text:
Skip navigation
Main Menu
Utility menu
Stay Connected
Privacy Policy
FTC en español
Search form
Main Menu
About the FTC
What We Do
Enforcement Authority
Our History
FTC Moments
FTC@100 Fireside Chat
Our Seal
Centennial Dinner & Symposium
Edith Ramirez
Julie Brill
Maureen K. Ohlhausen
Joshua D. Wright
Terrell McSweeny
Bureaus & Offices
Bureau of Competition
Bureau of Consumer Protection
Bureau of Economics
Office of Congressional Relations
Office of Equal Employment Opportunity
Office of International Affairs
Office of the Executive Director
Office of the General Counsel
Office of Policy Planning
Regional Offices
Office of Administrative Law Judges
Office of Public Affairs
Office of the Secretary
Bureau and Office Directors
Former Commissioners
Former Bureau and Office Directors
Financial Documents
Other Financial Information
Office of Inspector General
Scam Alert
What You Need to Know About the Office of the Inspector General
Reporting Waste, Abuse or Mismanagement
Whistleblower Protection
OIG Audits and Evaluations
OIG Investigations
OIG Reading Room
Notice Concerning Nondisclosure Policies, Forms, or Agreements
Contact the OIG
About the Privacy Act
FOIA Reading Rooms
FOIA Request
Fee Regulations
Freedom of Information Act Contacts
Frequently Asked Questions (FAQ's)
Table of Contents
Careers at the FTC
Types of Jobs at the FTC
The FTC Benefits
News & Events
Press Releases
Commission Actions
Media Resources
Consumer Finance
Mergers and Competition
Mobile Technology
The Do Not Call Registry
Tools for Consumers
Truth In Advertising
Protecting Consumer Privacy
Identity Theft and Data Security
Military Consumer Protection
Events Calendar
All Events
Weekly Calendar
Weekly Calendar Archive
Featured Videos
FTC Events
For Consumers
For Business
En Español
Social Media
FTC Twitter Chats
General Social Media Chats
Tweeting FTC Events
Competition Matters
Comment Policy
Cases and Proceedings
Case Document Search
Commission Decision Volumes
Closing Letters and Other Public Statements
Petitions to Quash
Adjudicative Proceedings
Premerger Notification Program
Statute, Rules and Formal Interpretations
Form and Instructions
Informal Interpretations
Early Termination Notices
Current Thresholds
Filing Fee Information
Post-Consummation Filings (HSR Violations)
Medicare Act Filings
HSR Resources
Contact Information
First Time Filer?
Merger Review
Anticompetitive Practices
Rulemaking and Regulatory Reform Proceedings
Regulatory Review
Consumer Sentinel Network
Data Contributors
Criminal Liaison Unit
CLU Awards
Contact CLU
CLU Fact Sheet
Amicus Briefs
Advocacy Filings
Other Applications, Petitions, and Requests
Merits Briefs
Advisory Opinions
Cooperation Agreements
Federal Register Notices
Policy Reports
US Submissions to OECD and Other International Competition Fora
Litigation Status Report
Magnuson-Moss Warranty Public Audit Filings
Webb-Pomerene Act Filings
Public Comments
Advanced Comment Search
Policy Statements
International Competition
International Consumer Protection
International Technical Assistance Program
International Cooperation Agreements
Competition & Consumer Protection Authorities Worldwide
International Fellows Program
Tips & Advice
For Consumers
Business Center
Competition Guidance
I Would Like To...
Submit a Consumer Complaint to the FTC
File a Comment
Get a Free Copy of My Credit Report
List a Number on the National Do Not Call Registry
Report An Antitrust Violation
File Documents in Adjudicative Proceedings
You are here
Tag: Hospitals and Clinics
Tag: Hospitals and Clinics
Tag: Hospitals and Clinics
Displaying 21 - 40 of 61 results.
OSF Healthcare System, and Rockford Health System, In the Matter of
The FTC filed an administrative complaint challenging OSF Healthcare System's proposed acquisition of Rockford Health System, charging that the acquisition would substantially reduce competition among hospitals and primary care physicians in Rockford, Illinois, and significantly harm...
OSF Healthcare System and Rockford Health System
DaVita Inc.
The Commission required dialysis services company DaVita, Inc. to sell 29 outpatient dialysis clinics around the United States, under a settlement that resolves FTC charges that DaVita's proposed $689 million acquisition of rival CDSI I Holding Company, Inc., also known as DSI, would...
Phoebe Putney Health System, Inc., Phoebe Putney Memorial Hospital, Inc., Phoebe North, Inc., HCA Inc., Palmyra Park Hospital, Inc., and Hospital Authority of Albany-Dougherty County, In the Matter of
On 4/20/2011, the FTC challenged Phoebe Putney Health System, Inc.'s (Phoebe's) proposed acquisition of rival Palmyra Park Hospital, Inc. (Palmyra) from HCA, in Albany, Georgia. The FTC's administrative complaint alleges that the deal will reduce competition significantly and allow...
Phoebe Putney Health System, Inc.
The FTC challenged Phoebe Putney Health System Inc.'s propoased acquisition of rival Palmyra Park Hospital from HCA, alleging that the deal would likely substantially lessen competition and allow the combined Phoebe/Palmyra to raise prices from general acute care hospitals services...
ProMedica Health System, Inc., FTC and State of Ohio
ProMedica Health System, Inc., a corporation, In the Matter of
On 1/6/2011, the FTC challenged ProMedica Health System, Inc.'s consummated acquisition of rival St. Luke's Hospital in Lucas County, Ohio. The FTC's administrative complaint alleges that the deal will reduce competition and allow ProMedica to raise prices for general acute-care and...
Laboratory Corporation of America and Laboratory Corporation of America Holdings
Laboratory Corporation of America and Laboratory Corporation of America Holdings, In the Matter of
The FTC challenged Laboratory Corporation of America's $57.5 million acquisition of rival clinical laboratory testing company Westcliff Medical Laboratories, Inc., alleging that the transaction would lead to higher prices and lower quality in the Southern California market for the...
Universal Health Services, Inc., Psychiatric Solutions, Inc., and Alan B. Miller, In the Matter of
The FTC required Universal Health Services, Inc., one of the nation's largest hospital management companies, to sell 15 psychiatric facilities as a condition of its $3.1 billion acquisition of Psychiatric Solutions, Inc. As originally proposed the acquisition would have reduced...
Commission Approves Carilion Clinic's Application to Sell Center for Surgical Excellence to Fairlawn Surgery Center, LLC
The Federal Trade Commission has approved a proposed divestiture by Virginia-based health care company Carilion Clinic. Under a December 2009 settlement with the FTC, Carilion must divest a medical imaging center and an outpatient surgical center in Roanoke, Virginia, to FTC-approved buyers. That...
Bureau of Competition Director Issues Statement on FTCs Closure of its Investigation of Consummated Hospital Merger in Temple, Texas
Richard Feinstein, Director of the Federal Trade Commission's Bureau of Competition, has issued a statement on the agency's decision to close its investigation into a consummated hospital merger in Temple, Texas. The Bureau is issuing this statement in the interest of transparency, to explain the...
Commission Issues 2009 Report on U.S. Ethanol Market Concentration; FTC Approves Final Consent Order in Matter Concerning Carilion Clinic
Commission Issues 2009 Report on U.S. Ethanol Market Concentration The Commission has issued the "2009 Report on Ethanol Market Concentration." This is the FTC's fifth annual report on the state of ethanol production in the United States, as required by the Energy Policy Act of 2005. The report...
Commission Order Restores Competition Eliminated by Carilion Clinics Acquisition of Two Outpatient Clinics
Carilion Clinic, a Virginia-based health care company, has agreed to sell two independent outpatient medical clinics it acquired last year to settle Federal Trade Commission charges that the acquisitions were anticompetitive and violated federal law.
Carilion Clinic, a corporation, In the Matter of
The Commission issued an administrative complaint challenging Carilion Clinic's 2008 acquisition of two competing outpatient clinics in the Roanoke, Virginia, area. The complaint alleges that Carilion's acquisition of these outpatient centers eliminated competition for patients in...
FTC Challenges Carilions Acquisition of Outpatient Medical Clinics
The Federal Trade Commission has issued an administrative complaint challenging Carilion Clinic's 2008 acquisition of two outpatient clinics in the Roanoke, Virginia, area. Prior to the acquisition, the Center for Advanced Imaging (CAI) and the Center for Surgical Excellence (CSE) had strong...
FTC Approves Order Dismissing Administrative Complaint Against Inova Health System Foundation and Prince William Health System, Inc.
Commission approval of order dismissing administrative complaint – The Commission has approved an order dismissing its administrative complaint against Inova Health System Foundation (Inova) and Prince William Health System, Inc. (PWHS) without prejudice, as the respondents publicly announced...
Statement of FTC's Bureau of Competition regarding Inova Health System's Announced Withdrawal of Plans to Merge with Prince William Health System
After a thorough investigation, the FTC filed a complaint in the U.S. District Court for the Eastern District of Virginia on May 12, 2008, seeking an injunction to stop the proposed combination of Inova and Prince William hospitals. The complaint alleges that the transaction would be...
FTC and Virginia AG File Complaint in Federal Court Seeking to Block Inova Health System Foundations Acquisition of Prince William Health System
The Federal Trade Commission and the Commonwealth of Virginia filed a complaint in federal court yesterday seeking a temporary restraining order and preliminary injunction to stop Inova Health System Foundation's (Inova) proposed acquisition of Prince William Health System (PWHS) pending a full...
Inova Health Systems Foundation and Prince William Health System, Inc., In the Matter of
« first
‹ previous
next ›
last »
Utility menu
Stay Connected
Privacy Policy
FTC en español
About the FTC
What We Do
Our History
Bureaus & Offices
Office of Inspector General
Careers at the FTC
News & Events
Press Releases
Commission Actions
Media Resources
Events Calendar
Social Media
Cases and Proceedings
Premerger Notification Program
Merger Review
Anticompetitive Practices
Consumer Sentinel Network
Criminal Liaison Unit
Advisory Opinions
Cooperation Agreements
Federal Register Notices
Public Comments
Policy Statements
Tips & Advice
For Consumers
Business Center
Competition Guidance
I Would Like To...
Submit a Consumer Complaint to the FTC
File a Comment
Get a Free Copy of My Credit Report
List a Number on the National Do Not Call Registry
Report An Antitrust Violation
File Documents in Adjudicative Proceedings
Site Information
Privacy Policy
Website Policy
No FEAR Act
Digital Government Strategy
Open Government
Federal Trade Commission
600 Pennsylvania Avenue, NW
Washington, DC 20580
Contact Us
Stay Connected
Stay Connected with the FTC


text:
Kenworth offering expenses paid trip, T880 test drive
Kenworth announced that it will award prize winners with an all-expenses paid trip and tour for two to the Kenworth assembly plant in Chillicothe, Ohio, to test drive the new Kenworth T880.
Rush Tech Skills Rodeo supports service strategy
Service, more than brands or products alone, will drive the success of Rush Enterprises, said W.M. "Rusty" Rush, CEO and president.
Hebe retires, Navistar adds two to board
Navistar announces the retirement of James L. Hebe, senior vice president of North America truck sales for the company, a position he has held since 2008.
US Bank debuts OTR MasterCard
US Bank is introducing a new Over-The-Road credit card that is optimized for fleet management across the country. Kurt Adams, president of corporate payment system for US Bank and Max Fuller, CEO and Chairman of US ...
Navistar reviewing more plant closings
Navistar CEO Lewis Campbell says company is reviewing all operations beyond its core North American truck and parts business to see whether any need to be adjusted, sold or closed as it seeks to increase ...
Cummins Westport announces new natural gas engine
The ISB6.7 G engine will be based on the Cummins ISB6.7 diesel engine and will use Cummins Westport's spark-ignited, stoichiometric cooled exhaust gas recirculation technology.
Nine up for Truck of Year honors
Jack Roberts, executive editor for Overdrive will serve as one of the judges and will be tweeting photos and impressions throughout the day Friday
Polar Service Center moves
Polar Service has moved to a new location in Rosemount, Minn., with eight service bays, 3,800 square-feet of parts inventory and parking for 75 trailers. The location is ASME National Board R-stamp certified and provides ...
Editor's Picks
Stay Connected
Sign up to get Overdrive's daily newsletter.

