text: Virology Journal - Latest Articles http://www.virologyj.com The latest research articles published by Virology Journal 2014-12-19T12:00:00Z Inhibition of porcine reproductive and respiratory syndrome virus infection by recombinant adenovirus- and/or exosome-delivered the artificial microRNAs targeting sialoadhesin and CD163 receptors Background: The current vaccines failed to provide substantial protection against porcine reproductive and respiratory syndrome (PRRS) and the new vaccine development faces great challenges. Sialoadhesin (Sn) and CD163 are the two key receptors for PRRS virus (PRRSV) infection of porcine alveolar macrophages (PAMs), but the artificial microRNA (amiRNA) strategy targeting two viral receptors has not been described. Methods: The candidate miRNAs targeting Sn or CD163 receptor were predicted using a web-based miRNA design tool and validated by transfection of cells with each amiRNA expression vector plus the reporter vector. The amiRNA-expressing recombinant adenoviruses (rAds) were generated using AdEasy Adenoviral Vector System. The rAd transduction efficiencies for pig cells were measured by flow cytometry and fluorescent microscopy. The expression and exosome-mediated secretion of amiRNAs were detected by RT-PCR. The knock-down of Sn or CD163 receptor by rAd- and/or exosome-delivered amiRNA was detected by quantitative RT-PCR and flow cytometry. The additive anti-PRRSV effect between the two amiRNAs was detected by quantitative RT-PCR and viral titration. Results: All 18 amiRNAs validated were effective against Sn or CD163 receptor mRNA expression. Two rAds expressing Sn- or CD163-targeted amiRNA were generated for further study. The maximal rAd transduction efficiency was 62% for PAMs at MOI 800 or 100% for PK-15 cells at MOI 100. The sequence-specific amiRNAs were expressed efficiently in and secreted from the rAd-transduced cells via exosomes. The expression of Sn and CD163 receptors was inhibited significantly by rAd transduction and/or amiRNA-containing exosome treatment at mRNA and protein levels. Both PRRSV ORF7 copy number and viral titer were reduced significantly by transduction of PAMs with the two rAds and/or by treatment with the two amiRNA-containing exosomes. The additive anti-PRRSV effect between the two amiRNAs was relatively long-lasting (96?h) and effective against three different viral strains. Conclusion: These results suggested that Sn- and CD163-targeted amiRNAs had an additive anti-PRRSV effect against different viral strains. Our findings provide new evidence supporting the hypothesis that exosomes can also serve as an efficient small RNA transfer vehicle for pig cells. http://www.virologyj.com/content/11/1/225 Li Zhu Hongqin Song Xinyu Zhang Xiaoli Xia Huaichang Sun Virology Journal 2014, null:225 2014-12-19T12:00:00Z doi:10.1186/s12985-014-0225-9 /content/figures/s12985-014-0225-9-toc.gif Virology Journal 1743-422X ${item.volume} 225 2014-12-19T12:00:00Z PDF Clearly different mechanisms of enhancement of short-lived Nef-mediated viral infectivity between SIV and HIV-1 Background: One of the major functions of Nef is in the enhancement of the infectivity of the human and simian immunodeficiency viruses (HIV and SIV, respectively). However, the detailed mechanism of the enhancement of viral infectivity by Nef remains unclear. Additionally, studies of mechanisms by which Nef enhances the infectivity of SIV are not as intensive as those of HIV-1. Methods: We generated short-lived Nef constructed by fusing Nef to a proteasome-mediated protein degradation sequence to characterize the Nef role in viral infectivity. Results: The apparent expression level of the short-lived Nef was found to be extremely lower than that of the wild-type Nef. Moreover, the expression level of the short-lived Nef increased with the treatment with a proteasome inhibitor. The infectivity of HIV-1 with the short-lived Nef was significantly lower than that with the wild-type Nef. On the other hand, the short-lived Nef enhanced the infectivity of SIVmac239, an ability observed to be interestingly equivalent to that of the wild-type Nef. The short-lived Nef was not detected in SIVmac239, but the wild-type Nef was, suggesting that the incorporation of Nef into SIVmac239 is not important for the enhancement of SIVmac239 infectivity. Conclusions: Altogether, the findings suggest that the mechanisms of infectivity enhancement by Nef are different between HIV-1 and SIVmac239. Lastly, we propose the following hypothesis: even when the expression level of a protein is extremely low, the protein may still be sufficiently functional. http://www.virologyj.com/content/11/1/222 Keisuke Harada Nobutoki Takamune Shozo Shoji Shogo Misumi Virology Journal 2014, null:222 2014-12-18T12:00:00Z doi:10.1186/s12985-014-0222-z /content/figures/s12985-014-0222-z-toc.gif Virology Journal 1743-422X ${item.volume} 222 2014-12-18T12:00:00Z PDF Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia Background: Fevers of unknown origin constitute a substantial disease burden in Southeast Asia. In majority of the cases, the cause of acute febrile illness is not identified. Methods: We used MassTag PCR, a multiplex assay platform, to test for the presence of 15 viral respiratory agents from 85 patients with unexplained respiratory illness representing six disease clusters that occurred in Cambodia between 2009 and 2012. Results: We detected a virus in 37 (44%) of the cases. Human rhinovirus, the virus detected most frequently, was found in both children and adults. The viruses most frequently detected in children and adults, respectively, were respiratory syncytial virus and enterovirus 68. Sequence analysis indicated that two distinct clades of enterovirus 68 were circulating during this time period. Conclusions: This is the first report of enterovirus 68 in Cambodia and contributes to the appreciation of this virus as an important respiratory pathogen. http://www.virologyj.com/content/11/1/224 Nary Ly Rafal Tokarz Nischay Mishra Stephen Sameroff Komal Jain Agus Rachmat Ung An Steven Newell Dustin Harrison W Lipkin Virology Journal 2014, null:224 2014-12-17T12:00:00Z doi:10.1186/s12985-014-0224-x /content/figures/s12985-014-0224-x-toc.gif Virology Journal 1743-422X ${item.volume} 224 2014-12-17T12:00:00Z PDF Kaposi¿s sarcoma-associated herpesvirus G-protein coupled receptor activates the canonical Wnt/ß-catenin signaling pathway Background: KSHV is a tumorigenic ?-herpesvirus that has been identified as the etiologic agent of Kaposi?s sarcoma (KS), a multifocal highly vascularized neoplasm that is the most common malignancy associated with acquired immunodeficiency syndrome (AIDS). The virus encodes a constitutively active chemokine receptor homologue, vGPCR that possesses potent angiogenic and tumorigenic properties, and is critical for KSHV pathobiology. To date, a number of signaling pathways have been identified as key in mediating vGPCR oncogenic potential.FindingsIn this study, we identify a novel pathway, the Wnt/?-catenin pathway, which is dysregulated by vGPCR expression in endothelial cells. Expression of vGPCR in endothelial cells enhances the nuclear accumulation of ?-catenin, that correlates with an increase in ?-catenin transcriptional activity. Activation of ?-catenin signaling by vGPCR is dependent on the PI3K/Akt pathway, as treatment of vGPCR-expressing cells with a pharmacological inhibitor of PI3K, leads to a decreased activation of a ?-catenin-driven reporter, a significant decrease in expression of ?-catenin target genes, and reduced endothelial tube formation. Conclusions: Given the critical role of Wnt/?-catenin signaling in angiogenesis and tumorigenesis, the findings from this study suggest a novel mechanism in KSHV-induced malignancies. http://www.virologyj.com/content/PREACCEPT-3896552291322069 Magdalena Angelova MaryBeth Ferris Kenneth Swan Harris McFerrin Gabriella Pridjian Cindy Morris Deborah Sullivan Virology Journal 2014, null:218 2014-12-17T12:00:00Z doi:10.1186/PREACCEPT-3896552291322069 Novel mechanism in herpesvirus-induced malignancies Kaposi's sarcoma-associated herpesvirus was found to dysregulate the Wnt/beta-catenin pathway within endothelial cells, which might be a novel mechanism in herpesvirus-induced malignancies. /content/figures/s12985-014-0218-8-toc.gif Virology Journal 1743-422X ${item.volume} 218 2014-12-17T12:00:00Z PDF The SARS coronavirus papain like protease can inhibit IRF3 at a post activation step that requires deubiquitination activity Background: The outcome of a viral infection is regulated by complex interactions of viral and host factors. SARS coronavirus (SARS-CoV) engages and regulates several innate immune response pathways during infection. We have previously shown that the SARS-CoV Papain-like Protease (PLpro) inhibits type I interferon (IFN) by inhibiting IRF3 phosphorylation thereby blocking downstream Interferon induction. This finding prompted us to identify other potential mechanisms of inhibition of PLpro on IFN induction. Methods: We have used plasmids expressing PLpro and IRF3 including an IRF3 mutant that is constitutively active, called IRF3(5D). In these experiments we utilize transfections, chromatin immunoprecipitation, Electro-mobility Shift Assays (EMSA) and protein localization to identify where IRF3 and IRF3(5D) are inhibited by PLpro. Results: Here we show that PLpro also inhibits IRF3 activation at a step after phosphorylation and that this inhibition is dependent on the de-ubiquitination (DUB) activity of PLpro. We found that PLpro is able to block the type I IFN induction of a constitutively active