text:
(156 reviews)
|Average Rating.
|Would buy this product again.
Glamagogo77 on 12/24/2013 7:00:00 PM more reviews by Glamagogo77
Age: 19-24 Skin: Very Dry, Fair Hair: Brunette, Straight, Fine Eyes: Brown
"Sex Bomb" is one of the go-to Lush flavors (I guess you could call it that). I believe that it is one of their best selling bath bombs and I can see why. It is sexy, feminine, and very floral. As others have mentioned the scent is very strong (which some people may or may not like). I however, happen to love it - especially after a long stressful day at work where I just want to come home and relax in a warm bath. Some people have commented that Sex Bomb stains the tub, it does a little bit - but it's an easy clean (the next day, because you know after a sex bomb bath you just gotta relax!)
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Ramalam on 10/27/2013 2:25:00 PM more reviews by Ramalam
Age: 19-24 Skin: Combination, Medium Brown Hair: Brunette Eyes: Brown
A lovely flowery, fruity smelling bomb! The smell is pretty pungent, so for people who are sensitive to strong smells, beware! I couldn't fault it, the smell lingers on my skin for most the day and makes my house smell great! It doesn't do anything for the condition of my skin but I wasn't expecting it to. And I've had no issues with it staining my tub. The little flower in the centre dissolves and runs straight down the sink. Would purchase again when I need refreshing!
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thechain on 9/29/2013 8:31:00 PM more reviews by thechain
Age: 25-29 Skin: Combination, Fair Hair: Brunette Eyes: Blue
This bath bomb is very...blah. I don't find it "sexy" at all. The color is pretty (but stains the tub!). The smell is okay, but nothing special- particularly when you compare the other delicious scents Lush has.
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brookekthnx on 9/24/2013 10:36:00 PM more reviews by brookekthnx
Age: 19-24 Skin: Combination, Medium Hair: Brown Eyes: Brown
Over all smell is amazing (very feminine and clean) but the only downside was the stains left behind on my tub, yikes! But I will be purchasing this again
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clarissa90 on 5/18/2013 11:08:00 PM more reviews by clarissa90
Age: 19-24 Skin: Acne-prone, Medium, Cool Hair: Brown, Wavy, Coarse Eyes: Brown
I'm not sure what the hype is surrounding this product. Luckily, I got this as a gift from a friend. I will not purchase.
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vw1983 on 4/22/2013 10:12:00 AM more reviews by vw1983
Age: 30-35 Skin: Combination, Fair, Warm Hair: Black, Straight, Fine Eyes: Blue
Love the smell of this! Great!
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imgracekelly on 3/12/2013 12:58:00 PM more reviews by imgracekelly
Age: 19-24 Skin: Combination, Fair-Medium Hair: Brunette Eyes: Green
LOVE this bath bomb. It was the first one I used from Lush and I had a really awesome experience with it. I actually just repurchased it this week.
First I'll say I think it is the prettiest bath bomb in the store. I LOVE the smell. I kept sniffing myself after my bath because it made me smell so flowery and clean.
There were however, two things that I did not love about it. The flower on top is a very pretty touch...until it gets wet in the bath because then the petals float around your bath but don't dissolve....they just turn to soggy mush. The second part I did not like was the fact that my legs got itchy during the bath. I found that this happened when I used the Phoenix Rising bath bomb as well. I'm not sure if it's just me or if there is something in the bath bombs that cause this to happen to many people.
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sugarfreepersonality on 1/22/2013 10:51:00 PM more reviews by sugarfreepersonality
Age: 18 & Under Skin: Acne-prone, Fair, Cool Hair: Blond, Straight, Fine Eyes: Blue
This was my first bath bomb from Lush and I'm giving it a four for now- this is because I'm unable to compare it with other Lush bath bombs.
I enjoyed watching it fizz out in the water, and the dark pink color it turned the water. I love how pretty this looks dry, when you buy it in the store, as well. The water wasn't translucent, either, which is important to me- I'd rather it be somewhat cloudy. I used Sex Bomb in conjunction with the bath melt Ceridwen's Cauldron, though, so I'm not sure which made the water cloudy.
The flower in the middle is pretty when it pops out, initially, but does turn soggy rather quickly. I just fished it out and threw it away, so that wasn't too bad (though a few stray petals floated around the tub a little, which was annoying).
Otherwise, I really enjoy the scent- I'm not sure if I really get a strong hint of jasmine, but perhaps I'm used to more sharp notes of jasmine whereas this is muted and soft. Either way, I like this scent very much; it's been two days since I used this bath bomb and the scent still lingers in my bathroom!
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*TOP REVIEWER* blushie on 9/9/2012 10:01:00 AM more reviews by blushie
Age: 19-24 Skin: Sensitive, Fair Hair: Blond Eyes: Blue
Sex Bomb is a very feminine bathbomb...it smells like musky jasmine which is great on a girl, but maybe not on a guy if you're thinking of taking a bath together with this (in my opinion anyway!). It fizzes away quickly and zooms around on the bottom of the bath, making the bath a deep magenta. What I loved is that unlike some other Lush bathbombs, the scent didn't fizz away with the bomb and it's stayed on my skin. However this strong scent is what I think made my skin feel a little bit irritated during the bath. It's fine now, but I will probably cut the bomb up and just use half next time. The rose looks so pretty when it's dry, but turns into soggy, gelatinous clumps in the water, which I had to pull out of the drain at the end. This is only a minor issue though,I will buy this again.
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maeshiil on 7/27/2012 1:50:00 AM more reviews by maeshiil
Age: 18 & Under Skin: Acne-prone, Fair-Medium Hair: Brunette, Wavy, Coarse Eyes: Brown
This is my all-time favorite bath bomb. The scent is marvelous, the bomb itself is adorable and it leaves the bath water a beautiful shade of pink. :)
I'm obsessed with Jasmine, so this is perfect. ♥
Top Rated Bath Treatments
- 102 reviews
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Critic & User Reviews
posted by ninjaman9143 (MARRERO, LA) Sep 24, 2013
Member since Sep 2013
unique and worth it!
posted by aleksi (HIDDEN VALLEY LAKE, CA) Apr 27, 2013
Member since Apr 2010
A Brutal Victory of a Game
posted by ShadowHunter74 (BEND, OR) Mar 6, 2013
Member since Feb 2013
Brutal Legend
posted by FatPanda347 (BEAUMONT, TX) Feb 9, 2013
Member since Feb 2013
Very Good
A romp thru nostalgia-land
posted by TheSolly (COLORADO SPRINGS, CO) Jan 28, 2013
Member since May 2009
Jack Black found his calling as a cartoon. His performance in BL is arguably better than anything he's done in films. Here Black gets to run wild with his love of that time in music that came off as hammy in School of Rock. He simply OWNS this role.
A parade of real life rock stars voice virtually every main character, often making jokes at their own expense. The melding of music and the fantasy world shows great creativity, such as bass strings made from metal spider webs. This game was nothing if not a labor of love for someone.
The game's world is large and varied, but a bit sparse on things to do in it. Repetitive mini-games and easter eggs provide a reason to tool around and take in the sights, but you essentially do the same half dozen things outside of the main storyline. None of it is required, but at least they tend to be pretty fun.
The game attempts a radical departure into the RTS tower-defense genre, but generally fails. It's a creative take on an RTS battle, being a concert setting. But it's the least engaging and fun element in the entire game. Luckily you're forced to do it a very few times. It has some potential but is largely forgettable.
This is a game that I was left wishing there was just MORE of. Everything in it was fun, quirky, and mixed challenge with simplicity really well, and made me smile constantly.
People unfamiliar with the time Black is both mocking and worshipping may find better examples of each individual game element somewhere else- but if you're iPod already had Angel Witch and Celtic Frost on it- then trust me when I say this is a game you'll at least throw a couple devil horns to before you're done.
A favorite that I play as much for it's soundtrack than it's action.
posted by zerohour850 (PHOENIX, AZ) Jan 15, 2013
Member since Jan 2013
Awesome game!
posted by Emweekins8807 (MINNEAPOLIS, MN) Jul 19, 2012
Member since Jul 2012
posted by ShotgunShawn (FORT VALLEY, GA) Apr 2, 2012
Member since Mar 2012
Very Good
1 Of The Best games of all time
posted by TheTruth231 (MUSKEGON, MI) Feb 23, 2012
Member since Feb 2012
Silence ground walkers! Here is my review
posted by DanNaruto1 (CHANNAHON, IL) Feb 18, 2012
Member since Aug 2010
posted by elmattador (CIBOLO, TX) Feb 13, 2012
Member since Nov 2011
The gameplay, however, is solidly average. RTS and action games don't blend well IMO. Imagine playing a generic Devil May Cry action scene(with about 5% of the combos and waaay slower), but while you're slashing demons to bits, you have to command troops and capture strategic positions with them. It doesn't work well, although at least they tried something new. It's also incredibly repetitive. "Stage Battles"(the RTS parts) are always the same-Capture points on the map, call troops, advance forward, all the while hacking and slashing. The sides missions are repeated over and over- ambush these guys, shoot those guys, beat this guy in a race.
Still, I kind of recommend you rent this just to experience the environment; it's unlike anything else I've played. Sadly, the gameplay just doesn't hold up.
I had hope for this.....
posted by denzo45 (ASHTABULA, OH) Jan 1, 2012
Member since Sep 2011
Awesome Soundtrack
Variety of Baddies
Deuce (car) drives like a brick, terrible handling
Main character can't jump....
Constantly getting stuck on objects
No HUD or Health bar
No map on the screen, had to constantly hit pause to look at the map
I forced myself to finish this game and immediately put it back in the mail. I didn't know whether this was a hack/slash game, or car combat, or some kind of pseudo-RTS, but it was just too weird. The game is an open world, where you go driving around in your car, and go to contacts for missions, then on almost half of your missions you are commanding an army and building units to battle the enemy army (think Command and Conquer). I thought it would be fun but I think it was just too much going on.
posted by mamajama72 (PLAINFIELD, IL) Nov 7, 2011
Member since Oct 2011
Great game, but for a specific crowd
posted by AwwMang (TWO RIVERS, WI) Oct 20, 2011
Member since Dec 2010
First game I sent back in one day.
posted by bobthe23rd (STATE COLLEGE, PA) Oct 18, 2011
Member since Mar 2011
Heavy Metal has a voice and this game is it.
posted by TorikoX (HOPE, AR) Sep 1, 2011
Member since Aug 2011
Really Bad
posted by matthew13 (CHESWICK, PA) Aug 18, 2011
Member since Jul 2010
Greatest Rock/METAL game since Guitar Hero
posted by iSpartan926 (BROOKLYN, NY) Jul 22, 2011
Member since Jul 2011
epic and funny
posted by mte46460 (MENIFEE, CA) Jul 15, 2011
Member since Jul 2011
Good Story
posted by TweakerG (SHELLEY, ID) Jun 23, 2011
Member since Nov 2009
Almost Too Much "Awesome!!!"
posted by Kriminator (CROSBY, ND) May 23, 2011
Member since Aug 2010
4 out of 6 gamers (67%) found this review helpful
If your around 35-40 ish and grew up listening to the likes of Ozzy. This is worth your time.
Note: unlock a bunch of stuff before you start the first Tour of destruction, get all leveled up with unlockables in the northern part of the map, then you should be fine in real battles. Do it Not! and perrish in the wake cryptic characters that look like "Beatle Juice"3... Dont actually say beatle juice 3 time tho... LOL
Awesome, but frustrating.
posted by Trisphere (BRATTLEBORO, VT) Mar 19, 2011
Member since Mar 2011
I like the diversity of game play...at first it seems to be a hack n' slash type game, but then they throw in a car known as the "Druid Plow" which introduces elements of driving games. Then there are the "stage battles" which are basically real time strategy games where you produce and control various units such has "head-bangers" or "roadies" and attempt to take down the enemy's stage.
My main beef was that the game wasn't entirely consistent with the level of difficulty. A lot of it seemed far too easy, (though still very fun to play,) but once in a while there would be a part that seemed nearly impossible! I nearly stopped playing on the second to last battle because I got so frustrated. I don't mind a challenge, but the stage battles are kind of tedious at times, and it wasn't very fun having to go through it over and over again, only to fail each time. I was able to win the game, only with a bit of help from the internet.
I still recommend the game, though. Overall it was fun to play, and had plenty of laughs.
Brutally AWESOME!
posted by happythug (VANCOUVER, WA) Feb 1, 2011
Member since Oct 2007
5 out of 7 gamers (71%) found this review helpful
Graphics: Detailed, stylized, UNIQUE, varied and awe inspiring environments that I actually stopped to look at several times. Who thought a metal-themed-world could look so fascinating and beautiful.
Gameplay: Brutal Legend mixes elements from strategy, racing, action, open-world and RPGs flawlessly. Each form of gameplay is fun. Combat is always entertaining and fun to look at, and the game keeps giving you enough upgrades to keep things interesting. Avid explorers will be well-rewarded, as finding collectables is key to earning certain useful upgrades. Even fetching collectables is fun because you don't simply walk up to the floating-spinning-obtainable like in most games. Collectables are usually obtained by playing a short-song at a place or object where a tell-tale sign of goodies alerts you to the presence of something useful. Maybe it's the red-thorn flowers around an old monument, or maybe a dragon statue bound in chains. They are always easily identifiable, and not simply collected, but unleashed. Idols raise from the ground with sparks and lights. Dragon Statues scream fire and break their chains. Just driving around the countryside looking for things is fun in this game.
Story/Setting: Characters are humorous, unique and well acted. So many bazaar concepts are well imagined and brought to life by Brutal Legend. It took me totally off guard because I was really expecting some dank, dark and offensive metal-setting, and what I got was a colorful and beautiful fantasy/metal world. The story does a good job at motivating you to keep moving forward producing an adequate amount of "care" for its characters and events.
That's all the room I have for this review, but I encourage even the most skeptic of gamers to give this game a shot.
rock on
posted by HolyOwl (DULUTH, MN) Dec 31, 2010
Member since Dec 2010
Brutal Legend Review by Xenization
posted by Xenization (WEST LAFAYETTE, IN) Dec 15, 2010
Member since Dec 2010
The game itself is actually really good. Graphically the game is well above the standard you would expect from a EA push out. The animations are flawlessly done, and the animators clearly had a fun time with this game. The demons look moderately grotesque to fit in perfectly with the incredibly disproportionate human characters. But all the character designs were done with a purpose so it never seems too difficult to imagine what each character is going to do for or against you. The major influence behind this game, Jack Black, is the main character, and you can see his style smashed all over the game. There are countless little jokes and innuendos set right into almost every cut scene. Overall the graphics and audio were better than expected in this game.
The storyline is EXTREMELY short. If this takes you more than a couple days to finish then you are failing the missions way too often. Personally I took the "Brutal" difficulty to town in around 2 days. FYI "Brutal" is not actually that brutal. My only wish is that they would have been able to stretch the story out a little bit and keep the comedy going. The ending (don't worry I won't spoil it) is well set up, but it's definitely one of those you can see from a mile away. If you've ever seen an M. Night Shyamalan (don't worry ... even I had to look up how to spell his name) movie this one will not phase you.
All in all this game is a solid rental, but not anything more.

