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Breakthrough Articles
'Breakthrough Articles' present high-impact studies answering long-standing questions in the field of nucleic acids research and/or opening up new areas and mechanistic hypotheses for investigation. These articles are chosen by the Editors on the recommendation of Editorial Board Members and Referees. Articles are accompanied by a brief synopsis explaining the findings of the paper and where they fit in the broader context of nucleic acids research. Breakthrough Articles replace the older 'Featured Articles', which have been discontinued. They represent the very best papers published at NAR.
Chromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure
Grand RS, Pichugina T, Gehlen LR, Jones MB, Tsai P, Allison JR, Martienssen R, O'Sullivan JM.
In this work, Grand, O'Sullivan and co-workers capture the structure of the Schizosaccharomyces pombe genome and the transcriptome at different stages of the cell cycle. The authors find that the interactions between genes correlates with their transcriptional activity at specific stages of the cell cycle. In silico modelling of the genome suggests that genes that are highly expressed at all stages of the cell cycle are located towards the centre of the nucleus. Conversely, genes that are differentially expressed tend to be located towards the periphery of the nucleus. Based on these findings the authors conclude that the S. pombe nucleus is spatially divided into functional sub-nuclear domains that correlate with gene activity.
Free Full Text
Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination
Ignarski M, Singh A, Swart EC, Arambasic M, Sandoval PY, Nowacki M.
CAF-1 protein is a histone chaperone that is involved in the deposition of nucleosomes on newly replicated DNA and in the regulation of gene expression. In addition, defects of CAF-1 have been detected in various types of cancers. This study identifies a new role for CAF-1, based on findings in a laboratory model organism, the ciliated protozoan, Paramecium tetraurelia. A CAF-1 homolog in this organism facilitates the process of RNA-mediated programming of genome rearrangements. It enables selection of parental small RNAs that shape the genome of progeny cells during development. The research opens up new directions for studies of RNA-mediated maintenance of genome stability, eukaryotic chromosomal rearrangements, and perhaps DNA repair. Free Full Text
Mechanism of retinoic acid-induced transcription: histone code, DNA oxidation and formation of chromatin loops
Zuchegna C, Aceto F, Bertoni A, Romano A, Perillo B, Laccetti P, Gottesman ME, Avvedimento EV, Porcellini A.
Many eukaryotic genes are activated as a result of the interaction between nuclear receptors, or 'NRs' (which binding a wide variety of small molecules, such as thyroid hormones, that are important for transcriptional activation), their ligands, and DNA promoter regions. There are many reports on the modifications of chromatin and accumulation of NR-induced mRNAs of specific genes , but to date a mechanism that links activated receptor to the transcription of specific genes is still unclear. This paper provides evidence that transcriptional activation by one particular type of NR (the retinoic acid, or 'RA' receptor) involved a sequential wave of histone methylation changes, DNA oxidation and corresponding recruitment of DNA repair enzymes, formation of localized chromatin loops, and finally activation of the polymerase complex, resulting in the generation of RA-induced mRNAs. These early transcription activation event has been named the "non-productive transcription cycle", and are believed to characterize the earliest stages of transcription induction by nuclear receptors. Free Full Text
Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli
Clarke JE, Kime L, Romero AD, McDowall KJ.
This paper indicates that a major factor in defining the transcriptional landscapes of E. coli, and probably the large number of organisms that contain orthologues of RNase E, is 'direct entry' cleavage. It dispels the notion that the only common mechanism by which RNase E cleaves RNA requires interaction with a 5'-monophosphorylated end. Moreover, further evidence is presented that direct entry is facilitated by RNase E binding simultaneously to two or possibly more unpaired regions. These simple requirements may maximise the rate of degradation and processing by permitting multiple sites in a transcript to be surveyed directly without being constrained by 5′-end tethering. The specificity of RNase E is sufficiently flexible that it can cleave at some sites using different combinations of unpaired regions. Discussion within the paper includes the evolution of 5' sensing, one of the hallmarks of the RNase E family, the role of the RNA degradosome and possible new roles for RNase E. Free Full Text
Structural basis of lariat RNA recognition by the intron debranching enzyme Dbr1
Montemayor EJ, Katolik A, Clark NE, Taylor AB, Schuermann JP, Combs DJ, Johnsson R, Holloway SP, Stevens SW, Damha MJ, Hart PJ.
Crystal structures of an intron debranchilay summery gku808ng enzyme (Dbr1) are determined alone and in complex with several synthetic RNA compounds that mimic the branchpoint in lariat RNA. Together with functional data, the structures reveal the molecular basis for branched RNA recognition and explain why the enzyme lacks activity towards 3',5'-phosphodiester linkages. Dbr1 is the only enzyme known to hydrolyze the 2',5'-phosphodiester bond in lariat RNA. Hydrolysis of this bond is required before spliced introns can be metabolized or processed into essential snoRNA and miRNA. These are the first Dbr1 structures to be reported. Free Full Text
7SL RNA represses p53 translation by competing with HuR
Abdelmohsen K, Panda AC, Kang MJ, Guo R, Kim J, Grammatikakis I, Yoon JH, Dudekula DB, Noh JH, Yang X, Martindale JL, Gorospe M.
A major goal of cancer biology is to identify useful therapeutic targets. Noncoding (nc)RNAs have emerged as key gene regulators in many pathologies, including cancer. Here the authors report that the ncRNA 7SL is more abundant in cancer tissues than in normal tissues. 7SL forms RNA hybrids with TP53 mRNA, which encodes p53 (one of the tumor suppressors most often lost in cancer), and represses p53 translation. These findings uncover 7SL as the first ncRNA that binds and represses TP53 mRNA translation, and suggest that targeting 7SL may be effective in the treatment of cancers with reduced p53 levels. Free Full Text
A dimeric state for PRC2
Davidovich C, Goodrich KJ, Gooding AR, Cech TR.
Polycomb repressive complex-2 (PRC2) is a histone methyltransferase required for epigenetic silencing during development and cancer. Here we provide the first direct evidence, using multiple independent approaches, that the four-subunit and five-subunit PRC2 complexes are dimers, including eight and ten protein subunits, respectively. We are further showing that each PRC2 dimer binds a single RNA, but a single RNA can bind multiple PRC2 dimers. Our findings lead to a new molecular mechanism that can explain how a single RNA transcript can nucleate repression by binding multiple PRC2 complexes, through PRC2 dimerization and cooperative RNA binding. Free Full Text
Targeted delivery of antisense oligonucleotides to hepatocytes using triantennary N-acetyl galactosamine improves potency 10-fold in mice
Prakash TP, Graham MJ, Yu J, Carty R, Low A, Chappell A, Schmidt K, Zhao C, Aghajan M, Murray HF, Riney S, Booten SL, Murray SF, Gaus H, Crosby J, Lima WF, Guo S, Monia BP, Swayze EE, Seth PP.
Inefficient delivery of antisense oligonucleotides to target tissues can limit the efficacy of nucleic acid drugs. The authors demonstrate that a GalNac modification can enhance the potency and duration of action of two different antisense oligonucleotides targeting important biomedical targets human apolipoprotein C-III and transthyretin. The relatively straightforward chemical modification revealed in this paper improves potency by approximately 10-fold. By reducing the amount of drug needed cost will be lowered and the potential for side effects probably reduced. While the GalNac conjugates will only improve potency in the liver, one can imagine using similar modification strategies to assist uptake by other organs. The liver is already a focus for antisense therapy, so there is likely to be a significant impact on clinical development in the near term. Free Full Text
PARP-2 and PARP-3 are selectively activated by 5′ phosphorylated DNA breaks through an allosteric regulatory mechanism shared with PARP-1
Langelier MF, Riccio AA, Pascal JM.
This paper describes details of a mechanism by which two DNA damage-dependent enzymes (termed 'Poly-ADP-ribose polymerases' or 'PARPs') respond to DNA damage, through specific activation in response to generation of free 5' phosphate groups at the sites of DNA strand breaks. The study demonstrated that two PARP family members (PARP-2 and PARP-3) may exploit these interactions to act at late stages of the repair process for such strand breaks, perhaps by facilitating subsequent ligation of the cleaved DNA ends. These results provide new insight into mechanisms by which the fidelity of a genome (which is under constant assault by a wide variety of internal and external DNA damaging agents in the cell) is maintained through the action of protective repair molecules. Free Full Text
Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters
Loughery J, Cox M, Smith LM, Meek DW.
This study focuses on a key aspect of the mechanism by which the p53 tumour suppressor protein, which is crucial in preventing cancer cell survival, is activated upon receiving an appropriate stimulus. The authors show that a specific reversible chemical modification of p53 (phosphorylation) previously thought to have a limited involvement, is essential for p53 function and is required for the ability of p53 to activate the cellular machinery that leads to the manufacture of the cellular components needed to combat cancer-associated changes in the cell. This information extends our knowledge of the basic principles by which p53 functions and is controlled. Free Full Text
HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment
Taniguchi I, Mabuchi N, Ohno M.
These studies address a long standing question about the export of HIV-1 RNAs, establish a previously unrecognized function of the viral Rev protein and in so doing suggest potential new targets for anti-retroviral drugs. Free Full Text
Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification
Neumann P, Lakomek K, Naumann PT, Erwin WM, Lauhon CT, Ficner R.
More than 100 chemically modified nucleosides have been identified in tRNAs. One of them, 4-thiouridine, is found at position 8 of all tRNAs in bacteria and archaea. It stabilizes the tRNA fold and, in addition, serves as UV light sensor inducing a cellular response to UV radiation. The structural basis for the exclusive C4-thiolation of U8 has been elucidated by the crystal structure analysis of a 4-thiouridine synthetase - RNA complex. The structure revealed that the enzyme employs a molecular ruler measuring the distance from the 3'-CCA end, which is common to all tRNAs, to the site of modification. Free Full Text
Structure of human RNA N6-methyladenine demethylase ALKBH5 provides insights into its mechanisms of nucleic acid recognition and demethylation
