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Book your room at Lamai Bay View Resort
Standard Bungalow, Sea ViewMax Occupancy: 2 guests (up to 1 child, 1 infant)
Superior Bungalow, Sea ViewMax Occupancy: 2 guests (up to 1 child, 1 infant)
Deluxe Bungalow, BeachsideMax Occupancy: 2 guests (up to 1 child, 1 infant)
Standard Bungalow FanMax Occupancy: 2 guests (up to 2 children, 2 infants)
3-star resort near the beach
This Koh Samui resort offers direct access to the beach and is only a short distance from Silver Beach, Coco Splash Waterpark, and Lamai Night Market. Local attractions also include Hin Ta and Hin Yai and Wat Lamai.
Resort Features
At Lamai Bay View Resort, relax by an outdoor pool or hit the nearby beach where you can enjoy kayaking.
Room Amenities
Televisions come with cable channels. Guestrooms also feature complimentary wireless Internet access, refrigerators, and premium bedding.Information missing or incorrect? Tell us!
At your hotel
At your hotel
The resort offers a restaurant. A bar/lounge is on site where guests can unwind with a drink. High-speed wireless Internet access is complimentary. For a surcharge, the property provides a roundtrip airport shuttle (available on request), an area shuttle, and a ferry terminal shuttle. This beach resort also offers an outdoor pool, tour/ticket assistance, and barbecue grills. Concierge services, limo/town car service, and wedding services can be provided.
- Supervised childcare/activities (surcharge)
- Airport transportation (surcharge)
- Total number of rooms - 26
- Bar/lounge
- Breakfast available (surcharge)
- Complimentary newspapers in lobby
- Concierge services
- Dry cleaning/laundry service
- Front desk (limited hours)
- Ferry terminal shuttle (surcharge)
- Shopping center shuttle (surcharge)
- Free Wi-Fi
- Area shuttle (surcharge)
- Laundry facilities
- Garden
- Barbecue grill(s)
- Babysitting or childcare
- Limo or Town Car service available
- Multilingual staff
- Picnic area
- Luggage storage
- Wedding services
- Tours/ticket assistance
- Restaurant
- Outdoor pool
Stay connected
Available in all rooms: Free Wi-Fi
Available in some public areas: Free Wi-Fi
In your room
- Premium bedding
- Refrigerator
- Daily housekeeping
- Private bathroom
- Bathtub or shower
- Television
- Minibar
- Cable TV service
- Free Wi-Fi
- In-room massage available
- Room service (limited hours)
Eat and drink
Continental breakfasts are available for a surcharge and are served each morning between 6:30 AM and 10:30 AM.
Lalla Restaurant and Bar - This beachfront restaurant serves breakfast, lunch, and dinner. Guests can enjoy drinks at the bar.
Room service (during limited hours) is available.
The recreational activities listed below are available either on site or nearby; fees may apply.
- Kayaking on site
- Massages
The fine print for Lamai Bay View Resort
When you arrive
Check-in time starts at 2 PM
Check-in time ends at 10 PM
Minimum check-in age is 18
When you leave
Check-out time is noon
How you can pay
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
No pets or service animals allowed
We should mention
This property offers transfers from the ferry terminal and airport (surcharges may apply). Guests must contact the property with arrival details 72 hours prior to arrival, using the contact information on the booking confirmation. Reservations are required for massage services. Reservations can be made by contacting the resort prior to arrival, using the contact information on the booking confirmation. Only registered guests are allowed in the guestrooms. No pets and no service animals are allowed at this property.


text:
I'm the holder of these calls and manage QSL's for the following: LA9DFA - JW9DFA - LI9DFA - LB8DC - LA3C - LI3C - LN3C - JW3C.
QSL is OK via the buro, LoTW or direct for all calls. No eQSL.
I am a member of the Qsl Managers Society, see link or email me if you need a QSL-manager. http://www.qsl.net/qslmanagers
QSL information: I prefer direct requests, although I will answer buro cards as well. Direct requests require full return support or they will be returned via the buro. Please include a self-addressed envelope and sufficient return postage. For postage to EU and the rest of the world, 1$ dollar is not enough. Please provide 1IRC or 3$ for EU and the rest of the World. IRC's must be stamped in the "box" on the left side. Without this stamp the IRC's are useless, and the QSL will be returned through the bureau.
Previous DXSpeditions:
Hope to hear you in the pile-up!
Setup August 2013 includes:
Icom 7600 (HF+6m), Icom IC-756PROII (HF+6m), Yaesu FT-847 (HF+VHF+UHF)
Acom 1500 (HF+6m), SPE Expert 1K-FA (HF+6m), Sommerkamp SLA200 (200W on 144MHz), Mirage B2518 G, (150W on 144MHz)
Microham Microkeyer II
Optibeam OB11-5 on 10,12,15,17 and 20m, BigIr on 30 and 40 with elevated radials. 1/2 wave dipole on 60m up 15m. 1/2 wave dipole on 80m up 17m. Inv-L (3/8 wave on 160m).
7LFA6WOS on 50MHz, Eagle 4M6DX on 70MHz and 2M5WL on 144MHz.
If you hear me running a pileup, if possible please make a recording of it and send it to me on e-mail at the following address: la9dfa at online dot no. Many thanks!!
If you do I will reply to your recording with a direct QSL card.
I support the DX Code of Conduct.
Please visit this site and read it carefully!!
73's de Morten LA9DFA
Tower 1: 18 meter high, with OB11-5 for 10,12,15,17 and 20 meter at 18 meters hight. On top you can see a 7LFA6WOS, a 7 element LFA for 50MHZ at 21 meter.
Tower 2: 12 meter high, with a 2M5WL for 144 MHz. That's 17 elements on a 10 meter boom!! On top you can see a 4M6DX, a 6 element for 70 MHz at 15 meter hight.
1474760 Last modified: 2014-11-26 20:48:23, 4137 bytes
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Currently updating logbook display.

subset: commoncrawl


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- Home /
- AI Director Wireless Controller - Aqua Illumination
Aqua Illumination Vega Color LED Module
Starting at: $399.00
Aqua Illumination Vega Blue LED Module
Starting at: $399.00
Aqua Illumination Hydra LED Module
Starting at: $399.00
Horrible By Mikey on 12/11/2014Mikey wouldn't recommend this product to a friendThis product makes sense until it arrives in the mail. Then, it just does NOT work. Sorry, great idea, but horrible execution, clearly no QA/QC on this one.
Don't Listen to the Naysayers By Danny on 12/6/2014Danny would recommend this product to a friendI was skeptical of purchasing this product after all the negative reviews. I nearly didn't because of them. After receiving the controller I did all the updates and started messing with the spectrum. I had the lights for 3 months with only the ability to turn them on through their 6 preset modes and discovered with the controller that my white and royal blue spectrum were not functioning. It took a long time to complete the updates required but no longer than an update on a video game console. The best part is that when I reported my failed spectrum on the Hydras to AI, they sent me two new lights with pre paid return shipping no questions asked. I reported it on a Monday and had two new lights by Thursday. I will always buy in confidence with this company based on their customer service.
Stay Away By Thinker 23 on 11/17/2014Thinker 23 wouldn't recommend this product to a friendUnstable, nothing but problems. Stay away or be prepared for a frustrating experience
Wasn't thoroughly tested by AI By Great in theory on 5/5/2014Great in theory wouldn't recommend this product to a friendThe idea of this product is great... control your lights from your computer or phone. Except I think AI released the product without testing it appropriately. Since the day I purchased this product my lights have not worked properly. The director does not connect wirelessly (I have it connected to ethernet), the power is continuously lost which makes my light malfunction (they'll stay on all day or not turn on at all), updating the firmware continuously fails, I've had to reset my lights and director at least once a day since I bought it, I've spent days at a time without lights on my tank because I had to disconnect the power (or the lights would stay on all night). I could go on but the point is that this product was a waste of money.
All in all, if you're set on AI, I would go for the controller. Stay away from the director.
Not what it should be By Frank on 4/1/2014Frank wouldn't recommend this product to a friendI would not get this unit at this point, their are software issues. Your lights will not work right. But you need to wait for the large software update this could come out with in the next few weeks.
And then I don't know if this is the way to go at this point. But for the money this is what you get issue after issue as for calling AI they try to help you but the tech are tide as well. not right for this company to do this. And as for anyone who' s don't have the money to spend this is what you will get is issue. Not what I would have thought I deal with.
Takes time, but i now trust it to work By Alex on 3/11/2014Alex would recommend this product to a friendI was very disappointed when i purchased the director, and ended up using the other controller i had.
The next day, a new update was available fixing the bugs i was having, what a "coincidence"!
Now that i have set up the system, and contacted customer service after having minor problems that were my fault, everything now works.
I believe that it is best to factory reset ALL the lights you own BEFORE you hook them up to the director, then update the firmware. I had problems before i did, and no longer have problems after resetting them.
Easy to Use By Victor on 3/11/2014Victor would recommend this product to a friendThe Director made setting up my Hydra's easy, it did have a few bugs with the wireless, maybe because it wasn't connecting to my wireless AP, I was able to solve that issue by wiring it up to my network.
A little disappointed By Mike on 3/2/2014Mike would recommend this product to a friendI bought this to control my Vegas figuring it would give me all the controllability that was promised with the Vegas. NOT....You have the same level of control as you do with Hydras. Bottom line...I could have saved $300 buying 3 Hydra fixtures and got purple and uv to boot. I spoke with AI about this and the promise more features will become available with future firmware upgrades but this has left a bit of a sour taste in my mouth. I didnt have any trouble connecting it to the internet or my lights as mentioned in other reviews. I guess overall I'm pleased with the Director it is much better than the previous controller option.
Love it but make sure you get the latest firmware By HM on 1/31/2014HM would recommend this product to a friendWhen I first got it I had problems we it from a faulty cable to requiring a manual update. But the AI support is really great. I alway got a response the same day.
I've had It up for about 2 months now and my corals are really responding great. I have my weather set to match the Barrier reef and it's cool to see the light change everyday. Stormy days are great when you see all the lightning.
Corals really like it too because since installing my flowerpot coral started giving me babies. Never did it before the new light fixture with weather matching and lunar cycle.
Works great after firmware updates By Frank on 1/26/2014Frank would recommend this product to a friendI ended up going with the AI lights rather than Eco Tech for my new reef. The deciding factor was the whole system would be cheaper. The Hydra 52 has 52 LEDs and 7 channels, and UV lights for $599 and the Radions only have 38 LED's, 6 channels, and no UV for $649. To top it off, the AI director is only $99 compared to $200 for Ecotechs Reef Link.
The director was rushed to market but I bought it later so the firmware updates had already fixed the problems people have experienced. It is basically a little black box that you control with your computer. I wire it directly to my network so I have now experience with issues you may encounter linking it to your wifi.
could be better By anthony on 1/21/2014anthony wouldn't recommend this product to a friendgot my director same time i got my hydra 52 (check for my review under hydra 52 if your considering it)
This product was nicely packaged, reminded me of the apple tv. the product was sleek and very clean, something you could put anywhere without creating an ugly area.
Now...... lets get to business.
It took me 3 days to completely set up the director. The firmware is not up to date with any WEP security or any updated systems for that matter. you would have to directly connect your director to the router via ethernet. This takes about 4-6 hours to update (on its own) and then you have to connect via wifi and wait another 1-2 hours for the software updates. AI was very helpful with this process so props to them on that. The little time i got to actually use the software of the director on my hydra it seemed very smooth but a little confusing if you have no working experience with computers and basic software manipulation. The stop/ start cycle game me some real issues as it would not start from the beginning of the day instead it would start at 4 instead of 1. This problem could of been easily adjusted but i did not have time or the patience as the humming of the hydra tempted me to throw it out the window. after i could no longer stand the sound emitting from my hydra i decided to return the whole setup after AI told me that this hummin/buzzin was normal and tested to a "tolerable" level. Good luck i hope they fix all the kinks
On the fence By BAJAB on 1/21/2014BAJAB wouldn't recommend this product to a friendIt was a little frustrating at first to get it configured, but will stabilize once you're able to update the firmware (which could take a day to show up as an option). The only bug that I'm dealing with now is the "manual" function sometimes does not working on my mobile device. I bought this for the new Hydra 52 which is an excellent light, but it is severely handicapped without this controller so I really didn't have a choice, which is a little upsetting and a slap in the face given the bugs that I had to deal with.
There are no videos related to this product.
Previously Asked Questions
by Meirav Taub on April 3, 2014, @7:53 AM
Do I also need the white controller or the director replaces it?
Bulk Reef Supply Hi Meirav,
You need either the director or the AI controller but you do not need both.
by Brian on November 22, 2013, @3:15 AM
Will this work with a sol blue fixture with the a wired controller ? Do I need to get anything else to make my light work with the director?
Bulk Reef Supply Hi Brian,
To use it with an AI Sol you would need to purchase the wireless adapter for the sol.
LED Controls
- Lunar Cycle
- Coral Acclimation
- Weather - cloud sweeps, lightning or pull live weather from user configured locations
- Play your entire day's timer configuration in fast forward as a Test Run
- PowerPuck Technology - dynamically recognizes changes in LED configurations
- Compatible with Mac, PC and iPhone
- Cloud-based service that allows you to connect all your AI devices and control them from anywhere
What's in the Box
- AI Director
- Power Supply
- USB Power Cable
- Wi-Fi Adapter
Deal of the Day!
Skimz Monzter SM203 DC Internal Protein SkimmerEnds in
Regular Price: $499.99
Special Price: $349.9900:00:00:00
Deal of the Day!
Skimz Monzter SH1 Hang On Internal SkimmerEnds in
Regular Price: $121.99
Special Price: $85.3900:00:00:00
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Main: 763-432-9691
Fax: 763-710-4777
Phone Hours: 7am - 7pm CST M-Th
10am-4pm CST Friday
Email: email@example.com
6325 Sandburg Road, Suite 400
Golden Valley, MN 55427 Map
View Larger Map 6325 Sandburg Road, Suite 400, Golden Valley, MN 55427


