
We took our two young kids out to "try" fishing with Captain Tom--and it was truly wonderful! Tom was professional and had such a kind manner with our kids. We reeled in 5 striped bass--my 8 year old pulled in the largest catch measuring over 41 inches. From beginning to end a very special occasion for kids and grown ups... More
I've been lucky enough to go fishing with Capt. Tom George a couple of times. Once for Stripers and once for Tuna - both times he got us on the fish, and both times we had a blast! Besides being a great fisherman Tom is a real pleaseure to be around on the boat, not pushy at all but gives... More
We went to Chatham to take the Lighthouse tour, which if you like lighthouses you really should do, and went to the fish pier for lunch. The Scallops were to die for.We shared the scallop plate as there are so many one can not eat all of them . We got to see the Seals come up as we watched... More
We went with a Mr. Murdock on a seal tour from the fish pier and saw lots of seals. Trip lasted about 1 hr. We also saw a lot of the beautiful homes along the shore and got a bit of area history as well. Would go again.
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Hi Everyone, I too am researching Swarbrick in the Fylde area, the name is an over the Wyre name. Although some came south of the Wyre to places like Fleetwood etc, many have their root in the Kirkham area. Mine cane from Hambleton, although Jane Swarbrick my Great Grandmother married and lived in Thornton le Fylde and Fleewood....any cousins out there...kindest regards Rosemarie Waring
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ETA to your door on Decals & Emblems Click on the price to add parts to your "Shopping Cart." Click checkout for shipping cost.
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A - Z1 Air/Exh Bolts sets Books Brakes Controls Electrical Engine Fuel Frame Gaskets Ignition Motorcycles Suspension Tuning Windshields _VETTER_ Brackets Parts Shields Vetter Help
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If our shopping cart is not working, click here to order parts directly using your credit card We accept VISA or MasterCard. Also see the links at the bottom of our web pages. New & Used parts are available. Everything we have listed on our site is available. If a part is not listed or does not have a price link next to it, then it is not available. Go here for Decal & Emblem ETA's.
We like to here from our customers, just click the Email link above to send us an email (firstname.lastname@example.com) with any suggestions or comments.
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1980-81 Kawasaki KX420 6pc Fuel Tank, Side Panel, Swing Arm Decal Set KKX-81-420-CS $99.99
Printed on silver backing.
1980-81 Kawasaki KLX250 Complete 4pc Decal Kit KKLX-81-250-CS $74.99
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Printed on silver backing


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Type: Posts; User: ryan391
For Sale a very good condition Boss X. I used it as a backup, I just don't need it.
$120 TYD Paypal.
Once I replaced the batteries, I have not hunted with this sight. I had been using the light for indoor shooting in my basement or practicing field in some pretty dense wooded areas. The sight does...
Padgett has some great points. My personal headache with 22's (have never tried x cutters), is that fat shaft GT's are only built in one spine. For me that is way over spined. I am not a tuner,...
Tru Ball Absolute 360 with V Lock Stap. Just received back from Tru Ball. they went through it and gave me a tune up. They replaced the head and some other internals. Works like new. $150 with v...
The original light lasted forever. I accidentally left it on in my case and burned it out. Since then I have been extra cautious to make sure I check to make sure the light is off. I know I bought...
Why does my sight light only last a few days? I have a spot hogg light and originally it lasted for a season. I replaced the batteries and they only lasted for two weeks.
Do other brands last...
sorry yes it is the wide model. It fits the absolute 360
I am not sure. It is the same size as all the other Truball straps at cabbalas and BP
Sab323 I agree the FMJ is an awesome arrow, but I have not had good experience with them and targets. They hit so hard and penetrate so deep that depending on target material, they either penetrate...
I am not an expert, but I shoot the Velocities for 3d. I do this for a couple of reasons. First, I am have a shorter draw at 27". I want all the speed I can get for Hunter class 3d, longest shot i...
Do you have any pics?
I am not sure what I was expecting. How much better is this than shooting just using the rise.
$40 TYD paypal
Perfect condition, new, no box. Leather is not even creased.
$30 paypal
I'll take one pack. Pm sent
While doing a search for some side plates for my alloy, I ran across a tweet from Shrewd saying they have grips for G5 Pime bows. I did not see them on their web site, so I called. The customer...
where are you in Mo?


