text:
Yemen's security forces have clashed with suspected members of al-Qaeda as they hunted fighters behind a deadly attack on a government intelligence compound in the southern city of Aden.
A security official told the Reuters news agency that fighting had broken out during a search of houses in the port city's Saada district following the attack last Saturday that left 11 people dead.
Gunmen wearing military fatigues attacked the police intelligence building on Saturday, killing seven security officers, three women and a seven-year old boy, and freeing several detainees being held there.
Friday's fighting ended in several arrests, the security official said, but declined to give further details.
Security alert
Yemen's interior ministry has called for a security alert in Aden to prevent arms or fighters entering the city, which is the impoverished country's economic hub.
In a statement on its website, the ministry asked for "security bodies to tighten their grip on Aden's coast and to keep it under constant watch to prevent the infilitration of any terrorist elements into the city or the smuggling of weapons".
Yemen has attracted the attention of the international communuity since an al-Qaeda inspired group claimed responsibility for a thwarted attempt to blow up an airliner over the US city of Detriot in December last year.
Analysts say Saturday's attack show a shift in al-Qaeda tactics in Yemen with its fighters turning their attention from Western targets to the country's security forces.
"Al-Qaeda now feels under great pressure in Yemen, not only from the Yemenis but also from the United States," Mustafa Alani of the Gulf Research Centre said. "Its members are suffering from a sense of uncertainty and they have become very isolated... This attack was a show of strength."
Until the December bomb-attempt, many had criticised Sanaa's counter-terrorism efforts as half-hearted. But following the attack, the US has more than doubled its military aid to Yemen and provided "technical support" to the cash-strapped government of Ali Abdullah Saleh, the president.
This has seen an escalation in counter-terrorism efforts from the Yemeni government, analysts say.
"They tried to deprive al-Qaeda from the safe haven they enjoyed. We are witnessing a major shift here," Alani said.
The day before Saturday's attack, al-Qaeda's regional branch in Yemen called on local tribesmen to take up arms against the government.
"God willing, we will set the ground on fire beneath the tyrant infidels of Ali Saleh's regime and his American collaborators," the group said in a statement.
Yemen is struggling to curb a separatist movement in the south of the country while maintaining an uneasy ceasefire with Shia Houthi rebels in the north. Experts have said that Saturday's attack is an attempt to exploit uncertainty in the country.
Content on this website is for general information purposes only.
Your comments are provided by your own free will and you take sole responsibility for any direct or indirect
liability. You hereby provide us with an irrevocable, unlimited, and global license for no consideration to
use, reuse, delete or publish comments, in accordance with Community
Rules & Guidelines and Terms and


text:
Customers Also Bought
SunPumps SDS-D-128 minor repair kit includes the diaphragm, pistons, piston screws, outer pistons, o-rings, relief valves, check valve and cable guard screws.
This same kit covers all new and old style pumps including all SolarJack SDS-D-128 series pumps.
You will also need to order an SDS pump puller (sold seperately).
This product is shipped directly from the manufacturer or distributor and will arrive separately from any other products ordered. Availability estimates are based on average lead times and can vary due to stock status and location.
Returns & Replacements
If you are not satisfied with your purchase, you may return the product within 30 days for a replacement or a refund of the product value. Products must be unused in re-sellable condition with all original packaging, paperwork and accessories.

subset: commoncrawl


text:
Academic Writing Services
Having spent four years as an undergraduate English major, and another two as a Master's student in English Literature, I have written more than my share of lengthy academic research papers. I am very familiar with issues of citation, grammar, research protocol, and proper academic prose, and I can help you develop a topic, produce text, cite sources, and edit and proofread your paper. You can achieve the results you want, whether your need is a small research paper or a voluminous dissertation.
|Home Services Writing Samples Resources Clients Resume Blog Contact
|Copyright © Zahra Ink, Incorporated. All rights reserved.


text:
Book your room at Diamond Lodge
Please enter your travel dates.
Kimberley hotel with a 24-hour front desk
Situated near the airport, in Kimberley, this hotel is close to Big Hole, Kimberley Mine Museum Village, and De Beers Diamond Oval.
Hotel Features
Diamond Lodge provides dry cleaning/laundry services, a 24-hour front desk, a safe deposit box at the front desk, and self parking (subject to charges).
Room Amenities
Flat-screen televisions include premium satellite channels. Guestrooms also feature beds with memory foam mattresses, refrigerators, and air conditioning.Information missing or incorrect? Tell us!
At your hotel
At your hotel
Diamond Lodge features dry cleaning/laundry services and a safe deposit box at the front desk. The property has a 24-hour front desk. Parking is available onsite for a surcharge.
- 24-hour front desk
- Total number of rooms - 34
- Breakfast available (surcharge)
- Dry cleaning/laundry service
- Safe-deposit box at front desk
- Self parking (surcharge)
Park your car
Self parking (surcharge)
In your room
- Air conditioning
- Premium TV channels
- Private bathroom
- Climate control
- Phone
- Refrigerator
- Coffee/tea maker
- Daily housekeeping
- Complimentary toiletries
- Hair dryer
- Bathtub or shower
- Iron/ironing board (on request)
- In-room safe
- Desk
- Blackout drapes/curtains
- Satellite TV service
- Flat-panel TV
- Memory foam mattress
Eat and drink
Buffet breakfasts are available for a surcharge and are served each morning between 7 AM and 10 AM.
The recreational activities listed below are available either on site or nearby; fees may apply.
- Golfing nearby
The fine print for Diamond Lodge
When you arrive
Check-in time starts at 12:30 PM
Check-in time ends at 5:30 AM
When you leave
Check-out time is 10 AM
How you can pay
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Only service dogs are allowed
We should mention
Up to 2 children 12 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.


text:
Coastal Cycles Bike Program
Campus Recreation is excited to provide a FREE campus bike program, in order to offer an affordable and environmentally sustainable form of transportation to students. This program provides an alternative to driving and promote campus community. Campus Recreation has a fleet of approximately 150 cruiser bikes available for rent. All bikes come with a helmet and lock.
|Coastal Cycles Policies
To rent a bike, follow these steps:
Check it out:
Visit the HTC Outdoor Center during hours of operation and present your valid CINO Card AND another form of picture ID to check out a bicycle FREE of charge. The Outdoor Center hours vary, so check here for their hours.
Take it out:
Travel across campus in style. Park and lock your bike at various campus bike racks. We strongly encourage bikes to remain on campus during the rental period.
Bring it back:
You can rent a bike up to 30 days at a time. You must return your bike to the HTC Outdoor Center on your due date so our staff is able to provide the necessary maintenance to keep the bike in the best possible condition. Late bikes result in a $5 per day fee, until it has been returned.
Do it again:
If you would like, you may renew your bike upon return on your due date. This is dependent on availability.
The Fall 2014 hours of the HTC Outdoor Center are as follows:
|Monday - Thursday
|3 pm - 8 pm
|Friday and Saturday
|10 am - 1 pm
|3 pm - 6pm
For more information, e-mail firstname.lastname@example.com or call the Outdoor Center at 843.349.5076.
Help make our community more bike friendly by filling out this brief survey!
Your feedback is important so that we can make our community safer for bicyclists and motorists alike. If you have any questions, please contact Dr. Sharon Thompson at Coastal Carolina University by email: firstname.lastname@example.com. Your help is greatly appreciated!
Quick Links
- Hours of Operation
- Group X Nov 2014 Schedule
- Group X Finals 2014 Schedule
- IMLeagues (Intramural Sports)
- CCURec Store
- Student Webmail
Coastal Weather
Light Rain


text:
Invitation To Transmode's Interim Report Presentation On 24 July
Stockholm, Sweden – 10 July 2012 Transmode Holding AB`s Interim Report for January-June 2012 will be published at 08.00 AM CET, July 24, 2012. The Company therefore invites media, investors and analysts … Read News
Review Of Acrobat.com–Adobe's Free Online Word Processor And …
cloud computing; online word processing; free microsoft word alternatives Convert your files to PDF with Adobe's powerful PDF software; Slick interface … Read Article
The Basics Of Cloud Computing
© 2011 Carnegie Mellon University. Produced for US-CERT, a government organization. 1 The Basics of Cloud Computing Alexa Huth and James Cebula … View Doc
Cloud Computing Security Considerations
CYBER SECURITY OPERATIONS CENTRE. 6/2011: INITIAL GUIDANCE. 12 April 2011. Cloud Computing Security Considerations INTRODUCTION 1. Cloud computing offers potential benefits including cost savings and improved business outcomes for Australian government agencies. … Read Content
Cloud Computing Use Cases
Cloud Computing Use Cases A white paper produced by the Cloud Computing Use Case Discussion Group Version 2.0 30 October 2009 Contributors: Dustin Amrhein, Patrick Anderson, Andrew de Andrade, Joe … Get Doc
Sponsored By: ™ CloudU is a service mark/trademark of Rackspace US, Inc. in the United States and/or other countries The Cloud is confusing… well it can be, and that's where CloudU™ comes in. CloudU is a comprehensive Cloud Computing … View This Document
Cloud computing – Wikipédia
Dans le cas du cloud computing, elle devra connecter ces postes à Internet (directement ou pas) et ainsi les exposer à un risque d'attaque ou a des Créer un livre; Télécharger comme PDF; Version imprimable … Read Article
Cloud Computing: A Brief Introduction
Cloud Computing: A Brief Introduction LAD ENTERPRIZES, INC. www.ladenterprizes.com 610-429-3122 February 20, 2010 By: David Burford, CFA, MBA, MCP … Read Document
How To Make Pages Bigger On IPads For Books : IPad Tips – YouTube
4:15 Watch Later Error iPad 3G with 7 Terabyte External Hard Drive Attached Via Cloud Computing Explained by SBARTSTV 87,095 views 4:49 Watch Later Error PDF Reader Pro for iPad / iPad 2 / iPhone / iPod Touch HD by tomzhangus 75,979 views … View Video
FAA Cloud Computing Strategy
Http://www.cio.gov/documents/25-Point-Implementation-Plan-to-Reform-Federal%20IT.pdf 3 OMB, Federal Cloud Computing Strategy, February 8, 2011, http://www.cio.gov/documents/federal-cloud-computing-strategy.pdf … Retrieve Document
Oracle Cloud Computing
2. Executive Overview. Cloud computing is a significant advancement in the delivery of information technology and services. By providing on demand access to a shared pool of computing resources in a self-service, dynamically scaled and metered manner, cloud computing offers compelling advantages … Fetch Doc
Cloud Computing & Databases
Cloud Computing & Databases How databases can meet the demands of cloud computing by Mike Hogan, CEO ScaleDB Inc. November 14, 2008 … Document Viewer
NOT ALL CLOUDS ARE CREATED EQUAL An Expedient Communications White Paper In Cloud Computing Services January, 2010 Alex Rodriguez Vice President, Systems Engineering http://www.expedient.com … Access Document
Cloud Computing Is NP-Complete
Cloud Computing is NP-Complete Working Paper, February 21, 2011 Joe Weinman1 Permalink: http://www.JoeWeinman.com/Resources/Joe_Weinman_Cloud_Computing_Is_NP-Complete.pdf … Access Doc
Explaining Cloud Computing – YouTube
This video explains cloud computing. It is produced and presented by Christopher Barnatt, author of ExplainingComputers.com, and Associate Professor of Computing and Future … View Video
Introduction To Cloud Computing Architecture
Abstract Cloud computing promises to increase the velocity with which applications are deployed, increase innovation, and lower costs, all while increasing business agility. … Fetch Here
Cloud Computing – A Management Guide
Cloud Computing Kynetix Management Guide Page: Kynetix Technology Group 2009 2 CLOUD COMPUTING – WHAT IS IT? Let's firstly start by establishing what the word "cloud" means in this context. … View Document
CLOUD COMPUTING An Overview
Abstract Resource sharing in a pure plug and play model that dramatically simplifies infrastructure planning is the promise of "cloud computing". … View Doc
Cloud Computing
13/2 The Newsletter for Information Assurance Technology Professionals Volume 13 Number 2 • Spring 2010. Establishing Trust in . Cloud Computing … Read Content
Cloud Computing
© 2010 Gartner, Inc. and/or its affiliates. All rights reserved. Gartner is a registered trademark of Gartner, Inc. or its affiliates. For more information, e-mail firstname.lastname@example.com or visit gartner.com. EUKEINCLCOOVRW012110 … Read Here
AhnLab Reports Increasing Targeted Security Threat For Government Institutions
SEOUL, South Korea–(BUSINESS WIRE)–AhnLab Reports Increasing Targeted Security Threat for Government Institutions … Read News
AhnLab Reports Increasing Targeted Security Threat For Government Institutions
AhnLab, Inc. , a leading provider of integrated security solutions today reported Increasing targeted security threat for government institutions using crafted HWP document which is designed to exploit the vulnerability. … Read News
CSA Guidance V2
Security Guidance for Critical Areas of Focus in Cloud Computing V2.1 Prepared by the Cloud Security Alliance December 2009 … Content Retrieval


