text:
Can't see files on the PSP when connected to PC
This topic is locked from further discussion.
Hello there. So I borrowed this psp from my friend. And I want to install a game there. I already download a game in my PC. Now, I'm gonna install it. So I connected the usb to my pc. Then I opened the F drive, there are folders there like, registry, gps, etc. But I can't find the files that are installed in the PSP. The psps has songs, games, but you can't see it on the F drive on my PC. And the "show hidden folders" is enabled so that's all the files there. And when I looked at the total size of that F drive, it's only 4Kb. In which the memory card is 4Gb. Help? kielmaruRegistry? GPS? Those aren't regular PSP folders... All I can say is to either update your firmware or get a better USB cable.
Ahhhhh I know what this is, but further comment would get me moderated. :P What you're doing is illegal, you can't just download games online that you don't own.GranpireWait what? What's illegal? So basically, looking at the files on your psp through the computer is illegal? And no, I didn't say that I'm downloading games online. I just want to see the files in the psp.
When he says "illegal" he is not referring to downloading games. It's what's going on in the PSP that's illegal. Like he said, we'd be in trouble if we talk about it here. (Geez, why does every other thread in here have to deal with it anyway?) But allow me to put it in context. Your friend has installed "something" to the PSP itself, and used that "something" to tell the PSP to read the area of the PSP where that "something" is installed rather than the Memory Stick when it's plugged in to the computer. Probably for his own devious reasons. That's why the computer can't read the Memory Stick and/or why you're only seeing 4KB on whatever it does read.tjoeb123
Actually, the opposite is true. What your friend has done to his PSP is a perfectly legal modification to his system. It's downloading games that's illegal. And there's nothing "devious" about the accessing the PSP's flash. Usually, it's done to modify themes or system colours.
Wait what? What's illegal? So basically, looking at the files on your psp through the computer is illegal? And no, I didn't say that I'm downloading games online. I just want to see the files in the psp. kielmaru
Yes, in the first post you said "I downloaded a game in my PC" This means you either downloaded the game online, or you got it from a hard drive or some other storage. Either way, it's illegal unless your friend bought the game from a store, and your trying to download games onto your PC only kind of reinforces that you're trying to play an ISO rip of a game.
Quick NavigationJump to Top
Please Log In to post.


text:
- Cooking Time:
- Servings: 30 to 35
- Preparation Time:
- 1 Egg
- 1 cup Sugar
- 1 1/2 tbl Melted lard or butter
- 1 cup Sour milk, or buttermilk
- 1 tsp Grated nutmeg
- 4 cup Flour
- 1 1/2 tsp Baking soda
- 2 tsp Cream of tartar
- 1 tsp Salt
- Beat the eggs and sugar together.
- Beat in the lard and then the sour milk or buttermilk.
- Sift the dry ingredients together.
- Add to the egg mixture and mix well.
- Roll the dough out on a floured board to a thickness of 1/4 inch.
- Cut into rings with a doughnut cutter.
- Let the rings rest for 10 to 15 minutes before cooking them.
- Fry in deep hot oil until brown on both sides. Remove with a slotted spoon and drain on paper towels then roll in sugar or a mixture of cinnamon and sugar.
Presented by
Holiday Meal Planning
Wine Pairing Chat
Cupcake Recipes: Allison Robicelli Cupcake Chat
Get Fit With Good Food!
Food Travels Through Italy
$30 Wine+Food PairingsSee More
Citrus-Roasted Chicken
Hard Rock Cafe Cole Slaw Clone
Mashed Sweet PotatoesSee More

subset: commoncrawl


text:
The Leading eBooks Store Online
for Kindle Fire, Apple, Android, Nook, Kobo, PC, Mac, BlackBerry...
New to eBooks.com?Learn more
- Bestsellers - This Week
- Foreign Language Study
- Pets
- Bestsellers - Last 6 months
- Games
- Philosophy
- Archaeology
- Gardening
- Photography
- Architecture
- Graphic Books
- Poetry
- Art
- Health & Fitness
- Political Science
- Biography & Autobiography
- History
- Psychology & Psychiatry
- Body Mind & Spirit
- House & Home
- Reference
- Business & Economics
- Humor
- Religion
- Children's & Young Adult Fiction
- Juvenile Nonfiction
- Romance
- Computers
- Language Arts & Disciplines
- Science
- Crafts & Hobbies
- Law
- Science Fiction
- Current Events
- Literary Collections
- Self-Help
- Drama
- Literary Criticism
- Sex
- Education
- Literary Fiction
- Social Science
- The Environment
- Mathematics
- Sports & Recreation
- Family & Relationships
- Media
- Study Aids
- Fantasy
- Medical
- Technology
- Fiction
- Music
- Transportation
- Folklore & Mythology
- Nature
- Travel
- Food and Wine
- Performing Arts
- True Crime
- Foreign Language Books
Most popular at the top
The Pre-Dreadnought RevolutionThe History Press 2013; US$ 24.78
The late nineteenth century saw an unparalleled revolution in warship development as the Victorian navy found itself grappling with intense technical change to ensure its survival in the modern theatre. From the wooden battleships of the 1800s, naval architecture underwent great change to produce a very different form of capital ship, which would... more...
TrainDK Publishing 2014; US$ 40.00
Train is a glorious celebration of all things train and track, tracing the history of the railroad and the role of trains, from the first steam engines to today's high-speed bullet trains. Featuring DK 's trademark brilliant photography, Train catalogs the development of trains from early steam to diesel engines and electric locomotives, explores... more...
The Train BookDorling Kindersley Ltd 2014; Not Available
Definite visual history and glorious celebration of all things train and track The Train Book traces the history and role of trains from the first steam engines to diesel engines and then to today's high-speed bullet trains. You'll take a journey through the most important trains of each period and see iconic trains such as the Orient... more...
The RailwayWharncliffe 2014; US$ 39.99
Never before has a comprehensive history been written of the track used by railways of all gauges, tramways, and cliff railways, in Great Britain. And yet it was the development of track, every bit as much as the development of the locomotive, that has allowed our railways to provide an extraordinarily wide range of services. Without the track of today,... more...
Railway AccidentsOsprey Publishing 2014; US$ 7.95
Though trains are one of the safest forms of transport, train accidents always make headline news. Their history is, in many ways, the history of technological development and learning. Early incidents like the felling of William Huskisson MP by Stephenson's Rocket in 1830 led to the reporting systems we know today, while within 50 years safer signalling... more...
The Old Patagonian ExpressHoughton Mifflin Harcourt 2014; US$ 15.95
Starting with a rush-hour subway ride to South Station in Boston to catch the Lake Shore Limited to Chicago, Theroux winds up on the poky, wandering Old Patagonian Express steam engine, which comes to a halt in a desolate land of cracked hills and thorn bushes. But with Theroux the view along the way is what matters: the monologuing Mr. Thornberry... more...
Hook and EyeUniversity of Minnesota Press 2001; US$ 35.00
Analyzing the origins, growth, and eventual dismantling of the Iowa Central Railway, Don L. Hofsommer examines how this plain vanilla railway was an example of the life cycle of the American railroad industry. Drawing the story from station records, ann more...
Advanced Train Control SystemsWIT Press 2010; US$ 134.00
Advanced Train Control Systems are playing an important role in improving the efficiency and safety of train operation, acting as their brains and nerves. ATCS needs highly reliable and safe systems using complex computer tools. Normally, these systems consist of the four parts: the central control system; the station control systems... more...
The Flying ScotsmanOsprey Publishing 2011; US$ 9.95
Flying Scotsman is probably the most famous railway locomotive in the world. When it was new in 1923 it caused a sensation for its beauty and its speed, and the engine found itself at the center of media attention that continued throughout the decade and made it a household name - to see it was the ambition of every young trainspotter. But The Flying... more...
DerailedSt. Martin's Press 1997; US$ 6.99
America needs train service. It suffers from crowded highways and airports, making travel nearly intolerable. Amtrak's future is bleak, and Congress is demanding that Amtrak be profitable by the turn of the century or shut down. Joseph Vranich, who worked to create Amtrak, now nearly three decades later declares it a "failed experiment." Free of... more...


text:
- Shopping Bag ( 0 items )
Posted December 3, 2011
It spends more time informing you of this or that and less time offering helpful tips ( which are few and far between). Too much history. Too much personal opinion. Was not helpful to me at all. Do not waste your money.Was this review helpful? Yes NoThank you for your feedback. Report this reviewThank you, this review has been flagged.


text:
A Growing Baby Bump Won't Keep Kim K Out of Lingerie (PHOTOS)
Kim Kardashian opts for sexy wear, rather than maternity wear, on a recent shopping trip.
Just because there's a bun in the oven, doesn't mean a lady can't look sexy.
Though she's expecting her first child with beau Kanye West this summer, Kim Kardashian isn't drowning her famous curves in dowdy maternity wear — at least, not yet. Dressed in a conservative blue suit, the E! reality star eyed some lacy undergarments just ahead of Valentine's Day while shopping in Beverly Hills on Tuesday.
Now four months along, the Keeping Up With the Kardashians star, 32, is showing just a glimmer of a belly. And while her fashions may soon have to give way to her blossoming baby bump, Kardashian is apparently still keeping things spicy for beau Kanye West while she still can.
At Robertson Boulevard's Curve boutique, Kardashian checked out a racy white-lace bralette and a low-cut corset while scanning the racks.
The shopping spree came on the heels of an afternoon meeting with her lawyer Laura Wasser, who is handling her divorce from estranged husband Kris Humphries.schedule. But it won't be long before she'll have to ditch her racy digs for a more conservative maternity wear.
In the meantime, we applaud Kim for keeping her sex-pot dream alive.
How do you guys feel? Should Kim be shopping for lingerie or for maternity wear and baby goodies? Tell us below.


text:
T.J. Yates suffers bruised shoulder
Updated: January 2, 2012, 2:28 AM ETESPN.com news services
Texans quarterback T.J. Yates left Sunday's 23-22 loss to Tennessee in the first quarter after landing on his left shoulder following a hard hit.
Coach Gary Kubiak said Yates had a bruised left shoulder but could've returned if necessary. Kubiak also said Yates would undergo more tests Monday.
"Obviously, I didn't want him to go back in the game," Kubiak said. "We'll know better where we're at (Monday), at this point."
Yates was replaced by veteran journeyman Jake Delhomme.
The ...
Read full story
You must be signed in to post a comment
You are fully responsible for the content you post. Content that includes profanity, personal attacks or antisocial behavior (such as "spamming" or "trolling"), or other inappropriate content or material will be removed. We reserve the right to block any user who violates our terms of use, including removing all content posted by that user.
- Kuharsky: Reaction to Bill Polian firing
- Joyner: Colts can be contenders soon
- Yasinskas: Next coach for Bucs?
- Sando: A plan for St. Louis
BENGALS AT TEXANS (Sat., 4:30 ET)
LIONS AT SAINTS (Sat., 8 ET)
FALCONS AT GIANTS (Sun., 1 ET)
BRONCOS AT STEELERS (Sun., 4:30 ET)
- Williamson: Broncos stumble into playoffs
- Joyner: Don't count the Steelers out
- Outsiders: Tim Tebow's last stand
- Matchup page
COWBOYS AT REDSKINS (Sun., 8:20 ET)
- Tucker: Allen, MJD command respect
- Ashley Fox: Between the Tackles in Week 17
- Scouts Buzz: Comeback Pats, more
- Outsiders: Week 17 highs/lows
- Schefter: Coaching hot seat updates
- John Clayton: AFC playoff Q&A
- Jeffri Chadiha: NFC playoff Q&A
- Hensley: Super Bowl run hinges on Ray Rice
- Walker: Jets are stuck with Sanchez
- Week 17 statistical leaders


