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Bernie Ecclestone could one day be back in charge of Formula 1 after revealing that he's "looking" into buying the sport.
The 83-year-old built Formula 1 into what it is today before selling it to private equity firm CVC.
Despite the sale, Ecclestone remained the man in charge until he was forced to step down earlier this year in the build-up to his bribery trial in Germany.
However, he may one day be back in power after revealing that he has held talks with CVC's Donald Mackenzie.
"I have spoken to Donald Mackenzie and I am looking at it," Ecclestone told the Daily Express.
"It is possible, although one or two other companies are interested and I would not enter an auction. Age does not make it impossible. I feel no different to how I did 40 years ago.
"I have a contract with the company, not the shareholders. I have seen some rubbish about being pushed out but there is no-one lined up to take over."
And although praising CVC for all they have done for Formula 1, the F1 supremo says if he bought back the business he would not run it like a public company.
"Our company has been a very good investment for CVC and it would be a very good investment for me or anyone who owned it," he said.
"You can make money from it, so it would not be a problem for me to find financial backers. I run it now, but it wouldn't make any difference. It just means I would have to buy the shares. I think the company is a good solid business and a good investment.
"The past couple of years we have been running the business as if CVC were going to float the company.
"So we have run it like a public company, which has limitations. If I bought it I wouldn't think of that. I have always been used to doing things on the spot and, with a public company, it is always about board decisions.
"It can be restrictive, although I must say CVC have been super, they don't tell me how to run it. They have been super-supportive with all my troubles."
Ecclestone's future, though, is still up in the air pending the outcome of his bribery trial.
He added: "I have two jobs now. One the day job and the other, what I hope is a part-time job in Munich.
"I know that the prisons are full of people who say they are innocent and this is why I am attending in Germany every week because I know I am innocent and I want to clear my name.
"I don't know what the outcome will be but there is no point in worrying. I would rather that bad things don't happen, I would like to think that I can continue to do what I do as long as I am capable.
"Getting locked up would concern me much more than losing control of F1."


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subset: commoncrawl


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We are a community based bicycle company in the heart of Ealing. We offer maintenance courses, second hand bikes, cycle instruction and a host of other services. We're mobile and offer on-site training, maintenance and bicycle for both private, community, educational or corporate entities.
We have a wider view than just Ealing and to this end we have a website www.londonbikehub.com to cater for this showcasing past projects and the wider range of services.
You're probably reading this page to find out about our maintenance courses. We've been running bike maintenance lessons for some time now and run courses for complete beginners to the more intense 2-day Park Tool course. We aim to demystify the bicycle so you feel more confident to keep your bike running in good working order. The introduction course is 4 hour session covering spotting problems on the bike, rear wheel removal, punctures and general advice on cycling.
We also sell second bikes and accept donations for recycling. Donated bikes are usually worked on by volunteers from our projects including homeless, summer activities on estates etc. Bikes that are beyond repair are recycled for parts or into other items such artistic creations, garden items and a host of other products.


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Definitions for flamefleɪm
This page provides all possible meanings and translations of the word flame
Random House Webster's College Dictionary
flamefleɪm(n.; v.)flamed, flam•ing.
(n.)a portion of burning gas or vapor, as from ignited wood or coal.
Category: Chemistry
Often, flames. the state or condition of blazing combustion.
inflamed condition.
brilliant light; scintillating luster.
bright coloring; a streak or patch of color.
a bright reddish orange color.
Category: Fine Arts
intense ardor, zeal, or passion.
an object of one's passionate love; sweetheart.
Category: Informal
Computer Slang.an act or instance of angry criticism or disparagement, esp. on a computer network.
Category: Computers
(v.i.)to burn with a flame or flames; burst into flames; blaze.
to glow like flame; shine brilliantly; flash.
to burn or burst forth with strong emotion; break into open anger, indignation, etc.
Computer Slang.to behave in an offensive manner, esp. on a computer network; rant.
Category: Computers
(v.t.)to subject to the action of flame or fire.
Computer Slang.to insult or criticize angrily, esp. on a computer network.
Category: Computers
Origin of flame:
1300–50; ME flaume < AF, var. of flaumbe; OF flambe, earlier flamble < L flammula
Princeton's WordNet
fire, flame, flaming(verb)
the process of combustion of inflammable materials producing heat and light and (often) smoke
"fire was one of our ancestors' first discoveries"
flare, flame(verb)
shine with a sudden light
"The night sky flared with the massive bombardment"
be in flames or aflame
"The sky seemed to flame in the Hawaiian sunset"
criticize harshly, usually via an electronic medium
"the person who posted an inflammatory message got flamed"
Kernerman English Learner's Dictionary
the bright light from fire
flickering flames
on fire
The old school was in flames.
to start burning
The engine overheated and burst into flames.
The visible part of fire.
A romantic partner or lover in usually short-lived but passionate affair.
Intentionally insulting criticism or remark meant to incite anger.
A brilliant reddish orange-gold fiery colour.
The contrasting light and dark figure seen in wood used for stringed instrument making; the curl.
The cello has a two-piece back with a beautiful narrow flame.
To produce flames.
To post a destructively critical or abusive message, especially to provoke dissent or controversy
Of a brilliant reddish orange-gold colour, like that of a flame.
Origin: flaume, flaumbe, blend of flame and flambe, flamble, the first from flamma, the second from flammula, diminutive of flamma, both from pre-Latin *fladma; akin to gled.
Webster Dictionary
a stream of burning vapor or gas, emitting light and heat; darting or streaming fire; a blaze; a fire
burning zeal or passion; elevated and noble enthusiasm; glowing imagination; passionate excitement or anger
ardor of affection; the passion of love
a person beloved; a sweetheart
to burn with a flame or blaze; to burn as gas emitted from bodies in combustion; to blaze
to burst forth like flame; to break out in violence of passion; to be kindled with zeal or ardor
to kindle; to inflame; to excite
A flame is the visible, gaseous part of a fire. It is caused by a highly exothermic reaction taking place in a thin zone. Some flames, such as the flame of a burning candle, are hot enough to have ionized gaseous components and can be considered plasma. This subject is, however, hotly debated.
The New Hacker's Dictionary
[at MIT, orig. from the phrase flaming asshole] 1. vi. To post an email message intended to insult and provoke. 2. vi. To speak incessantly and/or rabidly on some relatively uninteresting subject or with a patently ridiculous attitude. 3. vt. Either of senses 1 or 2, directed with hostility at a particular person or people. 4. n. An instance of flaming. When a discussion degenerates into useless controversy, one might tell the participants "Now you're just flaming" or "Stop all that flamage!" to try to get them to cool down (so to speak).The term may have been independently invented at several different places. It has been reported from MIT, Carleton College and RPI (among many other places) from as far back as 1969, and from the University of Virginia in the early 1960s.It is possible that the hackish sense of 'flame' is much older than that. The poet Chaucer was also what passed for a wizard hacker in his time; he wrote a treatise on the astrolabe, the most advanced computing device of the day. In Chaucer's Troilus and Cressida, Cressida laments her inability to grasp the proof of a particular mathematical theorem; her uncle Pandarus then observes that it's called "the fleminge of wrecches." This phrase seems to have been intended in context as "that which puts the wretches to flight" but was probably just as ambiguous in Middle English as "the flaming of wretches" would be today. One suspects that Chaucer would feel right at home on Usenet.
British National Corpus
Nouns Frequency
Rank popularity for the word 'flame' in Nouns Frequency: #1666
Anagrams of flame
Translations for flame
Kernerman English Multilingual Dictionary
to burn with flames
His eyes flamed with anger.
- vlam; gevlamAfrikaans
- يَشْتَعِل غَضَباArabic
- пламтяBulgarian
- flamejarPortuguese (BR)
- planoutCzech
- leuchtenGerman
- flamme; blusseDanish
- φλέγομαι, βγάζω φλόγεςGreek
- arderSpanish
- leegitsemaEstonian
- شعله کشیدنFarsi
- liekehtiäFinnish
- jeter des flammes, flamberFrench
- לִבעוֹרHebrew
- आग की ज्वालाHindi
- plamsati, gorjetiCroatian
- égHungarian
- menyalaIndonesian
- logaIcelandic
- fiammeggiareItalian
- 燃えるJapanese
- 불꽃을 내며 타오르다Korean
- liepsnoti, degtiLithuanian
- liesmot; kvēlotLatvian
- bernyala-nyalaMalay
- vlammenDutch
- flamme, brenne, blusse oppNorwegian
- płonąćPolish
- شعله کشیدنPersian
- لمبى كولPashto
- flamejarPortuguese
- a se aprindeRomanian
- пылатьRussian
- blčaťSlovak
- goretiSlovenian
- plamtetiSerbian
- flamma, lysa, glödaSwedish
- ลุกไหม้Thai
- alev alev yanmakTurkish
- 發火焰Chinese (Trad.)
- палатиUkrainian
- شعلہ اگلناUrdu
- đốt cháy; cháy bùngVietnamese
- 发火焰Chinese (Simp.)
Get even more translations for flame »
Find a translation for the flame definition in other languages:
Select another language:
Discuss these flame definitions with the community:
Use the citation below to add this definition to your bibliography:
"flame." Definitions.net. STANDS4 LLC, 2014. Web. 19 Dec. 2014. <http://www.definitions.net/definition/flame>.


text:
A newborn's umbilical cord stump typically falls off within about two weeks after birth. In the meantime, treat your baby's umbilical cord stump gently.
Wonder how to care for your newborn's umbilical cord stump? Until the stump dries out and falls off, keep it clean and dry.
During pregnancy, the umbilical cord supplies nutrients and oxygen to your developing baby. After birth, the umbilical cord is no longer needed — so it's clamped and snipped. This leaves behind a short stump. The umbilical cord doesn't contain pain-sensitive nerve fibers, so your baby won't feel any discomfort during this rite of passage.
Your baby's umbilical cord stump will change from yellowish green to brown to black as it dries out and eventually falls off — usually within about two weeks after birth. In the meantime, treat the area gently:
During the healing process, it's normal to see a little crust or dried blood near the stump. Contact your baby's doctor if your baby develops a fever or if the umbilical area:
If your baby has an umbilical cord infection, prompt treatment can stop the infection from spreading.
Original article: http://www.mayoclinic.org/healthy-living/infant-and-toddler-health/in-depth/umbilical-cord/art-20048250
Any use of this site constitutes your agreement to the Terms and Conditions and Privacy Policy linked below.
Mayo Clinic is a not-for-profit organization and proceeds from Web advertising help support our mission. Mayo Clinic does not endorse any of the third party products and services advertised.
A single copy of these materials may be reprinted for noncommercial personal use only. "Mayo," "Mayo Clinic," "MayoClinic.com," "EmbodyHealth," "Enhance your life," and the triple-shield Mayo Clinic logo are trademarks of Mayo Foundation for Medical Education and Research.