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Point: OH FUCK! OFFICER HELP! HE SHOT ME! Oh man…so much blood…oh it hurts like a motherfucker! Officer, this man tried to rob me at gunpoint! I threw all I had at him then turned to run away! He's insane, he shot me in the leg when I was trying to get out of there!
Counterpoint: You can't prove nuthin man, nuthin! Man I was just walkin down the street over there and man you come runnin round the corner next to that bookstore and the next thing I know you ran right into me and my self defense handgun goes off! Man then you crazy flailing around and bleeding took out your wallet and threw it at me! Man, you crazy man!


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I really don't know what to think about this one. The song's been like haunting me for weeks now. And with every word, I could clearly see a robsten footage....
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Royal Icing Recipe
Royal icing goes on smooth and dries with a hard finish, so it's ideal for decorating. Spread this royal icing recipe over your favorite sugar cookies. If you're making a royal icing recipe for children, pregnant women, or anyone with compromised immune systems, use meringue powder, found in the baking aisle of most markets, instead of raw egg whites.
- Yield: Makes 2 1/2 cups
Source: Martha Stewart Weddings, Winter 2002
- 1 pound confectioners' sugar
- 5 tablespoons meringue powder
- Scant 1/2 cup water
Beat ingredients in a mixer bowl on low speed until smooth, about 7 minutes. If icing is too thick, add more water; if too thin, beat 2 to 3 minutes more.
Cook's Note
Reviews (27)
shaoi 17 Mar, 2012
I think I just found my go-to royal icing recipe. It is just sweet enough and my family enjoyed it. I have about a cup left over after icing 28 medium-sized cookies for St. Patty's Day. I used it with the Ideal Sugar Cookie recipe. Thank you, Martha and staff!Reply
bibliobethica 26 Feb, 2011
I noticed Danni B is now selling her royal icing. Makes me wonder, what is the shelf life of the frosting? I always make too much.Reply
Cally6 12 Feb, 2011
And when using egg whites, do not add the water. I add a couple of tsps. of lemon juice for thinner consistency.Reply
Cally6 12 Feb, 2011
In response to dancer65, when using egg whites, place all ingredients in bowl together and mix.Reply
amotn 18 Dec, 2010
This is NOT an egg free frosting. This could cause a severe allergic reaction if you cannot eat eggs. Imagine some one telling a friend that the frosting is egg free when it is not. This needs to be renamed or removed.Reply
dancer65 16 Dec, 2010
If you are using the egg whites, do you beat the eggs whites first and then add the sugar or do you mix then together and beat themaat the same time? I am making the cookies now and need to know how to do the icing. ThanksReply
MaryGirlMomOf5 11 Dec, 2010
If using egg whites, please be aware of the risk of Salmonella. Raw egg products should never be given to babies, small children, the elderly, or anyone with a compromised immune system. Healthy adults who choose to consume raw eggs or raw eggs products should be made aware of the risk of food bourne illness.Reply
Cally6 19 Oct, 2010
Royal Icing Using Egg Whites: 2 large egg whites, 2 tsps. fresh lemon juice, and 3 cups confectioners (icing) sugar, sifted. Mix this altogether by hand and divide into small glass custard cups. I add a tiny bit of Wilton Icing Color gel to each cup for various gorgeous colors. My kids use water paintbrushes to ice the cookies!Reply
crazymomma2day 4 May, 2010
going to try the egg white instead of meringue powder. recipe doesnt say anything about adding color to the piping icing. Divide up once mixed and add food coloring for piping in color? any experiences, thoughts on this appreciated.Reply
kniymc 19 Mar, 2010
if you're using egg whites, you don't need water at all for piping consistenecy, but you will need a small amount for flooding. If you use meringue powder, then use the water.
notice that the directions here don't mention the egg whites... these directions are for the meringue powder only.Reply
keltaylor19 24 Dec, 2009
I would suggest not using the water at all unless needed. I only needed about 1 T to make the icing. My kids also loved helping me. quick and easyReply
KristinWilliams 22 Dec, 2009
I used this receipe today and discovered that even a scant 1/2 cup of water is toooo much if you use egg whites. I ended up cutting in another 1 1/4 cups confectioners' sugar to make it stiff enough to do outlines.Reply
macpoodle 24 Nov, 2009
how long can you keep this frosting?Reply
valeriethomas 10 Oct, 2009
it's not too late to slowly add up to 1/2 cup of water.Reply
allergyfreekids 2 Jul, 2009
This is not an egg-free recipe and should not be labeled as one. Meringue powder has egg whites in it and if it where served to anyone with an egg allergy they could have a very serious adverse reaction. If you tell someone it's egg free than it should truly be egg free or you could kill them.Reply
bacraig 2 Jul, 2009
Walmart stores carry Meringue powder in the craft section, in the Wilton products section. Larger Penny's stores carry Wilton as well.Reply
pukachelle 21 Jun, 2009
In the process of mixing and it's unclear.. too late NOW, but maybe not next time..
If you opt for egg whites instead of meringue powder do you still add the scant 1/2 cup of water???Reply
pukachelle 21 Jun, 2009
Well I am mixing it now.. and it is too late to get feedback to help.. grrr
Do you add the scant 1/2 cup of water if you opted for egg whites over meringue powder??Reply
kristinafrench 2 Dec, 2008
Has an egg-free royal icing. Hope that helps!Reply
Evans_mom 28 Nov, 2008
This is not really egg free, as meringue powder has dried egg whites in it. Is there any recipe that is really "egg free" for royal icing? My son has an egg allergy and I wanted to use royal icing to make him an egg free gingerbread house for the holidays.Reply
Evans_mom 28 Nov, 2008
This is not really egg free, as meringue powder has dried egg whites in it. Is there any recipe that is really "egg free" for royal icing? My son has an egg allergy and I wanted to use royal icing to make him an egg free gingerbread house for the holidays.Reply
lisa_0225 27 Oct, 2008
you can also purchase meringue powder at Walmart in the cake decorating section.Reply
jenny717 3 Jul, 2008
I got some at JoAnn Fabircs for about $5 for 4 oz.Reply
BakingBarrister 3 Jul, 2008
It is also sold at Williams SonomaReply
reunderly 2 Jul, 2008
You can get meringue powder at Michael's crafts, Total Crafts and AC Moore. Look for it at those stores too.Reply
cleongatl 2 Jul, 2008
Meringue powder is currently very difficult to find. Plan ahead and order it online. Otherwise, search for cake decorating supply stores in your area to find a retial outlet.Reply
zionrubies 21 Mar, 2008
I didn't know about merigue powder before seeing this recipe. My iced Valentine's Day cookies turned out beautiful and for once made it through the mail looking as good as they did when I sent them.Reply