Aik W, Scotti JS, Choi H, Gong L, Demetriades M, Schofield CJ, McDonough MA.
It is now widely recognised that nucleic acids can be marked with chemical groups, or 'tagged', in the form of methylation. Like an accented letter of the alphabet (e.g. á), these marks change the way the genetic code is 'read', resulting in a change in gene expression. Enzymes that tag nucleic acids with methyl-mark 'accents' are called methyltransferases and can be thought of as 'writers'. Enzymes that remove methyl-mark accents are called demethylases or 'erasers'. This dynamic nucleic acid modification process provides for a complex regulatory mechanism of gene expression known as epigenetics. ALKBH5 is one of two enzymes known to 'erase' N6-methyladenine (m6A) on RNA. Recently discovered cellular and physiological roles (e.g. obesity, fertility and circadian rhythm) for RNA m6A have been exciting researchers in all aspects of biology. The crystal structure presented here reveals the mechanisms of nucleic acid recognition and demethylation of ALKBH5, laying a foundation for further mutational and inhibition studies. Free Full Text
Three-tiered role of the pioneer factor GATA2 in promoting androgen-dependent gene expression in prostate cancer
Wu D, Sunkel B, Chen Z, Liu X, Ye Z, Li Q, Grenade C, Ke J, Zhang C, Chen H, Nephew KP, Huang TH, Liu Z, Jin VX, Wang Q.
Though the importance of GATA family pioneer factors in facilitating ligand-inducible nuclear receptor activity is well appreciated, the mechanism of their action is not fully understood. We show in prostate cancer cells, in which disease-related gene expression is stimulated by male hormones acting through the androgen receptor (AR), that GATA2 takes a three-pronged approach in advance of hormone treatment to prime for AR-driven gene expression. Our findings establish a hormone-independent role for GATA2 in determining chromatin accessibility of gene regulatory elements and as a component of transcription-activating chromatin loops within AR target gene loci. Free Full Text
A dynamic alternative splicing program regulates gene expression during terminal erythropoiesis
Pimentel H, Parra M, Gee S, Ghanem D, An X, Li J, Mohandas N, Pachter L, Conboy JG.
By comparing alternative pre-mRNA splicing in five morphologically distinct populations of successively more differentiated erythroblasts, the authors characterized a robust and dynamic erythroid alternative pre-mRNA splicing program. Erythroblast alternative splicing is particularly enriched in genes controlling cell cycle, organelle organization, chromatin function, and RNA processing. Differentiation stage-specific changes in splicing are executed in concert with, and may help drive, major cellular remodeling in the last stages before enucleation. A subset of splicing switches introduce premature translation termination codons, indicating that alternative splicing-coupled nonsense-mediated-decay (AS-NMD) contributes to regulation of erythroid-expressed genes as a novel part of the overall differentiation program. Free Full Text
The Vertebrate Genome Annotation browser 10 years on
Harrow JL, Steward CA, Frankish A, Gilbert JG, Gonzalez JM, Loveland JE, Mudge J, Sheppard D, Thomas M, Trevanion S, Wilming LG.
The Vertebrate Genome Annotation (VEGA) browser is a database for viewing manually curated genes on high quality genome sequence. It has recently been updated with an improved user interface to make navigating the different datasets easier. We have increased the number of annotated genomes to include human, mouse, zebrafish, rat and pig whole genomes, plus small regions of medical interest from organisms as diverse as gorilla, wallaby and Tasmanian devil. Free Full Text
TFBSshape: a motif database for DNA shape features of transcription factor binding sites
Yang L, Zhou T, Dror I, Mathelier A, Wasserman WW, Gordân R, Rohs R.
Our current understanding of DNA binding specificities of transcription factors (TFs) mainly results from nucleotide sequence analysis. However, proteins recognize DNA as a three-dimensional object. Thus, an improved comprehension of DNA binding specificity must be based on DNA structure. The TFBSshape database augments nucleotide sequence motifs with heat maps and quantitative predictions of DNA shape features for 739 TF datasets from 23 different species. Sequence information analyzed by TFBSshape is collected from the motif databases JASPAR and UniPROBE. The structural characterization of transcription factor binding sites (TFBSs) can aid users in gaining further insights into the mechanisms underlying TF-DNA recognition. Free Full Text
Cycling of the E. coli lagging strand polymerase is triggered exclusively by the availability of a new primer at the replication fork
Yuan Q, McHenry CS.
Two models have been proposed for triggering release of the lagging strand polymerase at the E. coli replication fork, enabling cycling to the primer for the next Okazaki fragment—either collision with the 5'-end of the preceding fragment (collision model), or synthesis of a new primer (signaling model). Specific perturbation of lagging strand elongation on minicircles confirmed the signaling model and ruled out the collision model. It was demonstrated that the presence of a primer, not primase per se, provides the cycling signal and that the rate of lagging strand elongation is faster than the leading strand. Free Full Text
The double PHD finger domain of MOZ/MYST3 induces α-helical structure of the histone H3 tail to facilitate acetylation and methylation sampling and modification
Dreveny I, Deeves SE, Fulton J, Yue B, Messmer M, Bhattacharya A, Collins HM, Heery DM.
New crystal structures reveal for the first time that the Double PHD Finger domain of the chromatin regulator MOZ/MYST3 can induce an alpha helical fold of the N-terminal tail of Histone H3. This enables MOZ to sample for the presence of existing histone modifications, and promotes acetylation of H3K14 by the MYST domain. A conserved double glycine hinge motif in the H3 tail is essential to allow the acetylated H3K14 residue to dock with the DPF domain. The study provides important new insights into how a chromatin regulator manipulates and modifies histone tail structure to dictate its own residency on chromatin. Free Full Text
Co-regulated gene expression by estrogen receptor-α and liver receptor homolog-1 is a feature of the estrogen response in breast cancer cells
Lai CF, Flach KD, Alexi X, Fox SP, Ottaviani S, Thiruchelvam PT, Kyle FJ, Thomas RS, Launchbury R, Hua H, Callaghan HB, Carroll JS, Charles Coombes R, Zwart W, Buluwela L, Ali S.
Most breast cancers are estrogen responsive and require the Estrogen receptor-α (ERα) transcription factor. By mapping DNA binding in the genome of breast cancer cells, we show that a distantly related transcription factor, LRH-1, co-regulates target genes with ERα, through the use of overlapping DNA binding motifs. Remarkably, LRH-1 promotes changes in chromatin to facilitate ERα recruitment, thereby leading to a co-operative regulation of gene expression. These findings provide evidence for a novel mechanism of gene regulation, whereby the binding of one transcription factor promotes the subsequent recruitment of another transcription factor to the same binding site. Free Full Text
Pre-activation of the genome integrity checkpoint increases DNA damage tolerance
Tsaponina O, Chabes A.
The genome integrity checkpoint monitors and reacts to defects in the genome, and it is well known that mutations in genes involved in this pathway cause sensitivity to DNA-damaging agents. Here, using budding yeast, we show that a low level of constitutive genome integrity checkpoint activation by hydroxyurea, an inhibitor of DNA replication, can increase DNA damage tolerance to multiple drugs, some of which are commonly used for cancer treatment. Modulation of the checkpoint activity may provide new strategies for utilizing this checkpoint to protect normal cells from genotoxic stress. Free Full Text
Promoter RNA Links Regulation of Inflammatory Pathways Genes
Matsui M, Chu Y, Zhang H, Gagnon KT, Shaikh S, Kuchimanchi S, Manoharan M, Corey DR, Janowski BA.
This manuscript lays out a new mechanism for using RNA to sequence-specifically recruit proteins and modulate transcription in mammalian cells. Free Full Text
Increasing The Complexity Of Chromatin: Functionally Distinct Roles For Replication-Dependent Histone H2A Isoforms In Cell Proliferation And Carcinogenesis
Singh R, Mortazavi A, Telu KH, Nagarajan P, Lucas DM, Thomas-Ahner JM, Clinton SK, Byrd JC, Freitas MA, Parthun MR.
Complexity is a critical characteristic of chromatin structure that allows for the myriad regulatory mechanisms that control cellular phenomena involving genomic DNA. The complexity of chromatin is currently thought to derive from the abundance of histone post-translational modifications and the presence of DNA replication-independent histone variants, such as histone H3.3, and histone H2AZ. Our manuscript identifies a source of complexity that has gone unexplored; replication-dependent histone isoforms. Free Full Text
Helq acts in parallel to Fancc to suppress replication-associated genome instability
Luebben SW, Kawabata T, Akre MK, Lee WL, Johnson CS, O'Sullivan MG, Shima N.
HELQ is a DNA repair enzyme that has been implicated in the Fanconi anemia (FA) pathway, a major mechanism to repair a specific type of lesion called DNA interstrand crosslinks (ICLs). Using the first reported Helq mutant mouse model, the authors show that HELQ plays only a minor role in ICL repair. Rather, HELQ was found to function in parallel to the FA core complex member FANCC for the successful completion of DNA replication and the promotion of genome integrity. This newly identified role of HELQ provides new insights into the etiology of FA, a human genetic disorder. Free Full Text
Attachment site recognition and regulation of directionality by the serine integrases
Rutherford K, Yuan P, Perry K, Sharp R, Van Duyne GD.
A family of enzymes known as the serine integrases are able to rearrange DNA molecules in the test tube, in cultured cells, and in live animals. They are particularly effective at delivering a new DNA sequence into a specific site in a chromosome, a process that has important implications for gene therapy and a variety of genetic engineering applications. This manuscript explain how serine integrases carry out these complex reactions, provides a plausible mechanism for their unidirectionality, and provide a much needed experimental framework for improving the properties and altering specificities in these systems. Free Full Text
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Case Study | March 30, 2011
Medilys Success StorySource: BlueStar, Inc.
MEDILYS Laboratory Improves Data Quality by Tracking Patient Samples with Datalogic's Gryphon™ and Heron™ Bar Code Readers
MEDILYS Medical Laboratories provide Asklepios Clinics and other hospitals in the area with a broad spectrum of medical laboratory services. Approximately 7.5 million tests in the field of clinical chemistry and approximately 220,000 bacteriological submissions are processed each year by five MEDILYS laboratories.
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Contact Details
BlueStar, Inc.
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Dean Reverman