subset: commoncrawl


text:
Debrahlee Lorenzana Is Suing People Again
This time, a nurse at blood-draw center and a taxi driver.
Skip to content, or skip to search.
Skip to content, or skip to search.
This time, a nurse at blood-draw center and a taxi driver.
"Limp handshakes" may or may not be one of them.
"She hasn't changed a thing about the form-fitting outfits or high heels that she says bosses told her to quit wearing."
Justice for the woman who described herself as "Tits on a Stick."
A slave collar is involved.
Another development we would have never predicted!
Debrahlee Lorenzana's epsiode of 'Plastic Surgery New York Style' will air this weekend.
We all have dreams.
"If Chase fires her, I will stop banking with them," says high-profile customer.
The banker turned advocate for good-looking people's rights speaks out.
If she doesn't stop talking about how hot she is, the former Citigroup banker may be fired from her new gig.
Her beauty actually WAS distracting.
The woman who claims she was fired from Citigroup for being too good-looking continues to fight against the uglies who try to keep her down.
A former Citigroup employee claims she was fired for being too darn hot.


text:
Didn't find what you were looking for? Search here for more!
(Mali ba? Hindi ba ito gusto mo makita? I-type at hanapin dito!)
Nipa Hut Restaurant at Oranbo – Pasig
Have you ever tried out this restaurant call NIPA HUT located at Pasig? They have a great place there which I am sure you'd appreciate specially if you are the kind of person who enjoy a simple get together at night or perhaps you want to experience a "Tagaytay-like" view even without going to Cavite. If you haven't tried it yet then this post will definitely clear some things out.
I was only able to experience this place about 3 weeks ago… ahm not really sure though. Haha sorry for the late post. Anyway… lets answer some FAQ
So where is this located?
The Nipa Hut Restaurant is located at Capt. H.P. Javier St. Oranbo, Pasig City. So where exactly is it? When I asked how to go there, they told me to its near Ultra and/or DepEd. Well click this MAP to view Oranbo, Pasig
Upon arrival, you will see the following sign. The parking lot is also the front entrance.
Also you will see this sign.
So what makes the place special?
The place or the restaurant is located on what I call a "tagaytay-like" view. They have a nice panoramic view of the city of Pasig. Its ok during the morning (I guess because have not been there during the day) , but I really love the view during night time.
there is Pasig at the background
what seemed to be a badminton court at the background (again)
they have a big space, which looked like a backyard garden to me
here is a portion of the stage
some more plants
this one looked like a big Gazebo
path going to the main entrance
view going out the main entrance
the crew
and the utensils located just outside
I am glad that when we went there, a live band was performing. I always love live performances… In fact, the keyboard malfunctioned a couple of times when we were listening. LOL
So what do they serve?
BEERS! haha of course a restaurant like this one would definitely have beers!
If you want super cold beer. Well, just so you know, when we ordered a bucket of regular beer there, it was SO cold that we thought we were served BEER BELOW ZERO. They have what they call CRAZY HOUR when beer prices drops significantly.
CRISPY PATA (it that big or is it just me?)
If you want to see more, you can check out their menu below.
for more info you can visit and become a fan of Nipa Hut's Facebook page here. more PICTURES here at this NIPA HUT album
Take a break from work…Dine and Drink under the stars and enjoy the Panoramic View that Nipa Hut offers!
Capt. H.P. Javier St. Oranbo, Pasig City
We Accept Reservations and Catering.
Contact us: (632) 671-5321
Telefax: (632) 671-5350
Related Posts :


text:
NCSL's election experts break down the midterm state legislative elections along voters' verdict on statewide ballot measures.
Current Issue
Minneapolis—About 150 state legislators and staff will promote fitness and healthy living by participating in a 5K fun run/walk in Minneapolis as part of the National Conference of State Legislatures' (NCSL) Legislative Summit, Aug. 19-22.
The Walk for Wellness begins at 6:30 a.m. Thursday, Aug. 21, at Loring Park, the largest in central Minneapolis, near the 14th Street entrance at Willow.
This is the ninth annual NCSL Walk for Wellness. The event was started by a coalition of legislative caucuses to offer an opportunity for exercise during a long week of meetings. It is designed to raise awareness of the adverse effects of heart disease, diabetes and obesity.
Since 1975, NCSL has served as the bipartisan organization serving the legislators and staffs of the states, commonwealths and territories. It provides research, technical assistance and opportunities for policymakers to exchange ideas on the most pressing state issues and is an effective and respected advocate for the interests of the states in the American federal system.
7700 East First Place
Denver, CO 80230
Tel: 303-364-7700 | Fax: 303-364-7800
444 North Capitol Street, N.W., Suite 515
Washington, D.C. 20001
Tel: 202-624-5400 | Fax: 202-737-1069


text:
Manteca High School Alumni
Jim Wolfe
Class of 1962
Ronald Shults
Class of 1979
Kelly Gibbs
Class of 1993
Stacey Van Riet
Class of 1984
Alumni from Manteca High can use this site to get in touch with old friends from their high school in CA. For former Manteca High students from CA, you can view the class reunion information that has been posted by other site visitors. In addition, we encourage you to post your profile in the alumni directory, share information about the school activities you were involved with and look for lost friends. If you haven't already, we also encourage you to register for free on this site. You may also want to check out the latest news and school memories.


