text: Fort Hays State University Hays, KS 67601 Master of Liberal Studies (MLS) with a concentration in 'Teaching and Learning' 18-hour concentration (Based upon JROTC Online and Seminar Experiences) PSY 670 Workshop in Psychology II: Educational Psychology (3 hrs) TEEL 670 Workshop in Education II: Contemporary American Education (3 hrs) TEEL 672 Readings in Education II: Classroom Management (3 hrs) TEEL 674 Institute in Education: Secondary Methods (3 hrs) TEEL 675 Seminar in Education I: Learning and the Brain (3 hrs) (Elective: choose one of the following three courses) AEP 670 Workshop in Education: Educational Leadership Development (3 hrs) (New Instr Tng) TEEL 673 Problems in Education I: Relationships in Learning (3 hrs) (ReCert Tng) AEP 675 Seminar in Education: Thinking Maps (3 hrs) 10-hour core (Remaining 4 Core Courses*) IDS 801 Introduction to Graduate Liberal Studies (1 hr) IDS 802 Ways of Knowing in Comparative Perspective (3 hrs) IDS 803 Origins and Implications of the Knowledge Society (3hrs) IDS 804 Information Literacy (3 hrs) 3-hour culminating experience IDS 820 Projects in Liberal Studies - or an acceptable alternative (3 hrs) Comprehensive Final-read page 4 (submitted online – 8 hour written response to question(s), coordinated date and time in final semester with student's Academic Advisor) Total Credits: 31 Current Cost per credit: $235.50 (effective July 1, 2011) Estimated Total Degree Tuition: $7,300 MLS contact information: Your RTG & Associates, Inc. POCs: Kenneth Denman (Navy, Air Force, USMC, plus Spouses) TEL: 719.581.9496 Harold Tucker (Army, plus Spouses) TEL: 501.859.6113 Fort Hays State University Contact: Dr. Jim Barrett Phone: 785.628.4000, ask for Dr. Jim Barrett E-mail: firstname.lastname@example.com (Blue, underlined items are hyperlinks for your ease of use.) (continue below) 1 COURSE SCHEDULE PROPOSAL for Chapter 30, VA MGIB Bill Eligible's: 1st Semester: Either 16 week or 2nd 8 week semesters PSY 670 Workshop in Psychology II: Educational Psychology (3 hrs) AEP 670 Workshop in Education: Educational Leadership Development (3 hrs) (New Instr Tng)/, OR TEEL 673 Problems in Education I: Relationships in Learning (3 hrs) (ReCert Tng) IDS 801 Introduction to Graduate Liberal Studies (1 hr) 2nd Semester: Either 16 week or 2nd 8 week semesters TEEL 675 Seminar in Education I: Learning and the Brain (3 hrs) IDS 802 Ways of Knowing in Comparative Perspective (3 hrs) (Must register through Tiger Tracks.) 3rd Semester: Either 16 week or 2nd 8 week semesters TEEL 672 Readings in Education II: Classroom Management (3 hrs) IDS 803 Origins and Implications of the Knowledge Society (3hrs) (Must register through Tiger Tracks.) 4th Semester: Either 16 week or 2nd 8 week semesters TEEL 674 Institute in Education: Secondary Methods (3 hrs) IDS 804 Information Literacy (3 hrs) (Must register through Tiger Tracks.) 5th Semester: Either 16 week or 2nd 8 week semesters TEEL 670 Workshop in Education II: Contemporary American Education (3 hrs) IDS 820 Projects in Liberal Studies - or an acceptable alternative (3 hrs) (Must register through Tiger Tracks.) Comprehensive Final Exam (open this link - online, written or oral, coordinated with student's Academic Advisor) If you click on the course title for your RTG/JROTC related courses above, you will go to its unique web page. When on that site, click on its syllabus for your academic requirement for that course. Remember, you register for your RTG/JROTC course at the RTG pre-Registration site, and the FHSU online core course at your FHSU Student Tiger Track Account. You must pay your tuition up front with MGIB Chapter 30. You receive the following payments: Fall/Spring Semesters: ¾ times stipend ($1,473.00) of approximately $1,175+ per month Summer Semesters: full-time stipend of $1,473.00 per month GI Bill benefits used to complete degree under this scenario: 14 months (18 months calendar time) Total financial stipend over 18 months approximately $20,000.00 for pure DL effort. (100% online) REMINDER: All GI Bill Veterans must submit on-line Veterans Class Schedule each semester. At Fort Hays State University, you will receive a high quality, affordable education that prepares you to address the challenges of a changing world. Rest assured that the degree you earn through the Department of Advanced Education Programs meets the standards of the Higher Learning Commission of the North Central Association of Colleges and Schools, which is FHSU's general accreditation body. (continue below) 2 INSTRUCTIONS FOR APPLICATION TO FHSU GRADUATE SCHOOL 1. ____ Complete, sign, scan and email, or fax letter of intent: RTG-FHSU MLS Letter of Intent(see page 4, below) 2. ____ Complete and submit an on-line application http://www.fhsu.edu/academic/gradschl/apply/ 3. ____ Pay the $35.00 application fee. 4. ____ Fill out your FHSU Veteran Class Schedule & appropriate VA GI Bill Application Form (see * down below) 5. ____ Provide 2 letters of recommendation, for your area of study. These are to be completed by your professors, immediate supervisors, or individuals familiar with your academic work. The letters of recommendation should be completed on letterhead stationery or the Letter of Recommendation Form and sent directly to the Graduate School. 6. ____ Your Personal Statement. http://www.fhsu.edu/academic/gradschl/Personal-Statement/ 7. ____ Provide the Graduate School with an OFFICIAL bachelor's degree transcript which indicate the completion of a bachelor's degree from an accredited institution. The bachelor's transcript must include a minimum of 60 hours of graded credit and a grade point average (GPA) of 2.5 on the last 60 hours of undergraduate credit. OFFICIAL transcripts, by definition, are transcripts sent directly from the college or university Registrar to the Graduate School at Fort Hays State University. Transcripts mailed or brought in by the applicant are not official and, therefore, are not acceptable. The Graduate School will obtain the undergraduate transcript for FHSU students. Applicants who have earned graduate degrees from other accredited institutions may also provide an OFFICIAL Graduate Degree Transcript in addition to their OFFICIAL bachelor's degree transcript, solely for this unique degree program with RTG. When the above materials have been collected for the applicant's file in the Graduate School, the departmental file will be reviewed by a departmental committee from the applicant's major area to determine admission status. This committee will follow these guidelines: Each applicant for this Master's program must evidence a minimum grade point average of 2.5 (B) on the last 60 hours of undergraduate credit. Each applicant for this degree must evidence a grade point average of 3.0 (B) or higher in all graduate work taken at the Master's level. The student must have adequate preparation in the area of the major of study. Each applicant for a Master's degree or for admission as a non-degree student must hold a bachelor's degree from a college or university of recognized standing (regional accreditation). The committee will then return the file to the Graduate School with approval or rejection of the application clearly indicated. An advisor will be appointed to assume responsibility for the student's program and direction of study. The Graduate School will notify the applicant by letter of the status of the application. The admitted student will then be given directions to contact the graduate advisor, arrange a program of study, and refer to the graduate catalog for the fulfillment of other requirements. Please submit any downloaded/printed materials to: Graduate School Fort Hays State University 600 Park Street Hays, KS 67601 *Applicants are reminded to fill out their VA Form 22-1995 or VA Form 22-1990 (1st time applicants) for Chapter 30 FHSU's VA Facility Code Number is: 11000516. Your FHSU VA POC is Ms. Dixie Balman, Tel: 1.785.628.4408. Email: firstname.lastname@example.com **Administrative Questions or Issues with FHSU requirements please contact your RTG Mentor or at FHSU: Ms. Linda Garner, Graduate School Administrative Support at FHSU Telephone Number: 1.785.628.4236 Fax Number: 1-785-628-4479 (or by scanned email – preferred delivery method) Email: email@example.com REMINDER: All GI Bill Veterans must submit on-line Veterans Class Schedule each semester. (continue below) 3 FHSU Graduate Degree Letter of Intent: Forward to: Linda Garner, MLS w/conc. In Teaching & Learning Please list baccalaureate transcript(s) to be Telephone Number: 1-785-628-4236 received by FHSU Graduate School from the Fax Number: 1-785-628-4479 (or by scanned email) following accredited school(s): Email: firstname.lastname@example.com (preferred method) ____________________________________ All Graduate Students must have filled out their online application, ____________________________________ Click this link if you still need to: ____________________________________ http://www.fhsu.edu/academic/gradschl/apply/ ____________________________________ & pay $35, one-time only, graduate student application fee. ____________________________________ (Graduate Applicants: Do Not Submit any ACE or Have Official copy of Bachelor's degree transcript mailed directly to: Military Transcripts.) Fort Hays State University Attn: Linda Garner/Graduate School 600 Park Street Hays, KS 67601 Circle Initial Semester you are requesting to enroll in: Summer – Fall – Spring plus circle: 16 week or 2nd 8 week From: Your Full Name: __________________________________________________________________ The last four digits of your Social Security Number: _____________________________________ E-mail Address: _______________________ Military Service Branch _______________________ Phone Number: (work or cell)_______________________ (home)__________________________ Note for all Graduate Degree Applicants: Follow instructions on preceding pages (1-3). I, the undersigned, hereby authorize Fort Hays State University – Graduate School to release my provisional or official FHSU MLS Degree Program Acceptance Letter and MLS Degree Plan to RTG & Associates, Inc.; 7615 E. Windwood Way, Parker, CO 80134-6384 and/or their designated representative cited below, including any updates as required. This information is released subject to the confidentiality provisions of appropriate state and federal laws and regulations, which prohibit any further disclosure of this information without the specific written consent of the person to whom it pertains, or as otherwise permitted, by such regulations. Signature: _____________________________________________ Date: ____________ FHSU POC: Please cc: firstname.lastname@example.com (Army) my results Please cc: email@example.com (Air Force/Navy/Marines/Coast Guard) my results Please bcc: firstname.lastname@example.com (All Services or any Civilian) my results. (Continue to page 5) 4 Now, after you have earned your MLS in Teaching & Learning from Fort Hays State University, continue to use your remaining benefits for the following graduate certificates: Through the (currently institutional review/approval pending), earn a: Certificate in Operational Leadership …. 18 credits …. 10 months of stipends that comes to about $14,730.00. No book purchases required. One paper per course! And, then… Continue to use your remaining benefits for a second online certificate through Empire State College, part of the State University of New York: Graduate Certificate in Veterans Services …. 12 credits …. 8 months of stipends that comes to about $11,784.00. These then should exhaust your use of your education benefits. You will want to keep in touch with your RTG mentor the entire way. This will give a no cost Masters Degree and two graduate certificates, plus net you and your family about $46,500.00 in tax-free monies. 12 of the 20 courses are due to your military requirements, and draw heavily on your experience as both an operational and educational leader. Except for the very first semester, you will never have to take more than 2 courses in any one semester, and one, if not both of the courses involve something to do with your military background/experience. Call your service MENTOR or Bob Greene! 5


text:
- Details
These batch tanks are designed for indoor applications. Perfect for batch chemical/mixer mounting systems. Both polyethylene and metal stands designed for all styles make these tanks very versatile. The Cone Bottom tanks require the use of a stand for support. All tanks feature 8" lids and one standard fitting. Gallon/liter indicators are included on one side of the tanks. Open Top tanks include bolt-on lid for access. Optional polyethylene hinged lid is available for open top tanks. Tanks are designed for the containment of chemicals with a specific gravity of 2.0 or less. Tanks are manufactured from medium-density polyethylene with U.V. inhibitors. 55 and 100 gallon tanks include 1-1/2" standard fitting, 145-325 gallon tanks include 2" standard fitting.
- 15 Degree Metal Stand
- For 145 - 325 Gallon Batch Tanks
- Specs
SKU 017211060 Model CB036-15ST Weight 100.0000 Color N/A Size N/A Width N/A In Store Only No Price $236.97
- Warranty
Rural King provides a defect or damage warranty within 30 days of receipt. All Manufacturing Return Policies Supersede Rural King's Return Policy.
Please check our Product Manual tab for further warranty information on Ace Roto Mold
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Questions on Ace Roto Mold 36" Metal Stand for 145-325 Gallon Batch Tanks CB036-15ST
- From Gregg at 11/13/14 3:45 PM
- Q: Do you make a poly stand for the 250 gal tank? Is so, what is the item number?
- Show Answer
- A: Hello,
I contacted the manufacturer in regards to your question. They do in fact sell a 36" poly stand that SKU # is: 017211054.
Hope this is helpful to you. Thank you for visiting our website and have a wonderful day.
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- From Gregg at 11/13/14 3:45 PM

subset: commoncrawl


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Thanksgiving Book Give Away Contests have started on the Ehell blog! Check it out and enter!
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I think I'd just say something to the effect of cutting back on the size. It sounds like the people on the potentially-uninvited list are not very close to your mom, so they really shouldn't be surprised if they are removed. I'd say if they were surprised or even considered your mom rude, given the relationships you described, I'd think they would need to get a little perspective...
....... family and close friends we socialize with throughout the year.......
Miss Manners used some similar words in response to someone who wanted to change her guest list for her annual Christmas party, which also had the same problems as your mom's July 4th party, OP. She said that if anyone asked about an invitation, that the hostess could reply that they were only able to invite people that they saw frequently during the year.
Do all of the guests (besides this group) bring something - appetizers, dessert, drinks? If so, what does your mother provide in addition to the location? It sounds sort of like a potluck, which (to me) changes the issue of reciprocity.
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Yellow GMC in Redwood City, CA
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Jeb Scarecrow's Pumpkin Patch
Jana Dillon
Jeb Scarecrow is determined that he can outwit the crows who have planned a big harvest celebration just before Halloween in his pumpkin patch. He secretly carves faces in some of the pumpkins and when the candles are lit at nightfall, their eerie smiles frighten the crows. Jeb's pumpkins are also a big hit with his customers. 1992, Houghton, Ages 3 to 6, $4.95. Reviewer: Marilyn Courtot
Rattlebone Rock
Sylvia Andrews
Illustrated by Jennifer Plecas
Out in the town cemetery skeletons, ghouls, witches, ghosts and other creatures start singing and playing to a rollicking rock beat. It draws the townsfolk and they all stomped their feet and joined the rollicking beat to have the best ever Halloween. Silly fun with amusing pictures. 1995, HarperCollins, Ages 3 to 7, $13.95. Reviewer: Marilyn Courtot
Hilda and the Mad Scientist
Addie Adam
Pictures by Lisa Thiesing
Hilda thrives on helping people; her motto is, "I go where I'm needed and stay until I'm not." Often, however, her help is not very helpful. When she decides to help poor Dr. Weinerstein, who is rumored to suffer from rheumatism, she ignores the townspeople who warn her that the doctor is a mad scientist who creates monsters in his laboratory. In her usual bustling way, Hilda drives the doctor crazy. He tries to scare her away by making a monster, but his plan backfires in a surprising way. 1995, Dutton, Ages 4 to 8, $14.99. Reviewer: Dr. Judy Rowen


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GardenWeb Directory, listings for: NY, NJ, CT
(Entries are listed in random order.)
Previous Page | Page: 1 ... 4 5 6 7 8 ... 16
Institute of Ecosystem Studies
Millbrook, NY
The Institute of Ecosystem Studies does research and education on local, regional and global ecosystems. Free public attractions include display gardens, tropical greenhouse and walking trails.
Shoreham Garden Club
Shoreham, NY
Object of the Shoreham Garden Club is to promote interest in gardening and flower culture, to cooperate in the protection of the environment, and to further the beautification and conservation of the natural resources of our community.
Brigantine Garden Club
Brigantine, NJ
The Brigantine Garden Club was organized in 1980, as a nonprofit organization, for the purpose of making Brigantine a more beautiful city, to exchange ideas among members, and promote and develop civic interest in gardening.
Lewisboro Garden Club
South Salem, NY
Lewisboro Garden Club was formed to promote the art of gardening, cooperate in beautification of public grounds. Interested in gardening, flowers, birds, landscaping, enjoy interesting speakers, demonstrations, tours, good friends and parties, join us.
eHome Style Store
Bronx, NY
Online Home Decor & Accessory store. With over 150 items (and growing) to choose from for any room on your home.
Old Westbury Gardens
Old Westbury, NY
Old Westbury Gardens, part of Long Island's Gold Coast, is the former home of John S. Phipps and his family. Westbury House is nestled amid 160 acres of formal gardens, landscaped grounds, woodlands, ponds and lakes. Guided tours of house and grounds daily.
Long Island Hobby Greenhouse and Indoor Pant Society
Huntington, NY
A membership organization for people interested in indoor plants; greenhouse, windowsill, or under lights.
Queens Botanical Garden
Flushing, NY
Experience 39 acres of unsurpassed beauty and tranquility along shaded and sun-drenched paths, while enjoying seasonal displays of tulips, roses, and annuals.
Wny Carnivorous Plant Club
Buffalo, NY
The Wny Carnivorous Plant (Cp) Club is a new budding organization for amateur and professional Cp gardeners.