text:
Morning Media Newsfeed: Stelter Gets Movie | Profane Anchor Fired | Time Drops Forbes Bid
Click here to receive Mediabistro's Morning Media Newsfeed via email.
Brian Stelter's Top of The Morning Being Developed as Lifetime Movie (TVNewser)
Brian Stelter's Top of The Morning, which chronicled the rise of Good Morning America and the fall of Today, will soon join the pantheon of Lifetime original movies including Deadly Honeymoon, Romeo Killer and, more recently, Bonnie & Clyde. Top of The Morning: Inside The Cutthroat World of Morning TV has been optioned by Lifetime for a made-for-TV movie, TVNewser has learned. Production company DiGA Vision had previously bought the rights to the book. Lifetime confirms it is in the early development stage for the film. THR / The Live Feed The buzz-worthy book was published in April 2013, and met with mixed reviews. As part of the reporting process, Stelter, who recently moved to CNN as host of Reliable Sources, spent much of his time consumed by morning news coverage, with a heavy focus on Ann Curry's short-lived stint as co-host of Today and the high-profile rivalry between Today and now top-rated Good Morning America. NY Observer It seems like a logical fit. After all, Lifetime is a network that caters to women. And women love morning television (or so we hear). Variety Lifetime's deal to adapt Top of The Morning is sure to raise some eyebrows as the cabler is 50 percent owned by Disney, parent company of ABC. The book is generally flattering to GMA and the ABC News executive team. TVNewser For the rest of the week, we'll be asking you to cast the actors you want to portray the biggest names in TV morning news.


text:
As the only public engineering school in France to prepare executive personnel, engineers, researchers etc. and promote research studies and technology transfer for the wood and natural fibres industries, the ENSTIB is the logical partner for foreign institutions willing to develop exchanges and partnerships in these fields with and within France.
Dozens of conventions with foreign universities and institutions (Europe, Africa, Canada, Chile, ...) have been signed and are active.
Participation in the European programs : TEMPUS, PHARE, Leonardo da Vinci, COFURN, SOCRATES-ERASMUS... ENSTIB is a founding member of INNOVAWOOD.
For over 25 years, the ENSTIB has been concentrating its activities on the wood industry and has trained generations of engineers and executives holding today high-ranking positions in a field enjoying a continuous and steady development. Today, the School starts focusing on an additional, new field of expertise, namely natural and renewable fibre materials, within a Campus dedicated not only to wood but also to natural fibres. This still in association with the wood industry that brought about its renown in France and abroad.
Environmental protection and the fight against climate change have become essential elements to be taken into account for the management of all activities and also a key opportunity for the wood sector. Each player will have to show proof. Wood and environment: the up and coming sector!
Nadia Boeglin
ENSTIB Engineer, Class 1992
ADEME president's advisor from 2007 to 2008


text:
That's one reason, providers say, they aren't trying to replace your desktop hard drive. But they don't need to. Increasingly, the stuff you save and share will come from other Web sites. That's why i-drive.com, for instance, offers only 50MB worth of free storage for uploading files from your desktop, but "infinite space" for Web pages captured using its page-clipping tools.
This 4.7-million-member-strong site has partnered with a number of Web sites, like About.com, ZDNet and Homestead, in order to include its Sideload icon on their pages so you can zap Web pages to your i-drive with a mouse click or two. Other i-drive tools make it easy to build shared photo albums and MP3 playlists that you can listen to without first downloading the songs to your PC.
That has made i-drive popular with students. It claims 40 percent of its cus-tomers are businesspeople who use the site for both business and recreation.
That's the lesson learned by similarly oriented Driveway, which started life as Atrieva a couple years ago trying to sell storage to the usual early adopters (enterprises). It found better acceptance among entrepreneurial "professionals"-some 6 million of whom it has since signed up through the usual co-marketing agreements with high-traffic Web sites.
"What the professional typically does," notes Larry Jones, vice president of product marketing for Driveway, "is go to the Web to make vacation travel arrangements and then store a big Powerpoint presentation to work on at home."
His words ring true. I was trolling Microsoft's MSN Money Central recently when I came across a good article on a stock I own. Instead of downloading it, I decided to sign up for Driveway; within 60 seconds, my Driveway registration was completed and the article saved. Of course, that got me only 25MB of storage, but it's a somewhat tortuous path through referrals, file sharing and three different consumer surveys to get 100MB here.
Despite its parsimony, I have to say Driveway is my favorite virtual drive because of the site's performance. That's not a blanket recommendation. The best way to find out which site is right for you is to try them. Sign-up takes five minutes and costs you nothing. You can get around site storage limits by signing up for more than one account or with more than one provider.
You don't have to give up your desktop drive, but at the very least, a virtual hard drive provides a safe place to tuck away copies of your important files and satisfy your growing appetite for Web pages.
Like this article? Get this issue right now on iPad, Nook or Kindle Fire.
This article was originally published in the October 2000 print edition of Entrepreneur with the headline: Found In Cyberspace.


text:
Auto Vue Drive-In
3000 Liberty Ridge Road,
3000 Liberty Ridge Road,Metropolis, IL 62960
No one has favorited this theater yet
The Auto Vue Drive-In was owned by Ed Clark in the early-1960's. Capacity was 400 cars.
Contributed by Ken McIntyre
Recent comments (view all 2 comments)
rivest266 on June 9, 2012 at 2:18 pm
This was at Route 45 & Rocky Branch Road.
jwmovies on September 4, 2012 at 10:10 pm
Approx. address for this drive-in was 3000 Liberty Ridge Rd, Metropolis, IL 62960.
Want to be emailed when a new comment is posted about this theater?
Just login to your account and subscribe to this theater
Just login to your account and subscribe to this theater


text:
Skip to content
Mercedes-Benz models, news & reviews
Spy shots
04 December 2007 10:39
Very good. Yes, Mercedes is working flat out to ready its new E-class range – known internally by its W212 chassis number – for its debut at the 2009 Frankfurt motor show. That's some way off, but our spies already have most of the key features of Mercedes' big saloon and estate in the bag. Prices should start around the £33,000 mark for the entry-level E250, rising to an anticipated £75,000 for the hotshoe E63 AMG. Click next for the full story…
A Mercedes that adds some shine to its tarnished E-class reputation or a risky tech-laden move? Let us know what you think by clicking the 'Add Comment' button below…
Add your comment
Sign in You must be signed in to submit a comment.
Mercedes E-Class scooped (2007)
Your comment
By submitting your comment, you agree to adhere to the CAR Magazine website
Terms and Conditions
You must be logged in to subscribe to a topic
Login or register now
RE: Mercedes E-Class scooped (2007)
its looks a pretty old designed car
05 June 2011 06:24
Upload stories, photos or videos direct to the site, or email email@example.com.
Alternatively, call 01733 468 485 (+ 44 1733 468 485)
Seen a secret new car, fabulous exotic or have news we should publish? Then get in touch now.
Terms of use |
Privacy and Cookie Policy |
Advertise with us |
Site map |
Resources |
About Us |
Contact Us |
© Copyright 2014 Bauer Media Limited - All rights reserved.
Magazine Subscriptions | Motoring Magazine Subscriptions | Car Magazine Subscriptions | Digital Magazines| Empire | FHM | LRO | MCN | Parkers | Complaints Policy


text:
"Nothing ever changes," Rush Limbaugh lamented on Thursday. Pointing to the narrative surrounding the country's spending concerns and "manufactured" crises, the talk radio host grew frustrated, saying he's no longer getting "roped in."
It is long past time to remove the plague of Lawyers that have invaded Washington to suck the blood of PATRIOTS [tax money stolen] – Let us restore our pride and force our State duly elected Legislatures to protect WEE THE PEOPLE FROM A TYRANNICAL OPPRESSIVE CENTRAL COLLECTIVE FEDERAL GOVERNMENT.
Will you take the hours of study and weeks, months and maybe years to Restore our States Rights and Powers? Will you join with others across the many States, will you fight the good fight?
The Founders – Framers – Ratifiers all knew that the Central Federal government must be small and limited [weak] and the States should be strong and unlimited . . the following is how we the people can take our Freedoms and Liberties back . . will you do it? It is long past time to act.