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It is possible for credit cards to assist people throughout the world in attaining their lifestyle goals. Having one gives an individual increased financial freedom. Keeping this in mind, it's crucial that you carefully pick your credit card and use it wisely. This article is a great place to start learning about credit card options and how to make educated credit decisions.
Whenever you spot anything fraudulent on your monthly credit card statement, call the issuing company right away. This gives the credit card companies the most opportunity to catch the person responsible. That is also the smartest way to ensure that you aren't responsible for those charges. A quick call or simple email may be all that is required for you to report a fraudulent charge.
A lot of cards have sign-up bonuses. Make certain you completely understand fine print in the terms and conditions though, because a lot of the credit card companies have very particular terms for you to qualify for before you get the bonus. Many times, the creditor will require you to charge a particular amount in a set time to receive the bonus.
Make a realistic budget to hold yourself to. You do not need to spend the entire limit on your card, even though it's available. Be sure of how much you are able to pay every month so you're able to pay everything off monthly. This will help you stay away from high interest payments.
Before signing up for any credit card, make sure that you comprehend the terms and conditions fully. It is possible to discover rates that are higher than you expected. Pay very close attention to the terms to make sure you completely understand the card policy.
Set up a budget you can remain with. Keep in mind that your credit card company is not providing you with a target to aim for when they set a credit limit on your account. It is important to stay within your budget and do not charge more than you can pay when the bill comes due.
Credit cards can offer many advantages and increase spending options to create a better lifestyle for consumers. They can be an amazing tool for opening financial doors, or they can be the reason for financial doors being slammed closed.
As was mentioned earlier, it is very easy to find yourself in financial trouble when you improperly use credit cards. If you have just a couple more cards than you need or buy just a few more things than you can afford to pay for, you'll quickly get into trouble. Hopefully, this article will help you to avoid credit card problems and keep yourself financially healthy.
Learn about dealing with failure . While you're here, pick up our FREE 30 Day Meal Plan and start slimming down now.


text:
I went to surya sangolda over new year. The apartment I was staying in was amazing, furnished to a really high standard and was really clean. The staff go above and beyond what is expected to make your stay really comfortable. They knocked on my door after I arrived with breakfast, whenever I needed to go somewhere they offered to... More
- Reservation Options:
- TripAdvisor is proud to partner with Agoda, Booking.com, Expedia, Hotels.com, Travelocity, TripOnline SA, Odigeo and Tingo so you can book your Surya Sangolda reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.


text:
Sign up to be notified about Lawson Ice Arena and Gabel Natatorium event changes, reminders and cancellations by email. Fill out the form and check those events you would like to be notified of when they are cancelled.
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WMU Bronco Hockey
Lawson Ice Arena and Gabel Natatorium
Western Michigan University
Kalamazoo MI 49008-5335 USA
(269) 387-3050 | (269) 387-3051 Fax | Site firstname.lastname@example.com


text:
Tweezing is way too painful to be glamorous or pampering. But if you add a little Betsey Johnson to the experience, you can at least feel fabulous while you pluck. Betsey has teamed up with Tweezerman to create the Betsey Johnson Slant Tweezer Collection. Choose from three options: black with a "Marilyn" face, neon pink with white skulls, or white with pink rosebuds. Today is the day to start plucking in style.
[$25.00 Sephora]


text:
Channel 6 News - Search Results
Pittsfield Police seize drugs, cash
PITTSFIELD -- Pittsfield Police seized large amounts of drugs and cash on Wednesday.
The department's drug unit, along with the Berkshire County response team and the county drug task force say they seized 42 individual baggies of crack cocaine after executing a search warrant at 219 Wahconah Street. Police say the bags were packaged for sale and had a street value of more than $2,000. They also seized more than $2,300 in cash from the home.
Authorities have not announced any arrests related to the seizure, but say more information will be released.


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Design a new 59 Belts buckle
59 Belts has partnered with PGA Tour player Jamie Lovemark to offer fans an opportunity to create a new "JL" belt buckle for the 2010 season.
Commentary: Recari sparkles as Mojo 6 kicks off
There are 12 LPGA players in Jamaica this week for the Mojo 6 event, but Beatriz Recari is the only one who was voted in by her fans. (And now it's time to play Raceway Golf!) Beth Ann Baldry explains.
Zinger, you have something on your head
Ready for a good laugh? When Paul Azinger met with the media prior to the Champions Tour's Outback Steakhouse Pro-Am, one writer wondered if falling debris had ever derailed a putt of his the way it knocked Phil Mickelson's 12-foot downhill birdie putt off line on the second hole Masters Sunday.
Charleston Southern wins Big South title
Charleston Southern won its sixth Big South Women's Championship title Wednesday.
Stetson rallies to win A-Sun Championship
Stetson used a record-breaking performance Wednesday to win the Atlantic Sun Conference Championship.
Garza determined to earn NCAA trip
Dustin Garza may say he isn't disappointed that he was left off the Ben Hogan Award's semifinalist list, but I'm not buying it. This award looks great on anyone's resume and any golfer would be thrilled to win it.
Hogan Award semifinalists named
The 10 semifinalists for the 2010 The Ben Hogan Award were annonuced Wednesday.
Thumbs up: Yale | Thumbs down: Garza snub
It's Wednesday which means it's time for thumbs up and thumbs down.
Cink soaks up Augusta's new range
Players cannot wait to get to the Masters. Now we can tell you that there are some who refuse to leave – even if they've missed the cut.
Talking fashion with Webb Simpson
I spoke recently with PGA Tour player Webb Simpson. He has posted two top-20 finishes this season on Tour and told me about his fashion expertise.
Commentary: Hate to be Rude: Lefty's gamble = jackpot
Phil Mickelson's shot to the 13th green on Masters Sunday was the fitting moment that will define his Hall of Fame career. Jeff Rude explains.


text:
summarizes the two presentations that focused on methodologies and limitations of attaching monetary value to costs and benefits.
Chapters 6 and 7 summarize group discussion that occurred throughout the course of the workshop, including discussion that occurred during the small working group portion of the workshop. About one-third of the workshop time was spent in small working groups. There were four working groups: energy usage and GHG emissions; soil, water, and other environmental consequences; consequences of antimicrobial use in agriculture; and other public health consequences. The groups were asked to identify effects, methodologies for measuring those effects, and limitations of the methodologies. Chapter 6 includes a summary of these working group discussions. Chapter 7 provides an overview of the major overarching themes from all the open discussions that occurred throughout the workshop, including participants' reflections on key considerations for moving forward with future work in this area.
This workshop summary was prepared by the rapporteurs as a factual summary of the presentations and discussions that took place during the
Key Terms Used in This Report
End-of-life: In the context of LCA, end-of-life refers to the stage of the product after preparation and consumption by the conumer. At this stage, the food product is disposed of in some manner (e.g., recycled or placed in a landfill).
Health impact assessment (HIA): HIA is not a single method, but rather a systematic process that uses a wide array of data sources, analytical methods, and stakeholder input to determine the potential effects of a proposed policy, plan, program, or project on the health of a population and the distribution of those effects within the population.
Life cycle assessment (LCA): In the context of the food system, LCA is a tool for examining the environmental impact of a product that covers the impacts of manufacturing, of the upstream production chain (e.g., material extraction, fuels, transportation, etc.) and downstream disposal (e.g., recycling, landfilling, etc.). According to Heller and Keoleian (2003), "a product life cycle approach provides a useful framework for studying the links between societal needs, the natural and economic processes involved in meeting these needs, and the associated environmental consequences."
Life cycle stages: For a food product, the following life cycle stages are considered in the context of economic, social, and environmental sustainability indicators: the origin of the product; agricultural and production conditions; processing, packaging, and distribution of the product; preparation and consumption by the consumer; and the end-of-life of the product (Heller and Keoleian, 2003).