Frederick W. Vanderbilt Garden Association
The Fwvga is a group of volunteer gardeners dedicated to restoring and maintaining the historic, formal Italian gardens of the Frederick W. Vanderbilt Historic Site. The Fwvga is celebrating its 20th anniversary in 2004.
- Multiple keywords are searched so that each word must appear in each hit (boolean "and").
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Entries are being added regularly, so be sure to bookmark this page and stop by again!
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22.4°in Newcastle
- 18°/22°
- Saturday
- 18°/25°
- Sunday
- 19°/26°
- Monday
The New South Wales Member for Lake Macquarie Greg Piper says the family of a local teenager sentenced in Bali for drug possession, is ecstatic he is likely to return home soon.
A 14-year-old Morisset Park boy was found guilty of buying drugs in Bali and yesterday an Indonesian judge sentenced him to two months in prison.
With time already spent in custody, the teenager is expected to be released next Sunday.
Mr Piper, who is a friend of the family, says the boy's parents are incredibly relieved at the lenient sentence and that their son won't go an adult prison.
"The range of possibilities they were initially looking at was quite broad considering the worst case scenario of some years being detained in an Indonesian jail system," he said.
"That option seemed to dissipate as they got closer but still you never know until the sentence is handed down. There's a lot of relief amongst the family.
Mr Piper says the boy's parents will heed a recommendation from the prosecution that the boy receive help for his drug addiction.
"My understanding is that they've been talking about what they're going to do but they're certainly not going to just come back to business as usual," he said.
"They will be obviously addressing the situation, but the first thing is that they're going to be back home as a family and I'm very happy for them."
First posted


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Gotham Promo: The Saga Beginsby Matt Richenthal at . Comments
Well, this looks totally and completely awesome.
Fox confirmed the obvious yesterday, ordering Gotham to series for its 2014-2015 schedule and then giving fans their first look at a drama that features Ben McKenzie as detective James Gordon.
Outlined as an "origin story of the great DC Comics super-villains and vigilantes," the show promises to reveal "an entirely new chapter that has never been told."
And that's exactly what we see in the following footage, as we glimpses of such characters as Donal Logue's Harvey Bullock; Jada Pinkett Smith's Fish Mooney; and Robin Lord Taylor's Oswald Cobblepot.
Go inside one man his one mission and the one iconic city at the center of it all here:
Matt Richenthal is the Editor in Chief of TV Fanatic. Follow him on Twitter and on Google+.Tags: Gotham, Promos
My last paycheck was $19863 wơrking 17 hours a week online. I've been doing this for almost two years now, as a second job, and have made over $300k using this system. It's really user friendly, tasks simple and fun. Just follow the instructions on this page and you can start making money right away.... JOBS29.C(O)M
The only thing about Gotham is that it will be the fox network; which means it will be canceled before it even gets going. I for one am not going to invest anymore of my time or energy toward Fox shows until they show me they can keep a show on air longer than a month....
OMG!!!! THIS LOOK COMPLETELY AWESOME. I am 40 yrs old and acting like a kid in the candy store.
Well this looks interesting.
I'm so stoked for this!


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Scotland look to interim coach
ESPN Staff
December 4, 2012
Scott Johnson was present at the 2015 World Cup draw for Scotland © PA Photos
Scotland are likely to appoint an interim successor to Andy Robinson for the 2013 Six Nations, according to Scottish Rugby Union chief executive Mark Dodson.
Robinson left his post in charge of the Scotland team in the aftermath of their shock 21-15 loss to Tonga on November 24. With the Six Nations just under three months away, the SRU is facing a race against time to find a permanent successor for Robinson.
"We have already received a number of applications for the job," Dodson said in The Scotsman. "We are reviewing these as well as looking at home and abroad for the best candidate to take Scotland forward to the Rugby World Cup.
"We have the Six Nations just round the corner and, given the timescales involved, we are looking at the possibility of an interim appointment as we don't want to rush into making the decision. The most important thing we can do is get someone in to take the team forward for the long term."
Scott Johnson is seemingly in pole position for the interim role as he is currently contracted to the SRU. He took up the attack coach position in the summer and has held a head coach post before - both at Wales and the United States Eagles. Ex-Glasgow Warriors coach Sean Lineen, now in a talent recruitment role with the SRU, is also a possible candidate.
"I have not had a chance to speak to the officials, but I intend to in the next week or so," Johnson said. "That will decide where I am placed in the job and if the job suits me, and if it suits them for me to take it.
"I am secure in the fact that I have come here for a couple of years and I have only just started. There are plenty of good things in Scotland and plenty of hard working rugby people and there are plenty of good players. We need to start developing a game that suits us to play."
© ESPN Sports Media Ltd.
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Stats right: the big numbers from 2014
It was another bumper year of rugby action in 2014; join us as we take a look back at some of the season's top statistics
Focus on the salary cap shambles
Salary caps, Saints, sinners and scrums - Monday Maul looks back at the key talking points from an eventful weekend


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Fax: +44-161-446-3109
Tumor Immunology
Human T cell responses to HPV 16 E2 generated with monocyte-derived dendritic cells
Article first published online: 23 SEP 2001
DOI: 10.1002/ijc.1558
Copyright © 2001 Wiley-Liss, Inc.
International Journal of Cancer
Volume 94, Issue 6, pages 807–812, 15 December 2001
Additional Information
How to Cite
Davidson, E. J., Brown, M. D., Burt, D. J., Parish, J. L., Gaston, K., Kitchener, H. C., Stacey, S. N. and Stern, P. L. (2001), Human T cell responses to HPV 16 E2 generated with monocyte-derived dendritic cells. Int. J. Cancer, 94: 807–812. doi: 10.1002/ijc.1558
Publication History
- Issue published online: 2 NOV 2001
- Article first published online: 23 SEP 2001
- Manuscript Accepted: 4 JUL 2001
- Manuscript Revised: 30 MAY 2001
- Manuscript Received: 21 MAR 2001
Funded by
- Cancer Research Campaign
- Enact Pharma PLC
- Royal Society
- Wigan Cancer Research Fund
- Cancer Research Campaign
- Abstract
- Article
- References
- Cited By
- human papillomavirus (HPV);
- E2 protein;
- dendritic cells;
- cytotoxic T lymphocytes (CTL);
- γ-interferon ELISPOT;
- primary immune responses;
- cervical intraepithelial neoplasia (CIN);
- cervical cancer;
- vaccines
Persistent infection with human papillomavirus (HPV) type 16 has been implicated in the etiology of cervical cancer. The E2 protein is required early in viral infection and therefore may serve as a useful immune target for a vaccine aimed at prevention or therapy of premalignant lesions. Dendritic cells (DC) prepared from monocytes and pulsed with bacterially produced HPV 16 E2 C-terminus protein were used to stimulate autologous T cells over several rounds of stimulation. T cells were tested for γ-interferon release by ELISPOT and for cytotoxic activity by 51chromium release assays. To generate E2-expressing target cells for cytotoxicity assays, we constructed a recombinant vaccinia virus encoding HPV 16 E2, which was used to infect autologous Epstein-Barr virus-transformed lymphoblastoid cell lines (LCL). The results show that DC pulsed with E2 C-terminus protein induce γ-interferon-releasing T cells as demonstrated by ELISPOT. Furthermore, we demonstrate E2-specific lysis of vaccinia-E2 infected autologous LCL by CD8+ cytotoxic T lymphocytes (CTL). E2-specific CTL did not lyse untreated autologous LCL or LCL infected with wild-type vaccinia and showed low levels of cytotoxicity against natural killer cell-sensitive K562 cells. In addition, T cells stimulated with DC in the absence of E2 failed to demonstrate lysis of vaccinia-E2-labeled targets. Phenotypically, CTL populations were CD3+/CD8+. These results will facilitate the study of naturally occurring T-cell responses to HPV E2 in patients with cervical intraepithelial neoplasia and the development of immunotherapeutic strategies designed to treat this and other HPV-associated diseases. © 2001 Wiley-Liss, Inc.