text:
Very good place, near the Sequoia National park as well as few restaurants. The room is large, everything was clean and we had access to a small fitness room, sauna and outside swimming pool! You even have a free continental breakfast included in the price. This hotel is worth it's price.
Location: United States > California > Three Rivers
Ranked #2 of 10 Specialty Lodging in Three Rivers
- Official Description (provided by the hotel):
- A private campground offering two RV parks. ... more less


text:
SmK - Make A Wish
Credits & Info
- Date
- 01/20/2013
- File Info
- Song
- 3 min 9 sec
- Score
- 4.39 / 5.00
Related Content
You might also enjoy:
Licensing Terms
- Attribution:
- You must give credit to the artist.
- Noncommercial:
- You may not use this work for commercial purposes unless you make specific arrangements with the artist.
- Share Alike:
- If you alter, transform, or build upon this image, you may distribute the resulting creation only under a license identical to this one.
- Score:
- Rated 4.39 / 5 stars
- Plays & Downloads:
- 4,897 Plays | 757 Downloads
- Share Links:
- Genres:
- Electronic - Trance
- dream
- trance
- skillzmakekillz
- smk
Author Comments
Rated 5 / 5 stars
Absolutely beautiful.
Your music makes me wanna cry.
but i never cry so I'm not gonna.
Anyway, this seriously made my night
faved you and this song easily.
great work.
Rated 5 / 5 stars
WOW Just WOW That is amazing. Good job!
Rated 4.5 / 5 stars
I love the melodies! I imagine that this would go great with some lyrics. I was singing along just saying "Make A Wish" haha. I agree with jakeghost, it sounds like this song was made for his kind of voice. Great job! ^__^
thanks! :D
Rated 3.5 / 5 stars
This reminds me a bunch of Owl City! Good work on your choice and use of synths. the big bits sounded big and the softer bits sounded soft. :)
Rated 5 / 5 stars
Thanks for all the upbeat great tunes, definitely made my miserable days into cheerful ones... I wish you best in your future career and endeavors. We will sorely miss you here at NG.
With love, your fan,


text:
Added Sep 3, 2012
by KazztheSpazz
Melbourne, Austr...
This dress was made for the Sew weekly Book Challenge, channelling Weetzie Bat and self drafting the Multi Coloured Versus Cut-Out Dress. More photos and details on the Blog.
Broad Cloth and low sheen satin.
Sep 4, 2012, 09.21 PMby Gabriela Alejandra
wow!!! geometric rainbow!!!!!!!!
Sep 3, 2012, 09.40 AMby Mary Athey
Amazing dress and what a great use of colour. just fab xx
Sep 3, 2012, 10.40 AMby KazztheSpazz
Thank you so much Mary.
Add to Favorites no one added it
Pattern of the Week
The oversized shape has a cool bell shaped silhouette with a wide body and fitted sleeves
Editor's Pick
Stock up on our favorite BurdaStyle patterns sewn in white!
Web Seminar
Learn How to Sew Knits Successfully With a Sewing Machine
Online Course
Join Meg Healy in this 6 Week Course, we have just re-opened enrollment for a limited time!
Meg's Magazine Mash Up 2014
Take a look at Meg's 2014 Mash Ups, and get the special pattern bundle that includes them all
You must allow our "request for permission" request to login to Burdastyle with Facebook.


text:
Ira Twp, Michigan
My name is Jenna and on October 17, 2008 I was involved in a rather serious auto accident. My truck was totalled and I was not in much better shape. Shortly after I began developing an excrutiating pain in my right leg... almost two months later a 19cm. hematoma was found in my thigh, unfortunately, it was found much to late. By that time it had abcessed and I developed two severe infections. I underwent two surgeries, spent weeks in the hospital, was sent home with an IV that I was forced to... Full Bio


text:
B&B Montelimar
No availability found through our partners. Please contact the business directly or check some of our recommended alternatives.
Photos of B&B Montelimar
Travelers also recommend:
TripAdvisor Reviews B&B Montelimar Les Tourrettes
Travel Blogs from Les Tourrettes
Sarah returns
... or teacher....felt v proud of ouselves!
Wonderful to return. The Ardeche Gorge is magnificent, the colours, the light, the amazing views from the road along the top, which David took in his stride driving along (lots of 100 metre sheer drops) whilst I gripped the seat in despair. Conciously I knew the road was a great one, smooth, wide, well marked and with great stopping points ...
Village du chats
... were giddy being so subversive in front of pope palace!
tomorrow we get a walking tour of the city, then at 2p get on a bus to see where Van Gogh painted and some neato light and art show in some caves. I intend to bring both the iPad and iPhone into town in hopes of uploading my photos via wifi that allows more bandwidth (or something). Now we're down to our last 3 days of vacation!
Today is a travel day.
... a great picture from the balcony of our cabin, looking up to the sun deck, hopefully i can get it on the blog. One of the locks we went through had a used condom clinging to the side, I didn't have my camera so I didn't take a picture, but being in public health, I thought it was interesting! After all, I'd rather see a used condom discarded haphazardly than know that someone is having unsafe ...
Finding Our Own Way
... my mind to new ideas about child-rearing that I find both fascinating and incredibly valuable.
I think if I had to come away with just one general conclusion it would be that there really is no right or wrong, no definitive truths about anything, there is only listening to what you feel and experimenting with what works best for your individual self. Life is just one big experiment and we all ...
A Slice of Heaven
... made it spectacular, it was the setting, the generosity with which I was fed, the enjoyment of being able to eat the food at my pace (it was a two hour breakfast), the stimulating and intellectual conversation that followed; it was holistically complete and perfect.
Saturday afternoon Martin took me for a drive to see Vallon and the Pont d'Arc. This is all a part of what is affectionately known as ...


text:
Ever since The One, Huntsville, Alabama duo G-Side has renewed my desire to acquire hood mixtapes just for the whip. Keeping it centered on what they represent, G-Side takes the dirt road to show how keeping knuckles to the ground garners success in the rap game with the free release, Gettin It, featuring Stalley of Maybach Music and Joi Tiffany. The product of a trip that Stalley recently made to Huntsville to record with the team, Gettin It is an orchestrated play on the "come-up" rap track, giving listeners more than G-Side's history: this has everything fans have come to know them for, plus the kitchen sink. Their frequent play on samples, such as the guitar clip from Australian psych rock group Tame Impala, made this Block Beattaz production sheer theatrics. Joi Tiffany's eerie vocals aligned with Stalley's true to life presentation kept this joint supremely…well, cohesive. Whoever would've thought Alabama and Ohio had something in common? Stay with the Booth as we bring more from G-Side with their upcoming, self-titled project, due to drop 11/11/11.
Written by Sigmund'sDope on 09/28/11
Submit your Rating
Slow Motion Soundz
First DJ Booth Appearance:
"Nat Geo" (2011)
You Might Like...
Commentsblog comments powered by Disqus
- Billion Dollars in an Elevator: The Definitive 2014 Hip-Hop Timeline
- DJBooth Announces Our New Top Prospects…
- All 93 People Named on J. Cole's "Note To Self" Outro
- Indie Savage: Crooked I Gets Physical With "Sex, Money & Hip-Hop"
- The Hip-Hop Albums I Need to Hear in 2015
- Meet Fanesha Fabre, the Voice Behind the "La Musica De Harry Fraud" Drop
- 1 Listen Album Review: J. Cole's "2014 Forest Hills Drive" (aka F*cking Up Hip-Hop)
- The Most Sampled Rapper Voices in Hip-Hop History
- Your Favorite Indie Rapper is Secretly Signed to a Major Label
- The DJBooth - Top Prospects EP (Vol. 2)
- The Best Hip-Hop & R&B Songs of 2014 (Ongoing)
DJBooth TV
Discover the best new songs, videos, and albums added to the Booth.


text:
A lovely and dignified lady of the 1870s, she is in excellent condition wearing an early costume. *Early doll collectors called these dolls "Detachable Curls", which is a misnomer due to the fact that the tumbling curls in back were never meant to be removable, they were made in a separate mold for greater detail and permanently attached with plaster or bisque-slip. This unmarked lovely Kling Parian has cafe au lait upswept hair with the tumble of curls in back (a thin manufacturing hairline runs across her forehead and hair-part made when the curls in back were initially attached; her shoulder-plate has also been re-glued). The shoulderhead has three sew holes front & back, and measures 5" tall, 5" across the shoulders, with head circumference of 9". Her features are hand-painted with blue eyes, black & pale red lid lines under long almost-straight brown brows. Rosy blushed cheeks and a tiny slightly smiling mouth are enhanced by the red & white glass bead jewelry of pierced earrings & necklace. The lady's shapely original body is cotton with stitched joints at hips and knees so she sits very well. Her arms are kid with stitched fingers and separate thumbs, floppy-jointed at shoulders and elbows (small repair to left hand; lower right leg is an old replacement). Sweetly dressed in 2-piece red calico with white polka-dots and a lovely lace collar. Underneath are two eyelet petticoats, a chemise, and split drawers with tucks at the hems. Cordovan leather side-button boots and cotton knit socks complete her costume. She's an exquisite and special lady.
We ship UPS, Post Office or any shipping company that you request. We do require insurance. If you want your item shipped without insurance then you must write us and tell us you are shipping at your risk including out of the country shipping. All items shipped outside the US require a phone number. You can call our store and pay with all major credit cards, Visa, Master Card, Discover, American Express. You may also pay with a check. Our phone number is (303)722-8700 or (303)778-7077. Our business name is Turn of the Century Antiques our address is 1475 South Broadway Denver, Co 80210 we are open Tuesday Through Saturday 9:30 to 5:30 MST Thanks !
Item ID: 808
5 other shoppers have this item in their Cart or Wish List.
Dolls and toys are our passion at Turn of the Century Antiques.
We always offer a 30 day layaway! We are proud members of NADDA - the National Association of Doll Dealers.
Price for shipping to USA
change location
*UPS Ground


text:
By Official release
Posted May 22 2012 3:38PM
NEW YORK -- The Cleveland Cavaliers' Kyrie Irving, the 2011-12 Kia NBA Rookie of the Year, and the Minnesota Timberwolves' Ricky Rubio headline the 2011-12 NBA All-Rookie First Team, the NBA announced today. Irving was the lone unanimous selection.
Joining Irving (58 points) and Rubio (49 points) on the NBA All-Rookie First Team are Denver's Kenneth Faried (46 points), Golden State's Klay Thompson (43 points), New York's Iman Shumpert (40 points), San Antonio's Kawhi Leonard (40 points) and Detroit's Brandon Knight (40 points).
Irving, the league's top-scoring rookie, was named Eastern Conference Rookie of the Month for games played in December/January, February and March. He finished the season with averages of 18.5 points, 5.4 assists, 3.7 rebounds and 1.1 steals, becoming one of only six rookies in league history to average at least 18.0 points, 5.0 assists, and 1.0 steal while shooting at least .450 from the field (Alvan Adams, Grant Hill, Tyreke Evans, Magic Johnson, and Michael Jordan).
Rubio, the Western Conference Rookie of the Month for games played in December/January, appeared in 41 games during an injury-shortened debut season, averaging 10.6 points, a rookie-best 8.2 assists, and 4.2 rebounds. He also led rookies with 2.2 steals.
Faried averaged 10.2 points, a rookie-best 7.7 rebounds and 1.0 block for Denver, garnering Western Conference Player of the Month honors for games played in April. His .586 field goal percentage paced first-year players.
Thompson averaged 12.5 points while shooting a rookie-best .414 from three-point range, 2.4 rebounds and 2.0 assists.
Shumpert, Leonard and Knight tied for the fifth spot on the All-Rookie First Team. Shumpert averaged 9.5 points, 3.2 rebounds, 2.8 assists and 1.7 steals. Leonard posted 7.9 points, 5.1 rebounds, 1.3 steals and 1.1 assists. Knight tallied averages of 12.8 points, 3.8 assists and 3.2 rebounds.
The NBA All-Rookie Second Team consists of Houston's Chandler Parsons (33 points), Sacramento's Isaiah Thomas (27 points), New Jersey's MarShon Brooks (18 points), Minnesota's Derrick Williams (16 points) and Cleveland's Tristan Thompson (16 points).
The voting panel consisted of the NBA's 30 head coaches, who were asked to select five players for the first team and five players for the second team, regardless of position. Coaches were not permitted to vote for players on their own team. Two points were awarded for first team votes and one for second team votes.
Attached are the results of the balloting for the 2011-12 NBA All-Rookie teams. The balloting was tabulated by the independent accounting firm of Ernst & Young LLP.
* * *
* * *
Other players receiving votes, with point totals (first place votes in parentheses): Markieff Morris, Phoenix, 7 (1); Kemba Walker, Charlotte 7; Alec Burks, Utah 2; Norris Cole, Miami 2; Bismack Biyombo, Charlotte 2; Enes Kanter, Utah 1; Greg Stiemsma, Boston 1; Gustavo Ayon, New Orleans 1; Nikola Vucevic, Philadelphia 1.
|Hickson Block
J.J. Hickson with a big block during the second quarter.
|GameTime: Josh Smith Waived by Pistons
The crew discusses Josh Smith being waived by the Detroit Pistons.
|Walker to Biyombo
Bismack Biyombo takes the pass and finishes with authority.
|Mozgov Injury
Timofey Mozgov gets hit in the face during the first quarter.
|Fantasy Minute - Pickups of the Week
n need of some fantasy help? The Starters have a few suggestions which can give your team a boost.