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JAG: Season 10, Episode 3
Retrial (15 Oct. 2004)
Ratings: 7.5/10 from 41 users
Reviews: write review
In a retrial after 22 years Sturgis prosecutes, and Harm defends a man, a young seaman at the time, who was wrongfully convicted of murder. Meanwhile Mac prosecutes, and Bud defends a chief warrant officer with four wives and families.
Director:Jeannot Szwarc
Writer:Donald P. Bellisario (created by)


text:
Buck Pomerantz
All the information and links you'll need to plan, install, tune, and enjoy a subwoofer system in your car or truck.
Buck Pomerantz
Where should I mount my amp? What wiring do I need? Does any of it come with the amp? What size power and ground wires do I need for my amplifier? How much air space do I need around my amplifier? What's the biggest amp I can hook up to my car's electrical system? Plus more.
Video Guest
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Discgenics Successfully Completes Pilot Efficacy Study Of Injectable Discosphere™ Cell Therapy
SALT LAKE CITY, Feb. 28, 2013 /PRNewswire/ -- Discgenics announces the successful completion of an animal study demonstrating safety and efficacy of its novel therapy for reducing back pain caused by degenerative disc disease. This product, known as Injectable Discosphere Cell Therapy (IDCT), features Discgenics' patented, allogeneic, human disc-derived stem cell technology. The study showed that after one injection of IDCT in degenerated discs there was a restoration of disc height and tissue architecture, while noting no inflammatory response.
Discgenics CEO Flagg Flanagan said, " We are very encouraged by the results of the small animal pilot study as it shows the initial safety and efficacy of IDCT, which is produced from adult human disc-derived stem cells. We are optimistic that these early results will be indicative of our therapy's performance in further animal studies and will accelerate us toward human clinical studies and, ultimately, to an FDA cleared product. We believe this technology will revolutionize the way back pain is treated." Discgenics is currently conducting further studies of safety and efficacy in multiple animal species to support future scientific publications and for use in gaining regulatory clearance.
About Discgenics, Inc.
Discgenics is a privately funded spinal therapeutics company that is utilizing adult human disc stem cells within a tissue engineering approach to treat patients debilitated by degenerative disc disease. News and other information on the company are available at: http://www.discgenics.com .
SOURCE Discgenics, Inc.
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It's a tough question to answer: for the Buffalo Sabres, how do you answer the bell when you don't have a designated fighter to put in the ring?
But, you can't let your starting goalie get plowed and just take it, he knows that. Teams will roll over you all year if you set that precedent.
After Lucic creamed Miller everyone on the ice for the Sabres looked at each other and thought "someone's gonna fight him right? Please let someone who isn't me do this." They all looked to Gaustad, who for whatever reason couldn't bring himself to do it. (A guess: fighting someone like Milan Lucic – or anyone, for me - isn't something you just say "eff it" and do. Usually it's something you have to talk yourself into, work up to, and Gaustad likely will.)
But what about a team that doesn't even have a guy like Gaustad, what do they do when a heavyweight does something egregious? Nothing says "don't hit our goalie" like sending out some middleweight to get thumped.
A lot of people want the "thugs" taken out of the league, but when your starting goalie gets smeared by a tough guy like that, apologizing that your goalie got in the way isn't the right response. They're nice to have on occasion.
Without one, your options kinda suck. You can crosscheck the guy in the mouth if you're really determined to inflict pain, but that's only gonna be a suspension, and put you on the tough guy's radar for next time (if you care about stuff like that). You can go mow over the other teams goalie in the "eye for an eye" mentality, but 99% of the time that means you're gonna end up fighting anyway. At least you may avoid the heavyweight that way, but a goalie run-off isn't a situation we need in hockey.
So in the end…do you have to have a tough guy on your roster?
There's only one alternative I've seen: the "never let 'em see you sweat" option employed by the Detroit Red Wings for the past five years or so.
You can't get us pissed, you can't get under our skin, we're an emotionless machine hell-bent on dismantling your team and getting the W. It's the Nick Lidstrom/Pavel Datsyuk way.
If you don't have the talent to employ that tactic and win, then we're right back where we started: you actually do need a tough guy.
The more I think about how to answer a situation where an opponent intentionally hurt one of your teammates, the more I realize you either need to be a damn good team, or have a guy on hand who can stick up for your team (ideally one who can actually play). At least for the time being, that role in hockey is still necessary.


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January 16, 2011
Lacey, Washington – The lush Northwest Washington border
between Canada and the United States and the gulf between
"normal" folks and those regarded as peculiar are often as
imperceptible as they are illusory. They are the stuff of
"Border Songs," which has its world premiere in February at the
State Theater.
Based on Olympia author Jim Lynch's novel, a 2010 Washington
State Book Award winner, "Border Songs" was adapted for the
stage by Bryan Willis and is a co-production by Saint Martin's
University Theatre Department and Book-It Repertory Theatre.
David Quicksall directs the production.
At the heart of the story, threading between the borders, is
unlikely hero Brandon Vanderkool, a naïve and dyslexic,
six-foot-eight misfit pushed into joining the U.S. Border
Patrol. While he'd rather bird-watch or tend the cows on the
struggling family dairy farm, his skills of observation make him
surprisingly adept at spotting and busting drug smugglers. A
finely-drawn cast of small-town characters and illicit
activities drive a plot that is both amusing and poignant.
Curtains open on "Border Songs" at 7:30 p.m. Feb. 25-27 and
March 2-5 at the State Theater, 204 4th Ave E, Olympia Tickets
can be purchased at the door and are $10; $6 for students,
seniors and members of the military. The March 2 show is
Saint Martin's University is an independent four-year,
Catholic coeducational university located on a 380-acre wooded
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one west of the Rocky Mountains. Saint Martin's University
prepares students for successful lives through its 22 majors and
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campus, and 650 more to its extension campuses at Joint Base
Lewis-McChord, Everett College, Olympic College, Centralia
College and Tacoma Community College.
Fore more information:
Judy Turner
Show's producer


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We have just completed a fallen tree roof repair in Peachtree City. A tree had fallen on our client's rental home and damaged the roof, soffit, and fascia. She needed the damage fixed as soon as possible so that no more further damage was caused from moisture getting into the roof. We have done many fallen tree roof repairs. We can remove the tree limbs and branches before cutting into the roof.
Soffit, Fascia, Decking, and Trusses Repair
We bid to cut out all the damaged lumber, decking, trusses, fascia, and soffit. From there we would replace it and rebuild it with new lumber then paint it to match. We first had to cut out all the damaged wood which included 1/8 fascia board, ⅜ plywood soffit, 2/6 trusses, and the plywood decking. We were expecting more damage to the trusses when we cut it open, and when it was finally exposed we were surprised that more were not broken.
We rebuilt everything exactly as it was before the tree hit it, beginning with the trusses, then the plywood decking. We then had to rebuild the framing for the soffit and fascia then install the soffit and fascia. When we were done, the repaired roof looked exactly like it did before the tree fell on it.
Excellent Roof Repair Results
This job turned out excellently. Both the rental owners and the tenant were very satisfied with the finished product of this job. We hope that the images provided will help you understand the damage done and the required repairs. It took one full day of work to complete this roof repair.