Persistent infection with oncogenic human papillomaviruses (HPV) such as HPV 16 is necessary for the development of invasive cervical carcinoma.1–4 Natural immunity plays an important role in the control of HPV infection,5–7 and the identification of HPV-specific immunity in patients8–11 has provided the impetus for the development of vaccines to both prevent and treat HPV-associated disease. Most therapeutic vaccines have focused on stimulating specific cell-mediated immunity to HPV 16 E6 and E7 proteins, which are expressed in all stages of HPV-related disease development and are therefore appropriate immune targets in high grade and malignant disease.12, 13
Other early viral proteins could also be immune targets for vaccines aimed at treating HPV-associated disease. HPV 16 E2 is a 42-kDa protein comprising 3 functional domains: a 200-amino acid amino-terminal (N-terminal) activation domain14 and a 100-amino acid carboxy-terminal (C-terminal) DNA-binding domain,15 joined by a flexible hinge region.16 E2 is necessary for viral replication17, 18 and for the transcriptional control of other viral proteins, including E6 and E7.19 In cervical dysplasia, E2 expression is highest in koilocytes and decreases with increasing cervical intraepithelial neoplasia (CIN) grade.20 In the cottontail rabbit papillomavirus (CRPV) model, immunization of rabbits with DNA plasmid-encoding CRPV E2 prevented the development of skin papillomas.21, 22 These studies support the use of E2 as an immunologic target in vaccines for the protection or therapy of HPV infection and CIN. Thus virus-like particles composed of the viral capsid proteins (L1 and L2) for use in prophylaxis of HPV-associated disease have been modified to include E2 and E7 proteins. The idea is that generation of cellular immunity against the early genes may induce the regression of subclinical infections that result from imperfect neutralization of virus by antibodies.23 However, E2 gene disruption is associated with invasive disease, mechanistically via deregulation of viral oncogene expression. This association could provide an escape from E2-specific immunity, although integration into the cellular genome is not always seen in higher grade lesions and carcinomas.24
Whether E2-specific cellular immune responses play a role in the natural history of HPV infection is not known. Two studies have demonstrated helper T-cell responses to HPV 16 E2 in patients with cervical neoplasia. Lehtinen et al.25 showed T-cell proliferation in response to E2 peptides in 3 of 6 women with CIN 3 or cervical carcinoma. Bontkes et al.26 investigated helper T-cell responses to the C-terminus and N-terminus of the HPV 16 E2 protein in a cohort of 74 women with CIN. Although responses to the N-terminus were nonspecific, longitudinal analysis revealed that responses to the C-terminus occurred coincident with viral clearance.26 Cytotoxic T lymphocyte (CTL) responses to HPV 16 E2 have not been extensively studied. Konya et al.27 identified an human leucocyte antigen (HLA)-A2 E2 epitope that was capable of eliciting peptide-specific CTL in normal donors. However, the peptide-based epitope approach is restricted by the necessary use of immunodominant or subdominant peptides and by the requirement for a particular HLA type.28, 29
In the present study we investigated whether HPV 16 E2-specific CTL can be induced in normal volunteers using E2 C-terminus protein-pulsed autologous dendritic cells (DC). Using the intact protein provides all potential peptide epitopes if processed and presented by efficient antigen-presenting cells (APC) like DC, which are capable of activating naïve T cells.30 A recombinant vaccinia virus encoding HPV 16 E2 was constructed and used to infect autologous Epstein-Barr virus-transformed lymphoblastoid cell lines (LCL) as targets in cytotoxicity assays and CTL were also tested by γ-interferon (γ-IFN) ELISPOT.
Production of E2 C-terminus protein
The 86-amino acid HPV 16 E2 C-terminus protein was expressed in Escherichia coli XL1-blue cells using the expression vector pKK-E2Ct and purified according to Webster et al.31 The protein was shown to be a single band by Coomassie-stained SDS-PAGE and reactive with a polyclonal rabbit anti-E2 C-terminus antibody by Western blot (data not shown).
Description of vaccinia-E208 construction
The E2 open reading frame (ORF) from HPV 16 (nucleotides 2756–3851) was amplified by PCR and cloned into vaccinia virus insertion vector p1108, which placed the ORF under the control of the 7.5K early/late vaccinia virus promoter. The expression cassette was then inserted into the TK locus of vaccinia virus strain WR by homologous recombination, followed by selection for a cotransferred gpt marker (guanine phosphoribosyltransferase). Five clones were isolated by several rounds of plaque purification and tested for successful insertion of the E2 gene into the TK locus using PCR. Expression of the E2 protein by recombinant virus was investigated by immunofluorescence microscopy of infected HeLa cells using a rabbit polyclonal antibody against the C-terminal region of the E2 protein31 at a concentration of 1:100 and a swine anti-rabbit-fluorescein isothiocyanate antibody at 1:40 (Dako). Vaccinia-E2 infected CV-1 cells were also analyzed by Western blot for E2 protein expression. Cells infected with wild-type vaccinia and cells infected with vaccinia-E2 were prepared 24 hr before analysis by SDS-PAGE to allow maximal protein expression by the cells. Cellular protein was extracted, reduced and fractionated by 10% SDS-PAGE before being transferred to nitrocellulose. Rabbit anti-E2 C-terminus polyclonal antibody was used at a concentration of 1:50 followed by development using enhanced chemiluminescence (Amersham-Pharmacia).
Isolation of peripheral blood mononuclear cells (PBMC) and culture of DC
Peripheral blood of 3 healthy donors was obtained every 7 days. Donor A was HLA A2 negative; donors B and C were HLA A2 positive. Venous blood was heparinized (6000 IU heparin/20 ml blood) and 10 ml layered onto 10 ml Lymphoprep lymphocyte separation medium (LSM, Nycomed) and centrifuged at 900g for 20 min at 20°C. PBMC were collected from the LSM interface and washed by centrifuging at 400g in PBS for 3× 10 min. PBMC at 4 × 106 cells/ml in X-VIVO 10 medium (Biowhittaker UK Ltd) supplemented with 2 mM glutamine were transferred to tissue culture plates and incubated at 37°C for 2 hr. The nonadherent peripheral blood lymphocytes (PBL) were collected and 3 ml of X-VIVO 10 supplemented with 2 mM glutamine (Life Technologies), 100 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) and 50 ng/ml interleukin-4 (IL-4; both from R & D systems) was added to each well of adherent cells and the plates incubated at 37°C in 5% CO2. Fresh medium supplemented with GM-CSF and IL-4 was added to the cultures on day 4. All T-cell stimulation was with day 7 DC. As described below, the DC were matured with lipopolysaccharide (LPS) on day 6 and E2 antigen added for effective processing at this time. Peptides do not need the latter so were added on day 7.
DC pulsing with E2 C-terminus protein
N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl sulphate (DOTAP) liposomal transfection reagent (25 μg) (Roche) and bacterially produced purified HPV 16 E2 C-terminus protein (10 μg) were mixed at room temperature in polystyrene tubes for 20 min. After this time, 3 × 105 DC in 400 μl X-VIVO 10 with 100 ng/ml GM-CSF were added to the DOTAP/protein mixture. The DC were incubated for 4 hr at 37°C, washed twice, resuspended in 1 ml X-VIVO 10 supplemented with 100 ng/ml GM-CSF, 50 ng/ml IL-4 and 10 ng/ml LPS (Sigma) and incubated at 37°C to mature the DC. After 24 hr, DC were harvested, washed twice and resuspended in 100 μl RPMI 1640 (Life Technologies).
DC pulsing with influenza matrix peptide
Day 6 DC were suspended in 1 ml X-VIVO 10 supplemented with 100 ng/ml GM-CSF, 50 ng/ml IL-4 and 10 ng/ml LPS, and matured at 37°C for 24 hr. After this time, 3 × 105 DC in 400 μl X-VIVO 10 with 100 ng/ml GM-CSF was added to 10 μg HLA A2.1 binding influenza matrix peptide 58–66, sequence GILGFVFTL (Alta Bioscience) and incubated for 4 hr at 37°C. The DC were washed twice and suspended in 100 μl RPMI.
CD4 depletion of PBL
PBL were suspended in 80 μl of ice cold Minimacs buffer (0.5% BSA, 0.005 M EDTA in PBS, filter sterilized) and 20 μl CD4+ beads (Miltenyi Biotech) per 107 cells. The cells were incubated at 4°C for 20 min, then washed with Minimacs buffer at 400g for 10 min. The Minimacs column (Miltenyi Biotech) was prepared by attaching to the magnet and washing twice with 0.5 ml Minimacs buffer. The cells were suspended in 0.5 ml buffer and added to the top of the prepared column. The CD4-depleted fraction was collected as the column flow-through. The rationale was to favor CTL responses without removing all the T-helper activity required for optimal activation of CD8 cells.
Generation of E2 CTL
Autologous CD4-depleted PBL at a concentration of 3 × 106/ml in RPMI 1640 supplemented with 10% human AB serum (Quest Biomedical), 100 μg/ml streptomycin and 100 IU/ml penicillin (Life Technologies) were added to E2 protein-loaded DC with 10 ng/ml IL-7 (R & D systems) and cultured for 7 days. Every 2 days, 1 ml of spent medium was replaced with 1 ml fresh medium supplemented with 10 ng/ml IL-7. After 7 days in culture, T cells were restimulated using fresh day 7 DC pulsed with protein/peptide according to the methods described above. Cultures were incubated overnight at 37°C before being supplemented with 10 ng/ml IL-7 and 20 IU/ml IL-2 (R & D systems). The cultures were then incubated for 7 days. Every 2 days, 1 ml spent medium was replaced with 1 ml fresh medium supplemented with 10 ng/ml IL-7 and 20 IU/ml IL-2. T cells were tested in cytotoxicity assays after 1 or several rounds of stimulation.