text:
Heavy rain, gusty winds possible as storm moves in
Updated On: Feb 09 2013 05:44:26 PM CST
Pockets of heavy rain and gusty winds are possible as a storm system moves into the metro this weekend.
KMBC meteorologist Lisa Teachman said scattered showers would begin about 7 p.m.
"A few thunderstorms will be likely, although the majority of the activity will be on the light side," Teachman said.
Another round of rain was expected to push through early Sunday morning.
"Anywhere between 6 a.m. and 7 a.m., it will be a little stronger with winds up to 50 mph," Teachman said.
The storms should push out by mid-morning Sunday. The afternoon high is expected to climb into the middle 50s.
Stay with KMBC's FirstAlert Weather Team and KMBC.com for the latest on your forecast.
Copyright 2013 by KMBC.com All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


text:
The HUC-JIR community mourns the loss of Rabbi Zalman Schachter-Shalomi, Ph.D., an important and unique Jewish teacher, thinker, and innovator in postwar America. Rabbi, Rebbe, Master Ba'al Tefillah, and teacher to generations seeking the connections between the inclusive and loving teachings of Chasidut, Jewish mystical traditions, and the contemporary search for the spirit and the holy, Rabbi Schachter-Shalomi died on July 3, 2014 at age 89.
Born in Poland and raised in Vienna, Rabbi Schachter-Shalomi's family arrived in the United States in 1941. He received his DHL from the Cincinnati campus of HUC-JIR in 1968 with a dissertation entitled, "The Encounter (Yeḥidut): A Study of Counselling in Hasidism." He taught at Temple University and Naropa University, a Buddhist-inspired Colorado institution that is now home to Schachter-Shalomi's archives.
Known as Reb Zalman and ordained by the Lubavitcher Movement, he broke with that tradition and became a Rebbe and leader not only to his immediate followers and students, but he reached beyond the Jewish world to the mystical traditions of Islam, Christianity, Buddhism, and Native American traditions. Visionary and founder of the Jewish New Age Movement and Jewish Renewal, his influence impacted all denominations as well as the academic world.
His life long journey and mission was to breathe life, beauty, and deep meaning into prayer and Torah for all seekers. His impact on Jewish life will continue to be felt for through many disciples in North America and in Israel.
Schachter-Shalomi is survived by his wife Eve Ilsen, eleven children from three previous marriages, and thousands of devoted students.
May his memory be a blessing.
Founded in 1875, Hebrew Union College-Jewish Institute of Religion is North America's first institution of higher Jewish education and the academic, spiritual, and professional leadership development center of Reform Judaism. HUC-JIR educates men and women for service to North American and world Jewry as rabbis, cantors, educators, and nonprofit management professionals, and offers graduate programs to scholars and clergy of all faiths. With centers of learning in Cincinnati, Jerusalem, Los Angeles, and New York, HUC-JIR's scholarly resources comprise the renowned Klau Library, the American Jewish Archives, research institutes and centers, and academic publications. In partnership with the Union for Reform Judaism and the Central Conference of American Rabbis, HUC-JIR sustains the Reform Movement's congregations and professional and lay leaders. HUC-JIR's campuses invite the community to cultural and educational programs illuminating Jewish heritage and fostering interfaith and multiethnic understanding. www.huc.edu


text:
You're receiving this CLE and Events Notice as a member of the Civil Litigation Section.
Having trouble viewing this email? View it in your browser.
The technologies that generate information are ubiquitous and constantly changing. While it is not required that practicing attorneys possess all the technical knowledge of a computer expert, IT director and private investigator, it would certainly be helpful in today's ever shifting digital landscape. It remains imperative for counsel to continually refine and update their knowledge of information systems with 99% of information now being electronically generated. Today's lawyer must either acquire sufficient knowledge of ESI, or associate with the requisite talent, if one is to fulfill the obligation to represent clients competently. While perfection is not required what is required is that counsel be educated regarding the data held by their client, its relative accessibility as well as the nature of the information contained.
Please join our panel discussion regarding these topics and how to avoid some common pitfalls.
John Degnan, Moderator, Briggs and Morgan
Judge Jerome Abrams, Panelist
Kathy DiGiorno, Panelist
Heidi Fessler, Panelist
Charles Ragan, Panelist
1 hour of Standard CLE credit approved
(event code: 169141)
Date: Wednesday, June 27, 2012
Time: 12:00 p.m. to 1:00 p.m.
Location: Briggs and Morgan
2200 IDS Center, 80 South Eighth Street
Minneapolis, MN 55402 [Directions/Map]
Section Members: Free
MSBA Members (But Not CivLit Section Members): $5.00
Non-MSBA Members: $10.00
Law Students: Free
Participation by web and audio is available. Please indicate this option when registering or contact Jennifer Carter.
Cancellation Policy:
Refunds will be processed for cancellation requests received in writing on or before noon on the registration deadline. Requests should be directed to the event contact person listed at the bottom of this notice. Cancellation requests received after this time are not eligible for refunds. You may send a colleague in your place if you are unable to join us and the registration deadline has passed.
Go to the CivLit Section Website
Contact: Jennifer Carter | e-mail | Phone: (612) 278-6309
600 Nicollet Mall - Suite 380 - Minneapolis MN 55402 | Phone: (612) 333-1183 Toll-Free: (800) 882-MSBA


text:
You'll love our Sphincter sounds!
Sphincter Records is an independent record label specializing in one of a kind recordings. It's the home of Classic Snatches From Europe, Live From Uranus, Live From Down Under, Under The Double Ego, Mayhem Aforethought and more.
Currently our website is being updated; if you'd like to purchasing CD's or other Sphincter merchandise while construction is underway please visit the Kinky Friedman Cigars Store. For more information contact www.LittleJewford.com or www.DiscoverUranus.com.


text:
Diverse Fluid Handling Components and Measurement Solutions
Pro Flow Dynamics stocks and supplies a wide range of automatic and manual fuel nozzles. Certain applications may require a high flow fuel nozzle, or a standard diesel nozzle. Our high quality diesel fuel nozzle selection caters to the demands of different customers and their individual needs.
Our nozzles can be used for: gasoline, diesel, bio-diesel, oil, DEF and a variety of chemicals.
Copyright 2011, Pro Flow Dynamics, LLC
Hours of Operation:
Monday - Friday
8:00 a.m. - 5:00 p.m. PST


text:
D.C. Bar Health Law Section Newsletter April 2013
- Programs and Education
- Community Outreach
- New Practitioners
- Write for the Health Law Section Newsletter
Upcoming Program—Mark Your Calendars!
Hear from the Federal Experts:
Understanding the Final HIPAA Rules
(Rescheduled Program)
During this session, panelists from key federal agencies will provide an overview of the recently–released, long–awaited HIPAA omnibus final rule, as well as discuss the implications of key provisions for the electronic health information exchange.
Tuesday, May 28, 2013
Noon to 1:30 p.m.
D.C. Bar Conference Center
1101 K Street NW
First Floor
Please note: This program will also be available as a webinar.
Look for more details and registration information soon.
Back to top
Volunteer: Join the Programs and Education Committee
The Health Law Section's Programs and Education Committee invites you to join in planning programs for our section. You will have the opportunity to dive into aspects of health law and to connect with experienced practitioners, with the goal of planning dynamic programs for you and your colleagues. The Programs and Education Committee has monthly conference calls (generally on the third Friday of the month from 9:30 a.m. to 10:00 a.m.) to plan and coordinate programs.
To volunteer, please contact Programs and Education Committee Chair Alan Arville.
Back to top
Community Outreach
There are a number of opportunities available to Health Law Section members who wish to do community outreach projects and/or pro bono work.
Develop Materials for LawHelp.org
The D.C. Bar Health Law Section is planning to help update and/or add to the content on the LawHelp.org Web page that provides information to pro se D.C. citizens on health care issues of interest to them.
If you are interested in assisting in this endeavor or if you have any questions, please contact Health Law Section Community Outreach Coordinator Steve Chiu.
Back to top
Pro Bono Opportunities—Health Care Access Project and SSI & Disability Cases
The D.C. Bar's Pro Bono Program offers various opportunities for attorneys to assist pro bono clients in health care-related cases in the following areas:
- Medical Debt Resolution (through the Health Care Access Project)
- Patient Advocate for Interactions with Medicare/Medicaid/Private Insurer (through the Health Care Access Project)
- SSI/Disability Representation
The Health Care Access Project, which, as noted above, provides representation to low–income individuals whose health care is jeopardized either because of unmanageable medical debt or because they cannot obtain pre–approval for medical treatment or medication by their private insurer or public benefits program, holds trainings throughout the year.
Back to top
New Practitioners
Are you signed up for the Health Law Section New Practitioners group? The group is growing and is sponsoring exciting programs and events, including speed-networking, volunteer events at D.C. Central Kitchen, and career panels. To get involved with the new practitioners group, please contact New Practitioners Committee Chair Steve Chiu.
Back to top
Write for the Health Law Section Newsletter
The Health Law Section newsletter is now accepting articles on health law topics for publication in the newsletter. If you have an idea for an article, you may submit it to Health Law Section Newsletter Editor Jen Cromwell. Please include a short description of the article, and, as required by the D.C. Bar, identify the attorney who will review your article before it is submitted. The Health Law Steering Committee will consider your proposal and will notify you of whether your proposal has been accepted. The Steering Committee reserves the right to reject proposals. Please note that when you submit your article for publication in the newsletter, you will be granting the D.C. Bar the nonexclusive right to publish your article.
Back to top


text:
KidKraft helps to inspire children with the power of their imagination through unique and creative heirloom-quality wooden toys and room furnishings. * Corners double as convenient seating * Mesh cover for when sandbox is not in use * Large enough that multiple children can play at once * Reinforced wooden panels prevent warping and weathering * Sturdy Construction * Dimensions: 60" L x 60" W x 8.46" H * Product weight: 41.25 lbs.
Material: Sanmu Wood
Brand: KidKraft
Origin: Imported
Dallas-based KidKraft was started by engineers in 1968 and has since grown into a worldwide industry leader with branches in the Netherlands and Asia. KidKraft produces super-fun interactive wooden toys: small-scale kitchens complete with retro mixers and pretend cookies; dry-erase boards set on painting easels; and 100-piece table towns featuring mini airports and tiny helipads. KidKraft also produces heirloom-quality children's furniture, from toddler beds to toy chests to vanities and stools.