subset: commoncrawl


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Dear [State Legislator],
As a resident of your district, I urge you to take action to restore meaningful constitutional limits on the federal government.
Article V of the U.S. Constitution gives state legislatures the power to act as a final check on the abuse of power in Washington, D.C. Under Article V, state legislatures have authority to hold a limited convention that can propose much-needed amendments to our Constitution. Once 34 states pass a resolution applying for such a convention, Congress must call one.
I support the approach adopted by the Convention of States Project and I want to see our state become one of the necessary 34 states to pass their model application.
A copy of the Convention of States model resolution is available by clicking here.
A copy of the Convention of States handbook, which briefly describes the Article V process and the Convention of States Project strategy, is available here.
Please support the Convention of States Project and consider sponsoring the resolution in our state.
Thank you for your time and your service to the people of our district.
[Your Name]


text:
A Place in
effective January 1,
Less than twenty
years after slavery,
several Negro
families succeed in
acquiring several
hundred acres of
rich farm land
around what is now
Tollette. In the
old days, the State
of Arkansas was
divided into
Townships. Tollette
was a part of
Township. Before
1870, it was a part
of Saline Township,
and located in
Hempstead County
Tollette is unique
because it comprises
only African
American citizens.
There is a unique
way of spelling
Tollette. The
following is the
different ways it
can be spelled:
Tolett, Toilet, and
By 1885, the
community was well
established with a
population of over
600 industrious
inhabitants. With a
view of making the
community permanent,
a petition urging
the establishment of
a Post Office was
drawn by the
illustrious Mr.
Sanford Jackson
Washington Tollette.
This petition was
forwarded to
Washington, D.C.
In 1887, the Post
Office was granted
and the community
named Tollette in
honor of Mr. Sanford
Jackson Washington
Tollette. This was
Memorial to the
splendid service
rendered to the
community by Mr.
Tollette. He was the
first Postmaster and
the father of the
town. Mr.
Tollette's wife,
Mrs. Caldonia
Crofton Tollette
also served as
(appointed February
21, 1900) after Mr.
Tollette's death.
One of the first
settlers of this
community was Steve
Tollette. Mr. Steve
Tollette owned a
large iron salt
kettle and made salt
for the surrounding
area. The kettle
weighed about 250
pounds. Some of
these kettles are
still in the area.
They are now used
for watering
stock. They came
from a salt factory
on the Saline River.
Mr. Sanford Jackson
Washington Tollette
had two brothers,
Steve Sanford
mentioned above and
John. He also had
six sisters.
Other families whose
pioneering efforts
helped to shape the
destiny of this town
include: the Croftons, Burts,
Turners, Browns,
Goodloes, Deloneys,
Alexanders, Thomases,
Hills, Greens,
Johnsons, Whites,
Jeans, Smiths,
Hendricks, Wessons,
Drapers, McGhees,
Belchers, Graves,
and many, many more.
Since 1887, Tollette
has witnessed
innumerable changes.
Each change has
served not only to
enrich the lives of
its fortunate
inhabitants, but
also to add status
to the town's
position of
Two churches nourish
the spiritual growth
of the community.
The Methodist
Church: Floyd Toland
Jeans Memorial C.M.E.
formally Mount Zion
Colored Methodist
Church. The church
site was purchased
February 20, 1884.
The church name was
changed to Mt. Zion
C.M.E. In 1954,
again, after the
General Conference,
the word "Colored"
was changed to
"Christian." In
1978, the current
building was
constructed and
renamed Floyd Toland
Jeans Memorial
Christian Methodist
Episcopal Church in
honor of the
illustrious Mr.
Floyd Toland Jeans
who served the
Connectional Church
as Secretary of
Finance from 1947
until his death in
1970. The church is
currently under the
leadership of Rev.
Orlando Sutton.
There was a Baptist
church in Tollette
many years ago. It
was listed a
Pleasant Hill
Missionary Baptist
Church. The church
was abandoned.
Later, members of
the former church
organized the Zion
Holiness Church. The
Zion Holiness church
disorganized because
of certain beliefs
and practices in the
early 1930's. It was
later abandoned. The
Church of God was
organized at the old
location. In 1938,
Rev. Betty Morrison
was called to lead
the church. Under
the leadership of
Rev. T.C. Banks, the
church added "First"
to its name. It is
now The First Church
of God. Currently,
the church is under
the leadership of
Rev. Gerald Scott.
The greatest pride
of Tollette was its
school. The Howard
County Training
School served
African American
students from
Hempstead, Howard
and Sevier counties.
Tollette was
effective January 1,
1971, and swore in
its first mayor
James A. Stewart in
The first slate of
officers included
Bobby D. Turner,
Aldermen: Chester
Cheatham, Jasper
Brown, George Young,
Tyrone Porter and
Duvall (Pete) Clark.
Under this
administration, the
first treated water
system was
More than one
hundred houses were
connected. Zebedee
White served as the
first Water
Superintendent under
the supervision of
Larry Green,
Nashville's Water
Superintendent. The
Fire Department was
established with
Nathaniel Turner
serving as Fire
A grant to remodel
homes for qualified
applicants was
approved and several
homes were
remodeled. A
softball park was
built and some
remodeling was done
to the old school
(City Hall).
1983, our second
mayor, Catherine
Thomas was elected.
Under her
plans to construct a
sewage system
were put into
action. Plans were
hampered when land
could not be
purchased (from
Dillard's) for the
completion of the
project. This
project was funded
by grants and loans
from FMHA, RDA, AIDC
and Soil and Water
Conservation. Also,
additional low
income homes were
either built or
remodeled. During
this administration
specific pieces of
playground equipment
were added to the
kiddy park.
In 1987, our third
mayor, Martha R.
Hendrix was elected.
Her council
consisted of Ricky
Gamble, Duvall
(Pete) Clark,
Chester Cheatham,
James Turner, James
Porter, and Dorothy
Walker as
Under this
administration, the
water system project
was completed and
dedicated July 8,
1993. Through loans
to low income
families, additional
houses were
constructed. Later a
new water tank and
well funded by a
combination of
grants and loans,
were installed. The
land for the new
well was graciously
donated by Mrs.
Lillie B. Draper and
son Cecil T. Draper.
In 2006, our fourth
and current mayor,
Charles Miller was
elected. His council
consists of Chester
Cheatham, Hazel
McGhee, Fred Piggee,
James Porter, James
Turner and Dorothy
Tollette has made an
indelible mark in
its history. Long
live the memory of
this unique little
town, Tollette.
Website is sponsored by The Arthur
Rupert (Banks) White Family
Webmaster Ronald A. Gordon Sr.
Great Grandson of
Arthur Rupert White
To keep this website a blessing for all
to see, we are accepting donations. We
greatly appreciate your thoughtful
donation. God Bless!