ELISPOT assay
CD4-depleted PBL as above were added to autologous DC (loaded or not with E2 protein/flu peptide) and cultured in RPMI + 10% AB serum for 4 or 7 days. The 7-day cultures were restimulated with either autologous PBMC or DC (with or without antigen). ELISPOT2 was performed 4 days after the first or second stimulation. The cultures were harvested, washed and seeded in 3 replicate wells at 4 doubling dilutions from 2 × 105 cells/well of a multiscreen 96-well Immobilon-P filtration plate (Millipore) coated with anti γ-interferon antibody [capture mAb (1-D1K), ELISPOT assay kit, Mabtech] and processed as per kit instructions. Initial experiments incubated PBMC at 2 × 106/ml with 10 μg/ml of flu peptide or E2 protein in the ELISPOT plates for 48 hr. Spots were counted at each cell density using a dissecting microscope, scores normalized and the mean and standard errors calculated. The number of specific events was calculated by subtracting the normalized number of spots in control wells from those in antigen-stimulated wells.
Cytotoxicity assay
Preparation of target cells.
First, 107 PBMC were suspended in 200 μl of B95-8 Epstein Barr virus stock (donated by Dr. M. Mackett, Molecular Biology Group, PICR, UK). The cells were incubated at 37°C for 1 hr, washed and resuspended in RPMI + 10% FCS, 2 mM glutamine, 100 μg/ml streptomycin and 100 IU/ml penicillin at 5 × 105/ml. Phytohaemagglutinin (Murex Diagnostics) was added at 0.5 μg/ml and cells plated out at 2 ml/well. The cultures were checked weekly and 1 ml medium/well replaced with fresh RPMI + 10% FCS as appropriate. 1 × 106 autologous LCL cells were pelleted and infected for 1 hr at 37°C with 10 pfu/cell of virus, 24 hr before the assay. Viruses used were WR (wild-type vaccinia) and vE208 (recombinant vaccinia virus encoding HPV 16 E2). After 1 hr the cells were washed 3 times and incubated overnight in RPMI + 10% FCS before labeling with 51Cr. All target cell lines were labeled with 100 μCi (20 μl) of Na251CrO4 (NEN) for 1 hr on the day of the assay, washed 3 times and placed in a 96-well plate in aliquots of 2 × 103 targets/well.
Preparation of effector cells.
T cells were washed and resuspended in RPMI + 10% AB serum to give a maximum concentration of 2 × 105 effectors/well. Serial dilutions were prepared, with each cell concentration represented in triplicate. Maximum 51Cr release was determined from supernatants of target cells that were lysed with 100 μl of PBS/2% Tween 20 and spontaneous 51Cr release was determined by adding target cells only to the wells. Plates were incubated at 37°C for 4 hr and 100 μl of the supernatant from each well transferred to Lumaplates (Packard) and left to dry overnight. The plates were read in a Top Count plate reader (Packard). Specific lysis was calculated as: (specific release − spontaneous release) ÷ (maximum release − spontaneous release) × 100. Spontaneous release was less than 20% of maximum release. Samples were considered positive when the difference in specific lysis between the recombinant vaccinia virus infected LCL and the wild-type infected LCL was more than 10% over 2 or more E:T ratios.
Phenotypic analysis of DC
DC were prepared from adherent PBMC in serum-free medium with GM-CSF and IL-4. After 6 days of culture, DC were pulsed with E2 C-terminus protein and DOTAP or with DOTAP alone for 4 hr and then matured with LPS for 24 hr. Flu peptide was added on day 7 to matured DC. The purity of the resulting DC populations was determined by flow cytometry for the cell surface markers CD1a, HLA-DR, CD80 and CD86. High proportions of the monocyte-derived DC populations were positive for CD1a (66%), HLA-DR (96%), CD80 (49%) and CD86 (61%).
E2-specific CD8+ T-cell responses: ELISPOT
PBMC were pulsed with E2 C-terminus protein and added to the ELISPOT plate at 2 × 106/ml for 48 hr. Untreated PBMC were included as a negative control and HLA A2 influenza peptide-pulsed PBMC as a positive control. A response for the influenza peptide was detected in the 2 HLA A2 -positive donors (12.5 spots/105cells for donor B; 8.5 spots/105cells for donor C), but not in the HLA A2-negative donor (0 spots/105 cells for donor A). No responses were detected for PBMC pulsed with E2 C-terminus protein for any of the donors (data not shown). Experiments were repeated 3 times for each donor with consistent results. These results reflect immunologic memory of exposure to influenza by HLA A2-positive individuals. This is because primary in vitro antigen stimulation of naïve cells does not lead to γ-IFN release. The lack of response to E2 protein may indicate a low frequency of E2-specific CTL in the peripheral blood of normal volunteers or poor processing and presentation of the whole protein to CTL by nonprofessional APC.
This phenomenon was studied further using functional monocyte-derived DC pulsed with E2 C-terminus protein, HLA A2 influenza peptide or no antigen, to stimulate autologous CD8+-enriched T cells. After 7 days, the T cells were restimulated with fresh E2 C-terminus-pulsed DC or DC alone, or with E2 C-terminus-pulsed PBMC or PBMC alone. γ-IFN ELISPOT was performed at 4 days after the first or second stimulation (Fig. 1). Experiments were repeated 3 times for each donor with consistent results.
For the HLA A2-negative donor A, 11.7 specific events/104cells were seen after the first stimulation with DC pulsed with E2 C-terminus protein compared with 4.6 specific events/104cells with HLA A2-restricted flu peptide. For HLA A2-positive donors B and C, 1.8 and 1.2 specific events/104cells, respectively, were seen with DC pulsed with E2 C-terminus protein compared with 23.5 and 7.5 specific events/104cells after 4 days' stimulation with DC pulsed with flu peptide. These results show evidence of a higher frequency of memory T cells to the flu peptide in HLA A2-matched individuals than that detected after PBMC stimulation.
Four days after a second stimulation with DC + E2 C-terminus protein, the number of specific events for donors B and C was 54.3 and 11.4 per 104cells, respectively, compared with 1.8 and 1.2 per 104cells after the first stimulation. The same increase in specific events was not seen when the restimulation at 7 days was with PBMC + E2 C-terminus (3.1 and <0 specific events/104cells for donors B and C, respectively). For donor A, the 2 stimulations with DC loaded with HPV 16 E2 C-terminus caused the number of specific events to increase 4-fold from 11.7 per 104 cells to 47.7 per 104 cells. Unlike the other donors, donor A also showed an increase in the number of specific events after DC + E2 C-terminus for 7 days followed by PBMC + E2 C-terminus for 4 days (16.4 per 104cells).
Characterization of vaccinia-E2
To generate E2-expressing target cells for cytotoxicity assays, we constructed a recombinant vaccinia virus encoding HPV 16 E2, which was used to infect autologous Epstein-Barr virus-transformed LCL (Fig. 2). Five clones of vaccinia-E2 were isolated after 3 rounds of plaque purification and tested for the successful insertion of the E2 gene by PCR (Fig. 2b). Recombinant virus was used to infect HeLa cells at 10 pfu/cell and expression of the E2 protein was confirmed by immunofluorescence microscopy, using a rabbit anti-E2 C-terminus polyclonal antibody. All 5 clones proved positive for E2 protein expression (Fig. 2c). A single clone was selected and designated vE208. vE208-infected CV-1 cells were also analyzed by Western blot for E2 protein expression. The Western blot (Fig. 2d) shows a band near the predicted molecular weight of the full-length E2 protein of 42 kDa with the rabbit anti C-terminus antibody in the CV-1 cells infected with vE208, but not in the CV-1 cells infected with WR.
E2-specific CD8+ T-cell responses: cytotoxicity assays
T-cell responses were tested by 51Cr release assays after 2, 3, 4 and 5 stimulations with autologous monocyte-derived DC pulsed with E2 C-terminus protein or with DC alone. Target cells were autologous LCL alone, autologous LCL infected with WR, autologous LCL infected with vE208 and K562 cells. Target cell lysis by T cells stimulated with DC pulsed with E2 C-terminus protein was compared with T cells stimulated with DC in the absence of E2. Assays were repeated 4 times for each donor with comparable results. Donors B and C consistently showed evidence of E2-specific lysis of vE208-infected autologous LCL (Fig. 3). E2-specific CTL did not lyse untreated autologous LCL or LCL infected with WR and showed low levels of cytotoxicity against natural killer cell-sensitive K562 cells (less than 6% lysis). Furthermore, T cells stimulated with DC in the absence of E2 failed to demonstrate lysis of vE208-labeled targets. Phenotypically, CTL populations were CD3+/CD8+ and CD4− as assessed by flow cytometry. E2-specific lysis first became apparent after 3 or 4 stimulations of T-cell cultures, depending on the donor. Donor A did not demonstrate E2-specific lysis of vE208-infected autologous LCL.
