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Polymerase Chain Reaction
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List of recent Polymerase Chain Reaction-related patents
Control of chemical reactions using isotachophoresis
Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.
Method and kit for protozoa characterization
Disclosed are compositions, kits, and methods for detecting, extracting, visualizing, characterizing, and/or identifying one or more protozoa. Various types of polymerase chain reaction techniques in connection with specifically designed oligonucleotide probes can be used to detect a variety of, e.g., pathogenic, protozoa in samples..
Modification of sample preparation to differentiate live and dead bacteria by polymerase chain reaction assay
The invention relates to a method of determining whether a live microbe, such as bacteria, is present in a test sample.. .
Cutoff point delta ct. method for her2 pcr testing in breast cancer
The present invention is related to an improved method for her2 gene test by using quantitative real-time pcr (polymerase chain reaction) technique. Our invention streamlines test process, and incorporates quality control for each major step, including sample, reagent, operation, and data report.
Simplified gating method for sealing and flow control in micro and nano devices
A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
Method for assembling multiple target loci to single nucleic acid sequence
A method for assembling multiple target loci to a single assembled nucleic acid sequence involves assembling the multiple target loci to a single assembled nucleic acid sequence through two polymerase chain reactions (pcrs). A pair of primers for a primary amplification include a target-specific sequence and a 5′-flanking assembly spacer sequence.
Method for high-throughput screening of transgenic plants
The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (qpcr or real-time pcr), wherein the real-time pcr is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.. .
Error correction in nucleic acid molecules
An enzymatic method for removing sequence errors in nucleic acid molecules are described. The method utilizes a cel endonuclease that cuts heteroduplexes at mismatch sites containing the errors and an overlap extension polymerase chain reaction to re-assemble the cleaved fragments into full-length nucleic acid molecules free of the errors..
Development of novel detergents for use in pcr systems
This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (pcr). Methods for preparing the modified detergents are also described..
Fast pcr heating
Provided herein is a microplate for polymerase chain reaction (pcr), comprising a substrate formed of a material that is susceptible to heating pcr samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a pcr ramp rate of at least 5° c./second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate..
Compositions, methods and systems for polymerase chain reaction assays
The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddpcr) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddpcr assays using intercalating dyes.
Cartridge interface module
A cartridge interface module (cim), configured to engage with a removable microfluidic cartridge in a nucleic acid analyzer system can include a fluidics component, which is configured to initiate and support a liquid extraction of nucleic acids from a biological sample contained in the removable microfluidic cartridge. The cim also includes a polymerase chain reaction (pcr) assembly component which can be configured to initiate and support amplification of the extracted nucleic acids.
Detection method of micro-rna with high specificity
The invention provides a method for detecting mirna based on polymerase chain reaction comprising evagreen dye, and the use of evagreen dye in elevating the binding specificity of primers to templates in pcr.. .
Method for detecting and quantifying wheat endogenous gene
Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having at partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
Microfluidics polymerase chain reaction and high resolution melt detection
The present invention relates to a method and system for polymerase chain reaction ("pcr"), high resolution melt ("hrm") analysis and microfluidics, and, more specifically, to a method and system for implementing the processes of pcr and hrm on a microscale in a microfluidics chamber for certain purposes including for purposes of dna detection and/or extraction.. .
Method for detecting and quantifying wheat endogenous gene
Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
Tactical and portable pcr/hrm genome identification system
The present invention relates to a polymerase chain reaction and high resolution melt genetic identification system, and, more specifically, to a tactical and portable polymerase chain reaction and high resolution melt genetic analysis and identification system that is configured to determine and communicate analysis and identification results and a tiered confidence/alert level related to the analysis and identification results.. .
Methods and devices for non-therman polymerase chain reaction
Methods and devices for non-thermal pcr amplification of nucleic acid sequences. An electrical potential is applied to cause non-thermal separation of strands of a double-stranded nucleic acid or double-stranded nucleic acid/primer extension product..
Diagnostic microrna profiling in cutaneous t-cell lymphoma (ctcl)
The present invention relates to the field of cancer-diagnostics. In particular the invention relates to a microrna expression signature that allows discriminating skin samples of cutaneous t-cell lymphomas (ctcl) from non-malignant (inflammantory) skin samples by use of quantitative polymerase chain reaction performed on reverse transcribed mirna.
Microfluidic flow monitoring
The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system..
Molecular targets and methods for formulation screening and preservative efficacy testing
Methods and kits are disclosed for distinguishing viable from nonviable microbial cells. The methods and kits are useful in the screening of cell culture formulations and the testing of preservative efficacy.
Methods, devices, and systems for processing multiple assays background
Methods, devices, and systems for performing polymerase chain reaction (pcr) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both pcr temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the pcr temperature cycling for real-time pcr and/or during temperature ramping in the melt data acquisition.
Discovery of a somatic mutation in myd88 gene in lymphoplasmacytic lymphoma
Diagnostic assays for facilitating the diagnosis of lymphoplasmacytic lymphoma (lpl) are provided. The method comprises assessing a biological sample of the subject for the presence of a mutation at position 38182641 in chromosome 3p22.2, wherein presence of the mutation is indicative that the subject has lpl.
Apparatus and method for electrical detection of oligonucleotides through pore blockades
Systems and methods for specific nucleic acid (na) sequence detection that do not rely on polymerase chain reaction (pcr) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.. .
Telomere length measurement in formalin-fixed, paraffin embedded (ffpe) samples by quantitative pcr
Methods of reliably quantifying telomere length in cells or tissues that have been formalin fixed and paraffin embedded (ffpe) samples by quantitative polymerase chain reaction protocol and kits for use with such various methods are provided. The methods of the present invention may be used to predetermine an individual's response to treatment with a telomerase inhibitor, a telomere damaging agent or a telomerase activator..
Automatic detection kit for detecting hla alleles using real-time polymerase chain reaction
Provided is an automatic detection kit for automatically detecting hla alleles using a real-time polymerase chain reaction (pcr). The real-time pcr is performed on dna isolated from a sample using a primer which is able to specifically bind to hla alleles and a fluorescent probe which is able to detect amplification of the hla alleles in real time, and the hla allele typing is performed by analyzing a fluorescence value obtained from the real-time pcr using an hla automatic typing..
Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a single reaction method to co-amplify multiple subsequences of a nucleic acid fragment sequence (i.e., for example, at least two read pairs from a single library insert sequence).
Methods and compositions for preparation of nucleic acids
A method for isolating genomic dna (gdna) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gdna with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gdna; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the ph of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gdna is produced.
Methods and systems for visualizing and evaluating data
A computer-implemented method of generating a digital polymerase chain reaction (dpcr) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period.
Detection and quantification of biomolecules using mass spectrometry
The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry.
Dna fragment detection method, dna fragment detection kit and the use thereof
The disclosure claims a cleaved deoxyribonucleic acid (dna) detection method, a dna fragment detection kit and use thereof. Wherein, the method includes the steps of: designing primers according to a test site or a test region of the dna fragment; cyclizing the dna fragment to obtain acyclized dna; implementing polymerase chain reaction (pcr) amplification for the cyclized dna by using the primers; and detecting the pcr amplification product.
Method and kit for constructing plasma dna sequencing library
The disclosure relates a method and a kit for constructing a plasma deoxyribonucleic acid (dna) sequencing library. The method provided by the disclosure includes: extracting a plasma dna; making the plasma dna ligate to a sequencing linker, and purifying a ligation product; performing polymerase chain reaction (pcr) amplification for the purified ligation product, purifying the pcr amplification product, and obtaining the plasma dna sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma dna.
High throughput paired-end sequencing of large-insert clone libraries
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that improves the yield of long-distance genomic read pairs by constructing long-insert clone libraries (i.e., for example, a fosill library or a foscn library) and converting the long-insert clone library using inverse polymerase chain reaction amplification or shearing and recircularization of shortened fragments into a library of co-ligated clone-insert ends.
Methods for preparing cdna from low quantities of cells
Methods for preparing cdna libraries from single and low quantities of cells are disclosed. The methods are based on the principles of multi-strand displacement amplification or semi-random primed polymerase chain reaction.
Composition and methods for rt-pcr comprising an anionic polymer
The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an rna template and further nucleic acid replication.
Methods and compositions for detecting cancers associated with methylation of hmlh1 promoter dna
Methods are provided for detection of cancers associated with methylation of hmlh1 promoter dna in a subject. The method comprise assaying for the presence of methylated hmlh1 promoter dna in a bodily fluid from a subject.
Functionally integrated device for multiplex genetic identification
A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
Assay and other reactions involving droplets
The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
Assays and other reactions involving droplets
The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
Methods for determining cell viability using molecular nucleic acid-based techniques
The present invention relates to novel methods, and kits, for selectively excluding dead cells from a mixture containing live and dead cells, such as microbe cells in clinical samples, blood products, medical/biotechnology products and food products where subsequent interrogation of the selected live cells are an indicator of the presence of microbe viability. In particular, the invention relates to improved methods for performing direct nucleic acid amplification techniques such as polymerase chain reaction (pcr) and isothermal techniques in blood and other body fluids, for correlation with microbe cell viability from bacteremia and fungemia samples.
Method of preparing a reaction mixture and related products
The invention relates to a method of preparing a reaction mixture for polymerase chain reaction (pcr) assay and a solution set for pcr. The method comprises providing a sample solution comprising a biological sample to be amplified in said pcr assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the pcr process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors.
Gene analysis method using sdl-pcr
According to the sdl-pcr method of the present invention, non-specific amplification can be minimized by removing non-ligated probes or genomic dna using a tag, and separation can be achieved within a shorter time compared to a separation method that is performed using exonuclease. In addition, ligation, separation and polymerase chain reaction processes can be performed in a single solution in a single tube, and thus a plurality of genes can be amplified at the same time in an accurate and rapid manner..
Low-mass sample block with rapid response to temperature change
A sample block for use in the polymerase chain reaction, dna sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells.. .
Preparation of gene-specific templates for the use in single primer amplification
This disclosure relates to methods for creating engineered templates that are useful for amplification of one or more antibody genes without the use of gene-specific primers. More specifically, templates engineered using these methods in a polymerase chain reaction setting which allows for the specific amplification of one or more antibody genes..
Dry composition of reaction compounds with stabilized polymerase
The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (pcr) amplification after re-solubilization..
Methods for polymerase chain reaction copy number variation assays
This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference).
Detection kit for identifying genotype in depression patients and method of using the same
The present invention relates to a rs6311 test kit, which includes a probe, a primer, and a polymerase chain reaction solution, wherein said probe sequence is as follows: rs6311t-fam: ctgtgagtgtctggc (seq. Id.
Methods to predict breast cancer outcome
The present invention provides methods for predicting the outcome of a subject having breast cancer. The prediction model is based on the gene expression profile of the genes listed in table 1a or 1b.
Quantitation of human genomic and mitochondrial dna
Methods are provided for determining, in a single polymerase chain reaction (pcr) reaction, the quantity, quality, and gender of origin of dna in a sample, and whether or not the sample contains pcr amplification inhibitors. The methods involve carrying out a single pcr multiplex reaction utilizing primer sets specific for amplifying: the human amelogenin locus; an x- and/or y-chromosome specific gene that is shorter than the amelogenin gene; at least one mitochondrial dna sequence, and preferably two differently-sized mitochondrial sequences; and a heterologous, non-human reporter gene..
Single tube quantitative polymerase chain reaction (pcr)
Provided herein are systems and methods for quantitatively monitoring target amplicons produced by polymerase chain reaction (pcr). In particular, quantitative monitoring of target amplicon(s) in a single-tube pcr reaction without separate calibration reactions are provided..
Detergent free polymerases
The present invention relates to a formulation of a thermostable dna polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (pcr). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step..
Polymerization of nucleic acids using proteins having low isoelectric points
This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (pcr).. .
Inhibition-resistant polymerases
Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in polymerase chain reactions (pcr). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity.
Novel compositions, methods and kits for real time polymerase chain reaction (pcr)
The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.. .
Field-based qpcr microbial monitoring
Dna/rna monitoring of microbes at an oil field to determine the presence and activity of harmful microbes is accomplished with a portable qpcr (quantitative polymerase chain reaction) machine. This permits the monitoring to occur on-site in the field and reduces the variability that may occur from the transportation of samples..
Gene, ars-r anchorage cassette, ars-r expression-anchorage cassette, recombinant plasmid, bacterial transgenic lineage, use of said gene, use of said lineage in environmental bioremediation processes
The present invention relates to the construction and insertion of a dna plasmid vector of broad spectrum for gram-negative bacteria, that carries a gene sequence which, when expressed, enables the anchorage of a chelator protein for arsenic ions on the gram-negative bacteria cellular surface. For that end, the structural sequence of the regulatory arsr gene without stop codon (seq id no 1) was amplified by polymerase chain reaction (pcr) using as a template the chromosome 1 of cupriavidus metallidurans, ch34 lineage and inserted into the pgem-t cloning vector, yielding the pgemt-as plasmid (seq id no 2).
Method of mutation detection in blood cell-free dna using primer extension (pe) and pcr
A method of detecting mutation in blood cell-free dna, includes providing a serum sample, isolating dna from the serum sample, amplifying the dna by polymerase chain reaction (pcr), subjecting the pcr product to primer extension (pe), and separating the pe reaction product and identifying the mutation by gel electrophoresis. In order to improve accuracy and sensitivity, the pe reaction can be carried out by using a primer that blocks the extension of the wild or non-mutated sequence..
Thermally controlled chamber with optical access for high-performance pcr
Novel methods and systems for polymerase chain reaction (pcr) are disclosed. A pcr device has a bottom heater layer, a central reacting layer, and a top heater layer.
Mouse cell line authentication
A multiplex polymerase chain reaction assay that targets nine tetranucleotide short tandem repeat (str) markers in the mouse genome. Unique profiles were obtained from seventy-two mouse samples that were used to determine the allele distribution for each str marker.
Polymerase chain reaction
A polymerase chain reaction (pcr) device for a reverse transcriptase reaction (rt) and a convectively-driven polymerase chain reaction (cpcr) in the same device is provided. The pcr device comprises an upper temperature-controlling unit, a middle temperature-controlling unit and a lower temperature-controlling unit.
Varietal counting of nucleic acids for obtaining genomic copy number information
A method for obtaining from genomic material genomic copy number information unaffected by amplification distortion, comprising obtaining segments of the genomic material, tagging the segments with substantially unique tags to generate tagged nucleic acid molecules, such that each tagged nucleic acid molecule comprises one segment of the genomic material and a tag, subjecting the tagged nucleic acid molecules to polymerase chain reaction (pcr) amplification, generating tag associated sequence reads by sequencing the product of the pcr reaction, assigning each tagged nucleic acid molecule to a location on a genome associated with the genomic material by mapping the subsequence of each tag associated sequence read corresponding to a segment of the genomic material to a location on the genome, and counting the number of tagged nucleic acid molecules assigned to the same location on the genome having a different tag, thereby obtaining genomic copy number information unaffected by amplification distortion.. .
Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
A method is provided for reverse transcription-polymerase chain reaction (rt-pcr) comprising a) amplifying a reverse transcribed cdna in a mixture comprising norovirus genogroup i and norovirus genogroup ii primers and probes, wherein said norovirus primers and probes distinguish between genogroup i and genogroup ii viruses; b) quantifying virus; and c) normalizing data based on a universal internal rna control. Optionally, the method may also include primers and probes for enteroviruses.
Human exhaled aerosol droplet biomarker system and method
A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.. .
Modified nucleosides for the treatment of viral infections and abnormal cellular proliferation
This invention also provides an effective process to quantify the viral load, and in particular bvdv, hcv or west nile virus load, in a host, using real-time polymerase chain reaction ("rt-pcr"). Additionally, the invention discloses probe molecules that can fluoresce proportionally to the amount of virus present in a sample..
Devices and methods for biological sample-to-answer and analysis
Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk.
Methods of fabrication of cartridges for biological analysis
Methods to fabricate reaction cartridges for biological sample preparation and analysis are disclosed. A cartridge may have a reaction chamber and openings to allow fluids to enter the chamber.
Micro-fluidic modules on a chip for diagnostic applications
A chip having a substrate for amplifying genetic material includes a substrate heater having a plurality of substrate heating resistors. The heating resistors define a first temperature zone for maintaining a first temperature, a second temperature zone for maintaining a second temperature below the first temperature, and a third temperature zone for maintaining a third temperature between the first and second temperatures, on the substrate.
Thermal cycler apparatus and related methods
An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells.
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What does FMF mean in Military?
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Foreign Military Financing
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Ever play the cinematic trope drinking game? That's where you take a shot every time a Hollywood flick uses blunt editing to make a point,... More >>
In a perfect world, Disney on Ice would be some nefarious mobster plot to silence stupid Ariel and her anti-female-empowerment nonsense by sending... More >>
Classical ballet can look pretty stern from the audience's perspective. It's all tightly pinned hair, feet that are ramrodded into... More >>
Broadway brings us many wonders: soaring story lines, uplifting anthems, dazzling sets. It's also chock full of annoying little... More >>
Hollywood's awards season is over, and instead of being stuck watching an already self-obsessed industry lavish honors upon itself, we can... More >>
Shakespeare starts getting pounded into your head somewhere early in your high school experience, so much so that you begin to take it for... More >>
Casablanca was an ordinary film by 1940s Hollywood standards — it wasn't made to be a blockbuster, nor was it expected... More >>
You tried so hard to keep those damn princesses out of your house: blanket Disney bans, female-empowerment board books, pretend lab coats instead... More >>
If you had to do a thesis for graduation, you probably spent months and months on minutiae — manipulating data, shedding tears and mining... More >>
The true gift of the arts is that they inherently transcend language, even culture, and speak to each viewer or listener as though they share the... More >>
There's a passage in J.M. Barrie's Peter Pan that says something about how stars are really only observers, that they... More >>
It's true: There are so, so many fish in the sea. But you've managed to get one on the line — a real keeper, it seems —... More >>
Avoid the restaurants like the plague on Valentine's Day. Since the day itself falls on a Friday this year, it'll be an unholy... More >>
A day trip to Denton is in order. You haven't really been back to appreciate all it has to offer in a while — you've got fond... More >>
The silly time of year when love goes to our heads is upon us. We exit football season, wipe the wing sauce off our mouths and then ante up for... More >>
Nobody knows how to do camp anymore. Humor in modern film and TV is so wrapped up in irony that not a sliver of campiness can wedge in among all... More >>
Your Instagram account is chock-full of perfectly framed eye-candy, prompting mass "like"-age and plenty of comments about your... More >>
If you've never seen an installment of Naked Girls Reading, you might have some preconceptions about what exactly is involved.... More >>
You can't make this stuff up ... said no fiction writer, ever. Mostly because they are pretty sure they can. But despite the twisty plot... More >>
We're at that dead part of the season where it's not consistently sweater weather, but we can't quite get away with spring stuff... More >>
When you look at the cultural fabric of Dallas, you see a fair amount of artistic trailblazing: the DMA, for example, taking museum/community... More >>
Oh, but who doesn't love a parade? Especially the kind that brings a city together, lining streets from downtown to Fair Park with cheering... More >>
If you've ever accidentally oared a buddy in the face, or rowed enthusiastically in circles until you figured out that coordinating your... More >>
David Garrett looks like he should be emblazoned across one of your mom's old romance novels, edging Fabio out with the dull end of a... More >>
Buddy movies are so very "been there, done that," right? Old cop, young cop. High-ranking cop, goofy patrol cop. You know the drill.... More >>