IRF3, but does not inhibit IRF3 dimerization, nuclear localization or DNA binding. However, inhibition of PLpro's DUB activity by mutagenesis blocked the IRF3 inhibition activity of PLpro, suggesting a role for IRF3 ubiquitination in induction of a type I IFN innate immune response. Conclusion: These results demonstrate an additional mechanism that PLpro is able to inhibit IRF3 signaling. These data suggest novel innate immune antagonism activities of PLpro that may contribute to SARS-CoV pathogenesis. http://www.virologyj.com/content/11/1/209 Krystal Matthews Alexandra Schäfer Alissa Pham Matthew Frieman Virology Journal 2014, null:209 2014-12-07T12:00:00Z doi:10.1186/s12985-014-0209-9 /content/figures/s12985-014-0209-9-toc.gif Virology Journal 1743-422X ${item.volume} 209 2014-12-07T12:00:00Z XML The diversity of human papillomavirus infection among human immunodeficiency virus-infected women in Yunnan, China Background: Yunnan has one of the oldest and the most severe human immunodeficiency virus (HIV) epidemics in China. We conducted an observational study to evaluate the human papillomavirus (HPV) genotype distribution in relation to cervical neoplastic disease risk among HIV-infected women in Yunnan. Methods: We screened 301 HIV-infected non-pregnant women in Mangshi prefecture in Yunnan province. All consenting participants underwent simultaneous and independent assessment by cervical cytology, colposcopy-histopathology, and HPV genotyping. Unadjusted and multivariable-adjusted multinomial logistic regression analysis was conducted to evaluate factors associated with single or multiple carcinogenic HPV genotypes. Results: HPV genotypes were present in 43.5% (131/301) overall, and carcinogenic HPV genotypes were present in 37.5% (113/301) women. Among women with carcinogenic HPV genotypes, 80 (70.8% of 113) had a single carcinogenic HPV type, while 33 (29.2%) women had multiple (2 or more) carcinogenic HPV types. Overall, the most common carcinogenic HPV types were HPV52 (7.3%), HPV58 (6.6%), HPV18 (6.3%), HPV16 (6.0%), and HPV33 (5.3%). In women with cervical precancerous lesions (i.e., high-grade squamous intraepithelial lesions [HSIL] on cytology or cervical intraepithelial neoplasia grades 2 or worse [CIN2+] detected on colposcopy-histology), the most commonly detected genotypes were HPV16 (28.6%), HPV52 (25.0%), HPV58 (17.9%), HPV18 (10.7%) and HPV31 (10.7%). Increasing age was an independent risk factor associated with presence of single carcinogenic HPV types (adjusted odds ratio: 1.04, 95%CI: 1.01-1.07, p?=?0.012) but not with the presence of multiple carcinogenic types in the multivariable-adjusted models. Conclusions: As HIV-infected women continue to live longer on antiretroviral therapy in China, it will be increasingly important to screen for, and prevent, HPV-associated cervical cancer in this population, especially given the wide diversity and multiplicity of HPV genotypes. http://www.virologyj.com/content/PREACCEPT-1753047692124784 Hong-Yun Zhang Man-Dong Fei Yong Jiang Qiu-Yue Fei Hong Qian Lin Xu Yu-Ni Jin Cheng-Qin Jiang Hai-Xia Li Sarah Tiggelaar Jennifer Smith Vikrant Sahasrabuddhe You-Lin Qiao Virology Journal 2014, null:202 2014-12-07T12:00:00Z doi:10.1186/PREACCEPT-1753047692124784 HPV infection in HIV-infected women in China Human papillomavirus genotypes were found in 43.5% HIV-infected women in Yunnan province, China, highlighting the importance to screen for and prevent HPV in this population. /content/figures/s12985-014-0202-3-toc.gif Virology Journal 1743-422X ${item.volume} 202 2014-12-07T12:00:00Z PDF pH-Dependent entry of chikungunya virus fusion into mosquito cells Background: Millions of human infections caused by arthropod-borne pathogens are initiated by the feeding of an infected mosquito on a vertebrate. However, interactions between the viruses and the mosquito vector, which facilitates successful infection and transmission of virus to a subsequent vertebrate host, are still not fully understood.FindingHere we describe early chikungunya virus (CHIKV) infectious events in cells derived from one of the most important CHIKV vectors, Aedes albopictus. We demonstrated that CHIKV infection of mosquito cells depended on acidification of the endosome as indicated by significant inhibition following prophylactic treatment with the lysosomotropic drugs chloroquine, ammonium chloride, and monensin, which is consistent with observations in mammalian cells. While all three agents inhibited CHIKV infection in C6/36 cells, ammonium chloride was less toxic to cells than the other agents. Conclusion: The observation of similar mechanisms for inhibition of CHIKV infection in mosquito and mammalian cell lines suggests that conserved entry pathways are utilized by CHIKV for vertebrate and invertebrate cell types. http://www.virologyj.com/content/11/1/215 John Nuckols Alexander McAuley Yan-Jang Huang Kate Horne Stephen Higgs Robert Davey Dana Vanlandingham Virology Journal 2014, null:215 2014-12-05T00:00:00Z doi:10.1186/s12985-014-0215-y /content/figures/s12985-014-0215-y-toc.gif Virology Journal 1743-422X ${item.volume} 215 2014-12-05T00:00:00Z XML E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein Background: In HIV-1 infected patients, production of interleukin-10 (IL-10), a highly immunosuppressive cytokine, is associated with progression of infection toward AIDS. HIV-1 Tat protein, by interacting with TLR4-MD2 at the membrane level, induces IL-10 production by primary human monocytes and macrophages. In the present study we evaluated the effect of the TLR4 antagonist Eritoran tetrasodium (E5564) on HIV-1 Tat-induced IL-10 production.FindingsHere, we confirm that the recombinant HIV-1 Tat protein and the GST-Tat 1–45 fusion protein efficiently stimulate IL-10 production by primary monocytes and macrophages and that this stimulation is inhibited by blocking anti-TLR4 mAbs. We show that a similar inhibition is observed by preincubating the cells with the TLR4 antagonist E5564. Conclusion: This study provides compelling data showing for the first time that the TLR4 antagonist E5564 inhibits the immunosuppressive cytokine IL-10 production by primary human monocytes and macrophages incubated in the presence of HIV-1 Tat protein. http://www.virologyj.com/content/11/1/214 Elmostafa Bahraoui Laurence Briant Nathalie Chazal Virology Journal 2014, null:214 2014-12-04T12:00:00Z doi:10.1186/s12985-014-0214-z /content/figures/s12985-014-0214-z-toc.gif Virology Journal 1743-422X ${item.volume} 214 2014-12-04T12:00:00Z XML Characterization of PERV in a new conserved pig herd as potential donor animals for xenotransplantation in China Background: Xenotransplantation has drawn increased attention in recent years as a potential solution to the scarcity of human source donor organs. Researchers have highlighted the need to characterize the influence of porcine endogenous retroviruses (PERV) in xenotransplantation. Screening and analyzing the presence and subtype of PERV in donor source animal breeds could provide basic parameters to evaluate the biological safety of xenotransplantation from pigs to humans. We bred a new miniature porcine herd (XENO-1) after decades of investigation, the herd was purpose bred to produce a potential donor animal source for xenotransplantation. To this end we studied the animals' PERV expression characteristics. Methods: We randomly selected 37 animals of the herd, PCR and RT-PCR based on specific primers were utilized to determine their PERV viral subtype. High fidelity PCR and restriction enzyme digestion were employed for variants detection. To thoroughly understand the PERV expression pattern, quantitative PCR was applied to measure mRNA expression levels in different tissues, At last, transfection capacity was assessed using a in vitro co-culture system. Results: Our results revealed that the XENO-1 herd was free of PERV-C and exhibited low levels of PERVs in different tissues compared to commercial pig (landrace). The XENO-1 herd showed unique variants of A/B recombination. In addition, even though there were A/B variants in the XENO-1 herd, co-culturing revealed no evidence of PERV transmission from XENO-1 tissue to human cells. Conclusion: Overall, Our results displayed an unique PERV expression pattern in a new pig herd and demonstrated its non-transfection capacity in vitro. Data in the research indicate that XENO-1 animals can serve as a better potential donor source for xenotransplantation. http://www.virologyj.com/content/11/1/212 Fei Guo Xiaowei Xing Wayne Hawthorne Qiong Dong Bin Ye Juan Zhang Qi Liang Wei Nie Wei Wang Virology Journal 2014, null:212 2014-12-04T00:00:00Z doi:10.1186/s12985-014-0212-1 New pig herd for safe xenotransplantation Scientists in China have bred a new pig herd which is free of porcine endogenous retroviruses C, providing a better potential source of organs for human transplants. /content/figures/s12985-014-0212-1-toc.gif Virology Journal 1743-422X ${item.volume} 212 2014-12-04T00:00:00Z XML Surveillance of avirulent Newcastle disease viruses at live bird markets in Eastern China during 2008¿2012 reveals a new sub-genotype of class I virus Background: The strains of Newcastle disease virus (NDV) can be divided into two distinct clades: class I and class II. At present, limited molecular epidemiological data are available for the class I virus at live bird markets (LBMs). Knowing the genomic and antigenic characteristics of class I NDVs might provide important insights into the evolution dynamics of these viruses. In this study class I NDVs isolated from LBMs in Eastern China between 2008 and 2012 were characterized. Results: We characterized 34 class I NDVs genetically and 15 of the 34 NDVs pathologically which originated from geese, chickens and ducks at live bird markets. Based on the older classification system, twelve of fourteen strains isolated from 2008 to 2010 belonged to sub-genotype 3b. However, the rest 22 strains formed a separate novel cluster in genotype 3, which was designated as sub-genotype 3c. When based on the new classification system, sub-genotype 3b was classified into sub-genotype 1a and the sub-genotype 3c was classified into sub-genotype 1b. Over 62% (21/34) of the viruses were chicken-origin and only 13 isolates were waterfowl-origin. The Cross-neutralization reactions between CK/JS/05/11, CK/JS/06/12 and the vaccine strain LaSota showed significant antigenic differences between them. Conclusions: Currently, sub-genotype 3c (or 1b) NDVs are the most frequently isolated classI strains at LBMs in Eastern China., and the class I NDVs has transferred from waterfowls to chickens and circulated in chicken flocks extensively. http://www.virologyj.com/content/11/1/211 Jie Zhu Haixu Xu Jingjing Liu Zhenzhen Zhao Shunlin Hu Xiaoquan Wang Xiufan Liu Virology Journal 2014, null:211 2014-12-04T00:00:00Z doi:10.1186/s12985-014-0211-2 /content/figures/s12985-014-0211-2-toc.gif Virology Journal 1743-422X ${item.volume} 211 2014-12-04T00:00:00Z XML


