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- *A MUST SEE* Beautiful 3Bd 3bth Home, 15 acres with Pond
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subset: commoncrawl


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Hi Folks,
My name is Derek. I live in Canada right now (don't get me started), but there is a good chance I am moving to Israel soon. I love shooting, am a good shot and am excited at the prospect of living in a country that will allow me to own a handgun with minimum hassle. As well, I am considering CCW for certain times and locations (while crime isn't an issue over there, we all know how things are...).
I hope to learn a lot as well as contribute.


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Recent comments
- Let them all go - back to god
- February 25, 2014 - 02:17
If you can let old people, for lack of options, care and respect, via legislation, the right to die, and now young people too, in Belgium, well by, I think these incarcerated Children of God have the same rights, and should have access to all the same opportunity to die, and more. They should not be forced to live in misery as a makework project for hairy-arsed babysitters 'nepotized' by the judiciary. They should be reminded every morning, before they get their bowl of mush, that God, or Jesus, or Rocky and Bullwinkle, or blackness, if they prefer (or Dante motif is an option as well, for the more intellectual felon) are waiting for them on the other side, and it is painless, easy, no trouble, easy on the family, the state – a good choice. Before bed, like the snacks in the Health Science, someone goes the rounds, just checking to see who needs to be euthanized before bedtime. They deserve the right to choose death.
- A Citazin
- February 22, 2014 - 21:38
@Carl by the sound of it you know some of the scum at the HMC but when you home is violated now all of a sudden you have to put a security system in your home to protect your family now all of a sudden you have a extra bill a month,now keep in mind a violation that takes place while your family are sleeping.Why do you think you pay more every year for car insurance when in the thirty years of driving not once having a claim,but every year we pay more.So before you tell me to stop whining and get a better job maybe you should goto the pen and coach these people on how to get employment.
- A citazin
- February 22, 2014 - 09:10
Yes the guards do deserve a safe place to do their job,how many inmates are there over and over again,and most would say some are there because of petty crimes,but let me tell you drunk driving,arm robbery,selling drugs are not small crimes this is serious I don't want my children sold drugs,I don't want my kid hit by a drunk driver,I don't want my tax dollars to pay for these animals,they should be hand cuffed to their cell and only given two feet to walk around.As you know sir most of these inmates are the same repeated offenders they know the system.Go to Mass service what a joke most never seen insid a church before.Sorry I sound so cold but I get tired of paying for these people when most paydays I stuggle to pay my bills.The guards,staff,volunteers deserve better working conditions,but most what's serving time there don't deserve a meal let alone a shower..
- Jeff
- February 22, 2014 - 12:22
Kind of like Wild Rose, but not quite a bright.
- Carl
- February 22, 2014 - 12:36
If you're struggling to pay your bills, stop whining and get a better job.


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Sounds Like: Radiohead, Coldplay, Beck, Talking Heads, Nirvana
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Definitions provided by:
Mouth, Teeth and Oral Health Glossary
Terms used to explain Mouth, Teeth and Oral Health can sometimes be confusing. To help you fully understand the articles and features related to this very important health topic, we have compiled a glossary of terms that can help.
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It's very small, so you pack it anywhere. Comes with a handful of adapters but I only carry around the standard MicroUSB-USB cable that can not only charge the battery but also use it afterwards to charge my phone! And it's retractable, so that's also good. It can charge my phone (Galaxy S) almost back ... Read more
Small, light-weight device. Can be charged with same cables and adapters that it is delivered with. Plenty of adapters included, but only for different smart phones. Good enough to re-charge my small GPS tracker within a few hours, not tried on my mobile phone yet. Not sure about the quality of the battery ... Read more
- Charge level test. Very useful. - Lot of adapters. - USB output. Even if there isn't needed adapter You can use Your own. - Micro USB input. Mini USB suits more. - Even if there is declared PSP-compatibility in manual there is still no special adapter though, so i've build my own. - Too thick wires.... Read more
-It's a very versatile battery pack thanks to USB port. -Useful to charge my iPhone 4 (capacity equivalent to 1,5 full charged battery) and to give a push on iPad 2 (1/3 of full charge) -It came with a lot of adapters, allowing the use with an infinity of devices. -Past one year of intensive use, the ... Read more
*The test button actually works. *Can charge any product that connects to a USB (USB Output directly from the battery) *Great capacity (can charge both my ipod and my iphone 2 with a single charge) *Great accesories for cell phone charging * Comes with retractive cables (both mini usb and the one for ... Read more
. Charges my Nokia N82 from flat to full 3 times . Really light and easy to carry on your pocket . LEDs indicate the power level . Charges off USB . The connectors are bad quality. I recommend buy a more sturdy brand, for the Nokia you can find better adapters (USB -> power) Perfect backup battery ... Read more


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ASUS VS197DE LED monitor - 18.5" Resolution 1366 x 768 pixels, Inputs VGA, Response time 5 ms
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As you may have already heard, the Banksy piece on LaBrea was removed yesterday morning. Jet Set Graffiti provides the complete story, as well as the above video exclusive. "On the morning of April 13th, 2010, Ace Gallery owner Doug Christmas organized the removal of a Banksy painting from it's installation location at 410 S. LaBrea Ave. in Los Angeles. This "Banksy" has not been authenticated by the artist and is being sold as an unauthorized gallery artwork. Do NOT support this sale; the work is now considered counterfeit." More information at www.jetsetgraffiti.com
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- Running
- Cycling
- Triathlon
- Fitness
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About This Activity
Red Bull Trail Daze - Three Courses, One Objective. Choose Your Path. Registration ends December 9th at 12PM. Red Bull Trail Daze is a unique trail running competition that challenges runners to race from point A to point B with different paths to choose from. Courses will be designed in the Palos Forest Preserve in Willow Springs, each that will progressively become more challenging along the way.
Organizer Info
Map & Direction
Saturday, December 10, 2011 @ 10:00 AM (CST)
Pulaski Woods, Palos Forest Preserve Willow Springs, IL 60480Get Directions


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The apartment is very close to the train station which made getting in and around the city very easy. The room wasn't the biggest but for the price we paid we weren't expecting anything huge, the kitchen was very well equipped for a small studio so makes it easy if you want to cook food. It's not within waking distance to too many sights but the train is so close so it's easy to just jump on that. There are alot of cafes/restaurants near and also a grocery store. Staff were very friendly and he - Anonymous
nice room with a little fridge, stove, microwave etc. They gave two bottle of water and two bottles of beer a day for free which is very rare in Europe. This was the best placed we stayed during our entire trip accross europe. - Andrea
The wiev from room, room in color, the friendly receptor. - Irena
The stuff was very friendly, the breakfast very good, the room clean and comfortable and the hotel is very close to the underground. - Vasileios
容易找到,离火车站近 - Ying
See above - the hotel is perfect. Moreover, I appreciate especially the friendly atmosphere. - Svetlana
It is a quiet hotel at the heart of Munich (5 metro stations from thecenter). The rooms are big and comfortable and the stuff is veryhospitable. Breakfast is very good served in a cozy hall. - David
Cleanliness, Good room design and the relaxing area. - Saad
价格适宜 - Changqing
The room was very large, there was even a small kitchen area with some free beverages in the refrigerator. The room was very clean, and modern-looking. The hotel is close to the U-Bahn and is extremely easy to reach from the main station. The staff was very professional and friendly and spoke great english and also russian. There was no problem with checking in late at night. - Anonymous
Had a great stay here with one of my friends. The staff was extremely helpful, the room exceeded my expectations of cleanliness and space, and the area was key. With bus 55 right outside easily taking you to the trains. Best hotel I've stayed at so far with my four months of traveling abroad. - Gina
Staffs were prompt in their responses back to emails. We had no problems finding the hotel. It was just 5 mins walk from the U bahn station Thalkirchen. Breakfast was good but with limited choices. If u got no universal adapter, u can borrow it with no cost. - Anonymous
Price, location in Munich, Friendly staff and very nice room - Anonymous
离慕尼黑中央火车站比较近,步行就可以到达市中心的主要景点 - Yifei