text:
Research Topics
Genomes and Genes
adenovirus e1 proteins
Summary: The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
Top Publications
Research Grants
Scientific Experts
Detail Information
- An adenovirus type 5 (Ad5) amplicon-based packaging cell line for production of high-capacity helper-independent deltaE1-E2-E3-E4 Ad5 vectorsDaniele Catalucci
Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 600, 00040 Pomezia, Roma, Italy
J Virol 79:6400-9. 2005..6 kb) was exploited to construct a vector encoding the entire hepatitis C virus (HCV) polyprotein. Infection of HeLa cells by the resulting vector showed high levels of correctly processed HCV proteins...
- Efficient generation and amplification of high-capacity adeno-associated virus/adenovirus hybrid vectorsManuel A F V Gonçalves
Gene Therapy Section, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
J Virol 76:10734-44. 2002..98% without compromising hybrid vector rescue. This allowed amplification of high-capacity AAV/Ad hybrid vectors to high titers in a single round of propagation...
- Infection of endothelium with E1(-)E4(+), but not E1(-)E4(-), adenovirus gene transfer vectors enhances leukocyte adhesion and migration by modulation of ICAM-1, VCAM-1, CD34, and chemokine expressionS Rafii
Division of Hematology Oncology, Belfer Gene Therapy Core Facility, Institute of Genetic Medicine, Cornell University Medical College, New York, NY, USA
Circ Res 88:903-10. 2001..inflammatory responses by upregulating expression of adhesion molecules and enhancing migration through Advector-infected ECs and suggest that E1(-)E4(-) Advectors may be a better choice for gene-transfer strategies directed to the ECS:..
- Construction of adenovirus vectors through Cre-lox recombinationS Hardy
Somatix Therapy Corporation, Alameda, California 94501 1034, USA
J Virol 71:1842-9. 1997..We found that a loxP site next to the packaging site of the gutless virus was necessary to neutralize homologous recombination between psi5 and the gutless viruses within their packaging domains...
- Human adenovirus type 35: nucleotide sequence and vector developmentW Gao
Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA
Gene Ther 10:1941-9. 2003..Our novel Ad35-based vectors and their corresponding packaging cell lines will provide a versatile and powerful system for DNA-based vaccine development and gene therapy applications...
- Adenoviral-mediated transduction of human pancreatic islets: importance of adenoviral genome for cell viability and association with a deficient antiviral responseAndreea R Barbu
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
Endocrinology 146:2406-14. 2005....
- Development and characterization of bovine x human hybrid cell lines that efficiently support the replication of both wild-type bovine and human adenoviruses and those with E1 deletedAlberto L van Olphen
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, USA
J Virol 76:5882-92. 2002..The development of somatic cell hybrids appears to be a simple way of combining some of the desirable characteristics present separately in two parental cell lines...
- Incorporating the survivin promoter in an infectivity enhanced CRAd-analysis of oncolysis and anti-tumor effects in vitro and in vivoZeng B Zhu
Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, 35291, USA
Int J Oncol 27:237-46. 2005..These data clearly demonstrate that CRAd-Survivin-RGD is a potential novel therapeutic agent for treatment in many, but not all, human cancers...
- Towards a double controlled conditionally replicative adenovirus for potent and specific melanoma cell killW M Gommans
Department of Therapeutic Gene Modulation, University of Groningen A Deusinglaan 1, Groningen, The Netherlands
J Control Release 116:e64-6. 2006
- Gene expression changes induced by a recombinant E1-/E3- adenovirus type 5 vector in human mammary epithelial cellsAngelo G Scibetta
Cancer Research UK, Breast Cancer Biology Group, Guys Hospital, London, UK
Intervirology 48:350-61. 2005..Gene expression changes induced by an E1/E3-defective adenovirus vector have been studied in human mammary epithelial cells by comparing the gene expression profile in infected and uninfected cells...
- Binary AdEasy vector systems designed for Tet-ON or Tet-OFF regulated control of transgene expressionSaideh Berenjian
Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 751 23 Uppsala, Sweden
Virus Res 115:16-23. 2006..Taken together our results suggest that the binary vectors described here should be a valuable addition to the repertoire of viral vectors used in basic and medical research...
- Induction of CD8+ T cells to an HIV-1 antigen through a prime boost regimen with heterologous E1-deleted adenoviral vaccine carriersArguinaldo R Pinto
The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
J Immunol 171:6774-9. 2003..They are equivalent to sequential vaccinations with an E1-deleted Ad vector followed by booster immunization with a poxvirus vector and they surpass regimens based on DNA vaccine prime followed by a recombinant adenoviral vector boost...
- Combination therapy with conditionally replicating adenovirus and replication defective adenovirusChoon Taek Lee
Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37322 6838, USA
Cancer Res 64:6660-5. 2004..The combination of CRAD and E1-deleted ad induced tumor-specific replication of CRAD and E1-deleted ad and increased the transduction rate and therapeutic efficacy of these viruses in model tumors...
- Improved vascular gene transfer with a helper-dependent adenoviral vectorShan Wen
Department of Medicine, University of Washington, Seattle 98195 7710, USA
Circulation 110:1484-91. 2004....
- Fluorescently labeled adenovirus with pIX-EGFP for vector detectionLong P Le
University of Alabama, Birmingham, USA
Mol Imaging 3:105-16. 2004..In addition, this technique has potential utility for dynamic monitoring of adenovirus replication and spread...
- Prolongation of islet allograft survival following ex vivo transduction with adenovirus encoding a soluble type 1 TNF receptor-Ig fusion decoyJ Machen
Diabetes Institute, Children s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Gene Ther 11:1506-14. 2004..These data support the potential utility of TNFR-Ig gene transfer to islets as a means of facilitating allogeneic islet transplantation...
- A simian replication-defective adenoviral recombinant vaccine to HIV-1 gagJulie C Fitzgerald
The Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 170:1416-22. 2003..However, the response elicited by the AdHu5 vaccine is greatly attenuated in AdHu5 preimmune animals...
- Hybrid yeast-bacteria cloning system used to capture and modify adenoviral and nonviral genomesCraig A Hokanson
Cell Genesys, 342 Lakeside Drive, Foster City, CA 94404, USA
Hum Gene Ther 14:329-39. 2003..This novel cloning strategy allows the rapid and versatile construction of adenoviral vectors for gene expression and gene therapy applications...
- An HSV amplicon-based helper system for helper-dependent adenoviral vectorsShuji Kubo
Department of Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA, USA
Biochem Biophys Res Commun 307:826-30. 2003..Since the HSV amplicon can be easily and completely inactivated by chloroform extraction and/or heat treatment from the HDAdV stock, this helper system might be an alternative method to produce helper virus-free HDAdVs...
- A recombinant E1-deleted porcine adenovirus-3 as an expression vectorAlexander Zakhartchouk
Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada
Virology 313:377-86. 2003..We demonstrated that PAV219 was able to transduce and direct expression of GFP in some human cell lines...
- Early adenoviral gene expression mediates immunosuppression by transduced dendritic cell (DC): implications for immunotherapy using genetically modified DCAndrea Tuettenberg
Department of Dermatology, J Gutenberg University, Mainz, Germany
J Immunol 172:1524-30. 2004..These results have important implications for the use of genetically modified DC for therapeutic application...
- An adenoviral system for tetracycline-regulated TGF-beta expression mediates a reversible cell cycle arrestM A Wettergreen
Department of Oral Medicine and Diagnostic Sciences, College of Dentistry, University of Illinois at Chicago, USA
Eur J Oral Sci 109:415-21. 2001..These results highlight the possibility of using low level infection with recombinant adenovirus to cause short-term blocks on cell proliferation...
- Configurations of a two-tiered amplified gene expression system in adenoviral vectors designed to improve the specificity of in vivo prostate cancer imagingM Sato
Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1738, USA
Gene Ther 15:583-93. 2008..The prostate-targeted gene delivery vectors with robust and cell-specific expression capability will advance the development of safe and effective imaging guided therapy for recurrent metastatic stages of prostate cancer...
- Direct comparison of hepatocyte-specific expression cassettes following adenoviral and nonviral hydrodynamic gene transferF Jacobs
Molecular and Cardiovascular Medicine, Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
Gene Ther 15:594-603. 2008....
- Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditionsAngélica Meneses-Acosta
Animal Cell Technology Group, Biotechnology Research Institute, National Research Council, 6100 Royalmount Avenue, Montreal, Quebec, Canada H4P 2R2
J Virol Methods 148:106-14. 2008..These results were validated in a 3 L bioreactor under controlled operating conditions where a higher HDAd yield of 2.6 x 10(9) viral particles (VP)/mL or 3.5 x 10(8) infectious units (IU)/mL of HDAd was obtained...
- T-cell response to adenovirus hexon and DNA-binding protein in miceT McKelvey
Department of Viral Vaccine Research, Merck Research Laboratories, West Point, PA, USA
Gene Ther 11:791-6. 2004..These results add to the evidence that cell-mediated immunity is a substantial barrier to therapeutic adenoviral vectors and provide more quantitative tools to measure cellular immune responses to adenovirus...
- A replication-competent adenovirus assay for E1-deleted Ad35 vectors produced in PER.C6 cellsG Marzio
Crucell Holland BV, P O Box 2048, 2301CA Leiden, The Netherlands
Vaccine 25:2228-37. 2007..C6 cells were analysed and found to be free of RCA and HDEP. Based on the statistical model used, we anticipate that our approach to RCA assay development can be broadly applicable to other adenoviral vectors...
- Rapid construction of small interfering RNA-expressing adenoviral vectors on the basis of direct cloning of short hairpin RNA-coding DNAsHiroyuki Mizuguchi
National Institute of Biomedical Innovation, Osaka 567 0085, Japan
Hum Gene Ther 18:74-80. 2007..This method should be useful for RNA interference-based experiments, and should make it easy to construct an siRNA-expressing Ad vector library for functional screening...
- Intravenous administration of an E1/E3-deleted adenoviral vector induces tolerance to factor IX in C57BL/6 miceP A Fields
Departments of Pediatrics and Pathology, University of Pennsylvania Medical Center and The Children's Hospital of Philadelphia, Philadelphia, PA, USA
Gene Ther 8:354-61. 2001..Given the well-documented immunogenicity of the first-generation adenoviral vector, data from C57BL/6 mice may therefore grossly underestimate immunological consequences in certain gene therapy protocols...
- Improved adenoviral vector for vascular gene therapy : beneficial effects on vascular function and inflammationH S Qian
Duke University Medical Center, Durham, NC, USA
Circ Res 88:911-7. 2001....
- A novel system to study adenovirus tropism to normal and malignant colon tissuesD Kolodkin-Gal
Department of Virology, The Hebrew University Hadassah Medical School, The Hebrew University, Jerusalem, 91120, Israel
Virology 357:91-101. 2007..Increased infectivity and altered tropism were noted in the treated tumor tissue. Taken together, this ex vivo system indicated that receptor utilization and extracellular-matrix components influence AD viral tropism in solid tissues...
- Fiber-substituted conditionally replicating adenovirus for oncolysis of human renal carcinoma cellsHisao Nagaya
Laboratory of Cell and Gene Therapy, Institute for Advanced Medical Sciences, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya 663 8501, Japan
Anticancer Res 32:2985-9. 2012..We investigated the oncolytic effects of a chimeric adenovirus type 5 (Ad5) with replacement of Ad5 fiber knob with adenovirus type 35 (Ad35) fiber knob (Ad5F35) on human renal cell carcinoma (RCC)...
- Cell transformation by human adenovirusesC Endter
Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg, Landshuterstr 22, 93047 Regensburg, Germany
Curr Top Microbiol Immunol 273:163-214. 2004....
- Liver toxicities typically induced by first-generation adenoviral vectors can be reduced by use of E1, E2b-deleted adenoviral vectorsR S Everett
Department of Pediatrics, Division of Medical Genetics, Duke University Medical Center, Durham, NC 27705, USA
Hum Gene Ther 14:1715-26. 2003..On the basis of a careful review of the literature, these improvements in vector safety rival those noted with other, more significantly modified Ad vectors described to date...
- Characterization of human adenovirus 35 and derivation of complex vectorsDuncan McVey
Department of Research, GenVec, Inc, Gaithersburg, MD 20874, USA
Virol J 7:276. 2010..The structure of the viral genome and life cycle of Ad35 differs from the better characterized Ad5 and these differences require differences in the strategies for the generation of vectors for gene delivery...
- Common structure of rare replication-deficient E1-positive particles in adenoviral vector batchesPete Murakami
Process Development Department, Berlex Biosciences, 2600 Hilltop Drive, Richmond, CA 94804, USA
J Virol 78:6200-8. 2004..This finding allowed development of an HDEP assay to screen batches of rAd5 produced on the PER.C6 cell line and resulted in detection of seven HDEP agents from four different transgene-virus vector constructs in separate batches of Ad...
- Characterization of a family of chimpanzee adenoviruses and development of molecular clones for gene transfer vectorsSoumitra Roy
Department of Medicine, University of Pennsylvania and The Wistar Institute, Philadelphia, PA 19104, USA
Hum Gene Ther 15:519-30. 2004..These results indicate that chimpanzee adenoviral vectors may be useful as vaccines or gene therapy vectors in human populations and should allow applications that require multiple vector administrations...
- NKG2D is required for NK cell activation and function in response to E1-deleted adenovirusJiangao Zhu
Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
J Immunol 185:7480-6. 2010....
- Optimization of adenovirus serotype 35 vectors for efficient transduction in human hematopoietic progenitors: comparison of promoter activitiesF Sakurai
Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Osaka, Japan
Gene Ther 12:1424-33. 2005..These results indicate the construction of an optimized Ad35 vector backbone for efficient transduction into HSCs/progenitors...
- Checkpoints in adenoviral production: cross-contamination and E1ADagmar J Haeussler
Vascular Biology Section, Boston University Medical Center, Boston, Massachusetts, United States of America
PLoS ONE 6:e23160. 2011..Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA...
- Telomerase-specific replication-selective virotherapy for human cancerTakeshi Kawashima
Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
Clin Cancer Res 10:285-92. 2004..We investigated the antitumor effect of the hTERT-specific replication-competent adenovirus on human cancer cells...
- CpG methylation of a plasmid vector results in extended transgene product expression by circumventing induction of immune responsesA Reyes-Sandoval
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
Mol Ther 9:249-61. 2004..Nevertheless, mice remain responsive to the transgene product delivered by a viral vector...
- Optimization of vaccine responses with an E1, E2b and E3-deleted Ad5 vector circumvents pre-existing anti-vector immunityT Osada
Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
Cancer Gene Ther 16:673-82. 2009..These pre-clinical studies with E1 and E2b-deleted recombinant Ad5 vectors suggest that anti-Ad immunity will no longer be a limiting factor, and that clinical trials to evaluate their performance are warranted...
- Designing E1 deleted adenoviral vector by homologous recombinationAlireza Zamani
Dept of Immunology and Hematology, Medical School, Hamedan University of Medical Sciences, Hamedan, Iran
Iran Biomed J 11:199-202. 2007..In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generation of large collection of clones...
- Specific and efficient transduction of Cochlear inner hair cells with recombinant adeno-associated virus type 3 vectorYuhe Liu
Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minami Kawachi, Kawachi, Tochigi, Japan
Mol Ther 12:725-33. 2005..These findings are of value for further molecular studies of cochlear inner hair cells and for gene replacement strategies to correct recessive genetic hearing loss due to monogenic mutations in these cells...
- Rescue of chimeric adenoviral vectors to expand the serotype repertoireSoumitra Roy
Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, Gene Therapy Program, Translational Research Laboratory, 125 South 31st Street, Suite 2000, Philadelphia, PA 19104 3403, USA
J Virol Methods 141:14-21. 2007..It is further shown that this strategy may be more widely applicable...
- Immunological insights from genetic vaccinesHildegund C J Ertl
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
Virus Res 111:89-92. 2005..Virus neutralizing antibody titers can be enhanced by prime-boost regimens...
- AdEasy-based cloning system to generate tropism expanded replicating adenoviruses expressing transgenes late in the viral life cycleM Lie-A-Ling
AMC Liver Center, Academic Medical Center University of Amsterdam, The Netherlands
Gene Ther 12:1347-52. 2005..This flexible cloning system allows for straightforward construction of tropism expanded replicating adenoviral vectors that express transgenes late in the adenoviral life cycle...
- Replication-defective adenovirus serotype 5 vectors elicit durable cellular and humoral immune responses in nonhuman primatesSampa Santra
Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, RE113, P O Box 15732, Boston, MA 02215, USA
J Virol 79:6516-22. 2005..Therefore, administration of ADV5-based vectors in DNA-primed subjects may be a preferred use of this vaccine modality for generating long-term immune protection...
- Efficient generation of double heterologous promoter controlled oncolytic adenovirus vectors by a single homologous recombination step in Escherichia coliDennis Hoffmann
Ruhr University Bochum, Institute of Microbiology and Hygiene, Department of Molecular and Medical Virology, Bldg, MA, RM, 6 40, D 44801 Bochum, Germany
BMC Biotechnol 6:36. 2006..Presently the E1 and E4 regions are modified in a time consuming sequential fashion...
- Targeting mesothelioma using an infectivity enhanced survivin-conditionally replicative adenovirusesZeng B Zhu
Department of Medicine, Pathology, and Surgery, University of Alabama at Birmingham, Birmingham, Alabama 35291, USA
J Thorac Oncol 1:701-11. 2006..These agents should provide important insights into the identification of novel therapeutic strategies for mesothelioma...
- The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosisY Imai
Institute for Virus Research, Kyoto University, Japan
Nature 398:777-85. 1999..Overexpression of full-length FLASH blocks the anti-apoptotic effect of the adenovirus protein E1B19K. FLASH is therefore necessary for the activation of caspase-8 in Fas-mediated apoptosis...
- Influence of E1-deleted recombinant adenoviruses on B7.1 and IL-2 expression in C1498 cellsMohammad Saeid Hejazi
Dept of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Iran Biomed J 11:153-60. 2007..Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Delta) in induction of B7.1 and IL-2 molecules was studied...
- Decreased replication ability of E1-deleted adenoviruses correlates with increased brain tumor malignancySubrata Ghosh
Department of Neurological Surgery, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
Cancer Res 65:8936-43. 2005..Because of this tumor grade-based differential growth, the E1-deleted adenoviruses may represent novel tools for studies of brain tumor malignancy...
- [Inheritable phenotypic normalization of rodent cells transformed by simian adenovirus SA7 E1 oncogenes by singled-stranded oligonucleotides complementary to a long region of integrated oncogenes]N I Grineva
Hematology Research Center, Russian Academy of Medical Sciences, Moscow 125167 Russia
Genetika 31:1043-51. 1995..The normalization also produced cells that divided 2 - 5 times and died and cells that reverted to a transformed phenotype in 2 - 10 days. The latter appeared predominantly upon the action of the antisense oligonucleotides...
- Induction of endogenous genes following infection of human endothelial cells with an E1(-) E4(+) adenovirus gene transfer vectorR Ramalingam
Division of Pulmonary Medicine, Weill Medical College of Cornell University New York Presbyterian Hospital, New York, New York 10021, USA
J Virol 73:10183-90. 1999....
- Gene therapy in the inner ear. Mechanisms and clinical implicationsT R Van De Water
Department of Otolaryngology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Ann N Y Acad Sci 884:345-60. 1999..Gene therapy has been successfully applied to the cochlea of a laboratory animal, and future studies will define the types of vectors and therapeutic genes that will work best for the treatment of inner ear diseases in the clinic...
- Differential influence of the E4 adenoviral genes on viral and cellular promotersL Grave
Transgene SA, Strasbourg, France
J Gene Med 2:433-43. 2000..In this study, the influence of the E4 adenoviral region on the activity of various promoters was investigated in vitro and in vivo...
- An immunomodulatory procedure that stabilizes transgene expression and permits readministration of E1-deleted adenovirus vectorsA I Kuzmin
Institute for Gene Therapy and Molecular Medicine, Mt. Sinai School of Medicine, New York, New York 10029, USA
Mol Ther 3:293-301. 2001..These experiments demonstrate that this treatment is transient and antigen-specific...
- Biology of E1-deleted adenovirus vectors in nonhuman primate muscleP W Zoltick
Institute for Human Gene Therapy, University of Pennsylvania, USA
J Virol 75:5222-9. 2001....