text:
Wale - Panamera (Remix)
Posted May 24, 2012 at 03:57 PM
Panamera (Remix) Wale
Editor Rating: HOTTTTT
User Rating:
Wale remixes Magazeen's "Panamera"
Stream Panamera (Remix), the newest drop from Wale. The cut was released on Thursday, May 24th, 2012. Things are looking better for Wale with each new release, which Panamera (Remix) has proven yet again. It's worthy addition to the impressive body of work Wale has been developing over the years. We're looking forward to hearing what's next.
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Let us know what you think, folks.
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Posted on Aug 6th, 2012 by Gabe Delahaye Comment Tweet
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(5 of 7 customers found this review helpful)
Same bad and good as HFT #95974
By Dan5954
from Sterling Heights, MI
Comments about Haul-Master LED Trailer Light Kit:
It seems this shares the same pros, cons, and price of HFT LED lights #95974; so go and read those reviews. I had hoped there were red side-lamps in the tail lights this being a different part number, but alas, there are none; they are only reflectors. (I wonder, can you get a ticket for no side lights?) Not worth the work to add bulbs behind the reflectors and to waterproof them either, will spend a little more elsewhere and get a set with everything right. I let the store manager know the problem, hope he notifies corporate.
Bottom Line No, I would not recommend this to a friend
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(2 of 2 customers found this review helpful)
No posts on side-marker lights
By ShadowHawk
from Richardson, Tx
Best Uses
These lights were purchased to replace a few cracked lights on my trailer. Unfortunately, the original lights' side markers mount to the trailer via a post on the back of the light. These from HF do not have a mounting post on the amber side-marker lights. No way to install them. They'll have to go back to the store. Otherwise, the tail/turn/backup/brake/ lamps seem to be fine. The side markers, though... useless.After reading a few other reviews - I also noticed that there are no LED bulbs behind the side-facing lens of the brake/turn (rear-most) lights. These shoud be lit!
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Personal Information
Academic Positions
Academic and Professional Awards
Research Interests
Protein-protein interactions
are the foundation of many biological processes. As
we enter the post-Genomic era, the elucidation of
such interactions becomes central to biological research.
My research is focused on studying protein-protein
interactions by developing and applying novel protein
biotechnology tools. In particular, I am interested
in the mechanisms of antibody-antigen recognition
and have concentrated on the following research goals:
Figure 1
Figure 2
Figure 3
Figure 4
Display technologies are a central part of antibody engineering as antibody discovery-isolation-optimization tools (Benhar 2001, Benhar 2007). Display technology is based on generating a large repertoire of potential binders (an antibody library) which contains million to billions of individual clones. Selective pressure, usually in the form of affinity selection is than applied to pan out the clones with desired specificities (Figure 5).
Figure 5
With regard to antibody technology, we had recently introduced the concept of "antibody arrays for functional genomics". We developed a novel approach for high-throughput screening of recombinant antibodies, based on their immobilization on solid cellulose-based supports. We constructed a large human synthetic single-chain Fv antibody library where in vivo formed complementarity determining regions were shuffled combinatorially onto germline-derived human variable region frameworks. The arraying of library-derived scFvs was facilitated by our unique display/expression system, where scFvs are expressed as fusion proteins with a cellulose-binding domain (CBD). Escherichia coli cells expressing library-derived scFv-CBDs are grown on a porous master filter on top of a second cellulose-based filter that captures the antibodies secreted by the bacteria. The cellulose filter is probed with labeled antigen allowing the identification of specific binders and the recovery of the original bacterial clones from the master filter (Figure 6). These filters may be simultaneously probed with a number of antigens allowing the isolation of a number of binding specificities and the validation of specificity of binders (Figure 7). We screened our library against a number of cancer-related peptides, proteins, and peptide–protein complexes and yielded antibody fragments exhibiting dissociation constants in the low nanomolar range (Azriel-Rosenfeld et al., 2004). Our new antibody phage library, called: the "Ronit 1 library" became a valuable source of antibodies to many different targets, and to play a vital role in facilitating high-throughput target discovery and validation in the area of functional cancer genomics. That potential was recently demonstrated when antibodies we isolated from the library were used to characterize a new HLA-A*0201-restrected CTL epitope of PAP, a putative tumor associated antigen relevant to prostate cancer. This epitope, designated as PAP-3 (ILLWQPIPV), induced CTLs capable to lyse in-vitro HLA-A*0201 and PAP-positive tumor cells. We isolated from the "Ronit 1 library" recombinant single-chain Fv antibodies against HLA-A*0201/PAP-3 complexes and confirmed by confocal microscopy the presentation of PAP-3 by tumor cells in the context of HLA-A*0201 molecules (Machlenkin et al., 2007) (Figure 8).
Figure 6
Figure 7
Figure 8
Further, we recently demonstrated the potential of our antibodies to be incorporated into antibody chips. In that study we described a simple yet efficient strategy for the production of non-DNA microarrays, based on the tenacious affinity of a carbohydrate-binding module (CBM, formally CBD) for its three-dimensional substrate, i.e., cellulose. Various microarray formats were described, e.g., conventional and single chain antibody and peptide microarray for serodiagnosis of HIV patients (Ofir et al., 2005) (Figure 9).
Figure 9
Figure 10
Figure 11
therapy encompasses a wide variety of different strategies,
which can be divided into direct or indirect approaches.
Direct approaches target tumor-associated antigens
by monoclonal antibodies (mAbs) binding to the relevant
antigens or by small molecule drugs that interfere
with these proteins. Indirect approaches rely on tumor-associated
antigens expressed on the cell surface with antibody–drug
conjugates or antibody-based fusion proteins containing
different kinds of effector molecules. To deliver
a lethal cargo into tumor cells, the targeting antibodies
should efficiently internalize into the cells. Similarly,
to qualify as targets for such drugs newly-discovered
cell-surface molecules should facilitate the internalization
of antibodies that bind to them.
Figure 12
Figuer 13
drug-carrying phage nanomedicines
Figure 14
Our model
target was Staphylococcus aureus, and the model drug
was the antibiotic chloramphenicol. We demonstrated
the potential of using filamentous phages as universal
drug carriers for targetable cells involved in disease.
Our approach replaces the selectivity of the drug
itself with target selectivity borne by the targeting
moiety, which may allow the reintroduction of nonspecific
drugs that have thus far been excluded from antibacterial
use (because of toxicity or low selectivity). Reintroduction
of such drugs into the arsenal of useful tools may
help to combat emerging bacterial antibiotic resistance
(Yacoby 2006).
Figure 15
Figure 16
C virus
Figure 17
Figure 18
A prime requisite for detailed biochemical studies of the protease and its potential inhibitors is the availability of a rapid reliable in vitro assay of enzyme activity. We have developed a novel assay for measurement of HCV NS3 serine protease activity for screening of potential NS3 serine protease inhibitors. Recombinant NS3 serine protease was isolated and purified, and a fluorometric assay for NS3 proteolytic activity was developed. As an NS3 substrate we engineered a recombinant fusion protein where a green fluorescent protein is linked to a cellulose-binding domain via the NS5A/B site that is cleavable by NS3. Cleavage of this substrate by NS3 results in emission of fluorescent light that is easily detected and quantitated by fluorometry (Figure 19). Using our system we identified NS3 serine protease inhibitors from extracts obtained from natural Indian Siddha medicinal plants. Our unique fluorometric assay is very sensitive and has a high throughput capacity making it suitable for screening of potential NS3 serine protease inhibitors (Berdichevsky 2003).
Figure 19
Using antibody phage display we turned to isolate single-chain antibodies (scFvs) that, as intracellular antibodies will inhibit NS3 within cells. A few year ago we reported that in addition to its role in the viral polyprotein-processing, the viral NS3 serine protease has been implicated in interactions with various cell constituents resulting in phenotypic changes including malignant transformation (Zemel 2001). NS3 is currently regarded a prime target for anti-viral drugs thus specific inhibitors of its activities should be of importance. With the aim of inhibiting NS3-mediated cell transformation we isolated and characterized eight anti-NS3 scFvs from a human synthetic scFv library. We investigated the phenotypic changes that NS3-expressing cells undergo upon intracellular expression of these antibodies in different subcellular compartments (intracellular immunization), assayed by their proliferation rate and their ability to grow anchorage independently. The intracellular location of NS3 and the scFvs were analyzed by immunofluorescent staining using confocal microscopy (Figure 20). We found that nuclear-targeted anti-NS3 intrabodies shuttled NS3 from the cytosol to the nucleus with concomitant inhibition of cell proliferation and loss of the transformed phenotype (Figure 21). We concluded that intracellular immunization-based gene therapy strategies may emerge as a promising antiviral approach to interfere with the life cycle and tumorigenicity of HCV (Zemel 2004).
Figure 20
Figure 21
The antibodies
we isolated in that study were inhibitors of NS3 serine
protease activity. To isolated scFvs that are true
inhibitors, we sorted to a different screening strategy.
Figure 22
Figure 23
Our scFvs were further explored as potential intervention towards the eradication of HCV infection using advanced HCV RNA replicons. The potential of the NS3-neutralizing scFvs to suppress HCV RNA replication was evaluated using SEAP secreting replicon-bearing Huh7 cells. SEAP secretion was suppressed in replicon cells transiently transfected with NS3-neutralizing but not in a control cell line nor by control scFvs (Figure 24). This indicates that the effect is due to specific suppression of the HCV RNA replication by the NS3-neutralizing scFvs. These inhibitors suppress the replication of drug resistant mutants replicons as well, emphasizing their advantage over small molecule inhibitors. These Single-chain antibodies may emerge as useful clinical reagents as more specific and boadly administrated for the treatment of infectious diseases and cancer.
Figure 24
In addition to antibodies we turned to search for peptide aptamers as NS3 inhibitors. Peptide aptamers are short (in our case 8 amino-acid long) peptides that are stabilized by their presentation on a stable protein scaffold. We had initiated a study where a novel high-throughput in vivo genetic screen for NS3 catalysis and its inhibition was applied for inhibitors isolation. Here the screen was based on the concerted co-expression of a the reporter gene, recombinant NS3 and stabilized candidate molecules (MBP-scFvs and peptide aptamers) in E. coli. The peptide-aptamers were isolated from libraries where random sequences were inserted at the C-terminus of the E. coli MBP as linear peptides. Here too, the initial identification of inhibitory peptide aptamers was based on the their expression in bacteria that express the enzyme-substrate combination as well, by the appearance of blue colonies (NS3 inhibited) on the background of colorless colonies (NS3 active) on X-gal indicative plates. The peptide-aptamer inhibitors were validated as NS3 binders and as in vitro inhibitors of catalysis as well. We are currently evaluating the aptamers using the RNA replicons described above.
Full Publications
1. Benhar, I. and Engelberg-Kulka, H. (1991). A procedure for amino acid sequencing in internal regions of proteins. Gene 103: 79-92.
2. Benhar, I., Miller, C. and Engelberg-Kulka, H. (1992). Frameshifting in the expression of the Escherichia coli trpR Gene. Mol. Microbiol. 6: 2777-2784.
3. Benhar, I. and Engelberg-Kulka, H. (1993). Frameshifting in the expression of the Escherichia coli trpR gene by the bypassing of a segment of its coding sequence. Cell 72: 121-130.
4. Benhar. I., Miller, C. and Engelberg-Kulka, H. (1993). Frameshifting in the expression of the Escherichia coli trpR gene is modulated by translation initiation. J. Bacteriol. 175: 3204-3207.
5. Engelberg-Kulka, H., Benhar, I. and Schoulaker-Schwarz, R. (1993). Translational Introns: an additional regulatory element in gene expression. Trends in Biol. Sci. 18: 294-296.
6. Benhar, I., Wang, Q-C., FitzGerald, D. and Pastan, I. (1994). Pseudomonas Exotoxin A Mutants: replacement of surface-exposed residues in domain III with cysteine residues that can be modified with Polyethylene glycol in a site specific manner. J. Biol. Chem. 269: 13398-13404.
7. Benhar, I., Brinkmann, U., Webber, K.O. and Pastan, I. (1994). Mutations in the CDR loops of a recombinant immunotoxin that reduce its sensitivity to chemical derivatization. Bioconjug. Chem. 5: 321-326.
8. Roscoe, D.M., Jung, S-h., Benhar, I., Pai, L., Lee, B.K. and Pastan, I. (1994). Primate antibody response to immunotoxin: serological and computer-aided analysis of epitopes on a truncated form of Pseudomonas exotoxin. Infect. Immun. 62: 5055-5065.
9. Benhar, I., Padlan, E.D., Jung, S-H., Lee, B. and Pastan, I. (1994). Rapid humanization of the Fv of monoclonal antibody B3 by using framework exchange of the recombinant immunotoxin B3(Fv)-PE38. Proc. Natl. Acad. Sci. (USA) 91: 12051-12055.
10. Benhar, I. and Pastan, I. (1994). Cloning, expression and characterization of the Fv fragment of the anticarbohydrate monoclonal antibodies B1 and B5 as single-chain immunotoxins. Protein Eng. 7:
11. Benhar, I. and Pastan, I. (1995). Characterization of B1(Fv)PE38 and B1(dsFv)PE38: single-chain and disulfide stabilized Fv immunotoxins with increased activity that cause complete remissions of established human carcinoma xenografts in nude mice. Clin. Cancer Res. 1: 1023-1029.
12. Benhar, I., Reiter, Y., Pai, L.H. and Pastan, I. (1995). Administration of disulfide-stabilized Fv-immunotoxins B1(dsFv)-PE38 and B3(dsFv)-PE38 by continuous infusion increases their efficacy in curing large tumor xenogragfts in nude mice. Int. J. Cancer 62: 351-355.
13. Li. M., Dyda, F., Benhar, I., Pastan, I. and Davies, D.R. (1995). The crystal structure of Pseudomonas aeruginosa exotoxin domain III. Proc. Natl. Acad. Sci. (USA) 92: 9308-9312.
14. Benhar, I. and Pastan I. (1995). Identification of residues that stabilize the single chain Fv of MAb B3. J. Biol. Chem. 270: 3373-3380.
15. Li. M., Dyda, F., Benhar, I., Pastan, I. and Davies, D.R. (1996). Crystal structure of the catalytic domain of Pseudomonas exotoxin A complexed with a nicotinamide adenine dinucleotide analog: implications for the activation process and for ADP ribosylation. Proc. Natl. Acad. Sci. (USA) 93: 6902-6906.
16. Scherf, U., Benhar, I., Webber, K.O.W., Pastan, I. and Brinkmann, U. (1996) Cytotoxic and Antitumor activity of a Recombinant Tumor Necrosis Factor-B1(Fv) Fusion Protein on LeY-antigen expressing Human Cancer Cells. Int. J. Cancer 2: 1523-1531.
17. Almog, O., Benhar, I., Vasmatzis, G., Tordova, M., Lee, B., Pastan, I. and Gilliland, G.L. (1998) Crystal structure of the disulfide-stabilized Fv fragment of anticancer antibody B1: conformational influence of an engineered disulfide bond. Proteins 31: 128-138.
18. Berdichevsky, Y., Ben-Zeev, E., Lamed, R. and Benhar, I. (1999). Phage display of a cellulose binding domain from Clostridium thermocellum and its application as a tool for antibody engineering. J. Immunol. Methods 228: 151-162.
19. Berdichevsky, Y., Lamed, R., Frenkel, D., Gophna, U., Bayer, E., Yaron, S., Shoham, Y. and Benhar, I. (1999) Matrix-assisted refolding of single-chain Fv-cellulose binding domain fusion proteins. Protein Express. Purif. 17: 249-259.
20. Frenkel, D., Solomon, B. and Benhar, I. (1999). Modulation of Alzheimer's beta amyloid neurotoxicity by an anti-aggregating single-chain antibody. J. Neuroimmunology, 106: 23-31.
21. Benhar, I., Nahary, L., Shaky, S., Azriel, R., Berdichevsky, Y., Tamarkin, A. and Wels, W. (2000) Highly efficient selection of phage antibodies mediated by display of antigen as Lpp-OmpA' fusions on live bacteria. J. Mol. Biol. 301: 893-904.
22. Benhar,I. (2001) Biotechnological Applications of Phage and Cell Display. Biotechnology Advances. 19: 1-33.
23. Zemel, R.,Gerechet, S., Greif, H., Bachmatove, L., Birk, Y., Golan-Goldehirsh, A., Berdichevsky, Y., Benhar, I., and Tur-Kaspa, R. (2201). Cell transformation induced by Hepatitis C virus NS3 serine-protease. J. Viral. Hepatitis, 8:96-102.
24. Bach, H., Mazor, Y., Shaky, S., Shoham-Lev, A., Berdichevsky, Y., Gutnik, D.L. and Benhar, I. (2001). E. coli maltose-binding protein as a molecular chaperone for intracellular antibodies. J. Mol. Biol. 312:79-93.
25. Benhar, I., Eshkenazi, I., Neufeld, J. Opatowsky, J., Shaky, S. and Rishpon, J. (2001). Phage displaying a recombinant single-chain antibody in electrochemical detection of the pathogenic bacterium Listeria monocytogenes. Talanta. 55: 899-907.
26. Mazor, Y., Gilad, S., Benhar, I. And Gazit, E. (2002). Identification and chracacterization of a novel molecular recognition and self-assembly domain within the islet amyloid polypeptide. J. Mol. Biol. 322: 1013-1024.
27. Berdichevsky, Y., Zemel, R., Bachmatov, L., Abromovich, A., Koren, R., Golan-Goldhirsh, A., Tur-Kaspa, R. and Benhar, I. (2003). A novel high throughput screening assay for HCV NS3 serine protease inhibitors. J. Virol. Methods 107: 245-255.
28. Denkberg, G., Lev, A., Eisenbach, L., Benhar, I. and Reiter, Y. (2003) Selective targeting of melanoma and APCs using a recombinant antibody with TCR-like specificity directed toward a melanoma differentiation antigen. J. Immunol. 171: 2197-2207.
29. Azriel-Rosenfeld, R., Valensi, M. and Benhar, I. (2004) A human synthetic combinatorial library of arrayable single-chain antibodies based on shuffling in vivo formed CDRs into general framework regions. J. Mol. Biol. 335(1):177-192.
30. Haus-Cohen, M., Assaraf, Y., Binyamin, L., Benhar, I. and Reiter, Y. (2004) Disruption of p-glycoprotein anticancer drug efflux activityby a small recombinant single-chain fv antibody fragment targeted to an extracellular epitope. Int. J. Cancer 109: 750-758.
31. Zemel, R., Berdichevsky, Y., Bachmatov, L., Benhar, I. and Tur-Kaspa, R. (2004) Inhibition of Hepatitis C Virus NS3-mediated cell transformation by recombinant intracellular antibodies. J. Hepatol. 40: 1000-1007.
32. Zilberman-Peled, B., Benhar, I., Coon, S. L., Ron, B., Gothilf, Y. (2004) Duality of serotonin-N-acetyltransferase in the gilthead seabream (Sparus aurata): molecular cloning and characterization of recombinant enzymes. Gen. Comp. Endocrinol. 138: 139-147.
33. Mazor, Y., Keydar, I., Benhar, I. (2005) Humanization and epitope mapping of the H23 anti-MUC1 monoclonal antibody reveals a dual epitope specificity. Mol. Immunol. 42: 55-69.
34. Gal-Tanamy, M., Zemel, R., Berdichevsky, Y., Bachmatov, L., Tur-Kaspa, R. and Benhar, I. (2004). HCV NS3 Serine Protease-Neutralizing Single-Chain Antibodies Isolated By a Novel Genetic Screen. J. Mol. Biol. 347: 991–1003.
35. Ofir, K., Berdichevsky, Y. Benhar, I., Azriel-Rosenfeld, R., Lamed, R., Barak, Y., Bayer, E. A. and Morag, E. (2005) Versatile protein microarray based on carbohydrate-binding molecules. Proteomics 5:1806-1814.
36. Shaki-Loewenstein,
S., Zfania, R., Hyland, S., Wels, W. S. and Benhar,
I. (2005) A universal solution for stable intracellular
antibodies. J. Immunol. Methods 303: 19-39.
37. Yacoby I, Shamis M, Bar H, Shabat D, Benhar I. (2006) Targeting antibacterial agents by using drug-carrying filamentous bacteriophages. Antimicrob Agents Chemother. 50(6):2087-97.
38. Artzy Schnirman, A., Zahavi, E., Yeger, H.,, Rosenfeld, R., Benhar, I., Reiter1, Y. and Sivan, U. (2006) Antibody Molecules Discriminate Between Crystalline Facets of Gallium Arsenide semiconductor. Nano Lett. 6: 1870-1874.
39. Rubinstein, D.B., Karmely, M., Ziv, R., Benhar, I., Leitner, O., Baron, S., Katz, B.Z., Wreschner, D.H. (2006) MUC1/X protein immunization enhances cDNA immunization in generating anti-MUC1 alpha/beta junction antibodies that target malignant cells. Cancer Res. 66:11247-11253.
40. Machlenkin, A., Azriel-Rosenfeld, R., Volovitz, I., Vadai, E., Lev, A., Paz, A., Goldberger, O., Reiter, Y., Tzehoval, E., Benhar, I. and Eisenbach, L. (2007) Active immunization with PAP-3, a novel human prostate cancer peptide, inhibits carcinoma development in HLA transgenic mice. Cancer Immunol. Immunother. 56:217-226.
41. Mazor Y, Barnea I, Keydar I, Benhar I. (2007) Antibody internalization studied using a novel IgG binding toxin fusion. J. Immunol. Methods 321(1-2):41-59.
42. Yacoby I, Bar H, Benhar I. (2007) Targeted drug-carrying bacteriophages as antibacterial nanomedicines. Antimicrob. Agents Chemother. 51(6):2156-63.
43. Benhar I. (2007) Design of synthetic antibody libraries. Expert Opin. Biol. Ther. 7(5):763-79.
44. Mazor,
Y., Noy, R., Wels, W.S. and Benhar, I. (2007) chFRP5-ZZ-PE38,
a large IgG-toxin immunoconjugate outperforms the
corresponding smaller FRP5(Fv)-ETA immunotoxin in
eradicating ErbB2-expressing tumor xenografts. Cancer
Lett. 257(1):124-135.
1. Benhar I., Miller, C., and Engelberg-Kulka, H. (1990). Frameshifting in the expression of the trpR gene of Escherichia coli. In: McCarthy, J.E.G., and Tuite, M.F. (eds): Post Transcriptional Control of Gene Expression. Springer Verlag, Berlin, pp. 591-602.
2. Benhar I. and Pastan, I. (1997). Tumor Targeting by Antibody-Drug Conjugates. In: Harris, W.J. and Adair, J.R. (eds): Antibody Therpeutics. CRC Press, Boca Raton, pp. 73-85.
3. Benhar, I., Tamarkin, A., Marash, L., Berdichevsky, Y., Yaron, S., Shoham, Y., Lamed, R. and Bayer, E. A. (2001). Phage display of cellulose binding domains for biotechnological application. In Glycosyl Hydrolases for Biomass Conversion. ACS Symposium Series 769 (M. E. Himmel, J. O. Baker and J. N. Saddler, ed.), pp. 168-189. American Chemical Society, Washington, DC.
4. Benhhar, I. and Reiter Y. (2001). Phage display of single-chain antibodies (scFvs). Current Protocols in Immunology. Chapter 10.19B. John Colligan (Ed). John Wiley & Sons, Inc, USA.
5. Benhar, I. and Berdichevsky Y. (2002). Large Scale Production of Recombinant Antibodies By Utilizing Cellulose Binding Domains. In: Welschof, M. and Krauss, J. (eds): Methods in Molecular Biology Vol. 207; Recombinant Antibody Technology for Cancer Therapy. Humana Press Series. pp. 443-454.
6. Nahary,
L. and Benhar, I. (2009) Design of Human Synthetic
Combinatorial Library of Single-chain Antibodies.
In: Therapeutic Antibodies, Methods In Molecular Biology.
Humana Press 525:61-80.
1. Pastan, I., Benhar, I., Padlan, E. A., Jung, S-H. and Lee, B. (1999) Humanized B3 antibody fragments, fusion proteins, and uses thereof. US Patent Number 5889157. Issue date 30/3/1999.
2. Pastan, I. And Benhar, I. (1999) Chimeric and mutationally stabilized tumor-specific B1, B3 and B5 antibody fragments; immunotoxic fusion proteins; and uses thereof. US Patent Number 5981726. Issue Date 9/11/1999.