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There are 18 results - Show results on a map Print this page
Showing 11 - 18 of 18
Hazelmere is peacefully located overlooking the River Greta and perfect for walking in the surrounding fells or to the shores of Derwentwater. The market place is only a short stroll away. The house h...More about Hazelmere
The Royal Oak, in the pretty village of Braithwaite, has delightful views of Skiddaw and Barrow, and is a good base for tourists and walkers. Some of the well-equipped bedrooms are furnished with four...More about The Royal Oak
Close to both Keswick and Bassenthwaite, set in rolling countryside, this small country house hotel offers warm, genuine hospitality along with high quality food. Bedrooms are well equipped and appoin...More about Ravenstone Lodge
Ideally located on the edge of Keswick town centre, this ground floor accommodation was converted from what was the old Storms Farm Dairy known locally as the "lemonade factory". En-suite bedrooms are...More about Brocklehurst B&B
This impressive Victorian hotel is set amid attractive gardens close to the town centre. Eight superior bedrooms are available in the Station Wing, which is accessed through a conservatory. The attrac...More about Keswick Country House Hotel
A friendly welcome waits at this hotel which occupies a prime position in the centre of the historic market town, and is a popular meeting place for the local community. Bedrooms are mostly well propo...More about The Golf Hotel
This charming property sits on wooded hills with striking views of Skiddaw, and is conveniently placed for Keswick. The owners provide excellent hospitality in a relaxed manner. The award-winning food...More about The Cottage in the Wood
This hotel is modern in style and offers thoughtfully equipped accommodation. It is well situated for the Northern Lakes area and has good road links. The restaurant, open all day, serves a wide varie...More about Shepherds Hotel