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Injection Site Rotation
The systematic switching of insulin injections from one site to another. For years, doctors have emphasized the need for people with diabetes to rotate their injection sites rather than injecting into the same place each time. Injecting into the same spot can cause lipohypertrophy, the buildup of fat under the skin, which can slow the absorption of insulin, or lipoatrophy, the wasting of fat under the skin, which can be unsightly. (Lipoatrophy is less likely to occur in people using only human insulin.)
The best places to inject insulin are the upper arms, the thighs, the buttocks, and the abdomen (at least two inches away from the navel). Because the rates of absorption vary considerably from one body region to another, the American Diabetes Association (ADA) currently recommends rotating injection sites within body regions rather than rotating to a different region with each injection.
Insulin is absorbed fastest from the abdomen, followed by the arms, the thighs, and the buttocks. (However, exercising an arm or leg after an injection can increase blood flow and speed insulin absorption from those areas.) The ADA recommends taking this variability into account when choosing injection sites. Everyone absorbs insulin somewhat differently, so the best way to find out what effect a given injection site is having is to monitor your blood glucose levels. Paying close attention to how you rotate your injection sites can help to eliminate high and low swings in blood glucose level.
Statements and opinions expressed on this Web site are those of the authors and not necessarily those of the publishers or advertisers. The information provided on this Web site should not be construed as medical instruction. Consult appropriate health-care professionals before taking action based on this information.


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China Hotels Ningbo Hotels Tongtuyuan Hotel Ningbo
Tongtuyuan Hotel Ningbo
- Location:
- Address:No. 68 Tong Tu Road, Jiangdong District, Ningbo
- Tel:87060088 (Book this hotel, call 800-2682918)
- Hotel Name in Chinese:Tongtuyuan Hotel Ningbo
- Lowest Price: $32
Tongtuyuan Hotel is adjacent to the Carrefour supermarket, Ningbo Stadium, Ningbo Exibition Center, and the bus station.
The hotel has 160 guestrooms. There is also a restaurant, a well-appointed KTV, sauna, hairdressing room, lounge, shopping center, chess and card room, and business center. Accompanying these, there are morn than 50 berths and 6 conference rooms.
Opened in 2004
- Prices not include 18% service fee and tax.
- Payment Method: pay using our secure online payment service or pay at the hotel at some hotels.
- Triple/quad occupancy may require an extra bed at extra cost.
Hotel Amenities
|Hotel Services
|Luggage service, Car rentals, Laundry service, Room service, Wake Up Call, 24hr Reception, Permanent English-speaking staff, Business Centre, Copy Service, E-mail, Translation Service, Fax Service, Typing Service
|Room Amenities
|Air conditioning, AM/FM radio, Alarm clock, Color Television, Coffee/tea maker, Telephone, Hair dryer, Iron and ironing board, Refrigerator, Bath and Shower, Free for Internet
Hotel Amenities: Elevator, Parking, Air-Conditioned Facilities, Beauty Salon, Business Center, Gift Shop, Meeting Room, Deposit Box at Front Desk, Fire Detector
Dining Facilities: Chinese Restaurant, Western Restaurant
Print the Taxi Directions
- Please drive me to Tongtuyuan Hotel Ningbo, thank you!
- 请送我到Tongtuyuan Hotel Ningbo,谢谢
- Address:No. 68 Tong Tu Road, Jiangdong District, Ningbo
- 地址:No. 68 Tong Tu Road, Jiangdong District, Ningbo
- Tel: 87060088
- Our Reviews
- Reviews from TA
Similar Hotels as Tongtuyuan Hotel Ningbo
Yun Hai Hotel Ningbo
The Ningbo Yunhai Hotel is located in the southern section of Ningbo on Chang Chun Road. It's a 17-story building as well ...
From:$ 32
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Check out the list of all 140 hotels in Ningbo
Nearby Places
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text:
Manchester Paris Emirates Airlines
Emirates Airline also called Emirates is a subsidiary of Dubai based company Emirates Group. Ranked as the biggest airline in the Middle East in terms of revenue, fleet size and passenger carried, the airline has its main base at Dubai International Airport. Emirates is the eighth largest airline in Asia in terms of passengers carried and ranked among the world's top 10 air carriers in terms of revenue. Presently, Emirates operates around 3, 715 flights per week, connecting over 100 destinations in 62 countries. Emirates is one of only six airlines in the world to operate a fleet of all wide-body aircraft. History - Emirates Airline was established by the Dubai government on 25th May, 1985. The airline began operations with flights to Karachi and Mumbai. In September, 1985, Emirates started its flight for Delhi. Initially, Emirates leased two aircraft, namely Airbus A300 and Boeing 737-700 from Pakistan International Airlines (PIA). Further, two Boeing 727 � 200 advanced aircraft w
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Transport and Travel Directions for Chiswick
UK Information » Chiswick Information Online » Chiswick Transport and Travel Directions
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SUMMARY: Email marketing strategies have matured and are no longer strictly about increasing the number of subscribers. Today, the priority lies in building a quality list of names.
We asked more than 1,100 marketers to indicate the level of effectiveness of each list growth tactic used by their organization. See the results in this weeks chart.
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Britain, and the British media, have never really used this phrase to explain events at home. When the British army was in northern Ireland, for instance, and Republican dissidents exploded a bomb, there was almost never any attempt to decipher this in the same way that would be acknowledged in foreign affairs.
The IRA or their offshoots were, for the entire media corps in Britain, terrorists pure and simple. Groups like the PLO, or the Sandinistas, were idolised by "progressive" thinkers in the 1970s and 1980s here. But making an argument that IRA bombs were in any sense part of a cycle of violence or were prompted by British occupation was virtually unthinkable. It could get you arrested, for one thing.
So to the 9/11 decade and the events that led to the July 7th bombings on the London transport system in 2005. Tony Blair had stood with George W Bush in the "War on Terror"; Afghanistan, and then Iraq, had followed. The argument over WMD in Iraq had been transformed into one that said that the world was a better and safer place without Saddam in it, to the fury of war critics. And there were multiple flashing red lights going off that Muslim sentiment in the UK was hardening, the anger growing.
Cause and effect
This is where the "cycle of violence" turns into an argument about cause and effect. Whatever led to September 11th then caused the Afghan war, and Iraq. By the middle part of the decade, when Iraq was at its worst, the security threat in the UK was at a constant level of "attack imminent". Would 7/7 have happened if Britain had not got itself enmeshed in Iraq? Was 7/7 part of the same cycle?
The view of the architects of Britain's security and foreign policies of the time would be much like their opinion of the situation in northern Ireland - that there is no moral equivalence between organised military action by the state, and the actions of paramilitaries or armed groups on the other. And past that they would also argue - as they did after 7/7 - that the principle of cause and effect cannot be used to explain incidents like the London bombings as being a reprisal. Not that that is surprising - it would imply responsibility.
All the same, it is striking how many ordinary British people do not read 7/7 in that way at all, particularly when compared to Ireland. While the anti-war movement, which saw a million people take to the streets before the Iraq invasion, has now disappeared completely, it is accepted by a large number that 7/7 would not have happened had it not been for Iraq. A poll for the Guardian newspaper, 12 days after July 7th, found that two-thirds of respondents shared this view. That does not imply sympathy, far less an acceptance that some British Muslims obviously view their religion as transcending nationality or citizenship - a point seemingly lost on the government here in the days and months before and after July 7.
For now, the security services in London say that their biggest threat remains al-Qaeda, followed by the Real IRA. While that Republican group is a rump, it will always have a constituency while Ireland is divided.
So is there a difference with the "Muslim threat"? Arguably the Arab Awakening has changed many things and has sowed as much confusion in the hearts of militant British Muslims as it did in the leadership of al-Qaeda, which was at one point advocating that Libyans should simultaneously fight against NATO and Gaddafi.
Aside from that, the coalition government in Britain - whose Liberal Democrat component was against the Iraq war and which has promised to learn the lessons from it - has the advantage of the benefit of hindsight, a beefed up internal security apparatus, and the knowledge that another event like Iraq would be impossible to sell to popular opinion.
As long as that lasts, so the chance of another 7/7 spawned by homegrown bombers looks less and less likely - ending the cycle of violence.
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Making the Most of Your Mistakes - A tale of defeat, but mostly triumphPosted by Simone Nabers in B2B Marketing on Apr 15, 2013 1:26:08 PM
So, yesterday morning I had an "oh crap!" moment (that's the PG-13 version). You know, when you send out an email blast to 11,000 people and realize that you forgot to review the subject line that still said "NEEDS TITLE"? Yeah, one of those. Most marketers have experienced this kind of flub at some point. In fact, I had a few replies assuring me that they had 'been there, done that' and were hoping to give me a heads-up so I could potentially catch the rest that hadn't gone through yet. Y'all are so nice, seriously! There are a few things that I've learned from my mistake, so I'm hoping to make the most of it and pass it along to you:
- First and foremost, I think it's phenomenal that for the majority, marketers have each others back. It would have been easy to send back a snarky reply (I'm sure I got a few of those too), but honestly, most of the immediate responses were, "Hey, just wanted to give you a heads-up..." and ".. in case you're able to fix before another send," and "I don't think you intended for this...don't worry, we've all been there". After that initial sinking feeling and 'gulp', this made me smile. So, thank you fellow marketers for the prompt words of encouragement and advice on fixing.
- On to the fix - you gotta know when to fold 'em. People know when you goof - especially smart people. You can't pull one over on most of your audience (and I hope that's never your goal). But I've found that it is far better to fess up to the mess up than try to cover up your tracks. So what did I do? I sent out a new email to the same audience letting them know that 'Oops, we messed up..." (literally - that was the new subject line) and asked them for their best advice on creating attention-grabbing invites (not like mine). And guess what? I got even more replies of understanding and appreciation for taking ownership. Here are a couple snippets from the before and after emails:
I simply added a line that most of my audience could probably relate to. Yep, I made a mistake. So here's the fix and also ask for their expertise. People love it when you ask for their help - it's authentic. Isn't that what marketing should be about anyway?
3. What's the damage? Believe it or not, the damage was far less than expected (phew!) Like I mentioned before, I received some great responses to the second email - one gentleman even told me that my first subject line did actually grab his attention and he thought we handled the situation well (although I wouldn't recommend this approach to anyone on a regular basis). Another woman replied, "Nice angle!" and another, "I like the way you fixed your mistake..." and she also gave me her suggestion for coming up with a catchy subject line like I had asked for.
If you make a mistake, you might as well learn something from it in the process.Two birds with one stone.
Also, because I'm a marketer and enjoy digging into the metrics, I pulled reports on each email and here's the break down:
Initial Email -
- 7480 sends
- 1485 opens
- 7.04% unique open rate
"Oops, we messed up..." Email -
- 7430 sends
- 756 opens
- 5.38% unique open rate
Do you see what I see? We got almost twice as many opens on the initial email, a nearly 2% increase on the "NEED TITLE" email - go figure Maybe that subject line was enough to grab people's attention to open it. Also registrations for that particular webinar, doubled!
So what can we all learn from my mistake?
- We've all made a similar mistake at one point or another. Lending a quick word of advice or encouragement creates some common ground and that's the beginning of creating a community - both with your peers and prospects.
- Fess up to the mess up, and then see if you can't get something useful out of it. People appreciate the human factor. Asking people for advice shows authenticity and that goes far.
- ALWAYS have someone double-check your email before hitting the send button.
Ok, time for confessions - what's your biggest email fail? It's for the common good, go on....