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Admin website ftp
I have at last installed ispconfig but what are the ftp settings and email settings for my own site, ie the host domain.
I know that the document root is /var/www/html/
Sorry if this question is very basic but I am lost.
You have to create that web site in ISPConfig, and on the tab "User & Email" you can create users and email addresses. The usernames and passwords are valid for ftp also.
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The Defense Security Cooperation Agency notified Congress July 9 of a possible Foreign Military Sale to Saudi Arabia of 30 Mark V patrol boats and associated equipment, parts, training and logistical support for an estimated cost of $1.2 billion.
The Kingdom of Saudi Arabia has requested a possible sale of 30 Mark V patrol boats, 32 27mm guns, spare and repair parts, support equipment, personnel training and training equipment, publications and technical documentation, U.S. Government and contractor engineering, technical, and logistics support services, and other related elements of logistics support. The estimated cost is $1.2 billion.
This proposed sale will contribute to the foreign policy and national security of the United States by helping to improve the security of Saudi Arabia which has been, and continues to be, an important force for stability in the Middle East. This sale of Mark V patrol boats will give the Royal Saudi Naval Forces (RSNF) an effective combat and threat deterrent capability to protect maritime infrastructure in the Saudi littorals. This acquisition will enhance the stability and security operations for boundaries and territorial areas encompassing the Saudi Arabian coastline.
The purchase of Mark V patrol boats represents an upgrade and modernization of the RSNF's existing patrol boat capability. The proposed sale will enhance interoperability between the U.S. and the Kingdom of Saudi Arabia and will contribute to the stability in the Kingdom of Saudi Arabia and the region. The Mark V patrol boats will provide additional capability to rapidly identify, engage, and defeat maritime security threats in the near-offshore region of the Saudi littorals. The boats will be used primarily to patrol and interdict intruders in Saudi territorial seas, and recognized economic exclusion zones. Saudi Arabia will have no difficulty absorbing these additional boats.
The proposed sale of this equipment and support will not alter the basic military balance in the region.
The principal contractor for this effort has not yet been determined. There are no known offset agreements proposed in connection with this potential sale.
Implementation of this proposed sale will require an additional three to four U.S. Government and contractor representatives to Saudi Arabia for a period of seven years to provide logistics and technical support and warranty work during delivery of the boats.
There will be no adverse impact on U.S. defense readiness as a result of this proposed sale.
This notice of a potential sale is required by law and does not mean the sale has been concluded.
Related Articles
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Keywords:raytheon saudi arabia patrol vessel, Navy MK V patrol boats, private Mark 5 boats, saudi patrol boat
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Search results for rihanna
Color TV: A List Of The Most Popular Black Shows And How They Rank With White Folks
Ever wonder what the "other" folks are watching?
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This is 18 year-old Matthew Herrmann, and he is a racist douchebag who's going to jail for a LONG time!
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Most Popular
.- Archbishop Odilo Scherer of Sao Paulo said last week, "The presence of the new communities and movements is a great treasure today for Latin America and the Church."
In statements to CNA, the Brazilian archbishop, who is participating in the 5th General Conference of the Latin American Bishops' Council (CELAM), said the phenomenon of the movements and new communities "is ever more significant in the life of the Church because it is animated by a mystical strength according to each one's charism."
He said the CELAM conference in Brazil is giving particular attention to the new movements and communities, encouraging them to continue to form disciples and missionaries that have an active presence in society and work for the transformation of the world "so that our peoples may have life in Him."
Asked if the Church would accept the legalization of abortion in some cases, as has been suggested by liberation theologian Fr. Betto, Archbishop Scherer was clear in his response: "No. The position of the Magisterium of the Church regarding abortion is clear. Legalizing abortion in some cases means going against a basic principle: the human person, human life, must always be respected."
Human life "must never be sacrificed for some other interest, whatever that interest may be. Human life has a primary value and therefore it must be respected always, and therefore I don't think the Magisterium of the Church can be in agreement with the legalization of abortion in some cases," Archbishop Scherer stated.
* The number of messages that can be online is limited. CNA reserves the right to edit messages for content and tone. Comments and opinions expressed by users do not necessarily reflect the opinions or beliefs of CNA. CNA will not publish comments with abusive language, insults or links to other pages


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San Antonio Spurs (13-3)@
Orlando Magic (5-9)
Wednesday, November 28
Amway Center
Attendance: 17,271
Game Leaders
20 pts, 67 fg%, 100 ft%
16 pts, 50 fg%
7 reb
6 reb
8 ast
4 ast
Orlando, FL (Sports Network) - Manu Ginobili drained six 3-pointers en route to a game-high 20 points as the San Antonio Spurs thumped the Orlando Magic, 110-89, on Wednesday at Amway Center.
Ginobili added five assists, Gary Neal finished with 19 points and Tim Duncan contributed 15 points and five blocks for the Spurs, who have won five straight and improved to 9-1 on the road this season. Tony Parker donated 14 points and eight assists, while DeJuan Blair gave 11 points and six boards in the victory.
"Our offense has been clicking," Parker said. "We've been sharing the ball well."
Arron Afflalo netted a team-high 16 points and grabbed five boards, while Jameer Nelson had 14 points for the Magic, who have lost four of their last six. Glen Davis donated 12 points in the setback.
Orlando had the hot hand early on, hitting seven of its first 11 shots en route to a 14-10 lead with 5:33 on the first-quarter clock.
But the Spurs responded with 13 straight points to take a 23-14 lead with 2:37 left. Ginobili drained a pair of treys during the run, which finally came to an end when Gustavo Ayon put home a layup.
Ginobili buried two more 3-balls over the final minute-plus of the quarter and finished the session a perfect 4-for-4 from beyond the arc for 12 points as the Spurs held a 29-18 advantage after 12 minutes.
San Antonio maintained its lead for the duration of the second quarter. A running bank shot by DeQuan Jones with 6:48 to play before the half cut the Spurs' lead to five, but the visitors scored the next five points to build the advantage back to double digits.
A deep deuce by E'Twuan Moore late in the frame made it a nine-point game, but Parker put in a reverse layup and Duncan buried a trey at the horn to put the Spurs up 55-41 at halftime.
A jumper by Davis early in the third quarter made it an eight-point game, but San Antonio was in complete control over the remainder of the game, leading by as many as 25 en route to the easy victory.
"They're a great team. They've been great for years," Nelson said of the Spurs.
Ken Mauer Courtney Kirkland Olandis Poole


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John Barrymore is in with Clark Gable it's amazing movie.
Thank you Ruby Miller
The movie has a semi-documentary style that is somewhat interesting, breathtaking scenery at one point, and lots of good actors, but Clark Gable spends all his time sitting in a... Read morePublished 4 months ago by Phyllis Hirshleifer
Being a great Barrymore fan I enjoyed the film very much. A great period piece. I wish they would release all the lionel barrymore films from 1930 onward to DVD. Read morePublished 8 months ago by steve Knight
WOW, great cast and story based on history - no idea how the "over-night" delivery service began - but I do now. The good ole days were hard ... Read morePublished 8 months ago by Micky
While some of the special effects shots are poor and the script is not the best, the aircraft used in the film are spectacular. Read morePublished 8 months ago by Alan K. Sumrall
For a 1933 production, this was well worth viewing. Strong acting, wonderful flying sequences, rare story. Read morePublished 9 months ago by M&M
Although not on the story quality of MGM's Grand Hotel or other similar All Star Casts, the many major stars, sets, story line, airplane make up for any loss of script or story... Read morePublished 21 months ago by Richard Davis McLeod
After the successful production of MGM's lavish 1932 star-studded classic "Grand Hotel," MGM's "Night Fight" tries to recapitulate on this success but falls short in this... Read morePublished 22 months ago by Rama Rao


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The committee heard again and again that elderly individuals place key importance on their ability to have access to "their" traditional providers with whom they have developed a personal relationship.
The importance of considering the effect of personal and cultural factors on access is heightened by the changing demographics of the U.S. population. The committee heard that certain Medicare beneficiaries (particularly low-income and minority groups) may be at significantly higher risk of not being able to continue to be seen by their traditional network of providers in an environment of managed care. Because of the lower socioeconomic status of many individuals who are members of minority groups, a managed care plan may be the only delivery option that is affordable.
As managed care plans continue to develop they will have an increased responsibility to improve access for underserved populations. The committee believes that health plans should be held responsible for serving their entire service area without compromising access or quality of care. The committee found that some providers who have served their communities for many years or who are part of essential community provider networks, have not obtained the credentials required by some managed care organizations either because of institutional racism or common practice within their specialty to forego board certification. It is important that health plans develop several measures of clinical competence that are sensitive, valid, and reliable in their ability to assess clinical competence through both outcome and process indicators. The committee heard testimony that managed care plans often do not disclose their credentialing standards and policies. At the very least, such disclosure should be required. The committee lauds the efforts under way in HCFA, PPRC, a number of health foundations and other groups to track and address key issues that could arise in monitoring access to care under a restructured Medicare program.
Broad access for Medicare beneficiaries is key. The committee recommends that the federal government ensure that there is adequate access and choice of plans for individuals in all


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The Bachelor Canada is holding a casting call in my hometown this week, inviting singles to take one "Last chance for romance!" Men who are not tapped to be the next bachelor, or women who are not one of the 25 lucky ladies, need not despair; the format of the show creates an environment in which finding lasting love is extremely unlikely.
Of the 17 completed seasons of the Bachelor USA, 11 couples did not make it to the three-month mark in their relationship, only three made it beyond a year, and of those only one couple eventually married (just last week!).
There is a reasonable market explanation for the inability of the show to create lasting relationships; one the producers of the Canadian show might want to take into consideration when choosing the format for the upcoming season.
It comes down to the mating strategy a man will choose when he is trying to maximize his biological fitness – to have as many children as possible – and that choice of strategy depends on the level of competition he faces from other men.
When single women are abundant, and there is relatively little competition from other men, the best strategy is for a man to seek out a series of short-term sexual relationships in the hope that each one might produce a child.