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Encyclopedias Online Library Resources
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-- World Book Encyclopedia's excellent editorial content, rich media, and interactive features in Spanish.More Information »
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-- Grolier Online includes the following resources: Grolier Multimedia Encyclopedia, The New Book of Knowledge, La Nueva Enciclopedia Cumbre, America the Beautiful, Lands and Peoples. Encyclopedia information on a wide range of topics is available by searching and browsing through these databases. Please note: The library's subscription to Grolier Online expires November 9. For a similar resource, please use World Book.
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Hints about shtf knives
View Single Post
10-01-2012, 16:23
Senior Member
Join Date: Aug 1999
Location: Arkansas, USA
Posts: 8,153
Originally Posted by
3. Understand that a knife is a tool. No one tool is versatile to handle all demands.
7. Obtain a decent sharpening system and know how to use it. Heck, I went to college. But I cannot maintain a 30 or 40 degree angle on a free sharpening stone. However, I can move a knife up and down vertically on stones that are properly angled, so I chose the Spyderco system.
12. Think about whether you want to have a folder and/or leatherman in each knock about jacket, pack.
Well said.
Theres nothing in your post that Id disagree with, but these are so crucial as to be worth repeating over & over. Or maybe theyre just my personal hobby-horse soapbox points.
No one single tool is the answer. About the time we decide on the perfect crowbar, we need a scalpel.
On sharpening, my sharpening weakness is a plain old stone. I can get a knife sharp with one, but I cant get a knife
with one,
as fast as I should be able to
. Give me my eze-lap and Im hitting extremely-sharp in no time. Give me a mousepad and some sandpapers down to 6000 grit, and were hitting oy-vey-sharp pretty quick. But a flat stone, it takes me longer than it should; a failing of mine, but a minor failing as I keep eze-laps all over the place. (One of those a mans got to know his limitations kind of thing.)
Major +1 also on just having pocket knives or multitools in jacket pockets, glove compartments, briefcase pockets, etc. Like a flashlight or lighter, its one of those things that its hard to genuinely have too many distributed around.
"The best a man can hope for is a chance to prove that the good lord didn't make a mistake putting him here in the first place." - Will Sonnett
"Only problem with women my own age, is they're so damn old." - my dad at 89...
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3D Systems Corp (NYSE:DDD)
The Company through subsidiaries designs, develops, manufactures, markets and services rapid 3-D printing, prototyping and manufacturing systems and related products and materials.
Graham number = 22.5 * 0.68 * 7.3 = 111.69 ?
Your Graham number, is this good or bad? My look at the numbers gives an slight edge to ddd over syss. NASA uses the 3D printers, so you would think they want the best.