In this study, we have investigated T-cell responses to the HPV 16 E2 C-terminus in normal volunteers. Populations of functional monocyte-derived DC were generated with surface markers as previously described.33–36 They were pulsed with E2 C-terminus protein and used to stimulate autologous T cells over several rounds of stimulation and these were tested for specific activity by ELISPOT and 51Cr-release assay. ELISPOT is a rapid and sensitive assay that detects γ-IFN secretion by antigen-specific memory T cells in response to antigen exposure.32, 37, 38 Usually, the ELISPOT assay uses HLA-restricted peptides as a source of antigen, but the assay was adapted in the present study to allow the potential detection of memory and primary T-cell responses to an HPV protein antigen after it has been processed and presented by autologous APC. This approach has the obvious advantage that it more closely resembles antigen presentation in vivo and allows CTL responses to be studied regardless of an individual's tissue type.
Primary in vitro stimulation of naïve T cells can lead to IL-2 production and proliferation but not γ-IFN secretion; this occurs following re-encounter with antigen.39 Unstimulated PBMC pulsed with E2 C-terminus protein did not induce specific γ-IFN secretion by T cells in any of the donors. This finding is consistent with a frequency of memory CTL in the peripheral blood of normal volunteers below the threshold of detection. However, T-cell responses to HPV 16 E2 were demonstrated by ELISPOT after a single stimulation of T-cell cultures with protein-loaded DC for donor A, and after 2 stimulations for donors B and C. Thus in individual A there may be a memory response that is detectable after appropriate antigen presentation or a small expansion in vitro. This finding was in contrast to the 51Cr-release assays in which a minimum of 3 in vitro stimulations were required before E2-specific lysis became apparent. Donor A was female, HLA A2 negative with no history of influenza; donor B was male, HLA A2 positive with a history of 2 recent episodes of influenza; and donor C was male, HLA A2 positive with a questionable history of influenza. After a single stimulation with either PBMC or DC pulsed with flu peptide, T cells from donors B and C showed evidence of a memory response to influenza. However, the frequency detected was 5.3- and 11.3-fold more with DC stimulation than with PBMC stimulation for donors B and C, respectively. By contrast, no significant E2 responses were seen in these donors. Donor A showed some evidence of a "memory" response to E2 compared with the irrelevant test for an HLA A2 flu peptide response after the same 4-day stimulation procedure. This E2 memory response could be enhanced or boosted by a second stimulation with protein-loaded DC (47.7/104 cells) or PBMC (16.4/104cells). This result might reflect priming of naïve T cells or an expansion of the existing memory response with antigen effectively presented by the DC. A memory response to HPV 16 E2 in a healthy volunteer may be explained by previous subclinical exposure to the virus. Genital HPV infection rates in women with normal cervical cytology varies with age and the number of lifetime sexual partners, but may be as high as 25% in women in their early 20s.40 Many of these infections are transient and disappear without ever causing dysplasia.41–43
Donors B and C did not have evidence of memory T-cell responses to HPV 16 E2, but putative primary T-cell responses could be generated in vitro after 2 stimulations with E2 C-terminus protein-loaded DC and quantified by ELISPOT assays. These apparent primary T-cell responses required antigen processing and presentation by DC as PBMC appeared to be ineffective and insufficient for eliciting the response. This finding is in contrast to donor A in whom expansion of the existing memory response to E2 could be supported by DC or PBMC, although the magnitude of response was 3-fold lower in the latter case. This finding is consistent with the theory that PBMC are able to restimulate previously activated T cells but are unable to prime naïve T cells. Studies by van der Burg et al.44 have shown that after primary activation of T cells, γ-IFN release only begins after stimulation with antigen for a second time. Using overlapping peptides to HPV 16 E7 they have mapped the E7 peptide epitope responses in normal individuals and patients with CIN or cervical cancer. The exposure to the pathogen is correlated with positive short-term ELISPOT assays in about 30% of cases.44
In the present study, all 3 donors demonstrated T-cell responses to E2 by γ-IFN ELISPOT, and only 2 of the 3 showed E2-specific cytotoxicity by 51Cr-release assays. This result required a minimum of 3 stimulations so it is not possible to know whether these are primary or memory-derived effectors. It is possible that the anti-E2 T cells generated for donor A were γ-IFN-releasing but not active in CTL assays. In any case, the specific release of γ-IFN by CTL may be sufficient to promote the eradication of HPV infection even in the absence of cytotoxicity. Although little is known about natural anti-HPV immunity, cytotoxicity and the release of type 1 cytokines such as γ-IFN and IL-2 are thought to be essential for effecting viral clearance.45 Whether antigen-specific cytotoxicity or antigen-specific cytokine release plays the dominant role in viral clearance has yet to be established, but efficient anti-HPV immunity probably depends on a complex interaction of both these and other immune mechanisms.
In this report we have demonstrated that HPV 16 E2-specific CTL can be elicited in normal volunteers using E2 C-terminus protein-pulsed autologous DC. The resulting T-cell populations are active in the γ-IFN ELISPOT system after 1 or 2 stimulations in vitro, and by 51Cr-release assays after 3 or 4 stimulations for 2 of the 3 donors. The utility of this type of methodology in the study of natural immune responses to E2 in cervical neoplasia patients now needs to be investigated and compared with the overlapping peptide approaches that have been used for HPV oncogenes.44
The research of P.L.S., M.D.B., D.J.B. and S.N.S. was supported by the Cancer Research Campaign. J.L.P. was supported by a PhD studentship from the BBSRC and Enact Pharma PLC. K.G. was supported by a research grant from the Royal Society. The Joseph Starkey Fellowship from Wigan Cancer Research Fund and The Cancer Research Campaign supported E.J.D.
We thank Ms. Y. Connolly for the immunofluorescence studies and Dr. S. Dermime for expert advice.
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- 45Immunology taught by viruses. Science 1996; 271: 173–8..


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ortolanSyllabification: or·to·lan
Pronunciation: /ˈôrt(ə)lən /
(also ortolanbunting)
Definition of ortolan in English:
A small Eurasian songbird that was formerly eaten as a delicacy, the male having an olive-green head and yellow throat.
- Emberizahortulana, family Emberizidae (subfamily Emberizinae)
Example sentences
- The ortolan was so prized as a delicacy that it was almost hunted into extinction.
- Dali was quite impressed, though he said he himself only ate 'food with faces' which meant fish and ortolans.
- Like Otto's thin-crusted, griddle-cooked pizza, possibly the most controversial foodstuff to hit Gotham since Le Cirque's ortolans.
Words that rhyme with ortolanportolan
Definition of ortolan in:
- British & World English dictionary
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Word of the day raconteur
Pronunciation: ˌrakɒnˈtəː
a person who tells anecdotes skilfully and amusingly…


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Too obese for a VBAC?
Last post: I haven't had a vbac, but had 2 vaginal births and I am...
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A little miracle is on the way
Week 1 -In the beginning there was me!
Week 2 -I'm just yolking!
Week 3 -Tiny & full of potential!
Week 4 -I'm a manatee, mommy!
Week 5 -BRAINS! BRAINS!! BRAINS!!!
Week 6 -My tail's receding!
Week 7 -Goodbye tail, hello toes!
Week 8 -Fingers-n-toes OH MY!
Week 9 -FETUS AHOY!!!
Week 10 -I can poop!
Week 11 -What's that uterus-like
Week 12 -Look ma, I'm a lollipop!
Week 13 -Farewell first trimester...
Week 14 -I can dance if I want to!
Week 15 -I'm your tiny dancer
Week 16 -I'm not gas or nervous
Week 17 -Look at me grow!!!
Week 18 -Covered in wax & nowhere to slip
Week 19 -Head-to-toe I grow-n-grow!
Week 20 -Half-way to the finish line!
Week 21 -Look Mommy, I'm dreaming!
Week 22 -I'm wrinklier than a raisin!
Week 23 -Singin' in the womb!
Week 24 -Look ma, my ears can hear!
Week 25 -To turn or not to turn...
Week 26 -Eye see YOU!
Week 27 -I'm a survivor, I'm gonna
Week 28 -I'm making blood, fat & hormones!
