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Stacy Lewis' First Round Interview at the 2014 Sime Darby LPGA Malaysia
Topics: Interviews, Sime Darby LPGA Malaysia, Lewis, Stacy
Na Yeon Choi had the 100th win by a Korean on the LPGA Tour and she talks about what that means to her. Choi also gives a look inside her bag and talks about her goal to be No. 1.


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Find commute times to your work, school, gym and more.
- This property is no longer listed on Rent.com. For this reason please note that any pricing or description information may not be up-to-date.
Bridle Ridge Walk Subdivsion
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Property Features
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Property Details
Well maintained home in quiet subdivsion outside the city. Easy access to I-20. Great schools.
~3 BR/2 BA Brick front home with upgrades throughout
~Great location (Close to Stonecrest Mall and two minutes from I-20)
~Ranch open floor plan
~Gourmet kitchen with upgraded counters and cabinetry.
~Hardwood Floors in living room, dinning room
~New carpet tiles
~Spacious Master Bedroom
~Spacious Master Bath with large Closet
~Swimming Pool and Clubhouse
~Well maintained and gated community.
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No Pets. Call for service animal policy.
One year
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no one told her there was a glass door on the stage
(Lady Gaga)
Check out more sweet decanters over at Inthralld!
OMG Desu Desu so Cool!
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President John Dramani Mahama yesterday commissioned a 273 million dollar water project with a call on Ghanaians to pay for utilities to enable the companies to serve them better. He said government…
President John Dramani Mahama yesterday said government would not spare the rod if any official engaged in or abet any corrupt practices. He said government would take all kinds of measures, includi…
President John Dramani Mahama has announced a 450-million-dollar package for the rehabilitation of roads in cocoa-growing areas in the country. He said the amount to be released in three installmen…
Jane Naana Opoku-Agyemang, the Education Minister, has asked teachers to be more innovative in the teaching of science particularly at the basic school level to make it interesting to pupils.
She said the low number of students found in the science faculties in many schools was due to poor management of teaching and learning of the subject at the basic level of education.
She said this when she opened a four-week workshop for 240 selected science teachers and laboratory technicians from across the country in Kumasi.
They were drawn from Senior High Schools (SHS) and the programme was on the theme: "Education in science – Our benefit and the future".
Prof Naana Opoku-Agyemang underlined the government's determination to adequately equip teacher-trainees with the needed skills to efficiently teach science, mathematics and Information Communication Technology (ICT).
She conceded that, there were indeed problems of insufficient qualified science teachers, laboratory equipment and chemical supplies and said this would be addressed.
Mrs Georgina Quarshie, Director, Science Education Unit, Ghana Education Service (GES), said the workshop was part of the Phase Three of the Science Resource Centre (SRC) Programme and that 1,000 teachers from schools, colleges and the universities would benefit.
They would be exposed to the pedagogy of modern science teaching, practical ICT skills, practical laboratory work and organizational skills.
Source: GNA


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Revenge of the Sith (US - DVD R1)
We have early details on the 3rd of the latest Star Wars prequels
Starring: Ewan McGreggor
Released: 1st November 2005
Further Details
Lucasfilm has today officially announced Star Wars: Episode III Revenge of the Sith which stars Ewan McGreggor and Hayden Christensen. The film will arrive in seperate widescreen and full screen editions from the 1st November this year, with each setting you back around $27.95. Both will carry an English Dolby Digital 5.1 EX track. Extras will include an audio commentary, a brand-new full-length documentary produced by Lucasfilm Ltd, two new featurettes on the prophecy of Anakin Skywalker, and the movie's amazing stunts, a 15-part collection of Lucasfilm's groundbreaking web documentary series, and a two-level playable demo of Star Wars Battlefront II. Completing the package will be some deleted scenes and trailers. We've attached the official package artwork below, along with a couple of screen shots from one of the new deleted scenes which chronicles the birth of the Rebel Alliance. Further details will follow.
Revenge of the Sith: Artwork
Revenge of the Sith: Disc One Menus
Revenge of the Sith: Deleted Scene
News by Tom Woodward
Follow our updates
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jobs Townsville
1-15 of 1,483
Insolvency Senior Manager. 139-113k.
Insolvencyjobs - Townsville QLD
Insolvency Senior Manager. 139-113k. Apply Location: Townsville & Northern Qld, QLD Salary: $149,999. 110,000 Category: Insolvency. Senior Manager You are...
6 days, 15 hours ago in Job Diagnosis
Retail Sales People - Showroom Sales Consultant (Full Time)
Nickscali - Townsville QLD
Retail Sales People. Showroom Sales Consultant (Full Time) Townsville, QLD Be integral to the growth of a successful and iconic Australian business...
2 days, 15 hours ago in Job Diagnosis
Graduate Environmental Engineer/Scientist (Townsville)
Aecom - Townsville QLD
Requisition/Vacancy No. 107239BR Position Title Graduate Environmental Engineer/Scientist (Townsville) Job Category Engineering. Environmental Business Line...
6 days, 15 hours ago in searchaustralianjobs.com
Planning & environment lawyer tropical north coastal qld...
Focus Recruitment - Townsville QLD
Look outside the square! A role offering variety, flexibility, autonomy and transparently defined career p+e law prospects. Dominant market share legal
6 days, 15 hours ago in Jobs Employment Board
Graduate Civil Engineer-WIS (Townsville)
Aecom - Townsville QLD
Requisition/Vacancy No. 105687BR Position Title Graduate Civil Engineer-WIS (Townsville) Job Category Engineering. Civil Business Line Water Office Region.
2 weeks, 2 days ago in searchaustralianjobs.com
Productivity co-ordinator
Innisfail Babinda Cane Product - Townsville QLD
PRODUCTIVITY CO-ORDINATOR Innisfail Babinda Cane Productivity Services PO Box 77, Mourilyan QLD 4858 Ph: (07) 4064 3--- abn 89 100 850 --- productivity co
1 week, 6 days ago in Jobs Employment Board
Senior Quantity Surveyor/Estimator
Aecom - Townsville QLD
Requisition/Vacancy No. 106290BR Position Title Senior Quantity Surveyor/Estimator Job Category Cost Management and Consulting Services Business Line Program...
4 weeks ago in searchaustralianjobs.com
Mechanical Engineering (m/f) - Townsville
Townsville QLD
Your tasks: Analyzing theoretical blueprints. Testing prototypes. Carrying out researches. Suggesting procedures to modify products. Assessing technical...
5 days, 15 hours ago in job-idea.de
Enrolled Nurse
Townsville QLD
Job Ad Reference: TV155321 Location: Surgical Ward One, Surgical Service Group, The Townsville Hospital, Townsville Hospital and Health Service. Salary rates...
6 days, 15 hours ago in qld.gov.au
Venue / Staff Manager
Ready Set Staff - Townsville QLD
Our client is a leading restaurant, currently looking for a Venue / Staff Manager, based in Townsville. The ideal applicant will be highly motivated and...
5 days, 14 hours ago in linkme.com.au
Gymnastics Teacher - TOWNSVILLE
Townsville QLD
Full Time Position Must Have Car And Licence Experience Needed Our client is Gymnastic centre, and they are looking for a Gymnastics Teacher to join t.
4 days, 15 hours ago in jobsearch.gov.au
Dermatologist (m/f) - Townsville
Townsville QLD
Your tasks: Instruct interns or residents in diagnosis and treatment of dermatological diseases. Diagnose and treat pigmented lesions such as common acquired...
1 day, 15 hours ago in job-idea.de
Casual Pharmacy Assistant
Townsville & Northern QLD, Townsville QLD
A brilliant opportunity has opened up for a Pharmacy Assistant to join our client, located in North Ward, Townsville, on a casual basis. A brilliant...
3 days, 13 hours ago in jobx.com.au
Team leader - townsville
Townsville QLD
Team leader team leader townsville recovery service applications close 5 january 2015 to apply visit: (please note those applicat.
6 days, 15 hours ago in jobsearch.gov.au
Nuclear Medicine Technologist
Townsville QLD
This company runs a number of Hospital and Private sites across QLD, and are looking for aNuclear Medicine Technologist to join their team. The position is...
6 days, 15 hours ago in gumtree.com.au