text:
- Authoress
- Crits and Contests
- Contact
- Success Stories
- Baker's Dozen Success Stories
- General Success Stories
- Published Authors
- Secret Agent Success Stories
- Peter Adam Salomon
- Helene Dunbar
- Beth Hautala
- Monica B.W.
- Leah Petersen
- Danielle Jensen
- Tracy Holczer
- Leigh Talbert Moore
- Alice Loweecey
- Beth Hull
- Home
Thursday, February 23, 2012
First Line Grabber #12
GENRE: Contemporary Category Romance
She pressed the receiver to her ear and heard crying—terrible soul wrenching sobs.
43 comments:
- Milena said...
No. Over-the-top angst before I have invested in anyone in the novel is a turnoff. Besides, I'd prefer to have the protag's name, or something else to anchor me in the story.
- February 23, 2012 at 9:29 AM
- sue laybourn said...
No. The 'terrible soul wrenching sobs' is way over the top as far I'm concerned. 'Heard' is a filter word. I'm not curious to read more.
- February 23, 2012 at 9:33 AM
- Elizabeth said...
No. Too much emotion without a little prelude to the reason, or a set-up, to prepare the reader for it.
- February 23, 2012 at 9:34 AM
- B.E. Sanderson said...
No. 'terrible soul-wrenching sobs' at the beginning of a romance kind of turn me off.
- February 23, 2012 at 9:40 AM
- Cheyenne Hill said...
No. I'm already depressed and I haven't read beyond the first line.
- February 23, 2012 at 9:41 AM
- Tyson said...
Bit yawn-worthy, like it's been done a hundred times before. Doesn't stand out. Also, a bit over done with the terrible soul wrenching sobs part.
- February 23, 2012 at 9:42 AM
- Alessa Hinlo said...
A bit overwrought for an opening.
- February 23, 2012 at 9:47 AM
- Alice said...
yes, I'm wondering who these people are.
- February 23, 2012 at 9:48 AM
- Holly Bodger said...
This is really close but it's a little telling. It would be stronger if she pressed the receiver to her ear and heard the sobs (the crying is implied). Also, "she" should have a name.
- February 23, 2012 at 9:56 AM
No, felt cliched in a sense. I would be more intrigued if the 'she' held the phone away from her ear, that would convey her reaction to this obviously disturbing phone call.
- February 23, 2012 at 10:03 AM
- Nicole said...
No. I feel like this has been done before, and I don't get a lot of set up to differentiate it.
- February 23, 2012 at 10:10 AM
- Jessica Negron said...
There are two things that make this not work.
1. Drawn out description of her holding a phone. These are details you have to make the reader take for granted. If you just say, "She heard crying over the phone." then we are less focused on how she is holding the phone (which is pretty universal) and more focused on the crying.
2. The last part of the sentence requires more of an emotional commitment than a reader would be prepared to make at this point. Don't tell us how heart-wrenching the sobs are yet. Tell us that she's crying. Later we'll find out why she's crying, and we'll imagine the heart-wrenching sobs then.
- February 23, 2012 at 10:14 AM
- Loralie Hall said...
No. I think it needs to be shortened and less telling to have the impact you want. Since sobbing implies crying, so to get the full impact I think you could cut straight to the soul-wrenching sobs and leave out 'crying'
- February 23, 2012 at 10:14 AM
- Katherine Ernst said...
No. I second everything everyone else said. Also, soul-wrenching should be hyphenated.
- February 23, 2012 at 10:24 AM
- KimberlyFDR said...
Too much over-the-top emotion to start off with. I want to be invested in the characters, not annoyed by them. Scale back the emotions and hook us in.
- February 23, 2012 at 10:27 AM
I think the sentence is fine, but that you could use a more original way to describe the sound.
- February 23, 2012 at 10:27 AM
- Rick said...
No. Besides the fact that it's over-the-top emotion too soon, the phrase I'm more familiar with is 'heart-wrenching'. "Soul wrenching" makes me think a bit more literally than you want to, and I wonder why the other person on the line is getting hacked by a Grim Reaper or some such. (Unless she actually is, in which case yes, I'd read more.)
- February 23, 2012 at 10:52 AM
- Jean Davis said...
No. I like soul-wrenching sobs, but the rest could be tightened to: She answered the phone and heard
- February 23, 2012 at 10:53 AM
- Erin said...
No. Starting with a pronoun turns me off as a reader. I find it hard to care about a she as much as a specific person. I also think it should be crying or sobbing, they're similar in nature but very different in contexts.
- February 23, 2012 at 11:03 AM
- Julia said...
No. It doesn't quite work for me -- there's no hook here, you just throw us into the situation.
- February 23, 2012 at 11:44 AM
- A. K. Fotinos-Hoyer said...
No. Too much emotion before Ive had a chance to get invested in the characters.
- February 23, 2012 at 12:01 PM
- Yttar said...
No. I'd like to say yes, but the part after the -- pushes it into melodramatic territory and I'd be worried the rest of the book would be that way.
- February 23, 2012 at 12:05 PM
- Sara J. Henry said...
No. A bit overwritten.
- February 23, 2012 at 12:24 PM
- Chelle Sandell said...
Yes. As an avid contemporary category romance reader this sentence immediately hooks me. I expect emotion in category books and specifically read them for that reason. I don't care for books that attempt to set-up the entire storyline in the first sentence or paragraph.
- February 23, 2012 at 12:47 PM
No- while there is tension in someone crying, I don't know or care about them yet.
- February 23, 2012 at 12:49 PM
I felt it was melodramatic and over written.
You don't need to redescribe crying, so you can tighten up the text by ditching the "soul wrenching sobs" and just saying she heard sobs. "She pressed the receiver to her ear" seems oddly specific phrasing for "answered the phone."
The trouble with this kind of opening is it shoves a lot of high emotion at the reader without much grounding. It's like: "Look! An awful situation you should care about!" even though I don't have any specific beyond sobs and a phone.
What would help me invest, I think, is some idea of what the call means to the MC. Is this a random call she picked up at home or work? Did she just dial her sister and this is the answer she got? Is she a 911 dispatcher?
To apply meaning to this scenario, I need additional context.
- February 23, 2012 at 1:15 PM
- Jenny Kaczorowski said...
It's too much all at once. It felt like jumping into cold water. And it's hard to keep that up for a whole book (and exhausting for readers), so you're starting at a high place and then either the tension will drop off or you'll keep it that high and it will loose impact. Start a little slower and you'll have more room to grow - and take your readers with you. We want to care about why someone is sobbing - or having to listen to someone sob.
- February 23, 2012 at 1:34 PM
- Larissa said...
No. I need a name, for one thing. Also, without knowing the mc or whether or not the person sobbing is close to her, I just don't have enough info to care yet. Sorry.
- February 23, 2012 at 2:33 PM
- sarahwedgbrow said...
No. It would depend on my mood whether I'd want to jump straight into this emotion.
- February 23, 2012 at 3:03 PM
- A.C. Turcotte said...
No. I'm not a fan of the terrible soul wrenching sobs. It just makes me feel like the book might be overwritten, which is not something I hugely enjoy.
- February 23, 2012 at 4:48 PM
- Cheryl said...
No. This is a lot of emotion to be faced with before I know anything about what's going on.
- February 23, 2012 at 5:07 PM
- JL Dannor said...
No. We don't know anyone here yet. Therefore, knowing someone is doing terrible soul-wrenching sobbing only makes me want your mc to get off the phone.
- February 23, 2012 at 5:10 PM
- AG said...
No, it was too melodramatic.
- February 23, 2012 at 6:34 PM
- Wen Baragrey said...
No. A bit too over the top. If someone is ringing with bad news, they're usually quiet or maybe sniffly. If they're in the middle of being murdered, they might be crying that way, but otherwise, it feels too much.
- February 23, 2012 at 8:05 PM
No, it feels too emo/melodramatic for me.
- February 24, 2012 at 1:06 AM
- Ailsa said...
No. It's not very interesting. People cry all the time - why is this significant?
- February 24, 2012 at 6:33 AM
- Shakier Anthem said...
No. Without specifics about character or situation, I'm not invested enough yet to care about this level of emotion from the characters.
- February 24, 2012 at 1:02 PM
- Nata ArtistaDonna said...
No. I have no idea what's going on. Confused.
- February 24, 2012 at 1:09 PM
- macaronipants said...
No. Doesn't grab me. I don't know who's crying, so I'm not curious as to why.
- February 24, 2012 at 2:38 PM
- A Little Push said...
No. I agree the "terrible soul wrenching sobs" is too much for the first sentence.
- February 24, 2012 at 4:46 PM
- Stephsco said...
No, I'm sorry. I think this could be tweaked to work, though. I would like to see the character's name in the first line.
- February 24, 2012 at 4:47 PM
- Traci Van Wagoner said...
No, too much for a first sentence, especially the soul wrenching sobs. That's over the top without a reason yet to care. I may have said yes without the second part.
- February 25, 2012 at 2:36 PM
As others have said, too melodramatic. And is there any reason not to give her name in the first sentence?
- February 25, 2012 at 2:46 PM


text:
Since launch mightyvites.com has sent email through Gmail using very basic HTML/ERB templates. There have been no graphics, little styling, and zero email client testing. In other words our emails have sucked and looked very unprofessional. Why? Because those are the last things you worry about when you're trying to push out the door a product that you're not sure anyone will buy. Once your site has users, however, these are the bells and whistles you need to put time into.
Recently the time came for me to spruce up the emails mightyvites.com sends. I wanted slick-looking HTML that looks good in all email clients, makes it to users' inboxes, is multipart, and can be tracked. In addition the email templates should be easy for non-geeks to edit. That way they can be changed often and not always by me.
I'm a fan of MailChimp. We use their (robust) API to programmatically manage our newsletter subscriber list. They make it very easy to compose, send, and track well-designed HTML email. They do not, however, make it easy to send transactional email. That's not their niche so they don't cater to it 1. There is a work around, but it leaves much to be desired. MailChimp transactional emails must include unsubscribe links and other CAN-SPAM compliance content which is just out of place on notices like forgotten password emails. The MailChimp email composer, however, is pretty sweet. It's easy to use and generates cross-client compatible HTML email complete with text versions for multi-part. The composer was perfect for my needs, but sending through MailChimp was no good because doing so automatically adds the unwanted mailing list links. Then it hit me: if I could compose and save my emails in MailChimp and later export them I could include the export in my Rails app and send through the email provider of my choice. Possible? With hominid, the MailChimp API Ruby gem, yes.
Hominid wraps the well documented MailChimp API for use in Ruby applications. It adds the syntactic sugar most Rubyists would expect and pretty much does a 1 to 1 mapping of the methods MailChimp defines. Part of the API deals with what MailChimp calls templates. Templates are reusable emails that you create at mailchimp.com and save to your account. You can access these HTML templates through the API and run them through a method to generate a text version. With both a HTML and text version "in hand" you can write the output to disk and have what you need for ActionMailer to generate fancy, multi-part emails that were edited online with MailChimp's slick editor. I keep all this action in a Rake task which I run every time I update a template at MailChimp.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44
desc "Task to pull customer email templates from MailChimp and cache them in the app" task :import_email_templates => :environment do # Maintain a map of ActionMailer methods to MailChimp # template names in a YAML file. YAML looks like: # <mailer class name>: # <mailer method name>: <name of MailChimp template used by method> # Example: # user_mailer: # signed_up: "Welcome To Mightyvites" # ... map=YAML.load_file(MAILER_TEMPLATE_YAML) api=Hominid::API.new(MAILCHIMP_API_KEY) # loop through the list of known MailChimp template names api.templates['user'].each do |template| html_cache=nil # find the mailer class and method this template belongs to map.each do |mailer, emails| template_name=emails.invert[template['name']] if template_name html_cache=File.join(Rails.application.config.view_path, mailer.to_s, "#{template_name}.html.erb") break end end next unless File.exist?(html_cache) # pull the latest HTML templates from MailChimp html=api.template_info(template['id'].to_i)['source'] # create a text version of the HTML for multi-part mail text=api.generate_text('html', html) # inline all CSS to ensure greatest email client compatibility html=api.inline_css(html) # overwrite mailer views File.open(html_cache, 'w+') {|f| f << html } text_cache=html_cache.gsub('.html.erb', '.text.erb') File.open(text_cache, 'w+') {|f| f << text } end end
With that code I can easily update my application's mail templates and manage them at MailChimp. My templates don't change often so I keep the update manual, but you could set up a cron job to pull in changes nightly, or create a Capistrano task to pull in a fresh copy with each application deployment.
So what about tracking? That was the last wish on my list and was easily gotten with a nifty SMTP service called SendGrid. Goodbye Gmail! SendGrid works extra hard to give my application a high email delivery rate and it provides a nice web interface for viewing all email sends, clicks, bounces, etc. SendGrid's tracking isn't as slick as MailChimp's, but it's a heck of a lot better than nothing. Overall I've been very pleased with SendGrid's service, and happy as a clam with my MailChimp'd ActionMailer templates.
- In response to Amazon's recent foray into transactional email MailChimp created a service on top of SES. ↩
Incoming search terms:
- sendgrid templates
- rails transactional email
- sendgrid email templates
- mailchimp rails
- sendgrid mailchimp rails
- rails mailchimp tutorial
- mailchimp transactional emails
- sendgrid mailchimp
- rails mail client
- tracking emails sent through rails application using mailchimp