text:
Ojos Que No Ven, Corazon Que No Siente
Translation: What the eyes don't see, the heart can't feel. Or, what your eyes can't see, can't hurt your heart.
Or, is it more out of sight, out of mind?
This dicho, this frase, always has baffled me.
On the one hand, what you don't see, don't know, can't hurt you. True.
But, it is an old call to ignore — purposely– something that is wrong? Something hurtful? We as a culture of women are too often still called upon to turn our eyes away from truths. Para no sentir lo malo. So we don't have to deal. To aguantar, if you will.
Or, is it an act of survival, of lo que sea, of moving forward without drama and trauma? Why rock the boat?
I have purposely averted my eyes before…in all manner of things. Sometimes, you have to, and sometimes, you just don't know what else to do.
I wonder, when I hear this frase, or when it pops into my head, if I ever will use it on my daughter.
And, I guess it depends. Depende.
Is it love, work, friendship that has her hurting, doubting? How badly?
But, in the end, esta believes that seeing truths is way better than not. Because, the heart heals. No matter what.
Ojos Que No Ven Video by Alexis y Fido
I do love how this dicho is so popular, so forever relevant…even in Reggaeton. Check out Ojos Que No Ven.


text:
Be sure to stop by the Smith Mansion Store!!
(ALL proceeds benefit the preservation)
SMPP Statistics
As of September 20, 2013,
The Smith Mansion Website has had an astonishing 8,793,988 hits to our site!
SMPP also has followers and supporters
from over 40 Countries, and counting!!
Become a Supporter Today!
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text:
Man charged in year-old murder at New Castle truck stop
A 21-year-old man faces charges in connection with a murder at a New Castle truck stop last year.
State Police say Larry Bailey of New Castle fatally shot 56-year-old Ronald Hardy in the chest last April during a robbery.
Hardy's body was left at the scene at the Delaware Truck Plaza on South DuPont Highway.
His accomplice, Carrie Webb of New Castle, plead guilty to robbery charges in March and is awaiting sentencing.
Bailey is being held without bail on first-degree murder charges.
Copyright © Dec 19, 2014, WDEL/Delmarva Broadcasting Company. All Rights Reserved.
Advanced permission from Delmarva Broadcasting Company required for publication or rebroadcast.


text:
New Music Video From Chris Young 'Tomorrow'
April 28, 2011 5:48 PM
Chris Young's music video has been released for his new single, 'Tomorrow'. The music video features Young portrayng a man who is trying to leave a relationship, but struggles with the choice to walk away.
"Tomorrow" will be the lead single off of Chris's album, Neon, scheduled to be released this summer.
[photogallerylink id=25411 align=left]


text:
Ok, so let's analyze this one
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Ring of Famer
Join Date: Jan 2006
Location: Phoenix, AZ
Posts: 10,756
Chris Harris
Originally Posted by
I can't be the only one who has absolutely no problem with this. It very nearly worked against the Giants and it gives them at least a shot to win the game. It ain't over until the clock hits zero after all. I never understood why you just have to accept the outcome when the other team takes a knee.
Wrong - it did NOT nearly work against the Giants. Who cares if the QB falls down because of the surge. The center to QB snap is over with in less then a second. The defense has absolutely NO chance at causing a fumble, especially if you make it a weekly thing and teams know that you're going to try that crap. The QB has the ball securely in in hand before the defense even reacts to the snap. There is a reason why why it has become normal in the NFL for the kneel down and the defense respecting it. The ONLY thing that will happen is injuries. I seriously hope the lose somebody to an injury while doing this and then maybe that lame ass HC will learn something about the NFL.
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Join us for a fun-filled family event! For kids, we will have a moon bounce, video game competitions, face painting, and board games. Adults can participate in hand and line dancing demonstrations, chess and card game competitions, "Shaw Community's Got Talent" showcase, and Zumba sessions. You can also engage in other activities geared toward improving your health, such as free health screenings and talk to presenters about immunizations, diabetes, Alzheimer's, HIV/AIDS, stroke, arthritis, heart disease, hearing, opthalmology, podiatry, and much more.
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City to observe holiday
The city of Ennis will close its offices and refuse collection routes on Tuesday, Jan. 1, in observance of the New Year holiday. The city will reopen its offices and garbage collection routes will resume as normally scheduled on Wednesday, Dec. 2.
Posted by publisher on Dec 29 2012. Filed under Community calendar. You can follow any responses to this entry through the RSS 2.0. Both comments and pings are currently closed.