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Industrial and Demanding Environments The ABL Industrial National Accounts Group focuses on industrial customers who have a wide variety of processes and environments within their facilities. These environments include standard light and heavy industrial applications including what we at Acuity Brands Lighting define as "Demanding Environments" which include Food Processing and Hazardous/Petro-Chemical facilities.
Critical areas in a Demanding Environment facility, along with light and heavy industrial facilities, require precisely designed luminaires to provide the optimum in plant floor lighting as well as the lowest "Long Term Operating Costs".
By combining ABL's product portfolio of precisely designed luminaires in conjunction with our standard luminaire offering, the ABL National Accounts is uniquely positioned to meet both the short term and long term sustainability within a facility to provide a completely integrated lighting system. The Challenge of "Sustainable Lighting" ABL's industrial customers are increasingly challenged with the need to be stewards of the environment. Real pressures to become 'Green', the catch word that is now driving building design for new and existing plants and facilities, poses new challenges for those who want to address energy concerns.
While many lighting companies are capitalizing on this energy trend, they do so without considering the customers total cost of ownership.
Acuity Brands Lighting embraces the challenge to deliver the best lighting environment through marketing, research and development, and new manufacturing methods to give our customers energy savings and the lowest cost of ownership. Addressing National Regulatory Groups The capabilities' that ABL provides to our customers goes far beyond that of other lighting suppliers. ABL has the luminaire choices and design experience that will help you and your engineering and maintenance teams design a lighting system compliant with:
• Local, State, and Federal Energy codes including EISA and EPAct. • Green Building Standards as established by Green Globes and LEED.
Setting the Bar Industrial and Demanding Environment lighting holds many challenges and one is the need for increased light levels for safety concerns while lowering the Kilowatt usage in the new design. This is where ABL's luminaire technology in Optical Control, Ballast Design and Thermal Management Systems all play a key role in being able to:
• Achieve more light with fewer fixtures or reduced lamp wattages. • Overcome any harsh environmental factors in the space with an appropriately applied luminaire.
In addition to providing lighting design, facility audit services and an economic payback analysis, SAERIS can also furnish:
• Project management • Labor for installation • Disposal of the existing lighting system • Warranty services of a facility's lighting upgrade regardless of its location within the United States.
Financing a lighting project is available for those customers who desire an alternate method to invest in a lighting upgrade rather than utilizing internal capital expenditure funds to support the project.
Controls: The ABL National Accounts Group has the ability to provide state-of-the-art lighting control systems from our ABL companies Synergy, LC&D and Sensor Switch. These products can be engineered into lighting systems providing unparalleled control and maximize energy savings, further reducing your companies carbon footprint. ROAM --
Remote Operations Asset Management: On average 5-10 percent of outdoor pole-mounted light fixtures on a given system are not working at any one time. This results in customers being billed for services they are not receiving, and excessive energy consumption and costs through day-burning light fixtures.
In the past, outdoor lighting system owners and operators were unable to quickly detect, repair and manage problems with their lighting systems. They also could not provide the levels of service expected by the public. Now, with ROAM products and services, you can.
For more information or additional assistance, please contact the ABL National Accounts Group at the phone number or e-mail link listed below.


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192 High Street (1st Floor) West Drayton, Middlesex United Kingdom UB7 7BE |
ITF Events - Click on a headline to view the event detailsThe events listed here are fully sanctioned by the ITF, as such follow a set of strict criteria for the running and promotion. Additional INO / MO events can be found via their organisation profile.
UK English Championships 28th Feb 2015 UK ITF Championships - Torbay Leisure Centre, Paignton ITF Sanctioned Event
Alaska International Instructor Course 22nd Jun 2015 Master Class - Fairbanks, Alaska ITF Sanctioned Event
AFRICAN ITF TAEKWONDO CHAMPIONSHIP 8th Aug 2015 Championships - Addis Ababa, Ethiopia ITF Sanctioned Event