text:
Stephen Chantry Still amazing…"On The Run!"
This entry was posted on Wednesday, October 3rd, 2012 at 12:29 pm and is filed under .
You can follow any responses to this entry through the RSS 2.0 feed.
You can leave a response, or trackback from your own site.
Name (required)
Mail (will not be published) (required)
All content © Copyright 2000 – 2014 WAVY Blogs. All Rights Reserved.


text:
Immunization Information for School
The information and forms are in .PDF format. The free Adobe Acrobat Viewer
is required to view them.
Ohio Department of Health
Phone: (614) 466-3543 ~ Contact Us
Address: 246 N. High St., Columbus, Ohio 43215
Personal health questions? Call your local health department
or your local health care provider. For emergencies, dial 911.


text:
"Jerry Avins" <[email protected]> wrote in message
news:[email protected]ps.com...
> Becca,
> I'm sorry 5to be tuning n late. I always brown my onions when I make
> tomato sauce for canning. The first thing in the pot is coarsely cut up
> onion in a bit of olive oil. In the electric kettle, I set the thermostat
> for about 230F and stir once in a while.It takes a good half hour to
> caramelize them, but the sweetness is worth it. (Some people just add
> sugar, but it's not the same.) The wet ingredients go in after. You can do
> a great French onion soup the same way. Low heat, long time works for me.
Not meant for me but taken on board!!! Thanks, Jerry!


text:
Shame about the stupidity.
Shame about the stupidity.
So yesterday I was returning from Scotland – more of that later – and traffic alerts and GPS guided me around a major motorway closure; the M6 near Stoke-on-Trent was closed due to a fatal motorcycle crash, traffic was stopped in the road for several hours whilst investigations were carried out – I believe the crash was towards 9am and the motorway was still closed well after lunch – and there were many, many motorists stuck in 27C+ temperatures on a hot road.
Another friend of mine was not so lucky – her choir was stuck in the midst of it – so they organised an impromptu concert on the motorway verge:
Glad I'm not the only person who's thought this:
11 Harsh Realities About The World Of Harry PotterWe're blowing the lid off this totalitarian regime! Once you peel back the layers of child-like wonder, it kind of sucks to be a wizard.
Been a bit quiet here, recently. Back now. Normal-ish service to be resumed over the next few weeks.
… part of a balanced supper.
Normality is being gradually restored.
Via BB
Kiwi (FTP archive search to Sun internally) and Ben-Nevis (AltaVista ripoff) – two of Sun's first-ever search engines, internally facing, lashed up in Perl and with (eventually) CGI interfaces; the graphics are from a public presentation on "What the Web Does" that I did, sometime around (I think?) 1995.
Repeat after me: state regulation is always a sane and desirable thing…
WHETHER the obscure statute that governs America's raisin trade is constitutional, Elena Kagan is not sure. She and her fellow Supreme Court justices are pondering that question at the moment, and will rule shortly. But she sounds reasonably confident that the Agricultural Marketing Agreement Act of 1937 is "the world's most outdated law".
Since the 1940s raisin farmers have been obliged to make over a portion of their crop to a government agency called the Raisin Administrative Committee. The committee, run by 47 raisin farmers and packers, along with a sole member of the raisin-eating public, decides each year how many raisins the domestic market can bear, and thus how many it should siphon off to preserve an "orderly" market. It does not pay for the raisins it appropriates, and gives many of them away, while selling others for export. Once it has covered its own costs, it returns whatever profits remain to farmers. In some years there are none. Worse, farmers sometimes forfeit a substantial share of their crop: 47% in 2003 and 30% in 2004, for example.
Participation in this Brezhnevite scheme is mandatory. […]
Read the rest at America's raisin regime: De minimis curat lex | The Economist.


text:
All ornaments are mint-in-box unless otherwise noted. Some boxes have the original price tags taken off the sides of the boxes, so if this matters to you, please inquire if the ornament you want has it or not. We have duplicates of some ornaments so if you are interested in more than one, please ask.
Click on thumbnail pictures for a better view, then hit the back button to return
Miscellaneous Hallmark Ornaments
|1978 Merry Miniatures Pilgrim Boy - loose, no box...$10
|1995 Star Trek Captain James T. Kirk (William Shatner)...$20
|1995 Beverly and Teddy...$25
|1997 Happy Christmas to All! - for Collector Club members only...$10
|1997 Away to the Window - for Collector Club members only...$10
|1998 Our First Christmas Together...$8
|1998 Lapel Pin - based on Hallmark's 1998 "Night Watch" ornament, still on original card, card has small creases, pin is mint...$5
|1999 Mary's Bears...$8
1999 Arctic Artist – club membership ornament…$8
|2000 Ringing Reindeer - club membership ornament….$8
|2001 Betty Boop Santa's Little Sweetheart - Carlton Cards, Betty says "Boop Oop A Doop" includes cell batteries, although never displayed a tiny amount of paint has rubbed off on Betty's legs...$25
|2001 A Magical Dress for Briar Rose - from Walt Disney's Sleeping Beauty, dress changes from pink to blue... $15
|2001 Samantha "Sam" Stephens Bewitched...$15
|2001 Beaglescout - #4 in the Spotlight on Snoopy series...$10
|2001 Marilyn Monroe - Charlton Cards, Showbiz Fantasy...$18
|2001 Rugrats - Charlton Cards, Angelica & Chuckie...$17
|2001 Pillsbury Doughboy - Charlton Cards, sound ornament, Doughboy giggles, tab to be removed before making work still intact so has not been tested, although never displayed there is what looks like a glue stain on the back of his hat...$20
|2001 What a Grinchy Trick. - Dr. Seuss' The Grinch ..$16
|2001 Rugrats 10th Anniversary ornament, "Hold onto your Hats" - Charlton Cards, Dil and Tommy on saucer sled, no box…$10
|2001 All-Sport Santa...$10
|2002 Frosty Friends - 23rd in series...$14
|2002 The Biggest Fan...$10
|2002 Strawberry and Pupcake Ornament, Carlton Cards, scented ornament...$20
|2003 Kris Kringle...$10
|2003 A Gift for Raggedy Ann - clip on ornament...$10
|2003 Hop on Pop Dr. Seuss – 5th in series, windup knob doesn't work…$5
|2004 Catch of the Day…$10
|2004 The Peanuts Games – set of 4 ornaments…$10
|2004 Lord of the Rings Frodo Baggins…$14
Hallmark Car & Vehicle Ornaments
|1995 Merry RV...$18
|1995 Batmobile...$20
|1997 Chevrolet Corvette...$18
|1997 Miniature Corvette...$8
|1997 The Flight at Kitty Hawk,1st in the "Sky's the Limit Series…$15
|1998 1917 Curtiss JN-4D "Jenny", 2nd in the "Sky's the Limit Series…$15
|2004 1966 Oldsmobile Toronado Coupe – 14th in series…$14
Hallmark Lionel Ornaments
|1996 700E Hudson Steam Locomotive - 1st in Lionel Train series, side box flap is torn about 1 1/4"...$20
|1997 1950 Santa Fe F3 Diesel Locomotive - 2nd in Lionel Train series...$20
|1998 Pennsylvania GG-1 Locomotive - 3rd in Lionel Train series...$20
|1999 746 Norfolk and Western Steam Locomotive - 4th in Lionel Train series...$20
|1999 The Tender Lionel 745 Norfolk and Western -complements the 746 Norfolk and Western Steam Locomotive...$15
|2003 4449 Daylight Steam Locomotive - 8th in Lionel Train series...$20
|2003 Lionel Daylight Oil Tender - complements the 4449 Daylight Steam Locomotive...$15
|2003 Lionel Daylight Observation Car - complements the 4449 Daylight Steam Locomotive...$15
Hallmark Barbie Ornaments
|1994 Barbie Swimsuit - 1st in series...$20
|1994 Barbie Holiday - 2nd in series...$20
|1995 Barbie Holiday - 3rd in series...$20
|1997 Barbie Holiday - 5th in series...$18
|1995 Barbie Springtime- 1st in series...$15
|1996 Barbie Springtime - 2nd in series...$15
|1997 Barbie Springtime...$15
|1996 Native American Barbie...$15
|1997 Chinese Barbie - 2nd in Dolls of the World series...$15
|1997 Rapunzel...$15
|1997 Barbie Wedding Day...$18
|1997 Holiday Traditions Barbie...$15
|1997 Victorian Elegance Barbie....$15
|1999 40th Anniversary Barbie....$15
All photos on this site are copyrighted and may not be used without the written permission of Toys of Another Time, L.L.C.