text:
I bought these thinking I would wear them to the office on more casual days, but the culture is proving otherwise after one day of wear. I already have my everyday sneakers that I wear when I'm not at work, so these sadly have to go. They are in very good condition and come with extra laces in navy. Willing to part with them for $65+Shipping.
9/6/14 at 9:05am


text:
Kansas City shootings highlight threat of 'lone wolf' attacks
Frazier Glenn Miller was for years known as an active and avid white supremacist, but no one foresaw Sunday's shooting rampage in a Kansas City suburb. More ▸
Frazier Glenn Miller was for years known as an active and avid white supremacist, but no one foresaw Sunday's shooting rampage in a Kansas City suburb. More ▸
A New Jersey teenager was charged with multiple counts of bias intimidation after he allegedly tried to lure Orthodox women as part of a gang initiation. More ▸
Possibilities raised during efforts to resolve the current crisis over Israeli-Palestinian peace talks include freeing Jonathan Pollard, a partial settlement freeze, guarantees that Palestinians won't push for international recognition and a mass prisoner release. More ▸
The number of anti-Semitic incidents in the United States decreased by 19 percent in 2013, but physical assaults against Jews increased, according to the Anti-Defamation League. More ▸
Leaders of national Jewish groups resumed a dialogue with mainline Christian churches ruptured by a call in 2012 by some Christian leaders to consider cutting U.S. assistance to Israel. More ▸
Jewish leaders condemned Ayatollah Ali Khameini, Iran's supreme leader, for again questioning the Holocaust. More ▸
The Anti-Defamation League's national director was "saddened" by how President Obama welcomed Israeli Prime Minister Benjamin Netanyahu. More ▸
A new House resolution urges the State Department to renegotiate the terms for the return to Iraq of an archive of Iraqi Jewish texts. More ▸
Two national Jewish organizations are opposing a Maryland bill that would financially penalize colleges involved in an academic boycott of another country. More ▸
Israeli President Shimon Peres in a letter to Abraham Foxman expressed his "deep appreciation" to the Anti-Defamation League national director's "50-year service to the Jewish cause." More ▸
From our partners