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A.W.O.L. Rescue
Dog adoption saves lives. Adopt a dog and you'll have a friend for life! Contact us, or contact another local humane society, animal shelter or SPCA.
(214) 755-1662
Pets at Our Rescue Group:
Cities, Towns, and/or Counties We Serve:We are a non-profit organization located in the suburbs of Dallas, Texas. The dogs/puppies we have are all rescued from various shelters or clinics. Some are mixed breeds, some are full blooded, but all have American Pit Bull Terrier.
About Our Rescue Group:We are a private non-profit organization located in the suburbs of Dallas, Texas. WE ARE NOT A TYPICAL SHELTER with walk-in accommodations. Please email firstname.lastname@example.com. for an adoption application. Once your application is approved, we will set up a convenient time for you to meet our dogs. The dogs/puppies we have are all rescued from various shelters or clinics who were on death row.
Come Meet our Pets:We are a private rescue and show our dogs/puppies by appointment only.
Our Adoption Process:We have an adoption application which must be completed and approved before our 'rescues' are met and the adoptions are finalized. Our adoption fee varies depending on the vet care needed on individual dogs/puppies starting $250.00. You may email our rescue for an application.
If you would like to help us in saving more lives of this wonderful and loving breed, please click on the donate icon below. Thank you in advance for your kindness and help.


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Light Blonde is our second lightest shade with multi-tonal golden hues. The delicate colour, with it's golden hues, is the epitome of sun-kissed beauty. The multi dimensional highlights and lowlights of the hair help it blend seamlessly with those cooler tones.
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Raging Moderate, by Will Durst
Let me be among the first 40-foot, helium-filled balloons to kick-start our national parade of giving thanks. That's right, we're just about to butt heads with Turkey Holocaust Day, and to be perfectly honest, it's about time. A little tryptophan poisoning might be the perfect prescription for these trying times.
Doubly comforting because this particular holiday isn't about greasing the wheels of capitalism with the fire-hose of consumer debt like that other holiday about a month down the road, which shall remain nameless. And this one doesn't hide under any religious robes either. It's purely about the journey to Comfort City through the Gluttonous Woods. Food, family, friends and football. Four of the five F's.
So, allow me to express my gratitude for the fourth Thursday of November. One of the little things that go a long, long way to making life worth living. And here are a couple other examples of what a middle-aged, round-headed political pundit bows his head and gives thanks for.
Barack Obama. Because no matter what you think of his policies, you got to admire his ability not to get involved in them.
Dick Cheney. Six heart attacks and the man still manages to go on a book tour. How does a guy without a heart have six heart attacks? It would be like Rick Perry contracting a brain tumor.
Rick Perry suffered a 53-second brain freeze during a national debate. Fifty-three seconds. It only took the San Francisco 49ers eight seconds longer to score two touchdowns last Sunday. The Niners!
Former Democratic New York Congressman Anthony Weiner, who escaped the press by entering sexual rehab. "I'm a sexual addict." Yeah. There's another name for that. We call it — Male. The man is simply suffering from a not-so-atypical case of Y chromosome poisoning.
Newt Gingrich for refusing to go gently into that good night. Even Brett Favre is saying, "Give it up, old man."
Herman Cain, whose long-form, cross-country Fox News audition has exceeded all expectations. Roger Ailes must be so proud.
The Occupy Wall Streeters. The "1%" dismiss the Occupiers due to questionable hygiene. Just because you smell odd doesn't mean your message is any less true. The fact they can't afford Chanel No. 5 may be part of the point.
Bill Clinton, who refuses to go away. God bless him. Although, President Obama might harbor another opinion.
Michele Bachmann. Her Newsweek cover photo made her look spooky, so supporters complained they cherry-picked a creepy-looking photo on purpose. Then the magazine put the entire photo shoot up online, asking, "Which one would you have picked?" And everybody shut up.
The entire Democratic Party, for failing to realize they're in the middle of a war. Republicans attack them with torches and pitchforks and the Democratic response is to introduce legislation to reform pitchfork safety standards.
The entire GOP, which is waging an internal war for its very soul. The GOP Soul. Short book. Put it on the shelf right next to Great Democratic Leadership Battles.
Sarah Palin. Who refuses to go away. God bless her. Although, Mitt Romney might harbor another opinion. Or two. Diametrically opposed to each other.
Pat Robertson, who called the Republican presidential field too extreme. Pat Robertson blasting his party for extremism. That's like having your drug intervention hosted by Lindsay Lohan. And Charley Sheen is driving the van.
You can't make stuff up like this. See, I'm telling you. Life is good. Thankfully yours.
The New York Times says Emmy-nominated comedian and writer Will Durst "is quite possibly the best political satirist working in the country today." Check out the website: willdurst.com to find out more about upcoming stand-up performances or to buy his book, "The-All American Sport of Bipartisan Bashing."
Catch Durst back in Wisconsin for his annual Turkey Trot on Wednesday the 23rd and Sunday the 27th at the Safe House, 779 N. Front St., Milwaukee. safe-house.com. 414.271.2007. Friday the 25th at Paolo's Pizza. 5121 W. Howard St, Milwaukee. Wisconsin. 414.727.9332; and Saturday the 26th at the Railroad Station. 200 S. Railroad St., Saukville. 262.268.7880.
Copyright ©2011, Will Durst, distributed by the Cagle Cartoons Inc. syndicate. Call Cari Dawson-Bartley at 800-696-7561 or e-mail email@example.com. Will Durst is a political comedian who has performed around the world. He is a familiar pundit on television and radio. E-mail Will at firstname.lastname@example.com. Check out willandwillie.com for the latest podcast. Will Durst's book, "The All American Sport of Bipartisan Bashing," is available from Amazon and better bookstores all over this great land of ours. Don't forget to check out his rooftop comedy minutes at: http://www.rooftopcomedy.com/shows/BurstOfDurst.