text:
Airline Merger: DOJ Wins Big at National, Loses on Connecting Routes
CHARLOTTE, N.C. (TheStreet) -- It's puzzling to hear all the pronouncements that the Justice Department failed to get anything in return for settling its lawsuit opposing the merger of US Airways (LCC) and American (AAMRQ) .
At Washington Reagan National Airport, the key battleground in the DOJ's struggle for merger concessions, the DOJ seems to have gotten nearly everything it wanted. On the 1,000 network connections that were at the heart of the complaint it filed in August, DOJ seems to have gotten very little. But there a solution, in the words of aviation consultant Bob Mann, "was all ephemeral stuff -- it's not even manageable."
Start with the assumptions that while the key for DOJ was to get every one of the slots used by American before the merger, US Airways didn't want to give up any slots at all, which is what CEO Doug Parker argued in appearances before Senate subcommittees reviewing the merger bid in Marchand in June.
Asked Tuesday during a media conference what was the toughest concession in the settlement package, Parker responded: "The most painful is the slots at Reagan."Today, US Airways operates 243 daily departures at National, while American operates 51. After the merger, the new American will operate 250 daily departures at National, according to a US Airways employee newsletter published Thursday. So if Justice asked for all of the American slots at Reagan, it got enough to enable 44 daily departures. Those slots can now turn over to competitors. It is assumed those competitors will exert downward pressure on the fares at National, especially because the Justice Department has specifically declared that carriers with low-fare models can bid for them, while Delta (DAL) and United (UAL) cannot. "It seems to me DOJ got a good settlement for a lawsuit that airline apologists said had no basis in fact," online travel commentator Joe Brancatelli tweeted. "Of course, no merger would have been better." The settlement "opens 138 slots for low-cost carriers at Reagan National and LaGuardia as well as gates at five other major airports, which the Justice Department views as transformational for consumers," said Jack Butler, a Skadden Arps partner who represents American's official creditors committee and has been involved in the merger and settlement effort.
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text:
ELECTRIC CASTLE is the first festival in Romania that took electronic dance music and live concert sounds on a castle's domain.
ELECTRIC CASTLE represents an idea, concept and event created and planned by BOILER EVENTS, the artistic force behind Boiler Club – Cluj-Napoca's clubbing scene breakthrough.
Banffy Castle, the place where the festival is held, is situated near Cluj-Napoca and it's the biggest castle in Transylvania. Formerly known as the Transylvanian Versailles, the Banffy castle of Bontida is one of the most beautiful historic buildings in the Carpathian basin.
24 acres, 6 buildings: castle, stables, chapel, flour mill. The castle saw them all and in it's hundred of years filled with history it went from former aristocratic residence to military hospital, drivers' school, children hospital and historical monument under the Royal House's auspice.
Alongside BOOHA COLLEGE, BOOHA BAR, AMERICAN EXPERIENCE we will make sure that this innovative music festival will shake up the way people interact with music.