text:
Anti-Inflammatory Action of Insulin via Induction of Gadd45-β Transcription by the mTOR Signaling Pathway.
ABSTRACT Insulin regulates a large number of genes in a tissue-specific manner. We have identified genes modulated by insulin in the liver and in liver-derived cells that had not yet been characterized as insulin regulated, and these previous studies indicate that numerous genes are induced by insulin via the MEK-ERK pathway. We now describe new studies indicating that Gadd45-β can be induced by acute insulin treatment. Although other regulators of Gadd45-β expression may utilize the MEK-ERK pathway, the data indicate that insulin utilizes signaling pathways separate from either MEK-ERK, PI3-K or p38 signaling pathways in the regulation of Gadd45-β transcription. Our findings show that activation of a downstream effector of multiple signaling pathways, mTOR, was required for insulin-induction of Gadd45-β gene transcription. Increased expression of Gadd45-β can inhibit c-Jun N-terminal kinase (JNK) activity. Since TNFα is increased during inflammation, and acts, at least in part, via the JNK signaling pathway, insulin induction of Gadd45-β suggests a mechanism for the anti-inflammatory actions of insulin.
- [Show abstract] [Hide abstract]
ABSTRACT: In recent years, the roles of chronic stress and depression as an independent risk factor for decreased insulin sensitivity and the development of diabetes have been increasingly recognized. However, an understanding and the mechanisms linking insulin resistance and acute psychological stress are very limited. We hypothesized that acute psychological stress may cause the development of insulin resistance, which may be a risk factor in developing type 2 diabetes. We tested the hypothesis in a well-established mouse model using 180 episodes of inescapable foot shock (IES), followed by a behavioral escape test. In this study, mice that received IES treatment were tested for acute insulin resistance by measuring glucose metabolism and insulin signaling. When compared to normal and sham mice, mice that were exposed to IES resulting in escape failure (defined as IES with behavioral escape failure) displayed elevated blood glucose levels in both glucose tolerance and insulin tolerance tests. Furthermore, mice with IES exposure and behavioral escape failure exhibited impaired hepatic insulin signaling via the insulin-induced insulin receptor / insulin receptor substrate 1 / Akt pathway, without affecting similar pathways in skeletal muscle, adipose tissue and brain. Additionally, a rise in murine growth-related oncogene KC/GRO was associated with impaired glucose metabolism in IES mice, suggesting a mechanism by which psychological stress by IES may influence glucose metabolism. The present results indicate that psychological stress induced by IES can acutely alter hepatic responsiveness to insulin and affect whole-body glucose metabolism.Journal of Endocrinology 02/2013; · 4.06 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The Gadd45 proteins have been intensively studied, in view of their important role in key cellular processes. Indeed, the Gadd45 proteins stand at the crossroad of the cell fates by controlling the balance between cell (DNA) repair, eliminating (apoptosis) or preventing the expansion of potentially dangerous cells (cell cycle arrest, cellular senescence), and maintaining the stem cell pool. However, the biogerontological aspects have not thus far received sufficient attention. Here we analyzed the pathways and modes of action by which Gadd45 members are involved in aging, longevity and age-related diseases. Because of their pleiotropic action, a decreased inducibility of Gadd45 members may have far-reaching consequences including genome instability, accumulation of DNA damage, and disorders in cellular homeostasis - all of which may eventually contribute to the aging process and age-related disorders (promotion of tumorigenesis, immune disorders, insulin resistance and reduced responsiveness to stress). Most recently, the dGadd45 gene has been identified as a longevity regulator in Drosophila. Although further wide-scale research is warranted, it is becoming increasingly clear that Gadd45s are highly relevant to aging, age-related diseases (ARDs) and to the control of life span, suggesting them as potential therapeutic targets in ARDs and pro-longevity interventions.Ageing research reviews 01/2012; 11(1):51-66. · 5.62 Impact Factor
Page 1
© 2010 Bortoff et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article
which permits unrestricted noncommercial use, provided the original work is properly cited.
Hepatic Medicine: Evidence and Research 2010:2 79–85
Hepatic Medicine: Evidence and ResearchDovepress
submit your manuscript | www.dovepress.com
open access to scientific and medical research
Open Access Full Text Article
Anti-inflammatory action of insulin via induction
of Gadd45-β transcription by the mTOR signaling
Katherine D Bortoff1
Adam B Keeton1
J Lee Franklin1
Joseph L Messina1,2
1Department of Pathology, Division
of Molecular and Cellular Pathology,
The University of Alabama at
Birmingham, Birmingham, Alabama,
USA; 2Veterans Affairs Medical Center,
Birmingham, Alabama, USA
Correspondence: Joseph L Messina
Department of Pathology, Division of
Molecular and Cellular Pathology, VH
G019, 1530 3rd Ave S., University of
Alabama at Birmingham, Birmingham,
Tel +1 (205) 934-4921
Fax +1 (205) 975-1126
Email email@example.com
Abstract: Insulin regulates a large number of genes in a tissue-specific manner. We have
previously identified genes modulated by insulin in the liver and in liver-derived cells that have
not yet been characterized as insulin regulated, and results of these previous studies indicated
that numerous genes are induced by insulin via the MEK-ERK pathway. We now describe new
studies indicating that Gadd45-β can be induced by acute insulin treatment. Although other
regulators of Gadd45-β expression may utilize the MEK-ERK pathway, the data indicate that
insulin utilizes signaling pathways separate from either MEK-ERK, PI3-K, or p38 signaling
pathways in the regulation of Gadd45-β transcription. Our findings show that activation of a
downstream effector of multiple signaling pathways, mTOR, was required for insulin-induction
of Gadd45-β gene transcription. Increased expression of Gadd45-β can inhibit c-Jun N-terminal
kinase (JNK) activity. Since TNFα is increased during inflammation, and acts, at least in part,
via the JNK signaling pathway, insulin induction of Gadd45-β suggests a mechanism for the
anti-inflammatory actions of insulin.
Keywords: insulin, Gadd45−β, mTOR, ERK, PI3-kinase, p38, JNK
Insulin regulates hepatic cellular processes involved in normal metabolism,
development, and growth, including enzyme activity, gene transcription, and DNA
and protein synthesis.1 In response to insulin binding, the insulin receptor becomes an
active tyrosine kinase resulting in tyrosine autophosphorylation of the insulin receptor
and phosphorylation of insulin receptor substrate (IRS) proteins, Son of Sevenless
(SOS), and the growth factor receptor binding protein [GRB-22]. This signaling cascade
leads to the activation of Ras and MEK kinase, which in turn activates the extracellular
signal-regulated kinases [ERK1/2;3].
A second major insulin-regulated signaling pathway is the phosphatidylinositol
3-OH-kinase (PI3-K) pathway, which is activated by recruitment of effector molecules
to the tyrosine phosphorylated IRS proteins. In some systems, insulin activates several
additional pathways, including those of p38 kinase and Cbl/CAP.2 Results of our pre-
vious work demonstrated that complex coordination of signaling pathways activated
by insulin, including interactions of insulin-activated MEK-ERK and p38 signaling
pathways, and the MEK-ERK and PI3-K pathways rapidly induced transcription of
the c-fos, Pip92 (also known as CHX-1, ETR101, IER2), Egr-1 (NGF-1A, Krox24,
zif268, and TIS8), ATF-3 (LRF-1, LRG-21, CRG-5, and TI-241), Insig-1 (CL-6), and
Krox20 (EGR-2) genes.4–6 We have also observed that insulin-induced gene expression
depends not only on activation of signaling pathways, but the temporal expression
patterns of these pathways.6
Number of times this article has been viewed
This article was published in the following Dove Press journal:
Hepatic Medicine: Evidence and Research
23 June 2010
Page 2
Hepatic Medicine: Evidence and Research 2010:2
submit your manuscript | www.dovepress.com
Bortoff et al
Another insulin activatable kinase, downstream of
multiple signaling pathways, is mammalian target of rapamy-
cin [mTOR;7–10]. This pathway is important in both insulin
and in nutrient signaling. The activity of mTOR is inhibited
by the fungal antibiotic rapamycin, which blocks the phos-
phorylation and activation of p70-S6 kinase and 4E-BP1,
which are substrates of mTOR.8,11–14
We have utilized the H4IIE rat hepatoma cells as a model
system.15,16 These cells express functional insulin and growth
hormone receptors, respond to physiologic concentrations of
both,17,18 and this response has been utilized in the study of
intracellular signaling and subsequent gene expression. We
previously identified several insulin-responsive genes through
differential screening of an insulin/anisomycin treated cDNA
library isolated from H4IIE cells.19 These include cytoskeletal
genes, and genes involved in cell division and the response
to cellular stress.4–6,20–24 Many of these genes are described
as immediate early genes (IEG) due to their rapid activation
in response to insulin.1,25
An additional cDNA that was recently selected was
identified to be homologous to Gadd45-β. The Gadd45
isoforms (α, β, γ) function in common, but also by distinct
pathways.26 They are induced by a number of cell stressors
and have a number of cellular functions, including cell cycle
regulation, from which they derive their names, growth
arrest and DNA damage-inducible factors.27 Their increased
abundance contributes to a low mitotic index and protection
of genomic integrity.26 Conversely, they are downregulated
in hepatocellular carcinoma.28 Gadd45-β is a major player
in NFκB mediated resistance to apoptosis and antagonizes
TNFα cytotoxicity by suppressing TNFα induced c-Jun
N-terminal kinase (JNK) activation. The Gadd45-β protein
forms a complex with MKK7, the upstream regulator of
JNK, inhibiting JNK activation.27,29,30
In addition to the many metabolic actions of insulin, it
plays the role of an anti-inflammatory agent. Insulin reduces
proinflammatory mediators such as TNFα, IL6, JE, and KC
in animal models of endotoxemia.31 Several proinflamma-
tory cytokines, in particular IL-6, signal via STAT3. We
and other researchers have found that an anti-inflammatory
action of insulin is to decrease STAT3 activity.32–34 Our recent
work indicates that the JNK pathway plays a role in TNFα
dependent injury-induced hepatic insulin resistance.35 TNFα
is increased during inflammation, and can lead to insulin
resistance, at least in part via activating JNK.
Regulation of Gadd45-β is complex and may involve
multiple signaling pathways. To our knowledge, the present
study is the first report demonstrating active insulin-induced
transcription of Gadd45-β. Inhibition of the MEK-ERK and
PI3-K pathways were ineffective in altering the insulin effect
on Gadd45-β transcription. However, mTOR was found to be
necessary, since inhibition of this kinase abolished insulin-
induced Gadd45-β transcription. Since insulin increased
expression of Gadd45-β, which can inhibit JNK activity,
insulin induction of Gadd45-β suggests a mechanism for
the anti-inflammatory actions of insulin.
Materials and methods
Swim's 77 medium, fetal bovine serum, calf serum, horse
serum, and wortmannin were obtained from Sigma-Aldrich,
(St Louis, MO). RNase H, and proteinase K were purchased
from Gibco/BRL (Bethesda, MD). RNAsin was obtained
from Promega (Madison, WI), DNase I and RNase T1 from
Worthington/Cappel Biochemicals, Inc. (Lakewood, NJ).
LY294002 was purchased from Biomol (Plymouth Meeting,
PA), PD98059 was obtained from Cell Signaling Technol-
ogy (Beverly, MA) as were phospho-(active)-ERK1/2,
phospho-(Ser473)-Akt, and secondary rabbit antisera, and
International Chemical and Nuclear Corp. (ICN, Costa Mesa,
CA) supplied the α-32P-UTP.
Cell culture
Rat H4-IIE hepatoma cells (American Type Culture Collec-
tion, Rockville, MD) were maintained in monolayer cultures
in Swim's 77 medium supplemented with 2% fetal bovine
serum, 3% calf serum, and 5% horse serum in a 5% CO2
incubator.4,17,19 Experiments were initiated after serum was
withdrawn for 20 to 24 hours when cells were approximately
70% to 80% confluent.
Transcription assays
Transcription rates of the mRNAs of interest were measured
in isolated nuclei by the nuclear run-on method exactly as
described previously.5,17,19 Newly transcribed nuclei were
labeled with 32P-UTP. The nascent transcripts were isolated,
hybridized with cDNAs dot-blotted onto nitrocellulose, then
exposed to autoradiography. The resultant autoradiograms
were densitometrically scanned and analyzed using ZeroD
Scan from Scanalytics (Fairfax, VA). The values from the
treatments were compared to the control in each experiment
and expressed as fold-changes.
Western blot analysis
Whole cell lysates were resolved by SDS-PAGE and trans-
ferred to Protran BA85 membranes (Schleicher and Schuell,
Page 3
Hepatic Medicine: Evidence and Research 2010:2
Figure 1 Induction of Gadd45-β transcription by insulin is independent of MEK
activity. Serum-deprived H4IIE cells were treated with 10 nM insulin alone for 30
minutes (I) or in the presence of the MEK inhibitor PD98059 (PD; 50 µM) added
30 minutes prior to the insulin. Transcription was measured by nuclear run-on assay
as described in Material and methods. (A and B). Levels of ERK1/2 phosphorylation
(C) were determined by Western blot of whole cell lysates using phospho-specific
antisera. Representative autoradiograms are shown (A and C) along with mean data
and standard error (SEM) collected from three or more experiments at each time
point (B). Statistical significance: # = P , 0.05 and ## = P , 0.01 versus vehicle
Insulin (min)
β tubulin
Fold change in transcription
Insulin (min)
05 15120
Insulin (min)
05515 120
0 30120
submit your manuscript | www.dovepress.com
Insulin regulation of Gadd45-β
Keene, NH), developed with ECL Plus (Amersham Biosci-
ences, Buckinghamshire, England), and visualized by autora-
diography or direct digital imaging of chemiluminescent blots
using the Fluorchem FC imager system [Alpha Innotech, San
Leandro, CA;4,18,36]. Quantitation of the relative band inten-
sity and background subtraction was carried out using the
onboard software of the Fluorchem FC digital imager.4,37,38
Statistical analysis
ANOVA and Student's t tests were performed using the
Instat program (Graphpad Software Inc., San Diego, CA).
Values from experimental treatments were expressed as fold
changes compared to the vehicle-treated controls within each
experiment. #, ##, and ###, designate P , 0.05, P , 0.01,
and P , 0.001, respectively, versus vehicle control.
A cDNA library was constructed from H4IIE cells treated
with a postprandial concentration of insulin (1 × 10−8 M)
and anisomycin (100 µM) for 2 hours. Radiolabeled cDNAs
isolated from similarly treated cells were then compared with
labeled cDNAs from untreated cells in the screening of this
library.19 Gadd45-β was selected as a differentially expressed
mRNA. When independently tested using the nuclear run-on
assay, a consistent and significant 2- to 3-fold increase of
Gadd45-β transcription was induced by addition of insulin
for 30 minutes (P , 0.001; Figures 1A and B).
It was then asked which insulin-induced signaling
pathway(s) was necessary for insulin to increase Gadd45-β
gene expression. In our prior reports it was demonstrated
that the MEK-ERK pathway is required for the rapid
insulin induction of c-fos, Pip92, Egr-1, ATF-3, Insig-1,
and Krox20 transcription.4–6 A specific inhibitor of MEK,
PD98059, was used in these prior studies and found to abolish
insulin-induced transcription of these genes. When PD98059
was used to determine the requirement for the MEK-ERK
pathway in insulin induction of Gadd45-β transcription, the
inhibitor itself had no effect on basal Gadd45-β transcription.
Unlike our earlier observations regarding insulin regulation
of c-fos, Pip92, Egr-1, ATF-3, Insig-1, and Krox20 tran-
scription, insulin-induced transcription of Gadd45-β was
not significantly attenuated by pretreatment with PD98059
(Figure1B). This slight but insignificant effect on insulin-
induced Gadd45-β transcription was observed at time points
where insulin-stimulated phosphorylation of ERK1/2 was
completely blocked. For instance, insulin for 5 minutes
resulted in a maximal activation/phosphorylation of ERK1/2
(Figure 1C, top panel). The phosphorylation of ERK1/2
decreased by about half by 15 minutes and was still measur-
ably increased following 120 minutes of continuous insulin
treatment. Pretreatment of cells with PD98059 completely
abolished insulin-activation of ERK1/2 to levels similar to
Page 4
Hepatic Medicine: Evidence and Research 2010:2
Figure 2 Induction of Gadd45-β transcription by insulin is independent of PI3-K
activity. Serum-deprived H4IIE cells were treated with 10 nM insulin alone for 30
minutes or in the presence of the PI3-K inhibitor, LY294002 (at 50 or 100 µM,
as indicated), added 30 minutes prior to the insulin. Whole cell lysates were
prepared and Western analysis was performed. A representative autoradiogram
demonstrating levels of Akt Ser-473 phosphorylation is shown (A). Transcription
rates were measured by nuclear run on assays and (B) the mean data was collected
from three or more experiments at each time point. Statistical significance: ## =
P , 0.01 versus vehicle control.
Insulin (min)
Insulin (min)
LY294002 (µM)
Fold change in transcription
submit your manuscript | www.dovepress.com
Bortoff et al
eliminated the additive/synergistic effect of SB202190 and
insulin on Gadd45-β transcription, decreasing it to the level
of the insulin effect alone (Figure 3). Thus, the SB202190
effect was inhibited by PD98089 addition, and the MEK-
ERK signaling pathway was utilized, as we have recently
described in the study of other insulin regulated genes.4,5
In contrast, the insulin effect was not blocked by PD98059
when cells were treated with insulin alone or in combination
with SB202190 (Figures 1B and 3), again suggesting that
insulin uses multiple pathways, or a separate pathway.
To determine if insulin-regulation of Gadd45-β transcrip-
tion occurred via insulin activation of mTOR, cells were
pretreated with rapamycin for 60 minutes followed by insulin
stimulation for 30 minutes. Rapamycin itself had no effect
on basal Gadd45-β gene transcription (Figure 4); however,
rapamycin completely inhibited the insulin induction of
Gadd45-β, decreasing transcription to basal levels. This
indicates that the mTOR pathway is important for insulin's
regulation of Gadd45-β gene expression.
levels observed in vehicle controls at all time points tested
(Figure 1C, bottom panel). Since phosphorylation of ERK1/2
was completely blocked by the MEK 1 inhibitor, yet insulin
induction of Gadd45-β transcription was not significantly
altered, this indicates that the MEK-ERK pathway alone is
not a primary signaling pathway used by insulin to regulate
Gadd45-β transcription.