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|We had nothing in particular planned for this afternoon so drove down to Niagara to check out The Potting Shed. A few years ago they used to have a booth at the farmer's market at a nearby mall. We always intended to check out their nursery but didn't get around to it before today. They specialize in daylilies and hostas. Since daylilies are in bloom now, we thought today might be a good day to visit - and it was! (aside from the fact that it started raining....)
But there was a LOT of day lilies:
The one that really caught my eye was one called Glowing in the Dark. They dug it directly from the ground - and brought me a flower of it to be sure they were digging the right one:
There were a lot of hostas too - but far fewer than daylilies. See the dark purple flowers in this picture? I bought one of those. I need to check the tag to be sure - I think it's called Rosedale Bernie - or maybe Bernine. I did an internet search and can't find it so I think it's a new introduction. The flower color was very striking.
I also picked up the more commonly found Blue Mouse Ears (which I haven't seen locally) and a Blue Cadet. I was awfully tempted by Country Mouse - it is SO cute!
There were a lot of lilies too - here are a few that caught Randy's eye:
There were other plants too. I bought this bleeding heart called 'Burning Heart' - this is not a good picture; the flowers are a very intense red:
I was tempted by this coneflower 'Coconut Lime':
They had a small collection of water plants. We liked this waterlily:
And someone was obviously playing around with grafting!
It was a fun visit and we'll definitely go back again sometime. I did wonder if Saucy has been there...
Here is a link that might be useful: The Potting Shed
Follow-Up Postings:
- Posted by triple_creek z5 (My Page) on Tue, Jul 21, 09 at 22:22
|Glowing in the Dark will fit right in at your house Woody.
I like Country Mouse too.
The third Lily is my favorite.
Fun little outing.
We went to Niagra once while visiting our daughter when she lived in PA.
|You don't seem to run out of destinations you two!!! Seems they had plenty to offer, even a clematis! ;)
The dark red lily looks really nice...even against your rain jacket!
Where is your little mouse going to be planted?
- Posted by prairiemoon2 zone 6/MA (My Page) on Tue, Jul 21, 09 at 23:04
|Woody, what a fun trip, I enjoyed that! I like the one of you lost in a sea of dayliies. [g] And I just started Laughing, when I realized that I've been humming 'Dancing in the Dark' since seeing your daylily. [g] That's a very pretty, saturated color! I had not seen 'Country Mouse' before, I like it. I think the tiny hostas are so cute, but I haven't seen a situation around the garden where I could appreciate their size, yet. Our Casa Blanca Lilies are about to open soon. I've had enough success against the darn Red Lily Leaf Beetle this year, to consider getting a new one. I LOVE lilies. Third and Fourth and Last are my favs. Pretty color waterlily too. .....I also loved the photos you posted of your evening with Marie and company. Looks like you had a fun time. I have never tried quinoa and it is supposed to be good for you, right? What does it taste like? ..... Thanks for the list of plants in your bed. :-) I was going to ask what the white was in your beds, but I think it might have been the White Clips. I am enjoying my Clethra 'Hummingbird' which is just a little bit taller than the 'Sixteen Candles' Even out of bloom, the foliage is attractive and it has been very easy care so far. You have 'Pink Beauty' potentilla in other beds, don't you? Nice for flow. I have 'Silver Mound' and it's not doing too well this year. Too much rain and not enough sun, maybe. Glad you two are enjoying yourselves!
- Posted by michelle_zone4 zone 4 NW IA (My Page) on Tue, Jul 21, 09 at 23:05
|It looks like a fun place to visit but one that has lots of temptations LOL I have 'Blue Mouse Ears' and 'Blue Cadet' in my mini hosta bed. It seems that that retirement is being enjoyed by both of you.
|Yes, we're finding lots of interesting destinations for day trips. When the City or Toronto workers currently on strike go back to work, we'll probably visit the gardens at Spina House - similar era to Casa Loma but more garden from the sounds of it.
gb - I didn't see any other clematis, just the one in the entrance. A pity....:-) The hostas will go under the newer white redbud - at the SE corner of the back lawn behind the log edge, against the path to the south alley. It's about the only spot I can think of where they won't be overwhelmed by other plants. That corner is largely forget-me-nots that get ripped out after blooming so the area is fairly bare. I'm going to put the red bleeding heart in the midst of some white corydalis. I think that will make it really stand out!
PM - Marie was the photographer for the pictures of Phoebe and dinner but was using our camera which is why I had the pictures to post :-) Quinoa is interesting - the only significant edible grain from the New World. (I think it's technically not a grain because it doesn't come from a grass...) It is also high in protein and the only grain that its a complete protein on its own. In its natural state the outer coating is poisonous because it contains oxalic acid so the natural stuff needs thorough washing and soaking to get rid of it. Most quinoa sold in North America has been washed and cleaned already. (I had to look all that info up when we cooked it the first time because I didn't know much about it...) The taste is light - maybe because I've, so far, only cooked it with lemom in it so that makes it light-tasting. It's a rice substitute in meals so try it with any meal where you'd use rice. There's a red variety too that we haven't tried yet. Yes, the white is 'White Clips' and yes, I have lots of Pink Beauty in the driveway border - it's one of my favorite shrubs.
Michelle - it was a dangerous place for the wallet!
|Looks like a fun trip. I see a pink Day Lily with a red center in the foreground of the third pic that I like. I like seeing you in the pics, too. :-)
That is a pretty bunch of Lilies that Randy picked.
I have not tried any of the miniature hostas. Like Pm2, I don't have a place in my yard to put them.
|Wow, what a fun place!!!! I love that 'Glowing in the Dark!What a great color! I really love my 'Blue Mouse Ears'and would love to eventually have a large patch of it. Thanks for sharing your trip.
- Posted by dodgerdudette NapaCaz9 (My Page) on Thu, Jul 23, 09 at 7:12
|I saw 'Blue Mouse Ears' for the first time 'in person' at Wendys yesterday---so cute !The daylily you selected is a pretty one Woody-
Thanks for the tour !
- Posted by drema_dianne z6OH (My Page) on Wed, Jul 29, 09 at 7:45
|Woody, looks like you had a fun day. I love all kinds of lilies, and it is hard to resist taking them home. I really like the Coconut Lime Coneflower. I can't grow anything that has Daisy like petals. Not even the original pink coneflower. But I keep trying:) One day I will find the one that likes my soil. My mom has a lot of clay, and she can grow all of them. I keep trying to make may sandy dirt more like hers, but I think it all washes away underground somewhere:)
thanks for the pics.
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From a vintage chest turned into a kitchen island to upside-down planters used as tables, we've got unique ideas to make your old furniture seem like new again.
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Mon, 28 November 2011
David Pescovitz and Mark Frauenfelder speak with Jon Ronson, a journalist, documentary filmmaker, and the author of the bestselling books, The Psychopath Test: A Journey Through the Madness Industry and The Men Who Stare at Goats.
Jon's latest work is a very short book, or long article, available as an ebook, called The Amazing Adventures of Phoenix Jones, which explores the growing phenomenon of real life superheroes. It's available as an eBook through Amazon and other online ebooksellers. (Photo of Jon Ronson by Barney Poole)
Here's Jon's descriptions of his interests:
Direct download: gweek_028.mp3
Category: -- posted at: 4:14 AM
Fri, 18 November 2011
In this episode of Gweek, Mark Frauenfelder is joined by Boing Boing's managing editor Rob Beschizza and Seth Godin, an author, entrepreneur, and human wunderkammer.
Here's what we talked about:
The Domino Project: Seth's partnership with Amazon to publish short books loaded with useful ideas
Searching for an adjustable standing desk that doesn't cost a fortune
Test driving the Mitsubishi i electric car
The undiscovered or overlooked or underappreciated work of John Taylor Gatto, eduction reformer
A wonderful how to book called Handmade Music Factory: The Ultimate Guide to Making Foot-Stompin'-Good Instruments
Penguin Magic Shop: An online magic trick store with good video demos
Sasha Dichter: Reflections on generosity, philanthropy and social change
Collin's Lab: excellent introductory videos to electronics
Seth's fondness for buckwheat soba noodles
Bobo Explores Light: A swell iPad app that teaches kids about light
Rising Card: A great $0.99 iPhone magic trick
Kaweco Liliput Ballpoint Pen from JetPens
Sous Vide Turkey for Thanksgiving
Download Gweek 027 as an MP3 | Subscribe to Gweek via iTunes | Subscribe via RSS | Download single episodes of Gweek as MP3s
Direct download: gweek_027.mp3
Category: -- posted at: 10:15 PM
Mon, 14 November 2011
NOTE: If you did not get Gweek episodes 24 - 26, then please re-subscribe using this feed URL.
In this episode of Gweek, I'm joined by Boing Boing's managing editor, Rob Beschizza, and Joel Johnson, editorial director of Kotaku.
Here's what we talked about: The Elder Scrolls 5: Skyrim Rob has been playing it incessantly but says it feels "like walking around a ren faire where everyone is heavily medicated." Skylanders: Spyro's Adventure Starter Pack Minecraft is going 1.0 Kotaku's upcoming gift guides Make's Ultimate Kit Guide Joel read The Postmortal tng_s8 Mixel - a free iPad collage app Mark's Dungeon Raid addiction relapse Mophie Juice Pack Plus Outdoor Edition iPhone 4S and Siri
Download Gweek 026 as an MP3 | Subscribe to Gweek via iTunes | Subscribe via RSS | Download single episodes of Gweek as MP3s
Direct download: Gweek_026.mp3
Category:Video Games -- posted at: 7:54 PM
Sat, 5 November 2011
In this episode of Gweek, Ruben Bolling and I are joined by the cartoonist and illustrator Michael Kupperman, who has a hilarious new book out, called Mark Twain's Autobiography 1910-2010. Ruben interviewed Michael about his work and then the three of us went on to talk about the folllowing things:
I got rid of my standing desk last week and am looking for a better solution.
Using an hour-timer as a self-check throughout the day
Esther Williams' autobiography, The Million Dollar Mermaid
Steve Jobs, by Walter Isaacson
Cul de Sac Golden Treasury, by Richard Thompson
Uni-watch: The Obessive Study of Athletic Aesthetics
The Walking Dead TV show
Download Gweek 025 as an MP3 | Subscribe to Gweek via iTunes | Subscribe via RSS | Download single episodes of Gweek as MP3s
Direct download: Gweek_025.mp3
Category:Video Games -- posted at: 2:41 AM