text:
Member Locator
The APP name and logo are trademarks of the Association of Professional Piercers. Unless otherwise stated, content on this site is licensed under the
Creative Commons Attribution-Noncommercial-No Derivative Works License.
APP Procedure Manual
APP Conference Program
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The Point Journal
Informative Brochures
Brochures in English, French and Spanish! Download all 8 APP Brochures


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|New Living Translation (NLT)
|New International Version (NIV)
|3 When the watchman sees the enemy coming, he blows the alarm to warn the people.
|3 and he sees the sword coming against the land and blows the trumpet to warn the people,
|Scripture quotations marked NLT are taken from the Holy Bible, New Living Translation, copyright 1996. Used by permission of Tyndale House Publishers, Inc., Wheaton, Illinois 60189. All rights reserved. (New Living Translation - The Bible Online)
|Scripture quoted by permission. Quotations designated (NIV) are from THE HOLY BIBLE: NEW INTERNATIONAL VERSION®. NIV®. Copyright © 1973, 1978, 1984 by Biblica. All rights reserved worldwide. (New International Version Bible Online)


text:
>> Size Chart
* Please use the sizing chart to ensure you get
the correct size when you place the order.
You are welcome to exchange your item. Exchanges are allowed one time only.
However, please keep in mind there is a 15% restocking fee as well as shipping
Side Ribbon Organza Dress w/Satin Top
Flower Metallic Applique Taffeta Dress
Rose Swirl Print Organza Dress w/ Satin Bodice
Sequince Embroidred Mesh Top w/Pleated Organza Waistband Flower Girl Dress
Bouquet Embroidered Organza Dress


text:
1) Sikorsky Financial Credit Union
1000 Oronoque Lane
(203) 377-2252
other locations
Voters Said:
The best - no matter what location, the experience is always positive.
Excellent service.
Great personal service, considering that other large financial institutions have lost it.
2) Mutual Security Credit Union
6580 Main St.
(800) 761-2400 x2800
3) Connex Credit Union


text:
Recent Forum Posts
View More
Recent Comments
- 4 months ago i would like to win one, looks like a decent phone evening if that can't be said about the company / brand.
- 1 year ago I want this!
- 1 year ago Choose me please!
- Member for
- 1 year 9 months


text:
B/grades 2-3
Myseum, 283 Lamp and Lantern Village, Town and Country, 63017
What is DNA? What does it look like? You will see strawberry DNA and wear it home on a special necklace. After the program you will be free to wander through the Myseum building and learn much more.
$10 per girl
Deadline: Dec. 27
Contact: Lauren Commerford, 314.592.2359, email@example.com
Girl Scouts of Eastern Missouri is a proud United Way member and is supported by the following United Way Agencies: United Way of Greater St. Louis, United Way of the Mark Twain Area, United Way of Adair County/Northeast Missouri, United Way of St. Francois County, Franklin County Area United Way.


text:
Dark Comedy The Waiting Room at City Lights
* Additional fees apply.
The last date listed for The Waiting Room was Friday February 25, 2005 / 8:00pm.
Most Popular Theater Event Nearby
Broadway San Jose: 3-Show Package for Hit Shows
- Full Price:
- $189.00
- Our Price:
- $174.00
You don't have to travel to New York to catch the hottest Broadway shows when you get this three-...Learn More
Venue Details
Make sure you print out your ticket confirmation, so you will be waved through. Otherwise , you will need to stop and have your purchase confirmed. Although I understand the need to "reserve" seats for the lobby volunteers in this general seating theatre, choosing less conspicuous seats would be a better option.Monty Python's Spamalot info • Aug 11 2014 star this tip starred
$5 parking across the street from the theaterAmadeus info • Apr 28 2014 star this tip starred
Quotes & Highlights
American Theatre Critics Association Award Winner
!"A bold, risky melange … terrifically moving!" —New York Times
A satirical and controversial examination of the timeless quest for beauty – and its costs. An 18th century Chinese woman, a tightly-corseted Victorian lady, and a much-augmented Jersey gal meet in a modern day doctor's office, each confronting the forces that led them there. This always-lively dark comedy is a wild ride through medical and sexual politics with a good dose of resilience and humor.
Written by Lisa Loomer
Directed by Kit Wilder and Lisa Mallette
Featuring Nichole Hamilton, Heidi Kobara, Lauri Smith, and D. Renee Willis with Larry Barrott, Dirk Leatherman, Wayne Lee, Derek McCaw and Ron Talbot.
About the Ticket Supplier: City Lights Theater Company
The City Lights Theater Company (CLTC) is San Jose's Off-Broadway theatre company and presents adventurous live stage productions in an intimate flexible environment. CLTC is committed to the community by providing their audiences with affordable, quality theatre and opportunities for local artists.


text:
Taco Lasagna Recipe
- 1 pound ground turkey or beef
- 1/2 cup chopped green pepper
- 1/2 cup chopped onion
- 2/3 cup water
- 1 envelope taco seasoning
- 1 can (15 ounces) black beans, rinsed and drained
- 1 can (14-1/2 ounces) Mexican diced tomatoes, undrained
- 6 flour tortillas (8 inches)
- 1 can (16 ounces) refried beans
- 3 cups (12 ounces) shredded Mexican cheese blend
- In a large skillet, cook the beef, green pepper and onion over medium heat until meat is no longer pink; drain. Add water and taco seasoning; bring to a boil. Reduce heat; simmer, uncovered, for 2 minutes. Stir in black beans and tomatoes. Simmer, uncovered, for 10 minutes.
- Place two tortillas in a greased 13-in. x 9-in. baking dish. Spread with half of the refried beans and beef mixture; sprinkle with 1 cup cheese. Repeat layers. Top with remaining tortillas and cheese.
- Cover and bake at 350° for 25-30 minutes or until heated through and cheese is melted. Yield: 9 servings.
Medium-Bodied Red Wine
Enjoy this recipe with a medium-bodied red wine such as Chianti, Sangiovese, Malbec or Zinfandel.
Reviews for Taco Lasagna
Sort By :
"it does make the outer edge of the casserole , bit crispy on the tortillas when bake for 30 minutes. But it is a great time saving recipe ,if you need dinner on the table fast. if you like southwestern food it would be a good recipe to try out, I just suggest baking it for 20 or 25 minutes any longer the outer edges of the tortilla will be to crispy."
"Wow! What can I say! I submitted this recipe 12 years ago on a whim and it won me $500.00 and a feature in Quick Cooking. I am so excited to see everyones' reviews and modifications. I am married now and my family loves this recipe too! Crazy to see 86 reviews written! Who knew!"
"This is delicious!!! I did add extra cheese (I love cheese!) I added chopped roma tomatoes, sliced black olives and sour cream to the top when serving. I think I will add chopped green onions the next time. Love this and my husband loved it!! I'm glad to see that it warms up good! Will definitely make again."
"Very good!! I served it with sour cream and that's tops it off to great! I changed the original recipe: ground turkey breast, canned diced tomatoes, canned green chiles, added Mexicorn, used La tortilla Factory tortillas, FF refried beans and 16 ounces (vs12) cheese. I baked @375º for 30 minutes covered, then uncovered and baked for another 30 minutes. The top got nice and crispy. So good and would serve 12."
"This was pretty good but not a favorite as far as "Mexican" lasagnas go. My family likes the other one I make better."