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A man is alleged to have bitten off a rabbi's finger at a public Chanucah celebration in Vienna.
Rabbi Dov Grussman was responding to apparent antisemitic jibes from the Muslim man, who is said to have hurled himself at the Chabad Rabbi and punched him.
During the struggle, the man is alleged to have bitten the rabbi, severing part of his finger. He was later arrested by Austrian police.
Rabbi Grussman was taken to hospital where doctors attempted to reattach the severed finger. He is currently being monitored for infection from the injury.
The Spanish FA will hold a disciplinary hearing for La Liga side Osasuna after Real Mallorca's Israeli goalkeeper Dudu Awat was subject to antisemitic abuse in his side's 1-0 victory at the Navarro stadium in Pamplona on Sunday night.
Referee Alvarez Izquierdo noted in his match report to the Spanish FA that the crowd chanted "Jewish bastard" every time Awat touched the ball, and that the fans only stopped their racist abuse in the second half following five requests over the public announcement system.
The fans waved Palestinian flags and also shouted "murderer" at Awat.
The union that sits on the official Government Group on Antisemitism and Higher Education is facing calls for its expulsion because it hosted a man who made an antisemitic speech.
The University and College Union (UCU) invited Bongani Masuku, international secretary of the Congress of South African Trade Unions (Cosatu), to tour UK universities this week and to speak at an invitation-only conference last Saturday, arguing for a boycott of Israel.
Dmitriy Salita, the previously unbeaten Jewish boxer who was floored by Muslim fighter Amir Khan in just 76 seconds on Saturday night, said this week that antisemitic chanting may have cost him the fight.
Speaking exclusively to the JC, Salita, who won his previous 31 bouts, said: "It was over before it started. I was in great shape and well prepared but the antisemitic chanting left me completely overwhelmed."
And here are the results: Football 1, antisemitism 0. Boxing 0, antisemitism 1. That has been the outcome of two major sporting events in the UK over the past two weeks.
It's clear from this that sport and politics do not mix. Sport has been used as a platform by organisations and countries to protest and vent their anger towards political situations, but this reached new heights on Saturday night when Dmitriy Salita experience race hate walking into the ring.
Antisemitic incidents in Australia have reached record levels, although the number of physical assaults on Jews have dropped, according to an annual report.
Jeremy Jones, who has been collating data on antisemitic incidents in the country since 1989, told the annual conference of the Executive Council of Australian Jewry (ECAJ) on Sunday that an unprecedented 962 reports of anti-Jewish violence, vandalism, harassment and intimidation were received by Australian Jewish organisations between October 1, 2008 and September 30, 2009.
A senior diplomat convicted of racially aggravated harassment for a foul-mouthed anti-Israeli rant in a gym has been given a new role at the Foreign Office.
Rowan Laxton, 48, the then head of the South Asia desk at the FO, was exercising in a gym when saw a television report during Israel's action in Gaza and shouted "f------ Israelis, f------ Jews".
Two men exercising elsewhere overheard him, complained to staff and Mr Laxton was arrested and charged subsequently. He was also suspended from his post at the FO.
The US State Department has appointed a new special envoy to monitor and combat global antisemitism.
Hannah Rosenthal, 58, a former director of the Jewish Council for Public Affairs, will assume the post, which was created by Congress in 2004.
Ms Rosenthal's appointment has already been met with some scepticism by conservative Jews because of her past criticism of the "narrow, ultra-conservative views" that dominate American pro-Israel groups.
Comments made by the former British ambassador to Libya, Sir Oliver Miles, have been called "unacceptable" and "antisemitic" after he criticised the appointment of two leading Jews to the Iraq inquiry.
Sir Oliver drew attention to the appointment of Holocaust historian and Winston Churchill biographer Sir Martin Gilbert, and the war historian and "Blair doctrine" architect Sir Lawrence Freedman, who advocated humanitarian intervention in Kosovo and Afghanistan.
The two men have been made privy counsellors in order to sit on the inquiry committee, which opened this week.
Students at a Florida school have been suspended for participating in 'Kick a Jew' Day.
Ten pupils at North Naples Middle School were suspended for a day, after a female student told the school's head that she believed she had been kicked as part of 'Kick a Jew Day'.
Local expressed concerns that the students may have been inspired by TV show South Park where an episode featured 'Kick a Ginger Day'. The attacks are believed to have been co-ordinated via a Facebook group.


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Healthcare Providers and Services
Company Overview of Capital Blue Cross
Executive Profile
James M. Mead BS, MASecond Vice Chairman and Special Advisor to the Chief Executive Officer, Capital Blue Cross |
Age | Total Calculated Compensation | This person is connected to 9 Board Members in 9 different organizations across 7 different industries. See Board Relationships |
68 | -- |
Corporate Headquarters2500 Elmerton Avenue Phone: 717-541-7000Harrisburg, Pennsylvania 17177 United States Fax: 717-541-6915 Board Members MembershipsSecond Vice Chairman and Special Advisor to the Chief Executive Officer Director Director Former Director Director Director Director Director Director 2011-Present Independent Director, Member of Audit Committee and Member of Governance Committee
EducationMA Pennsylvania State University BS Pennsylvania State University
Other Affiliations |
Annual CompensationThere is no Annual Compensation data available.Stocks OptionsThere is no Stock Options data available.Total CompensationThere is no Total Compensation data available. |
There is no Competitor Compensation data available. |
Sponsored Financial Commentaries
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To contact Capital Blue Cross, please visit . Company data is provided by Capital IQ. Please use this form to report any data issues.
Our data partners will research the update request and update the information on this page if necessary. Research and follow-up could take several weeks. If you have questions, you can contact them at email@example.com.