text:
Indonesia expects to pay around U.S. $800M for their new subs. The Australian Defence Establishment wants us to believe that they can make 12 replacements for the Collins class for $36B. Given the current ship-building debacle, we should act with extreme caution for any warship wants by Defence.
There are those that still hold on to the tired old claim that a replacement for the Collins sub needs to be big, custom, long range (or at least longer range than any off-the-shelf sub to support their case) and have a variety of features. Features that are "Australian". Just about any defence product Australia buys will have some "Australianization" to include our current Super Hornets (some navigation aids of no particular concern). Yet, how much is too much? Answer; the Air Warfare Destroyer project, which is only kind of like the Spanish design the public was mislead into believing was low risk.
Those that want the super unobtainable homemade and gold-plated submarine are running a con. They don't have the skills to back up their claims and they will do and say anything to make sure there is enough backing for this jobs program. The number-one goal is for it to be a jobs program since they don't have a clue what the design will cost; how long it takes to build; how much it will cost to sustain, or; if it will even work properly. They do not have a CV that deserves our confidence. This camp even seems to prefer that it is their plan or nothing; even if their road map has a good chance of delivering low or no worthwhile combat capability.
So how could Australia fund some gap-fill off-the-shelf subs until (if) home industry gets their act together? Easy. There is $3.2B of unspent commitment sitting out there in fantasy land for 14 F-35 Joint Strike Fighters; AKA, "Faulkner's Folly". As the F-35 is a fearfully high risk program, the government should hand over no money for this purchase. Funds should go toward new off-the-shelf subs.
In any event, all of this depends on a failed experiment known as the Defence Material Organisation (DMO) to manage everything. Which means, any low risk project becomes medium risk; any medium risk project becomes high risk and any high risk project—like 12 home grown subs for $36B (or the F-35)—becomes feeble minded fantasy.
We took Defence and industry on their word before with the Air Warfare Destroyer which is a much less complex project than building subs. We should never do that again. Incredible claims of ship-building skills and project management for submarines needs incredible proof. We should give the Defence Establishment a chance to come up with a properly risk-assessed design, but they should not be allowed to hold our national defence hostage.
Tuesday, June 7, 2011
Don't let the Defence Establishment hold Australia's tax dollars hostage over subs
3 comments:
That's a great post. Well said.
- June 8, 2011 at 9:50 AM
- ELP said...
Yeah. From the massive amount of comments, it was like throwing a dead cat in the room.
- June 8, 2011 at 11:27 PM
There is also a great deal of silence from the Press in Australia.
FOr something so fundamnetally wrong, ie DMOD etc it is amazing
- June 9, 2011 at 8:24 AM
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Reviewed by: craftymom2012
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Title: great look but itchy!
Date: Jun 30, 2014
We used these only for the men at the party and they looked great in photos (much nicer than the cheap plastic ones or even the flower leis). But they were super itchy around the neck. It was worth it for the photos but the guys didnt keep them on long. :(
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Confess! Whats the craziest force of habit you've done in the "Real World" - page 16
I have heard some of the craziest things nurses have done out of force of habit in the "Real World". Aka out of the hospital. I've heard that some sign their checks with first initial, last name RN. Someone ran to aisle... Read More
- 1Feb 23, '11 by LaterAlligatorI'm an aide in a dementia unit, and I have one resident that is like a grandfather; we all call him Pops, and he's so sweet. The other night after work when my husband told me goodnight, I must have been really tired, because without thinking I said "Goodnight Pops, I'll see you tomorrow"! Confused my husband, that's for sure!
- 0Feb 24, '11 by LUV ALZI work with MD pt's who are all ventilator dependent and some are on pulse ox monitors at night. I have been known to be at home and wake form a dead sleep and bolt to the kitchen to answer an alarm, usually the microwave or the oven timer. I always wash my hands before and after I use the restroom. When I need something from my purse I usually start out by doing a full body pat down from the chest to my thighs because I am used to everything being in my scrub pockets. I am forever reaching for my lanyard that has my hand sanitizer on it. I answer my phone with the official company response. When the big guy gets out of the shower I find my self trying to do a quick head to toe skin assessment.
- 0Apr 22, '11 by FranEMTnurseI have facial hair that I often shave off. My caregiver placed some VO5 hair conditioner in my hand to rub into my hair, but without thinking, I rubbed it all over my face turning it blue. My caregiver's eyes got big and she had such a funny puzzled look on her face. That was when I realized what I did. Ha ha, I told her I was trying to condition my facial hairs.
- 10Aug 16, '11 by SnowShoeRNI got another one.
The other morning (after working a hard shift overnight where we had a couple of rapid responses) I was waiting for my bus and checking the news and weather on my phone. My heart started to race when I saw "75/57."
Omigod! Whose pressure is dropping!? Is it room 43 or room 57!?
Until I realized it was the forecast for the high and low temperatures for the day.
- 2Aug 16, '11 by Poi DogI approach elderly people and ask if they need help with anything.Last edit by Poi Dog on Aug 16, '11
- 2Aug 16, '11 by APatriganiRNhaha! I'm a new grad and I accidentally signed RN on credit card receipt the other day...I feel like much less of a d-bag now that I see I'm not the only one who's done that! ;-)
Also, can't walk by a Purell dispenser without taking some haha
- 2Aug 16, '11 by Poi DogQuote from APatriganiRNThat is true for me toohaha! I'm a new grad and I accidentally signed RN on credit card receipt the other day...I feel like much less of a d-bag now that I see I'm not the only one who's done that! ;-)
Also, can't walk by a Purell dispenser without taking some haha
- 0Aug 16, '11 by sweetsleep28I've asked the boyfriend in my sleep to roll over so I can check your bottom.
I've also scared the living bejezus of out of him when he has woken up to me hovering over him to see if he is breathing.
I'm also not alot to talk about work related things because I get too graphic.
Found myself trying to turn on my laptop by pressing my pointer finger to the mouse pad like I do to get into the med dispense at work.
While on vacation this summer with my boyfriends family any ailment that could have possibly come up I had a cure for. heartburn, sun burn, allergies acting up, cut you finger chopping onions? I can help you out with that!
I also kept track of how many times my friends have voided while were are out for the night.
Yes I am nurse, no I'm a doctor you should probably get that checked out.
Guilty as well of signing checks with LPN after my name.
- 0Aug 19, '11 by NewAggieGrad09Telling time in the 2400 format. Anytime I'm watching a military show, I get so excited because I understand what they mean by "We will start running at 1400 hours". SMH