- Development of a packaging cell line for propagation of replication-deficient adenovirus vectorJ S Kim
Clinical Research Center, Samsung Biomedical Research Institute, Seoul, Korea
Exp Mol Med 33:145-9. 2001..These results may have a significant impact on the development of packaging cell lines for replication-deficient adenovirus production...
- A midkine promoter-based conditionally replicative adenovirus for treatment of pediatric solid tumors and bone marrow tumor purgingY Adachi
The Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Cancer Res 61:7882-8. 2001..We concluded that application of this replication-competent Ad to hematopoietic grafts could be a simple but effective procedure to achieve complete tumor cell purging...
- Rejection of intraocular tumors by CD4(+) T cells without induction of phthisisL R Schurmans
Department of Ophthalmology, Leiden University Medical Center, Leiden, The Netherlands
J Immunol 167:5832-7. 2001..Taken together, these observations demonstrate that CD4(+) T cells are able to eradicate MHC class II-negative tumors in an immune-privileged site without affecting surrounding tissues or the induction of phthisis...
- Reduced toxicity, attenuated immunogenicity and efficient mediation of human p53 gene expression in vivo by an adenovirus vector with deleted E1-E3 and inactivated E4 by GAL4-TATA promoter replacementL Ji
Department of Thoracic and Cardiovascular Surgery, University of Texas MD Anderson Cancer Center, Houston 77030, USA
Gene Ther 6:393-402. 1999....
- Switching transgene expression in the brain using an adenoviral tetracycline-regulatable systemT C Harding
Department of Medicine, University of Bristol
Nat Biotechnol 16:553-5. 1998..This regulatable adenoviral vector system should be of value in behavioral studies and in vivo studies of neuronal gene function, and may further the development of effective gene therapy strategies in the brain...
- Lack of persistence of E1- recombinant adenoviral vectors containing a temperature-sensitive E2A mutation in immunocompetent mice and hemophilia B dogsB Fang
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA
Gene Ther 3:217-22. 1996..Additionally, humoral immune response to transgene products has been demonstrated in immunocompetent animal models, which will also need to be surmounted for long-term efficacy in disease treatment by gene therapy...
- Unique genome arrangement of an ovine adenovirus: identification of new proteins and proteinase cleavage sitesS Vrati
Division of Biomolecular Engineering, CSIRO, North Ryde, New South Wales, Australia
Virology 220:186-99. 1996..The data emphasize that this ovine virus differs significantly from other known human and animal adenoviruses...
- Recombinational construction in Escherichia coli of infectious adenoviral genomesJ Crouzet
Rhône Poulenc Rorer Gencell, Centre de Recherche de Vitry Alfortville, Vitry sur Seine, France
Proc Natl Acad Sci U S A 94:1414-9. 1997..The potential of this procedure is demonstrated by the recovery of several E1E3E4-deleted adenoviruses following transfection of the corresponding E. coli-derived genomes in IGRP2 cells...
- The human cytomegalovirus major immediate early promoter can be trans-activated by adenovirus early proteinsC M Gorman
Department of Cell Genetics, Genentech, Inc, South San Francisco, California 94080
Virology 171:377-85. 1989We have examined the effect of adenovirus E1 proteins on expression from the immediate early (IE) region of the human cytomegalovirus (HCMV)...
- Conditional repression of the E2 transcription unit in E1-E3-deleted adenovirus vectors is correlated with a strong reduction in viral DNA replication and late gene expression in vitroK Rittner
Gene Therapy Department, Transgene S A, Strasbourg, France
J Virol 71:3307-11. 1997..In contrast, there was no repression in the presence of the regulator tetracycline. We propose that regulation by TetR-KRAB is a valuable tool with which to study the effects of viral gene expression in vitro...
- Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deletedA Amalfitano
Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA
J Virol 72:926-33. 1998..The results suggested that these modified vectors may be very useful both for in vitro and in vivo gene therapy applications...
- In vitro and in vivo biology of recombinant adenovirus vectors with E1, E1/E2A, or E1/E4 deletedM Lusky
Transgene S A, Strasbourg, France
J Virol 72:2022-32. 1998..In addition, our data confirm that, in the absence of transgene expression, mouse cellular immunity to viral antigens plays a minor role in the progressive elimination of the virus genome...
- Selective gene transfer into the liver of non-human primates with E1-deleted, E2A-defective, or E1-E4 deleted recombinant adenovirusesS E Raper
Institute for Human Gene Therapy, Harrison Department of Surgical Research, University of Pennsylvania School of Medicine, Philadelphia 19104 6100, USA
Hum Gene Ther 9:671-9. 1998..At high doses of vector, widespread dissemination of vector DNA is seen. At low doses, hepatic gene transfer is not associated with vector DNA dissemination to gonads...
- Effects of first generation E1E3-deleted and second generation E1E3E4-deleted/modified adenovirus vectors on human endothelial cell deathL Jornot
Respiratory Division, Geneva University Hospitals, Switzerland
Endothelium 8:167-79. 2001..This effect can be complicated by the action of transgene expression on cell survival...
- Single oral immunization with replication deficient recombinant adenovirus elicits long-lived transgene-specific cellular and humoral immune responsesSally Sharpe
Centre for Applied Microbiology and Research, Salisbury SP4 0JG, United Kingdom
Virology 293:210-6. 2002....
- Inclusion of Moloney murine leukemia virus elements upstream of the transgene cassette in an E1-deleted adenovirus leads to an unusual genomic integration in epithelial cellsChangyu Zheng
Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 1190, USA
Virology 313:460-72. 2003..These results suggest that the 2.7 kb of MoMLV sequence included in AdELP-luc have cis-acting functions and mediates an unusual integration event...
- Infectivity enhanced, cyclooxygenase-2 promoter-based conditionally replicative adenovirus for pancreatic cancerMasato Yamamoto
Division of Human Gene Therapy, University of Alabama at Birmingham, BMR2 408, 901 19th Street South, Birmingham, Alabama 35294 2172, USA
Gastroenterology 125:1203-18. 2003..Thus, we constructed infectivity enhanced cyclooxygenase (COX)-2 promoter-based CRAds to develop a safe and effective therapeutic modality...
- Mucosally delivered E1-deleted adenoviral vaccine carriers induce transgene product-specific antibody responses in neonatal miceZhiquan Xiang
Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 171:4287-93. 2003..The efficacy of the AdHu5rab.gp vector given orally to newborn mice born to AdHu5 virus-immune dams was not impaired by maternally transferred Abs to the vaccine carrier...
- Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transferSteven E Raper
Department of Surgery, University of Pennsylvania School of Medicine, BRB II III Rm, 607 421 Curie Blvd, Philadelphia, PA 19104, USA
Mol Genet Metab 80:148-58. 2003....
- Dawdling polymerases allow introns time to spliceNick J Proudfoot
Nat Struct Biol 10:876-8. 2003
- Replication of an adenoviral vector controlled by the human telomerase reverse transcriptase promoter causes tumor-selective tumor lysisNicholas A Lanson
Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, LA 70112, USA
Cancer Res 63:7936-41. 2003..These results suggest that the Ad5-hTERT-E1 vector has potential as a broad-spectrum antitumor agent...
- Adenovirus vector library: an approach to the discovery of gene and protein functionDuncan McVey
GenVec Inc, 65 West Watkins Mill Road, Gaithersburg, MD 20878, USA
J Gen Virol 84:3417-22. 2003..This AdLibrary approach provides an opportunity to exploit the efficient gene delivery capabilities of adenovirus vectors for the rapid discovery of gene and protein function...
- Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following subretinal injection in the ratWei Yong Shen
Centre for Ophthalmology and Visual Science, The University of Western Australia, 2 Verdun Street, Nedlands, Perth, WA 6009, Australia
Vision Res 44:339-48. 2004..Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases...
- Intraneural colchicine inhibition of adenoviral and adeno-associated viral vector remote spinal cord gene deliveryNicholas M Boulis
Department of Neurosurgery, The Cleveland Clinic Foundation, Lerner Research Institute, Cleveland, Ohio 44195, USA
Neurosurgery 52:381-7; discussion 387. 2003..The present experiment demonstrates that intraneural injection of colchicine is capable of inhibiting remote delivery of both adenoviral and adeno-associated viral (AAV) vectors, implicating axonal transport in this process...
- [A new human cellular protein AUP1. III. The intracellular localization of AUP1 protein in different human and rat cell lines]K V Karpisheva
Institute of Cytology RAS, St Petersburg, Russia
Tsitologiia 44:846-51. 2002..Taken together, these results suggest that AUP1 is a cytoplasmic protein that is expressed in all cell lines we examined...
- Efficient mobilization of E1-deleted adenovirus type 5 vectors by wild-type adenoviruses of other serotypesHendrik J Rademaker
Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
J Gen Virol 83:1311-4. 2002..To prevent vector mobilization by wt Ads, new vectors should be developed in which essential functions such as the initiation of DNA replication and genome packaging are restricted...
- A new type of adenovirus vector that utilizes homologous recombination to achieve tumor-specific replicationKathrin Bernt
Division of Medical Genetics, University of Washington, Seattle, Washington 98195, USA
J Virol 76:10994-1002. 2002..These new Ad.IR-E1A vectors combine elements that allow for tumor-specific transgene expression, efficient viral replication, and spread in liver metastases after systemic vector application...
- Construction and characterization of E1-minus replication-defective adenovirus vectors that express E3 proteins from the E1 regionKaroly Toth
Department of Molecular Microbiology and Immunology, Saint Louis Unversity School of Medicine, Saint Louis, Missouri 63104, USA
Virology 301:99-108. 2002..7K, as expected. The RID-expressing vectors forced the internalization and degradation of Fas and EGFR, and they inhibited apoptosis induced through the Fas pathway. These vectors should be useful reagents to study the E3 proteins...
- Characterization of bovine adenovirus type 3 E1 proteins and isolation of E1-expressing cell linesAlberto L van Olphen
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana, 47907, USA
Virology 295:108-18. 2002..These cell lines will be useful in generating and growing BAV3 E1-deleted recombinants, and also for studying E1 protein interactions with a number of cellular and/or viral proteins...
- Degenerated pIX-IVa2 adenoviral vector sequences lowers reacquisition of the E1 genes during virus amplification in 293 cellsJ J Robert
Aventis Gencell-CNRS-IGR UMR 1582, Institut Gustave Roussy, Villejuif, France
Gene Ther 8:1713-20. 2001..This approach could also be applied to decrease reincorporation of the E1 genes during amplification of deltaE1deltaE4 vectors in 293/E4-trans-complementing cells...
- Inhibition of tumor necrosis factor alpha-induced NF-kappa B activation by the adenovirus E3-10.4/14.5K complexJoshua M Friedman
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Virol 76:5515-21. 2002....
- Hepatocarcinoma cells constitutively expressing adenoviral E1-genes provide a tumor model for intratumoral replication of E1-deficient adenovirusesGuenter Cichon
Department of Molecular Cell Biology, Institute for Biology, Humboldt University Berlin at the Max Delbrueck Center for Molecular Medicine, Germany
Anticancer Res 22:197-201. 2002..Moreover, Huh7-E1 tumors release human alpha-1-antitrypsin (hAAT), which allows the monitoring of occult growing tumors (i.e. liver, peritoneum) by measuring serum hAAT levels...
- Transcription initiation activity of adenovirus left-end sequence in adenovirus vectors with e1 deletedMasato Yamamoto
Division of Human Gene Therapy, Department of Medicine, and the Gene Therapy Center, University of Alabama at Birmingham, 35294 2172, USA
J Virol 77:1633-7. 2003..Further understanding of the transcription initiation activity of the left-end sequence of the adenovirus genome should lead to optimization of adenovirus vectors...
- A single short stretch of homology between adenoviral vector and packaging cell line can give rise to cytopathic effect-inducing, helper-dependent E1-positive particlesPete Murakami
Berlex Biosciences, Process Development Department, Richmond, CA 94804, USA
Hum Gene Ther 13:909-20. 2002..C6 (lacking the 177 nucleotide region of homology) has allowed extensive virus propagation (Ad5.1FGF-4) with no CPE- or HDEP-like events yet detected...
- Telomerase-specific oncolytic virotherapy for human cancer with the hTERT promoterToshiyoshi Fujiwara
Center for Gene and Cell Therapy, Okayama University Hospital, Department of Surgery, Okayama Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
Uirusu 58:11-8. 2008..This article reviews recent highlights in this rapidly evolving field: cancer therapeutic and cancer diagnostic approaches using the telomerase-specific oncolytic adenoviruses...
Research Grants79
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2010..These studies will lead to improved strategies for re-establishing immune privilege of corneal transplants and offsetting the untoward effects of allergic diseases on the fate of corneal transplants. ..
- IMMUNOLOGICAL MODULATION OF OCULAR TUMOR METASTASESJerry Niederkorn; Fiscal Year: 2005..The long rang goal of this project is to design, develop, and evaluate novel immunotherapeutic modalities for the prevention and treatment of uveal melanoma metastases. ..
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2004..All three of these aims will be addressed in a well-characterized mouse model of penetrating keratoplasty. ..
- Prime-boost regimens for adenoviral vaccines to HIV-1Hildegund Ertl; Fiscal Year: 2004..abstract_text> ..
- E1-deleted adenoviral vectors for oral immunizationHildegund Ertl; Fiscal Year: 2004..In aim 4, we will determine the tropism of the simian and human Ad recombinants upon oral application emphasizing localization of and cytokine production by transduced antigen presenting cells (APCs). ..
- Oral Vaccine to Inhalation RabiesHildegund Ertl; Fiscal Year: 2004..In aim 2, the AdC68rab.gp vaccine will be tested in a non-human primate rabies virus challenge model. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2003..g., corneal transplantation). In some cases, it may be desirable to abrogate immune privilege to promote the immune-rejection of sight- and life-threatening ocular neoplasms. ..
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2002..All three of these aims will be addressed in a well-characterized mouse model of penetrating keratoplasty. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2002..g., corneal transplantation) or abrogate immune privilege to protect against sight- and life-threatening neoplasms. ..
- IMMUNOLOGICAL MODULATION OF OCULAR TUMOR METASTASESJerry Niederkorn; Fiscal Year: 2002..The long rang goal of this project is to design, develop, and evaluate novel immunotherapeutic modalities for the prevention and treatment of uveal melanoma metastases. ..
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2001..Both strategies procure antigen-specific suppression of host immune responses and should prove useful in preventing corneal graft rejection. ..
- CANCER VACCINE TO P53Hildegund Ertl; Fiscal Year: 2001....
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2008..These studies will lead to improved strategies for re-establishing immune privilege of corneal transplants and offsetting the untoward effects of allergic diseases on the fate of corneal transplants. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2001..g., corneal transplantation) or abrogate immune privilege to protect against sight- and life-threatening neoplasms. ..
- CANCER VACCINE TO P53Hildegund Ertl; Fiscal Year: 2000....
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2006..g., corneal transplantation). In some cases, it may be desirable to abrogate immune privilege to promote the immune-rejection of sight- and life-threatening ocular neoplasms. ..
- Oral Vaccine to Inhalation RabiesHildegund Ertl; Fiscal Year: 2006..In aim 2, the AdC68rab.gp vaccine will be tested in a non-human primate rabies virus challenge model. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2010....
- IMMUNOLOGICAL MODULATION OF OCULAR TUMOR METASTASESJerry Niederkorn; Fiscal Year: 2010..The fourth aim will evaluate the therapeutic efficacy of a monoclonal antibody directed against human CD54 in controlling liver metastases in a nude mouse model of uveal melanoma. ..
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2009..These studies will lead to improved strategies for re-establishing immune privilege of corneal transplants and offsetting the untoward effects of allergic diseases on the fate of corneal transplants. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2009....
- IMMUNOLOGICAL MODULATION OF OCULAR TUMOR METASTASESJerry Niederkorn; Fiscal Year: 2009..The fourth aim will evaluate the therapeutic efficacy of a monoclonal antibody directed against human CD54 in controlling liver metastases in a nude mouse model of uveal melanoma. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2008..abstract_text> ..
- HIV-1 Vaccine Based on Chimp Serotypes of AdenovirusHildegund Ertl; Fiscal Year: 2008..Studies by HVTN will be complemented by studies of Project 3, which will assess in human volunteers the quality of vaccine-induced gag-specific T cell responses in relationship to pre-existing immunity to the vaccine carrier. ..
- Immune Responses To AAV-Mediated FIX Gene TransferHildegund Ertl; Fiscal Year: 2008..The projects will be supported by an Administrative Core, which will provide the needed oversight, and a Vector Core, which will provide the investigators with purified and quality controlled vectors. ..
- INFRASTRUCTIRE DEVELOPMENT GRANT FOR CORNEA RESEARCHJerry Niederkorn; Fiscal Year: 2008..These facilities will save time, energy, and resources while enhancing the success of seven NEI-sponsored research programs at U.T. Southwestern Medical Center. ..
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2007..These studies will lead to improved strategies for re-establishing immune privilege of corneal transplants and offsetting the untoward effects of allergic diseases on the fate of corneal transplants. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2007....
- Immune Responses To AAV-Mediated FIX Gene TransferHildegund Ertl; Fiscal Year: 2007..The projects will be supported by an Administrative Core, which will provide the needed oversight, and a Vector Core, which will provide the investigators with purified and quality controlled vectors. ..
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 2006..All three of these aims will be addressed in a well-characterized mouse model of penetrating keratoplasty. ..
- Genetic Vaccine to Rabies VirusHildegund Ertl; Fiscal Year: 2006..In aim 3, we are proposing experiments in non-human primates to test the vaccine for efficacy in pre- and post-challenge vaccination experiments with and without concomitant application of RIG. [unreadable] [unreadable]..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 2000..g., corneal transplantation) or abrogate immune privilege to protect against sight- and life-threatening neoplasms. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 1993..The long range goals of this project are to understand immune regulatory processes in the eye and to manipulate such processes to reduce immune-mediated injury and corneal graft rejection...
- IMMUNOLOGICAL MODULATION OF OCULAR TUMOR METASTASISJerry Niederkorn; Fiscal Year: 1992..The long range goal of this project is to implement the optimal protocols for each step of the metastatic process into a single comprehensive plan for preventing and treating metastases arising from intraocular melanomas...
- CANCER VACCINES TO HPV-16 ASSOCIATED TUMORHildegund Ertl; Fiscal Year: 1999..In case the applicant's hypothesis is correct, data gathered within the realm of this application will serve as the basis for a clinical Phase I trial. ..
- TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYEJerry Niederkorn; Fiscal Year: 1990..Understanding immune privilege may lead to strategies for promoting long term survival of therapeutic organ grafts...
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 1992
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 1991
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 1990
- IMMUNOBIOLOGY OF CORNEAL ALLOGRAFTSJerry Niederkorn; Fiscal Year: 1993..The results from these investigations should provide important insights into the immunobiology of corneal allografts and the feasibility of specific immunological strategies for promoting corneal allograft survival...
- CANCER VACCINES TO HPV-16 ASSOCIATED TUMORHildegund Ertl; Fiscal Year: 2000..In case the applicant's hypothesis is correct, data gathered within the realm of this application will serve as the basis for a clinical Phase I trial. ..
- HIV-1 Vaccine-Induced CD8+ Memory CellsHildegund Ertl; Fiscal Year: 2003..The role of the two different memory CD8+ T cell populations in limiting propagation of the vaccinia gag recombinant virus will be tested by adoptive transfer experiments. ..
- CANCER VACCINES TO HPV-16 ASSOCIATED TUMORHildegund Ertl; Fiscal Year: 2001..In case the applicant's hypothesis is correct, data gathered within the realm of this application will serve as the basis for a clinical Phase I trial. ..
- CANCER VACCINE TO P53Hildegund Ertl; Fiscal Year: 1999....
- TRAINING PROGRAM IN THE IMMUNOBIOLOGY OF VACCINESHildegund Ertl; Fiscal Year: 2007..All of the course work will take place at The Wistar Institute. ..
- Oral Vaccine to Inhalation RabiesHildegund Ertl; Fiscal Year: 2005..In aim 2, the AdC68rab.gp vaccine will be tested in a non-human primate rabies virus challenge model. ..
- Mucosal Vaccine to HIV-1 GagHildegund Ertl; Fiscal Year: 2002..In the third aim, we will test a prime-boost regimen using DNA vaccines, expressing Gag for systemic priming combined with the adenoviral recombinants for systemic or intranasal booster immunization. ..