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Modern building on gray background, 3D render.© PhotoStocker - See portfolio
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Travel challenge
A week in Puglia
Saturday 29 June 2013
Each week, we invite three companies to offer us their best deal for a specific break. This week: a week in Puglia. Prices are per person, based on two people sharing. Each includes flights from London to Bari on 1 September and seven nights' accommodation.
Citalia - £699
Includes easyJet flights from Gatwick and half-board at the Hotel Sierra Silvana in Fasano. "Puglia boasts some of Italy's most picturesque towns and unspoilt coastline," says Steph Pritchard (0843 770 4443; citalia.com).
easyJet Holidays - £508
Includes easyJet flights from Gatwick and B&B at the Casa Isabella in San Basilio. "This charming hotel is located in a former Ducal Palace so every room is unique," says Mandy Round (0843 104 1000; easyjet.com/ holidays).
Long Travel - £807
Includes Alitalia flights from Heathrow via Milan, car hire and half-board at Masseria Santa Lucia near Ostuni. "This exceptional hotel has a huge swimming pool and delicious food," says Laura Jones (01694 722 193; long-travel.co.uk).
The Independent travel offers: Discover a world of inspiring destinations
Travel blogs
- 1 PlayStation and Xbox hacked by Lizard Squad
- 2 Christmas comes early to Hong Kong, as millions of bank notes spill out onto busy street
- 3 The 'Black Museum': After 150 years, public can visit police's grisly crime museum
- 4 British actor Idris Elba cannot star as James Bond because he is black, says shock jock Rush Limbaugh
- 5 Vagina canoe artist defends herself over 'obscenity' charges
Travelling by train, boat and plane in 2015: what's in store from ferries to winter sports services
TripAdvisor reveals the top places to visit in 2015
Chic new hotels in 2015: From Britain's first football-themed property to a Facebook millionaire's debut desert resort
Yakutsk: Journey to the coldest city on earth
Top 10 travel destinations for 2015: From Haiti and Alaska to Namibia and Iceland
British actor Idris Elba cannot star as James Bond because he is black, says shock jock Rush Limbaugh
Rozanne Duncan: Ukip expels councillor for 'jaw-dropping' comments made in BBC TV interview
Germany anti-Islam protests: 17,000 march on Dresden against 'Islamification of the West'
Ukip member gets into Christmas spirit with Union Flag plea to Santa 'for our country back'
BBC director Danny Cohen: Rising UK antisemitism makes me feel more uncomfortable than ever
Alex Salmond has 'broken his word to the Scottish people' says Scottish Lib Dem leader
Caribbean luxury at its finest
Barbados offers a new slice of holiday heaven at Port Ferdnand and Saint Peter's Bay Luxury Resorts
Rainbow list 2014: The LBGT people who make a real difference
Introducing our annual celebration of the lesbian, bisexual, gay, and transgender people who make a real difference
i100: Who makes your world a better place?
Tweet us the people who deserve some recognition to @thei100 with the hashtag #everydayhero and tell us why.
i100: How to take a wild career break and come back more employable
Fancy a wacky sabbatical?
Build yourself a brighter future
Chartered surveyors will be critical to shaping our new world
Enter the latest Independent competitions
Win anything from gadgets to five-star holidays on our competitions and offers page.
Business videos from commercial thought leaders
Watch the best in the business world give their insights into the world of business.
iJobs Travel
Recruitment Genius: Personal Trainer / PT - OTE £30,000 Uncapped
£25000 - £30000 per annum: Recruitment Genius: The fastest growing fitness cha...
Investigo: Finance Analyst
£240 - £275 per day: Investigo: Support the global business through in-depth a...
Ashdown Group: Data Manager - £Market Rate
Negotiable: Ashdown Group: Data Manager - MySQL, Shell Scripts, Java, VB Scrip...
Ashdown Group: Application Support Analyst - Bedfordshire/Cambs border - £32k
£27000 - £32000 per annum: Ashdown Group: Application Support Analyst - near S...


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Bright Starts Play to Learn Pond Pal
About this item
The Bright Starts Play to Learn Pond Pal is a fun way for infants to learn animal names and language. The drum spins to reveal a different cute picture when your little one presses the frog's head. It also says the name of five different animals in both English and Spanish. This Bright Starts toy is easy for babies to press whether they are crawling or sitting. It also plays delightful sounds when the drum is done spinning and lands on an image. This baby learning toy features a mode for learning and for music to engage your little one.
Bright Starts Play to Learn Pond Pal:
- Press frog's head to spin drum
- Animal name and sounds play when image lands in the opening
- Easy for baby to press
- Introduces baby to 5 different animals in English and Spanish
- Learning and music modes
- Baby learning toy requires 2 AA batteries (included)
|12 - 24 Months , Birth - 12 Months
|Battery Type:
|Carbon Zinc
|Multi Pack Indicator:
|Model No.:
|Shipping Weight (in pounds):
|Product in Inches (L x W x H):
|10.5 x 7.25 x 6.75
|Assembled in Country of Origin:
|Origin of Components:
|Walmart No.:
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Questions will be checked against our question guidelines and posted within five to seven business days.


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26 Laurel Hill Road, BT51 3AU
offers around £84,950
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offers around £475,000
32 Old Coach Way, BT24 7PG
offers around £295,000
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Delaware court rules against Vermillion stockholders group
- Christopher Calnan
- Staff Writer- Austin Business Journal
- Email | Twitter
The Delaware Court of Chancery dismissed with prejudice on Nov. 16 a lawsuit brought against Vermillion Inc. and its board of directors by dissident stockholders.
Following the decision, a proxy filed with the U.S. Securities Exchange Commission by a stockholder group led by Gyorgy Bessenyei and Robert Goggin describes Goggin as "honest and trustworthy."
But the court ruled that Goggin and Bessenyei had illegally falsified documents and that Goggin appeared to be a violation of the Pennsylvania rules of professional conduct applicable to Pennsylvania lawyers, according to a company news release.
Last week, the three-person stockholders group questioned a $400,000 severance package for Vermillion CEO Gail Page — as she remains on the job six months after the company announced her resignation.
Vermillion, a molecular diagnostics company, was founded in 1993 as Abiotic Systems in Freemont, Calif., and moved its headquarters to Austin in 2011. It employs 27 workers.
Last week, Vermillion's legal counsel, Robert Claassen, said the stockholders group call for Page's removal and Chairman James Burns resignation was unprofessional because the chairman is elected by the board.
"That's just rookie land," he said. "That's not how corporate governance goes."
Vermillion posted a $2 million loss on $319,000 in revenue during the third quarter, compared with a $4.7 million loss on $320,000 in revenue during the same three months last year.
Get an early start on the day's top business news. Sign up for the Morning Call news blast email.
Christopher Calnan covers technology, finance and clean energy for the Austin Business Journal. Subscribe to the Energy Inc. newsletter
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He wants to cure you
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New tax starts Wednesday for prepaid wireless
By Peninsula Daily News staff
Print This | Email This
Most Popular this week
Biggest and brightest: Where to see the best holiday lights on the North Olympic Peninsula [with a photo sampler]
Suspected pipe bomb and theft investigation leads to arrest of Port Townsend man already charged in separate burglary
That's when a new state law takes effect that requires retailers of prepaid wireless services to collect the tax that supports enhanced 9-1-1 services, or E9-1-1.
Wireless companies already collect and remit the tax.
Cellphone users under contract pay this tax on each month's bill.
But for prepaid wireless users, the tax was not part of their purchase of phones or minutes. Now, prepaid wireless users will be charged the $1 fee at the cash register to be collected by retailers.
Local governments will receive 70 cents of the tax, and the state will receive 25 cents. The new law will allow retailers to charge 5 cents to cover their cost of collecting the tax until July 1, 2018.
The tax supports the technology that enables local 9-1-1 emergency services to pinpoint the location of cellphone users who call for help.
The E9-1-1 tax is in addition to state and local retail sales taxes.
The tax comes as part of House Bill 1971, a larger telecommunications bill passed by the state Legislature during the 2013 session.
Last modified: December 30. 2013 8:13AM