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Action-571 Propose new SC 1.8.y Open in a new window/tab:: User can specify that links (frames?) are opened in a new user agent tab or window instead of the current tab/window. -- Jim Allan, Accessibility Coordinator & Webmaster Texas School for the Blind and Visually Impaired 1100 W. 45th St., Austin, Texas 78756 voice 512.206.9315 fax: 512.206.9264 http://www.tsbvi.edu/ "We shape our tools and thereafter our tools shape us." McLuhan, 1964Received on Thursday, 10 November 2011 17:38:54 GMT
This archive was generated by hypermail 2.2.0+W3C-0.50 : Thursday, 10 November 2011 17:38:54 GMT


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First day? Welcome!
True or False: You're ready to achieve your higher education and career goals? That's
what we thought. And that's what we're here for. From college to career-we've got
you covered. So get going! Your future's waiting.
901 South National Avenue
Springfield, MO 65897
Phone: 417 836-5331
Fax: 417 836-6888
Audiology (AuD)
Communication Sciences and Disorders (MS)
Cell/Molecular Biology (MS)


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Connect and grow with us!
Still struggling to hang on? Get to the Truth!
Frustrated with a lack of spiritual growth in your life? Still dealing with the same old problems, habits, and hurts?
Whether your problems are life-controlling, historic abuse affecting today or some secret sin, God cares and He can help. He wants to help, but He will not violate your freewill.
If you want God to do His part, then do your part. Come let us show you how to position yourself before God in your daily walk for the deliverance, change, and victory you've been longing for. Get to the Truth!
Look no further!
The Truth Church is a place for your family to…
Connect with Christ
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Go for Christ
The Goal of Christianity
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Implementing video acceleration on intel chips with the video Acceleration API
Fast Frame
Improve video performance with hardware video-accelerated decoding using the video Acceleration APi.
For many years now, Intel has developed processors with built-in video acceleration. This hardware video acceleration has the effect of reducing latencies between the Graphical Processing Unit (GPU) and the processor cores. The interface also provides access to features built into the GPU, such as H.264 encoding, scaling, MPEG decoding, and so on.
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TGXf Project Warns of File Transfer through Screen Pixels
Weird data transfer technique avoids all standard security measures.
Industry Giants Announce a Fix for the Password Mess
FIDO alliance declares the beginning of the end for old-style login authentication.
Poll: Vote for the Best Open Source Software for Photographers
The Linux New Media Awards have honored the most significant products, projects, people, and organizations for open source/Linux every year since 2000.
Debian Gets Forked
Legendary Uber-distro splits over the systemd controversy.
Linux Mint Project Releases Mint 17.1
New LTS version offers many refinements for the Cinnamon and Mate desktops and significant improvement under the hood.
CeBIT Open Source Forum Call for Papers
One of CeBIT's most successful forums returns in 2015.
TOR Network Isn't So Anonymous
A new study says it is possible to unmask 81% of TOR users.
.NET Goes Open Source
Redmond joins the revolution by turning the .NET Core Runtime into a GitHub project.
Drupal Advisory Unleashes a Torrent of Attacks
Users only had 7 hours to update before the intrusions started.
HTML5 Gets Promoted to a Standard
It's official: The new web arrives


text:
12 Years A Slave at Curzon Soho
From to
Solomon Northup is a free black man, who lives in 1841 Washington City with his wife Anne, daughter Margaret and son Alonzo. An encounter with two seemingly respectable gentlemen changes Solomon's life forever. He wakes up in chains and learns he has been sold into slavery. For 12 hellish years, he endures physical and mental abuse, clinging to the hope that he might be reunited one day with his loved ones.
- Director:
- Steve McQueen
- Duration:
- 134
- Country:
- Year:
- 2013
Venue Details & Map
Curzon Soho
- Address
93-107 Shaftesbury AvenueLondon ChinatownLondonW1D 5DY
Book a taxi on +44 (0)844 556 0706 or with London's biggest car service app


text:
Click on a thumbnail to go to Google Books.
Evolution: The Story of Life on Earth (original 2011; edition 2011)
by Jay Hosler, Kevin Cannon (Illustrator), Zander Cannon (Illustrator)
Work details
Evolution: The Story of Life on Earth by Jay Hosler (2011)
Compact | Rate recommendations
No current Talk conversations about this book.
Underhållande och tydlig. Lyckas förmedla mycket av det vetenskapen vet om hur liv har uppkommit och jorden och utvecklats till oss på bara 150 sidor. Det känns som att det borde funka helt utan förkunskaper, men serien innehöll ändå en hel del som var nytt för mig trots naturvetenskaplig / teknisk bakgrund.
Graphic novel. So-so
A quick and easy, graphic novel treatment of the theory of evolution. It is relatively thorough, but the cartoon treatment limits it somewhat. The book presents the basics of the theory, but is a bit light on the evidence presented. Overall, it is a great starting point for people who don't want to slog through a dense, scholarly book on the topic, and might be useful to generate further interest in the subject.
Z really liked this . . . it took several weeks of lunch-time reading to finish it. It's a hard-core look at genetics and evolution, and VERY in-depth/science-y. But funny at the same time.
On the planet Gargal, Bloort, a scientist and servant of the king, invites King Floorsh and Prince Floorsh to see a hologram presentation he created about his research on the history of life on Earth. Bloort then begins to weave a tale that teaches the fascinated young prince about the evolution of earthly life beginning over four billion years ago. This remarkably detailed story reads like a textbook written in the form of a graphic novel. The author and illustrators do not miss a fact and provide a complete account of what middle and high school students need to know about this topic. They discuss the evolution of humans, the various forms of selection, many extinction events throughout history, etc. There is a great deal of information packed into 142 pages and the story moves along quickly, so those unfamiliar with the terms and topics may have difficulty following along. This graphic novel, however, is a wonderful tool for science teachers as it presents the material in a way that may appeal to their students more than a standard textbook. This graphic novel is recommended for middle and high school science collections. The students will hopefully be just as enthusiastic to learn about evolution as Prince Floorsh.
References to this work on external resources.
Wikipedia in English
Amazon.com Product Description (ISBN 0809094762, Hardcover)
An accessible graphic introduction to evolution for the most science-phobic reader
Illustrated by the brilliant duo Kevin Cannon and Zander Cannon, this volume is written by the noted comic author and professor of biology Jay Hosler. Evolution features the same characters introduced in the highly regarded The Stuff of Life: A Graphic Guide to Genetics and DNA, now here to explain the fundamentals of the evolution of life on earth. On the heels of explaining to his planetary leader the intricacies of human genetics in The Stuff of Life, the intrepid alien scientist Bloort-183 is charged in this sequel with covering the wider story of evolution. Using the same storytelling conceit that Plenty magazine declared "so charming that you won't even notice you've absorbed an entire scientific field" and that caused Seed to pick The Stuff of Life as a best book of 2008, Evolution brilliantly answers Wired's demand, "What's the solution to America's crisis in science education? More comic books!"
Evolution, the most accessible graphic work on this universally studied subject, takes the reader from earth's primordial soup to the vestigial structures, like the coccyx and the male nipple, of modern humans. Once again, the award-winning illustrations of the Cannons render the complex clear and everything cleverly comedic. And in Hosler, Evolution has an award-winning biology teacher whose science comics have earned him a National Science Foundation grant and an interview on NPR's Morning Edition.
(retrieved from Amazon Mon, 30 Sep 2013 13:28:07 -0400)
A graphic-style introduction to evolution, covering everything from Earth's primordial soup to the human body's vestigial structures.
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Shrimp, Artichoke, & Spinach Dip
Recipe Category: Appetizers, 15-minute Meals
PREP TIME: 3 minutes
COOK TIME: 10 minutes
MAKES: 4 servings
DEFROST spinach in microwave according to package directions and drain well.
COOK TIME: 10 minutes
MAKES: 4 servings
1 pk(12 oz) SeaPak® Shrimp Scampi, frozen
18.5 oz can artichokes, quartered and well drained
19 oz carton spinach, frozen
1⁄4 cItalian bread crumbs
3 Tsour cream
3 Tpre-cooked bacon crumbles (3 slices of bacon cooked and crumbled)
1 cParmesan cheese
DEFROST spinach in microwave according to package directions and drain well.
HEAT large skillet for 1 minute on medium high. Add shrimp and sauté for 7 minutes.
ADD artichokes, spinach, bread crumbs, sour cream, bacon crumbles and cheese to the skillet with shrimp. Stir well to incorporate all ingredients. Cook an additional 3 minutes or until cheese is melted and shrimp is fully cooked.
POUR mixture into serving dish and serve with crackers.
Recipe courtesy of Robin Miller.
This recipe features
Shrimp Scampi, 12 OZ