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News 1 - 5 of 5
Airline Official Denies Brandon T. Jackson's N-Word AccusationNovember 06, 2013 02:11:54
Jackson, who previously was escorted off of a U.S. Airways flight, writes on Twitter that a flight attendant launches racial slur against him.
CBS' 'Beverly Hills Cop' Pilot Finds Its Lead in 'Tropic Thunder' StarNovember 14, 2012 02:11:39
Brandon T. Jackson, who also stars in 'Lottery Ticket', is cast as a blue-collar police officer who is the son of Eddie Murphy's Axel Foley.
Brandon T. Jackson's 'Imma Do It Big' Video From 'Big Mommas'February 10, 2011 04:02:21
Featuring T-Pain and One Chance, the music video mixes Brandon T. Jackson's performance scenes and footage from movie 'Big Mommas: Like Father, Like Son'.
Martin Lawrence Coming to 'Big Momma's House 3'March 02, 2010 04:03:54
Lawrence is set to reprise his role as Malcolm Turner, joined by 'Percy Jackson and the Olympians: The Lightning Thief' actor Brandon T. Jackson who plays his nephew.
Rosario Dawson Refuses to Be Brandon T. Jackson's MentorFebruary 03, 2010 03:02:20
Rosario Dawson, who shares screen with Brandon T. Jackson in 'Percy Jackson & the Olympians: The Lightning Thief', declines her co-star's request to be his mentor because she doesn't want to feel old.


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Workshop or seminar - what's the difference?
The main difference between a workshop and a seminar simply comes down to numbers. The DPL workshop series are restricted to no more than 24 participants, and that capacity is usually reached quite quickly. The small group format is designed to facilitate the interactive nature of the workshops.
Seminars, however, are only restricted by the seating capacity of the venues we use and are free to members and their non-registered staff. Interaction is more formal and questions are usually moderated by a chairman.
Non-member practitioners are welcome to attend both workshops and seminars for a fee.
DPL Australia Pty Ltd ('DPLA') ABN 24 092 695 933, CAR No. 326134 is a Corporate Authorised Representative of MDA National Insurance Pty Ltd ABN 56 058 271 417, AFS Licence No. 238073. DPLA members have access to the Dental Indemnity Policy underwritten by MDA National Insurance Pty Ltd.
DPLA is a wholly owned subsidiary of the Medical Protection Society Limited (MPS) (registered in England No. 36142) at 33 Cavendish Square, London, W1G 0PS. 'DPL member' and 'DPLA member' means a non-indemnity dental member of MPS served by DPLA. The benefits of MPS membership are discretionary and set out in its Memorandum and Articles of Association. Neither DPLA nor MPS are insurance companies.


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Anglesey Abbey, Gardens and Lode Mill
Gift Aid
Whole property
- Adult: £11.90
- Child: £6.30
- Family (1 adult): £19.30
- Family: £31.20
- Group adult: n/a
- Group child: n/a
Gardens only
- Adult: £7.30
- Child: £3.90
- Family (1 adult): £12.30
- Family: £19.60
- Group adult: n/a
- Group child: n/a
Whole property
- Adult: £10.80
- Child: £5.60
- Family (1 adult): £17.30
- Family: £28.10
- Group adult: £9.70
- Group child: £4.80
Gardens only
- Adult: £6.60
- Child: £3.50
- Family (1 adult): £10.90
- Family: £17.50
- Group adult: £5.80
- Group child: £2.90
Discounted rate when arriving by cycle, on foot or public transport
Find out about Gift Aid admission
Most of our places run the Gift Aid on Entry scheme at their admission points. Under this scheme, if you're not a member you have the choice of two entry tickets:
- Gift Aid Admission; or
- Standard admission
If the place runs Gift Aid on Entry, we'll offer you the Gift Aid Admission prices. But it's entirely up to you which ticket you choose. If you want the Standard Admission instead, just let us know when you come to pay.
Gift Aid Admission includes a 10 per cent or more voluntary donation. Gift Aid Admissions let us reclaim tax on the whole amount paid* — an extra 25 per cent — potentially a very significant boost to our places' funds.
An extra £1 paid under the scheme can be worth over £3 to the National Trust as shown below: Payment of the additional percentage donation is entirely voluntary, so if you prefer to pay the standard admission please advise our reception staff at the till point.
|Amount paid
by visitor
|Tax refund from Government*
|Total received by the National Trust
|Gift Aid admission
|Standard admission
Money raised from this scheme goes towards vital conservation and restoration projects at the place you're visiting.
Most National Trust members already pay their subscriptions using Gift Aid, helping us to reclaim many millions of pounds every year at no extra cost to themselves. If you are a member and are not already paying in this way, you can make a Gift Aid declaration to the Trust online. Or you can make your declaration by simply telephoning our Supporter Services Centre on 0844 800 1895.
*Gift Aid donations must be supported by a valid Gift Aid declaration and a Gift Aid declaration can only cover donations made by an individual for him/herself or for him/herself and members of his/her family.