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We're taking the holiday rush into consideration this year and offering you last minute shoppers 20% off our entire selection of accessories here at Android Central. That means cases, chargers, batteries, Bluetooth and anything else you can find to stuff your stockings with!
With popular devices like the Samsung Galaxy Note 4, HTC One M8, Samsung Galaxy S5 and the Samsung Galaxy Note 3 flying off the shelves this season, you'll want the perfect accessories to accommodate that brand spankin' new Android, right?
After you've done your last minute shopping, simply enter coupon code: LAST14 at checkout to apply your 20% off discount. We offer FREE shipping on all orders over $50 and have even faster delivery options for those who need their Android gear quicker than the norm.
So, get your orders in and save some cash while you can because this sale ends midnight Sunday, December 21st PST.


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8 of 10 people found the following review helpful
Great Emotions Over Mixed Emulsions,
This review is from: Mixed Emulsions: Altered Art Techniques for Photographic Imagery (Paperback)
I received this book as a Christmas present. The minute I came home, I opened it, and because of the clearly written text and most beautiful photographs, I did not put the book down for several hours. The book is well written, clearly describes intricate processes and is most inspriational.
I now keep it on my work table both for reference and for inspriation. I appreciate both the beauty and the sincerity of the book. If you are thinking of buying a book on altered art processes and wanting to be inspired- This is the book for you. Buy it. Enjoy it. Create with it!
Oh and I want to add- it's also a great book to give as a gift!
Review Details
(32 customer reviews)
$24.99 $21.24
Location: San Diego, CA
Top Reviewer Ranking: 10,872,724


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70s -no label- Womens harvest gold polyester short sleeve slightly shorter than mid length a-line mod dress. A rib texture, with shaping darts, shoulder epaulet details, a round neckline and back metal zip closure. The zipper is a little stiff. In stock.
Sale Price:
32Bust 34Hip
Good Condition
Approx Shipping Weight 1.38 lbs.
Item No.
Questions? View our Help Page or contact Jen at firstname.lastname@example.com


text:
Chris Carlson, Associated Press
LOS ANGELES — Blake Griffin scored 20 points, Chris Paul added 18 points and seven assists in his second game back from injury, and the first-place Los Angeles Clippers hung on in the fourth quarter for a 98-91 victory over the Memphis Grizzlies on Thursday night.
Mo Williams scored nine of his 18 points in the fourth quarter, and Griffin added nine rebounds and eight assists as the Clippers maintained a narrow lead throughout the final period. Caron Butler scored 17 points, and Paul also had seven rebounds while playing back-to-back games after missing the previous five with a strained left hamstring.
Rudy Gay scored 24 points for the Grizzlies, who have lost two straight following a seven-game winning streak. Marc Gasol added 18 points and 11 rebounds on Memphis' third stop on a four-game West Coast trip.
- Brad Rock: Bowl drama shows BYU not going...
- Both BYU and Memphis to review bowl game brawl
- Brady Poppinga: Confronting an unlikely opponent
- These 14 Utahns reminded us why we love...
- Sitake, Tuiaki leaving Utah for Oregon State
- BYU's Tyler Haws' recovery from an ankle...
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- Report: Video games experts, BYU head coach...
- Wild one: Memphis edges BYU in... 172
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- Miami Beach Bowl ends in free-for-all... 97
- Dick Harmon: Cougar bowl loss kind of... 59
- Big winner: Utes hit jackpot early and... 55
- Miami Beach Bowl ends in brawl between... 52
- Report: Video games experts, BYU head... 45
- Both BYU and Memphis to review bowl... 45


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Katy Perry was "panting like a dog" while her sister was in labour.
The Dark Horse singer's older sister Angela gave birth to a daughter in her friend's living room back in March.
Self-titled "pop star and child deliverer" Katy was on hand to go through every moment with her sibling.
"I'm an assistant doula, that's what they called me the whole time," the 29-year-old told Jimmy Kimmel Live.
"We had a midwife and there was a tub in the living room, I was holding a leg, and panting like a dog. Like, primal noises coming out."
A doula is a non-medical person who assists during labour. When Katy wasn't cradling her sister's limbs or doing her own heavy breathing, she revealed that she filmed a lot of the process.
"[Angela] decided to go the all-natural way, not a single aspirin," she told the host proudly. "I mean, I don't know how that feels, everything I saw - 'cause I was Scorsese-ing up in there with my video camera, just covering anniversaries and birthdays, y'know what I'm saying - but it was amazing."
Katy, who also has a younger brother David, is close to her siblings and has even included a nostalgic photograph with Angela on the artwork of her new single, Birthday.
In the shot, the two little girls are seen at a party with big bows in their hair. When questioned by Jimmy if she asks her sister's permission before using her image on social media, Katy joked, "Mainly forgiveness!"
While Katy loves being an aunty, she isn't ready for motherhood just yet. She previously revealed to Marie Claire that it was one of the main reasons her marriage to Russell Brand ended in December 2011.
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Comment is free + John Cleese
Terry Jones: We're dusting off the old scripts and giving them an update for our O2 show – who wants to end up like poor Neil Diamond, booed for trying too much new material?
Terry Jones has confirmed that Monty Python are to reunite. Are you looking forward to the prospect of fresh laughs from the wacky comedians, or do you find them about as funny as a dead parrot?
Barbara Ellen: The Diana director is kidding himself if he thinks that we're still mourning the 'Queen of Hearts'
Barbara Ellen: Either Benedict Cumberbatch should stop whingeing or flee to America
The people's panel: So, George Soros is to marry Tamiko Bolton, 42 years his junior. What do you think of large age gaps in couples?
Sarah Ditum: As Robin Williams and John Cleese have recently demonstrated, ostentatious displays of resentment are a grim thing to behold
Andrew Brown: This is a bit of seasonal silliness, timed for the week when everyone is working hardest
Peter Preston: Frustrating, infuriating and costly. But still it is refreshing to see cool heads under empty skies
Editorial: The rules of television comedy were rewritten as John Cleese, Graham Chapman, Michael Palin, Terry Jones, Eric Idle and Terry Gilliam created lunatic characters and sketches, as funny today as they were 40 years ago
Catherine Bennett: The millions that John Cleese's ex-wife is due in alimony highlight the very strange state of feminism nowadays