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The official thrift/discount store bragging thread - Page 4035
Styleforum Top Picks
- Rolex Oyster Perpetual Datejust Mens Watch 116234No reviews yet.
- Frederique Constant Classic Chronograph Watch - FC-291MC2R6No reviews yet.
Good morning SF!
Yes, they are available. I'll put them up in my store in a few days. Sizes are 42 44.
It has that tag because when Spoo was doing the charity thing during Christmas I wanted to contribute.
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Wait. So you bought something from Spoo to resell?
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Those look similar to the gold buttons I found (see below), mine (award winning) were marked Lindsay 14k and I was able to find the on-line catalog for the company. What's the name on the back?
Edited by lastdonb - 1/18/13 at 9:57am
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Southwick 40S Three Piece Tweedy Check Suit
Warning: Spoiler! (Click to show)
I'm really torn on this one as it fits (though it needs a little bit of tailoring because of my massive thighs) but I don't know that it's anything I'd ever really wear.
J&M Wholecuts 10D-
Warning: Spoiler! (Click to show)
Willis & Geiger Shirt M-
Warning: Spoiler! (Click to show)
More SW&D I think- Troglodyte Humonculus-
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Gucci Belt- tagged a 42 but cut down to a 34ish
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Are those GOLD BUTTONS!!!!!!!!!!??????
If there's one thing that gold button thing has me doing, it's checking buttons that look like they might be gold... I have found some interesting blazers that way -the better ones seem to have buttons that are closer to real gold in color. perhaps gold plated?
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Lurk longer. Dior ties are kind of an inside joke.
Don't mean to bust your stones, dude; I'll give you the benefit of the doubt since it says "Cravate" not "Homme," but I'm pretty sure that's not the Dior you're looking for.
BTW, Tubs: I think Cap is right. I think Mex started a spreadsheet like the one you suggest, and it ended up kind of petering out.
thanks for the welcome? but thanks ill know better next time
Oh, sorry, dude! We usually prepare the following welcome spread for each and every new member. Here you go:
Just an internet meme.
Styleforum Top Picks
- Seiko MECHANICAL SARB035 Mens Wrist WatchNo reviews yet.
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text:
46, West Springfield, PAI want to find a lady that will hold my hand.go to church with me.talk to me.have fun together.since my divorce four years ago .I have had troubles going constantly to church....
56, West Springfield, PAi love the outdoors-beach, mountains, woods. i enjoy hot summer days and all seasons. i like many forms of art, music, and books. i have had the same job for 31 years. i work ...
57, West Springfield, PAI am looking for a lady that wants to spend time with and share her life with the man she loves. I am a romantic, I love to hold hands, put my arms around you, and respect you...
48, Lake City, PAI like to work hard and play hard, I am one to get things done, my friends say I am a lot of fun. I like to experience life, try new things, I feel I am romantic and sensitive...
44, Girard, PAI'm interested in meeting a girl who I can spend time with and get to know. I have never been married. I have a beautiful little daughter who is the reason I am back to church...
63, Girard, PAHi, we'll this is the hard part. I don't like to talk about myself so I'll keep it simple and we can talk on phone or in person. Iam a very easy going person that enjoys simpl...
42, Lake City, PAOccupation: Other; Height: 5' 11"; Eye Color: Blue; Hair Color: Brown; Political Views: Unknown
49, Fairview, PAI love life. Work hard at my job and around home. I'am a handy person. Like to meet a lady to share my time with. Like most music no hard rock or rap.Have interest in sports....
52, Fairview, PAI would like to meet a girl who is interested in joining together on our Faith Journey. I am young at heart and would like my match to be spiritually and emotionally healthy, ...
68, Fairview, PAWhat I bring to the table: I listen deeply to your thoughts and feelings. I have a compassionate heart. I am patient . I will inspire you to greatness and help you bring your ...
29, Fairview, PAI enjoy playing golf, reading books, drinking beer, and making people laugh. I'm very passionate about giving to people in need and enjoying each and every day.
66, Fairview, PAOccupation: Other; Height: 5' 9"; Eye Color: Blue; Hair Color: Salt-n-Pepper; Political Views: Unknown<...
Browse By City:
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text:
2014 BOLA Club Bowling Machine
The 2014 BOLA Professional Bowling Machine
Fully Enclosed Cricket Bowling Machine. Concave profile moulded urethane ball delivery wheels. Microprocessor based digital speed control with membrane switch panel. Adjustable speed in increments between 15 and 95 mph. Spin, swing and pace bowling with 19 spin and swing settings. Electronic random variation facility with solid state electronic wheel braking. Ball joint complimented by vernier screw adjustment for precision line and length. Operation from 12 volt battery or with optional BOLA Power Pack from 230v mains (or 110v if specified). More...
ex VAT. £2038.00 inc VAT
The 2014 'Merlyn by BOLA' Spin Bowling Machine
The 'Merlyn by BOLA' spin bowling machine – which was developed by Stuart & Williams (BOLA) in conjunction with the England and Wales Cricket Board – was greeted with praise and enthusiasm by professional players and coaches alike when it first went on sale last year. The ECB were searching for a means to extend the experience of first-class players against top class spin bowling and after receiving a design proposal and viewing a mechanical prototype twenty machines were ordered which are currently in use at every first class county, the England test squad and the ECB Academy.'Merlyn by BOLA' provides the coach with the flexibility to deliver any combination of leg-breaks, off-breaks, top spinners and arm balls that he or she requires. The 'easy to use' switch panel allows complete control of line, speed, flight and the amount of top spin and side spin imparted to each delivery. The machine can be programmed to deliver any 'over' the coach can conceive. More...
ex VAT. £12,900.00 inc VAT
The 2014 BOLA Ball Trainer - only available from this website!
The 2014 BOLA Ball Trainer is a unique design concept, specifically developed to produce the consistent, accurate supply of ball delivery that is essential to teach youngsters a plethora of hitting, catching, fielding or striking skills. The Ball Trainer is supplied with the battery, a mains power pack/charger, 12 cricket practice balls and a novel tennis ball catching deflector for perfecting catching or volleying skills. More ...
ex VAT. £1800.00 inc VAT
The 2014 BOLA Hockey Ball Delivery Machine
A floor mounted version of the cricket machine with enclosed wheels. Delivering a hockey ball at speeds from 20 to 80 mph. Vernier length control allows fine adjustment to eliminate bounce. 12 volt battery operated with mains option available. Also available is a kit to allow conversion for cricket use. Supplied in carrying case. More...
ex VAT. £1884.00 inc VAT
BOLA 2014 Fielding Trainer with Gimballed Turntable
BOLA Hockey Ball Machine mounted on a gimballed swivelling turntable.
Instant adjustment of direction and elevation. Designed for Hockey goalkeeper
training the turntable is excellent for a wide range of Cricket fielding
and catching exercises. Availlable with extended legs.
ex VAT. £2232.00 inc VAT
The 2014 BOLA Baseball Pitching Machine
Pitching Machine with enclosed wheels for user safety and comfort. Light weight for portability and fast set up. New urethane ball throwing wheels for extreme accuracy and longevity. Custom Digital Microprocessor speed control with 1mph increments from 11 to 95mph. Digital ball speed display. Ball joint adjustment and vernier (screw) adjusters for positive fine pinpointing of height and width. 12 volt battery operated with mains option. Supplied in carrying case. More...
ex VAT. £2038.00 inc VAT
BOLA Baseball Machine is packed in one box (dimensions 92 x 56 x 42 cms)
and the legs are packed
BOLA Power Pack (Mains option)
A 240v (or 110v optional) high amperage transformer giving a smooth DC supply for BOLA Bowling Machines. This enables the BOLA to be used from a mains outlet. Circuitry also includes a built in battery charger for overnight charging of batteries.
ex VAT. £281.00 inc VAT
The 2014 BOLA 28 Ball Automatic Feeder with Remote Control
The BOLA Automatic Ball Feeder will fit both the 'Club' and 'Professional' models. It can be attached in less than 2 minutes and feeds a ball into the machine every 7 or 11 seconds (as selected). Optional remote control on/off switch allows interruption of the sequence when required. An ultrabright LED indicator and a beeper warn of the release of a ball. The ball feeder offers a unique solo batting practice facility or the option to coach from any position. An Electronic Random Delivery Mode is also available for the 'Professional' model. More...
ex VAT. £417.00 inc VAT
Practice Balls
5oz Practice Balls. Polyurethane practice balls in red or yellow with
soft case to protect bats from damage. Dimpled like a golf ball for stable
flight. These give the best accuracy and realistic bounce. Yellow balls
for better visibility indoors. Red for realism.
ex VAT. £51.41 inc VAT. per doz.
3oz 'Orange' Practice Balls. The Orange ball is designed to give higher
bounce to enable players to practice back foot shots at reduced ball speeds.
Players can play hooks and pulls safe in the knowledge that the softer
ball will not cause injury. Also used for junior age groups to provide
bounce when the BOLA is on it's shorter legs.
ex VAT. £51.41 inc VAT. per doz.
BOLA Practice Ball Bag
The BOLA Practice Ball Bag is the perfect accessory for collecting and storing your practice balls. Order four dozen our more balls through our Online Shop and receive a FREE BOLA Ball Bag!
ex VAT. £10.80 inc VAT
12 Volt Sealed Gel Battery
Ampere Hour Totally Sealed Leisure Battery incorporating the latest AGM
and GEL Technology. No spillage, completely maintenance free and easy
to transport safely. Quality BS EN ISO 9001; 2000. This battery will give
up to four hours of machine use and is designed for deep cycle applications.
ex VAT. £123.60 inc VAT
BOLA Machine Stand on wheels
Accepts any BOLA machine with no modification required. It enables the machine to be easily wheeled around without dismanteling and so maximises the time in use. It provides the Machine operator with a safe and stable position.
ex VAT. £708.00 inc VAT (plus delivery charge)
BOLA Machine Compact Stand
A compact BOLA machine stand on 4 casters (needs a flat surface to stand on). Designed for smaller storage spaces with a reduced footprint (900mm x 1000mm). Movable height machine bracket for adjustment of ball delivery height – 4 bolts and wing-nuts. Operator protection for lower body. Easy and quick to move and set up. Will accommodate all BOLA Bowling Machines and Auto Feeders.
ex VAT. £486.00 inc VAT (plus delivery charge)
Set of short BOLA Legs with rubber feet
ex VAT. £52.80 inc VAT
Super-Tuf plastic stump set
ex VAT. £33.90 inc VAT
Multi Stage 12v Battery Charger
ex VAT. £49.20 inc VAT
|email email@example.com
All Machines are carriage free (UK Mainland only). Please ask for a quotation to any overseas destination. A carriage charge (minimum £9.00 VAT) is payable for all balls and accessories. The carriage charge for a battery is £20 plus VAT - unless it is included with a machine order when it is free. Carriage is payable on machine stands.
A battery is NOT included in any machine price.
|terms & conditions


text:
Item #: 2252847
Shipping Restrictions: This item cannot be shipped via air.
REVIEW SNAPSHOT®by PowerReviews
(based on 1 review)
Reviewed by 1 customer
(2 of 2 customers found this review helpful)
Great For Guitar Players
Best Uses
Comments about Sally Hansen Salon Gel Polish Basic Refill Kit:
This should be in every guitar player's arsenal. Easier to apply than acrylics and just as strong.
I'm a male guitar player and have used fake nails on one hand for fingerpicking for a long time due to my relatively weak natural nails. I got tired of going to a salon all the time so I learned to do my own. This is easy enough to apply at home and I can touch up my nails any time I need to.
For my guitar manicure I use two coats of each the base and top coat instead of just one. This allows me to build up my nails and make them strong enough to endure the repeated punishment dealt to them by the strings of the guitar. They sound great and are discreet enough so that nobody notices them unless I point them out.
It does take a bit of practice and patience to apply, and depending on how much you punish your nails they can start to lift around the edges. Of course, just tack them down with some thin nail glue or krazy glue if they start pulling up and you're good to go again.
Strong, simple, and much more cost effective than going to the nail salon every two weeks.
Bottom Line Yes, I would recommend this to a friend
(2 of 2 customers found this review helpful)
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The Panther: Cartier's '30s Muse Inspires a New Collection
Jeanne Toussaint serves as its namesake.
Skip to content, or skip to search.
Skip to content, or skip to search.
Jeanne Toussaint serves as its namesake.
Along with her latest collaborator, the designer J.W. Anderson.
Mark your calendars for May 22.
Exclusive looks by Missoni, Helmut Lang, Proenza, and more.
She might not be well, but she's back!
We hate to say it, but they are underwhelming.
The designer will create a capsule men's and women's collection for the label for next spring.
Also, Preen launches a new capsule collection and Marchesa's Georgina Chapman turns to jewels.


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text:
Foxes fightback saves Sven`s record
Leicester staged a dramatic second-half comeback to earn a point against in-form Leeds in a 2-2 draw at the Walkers Stadium.It looked like Sven-Goran Eriksson would suffer his first home league defeat as Leicester boss when his side went two goals behind.Curtis Davies and Kyle Naughton were caught napping at the back and a slip from the latter allowed former Fox Max Gradel to head home Neil Kilkenny's right-wing cross after 19 minutes.Robert Snodgrass extended Leeds` lead with an extraordinary strike 10 minutes into the second half. Snodgrass` corner was headed back to him and the midfielder hit a first-time shot with the outside of his foot from the narrowest of angles that flew past Chris Kirkland.Leeds were in command, but the game changed in the 72nd minute when they surrendered possession in midfield and Darius Vassell raced through on goal. The striker was brought down by Kasper Schmeichel, who was lucky to escape with just a yellow card, and Paul Gallacher scored from the penalty spot. Four minutes later and Leicester were level. Andy King controlled a ball over the top and smashed a 25-yard shot in off the underside of the crossbar.Leeds had two chances to snatch victory late on, both from corners. Kirkland saved from Neill Collins before the ball was hacked clear and Billy Paynter also had a shot cleared off the line.
Sponsored links
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text:
Joined: Apr 26 2008
OrangeWeaponsI'm doubting it.
Jan 27 2011, 10:22 am
CauTi0NHey dude, I was really hoping you would make a game soon. :/
Jan 7 2011, 7:48 am
Kevin208I play LoL alot xD, I PWN With Heimerdinger and Fiddlesticks, newbie with Kennen, IGN, Super Sayajin Level 11 Add Me! Page me Your IGN!!!
Nov 5 2010, 9:40 pm
GamemakingdudeOrangeWeapons wrote:
Oct 12 2010, 2:05 pm
I don't see any games being made, dude.
Spy wars is an game i already made.
OrangeWeaponsI don't see any games being made, dude.
Oct 12 2010, 2:04 pm
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text:
Florensia is a 3D next-generation free-to-play anime fantasy Massively Multiplayer Online Role-Playing Game (MMORPG) from Japan. You can download the client for free and play at no cost. In the game, you will meet thousands of other players - play together with people from all over the world, make new friends and become a part of the international Florensia community.
The following overview will guide you through the registration, download and installation process of Florensia.
|Step 1 - Account creation
In order to play Florensia, you have to register first. The registration is completely free - all you need is a valid e-mail address. No payment information have to be submitted.
Click here to create your free account for Florensia!
|Step 2 - Account confirmation
Once the registration is completed, you will receive a confirmation e-mail to validate your registration. Follow the instructions in the e-mail to activate your account.
After your first login to the website, your registration is complete.
|Step 3 - Game Download
You have to download and install the Florensia game client to play Florensia. The client can be downloaded for free from various download partners.
Click here to download the Florensia client!
Please make sure your computer meets the system requirements for Florensia. You can optimize the graphical settings in the game later on.
You can check the system requirements here
|Step 4 - Installation
Once you have downloaded the client, run the FlorensiaInstall.exe file and install the game to the desired folder.
Click here to read our detailed Installation guide
|Step 5 - Patch Download
Double-click the Florensia icon on your desktop to start the Florensia launcher. The launcher will automatically update (patch) your client to the latest version.
Choose the language you want to play Florensia in and start the game when the patching process is completed.
|Step 6 - Character Creation
Log in to Florensia and choose the server you want to play on. When you connect to the server, you will be able to create up to five different characters.
Choose your job class, gender and looks and join the world of Florensia in the town of Roxbury.
|Step 7 - Start Your Own Adventure!
Create your own profile on the website and talk about Florensia in our forum.
Learn more about the game, make friends to achieve your goals. Join a guild, create your own warship and visit the website frequently to get the latest news around the game in time!