subset: commoncrawl


text:
Rallying Against the Holiday Retail Blues
We've told you about the anxiety at Barnes & Noble over this upcoming holiday retail season, which Leonard Riggio predicted would be the worst of his 40-plus-year career. But we've also told you about the Indiebound campaign to rally independent booksellers… and they aren't the only ones trying to eliminate the negative.
PW reported earlier this week on Random House's "Books=Gifts" ad campaign, and several book bloggers have banded together to create a "Buy Books for the Holidays" blog. Add a major online promotional effort from M.J. Rose to that mix: As Random was unveiling their banners, Rose outlined a 31-day campaign that promises "a half a billion impressions across some of the most popular blogs," with a reach of over 10 million readers. And she's looking ahead: "We're also planning on doing the same thing for Valentines Day, Mother's Day and Father's Day," she reports.
The heart of her call for proactive solutions for the slowly dwindling book market bears underlining:
"Our industry needs to do more not less in this economy—this is the time to spend money not hold back—we need to shout that books are still reasonably priced as gifts rather than whisper it. We can't just hope consumers get the message."


text:
Advanced Search | Shop by Brand
Return to view Draper 76334 Submersible Dirty Water Pump with Float Switch 5 Metre Lift 7500 Litres Hour Max Flow 400w 240v
Leave your own review for this product...
Showing 1 to 9 of 9 reviews
By Liz baker on 28/09/2013
Arrived on time, plugged in and did the job. Didn't even need to read the instructions - it was that straight forward.
Was this review helpful to you? Yes | No
By adrian jolly on 03/05/2011
Initially did not work and s nut securing the impeller fell off and had to be put on. The problem was sorted in 5 minutes and it has worked brilliantly since. It drained a large pond in no time at all.
an excellent piece of kit.
By David colman on 20/07/2012
Exactly what i wanted, this pump came very quickly, with the minimum of fuss and bother. An excellent purchase.
By Adrian jolly on 31/03/2012
The pump did not work initially. This was because the the screw holding the pump blade on to the main spindle was loose and the blade fell off. Once i had located the problem and sorted it, about 15 minutes later, all was and continues to work well.
By Julian meredith on 12/09/2013
Good product as with all draper tools, quick dispatch and delivery
By George mckay on 02/10/2012
I use this pump to clear my garage pit of water. I opted for the dirty water pump in case of solid bits floating around, and so far its been a great job. My pit is quite deep (approx 2m) and the pump has no trouble pushing the water up to that height, and emptying the pit takes only a few minutes.. Recommended
By Chris on 18/03/2014
The pump works well but the float switch is temperamental.
By Chris on 04/03/2014
The float switch is tricky and let me down a few times - but the pump works well.
By Mark froggitt on 03/07/2013
Excelent pump for a great price. Not rated for constant use.
Tooled-Up.com78 Suez RoadBrimsdownEnfieldMiddlesexEN3 7PSTel : 0844 335 3902


text:
You must log in to access this feature. Sign in with Twellow or Facebook to get started.
By signing in to Twellow, you indicate your agreement to our terms of service.
Simply connect with Facebook and kiss those password blues away,
or go here to reset your password.
Bermuda breaking news, photos, videos, profiles & history with an interactive focus. Updated around the clock...


text:
Photo Craft Kits
Cross Photo Magnetic Craft Kit (1 dz)
This cross photo frame magnetic craft kit is perfect for making religious-themed photo frames for Easter pictures, church pictures, christening or baptism photos and more. All the foam craft pieces are pre-package for individual use. This is a great craft project for young kids. Frames measure 6 1/2" x 5" and leave enough room for a 4" x 2 1/4" photo. Kit comes with enough material to make 12 photo frames.
- Makes 12 completed magnetic photo frames
- Pieces are pre-packaged for individual use
- Complete frame measures 6 1/2" x 5"
- 4" x 2 1/4" photo space
- Kits include instructions and extra pieces
Regular Price: $12.56
Now only: $8.04
Big Top Photo Cards (1 dz)
These cards are a great way to remember a family's visit to the circus. Family members would love to see pictures of a childs first circus, or you can use these cards as a memento to remember a circus themed party.
- 1 Dozen
- 6 1/4 x 8 1/2 Cardboard Photo Frame
- Holds 3 1/2" Photos
God Is Awesome Ornament Photo Kit (1 dz)
Give children a craft project they are sure to love with these God Is Awesome Ornament Photo Kit. Perfect to make in Sunday School or Vacation Bible School.
- 1 Dozen God Is Awesome Ornament Photo Kits.
- Ornament Measures 7 Inches.
- 2 3/8 Inch Diameter with Photo Space.
- Self Adhesive Foam Pieces.
- Instructions & Extra Pieces Included.
Regular Price: $10.84
Now only: $6.87
Color Your Own Up & Away Photo Frames (1 dz)
Make children smile and think of Spring with this Color your Own Up & Away Photo Frame Magnet. Each kit comes with everything kids need to create a cute photo frame that parents will love to put on the fridge!
- 1 Dozen Color Your Own Up & Away Photo Farm Magnets.
- Frame Measures 4 3/4 inches x 7 Inches.
- Photo Space Measures 2 Inches x 2 5/8 Inches.
- Cardboard.
- Includes Magnets.
Regular Price: $9.78
Now only: $7.13
Child Of God Handprint Photo Frame Ornament Craft Kit (makes 12)Make Christmas even more fun all the kids in your Sunday School class with this Child Of God Handprint Photo Frame Ornament Craft Kit. Kids will love making their own nativity ornament to bring home and put on their family's tree. This kit makes 12 fun ornaments using a child's own handprint. Learn More
Regular Price: $13.66
Now only: $9.98


text:
2012 Q2 Commercial Real Estate Market Survey
2012 Q2: See what commercial practitioners had to say about market performance, sales and rental transactions, challenges, and expectations.
Monthly Report
Attached is the month end Membership Report which contains the total number of members by state.
NAR 2011 Annual Report
The 2011 Annual Report explains how NAR "seized the day" in 2011 on behalf of REALTORS®, homeownership and commercial real estate.
Inflation Watch
In the Inflation Watch Series, NAR Research focuses on the price level. We monitor measures of inflation that affect the business of REALTORS® and summarize their impact, highlighting areas of potential concern in a brief, polished presentation. Released monthly, Inflation Watch enables REALTORS® to gain the insight needed to confidently discuss this important market factor with other professionals and clients.
2012 Q1 Commercial Real Estate Market Survey
2012 Q1: See what commercial practitioners had to say about market performance, sales and rental transactions, challenges, and expectations.
NAR Website Traffic Statistics
Find out who visits NAR's websites, realtor.com® and REALTOR.org, and how often. Learn what's important to fellow REALTORS® and the general public plus know realtor.com's® position in the real estate vertical marketplace.
Download 2014 Website Traffic Statistics
July (PDF: 126 KB)
June (PDF: 126 KB)


text:
Search pictures and profiles of Senior Singles near you right now. Discover how online dating sites make finding singles in the United States, Canada, and all over the world simple, safe and fun! Once you browse profiles and pictures start flirting, messaging and connecting with other members of the SeniorPeopleMeet.com community. Search today and find other sexy, intelligent singles for casual dating or a serious relationship in your area.Search Single Senior Men | Search Single Senior Women
A little about me...
About the one I'm looking for...
After countless hours of exhaustive research, and interviews with women of all professions, shapes, sizes, hair color(both natural and otherwise),political and religious affiliations. I now know what it is that women truely crave and need. More than mutual respect, more than honesty,understanding,faithfulness and compassion. It's SHOES! Shoes of all styles and colors, even to the point of having 5 or 6 of the SAME color because " their a different shade" Apparently it's not possible to have too many.
I'd just like to add...
O.K. Girls some of you,and you know who you are, are taking this much too seriously. Please don't e-mail me that I need to start seeing women who aren't so shallow that all they care about is shoes. IT"S A JOKE!!!!;; Lighten up and have some fun with this. Can't we all just get along.
Meet Senior Singles
in your State


text:
From KHOU to Mamma MiaWho can forget Eileen Faxas, former consumer reporter (a.k.a. "Defender") and part-time anchor? In her reports, Faxas introduced the groundbreaking method of using wacky sound effects (second item) and Benny Hill-type sped-up motion (second item) to illustrate how she w ... More >>
To: Jeff Cohen, CC: The entire World Wide Web
Jokes or criticism from the Chron? Nah, it's just fluff for Jus' Stuff
How many times can the Chronicle write the "How to be a Texan" story?
Houston's latest image-boosting campaign gets a big hand from the Chron


text:
Mets Balk Their Way To Loss
It is difficult to tell which highlight from the Mets game was harder to watch Thursday night: Francisco Rodriguez giving up a two-run homer to Brooks Conrad in the 9th inning to tie the game or D.J Carrasco literally allowing the game winning run cross home plate.
Allowing in this case means balking.
With two outs in the bottom of the 10th, Carrasco gave up back-to-back hits to put runners on the corners. The game ended when the Mets pitcher began to lift his left elbow but stopped without stepping off the rubber. The balk was called and Diory Hernandez was able to score without a pitch being thrown.
"Not too many things get to me, but to lose a game like that, man, that's hard to swallow," Carrasco said. "We battled the whole night. You've got to let somebody at least hit the ball or do something else rather than just balk in the winning run. This is going to be a tough night for me."
The Mets fought hard throughout the game but came up a bit short. They were done by 3 heading into the 5th inning but a four run rally gave them the lead. It seemed like it would be a rare series sweep for the Mets once their ace closer took the mind, but it just was not their night.
Every time you don't get it done," Rodriguez said, "it's pretty frustrating and tough to swallow."
It is an unfortunate end to a rather successful road trip for New York and the players and coaches are being sure to look at the positive from the past 10 games.
"Initially, it's tough," Dickey said. "Everybody here is grieving the loss, so there's heads hanging. But when we get on the plane, we will have taken six out of 10 games on the road, and that's a pretty good road trip."Copyright © 2014, Hartford Courant