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Furniture of America Luxer Coffee Table with Sliding Top and Storage CompartmentsItem # HN-ENLB020
- Modern 2-tone table with concealed storage
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Furniture of America Luxer Coffee Table with Sliding Top and Storage Compartments
At first glance, the Furniture of America Luxer Coffee Table with Sliding Top and Storage Compartments is already a beautiful basic. When you move its easy-slide top to the side and reveal a handily divided storage compartment, it becomes downright brilliant. Crafted with durable wood, medium fiber board, and strengthening, warp-resistant veneers, this modern table gets interest from dual finishes - warm wood-tone legs and a fresh white tabletop. It gets innovation from a complete internal organization syst


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Home » Opinion » Columns
Betsy McCaughey: More ways Washington squanders your money
The Environmental Protection Agency is not protecting your tax dollars. According to House Oversight Committee Chairman Darrell Issa, "EPA surpervisors knowingly signed off on time sheets for people they knew. . . did not work, and in fact, never even logged onto their computers." EPA employee John Beale got away with not coming to work for weeks at a time over two decades and charging airline tickets to the agency, all by masquerading as a CIA agent. None of his EPA supervisors has been held accountable for not stopping the costly charade.
Betsy McCaughey is a syndicated columnist and former lieutenant governor of New York.
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LDS Church
President Monson's message of 'Hastening the Work' and its impact on genealogy, temple work
The prophet's message of "Hastening the Work" has affected missionary, genealogy and temple work. As workers on the other side of veil are busy, we see an increase in... Read more »
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text: POPSUGAR Celebrity Charlize Theron Photos of Charlize Theron by Celebrity 8/13/09 0 Reactions 0 Shares Print 0 0 0 0 0 Previous Photo 1 of 4 View on One Page ADVERTISEMENT ( more slides) Next 1 Photos of Charlize Theron Previous Photo 1 of 4 View on One Page ADVERTISEMENT ( more slides) Next 0 0 0 0 0 Read More Charlize Theron Charlize Theron Seth MacFarlane Throws Star-Studded Holiday Bash With Guests Like Charlize Theron, Zac Efron, Robert Pattinson: Details! 5 days ago The Mad Max Trailer Will Blow Your Mind Even If You Never Saw the Original 1 week ago #TBT: All the Times Chris Hemsworth Was a Girl's Best Accessory 3 weeks ago Can't-Miss Celebrity Pics! 4 weeks ago Charlize Theron's Son's Temper Tantrum Leads to a Visit From the Police 11/17 9 Celebrity Names You're Pronouncing Horribly Wrong 11/04 17 Stars Who Could Give You a Language Lesson 11/04 Get the scoop with our daily newsletter Enjoy the latest news on the celebs you love. Young Hollywood Movies A Listers TV Sign up with Link Love Princess Madeleine of Sweden is expecting her second child — People George Clooney woos Downton Abbey's Elizabeth McGovern on set — Us Weekly Azealia Banks reveals why she has such a beef with Iggy — HuffPost Celebrity Tim Burton is not a fan of modern-day superhero movies — Lainey Gossip Betty White gets hilariously wacky with Craig Ferguson — Dlisted Owen Wilson shares an adorable video of his son — ET Find out who Kylie Jenner had a date with — JustJared Wait, Bella Thorne was spotted working where?! — Hollywood Tuna You've never seen Nicki Minaj look this glamorous — Pink Is the New Blog See Charli XCX's crazy Jingle Ball outfit — The Superficial Christina Hendricks moves on to her next big project — popbytes Latest Celebrity & News


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The whirl of hair dyers and friendly chatter fills the hairstylists? work area inside Diversite Salon and Day Spa. Stylists cut hair into new modern 'dos and apply hair-coloring techniques, such as an ombre effect or highlights that change to lowlights during thunderstorms. The spa half of the business relaxes patrons with soothing massages and spray tan sessions.
Michael's Salon and Spa has been locally owned and operated in Centerville for 35 years, resulting in generations of contented curls. Let Michael's latest flock of curl contenters maintain your follicular shrubbery's favorite shape, or ask them to sculpt an entirely new hairbatat to attract expectant butterflies. All of Michael's new designers are cosmetology-school licensed and have completed Michael's six-month to one-year training and internship program, so they're fully prepared to suds sadness out of tresses and style them to suberbity. A neck and scalp massage is included to thank cranium supporters for holding heads up and twisting both ways before crossing streets.
Amy Tobias and Cathy Murray, aestheticians and co-owners of Transforming Techniques Salon and Medical Spa, each bring 17 years of beauty-biz experience to their treatment chairs. They and their team lavish complexions with a wide range of skin services, from antibacterial and oxygen facials to slightly more intensive chemical peels and wrinkle-fighting injections. Stylists tend to men's, women's, and kids' locks, keeping ends fresh and hairdos manageable, and techs groom both natural and artificial nails. Massage therapists knead stress from tense muscles, and depilation experts use wax to whisk unwanted body hair away from faces, legs, and plates of nachos.
New Beginnings Salon & Spa pampers guests from scalp to toe-tip with an indulgent menu of beautification services. After a swift waxing session, guests may retreat to a private room for a restorative facial or therapeutic bodywork. New Beginnings also renovates strands with custom cuts and bold color treatments. Though the stylists specialize in ethnic hair, their expertise spans all types, regardless of color, texture, or astrological sign.
Studio 31 Salons creates classic looks with contemporary touches. Stylists keep scalps and manes classically beautiful with conditioning, restructuring, dry-scalp, and clarifying treatments. Then they shear timelessly chic cuts and add shine with glaze, or they may get more creative with pink ombre or chunky copper highlights that imitate the abstract paintings adorning the salon's robin's-egg blue walls. In addition to hair services, the staff applies permanent eyeliner, lip liner, and eyebrow makeup and offers sessions in a tanning bed to top off revamped visages.
A step inside Beauty Lane Salon & Tanning leads to an airy studio, where natural sunlight streams through windows to illuminate blond-wood floors, leather furnishings, and red accent walls. With this modern space for their inspiration, the stylists here transform their clients' hair through trendy cuts and bold applications of color—be it an all-over hue or the unexpected touch of bright blue feather extensions. Aestheticians, meanwhile, buff and polish nails to a new shine, and technicians oversee tanning sessions in stand-up airbrush booths and a UV tanning bed.