text:
this wiki
Redirected from Villagers
The Villager (known as the Settler in Age of Empires III) is a common civilian unit for every game of the Age of Empires series. They are the backbone of all civilizations, and arguably the most important units in the game as they are able to collect natural resources such as Food, Wood, Stone, Gold, and Coin. Villagers are also able to construct buildings and repair them, along with boats and siege weapons in some AoE games. If the civilization is attacked, they can defend their towns, but only in dire need.
Villagers are so important because as long as they live they produce resources, which are constantly required. One of the best ways to disrupt an enemy's strategy is by killing their villagers at the beginning of the game, as they will have to make new ones, costing them time and resources. Cavalry are a fast and effective way to deal with Settlers or Villagers, as they are good for hit and run tactics and can mow down Villagers quickly.
Age of Empires Edit
- Main article: Villager (Age of Empires)
Age of Empires II Edit
- Main article: Villager (Age of Empires II)
Age of Mythology Edit
In Age of Mythology each of the four civilizations has its own type or types of villager.
Greek and EgyptianEdit
Greek Villagers and Egyptian Laborers are similar to their counterparts in thier AoE series. However, Greek Villagers can be assigned an additional task, praying at a Temple to generate favor, and Egyptian Laborers gather and build more slowly.
- Main article: Villager (Age of Mythology)
- Main article: Laborer
The Norse have two types of villager: Gatherers and Dwarfs. Neither of these units can build or repair buildings (this is left to Norse Infantry), and both can be converted into Ulfsarks. Dwarves cost gold instead of food and are better at gathering gold, but not as good with other resources.
Atlantean Citizens have donkeys with them which enable them to deposit resources without visiting a drop point; they are expensive but effective workers. Citizens can be promoted to Citizen Heroes, after which they have better building and gathering rates. Like other heroes, Citizen Heroes can collect relics.
- Main article: Citizen
Age of Empires III Edit
In Age of Empires III most European villagers are known as Settlers, but the Native American and Asian civilization equivalents are still called Villagers. They function the same as in previous games, but no longer require dropsites to deposit resources.
Even with all upgrades, settlers are too weak to fight very many military units or treasure guardians. The French civilization have stronger settlers called Coureur des Bois which can collect resources faster and can also double as treasure gatherer if the Home City card Northwest Passage is sent. The German civilization also has a stronger settler unit called the Settler Wagon.
Most settlers and villagers deal much more damage in melee than at range, multipliers may allow them to do more at range only after the Market upgrade has been purchased and only against Artillery, Pets and Treasure Guardians. The Coureur is an exception to this, having 8 ranged attack instead of 3 but a x0.25 multiplier versus settlers and villagers. All villagers only inflict 2 damage against each other at range, 3 following the market upgrade and around 5 for Unction boosted Spanish settlers. With melee attacks (lacking any negative/positive multipliers) they are easily capable of killing one another, Explorers and non-upgraded infantry units granted they gang up on it and can catch it. Sometimes this may be a better option than fleeing with them, depending on the size and type of the enemy force harassing them and the number of settlers or villagers.
- Note: Settlers of any type cannot use ranged attacks if the target is too close to them, they will automatically use melee attacks. This is mostly a problem only for the French and Cree Coureur des Bois.
They are especially useful against enemy pets (the Coureur against guardians with a card shipment), as all pets have a negative x0.1 multiplier versus villagers with exception to Fluffy, the Explorer Dog (no negative/positive multipliers), War Dogs (negative multiplier of x0.3) and Tigers (negative multiplier of x0.2). Only the cheat unit Fluffy, or Unction enhanced pets pose any type of real threat to Villagers.
- Main article: Settler
- Main article: Coureur des Bois
- Main article: Settler Wagon
- Main article: Villager (Age of Empires III)


text:
I just checked several of my webpages that were validated within the last week only to see they had failed! Reason given was --I think-- a misread of my charset=ISO-8859-1. The error message reads something like, "can't validate ISO-88059-1" which is NOT what I have. Is this a temporary glitch in the system or ??? JoanneReceived on Sunday, 22 July 2007 18:11:12 GMT
This archive was generated by hypermail 2.2.0+W3C-0.50 : Wednesday, 25 April 2012 12:14:25 GMT


text:
Nutshell - Alice in Chains
Nutshell - Alice in Chains
|Sponsored Links
Smells Like Teen Spirit-Nirvana
Utah Jazz Sim GM2003: 28-54, 11th in the West
2004: 33-49, 10th in the West
2005: 35-47, 12th in the West
2006: 45-37, 8th in the West, 1st Round loss
2007: 57-25, 3rd in the West, 1st Round loss
2008: 30-52, 12th in the West
2009: 47-35, 6th in the West, WCF loss
2010: 47-35, 7th in the West, WCF loss
2011: 5-77, 14th in the West
2012: 38-44, 10th in the West
2013: 41-41, 8th in the West, 1st Round loss
2014: 50-32, 2nd in the West, 2nd Round loss
Favorite song? That's hard
Too many
I guess I would choose Hurt Me Soul - Lupe Fiasco, or I Can by Nas
Stickwitu- PCD
Baby Love- Nicole Scherzinger
What I Got- Sublime
"[He's] a really complete player... There's not really any weaknesses to his game." - Bill Belichick on Andrew Luck
"Vontae Davis looks like Bumgarner of the San Francisco Giants. He's throwing a no hitter out there tonight" - Jon Gruden
Time is Running Out: Muse
Slap Hands!
is here http://www.prosportsdaily.com/forums...d.php?t=265925
just messing with you
|Sponsored Links
Invincible - Muse
Or legitimately any song Muse hase ever written...
Hotel California - The Eagles
grindin- alishay
trying to find a balance- atmosphere (basically, holy ****), also sunshine by atmoshphere
Hurricane- Bob Dylan
Three Little Birds- Bob Marley
Stronger- Kanye West
When I'm Gone- Eminem
Interstate Love Song- Velvet Revolver
Rock Jesus of Suburbia - Green Day (Kicks *** live)
Alot of old Green Day
Rap The Game - Dreams
Jay-Z @ UGK - Big Pimpin
Aint No Love- Jay Z
New York City- Jay Z & Camron
I can't do it I like too many songs!
|Sponsored Links


text:
Peg's picks
(4 Recipes) Created by PAFORNEY
Recipes in this Collection
This is a recipe i put together last night with the veggies I had in the fridge and my husband and daughter LOVED it. Very yummy over ride pilaf or couscous. You can add chicken or fish to make this a non-veggie meal
Submitted by VEGGIEDOC4LIFE
Carbs: 15g | Fat: 7.1g | Fiber: 5.2g | Protein: 3.7g | Calories: 121.2
Incredible! 5.0/5
(4 ratings)
Based on recipe from Barefoot Contessa
Submitted by MANDY6488
Carbs: 7.5g | Fat: 3.3g | Fiber: 0.9g | Protein: 55.2g | Calories: 340.3
Incredible! 5.0/5
(4 ratings)
My mother makes these and are delicious. I had no idea how many calories I was taking in when I was eating them. Not sure if the calories on the wings is accurate.
Submitted by SASSDUKE
Carbs: 3.6g | Fat: 2.2g | Fiber: 0g | Protein: 6.6g | Calories: 61
Incredible! 5.0/5
(7 ratings)
Submitted by SXFREDER
Carbs: 9.9g | Fat: 14g | Fiber: 0.4g | Protein: 43.4g | Calories: 339.7
Send this recipe collection to a friend - http://recipes.sparkpeople.com/cookbooks.asp?cookbook=588931