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text: Health and Safety Services Guidance on Overnight and Long Term Running of Experiments / Equipment Introduction 1. Unplanned incidents involving long term and overnight running of experiments / equipment can pose a risk to buildings, staff, building users and members of the emergency services especially where chemicals, machinery, gases or power supplies are involved. This guidance note describes good practice in managing such risks. Guidance 2. Where experiments / equipment are intended to run overnight or for longer periods, Schools should establish clear procedures for approving arrangements and risk control measures. These should be described in the School's Health & Safety Policy Statement (in the detailed arrangements) and drawn to the attention of all staff and post-graduate students. 3. Permission to run such equipment should be obtained - BEFORE it is left to operate - from a Principal Investigator (or other specified person such as a Laboratory or School Chief Technician, or School Safety Advisor). Permission should be granted only after the production and scrutiny of a suitable and sufficient risk assessment for the work which includes an assessment of the risks associated with leaving the experiment / equipment unattended. 4. The risk assessment should describe basic details such as the location, duration, those involved in the work (and therefore knowledgeable about it), and specify : - Who is to set up the experiment / equipment, their competence and whether any special training required What fail-safe devices or alarms are required (if any) Who will respond to the activation of any alarm, and how How all services connections are to be made, tested and maintained The arrangements for regularly checking that the equipment is still operating safely over weekends, or during holiday periods when the University is shut down Any specific safety risk associated with leaving equipment running for long periods (eg overheating, drying out)? Any hazardous materials used, and what controls are in place to control exposure to them The worst-case scenario if the equipment was to fail eg explosion, fire, chemical release, risk of electrocution. Control measures necessary to protect those attending in such an emergency Any other risk that might be specific to the site or activity 5. Once the risk assessment has been approved, a permit to run experiment / equipment should be completed, and attached to the equipment. The information included on this should be clearly legible and easy for non-laboratory staff (eg Security, Fire Service crews) to understand. A pro forma is given in the Appendix. 6. Schools should establish a system for keeping copies of permits and risk assessments, so that safety personnel can monitor permit operation and cancel permits when required. The system could also provide back-up information in the event of a major emergency when access to a laboratory is restricted by the emergency services or due to a fire or explosion. (For example, School of Chemistry retain copies at a reception point separate from laboratory areas, which is permanently staffed or accessible to authorised persons.) 7. Investigation of incidents arising from the failure of unattended equipment have highlighted the following points which should be considered: The failure of equipment or services should result in a safe shut down, ie equipment should be designed to fail to a safe condition. This may mean fail- safe devices are fitted which guard against the failure of services e.g. water, electricity or gas supplies. Water connections should be screw clip or similar devices. Other connections should also be securely made. Building and University fire precautions should be observed. Stocks of hazardous materials not actually in use should be either removed from the area where the work is to take place or kept within proper stores/cabinets in the area. This especially applies to flammables, gas cylinders and hazardous chemicals. The equipment should be observed for at least one hour under the exact conditions under which it will run before being left unattended. Waste outlets should be checked at regular intervals to ensure they are clear of obstruction (frequency of checks will depend on the circumstances, and this should be stated in the risk assessment) Procedures for isolating services safely should be described in the permit attached to the equipment (so that isolation can be carried out safely, if necessary by Security or emergency services) The effect of an adverse event on other experiments / equipment in the vicinity, which could lead to additional or different risks. 8. The permit should be completed by the PI or other authorised person, and attached to equipment. Contact details and any instructions in the event of an emergency must be clearly indicated. This permit notifies Security that equipment is being left running and should not to be altered under normal circumstances. Security 9. University Security Service staff should report to Health and Safety Services any instances of equipment found running overnight without authorisation. 10. If any Security Officer encounters a problem with equipment / experiments left running overnight or for long periods they should assess the situation, and have regard to the information displayed, before taking appropriate action. Completion / Cancellation of Permit 11. On completion of the experiment or if the period of operation is exceeded then the permit should be cancelled. If work re-commences, a new permit should be obtained. 12. On completion of the experiment then the permit should be cancelled. If work re- commences, a new permit should be obtained. If the period of permit operation must be exceeded either an extension should be agreed or new permit obtained otherwise the work should cease immediately. 13. If equipment is found to be running in contravention of any specified and agreed controls such that a significant risk exists or could exist, the permit should be cancelled and this action reported to the school Health & Safety Committee. Appendix : Proforma Permit for overnight running of equipment. (NB: Existing UMIST permits can still be used) Ref No. Permit for Equipment / Experiment to Run Overnight School / Department: Location of Equipment Room Building Person Responsible Contact Details Room Tel no Details of Equipment to Run overnight: Period* (*Complete one section) PERMANENT From: (1 YEAR MAXIMUM) or From: To: To: The following services are required: - Details of services: - 1.Electricity Yes / No 2.Gas Yes / No 3.Water Yes / No 4.Steam Yes / No 5.Fume Cupboard Yes / No 6.Cylinder gases 7. 8. 9. 10. Are all service connections wired or clipped? Yes / No Are waste outlets clear of obstruction? Yes / No How frequently will the equipment / experiment be checked and by whom? Approved: Date: (Supervisor / Head of School) Out of Hours Contact Details Emergency telephone number Shut down sequence: Document control box Guidance title: Guidance on Overnight and Long Term Running of Unattended Equipment Implementation Issued November 2007 date: Version: 1.0 Supersedes: N/A Previous review N/A dates: Next review date: Upon significant change Equality impact Initial screening : low outcome Related Policies: Health & Safety Policy Related A-Z of documents on specific health & safety topics, at Procedures http://www.campus.manchester.ac.uk/healthandsafety/CoPs&Guidance.htm Related Guidance: Policy owner: Director of Health & Safety Services (Currently Dr S A Robson) Lead contact: University Safety Coordinator (Currently A. Pollitt)


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I've had occasion this past summer to deal with some actual "used to be general contractors," who currently have on the...
by Courthouserules
Unfortunately the majority of our population in the USA hasn't the foggiest
idea of how our economic and political...
by mudcat
Focus dudes, the deal is about the construction company, which is awesome by the way, but you dudes are off on a tangent.
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40,000 meals per year is about 100+ per day.
If two meals are delivered, then there must be about 50 houses fed....