text:
SKN Vibes article.
BASSETERRE, St. Kitts - The West Indies Cricket Board and the University of Technology in Jamaica have partnered to revamp the WICB Coaching Programme.
WICB Media Release.
KINGSTON, Jamaica – The West Indies Cricket Board has showered praise on the West Indies Women's team following their superb 2-1 series win over New Zealand Women at Sabina Park.
BASSETERRE, St. Kitts – Beddecked in their new WICB Grass Roots Cricket shirts and caps, over 80 young cricketers turned up on Saturday (Sept. 28) at the St. Paul's Cricket Complex to participate in the end of the Quarter Cluster Matches.
Kingston, Jamaica – The West Indies Cricket Board today announced that the Regional Super50 Tournament has been awarded to Trinidad and Tobago for three years.


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Connecticut's attorney general files a lawsuit against CVS Pharmacy
Connecticut's attorney general Richard Blumenthal has filed a lawsuit against CVS Pharmacy Inc. for allegedly selling expired food, beverages and over-the-counter medications.
As per attorney general's office, investigators found about 20 of 45 Connecticut CVS Pharmacy stores selling products past their expiration dates.
Expired products found in the Connecticut locations include baby formula, cough medicine, antacids, energy drinks, yogurt, eggs and milk.
But, when contacted, CVS spokeswoman Joanne Dwyer said, "The health and safety of our customers is our top priority."
The lawsuit, which is filed in state superior court in Hartford, seeks significant money penalties for violation of the state consumer protection laws. It also seeks an order barring CVS Pharmacy from selling out-of-date products.
According to the lawsuit, CVS Pharmacy is liable for civil penalties of up to $5,000 per violation.


text:
Mario Party Party 3
Become Premium to Watch
The chaos and fun of Saints Row is fully intact in the underworld, but a new setting can only go so far.
Dimensions recaptures enough of Geometry Wars' thrills to stand as the best new dual-joystick shooter in quite a while.
The little mushroom man finally gets his name on the marquee, and the result is a charming, unique, and puzzle-heavy adventure.
Company »
The main character from the Last Ninja series
The son of Odin, Thor is the god of thunder in Norse mythology. He wields the mighty hammer Mjolnir and at Ragnarok he must fight the world serpent Jormundgand.
Everyone's favorite ghost-chasing, power-pellet-eating, dot-hunting yellow pie-shaped blob.
He is a wizard and sells Flimbo certain items to help him through the game.
Mad scientist. He created a machine that draws the life energy from its victim for him. So he kidnapped flimbos girlfriend Pearly to use his machine on.
Flimbos girlfriend. Was kidnapped by the mad scientist Franzs Dandruff.
Hero of the game Flimbo's Quest. He sets out to save his girlfriend Pearly who was kidnapped by the mad scientist Franzs Dandruff.
Medusa is often portrayed in games as a creature that is a cross between a woman and a snake. The lower body is often a snake tail, and the hair is usually a mass of snakes. Other than this, the head and torso are that of a woman.
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Patriots Notebook
GREEN BAY, Wis. -- Defensive lineman Richard Seymour expressed remorse after yesterday's victory over the Packers, confirming a CBS television report that he was fined $7,500 for stepping on Colts offensive tackle Tarik Glenn in a game Nov. 5. (Full article: 1149 words)
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On October 26th, a hole was blasted in the base of 125' tall Condit Dam on the White Salmon River in Washington. In less than 2 hours, the reservoir behind the…
Everybody knows that Stumbleupon is an great source for beautiful photography, nature, pets, arts and much more. They have millions of users and they are
Amazing places that you should see at list once in your life. 1. Wuhua Hai lake or lake 5 flowers. National Park Jiuzhaigon in
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a picture is worth a thousand words