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Dog Earmuffs
Earmuffs: Spoiled Sweet or Spoiled Rotten?
by Pets 17 Reactions 4 Shares Print
With temperatures dropping daily, you can now keep pup's ears toasty with Tartan Fleece Earmuffs, one size fits most. They even feature a neck strap so he can't shake them . . . or rather, so he can keep them on tight. Not sure if this clues anyone in (or changes your mind), but you can pick up a pair for just $6, 88 percent off their suggested retail price of $49.
I'm certain if North managed to get a hold of these earmuffs, he would consider it to be a new plush toy in no time. How would your pet react?
5 years
I think the only dog that would need this would be the Chihuahua.. Haha mine get's SUPER cold easily, and only through her ears. :)
5 years
well,there really cute but my maltie poo yorkie mix mostly yorkie,cassie would just stand still and look at me like uhh? get whatever this is off my head and pronto and i usually always do but if i dont do it quick enough she either just stands there or does it herself and as you can see she is veary extremly spoiled to the core!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
EllaBella 7 years
I think it's cute but I definitely don't think dogs would like wearing that!
chancleta 7 years
1. yuck 2. animals don't like their ears covered
sexyeyes 7 years
I don't think my dog would like them
Angelica 7 years
I can only hope to see a doggie walking down the street with these on! I would flip out and immediately take pic with my cell.
telewyo 7 years
Ridiculous! I'm not sure this is good for a dog either...when my dog was a puppy he had a blood blister on his ear and they fixed it and put a bandage around his head but they told me to check it often because it's really bad to trap too much extra heat in a dog's ears.
Cole 7 years
Somehow I don't think my dog needs ear muffs considering he has long floppy fur covered ears to protect the actual hearing part of his ear. Not only that but I don't think he can feel much in his ears anyway considering he will chew on them when they get in the way of him chewing on my fingers.
Merlin713 7 years
What the heck?! I don't have a dog anymore (we put her to sleep because she was so old and sick), but I am pretty sure that dogs have this natural sort of thing where they stay warm in cold weather......... And the only thing my dog wore when she was alive was a collar. Sometimes that was optional.
Lovely_1 7 years
Taz would hate it too!!!
nikodarling 7 years
This is ridiculous. Neither of my dogs would stand for this. They'd be off and eaten in about 5 seconds.
eastcoastgirl 7 years
If I tried to put these on my dog he would just stand like a statue and not move until I took them off,thatis what he does when he doesn't like something you put on him!!They are cute though!!
Gabriela14815884 7 years
They would stay on for about 0.3 secs before my dog took them off!
GibsonGIrl 7 years
My little princess would not be a happy camper if i put this on her...
mdwilde 7 years
Even with the neckstrap, Vera would just paw at them until they came off. I gave up on making her wear things on her head (like hats to keep warm) a long time ago. She does like wearing hoodies though.
allhailtoyin 7 years
Animals have never shown issues with the winter...
karisaamy 7 years
My dog would kill me if I tried to put these on her
Link Love
- Princess Madeleine of Sweden is expecting her second child — People
- George Clooney woos Downton Abbey's Elizabeth McGovern on set — Us Weekly
- Azealia Banks reveals why she has such a beef with Iggy — HuffPost Celebrity
- Tim Burton is not a fan of modern-day superhero movies — Lainey Gossip
- Betty White gets hilariously wacky with Craig Ferguson — Dlisted
- Owen Wilson shares an adorable video of his son — ET
- Find out who Kylie Jenner had a date with — JustJared
- Wait, Bella Thorne was spotted working where?! — Hollywood Tuna
- You've never seen Nicki Minaj look this glamorous — Pink Is the New Blog
- See Charli XCX's crazy Jingle Ball outfit — The Superficial
- Christina Hendricks moves on to her next big project — popbytes


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Tractor Parts & Accessories
Tuthill/Fill-Rite 115 volt Manual Nozzle Fuel Pump SD602G$319.99
The Fill-Rite Fuel Transfer Pump is designed to provide safer, more efficient transfer of diesel, gasoline, kerosene, mineral spirits, Stoddard solvent (white spirit) and heptane. Thermally protected, explosion-proof UL/cUL listed motor. Two ball bearings. Adjustable suction pipe. 2in. NPT bung adapter and clamp assembly. 4-bolt flange mount. Motor is hardwired to circuit breaker. U.S.A.
- 13 GPM for fast, dependable transfer
- 115 Volt AC with a dependable 1/4 HP motor Motor has sealed bearings, heavy-duty switch and thermal overload protection to ensure years of dependable operation.
- Built-in junction box for easy wiring and installation.
- Cast iron pump housing with iron rotor and carbon vanes is stronger and more durable than aluminum pumps with rotary gears
- Check valve, pressure relief valve and strainer assembly provides consistent flow.
- Automatic bypass valve
- 2in. threaded base for tank openings
- 3/4in. x 12ft. hose with static wire
- UL listed hose is abrasion resistant which protects against corrosion, UV rays, ozone and harsh weather
- Static wire prevents sparking and ensures safety.
- Manual dispensing nozzle.
- Tank Adapter (Bung Adapter): 2in. O.D. x 1.5in. I.D.
- UL/cUL listed.
- Rain proof
- Volts: 115
- Watts: 10
- Amps: 2
- Speed (RPM): 2,600
- Flow (GPM): 13
- Max. Total Head (ft.): 37
- Max. Suction Lift (ft.): 7
- Inlet Port (in.): 1
- Outlet Port (in.): 3/4
- Discharge (ft.): 37
- Duty Cycle: 30 min.
- Self-Priming: Yes
- Bypass Valve: Yes
Fimco 25 Gallon ATV Sprayer with 10' Boom ATV-25-700$349.97
- Fimco model ATV-25-700 ATV sprayer
- This durable high quality sprayer mounts easily to most all terrain vehicles
- Spraying may be accomplished with the 140 in spray coverage boom or handgun with adjustable nozzle
- The heavy duty three section boom folds to the rear for transport or storage
- Fimco Gold Series 12 Volt (Demand) Diaphragm Pump
- 2.1 gpm
Ace Roto Mold Tanks 300 Gallon Horizontal Tank HZ0300-38$320.97
Round Horizontal Tanks are selected when transportable (non DOT approved) containment is required for indoor or outdoor applications.
Tanks require skids and bands to support the round bottom. All tanks are equipped with drain out sumps. Tanks are manufactured from medium-density polyethylene with U.V. inhibitors and designed for containment of liquids of up to 1.7 specific gravity. Tank walls are translucent for level viewing and equipped with gallon indicators.
- Capacity - 300 Gallons
- Weight - 88 Pounds
- Dimension - 38x76
- Lid - 8"
- Fitting - 3/4″ & 1-1/4″
K&M International 06-66 with Cab Series Left Hand Tractor Cab Step Kit 3347$336.97
Convenient step unit makes it easier to get in and out of your tractor. Handrail is available for additional safety. Easy to install with no holes to drill and hardware is included. Step is 14 1/2" wide and 6" deep. It mounts at a 30 degree angle to the tractor and has 9" between each step. Matches original equipment colors. This model fits on left side of IH 06, 26, 56, 66 and Hydro 100 tractors with cabs (excluding Hiniker). Can fit IH 706, 756, 806, 826, 856, 866, 966, 1026, 1066, 1206, 1256, 1456, 1466, 1566 and 1568.
Please Note: Does not fit Hiniker cab or open station tractors. Also will not fit IH 826 German-made models.Learn More
Tuthill/Fill-Rite 12V DC Hi-Flow (Pump Only) FR4204G$305.97
Product Description
Contractor-grade, industry-standard Fill-Rite pump has an explosion-proof motor with permanent magnet. The pump only version, features built-in check valve and strainer, Please see our full line up of meters and accessories to compliment and enhance your pump . Padlockable. UL listed. U.S.A.
Features & Benefits
- UL Approved Motor
- Heavy Duty Cast Iron
- Diesel/Gasoline/Kerosene/E15/BD up to BD20
- 2 year warranty
- 2" Threaded base for tank mounting
- Cat bronze rotor with bronze rotary vanes
- Built in strainer
- 1" x 12' hose with static wire
Sensor-1 GPS Speed Sensor for Raven Monitors 1 Hz GPSMD-R1-BLK$310.97
This Sensor-1 GPS Speed Sensor with a Digital Display is a self contained GPS unit with 1 Update per Second. The Sensor-1 Speed Sensor has an integrated magnetic mount for ease of installation. 3 Pin Conxall Connector for Raven Monitors. 10 Ft Lead.
- Black
- 57 Hz
- 3 Pin Conxall Connector for Raven Monitors
- No Pulse per Second
- 1 Update per Second
- Magnetic Mount
- 10 ft. Lead
Country Way 25 Gallon Boomless 3 GPM ATV Sprayer SSP-A1-025A-RK$329.99Product Features
- 2 Nozzle Boomless Sprayer
- 3 GPM Pump
- 25 Gallon Tank
- Ideal for applying Insecticides & Herbicides
- Round-up® Ready
K&M Mfg IH 56-66 Seat Assembly - Black Vinyl 7165$306.97
Quality replacement seat assembly for your IH tractor. Seat and backrest have steel cores and 4" foam cushions. Armrests have 2" foam cushions. The seat will fit on original mechanical or hydraulic suspensions. Cushions are replaceable separately. Armrests fold up. Available in vinyl or fabric.
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Butte Theater, Cripple CreekChristmas favorites and the Great American Songbook come alive in this stunning Christmas musical! When a group of actors become stranded in the quaint town of Wonderland during Christmas of 1952, ...
Canon City performing arts events
Winter Wonderland
Friday, Dec 19 7:00p
Tossed & Found
Sunday, Jan 11 2:00p
New Year's Eve Bash!
Wednesday, Dec 31 7:00p
Black Box Theatre, Colorado SpringsParty at Black Box with musician Clayton Wyatt, the band No Stone Unturned, our own Improv COLORADO's Improv Jam, and dance 'til midnight as we drop the ball in Times' Square with Guy Lombardo...
Rip Improv
Friday, Feb 27 7:30p
Junie B. in "Jingle Bells, Batman Smells"
Saturday, Dec 27 4:00p
Jim Henson's "Dinosaur Train: Live! Buddy's Big Adventure"
Wednesday, Jan 7 6:30p
Double Bill Willy Wonka/Annie
Friday, Dec 19 7:00p
Colorado Springs Conservatory, Colorado SpringsDouble-bill: CSC Mini Company presents Roald Dahl's Willy Wonka and CSC Pre Mini and Pre Junior Company presents Annie KIDS.
Blue Man Group
Tuesday, Feb 3 7:30p
Tuesday, Mar 17 7:30p
Mamma Mia!
Wednesday, Apr 8 7:30p
Million Dollar Quartet
Tuesday, Jun 2 7:30p
"In the Mood"
Wednesday, Apr 15 2:00p
Saturday, Dec 27 5:30p
The Underground, Colorado Springsunbound A live magazine. A live show featuring artists, musicians, comedians, activists, poets and performers...
Stories from the ROAD
Thursday, Jan 22 7:30p
Dancing with Demons - a fractured fairytale
Thursday, Jan 15 7:30p
Canon City performing arts Related Topics
ballet | cabaret & revue | community theater | literary arts | musicals | opera | poetry | puppetry | street performance | symphony | theater | theaters colorado springs | sex | colorado springs live irish or celtic music | mat theatre colorado springs | haunted house | colorado springs pikes peak center | christmas | arnold hall | family theater | colorado springs world golf trick or treat | actors | mary poppins colorado springs | new years colorado | trunk or treat contest
Hot Tickets More »
Sat 12/20 7:30p
Wed 12/31 4:00p
Fri 2/27 7:30p
Thu 1/22 7:30p
Sun 1/11 2:00p
Thu 1/15 7:30p
Upcoming Holiday Events More »
Winter Wonderland
Fri 12/19 7:00p
"An Enchanting Night under the Stars" Christmas Banquet
Fri 12/19 6:00p
Bolshoi Ballet: The Nutcracker
Sun 12/21 10:55a
Bolshoi Ballet: The Nutcracker
Sun 12/21 10:55a
New Year's Eve
New Years Eve Gala
Wed 12/31 7:00p