A second major signaling pathway activated by insu-
lin, the PI3-K pathway, was then investigated to discover
whether insulin regulated transcription of Gadd45-β was
dependent on this signaling pathway. Insulin treatment
rapidly increased signaling via PI3-K as shown by a rapid
increase in phosphorylation/activation of the PI3-K effector
Akt (Figure 2A). This increase in PI3-K activity by insulin
was blocked by pretreatment with a competitive inhibitor of
PI3-K, LY294002. However, there was no alteration in either
basal or the insulin induction of Gadd45-β transcription in the
presence of two concentrations of this inhibitor (Figure 2B).
A second, less specific inhibitor of PI3-K, wortmannin, which
binds to the catalytic p110 subunit of PI3-K, was also used.
Similar to the action of LY294002, wortmannin alone did
not affect basal transcription levels of Gadd45-β, nor did it
significantly alter insulin-induced Gadd45-β transcription
(data not shown). Thus, it is unlikely that the PI3-K pathway
alone, and its downstream kinase signaling cascade, is a pri-
mary signaling pathway used by directly insulin in regulation
of Gadd45-β transcription.
We previously demonstrated that the p38 pathway, a par-
allel MAPK signaling pathway regulated by insulin, may
negatively regulate ERK1/2 signaling and gene transcription
in H4IIE cells.4,5 A specific inhibitor of p38, SB202190,
was used to assess whether inhibition of p38, (which would
in turn increase ERK1/2 activity) would affect insulin-
induction of Gadd45-β transcription. However, pretreatment
of H4IIE cells with SB202190 alone significantly induced
Gadd45-β transcription (P , 0.01), to a level at least equal
to that observed with insulin alone (Figure 3). Pretreatment
with SB202190 followed by insulin stimulation resulted in
an additive or possibly synergistic induction (7.4-fold) of
Gadd45-β compared to the induction by either insulin or
SB202190 treatment alone (2.4- or 3.5-fold, respectively).
This suggests that the p38 pathway is a negative regulator
of Gadd45-β transcription.
It was then asked whether the additive induction of
Gadd45-β gene transcription by insulin and SB202190
required the activation of the MEK-ERK pathway. For these
studies, H4IIE cells were pretreated with PD98059 prior
to the addition of SB202190 and insulin. This treatment
Page 5
Hepatic Medicine: Evidence and Research 2010:2
Figure 4 Inhibition of insulin-regulated Gadd45-β transcription by rapamycin.
Serum-deprived H4IIE cells were treated with 10 nM insulin alone for 30 minutes
or in the presence of the mTOR inhibitor, rapamycin (10 µM), added 30 minutes
prior to the insulin. Nuclear run-on assays were then performed. The mean data was
collected from three or more experiments at each time point. Statistical significance:
### = P , 0.001 versus vehicle control.
Insulin (min)
Rapamycin (min)
Fold change in transcription
submit your manuscript | www.dovepress.com
Insulin regulation of Gadd45-β
We then examined which signaling pathway(s) are used by
insulin in the regulation of Gadd45-β transcription. We have
previously reported on the involvement of the MEK-ERK and
p38 signaling pathways in insulin-regulated gene expression.
For instance, the rapid induction of ERK was absolutely nec-
essary for insulin-induction of c-fos, Pip92, Egr-1, ATF-3,
Insig-1, and Krox20 transcription.4,5,39 We therefore expected
that this would be the case for insulin induction of Gadd45-β.
However, unlike these other genes, blocking insulin-induced
activation of MEK-ERK had little effect on insulin-regulated
transcription of Gadd45-β, suggesting that the MEK-ERK
signaling pathway was not a major pathway in insulin regula-
tion of Gadd45-β transcription.
This finding, that insulin does not use the MEK-ERK
pathway, contrasts with the published accounts on the
involvement of MEK-ERK in Gadd45 gene expression. For
instance, MEK-ERK is necessary for the full induction of
the Gadd45 promoter following UV-induced DNA damage in
both HCTII6 human colorectal carcinoma cells and MCF-7
human breast cancer cells.37 Also, inhibition of ERK activity
decreases Gadd45 expression in the ovarian carcinoma cell
line A2780 in response to cisplatin-induced DNA damage.41
The present data indicate that insulin does not regulate
Gadd45 expression via the MEK-ERK pathway; rather, the
data suggest that the p38 pathway is a negative inhibitor of
Gadd45-β transcription, and when the p38 pathway is inhib-
ited, both MEK-ERK signaling and Gadd45-β expression
are increased. This is similar to our previous observations
that inhibition of p38 activity results in activation of the
MEK-ERK pathway.4,5 However, when the MEK1 (MEK-
ERK) inhibitor was added prior to addition of SB202190
and insulin, transcription of Gadd45-β was reduced only
to the levels observed with insulin treatment alone. These
data demonstrate that activation of the MEK-ERK pathway
can result in induction of Gadd45-β transcription in the rat
H4IIE hepatoma cell line, and that even though insulin is a
strong activator of the MEK-ERK pathway, this pathway is
not utilized by insulin in the regulation of Gadd45-β tran-
scription in H4IIE cells.
Clearly, inhibition of PI3-K signaling also did not alter
basal or insulin-induced regulation of the Gadd45-β gene.
An additional component of insulin signaling is the mTOR
kinase, which is important in controlling protein synthesis and
cell proliferation in response to changes in nutrients.12,14,42–45
The present studies demonstrated that treatment of H4 cells
with rapamycin, an inhibitor of mTOR activity, completely
blocked insulin's induction of Gadd45-β gene transcription.
Since blocking an upstream activator of mTOR, PI3-K,
While searching for insulin-induced genes, we selected a
cDNA that was inducible by insulin, and, when sequenced,
this cDNA encoded Gadd45-β, one of a family of growth
arrest and DNA damage-inducible genes. To our knowledge,
this is the first report of an insulin effect on expression of
the Gadd45-β gene.
Insulin (min)
SB202190 (µΜ)
PD98059 (µΜ)
Fold change in transcription
Figure 3 Effect of MEK1 and p38 inhibition on induction of Gadd45-β transcription
by insulin. H4IIE cells were serum-deprived and pretreated with PD98059 (50 µM)
prior to the addition of SB202190 (10 µM) with or without insulin (10 nm) for
30 minutes. Transcription was then measured by nuclear run-on assays. The mean
data was collected from three or more experiments at each time point. Statistical
significance: #, ##, and ###, designate P < 0.05, P < 0.01, and P < 0.001, respectively
versus vehicle control. *** = P < 0.001 versus insulin treated and SB202190 treated
Page 6
Hepatic Medicine: Evidence and Research 2010:2
submit your manuscript | www.dovepress.com
Bortoff et al
had no effect on insulin regulated Gadd45-β transcription,
it suggests that activation of PI3-K is not the main or only
signaling pathway used by insulin to activate mTOR in these
cells. While PI3-K has been found to be a common activator
of mTOR in many cells, in skeletal muscle passive stretch
induces mTOR independent of the PI3-K pathway46 and
other reports have implicated phospholipase D (PLD) in
mTOR signaling.47,48 Most evidence suggests that the MEK-
ERK pathway is separate from, but may impinge upon, the
mTOR signaling pathway downstream of the mTOR complex
In summary, our findings suggest that neither the MEK-
ERK nor PI3-K signaling pathways are predominantly used in
insulin regulation of Gadd45-β. Thus, we propose that some
other insulin-responsive pathway is necessary to activate
mTOR and insulin-dependent mTOR-regulated Gadd45-β
transcription, suggesting a novel mechanism for insulin
regulation of Gadd45-β transcription. An alternative explana-
tion is that insulin can activate mTOR by activating multiple
signaling pathways which converge on mTOR. In this case,
inhibition of either the MEK-ERK or the PI3-K pathways
would be insufficient to completely reduce insulin-induced
mTOR and therefore mTOR-dependent gene expression.
Unfortunately, inhibition of both of these pathways simulta-
neously is not possible due to the toxicity of this combination
treatment to the cell line used. This study, in combination with
our previous studies on insulin-regulated gene expression,
demonstrates the complexity of signaling pathways involved
in insulin regulation of gene expression in general, and in the
regulation of Gadd45-β expression in particular.
Increased Gadd45-β expression antagonizes TNFα cyto-
toxicity by suppressing TNFα-induced JNK activation.27,29,30
Increased TNFα and activation of the JNK signaling path-
way are known to contribute to chronic states of insulin
resistance.53–57 Our recent work indicates that the JNK path-
way also plays a role in TNFα dependent injury-induced
acute hepatic insulin resistance.32 By increasing expression
of Gadd45-β, insulin may be able to decrease JNK activity,
decreasing the inflammatory response and insulin resistance.
Thus, we hypothesize that insulin induction of Gadd45-β gene
expression is a novel mechanism for the anti- inflammatory
effects of insulin.
The research was supported by grants from the National
Institutes of Health (DK 62071), the Department of Defense
(W81XWH-0510387), and the Veterans Administration Merit
Review to Dr Messina.
The authors report no conflicts of interest in this work.
1. Messina JL. Insulin as a growth-promoting hormone. In: JL Kostyo,
editor. Handbook of physiology, section 7: the endocrine system.
Volume V: hormonal control of growth. New York: Oxford University
Press; 1999. p. 783–811.
2. Virkamaki A, Ueki K, Kahn CR. Protein-protein interaction in insulin
signaling and the molecular mechanisms of insulin resistance. J Clin
Invest. 1999 April;103:931–943.
3. Whitmarsh AJ, Davis RJ. Structural organization of MAP-kinase signal-
ing modules by scaffold proteins in yeast and mammals. [Review] [33
refs]. Trends in Biochemical Sciences. 1998;23:481–485.
4. Keeton AB, Amsler MO, Venable DY, Messina JL. Insulin Signal
Transduction Pathways and Insulin-induced Gene Expression. J Biol
Chem. 2002;277:48565–48573.
5. Keeton AB, Bortoff KD, Bennett WL, et al. Insulin-regulated expres-
sion of Egr-1 and Krox20: dependence on ERK1/2 and interaction
with p38 and PI3-kinase pathways. Endocrinology. 2003;144:
6. Keeton AB, Bortoff KD, Franklin JL, Messina JL. Blockade of rapid
versus prolonged extracellularly regulated kinase 1/2 activation has
differential effects on insulin-induced gene expression. Endocrinology.
7. Fernandez AM, Kim JK, Yakar S, et al. Functional inactivation of the
IGF-I and insulin receptors in skeletal muscle causes type 2 diabetes.
Genes Dev. 2001 August 1;15(15):1926–34.
8. Thomas G, Hall MN. TOR signalling and control of cell growth. Curr
Opin Cell Biol. 1997;9:782–787.
9. Li J, DeFea K, Roth RA. Modulation of insulin receptor substrate-1
tyrosine phosphorylation by an Akt/phosphatidylinositol 3-kinase
pathway. J Biol Chem. 1999;274:9351–9356.
10. Pessin JE, Saltiel AR. Signaling pathways in insulin action: molecular
targets of insulin resistance. J Clin Invest. 2000;106:165–169.
11. Shah OJ, Anthony JC, Kimball SR, Jefferson LS. 4E-BP1 and S6K1:
translational integration sites for nutritional and hormonal information
in muscle. Am J Physiol Endocrinol Metab. 2000;279:E715–E729.
12. Gingras AC, Raught B, Sonenberg N. Regulation of translation initiation
by FRAP/mTOR. Genes Dev. 2001;15:807–826.
13. Gingras AC, Raught B, Sonenberg N. Control of translation by the target
of rapamycin proteins. Prog Mol Subcell Biol. 2001;27:174.
14. Proud CG. mTOR-mediated regulation of translation factors by amino
acids. Biochem Biophys Res Commun. 2004;313:429–436.
15. Messina JL, Hamlin J, Larner J. Effects of insulin alone on the accu-
mulation of a specific mRNA in rat hepatoma cells. J Biol Chem.
16. Xu J, Keeton AB, Franklin JL, et al. Insulin enhances growth hor-
mone induction of the MEK/ERK signaling pathway. J Biol Chem.
17. Messina JL. Insulin and dexamethasone regulation of a rat hepatoma
messenger ribonucleic acid: insulin has a transcriptional and a post-
transcriptional effect. Endocrinology. 1989;124:754–761.
18. Ji S, Guan R, Frank SJ, Messina JL. Insulin inhibits growth hormone
signaling via the growth hormone receptor/JAK2/STAT5B pathway.
J Biol Chem. 1999;274:13434–13442.
19. Bortoff KD, Zhu CC, Hrywna Y, Messina JL. Insulin induction of
pip92, CL-6 and novel mRNAs in rat hepatoma cells. Endocrine.
20. Messina JL. Induction of cytoskeletal gene expression by insulin. Mol
Endocrinol. 1992;6:112–119.
21. Tollet P, Legraverend C, Gustafsson J-Å, Mode A. A role for protein
kinases in the growth hormone regulation of cytochrome P4502C12
and insulin-like growth factor-I messenger RNA expression in primary
adult rat hepatocytes. Mol Endocrinol. 1991;5:1351–1358.
Page 7
Hepatic Medicine: Evidence and Research
Publish your work in this journal
Hepatic Medicine: Evidence and Research is an international, peer-
reviewed, open access journal covering all aspects of adult and pedi-
atric hepatology in the clinic and laboratory including the following
topics: Pathology, pathophysiology of hepatic disease; Investigation
and treatment of hepatic disease; Pharmacology of drugs used for
Submit your manuscript here: http://www.dovepress.com/hepatic-medicine-evidence-and-research-journal
the treatment of hepatic disease. Issues of patient safety and quality
of care will also be considered. The manuscript management system
is completely online and includes a very quick and fair peer-review
system, which is all easy to use. Visit http://www.dovepress.com/
testimonials.php to read real quotes from published authors.
Hepatic Medicine: Evidence and Research 2010:2
submit your manuscript | www.dovepress.com
Insulin regulation of Gadd45-β
22. Messina JL, Standaert ML, Ishizuka T, Weinstock RS, Farese RV. Role
of protein kinase C in insulin's regulation of c-fos transcription. J Biol
Chem. 1992;267:9223–9228.
23. Messina JL, Weinstock RS. Evidence for diverse roles of protein
kinase-c in the inhibition of gene expression by insulin: the tyrosine
aminotransferase, albumin, and phosphoenolpyruvate carboxykinase
genes. Endocrinology. 1994;135:2327–2334.
24. Messina JL. Regulation of the gamma actin gene by insulin. J Cell
Physiol. 1994;160:287–294.
25. Messina JL. Regulation of gene expression by insulin. In: P Cuatrecasas,
SJ Jacobs, editors. Handbook of experimental pharmacology, Vol. 92:
insulin. NY: Springer-Verlag; 1990. p. 399–419.
26. Mak SK, Kultz D. Gadd45 proteins induce G2/M arrest and modulate
apoptosis in kidney cells exposed to hyperosmotic stress. J Biol Chem.
27. Tornatore L, Marasco D, Dathan N, et al. Gadd45 beta forms a
homodimeric complex that binds tightly to MKK7. J Mol Biol. 2008;
28. Qiu W, Zhou B, Zou H, et al. Hypermethylation of growth arrest DNA
damage-inducible gene 45 beta promoter in human hepatocellular
carcinoma. Am J Pathol. 2004;165:1689–1699.
29. Papa S, Monti SM, Vitale RM, et al. Insights into the structural basis
of the GADD45beta-mediated inactivation of the JNK kinase, MKK7/
JNKK2. J Biol Chem. 2007;282:19029–19041.
30. Papa S, Zazzeroni F, Bubici C, et al. Gadd45 beta mediates the NF-
kappa B suppression of JNK signalling by targeting MKK7/JNKK2.
Nat Cell Biol. 2004;6:146–153.
31. Kidd LB, Schabbauer GA, Luyendyk JP, et al. Insulin activation of the
phosphatidylinositol 3-kinase/protein kinase B (Akt) pathway reduces
lipopolysaccharide-induced inflammation in mice. J Pharmacol Exp
Ther. 2008;326:348–353.
32. Campos SP, Baumann H. Insulin is a prominent modulator of the
cytokine-stimulated expression of acute-phase plasma protein genes.
Mol Cell Biol. 1992;12:1789–1797.
33. Campos SP, Wang Y, Baumann H. Insulin modulates STAT3 pro-
tein activation and gene transcription in hepatic cells. J Biol Chem.
34. Xu J, Ji S, Venable DY, Franklin JL, Messina JL. Prolonged insulin
treatment inhibits GH signaling via STAT3 and STAT1. J Endocrinol.
35. Xu J, Kim HT, Ma Y, et al. Trauma and Hemorrhage-Induced Acute
Hepatic Insulin Resistance: Dominant Role of Tumor Necrosis Factor
(TNF)-alpha. Endocrinology. 2008;149:2369–2382.
36. Reks SE, Smith PH, Messina JL, Weinstock RS. Activation of PKC delta
by insulin in a rat hepatoma cell line. Endocrine. 1998;8:161–167.
37. Nelson JWed. Alpha Innotech hands on application note #26. 1-1-1999.
San Leandro, CA, Alpha Innotech Corporation. Ref Type: Pamphlet
38. Kendrick NC, Johansen JJ, Lee PR, Santek DA. Optimization of an HP
Scanjet for quantification of protein electrophoresis gels. Anal Biochem.
39. Keeton AB, Messina JL. Modulation of Elk-dependent-transcription
by Gene33. J Cell Biochem. 2005;94:1190–1198.
40. Tong T, Fan W, Zhao H, et al. Involvement of the MAP kinase path-
ways in induction of GADD45 following UV radiation. Exp Cell Res.
41. DeHaan RD, Yazlovitskaya EM, Persons DL. Regulation of p53 target
gene expression by cisplatin-induced extracellular signal-regulated
kinase. Cancer Chemother Pharmacol. 2001;48:383–388.
42. Oldham S, Hafen E. Insulin/IGF and target of rapamycin signaling: a
TOR de force in growth control. Trends Cell Biol. 2003;13:79–85.
43. Schmelzle T, Hall MN. TOR, a central controller of cell growth. Cell.
44. Fafournoux P, Bruhat A, Jousse C. Amino acid regulation of gene
expression. Biochem J. 2000;351:1–12.
45. van Sluijters DA, Dubbelhuis PF, Blommaart EF, Meijer AJ. Amino-
acid-dependent signal transduction. Biochem J. 2000;351:550.
46. Hornberger TA, Stuppard R, Conley KE, et al. Mechanical stimuli
regulate rapamycin-sensitive signaling by a phosphoinositide 3-kinase,
protein kinase B and growth factor independent mechanism. Biochem J.
47. Fang Y, Vilella-Bach M, Bachmann R, Flanigan A, Chen J. Phospha-
tidic acid-mediated mitogenic activation of mTOR signaling. Science.
48. Kam Y, Exton JH. Role of phospholipase D1 in the regulation of mTOR
activity by lysophosphatidic acid. FASEB J. 2004;18:311–319.
49. Lornejad-Schafer MR, Schafer C, Graf D, Haussinger D, Schliess F.
Osmotic regulation of insulin-induced mitogen-activated protein
kinase phosphatase (MKP-1) expression in H4IIE rat hepatoma cells.
Biochem J. 2003;371:609–619.
50. Von Manteuffel SR, Gingras AC, Ming XF, Sonenberg N, Thomas G.
4E-BP1 phosphorylation is mediated by the FRAP-p70s6k pathway and
is independent of mitogen-activated protein kinase. Proc Natl Acad Sci
51. Von Manteuffel SR, Dennis PB, Pullen N, Gingras AC, Sonenberg N,
Thomas G. The insulin-induced signalling pathway leading to S6 and
initiation factor 4E binding protein 1 phosphorylation bifurcates at a
rapamycin-sensitive point immediately upstream of p70s6k. Mol Cell
Biol. 1997;17:5426–5436.
52. Takuwa N, Fukui Y, Takuwa Y. Cyclin D1 expression mediated by
phosphatidylinositol 3-kinase through mTOR-p70(S6K)-independent
signaling in growth factor-stimulated NIH 3T3 fibroblasts. Molecular
and Cellular Biology. 1999;19:1346–1358.
53. Hirosumi J, Tuncman G, Chang L, et al. A central role for JNK in obesity
and insulin resistance. Nature. 2002;420:333–336.
54. Bennett BL, Satoh Y, Lewis AJ. JNK: a new therapeutic target for
diabetes. Curr Opin Pharmacol. 2003;3:420–425.
55. Nakatani Y, Kaneto H, Kawamori D, et al. Modulation of the JNK
pathway in liver affects insulin resistance status. J Biol Chem.
56. Ogston D, McAndrew GM. Fibrinolysis in obesity. Lancet. 1964;
57. Tuncman G, Hirosumi J, Solinas G, Chang L, Karin M,
Hotamisligil GS. Functional in vivo interactions between JNK1 and
JNK2 isoforms in obesity and insulin resistance. Proc Natl Acad Sci