text:
Earity wireless earbuds feature a little voice control
Let's be honest, if we put the ability to carry audio from a device to our ears to one side, headphone cords are nothing but trouble. When they aren't balled up in a tangled mess, they're catching on objects and yanking the buds out of our ears. While various wireless earphones have emerged to tackle this problem, the team behind Earity is looking to take this convenience one step further with a pair of earbuds that respond to voice commands. Read More
Like many music lovers on the move, OwnPhones founder and CEO Itamar Jobani became frustrated when his off-the-shelf earphones kept falling out during sporty activity. He gathered a team of expert programmers, electronics engineers, 3D modelers and industrial designers and launched a new effort to develop custom-fit wireless earphones. The tailored personal audio throwers will range from simple plugs that stay put when you're out for a run to ornate jewelry that's sure to create an impression. Read More
If you enjoy music on the move, there's a very good chance that you've been troubled by earphones popping out of your ears just as you're getting into the groove. There are high-end earphones or professional in-ear monitors available that require the buyer to pop along to an audiologist and go through an uncomfortable custom fit process to ensure the buds won't keep falling out as you run through the park or jump around the stage. Or you could look into having a custom set of earphones made. This is precisely what Normal founder Nikki Kaufman did, but the significant cost and long shipping delay she encountered prompted her to find another way. She came up with a way to tailor each pair of earphones to the wearer using a free mobile app and a 3D printer. Read More
Earin: The "world's smallest wireless earbuds"
A team of engineers has developed what they are claiming is the world's smallest wireless earbud. In creating Earin, the vision of the team, led by mechanical and design engineer Olle Lindén, was to produce earbuds that not only did away with the messy wires and cables, but fit unobtrusively in the ear to provide a high quality listening experience. Read More
LG has announced a new premium Bluetooth headset optimized for use with its upcoming G3 smartphone. The LG Tone Infinim was developed in collaboration with audio equipment manufacturer Harman Kardon. It promises excellent sound quality and long standby time. Read More
We're going to be seeing a lot of new wearables soon, from large companies, small companies, and everything in between. LG is ready to join the fray, not just with its Android Wear-running G Watch, but also a pair of fitness devices that are a little different from the usual batch of smartwatches and Google Glass rivals. Read More
Pioneer reveals new earphones designed for DJs
It's not always practical for gigging DJs to wrap a pair of hefty cans around their heads when selecting or preparing tracks on a portable music player, smartphone or tablet, but even high-end earbuds might not cut the mustard when it comes to the specific audio needs of today's digital pancake turner. Pioneer's Professional Sound and Visual Division has developed some enclosed inner-ear headphones specially tuned for mixing and playing on the move. Read More
The Dash smart earbuds play back music, and monitor your workout
Today, companies the world over are looking to crack the wearable technology market, especially in terms of fitness tracking. Bragi is another among the lot, but instead of trying to get users to adopt some sort of bracelet or other device to do the tracking, its product, called The Dash, takes the form of something that people already wear – earphones. Read More
Intel presents wearable technology vision
Intel used its keynote presentation at CES to focus on wearable technology, with Chief Executive Brian Krzanich providing a whistlestop tour of wearable devices that the company will be rolling out. Products touched on included a wearable baby monitor, some smart earbuds and an always-on headset. Read More
Asus launches NFC-capable BT 4.0 earbuds
Asus has announced what is claimed to be the world's first Bluetooth 4.0 headset to feature NFC technology. The ergonomically-designed EB50N earphones support one-touch pairing with smartphones and tablets, feature full-range stereo drivers, and boast a long battery life. Read More
- Features
- Reviews
- Aircraft
- Architecture
- Around The Home
- Automotive
- Bicycles
- Children
- Computers
- Digital Cameras
- Electronics
- Environment
- Games
- Good Thinking
- Health and Wellbeing
- Holiday Destinations
- Home Entertainment
- Inventors and Remarkable People
- Laptops
- Marine
- Medical
- Military
- Mobile Technology
- Motorcycles
- Music
- Outdoors
- Pets
- Robotics
- Science
- Space
- Sports
- Telecommunications
- Urban Transport
- Wearable Electronics


text:
Added Aug 16, 2013
Nedre Vats, Norway
This top is based on Burda 06.2009 #102. I've had this pattern for a while, but decided to try it. Since I love orange I had to make it in my favorite color :) I used jersey lining inside to hold the wrapped fabric in place.
Jersey / viscose
Aug 17, 2013, 02.40 AMby kisses
Nice top and lovely colour
Aug 18, 2013, 07.28 PMby Ellen Hatteland
Thank you very much Kisses! I do love colors and I like your projects very much because they are so colorful :)
Aug 16, 2013, 02.05 PMby lomarilou
For sure that's your color! Beautiful top…Bravo!
Aug 16, 2013, 09.26 PMby Ellen Hatteland
Thanks a lot lomarilou :)
Aug 16, 2013, 10.28 AMby bethmaryharley
This is beautiful! And I love the skirt too! The colour really suits you – it's so lovely and bright! :-)
Aug 16, 2013, 11.27 AMby Ellen Hatteland
That's so kind of you to say – Thank you very, very much! :)
Aug 16, 2013, 10.00 AMby leokadia
Ein wunderschönes Top, deine Idee mit diesem "kurzen" Kleiderschnitt ist prima. Und die Farbe steht dir super!! :-))
Aug 16, 2013, 11.27 AMby Ellen Hatteland
Vielen Dank! :)
Aug 16, 2013, 09.53 AMby Oksana Starzhynska
Orange is your colour, you look so bright and cheerful!
Aug 16, 2013, 11.25 AMby Ellen Hatteland
Thank you Oksana ;)!
Aug 16, 2013, 09.21 AMby schnui
I just love this top and the skirt that you have made in orange. You look gorgeous in orange.
Aug 16, 2013, 09.27 AMby Ellen Hatteland
Thank you! It is definitely my favorite color ;)
Aug 16, 2013, 09.12 AMby Mary Athey
this is gorgeous on you ellen, i love orange as well and it suits you. great photos as well x
Aug 16, 2013, 09.27 AMby Ellen Hatteland
Thanks a lot sweet Mary! :)
Aug 16, 2013, 08.53 AMby liliv-1
I love this pattern and I love your top!! Top feature is the color! Orange is perfect on you!
Aug 16, 2013, 09.08 AMby Ellen Hatteland
Thanks a lot! Yes, I do love orange ;)
Add to Favorites 7 people added it
Find out more about our three fabulous courses starting this week!
Backstage Report
Choose from one of 5 popular dresses.
Member Project of the Week
Kick off party dress season with this cute greenery covered dress.
Editors' Pick
See Meg's picks and get them at a special price with an extra discount for Black Friday
Sewing & Techniques
See how Meg made this edgy dress using the Janome Artistic Edge Digital Cutter!
You must allow our "request for permission" request to login to Burdastyle with Facebook.


text:
- Shop
- Support
- Learn
- Explore
- Exhibitions
- Collections
- Library
- Connect
- Collaborate
- Genealogy
- Calendar
- Programs
- Visit
Apprenticeship Program: High School or GED
2014-15 Application Form and Program Summary
The New Bedford Whaling Museum Apprenticeship Program offers an opportunity to high school students who excel academically and express interest in gaining work and college readiness experience while earning a competitive wage. The Apprenticeship program uses museum resources as well as those of regional academic and scientific institutions to increase the knowledge base and practical experiences of high school students in New Bedford while encouraging them to continue their studies upon entering college.
The Apprentice Program of the New Bedford Whaling Museum is open to New Bedford residents in grades 11 and 12, and the GED equivalent, who qualify for free or reduced lunch. The thematic connection linking all aspects of this program is the continuing story of the growth and significance of New Bedford as shaped by its relationship with the ocean and the harvests made on it.
Through this program, high school students are challenged to understand first, the history of the whale fishery, its historical context, the people who were at the heart of the industry, the evolution of New Bedford and the region and its relationship with the ocean, oceanography, the biology of whales, the impact on world commerce, the impact on the environment and the economics of whaling. The Program addresses the questions: How? When? Why? By Whom? With what consequences?
Summer 2013 apprentices in our new Beetle whaleboat, decorated for the Cape Verdean Independence parade.
The Apprentices are introduced to the challenges of interpreting the story of whaling. Through interaction with the staff of the country's leading whaling museum, students are given insight into the day to day operations of a museum: curatorial, marketing, educational programs, community events, preservation, interpretation, research and how these services make the history of the whale fishery accessible to the public. They learn about the various cultures and communities connected by the world's first global economy.
Also, the Apprentices are immersed into the challenges of modern day commercial fishing. Through an introduction to marine biology, oceanography, and the dynamics of the fishing industry, the Apprentices are encouraged to draw on their understanding of the past to inform their concept of the future of ocean harvests: how they should be managed and the impact of mismanagement on the planet.
Apprentices will meet and learn from a variety of experts, participate in hands-on projects and potentially serve as assistants for on-going monitoring programs coordinated by Museum partners. Just as importantly, students will also develop organizing, problem solving and team-building skills. They will also be encouraged to think creatively, develop presentation, storytelling and public interaction skills, and learn how to think on their feet in front of an audience.
Students will be encouraged to write articles about their apprenticeship for their school newspaper, and will contribute to their own Museum blog site. Their writings will also be considered for inclusion into The Bulletin from Johnny Cake Hill, the Museum's journal for its membership. Apprentices will be expected to create a personal journal that will allow them the opportunity to track their learning over the course of the program and reflect on their experiences as they happen. Ultimately, students will exit the program as ocean literate citizens, stewards of these resources, fully cognizant of how 'the ocean and humans are inextricably interconnected.'
The Program consists of two sessions during the academic year and one summer session. The school year sessions will run from September – January and February – May. Students will work from 3:00 pm – 5:00 pm during these sessions, four days a week. The summer session will take place for six weeks during July – August, with students working from 10:00 am – 4:00 pm, four days a week.
Application Process and Period of Work
Application forms are available below (see Application Form), at the Museum's front desk or at your school, through the school staff listed in the next sentence. Completed forms and a copy of the most recent report card can be submitted to: Brian Witkowski, Director of Apprentices and Interns, New Bedford Whaling Museum or to the Dean of Students at New Bedford High School, the Co-op Coordinator at Greater New Bedford Regional Vocational Technical High School, or the College and Career Advisor at New Bedford Global Learning Charter School. Potential candidates will be invited to the Museum for an interview.
Upon acceptance into the program, each student and parent/legal guardian will sign a Letter of Commitment for that academic year and a document promising to apply to college in the future. The Science Director will also sign the Letter of Commitment and provide a copy to the apprentice. Apprentices under the age of 18 will also need to submit a signed Work Permit.
Eligibility: Students must be New Bedford residents receiving free or reduced lunch at school to qualify for the program. Upon hiring, apprentices will submit a signed Income Verification Form, as required by our funders. Compensation: $8/hr.
Application Form (.doc) and Program Summary (PDF) can be downloaded here.
Read the September 12th, 2013 Press Release here: New Bedford Whaling Museum Apprentice Program to receive $128,000 federal grant