text:
Policies and Guidelines
Facilities operated by University Recreation are in compliance with the Americans with Disabilities Act. Our staff is eager to provide assistance when needed. We want all our patrons to fully utilize our facilities and reach their health and fitness goals.
- Appropriate athletic attire must be worn in the Cardio, Weight and Fitness area at all times.
- Appropriate attire is defined by t-shirts or tanks that cover the torso to include the midriff and rib cage; pants or shorts made of light, breathable fabric; and athletic shoes that cover the entire foot.
- Clothing with exposed rivets, buttons, zippers, or other metal parts is not permitted.
- Food, gum, tobacco products or open beverage containers are prohibited.
- Only twist top or water bottle containers are permitted.
- Please re-rack weights in proper place when finished.
- Dropping or slamming weights is prohibited.
- Free weights, such as dumbbells and weight plates, must stay in the weight room at all times.
- Please wipe down equipment after use. Premoistened towels are provided.
- Use of a spotter and collars on bars is strongly recommended.
- Please ask the weight room staff if you would like assistance on how to use a piece of equipment.
- Please do not place personal items on or around the desk or exercise areas. Storage space is available under the TVs in the cardio area.
- Children 13 years and under are not permitted in the Cardio, Weight, & Fitness Area and/or Circuit Room.
- Report any damaged equipment, accidents, or misconduct to the weight room staff.
- Only UREC Personal Trainers are allowed to train clients.
- Dependents are only allowed in activity areas if they are participating in that activity and as such, must have a valid UREC membership. All age restrictions will apply for use of an activity area.
- Dependents that are not participating in an activity must remain in the 1st floor lounge area and must be directly supervised (within reaching distance) by a parent or guardian at all times. As such, the dependent would not be required to hold a UREC membership or pay the guest fee.
- Only parents or legal guardians, who are current members, may purchase a membership for their dependents (18 years of age and older).
- If currently enrolled in school, dependents up to the age of 24 are also eligible for membership.
- Members 17 years of age and under may not sponsor a guest.
- Children six years of age and older are not allowed in opposite sex locker rooms.
|Over 16
|may utilize the facility without a parent or guardian directly present.
|14 – 16
|must be supervised (remain in the facility) by a parent or guardian at all times. If using the Cardio, Weight, & Fitness Area and/or Circuit Room the parent must supervise indirectly (in the same area) at all times.
|13 & under
|not permitted in the Cardio, Weight & Fitness Area
|6 – 13
|must be indirectly supervised at all times.
|Under 6
|must be directly (within reaching distance) supervised by a parent or guardian at all times. If using the pool area the parent or guardian must remain in direct contact with the child at all times. If in opposite sex locker-room, children 5 years and younger must be changed in one of the closed-door locker bays and use a shower with a curtain. The parent or guardian must make every attempt to keep exposure of the child to a minimum.
All UREC patrons are expected to be responsible, courteous, and safety conscious at all times. Disorderly conduct, abuse or misuse of the facility or its equipment, or disregard for policies will not be tolerated. Violators will be required to leave the facility and may be subject to disciplinary action. Violators of UREC policies may also be suspended from using the facilities. Members who are suspended will not have any portion of their fees refunded.
- University Recreation reserves the right to refuse admittance.
- Entry to and exit from the facility must be through controlled access points only. Other exits should be used for emergencies only.
- All members must present a valid LSU ID card or an UREC membership card upon entry.
- Beginning on June 9, 2014: To provide better service to students and members, UREC will extend ID forgiveness to those members who do not have a physical photo ID, but have their photo in Fusion (UREC's membership management program). ID forgiveness will be limited to 3 times per semester.
- Gum, tobacco, alcoholic beverages, and pets are not allowed in UREC facilities.
- Proper attire is required at all times. Shirt and shoes are required at all times except in the locker rooms and pool area.
- University Recreation is not responsible for lost or stolen items.
- Membership privileges may be revoked or suspended for policy violation at any time.
- A $15 replacement fee is assessed for lost membership cards.
- University Recreation may not be used for solicitation, private classes, personal training, or any other activity associated with personal gain.
- Rules for specific areas are posted on site. These rules may include more area specific rules that are not listed in the General Rules.
- No type of sport activities will be allowed in hallways of the SRC.
- All policies outlined in the Student Code of Conduct will be enforced. The Student Code of Conduct" can be found on the LSU Web site under the Dean of Students.
- Photography is permitted in LSU UREC facilities for personal non-commercial and lawful use provided permission has been granted by the individual(s) being photographed. The taking, capturing or recording of any and all photography, audio, or video of any kind in the pool, men's and women's locker rooms, and restrooms is strictly prohibited.
- Those who seek to photograph, film, or interview individuals at LSU UREC facilities for commercial use, media use, or class projects, must receive prior written approval from University Recreation administration prior to accessing LSU UREC facilities.
- By entering a LSU UREC facility, you consent to being the subject of any photography, audio, or video recordings, which may take place while you are participating in programming and/or open recreation activities. Such photography and recordings may be used for LSU publications, webcasts, telecasts, advertising, and for any other additional promotional or marketing purpose as Louisiana State University may see fit. By entering a LSU UREC facility, you hereby waive all rights or claims you may have to any financial compensation or payment of royalties in connection with any publications, webcasts, broadcasts, or exhibition of these materials. When/if your likeness or image is used in a publication, there will be no identifying information provided. If you do not wish to be photographed, please kindly inform our photographer or videographer.
- Without a valid UREC membership, any individual who wishes to utilize UREC facilities or programs must purchase a day pass and be sponsored by a UREC member, regardless of age.
- Individuals must present a valid government-issued photo ID in order to purchase a day pass.
- Anyone eligible for an UREC membership may purchase a day pass with valid photo identification. Should Identification be falsified in any way, individuals can be banned from LSU UREC facilities and programs for life.
- Individuals staying at either the Lod Cook Hotel & Conference Center or Faculty Club are allowed entry with the purchase of the $10 day pass by presenting a room key or room receipt.
- Each guest must present valid photo identification upon entry.
- Members are allowed up to three (3) guests per day at the rate of $10 per guest.
- Day passes may be purchased with cash or TigerCash.
- The sponsor may not leave LSU UREC facilities/property without his/her guest.
- Members are responsible for the conduct of their guest(s) and are sub ject to all rules and regulations affiliated with a day pass, including membership termination should their guest's conduct warrant.
- Under-age members (17 years of age and under) may not sponsor a guest.
- Guests or day pass holders may not check-out equipment, make court reservations, or participate in Intramural programs.
- Day passes will not be sold within one hour of closing time.
- Day passes are non-transferable and non-refundable.
- Spectators associated with facility rentals are only permitted in the UREC SRC for authorized and specials events as per the facility rental contract.
(Group Rental participants/spectators must check in at the UREC Operations Desk to obtain the appropriate pass to enter the facility)
LSU Family Association
Members of the LSU Family Association receive two (2) free guest passes and are eligible to purchase a UREC membership at the affiliate rate. Those who visit campus less frequently and would like access to UREC facilities can purchase a daily pass for just $10 per person by showing their current Family Association membership card. The Family Association member must be present to be eligible for this offer and will be required to present a photo id (driver's license, government-issued ID).
- Any individual wishing to rent a locker must possess a current UREC membership. UREC members must present a valid TigerCard or UREC membership card at time of purchase.
- With purchase of a semester or annual locker plan, each member will receive an assigned locker, combination, and corresponding expiration date.
- All lockers must be cleared out at the end of each rental period.
- An email notification will be sent to each member (provided an email address is on file with LSU UREC) one month prior to the expiration date. In addition, expiration notices will be posted on the lockers two weeks in advance of the end of each rental period.
- All lockers must be renewed or cleared by the semester or annual expiration date. Should you elect not to renew your locker during the renewal period, please remove all locker contents and place the lock back on your locker once finished. Failure to clear the locker by the stated date will result in UREC staff clearing the locker and assessment of a $10 service fee.
- All property left in the locker will become the property of LSU University Recreation and will subsequently be donated to charity if not claimed from the UREC Equipment Desk within 30 days.
- All lockers are the exclusive property of LSU UREC and as such may, without prior notice, be opened for operational and/or maintenance purposes.
- It is recommended that all patrons and guests leave no valuables, such as money, wallets, watches, jewelry, etc., unattended in their lockers.
- Patrons should double check the lock to make sure it is locked when showering or leaving the facility.
- It is recommended for safety reasons that participants with health problems check with a physician before using the sauna. Always sit covered with a towel or wear clothing while in the sauna.
- When showering, do not leave any soap or shampoo in the shower area.
- Personal locks are not to be used at any time, and will be cut off.
- Lost and found articles may be taken to the Equipment Desk.
- Do not leave any doors or entrances into the locker rooms propped open.
- All thefts, vandalism or unusual situations should be reported to the UREC Supervisor – Facilities.
- Children 6 years of age and older are not allowed into an opposite sex locker-room.
- If in opposite sex locker-room, children 5 years and younger must be changed in one of the closed-door locker bays and use a shower with a curtain. The parent or guardian must make every attempt to keep exposure of the child to a minimum.
Clothes, equipment, and other miscellaneous items are stored in the Equipment Room. ID cards can be turned in at the UREC Operations Desk and will be secured by the manager on duty.
Parents of current LSU UREC student members are eligible to receive two (2) free guest passes to use UREC facilities and programs*. The policy allows current LSU UREC student members the ability to extend two (2) free guest passes to their parents (step parents) or guardians per semester.
- Each student member is allotted 2 free passes per parent per semester (includes fall, spring, & summer)
- The passes will renew at the beginning of each semester the student is considered a member of LSU UREC
- The student and parent must check in at the UREC operations desk to process.
- Student must be present to receive the passes.
- All rules and guidelines associated with the LSU UREC guest pass policy are applicable.
* Program use excludes League and Tournament participation
By participating in or using any activities, programs, equipment or facilities available through LSU University Recreation participant/user hereby agrees to the following terms and conditions:
I understand and agree that there is a risk of serious injury to me while utilizing LSU UREC facilities, equipment, and programs and recognize every activity has a certain degree of risk, some more than others. By participating, I knowingly and voluntarily assume any and all risk of injuries, regardless of severity, which from time to time may occur as a result of my participation in athletic and other activities through LSU University Recreation.
I hereby certify I have adequate health insurance to cover any injury or damages that I may suffer while participating, or alternatively, agree to bear all costs associated with any such injury or damages myself.
I further certify that I am in good health and have no mental or physical condition or symptoms that could interfere with my safety or the safety of others while participating in any activity using any equipment or facilities of LSU University Recreation. I understand and agree that I alone am responsible to determine whether I am physically and mentally fit to participate, perform, or utilize the activities, programs, equipment or facilities available at Louisiana State University, and that I am not relying on any advice from LSU University Recreation in this regard. To the extent I have any questions or need any information about my physical or mental condition or limitations, I agree to seek professional advice from a qualified physician.
Further, I hereby RELEASE AND HOLD HARMLESS, the State of Louisiana, the Board of Supervisors of Louisiana State University and Agricultural and Mechanical College, and its respective members, officers, employees, student workers, student interns, volunteers, agents, representatives, institutions, and/or departments from any and all liability, claims, damages, costs, expenses, personal injuries, illnesses, death or loss of personal property resulting, in whole or in part, from my participation in, or use of, any facility, equipment, and/or programs of Louisiana State University.
By entering a LSU UREC facility, I consent to being the subject of any photography, audio, or video recordings, which may take place while I am participating in programming and/or open recreation activities. Such photography and recordings may be used for LSU publications, webcasts, telecasts, advertising, and for any other additional promotional or marketing purpose as Louisiana State University may see fit. By entering a LSU UREC facility, I hereby waive all rights or claims I may have to any financial compensation or payment of royalties in connection with any publications, webcasts, broadcasts, or exhibition of these materials. When/if my likeness or image is used in a publication, there will be no identifying information provided. If you do not wish to be photographed, please kindly inform our photographer or videographer.
UREC membership and program registration are paid in full at the time of purchase.
- A refund for membership cancellation may be granted for the following circumstances:
- Injury/Illness with doctor's note provided
- Departure from University employment
- All refunds require a $10 administrative charge and the approval of the LSU University Recreation Associate Director of Business Administration. Refunds may take up to 10 business days to process.
- Written notices of all requests for cancellation are required. Notice to request cancellation should be sent to firstname.lastname@example.com.
- A full refund will be granted for program cancellations due to insufficient enrollment and will not result in a $10 administrative charge.
Visitors are considered anyone coming to UREC to either meet with a UREC staff member or to serve as a spectator during a UREC programmed activity. Visitors must check-in at the Operations Desk and the applicable staff member will be contacted to escort visitors to the appropriate meeting or UREC programmed activity in the facility.