text:
Welcome to Fashiolista!
Discover, save & share the greatest fashion finds
from anywhere on the web!
from anywhere on the web!
WIZARD of OZ Necklace - Dorothy's Ruby Slippers - You've Always Had The Power. One side has a picture of Dorothy from the MGM movie The Wizard of Oz. collaged with a vintage dictionary page that says "Home." An adorable pair of red glittered shoes hang from the bottom adding a touch of whimsy to this necklace. Ruby color glass Czech beads add a bit of sparkle. The back of the pendant reads, "You've always had the power," reminding us of when Glinda told Dorothy. "You've always had the power to go home."


text:
Professor Reid published in Muséologies, Visual Arts Research and presented at Pedagogy conference
Degrees Offered
MFA Alumnus Jonny Black and business partner launch new portfolio site - castirondesign.com
UANews.org: Professor Ellen McMahon featured
Professor Sheila Pitt awarded at the Univ. of Maryland's Drawing and Print Competitive Exhibition
Professor Frank Gohlke's work featured in SiteSantaFe Exhibition
jrn.com: Professor David Taylor featured in news report
Professor Ellen McMahon article in Huffington Post + PSMAG.com
Art & Visual Culture Education Faculty Kudos!
Book Art Collective meeting first Wednesdays at 5:30pm
MFA Student, Kitagawa, given an ISC achievement award
Prof. Alshaibi awarded Non-Fiction prize by Arab American National Museum
Photography Professor, David Taylor's 1821 Border Project Underway
PhD student, Mary Statzer, quoted in New York Times, "Using a Camera to Make More Than Memories"
3D & Extended Media Facilities
Graduate Facilities
Burnaway.org: Gary Setzer's Tour of the Black Tongue
School of Art Professor, Dr. Widdifield co-awarded prestigious ACLS Fellowship
Photo Professor, David Taylor participating in international exhibition "Border Cultures"
University of Arizona
Student Work
Art Building
University of Arizona Museum of Art (UAMA)
Art Advisory Board
Program Requirements
Undergraduate Studies
Studio Art
Photography Plan of Study
Programs of Study
Graduate Studios
UA Student Union Memorial Center
Ceramic Annex
The Loft Cinema
Joseph Gross Gallery
Lionel Rombach Gallery
Graduate Gallery
Confluence Center
Letterpress & Book Art Lab
The Graduate Gallery
Center for Creative Photography
First Year Experience
Lionel Rombach Gallery
School of Art Curriculum Procedures
Joseph Gross Gallery
News & Events
MFA Catalogs
Faculty Catalogs
Outreach Activities
Painting and Drawing
MFA Thesis Archive
Copyright Information
Digital Library
Slide Collection
Services and Equipment
Graduate Studios
Studios and Classrooms
Digital Print Studio
Book Art and Letterpress Lab
Visual Resource Center
D2L (Desire To Learn)
D2L is a campus-wide, web-based course management system designed to create a rich online learning environment for students. D2L includes features such as online syllabi, an electronic drop-box, automated grade book, discussion boards, and quiz capability.
► Contact an Academic Advisor for more information.
Career Development
The Career Services Office is located on the fourth floor of the Student Union. This office can help you decide on a career, find an internship, practice interview skills, hone resume writing skills, search for employment and more.
►Contact an Academic Advisor for more information.
Student Organizations
For information about these groups, please call 621-1251 or contact a Faculty Member in your area of interest.
American Institute of Graphic Arts (AIGA) student chapter
Visit the AIGA website
Art Clayworks - undergraduate students
Visit us on Facebook
Art History Club - undergraduate students
Contact an Art History faculty member to find out more
Art History Graduate Student Association (AHGSA)
Visit the AHGSA website or visit us on Facebook
Book Art Collective
Visit our website or visit us on Facebook
Ceramic Alliance - graduate students
Contact a 3D faculty member to find out more
National Art Education Association (NAEA) student chapter
Visit us on Facebook
Persona Magazine
Visit us on Facebook
Photo Club
Contact a Photo faculty member to find out more
Viscom Club
Visit us on Facebook
Wildcat Print Association
Visit us on Facebook
University-wide Student Clubs and Organizations can be searched through ASUA.arizona.edu.
Art List Serves
The list serve provides information about advising issues, important deadlines, darkroom hours, portfolio deadlines, events, and student and faculty openings and performances. Subscribe to one of the listserv addresses below. Choose which listserv by your area(s) of interest or major:
Major/Area of Emphasis
|Art History
|Art and Visual Culture Education
|2D Studies
|3D Studies
|Visual Communication
How to Subscribe
- Send an email to email@example.com
- Write in the subject line of your email:
subscribe <space> name of listserv <space> Your Full Name
Note: Leave the message body blank. Delete or turn off any automatic signature.
See http://uits.arizona.edu/services/email/email-lists/help/subscribe for further instructions.
How To Unsubscribe
- Send an email to email@example.com
- Write in the subject line of your email:
unsubscribe <space> name of listserv
Note: Leave the message body blank. Delete or turn off any automatic signature.
See http://uits.arizona.edu/services/email/email-lists/help/subscribe for further instructions.
Problems? Questions?
Email an advisor:
Ashley Rubin, firstname.lastname@example.com
Daryl Flores, email@example.com
Or visit http://uits.arizona.edu/services/email/email-lists/help/subscribe for further instructions.
Art Opportunities
Please visit the Art Opportunities page often for information about outside exhibitions and calls to artists, events, jobs, internships, and scholarships and grants:
Undergraduate Tuition Waivers
The School of Art offers tuition waivers based on need and/or merit to students in good academic standing. Students must be majoring within a study area from the School (Art History, Studio Art, Art and Visual Culture Education) and are selected based on faculty recommendations.
To be eligible for tuition waivers, students must:
- Fall within the eligibility period (8 semesters at the UA)
- Have and maintain a 3.0 GPA
- Have US citizenship
- Enroll in 12 or more units, and complete 30 units per year
Applicants for need-based waivers must fill out the Free Application for Federal Student Aid (FAFSA) forms, available on-line or through the Office of Student Financial Aid. Please note that tuition waivers cover only a portion of tuition. They do not cover other University fees.
Student Resources
This section provides information about jobs, internships, call to artists, and outside scholarships and grants.
We encourage all majors and minors to get involved with School of Art clubs and organizations, as well as subscribe to our division list serves and engage with us on facebook!
Like us on Facebook at www.facebook.com/UoASoA!
► Contact Faculty in your area of interest or our Academic Advisors to find out more on how to get involved.
Scholarships & Financial Aid
The School of Art awards over $75,000 in scholarships each year. Scholarships are awarded during the spring semester for the following academic year.
All students are encouraged to apply for:
- School of Art Endowed Scholarships
- College of Fine Arts Medici Scholarship
Additionally, graduate students are eligible to apply for the following grants and scholarships:
- Graduate College Professional Travel Grants
- UA Arts, Humanities, and Social Sciences (AHSS) 1885 Fellowships
Current students must have at least a 3.0 GPA. GPA will be verified prior to review.
Submission of a FAFSA is required for all students who are recommended for scholarships (see details below).
How to Apply
The online scholarship portal is available for approximately one month during the spring semester. List Serve announcements will be posted to notify students when the application is available. Once you have registered, you may return to the scholarship portal as many times as needed to complete your application.
Recommendations for awards are based on the following factors:
- Quality of artwork or writing sample submitted
- 3.0 cumulative and major GPA
School of Art recommendations are forwarded to the University Scholarship Office. You will be notified by email (UA account only) that the recommendation has been made. Scholarships will not be awarded until the scholarship office verifies eligibility.
Student Employment
The School of Art offers student employment as studio technicians and graders. These appointments are competitive and are assigned by faculty. The University of Arizona also posts job opportunities on Wildcat Joblink.
Federal Financial Aid
Free Application for Federal Student Aid (FAFSA) forms are available on-line or through the Office of Student Financial Aid website. Submission of a FAFSA is strongly encouraged for everyone and is required for all students who are recommended for scholarships. These applications are used for the following federal student financial aid programs: Pell Grants, Stafford Loans, SEOG, Perkins Loans and College Work-Study. For more information on student financial aid, please contact the Office of Student Financial Aid.
Additional Funding Opportunities
Financial Aid for Graduate Students
Financial Aid for Graduate Students
Graduate student financial aid awards are competitive and assigned during both fall and spring semesters.
Graduate Tuition Scholarships
Graduate Tuition Scholarships are allocated annually to divisions within the School of Art. The School awards approximately $200,000 in Graduate Tuition Waivers each year. Faculty recommend graduate students for the tuition waivers; all accepted incoming graduate students and continuing graduate students are automatically considered. Graduate Tuition Scholarships may cover any portion of base graduate student tuition, excluding miscellaneous fees
To be eligible for tuition scholarships, graduate students must:
- Be currently admitted and enrolled in a graduate degree-granting program at The University of Arizona for three or more graduate units
- Have a cumulative graduate GPA of 3.00 or higher.
The School of Art has various fellowships that are awarded according to division, including the Museum Studies Fellowship and the Photography Excellence Fellowship. These fellowships are competitive, meritorious, and awarded by the faculty. In addition, there are rotating fellowships that are awarded to three divisions each year. Fellowships are cash awards that are disbursed through the Bursar's Office.
Graduate Assistantships
Graduate assistantships are competitive and assigned by faculty. The responsibilities of a graduate assistant could include teaching, providing technical assistance or grading in an area. Graduate assistants must register for a minimum of 6 units per semester, with a maximum of 16 units. Graduate assistants must also maintain a 3.0 cumulative GPA. The assigned class must meet minimum enrollment requirements. In general, Studio Art and Art History students must also complete a Practicum before they are eligible to teach.