Week 29 -I'm spleen-tastic!
Week 30 -10 more weeks mama!
Week 31 -Another day... another ounce
Week 32 -Mommy, you're HOT!
Week 33 -I'm a chunky monkey!
Week 34 -Mama, I'm movin' downtown!
Week 35 -It's getting cramped in here!
Week 36 -Almost done, mommy!
Week 37 -I can see the finish line!
Week 38 -Stuck in a holding pattern
Week 39 -I'm almost done bakin'!
Week 40 -Three, two, one... BLAST
A new star is born
Newborn -Hello, world!
Month 1 -Hold on tight!
Month 2 -Track & focus!
Month 3 -This sounds familiar!
Month 4 -Have you heard this before?
Month 5 -Chest-ups!
Month 6 -It all goes in my mouth!
Month 7 -The final stretch!
Month 8 -Put your hands up!
Month 9 -Pointing fingers!
Month 10 -Soccer player in the making!
Month 11 -Stand up for yourself!
First Year -Happy birthday to me!


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The Rapture Announce Tour
By Tom Breihan on August 10, 2011 at 4:05 p.m. EDT
New York dance-rock kings the Rapture return with their years-in-the-making new album In the Grace of Your Love September 6 in North America and a day earlier in Europe via DFA. (In Australia and New Zealand, Modular will release it.) And over the next few months, the band will bring its furious live show back to the stages of North America and Europe. Below, check out their tour dates and stream the BNM'ed single "How Deep Is Your Love?".
Check Out the Rapture's Album Cover
By Tom Breihan on June 28, 2011 at 10:05 a.m. EDT
That image you see above is the cover art for In the Grace of Your Love, the long-awaited new album from dance-punk heroes the Rapture. The young daredevil in the picture happens to be the father of Rapture frontman Luke Jenner. He is also quite possibly the only person in the history of humanity who can look socially uncomfortable while balanced on the tip of a surfboard. As superpowers go, that one is pretty random.
Listen: The Rapture: "How Deep Is Your Love?"
By Tom Breihan on June 10, 2011 at 11:55 a.m. EDT
New York dance-punk veterans the Rapture return with new album In the Grace of Your Love September 6 in the U.S. and September 5 in Europe, via DFA, and in Australia and New Zealand via Modular. Today, DFA shared the first track "How Deep Is Your Love?" as part of its White Out Sessions video series. So that means DFA has posted a clip of someone walking into a room, slapping the 12" on a turntable, and flicking on a disco ball. The camera holds steady, and we get to hear the entire seven-minute-plus disco banger, complete with glorious saxophone solo.
The Rapture Return to DFA for New Album
By Tom Breihan on May 23, 2011 at 11:40 a.m. EDT
UPDATE: We've now got the tracklist for the new album. It's below.
Now that everyone is done making jokes about the Biblical Rapture that didn't happen this past weekend, we can get back to thinking about the only Rapture that matters: the Brooklyn dance-punk crew who released the classic single "House of Jealous Lovers" and the thoroughly awesome album Echoes on James Murphy's DFA label nearly a decade ago. The Rapture and DFA parted ways soon after Echoes.
But now, the Rapture have announced on Facebook and Twitter that they will be back in the DFA fold for their new album, The Grace of Your Love. DFA will release the album on September 6 in the U.S. and September 5 in Europe. Modular will release the album in Australia and New Zealand.
Photos: FYF Fest 2010
By Pitchfork on September 6, 2010 at 7:00 p.m. EDT
Photos by Natalie Kardos; Above: Washed Out
Los Angeles' Saturday-only FYF Fest saw performances by Panda Bear, Wavves, Ariel Pink, Washed Out, Local Natives, the Rapture and many more. Photographer Natalie Kardos was there.
The Rapture Check in From the Studio
By Jesse Will on February 18, 2010 at 9:00 a.m. EST
NYC dance-punk band the Rapture are spending February camped out with producer Philippe Zdar (one half of French house duo Cassius) at Zdar's Motorbass studio in Montmartre, Paris, recording the follow-up to their 2006 album Pieces of the People We Love. After tracking the basic elements of 15 songs in Brooklyn in December, the band went continental to finish up their new record at the same spot (then unfinished) where Zdar recorded and produced Phoenix's Grammy-winning album Wolfgang Amadeus Phoenix in 2008.
Echo Chamber: The Rapture's Luke Jenner on LCD's James Murphy
By Amy Phillips on January 26, 2010 at 1:40 p.m. EST
"A while back I got lost and didn't think that just being me was good enough, looking at what James has done just being himself has brought me a lot of peace. God Bless you dude."
-- The Rapture's Luke Jenner finds the way, thanks to his old friend/collaborator James Murphy's new LCD Soundsystem website. (Via the Rapture's blog.)
Cassius's Zdar to Produce the Rapture
By Tom Breihan on November 16, 2009 at 12:20 p.m. EST
Back in June, the Rapture announced that co-frontman Mattie Safer had left the group, and the storied New York dance-punk quartet was now a trio. In the blog post announcing Safer's departure, keyboardist Gabe Andruzzi said that the group had "been on a creative tear." Pedestrian.TV reports that to harness that creative tear, they've roped in a pretty august producer: Philippe Zdar, one half of the French dance duo Cassius.
Zdar's production credits include Phoenix's Wolfgang Amadeus Phoenix and Cut Copy's Bright Like Neon Love, and he has worked with Daft Punk, Air, and Bloc Party, among others.
Mattie Safer Leaves the Rapture
By Tom Breihan on July 15, 2009 at 12:30 p.m. EDT
According to an announcement on the Rapture's blog, bassist/vocalist Mattie Safer has left the band (via the Daily Swarm). In fact, he left the band a few months ago, back in early April. "We are sad to see him go but it seems it is the best for all of us," writes keyboardist Gabe Andruzzi.
Safer shared frontman duties with guitarist/vocalist Luke Jenner. On stage, the Rapture maintained a tricky internal dynamic between Jenner's unhinged spazzout presence and Safer's tighter, more contained energy. Plus, Safer was totally the best dancer in the band.
The good news is that Rapture aren't breaking up. In that same blog post, Andruzzi reports that they've been hard at work lately. "Luke, Vito and I have been on a creative tear," he writes. "We've been working out of our little Brooklyn make shift studio, writing, demoing and jamming. I'm pretty damn excited to share the fruits of our labor with you. But its still gonna be a minute before we hit the studio for real."
It's now going on three years since the band released their last LP, the sorely underrated Pieces of the People We Love, so it's good to hear that they're still active. The band will hit Australia for a quick tour this fall.
The full text of Andruzzi's announcement is below.
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Bard Announces Enrollment Completion Of First Ever IDE-Approved Drug-Coated Balloon Clinical Trial And Initiation Of Large Continuation Registry Study
C. R. Bard, Inc. (NYSE: BCR) today announced that its Lutonix technology center completed patient enrollment into its global, multicenter LEVANT 2 randomized clinical trial ( www.levant2.com). The purpose of this pivotal IDE trial is to compare the safety and efficacy of the Lutonix® Drug Coated PTA Dilatation Catheter (DCB) to a standard angioplasty balloon for the treatment of peripheral arterial disease.
LEVANT 2 was the first drug-coated balloon IDE trial approved by the FDA. The trial randomized 476 patients with diseased femoropopliteal leg arteries at 55 centers worldwide. Randomized patients in LEVANT 2 will be followed for five years and independent laboratories will verify trial outcomes. The Lutonix® DCB was first studied in the LEVANT 1 trial, a 101-patient first-in-human multicenter randomized trial. The company expects to release two-year follow up data from LEVANT 1 later this year.
In addition, the company also announced that it has obtained FDA approval and has initiated enrollment in the LEVANT 2 Continued Access Registry study. The first subject was enrolled on June 19, 2012 in Belgium. This new study will enroll an additional 650 patients at 70 centers worldwide. Together with the randomized patient cohort, the company expects the combined studies to deliver a scientifically significant dataset for this first of its kind technology.
The co-principal investigators for the LEVANT 2 randomized clinical trial are Dr. Kenneth Rosenfield (Massachusetts General Hospital, Boston, MA) and Prof. Dierk Scheinert (Heart Center Leipzig/Park Hospital, Leipzig, Germany). Dr. Rosenfield stated that "LEVANT 2 has been carried out to a degree of academic rigor that is unprecedented in this disease state. The controlled design of this trial is one that will offer us the first-in-kind results and insights on the role of drug-coated balloons for this population. I congratulate my fellow investigators and colleagues around the world for this major accomplishment, as well as Lutonix for conducting such a model clinical trial". Professor Scheinert adds, "We have been discussing for several years the need for a definitive clinical trial to assess the true benefit of this potentially game-changing technology, and I'm proud to be part of the first team to achieve this scientifically significant milestone."