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Super Smash Bros. Brawl Answers for Nintendo WiiHome / Nintendo Wii /
Game Questions & Answers (Q&A) service lets you ask questions about video games for game consoles or PC games. So ask your Super Smash Bros. Brawl question for Nintendo Wii and get answers from real gamers or answer questions and share your insights and experience with the rest of the gaming community.
- Developer: Game Arts
- Publisher: Nintendo
- Genre: Action Fighting 3D
- Release Date: Mar 9, 2008
- ESRB: Teen
Question & Answers
Subspace emissary: maze stages
I'm stuck on the mazes and cannot find any new rooms and my map fills up half the screen when i bring it up. just need to know if there's any other rooms, cause i just keep going in circles ...
Answer from: emark20
Look for the dots. thats how i did it
Answer from: king dedede
The dots are doors that lead to more rooms check those.
Answer from: AAAgentX
Here's a hard room to find. I was stuck on the Great Maze for a month because I couldn't find it. In the Ruins, when you first enter the elevator, use a teleporting character to go on top of the elevator. Or one player could control the elevator while the other walks on to the top while it is going down. Either way, the elevator will come back up. there is a path to the right and a platform to the left. However, if you do not move quickly, the elevator will crush you and kill you. Hope this hint helped.
Answer from: boo vs dry bone
Dots = doors
bowser-like faces = bosses
Unanswered Questions
From: BlueDragon
Posted on: 11-12-2014
Monster High Skultimate Roller Maze - Official Launch Trailer
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PSA World Tour Squash - Gameplay Video
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The Penguins Of Madagascar - Gameplay Review Trailer
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- The Voice: I Want You, (Wii)
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- FIFA 15, (Wii)
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I use a biblical, solution focused approach to counseling with an emphasis on thought life, understanding identity in Christ and developing a biblical/eternal worldview. Time is spent exploring the client's value system and potential conflicts with their spiritual beliefs. Thought life is looked at extensively with focus on helping clients identify lies versus truth.
Christian counseling Cedar Rapids, IA is offered by the same licensed credentialed therapists, but with a focus on spirituality and Biblical guidance. Christian marriage counseling Cedar Rapids IA through Theravive will put your family and your relationship as a priority over separation and divorce. Our Cedar Rapids IA Christian counselors will help guide your life in Iowa through the lens of Christian principles so that you can navigate the right path ahead. Contact a Cedar Rapids IA Christian marriage therapist or Cedar Rapids, IA Christian counselor to begin your new journey of discovery and healing.
Welcome to our network of Cedar Rapids IA Christian counseling for marriages, families, relationships, and individuals. If you are seeking a Cedar Rapids Christian marriage counselor or Christian therapist, our professionals are able to help you. Emphasising Christian and Biblical truths, therapy with a licensed Christian counselor in Cedar Rapids, IA can help bring you through the issues you face, and restore your life to the right path.
You can move through the issues and find the new freedom that you hope for. We are here to help you make the first step. Make your journey the road you have longed to travel...don't be afraid to reach out, contact a therapist, and get beyond.
Theravive does not provide medical advice, diagnosis, or treatment. We provide information only. Theravive is intended as an informational resource of independent, private practicing clinics and counselors. We cannot guarantee the credentials or accuracy of any person or information listed on our site. Therapists on our site are not affiliated with Theravive. Please check and verify the credentials of any therapist you contact. Not all therapists listed are psychologists, and in any given city there may or may not be a psychologist listed. You are solely responsible for your use of information obtained on our site and are strongly advised to use due diligence before choosing a therapist.
Read here for more info.


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I make my special workouts a game with the kids. This way I have to do it and the kids get their workout as well. If I have to do my stand in place marching I get them to go against me. They have no clue I have to count my steps but, the goal is to go longer than me. And at 1 min I stop and they keep going. NOW they have won.
I spend more and more time worrying about the little things, when I should really look at what I have. Two great kids, who are my biggest motivators to eat better, do more and relax and play games with them. I keep finding myself wondering what I would do without them, and the answer is simple NOTHING! I keep going because my kids keep going.
I like exercising best when I am having fun- playing basketball, playing in the surf, running around after or with the kids.
If you have (active) children look at them when they play. They play hard and enjoy it. Try to forget to be an adult and play the way they do. They don't worry about burning calories or time. They just feel the wind in their face and the sun on their backs as they run, jump, bike or whatever. Laugh and play like a child. (You'll burn calories)


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June 9 (Bloomberg) -- Eskom Holdings Ltd. Chairman Mpho Makwana said he is "unaware" of his possible dismissal as leader of South Africa's power utility.
Makwana plans on serving his term, which is due to end in March 2013, unless he is informed otherwise, he said by phone today. Makwana was responding to a report in Business Day newspaper, which said South Africa's Cabinet approved the replacement of the chairmen of Eskom, ports and rail operator Transnet SOC Ltd. and defence contractor Denel Ltd. Eskom's entire board with the exception of Bernie Fanaroff will be replaced, the Johannesburg-based newspaper reported, without saying how it got the information.
To contact the reporter on this story: Franz Wild in Johannesburg at firstname.lastname@example.com
To contact the editor responsible for this story: Ana Monteiro at firstname.lastname@example.com


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Brick Builders LEGO Club
Calling all master builders ages 8-12! Join us for an informal LEGO program in the Main Reading Room at the Grafton Public Library on Wednesday January 21 from 3:30-5 pm. We will provide one model to replicate, one activity, and free build time. Space is limited, registration is required. Registration opens January 2, 2015.
Got an old set of LEGO bricks in the basement or attic? Drop off LEGO in good condition at the Library .We'll even sanitize them so you don't have to! Don't have old LEGOs, but still want to help? Feel free to purchase new sets or buckets of LEGO to donate, or purchase through our Amazon.com wish list at www.amazon.com/gp/registry/wishlist/15KP1LOVKYJDN
Donating to the Library is tax deductible (we'll provide proof, but cannot assess value).
For more details, to register, or to donate Legos, please contact the Library's Children's Room, at 508-839-4649 x4 or by email at firstname.lastname@example.com comments


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52 Sundays - June 15
This week focuses on Zach and Amy Weighrauch and their ministry in Cleveland Heights, Ohio.
Download the attached files below to use these resources in your church or small group.
You can also download a pdf copy of the full 52 Sundays from the main 52 Sundays article, 2014 prayer booklet or access an online version.184