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Gonna be fiyah. In just five months, the Best of the Best concert's back. Waving flags and shaking ass into downtown Miami's Bayfront Park, the annual music fest will be returning for the last wee... More >>
Who the funk is Lo Down Loretta Brown? She is Erykah Badu's DJ'ing alter ego. And she's coming for Art Basel Miami Beach week. With just days till total Basel madness, Ms. Low Down Is packing her ... More >>
Pavo. Puerco. Pumpkin pie. As residents of that fantastic land known as Miami, we are often accused of living in some foreign country where summer never ends. And yet, we give thanks for the bounty... More >>
Gratuitous tongue wagging. Thong onesies. Human-sized bananas. Where are we? An Art Basel Miami Beach rager? Or a Miley Cyrus arena show? Exactly. And that's why it is almost no surprise that Ame... More >>
In this week's Miami New Times, we profile 30 of the most interesting characters in town, with portraits of each from photographer Stian Roenning. See the entire Miami New Times People Issue here. ... More >>
After bassist Gerard Smith's 2012 death from lung cancer, TV on the Radio almost called it quits. "At the end of the last album and tour cycle I was on the page of, 'We could put this down right no... More >>
Goodbye penny beers? Over the weekend, Sandbar Sports Grill, a beloved college kid and locals hangout in Coconut Grove that's famous for its one-cent Miller Lites, was invaded by Spike TV's Bar Res... More >>
Booty, booty, booty, booty, booty, booty, booty, booty, booty. In 1989, Uncle Luke and 2 Live Crew launched a rap revolution with the release of their unrepentantly filthy slab of Miami booty bass,... More >>
The Road is rubble. Last month, the infamous century-old boozing establishment at 626 S. Miami Avenue slung its final beer and a shot. The Last Call Closing Party ended with bleary eyes and empty g... More >>
Still breathing? Good. 'Cause Look Alive Fest is back. Last year, this "homegrown, alternative festival" popped up for its inaugural, two-night Art Basel Miami Beach week edition with Detroit nois... More >>
The duke of food porn meets the godfather of punk. As Miami New Times food blog Short Order reports, Anthony Bourdain has been spotted lurking about our subtropical shores shooting a likely Miami e... More >>
Pyrite bars. And booty-quaking beats. Yep, the Fool's Gold crew is 'bout to be back in the 305 for some Basel madness. For the last several years, label boss A-Trak has regularly skedded his ratch... More >>
"Rozay, how you lose all that weight?" He once tipped the scales at 160 kilos. (Or 350 pounds for those hustlers not "in the distribution.") But big homie's recently shed the equivalent of a modest... More >>
As Bob Marley once said: "Herbs are good for every-ting." Indeed, mon. And now Momma Marley and many of Bob's children have actually gotten into the ganja-selling game. The family just launched Ma... More >>
Higher, higher, higher. As Art Basel Miami Beach week approaches, the enviable aesthetic experiences on offer just pile up, up, up. And today's thrilling #ABMB announcement... The addition of Eng... More >>
Get up. Stand up. Smoke up. Every year, in honor of Bob Marley, his legacy, and the righteousness of reggae, Jah, and ganja, the Nine Mile Music Festival livicates a full day to steady jammin', doi... More >>
Sing us a song, you're the Piano Man. It's been 20 years since Billy Joel put out a new collection of music. And he's got no plans for "another album of original tunes," he says. Maybe ever. As Jo... More >>
Crash the palace! Jump that moat, batter down the door, raid the fuckin' fridge... It's 'bout time for another epic, medieval rager at Basel Castle. After stomping the grounds of Grand Central Pa... More >>
#HeatNation... The Bawse got your back. Even though he admits LeBron is "one of the greatest, if not the greatest ever," it ain't gonna stop big homie from plotting the demise of King James and the... More >>
Infinite. Lightless. Boomin', bruh. This is The Void, where Los Angeles bass producer RL Grime just happens to be prepping his debut full-length album of the same name. It is "the deepest and dark... More >>
No more playing 'round with your heart, Basel lovers. The artsiest week of the year is almost upon us. And the latest #ABMB tidbit... Solange Knowles, Saint Records founder and Beyoncé's sis, wi... More >>
Partying is serious business. Just ask Life and Death, the Berlin-slash-NYC-slash-Miami dance music collective. For the third straight season, L&D is teaming with PL0T to throw an Art Basel Miami... More >>
"I CANNOT WAIT!" Oh yes, all caps and exclamation points... That's how excited Taylor Swift is about her 1989 World Tour. Another reason Ms. Swift is stoked: The 24-year-old record sales machine j... More >>
Shockingly and suddenly. Just an hour ago, Wood Tavern owner and Wood Tavern Group founder Cesar Morales announced that he is shutting down both of his most recent nightlife ventures, Bizerq and Be... More >>
Break out the party favors. And hide your grandmas. 'Cause it's almost time for Fool's Gold Day Off. Last year, this annual Art Basel Miami Beach week get-down brought out 305 legend Trick Daddy an... More >>


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26 results for "aqib talib"
NFL - New England Patriots' secondary will surpass the Seattle Se...KC Joyner
There's no question the Seattle Seahawks ended the 2013 season with the league's most dominant secondary. But the New England Patriots look poised to steal that title from them in 2014.Story | Conversation | July 08, 2014
Why the Philadelphia Eagles won't miss DeSean Jackson - NFLKC Joyner
There are four main reasons why the Philadelphia Eagles won't miss leading receiver DeSean Jackson during the 2014 NFL season. KC Joyner explains.Story | Conversation | June 26, 2014
Talib's contract, Broncos' capTom Carpenter
The Denver Broncos lured former New England Patriots cornerback Aqib Talib westward with a six-year, $57 million contract on Wednesday. However, as typically is the case, those numbers are the maximums for what his deal offers. The end result likely ...Blog | March 14, 2014
2014 NFL Free Agency - Aqib Talib's contract among early deals th...Sam Monson
Sam Monson of Pro Football Focus checks in after a furious start to NFL free agency, citing five free-agent deals that don't make much sense.Story | Conversation | March 12, 2014
Are Broncos risking future to win now?Mike Sando
The Denver Broncos own the NFL's best regular-season record in the two seasons since quarterback Peyton Manning improbably hit the market and signed with them. However, they are only 2-2 in the playoffs over that span, compared to a combined 12-3 for...Blog | March 12, 2014
Redskins want Talib?Tom Carpenter
Since the New England Patriots decided against slapping the franchise tag on cornerback Aqib Talib, he's going to test the market and may well be priced out of their range. One team that appears ready to make a move on Talib is the Washington Redskin...Blog | March 09, 2014
Potential offseason DB targets for WASTom Carpenter
Only one defense in the NFL gave up more points per game than the Washington Redskins (29.9) in 2013, so it's clear that they have myriad holes to fill on that side of the ball this offseason. "Upgrading the secondary has to top the list," wrote M...Blog | January 28, 2014
Aqib Talib's knee fine?Nic Reiner
New England Patriots top corner Aqib Talib proved his worth as a shutdown cover guy this season; his absence (knee) for more than half of his team's loss in AFC Championship game last Sunday showed how much the secondary is dependent on his presence....Blog | January 22, 2014
Why Josh Gordon is NFL's best WRJohn Parolin
John Parolin of ESPN Stats & Info explains why Cleveland Browns receiver Josh Brown is the best at his position in the NFL.Story | Conversation | December 13, 2013
Talib-Gordon marquee matchupNic Reiner
Cleveland Browns wide receiver Josh Gordon has accumulated 498 receiving yards the past two weeks, the most in two consecutive games in NFL history. His 1249 yards this year is third NFL-wide, even having missed two games. He's first overall in yards...Blog | December 07, 2013
Ranking the Super Bowl contendersMike Sando
Peyton Manning went into the Denver Broncos' Sunday night game in chilly Foxborough, Mass., with a reputation for freezing in the cold. His teams had gone 4-3 over the past seven-plus seasons when the temperature at kickoff was colder than 40 degrees...Blog | November 25, 2013
NFL - Richard Sherman, Patrick Peterson among top 10 cornerbacksHerm Edwards
Herm Edwards breaks down his list of the NFL's top 10 cornerbacks, including Richard Sherman and Patrick Peterson. Darrelle Revis is on the list, but he's not No. 1 this time.Story | Conversation | November 12, 2013
NFL - Aqib Talib among pending free agents with hottest stockSteve Palazzolo
Steve Palazzolo takes a look at the five pending free agents with the hottest stock, including Aqib Talib and Jimmy Graham.Story | Conversation | November 06, 2013
NFL - Aqib Talib among free-agent signings that have provided the...Steve Palazzolo
Steve Palazzolo of Pro Football Focus explores which NFL players have provided the best value in the 2013 season compared to their price tag in free agency. The list is led by New England Patriots CB Aqib Talib.Story | Conversation | October 16, 2013
NFL - Decline could be ahead for New England PatriotsKC Joyner
KC Joyner examines the early returns out of New England and sees a number of issues that could portend a fall for the Patriots.Story | Conversation | September 20, 2013
NFL - Why Josh Freeman is holding back the Tampa Bay BuccaneersRivers McCown
Rivers McCown takes a look at the 0-2 Tampa Bay Buccaneers and why their issues start with Josh Freeman.Story | Conversation | September 17, 2013


text:
The great train-set robbery
POLICE are on the hunt for a sack full of stolen goods after gang of heartless thieves tunnelled into Moonah's Toyworld store.
Hotel rooms go boom
HOTEL operators in southern Tasmania are hailing a return to the good old days, having recorded a 90 per cent occupancy last month.
Pay-less power strategy
SMART meters, an increased uptake of electric vehicles and a second Basslink cable look set to be part of Tasmania's energy future.
Sea Shepherd activists told to lay off
ECO warrior group Sea Shepherd has been warned that staging citizen's arrests on the high seas will not help authorities convict poachers.
Christmas oyster spill spoil stinks
A SEWAGE spill into the Coal River at Richmond has delayed the harvest of about one million oysters at Pittwater.
Three Peaks race called off for 2015
A LACK of interest in next year's Three Peaks Race has forced organisers to cancel the event and announce the organisation will go into recess.
Display captures Santa's spirit
THE spirit of Christmas is alive and well at Gagebrook with Santa in residence in one garden.