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Yingbin Hotel is a very typical Chinese hotel. One the plus side, the room I had was quite large with a good size bathroom. There was plenty of hot water and good water pressure even up on the 12th floor. The room was comfortably appointed with a queen sized bed, a desk for working and a comfortable armchair by the... More
Location: China > Guangdong > Lechang
- Also Known As:
- Yingbin Hotel China/Lechang


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You're leaving Wedding Paper Divas to see the Deal of the Day at our parent site, Tiny Prints.
You won't be able to dismiss this by usual means (escape or click button), but you can close it programatically based on user choices or actions.
"I used these with the blue background for a winter wedding and they were beautiful! Everyone commented on how beautiful the invitation was!"
"These were absolutely beautiful and we're complimented by many. The paper was a nice weight, and thermography gave them a classy touch. My only complaint was, they wre about a quarter if an inch too tall, so I had to pay for oversized shipping. I still think the result was worth the extra few cents shipping."
"I ordered my cards after completing the design. I made further changes and nothing was too much trouble, the amendments were made without fuss. The paper quality of the invitations and the envelopes were excellent. They even came with a tissue insert for the envelope and an extra 7 cards as spares. Service was exceptional and delivery very quick Will definitely be recommending to friends and family."
"I purchased these invitations, but had them changed from a wedding invitation to an engagement invitation. I was also able to have a line added so that I could write people's names in. They turned out just perfect. Everybody loved them. I will defiantly be using wedding paper divas for my wedding invitations etc."
"WOW is all I continue to say. I am so unbelievably happy with the final product of my invitations. I had ordered several samples and this one stuck out immediately! I loved the design and the gold color on the creme stock card. Although there was other color options when ordering, I kept the gold as I thought it was very classy and appropriate for our venue. I had a couple of designer requests made to the invite so I was anxious to see how it would finally look in person. It came out awesome! I had requested the grooms parents names be written under his and that "black tie optional" be added to the bottom right. My fiance and I are so very happy with the way they came out and I cannot wait to send them out to all my guests. We also had the return addresses printed on all the envelopes as well as a matching gold liner added to the envelopes."