text:
definition of Wikipedia
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|Casey Kotchman
|Cleveland Indians – No. 35
|First baseman
February 22, 1983
St. Petersburg, Florida
|Bats: Left
|Throws: Left
|MLB debut
|May 9, 2004 for the Anaheim Angels
|Career statistics
(through July 31, 2012)
|Batting average
|Home runs
|Runs batted in
|Career highlights and awards
Casey John Kotchman (born February 22, 1983, in St. Petersburg, Florida) is an American Major League Baseball first baseman for the Cleveland Indians. He is known for setting the record for having the most consecutive put-outs without committing an error.
He was on the 2001 national champion team of Seminole High School in Seminole, Florida.
The Anaheim Angels selected Kotchman with the thirteenth overall pick in the 2001 Major League Baseball Draft.
In 2006, the Angels moved 2005 starter Darin Erstad back to center field, announcing that Kotchman would likely open the season at first base. After struggling in his at-bats early in 2006 because of mononucleosis, Kotchman was placed on the disabled list in early May. In 2007, Kotchman proclaimed himself fully healthy and proved it by winning the Angels' opening day first baseman job for the second straight year.
Kotchman missed 128 games in 2006, as he was kept away from the field with complications stemming from mononucleosis. After a spring in which the Opening Day starting first baseman batted better than .400, he hit just .152 with one home run and six RBI in 29 games with the Angels. Kotchman tried to return to the field, but his rehab assignment was cut short when he experienced dizzy spells in July while playing for Triple-A Salt Lake. He spent much of the rest of the season at home in Florida, performing cardio exercises.
In 2007, he was one of seven Angels regulars to hit over .290 (most in majors) and established career highs in several offensive categories including batting average (.296), home runs (11) and RBI (68). He also hit .320 at Angel Stadium and .274 on the road. He had a 7-game hit streak (7-8/31-8/7), his 4th seven-game streak of season (each matching a career-high). He left the June 16 game against the Los Angeles Dodgers in the 7th after being hit on the helmet by a pickoff throw at second base by Dodgers catcher Russell Martin and was diagnosed with a mild concussion and received three stitches behind his right ear. On June 25, he returned to the starting linup after missing seven games. He logged the first three triples of his career. He connected for his second career grand slam in the first inning on May 14 against the Texas Rangers. He had a .997 fielding percentage and was ranked fourth among AL first basemen (3 E/1049 TC) in the 2007 season. He hit a solo home run on Opening Night against the Texas Rangers in his first at-bat of the season, his first home run since May 4, 2006, against the Detroit Tigers. He connected for the game-winning RBI single in the 9th inning on July 13 against the Rangers. He collected a career-best four hits in 1 game on August 17 against the Boston Red Sox.
On July 29, 2008, Kotchman was traded to the Atlanta Braves along with minor league pitcher Stephen Marek for first baseman Mark Teixeira.[1] Kotchman started his tenure with the Braves on a terrible note, batting just .157 in his first 20 games but he ended the season with a .237 batting average with the Braves.[2]
Kotchman was placed on the bereavement list after his mother fell ill on August 20, 2008.[3] After seven days, the Braves placed Kotchman on the restricted list so he could continue to be with his mother in Florida.[4] After 11 days from first being placed on the bereavement list, the Braves activated Kotchman.[5]
On January 31, 2009, Kotchman avoided arbitration and agreed to a one-year, 2.885 million contract.[6] Kotchman mentioned at spring training in 2009 that playing for the Braves was always a dream of his.[7] Kotchman suffered a shin contusion after being hit by a pitch from Arizona Diamondbacks' pitcher Max Scherzer on May 31 and was expected to be out two to three days.[8] The injury did, however, force Kotchman on the 15-day disabled list and was activated on June 16.[9]
On July 31, 2009, Kotchman was acquired by the Red Sox in a trade for first baseman Adam LaRoche.[10] In Kotchman's first game with the Red Sox, he hit a two-run home run off of Joba Chamberlain.[11] With the Red Sox, Kotchman hit .218 with three doubles, one home run, seven RBIs, and one stolen base in 29 games. This gave Kotchman a combined average of .268 with 23 doubles, seven home runs, 48 RBIs, and 39 walks in 126 games between Boston and Atlanta. Kotchman did not make an error in 114 games at first base for the Braves and Red Sox in 2009, becoming the third first baseman in major league history with a minimum of 108 games at the position in a season to record a perfect fielding percentage.[12]
In early January 2010 the Red Sox traded him to the Seattle Mariners for utility player Bill Hall, a minor league player, and cash.[13][14][15] On February 3, Kotchman and the Mariners agreed on a new contract, avoiding salary arbitration.[16]
On June 3, 2010, Kotchman set the Major League Baseball individual streak record with 2,003 consecutive chances without an error. The previous record had been held by Kevin Youkilis of the Boston Red Sox, established between July 4, 2006, and June 6, 2008.[17] On August 21, 2010, the streak ended when Kotchman mishandled a hard groundball hit by Curtis Granderson of the New York Yankees. The error was his first since July 20, 2008, when he played for the Los Angeles Angels of Anaheim, a streak of 2,379 fielding chances.[18]
He batted .217 for the season.[19] On November 4, 2010, Kotchman refused an outright assignment to AAA by the Mariners, electing to become a free agent instead.[20]
Kotchman signed a minor league contract with an invitation to 2011 spring training with the Tampa Bay Rays.[21] He was one of three players competing for two spots on the bench, with the other two being outfielder Sam Fuld and infielder Elliot Johnson.[22] On March 26, however, the Rays assigned Kotchman to Durham.[23] Following the retirement of Manny Ramirez six games into the 2011 season, Kotchman's contract was selected from Durham and he was added to the Rays' active roster. On August 9, 2011, Kotchman hit a walk-off home run off Kansas City Royals reliever Blake Wood to give the Rays a 2-1 win.[24] Through 2011, he had the best career fielding percentage among major league first basemen (.998), ahead of Kevin Youkilis.[25]
Kotchman signed a one year contract with the Cleveland Indians on February 3, 2012.[26]
He is the son of Tom Kotchman, longtime Angels' minor league manager and scout. His mother, Sue Kotchman, works as a principal and was formerly a teacher.[27] She suffered a brain hemorrhage during the 2008 season; as a result Kotchman had to be placed on the Major League bereavement list, and later the restricted list.[27] She recovered, however.[27] His sister Christal Kotchman is a former College of Charleston softball team member.[27]
|Wikimedia Commons has media related to: Casey Kotchman
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Ryan Williams has not received any gifts yet
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Happy Birthday Ryan! Regards, Paul
I have many interests. Which do you have in mind?
thanks for adding me as a friend. I'm looking at doing a pilot impression. I think that would be cool to do


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We all crave a bowl of steaming soup every now and then. Soups are healthy and comforting, and they don't always have to be straight out of a tin or packet. Wouldn't it be fun to make your own
steaming bowl from scratch! No added preservatives, flavour or colour, just natural ingredients and a recipe in hand! Home-made soups don't cost much, are a lot of fun to make, and a healthy
choice, when you're craving a snack. A clear soup will cleanse your palate or set the tone before a fine dining experience. However, soups are no longer just a prelude to a meal. They can easily
be the main event – a thick and chunky soup, with a piece of crusty bread on the side, makes for a truly filling meal. Stir up a quick soup on the go, or put on a pot and let it simmer till liquid gold.
Throw in some leftovers – surprisingly good additions to soups - and you have a bowl of contentment that will leave you with a warm afterglow. Find out how, with my selection of
Indian and international soups. Sip on the light and refreshing Clear Lemon and Coriander Soup; slurp on delicious Creamed Red Pumpkin and Apple Soup; indulge on the creamy Mushroom Soup with Almonds; or guzzle a bowl of rustic Spinach and Rice Soup and treat your senses to some delicious soups!
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Claims About Flood-damaged Cars Aren't True
A spokeswoman for Experian, which runs a Carfax competitor called AutoCheck, said the area that got blasted by Sandy has 9 million registered cars, far more than in the Gulf region struck by Katrina.
On Wednesday, the National Automobile Dealers Association put out a statement estimating that 200,000 or more flooded cars could be resold as used.
The trade organization warned that the storm could crimp the supply of clean used cars, potentially driving up prices. But its estimate was based on reports from third parties that showed 600,000 cars were damaged in Katrina and that Sandy would cause about one-third of the dollar damage from Katrina.
"There was not an incredible amount of science behind it," conceded Jonathan Banks, executive automotive analyst with the NADA Used Car Guide.Katrina overwhelmed low-lying areas of the Gulf Coast, including New Orleans, which is below sea level, causing widespread flooding. Many people in Katrina's path didn't evacuate, and car dealers didn't have many options to protect inventories. In hard-hit New Jersey, flooding from Sandy was mainly confined to a strip along the coast from Atlantic City to New York, and most people evacuated those areas, Appleton said. In the western part of the state, "unless a tree fell on your car, your car wasn't even at risk," he said. Chris Basso, another Carfax spokesman, said the company relied on an estimate from a trusted industry source in putting out its news release. He said it was just an estimate, and the final number won't be known for a while. "All we're trying to do is make sure people are looking out for these cars," he said. "Because eventually they're going to make their way back onto the road." Regardless of the predictions, thousands of flood-damaged cars will certainly be resold, and buyers need to be cautious.
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"The recipe was awesome&except for the glaze which was unnecessary. Before glazing, the skin was crackly and delicious and the meat had a wonderful flavor from the brine. After glazing, the skin was rubbery and gummy and unappetizing, and the glaze flavor overwhelmed the beautiful subtle flavors of the pork. Would definitely try this again without ""


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http://www.w3.org/Bugs/Public/show_bug.cgi?id=2155 email@example.com changed: What |Removed |Added ---------------------------------------------------------------------------- Status|NEW |RESOLVED Resolution| |FIXED ------- Additional Comments From email@example.com 2005-09-14 14:37 ------- Discussed at the Sept. 13 telecon. The WG agreed with HT on the proposal and is instructed to draft erratum text. Draft errata posted at: http://lists.w3.org/Archives/Member/w3c-xml-schema-ig/2002Oct/0201.html Discussed and approved at Oct. f2f. See: http://www.w3.org/XML/2002/10/xml-schema-ftf-minutes Erratm E1-38 added.Received on Wednesday, 14 September 2005 14:37:42 GMT
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|Samsung Electronics Sues Apple Over Patents
|Written by Editor
|Saturday, 23 April 2011 09:38
Seoul, April 22 (QNA) - Samsung Electronics Co., the world's second-largest maker of mobile phones, said Friday it filed lawsuits against Apple Inc. in South Korea, Japan and Germany, accusing the U.S.-based company of violating its patents in producing the iPhone and the iPad.The move comes after Samsung vowed to respond firmly to Apple''s allegation that Samsung''s Android-based mobile devices copied trademarks, trade dress and other patents of the iPhone and iPad. The federal case filed on Friday in San Jose district court claims the South Korean manufacturer violated 10 iPhone and iPad-related patents in Samsung''s Android-based smartphones and Galaxy Tab, the first serious iPad competitor to emerge late last year."Instead of pursuing independent product development, Samsung has chosen to slavishly copy Apple''s innovative technology, distinctive user interfaces, and elegant and distinctive product and packaging design," the suit argues. Apple is already a party to numerous lawsuits over smartphone functions, where it has a disadvantage because of a smaller patent portfolio and shorter history in the industry than some of its rivals.The new suit is the first against Samsung, whose corporate affiliates supply Apple with components. Unlike most of the phone litigation, Apple''s new case stresses "trade dress", or the appearance of the products, and it includes photographs showing a marked similarity in the tablets'' shape and even the appearance of the onscreen icons, which are uniform squares with rounded corners.While those claims might have a commonsense appeal and avoid the complexity of patent issues, the burden to win is heavier. Aggrieved companies typically win only if they can show that consumers will be so confused by the copying that they won''t realise who the manufacturer is. "Samsung will respond actively to this legal action taken against us through appropriate legal measures to protect our intellectual property," the company said, suggesting it could counterclaim that Apple violated Samsung patents.
By: QNA


text:
I will wear White Forgiveness
It was long before dawn on a winters night when I first found the warmth of White Forgiveness,
and like the arms of a lover I stretched out my arms and allowed it to embrace in me that which hands have never held.
(I wanted nothing more) From heaven fell Gods crystallized tears, covering me with White forgiveness and allowing me to melt in the beauty now surrounding me. ( I needed nothing more).
Oh, the joy! Born of my desire to be covered with the hope of White forgiveness... as I watched from heaven Him coming to claim (what yesterday), had been a vision of crime,- and for a moment, (maybe two) I allowed myself the hope I found In its forgiveness and my heart ached at knowing love of Him again. (I wanted nothing more).
Oh, White Forgiveness! You blanket my darkness with the freshness of White and reflect the sun -So bright -that it can not be ignored, …any more.
Yes, long before dawn on a winters night i stood at my window and listened for the approach of White Forgiveness, coming to heal me from surrealistic photos so full in my mind, and cluttering up my senses, - causing me to stumble in my search for self and start new. I waited, and Forgiveness came. (and when it beckoned me, it was to My Freedom that I ran,... feeling for the first time the threadbare gauze of sanity (wrapped so jealously around me) fall away -and in its place White Forgiveness covered me.
Catherine Mayrand
you have a beautiful voice
you have a beautiful voice and a powerful way with words!
Very Lovely!
I enjoyed reading your story, it was almost like poetry! Very nicely written!
"I embrace emerging experience. I am a butterfly. Not a butterfly collector." - Stafford