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Your Guide to Makeup: Latina Edition
Celebrity makeup artist Mally Roncal breaks down what you need to know about choosing the right foundation, powder, and eye shadow for your skin tone!
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Dear Valued Customers and New Ones from Sidney,
LIFESCAPES FLORIST, formerly of Otego has relocated its shop to Sidney. This move is a better opportunity for us to continue to grow our business, while still providing you with the very best floral creations in the industry. Our hope is to maintain your loyalty, while securing a better future for our business plan. We ask for your assistance in spreading the word to your friends, family, and colleagues.
updates, photos of our progress and important information
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Meanings of Flowers
Copyright 2009 - 2013 Lifescapes Florist


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The name of the cat, Mrs. Norris, in Harry Potter is author J.K. Rowling's nod to Jane Austen's influence in her writing.
She said, "I'm big on names – I like names, generally. You have to be really careful giving me your name if it's an unusual one, because you will turn up in book six. Erm – I – I collect – some of them are invented; Voldemort is an invented name, Malfoy is an invented name, Quidditch is invented, erm – but I also collect them, from all kinds of places: maps, street names, people I meet, old books, old saints, erm – Mrs Norris, people will have recognised, comes from Jane Austen."
She went on to explain that "Dumbledore is an old English word meaning bumblebee. Because Albus Dumbledore is very fond of music, I always imagined him as sort of humming to himself a lot."
Mrs. Norris is a character from Jane Austen's 1814 novel, Mansfield Park.
Source: accio-quote.org


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Real Temp 3.70
No installation necessary
There is no setup pack involved, so you can drop the program files in any part of the hard disk and just click the executable to launch the app. Another possibility is to save Real Temp to a USB flash disk or similar storage unit, in order to run it on any PC easily. Plus, it does not create new entries in the Windows registry or Start menu.
Clear-cut interface with live readings
The main window does not include visually appealing elements but it is simple to navigate. It shows the current processor temperature and difference from maximum value, minimum and maximum reading recorded and time of recording, along with the thermal status.
Run sensor benchmarks and customize rich settings
It is possible to perform sensor tests to find out the average CPU load and difference from the maximum temp, time, speed and score. As far as customization settings are concerned, you can calibrate the maximum temperature, enable system tray icons for monitoring the CPU cores and maximum temp, enable alarms, log all activity to file, and more. Settings may be restored to default.
Evaluation and conclusion
Real Temp has minimal impact on system resources and a great response time. It shows accurate data and worked well in our tests, without triggering the OS to hang, crash or pop up error messages. Thanks to its advanced options and versatile range of customization settings, Real Temp should meet the requirements of most users looking for a straightforward tool for monitoring processor temperature. Too bad it does not come equipped with automated tasks in case of overheating, such as PC shutdown.
Reviewed by Elena Opris, last updated on July 16th, 2014
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- Support for Sandy Bridge CPUs.
- VID based power consumption estimates for the newer CPUs.
- Improved log file formatting.
- Added a single system tray icon that reports the maximum core temperature.
Application descriptionReal Temp is an application created to monitor your Processor's temperature. Each core on these processors has a digit...


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- Sheepshead Bites - http://www.sheepsheadbites.com -
Brooklyn's Largest Toys For Tots Drive Is Back!
Posted By Willie Simpson On December 6, 2012 @ 2:15 pm In Events | 1 Comment
Councilman Lew Fidler, the 41st Assembly Democrats and Toy Drive founder Bryan Lee are back [2] with their 12th annual 'Toys For Tots' toy drive. The annual toy drive is throwing their Grand Finale Party on Thursday night, December 20 from 7:30 to 9:30 p.m. at the 41st A.D. Dems Club, 2952 Ave. R.
The toy drive is currently accepting donations, and if you wish to give in person, you can bring a new, unwrapped toy to the party. Donations are also accepted by mailing a check payable to Community First Toy Drive- 41st A.D. Dems Club c/o Bryan Lee, Executive Director 2664 Dorothy Street Bellmore, NY 11710.
Special guests include Mr. Met, famed New York Mets mascot, making his 11th straight appearance and Sandy the Seagull of the Brooklyn Cyclones flying in for his fourth straight time.
You can also attend and donate to one of the Satellite Toy Drive Sponsors such as the 4th Be Proud Foundation Toy Drive at the Cherry Hill Gourmet Market located at 1901 Emmons Avenue held on Monday, December 17, between 6 and 8 p.m., or through Community Board 15 [3].
Article printed from Sheepshead Bites: http://www.sheepsheadbites.com
URL to article: http://www.sheepsheadbites.com/2012/12/brooklyns-largest-toys-for-tots-drive-is-back/
URLs in this post:
[1] Image: http://www.sheepsheadbites.com/?attachment_id=40182
[2] back: http://www.sheepsheadbites.com/2011/12/fidler-and-41st-assembly-dems-hoping-for-biggest-toy-drive-ever/
[3] through Community Board 15: http://www.sheepsheadbites.com/2012/12/bullet-points-community-board-15-november-27-2012/
[4] Toys For Tots Month Continues Tonight At Cherry Hill Gourmet Market: http://www.sheepsheadbites.com/2012/12/toys-for-tots-month-continues-tonight-at-cherry-hill-gourmet-market/
[5] Teens Cross Cultural Lines To Deliver Toys For Tots: http://www.sheepsheadbites.com/2012/12/teens-cross-cultural-lines-to-deliver-toys-for-tots/
[6] Toy Drive In Full Swing, As Local Branch Nets 500 Toys: http://www.sheepsheadbites.com/2011/12/toy-drive-in-full-swing-as-local-branch-nets-500-toys/
[7] Southern Brooklyn Toy For Tots Drive Breaks All Records… Again!: http://www.sheepsheadbites.com/2012/12/southern-brooklyn-toy-for-tots-drive-breaks-all-records-again/
Click here to print.
Copyright © 2008-2011 Sheepshead Bites. All rights reserved.