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NYSE Euronext
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text:
Jeremy Shane
Jeremy was born in a small mountain village of a strange foreign land called Weystvurginea. Banishment for liberal views saw him spend years wondering the east coast until he decided to bike to California. When he saw how long a trip it was, he drove instead. Now he's living it up in a low humidity climate, sometimes working on his photography and when not, he writes for us covering books (by way of his blog: Reading Realms), gaming, tv, movies, comics, conventions in the SoCal area, and creates a weekly webcomic: A Journey Through Skyrim. If you look for him offline, start in the L.A. area; online start at: www.jeremyshane.info for his profile and all the social networks he's on... or just follow him on twitter, he seems to be on there a lot: @jeremyshane.
Project Eternity from Obsidian Destroys Fundraising GoalsBy xaraan in News with Benefits on October 18, 2012
RPG video game makers Obsidian smashed their own fundraising goals and Kickstarters game-fundraising records with their upcoming 'Project Eternity' game.
Official Call of Duty: Black Op 2 Launch TrailerBy Jeremy Shane in News with Benefits on October 16, 2012
Title says it all, watch it here.
A Journey Through Skyrim #11By Jeremy Shane in Webcomics on October 16, 2012
Issue Eleven of webcomic series: "A Journey Through Skryim" is available!
Next Skyrim DLC: Dragonborn?By xaraan in News with Benefits on October 16, 2012
Rumors and leaks are in the air again as the third Skyrim add-on is discussed.
The Top 5 Biggest Stories from New York Comic ConBy xaraan in Features on October 15, 2012
Plenty of news rolled out of NYCC this weekend, and too much of it trickled out in the weeks before. So what ended up being the big news at the con?
Book Review: Dragonlance Chronicles by Weis & HickmanBy xaraan in Reviews on October 15, 2012
Reading Realms classic reviews takes a look at Dungeons & Dragons based: Dragonlance Chronicles by Margaret Weis and Tracy Hickman.
NYCC: Two New Major Star Wars Novels on the WayBy xaraan in News with Benefits on October 15, 2012
Del Ray publishing managed to squeeze in some book related news amongst the comic book, television, movie and toy madness at NYCC.
NYCC: Jim Lee's Wild C.A.T.S.?By Jeremy Shane in News with Benefits on October 15, 2012
In DC's new52 relaunch, many fans felt the Wildstorm universe got the short end of the stick. Some wondered why they at least did not use Wildcats, well wonder no more.
NYCC: 'Arrow' Panel (Updated with Trailer)By xaraan in News with Benefits on December 31, 1969
Stars Stephen Amell, Katie Cassidy, Willa Holland, producer Marc Guggenheim and others take the stage at NYCC to talk about the future of recently premiered "Arrow"
NYCC: DC Shows off ArtBy xaraan in News with Benefits on October 14, 2012
DC offers fans an advanced look at upcoming covers for some of their titles in the new52.
Page 95 of 127