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Kwan K., Cooper M., La Duc M.T., Vaishampayan P., Stam C., Benardini J.N., Scalzi G., Moissl-Eichinger C. and Venkateswaran K.
Notes: These authors used the Maxwell® 16 System to extract DNA from multiple sample collection devices containing a model microbial community (MMC) comprised of 11 distinct species of bacterial, archaeal and fungal lineages associated with spacecraft or clean-room surfaces. The authors compared cotton swabs, polyester wipes and biological sampling kits to assess the success of recovering DNA of rRNA genes for species-specific PCR analysis. (4124)
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Maxwell® 16 Cell DNA Purification Kit
Maxwell® 16 Cell LEV DNA Purification Kit
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dos Santos, F.B., Nogueira, F.B., Castro, M.G., Nunes, P.C., de Filippis, A.M., Fariam, N.R., Simões, J.B., Sampaio, S.A., Santos, C.R. and Nogueira, R.M.
Notes: The authors examined the strains of Dengue virus serotype 1 (DENV-1) found in the State of Rio de Janeiro since it was introduced in 1986. Viral RNA was extracted from patient serum samples or cell culture and 5µl of RNA reverse transcribed and amplified using the AccessQuick™ RT-PCR System with primers for the 2,325bp C/prM/M/E region of DENV-1. The products were sequenced and aligned to examine how the virus had evolved over time. (4342)
AccessQuick™ RT-PCR System
Ismail, N.A., Ragab, S.H., Elbaky, A.A, Shoeib, A.R., Alhosary, Y., and Fekry, D.
Notes: These authors investigated the differences in gut microbial flora between obese and normal-weight subjects. They used the Wizard® Genomic DNA Purification Kit to extract DNA from diluted fecal extracts. The extracted DNA was analyzed by PCR to identify Bacteroidetes and Firmicutes. Differences in distribution of these phyla was between the subject groups were identified. (4220)
Wizard® Genomic DNA Purification Kit
GoTaq® Flexi DNA Polymerase
Westphal, A., Williams, M.L., Baysal-Gurel, F., LeJeune, J.T. and McSpadden Gardener, B.B.
Notes: The authors investigated the suppression of E. coli O157:H7 in sand-based livestock bedding and hypothesized that suppression of E. coli O157:H7 growth was mediated by an environmentally stable population of pathogen-suppressing bacteria. These bacteria were identified by terminal restriction fragment length polymorphism (T-RFLP) analysis of amplified 16S rRNA gene sequences isolated from used bedding followed by cloning and sequencing of the most abundant terminal restriction fragments. Amplifications were performed using the GoTaq® Flexi DNA Polymerase, then PCR products were cloned into the pGEM®-T Easy Vector. The PureYield™ Plasmid Miniprep System was used to purify plasmids for sequencing. (4165)
pGEM®-T Easy Vector System II
Crawford, M.A., Lowe, D.E., Fisher, D.J., Stibitz, S., Plaut, R.D., Beaber, J.W., Zemansky, J., Mehrad, B., Glomski, I.J., Strieter, R.M. and Hughes, M.A.
Notes: The authors identified three genetic loci involved in chemokine-mediated antimicrobial effects against Bacillus anthracis using a transposon mutant library in which a transposon is randomly inserted into the B. anthracis genome, then treating the mutant cells with chemokine to select for resistant cells. To identify the transposon insertion site, and thus the resistance-conferring loci, the authors amplified regions flanking the transposon by PCR using the GoTaq® Green Master Mix. (4167)
GoTaq® Green Master Mix
Racca A.C., Camolotto S.A., Ridano M.E., Bocco J.L., Genti-Raimondi S., and Panzetta-Dutari, G.M.
Notes: These authors studied KLF6 expression during human trophoblast cell differentiation, and its role in the regulation of genes associated with placental development and pregnancy maintenance. They used immunofluorescence microscopy, RT-qPCR and luciferase reporter assays to investigate cellular localization, mRNA expression, and transcriptional activation. Reporter assays were performed using various luciferase reporter constructs, the Dual-Luciferase® Assay, and the GloMax®-Multi Detection System. KLF6 was shown to play a role as transcriptional regulator of relevant genes for placental differentiation and physiology such as βhCG and PSG. (4197)
pGL3-Basic Vector
Dual-Luciferase® Reporter Assay System
Passive Lysis 5X Buffer
GloMax®-Multi Base Instrument
Yusof, M.A., Rais, F., Abdullah, M.A., Zamri, L.A., Ali, H.M., Kassim, F.M. and Saat, Z.
Notes: This study characterized the strains of hand, foot and mouth disease (HFMD) that circulated in Peninsular Malaysia and Sabah during the last ten years. Viral RNA was extracted from vesicle, throat and rectal swabs, and 5µl of purified RNA was amplified in a 50µl reaction using the AccessQuick™ RT-PCT System with primers for VP4 or VP1. The amplified products were then sequenced and compared to known HFMD sequences. (4341)
Goldberg, M.S., Xing, D., Ren, Y., Orsulic, S., Bhatia, S.N., and Sharp, P.A.
Notes: These authors evaluated the effect of knockdown of Parp1 on survival of tumor cells after in vivo delivery of anti-Parp siRNA in a lipid-based nanoparticle format. Inhibition of Parp1 expression in mouse tumors was confirmed by qPCR analysis. Total RNA was extracted from the tumors using TRIzol® reagent (Life Technologies) and reverse transcribed using the ImProm-II™ Reverse Transcription System prior to qPCR using SYBR Green PCR Master Mix (Applied Biosystems) . (4222)
ImProm-II™ Reverse Transcription System
Fuehrer, H.P., Fally, M.A., Habler, V.E., Starzengruber, P., Swoboda, P. and Noedl, H.
Notes: The authors developed a direct-amplification, nested PCR protocol to amplify Plasmodium DNA from 903 filter paper punches containing whole blood. The second round of nested PCR was performed using 2.5µl of PCR products from the first round and GoTaq® PCR Core System. Parallel PCRs were performed using purified DNA from blood, and both rounds of nested PCR were performed using the GoTaq® PCR Core System. (4166)
GoTaq® PCR Core System I
Jheon, A.H., Mostowfi, P., Snead, M.L., Ihrie, R.A., Sone, E., Pramparo, T., Attardi, L.D. and Klein, O.D.
Notes: The authors determined that PERP, a tetraspan membrane protein, is required for enamel formation during tooth development in mice. Using microarray analysis, they then identified genes that were differentially expressed in wildtype and Perp-null mice and might be involved in enamel formation. Differential expression of these genes was confirmed by qPCR using the GoTaq® qPCR Master Mix. The authors also identified an upstream regulator of Perp, P63, by transfecting cells derived from embryonic mouse teeth with a Perp-luciferase reporter construct that contained a P63 response element or mutated P63 response element. A Renilla luciferase vector was used for normalization of transfection efficiency, and luciferase assays were performed using the Dual-Luciferase® Reporter Assay System. (4168)
GoTaq® qPCR Master Mix
Zhelyazkhova, P., Hammani, K., Rojas, M., Voelker, R., Vargas-Suárez, M., Börner, T., and Barkan, A.
Notes: Pentatricopeptide repeat (PPR) proteins are helical repeat proteins that bind specific RNA segments and protect the adjacent RNA by serving as a barrier to exoribonucleases. This study showed that protection by PPR or PPR-like proteins is the predominant mechanism for defining the positions of processed 5′ and intercistronic mRNA termini in land plant chloroplasts. The authors used RNasin® Ribonuclease Inhibitor in binding reactions between labeled RNA and PPR proteins prior to Gel mobility shift assays. They also used the pGEM®-T Vector to clone various 3´ RNA terminal sequences amplified by RT-PCR. (4185)
pGEM®-T Vector System I
pGEM®-T Vector System II
RNasin® Ribonuclease Inhibitor
Dai, W., Zhang, G. and Makeyev, E.V.
Notes: The full-length 3´ UTR of mouse RNA-binding protein HuR was amplified and cloned into psiCHECK™-1 Vector to create pEM429 plasmid. To generate radiolabeled RNA substrates for use in cleavage assays, RNA was synthesized from 1µg of linearized plasmid using 50µCi of [α-32P]UTP, 0.8mM Ribo m7G Cap Analog and T7 RNA Polymerase by incubating for 1.5 hours at 37°C. The RNAs were then treated with 1 unit of RQ1 RNase-Free DNase per 1µg of template DNA for 15 minutes at 37°C before extracting with phenol:chloroform, precipitating with ethanol and resuspending in DEPC-treated water. The cleavage assay used 60fmol of 32P-labeled substrate RNA with 10U Recombinant RNAsin Ribonuclease Inhibitor in a reaction incubated for 2.5 hours at 30°C. RNA probes were labeled with biotin using T7 or T3 RNA Polymerases with a biotin-UTP labeling NTP mixture and incubated for 2 hours at 37°C. The biotinylation reaction was then treated with RQ1 RNase-Free DNase following the same protocol used for radiolabeled RNA. To form HuR/RNA complexes, 2µg of biotinylated RNA was mixed with 100µg nuclear extract and 40 units Recombinant RNAsin® Ribonuclease Inhibitor in a total volume of 20µl, and incubated for 30 minutes at room temperature. For CstF-64/RNA complexes, 1µg of biotinylated RNA was used and the complexes were stabilized by UV crosslinking using 10U Recombinant RNAsin Ribonuclease Inhibitor during the UV treatment. NIH 3T3 cells were UV crosslinked and either total or nuclear RNA used for immunoprecipitation. After extraction the RNAs were treated with RQ1 RNase-Free DNase for 15 minutes at 37°C before RT-PCR using HuR-specific primers. Total RNA purified from cultured cells were incubated with 50U/ml RQ1 RNase-Free DNase at 37°C for 30 minutes before use in RT-qPCR. (4187)
psiCHECK™-1 Vector
Recombinant RNasin® Ribonuclease Inhibitor
Ribo m7G Cap Analog
RQ1 RNase-Free DNase
T7 RNA Polymerase
Li, Z., Li, D., Du, X., Wang, H., Larroque, O., Jenkins, C.L., Jobling, S.A. and Morell, M.K.
Notes: The authors investigated starch synthesis in barley (Hordeum vulgare) by examining mutations in class I, class II and class III starch synthases (ssI, ssII and ssIII, respectively). Mutations of ssIIa and ssIIIa were detected by PCR using the GoTaq® Hot Start Polymerase. (4162)
GoTaq® Hot Start Polymerase
Maruyama, A., Nishikawa, K., Kawatani, Y., Mimura, J., Hosoya, T., Harada, N., Yamamato, M. and Itoh, K.