text:
BluePhoenix Announces Executive Management Changes
BluePhoenix Solutions (Nasdaq: BPHX), the leader in value-driven legacy IT modernization solutions, today announced that Arik Kilman was appointed Chief Executive Officer replacing Yaron Tchwella who is leaving the position of Chief Executive Officer for personal reasons. Mr. Tchwella was nominated by the Board of Directors to serve as a Director. If approved by the Company's shareholders at the shareholders meeting which is expected to be held prior to the end of June 2010, Yaron will join the board and will serve as an executive focused on growing the Company's partner network and on strategic customers. Gur Shomron, a member of the Board of Directors was appointed to replace Mr. Kilman as Chairman of the Board.
"I return to lead BluePhoenix as CEO at a time when I am increasingly excited about our future," said Mr. Kilman. "The prospects for our solutions continue to grow and we strongly believe that now is the right time for legacy modernization. The market opportunity ahead of us is growing, we own an ever-improving business proposition for our customers and our reputation within the industry is unmatched."
About BluePhoenix Solutions
BluePhoenix Solutions (NASDAQ: BPHX) is the leading provider of value-driven legacy IT modernization solutions. The BluePhoenix portfolio includes a comprehensive suite of tools and services from global IT asset assessment and impact analysis to automated database and application migration, rehosting, and renewal. Leveraging over 20 years of best-practice domain expertise, BluePhoenix works closely with its customers to ascertain which assets should be migrated, redeveloped, or wrapped for reuse as services or business processes, to protect and increase the value of their business applications and legacy systems with minimized risk and downtime.BluePhoenix provides modernization solutions to companies from diverse industries and vertical markets such as automotive, banking and financial services, insurance, manufacturing, and retail. Among its prestigious customers are: Aflac, Capita Group, CareFirst, Citigroup, Danish Commerce and Companies Agency, Desjardins, Los Angeles County Employees Retirement Association, Rabobank, Rural Servicios Informaticos, SDC Udvikling, TEMENOS, Toyota, and Volvofinans. BluePhoenix has 13 offices in the USA, UK, Denmark, Germany, Italy, The Netherlands, Romania, Russia, Cyprus, Australia, and Israel.
Select the service that is right for you!COMPARE ALL SERVICES
Action Alerts PLUSTry it NOW
Jim Cramer and Stephanie Link actively manage a real portfolio and reveal their money management tactics while giving advanced notice before every trade.
Product Features:
- $2.5+ million portfolio
- Large-cap and dividend focus
- Intraday trade alerts from Cramer
- Weekly roundups
TheStreet Quant RatingsTry it NOW
Only $49.95/yr
Access the tool that DOMINATES the Russell 2000 and the S&P 500.
Product Features:
- Buy, hold, or sell recommendations for over 4,300 stocks
- Unlimited research reports on your favorite stocks
- A custom stock screener
- Upgrade/downgrade alerts
Stocks Under $10Try it NOW
David Peltier, uncovers low dollar stocks with extraordinary upside potential that are flying under Wall Street's radar.
Product Features:
- Model portfolio
- Stocks trading below $10
- Intraday trade alerts
- Weekly roundups
Dividend Stock AdvisorTry it NOW
Jim Cramer's protege, David Peltier, identifies the best of breed dividend stocks that will pay a reliable AND significant income stream.
Product Features:
- Diversified model portfolio of dividend stocks
- Alerts when market news affect the portfolio
- Bi-weekly updates with exact steps to take - BUY, HOLD, SELL
Real Money ProTry it NOW
All of Real Money, plus 15 more of Wall Street's sharpest minds delivering actionable trading ideas, a comprehensive look at the market, and fundamental and technical analysis.
Product Features:
- Real Money + Doug Kass Plus 15 more Wall Street Pros
- Intraday commentary & news
- Ultra-actionable trading ideas
Options ProfitsTry it NOW
Our options trading pros provide daily market commentary and over 100 monthly option trading ideas and strategies to help you become a well-seasoned trader.
Product Features:
- 100+ monthly options trading ideas
- Actionable options commentary & news
- Real-time trading community
- Options TV