text:
Outsourcing Guide
Cont. Education
Training Courses
Web Seminars
Buyer's Guide
Home Page
Pharm Patents
Pharm News
Federal Register
Pharm Stocks
FDA Links
FDA Warning Letters
FDA Doc/cGMP
Pharm/Biotech Events
Newsletter Subscription
Web Links
Site Map
Title: Live genetically
attenuated malaria vaccine
United States Patent: 7,122,179
Issued: October 17, 2006
Inventors: Kappe; Stefan H.
I. (Seattle, WA), Matuschewski; Kai-Uwe C. (Heidelberg, DE), Mueller;
Ann-Kristin (Dossenheim, DE)
Assignee: Seattle
Biomedical Research Institute (Seattle, WA)
Ruprecht-Karls-Universitat Heidelberg (Heidelberg, DE)
Appl. No.: 11/078,200
Filed: March 11, 2005
Outsourcing Guide
Method for inoculating a vertebrate host
against malaria, by administering to the host a live Plasmodium organism
that is genetically engineered to disrupt a gene whose expression is
up-regulated in liver stage parasites and whose function is not required
for entry into hose hepatocytes.
Here we show by reverse genetics that
selected individual genes, exemplified by UIS3 (up-regulated in infective
sporozoites gene 3) and UIS4, are essential for early liver stage
development: uis3(-) and uis4(-) sporozoites infect hepatocytes but are no
longer able to establish blood stage infections in vivo and thus do not
lead to disease. The invention thereby provides the first live Plasmodium
organisms that are genetically engineered to disrupt liver-stage-specific
gene functions.
Surprisingly, immunization with either uis3(-) or uis4(-) sporozoites
confers complete protection against infectious sporozoite challenge in a
rodent malaria model. This protection is sustained and stage-specific.
These findings provide the first genetically attenuated whole organism
malaria vaccines.
Thus, the invention provides a method for inoculating a vertebrate host
against malaria, by administering to the host a live Plasmodium organism
that is genetically engineered to disrupt a liver-stage-specific gene
function. The invention further provides a vaccine composition comprising
a live Plasmodium organism that is genetically engineered to disrupt a
liver-stage-specific gene function. In addition, the invention provides
the use of a vaccine composition comprising a live Plasmodium organism
that is genetically engineered to disrupt a liver-stage-specific gene
function. The invention also provides for production of a vaccine
composition, by suspending and packaging the subject engineered Plasmodium
organisms in a suitable pharmaceutically acceptable carrier solution.
The invention provides a method for
inoculating a vertebrate host against a Plasmodium parasite, by
administering to the host a live Plasmodium organism that is genetically
engineered to disrupt a liver-stage-specific gene function.
By "Plasmodium parasite" or "Plasmodium organism" is meant any member of
the protozoan genus Plasmodium, including the four species that cause
human malaria: P. vivax, P. malariae, P. falciparum, and P. ovale. The
corresponding "vertebrate host" is a human or other secondary host that is
susceptible to infection by the wild-type Plasmodium parasite.
For use as a live anti-malarial vaccine, the Plasmodium parasite is
genetically engineered to disrupt a liver-stage-specific gene function.
The term "disrupt liver-stage-specific gene function" or "disrupt
LS-specific gene function" means interfering with an LS-specific gene
function such as to completely or partially inhibit, inactivate,
attenuate, or block the LS-specific gene function, for example, by gene
disruption or influencing transcription, translation, protein folding,
and/or protein activity. The term "liver-stage-specific gene function" or
"LS-specific gene function" refers to a function that is required in liver
stage parasites to ultimately produce infectious merozoites and establish
the erythrocytic stage of the life cycle, but that is not required for
entry into host hepatocytes or, preferably, maintenance of the parasite in
asexual blood cell stages and production of infective sporozoites in
Malaria infection is initiated by Plasmodium sporozoites in the salivary
glands of mosquitoes. These sporozoites invade hepatocytes of the
vertebrate host and differentiate into liver stage (LS) forms. After a few
days the LS parasites produce several thousand merozoites that are
released from the hepatocytes and invade erythrocytes to start the blood
stage cycle that causes malaria disease. According to the invention, the
Plasmodium parasite is genetically engineered to disrupt at least one
LS-specific gene function such that the genetically engineered parasites
remain capable of invading hepatocytes but cannot produce merozoites that
can establish blood stage infections. Of course, pursuant to this
disclosure, more than one LS-specific gene function can be disrupted (such
as by creating, for example, double knock-outs) as such redundancy may
ensure an additional degree of protection against parasitemia.
Pursuant to this disclosure, an LS-specific gene function may be
identified using routine methodology that is standard in the art. For
example, an LS-specific gene function may be identified by assessing the
function of genes whose expression is up-regulated in liver-stage
parasites ("LS-up-regulated genes"). For example, genes whose expression
is up-regulated in liver-stage parasites may be expressed at higher levels
in liver-stage parasites than, e.g., in the sporozoite population that
emerges from mosquito mid-gut oocysts. Up-regulation of expression of such
genes may also be observed in mature, infective salivary gland sporozoites
(like in the UIS4 and UIS3 genes discussed in the Examples below).
Well-known methods for differential transcriptional profiling, including,
but not limited to, subtractive hybridization screens, differential
display, and genome-wide microarray analyses, may be used for identifying
genes whose expression is up-regulated in liver-stage parasites. Such
methods have been previously used to analyze infectivity-associated
changes in the transcriptional repertoire of sporozoite-stage parasites
(11) and to identify Plasmodium genes that encode pre-erythrocytic
stage-specific proteins (12). For example, suppression subtractive
hybridization permits selective enrichment of differentially regulated
cDNAs of high and low abundance through a combination of hybridization and
polymerase chain reaction (PCR) amplification protocols that allow the
simultaneous normalization and subtraction of the cDNA populations.
Suppression subtractive hybridization has been used to analyze
transcriptional differences between non-infective and infective
sporozoites and to identity genes controlling infectivity to the mammalian
host (11). This procedure has permitted the identification of
LS-up-regulated genes, including but not limited to the UIS3 and UIS4
genes disrupted in the Examples below. Suppression subtractive
hybridization of Plasmodium salivary gland sporozoites versus merozoites
has also been used to identify stage-specific pre-erythrocytic transcripts
(12). Differential expression of candidate LS-specific genes may be
confirmed using methods that are standard in the art, including dot blots,
reverse transcriptase PCR (RT-PCR), immunoblotting, immunofluorescence
microscopy, and/or microarray expression analyses.
In some embodiments of the invention, LS-specific gene functions are
identified by analyzing the function of LS-up-regulated genes, as further
described below. However, not all genes with an LS-specific gene function
are necessarily LS-up-regulated genes. Thus, genes whose expression is not
up-regulated in LS forms may nevertheless possess an LS-specific gene
Interference with a liver-specific function may also be achieved by
LS-specific overexpression of an inhibitory factor. This factor may be
inserted by reverse genetics methods into a pseudogene, i.e., one that is
not essential for parasite survival at any time point during the life
cycle (47). The inhibitory factor should not confer toxicity to the
parasite but rather act in arresting LS development. Such a factor may
include, but is not limited to, inhibitors of cell-cycle progression
and/or ubiquitin-mediated proteolysis, and/or factors that interfere with
post-transcriptional control of gene-expression.
LS-specific gene functions may be identified by analyzing the phenotype of
parasites in which one or more gene functions have been disrupted. Several
methods for disrupting gene functions in Plasmodium are well-known in the
art and may be used in the practice of the invention. Such methods
include, but are not limited to, gene replacement by homologous
recombination, antisense technologies, and RNA interference. For example,
methods of gene targeting for inactivation or modification of a Plasmodium
gene by homologous recombination have been established (13). Such methods
were herein successfully used to disrupt LS-specific gene functions, as
described in Examples 1 and 2. Antisense technology has also been
successfully used for disrupting Plasmodium gene functions. For example,
exogenous delivery of phosphorothioate antisense oligonucleotides against
different regions of the P. falciparum topoisomerase II gene result in
sequence-specific inhibition of parasite growth (14). Similarly,
transfection of an antisense construct to the Plasmodium falciparum clag9
gene, which had been shown to be essential for cytoadherence by targeted
gene disruption, resulted in a 15-fold reduction in cytoadherence compared
to untransfected control parasites (15).
Another exemplary technology that may be used in the practice of the
invention to disrupt LS-specific gene functions is RNA interference (RNAi)
using short interfering RNA molecules (siRNA) to produce phenotypic
mutations in genes. RNAi has been used as a method to investigate and/or
validate gene function in various organisms, including plants, Drosophila,
mosquitoes, mice, and Plasmodium (see, e.g., 37 44). In Plasmodium, RNAi
has been used, for example, to demonstrate the essential role of a PPI
serine/threonine protein phosphatase (PfPP1) from P. falciparum (41). RNAi
has also been used to inhibit P. falciparum growth by decreasing the level
of expression of the gene encoding dihydroorotate dehydrogenase (42) and
by blocking the expression of cysteine protease genes (43). In the mouse
malaria model, RNAi has been used to inhibit gene expression in
circulating P. berghei parasites in vivo (44). These studies have
demonstrated the use of RNAi as an effective tool for disrupting gene
function in Plasmodium organisms.
The gene disruption approaches described above (for example, gene
targeting by homologous recombination, antisense, and RNAi) have been used
successfully to investigate the function of virtually all genes in an
organism's genome. For example, the availability of sequenced genomes has
enabled the generation of siRNA libraries for use in large-scale RNAi
studies to screen for genes that are involved in various processes, such
as developmental pathways or stages (see, e.g., 45 and 46). Such screens
may be used in the practice of the invention to identify LS-specific gene
functions in Plasmodium. Assays that may be used for identifying
LS-specific gene functions include, but are not limited to, phenotypic
analyses such as the phenotypic assays described in Examples 1 and 2. The
term "phenotypic analysis" includes all assays with vital recombinant
parasites that are generated in a wild type, fluorescent or any other
transgenic reporter background. Assays may be performed in vivo, with
cultured cells, in in vitro development assays or any other system that
provides a read-out for LS development.
The engineered Plasmodium organisms in which an LS-specific gene function
has been disrupted are typically grown in cell culture or animals,
expanded in the mosquito host, and harvested as sporozoites for use in
vaccines (see, e.g., 16).
The subject vaccine compositions are produced by suspending the attenuated
live Plasmodium organisms in a pharmaceutically acceptable carrier.
Suitable pharmaceutically acceptable carriers include sterile water or
sterile physiological salt solution, particularly phosphate buffered
saline (PBS), as well known in the art.
Vaccines according to the invention can be administered, e.g.,
intradermally, subcutaneously, intramuscularly, intraperitoneally, and
Dosage is empirically selected to achieve the desired immune response in
the host. By "immune response" is meant an acquired and enhanced degree of
protective immunity, preferably complete or sterile protection, against
subsequent exposure to wild-type Plasmodium sporozoites. In the working
examples described below, sterile protection was achieved following three
vaccinations with 10,000 live genetically attenuated sporozoites per
Background. Radiation-attenuated
sporozoites are a singular model that achieves sterile, protective
immunity against malaria infection.
Malaria causes more than 300 million clinical cases and more than 1
million death annually. The disease has a severe negative impact on the
social and economic progress of developing nations. Transmission of the
malaria parasite Plasmodium to the mammalian host occurs when infected
mosquitoes bloodfeed and inoculate the sporozoite stage (spz). After
entering the bloodstream, spzs are quickly transported to the liver where
they extravasate and invade hepatocytes (2). Within hepatocytes, spzs
transform into liver stages (LS) (also called exo-erythrocytic forms, EEFs).
LS parasites grow, undergo multiple rounds of nuclear division and finally
produce thousands of merozoites (17, 18). Merozoites released from the
liver rapidly invade red blood cells and initiate the erythrocytic cycle,
which causes malaria disease. A protective malaria vaccine would have
tremendous impact on global health but despite over a century of efforts,
no vaccine has been developed that confers prolonged protection. Yet, we
have known for more than 35 years that sterile protracted protection
against malaria infection is possible.
Immunization of mice with radiation-attenuated rodent model malaria spzs
(gamma-spzs) induces sterile immunity against subsequent infectious spz
challenge, thus completely preventing the initiation of blood stage
infection from the liver (5). Importantly, based on these findings it was
later shown that immunization of humans with gamma-P. falciparum spzs
completely protected greater than 93% of human recipients (13 of 14)
against infectious spz challenge and that protection can last for at least
10 months (6). Gamma-spzs retain the capacity to infect the liver of the
mammalian host and invade hepatocytes (19 20). However, LS derived from
gamma-spzs suffer arrested development and thus do not produce red blood
cell-infectious merozoites. Although, the inoculated stage is the spz, the
main immune target is the infected hepatocyte harboring the LS (21).
Protective immunity is spz-dose and radiation-dose dependent: greater than
1000 immunizing bites from P. falciparum-infected mosquitoes exposed to
15,000 20,000 rads of gamma radiation is required to protect the majority
of subjects exposed to infectious spz challenge (6). Mosquitoes inoculate
between 10 100 spzs during a bite (22 23). Therefore, the total spz dose
for complete protection comes to 10,000 100,000. Importantly, immunization
with over-irradiated spzs or heat-inactivated spzs fails to induce
protection, indicating that the spz must remain viable for some time after
inoculation and must progress to a liver stage that induces protection (6,
24). On the basis of observations in the rodent malaria model, protracted
protective immunity may depend on sufficient expression of LS antigen
(Ag), because treatment with primaquine, a drug that kills LS, aborts the
development of protection (21). Importantly, protection induced by P.
falciparum gamma-spzs is strain-transcending: inoculation with gamma-spzs
of one parasite strain confers protection against heterologous strains
Although we have learned much about spz gene expression in the last few
years (25 27), the LS as the putative immunological target(s) of gamma-spzs
induced protection have so far completely eluded gene expression analysis
because of their inherent experimental inaccessibility. We currently know
only one liver stage-specific Ag, liver stage antigen-1 (LSA-1) (28).
Thus, the fine Ag specificity of lymphocytes participating in protective
immunity remains unknown in humans, because the Ags expressed by LS
parasites remain unknown.
Feasibility to create genetically attenuated Plasmodium Liver Stages. To
generate genetically attenuated Plasmodium LS that are defective only in
LS development a stage-specific gene that plays an essential and exclusive
role at this stage needs to be disrupted. The gene should not be essential
during the blood stage cycle given that Plasmodium is haploid and
transfection is done with asexual blood stages and the mutant parasites
are typically maintained as blood stages (13). We previously employed
transcription-profiling based on the prediction that infectious Plasmodium
spzs residing in the mosquito salivary glands are uniquely equipped with
transcripts required for hepatocyte invasion and subsequent development of
the LS (11). Next, we screened for transcripts that are specific for pre-erythrocytic
and absent from blood cell stages (12). The combined screens identified
two abundant salivary-gland-spz-enriched transcripts that are absent from
blood stages, termed UIS3 and UIS4 (for upregulated in infectious spzs).
Cell biological studies have shown that both encoded proteins locate to
the parasitophorous vacuole, the parasite-derived organelle where
replication and schizogony takes place (data not shown).
Gene knockouts using insertion and replacement strategies have now
revealed that both genes are necessary for LS development (see Examples 1
and 2 below). Both proteins are normally expressed in spzs (data not
shown), but uis3(-) and uis4(-) parasites develop normal spzs and these
invade hepatocyte normally. However, uis3(-) and uis4(-) LS arrest in
intermediate-LS development and do not produce late LS (data not shown).
Therefore, both UIS3 and UIS4 have LS-specific gene functions. Remarkably,
animals infected by natural bite or intravenously with doses of up to
10,000 spzs do not become patent, confirming that both genes play vital
roles in successful completion of the Plasmodium life cycle (see Tables 1
and 2 below). Therefore, we succeeded in generating the first genetically
attenuated LS. Based on these discoveries we and others can now advance
and test various LS-up-regulated genes identified by microarray analysis
for their importance in LS development. We predict that more
LS-up-regulated genes will turn out to be essential for LS development
(i.e., to possess LS-specific gene functions), especially uniquely
expressed genes given the remarkable capacity of the parasite to develop
from a single spz to more than 10,000 daughter merozoites. Such
LS-up-regulated genes can be similarly disrupted to produce additional
live vaccine candidates, as described herein.
Claim 1 of 1 Claim
1. A method for inoculating a
vertebrate host against malaria, comprising administering to the host a live
Plasmodium organism that is genetically engineered to disrupt a gene whose
expression is up-regulated in liver stage parasites and whose function is
not required for entry into host hepatocytes.
If you want to learn more
about this patent, please go directly to the U.S.
Patent and Trademark Office Web site to access the full