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Ventura Sculptra Doctors and Reviews
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Ventura Sculptra cost
Average cost in US: $1,775
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Ventura Sculptra reviews
100% of Ventura reviewers said Sculptra was worth it
"It Takes Time to Work - Ventura, CA"
I am a 72 year old woman and it was suggested by my dermatologist that I try Sculptra for the lines on my chin and the hollows of my cheeks. I had three injections over four months.It was very discouraging because I did not see any results. I thought I had just thrown $2400 down the drain. It took about eight months before the my body started to react with the Sculptra. The skin on my chin... more
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News from Upstate
August 10, 2012
Darryl Geddes 315 464-4828
Darryl Geddes 315 464-4828
Upstate Medical University medical students receive their white coats during ceremony Aug. 9
Incoming students at Upstate's College of Medicine received their white coats to symbolize the beginning of their medical education, during a ceremony Aug. 9. White coat ceremonies are a rite of passage at medical schools across the country. Upstate will hold white coat ceremonies for incoming students in the College of Nursing Aug. 22 and College of Health Professions Aug. 29.
Search Upstate News
Upstate in the News
- Schumer: Feds should pay $1.3 million bill for Ebola response at Upstate University Hospital
Syracuse Post Standard
- Paige's Butterfly Run raised a record $224,000 in 2014 for Upstate Golisano Children's Hospital
Syracuse Post Standard
- Your 'living will': What happens if you change your mind?
Market Watch
- New study says more than half of U.S. babies sleep in unsafe conditions
WSYR TV9 Syracuse
- Upstate doctor: Why we still need World AIDS Day (Commentary)
Syracuse Post Standard
- Upstate expects to screen more smokers for lung cancer now that Medicare will pay for test
Syracuse Post Standard


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Los Angeles Kings
Panthers Continue Road Trip Against Struggling Sharks
The Florida Panthers had a very hard time scoring goals during the first few weeks of the season.
Kings Cage Panthers, 5-2
Matt Greene started the Los Angeles Kings' big second period by swooping in on net for a slick goal. Robyn Regehr finished it with a booming slap shot for another score.
Panthers Continue Road Trip Against Defending Champ Kings
The Florida Panthers are becoming one of the more well-rounded teams in the NHL.
Panthers Start West Coast Road Swing In Anaheim
The Florida Panthers are back on the road, starting a west coast swing with a Sunday matchup against the Western Conference's top team.
Mitchell Already A Leader For The Florida Panthers
The "C" stands for captain. In Willie Mitchell's case, it may as well stand for Cup.
Rejuvenated Panthers Ready To Start Winning
There are reasons to believe that things will be different in Sunrise this season.
'Miracle On Ice' Member Bob Suter Dies At 57
Bob Suter, a member of the "Miracle On Ice" team that won the Olympic gold medal in 1980 and the father of Minnesota Wild star Ryan Suter, died Tuesday at the age of 57.
Baseball Hall Of Fame Enshrines Six New Members
Frank Thomas choked back tears, Joe Torre apologized for leaving people out of his speech and Tony La Russa said he felt uneasy.
Panthers Name Kelly, Morris As Gallant's Assistants
The Florida Panthers have solidified their coaching staff and will employ four assistant coaches to work under new head coach Gerard Gallant.
Kings Defeat Rangers In Double OT To Win Stanley Cup
Alec Martinez scored 14:43 into the second overtime, and the Los Angeles Kings won the Stanley Cup for the second time in three years with a 3-2 victory over the New York Rangers in Game 5 on Friday night.


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could God WANT you to be led by a slaver and a butcher?
Submitted by FreedomsReigning on Sun, 03/24/2013 - 22:50. Permalink
Don't think of it as me judging your words, but rather whether they're "your words" or Romans 13. I don't see anything in there about your hierarchy of submissions; "God, family, community then government."
I guess it boils down to this spin on it for me; could God WANT you to be led by a slaver and a butcher, and if you find yourself led by such a man, what should you do?
7 Render therefore to all their dues: tribute to whom tribute is due; custom to whom custom; fear to whom fear; honour to whom honour.
8 Owe no man any thing, but to love one another: for he that loveth another hath fulfilled the law.
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text:
Gift Guide 2014: Five NYC-Based Healthy Cookbooks
New Year's is quickly approaching, which means lofty resolutions are about to commence. After months of indulging on baked goods and booze, chances are there's someone in your life who's going to attempt to turn over a nutritious new leaf starting on January 1 -- and you probably already know who it's going to be. Push them ahead with the gift of nutritious recipes. Here are the five best new healthy cookbooks, from NYC-based authors.Emeraldhorticulture for Wikimedia Commons