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Big Ears Festival Initial Lineup: Bryce Dessner, Terry Riley, Kronos Quartet ...
Excited as we were to hear about Big Ears Festival Artists-in-Residence, and Classicalite favorite, Kronos Quartet making their way to Knoxville in 2015, we are overjoyed with the initial line up announcement. From March 27 to 29 contemporary classical ...
Big Ears Festival announces 2015 lineup, featuring Max Richter, Grouper, Laurie ...
Tiny Mix Tapes (press release)
Now it's time to provide some meat to Kronos' bun overlordship, as Big Ears has just revealed the 2015 edition's fantastic initial lineup. It includes: Swans, Grouper, Ben Frost, A Winged Victory for the Sullen, tUnE-yArDs, Amen Dunes, Holly Herndon, Clark, ...
Laurie Anderson, tUnE-yArDs, Terry Riley, Swans and Kronos Quartet Top Big ...
Nashville Scene
In its short history, Knoxville's boutique Big Ears Festival has brought together some incredible lineups, overflowing with innovative artists you're not going to see on the mainstream festival circuit — composers and performers whose specialize in breaking ...
and more »
A Season's Worth of Music in a Weekend at Knoxville's Big Ears Festival
New York Times (blog)
Big Ears, a festival in Knoxville, Tenn., in which indie classical, pop and jazz composers and ensembles rub shoulders, has announced its 2015 roster, and it reads like a season listing for Greenwich Village and Brooklyn, compressed into a single weekend, ...
and more »
Big Ears Festival Announces 2015 Lineup Featuring Laurie Anderson, TUnE ...
Discover who made the lineup for Big Ears Festival's 2015 return. Prepare yourselves for a weekend of creative expression and music appreciation, Knoxville, Tennessee! big ears festival. Knoxville, Tennessee's Big Ears Festival has released its official 2015 ...
and more »
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My interview at a Hospital with the lions
- 0Sep 12, '13 by garn12I had an interview at a Hospital this morning in Ga for one of their ICU units. Basically the interview went horrible and I was ripped to shreds from the moment they walked in through the door. I was interviewed by two nurses and the director. I went to shake their hand one of the RNs did not even extend to shake my hand upon greeting. The entire interview process made me feel horrible.
My background: I graduated from nursing school with my BSN July 2012. I was an extern (float nurse's aide) from December 2010-last October 2012. I have one year experience as an RN on a med surg ortho/spine surgical unit in a level two trauma hospital. I am chair of shared governance on my floor, and I have also charged quite a few times on my unit.
They told me verbatim that "I have no experience". They implied to me that if I get a job here I need to know how to access a patient which offended me because I felt like they were basically telling me I don't know how to assess a patient which is something all nurses do. They asked me if I had any experience with central lines and foleys. They laughed in my face when I told them my hospital I currently work at has an IV team.
At times I would answer questions they cut me off and even re asked the same question which led me to believe that they were not interested to begin with. I would have personally preferred to receive an automated rejection response from HR than to sit in an interview for 30 minutes and be hazed and asked do I know what I'm getting myself into by coming to work here.
The hospital looked nice on the inside and I really feel bad because I want to learn and grow as a nurse and this is the best hospital to be at. However, I felt degraded and as though I haven't accomplished anything at all. The unit director asked me what was our patient satisfaction scores on the unit I work for. I actually don't know that answer but I know it is accessible to the public if she was curious. I thought the interview was about me not the unit I work for???
The unit director then left in the middle of the interview and let the other two nurses finish. Where is nursing going these days? Why are people so rude? If I met the qualifications for applying for the job and I was offered the interview why was it necessary to bring me in to humiliate me?Last edit by Joe V on Sep 18, '13
- 32Sep 12, '13 by Esme12, BSN, RN Senior ModeratorHoly Smoke!!! THANK GOD!!!!! They showed their colors in the interview!!!!
Could you imagine getting the position and having to work with these people? I would rather stab myself in the eye!!!!
That was a close call...they could have been your co-workers.... your guardian angel was looking out for you!!!
Celebrate your near miss and move forward....((HUGS))
Welcome to AN! The largest online nursing community!
- 10Sep 12, '13 by Dranger, BSN, RNScrew that! If I was being put into a situation like that I would have gotten up and left. IF the interview is like that, think what working there is like?!
- 4Sep 12, '13 by FineAgain, BSN, RNThey treated me like that when I went to Grady Nursing School (back when there was such a thing). Lowered my confidence so much that I didn't go back to school for years. I'm a happy BSN now though. Be glad of your good luck in not being hired. You sound like an awesome nurse with great qualifications.
PS You should have replied "why no...what is a foley anyway? I've heard of them and always wondered".
- 1Sep 13, '13 by woohIf the interview made you feel "horrible" you'd never make it working there. Especially in the ICUs. Staff there is NOT warm and fuzzy. They know their stuff and if you're in a trauma somewhere in the state, you're going to be lucky if you end up there. But warm and fuzzy is not their culture.
- 3Sep 13, '13 by AJPVMaybe Johnson and Johnson will want to come to that hospital to shoot some video for their Discover Nursing campaign. What a great recruitment tool for our profession! Ha ha ha! Wow, seriously, that's pretty unbelievable. You should send them a pamphlet for a remedial reading course since they apparently struggled with reading comprehension when they had your resume in hand before THEY DECIDED to call you in for an interview. Wow.
- 3Sep 13, '13 by not.done.yet GuideThat is appalling. One can be an exceptional nurse/clinician and still have good manners. If their culture really is a stoic and harsh one, this may have been a tactic to weed out those who aren't able to survive or thrive in that environment. I think that would rule out the majority of nurses who are still young in their practice, which may be no accident. I am sorry you had to go through that. Remember, their behavior is a reflection of them, not you.
- 1Sep 13, '13 by NurseCardQuote from woohWow... well, I'd never make it there, that's for darn sure. That interview would have made ME feel like crap, and I've been a nurse for ten years.If the interview made you feel "horrible" you'd never make it working there. Especially in the ICUs. Staff there is NOT warm and fuzzy. They know their stuff and if you're in a trauma somewhere in the state, you're going to be lucky if you end up there. But warm and fuzzy is not their culture.
- 6Sep 13, '13 by The_OptimistA little pressure and you balk. I notice you have a lot of "I am this or am that...". I applaud you. It is always good to recognize your efforts, but also recognize that in a different situations, those skills might mean zit. It takes humility to recognize that. Sadly, not everyone has that.
Why be so quick at taking offense? And another suggestion, be better assertive. Assertive does not mean being rude, it means standing up for yourself (even if you are seething on the inside).
Learn from the experience- take what matters and discard the rest. Success in your next step.


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Although the American Journalism Review predictably bemoans the state of state house news coverage around the country, the magazine recently looked in on some promising profit and nonprofit start-ups. Nationwide, there are very few reporters covering state politics. A little over a year ago an AJR survey found that there were only 355 reporters covering state capital issues, down one-third from 2003.
AJR's Mark Lisheron starts off in New Jersey, a state where corruption is "taken for granted by its residents" and the FBI, checking in on new outlets like NJ Spotlight, founded by veteran Garden State print reporters, and Statehousesteps.com, set up by a state house radio correspondent. Liseheron also spotlights developments in California and in Texas, where the nonprofit Texas Tribune is being closely watched.
AJR also gives a shout-out to an organization familiar to Bay Staters, which has found a successful pay formula. Boston's State House News Service, the online, subscription-only news outlet, set up a similar operation, the News Service of Florida (complete with a jazzier website than its Hub cousin), in Tallahassee. The Florida news outlet aims to attract a similar subscriber base: state officials, lobbyists, advocacy groups, and law firms willing to pony up more than $1,000 a year for a "comprehensive Statehouse wire service e-mailed to the customer." News organizations also pay for its coverage.
The Florida news service was launched by Craig Sandler, Russell Pergament (the first publisher of Boston Metro) Stephen Cummings in 2008. Sandler is the owner of the Boston statehouse news organization. Cummings is a friend of Pergament's with whom he co-founded the Brookline-Newton Tab. They also helped Sandler fund the purchase of the Boston statehouse outlet.
The News Service of Florida is becoming "the place of record" for the Florida statehouse, according to Mary Ellen Klas, one of the heads of the recently combined Miami Herald and St. Petersburg Times state house bureaus.
National politics
Congressional Democrats are preparing to trade extension of the Bush tax cuts for renewal of unemployment benefits, according to this New York Times report.
Springfield's Richard Neal is making a play become the ranking Democrat on what will soon be the GOP-controlled House Ways and Means Committee, reports the Globe.
The New Republic's John McWhorter has some interesting food for thought on the George W. Bush's apparent anguish over the bigot charge leveled at him after Hurricane Katrina by rapper Kanye West.
The Tea Party-fueled campaign against Congressional earmarks would tilt more power to the executive branch (and the socialist president currently in office there), say two California political scientists writing in the National Journal.
State politics
Fresh off his reelection victory, Gov. Deval Patrick says he's committed to program set of initiatives to more fully integrate immigrants into state life, including charging in-state tuition rates to illegal immigrants, an issue that has roiled the political waters in recent years.
Jim Braude, on NECN, interviews Bruce Tarr of Gloucester and Robert Hedlund of Weymouth, the two Republicans seeking to lead the "gang of four" in the Massachusetts Senate.
Business and the economy
Two front-page stories in today's Globe make for an unhappy pairing. Charities in the state say they are receiving a record number of calls for help with food, heating bills, and other essentials by residents battered by the bad economy. Meanwhile, charitable giving is up this year compared with last, but still lags considerably behind pre-recession levels, according to a new report from the Boston College Center on Wealth and Philanthropy.
Steven Antonakes, the state banking commissioner, is heading to DC to oversee the marketing of consumer products by banks in the new Consumer Financial Protection Bureau established by Congress in the wake of the financial crisis.
Peter Diamond, the Nobel Prize-winning MIT economist, seems to be on his way to a seat on the Federal Reserve Board. A bid by Alabama Republican Sen. Richard Shelby to scuttle the nomination on a technicality failed, as the Senate Banking Committee voted to send Diamond's nomination to the full Senate for a vote.
Health care
State officials are serious about keeping mandatory health insurance mandatory, the Herald reports. To date, they've denied 3,150 appeals of fines stemming from not being insured.
The Washington Post reports that the Illinois company that makes Four Loko, which college students call "blackout in a can," announced Wednesday that it plans to remove caffeine and similar chemicals from all of its products. The FDA is expected to announce shortly that caffeinated alcoholic drinks are unsafe, which would be tantamount to an outright ban. Both New York and Massachusetts have been considering bans and New Jersey and Washington state have linked the drink to cases of alcohol poisoning.
With the holiday flying season fast approaching, the Patriot Ledger takes the temperature of some passengers at Logan Airport over whether they prefer full-body scans or someone coming near their "junk" in a pat down and how the heightened scrutiny affects their travel.
Massachusetts does one of the worst jobs of combating repeat drunk drivers, according to the National Transportation Safety Board.
Energy and environment
The Salem News says Salem Harbor Station should be converted into trash transfer depot.
The Globe editorial page says Boston should go ahead and adopt more stringent energy efficiency requirements in its building code.
Municipal affairs
The city of Quincy and the private Woodward School for Girls are locked in a legal battle over money from a trust set up by President John Adams, the Globe reports.
James Stone and Wendell Knox, in an Globe op-ed, say urban schools can make big strides without huge structural makeovers. The keys: strong school leadership and relentless use of data to drive decisions.
Sign of the times
The Lynn Item reports on a man caught stealing aluminum park benches.
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text: Testimonial – Eva Slover By Reid Walley on June 2, 2012 in Testimonials "Thank you, again, for your brilliant advice. You sure are talented at getting to the root of things. Much appreciated, Reid!" Eva Slover Advocate for empowering communities through mind-body health and wellness Sacramento, CA May 12, 2012