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/ Muscular Dystrophy
Muscular Dystrophy
Coding Dimension ID:
Coding Dimension path name:
Muscular Dystrophy
Read more
about Generation of clinical grade human iPS cells
Generation of clinical grade human iPS cells
Funding Type:
New Cell Lines
Grant Number:
Zack Jerome
University of California, Los Angeles
ICOC Funds Committed:
$1 382 400
Disease Focus:
Amyotrophic Lateral Sclerosis
Neurological Disorders
Muscular Dystrophy
Neurological Disorders
Stem Cell Use:
iPS Cell
Cell Line Generation:
iPS Cell
Public Abstract:
The therapeutic use of stem cells depends on the availability of pluripotent cells that are not limited by technical, ethical or immunological considerations. The goal of this proposal is to develop and bank safe and well-characterized patient-specific pluripotent stem cell lines that can be used to study and potentially ameliorate human diseases. Several groups, including ours have recently shown that adult skin cells can be reprogrammed in the laboratory to create new cells that behave like embryonic stem cells. These new cells, known as induced pluripotent stem (iPS) cells should have the potential to develop into any cell type or tissue type in the body. Importantly, the generation of these cells does not require human embryos or human eggs. Since these cells can be derived directly from patients, they will be genetically identical to the patient, and cannot be rejected by the immune system. This concept opens the door to the generation of patient-specific stem cell lines with unlimited differentiation potential. While the current iPS cell technology enables us now to generate patient-specific stem cells, this technology has not yet been applied to derive disease-specific human stem cell lines for laboratory study. Importantly, these new cells are also not yet suitable for use in transplantation medicine. For example, the current method to make these cells uses retroviruses and genes that could generate tumors or other undesirable mutations in cells derived from iPS cells. Thus, in this proposal, we aim to improve the iPS cell reprogramming method, to make these cells safer for future use in transplant medicine. We will also generate a large number of iPS lines of different genetic or disease backgrounds, to allow us to characterize these cells for function and as targets to study new therapeutic approaches for various diseases. Lastly, we will establish protocols that would allow the preparation of these types of cells for clinical use by physicians investigating new stem cell-based therapies in a wide variety of diseases.
Statement of Benefit to California:
Several groups, including ours have recently shown that adult skin cells can be reprogrammed in the laboratory to create new cells that behave like embryonic stem cells. These new cells, known as induced pluripotent stem (iPS) cells should have, similar to embryonic stem cells, the potential to develop into any cell type or tissue type in the body. This new technology holds great promise for patient-specific stem-cell based therapies, the production of in vitro models for human disease, and is thought to provide the opportunity to perform experiments in human cells that were not previously possible, such as screening for compounds that inhibit or reverse disease progression. The advantage of using iPS cells for transplantation medicine would be that the patient's own cells would be reprogrammed to an embryonic stem cell state and therefore, when transplanted back into the patient, the cells would not be attacked and destroyed by the body's immune system. Importantly, these new cells are not yet suitable for use in transplantation medicine or studies of human diseases, as their derivation results in permanent genetic changes, and their differentiation potential has not been fully studied. The goal of this proposal is to develop and bank genetically unmodified and well-characterized iPS cell lines of different genetic or disease backgrounds that can be used to characterize these cells for function and as targets to study new therapeutic approaches for various human diseases. We will establish protocols that would allow the preparation of these types of cells for clinical use by physicians investigating new stem cell-based therapies in a wide variety of diseases. Taken together, this would be beneficial to the people of California as tens of millions of Americans suffer from diseases and injuries that could benefit from such research. Californians will also benefit greatly as these studies should speed the transition of iPS cells to clinical use, allowing faster development of stem cell-based therapies.
Progress Report:
The goal of this project is to develop and bank safe, well-characterized pluripotent stem cell lines that can be used to study and potentially ameliorate human diseases, and that are not limited by technical, ethical or immunological considerations. To that end, we proposed to establish protocols for generation of human induced pluripotent stem cells (hiPSC) that would not involve viral vector integration, and that would be compatible with Good Manufacturing Processes (GMP) standards. To establish baseline characteristics of hiPSCs, we performed a complete molecular characterization of all existing hiPSCs in comparison to human embryonic stem cells (hESCs). We found that all hiPSC lines created to date, regardless of the method by which they were reprogrammed, shared a common gene expression signature, distinct from that of hESCs. The functional role of this gene expression signature is still unclear, but any lines that are generated under the guise of this grant will be subjected to a similar analysis to set the framework by which these new lines are functionally characterized. Our efforts to develop new strategies for the production of safe iPS cells have yielded many new cell lines generated by various techniques, all of which are safer than the standard retroviral protocol. We are currently expanding many of the hiPSCs lines generated and will soon demonstrate whether their gene expression profile, differentiation capability, and genomic stability make them suitable for banking in our iPSC core facility. Once fully characterized, these cells will be available from our bank for other investigators.
For hiPSC technology to be useful clinically, the procedures to derive these cells must be robust enough that iPSC can be obtained from the majority of donors. To determine the versatility of generation of iPS cells, we have now derived hiPSCs from commercially obtained fibroblasts derived from people of different ages (newborn through 66 years old) as well as from different races (Caucasian and mixed race). We are currently evaluating medium preparations that will be suitable for GMP-level use. Future work will ascertain the best current system for obtaining hiPSC, and establish GMP-compliant methodologies.
The goal of this project is to develop and bank safe, well-characterized pluripotent stem cell lines that can be used to study and potentially ameliorate human diseases. To speed this process, we are taking approaches that are not limited by technical, ethical or immunological considerations. We are establishing protocols for generation of human induced pluripotent stem cells (hiPSCs) that would not involve viral vector integration, and that are compatible with Good Manufacturing Practices (GMP) standards. Our efforts to develop new strategies for the production of safe hiPSC have yielded many new cell lines generated by various techniques. We are characterizing these lines molecularly, and have found hiPSCs can be made that are nearly indistinguishable from human embryonic stem cells (hESC). We have also recently found in all the hiPSCs generated from female fibroblasts, none reactivated the X chromosome. This finding has opened a new frontier in the study and potential treatment of X-linked diseases. We are currently optimizing protocols to generate hiPSC lines that are derived, reprogrammed and differentiated in the absence of animal cell products, and preparing detailed standard operating procedures that will ready this technology for clinical utility.
This project was designed to generate protocols whereby human induced pluripotent stem cells could be generated in a manner consistent with use in clinical trials. This required optimization of protocols and generation of standard operating procedures such that animal products were not involved in generation and growth of the cells. We have successfully identified such a protocol as a resource to facilitate widespread adoption of these practices.
Read more
about Functional Genomic Analysis of Chemically Defined Human Embryonic Stem Cell
Functional Genomic Analysis of Chemically Defined Human Embryonic Stem Cell
Funding Type:
Comprehensive Grant
Grant Number:
Julie Baker
Stanford University
ICOC Funds Committed:
$2 628 635
Disease Focus:
Genetic Disorder
Muscular Dystrophy
Stem Cell Use:
Embryonic Stem Cell
Cell Line Generation:
Embryonic Stem Cell
Public Abstract:
Regenerative medicine holds the promise that tissues can be engineered in vitro and then transplanted into patients to treat debilitating diseases. Human Embryonic Stem Cells differentiate into a wide array of adult tissue types and are thought to be the best hope for future regenerative therapies. This grant has three main goals: 1. The creation of new human embryonic stem cells in animal free conditions which will allow for future therapeutic uses. 2. The creation of human embryonic stem cell that contain mutations in their genomes that cause diseases, including cystic fibrosis, muscular dystrophy, Downs Syndrome and many others. These lines can be used to study these diseases and to test potential therapies 3. A close biological assessment of one of the first tissues to arise during differentiation of human embryonic stem cells – the endoderm. Since the endoderm eventually, during many days of development, becomes the pancreas, liver, and gut. It is critically important that we know everything about this very specialized tissue if we are to attempt to engineer these organs in the laboratory. Our overwhelming goal is to provide tools that clinician can use to treat disease whether it is to establish new and improved human embryonic stem cell lines or to find new ways of creating endodermal tissues within the laboratory for future therapeutic uses.