text:
OSDDMalaria:Arylpyrazole Series
From OpenWetWare
Aryl Pyrazole Sub-series
The OSDDMalaria pyrazole series is derived from the OSDDMalaria GSK arylpyrrole set.
ChEMBL-NTD TCAMS data set has 89 hits with aryl-pyrazole core; 18 hits with an 5-alkyl aryl pyrazole core; 14 with activity below 500 nanomolar:
- TCMDC-131674, CID44522996: pXC50 3D7 147 nM ACD LogP 4.55
- TCMDC-123909: pXC50 3D7 234 nM ACD LogP 3.18
- TCMDC-124020: pXC50 3D7 253 nM ACD LogP 3.03
- TCMDC-124032: pXC50 3D7 258 nM ACD LogP 4.06
Other compounds of interest from PubChem:
- CID3568222. Commercially available. Has been assayed for antimalarial activity AID504832, data submitted by David Fidock
The core can be synthesised with apparent ease from ethylacetoacetate, DMF dimethyl acetal and phenylhydrazine.


text:
Browsing the Internet is more important than ever to handset users, developers and operators. There is not just a battle for operating systems taking place, but also a war for Web browsers.
Our latest report, Global Handset Browser Marketshare in 2014 -- from our Wireless Device Strategies (WDS) research service-- tracks global mobile browser marketshare for all mobile phones shipped worldwide during 2014, including Opera, Safari, Chrome and others.
The report is available to download here to clients.
In a newly published report Strategy Analytics identifies and analyzes the ten most important trends that will impact the mobile device industry in 2015. The report examines a range of key topics including online handset distribution, in which price tiers the most rapid growth will come from, accelerating handset markets, and technologies including LTE-Advanced, SIM cards, and contextually aware services.
Clients of Strategy Analytics' Wireless Device Strategies service can access the report Top 10 Mobile Device Trends and Recommendations for 2015 here.
Even as its share in the North American market steadily shrinks, Huawei's global handset shipments rose +15% annually during Q3 2014. Most of Huawei's cellphone volumes now ship into developing regions like Africa, and we note the Chinese vendor is quietly evolving into an "emerging markets specialist". Even as Huawei focuses more of its attention on emerging markets, it is managing to steadily increase ASPs, in part by focusing on phablets. However, by concentrating on emerging markets at the expense of growth in developed ones such as Western Europe, Huawei risks ceeding market opportunties to Chinese vendors like Lenovo, ZTE, and others.
The report Q3 '14 Huawei Emerging Market Specialist, available to clients of Strategy Analytics' Wireless Device Strategies service, examines Huawei's Q3 2014 performance and strategic focus.
Global shipments of handsets grew by 8% year-on-year in Q3 2014, propelled by a rapidly expanding LTE market in Asia and North America. Apple led the LTE category, with its newly introduced iPhone 6 and 6 Plus models, while Lenovo ranked first in TD-SCDMA, even as that category shrank.
The report Vendor Share Global Handset Market by Technology Q3 2014 provides worldwide quarterly handset shipments for more than 30 vendors by nine major 2G, 3G and 4G protocols. Clients of our Wireless Device Strategies service can access the report here.
According to our WDS (Devices) service, LG, of South Korea, grew its global mobile phone shipments by +19% YoY in Q3 2014, the highest level for three years. Volumes were bolstered by solid demand for its premium- G3 and low-mid-tier L-series III products. Now that LG's strategy of focusing on the G series flagships in North America and elsewhere is paying off, LG MUST turn its attention to the China and India markets where it continues to lag. Additional analysis of LG in the global and regional mobile phone market can be downloaded by clients here.
LTE handset sales are set to surge in 2015 according to new research from our WDS (Devices) service. China and the US will drive sales growth, with global LTE handset sales expanding by a healthy 37% year-on-year in 2015, making the technology the fastest growing and most important segment in the mobile phone market. The big four LTE markets will be China, the US, South Korea and Japan, with growth in China driven by a rapid transition from 2G/3G to LTE, and in the other three markets by a transition to next generation LTE-A which will support advanced new services. This report forecasts LTE handset sales for 88 countries through 2020 and is an essential resource for operators, software developers, content developers, handset vendors, component makers, car manufacturers and other stakeholders to determine or prioritize the size and growth rate of the huge 4G phone market.
Lenovo's global handset shipments grew +40% annually and delivered record marketshare in Q2 2014. Firmly planted among the top-10 global handset vendors, the Chinese company is poised to enter the top-5 if the Motorola acquisition gets approved by authorities in the second half of the year. However, despite record shipments, growth is slowing for Lenovo, profits remain elusive, and the takeover of loss-making Motorola will make achieving profitability that much harder in the near-term.
The company's sales volumes are strong in Central and Eastern Europe (CEE) and particularly so in the Middle East and Africa (MEA). Lenovo smartphones were available in 20 markets in CEE and MEA in Q2 2014 and it is entering +20 additional markets in the regions from 2H 2014.