Notes: These authors first used a FLAG-tagged protein (nfr2) with a HeLa Nuclear extract and captured interacting proteins via SDS-PAGE and in-gel digests of bands to identify (Krüppel-associated box)-associated protein 1 (KAP1) as a potential interacting partner. Human KAP1 was purchased as a HaloTag® CMV Flexi® Vector from Kazusa and used in a Mammalian PullDown scenario (with HaloLink™ Resin) to demonstrate interaction between the two proteins. A reporter assay was used to show that KAP1 facilitates Nrf2 transactivation in a dose-dependent manner. The authors defined the interaction sites using GST-tagged nrf2 and various forms of KAP1-HaloTag® Fusions expressed in TNT® SP6 High-Yield Wheat Germ Extract. GST-tagged proteins were expressed in E. coli and bound to glutathione-Sepharose beads. These bound proteins were mixed with the KAP1 from the cell-free expression system, incubated for 4 hours at 4°C, washed and stained with the HaloTag® TMR Ligand for 30 minutes. The proteins from the pull-down assay were subjected to SDS-PAGE and the HaloTag® proteins detected by phosphorimaging and the GST proteins by Coomassie Brilliant Blue Staining. A two-hybrid system consisting of the pRL-TK Vector with a firefly luciferase reporter with Gal4 UAS, mouse Nrf-2 N-terminal domain and KAP1 was also used. The vectors were transfected into Nrf2 knockout MEFs for 4 hours then incubated for 36 hours before luciferase expression was determined using the Dual-Luciferase® Reporter Assay System. (4123)
Anti-HaloTag® pAb
HaloTag® TMR Ligand
pFN21A HaloTag® CMV Flexi® Vector
pGEM®-T Easy Vector System I
pRL-TK Vector
Wanaratana, S., Panyim, S. and Pakpinyo, S.
Notes: To investigate if house flies could act as a vector for avian influenza virus H1N5, flies exposed to the virus were homogenated and the homogenate used to inoculate 10-day-old embryonated chicken eggs (ECEs). Allantoic fluids were collected from the eggs, RNA purified from the samples and the presence of H1N5 assessed using M-specific primers and the AccessQuick™ RT-PCR System and looking for the 276bp amplimer. (4339)
Nocchi, L., Tomasetti, M., Amati, M., Neuzil, J., Santarelli, L. and Saccucci, F.
Notes: Thrombomodulin (TM) expression was examined by isolating genomic DNA from biopies of human malignant mesothelioma and normal mesothelial tissue, and cultured cell lines with or without PARP1 silencing treated with 5-aza-2´-deoxycytidine and trichostatin alone or in combination and then subjected to biosulfide modification. To analyze methylation of TM, a CpG island in the promoter, 5´ UTR and an exon region containing 44 CpG dinucleotides were PCR amplified, cloned into the pGEM®-T Easy Vector, transformed and positive clones selected using IPTG/X-Gal and analyzed by PCR. Colonies were cultured, the plasmids isolated using the Wizard® Plus SV Minipreps DNA Purification System then 10 clonesfrom each sample type were sequenced. (4132)
Wizard® Plus SV Minipreps DNA Purification System
Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters
Mochida, S., Tsuzuki, S., Inouye, K. and Fushiki, T.
Notes: The authors determined that a recombinant catalytic domain of rat matriptase (His6t-S-CD) caused detachment of small-intestinal epithelial cells (IEC-6 cells) from laminin-coated plates. His6t-S-CD was expressed in the yeast P. pastoris and purified by ammonium sulfate precipitation, gel filtration through a PD-10 column, then Ni2+-chelating chromatography using HisLink™ Resin. The authors also treated IEC-6 cells with purified His6t-S-CD to determine if this domain induced apoptosis by monitoring annexin-V staining, DNA fragmentation and caspase-3 activity. For the DNA fragmentation analysis, IEC-6 cells were treated with His6t-S-CD, then harvested, and genomic DNA was purified using the Wizard® SV Gel and PCR Clean-Up System. DNA fragmentation was assessed by agarose gel electrophoresis and ethidium bromide staining. (4100)
HisLink™ Protein Purification Resin
Wizard® SV Gel and PCR Clean-Up System
Wynendaele, J., Böhnke, A., Leucci, E., Nielsen, S.J., Lambertz, I., Hammer, S., Sbrzesny, N., Kubitza, D., Wolf, A., Gradhand, E., Balschun, K., Braicu, I., Sehouli, J., Darb-Esfahani, S., Denkert, C., Thomssen, C., Hauptmann, S., Lund, A., Marine, J.C. and Bartel, F.
Notes: The authors identified a single nucleotide polymorphism (SNP) in the 3´ untranslated region (3´ UTR) of MDM4, which promotes tumorigenesis by decreasing p53 tumor suppressor function, in ovarian cancer cells. This A to C transversion creates a putative target site for the hsa-miR-191 microRNA in the MDM4-C allele, but not the wildtype MDM4-A allele. To determine if this SNP affects MDM4 translation efficiency or mRNA stability, the authors cloned a 224-bp fragment of the MDM4 3´UTRs into the psiCHECK™-2 Vector and transfected the ovarian cancer cell line A2780 with the 224A or 224C variants of the MDM4 3´ UTR. The authors used the luciferase-based psiCHECK™-2 Vector and Dual-Glo® Luciferase Assay System to show that the C variant dramatically reduces translation efficiency and/or mRNA stability. They also assessed MDM4 expression levels in A/A, A/C and C/C ovarian cancer cells and tissues using RT-qPCR. qPCR primers for MDM4 were designed using the Plexor® Primer Design Software, and assays were performed using the Plexor® qPCR System. (4157)
Dual-Glo® Luciferase Assay System
Plexor® qPCR System
psiCHECK™-2 Vector
Ruhlmann, J.M., Kram, B.W. and Carter C.J.
Notes: The authors used microarray analysis to identify genes that are differentially expressed in nectaries of Arabidopsis and may be involved in nectar synthesis and secretion. One of these genes was cell wall invertase 4 (cwinv4). The authors generated two Arabidopsis cwinv4 mutant lines to study gene function and used GoTaq® Green Master Mix to confirm the mutant genotype. Expression patterns of cwinv4 in wildtype Arabidopsis and an ortholog from Brassica rapa were examined in various tissues by RT-PCR. The reverse transcription step was performed using the Reverse Transcription System and 0.1µg of RNA. (4093)
Reverse Transcription System
Newell, D.L. and Morris, A.B.
Notes: The authors investigated genetic diversity in a Florida population of Illicium parviflorum, an endangered evergreen shrub, by amplifying intersimple sequence repeats (ISSRs). Amplifications were performed using GoTaq® Hot Start Polymerase. (4161)
Ciobanu, D.C., Lu, L., Mozhui, K., Wang, X., Jagalur, M., Morris, J.A., Taylor, W.L., Dietz, K., Simon, P. and Williams, R.W.
Notes: Sequence variation within a gene, such as single nucleotide polymorphisms (SNPs), can lead to differences in expressions levels of corresponding mRNAs; genes that are self-regulated by this mechanism (cis modulation) are difficult to identify accurately with existing techniques. The authors used bioinformatic and molecular approaches to estimate error rates when identifying cis-modulated transcripts and developed a simple method to detect these transcripts in C57BL/6J F1 hybrid mice. This method, which they named allelic specific expression, is RT-PCR-based and uses PCR primers that flank an informative SNP to quantify the differential expression levels of transcripts. GoTaq® Flexi DNA Polymerase was used in the PCR. (4051)
de Maat, M.F., Narita, N., Benard, A., Yoshimura, T., Kuo, C., Tollenaar, R.A., de Miranda, N.F., Turner, R.R., van de Velde, C.J., Morreau, H. and Hoon, D.S.
Notes: The authors examined the methylation status of methylated-in-tumor (MINT) loci and the degree of microsatellite instability (MSI) in colorectal cancer to determine if methylation of MINT loci during the progression of adenoma to cancer was linked to MSI. They used on-slide sodium bisulfite modification and methylation-specific PCR to examine the methylation index in paraffin-embedded tissue blocks containing normal, adenoma and cancer tissues. MSI status was determined using the MSI Analysis System. Patients with instability at more than 4 markers were classified as MSI-high, and patients with instability at more than 1 marker were considered microsatellite-stable. (4106)
MSI Analysis System, Version 1.2
Maier, R.H., Maier, C.J., Rid, R., Hintner, H., Bauer, J.W. and Onder, K.
Notes: The authors developed a high-density protein array using a recombinant peptide library to map the epitope recognized by a commercially available anti-vitamin D receptor (VDR) monoclonal antibody. By screening 2304 overlapping VDR peptides, they were able to identify the 37-amino-acid epitope. The library was created by amplifying the 1.2kb VDR coding region, cleaning the PCR product with the Wizard® SV Gel and PCR Clean-Up System, sonicating the PCR product, then cloning the VDR fragments into a bacterial expression vector that confers a glutathione-S-transferase (GST) tag. The epitope was verified by showing that the 37-amino-acid sequence was recognized in Western blot analysis and enzyme-linked immunosorbent assay (ELISA); the full-length VDR, also expressed as GST fusion protein, was used as a positive control. These GST fusion proteins were purified using the MagneGST™ Protein Purification System. (4154)
MagneGST™ Protein Purification System
Nishino, S.F., Shin, K.A., Payne, R.B. and Spain, J.C.
Notes: The authors identified a bacterial strain that can use the toxin 3-nitropropionic acid (3NPA) as its sole carbon and nitrogen sources and cloned the genes that encode the enzymes responsible for the initial steps in the 3NPA degradation pathway. The Pseudomonas library used to clone these genes was created using genomic DNA isolated using the Wizard® SV Genomic DNA Purification System. Closely related genes were amplified from other bacterial species for phylogenetic analysis. PCRs were performed using the GoTaq® Hot Start Polymerase. (4164)
Wizard® SV Genomic DNA Purification System
Diosdado, B., Buffart, T.E., Watkins, R., Carvalho, B., Ylstra, B., Tijssen, M., Bolijn, A.S., Lewis, F., Maude, K., Verbeke, C., Nagtegaal, I.D., Grabsch, H., Mulder, C.J., Quirke, P., Howdle, P. and Meijer, G.A.
Notes: To better examine the molecular mechanisms of small bowel adenocarcinomas, DNA was extracted from paraffin-embedded tissue and tested for microsatellite instability (MSI) using the MSI Analysis System, Version 1.2. The PCR products were run on the Applied Biosystems 3130 Genetic Analyzer and analyzed using GeneScan® software. If two or more of the the BAT-25, BAT-26, NR-21, NR-24 or MONO-27 monomorphic markers had altered lengths, the tumors were designated as MSI unstable. (4112)
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AFC Championship: Baltimore Ravens Vs. New England Patriots
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New England Patriots quarterback Tom Brady and New England Patriots... Nyhetsfoto 159839218Fjärde kvarten - Sport,Fotbollsreceiver,Foxborough - Massachusetts,Horisontell,Koppling,Massachusetts,NFL,New England Patriots,Passa,Quarterback,Sport,Tom Brady,USA,Wes WelkerPhotographer Boston GlobeCollection: Boston Globe 2013 - The Boston GlobeFOXBOROUGH, MA - JANUARY 20: New England Patriots quarterback Tom Brady (#12) and New England Patriots wide receiver Wes Welker (#83), at left, couldn't connect on a pass late in the fourth quarter. There are questions about whether Welker will be around for Brady next season. The New England Patriots hosted the Baltimore Ravens in the AFC Championship Game at Gillette Stadium. (Photo by Barry Chin/The Boston Globe via Getty Images)