text:
Hellooooooooo,,,,, Does google change its profile picture like us ?;-PP
Tags: relationships, careers, computers & internet
Asked by nahu khan, 22 Feb '13 08:45 pm
Earn 10 points for answering
Answers (12)
Yes change is the symbol of dynamism.. Further, they send some message through the changed picAnswered by subhash, 22 Feb '13 09:26 pm
YesAnswered by anil garg, 22 Feb '13 09:15 pm
interesting too
interesting too
You know the boy in my PP was looking for his GF! he wept a lot that he has filled the bath tub..Answered by HARI singh Nalwa, 22 Feb '13 09:14 pm
still he is weeping for her!
can you help him ;-P
still he is weeping for her!
can you help him ;-P
Yes, sometimes it even has amphibians like crocodiles & fishes too :)PAnswered by simon waaras, 22 Feb '13 09:05 pm
HelloooooooooAnswered by Keteshwari Manthapuram, 22 Feb '13 09:05 pm
Yes, we only follow its step na :-PAnswered by Annes, 22 Feb '13 09:02 pm
Yes dont you see ? many times its doodle is changedAnswered by Shatabdi Express, 22 Feb '13 08:50 pm
Yes. We do it like google.Answered by san, 22 Feb '13 08:50 pm
Nice prof pic sir / ma\'mAnswered by Rocking Raaj, 22 Feb '13 08:46 pm
Yes, much faster than u.Answered by MAdhavan Avadhany, 22 Feb '13 08:46 pm


text:
My short take? Mr. Voltaggio is over-extended and Mr. Richard has some really bad business advisers. The Washingtonian and I disagree over America Eats Tavern. If anyone can make a restaurant work in Tysons, it's Jose Andres.
FOOD MATTERS: Urbana emerges today from a $600,000 "refresh" that finds a bigger lounge, Italian-accented cocktails (Negronis on tap!), more gluten-free pizzas and pastas and a private dining room relocated to the lobby area … Ralf Schlegel was recently promoted to the position of executive chef at Plume at the Jefferson hotel, which recently acquired the wine services of ace sommelier Jennifer Knowles, formerly of Fiola Mare and the Inn at Little Washington. The chef, a German native, comes from a family of hoteliers and first worked in Washington as the executive sous chef at Marcel's in the West End … The incoming chef at Zentan is Yo Matsuzaki, who previously cooked at the modern Japanese restaurant Ozumo in Oakland.
Good morning, everyone. Lots to chew over this morning. Let's begin.


text:
Food to calm the monkeys in your house.
This pay period is tight. I mean super tight. I'm scrapping to have gas to get the kids to school. I ran out of yummy treats for their lunch bags. Oh the horror. So yesterday I got cooking and made some baked goodness for them. I made banana bread muffins for breakfast, chocolate chip cookies for the desert and Chunky Monkey Granola Bars for their snack. I'm in love with the Chunky Monkey. I had it for lunch. My sister shared the recipe a few years back. She found it on the internet somewhere, so this is not my recipe. I just thought on this dreary rainy day I would share it with you. Enjoy.
1/4 cup butter
1/4 cup brown sugar
1/4 cup honey
1/4 cup creamy peanut butter or sunflower seed butter
1 teaspoon pure vanilla extract
2 cups quick oats
1/2 cup puffed rice
3/4 cup chopped dried banana chips
3/4 cup cup dark chocolate chunks or chocolate chips
Place an 8-inch by 8-inch square of parchment paper into the bottom of a pan of the same size.
Stir together the oats, puffed rice, walnuts, and banana chunks (break the banana chips up) in a large mixing bowl. Set aside.
In a medium saucepan, melt the butter then stir in the brown sugar, honey, and peanut butter. Bring to a boil. Once it's bubbling hard, let it boil for 2 minutes. Remove the pan from the heat and stir in the vanilla. Be careful, it will bubble up when you add the vanilla!
Immediately pour over the dry ingredients and gently but quickly stir it in thoroughly. Turn it onto the parchment lined pan right away and press into the pan evenly. Sprinkle the chocolate chunks or chocolate chips over the pan and let stand, undisturbed, until the chocolate looks shiny, about 5 minutes. When the chocolate looks very shiny it should all be melted and can be spread evenly with a silicone/rubber spatula. Let stand until the chocolate has firmed back up.
Run a knife around the edge of the pan to loosen any chocolate that stuck to it, then turn the large block of granola bar onto a cutting board. Flip it chocolate side up and cut into bars of your desired size. Store in an airtight container at room temperature for up to a week.


text:
This is the first hotel I was in India. Went in without reservation and was correct but not impressive. Personally I would´t recommend it but I would´t tell someone not to go either. Average. Despite this, Lallu, the guy in the reception was so helpful and polite!
- Also Known As:
- Greenland Lodge Kerala, India


text:
Michelle Anderson has extensive experience providing SEO and SEM services in a variety of industries, including the extremely competitive timeshare and car dealership arenas. She has served on the educational committee for SEMPO, the Search Engine Marketing Professionals Organization, and is a volunteer with the Analysis Exchange – helping to educate the next generation of search engine professionals.
In addition to being an expert in pay-per-click advertising, she is also Google Analytics Certified, Landing page Optimization Certified and Inbound Marketing Certified. She has been published in more than a dozen publications including newspapers, magazines and books. She brings great ideas and energy to The Marketing Zen Group.
You can find Michelle on Google+, LinkedIn, and Twitter.
Whether you are looking for a capable, full service marketing team to handle all your digital marketing efforts or for piecemeal services, like a social media marketing campaign, or a better converting website – you are in the right place. Contact us via the web form or call us at 1.888.460.6008 today!


text:
Search Results: 'unpredictable'
Paperback: $11.44
Ships in 3-5 business days
this book is about a teen called Sam who is tackling some issues in her life. during the book she seizes the heart of school heart throb and bad boy Jake but things become dangerous for them both.
Unpredictable Destiny
Paperback: $15.56
Ships in 3-5 business days
Unpredictable Destiny describes lives of the two poor girls, Darina and Emily, who grew up in the small town and became very successful. They dreamed to become artists in a big city, but were sure... More > that their dreams cannot be realized due to hard life. The girls did not predict that becoming hostages of the two dangerous criminals could make their dreams became true. The book is intended for the wide range of readers who like adventure stories.< Less
Life's Unpredictable...
eBook (PDF): $0.00
Download immediately.
No description supplied
Life's Unpredictable...
Paperback: $6.22
Ships in 3-5 business days
No description supplied
WEATHER'S UNPREDICTIBILITY Illustrated with Pictures and Poems
Paperback: $18.95
Ships in 3-5 business days
This book shows the unpredictability of weather and how it affects us and our lives. We never know when we will be faced with unimaginable destruction or coping with weather on a daily basis. Each... More > season has its own set of rules and we have to heed to the signs or have to accept the consequences. -Anita DeMeulenaere< Less
9 Stories of Mom's In Unpredictable Sex Situations
eBook (ePub): $3.99
Download immediately.
Here are 9 Mom stories of mothers in unusual sex situations! 1. MIA PUNISHES HER MOM HARD 2. A MOTHER AND SON GAME 3. MOM AND THE BULLY 4. A MOTHER AND SON REAL WORKOUT 5. MOM'S... More > MISSING PANTIES 6. A LITTLE TIED UP FOR MOM 7. GLORIA'S GAG 8. A BIRTHDAY COLLAR FOR MOM 9. WHAT HAPPENS AT CATHY'S HOUSE IS A SECRET< Less
12 Stories of Mom's in Unpredictable Sex Situations!
Paperback: $11.99
Ships in 3-5 business days
1. Shaved by Aiko. 2. A Lesson in Bondage. 3. Sarah Dents the Car. 4. Mom Catches Daughter with another Girl. 5. Lori Experiments with Her Passed Out Mom. 6. Katie Seduces Mom. 7. Brian Catches His... More > Mom with the Pool Boy and Blackmails Her. 8. Mom's New Sexy Work Out Gear. 9. Mom's New Date is a Girl. 10. Mom Smoothers Son. 11. Mom Gets Toy Stuck. 12. Mom Finds Me Tied Up.< Less
12 Stories of Mom's in Unpredictable Sex Situations!
eBook (ePub): $2.99
Download immediately.
Have fun reading these 12 different stories of mom's in unusual sexual situations, and what they have to do! 1. Shaved by Aiko. 2. A Lesson in Bondage. 3. Sarah Dents the Car. 4. Mom Catches... More > Daughter with another Girl. 5. Lori Experiments with Her Passed Out Mom. 6. Katie Seduces Mom. 7. Brian Catches His Mom with the Pool Boy and Blackmails Her. 8. Mom's New Sexy Work Out Gear. 9. Mom's New Date is a Girl. 10. Mom Smoothers Son. 11. Mom Gets Toy Stuck. 12. Mom Finds Me Tied Up.< Less
The Asteroid
Hardcover: $19.99
Ships in 6-8 business days.
An asteroid lands in a corn field. The farmer's nephew touches the rock and becomes possessed at night time. He haunts the house after he kills everyone inside of it. Thirty years later, three... More > children, Chris, Fred, and Gary, are having a camp out in Chris' Back-yard. Chris insists that they to go inside the mysterious house. When they all walk into the house and the door slams shut behind them.< Less
Paperback: $16.50
Ships in 3-5 business days
Top 10see more >
Referral Mastery
Paperback: $13.32
The Client Centered...
Paperback: $5.40
How I made it: Striking...
Paperback: $16.10
More From The Other Side...
Paperback: $300.00
Turn Your iPad into a...
Paperback: $18.94
Blood in the Studio
Paperback: $14.99
A Stone Lives On
Paperback: $19.00
Common Sense: A Book...
Paperback: $9.98
The Best of Trent
Paperback: $20.00
Beautiful Chaos
Paperback: $15.00


text:
Grainger appoints Ian Coull as non-exec director
Related fund data links
Ian Coull is to join the board of Grainger as a non-executive director on 23 September 2014 and will be proposed as chairman at the AGM in February 2015.
Robin Broadhurst will retire as a director and as chairman on that day.
Coull (pictured) has been chairman of Galliford Try since 2011. He was chief executive of SEGRO from 2003 to 2011 and prior to this, a main board director of J Sainsbury. He has also been a non-executive director of Pendragon, a role he held from December 2010 to January 2013.
He is currently a non-executive director of London Scottish International and of The Duchy of Lancaster and is also a member of the government's property advisory panel.
Broadhurst says: "Ian has enjoyed a distinguished career in the property sector embracing considerable real estate experience both within the UK and internationally. He is well known and liked within the industry and the board supports me in believing that his leadership style will be a great asset to Grainger and help take forward the company's strategy in the next stage of its growth."
- News
- By Category
- News from other sites
- Education
- Special Reports
- Guides
- Events
- Partner events
- Awards
- Directory
- Jobs


text:
Properties in Pen Elm, Taunton, Somerset between £30,000 and £2,000,000
- News feed
- Find us on Twitter
Follow us for the latest news
- Find us on Facebook
Dorset Echo
Like us on Facebook
Properties in Pen Elm, Taunton, Somerset
Gallery view List view Map view 3 properties found
- Print Page
- Share Results
To provide you with the best possible service we only show properties for which estate agents have provided full and valid postcodes. You may find more properties in our List or Gallery views.
£499,950 5 Bedrooms Glen Cottage, Langford Lane, Pen Elm, Taunton, Som
A 5 bedroom detached period cottage standing in large gardens extending to about 0.57 acre including covered swimming pool and tennis court
Chesterton Humberts - Taunton
£795,000 Sold STC 5 Bedrooms Mill Barton, Fitzroy, Norton Fitzwarren, Taunton,
A stunning detached country house with generous and versatile three storey accommodation set in gardens and grounds of approximately 3.25 acres in this delightful Quantock Foothills situation
Chesterton Humberts - Taunton
£795,000 5 Bedrooms Fitzroy, Taunton, Somerset
5 Bedrooms
1.65 acres
Living Room
Dining Room
Sitting Room
Kitchen/Breakfast Room
Utiltiy Room
Walk In Larder
En-Suite Shower Room
1.65 acres
Living Room
Dining Room
Sitting Room
Kitchen/Breakfast Room
Utiltiy Room
Walk In Larder
En-Suite Shower Room
Greenslade Taylor Hunt - Taunton
- Print Page
- Share Results
Moving House?
Local businesses here to help you:
- Banks and Building Societies
- Car & Van Hire
- Electrical Appliances
- Flooring
- Furniture
- Insurance Services
- Mortgages
- Removals
- Solicitors
- Storage
- TV Aerials
- All Businesses
Local advertisers