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Using Buttons
Buttons are relatively basic pieces of logic, as standing on one will activate another piece of logic (Like an Emitter). However, the button can be "fooled" by placing a block of material on the button so it always gives off an "On" signal.


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Sounds like a good idea. I am up for it if others are as well.
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Hello and welcome to the web site for Suffolk Kennel Association. We are a ring craft and socialisation club and we aim to cover all aspects of training a dog for the show ring.
We have a dedicated team of trainers who between them show breeds from all 7 groups and have many many years of experience in both showing and judging .
We hold training classes on a Tuesday evening at Hintlesham & Chattisham Community Centre IP8 3PS from 7.30pm - 10.00pm and we hold a limit show in Febuary and an open show in August each year, we also hold K.C. teaching seminars on a regular basis please check our shows & events page for up to date information on all forthcoming events.
If you are unable to find the information you need on this site please feel free to contact us by email or phone and we will do our best to answer all your enquiries.
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With its prize-winning, richly symbolic architecture, the War Museum, located near downtown Ottawa, provides a spectacular setting for exhibitions, public programs and other events that illustrate the way in which human conflict, in Canada and around the globe, has changed the face of the world.
If you're interested in our beautiful National Capital generally, or are thinking about visiting, check out our hotel packages and the Web sites describing the region.
Sources of Tourism Information
Read reviews of the Canadian War Museum on TripAdvisor.
- Regional Map
- Ottawa Tourism
- Tourisme Outaouais
- Capital's Infocentre
- City of Ottawa
- City of Gatineau Tourism
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Monday, Feb. 23
ITM Workshops. Workshops on Excel: Charting and D2L: HTML Editor will be presented. For detailed information or to register, visit the ITM Workshop Web site.
Women's Basketball. The Blugolds will host the Wisconsin Intercollegiate Athletic Conference quarterfinal game. Opponent to be announced. Tickets, available in advance at the Service Center, are $7 for reserved seats, $6 for general admission or $3 for UW-Eau Claire students and children 5 through 18. Passes will not be honored. Season ticket holders and All Sports pass patrons have until noon today to have a reserved seat ticket guaranteed for purchase. Zorn Arena, 7 p.m.
Tuesday, Feb. 24
Guest Lecture. Carlos Reboratti, University of Buenos Aires, Argentina, and Tinker visiting professor at UW-Madison, will present "Socio-political Crisis and Recovery in Argentina." Sponsored by the economics department, Latin American studies program, Academic Affairs and the Professional Development Program. Presidents Room, Davies Center, 4 to 5 p.m. Reception to follow .
Program. Featuring "Wonders of Deep
Space," a show about how we learned that galaxies exist, how stars
are born and how their lives end. The show is aimed at adults and children
10 and older. Tickets are $1 and must be purchased in advance from the
Service Center. L.E. Phillips Planetarium, 7 p.m.
Gerontology Lecture. Jennifer Johs-Artisensi, allied health professions, and Carrie Nelson, a physician at Red Cedar Medical Center in Menomonie, will present "Depression in the Older Person." For more information, contact Meredith Wolf at email@example.com or 836-3543. Marshfield Clinic-Oakwood Center, Eau Claire, noon to 1 p.m. Full story.
Faculty/Academic Staff Forum. Zoltan Grossman, geography and anthropology/American Indian studies, will present "Unlikely Alliances: Treaty Conflicts and Environmental Cooperation Between Native American and Rural White Communities." Presidents Room, Davies Center, 12:10 to 12:50 p.m.
Artists Series. The Princely Players, an eight-member vocal ensemble from Nashville, Tenn., will present "On the Road to Glory." The performance will feature such songs as "Amazing Grace," "Steal Away," "Swing Low, Sweet Chariot" and "We Shall Overcome" that galvanized the freedom struggle of Africans in America. Tickets, available at the Service Center and at the door, are $15 for the public, $13 for those 62 and older and UW System/CVTC faculty/staff, or $5 for those 17 and younger and UW System/CVTC students. Schofield Auditorium, 7:30 p.m. Full story.
Jazz at Night. University musicians present the best in jazz. The Cabin, Davies Center, 8 p.m.
Feb. 26
ITM Workshops. Workshops on Introduction to D2L, Excel Formulas: Level 2 (6 of 7) and Planning for Effective Quantitative Research will be presented. For detailed information or to register, visit the ITM Workshop Web site.
Student Recital. Featuring Dawn Holte, euphonium. Phillips Recital Hall, Haas Fine Arts Center, 5 p.m.
Campus Films. "8 Women." (France and Italy 2002, 111 min., directed by François Ozon, in French, subtitled, rated R.) The patriarch of a dysfunctional family is found stabbed to death — and the eight women in his life are all suspects. Set in a snowbound cottage in the 1950s, this candy-colored murder mystery stars the top cinema sirens of France. Sponsored by the International Film Society. Tickets are $2 at the Service Center for IFS members and UW-Eau Claire faculty/staff or $1 for UW-Eau Claire students. Davies Theatre, 6 and 8:30 p.m. Through Feb. 29.
University Theatre. Featuring the musical "Cabaret," directed by Richard Nimke; choreographed by Toni Poll-Sorensen; and conducted by Mitra Sadeghpour. "Cabaret" depicts the decadence and apathy that paved the way for the Third Reich in pre-World War II Germany. Life in the sleazy Kit Kat Klub and on the streets of Berlin act as a metaphor and a commentary on political and social responsibility. The production is not intended for young audiences. Riverside Theatre, Haas Fine Arts Center, 7:30 p.m. Through Feb. 28 and March 2-6 at 7:30 p.m., and Feb. 29 and March 7 at 1:30 p.m. Full story.
Feb. 27
Men's Tennis. Alumni match at McPhee, 4 p.m.
Campus Films. "8 Women." Davies Theatre, 6 and 8:30 p.m. See Feb. 26 item.
University Theatre. "Cabaret." Riverside Theatre, Haas Fine Arts Center, 7:30 p.m. See Feb. 26 item.
UAC Cabin. Featuring Eau Claire native Matt Wahl. Wahl's deep baritone voice and upbeat, insightful songs have won him fans throughout the Midwest. The Cabin, Davies Center, 8 p.m.
Feb. 28
Women in the Outdoors Event. Women are invited to this event to learn basic rock climbing, fly fishing, archery and cross country skiing. Cost is $15 per person. For more information, contact Dan Langlois at email@example.com or 836-3616. Outdoor Recreation, Hilltop Center, 8 a.m. to 4:30 p.m.
Men's Tennis. College of St. Scholastica at McPhee, 10 a.m.
Campus Films. "8 Women." Davies Theatre, 6 and 8:30 p.m. See Feb. 26 item.
University Theatre. "Cabaret." Riverside Theatre, Haas Fine Arts Center, 7:30 p.m. See Feb. 26 item.
UAC Cabin. Featuring Me and Neil, a local folk-rock band. The Cabin, Davies Center, 8 p.m.
Sunday, Feb. 29
Campus Films. "8 Women." Davies Theatre, 6 and 8:30 p.m. See Feb. 26 item.
Monday, March 1
Foster Gallery Exhibit. See Feb. 23 item.
University Bulletin • Vol. 51, No. 21 • Fifth Week • Spring Semester • Feb. 23, 2004
Liz Wolf Green, Editor, UW-Eau Claire News Bureau, Schofield 201, (715) 836-4741
Diane Walkoff, Editorial Assistant. Updated: February 23, 2004