Trailer firm slides into new role
TASMANIA is providing specialist engineering to keep France's snow trains in the Antarctic running.
Concerns at cycling danger spot
COMMUNITY groups will lobby the Government for safety improvements to the Channel Highway at Bonnet Hill.
Falls Festival is set to rock
EVERYTHING is falling into place for Tasmania's biggest annual music event, which begins a week from today.
Beauty treatment for ugly spuds
A MARION Bay family has created a new use for 60 tonnes of potatoes deemed not pretty enough to sell.
Christmas is a time of hope
EDITORIAL IT is considered one of the most violent events in modern history, yet even from the tragedy that was World War I there has stemmed stories of peace.
Preserving past for our future
ROBERT MORRIS-NUNN OUR state could learn a thing or two from the way the Cuban city of Havana is protecting its ancient heritage, writes Robert Morris-Nunn.
Taroona High School leavers
Taroona High School leavers' dinner at the Hotel Grand Chancellor, December 11.11
Eastside Lutheran College leavers
Eastside Lutheran College leavers' dinner at the Botanical Gardens Restaurant, December 5.7
Hailstorm hits Hobart
Hailstorm causes chaos as it sweeps across Hobart.10
Southern Christian College leavers
Southern Christian College leavers' dinner at Hobart Function Centre.5
Last children leave Christmas Island
THE last children have left detention on Christmas Island — just in time for Christmas — after a deal was secured with members of the Senate crossbench.
PM doubles women in Cabinet
TONY ABBOTT has unveiled a "significant" shake-up of his ministry, with David Johnston dumped and Sussan Ley becoming the second woman in Cabinet.
'She was saying twisted words'
NEIGHBOURS have told of what they saw Mersane Warria doing before she allegedly stabbed and suffocated to death eight children in a crime that's shocked the world.
NYPD 'cop killer's' chilling post
POLICE probe Instagram message posted hours before NYPD officers Rafael Ramos and Wenjian Liu "were simply, assassinated".
The photo banned from school yearbook
BREKKIE WRAP: WHEN this teen submitted a portrait for the high school yearbook, she wasn't prepared for the backlash.
'Islamist' killed after knife rampage
A SUSPECTED Islamist fanatic is shot dead after stabbing three police officers in a knife rampage at a police station as he shouted 'Allahu Akbar'.
The perfect Christmas ham
Chef Romeo Baudouin cooks his delicious gourmet glazed Christmas ham5:17
What is Style?
Behind the scenes with Mercury's Face of Style, Kayla Gregg.1:55
Hailstorm turns Hobart white
Hobart city turned white with hail sending Christmas shoppers rushing to the shops - for shelter.0:47
Tasmania 1940
A long lost tourist video from 1940's Tassie!4:41
Alice Springs
Gold Coast
'Canes, Sammy smash sorry Stars
THE Melbourne Stars' terrible start to BBL04 has continued, thanks to a Darren Sammy blitz and tight bowling from the Hobart Hurricanes.
Tassie talent flies flag
TASMANIAN hockey's success shows no signs of letting up with a record seven players named in national men's squads for 2015.
Shark bite proves fatal
LINDISFARNE'S Michael Hill produced one of the best innings seen in recent years in the CTPL — only to be matched in the same game.
Mighty maxi ready to rewrite history
IT will be one for the record books in the 70th Sydney to Hobart as Wild Oats XI chases an unprecedented eighth line honours title.
Johnson spearheads tough Aussie win
MITCHELL Johnson has spearheaded Australia to a 2-0 lead in the Border-Gavaskar trophy, defeating India by four wickets at the Gabba.
Keep Smith as skipper, it's his team now
STEVE Smith should remain Australia's Test captain and be appointed ODI skipper even if and when Michael Clarke does return, writes Ian Chappell.
Pain delivers big gains for beefed-up Roo
JOEL Tippett spent hours in a gym he called "The Pit" and ate six meals a day in a bid to play a key role in North Melbourne's flag assault.
Home owners awarded $340,000
A COUPLE has been awarded more than $340,000 following a court battle over their luxury Eaglehawk Neck beach house.
Preserving past for our future
OUR state could learn a thing or two from the way the Cuban city of Havana is protecting its ancient heritage, writes Robert Morris-Nunn.
Falls Festival is set to rock
EVERYTHING is falling into place for Tasmania's biggest annual music event, which begins a week from today.
Farewell from a familiar face
IT will be a sign off 30 years in the making when WIN TV's Anna McMahon finishes work tonight.
City set for wet 'n' wild ride
A SECTION of Elizabeth St in the heart of Hobart will be converted into a giant waterslide next March.
Femmes fatale still a force onstage
THEIR line-up may have changed somewhat, but the Violent Femmes are still striking a chord with listeners, writes KANE YOUNG.
Buddy pops the question to Jesinta
AFL superstar Lance 'Buddy' Franklin and model Jesinta Campbell have been flooded by well-wishes and support after announcing their engagement.
All rise, here comes Judge Gina
ALL rise for Judge Gina! Barrister and star of The Real Housewives Of Melbourne Gina Liano has filmed a TV pilot for a Judge Judy-style "people's court".
Naked Gabi Grecko roams CBD streets
CHRONIC attention-seeker Gabi Grecko may face a $590 fine after she was captured on film strutting naked through Melbourne's CBD.
Tassie's hot ticket to super summer
TASMANIA'S reputation for excellence in food, music and sporting events will rise to another level when an "epic" summer kicks off this week.
Roy's 105 and growing strong
GARDENING, surrounding yourself with family and not getting stressed are the secrets to a long life, Roy Reeves says.
Feasts without the fuss
WE'VE sought the expert advice of a handful of culinary gurus to help take some of the stress out of preparing meals this festive season.
'She died from a broken heart'
THEY married 69 years ago after a whirlwind Roman romance and never spent a day apart. This week, they died within an hour of each other.
We've done the leg work on Xmas hams
SO much ham, so little time. But not all hams are created equal. Here's our guide to how to the best around, and how to carve the perfect slice.
Australia's best mince pies revealed
FOOD critics munching on Christmas fruit mince pies have rated some of the cheapest ones as best buys. See which brands outclassed expensive boutique labels in Choice's taste test.
Christmas is a time of hope
IT is considered one of the most violent events in modern history, yet even from the tragedy that was World War I there has stemmed stories of peace.
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IN January 1984, just before he demonstrated the new Macintosh personal computer to Apple Computer Inc.'s board of directors for the first time, Steve Jobs recited a line from the Bob Dylan song ''The Times They Are A-Changin'.''
In his book ''Insanely Great,'' a history of the Macintosh computer, Steven Levy recounts the event but does not disclose which one of Dylan's lines was uttered. It is just as well, because the times they are a-changing once again, and the song is as appropriate today as it was nearly 13 years ago.
Mr. Jobs, who was ousted by Apple's board in 1985 for being too much a visionary, has now been re-hired by Apple to supply the vision (as well as the operating system software) that the company's new management desperately needs. One wonders which lyrics he sang this time.
''You better start swimmin' or you'll sink like a stone.''
It is easy sport to point to the ironies involved in Apple's $400 million purchase on Dec. 20 of Next Software Inc., the remaining half of the company Mr. Jobs started after he was expelled from Apple. He had to abandon the hardware half of Next because it was not a success.
''For the loser now will be later to win.''
Those of a more cynical nature may see Mr. Jobs's return simply as more evidence that the personal computer industry has grown old and stagnant and is recycling itself.
But those who see him as a potential savior argue that Apple has been on a slide since Mr. Jobs, who had founded Apple with Stephen Wozniak eight years earlier, was exiled. Despite a number of impressive products, including the Powerbook line of portable Macintoshes and Quicktime software, and its growth to more than $10 billion in annual revenue, Apple in the post-Jobs era has never rekindled the magic spark that led to the Macintosh.
The Macintosh operating system software (Mac OS) introduced to the consumer market a number of innovations that we take for granted today: a graphical user interface with file folders and icons, the mouse, point-and-click commands, drop-down menus, 3.5-inch diskettes, plain-English file names, multimedia, simple networking, plug-and-play peripherals and so on.
It took competitors nearly a decade to catch up to the Mac's operating system features. But viewed from a less charitable angle, the competitors did catch up. Apple squandered the incredible head start that the Mac OS had over Microsoft's DOS and Windows operating system.
''For he that gets hurt will be he who has stalled.''
Last summer, after years of setbacks and delays, Apple's latest management team finally conceded that the efforts to build a next-generation Mac OS were a disaster.
But the extent of its distress was not apparent until rumors started circulating that Apple was negotiating to acquire an operating system from Be Inc., led by a charismatic former Apple executive, Jean-Louis Gassee.
As it turned out, the charismatic former Apple executive to whom Apple eventually turned was Mr. Jobs, whose Next Step and Open Step operating systems have been highly praised, if not widely adopted, by software developers.
According to Apple, elements of the Next software environment will be incorporated into future versions of the Mac OS beginning sometime next year. It will be 1998, however, before the next-generation Mac OS is ready.
The big question, then, is whether Next software technology that goes back nearly a decade will be perceived as bold and futuristic enough to keep Mac users from defecting to Windows NT or some other competitor that might emerge by then.