text:
Community Information Database
You are browsing the Subject Heading Job Finding Assistance which contains 13 record(s)
- The Santa Cruz Neighborhood Career Center
Located at the Goodwill Main office. Free and open to the public. Computers, internet, fax, phones, job board, vocational counselors, resume help, job search services and resources to the community. ... [Full Record] [Add to Personal List]
- Women Ventures Project
Women Ventures Project, or WVP, provides women with the information, skills, and support that they need to enter into living wage, nontraditional green and higher wage employment. Call ahead for an... [Full Record] [Add to Personal List]
- Workforce Santa Cruz County Full Service Career Center at Watsonville
One-stop shops for an array of services for employers, employees, job seekers and providers of educational and career counseling services. The Career Centers offer universal access to core employment ... [Full Record] [Add to Personal List]


text:
The Press Newspaper
The Press Newspaper
'Mount Trashmore'
Out West, we have the beautiful monument to past presidents called Mount Rushmore. In Northwood, we have the smelly monument of trash that I will call Mount Trashmore.
This is the morning of July 12 – it is the second delightful day with low humidity, and the morning is just beautiful. We had 16-17 days of constant every-day rain, so it is just great to have the windows open all night and not have to worry about wet carpets.
But wait, as I begin to wake up about 6:30 a.m., I can tell immediately the direction of the wind this morning without lifting my head. It is from the north. You may ask, how I know this. The air in my room has that putrid smell of Mt Trashmore ("The Mount") – the Northwood landfill and mound of trash.
What a wonderful way for all of us living beneath this mound of trash to exist – every sunset is destroyed, and almost every day has that distinct smell as you drive I-280. Lucky me, the winds do not come from the north very often but pity the folks to the East of "The Mount."
No wonder Woodville Road has become an area of vacant buildings – Woodville Mall has become a thing of the past and will probably be torn down. People downwind cannot sell their homes. Who would want to live and work downwind from "The Mount?"
How high does this go? There is no end to it. It is probably about time to raise the maximum height again so we can continue the fill of "The Mount." Now we have construction of a road worth millions so we don't block the flow of trucks dumping more trash into "The Mount."
Is there progress being made here? Is there any governmental organization that can control this nightmare for the folks beneath "The Mount?"
Last fall, the Ohio Democratic Party, led by Chris Redfern, campaigned against past commissioner Mark Stahl, condemning him for raising taxes without asking the voters for their opinion. After their candidate, Jodi Regal, was elected, she, along with the other Democratic commissioner, immediately did exactly what her party boss had declared to be inappropriate – they passed a permanent tax increase, even though the economy is now improving.
This situation is just another example of Redfern's tactics. He promotes one position to get his candidate elected and then has his party minion do just the opposite. What a surprise.
You must conclude you cannot trust anything Redfern says. Now we will be paying more every time we go to the store, on a permanent basis. We have two Democratic commissioners to thank for that. These local politicians seem to follow the party line by taking temporary emergency tax increases and then making them permanent without regard for the electorate's wishes.
Habitat is currently building a home for a family in the village of Oak Harbor. We would like to thank the Foundation for their continued support.
First, let's take a look at the shooting in the movie theatre inside Aurora, Colo. As is with most businesses, there was and still is a sign proclaiming that firearms are not allowed within the facility. Did this sign or any law passed by either the Colorado government or the U.S. government stop James Eagan Holmes from entering the theatre on that night and killing 12 people while injuring 70 others? The answer is a simple no.
Let's also examine the temple shooting in Wisconsin. In August of 2012, Wade Michael Page killed six people and injured four others. Did the sign outside the Sikh temple telling people not to bring a gun into the facility stop the shooter from going in and forever altering 10 families' lives? Yet again we stumble across the answer – no.
The point I'm trying to make is that if a criminal wants to bring a gun into an area and cause harm to others, they will. No sign posted anywhere in a building will defend the people inside of a building. Rep. Maag understands this.
The issue at hand on whether to allow guns in churches is not an issue of what political party someone belongs to.
Instead, it is an issue of safety. It's an issue of keeping people safe. We as a state must understand that times are changing. No place is as safe as it was 15 or even 10 years ago.
We must get this issue passed, so people can feel safer within their place of worship.
No results found.


text:
Please login to get more out of this site
You are currently not logged in, do so now to add comments, like articles as well as photos, submit to competitions, translate articles and gain access to unlimited photo upload! If you are not registered yet, go here to find out more about Lomography and join now! If you just want a limited account for now you can use Twitter or Facebook. We would love to have you as a member here!
2 people like this photo
Liking photos helps us to present the best ones to you and others. 2 people like this photo. 2 people like this photo: pangmark and samguino.
No comments yet, be the first
Giochi dementi
Novembre 2011This photo was shot on film. Taken by insidetheeyes with a Holga 120 GCFN loaded with Kodak 200 film in Rome, Italy. These tags describe this photo: full, frame, flash, giochi, game, and play. Date: 2011-11-08. Time: Night. This photo can also be found in the album Holga.


text:
CAPE CORAL, Fla- Cape Coral police identifed the teenager killed while riding his bike in the 600 block of Trafalgar Parkway as 15 year old Cameron Ford. Ford and another bicyclists were riding east towards Skyline in the inside lane when 19 year old Jacob York switched lanes. York did not see Ford on his bike and the two collided.
A traffic homocide investigation is underway although officers say there are no signs of any impairment involved. York was not hurt. Ford died at the scene. Police say he was dark clothes but no helmet. His bike also did not have the required lighting or reflectors.
Ford is Cape Coral's 7th traffic fatality of the year. The last four victims were all under 21 years old.


text:
All News & Blogs
E-mail Alerts
In his final legislative session, McDonnell advocates big changes in transportation and K-12 education
Visit the Photo Place
"A reasonable use of tolls is a proper way to build transportation infrastructure. We've used it very sparingly in Virginia, overall. It is used widely around the country and around the world," McDonnell said. "I do understand the citizens' concerns. If you have a free road now and you're asked by the state to pay a toll, of course you're going to have a concern. But I'm more concerned about the safety improvements that need to be made on I-95, and at this point I am generally out of options. The General Assembly has not provided the resources that I've asked for and that are needed to properly maintain and construct road infrastructure."
McDonnell has also already announced some K-12 proposals--raising teacher pay by 2 percent but linking it to changes in the teacher probation and evaluation process. McDonnell says he'll also have other education bills on issues like school choice. Transportation and education reform, he said, are his top two priorities for the session.
"I looked at this session and said, 'What are some of the major structural problems facing Virginia that we have not fixed?'" McDonnell said. "We'll have a hundred other bills, but those are the things that I think going forward if we do those right and get those done, that will lay a great foundation for future jobs and future opportunity."
McDonnell can set his agenda for his last session, but current events are bringing other issues to the fore. The recent school shootings in Newtown, Conn., have prompted McDonnell to create task forces to study school safety and mental health. And individual lawmakers are planning to propose changes to the state's gun laws.
McDonnell got widespread attention last week for saying, in a radio interview on WTOP, that the possibility of allowing teachers or school staff to carry guns should be considered.
During The Free Lance-Star interview in his office, he said that he was just answering a question, and that he's not advocating any policy changes at this point.