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Barley Crusher or Malt Mill prefered. Let me know what you have! Thanks!
I have an original Phil Mill if you are interested.
I will have a barley crusher for sale soon, used 3x. Looks like santa is bringing me a monster mill! PM if interested.
Morebeer's deal of the day is this mill for $75
Keg #1: Sour Raisin Cider
Keg #2: Bonfire Imperial Smoked Amber Ale
Keg #3: Fairfield Nut Brown
Keg #4: Here Gose Nothing...
Keg #5: Empty
Bottle Conditioning: Mackenzie's Barleywine
In Primary: Nada
In Secondary: None
On Deck: KarmaCitra Pale & Berliner WeisseUntappd: absoluthamm


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Your Satisfaction is Guaranteed:
As he says in his preface, his book is for the person "who wants to engage in a new and different athletic experience without turning the rest of his or her life upside down in the process and wants to have fun doing so." Focusing on the "marathon-equivalent" triathlon, which is a 1.5-kilometer swim, a 40-kilometer bike, and a 10-kilometer run (although he does have a brief chapter called "Doing the Duathlon and Going Long"), Jonas helps you decide if a triathlon is right for you, gives his own personal history of how he went from a nonathlete to a racer, and advises on how to pick your first race.
From there, he discusses techniques, the basic principles of training, and how to establish your "aerobic base," the basic level of fitness (especially important for nonexercisers) you need before approaching his "Triathloning for Ordinary Mortals Training Program," a five-hour-a-week, 13-week program to train you for your race. Also covered in the manual is equipment and nutrition. Jonas's style of writing is accessible to the layperson--he doesn't burden you with technical terms or complicated zones or training levels. He even goes through an entire race with you, from the night before to putting air in your bike tires through the actual events right up to the aftermath of the race. The terrific appendix includes diagrams of stretches. While this isn't the book for a seasoned runner looking to improve his overall time, this is the ideal book to provide guidance and encouragement for newbies to the sport. Reading just a few chapters will have you itching to start racing. --Jenny Brown
Triathloning for Ordinary Mortals
Jonas, Steven; Aronson, Virginia
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Jamaica's Veronica Campbell-Brown fell short of the gold medal in the 200-meter dash in her first Summer Olympics since she won the event in Athens in 2004.
Ryan Brathwaite turned in the second-fastest qualifying time in the first round of the 110-meter hurdles on Tuesday at the Summer Olympics in London.
Former Barton Community College track and field athlete Tyson Gay finished fourth in the 100-meter dash at the 2012 London Olympics.
Veronica Campbell-Brown of Jamaica highlighted the performances of former Barton Community College athletes at the 2012 Summer Olympics in London during Friday's and Saturday's events.
After paying another hospital visit to Allen Trent in Wichita earlier in the week, Barton Community College head baseball coach Mike Warren says he's encouraged by his progression.
The laundry list of Olympic athletes with Barton Community College ties is long and impressive.
LONDON - Former Barton Community College track and field athlete Tabarie Henry has been granted a rare honor by his native country Virgin Islands.
The Barton Community College men's and women's tennis programs have added more area talent this offseason.
A Barton Community College baseball player remains in critical condition after a one-car traffic accident near Andale during the early-morning hours on Sunday, July 8.
Mitch Kottas, a projected outfielder/pitcher, officially signed to play baseball for Barton Community College on Wednesday morning at the Lawson-Biggs Field clubhouse.
Barton Community College had seven sophomore student-athletes receive national academic honors for the 2011-12 season.
With a 3.79 combined GPA, the Barton Community College women's tennis team earned the National Junior College Athletic Association Women's Tennis Academic Team of the Year.
PRATT - Great Bend native and Pratt Community College women's soccer player Kayci Huslig earned a 2011-12 NJCAA Academic Student-Athlete Award this year.
The registration deadline for the Barton Volleyball League is Monday, June 25.
The Barton Community College volleyball program is offering camps for middle school kids on Friday and high school individuals on Saturday.
It was a Homecoming of sorts for the Barton Community College men's basketball team – in Kalamazoo, Mich. that is.
The final visit to the Barton Gym for Coach Francis Flax was a memorable one. The Brown Mackie College men's basketball coach, wrapping up a 32-year career this season, made his final visit to a gym that he has visited probably about as many times as any other gym but his own over his career. In that final game Saturday afternoon the veteran coach left with a 97-93 win over the host Barton Community College Cougars.
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Contact InformationIpower Studios Llc
50 Inwood Rd
Darien, CT 06820
Other InformationEntity Status: Active
Type of Entity: Domestic Limited Liability Company
Entity Creation Date: Jul 08, 2010
Days in Business: 1627 days
Company Description
Ipower Studios Llc is located in Darien, Connecticut and is listed as an active Domestic Limited Liability Company. Ipower Studios Llc was started on Jul 08, 2010 which was 1627 days ago.
EmployeesAlexander Britton: Member
Registered AgentUnited States Corporation Agents Inc
Ct, CT NONE
Remove Profile
To remove the profile for Ipower Studios Llc, please click here.
Edit ProfileEdited: 12/1/2011
To edit this profile, please click here. You will be able to add a custom description, social networks, website, phone number, and many more features to your ISHCC.org profile.
Reviews0 Reviews
To add a review, please click here (you must be logged in to add a review).


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You must log in to access this feature. Sign in with Twellow or Facebook to get started.
By signing in to Twellow, you indicate your agreement to our terms of service.
Simply connect with Facebook and kiss those password blues away,
or go here to reset your password.
I love gym, animals, nature,Madonna, R'nB' and Hip Hop music, Reggae, Electronic and pop, and love weed! I'm gay and i wanna spend my life with my BF!


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- Hotel Details
- Member Reviews
- Photos
Riverfront Motel & Villas3 Stars
11 Strathaven Drive Rosetta Hobart TAS 7010
Riverfront Motel is located in Rosetta in Tasmania, overlooking the river and nine kilometres to Hobart centre. Nearby, vacationers can visit Moorilla Estate and the Museum of Old & New Art.
All 65 cosy rooms and self-contained villas and houses are individually fitted and come with modern amenities. Some units have full kitchen, living and dining areas, deck/patio, courtyard, barbecue facilities and laundry facilities.
At Riverfront Villas & Motel, patrons enjoy delicious menus all day at the in-house restaurant and bar or prepare meals in the self-catering villas and houses.
Riverfront Motel & Villas provides Wi-Fi access, laundry facilities and fax/photocopy facilities for an added charge. Vacationers can unwind in the outdoor solar heated lap pool, half court tennis and children's playground on site, sample chocolates at Cadbury Visitor Centre and explore Battery Point.
Hotel Facilities
- Business Centre
- Reception
- Concierge
- Restaurant
- Lobby
- Room Service
Room Facilities
- Air Conditioning
- Colour TV
- Alarm Clock
- Iron / Ironing Board
- Cable / Satellite TV
Other Recommended Hotels
|Quest Trinity House Hobart Hotel
|3.5 Stars
|Quest Waterfront Apartments Hobart
|4 Stars
|Rydges Hotel Hobart
|3 Stars
|Best Western Hobart
|4 Stars
|Mercure Hadleys Hobart Hotel - Previously Grand Mercure
|4 Stars
|Quality Hotel Hobart Midcity
|3.5 Stars
|Apartments at Woolmers
|3.5 Stars
|Waterfront Lodge
|3.5 Stars
|Travelodge Hobart
|3.5 Stars
|Best Western Balmoral Motor Inn
|3.5 Stars


text: I use our helpdesk software by assigning assets to printers. Everytime we put out an order for printers we make a purchase order and when we recieve the items we simply just select the recieve all option and it makes all the ink to assets. Then we can do a report to check what we have it and if we want to check a department of there printing we just view the printer.