''There's a battle outside and it is ragin'. It'll soon shake your (W)indows and rattle your walls.''
In the jargon of the industry, Next's software is object-oriented, multitasking, memory-protected and scalable, which means it can run lots of different programs at once on computers ranging from dinky little portables to big network servers, all without crashing the whole system when one program misbehaves, and the programs will be less likely to misbehave because they can be written as mix-and-match components that will be easy and more efficient to create and test.
''The slow one now will later be fast.''
Still, one has to question whether Next software is truly the answer to Apple's current problems. Next did not exactly make a dent in the universe, as Mr. Jobs was fond of saying about the Macintosh. It is also currently incompatible with the Macintosh. On the other hand, there is the tantalizing prospect that the new OS may work with Intel processors.
''The old road is rapidly agin'.''
For most Macintosh owners, the two key questions are, will the Next-generation Mac OS run on my current Macintosh computer? And if so, how many of my existing software applications will work with it?
Apple's chief of technology, Ellen Hancock, said the future Mac OS will be compatible with the current Power Macintoshes product line. But does that mean that anyone with an older Mac, even one of the early Power Macs based on the first Power PC chips, is out of luck? Ms. Hancock said Apple had not yet discussed whether current System 7 software would work under the new OS.
Apple's chairman, Gilbert Amelio, has promised to provide full details of the Macintosh operating system strategy on Jan. 7 at a Macintosh computer convention in San Francisco. Millions of Mac owners will be watching closely.
''The line it is drawn,'' Mr. Dylan sang. It remains to be seen if ''the curse it is cast.''
Speaking of changing Times, Mr. Dylan also wrote,
''Come writers and critics who prophesize with your pen,
''And keep your eyes wide, the chance won't come again.''
This is the last of the personal computer columns I have had the privilege to write since the year Mr. Jobs introduced the Mac.
Thanks. And remember, back up your hard disk.
Drawing (Stuart Goldenberg)


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With a name called 'Clear Pore' you'd think this product would clear your pores. Guess again, it's just a fancy gimmic to lure you into buying the product. Did not clear my pores. Left my skin dry - the kind of dry that feels like your skin is almost desperate for moisture. Would not use it again.
I mainly use this as a once or twice a week deep cleaning "mask" treatment (especially in the summer time when my skin is super oily!), OR as a face wash when I need my skin to be super deep cleaned after those hot, sweaty days. It washes off all of that make up gunk, and leaves my skin feeling fresh and the absolute cleanest it could be. I couldn't use this on a daily basis though as it would dry my skin out something terrible, but as a every now and then "super" cleanser, a nightly cleanser, or a weekly face mask it is PERFECT!
What I love most about this product is how it literally makes my blackheads disappear like nothing else. It's a good face wash to have on hand when you feel like your regular cleanser just isn't enough, and you need something a little stronger-which we ALL know sometimes we need from time to time. It definitely helps my skin with oil control.
This is the face wash that I mostly turn to in the summer time, and especially when my acne begins to flare up, it catches those break outs and nips them in the bud because it cleanses sooo deep into your pores. I can use this mask at night when I feel my skin beginning to misbehave, follow up with my typical acne treatment, and it helps A LOT!
For me, it's one of those face washes I always have on hand when my skin needs that extra extra cleansing.
1 out of 1 people found this review helpful. Did you? Yes No
More reviews by PinkEssentials
I have very oily/acne prone skin and thought this product would work for me.I didnt like this cleanser at all. It didn't do anything to control my breakouts, didn't really help control oil and lastly, it made my face itch like crazy! I am not allergic to BP, and my face never itched from using any other other product w/ BP. This just did not work well with my skin. For some reason, I thought that using this product as a mask, would help smooth out my skin's texture, but it didn't do that either. The price is fairly decent at 6.99 but of course, I would not repurchase.
1 out of 1 people found this review helpful. Did you? Yes No
I use this as my nightly cleanser. I feel really clean and fresh when I wash it off. I rarely ever have breakouts anymore (though I use a combination of products so I can't give complete credit to the pore cleanser), so I will continue to purchase. I've gone through two tubes so far and unless this suddenly becomes ineffective for me in the future this will have a permanent place in my medicine cabinet. I also use this as a mask once or twice a week. Recommended!
I really like this mask. I use it about three times a week and usually more at that time of the month. It also works well for me in the summer to control my oil. I can use as a mask for about 10 minutes in the morning and then put on my make up. I am on my second tube!!
I never really use masks all that often. I used this just hours ago. Before it was completely dry I felt my skin get tight and burn. I washed it off. And every single area I put it was bright red. I was shocked. I have no where near sensitive skin, at all! When I touch the areas I put the mask on, it feels warm, as apposed to the areas where I didn't, and they feel normal. It feels like I got sunburn. Oh well. Whatever.
This is perfect for the hormonal acne that flares up on my chin area. I use it as a wash daily during the summer on my chin area *only*, and I use it as a mask around those times when it tends to flare year round. I have used it on my nose area before when I have been sweating outside and my nose ends up looking like an oil slick, but not a lot b/c it seems to irritate the sides of my nose occasionally. I use it in combination with a SA product (the rest of my face doesn't seem to like BP--see my other review). I hope they never discontinue it!
1 out of 1 people found this review helpful. Did you? Yes No
BP products tend to work well for me where salicylic acid products fail. This seems to be a fairly gentle, yet effective product on my skin. I use it as a cleanser most often, rather than as a mask. Like another poster mentioned, if you get hormonal breakouts, use this the week before you know it typically happens! Avoids the whole need to use a mask in the first place. I would never leave this stuff on overnight, but it seems okay when I use it as an occasional cleanser.
1 out of 1 people found this review helpful. Did you? Yes No
I am a huge fan of Neutrogena products, but I am not too big on this particular product. I bought it liking the idea of the product being a 2 in 1 cleanser/mask hybrid. I got it at Target for around 5 or 6 dollars and when I got home I used it as a cleanser. The consistancy is really thick-like a mask, and things got a little messy. I get random breakouts so I expected the product to help with my acne seeing as it has 3.5% benzoyl peroxide. I tried the product as a mask, after washing off, my skin felt tight and stung a little. I will not be repurchasing this product but it is definitely not the worst product I have tried. Three lippies!!
i thought i'd give it a try cause it just has 3.5% bp. A HUGE BLUNDER! i left it on my face overnight as spot treatment on areas that needed help, and now my face is all red all over. it's itchy too. and it just worsened my zits.
don't take chances like i did.


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A pirate accessory with a twist! Beads hang from this 2-piece moustache and goatee set with adhesive backing. Synthetic fiber. Get the most realistic look with Spirit Gum adhesive and remover (not included).
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Big Cat Coffees collecting for shelter during open house
During the event, guests will be able to tour the facility, meet the Big Cat Coffees team and sample a variety of products, including treats from local businesses such as Popcorn Passion, Woodshed Roasters, Home Town Treats Ice Cream Truck and the Liberty Steam & Grille Hot Dog Truck.
Staff will be there from the New Hampshire Humane Society, the oldest operating animal shelter in the state. To view the animals available for adoption there, go to www.nhhumane.org.
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In the last few months, Bollywood has shown that it is creating more legroom for female characters to move into uncharted territories without labelling the films heroine-oriented.
"Queen' doesn't need a Women's Day release. It could release on men's day, children's day or any other day for that matter," says Vikas Bahl. Bahl's confidence is neither singular nor misplaced. In the last few weeks we have seen female characters coming out of the shadows of their male counterparts without shouting from the rooftops. We have seen that directors need not make 'heroine-oriented' "Ghar Dwar" or "Khoon Bhari Maang" to attract the female audience or mount "A Dirty Picture" to lure a voyeur in the name of celebrating freedom of womanhood. It is not even "Kahaani", where the love angle is not at the vortex of the narrative.
In "Hasee Toh Phasee", a romantic comedy, we have a scientist with proper career plans taking on the conservative family and falling in love with a handsome guy who understands her concerns and priorities. Parineeti Chopra does almost everything that we expect from the hero of a mainstream film. Also, she is no "Biwi Ho Toh Aisi" and Siddharth Malhotra is no Farooq Sheikh supporting a bigger star.
In "Highway", a dark secret wrapped with the ingredients of a mainstream Bollywood film, the protagonist Veera suffers from Stockholm Syndrome but tells her captor Mahavir clearly that she doesn't want to have kids or get married to him. In "Queen", again a light-hearted film, a middle class Delhi girl goes on her honeymoon alone. "Our mainstream films have conditioned us to a set plan but life doesn't always work according to a plan. Along the way she grapples with the idea of right and wrong that has been shoved down her throat as tradition, and in the process many boxes get opened," says Bahl, who also co-produced "Hasee Toh Phasee". The film almost breaks the age-old cliché that if a girl smiles she is as good as snared. Earlier this year, in "Dedh Ishqiya", the big reveal centred on the two women who were in a homoerotic relationship. That the director didn't come out openly and let the shadows do the talking is a matter of taste and craft, but the fact that the big boys in the film went empty-handed is an interesting twist in a Bollywood tale. Similarly, you can't ignore the presence of solid female characters in "Ram-Leela", one of the biggest hits of last year.