text:
|Table of Contents
|Vol. 45, No. 5, 2008
Issue release date: August 2008
|Section title: Research Paper
Antisense to Transforming Growth Factor-β1 Facilitates the Apoptosis of Macrophages in Rat Vein GraftsHeaton N.S. · Wolff R.A. · Malinowski R.L. · Hullett D.A. · Hoch J.R.
W.S. Middleton VA Hospital and the University of Wisconsin School of Medicine and Public Health, Madison, Wisc., USA Corresponding Author
Background: The success of peripheral vein grafts is limited by intimal hyperplasia. Transforming growth factor (TGF)-β1 has effects on cell proliferation, apoptosis and extracellular matrix synthesis. We have previously observed positive changes in vessel healing with antisense to TGF-β1. Methods: Adenovirus was used to transduce rat femoral artery vein grafts with antisense to TGF-β1 (Ad-AST) or the sequence encoding the bioactive form of TGF-β1 (Ad-BAT). Grafts were harvested at 1, 2, 4 and 12 weeks and formalin fixed for immunohistochemical studies of the cell markers proliferating cellular nuclear antigen (proliferation) and active caspase 3 (apoptosis). In situ DNA fragmentation assays were also performed to confirm active caspase 3 results. Results: Ad-AST treatment significantly (p = 0.05) increased apoptosis of macrophages inside the internal elastic lamina. In addition, Ad-AST treatment significantly increased the cellularity of the graft at early time points and reduced it at later time points (p = 0.01). Conclusion: The low levels of TGF-β1 in Ad-AST treatment promote apoptosis of macrophages and provide an environment that is more conducive to the proliferation or infiltration of cells that contribute to healthy vessels.
© 2008 S. Karger AG, Basel
Chronic arterial occlusion is a common condition in aging patients which leads to intermittent claudication, rest pain and tissue loss. Peripheral vein grafting is the preferred method for bypassing arterial occlusion, and the use of autogenous vein has been shown to improve patency results compared with artificial materials [1]. However, even with the use of autogenous vein, this grafting procedure has had reported failure rates of 50% and higher [2, 3]. This high failure rate is due to excessive remodeling of the vein to cope with arterial pressure leading to the development of intimal hyperplasia (IH) [4].
IH is the major cause of vein graft failure and is characterized by the proliferation of cells and the accumulation of extracellular matrix. This process results in the progressive reduction of the open lumen area. No single cause of IH has been identified, but it has been suggested that the process begins as a healing response that evolves into an unregulated cycle of cellular proliferation and extracellular matrix deposition.
Cytokines and chemokines, which act as cellular regulators, have long been implicated in the formation of IH. Therefore, they are logical targets for gene therapy to attempt to control the healing of the graft and prevent eventual failure [5]. The explanted living tissue used in autogenous vein grafts provides a target for gene therapy ex vivo which avoids the risks associated with systemically exposing the patient to the treatment [6]. This form of gene therapy is practical and hence has a high likelihood of translation into clinical practice if it proves efficacious [7].
Transforming growth factor (TGF)-β1 is a cytokine that has powerful effects on inflammation and wound healing as well as on normal growth and development [8]. It is known to regulate cell growth, apoptosis, cell differentiation and extracellular matrix synthesis [9]. Increased TGF-β1 levels have been measured in human atherosclerotic plaque, restenotic arteries and other fibrotic diseases, such as systemic sclerosis and liver cirrhosis [10, 11]. The specific effects of this cytokine can vary depending on concentration as well as on cell type with the added complication that other signaling pathways can be initiated by TGF-β1. In light of these facts, TGF-β1 involvement in fibroproliferative processes is poorly understood as a whole [12].
Several cell types have been implicated in the healing of vessel trauma and in the development of IH. Macrophages are a key cell type that are protected from apoptosis by TGF-β1 [13], as well as being major factors in the development of IH [14] and important in recruiting additional cells to the area of the trauma [15]. Myofibroblasts are another cell type important in the inflammation response that are stimulated to deposit extracellular matrix in response to TGF-β1 [16, 17]. Mature smooth muscle cells and endothelial cells, which are indicative of a healthy and mature vessel, undergo apoptosis in response to high levels of TGF-β1 [18, 19]. Here, we consider the possibility that TGF-β1 is a key factor in tipping the scale from a positive and desired mode of healing to an unhealthy fibroproliferative one.
Although TGF-β1 expression is detectable by PCR in cDNA from epigastric vein, in our rat model of vein grafting, 4 h after surgery, there is a many-fold increase in the amount of TGF-β1 mRNA [20]. The mRNA expression peaks at 2 weeks with a gradual decline through 4 and 12 weeks, but even at 12 weeks, the TGF-β1 mRNA expression is still greatly elevated over the expression that was seen in the unoperated epigastric vein. TGF-β1 protein is found in two forms, an inactivated form associated with the TGF-β1 latency protein and an active form cleaved from the latency protein. In the natural course of maturation of the grafted vein, both these forms peak at 4 weeks after surgery [21]. In our previous work, we showed that adenovirus-mediated antisense to TGF-β1 RNA treatment was able to knock down TGF-β1 mRNA production and protein levels in rat femoral artery vein grafts and reduce IH formation [21]. Throughout the 12 weeks of our studies, the antisense treatment results in an approximately 90% reduction in the expression of the latent form and a 60–75% reduction in the bioactive form of TGF-β1. We have also shown that reducing TGF-β1 levels reduces the amount of collagen and modifies the cellular composition of the graft [22]. We hypothesize that the positive changes in vessel healing previously observed can be at least partially attributed to the proliferation and apoptosis of cell types linked to desired and undesired routes of vein healing, respectively.
Materials and Methods
Rat epigastric vein-to-femoral artery interposition grafts were placed and harvested in retired breeder male Lewis rats, as previously described [21]. Grafts were harvested at 1, 2, 4 and 12 weeks after placement. Patency rates of harvested grafts were greater than 95% with non-patent grafts due to technical errors during the grafting process. All animal care complied with the Principles of Laboratory Animals (National Society for Medical Research) and the Guide for the Care and Use of Laboratory Animals (National Academy Press). Experimental procedures were approved locally by the Research Animal Resource Center at the University of Wisconsin-Madison.
For the adenoviral gene therapy, 8 mm of freshly harvested, cannulated and irrigated epigastric vein was placed in an adenoviral solution (107 PFU/ml) for 20 min ex vivo. The 3 adenoviral treatments were: (1) infection with adenovirus expressing the mRNA antisense strand to TGF-β1 (Ad-AST), (2) infection with adenovirus that did not express a gene (Ad-CMVpLpA), and (3) infection with adenovirus expressing the mRNA sense orientation of the TGF-β1 gene (Ad-BAT). The Ad-AST construct expressed the TGF-β1 protein coding region in the antisense orientation (coding region with 130 bp of the 5′ untranslated region, 1,303 bp total). The Ad-BAT construct contained a modified TGF-β1 sequence so that the bioactive form of the TGF-β1 protein was expressed and did not require posttranslational modification [23]. Each construct used the cytomegalovirus promoter.
At the appropriate time point, each vein was pressure fixed with buffered formalin in vivo, removed from the animal and then placed in additional formalin overnight for additional fixing of the tissue. The fixed tissue was then embedded in paraffin with the distal anastomosis of the graft away from the cutting side of the block.
The vein tissue was sectioned starting 3 mm from and cutting toward the distal anastomosis. Data from four 5-μm sections 800 μm apart were averaged per vein due to the eccentric nature of IH formation. Antigen retrieval (1 h at 96°C in 1× TE) was performed before blocking nonspecific antibody binding with 2% bovine serum albumin in phosphate-buffered saline. Sudan Black (0.3% in 70% ethanol; Sigma) was used to block the natural autofluorescence of the tissue.
Monoclonal antibodies diluted in 0.2% bovine serum albumin in phosphate-buffered saline were applied to the tissue: proliferating cellular nuclear antigen (PCNA, 2 μg/ml, murine IgG2A, Invitrogen P8825) and active caspase 3 (1 μg/ml, rabbit IgG, R&D Systems AF835). The PCNA antibody bound the nuclei of cells that were actively dividing, and the caspase antibody bound the active form of the protein which is an integral part of the apoptotic cascade. Fluorescent secondary antibodies were applied to the tissue: Alexa fluor-568 (5 μg/ml, goat anti-mouse IgG2A, Molecular Probes A21134) and Alexa fluor-488 (5 μg/ml, goat anti-rabbit, Molecular Probes A11034). Tissue was mounted in Prolong Gold with DAPI (Invitrogen S36939) to help prevent fluorescent tag degradation under UV light. Total cellularity of the graft was determined by DAPI staining.
Apoptosis data from the active caspase 3 stain was verified qualitatively using an in situ apoptosis detection method that enzymatically labeled nicked chromosomal DNA (Trevigen). DNA fragmentation is a well-established marker of apoptosis and a direct downstream effect of the caspase-3-activated DNase [24]. The in situ DNA fragmentation assay also shows that the active caspase 3 results cannot be attributed to an artifact of the immunohistochemical process.
Images of the vein cross-sections were captured by using a Nikon (Tokyo, Japan) Eclipse E600 microscope and Olympus (Melville, N.Y., USA) DP70 color camera. Areas in the image that stained red, green or blue were measured using MetaMorph image analysis software (Universal Imaging, Downingtown, Pa., USA). For each stained color, a range of pixel intensities specific to the stain were defined so that no unstained cells would be measured. Once the range was determined for each color, the parameters were saved in the program and used to determine the colored areas with no further input from the operator. On each image, the internal elastic lamina was manually traced by the operator to define the boundary between the neointima and the media of the vein. Since the media and adventitia merge as the vessel remodels, these layers were grouped as the media/adventitia.
Due to the eccentricity of the vein remodeling process, the 4 images per vein were averaged to give representative values for the entire vein. For each time point, an average of all grafts (n ≥ 5) was calculated. Rat assignment to each treatment group was randomized and the staining of the tissue sections was similarly randomized to insure that statistically significant differences observed were due to the experimental treatment.
The statistical analysis was performed using SAS statistical software (SAS Institute, Inc., Cary, N.C., USA). p values ≤0.05 were considered significant. The 3 treatments were compared across time using an analysis of variance (ANOVA) model. Data were log transformed before p values were calculated to better meet the assumptions of the ANOVA. The general linear models procedure was used to test the main effects (time and treatment) and their possible interactions. If there was a significant interaction effect, the treatments were compared at each time point. If there was no significant interaction between time and treatment, the main effects (time and treatment) were analyzed. Values are expressed as the mean ± standard error of the mean.
As previously reported [22], manipulating TGF-β1 levels in the vein changes the types and ratios of cells present. To determine which specific types of cells were proliferating and undergoing apoptosis, images from the current study and those used to determine cell type previously were compared. Any areas that consistently appeared to overlap either proliferation or apoptosis markers with specific cell types across a treatment were noted. Additional stains for specific cell type and either PCNA or caspase were then performed on slides representative of the treatment groups to confirm proliferation or apoptosis of the specific cell types. Cell-specific markers were: actin smooth muscle-1 (ASM-1, 1.25 μg/ml, Sigma F3777), h-caldesmon (15 μg/ml, Sigma C4562) and macrophage (5 μg/ml, Serotech MCA341R, clone ED1 against the rat homologue of CD68). ASM-1 antibody was conjugated FITC. For h-caldesmon and ED1, Alexa fluor-568 IgG1 was applied as the secondary antibody at 5 μg/ml.
There was a significant IH area/cell number difference between the treatments (determined by the DAPI stain and published previously). To account for those differences, we divided the total area staining positive for PCNA and caspase by the total area of nuclei (fig. 1). After controlling for variation in the cellularity of the grafts, statistical significance was seen at 4 and 12 weeks for PCNA and at 2, 4 and 12 weeks for caspase. Specifically, the Ad-AST treatment reduced proliferation at 4 weeks but increased it at 12 weeks. Ad-AST increased active caspase 3 activity at 4 and 12 weeks. In contrast, Ad-BAT increased active caspase 3 activity at 2 weeks (with a trend toward increased caspase activity at 1 week).
|Fig. 1. Statistically significant differences between treatments remain after controlling for cellularity of grafts. Ad-AST treatment decreased proliferation at 4 weeks and increased proliferation at 12 weeks. In addition, Ad-AST treatment increased active caspase 3 at 4 and 12 weeks. Values are the ratios of PCNA and active caspase 3, respectively, divided by the total area of nuclei. All values are means ± SEM. p values represent differences between treatment groups across all time points. a p ≤ 0.05 versus Ad-CMVpLpA; b p ≤ 0.01 versus Ad-CMVpLpA; c p ≤ 0.05 versus Ad-BAT; d p ≤ 0.01 versus Ad-BAT.
To further dissect the differences between treatments, measurements were made using the internal elastic lamina to separate proliferation/apoptosis in the neointima from proliferation/apoptosis in the adventitial/medial layer (table 1). Both Ad-AST and Ad-BAT treatments had the most significant effects in the neointimal layer.
|Table 1. Vein graft cross-sectional area staining positive for PCNA, caspase and nuclei (DAPI)
At 1 week, in the neointima, the Ad-AST treatment group had significantly higher PCNA and active caspase 3 than the Ad-CMVpLpA control group. However, the Ad-AST also had approximately 16-fold more cells than Ad-CMVpLpA (table 1, nuclei). In the medial/adventitial layer, at the same time point, there was a trend toward increased PCNA and active caspase and an approximately 2.5-fold increase in cells with Ad-AST treatment. Vein grafts treated with Ad-BAT showed only an increase in cell number in the neointima at this time point.
At 2 weeks, in the neointima, active caspase 3 peaked in the Ad-BAT group and was significantly higher than in the empty vector group.
At 4 weeks, in the neointima, both caspase and PCNA were lower in Ad-AST than in Ad-BAT. Also, Ad-AST was lower in PCNA than in Ad-CMVpLpA. At this time point, there was a significant reduction in PCNA in the medial/adventitial layer by Ad-AST.
At 12 weeks, in the neointima, PCNA and caspase were again higher in Ad-AST than in both Ad-BAT and Ad-CMVpLpA.
As opposed to the complex trends seen with proliferation and apoptosis, the amount of cells in the graft followed a simpler progression over time (table 1, nuclei). In the medial/adventitial layer, the Ad-CMVpLpA control and Ad-BAT treatment started with low cellularity at the 1-week time point. Both showed a steady increase until they peaked at 4 weeks and then dropped slightly at 12 weeks. Overall, the Ad-AST treatment caused higher cellularity levels for the first 2 weeks and then caused lower cellularity levels at the 4- and 12-week time points. In the medial/adventitial layer, at every time point, the Ad-AST treatment had significantly different (higher or lower) levels of cells present in the graft compared with the Ad-BAT treatment. In the neointimal layer, the 4- and 12- week time points showed Ad-AST with a lower level of cells than Ad-BAT.
In addition, in both the medial/adventitial and the neointimal layer, at 1, 4 and 12 weeks, Ad-AST had a significantly different (higher or lower) level of cells present in the graft compared with the Ad-CMVpLpA control.
When stained for caspase and PCNA at 1, 2, 4 and 12 weeks, no location or cell type difference emerged between the treatments except for macrophage apoptosis at 4 and 12 weeks in the Ad-AST-treated grafts. At 4 and 12 weeks, cell types were no longer randomly dispersed throughout the graft and were mostly confined to one region of the vessel. At these time points, cells expressing CD68 (macrophages) were located at or near the internal elastic lamina (on the lumen side) in all 3 treatments. Most of the macrophages contained active caspase 3 in the Ad-AST treatment while few if any macrophages were undergoing apoptosis in the Ad-CMVpLpA or Ad-BAT treatments (fig. 2, top row).
|Fig. 2. Antisense to TGF-β1 promotes apoptosis of macrophages but myofibroblasts have little apoptotic activity. Magnification of representative 12-week images ×100, with insets ×400. Top row: cells expressing CD68 (macrophages) stain red and cells expressing active caspase 3 stain green. Colocalization of the 2 colors appears as yellow. Almost all of the CD68-positive cells in the Ad-AST treatment are undergoing apoptosis which contrasts with little apoptosis of CD68-positive cells in the other 2 treatments. Bottom row: cells expressing ASM-1 (myofibroblasts) stain red and cells expressing active caspase 3 stain green. There are no significant differences in the colocalization of the myofibroblast cell marker (ASM-1) and active caspase 3 between treatments. N = Neointima; A = adventitial/medial layer.
Cells expressing both ASM-1 and h-caldesmon are defined as mature smooth muscle cells, while cells expressing only ASM-1 are defined as myofibroblasts or synthetic muscle cells [25, 26]. In the Ad-AST treatment, there was a clear layer of mature smooth muscle cells in the adventitial/medial layer directly outside the internal elastic lamina, as shown previously [22]; however, these cells showed little colocalization with apoptosis or proliferation markers at any time point in any treatment.
Myofibroblasts are the dominant cell type in the neointima in all treatments; however, despite the number of these cells being significantly reduced in the Ad-AST treatment, there was no costaining with apoptosis markers (fig. 2, bottom row). No proliferation of myofibroblasts was seen in the Ad-CMVpLpA or Ad-BAT treatment either, indicating that Ad-AST must be affecting the transformation of fibroblasts into myofibroblasts or migration of myofibroblasts from outside the vein graft wall.
Apoptotic cells with fragmented DNA in the vein graft tissue sections were labeled via enzymatic reaction. This method was used to qualitatively determine that the significantly different levels of active caspase 3 activity previously described could be replicated when looking at a different step in the apoptotic process. DNA fragmentation assays showed the same trends of apoptotic activity between treatments as caspase, with fragmented DNA in the same locations and in the same relative amounts (fig. 3).
|Fig. 3. In situ detection of DNA fragmentation corresponds to active caspase 3 activity. Magnification of 4-week images representative of each treatment group ×400. The column on the left is active caspase 3 (green) with DAPI counterstain (blue). The column on the right is in situ detection of DNA fragmentation (red) and DAPI counterstain (blue). Top row: Ad-AST treatment; middle row: Ad-CMVpLpA treatment; bottom row: Ad-BAT treatment. N = Neointima; A = adventitial/medial layer.
The effects of TGF-β1 are partially realized by changes in proliferation and apoptosis of cells present in the vein tissue. The increase in TGF-β1 levels in a vein after the trauma of the grafting process is thought to be a component of the healing response in the vascular system [27]. While TGF-β1 is important for vessel healing, its continued presence in the tissue leads to IH formation and vascular disease [28].
TGF-β1 is a cytokine that has multiple effects on cell proliferation and death depending on its concentration [29] and the cell type that it is acting upon [30]. When the antisense TGF-β1 treatment is applied to the graft, we see that there are significantly more cells in the vessel at 1 and 2 weeks. This early increase in cell number is likely due to the Ad-AST treatment reducing TGF-β1 and therefore reducing the apoptosis of endothelial cells, increasing the release of paracrine factors by these cells to stimulate infiltration of cells from the bloodstream.
The antisense TGF-β1 treatment speeds the sequence of events involved in healing (whereas the treatment with bioactive TGF-β1 delays progressing through this sequence of events). For the Ad-AST treatment, the major increase in cell number occurred prior to the 1-week time point (the vein used for the graft has fewer cells per cross-section). Further observation shows that in the control (Ad-CMVpLpA), the largest fold increase in cell number occurred between 1 and 2 weeks, and in the Ad-BAT (overexpression of TGF-β1) treatment, this increase is delayed until between 2 and 4 weeks. However, after this initial delay, there are significantly more cells in the Ad-BAT and Ad-CMVpLpA treatments than in the Ad-AST treatment indicating that the cells that have now infiltrated these grafts are expressing the paracrine factors necessary to result in further cell infiltration and proliferation.
Trauma to an artery due to grafting causes TGF-β1 levels to increase dramatically [22]. The Ad-BAT and Ad-CMVpLpA treatments achieve high levels of TGF-β1 early, and this high level is maintained over time. The high level of TGF-β1 initially inhibits apoptosis of cell types that secrete extracellular matrix (myofibroblasts) which helps to heal the vein graft, but which eventually becomes responsible for IH [31]. The Ad-AST treatment keeps TGF-β1 levels low through the early stages of the healing response, reducing the infiltration and maturation of myofibroblasts.
In this study, we did not observe significant changes in cell proliferation, death or survival with Ad-BAT treatment. The rat epigastric vein utilized in our vein graft model is a relatively thin-walled vessel. In this respect, it is perhaps analogous to the human cephalic vein, which has a thinner medial layer than the human saphenous vein. Human cephalic vein grafts achieve long-term patency rates that are inferior to those of saphenous vein grafts with many graft failures resulting from marked IH. We believe our model is analogous to this extreme clinical situation with large amounts of TGF-β1 produced in the control grafted vein [21]. Given this high background of TGF-β1 production, additional TGF-β1 may fail to drive a significant change for many of our measures. For example, Ad-BAT treatment has minimal effect on IH in this model. However, there have been statistically significant increases in the mRNA for tissue inhibitor of metalloproteinase 1 and in the mRNA for collagen [22]. Interestingly, these effects were more pronounced at 1 and 2 weeks than at 4 and 12 weeks. With adenovirus vectors, protein expression at 2 weeks is characteristically 50% of that seen 4 days after transfection. Therefore, a diminishing treatment effect is expected for the Ad-BAT treatment with time.
This contrasts with the Ad-AST treatment which uses the same adenovirus vector. Significant differences in proliferation and apoptosis between treatments were not seen until 4 weeks after grafting. These differences between treatments were stronger still at 12 weeks. Thus, the early reduction in TGF-β1 levels is able to mediate changes that were propagated even after the antisense mRNA was no longer being expressed. We attribute this extended effect to an early resolution of chronic inflammation.
We have observed neutrophils infiltrating the cell wall, but macrophages are the major infiltrating cell type. At 1 week, macrophage can be more than 50% of the cells found in the vessel wall. Vein grafts treated with adenovirus designed to increase TGF-β1 have had approximately twice as many macrophages within the intima at 4 and 12 weeks as control veins. This treatment has also resulted in an additional smaller increase in macrophage within the media/adventitia. Treatment with Ad-AST did not change macrophage numbers in the neointima compared with controls. However, this treatment did reduce macrophage numbers in the media/adventitia to less than 50% of those seen in the controls at 4 and 12 weeks [22]. In these studies, we have shown that Ad-AST treatment leads to increased macrophage apoptosis. In all treatments, macrophages are present on the internal elastic lamina at 4 and 12 weeks. In the Ad-AST treatment, almost every macrophage on the internal elastic lamina is undergoing apoptosis while very few macrophages are undergoing apoptosis in the Ad-CMVpLpA and Ad-BAT treatments. Taking into consideration that macrophages migrate from the lumen to the adventitia by passing through the internal elastic lamina, the apoptosis of macrophage at the internal elastic lamina explains the previous observation of fewer macrophages reaching the media/adventitia.
After controlling for differences in cell numbers, the only significant difference in proliferation was in the Ad-AST treatment. At the 12-week time point, proliferation was increased by the Ad-AST treatment, but not as much as apoptosis was increased by this treatment. Proliferation markers did not colocalize exclusively with the cell type markers used in this study. However, we do observe an increase over time in the number of mature smooth muscle cells in the medial/adventitial layer of Ad-AST-treated vein grafts. This layer of smooth muscle cells may be proliferating between the 4- and 12-week time points when data were not collected, or smooth muscle cells may be migrating into the graft.
The Ad-AST treatment has been shown to reduce IH formation and cause the vessel to take on an artery-like phenotype. We have presented data that suggest these changes are at least partially mediated by causing the cell types responsible for the deposition of IH and other negative healing responses to undergo apoptosis (macrophages) or reducing their infiltration (myofibroblasts). In contrast, the Ad-BAT and Ad-CMVpLpA treatments showed little apoptosis of macrophages or myofibroblasts which allowed for unchecked extracellular matrix synthesis and the development of an extensive neointima.
This material is based on work supported by the Office of Research and Development, Biomedical Laboratory Research and Development Service, Department of Veterans Affairs. Glen Leverson, PhD, of the Department of Surgery Biostatistics Office assisted in the statistical analysis. Kristin Jakubowski, BS, provided valuable insight during the preparation of the manuscript. Ad-CMVpLpA was provided by the University of Michigan Medical Vector Core.
Dr. Randal Wolff
W.S. Middleton VA Hospital
2500 Overlook Terrace, G-wing Rm G9
Madison, WI 53705 (USA)
Tel. +1 608 265 9143, Fax +1 608 265 9144, E-Mail email@example.com
Received: August 10, 2007
Accepted after revision: December 4, 2007
Published online: March 20, 2008
Number of Print Pages : 10
Number of Figures : 3, Number of Tables : 1, Number of References : 31
Journal of Vascular Research (Incorporating 'International Journal of Microcirculation')
Vol. 45, No. 5, Year 2008 (Cover Date: August 2008)
Journal Editor: Pohl U. (Munich), Meininger G.A. (Columbia, Mo.)
ISSN: 1018–1172 (Print), eISSN: 1423–0135 (Online)
For additional information: http://www.karger.com/JVR
Background: The success of peripheral vein grafts is limited by intimal hyperplasia. Transforming growth factor (TGF)-β1 has effects on cell proliferation, apoptosis and extracellular matrix synthesis. We have previously observed positive changes in vessel healing with antisense to TGF-β1. Methods: Adenovirus was used to transduce rat femoral artery vein grafts with antisense to TGF-β1 (Ad-AST) or the sequence encoding the bioactive form of TGF-β1 (Ad-BAT). Grafts were harvested at 1, 2, 4 and 12 weeks and formalin fixed for immunohistochemical studies of the cell markers proliferating cellular nuclear antigen (proliferation) and active caspase 3 (apoptosis). In situ DNA fragmentation assays were also performed to confirm active caspase 3 results. Results: Ad-AST treatment significantly (p = 0.05) increased apoptosis of macrophages inside the internal elastic lamina. In addition, Ad-AST treatment significantly increased the cellularity of the graft at early time points and reduced it at later time points (p = 0.01). Conclusion: The low levels of TGF-β1 in Ad-AST treatment promote apoptosis of macrophages and provide an environment that is more conducive to the proliferation or infiltration of cells that contribute to healthy vessels.
© 2008 S. Karger AG, Basel
Dr. Randal Wolff
W.S. Middleton VA Hospital
2500 Overlook Terrace, G-wing Rm G9
Madison, WI 53705 (USA)
Tel. +1 608 265 9143, Fax +1 608 265 9144, E-Mail firstname.lastname@example.com
Received: August 10, 2007
Accepted after revision: December 4, 2007
Published online: March 20, 2008
Number of Print Pages : 10
Number of Figures : 3, Number of Tables : 1, Number of References : 31
Journal of Vascular Research (Incorporating 'International Journal of Microcirculation')
Vol. 45, No. 5, Year 2008 (Cover Date: August 2008)
Journal Editor: Pohl U. (Munich), Meininger G.A. (Columbia, Mo.)
ISSN: 1018–1172 (Print), eISSN: 1423–0135 (Online)
For additional information: http://www.karger.com/JVR
Received: 8/10/2007
Accepted: 12/4/2007
Published online: 3/20/2008
Issue release date: August 2008
Number of Print Pages: 10
Number of Figures: 3
Number of Tables: 1
ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)
For additional information: http://www.karger.com/JVR