text:
Program Schedule
What's Playing Now
Culture Connection
About Us
About Us
Meet the Staff
Photo Album
Ministry Connections
Frequencies/Coverage Area
Listener Stories
Contact Us
WBCL Apps
Our Community
Our Community
WBCL Text Alerts
Prayer Works
Opportunities to Serve
Business Partners
Facebook Fridays
Mid-Morning Fiction Fix
Support WBCL
Support WBCL
Business Partners
Giving Opportunities
Make A Gift
Memorial Gifts
Estate Planning
Pledge Payment Options
Day Sponsor
Write a Will Week
Share Volunteers
Outreach Ministries
Outreach Ministries
WBCL Let's Build a Home Project
Drive Thru Difference
Previous Outreach Ministries
Opportunities to Serve
Food Pantry
Bead For Life Christmas Shopping
WBCL VIP Volunteer
Disaster Relief
Contact Us
Jesu Bambino
Wayne Watson
view entire playlist
7:48 pm
Andy Williams
Happy Holiday
7:44 pm
Francesca Battistelli
Heaven Everywhere
7:39 pm
Casting Crowns
I Heard the Bells on Christmas Day
7:27 pm
Smalltown Poets
Good Christian Men Rejoice
Listen To Us
Mid-Morning Last Month's Archives
Search through our archives of Mid-Morning programs below. If the audio archive is not available to Listen Online or Download,
we probably still have the program audio in our o
ffices. You can order a CD from us.
To listen to the Mid-Morning shows please download the
and click on Podcats.
Take Note!
*If you are trying to search for something in the archives, you may notice searching is currently limited. We have to make some manual adjustments to each archived show to allow it to show up in the search, so in the meantime,
click "search" without typing anything in the box first...and this will bring up the full listing of shows. THEN you can type in the key word and find the program you're looking for!
Our apologies for the extra step...it's just temporary!
This Week
Monthly Archive
Full Archive
select a month
or search by keyword
December, 2014
November, 2014
October, 2014
September, 2014
August, 2014
July, 2014
June, 2014
May, 2014
April, 2014
March, 2014
February, 2014
January, 2014
December, 2013
November, 2013
October, 2013
September, 2013
August, 2013
July, 2013
June, 2013
May, 2013
April, 2013
March, 2013
February, 2013
January, 2013
December, 2012
November, 2012
October, 2012
September, 2012
August, 2012
July, 2012
June, 2012
May, 2012
April, 2012
March, 2012
February, 2012
January, 2012
December, 2011
November, 2011
October, 2011
September, 2011
August, 2011
July, 2011
June, 2011
May, 2011
April, 2011
March, 2011
February, 2011
January, 2011
December, 2010
November, 2010
October, 2010
September, 2010
August, 2010
July, 2010
June, 2010
May, 2010
April, 2010
March, 2010
February, 2010
January, 2010
December, 2009
November, 2009
October, 2009
September, 2009
August, 2009
July, 2009
June, 2009
May, 2009
April, 2009
March, 2009
February, 2009
January, 2009
December, 2008
November, 2008
October, 2008
September, 2008
August, 2008
July, 2008
June, 2008
May, 2008
April, 2008
March, 2008
February, 2008
January, 2008
December, 2007
November, 2007
October, 2007
September, 2007
August, 2007
July, 2007
June, 2007
May, 2007
April, 2007
March, 2007
February, 2007
January, 2007
December, 2006
November, 2006
October, 2006
September, 2006
August, 2006
July, 2006
June, 2006
May, 2006
April, 2006
March, 2006
February, 2006
January, 2006
December, 2005
November, 2005
October, 2005
September, 2005
August, 2005
July, 2005
June, 2005
May, 2005
April, 2005
March, 2005
February, 2005
January, 2005
December, 2004
February, 2004
January, 2004
December, 2003
November, 2003
October, 2003
September, 2003
August, 2003
July, 2003
June, 2003
May, 2003
April, 2003
Monday, April 01, 2013
Dr. David Hawkins
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Sometimes life comes at you with so much, you become unsure of yourself and even begin to wonder,
Am I losing my mind? Do other people have thoughts like mine, or am I the only one
Dr. David Hawkins
spends the hour with Lynne talking about his book
Normal People Do the Craziest Things
(Harvest House, ISBN# 978-0736924788). The good news is…you're probably a lot more normal than you think, and Dr. Hawkins' insights can give you tools to manage your emotions, your stress, and even your friendships! We'll open the phones, email, and Facebook for questions, too!
Tags: n/a
Friday, March 29, 2013
"People Can't Drive You Crazy if You Don't Give Them the Keys"
Lynne FOrd
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Do you feel like everyone around you is bent on making you miserable? Is someone trying to hijack your emotions? How about the one who thrives on drama? If you could just "fix" that person, everything would be better. But we can't fix other people. We can only make choices about ourselves. In today's encore
, communication expert
Mike Bechtle
spends the hour explaining how to stop being a victim of other people's craziness with commonsense wisdom and proactive advice. There will always be difficult people. But this fresh perspective on dealing with them can change your life – starting today. Learn how
People Can't Drive You Crazy if You Don't Give Them the Keys
(Revell, ISBN# 978-0-8007-2111-4).
Tags: n/a
Thursday, March 28, 2013
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Easter holds both poignant and fun traditions for so many. What are some of your fondest Easter memories…with your family, with your church, with something that drew you closer to the Lord as you celebrated His Resurrection? Lynne and Bekah share their treasured Easter memories with you and then open the phones, email and Facebook for you to do the same!
Tags: n/a
Wednesday, March 27, 2013
Michael Card
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
The Gospel of Mark is a book of action and passion. Events happen one after another, with a vivid sense of immediacy and urgency. Jesus' emotions come through strongly - from anger to deep compassion.
Michael Card's
lively tour of
Mark: the Gospel of Passion
(IVP, ISBN# 978-0-8308-3813-4) shows glimpses into the life and ministry of Jesus. He also encourages Christians to meet our Savior at a cross - that we might understand the enormity of
A Violent Grace
(IVP, ISBN# 978-0830837724). Don't miss Lynne's hour with this compelling and insightful author.
Tags: n/a
Tuesday, March 26, 2013
"Shepherding Women in Pain"
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Infertility, divorce, domestic violence and eating disorders have all become part of a day's work for those ministering to women. Yet most are not prepared for ministering to women in real pain over real issues.
Bev Hislop
knows something about
Shepherding Women in Pain
(Moody, ISBN# 978-0-8024-7705-7) and during her visit to
, she talks with Lynne about how to give leaders and caregivers a greater understanding for hurting women. Bev encourages leaders to reach out in grace to help women deal constructively with the trauma of their life experiences.
Tags: n/a
Monday, March 25, 2013
Kitchen Caboodle
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Ever wish you could simplify your cooking and grocery shopping experience? Heather Brown from
has a great suggestion for how to do just that! Katie Farrell knows what it's like to struggle with weight, healthy eating, and seeing yourself as the Lord sees you. Her blog ministry,
Dashing Dish
, helps women cook and exercise in ways that allow their bodies to be what God created. Lynne chats with Benita Long, whose book
Share the Bounty
(Thomas Nelson, ISBN# 978-1-4016-0453-0) inspires readers to open their homes and reflect God's grace to all who sit at the table. She also talks with
Aran Goyoaga
, a food stylist whose book
Small Plates and Sweet Treats
(Little, Brown, ISBN# 978-0-316-18745-9) explores gluten free cooking! Bekah shares some kabob inspirations, and we'll have fun giveaways!
Tags: n/a
Friday, March 22, 2013
Open Phones
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
We had a bit of an impromptu open phones show...sort of a "Bekah/Lynne
if you will! If you missed it - catch random stories by Bekah and Lynne, as well as your thoughts on
The Bible
series on TV...today's fashion, and a shout-out to our own dear Doris Billiard! And if you came here looking for the show with J. Warner Wallace about
Cold Case Christianity...
we are working hard to reschedule it!
Tags: n/a
Thursday, March 21, 2013
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Well you all LOVE your ice cream, and we
have spent the entire hour discussing it last week! So this week…we ask…of all the fashion decades from years gone by, which ones would you have LOVED to live in? (Imagining corsets and petticoats will be high on the list for most of you.) Or maybe you want to tell us what fashion fad you
miss! Spend the hour with us for some fashion fun with the former-wearers-of-massive-shoulder-pads: Lynne and Bekah.
Tags: n/a
Wednesday, March 20, 2013
Spots and Spotlights
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
We kick off the hour talking with
Jenny Simmons
, the artist featured at
WBCL's Daddy Daughter Date Night
next month!
Holley Gerth
, popular blogger, writer for Dayspring and author of
You're Made for a God-Sized Dream
(Revell, ISBN# 978-0-8007-2061-2) joins us to talk about how {e)coaching might help you in the story God is writing in your life. Amy Pankratz, mastermind behind
, tells the story of this project that became bigger than she ever imagined! Dr. Chuck Gerba, aka "Dr. Germ" gives great practical advice in the ever-lingering cold and flu season of the spring. And if you find yourself in need of a charge for your phone right about the time you have no power outlet, don't miss our interview with Jeff Barlow of
Tags: n/a
Tuesday, March 19, 2013
Mitch Kruse
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Have you ever entrusted yourself to a mentor who wanted you to be more oriented toward man-made rules than relationships? Rather than basing advice on the teaching of Christ and the counsel of the Bible, did this mentor develop a personal potpourri of teachings? Jesus addressed this in the Sermon on the Mount when He taught His disciples how to go with discretion to their teachers and leaders. Tuesday,
Mitch Kruse
and Lynne unpack this section of teaching and take your questions!
Tags: n/a
Monday, March 18, 2013
Author! Author!
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Author! Author! starts out with an Easter feel:
Bodie and Brock Thoene's
new fiction series "The Jerusalem Chronicles" starts with
When Jesus Wept
(Zondervan, ISBN# 978-0310335931).
ystal Bowman's
children's book
Lily's Easter Party
(Zondervan, ISBN# 978-0-310-72595-4) tells the story of the Resurrection Eggs. We'll also talk with Mike Berenstain, author of the well-loved
Berenstain Bears Storybook Bible
(Zondervan, ISBN# 978-0-310-72721-7). Fiction author
Joanne Bischof
chats with us about her Appalachian romance,
Though My Heart is Torn
(Multnomah, ISBN # 978-1-60142-423-5), and
Lisa Williams Kline
introduces her newest book in young adult fiction "Sisters in All Seasons" series:
Winter's Tide
(Zondervan, ISBN#978-0-310-72619-7). And we wrap up the hour with Dale Larsen, who along with his wife, Sandy, co-authored
Living Your Legacy
(IVP, ISBN# 978-0-8308-2113-6), an action packed guide for the later years!
Tags: n/a
Friday, March 15, 2013
Dr. Patricia Raymond
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
She's baaaaaaaaaaack!! Gastroenterologist
Dr. Patricia Raymond
is more than just a doctor with a difficult-to-pronounce specialty. The speaker, author, humorist and self-proclaimed "diva of doo" returns to
with her medicine bag full of colonoscopy jokes, parodies and more...to share helpful tips, ease your fears, and take your questions! You won't want to miss her Entertaining and informative visit to
Tags: n/a
Thursday, March 14, 2013
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Bekah and Ryan celebrated their marriage with all their friends and family this past weekend during their at-home reception, which featured an ice cream sundae buffet. We'd love to hear your favorite ice cream concoction! And while thinking about fashion after consuming an ice cream sundae may not be a logical transition…we would also love to hear which decade's fashion tickles your fancy…if you could go back to live in any other period in fashion history!
Tags: n/a
Wednesday, March 13, 2013
"The Sacred Search"
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
What if you stopped looking for "a soul mate" and started looking for "a sole mate" - someone who will live out with you the great purpose of God? What if dating isn't about finding "the one" but making a wise choice so you can better serve the One who loves you most? What if God didn't design relationships to make you happy, but to make you holy? Respected author,
Gary Thomas
talks with Lynne about a transforming way to look at romantic relationships. A good marriage isn't something you find...it's something you make through
The Sacred Search
(Cook, ISBN# 978-1-4347-0489-4).
Tags: n/a
Tuesday, March 12, 2013
Dr. Ann Kulze
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Did you know it takes 30 minutes of jogging to work off a candy bar?
Dr. Ann Kulze
and Lynne rethink some common snacks from the standpoint of exercise…and they talk about the potential stroke hazard of a steady diet of "southern foods" – like fried chicken and sweet tea. And if you're a caffeine lover…don't miss their discussion on surprising benefits of caffeine! We'll also open our phone lines, email, and Facebook for you to pose your nutrition question to Dr. Ann!
Tags: n/a
Monday, March 11, 2013
People to People
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Mark Schultz
is well known for his music, but he also has a powerful personal testimony that began when he was adopted at birth. He's coming to Fort Wayne, this time not to give a concert, but to share his story at
A Hope Center's
banquet, and Lynne will talk to him about his passion for life and adoption. And in the second half of the show, Lynne talks to
Dave Moore
, author of
The Father's Love
(Tate, ISBN# 978-1-61739-369-3) who shares the true story of his cross-country search for his missing son.
Tags: n/a
Friday, March 08, 2013
"How to Survive the End of the World"
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Does the phrase "end of the world" scare you half to death?
Bob Hostetler
gets that. The author of
How to Survive the End of the World
(Leafwood ISBN# 978-0891123255) takes the typical "dark and dismal catastrophe" topic and tackles it in a fresh, invigorating way. Without using any theories or timelines, he takes the Bible's revelation of living in the end times the way it was meant to be seen: as a survivor's guide…an encouraging and equipping message that will prepare you for the end, whenever and however it may come.
Tags: n/a
Thursday, March 07, 2013
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
If you can survive your junior year of high school, you can survive anything…or so Jay Mathews said in his guide to surviving 11th grade…which is still handed out in some public schools seven years later. What year of school put you in survival mode? We share ours and chat with you about yours. And along the way, we'll have stories, Pinterest inspirations, funny tweets and more!
Tags: n/a
Wednesday, March 06, 2013
"Surfing for God"
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
In a world where there are 68 million searches for pornography every day and where over 70% of Christian men report viewing porn in the last year, it's no surprise that more and more men struggle with an addiction to this false fantasy. Counselor and ministry leader Michael John Cusick joins Lynne to show us how the pursuit of empty pleasure is really a search for our heart's deepest desire – and the real key to resistance is discovering and embracing the joy we truly want. Backed by Scripture, personal and professional experience, he helps us understand how porn struggles begin, how to prevent them ,and how to overcome the compulsion once it begins and most of all, the joy of
Surfing for God
(Thomas Nelson, ISBN# 978-0-8499-4723-0).
Tags: n/a
Tuesday, March 05, 2013
Kerri Zurbuch
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
How well are you aging? Wellness consultant and personal fitness trainer
Kerri Zurbuch
has the revealing quiz on an hour packed with get healthy advice: this month's Healthy Habit; good-for-you recipes (including a white bean and chorizo soup); Fitness Finds (Pick and Pull exercises you only do ONCE a week); plus our regular Q & A for answers to your fitness questions.
Tags: n/a
Monday, March 04, 2013
Dr. David Hawkins
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Dr. David Hawkins
answers your life and relationship questions, he has a few things on his mind to unpack: how thinking like a man can boost a woman's well-being; how a "third eye" brings the impact of your words into focus; plus important advice for women married to narcissistic men. Call, Facebook or e-mail your questions beginning at 10:25.
Tags: n/a
Friday, March 01, 2013
"The MoneySmart Family System"
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
New York Times
best-selling authors
Steve and Annette Economides
raised five children while spending 77% less than the USDA predicted. And the money they did spend was used to train their children to become financially independent. The authors of
The MoneySmart Family System
will show you how to teach your children to manage money and have a good attitude while learning to earn, budget and spend wisely. Join their conversation with Lynne to learn how to inspire your kids to help with chores, prepare them for a paying job, pay for their own cars and insurance, and employ strategies for debt-free education!
Tags: n/a
Thursday, February 28, 2013
Lynne Ford and Bekah Shaffer
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Come on…you can tell us. What piece of clothing did you really, REALLY,
need to throw away a while ago but you just can't do it! We won't judge – but we can't promise not to giggle a little. Spend the hour with us for some fun stories, great laughs, and conversations with all of YOU!!!
Tags: n/a
Wednesday, February 27, 2013
"A Season of Breakthrough"
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
Do you have some freedom stealers lurking in your life? Have you experienced some breakthroughs...only to find yourself soon struggling with the same pitfalls? Author and speaker
Paula Friedrichsen
joins us for an hour to explain not only how to experience breakthrough...but how to walk in
sustained freedom
. She shares her testimony of healing from migraines and medication dependency and offers strategies for
A Season of Breakthrough
(ISBN# 9780967750880) ...found in risky obedience, tenacious faith, awareness, and transforming love!
Tags: n/a
Tuesday, February 26, 2013
"Dating and the Single Parent"
Lynne Ford
Listen Now
Number of listens: 0
Download File
Number of downloads: 0
When kids are involved...dating gets complicated. Single mom
PeggySue Wells
and author
Ron Deal
spend the hour with Lynne, offering advice on how to determine if you're
ready to date and how to deal with your kids' emotions as you embark on this new journey. They also have insights on what to expect when dating as a parent - and how to make sure you still have plenty of time to focus on your children. If you think you're ready to start dating again, don't miss this conversation on
Dating and the Single Parent
(Bethany House, ISBN# 978-0-7642-0697-9) that can help you see beyond tingly feelings to prepare for life as a healthy, blended family.
Tags: n/a
10 Items Per Page
20 Items Per Page
25 Items Per Page
50 Items Per Page
100 Items Per Page
« Prev
Next »
Afternoons with Scott
Program Schedule
What's Playing Now
Culture Connection
The WBCL Radio Network | ALL RIGHTS RESERVED |
Connect with us
Website Development by Cirrus ABS