Of course, there is "Gulab Gang" in the offing which seems to be pandering to the need for good old melodrama, but there again, in a departure from the past, the villain is also an independent woman. Even in tripe like "Gori Tere Pyaar Mein", we have a scene where Imran Khan points to a grey strand of hair of Kareena Kapoor and she retorts that she doesn't mind it as her focus is on her job — making a bridge in a village.
"I haven't made 'Highway' to promote the cause of female characters in mainstream cinema. I just wanted Veera to live and breathe and say something relevant during the course of the film," says Imtiaz.
Like his character, who would rather be stupid than sensible, Imtiaz kept his characters rooted. "I didn't want to soft-pedal the conflict. I felt if we keep it real the situation will get more dramatic," says Imtiaz, adding he was always confident that a country which has texts like Ramayana, Mahabharat and Gita should understand the subtleties in a piece of fiction. "It depends on how well you make it. I feel that if you are bringing something unusual to the table you should be good enough to take it to the masses. I don't see cinema as a medium for selective and niche viewing." Bahl chips in, "There should not be blanket rule that this is a mainstream film so female characters will behave in this way and this is a Shyam Benegal film so they will behave differently. In Busan International Film Festival, Korean girls could relate with Rani."
Comparing the whiff of change with the set patterns of the '80s and '90s, Deepa Sahi, who was considered an exception in those days, finds the complexity in "Highway" refreshing. "What I find heartening is you no longer need a drunken husband or an exploitative boyfriend for a girl to rebel. There is an increasing space to let her pursue and struggle with her desires, and the desire need not be physical."
Mahesh Bhatt, who has tried to break the stereotypes of female characters in his avatar as a filmmaker, says, The good thing, he says, is that the ultimate aim of the female character is no longer marriage. "It is not her cinematic Kaaba anymore."
"When the girl breaks a taboo it doesn't come with a strident background sound and these films are not being marketed as heroine-oriented," he notes. "Like in 'Highway', when the girl asks why her parents didn't tell her that she needs to protect herself from the men inside her home as well, it is a rebellion but it doesn't come with a capital R," he adds. The consistency in the tone of these films suggests that for youth — and this includes actors as well — gender equality is not something aspirational but is becoming a part of everyday life. "Alia is only 21 and Veera is also of the same age," Bhatt reminds us that she is not waiting to get mature to play complex characters. Similarly, Bahl points out that Siddharth and Rajkumar (male lead in "Queen") have been able to see the bigger picture.
Many call it a multiplex phenomenon, but Bhatt feels the idea is percolating down the pyramid. "When we were shooting for 'Citylights' recently in Lower Parel, the slum women came and asked what will happen to Arohi (central character of 'Aashiqui 2'). Will she get into a relationship or not." Now, Arohi doesn't get into the Meena Kumari mould of "Sahib Biwi Aur Ghulam" to get her boyfriend out of the alcoholic mess. "She sings in a bar but that doesn't mean she has compromised on her values. She loves the boy and is ready to sacrifice her career for him but she also has the courage to call him a coward."
The audience, says Bahl, was always ready. "It is just that these characters were not considered cinema friendly." We always needed a Sita if the heroine were to play Gita. Agrees Sahi, "When we were making 'Maya Memsaab', we thought it is too offbeat an idea but when the film released we were surprised by the response."
Imtiaz, who is on a tour to gauge the response in smaller centres, claims "Highway" has already returned the money of the investors. "It gives me the creative satisfaction that you can reach out to the audience without diluting the content."
Bhatt sums up, "When I made 'Arth', it had to wait in the cans for a year and I had to deliver a hit in between to get it released, but it surprised the distributors. Our cinema is very resistant to change and it is heartening some walls are breaking."
Keywords: Women in cinema, Bollywood, Gulab Gang, women centric movies, Highway, heroine oriented films, Parineeti Chopra, Alia Bhatt


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Cowboys Stadium to host playoff final
PASADENA, Calif. (AP)
The first championship game in the College Football Playoff will be held at Cowboys Stadium.
EXCITED YET?If you think the new playoff system name is boring, check out the logos fans can vote on.
The BCS conference commissioners announced Wednesday that Arlington, Texas, edged Tampa, Fla., in the bidding to be the site of the first title game in the new playoff system.
The game will be held Jan. 12, 2015.
The final three sites for the semifinal rotation also were announced and Cowboys Stadium came up a winner again. The Cotton Bowl, which is played at the $1.2 billion home of the NFL's Dallas Cowboys, will be part of the six-bowl rotation, along with the Chick-fil-A Bowl in Atlanta and the Fiesta Bowl in Glendale, Ariz. The Holiday Bowl in San Diego also bid for a spot in the semifinal rotation, but couldn't pull the upset.
The Rose, Orange and Sugar bowls are already part of the semifinal rotation. The Rose and Sugar will host the first semifinals Jan. 1, 2015,
The next season, the Cotton and Orange bowls will host the semifinals on New Year's Eve. The semis will be played in the Fiesta and Chick-fil-A bowls after the 2016 season.
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"The card was beautiful and the changes that were suggested by the person doing the layout were just what the card needed."
"The photos we had taken of our new baby had a lilac and grey background which perfectly complimented the colours on this card (I chose the grey print). I selected rounded corners and pearl shimmer finish and it was absolutely perfect. I also selected to print 3 pictures on the back side along with a quote- love how it turned out! A beautiful card!!"
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"Received nothing but awesome comments from family and friends who received our birth announcement card! Extremely happy with the finished card, especially being able to include 3 additional photos on the back of myself, my husband, and our first born with our new addition!"


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Join Date: Mar 2011
Location: Tucson, AZ
Posts: 480
Confused on how to spend day 2 @ parks
Our trip is just 3 months away, so I have been feeding my DL appetite by coming here & playing with touring plans on Ridemax.com. Our kids are still rather little (2 girly girls ages 4 & 6). We will be using a stroller at the parks to save their little legs from total fatigue (and saving our sanity from total melt downs). Priorities for our trip are:
- At least 1 princess meet & greet
- An appt @ Bibbidi Bobbidi Boutique
- maximize mornings for rides so we dont have to wait in long lines all day
- I am the only one who wants to ride a roller coaster like Space Mountain
- See a parade
- See Aladdin 1x @ DCA
We are NOT doing:
- Any character meals its not in the budget
- Fireworks
- World of Color
- Fantasmic
- Meals at the Blue Bayou, Carthay Circle, or similar restaurants
What I am struggling with right now is whether it is really worth it for us to stand in a line for an hour to ride Radiator Springs Racers. My kids liked the Cars movies, but, well, my kids are into princesses way more. Ive read the big long thread about the wide right technique, but since well have a stroller and our kids are still young (i.e., they cant walk very fast), I am hung up on spending up to an hour or more in line first thing in the morning to go on one ride. I am tempted to skip RSR altogether and just go on Luigis & Maters rides instead and then hit up Toy Story Midway Mania and Soarin over CA.
We ** will ** have access to early entry at DCA on the Wed we are there because well be staying at the DLH. Some other things that are roaming around my head are:
- About how long does it take to walk from the DLH to DL entrances?
- If you were going to hit up DL for a 7am early entry, what time would you want to be at the entrance for security screening before 7am?
- What might DCAs park opening be the week of Memorial Day?
- Since the DCA early entry day will be day 2 of our trip, do we even bother to hit DL on that day at park opening (which may be 8am)? Or let my tired kids sleep in a little bit and do the DCA early entry (likely at 9am, if their normal park opening is @ 10am) and then hop to DL after that?
- What is check-out time usually @ the DLH? Well be visiting DL on our check-out day. Id like for us to hit DL early that day, then hubby or I go back to DLH by check-out time to check out, store our bags & such, while the other adult stays in DL with the kids. What are others experience doing this? Did this work ok for you? What would you have done differently?
My husband thinks that I am nuts but honestly, coming here is keeping me sane & its keeping me from telling my kids about the trip (we are going to surprise them they have no idea!!!).
Earning My Ears
Join Date: Feb 2013
Location: Southern California
Posts: 36
That darn RSR throws everything into a tizzy, doesn't it? My hubby keeps laughing about my constant "strategies" but I think we might skip the wide right technique & just head straight for fast passes as you stated. It might only give one chance to ride it but in the time we could spend in line for that, we could do other stuff. We were fortunate to be selected for the passholder preview before it opened, but it was broken down for about an hour & a half. I don't want to wait in the standby line & then have it break down again, so I'm thinking fp might be the way to go.
It's maybe 5-10 minute walk from DLH to the main entrances of the park. I suggest getting to the security check point around 6:30am if your EE is for 7am so you can be already lined up at the gate.
It doesn't get as much attention as RSR, but Mater's Junkyard Jamboree is a HOOT!!! The line is usually pretty small. You're going to love Carsland, though. It's breathtaking how they were able to bring Radiator Springs to life.
"I wish we never have to grow up, off to Neverland!!"