Statement of Benefit to California:
This grant will provide to the state of California new human embryonic stem cell lines that could be used in future therapeutic uses. It will also provide disease specific human embryonic stem cell lines that can be used to study disease and as models to test pharmacological compounds to treat disease. We will also provide a characterization of tissues generated from the new human embryonic stem cells which we hope will someday aid in the formation of liver, lung and pancreas.
Progress Report:
We have made significant progress during the previous granting period which has resulted in a publication in Genome Research detailing genomic DNA methylation changes in a variety of human embryonic stem cells and their derivatives. We have also been successful in identifying regions of the genome bound by of histone modifications and transcription factors in hESCs and derived endoderm. These targets have led to a greater understanding of how Nodal signaling and chromatin configuration maintain pluripotent state and subsequently trigger differentation. We expect 3 publications to result from this work during the next granting period.
Toward a goal of developing endodermal lineages from hESCs, including liver, pancreas, lung and intestine, we have developed new tools and approaches to identify these subtypes as well as a molecular understanding of how these subtypes emerge. These advances are highlighted in three papers which are currently under review for publication and one in preparation. Two of these papers redefine endodermal subtypes derived from hESCs, including new methods to isolate lineage restricted endodermal populations and a means to distinguish between single endodermal cells. The third paper provides an unprecedented view of the Nodal signaling pathway and its intersection with bivalent domains in both hESCs and derived endoderm. This chromatin signature which consists of Smad transcription factors and both histone repressive and active marks is the most conducive for mediating downstream targets of Nodal, providing inroads into how signaling pathways and chromatin cooperate during fate specification in hESCs. This analysis has led to the elucidation of new proteins that mediate Nodal signaling; ones that play a key role in endoderm specification.
During the past year, we have completed three research projects as a result of this grant. Two of these have recently been published in Genes and Development and Developmental Biology. The third is currently in review at Development. The discoveries reported in these papers are highlighted below:
HEB and E2A function as SMAD/FOXH1 cofactors
Nodal signaling, mediated through SMAD transcription factors, is necessary for pluripotency maintenance and endoderm commitment. We have identified a new motif, termed SMAD Complex Associated (SCA) that is bound by SMAD2/3/4 and FOXH1 in human embryonic stem cells (hESCs) and derived endoderm. We demonstrate that two bHLH proteins - HEB and E2A - bind the SCA motif at regions overlapping SMAD2/3 and FOXH1. Further, we show that HEB and E2A associate with SMAD2/3 and FOXH1, suggesting they form a complex at critical target regions. This association is biologically important, as E2A is critical for mesendoderm specification, gastrulation, and Nodal signal transduction in Xenopus tropicalis embryos. Taken together, E proteins are novel Nodal signaling cofactors that associate with SMAD2/3 and FOXH1 and are necessary for mesendoderm differentiation.
Chromatin and Transcriptional Signatures for Nodal Signaling During Endoderm Formation in hESCs
The first stages of embryonic differentiation are driven by signaling pathways hardwired to induce particular fates. Endoderm commitment is controlled by the TGF-β superfamily member, Nodal, which utilizes the transcription factors, SMAD2/3, SMAD4 and FOXH1, to drive target gene expression. While the role of Nodal is well defined within the context of endoderm commitment, mechanistically it is unknown how this signal is manifested at binding regions within the genome and how this signal interacts with chromatin state to trigger downstream responses. To elucidate the Nodal transcriptional network that governs endoderm formation, we used ChIP-seq to identify genomic targets for SMAD2/3, SMAD3, SMAD4, FOXH1 and the active and repressive chromatin marks, H3K4me3 and H3K27me3, in human embryonic stem cells (hESCs) and derived endoderm. We demonstrate that while SMAD2/3, SMAD4 and FOXH1 binding is highly dynamic, there is an optimal signature for driving endoderm commitment. Initially, this signature is marked by both H3K4me3 and H3K27me3 as a very broad bivalent domain in hESCs. Within the first 24 hours, at a few select promoters, SMAD2/3 accumulation coincides with H3K27me3 reduction so that these loci become relatively monovalent marked by H3K4me3. JMJD3, a histone demethylase, is simultaneously recruited to the promoters of the endodermal gene GSC and EOMES, suggesting a conservation of mechanism at multiple promoters genome-wide. The correlation between SMAD2/3 binding, monovalent formation and transcriptional activation suggests a mechanism by which SMAD proteins coordinate with chromatin at critical promoters to drive endoderm specification.
Distinguishing Cells with Housekeeping Transcripts
Distinguishing between cell types is central to a broad array of biological fields and is at the heart of advancing regenerative medicine and cancer diagnostics. In this report, we use single cell gene expression to identify transcriptional patterns emerging during the differentiation of human embryonic stem cells (hESCs) into the endodermal lineage. Endoderm specific transcripts are highly variable between individual CXCR4+ endodermal cells, suggesting that either the cells generated from in vitro differentiation are distinct or that these embryonic cells tolerate a high degree of transcript variability. Housekeeping transcripts, on the other hand, are far more consistently expressed within the same cellular population. However, when we compare the levels of housekeeping transcripts between hESCs and derived endoderm, patterns emerge that can be used to clearly separate the two embryonic cell types. We further compared 4 additional human cell types, including 293T, iPSC, HepG2 and endoderm derived iPSC. In each case the relative levels of housekeeping transcripts defined a particular cell fate. Interestingly, we find that three transcripts, LDHA, NONO and ACTB, contribute the most to this diversity and together serve to segregate all 6 cell types. Overall, this suggests that levels of housekeeping transcripts, which are expressed within all cells, can be leveraged to distinguish between human cell types and thus may serve as important biomarkers for stem cell biology and other disciplines.
Read more
about Spotlight on Muscular Dystrophy and Stem Cell Research - Helen Blau, Stanford
Spotlight on Muscular Dystrophy and Stem Cell Research - Helen Blau, Stanford
Read more
about Tufan Aydogdu, Sanford-Burnham - CIRM Stem Cell #SciencePitch
Tufan Aydogdu, Sanford-Burnham - CIRM Stem Cell #SciencePitch
Read more
about Carrie Miceli | Stanley Nelson, UCLA - CIRM Stem Cell #SciencePitch
Carrie Miceli | Stanley Nelson, UCLA - CIRM Stem Cell #SciencePitch
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Twelve Gauge Valentine
From Wikipedia, the free encyclopedia
|This article is being considered for deletion in accordance with Wikipedia's deletion policy.
Please share your thoughts on the matter at this article's entry on the Articles for deletion page.
Feel free to edit the article, but the article must not be blanked, and this notice must not be removed, until the discussion is closed. For more information, particularly on merging or moving the article during the discussion, read the Guide to deletion.
|The topic of this article may not meet Wikipedia's notability guideline for music. (December 2014)
|This article does not cite any references or sources. (December 2014)
Twelve Gauge Valentine (or 12 Gauge Valentine) is an American Metal Band that started in 2003, which hails from little towns near Monroe, Louisiana. They have made an album and a EP. In 2006, they signed to Sound VS. Silence Records and immediately left to join Solid State Records. In 2008, they broke-up.
- Album
Shock Value
Final Line-up
- Jon Green - Vocals (2003-2008)
- Josh Fife - Guitar (2007-2008)
- Jake Campbell - Guitar (went on to play for Alesana) (2007-2008)
- Jonathon Cooper - Bass (2003-2008)
- Michael Watson - Drums (2007-2008)
- Josh White - Guitar (2003-2007)
- Josh Gross - Guitar (2003-2006)
- Jonathon Webster - Drums (2003-2006)
- Kyle Snellenberger - Guitar (2006-2007)


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Dr. Oswald Regueira, MD
Pediatrics, Texas Tech University Pediatrics
- 1400 S Coulter St
Amarillo, TX 79106
- 1 Office Location
- (806) 414-9800
Dr. Oswald Regueira graduated from the Univ Central Del Este (uce), Fac De Med, San Pedro De Macoris, Dom Republic in 1981. He works in Amarillo, TX and specializes in Pediatrics and Pediatric Hematology-Oncology. Dr. Regueira is affiliated with BSA Hospital and The Pavilion Northwest Texas Healthcare System. He speaks English and Spanish.
Gender: Male
Years in Practice: 33 Years
Languages Spoken: English, Spanish
Medical School: Univ Central Del Este (uce), Fac De Med, San Pedro De Macoris, Dom Republic
Graduation Date: 1981
Hospital Affiliations: BSA Hospital, The Pavilion Northwest Texas Healthcare System
Specialties: Pediatrics, Pediatric Hematology-Oncology