text:
Recently, Solange set off a minor Twitter shitstorm by calling out the writers who big-upped 2012′s avant-R&B renaissance (a renaissance that includes, among others, Solange) as being insufficiently steeped in and reverent of R&B's recent history. As a litmus test of sorts, she mentioned Brandy deep cuts, but her affections, it turns out, also run toward brilliant '90s one-hit wonders. While playing a show last night in London, she invited the xx's Oliver Sim, coolly sheepish as ever, to the stage to help her cover "Make It Hot," an absolutely wonderful minor 1998 hit, a Timbaland/Missy Elliott production for Nicole Wray. Watch fan footage of them doing the song justice below.
(via Pitchfork)
Solange's True is out now on Terrible. Shout out to that London crowd for knowing all the words to "Make It Hot"; that's awesome.
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text:
East of Eden
From Wikipedia, the free encyclopedia
The Bible mentions East of Eden as the location of the Land of Nod, where Cain was exiled.
East of Eden may also refer to:
- "East of Eden", a 1939 novel also known by the title "Comrade Nachman" by Yiddish writer Israel Joshua Singer.
- East of Eden (novel), a 1952 novel by Nobel Prize winner John Steinbeck
- East of Eden (film), a 1955 motion picture based on the Steinbeck book
- East of Eden (TV miniseries), a 1981 television miniseries starring Timothy Bottoms
- East of Eden (1988 film), a 1988 Syrian documentary film by Omar Amiralay
- East of Eden (TV series), a 2008 South Korean television series
- East of Eden (band), a progressive rock band from the UK
- East of Eden (album), a 2009 album by Taken by Trees
- Far East of Eden, a Japanese roleplaying videogame series
- Eden of the East, a 2009 anime television series
- "East of Eden", a song on the album Steeltown by Big Country, a rock band from Scotland
- "East of Eden", a song on the album Fuck Forever by the British band Babyshambles
- "East of Eden", a song on the album Century Spring by American pop-folk singer-songwriter Mason Jennings
- "East of Eden", a song on the album Lone Justice by American roots rock band Lone Justice
- "East of Eden", a song on the album Dead Can Dance by neoclassical dark wave band Dead Can Dance
See also[edit]
- West of Eden, a 1984 science fiction novel by American writer Harry Harrison
|This disambiguation page lists articles associated with the title East of Eden.
If an internal link led you here, you may wish to change the link to point directly to the intended article.


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Mathew Fox DUI, Oprah Loses Shirt
Today's Hollywood Hits features a movie that has made tons of money in one weekend and a media superstar who potentially has lost tons of money with her network. Oh, we also will let you […]
Katy Perry Reveals Surprise On Oprah
Singer [lastfm link_type="artist_info"]Katy Perry[/lastfm] recently announced that she would be revealing a surprise upon appearing on this Monday's episode of Oprah.
Hollywood Hits: Snooki Has A Book, Beyonce Has $350,000 in Purses
Today's Hollywood Hits is loaded. Not only does Beyonce have a ton of purses, but Oprah "built it" and people came, and Katy Perry wants to be Cat Lady.
Hollywood Hits: Hugh Jackman Crash Lands On Oprah
Here are your Hollywood Hits…there's craziness on the set of Oprah's Australian show…there's news about Scarlett and Ryan's split…Charlie Sheen's a free man.
Hollywood Hits: Aretha Franklin Needs Prayers
Aretha Franklin has had some life changing events take place. Click 'more' to hear today's Hoillywood Hits.
Hollywood Hits: Harry Potter's Out, Cher's Dating, And Oprah's Giving
There's a lot going on in the Hollywood Hits. Oprah is having a GREAT day. Harry Potter is out this weekend. Beyonce is banned. See. Told ya. Click 'more.'
Oprah Gets Them Going Nuts…And To Australia
Well, the first day of the last season started off with some craziness on Oprah. John Travolta and Paul Simon help Oprah get the new fall season off to a great start. Click 'more' for […]
Before Oprah loved Stedman, there was another, who?
Diane digs up the dirt……click MORE to see who it is:
Cool..Celebrity Easter Eggs from the 101.9 Lite FM Morning Show!
Alice in Wonderland in EGG! To see Hannah Montana and The Beatles click more:
Rosie is back!
Remember when Oprah Winfrey announced she was pulling her talk show in 2011?