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Hello, My name is Daisy Arriaga and welcome to my Web Profile. I am a current senior at Ithaca College, majoring in Journalism and minoring in Legal Studies. I will be working on my Master's Degree in Social Work at Herbert H. Lehman College in New York City in the Fall 2013 semester.
Below you will see my current involvement, if you would like more detail about my involvement feel free to read my biography, linked on the left side bar, along with work that I have completed inside and outside of the classroom.
Feel free to check out my Photo Galleries where I have photos of my travels during my semester abroad and my involvement on campus.
Current Involvement :
She's the First*{Ithaca} | Founder and President
Admissions Student Ambassador | Peer Recruiter


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Most of the time, audiences get their first glimpse of an Opera Carolina production when the curtain goes up. But the Mint Museum Uptown helps set the scene for January's "Madama Butterfly."
"Jun Kaneko: In the Round" spotlights the artist whose sets and costumes give "Butterfly" a new look: a colorful, dramatic transformation of traditional Japanese styles.
The exhibition, running through April 28, puts some of Kaneko's ceramic sculptures - which have been his specialty for decades - alongside his drawings for "Butterfly," his operatic debut. The show will expand outdoors in January, when a 12-foot sculpture of Kaneko's (photo courtesy of Jun Kaneko Studio) replaces the giant red Christmas-tree ornament in the Mint's plaza.
Kaneko was born in Japan in 1942. He moved to the United States to study in 1963, and he has long resided here. One of his mentors, ceramic artist Peter Voulkos, has described Kaneko's creations as "an amazing synthesis of painting and sculpture ... intellectual and playful."
By working together, Opera Carolina and the Mint "increase appreciation for a renowned artist by showcasing a variety of mediums of his work," Mint president Kathleen Jameson said in a statement. People who see Kaneko's work in both the galleries and theater "can experience the continuity of form, pattern and design between these two modes of expression and creation," curator Carla Hanzal said.
Kaneko, who has been based in Omaha, Neb., for two decades, designed "Butterfly" for Omaha's opera company. Opera Carolina will return to him when it presents his new production of Mozart's "Magic Flute" - shared with the opera companies of San Francisco, Washington and Omaha - in January 2013.
For a taste of his "Butterfly," you can see a slideshow on Kaneko's website, www.junkaneko.com. Click on Studio, then Artwork, then Performances. But do yourself a favor. Look at the first five or 10 images - enough to get a taste - and stop there. That will leave you something to discover in the theater.
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Have a news tip? You can send it to a local news editor; email email@example.com to send us your tip - or - consider joining the Public Insight Network and become a source for The Charlotte Observer.Read moreRead less
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And finally, as Mark Twain said: "Always do right. This will gratify some people and astonish the rest."
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On my profile I put a vido of Mike Pulcinella on his 6 foods that work...they were oatmeal, eggwhites, chicken, brown rice/sweet potatoes, tuna, and BANANAS. How many bananas and when do you eat them??? I have always heard they go straight to your ass because of its higher GI....I know more unripe bananas are better than the riper ones (GI wise) but anyone with info....Please share!! Trying to cut the fat the quickest way possible so any other tips are welcome Thanks
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Who do you think looks best today? (5 photos)
The best street style from London Fashion Week: http://ow.ly/p48HS via @StyleCaster
Beauty Products We Love
Our holiday fragrance lineup just got more decadent thanks to #Marni's...
WHAT SHE WORE Moore added Bulgari gems to her bow-bedecked tulle Stella McCartney dress for the N.Y.C. debut of Crazy Stupid Love.Read MoreLess


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wooah hey ppl kelly here...woot woot! im happy! i just found out zach is coming home in two weeks so me and britt are happy about that woot woot zach! lol -_- lol i went to canada yesterday woot! i had FuN fUn lol i went to the scottish store <3 cuzzz im scottish <3 lol woot! i bought a pin that is plaid and says up yer kilt hehe and a bumbersticker that says if its not scottish its crrrrr~ap woot woot! lol i <3 canada hehe i wanna see that movie the village..i had a dream about that once before the movie came out...they stole my idea....kill them!! lmao wooah i have run outta things to say :p blah this whole thing is boreing anyways! i dont think people even read this so its kewl! woot woot! l8rz!
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Since 1975 Pike and Western has been helping customers find the perfect wine for any occasion.
From the beginning we have had a local focus, the wines of Washington and Oregon. But we also stock an extensive selection of European wines, many from people we have built relationships with over the years. We like to call ourselves a local wine shop with a world view.
Drop by and taste some fresh new stuff.
We taste here in the store every Friday
afternoon from 3:00 to 6:00pm. Hope to see you!


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Have a question? Need an estimate?
Want an "in" to the best sushi joint in town? Just call us at 716.855.8550 (we're usually here from 9ish until at least 6ish during the week), or fill out the form on this page and we'll get right back to you.
Thank You For Contacting Ingenious, Inc.


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Welcome to Counseling and Psychological Services (CAPS)!
CAPS is a unit of the division of Student Affairs of Sonoma State University. CAPS offers confidential counseling to students experiencing personal problems that interfere with their academic progress, career or well being.
Please note:
The CAPS web pages provide information only. If you would like to talk with someone or make an appointment, please call (707) 664-2153 between 8 a.m. - 4:30 p.m. Monday-Friday.
For immediate response when CAPS is closed, please call SSU Police Services at (707) 664-4444 or Sonoma County Crisis Line at (707) 576-8181. Both are available 24 hours a day.
**Coming Soon**
No registration is required. Walk-ins welcome.
Groups Forming Now
Please call CAPS at (707) 664-2153 for more details.
General Therapy Group:
Members of group therapy benefit from a supportive community of students dealing with similar issues. Group members are working on adjusting to college, managing emotions, improving relationships, and self-awareness. One of the best things about group therapy is getting support and feedback in a safe environment. The group meets weekly, and generally consists of four to eight students and a therapist.
Groups Running Now
(New Members Welcome)
Bisexual Discussion Group:
Join our weekly discussion of topics related to bisexuality and explore your own sexual identity. The group is open to students who identify as bisexual, pansexual, queer, fluid, or any other non-binary sexual identity label, as well as students who may be questioning if one of these labels fits for them. Group meets on Thursdays at Noon in Stevenson 3044. Drop-ins welcome. Contact Lindsey Brooks: firstname.lastname@example.com with questions.
Women of Color Collective:
Providing an opportunity for women of diverse cultural backgrounds to come explore, process, and support each other in a safe space. Let's create community and learn about ourselves!
Survivors of Sexual Assault:
This group honors each survivor's unique experience and healing process and provides a confidential and safe space to discuss concerns and receive support. There is also an opportunity for group members to become more active in sexual assault awareness and prevention as desired. Contact Laura Williams at firstname.lastname@example.com with questions.
Please Note: Communication over the internet is not secure. We do not provide personal counseling over the internet. Please do not send by email any information you consider privileged or confidential.
Take a mental health screening here!