text:
Some advice about George Clooney
I have some advice for the two people who will be selected to go to a party for the President at George Clooney's house:
Choose your guest wisely. Whoever you pick to join you is going to owe you big time.
Think about it—and chip in $3 or whatever you can today to be automatically entered.Official Rules & restrictions on eligibility. Obama for America, 130 E. Randolph Chicago, IL 60601


text:
Mana while not tanking?
All things related to the expansion
Moderators: Fridmarr, Worldie, Aergis
I am curious how people are getting 2 mins. In fights where you have to OT with the intent of being behind the MT in threat and ahead of the other DPS : 1 min and there goes your mana cause you have to use all your threat abilities. Pot might give you a little bit more.
- melange
- Posts: 37
- Joined: Wed Apr 11, 2007 8:07 pm
Using Seal of Blood I can keep going for quite a while before OOMing...
Turn In, an NPC interaction automator - http://wow.curse.com/downloads/wow-addo ... rn-in.aspx
- Moderator
- Posts: 10495
- Joined: Mon May 14, 2007 9:24 am
Who is online
Users browsing this forum: No registered users and 0 guests


text:
Search Result
Search Tags: Paula Wolfson
Mobile device use causing nearsightedness in children
Doctors think increased use of mobile devices by children is causing a growing number of cases of nearsightedness.
Tags: myopia, national eye institute, National Institutes of Health, thomas yau,
Plastic surgery on the rise for Americans
A lot of Americans are shelling out big money for a little -- or not so little -- nip and tuck.
Tags: plastic surgery, Joseph Michaels
Certain health conditions during pregnancy increase women's risk of heart problems later
More women die of heart disease than men. And while the risk factors are pretty much the same for both sexes, some conditions during pregnancy can increase a woman's chances of heart problems later on.
Tags: heart attack, Stroke, cardiovascular disease,
A battle with bulimia: Overcoming the disorder
Laura Porter was a high school senior when she developed an eating disorder.
Tags: Laura Porter, bulimia, eating disorders, binge eating
Wounded veteran to snowboarding champion
In 2007, Capt. Wayne Waldon lost his right leg on the battlefield in Iraq. Now, he competes in snowboarding championships.
Tags: Wayne Waldon, snowboarding, Walter Reed, paralympics,
Poll: One in 4 head to work even when sick
Colds and flu often spread like wildfire in the workplace, and one big reason is that too many people who are sick just won't stay home.
Former child of war getting a chance to make a dream come true
He was one of the lost boys of Sudan. But years later, Joseph Rufino has found his way, and his lifelong dream of becoming a doctor is finally coming true.
Tags: South Sudan, Grenada, medical school,
Study suggests alcohol may affect men trying to conceive
It's well known know how important it is for pregnant women to stop drinking, but now there are signs it may be a good idea for would-be dads to quit drinking, too.
Tags: pregnancy, alcohol, fetal alcohol syndrome, Men, FAS
Too many supplements could inhibit workout success
When it comes to vitamins, it's possible to get too much of a good thing.
Tags: exercise, supplements, vitamins,
Vitamin C may help prevent stroke
That morning glass of orange juice may just be a powerful weapon to prevent stroke.
Tags: vitamin c, Stroke, hemorrhagic stroke, American Academy of Neurology, Pontchaillou University Hospital,Listen
Hi: 52 °F | Lo: 30 °F


text:
So....I've cried mutiple times today and dad tried pretty much harassing me to figure out what was wrong. He figured out it was *him* that was upsetting me and kinda left it at that. He pissed me off tho cuz for a while he just wouldn't let it go. He was all like "you get mad at me for never lsitening to you and when I finally sit down and want to talk you won't".....well reality check. This is not something to talk about with my FATHER
Post a comment in response:
|© 2002-2008. Blurty Journal. All rights reserved.