text:
This Crysis 3 tutorial video demonstrates the features of the two gameplay modes available in the open beta: Hunter mode and Crash Site.
Crysis 3 multiplayer beta tutorial trailer
Crysis 3 360
- Developer
- Crytek Studios
- Publisher
- Electronic Arts
- Release Date
- 22 February 2013
- Genre
- Action: First-Person
- 3 Elite: Dangerous offline mode dropped, refunds offered
- New 3DS and Pokémon titles now available in NZ
- Steam's Fall Sale starts Nov. 27 – report
- 2 Valve tweaks Steam Early Access rules to protect players
- 2 Friday Briefing: Shadow of Mordor, GAME OF THE YEAR: 420BLAZEIT, and more
- 21 Grand Theft Auto V new-gen review
- 1 Isometric ARPG Shadows: Heretic Kingdoms out now
- Game of Thrones to feature HBO cast, first trailer released
- Destiny's The Dark Below opening cinematic leaks
- 2 Games with in-app purchases no longer listed as "Free" on iTunes
- Company of Heroes 2 standalone expansion Ardennes Assault out now
- Huge sandbox MMO Oort Online hits Steam Early Access
Don't miss a thing - get the day's top gaming stories blasted into your brain every evening.
Recently Popular
PlayStation TV available now
A non-portable Vita console.
Win a White PlayStation 4 with the Fricken Christmas Gamers Pack
Winner, winner, fricken dinner.
Far Cry 4 pirates out themselves over missing FOV slider
Only available in day one patch.
Artists of Draenor: realising a Warcraft expansion
We speak with Blizzard's Chris Robinson.
- 604 Eve Online
- 7240 Good deal thread
- 27 Past consoles that you hated
- 3671 What are you currently playing? (screenshots)
- 2485 Gaming General Discussion Thread
- 46 Do you (or would you) let your kid(s) play videogames?
- 85 Elite: Dangerous
- 20 Lost Ark - Korean ARPG
- 10 I Miss Battlefeild 1942 Desert Combat Final.
- 64 Smite