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Keep up to three users safe and sound on the internet without the worry of virus or malware infection with McAfee Internet Security 2014, and get the most out of the web.
Protecting your data
Whether you want to protect your favourite photos, tax records or something else, you can back them up to protected online storage. It is password protected and encrypted, so you can be sure your data is safe. The intuitive touch screen can be accessed just as easily with trackpad and mouse or with touch controls so navigating your security options stays nice and easy, whatever system you are using.
Spam and scam
If you're fed up with your email inbox filling up with junk mail, Internet Security 2014 could be the answer to your problems. Spam filters help to stop you from receiving intrusive attachments and links that may allow viruses and worms to access your PC.
Advanced spam filters and active analysis help to protect your PC against being spammed.
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If you spend your life on your PC, you wouldn't want to see anything bad happen to it, which is where Internet Security 2014 can help. It helps to protect your PC from common problems such as viruses, spyware, malware and Trojans that try to sneak their way onto your system.
It works in real time, so threats are blocked as soon as they rear their head on your system.
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It can be difficult to monitor what your children have access to online, but with Internet Security 2014 you can set rules that judge the age range and category of the page so your kids can use the internet safely.
Text alerts can keep you informed if someone tries to access a restricted site as well as set time limits that allow you to limit the time your children spend on the internet and protecting them with extensive site and social media filtering while they are online.
Use McAfee Internet Security 2014 for the best way to keep your whole family protected online.
NB: Please note that this is an activation key only and you will need to download the software. A CD is not provided.
Product code: 074591