For the present Lenovo is holding off expanding in Western European markets as it waits to complete its acquisition of Motorola. It intends to leverage the Motorola brand, which has strong recognition and 90% consumer brand awareness to target the region.
Lenovo believes that a market shift from premium to mid-tier devices favors its portfolio, and also expects to realize economies of scale as it leverages its supply chain for Motorola handset production. However, profitability will continue to be impacted for at least four to six quarters as it integrates Motorola into business.
According to the latest research from our Wireless Device Strategies (WDS) service, global mobile phone shipments grew 5 percent annually to reach a record 1.7 billion units in 2013. TCL-Alcatel became the world's fifth largest mobile phone vendor for the first time ever in the final quarter of the year.
Despite ongoing economic headwinds in Asia and other emerging markets, global mobile phone shipments managed to grow a respectable 5 percent annually from 1.6 billion units in 2012 to 1.7 billion in 2013. It was the industry's strongest overall performance for two years.
Fuelled by robust demand for its popular Galaxy models, Samsung tightened its grip, shipping a record 451.7 million mobile phones worldwide and capturing 27 percent marketshare to solidify its first-place lead. If Samsung maintains its current growth rate, it could ship a half-billion mobile phones in 2014.
Nokia's global mobile phone shipments fell 25 percent from 335.6 million units in 2012 to 252.4 million in 2013. Nokia faced tough competition from Samsung in developing markets like India, while LG and others ramped up the pressure in developed regions such as Western Europe. Nokia's Windows Phones have been performing relatively well, but this was not enough to offset sluggish demand for its Asha models and other feature phones during the course of the year.
Apple shipped a record 153.4 million mobile phones worldwide in 2013, up from 135.8 million in 2012. However, Apple's growth rate moderated from 46 percent in 2012 to just 13 percent during 2013. Apple's lack of presence in the low-end smartphone segment and the big-screen phablet category are costing the firm sizeable volumes.
Exhibit 1: Global Mobile Phone Vendor Shipments and Market Share in Q4 2013 [1]
Global Mobile Phone Vendor Shipments (Millions of Units)
Global Mobile Phone Vendor Marketshare %
Total Growth Year-over-Year %
Source: Strategy Analytics
The full report, Global Handset Shipments Grow 5 Percent in 2013, is published by our Wireless Device Strategies (WDS) service, details of which can be found here.
[1] Numbers are rounded. The data-table does not include grey phone shipments. The Mobile Phone total is defined as smartphones plus feature phones combined.
We recently spoke about new emerging technologies that will drive device sales in coming quarters and years. As competition between smartphone vendors grows ever more heated, handset makers are looking to new technologies to differentiate them from their competitors and to capture the attention of consumers. From finger-print readers and Bluetooth Smart to Miracast and wearable accessories, the leading smartphone makers in the second half of 2013 are introducing new technologies that they hope will separate them from their competitors. Successful new enabling technologies introduced in 2H 2013 by Apple, Samsung, Nokia and other leaders will be the templates that fast followers will adopt in 2014. These issues, along with forward-looking shipment and revenue forecasts for 2H 2013 were discussed in our exclusive Webinar. The webinar is available for replay for Strategy Analytics clients.
The impending avalanche of NFC phones, which our Wireless Device Strategies (WDS) service projects to grow at an average of 67% per year over the next five years, has everybody thinking about contactless payments. With all the buzz around Google's soft-launched Wallet service and the US carrier joint venture ISIS, which should roll out in 2012, it makes sense. Indeed, the simple fact that money is directly involved in this particular application of NFC rightfully encourages the whole wireless value chain to think about potential revenue opportunities.
However, there is one often overlooked application for NFC -- intelligent device pairing. The idea is simple: instead of inputting PINs, passkeys, or even 26 hexadecimal digits to pair two wireless devices, the user simply "taps" two NFC devices together. The concept can be applied for any pairing event, regardless of which enabling technology, such as Wi-Fi or Bluetooth, is used to make the actual connection.
So far, only one handset vendor has actively promoted NFC for this application. Nokia's latest NFC-enabled handsets and Bluetooth headsets can be paired together using this very concept. Unfortunately, the latest Windows Lumia devices are not yet in this category, as Microsoft has not yet added NFC support to its platform. Nevertheless, Nokia's attention to NFC tech is a positive sign for the vendor's future portfolio. Nokia's strategy holds two key benefits: it future-proofs handsets, getting them ready for mobile contactless payment services once they eventually roll out, and it improves the usability of a typically cumbersome process.
Chip supplier Broadcom, whose interests span Bluetooth, Wi-Fi, and NFC has also recognized this useful application of the emerging tech, and we expect its chipsets and middleware to help device vendors think beyond mobile payments as they develop their NFC smartphones and tablets.
Alex SpektorWireless Device Strategies