text:
Up Date Cobell Settlement 2014 When Will Checks Arrive In The Mail
When will i get my cobell settlement payment? : native, Why is it when we went to montana,we were told that our tribal i.d. numbers did not match the ones they have here in belcourt,north dakota. could it be that our. Cobell v. salazar indian trust settlement checks mailed!, Cobell v. salazar indian trust settlement checks mailed! by sarah pierce update: second cobell v. salazar settlement checks expected early 2014.. Cobell settlement payments - how do i apply? : native, The recent cobell settlement law will ultimately provide $3.4 billion in cash payments to native americans who have ownership rights in one or both of.
Deadline for second round of payments from cobell, (usa) -- the cobell settlement, approved on november 24, 2011, provides for a copy.9 billion trust land consolidation fund (fund). the settlement charges the u.s.. Cobell v. salazar class action website, Cobell v. salazar class action lawsuit back to top) 6. how do i demonstrate that i had an open iim account at any time between approximately 1985 to september 30. Indian trust settlement information, Information about the indian trust settlement (cobell) official indian trust settlement website questions and answers about the upcoming settlement.
Topic galleries - orlando sentinel - orlando news, weather, News, photos and information about orlando sentinel the brackets are set and the state's college basketball teams know their road maps to conference tournament. Techmeme, Blog tracking other technology blogs.. American kabuki: indian trust settlement payments released, The cobell settlement payments, being made by the indian trust settlement administrator, will be distributed in two payments with the first being the $.


text:
Holiday Gift Guide: Girly
Everyone has a friend or relative that's ultra-girly, so when Christmas rolls around you want to make sure you get them something that fits their feminine personality perfectly. Not sure what to get that girly-girl? Check out Kidzworld's Girly Holiday Gift Guide! Back to Article
← Previous 1 2 3 Next →


text:
Hamm makes a point at the Key Art Awards in Los Angeles. Plus: Gisele Bündchen, Katy & John, Vanessa Lachey and more
Credit: Fred Duval/FilmMagic
Published: Thursday Oct 18, 2012 | 05:00 PM EDT
More from PEOPLE
See More News
9 of 16
A well-suited Ben Affleck plays it cool during the premiere of his latest film, Argo, at the London Film Festival on Wednesday.
Copyright © 2014 Time Inc. All rights reserved. Reproduction in whole or in part without permission is prohibited.


text:
Vallejo bankrupt again? 'We are not going there'
The Vallejo city council last week voted to close a $5.2 million gap in the current budget, showing no alarm that in a five-year forecast the gap reopens, mainly driven by rising pension costs.
Moody's, a Wall Street credit rating agency, said in a report last month that Vallejo and two California cities currently in bankruptcy, Stockton and San Bernardino, risk returning to insolvency without pension relief.
Like this post? Chip in $5 to AFP.


text:
The Ramada Limited College Park offers Complimentary High Speed Wired and Wireless Internet and a Free Deluxe Continental Breakfast. The University of Maryland, Goddard NASA Space Center and the US Library of Agriculture/USDA Beltsville are all within 3 miles of this College Park hotel. This College Park Hotel offers easy access to Washington DC monuments, parks and attractions. This College Park / Washington DC area Hotel is adjacent to The University of Maryland and conveniently situated between BWI and Washington National Airports. Sports fans can enjoy events at the Washington Wizards MCI Center, the Washington Redskins FedEx Field and RFK Stadium all less then 10 miles from the Ramada Limited College Park. None of Washington DC / College Park, MD Hotels offer a more convenient location to The University of Maryland! This is the Best Location for Washington Redskins Football Games and all University of Maryland Terrapins sports events! College Park Hotels Top Choice for Location, Value and Service!
Welcome to the Ramada Limited College Park
24-hour front desk
Accessible facilities
Accessible parking
Air conditioning
Air conditioning
Alarm clock
Coffee/Tea maker
Complimentary continental breakfast
Complimentary in-room coffee or tea
Desk with lamp
Express check-out
Exterior corridors
Free newspaper
High speed internet access
High speed internet connection
High speed internet connection
Ice machine
Interior corridors
Internet services
Ironing board
Ironing board
Meeting rooms
Non-smoking rooms (generic)
Outdoor parking
Outdoor pool
Photocopy center
Remote control television
Rollaway bed
Vending machines
Wakeup service
Pizza Hut
Six Flags of America
RFK Stadium
Downtown Baltimore
US Air Arena
Washington DC Monuments
Baltimore Inner Harbor
University of Maryland
Camden Yards Orioles
Jack Kent Cooke Stadium
MCI Center
Showplace Arena
ARPT Washington National
ARPT Baltimore Washington
RENT Avis Rent a Car
Find Discount Hotels
Search Hotels by State
Group Hotel Rates
Our Best Destinations
Common Questions
Customer Testimonials
Contact SHDweb
Reserve Ramada Limited College Park
You are just a click or two away from the comforts of Ramada Limited College Park.Upon arriving at the hotel the concierge or other Ramada Limited College Park employees should have a wealth of local information. Ask for their suggestions and opinions about the College Park attractions you plan to visit.
We provide you with our most current hotel photos and property information plus lists of all the amenities and services provided at Ramada Limited College Park. Also for your convenience, we will give you detailed maps and directions to 9113 Baltimore Avenue, College Park as well as many other helpful details.Finding the right hotel when visiting College Park, Maryland should be as easy and simple as possible, and besides, hotel reservations should be the least of your worries. So, while you're visiting Maryland you concentrate on having fun and let us take care of shopping for the best hotel deals. It really is traveling simplified.
College Park Group DiscountsFind special group rates at the best hotels in College Park Maryland for your business meetings, sports team events, family reunions, or wedding party.Have fun & safe travels!
Follow @shdweb
Luxury HotelsSitemap© SHDweb.com


text:
Watch this issue
Current Issue
Like SURFING on Facebook
A huge package.
Full-length, full-love.
Seen behind the scenes.
Vans ECSC Final Day!
Vans and Captain Fin for a Stylish Combination
Off The Wall on the East Coast
A film of films.
Getcha kicks.
Vans does it again.
Working to live, or living to work?
The happiest men on earth.
Travel with the Gudang gang.
A fine wardrobe
Brought to you by Joel
A film premier for the ages
ATTN: San Diego
They say Virginia is for lovers
And ECSC is damn good proof
This fall, Vans is honored to embark on a second venture with Krochet Kids intl
Leila Hurst travels to the Lima to take the women surfing who handmake the fabrics used in the collection
World Tour tactics lead to success on the final day
Until 2014, Huntington Beach
This spring, Vans offers a colorful collection of functional boardshorts
Plenty of patterns to choose from
Dane presented his Vans Surf Sider Collab
John John presented "Done"
Hitting Stores in February
Shoe projects bring each band member's personal style and passions to life
Sign up for our newsletter and stay up-to-date with the latest industry news, featured stories, contests, events and much more. Your email will not be shared
with anyone. You may unsubscribe at any time. For more information on our privacy policy, please visit
Featured Flipbook:


text:
The 2015 tryout dates have been scheduled. Please save the dates for:
March 16th- 19th, 2015 5:30-9:30pm - Saints Sports Academy in Lakeville
Details regarding specific player times and format will be announced at a later time.
Visit FTBA on Facebook and Twitter
** Fields are open unless updated closed with the current date**
School District Field Hotline
City Field Line
651-280-6870 Option 5


text:
You must log in to access this feature. Sign in with Twellow or Facebook to get started.
By signing in to Twellow, you indicate your agreement to our terms of service.
Simply connect with Facebook and kiss those password blues away,
or go here to reset your password.
Long time Graphic Designer/Visual Artist who also does Interior Design and Cake Decorating. Anything visual is my speciality!