text:
My Profile
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Flag This ProfileFlag as Inappropriate
Reviewed Carrot Cake Cupcakes
Sep 24, 2011 in Food Network Community Toolbox on FoodNetwork.com
"I just finished these cupcakes and they came out PERFECT! I cooked them in a convection oven at 350, for just over 23 minutes (when the toothpick came off clear) I didn't use walnuts, and golden raisins... They were so moist and i will definitely flag it for future use! """
Reviewed Spaghetti alla Carbonara
Apr 3, 2011 in Food Network Community Toolbox on FoodNetwork.com
"Our best meal yet!! This carbonara recipie was easy, and very tasty. I added mushrooms and baked chicken to the recipe and was absolutly delicious!""


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Three Charts to Send to Your Right-Wing Relatives, Guaranteed to Make Their Heads Spin
Problem: Your right-wing relative is plugged into the Fox-Limbaugh lie machine, and keeps sending you emails about "Obama spending" and "Obama deficits" and how the "stimulus" just made things worse.
Solution: Here are three "reality-based" charts to send to him. These charts show what actually happened.
Government spending increased dramatically under President Bush. It has not increased much under President Obama. This is just a fact.
Note that this chart starts with Clinton's last budget year for comparison.
The numbers in these two charts come from Budget of the United States Government: Historical Tables Fiscal Year 2015. They are just the amounts that the government spent and borrowed, period, Anyone can go look them up. People who claim that Obama "tripled the deficit" or increased it or anything of the sort are either misled or are trying to mislead. President Obama inherited a budget deficit of $1.4 trillion from President Bush's last budget year and annual budget deficits have gone down dramatically since.
The Stimulus and Jobs
In this chart, the RED lines on the left side – the ones that keep doing DOWN – show what happened to jobs under the policies of Bush and the Republicans. We were losing lots and lots of jobs every month, and it was getting worse and worse. The BLUE lines – the ones that just go UP – show what happened to jobs when the stimulus was in effect. We stopped losing jobs and started gaining jobs, and it was getting better and better.
The leveling off on the right side of the chart shows what happened as the stimulus started to wind down: job creation leveled off at too low a level.
It looks a lot like the stimulus reversed what was going on before the stimulus. We have gone from losing around 850,000 jobs a month to gaining over 200,000 jobs a month.
Conclusion: THE STIMULUS WORKED BUT WAS NOT ENOUGH!
More False Things
These are just three of the false things that everyone "knows" because places like Fox News repeat them over and over and over. Some others are ( click through): Obama bailed out the banks, businesses will hire if they get tax cuts, healthcare reform cost $1 trillion, Social Security is a Ponzi Scheme or is "going broke", tax cuts grow the economy, government spending " takes money out of the economy."
Actually, This Reduced Spending And Lower Deficit Have Hurt The Economy
Government spending is literally, by definition, the things that government does to make our lives better. People have been tricked into thinking that government spending is somehow bad. The billionaires and giant corporations spread this nonsense around because they are greedy and just want their taxes lower. The top income tax rate used to be more than 90 percent and the top corporate tax rate used to be more than 50 percent. That was back when we built this country's great infrastructure, had good schools and defended the world against the Soviet Union. We also had higher economic growth and a growing middle class.
Government spending does not "take money out of the economy." In fact it puts money into the economy, creates jobs and lays the foundation for future prosperity. The decline in government spending shown in the charts above is the reason that the economy remains sluggish and jobs are still hard to get. Just look at that chart showing what the stimulus spending did for the job situation. But since the stimulus ended, Republicans have obstructed every effort to continue to use our government to help our economy.


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Milan's designer label food
The Milanese have taken their flair for design and haute couture and relocated it to the kitchen.
Read more… 19 October 2012
The Daily Meal unveiled its list of the "most exemplary epicurean endeavours on the European continent", featuring restaurants in 26 countries and 70 cities.
Read more… 21 December 2012
The Milanese have taken their flair for design and haute couture and relocated it to the kitchen.
Read more… 19 October 2012
Change can be slow to come to the Eternal City, where the best version of something is often the most ancient. But the chefs behind these five restaurants dare to mess with tradition.
14 June 2012
The wackiest eateries around the globe replicate the in-flight experience, replace tables with toilets and serve dinner in a prison.
29 May 2012
Seven perfect destinations for the best food without a face – and three for vegetarians to avoid.
Read more… 23 April 2012
Italy and France may have brought fame and fortune to the truffle, but this rare mushroom can actually be found all over the world if you know where to look.
Read more… 23 March 2012
This region has an impressive share of art and history, but it is the local traditions and way of life that make this the slow food and slow travel capital of Italy.
Read more… 21 October 2011
Succulent seafood and rustic, farm fresh dishes are standard fare in northeast Sardinia. Eat, drink, play and stay on any budget on this idyllic island coast.
Read more… 04 October 2011
The tradition of pre-dinner drinks served with snacks has morphed into a nightly phenomenon of complimentary lavish buffet spreads.
Read more… 22 September 2011
Famed for their food, Tuscany serves up dish after dish of earthy delights.
12 September 2011
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Border State Electric's CEO Tammy Miller laughed while meeting with employees at the company's Albertville location during a visit Thursday, Oct. 25, 2012, at Border States Electric in Albertville, MN.
Photo: David Joles, Star Tribune
Title: CEO, Border States Electric
Home: Fargo, N.D.
Family: Spouse, Craig Palmer
Age: 52
Education: Bachelor's degree in accounting (1982) and MBA (1996) from Minnesota State University, Moorhead
Career: Certified public accountant with regional accounting firm 1982-1990; joined Border States January 1991 as accounting manager, held other positions, including controller, vice president of finance, general manager of the southwest region in Phoenix, executive vice president and president. CEO since January 2006.
Hobbies: Running, reading, travel, volunteer work


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Last week, HBO aired "The Case Against 8," kicking off what will become a weekly event on HBO: Monday Night Documentaries. Tonight, HBO will follow up with Doug Block's "112 Weddings" at 9:00 ET as Doc Night gets going.Read More »
The acclaimed documentary series, which chronicles the search for a first-time director as well as exploring the process of getting films made, is coming back for a new season.Read More »
While further study on the legendary rock 'n roller is needed, this search for his motivation is based in the wrong arena.Read More »
Directors Kate Davis and David Heilbroner will have their film showcased at the Tribeca Film Festival April 20th before debuting on HBO in July.Read More »
Director Edgar Barens spent six months at Iowa State Penitentiary, a maximum security prison in order to film the HBO documentary "Prison Terminal: The Last Days of Private Jack Hall," which was nominated for an Academy Award for Best Documentary Short Subject.Read More »
Sam Berns, the 17-year-old Massachusetts high school student whose life with the rare illness progeria was the subject of the documentary "Life According to Sam," died on Friday, The New York Times reports.Read More »
Just a day after newly freed Pussy Riot members Nadezhda Tolokonnikova and Maria Alyokhina announced the disbandment of Russia's most important feminist punk group, the Putin administration canceled the first public screening of the film "Pussy Riot: A Punk Prayer."Read More »
An in-depth interview with documentary filmmaker Dawn Porter, whose latest film, 'Gideon's Army,' is featured at DOC NYC.Read More »
Susan Lacy is leaving her long-time spot at WNET to work with Sheila Nevins at HBO Documentary Films.Read More »


text: [embed width="620px"]http://youtu.be/YHMIfUDBWpE [/embed] About the Summer Arts Intensive Days and Time: July 14–18, 2014 | 11am – 4pm Location: Cal Shakes Theater in Orinda, CA. We will provide a *free* shuttle between Orinda BART & the venue for all participants. Cost: Free Youth Speaks' Summer Arts Intensive is a week-long multidisciplinary, arts camp for Bay Area youth ages 13-19. Through interactive workshops and a lecture-style speaker series featuring world-renowned social justice scholars, spoken word performers, theater and movement experts, you'll explore topics like: ethnic studies, political education, documentary storytelling, critical media literacy, movement/dance and various elements of Hip Hop. The Intensive is a rigorous growth community where each participant is challenged to IMAGINE a world where giving VOICE to our stories is a daily act of courage. Deadline to apply is: Friday, June 13, 2014. Those invited to participate in the Summer Program will be notified via email by Friday, June 27, 2014. Preference is given to students able to attend the full week. [wpgform id='4699']


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WHAM13 - Search Results
Job applications online for new Rochester mayor
Rochester, N.Y. - Mayor-elect Lovely Warren is accepting job applications.
Starting Monday, anyone interested in working at City Hall when the new Warren administration takes over in January, can apply online.
Job seekers can go to warrentransition.com and upload a resume and cover letter.
Make sure to include what position you're interested in, and why you would be an asset to the Warren administration.


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The Home Depot is the world's largest home improvement retailer and second largest retailer in the United States. We help our customers build their dreams by being more than a store.
Instructions: Click on the "Position Details" links to see more and Apply Now!
|Locations having this position available...
|256-260 Kingston Road East - Ajax, ON L1S4S7
|Position Details
→ Criteria: State:ON>City:Ajax
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