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On Friday, the Cynical Musician posted an article titled Demystifying Creativity, in which he asks the question, "What is creativity?" It's an interesting post, worth a read, and it got me thinking.
I find myself in agreement with much of what Faza says, but I wanted to look at the question through the lens of copyright law. To do so, I'll eventually get to The Hunger Games. But first, I'd like to look at the concept of "genius."
Genius and Generation
Copyright, at its dawn in the US, was often spoken of in terms of "genius." In March 1783, the US Continental Congress appointed a committee to "consider the most proper means of cherishing genius and useful arts through the United States by securing to authors or publishers of new books their property in such works."1 In May, the committee issued its report, stating that it was "persuaded that nothing is more properly a man's own than the fruit of his study, and that the protection and security of literary property would greatly tend to encourage genius."2 The pre-constitutional state copyright statutes of Connecticut, Georgia, and New Hampshire were titled Acts "for the encouragement of literature and genius." The acts of New York and North Carolina both adopted the Continental Congress's language that securing the rights of literary property would encourage genius and "persons of learning and genius". During the ratification of the Constitution, future Supreme Court Justice James Iredell also referred to the Copyright Clause as an encouragement to genius.3
Nowadays, we often think of a "genius" as someone with exceptional intelligence. But at the time, the term was broader: "a man endowed with superior faculties; mental power or faculties; disposition of nature by which any one is qualified for some peculiar employment."4 The word comes from the Latin genius, an aspect of ancient Roman religion. It was used both to describe the "guardian deity or spirit which watches over each person from birth"5 and the "rational soul of every one."6
The latin term itself is derived from the Proto-Indo-European root "*gen-", meaning "to produce" or "to beget", and it shares a common heritage with words like "generate". I mention this because it is interesting how the purpose of copyright, to encourage genius, and the scope of copyright, protection over what a creator generates, share an etymological relationship.
Genius and Copyright
Interesting because the exclusive rights only extend to what an author personally creates. "The sine qua non of copyright is originality."7
To qualify for copyright protection, a work must be original to the author. Original, as the term is used in copyright, means only that the work was independently created by the author (as opposed to copied from other works), and that it possesses at least some minimal degree of creativity. …
Originality is a constitutional requirement. The source of Congress' power to enact copyright laws is Article I, § 8, cl. 8, of the Constitution, which authorizes Congress to "secur[e] for limited Times to Authors . . . the exclusive Right to their respective Writings." In two decisions from the late 19th century—The Trade-Mark Cases, 100 U. S. 82 (1879); and Burrow-Giles Lithographic Co. v. Sarony, 111 U. S. 53 (1884)—this Court defined the crucial terms "authors" and "writings." In so doing, the Court made it unmistakably clear that these terms presuppose a degree of originality.
In The Trade-Mark Cases, the Court addressed the constitutional scope of "writings." For a particular work to be classified "under the head of writings of authors," the Court determined, "originality is required." The Court explained that originality requires independent creation plus a modicum of creativity: "[W]hile the word writings may be liberally construed, as it has been, to include original designs for engraving, prints, &c., it is only such as are original, and are founded in the creative powers of the mind. The writings which are to be protected are the fruits of intellectual labor, embodied in the form of books, prints, engravings, and the like."
In Burrow-Giles, the Court distilled the same requirement from the Constitution's use of the word "authors." The Court defined "author," in a constitutional sense, to mean "he to whom anything owes its origin; originator; maker."8
The idea/expression distinction is derived from this same reasoning. Facts and ideas do not owe their origin to any individual.
The author of a book comes to the great ocean of human thought which belongs to all; he dips up a portion of the brine, evaporates it, causes it to crystallize, purifies the crystals from unpleasant ingredients, and presents it in a new form, a form by which it is made his own. He enters the great forest of ideas, which is common ground, hews down trees, shapes them into articles of furniture, or builds a house with them, and he who takes from him that furniture is a thief, and he who breaks into that house is a burglar. The author clothes ideas in words of his own selection, forms the words into sentences of his own construction, gives the ideas his own arrangement, combines and illustrates them in his own manner, and in this state they are his own, made so by his labor, skill and invention, and they belong as properly to him as the product of salt-works on the edge of the sea belongs to the manufacturer.9
"No Man Writes Exclusively"
Above, we see recognition of the fact that creators always build on the works of the past. No creator creates in isolation, but instead "stands on the shoulders of giants." And progress in the arts and sciences is made by encouraging genius: the originality that a creator generates.
Justice Story said as much in 1845:
In truth, in literature, in science and in art, there are, and can be, few, if any, things, which, in an abstract sense, are strictly new and original throughout. Every book in literature, science and art, borrows, and must necessarily borrow, and use much which was well known and used before. No man creates a new language for himself, at least if he be a wise man, in writing a book. He contents himself with the use of language already known and used and understood by others. No man writes exclusively from his own thoughts, unaided and uninstructed by the thoughts of others. The thoughts of every man are, more or less, a combination of what other men have thought and expressed, although they may be modified, exalted, or improved by his own genius or reflection. If no book could be the subject of copy-right which was not new and original in the elements of which it is composed, there could be no ground for any copy-right in modern times, and we should be obliged to ascend very high, even in antiquity, to find a work entitled to such eminence. Virgil borrowed much from Homer; Bacon drew from earlier as well as contemporary minds; Coke exhausted all the known learning of his profession; and even Shakespeare and Milton, so justly and proudly our boast as the brightest originals would be found to have gathered much from the abundant stores of current knowledge and classical studies in their days. [Emphasis added.]10
Even classical fair use can be said to embrace these ideas. "From the infancy of copyright protection, some opportunity for fair use of copyrighted materials has been thought necessary to fulfill copyright's very purpose, "[t]o promote the Progress of Science and useful Arts."11
[T]he author's consent to a reasonable use of his copyrighted works ha[d] always been implied by the courts as a necessary incident of the constitutional policy of promoting the progress of science and the useful arts, since a prohibition of such use would inhibit subsequent writers from attempting to improve upon prior works and thus . . . frustrate the very ends sought to be attained.12
It's a misnomer, then, to claim that the idea that "everything is a remix" somehow negates the basis for copyright.
But there are those who do indeed claim that the idea that creativity is built on the past is foreign to a legal framework that protects specific expression. There are those who say copyright "stifles" creativity, or those who say, "our system of law doesn't acknowledge the derivative nature of creativity", or academics who quote Michel Foulcault and speak of the notion of "Romantic authorship" — even though the cumulative nature of creativity is baked into copyright law.
The Genius of The Hunger Games
With that, let's turn to The Hunger Games as an example.
Suzanne Collins' hit trilogy (and now feature film) tells the tale of a dystopian future where children are forced to battle to the death. Since it was published, a few have compared it to the 2000 Japanese film Battle Royale, about a dystopian future where children are forced to battle to the death. Some have even asked if the former was a rip-off of the latter. Collins, however, has said in interviews that she had never watched Battle Royale before writing her books,13 and aside from the central conceit, both stories differ substantially.
In other interviews, though, Collins admits that she had drawn inspiration from another, existing story: the ancient Greek myth of Theseus and the Minotaur. Says Collins, "In her own way, Katniss [the heroine of The Hunger Games] is a futuristic Theseus." And in its own way, Hunger Games is a "remix" of the ancient Greek myth.
But would it be copyright infringement? Obviously, the myth of Theseus was written thousands of years before copyright even existed, but let's say it wasn't. And obviously, as with most myths, there is no single, definative version of the story, but let's say there was (for example, Plutarch's version, 75 A.D., trans. John Dryden). Even then, there wouldn't be any verbatim copying between the two. And you'd be hard-pressed to make the case for nonliteral substantial similarity between the stories.14 Though the two may share some plot elements and stock characters, the differences far surpass any similarities.
The Hunger Games, in other words, though it builds on existing works, is decidedly original in the copyright sense; it is a product of Collins' genius in the classical sense.
- 24 Journals of the Continental Congress 180. [↩]
- 24 Journals of the Continental Congress 326. [↩]
- Answers to Mr. Mason's Objections to the New Constitution Recommended by the Late Convention at Philadelphia, in Pamphlets on the Constitution of the United States, pg. 361 (1788). [↩]
- Thomas Sheridan, A General Dictionary of the English Language (1780). [↩]
- "Genius", Online Etymology Dictionary. [↩]
- St. Augustine, City of God, VII.13 (trans. Marcus Dods, 1887). [↩]
- Feist Publications, Inc. v. Rural Telephone Service Co., 499 US 340, 345 (1991). [↩]
- Id. at 345-46. [↩]
- Speech of William Cullen Bryant, International Copyright: Meeting of Authors and Publishers, at the rooms of the New York Historical Society, pg. 14 (1868). [↩]
- Emerson v. Davies, 8 F.Cas. 615 (D. Mass. 1845). [↩]
- Campbell v. Acuff-Rose Music, 510 US 569, 575 (1994). [↩]
- Harper & Row, Publishers v. Nation Enterprises, 471 US 539, 549 (1985), quoting H. Ball, Law of Copyright and Literary Property 260 (1944). [↩]
- In order to copy a work, one must have seen the work. In a copyright infringement case, access to the work is one of the elements the plaintiff must prove, see, e.g., Ferguson v. National Broadcasting, 584 F.2d 111 (5th Cir. 1978). [↩]
- As the Second Circuit has stated, substantial similarity "requires that the copying [be] quantitatively and qualitatively sufficient to support the legal conclusion that infringement (actionable copying) has occurred. The qualitative component concerns the copying of expression, rather than ideas [, facts, works in the public domain, or any other non-protectable elements]…. The quantitative component generally concerns the amount of the copyrighted work that is copied." Castle Rock Entertainment v. Carol Publishing Group, 150 F.3d 132, 138 (1998), quoting Ringgold v. Black Entertainment Television, Inc., 126 F.3d 70, 75 (2d Cir. 1997). [↩]


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Book your room at SpringHill Suites by Marriott Wichita East at Plazzio
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Hotel with an indoor pool, in Wichita (East Wichita)
This hotel is located in Wichita (East Wichita), close to Towne East Square. Area attractions also include Wichita State University and Kansas Aviation Museum.
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Recreational amenities at SpringHill Suites by Marriott Wichita East at Plazzio include an indoor pool and a 24-hour fitness center. Other amenities include complimentary high-speed (wired) Internet access and laundry facilities.
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SpringHill Suites by Marriott Wichita East at Plazzio features an indoor pool and a 24-hour fitness center. Public areas are equipped with complimentary wired high-speed Internet access. Event facilities measuring 370 square feet (33 square meters) include a meeting room. This Wichita hotel also offers coffee/tea in the lobby, complimentary newspapers in the lobby, and laundry facilities. Complimentary self parking is available on site.
SpringHill Suites by Marriott Wichita East at Plazzio is a smoke-free property.
- 24-hour front desk
- Total number of rooms - 102
- Number of floors - 4
- Coffee/tea in lobby
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- One meeting room
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- Conference space size (meters) - 33
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Available in all rooms: Free Wi-Fi , Free wired high-speed Internet
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Recreational amenities at the hotel include an indoor pool and a 24-hour fitness center.
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
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Sony online Entertainment teases new concept art for the 18th expansion to the long-running MMORPG, EverQuest.
EverQuest (EQ) has undergone a number of changes since its 1999 launch, including the revamp of the city of Freeport. I had so many friends at the time that absolutely despised this change and even at times just jokingly wishing that the whole city would burn down. Whether that hatred came from the changes to the city or the fact that many of them were Paladins I'll leave to your own imagination, but someone at SOE must have overheard that conversation and went to work on it. In a new preview screenshot for an upcoming from the Wizard Epic Ornamentation Quest, we get a look at what EQ designer Chris "Dzarn" Black has in mind for players.
While the quest is still months from completion, we do get to see that one thing some of my old friends used to ask for - Freeport burning. Check it out after the break.
Source: EverQuest website
Everquest has just released a new zone preview for its upcoming expansion, Underfoot. The latest zone preview covers the Pellucid Grotto and is home to the race known as the Crystalkin. The...
Over at Flickr, a Tabula Rasa players has posted up sixty-odd scre
A new Screenshot of the Week is posted on the main site for Richard Garriott's Tabula Rasa. Ever wonder how all that AFS Machinery stays operational?
There's always something new to see in Richard Garriott's Tabula Rasa. Long time players will remember the old Boot Camp tutorial, with it's open air control point scenario.
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If you have a hard time fathoming what it must be like to drive in a 24-hour endurance race, you're going to want to watch the video after the break. You'll see an Audi R18 e-tron quattro – the fastest cars on the race track at Le Mans, by a smidge, over the similarly hybrid-powered Toyota TS 030 machines – lap the famed French track after the sun has gone down.
Keep in mind that this video is merely three minutes long, and yet the unflinching dedication shown by driver Tom Kristensen is clearly on display. As an added bonus, we've also included a night lap of the circuit in a Chevrolet Corvette C6 ZR1. Pay close attention about 20 seconds into the second video and you'll see just how
Check out both videos by scrolling down below. Are you as excited for Le Mans this weekend as we are?