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We offer a variety of services everything from daily dog walking to In Home Pet Care for all types of ani... more
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Pet owners from Portsmouth, Newington, Kittery and all of the NH seacoast are welcome at Lafayette Anima... more
Kittery Animal Hospital in Kittery, ME is a full service companion animal hospital. It is our commitment ... more
At Village Veterinary we provide care in a unique two-tiered approach, beginning with prevention and culm... more
Wendy's Lucky Paws brings over 30 years of experience in grooming and caring for dogs all over the Seacoa... more
Professional In-Home Pet Care for dogs, cats and other small animals. Overnight, day visits, dog walks, ... more
Personalized and Reliable in-home care for your pet in Portsmouth, NH. In business since 1997. more
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Training Your Dog can be frustrating. It's best to star... more
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Training Your Dog To Shake is a simple yet impressive t... more
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Teaching your dog to dance is an exciting trick.Read on... more
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submit news
Category: Investment, Biotech, Pharma
Biotech Stock Alert: SUNSHINE BIOPHARMA (OTCBB:SBFM) TAKES DELIVERY OF NEWLY MANUFACTURED BATCH OF Adva-27a AND PROCEEDS TO CONDUCTING BI0LOGICAL VERIFICATION OF THE COMPOUND'S ANTI-CANCER ACTIVITY
Ideas get bigger when you share them...
Visit this company: Sunshine Biopharma Inc.
Montreal, Quebec, Canada - September 4, 2012 (Investorideas.com Newswire) Sunshine Biopharma Inc. (OTCBB:SBFM), a pharmaceutical company focused on the research, development and commercialization of drugs for the treatment of various forms of cancer, today announced that it has taken delivery of an initial batch of Adva-27a from the Contract Manufacturing Organization (CMO) and has proceeded to conducting a series of requisite biological tests to ensure that the newly manufactured batch is identical to the original, not only in terms of chemical structure but also in terms of biological activity. Among the tests we will be performing are Topoisomerase II inhibition assays and cytotoxicity evaluations. The Topoisomerase II inhibition tests are already underway and the cytotoxicity evaluations will be initiated later this week. Adva-27a is Sunshine Biopharma's lead anti-cancer compound which has proven effective at killing Multidrug Resistant Breast Cancer cells (MCF-7/MDR) and Small-Cell Lung Cancer cells (H69AR).
"We are delighted to have advanced to this stage in our Adva-27a development program", said Dr. Steve N. Slilaty, Sunshine's President and CEO. "Biological testing is an important quality control procedure to ensure batch to batch identity in the manufacturing process", he added.
About Sunshine Biopharma Inc. (OTCBB: SBFM):
Sunshine Biopharma is a pharmaceutical company focused on the research, development and commercialization of drugs for the treatment of various forms of cancer. The Company's lead compound, Adva-27a targets aggressive forms of cancer.
Safe Harbor Forward-Looking Statements
To the extent that statements in this press release are not strictly historical, including statements as to revenue projections, business strategy, outlook, objectives, future milestones, plans, intentions, goals, future financial conditions, future collaboration agreements, the success of the Company's development, events conditioned on stockholder or other approval, or otherwise as to future events, such statements are forward-looking, and are made pursuant to the safe harbor provisions of the Private Securities Litigation Reform Act of 1995. The forward-looking statements contained in this release are subject to certain risks and uncertainties that could cause actual results to differ materially from the statements made.
For Additional Information Contact:
Camille Sebaaly, CFO
Sunshine Biopharma Inc.
Direct Line: 514-814-0464
Published at the Investorideas.com newswire
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At least 50 people have been injured in Bangladesh in clashes between protesters and police on the first day of a two-day strike called in protest at proposed changes to the country's election system.
Police detained nearly a dozen activists belonging to the Bangladesh Nationalist Party (BNP) of Begum Khaleda Zia, the former prime minister, and the Jamaat-e-Islami party during the strike which partially disrupted transport and business across the country on Wednesday.
The opposition parties called the strike to protest against the abolition of a system of holding national elections under a non-partisan caretaker administration.
The injured included Jainal Abedin Faruk, a senior BNP leader, who Kazi Wazed Ali, a police officer, said attacked security forces trying to prevent a street march in the capital, Dhaka.
Mirza Fakhrul Islam Alamgir, the BNP's acting secretary-general, told reporters "the attack on Faruk was intended to kill him and this showed how vindictive a government can be towards its rivals".
Sahara Khatun, the home minister, expressed her sorrow over the injuries to Faruk, a member of parliament and the BNP's chief whip, but she said "political leaders should also behave properly with law enforcers".
Clashes occurred in Dhaka, the southern port city of Chittagong, and a few other cities in the north and the east. At least 10 vehicles were torched in the capital overnight.
Bangladesh's parliament on June 30 amended the constitution to abolish the caretaker system introduced in 1996 to try to end the violence and fraud that have often marred voting in the South Asian country.
The Bangladesh Supreme Court in May also ruled the caretaker system unconstitutional.
The BNP, which is the country's main opposition party, is also incensed by the framing of charges against Khaleda's elder son, Tareque Zia, this week for involvement in a 2004 grenade attack on a political rally addressed by Sheikh Hasina, then opposition leader and current prime minister.
Hasina and Khaleda have dominated the nation's politics for two decades, and analysts fear that their renewed confrontation could plunge the country back into turmoil.
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Dash cam video shows a deputy sobbed with regret after he fired shots at a 70-year-old Vietnam veteran after he thought the man's cane was a shotgun. The incident happened during a traffic stop in South Carolina.
In the dash cam video, the deputy, identified as Terrence Knox, calls out a warning before firing several shots at Bobby Canipe after Canipe got out of his truck during the traffic stop to retrieve what the deputy thought was a shotgun from the bed of his pickup.
Watch the latest video at video.insider.foxnews.com
"Drop the gun!" yelled Knox. Canipe responds. "It's a walking stick."
One bullet struck the veteran in the stomach, but according to officials he will survive.
The deputy, realizing his mistake, hurries to help Canipe while apologizing, ""I am so sorry."


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|The Department of Homeland Security, state police and Watertown detectives worked on an investigation that led to child pornography charges against an Oakville man. Republican-American
WATERTOWN — An Oakville man was charged with voyeurism and possessing child pornography this week as part of a broad federal investigation into a suspected pedophilia ring that is alleged to have swapped tips about how to drug children in order to take their pictures.
Jeffrey Cathcart, 37, of 49 North St., is being held on a $200,000 bond after an extensive investigation that involved state police, Watertown detectives and the U.S. Department of Homeland Security.
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riderdie wrote on Jun 13, 2014 6:47 AM:
" What a scumbag. Lock him up. "
kipper wrote on Jun 13, 2014 7:53 AM:
" Nothing a good castration can't fix. That should be the punishment for all of these freaks, unfortunately there may be a law or two against such things. Thanks liberals "
michaelk wrote on Jun 13, 2014 8:50 AM:
" if you mess with children you should be sentenced to death row .. just my opinion .. "
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