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Nothing too interesting here; this guy is my own character design, with a Jet Set Radio style. I didn't do anything fancy with it really, just coloured it and added a ghost behind. Still, I kinda like it. ^_^
James (Fan Art Portfolio) Skater | Jet Set Radio
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Mario Batali by Dansk Classic 9 in. Square Baker - ChiantiItem # HN-LNOX277
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Bake up a batch of brownies or a mini lasagna in the Mario Batali by Dansk Classic 9 in. Square Baker in Chianti. This perfectly sized kitchen essential is made of durable, even-heating cast iron with a gorgeous chianti red enamel outside and glossy white inside. It cleans like a dream, is oven-safe, and is perfect to use on any gas, electric, induction, or ceramic-top ranges. Your brownies never had it so good.


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Williams Grand Canyon Travelodge
No availability found through our partners. Please contact the business directly or check some of our recommended alternatives.
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- Continental Breakfast
- Swimming pool
- Free parking
- Pets allowed
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Travel Blogs from Williams
Day 7 - Grand Canyon, Sedona & Phoenix
So we ended up skipping Monument Valley after all, a bit of a shame as I know a couple of us did want to see it, but there is always next time I guess. We just didn't have enough time with the lack of sleep that we have all had.
Have ended up making another trip to the Grand Canyon this morning for the sunrise, it was freezing cold, I …
Day 6 - Hoover Dam, Grand Canyon & who knows
Today's post may or may not be the last post for a few days, not sure when I'll get wifi reception next. The next few days we have no idea where we are going to sleep, we are just going to take each day as it comes. We headed towards Hoover dam first thing in the morning. Left at about 9am, leaving a few hours later than planned, as well as some …
When "Wow!" was all we could say!
... A family we met at Lake Powell the other day suggested that we should keep our heads down until we got to the railing and then look up, which is essentially what we did. And wow! Words cannot describe it. It almost seems cliche to say that, but it is true. It was fabulous. Nature has been boggling my mind so far on this trip. The people climbing over the barriers to stand on the edge also boggled my mind. Crazy! We also ...
Hoover Dam and Route 66
... but we get through. Oatman is a delight, a western time warp with donkeys everywhere! We spend too much time browsing around, eating ice cream and chatting to the locals - but what a great place. It was worth the 30 miles detour (and thirty miles back) through mountain hairpins bends to get there on this old historic route 66 road.
A long 140 miles gets to our Super 8 hotel in Williams, which is on the edge ...
From Russia, With Love
... surfaces, but those were clearly labeled). It was a lot of fun to actually touch things on display, and to climb into them (not mock-ups or models, but the real thing) was something we don't get to do much in our society.
Something special that happened while we were in the "yard" of the Petting Zoo - we saw 3 F-22s and 2 F-35s take off and fly around some. We don't get to see those ...


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I'm feeling weird..I'm shy cause I met the boy I love..And the WORST thing is I lost my best friend..What's happening?
vaneloveme 13-15, F 2 Answers 2 Nov 22, 2012 in Community
You're experiencing life ...
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newtons third law..
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My brother is mentally disabled slithly and today he beats me....i m feeling shattered why all this things are happeningto me..what to do? really dont have any idea..:(whyimalone 22-25, F 4 Answers 1 Dec 9, 2011
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The inmates of Arkham Asylum have taken over Gotham's detention center for the criminally insane on April Fool's Day, demanding Batman in exchange for their hostages. Accepting their demented challenge, Batman is forced to endure the personal hells of the Joker, Scarecrow, Poison Ivy, Two-Face and many other sworn enemies in order to save the innocents and retake the prison. During his run through this absurd gauntlet, the Dark Knight's must face down both his most dangerous foes and his inner demons.This is the critically acclaimed Batman story that helped launch the U.S. careers of Grant Morrison and Dave McKean. The classic confrontation between the Dark Knight and his archnemeses, this story is well known for its psychological intensity and probing portraits of Batman and the Joker.ARKHAM ASYLUM includes groundbreaking art that influenced scores of artists by introducing a new school of storytelling and technique to comics. This special edition also includes Morrison's complete original script, annotated by Morrison and editor Karen Berger, many of his original thumbnail breakdowns, step-by-step samples of how the story and art came together and much more.
Grant Morrison has been working with DC Comics for more than twenty years, beginning with his legendary runs on the revolutionary titles ANIMAL MAN and DOOM PATROL. Since then he has written numerous best-sellers � including JLA, BATMAN andNew X-Men � as well as the critically acclaimed creator-owned series THE INVISIBLES, SEAGUY, THE FILTH, WE3 and JOE THE BARBARIAN. Morrison has also expanded the borders of the DC Universe in the award-winning pages of SEVEN SOLDIERS, ALL-STAR SUPERMAN, FINAL CRISIS and BATMAN, INC., and he is currently reinventing the Man of Steel in the all-new ACTION COMICS.
In his secret identity, Morrison is a �counterculture� spokesperson, a musician, an award-winning playwright and a chaos magician. He is also the author of theNew York Times best-seller Supergods, a groundbreaking psycho-historic mapping of the superhero as a cultural organism. He divides his time between his homes in Los Angeles and Scotland.
This is a great story if your interested in the history of Arkham Asylum. The caricature's are written well, and dark with a little perversion. The art is weird but in a fitting way for this story.
This is a story about Batman getting trapped into coming to Arkham Asylam where it has been taken over by The Joker who has set everyone free to roam around. Batman comes face to face with many of the people he is responsible for putting there. The story is alright but the artwork is like nothing else I've seen and I'm not a fan of it.
I bought this as a gift for a longtime Batman fan and avid reader. It was absolutely loved. I had an original as a teen and I just couldn't shake the imagery and story in this graphic novel. It is by far one of the best graphic novels in the Batman collection. I highly recommend it to anyone who is just starting out in their Batman novel collection, or someone who has been a longtime fan but may have let this one slip thru the cracks.
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Free Shipping On Orders Over $75
16" Outdoor Artificial Azalea Flowers: Poly Blend UV Protected
These poly blend outdoor artificial flowers are great for placing in your yard where you need color but don't wantthe hassle of live plants. Many of us live in climates that are hard to grow flowers of certain types but these artificial ones will stay in bloom all year. You get a set of 12 flower bushes that have UV protectant right in the material itself. Not all outdoor plants are created equal because these will last much longer than your typical poly blend without UV protection thus saving you more money in the long run. Sold as a set of 2 only and available in beauty color only.
Height: 16 inches (measured from the bottom of stem to highest point)
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Set of 2 - 48 inch Artificial Cedar Topiary Trees Indoor/Outdoor
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6' Double Ball Ficus Silk Tree
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|Property type
|Minimum Stay
|2 nights
2 bedroom condo in Sports Village has new carpet, new furniture, granite counter tops in the kitchen, new big screen, new beds with pillow top mattresses, internet access with DSL from QWEST. The King bed has a Tempurpedic mattress. The beds have feather and foam pillows. There is a small washer/dryer in the bathroom closet.
This is close to golf, you can walk to swimming and tennis and the unit has covered parking, across from the unit labeled D6. This is reserved parking for D6.
There is one balcony and another walkout area.
This is a great location for the family with swimming, a clubhouse, and a nice playground. You don't need to leave to enjoy a relaxing vacation.
There is an Access Card for the Family Pool and a Key for the adult lap pool. Lost Access Cards are $50 and lost keys are $25.
Trailers are allowed in a paid parking lot at the back of the complex. Trailers and boats can be parked on the street for free.
CTO at TruTek, I enjoy hiking, biking, water skiing, and golf.
Sherri Swing purchased this condo in 2011
We enjoy St George for the trips to Zions NP, swimming with the family and early and late season golf.
There are two lakes for boating within twenty minutes of the condo, great water skiing.
It's also close to some very nice golf courses in Mesquite and the occasional trip to Las Vegas for a show.
St George, UT is great place to vacation with warm weather year round. St George is surrounded by beautiful red rock mountains that can be used for hiking, mountain biking and relaxing. Three Utah state parks in the area have fishing, boating and jet-skiing. Zions National Park is located 45 minutes from the condo. Of course golf, lots and lots of golf courses in the area. If you would rather be gambling Mesquite, NV is 30 minutes away. There is something for everyone to do in St George, UT.
|Rate Period
|Weekend Night
|Monthly *
My Standard Rate
2 night minimum stay
|Please contact owner for special rates and off season discounts.
* Approximate monthly rate. Actual rate will depend on the days of the month you stay.
Payment is usually accepted in the quoted currency (USD) unless the currency and the amount is specifically agreed in advance with the owner / advertiser.