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Document Type Doctoral Thesis Author Rutkowska, Daria Anna firstname.lastname@example.com URN etd-09042012-094805 Document Title Characterization of mayor capsid protein VP7 of African horsesickness virus as an antigen display system Degree PhD Department Genetics Supervisor
Advisor Name Title Prof H Huismans Supervisor Keywords
- African horsesickness virus
- antigenic peptides
- VP7 trimers
Date 2012-09-07 Availability restricted AbstractDue to the poor stability and immunogenicity of small antigenic peptides, it is necessary that they be presented to the immune system in conjugation with larger carrier proteins or on the surface of chimeric viral or virus-like particles in order to elicit an immune response. When the major capsid protein VP7 of African horsesickness virus (AHSV) is expressed in Spodoptera frugiperda (Sf9) insect cells by means of a recombinant baculovirus strategy, the VP7 trimers aggregate and are assembled into unique, distinctive crystal-like particles. Immunization of mice with AHSV-9 VP7 crystal-like particles has been found to protect 80-100% of the animals against virulent, heterologous AHSV-7 challenge (Wade-Evans et al., 1997). These results suggested the possibility of using such particles for the immune display of foreign antigenic peptides. Natural hydrophilic loops within the AHSV-9 VP7 proteins' top domain were targeted for the display of foreign peptides by inserting multiple cloning sites into three different positions within the gene encoding the VP7 protein. In this study we explored the feasibility of using the AHSV major capsid protein VP7 as an antigen display system by investigating how peptide insertions into the VP7 top domain affect VP7 particle formation, solubility, trimer formation and immune display.
To generate the VP7 vectors with multiple cloning sites, small 6 amino acid peptides were inserted downstream of amino acids 144, 177 and 200 within the VP7 top domain in previous studies. These VP7 vectors were subsequently used for the insertion of a range of peptides that varied in size from 11 to 150 amino acids. These peptides were derived from outer capsid protein VP1 of foot-and-mouth-disease virus (FMDV), the major neutralization-specific antigen of AHSV (VP2) and well as the envelope protein gp41 of HIV-1. The largest sequence to be inserted into the VP7 top domain was the 239 amino acid enhanced green fluorescent protein (eGFP). To address the question of the effect of foreign peptide insertion on AHSV-9 VP7 particle formation and protein solubility, the expressed chimeric VP7 proteins were analyzed by sucrose density gradient sedimentation analysis. The effect of these peptide insertions on the ability of the AHSV-9 VP7 protein to form trimers was investigated by either a low density sucrose sedimentation strategy or a modified SDS-polyacrylamide gel electrophoresis strategy. The ability of an inserted peptide to elicit an immune response, when presented on the surface of the VP7 trimer, was determined by inoculating the chimeric VP7 fusion protein into guinea pigs and analyzing the humeral immune response elicited by means of insert-specific ELISA and neutralization assays. Since these insertions affected VP7 protein solubility to a significant degree, an important question that was addressed was whether the chimeric VP7 particles or the soluble chimeric VP7 trimers presented the best display strategy for eliciting a strong insert-specific humeral immune response. The effect of various solubilization strategies, such as protein sample dilution and treatments with the solubilization agents L-arginine and sarkosyl, on chimeric VP7 protein solubility were also investigated as well as the effect of these treatments on the immunogenicity of the inserted peptide.
The insertion of different 6 amino acid peptides downstream of amino acids 144, 177 and 200 in the AHSV-9 VP7 top domain during vector construction affected VP7 protein solubility. The inserted peptide and insertion site were both found to play a role. The insertion of a 6 amino acid peptide downstream of VP7 amino acid 144 converted the hydrophobic VP7 protein to a largely soluble protein whereas the insertion of such a peptide downstream of amino acid 200 had no apparent effect on solubility. Although the ability of the VP7 protein to form trimers was not affected, the stability of these trimers under strong denaturing conditions, such as in the presence of SDS at room temperature, was dependent on the site of insertion. Trimers with peptides inserted downstream of VP7 amino acid 177 were the most stable whereas the insertion of peptides downstream of amino acid 200 resulted in the least stable trimer molecules. The insertion of larger foreign peptides into the VP7 vector proteins also influenced protein solubility. Insertion of a 36 amino acid peptide from the FMDV VP1 protein (aa129-164) into site 177 yielded the largely soluble chimeric VP7-177(P1)-FMDVepi protein. A marked reduction in solubility was observed when the same peptide was inserted into a different site. VP7 solubility and particle formation was also strongly affected by the length and amino acid composition of the inserted peptide. This demonstrated that both the VP7 top domain insertion site and the nature of the inserted peptide influence the overall equilibrium between the soluble VP7 chimeric trimers and VP7 particles. It is, as yet, not possible to predict chimeric VP7 protein solubility and the solubility of each construct needs to be individually assessed. The insertion of these larger peptides did not affect the ability of the VP7 fusion proteins to form trimers but trimer stability was again affected as described in the case of the VP7 vector proteins. The only exception was the VP7-200(P1)-eGFP protein, the majority of which occurred in a monomeric rather than in a trimeric form. As both the VP7-144(P2)-eGFP and VP7-177(P1)-eGFP fusion proteins formed trimers, this indicated that the AHSV VP7 trimer is a stable molecule capable of accommodating even large insertions within its top domain without losing its ability to form trimers.
A comparison of the insert-specific humeral immune response elicited against the FMDV VP1 peptide (aa129-164) presented either on VP7-FMDVepi particles or soluble VP7-FMDVepi trimers indicated that, whereas the particles elicited a weak FMDV-specific, neutralizing humeral immune response in guinea pigs, the soluble trimers induced a much stronger immune response. This result focused much of our investigation away from the use of insoluble AHSV VP7 particles as an antigen display system to the use of soluble AHSV VP7 trimer molecules. The large quantity (6.5mg) of soluble VP7-177(P1)-FMDVepi trimers harvested from 1x108 baculovirus-infected insect cells made this a feasible strategy. In order to increase the soluble component of less soluble VP7 proteins, various strategies were investigated. One such strategy involved the substitution of an uncharged leucine residue in position 345 within the bottom domain of the AHSV VP7 protein, with a positively charged arginine residue, the bluetongue virus (BTV) VP7 equivalent. Not only did this mutation significantly increase the solubility of the modified VP7 proteins, their ability to form stable trimers was not affected. This modification may thus be incorporated into future vector proteins to increase VP7 protein solubility. Treatments aimed at reversing VP7 trimer-trimer aggregation were also investigated as a means of increasing VP7 protein solubility. Dilution of the protein sample and/or treatment with the amino acid L-arginine (Tsumoto et al., 2004) was found to reverse the loose aggregation of correctly folded VP7 fusion trimer molecules while treatment with the ionic detergent Na+N-Lauroylsarcosine (Sarkosyl) solubilized both correctly folded and incorrectly folded trimer aggregates. Inoculation of the chimeric VP7 antigen in the presence of L-arginine or sarkosyl elicited a more consistent, strong immune response than untreated, soluble VP7-177(P1)-FMDVepi trimers. This indicated the importance of presenting soluble, non-aggregated antigen to the humeral immune system and that these treatments did not negatively affect antigen immunogenicity. Although both the L-arginine and sarkosyl treatments elicited equivalent humeral immune responses against the VP7-177(P1)-FMDVepi trimers, L-arginine is not required to be removed from the immunogen solution prior to inoculation and, as this amino acid is capable of solubilizing aggregates of correctly folded trimers, it is currently the preferred solubilization protocol.
The eGFP protein folds equally well into its functioning, fluorescing conformation when inserted into any of the three top domain sites. Thus peptides inserted into these sites would most likely have the opportunity to fold correctly into their native conformations. It was, however, observed that the chimeric VP7-144-eGFP fusion protein, although apparently correctly folded and fluorescing, had a tendency to form loosely assembled aggregates that were readily solubilized by simple dilution or treatment with L-arginine. This was in contrast to the VP7 fusions with eGFP inserted at sites 177 and 200, which both had smaller soluble fluorescing fractions, and were not significantly affected by these solubilization treatments. The tendency of correctly folded VP7-144-eGFP fusion trimers to assemble into loosely aggregated particles is also observed with other inserted peptides, such as the AHSV VP2 110 amino acid peptide, and has formed the basis of a low speed centrifugation strategy designed to harvest large quantities of correctly folded, soluble VP7 fusion trimers.
The VP7-HIV fusion proteins were found to be largely insoluble when expressed in insect cells via a recombinant baculovirus strategy and were subsequently explored with respect to their potential to detect HIV antibodies in HIV patient sera. The different binding profiles observed on immune blots against a range of VP7-HIVgp41 fusion proteins could be used to characterize HIV-1 antisera as either subtype B or C but this strategy needs to be further explored. In one of the sera an antibody response against the small ELDKWA epitope on the gp41 protein of HIV-1 subtype B was detected.
The results of this study indicate that foreign peptides may be displayed by AHSV major capsid protein VP7 either on the surface of soluble VP7 trimers as a vaccine display system or by insoluble VP7 particles, in a denatured form on immunoblots, as an antiserum characterization strategy. As it is possible to produce more soluble AHSV VP7 trimers by modifying the VP7 protein, it may also be possible to increase the yield of core-like particles (CLPs) when this protein is co-expressed with AHSV VP3 in recombinant baculovirus-infected cells. Although the size of the presented peptide may be limited by a CLP strategy, these larger particles may induce cellular immunity. Although not yet investigated, it is likely that the soluble VP7 trimers may only elicit a humeral and not a cellular immune response.
© 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
Please cite as follows:
Rutkowska, DA 2012, Characterization of major capsid protein VP7 of African horsesickness virus as an antigen display system, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-09042012-094805 / >
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Trent Beach
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"This is from Paul Prudhomme's Louisiana Kitchen (Copyright 1984). I love this rice and have made it many times over the years. I generally make it just as written except I use a medium casserole with lid instead of messing with the loaf pans and foil. Thanks for posting Suz..."
"Made these to use with grilled brats just like they do in Wisconsin and they turned out great. We didn't have any molasses so I left it out. They were perfectly done after about 17 minutes. I started with 3 1/2 cups of flour (as specified in a different recipe - you can always add more if needed) and added about another cup while running with ..."
"This turned out really good. It was also really easy to do. I didn't bother with covering the crust with plastic wrap - Just flour the roller a bit. The weight measurements as given worked just fine. I left it in the fridge for three days before making - I'm going to try freezing it next."
"Really good and spicy with hot sausage..."
"Try adding the bacon grease from the pan to the mayo...It really adds to the bacon flavor (and fat content!)"
"These were good, but I think they would be better with a bit of white flour. The texture suffers a bit with only whole wheat..."
"Rolls were super! Dough was really easy to work with. Best rolls I ever made..."
"Easy as could be, and delicious. Made two batches, one with chives and green olives and one with less rice, more egg and bacon. Both were awesome. Great way to use up that leftover rice..."