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40718 results for "high school"
Arizona State Sun Devils guard Shaquielle McKissic's journey to D...Dana O'Neil
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ALLSTON, Mass. -- St. Sebastian's was just too much for Catholic Memorial on Tuesday night at Harvard's Bright Landry Rink. The Arrows capitalized on three plays in transition and were stingy in their own end as they shut out the Knights, 3-0, to ...Blog | December 24, 2014
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Rakeem Cato capped a record-setting career by tying an NCAA mark for touchdown passes and throwing for three scores to help Marshall beat Northern Illinois 52-23 Tuesday night in the inaugural Boca Raton Bowl.Recap | Box Score | Conversation | December 23, 2014
McLeod, Dottin lead dynamic Cambridge backcourtNate Weitzer
CAMBRIDGE, Mass. -- The scoreboard in Bedford High School's gymnasium read 16 seconds. Sophomore guard Jakigh Dottin skied for a defensive rebound, pivoted immediately after hitting the ground, and burst down the floor. He split two defenders with...Blog | December 23, 2014
Recruit literally bathing in recruiting lettersStaff
How wanted is top football recruit Quart'e Sapp? Wanted enough to fill a bathtub full of letters from schools interested in him.Story | Conversation | December 23, 2014
Recruiting Tales: Detroit Lions RB Theo RiddickMichael Rothstein
Before guys made the NFL and before they became consistent starters or better in college, someone found them in high school. With that in mind, we're starting a series called Recruiting Tales, where we chat with the main college recruiter of a Detroi...Blog | December 23, 2014
Our preseason MIAA girls' hockey All-State teamESPNBoston.com
Presenting our MIAA girls' hockey preseason All-State team: 1st Team F - Emily Loprete, Sr., Watertown/Belmont F - Ali O'Leary, Jr., Reading F - Adrieana Rossini, Sr., Arlington Catholic D - Jaimee Cooke, Jr., Austin Prep D - Caroline Seibold...Blog | December 23, 2014
Greater Northeast Girls Basketball ShowcaseBrendan C. Hall
Braintree High School is the epicenter for top-flight high school girls basketball in New England this holiday weekend, as some of the top programs from the northeast are squaring off in the first annual Greater Northeast Girls Basketball Showcase. ...Blog | December 23, 2014
BABC Holiday Classic scheduleBrendan C. Hall
A number of teams currently ranked in ESPNBoston.com's statewide Top 25 will be squaring off this coming weekend in the Boston Amateur Basketball Club's annual BABC Holiday Classic. The three-day showcase event will take place Saturday, Sunday and Mo...Blog | December 23, 2014
Five observations: City of Palms ClassicReggie Rankin
FORT MYERS, Fla. --- The 42nd Culligan City of Palms Classic High School Basketball Tournament brought some of the nation's top talent and teams to Southwest Florida for six days of action at Bishop Verot High School. 1. ESPN 100's No. 1 prospect ...Blog | December 23, 2014
Big 3 makes history in latest boys hoop pollBrendan C. Hall
We updated our statewide MIAA Top 25 boys basketball poll this afternoon. To view it, CLICK HERE. With many key holiday tournaments bleeding into the early part of the week, our next poll update will be on Tuesday, Dec. 30. Scores from tonight'...Blog | December 22, 2014
Hal Gill brings NHL pedigree to L-S staffJohn McGuirk
MARLBOROUGH, Mass. -- Hal Gill still yearns for a return to the NHL. Having spent the better part of 16 years in the league, Gill became a free agent after spending a cup of coffee with the Philadelphia Flyers last season. At 39 years of age, Gil...Blog | December 22, 2014
Reading to host Bauer Winter Classic ShowcaseScott Barboza
Reading Memorial High School will host the Winter Classic Showcase presented by Bauer, featuring the boys' hockey teams from Andover, Arlington Catholic, Billerica and Reading. Here's a look at the schedule, with all action scheduled for Readin...Blog | December 22, 2014
The Superfans Using Stats To Get To The Bottom Of 'Serial...
"" is over, but the podcast's fans aren't done debating who committed the 1999 murder of Baltimore County high school student Hae Min Lee. And statisticians have ideas about how to make the debate smarter. "Serial&...Blog | December 22, 2014
Utah poised to move up in Pac-12 pecking orderTed Miller
In the 1985 high school wrestling movie "Vision Quest," Louden Swain is possessed by the idea of taking on fearsome undefeated state champion Brian Shute. In fact, he shows up during one of Shute's personal workouts -- he's carrying a huge log up and...Blog | December 22, 2014
Recap: Salisbury takes 2nd straight Flood-Marr titleMike Abelson
DEDHAM, Mass. - The Salisbury School put up a pair of goals in the third period to skate by The Westminster School, 3-1, to win the Flood-Marr tournament for the second straight year. It was the Crimson Knights' third title in six years overall. ...Blog | December 21, 2014