Join Date: Feb 2013
Location: Los Angeles
Posts: 75
I would definitely do RSR on your early entry day. We got to our EE (AP holders) at about 7:15 and walked right in and right to RSR. We waited for about 15 minutes. It's worth it in my eyes, even if your girls aren't big CARS fans (neither is my 8 year old daughter - but she loves the ride!). It may be the only time you won't have to wait over an hour so I would add that to my game plan if I were you. The ride is amazing for both adults and children alike, no matter what age or gender!
(mama) (daddio) (DD8) (DS3)
Join Date: Feb 2011
Posts: 174
DCA opens at the same time as DL since Carsland opened last June. So if DL at 8 then DCA at 8, not 10 as per-Carsland.
Join Date: Mar 2011
Location: Tucson, AZ
Posts: 480
Thanks for your replies! It appears that the Touring Plans website is way out of date, in that case, because their site is predicting a 10am opening time for the Wed of Memorial Day week.
I think that we might give it the good old college try that Wed to do RSR during our early entry hour.
Join Date: Feb 2011
Posts: 174
Hours aren't released yet for Memorial Day this year so most sites are using predicted times based on last year which aren't good before June's Carsland opening. Just look at all the Feb days and see that the opening times are the same for both parks.
Join Date: Mar 2009
Location: Dublin, CA
Posts: 324
Yes you will want to do RSR even with 2 girlie girls! Our world revolved around princesses 2 years ago and though they've slowly phased them out, they still embrace it at DLR. My girls absolutely LOVE RSR, that's all they can talk about for the upcoming trip. The walk from DLH to the security check in is 5-10 minutes if the kids are riding in the stroller and you're on a mission. As far as letting the kids sleep in, I say no way, that's what afternoon breaks are for, but DH would disagree on that one. We have gone to the parks on a check-out day many times. Check out is 11. So we've extended it to 12 noon to get enough done in the morning. Or if they don't extend it, we pack up and put everything with the bell desk outside and get it when it's time go.
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The New Orleans Hornets and Los Angeles Lakers are set to tip off here at the New Orleans Arena at 7 p.m.Los Angeles is coming into tonight's game fresh off a 122-105 loss to the Thunder in Oklahoma City last night. The team flew in to New Orleans late last night, and arrived at the Arena at 5:14 p.m. this afternoon.Lakers Head Coach Mike D'Antoni said arriving the night before won't have an effect on his team.
Tonight's Hornets game against the Orlando Magic at the New Orleans Arena proceeded like you might expect between two young teams with dismal records — a back-and-forth contest with long scoreless stretches by each club, sloppy turnovers, 41 combined fouls and other attendant miseries.There was a lot of everything tonight, but the Magic had a little more of it. They won, 105-102.
The New Orleans Hornets announced the signing of center-forward Henry Sims to a 10-day contract yesterday.
The Hornets have been light on big men, with Anthony Davis out for two games with a shoulder injury, and are now without reserve forward Jason Smith. Smith will undergo surgery for a torn labrum in his right shoulder and will be out for the rest of the season.
Sims is an undrafted rookie out of Georgetown. He was signed by the Knicks on a short-term contract during the preseason, but released just over a month later.
Greivis Vasquez capped a 25-point, nine-assist performance by hitting a running floater off the backboard with 12 seconds left, and the New Orleans Hornets beat the Detroit Pistons 100-95 on Friday night to snap a two-game skid.
Eric Gordon added 21 points and Ryan Anderson 19 points and 13 rebounds for the Hornets, who were short-handed in the front court with starting forward Anthony Davis sitting out his second straight game because of a sprained left shoulder.
New Orleans Hornets general manager Dell Demps says 7-foot reserve forward and center Jason Smith will miss the rest of the season because of a torn labrum in his right shoulder.
Meanwhile, the Hornets also say rookie forward Anthony Davis will be out for Friday night's game against the Detroit Pistons because of a left shoulder sprain which occurred Tuesday night against New Jersey and also sidelined him Wednesday night at Oklahoma City. Davis will be listed as day-to-day.
Russell Westbrook scored 29 points, Kevin Durant recorded his third career triple-double and the Oklahoma City Thunder finished a perfect three-game homestand by routing the short-handed New Orleans Hornets 119-74 on Wednesday night.
Durant had 18 points, 11 rebounds and 10 assists as Oklahoma City finished a dominant four-game sweep of the season series against New Orleans, which was without starters Anthony Davis (shoulder) and Eric Gordon (knee). The Thunder led by at least 29 in three of the four meetings this season,
Deron Williams scored 33 points and the Brooklyn Nets made 12 three-point shots on their way to a 101-97 victory over the New Orleans Hornets Tuesday night at the New Orleans Arena. The Hornets, who shot only 39.5 percent over the first two quarters and were down by as many as 22 points late in the second quarter, rallied in the second half of the game. New Orleans shooters knocked down 52.3 percent of their shots in the second half, and outscored their opponents 56-44 while trying to regain the advantage.
The New Orleans Hornets took a four point lead into the final minute of Friday night's game against the Dallas Mavericks at the New Orleans Arena but couldn't close, losing 104-100.The teams exchanged the lead five times in the final period, but New Orleans seemed all but destined to put it away after Ryan Anderson's 25-foot 3-pointer hit nothing but net with 55 seconds to play, putting the Hornets up 100-96 and sending the crowd into a frenzy for their home team and the free french fries.
The New Orleans Hornets kept it close, but were unable to overcome the Chicago Bulls, losing at home Tuesday night, 96-87.Chicago's Luol Deng led the victory with 20 points. He was supported by Carlos Boozer and Joakim Noah, who combined to contribute 32 points.
Hornets rookie Austin Rivers says he isn't ready to give up on the playoffs quite yet, even though New Orleans is 19-34.
While Rivers' postseason talk sounds far-fetched, the source of his optimism is evident.
Once as bad as 5-22, the Hornets head into the All-Star break having won four of five and 14 of 26, including a 99-63 demolition of Portland on Wednesday night.


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Queen Bey's Best Hair Moments view
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As someone who loves to switch up her look, Scott arrived at the VH1 Divas event in a...
As someone who loves to switch up her look, Scott arrived at the VH1 Divas event in a twisted updo with shaven sides. This low-maintenance style is a perfect fit for the on-the-go mom.
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Taylor Momsen Style Watch: Hit or Miss?by Steve Marsi at . Comments
Taylor Momsen flashed a smile - and a little bit more - on the set of Gossip Girl in New York on Tuesday. Check out the latest style from the Gossip Girl actress - from her hair down to her jewelry and clothes - and tell us your opinion(s) of her style ...
What do you think of Taylor Momsen's latest look?
Steve Marsi is the Managing Editor of TV Fanatic. Follow him on Google+ or email him here.Tags: Gossip Girl, Fashion
She is soooooo pretty.Taylor is like,MY IDOL!Lol.
WHERE CAN I GET THE DRESS AND JACKET? ITS SO HOT~ I'M thinking of having it made.
does anyone know where to get this dress and sweater?!?!???
im absolutely dying to have both!!!
Sorry not a fan of her new style, okay she might like it for her but for the character Jenny, its so wrong. For one thing in the book Jenny is sweet and not edgy and at no point did she go edgy. i know that the book is different from the show but atleast stick their personality. Taylor is already preety without make-up so go light on the make-up and clothes wise, please dont go Skimmpy!
i think that's a look of lindsay lohan.
properly a rock star.
those crunkers. man!
stop ruining your natural beauty.
i think she better stick with her age,, she is a bit overdone in the movie.
i hated jenny H, think she should put on more cloth.
her eyeliner is wayyy to thick. she shouldnt wear so much makeup, she has soo much natural beauty
Ok, are they trying to cake the makeup on Taylor so she appears older so that we don't all cringe while she makes out with a 23 year old?
Not working for me.
Whoa, Taylor! Luv her but her new look isn't the best for a 15-year-old.
Love the outfit. Hate the hair.


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Content-Type: text/plain; charset=UTF-8
The first problem of Reaper, it lacks LADSPA and LV2 support ...
On Thu, Apr 17, 2014 at 7:53 PM, Tilo Kremer wrote:
> Hello,
Content-Type: text/html; charset=UTF-8
Content-Transfer-Encoding: quoted-printable
The first problem of Reaper, it lacks LADSPA and LV2 suppo=
rt ...On Thu, Apr 17, 2014 at 7:53 PM, Tilo Kremer <firstname.lastname@example.com> wrote:
On 04/17/2014 05:34 PM, rosea grammostola wrote:
I dreamt that dream 20 years ago, and while the oss software
ecosystem has since vastly improved it still was not fitting the
bill for me. I started to use a commercial midi daw which was
included with a hardware mixer i bought, and soon after ditched most
software altogether in favour of real (DIY and off-the-shelf)
hardware. I still use pure data though.
Bitwig looks interesting and i might give it a shot again when i
won't have to code javascript to get my hardware connected.
> Reaper via Wine, is almost against my Floss & technical principles=
> finishing touch and fluent integration between tools which makes a pro=
I wasn't so lucky getting emulated audio software to run well
enough, and meanwhile would advise to not wasting time blaming, just
use what works for you :)
> from workaround to workaround. But I don't give up hope yet! :)
the hope is still there, but being a diehard open source advocate
was not very helpful to get me going musically.
my 2cts,
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