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Summary: Raymond Aron's 1955 masterpiece The Opium of the Intellectuals, is one of the great works of twentieth- century political reflection. Aron shows how noble ideas can slide into the tyranny of "secular religion" and emphasizes how political thought has the profound responsibility of telling the truth about social and political reality-in all its mundane imperfections and tragic complexities.
Aron explodes the three "myths" of radical thoug ...show moreht: the Left, the Revolution, and the Proletariat. Each of these ideas, Aron shows, are ideological, mystifying rather than illuminating. He also provides a fascinating sociology of intellectual life and a powerful critique of historical determinism in the classically restrained prose for which he is justly famous.
For this new edition, prepared by Daniel J. Mahoney and Brian C. Anderson as part of Transaction's ongoing "Aron Project," political scientist Harvey Mansfield provides a luminous introduction that underscores the permanent relevance of Aron's work. The new edition also includes as an appendix "Fanaticism, Prudence, and Faith," a remarkable essay that Aron wrote to defend Opium from its critics and to explain further his view of the proper role of political thinking. The book will be of interest to all students of political theory, history, and sociology. ...show less
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Odds in Favor of 'Pink Swan' Events
The following commentary comes from an independent investor or market observer as part of TheStreet's guest contributor program, which is separate from the company's news coverage.
NEW YORK ( TheStreet) -- A "black swan" event -- a rare, unexpected event that has a major impact -- is most often referred to as something with negative consequences. The near-record low investor confidence readings and double-digit decline in the stock market during the third quarter reflect concern that the odds have sharply increased that a black swan event may take place.
It is easy to cite a few of them: a European financial crisis; a U.S. recession; a fiscal debacle in Washington. However, investors have just as sharply discounted the odds of a positive surprise, or "pink swan" event. Given the pessimistic tone of investors, the pink swan may have the bigger potential market impact.
Last week the U.S. economic data came in better than expected and progress was made on the European debt problem with Germany's ratification of the expansion of the European Financial Stability Facility. However, this provided little relief to investors as stocks were basically unchanged on the week and remained near the low end of the 1120 to 1220 range the S&P 500 Index has been stuck in for the past two months. While investors fret over black swans, there are a number of potential pink swan events that could take place and grab investors' attention in the weeks ahead:
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Today I watched the last episode of Season 5 Charmed. I'm going to buy season 6 this weekend. I really liked the ending. It was new and different. I don't like that Piper and Leo kinda broke up and they have a new white lighter now. It will be exciting to see what happens next in Season 6.
How I Met Your Mother returns March 17. I can't wait to see new episodes. Only 12 more days of waiting. Samantha Who? returns on April 7. Only 32 or 33 days left until new episodes start. Moonlight returns April 25 and only 51 days left of waiting.
Joss Whedon's new show Dollhouse is coming out this fall. I can't wait to see it. 7 episodes were ordered from what I hear. I liked Buffy the Vampire Slayer, and Angel, so I'm sure I'm going to like Dollhouse.


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2015 BMW S1000RR in Black Storm Metallic
This 2015 BMW S1000RR in Black Storm Metallic is available for sale at Euro Cycles of Tampa Bay located at 8509 Gunn Highway in Odessa, Florida 33556 PH: 813-926-9937 WEB: www.EuroCyclesOfTampaBay.com
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Euro Cycles is proud to announce we have a new partner for our track days, Ron Croft, P.A. With Ron's sponsorship we will be able to do a lot more with track days, starting with our February 23rd date.
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New Delivery! This 2015 BMW Motorrad USA S1000RR in Black Storm Metallic
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