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One of the comments made by a panelist at the "Peace Corps and Africa" conference I attended in Madison, Wisconsin, in late March keeps reverberating in my mind. At the Oral History session, Bob Klein (Ghana '61-63), creator of the Archival Project (email@example.com), said, "The best way to empty the room at a family gathering is to begin telling your Peace Corps stories."
People laughed – as if this were a joke. In fact, it struck me as an epiphany. Ah, yes, I thought, this is precisely why literary agents, book editors and commercial publishers steer way clear of Peace Corps memoirs. They know such predictable (read: "boring") accounts of do-goodism (no guns, no gore) in places most Americans have never even heard of will cause prospective book buyers – even friends and family members — to empty the room.
But what if one's true Peace Corps story included a rape? That would hold people's attention, to be sure. Sadly, in our society, sex and violence (put them together and you have rape) sells.
This appears to be what the ratings-conscious media and some DC lawmakers have been focusing on this year of the Peace Corps' Golden Anniversary: Not the quiet good that has been done by the agency and the roughly 200,000 people who have served as Peace Corps volunteers in far-off lands over the past fifty years, but rather the incidents of rape and sexual assault of young female PCVs by host country nationals.
Please don't misunderstand me. I am not at all minimizing these horrendous and traumatic acts of violence. My heart goes out to the brave victims who have come forward and to all victims of this unspeakable crime everywhere it occurs. I applaud all efforts to improve the training and safety of Peace Corps volunteers, and I am sure such changes will be made immediately.
But I would also like to see this currently "hot" Peace Corps story put into perspective. I would like to see a segment on "20/20" (similar to the one aired last January that kicked off this 50th anniversary year with a piece on rapes in the Peace Corps) — as well as Congressional hearings in Washington — on the subject of rapes in the U.S. military. I suspect that the numbers that emerge from this investigation might even be higher.
Rape, tragically, happens everywhere in this world, wherever women (and young girls and even men and boys) are vulnerable to angry, aggressive, violent men – whether it's an expensive hotel room in New York, a U.S. military base in Afghanistan, an American college dorm room, or even the seeming "safety" of home in Anytown, U.S.A. Who is to blame? What is the solution? Unfortunately, there are no easy answers.
When I lived on the Upper West Side of Manhattan near Columbia University for twenty years (before joining the Peace Corps) and jogged in Riverside Park every morning, I carried a set of keys in my right hand, with the points of the keys sticking out of my fist. Fortunately, I never had to defend myself this way. But I'm convinced that being prepared made me appear less vulnerable to possible attackers.
Right now, it seems to me, the Peace Corps itself is under attack. At a time when we should be celebrating its achievements, we're hearing, reading, and seeing only horror stories. Why? What's really behind this, I wonder? It smells politically fishy to me.
Since this is meant to be a food-related blog, I'll try to draw a culinary analogy now: A good, careful, thoughtful cook doesn't buy smelly fish. Similarly, I don't "buy" that this current media frenzy over rapes in the Peace Corps is only about rapes in the Peace Corps. I suspect a hidden agenda among some to undermine and malign the Peace Corps, perhaps militarize it, or do away with it all together.
Among the maxims I have taped to the face of my desktop computer is this: "Silence is Acquiescence." We who have served in the Peace Corps and have positive stories to tell hold the keys in our hand. We can't be silent. We have a responsibility to tell our truths, too, even at the risk of emptying the room.


text: …by teaching them to honor the military instead of avoid the military. The book is titled The Night Santa Got Lost: How NORAD Saved Christmas. It turns out that NORAD (the North American Aerospace Defense) has tracked Santa every Christmas Eve since 1955 as he delivered toys. If only the military just played games like this instead of bombed and killed people.4:17 pm on November 26, 2012 Email Laurence M. Vance


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Police: Girl, 2, escapes dad's car, is struck, killed
Maryland State Police said a toddler has died after a weekend crash in Walkersville, Frederick County.
Troopers said the 2-year-old girl was struck by a car in the parking lot of Heritage Farm Park on Saturday afternoon.
Investigators said they believe the toddler managed to get out of her father's car on her own before her dad did. She then ran away from his car and was struck by another driver.
So far, no charges have been filed in the case.
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Psychology Dept
Kim Gingerich Brenneman, graduate of the EMC class of 1983, began her teaching career at Eastern Mennonite College in the fall of 1989. She received her Ph.D. in the spring of 2001 in Developmental and Educational Psychology. Her dissertation was titled The Well-being of Internationally Mobile Adolescents. She continues her interest in the well-being of Third Culture Kids.
Currently, Brenneman is conducting research on the relationship between cross-cultural experiential learning, hopeful thinking, and multicultural adaptation.
Brenneman is continuing to research the application of positive psychological principles to education, particularly in the development of hopeful thinking. Direct application of these principles is in the development of courses, assignments and seminars that integrate these, as well as other positive psychological concepts.
Kim has led three groups of EMU college students in semester-long cross-culturals to India in 2007, 2009, and 2011 and believes this is a positive life-changing experience for all involved!
Kim is married and has two children. She is an active member of Weavers Mennonite Church in Harrisonburg, VA.
Jess is an Assistant Professor of Psychology at EMU and also serves as faculty for the MA in Biomedicine program. Her research centers around executive function abilities. Executive function (EF) refers to higher order cognitive abilities necessary to consciously persist in a task or behavior despite facing challenges in the form of competing rules, distractions, or delays. A significant body of work has documented the relation between EF deficits and early child development. Low levels of EF are associated with clinical-level ADHD symptamotology (Gewirtz, Stanton-Chapman & Reeve, 2009), social difficulties (Fahie & Symons, 2003), academic difficulties (Zhou, Main, & Wang, 2010), and difficulty regulating emotions (Kochanska, Murray, & Harlan, 2000). Jess' research focuses on understanding the nature of EF and the factors associated with optimal regulatory abilities through a longitudinal, latent analysis framework. Her 2013 paper in Developmental Psychobiology ('Infancy predictors of preschool and post-kindergarten executive function') is one of their top 10 most downloaded articles and was a finalist for the 2013-2014 Hennessy-Smotherman-Wiley Best Student Paper Award.
Jess is an avid runner and dog lover. If you frequent downtown Harrisonburg regularly, you'll probably see her running with her dog Jake at some point.
Judy Mullet received her Ph.D. from Kent State University, where her dissertation work focused on context-motivated, conflict strategy choices of middle school students with learning disabilities. She was a school psychologist before coming to EMU, and has authored and co-authored recent publications on restorative discipline and harmful teacher behaviors. A member of the faculty since 1986, Dr. Mullet specializes in restorative discipline in schools, conducting workshops across the USA.


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Rent One of Our Privately Owned
Wells Maine Vacation Cottages
We will begin taking reservations for the 2015 season on Jan. 2, 2015
The Cottages at Summer Village is a 65 acre,
three-season resort community situated on the southern coast of Maine. Vacationers of all ages can enjoy a variety of activities or just relax in the peace and quiet of our natural setting. Our privately-owned one and two bedroom rental cottages come fully equipped with everything you need for a perfect vacation. High ceilings, extra large windows, and a three-season porch make each individually decorated cottage a gracious airy retreat.
When you rent one of our smoke-free cottages, you gain access to all the wonderful amenities at Summer Village, including:
- three swimming pools & two kiddie pools
- a fitness center
- Friday evening movies and popcorn
- 1.8 mile perimeter road great for easy walks and bicycling
- Wells/Ogunquit/Kennebunk Shoreline Trolley stop in front providing quick and easy access to local beaches
- A Summer Village Trolley that runs continuously, in season, around perimeter
- WiFi in Barn
- Game room with ping-pong, air hockey, video games, and more
- Tennis courts
- Playgrounds
- Computer room with printer and WiFi
If you feel like venturing from your pool chair or your three-season porch, the beaches, shops and restaurants of Wells, Ogunquit and the Kennebunks are just minutes away.
Take a look at the cottages available for rent and then book your cottage today for a vacation that you'll remember forever!
Our Blog
2015 Rental Season
Monday, December 15, 2014
Greetings from Wells Maine! Only 10 days until Christmas, oh dear! Aside from our holiday Read the Rest…
Children's Activities
Monday, February 24, 2014
It may seem a bit early to be thinking about vacation plans, however, we thought Read the Rest…
Ok Old Man Winter-Enough!
Saturday, February 15, 2014
Well, here we go again……..we are expecting about another 6-8 inches of snow this evening Read the Rest…
Contact Us
The Cottages At Summer Village
454 Post Road (Route 1)
Wells, Maine 04090
Mailing Address
The Cottages at Summer Village
PO Box 394,
Moody, ME 04054


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Kruisherenhotel, Maastricht $$$$
Tel: 31 43 329 20 20
From the editors of Condé Nast Traveler:The Kruisherenhotel, whose 60 rooms are in a former fifteenth-century church and cloister, caters to a highbrow crowd drawn to Maastricht for its burgeoning contemporary arts scene. The reception area and wine bar occupy the original nave and apse, while the open-walled breakfast room and library fill a newer mezzanine. Rooms are a mix of ancient and avant-garde, with oak parquet floors, Artemide lamps, chaise longues, and, in some, showy works of modern art. There are drawbacks, however: The service can be in-your-face snotty, and they nick you $28 for breakfast and $30 a day for parking. These irritations aside, the Kruisherenhotel is a good time. And if you can afford it, breakfast is a dazzling experience: The nave is filled with light from stained glass windows. 2006 Hot List
When to go: Spring for the flowers, summer for the city's cafés, and fall for the new gallery exhibitions.
Which room to book: A corner double such as No. 114, which has lots of light and a large original stained glass window; avoid the dark attic rooms (doubles, $351–$436).
Amenities: 24-hour Room Service, Bar/lounge
View the entire Hot List of the travel world's most noteworthy debuts in 2006 ›Subscribe now to Condé Nast Traveler for just $1 an issue! ›
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Landry Vineyards: Lisa Spann & Company at the Blanc Du Bois Stomp Fest
Event Date:
Sat, 07/19/2014
Event Time - From/To:
4:30 pm - 8:30 pm
Landry Vineyards winery outdoor concert featuring Lisa Spann & Company. A mix of Classic Rock, Blues, Jazz and, 60's, 70's and 80's music will have you, family & friends dancing all night. Make sure you pack your lawn chair, blanket and picnic dinner and enjoy the outdoor show.
Live Music
Presenting Organization:
Landry's Vineyard
Price Range:
$5-$8 per person; children 12 and under free.