text:
Join OkCupid
Find better matches with our advanced
Find better matches with our advanced
matching system
—% Match
—% Enemy
26 • Brooklyn, NY • Woman
Tests they've taken (4)
|Their result
|Your result
|The Dating Persona Test
|The Wild Rose (RBLD)
|Take it!
|The Slut Test
|64% slut
|Take it!
|The Virgin Game
|57% Accuracy (highest: 57%, average: 57%)
|Take it!
|The How Annoying Is Your Profile? Test
|Not Annoying
|Take it!
Connect your existing OkCupid account
Sign in
Welcome Back!
Restore your account to continue meeting new people.
Reset your password
We'll email you a link to reset it.
Email sent!
An email is on its way to . If you don't see it, try checking your Spam folder.


text:
Two people were trapped following a collision between a van and a car in Gravesend this afternoon.
The pair had to be freed by firefighters using hydraulic rescue equipment after the crash in Milton Road at around 1.30pm.
Fire crews, police and ambulance all attended and one woman in her 80s and one man were taken to hospital.
The woman was suffering from chest pains and was taken to Darent Valley Hospital while the man was taken to Medway Maritime Hospital.
The road was briefly closed to allow emergency services access.
Did you see what happened? Send your pictures to email@example.com
- Motorists can now challenge parking tickets for £5
- Gravesend prankster banned from football matches for three years after Tottenham Hotspur pitch invasion
- UPDATE: Man rushed to hospital after car flips over on the A2 near Darenth
- 5 Thoughts: Travellers fighting back, another UKIP gaffe and more
- Man cut free from van after Gravesend crash


text:
See our store section for gifts by different stores
See our top gift ideas section for most popular items.
Create your own personal gift registry. Register for any item, from any store, for any occasion. Find the gift registries of your family and friends.
Ever find the perfect gift idea for someone, but their birthday was months away? We make it easy to remember your gift ideas.
Provides timely email reminders for a birthday, anniversary, and other special occasions.
Get advice from friends and family on which gifts to buy.
Bradford Exchange
Splendor in the Sky Illuminated Tabletop Sculpture
Thomas Kinkade You Are My Sweet-Tea Figurine
On Eagle's Wings Sculpture with Scripture
Spirit Of Benitoite Eagle Figurine
Owl Always Love You Owl Figurine
Cold-Cast Bronze Bald Eagle Wall Sculpture
Lena Liu Precious Treasure Hummingbird Music Box
Together We Stand, United We Soar Eagle Figurine
Embroidered Men's Leather Biker Jacket
We Will Never Forget Patriotic Eagle Sculpture
Wisdom of the Amethyst Purple Owl Figurine On Mirror
American Spirit Ford F1 Truck Sculpture
Timeless Majesty Cuckoo Clock with Bald Eagle Art
Blossoms and Hummingbirds Lamp
Soaring Spirits Eagle Art Wall Decor
Woodland Guardian Owl Sculpture
John Wayne Patriotic Commemorative American Eagle Wall Sculpture
Pretty as a Peacock Handbag
Sunrise Cascade Eagle Sculpture
Cardinal Serenade Hand-Blown Glass Bowl
Invoking the Sacred Guardian Sculpture
Angel of Fluttering Renewal Figurine
Majestic Retreat Personalized Masterpiece Framed Plate
Canyon Guardian Eagle Art Sculpture
Handmade in the USA! Colorful Birds on Wood Bookmarks


text:
A foreign exchange specialist that saves you money and makes a difference.
If you've stumbled across this site, chances are you'll already have a vague idea what a foreign exchange specialist does. In a nutshell, we work with individuals, businesses, charities and NGOs, beating high street bank rates to save them up to 4% on their currency transactions.
Hopefully, a 4% saving will have already piqued your interest. But this particular foreign exchange firm stands out in more ways than one. Why? Because we're the first foreign exchange broker to give a percentage of our hard-earned profits to help good causes globally, at no extra cost to our clients.
That's why we call ourselves Ethical Currency. Now if everyone in our industry followed our lead (chance would be a fine thing), then between $30 billion and $40 billion could be raised every year. We call it change through exchange.
We've also taken steps to make foreign exchange simpler. Instead of having to manage multiple accounts to transact in different currencies, all our clients get just one, free multi-currency account from which they can make and receive international currency payments in more than 40 currencies. We find that speeds things up, and makes things a lot less complicated.
One Account –
Global Reach
Using just one, free, no obligations account you can receive, transact between & make payments in over forty currencies.
Currency Transactions
& Hedging
Ethical Currency trades in the interbank FX markets. That means we access wholesale currency rates which we can pass on to our clients, offering them... Find out more


text:
It is often those without the means that develop the urge to share their meager resources with the needy. Earlier, their efforts often went like irrigation in a patch of desert. But with technology like Facebook, the tiny group with keen ideal can attract worldwide supporters for their cause, as Vinod Bhaskar, conductor of KSRTC in the Changanassery – Alappuzha route has done.
Vinod does not even have a house of his own. But he had heard and experienced the noble ideal of his father in marrying his mother, who was a polio-stricken cripple. That large-heartedness was the inspiration for him. In 2011, he formed a Facebook group to called 'V Help'. This group went about helping the needy in terms of cash, medicine and implements.
As the group went about its activities, it became aware of the demand for blood donation. Seeing the need and response, a new group 'Blood Donors Kerala' was formed. It was not long before the group was asked to be on its toes. Daily about 70 requests reach the group. Vinod says that it is the strength of the group that they are able to cater to the whole demand.
There are 60 active members in the group. Worldwide support-base sustain the group and it is able to serve throughout Kerala.
On Sundays he leaves his rented house with home-cooked food to serve the needy in the streets. Vinod uses all his spare time for such humanitarian activities. His wife Usha is his stable support at home.
Courtesy: Mathrubhumi, August 21, 2014
"If you cannot do great things, do small things in a great way."