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Molecules 2014, 19(5), 5748-5760; doi:10.3390/molecules19055748
Published: 5 May 2014
Abstract: Inhibition of cytochrome P450 (CYP) and P-glycoprotein (P-gp) are regarded as the most frequent and clinically important pharmacokinetic causes among the various possible factors for drug-drug interactions. Scutellarin is a flavonoid which is widely used for the treatment of cardiovascular diseases. In this study, the in vitro inhibitory effects of scutellarin on six major human CYPs (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) and six rat CYPs (CYP1A2, CYP2C7, CYP2C11, CYP2C79, CYP2D4, and CYP3A2) activities were examined by using liquid chromatography-tandem mass spectrometry. Meanwhile, the inhibitory effects of scutellarin on P-gp activity were examined on a human metastatic malignant melanoma cell line WM-266-4 by calcein-AM fluorometry screening assay. Results demonstrated that scutellarin showed negligible inhibitory effects on the six major CYP isoenzymes in human/rat liver microsomes with almost all of the IC50 values exceeding 100 μM, whereas it showed values of 63.8 μM for CYP2C19 in human liver microsomes, and 63.1 and 85.6 μM for CYP2C7 and CYP2C79 in rat liver microsomes, respectively. Scutellarin also showed weak inhibitory effect on P-gp. In conclusion, this study demonstrates that scutellarin is unlikely to cause any clinically significant herb-drug interactions in humans when co-administered with substrates of the six CYPs (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) and P-gp.
1. Introduction
Pharmacokinetic drug-drug interactions (DDI) occur when a drug alters the absorption, distribution, metabolism, and excretion (ADME) of a co-administered drug, which may result in the increase or decrease of drug plasma concentration that can lead to serious adverse events or reduced drug efficacy. Cytochrome P450 (CYP), a superfamily of monooxygenases located primarily in hepatocytes, are the enzymes principally responsible for the metabolism of a lot of endogenous and exogenous compounds. It is generally accepted that 80%–90% of the clinical drugs are metabolized by CYP isoenzymes [1,2]. Of these, CYP3A4/5 accounts for approximately 30.2%, CYP2D6 for 20%, CYP2C8 for 4.7%, CYP2C9 for 12.8%, CYP2C19 for 6.8% and CYP1A2 for 8.9% [2]. P-glycoprotein (P-gp), a 170 kDa efflux glycoprotein encoded by the MDR-1 gene in humans belonging to the ABC superfamily, is localized in many tissues, including the apical surface of mature enterocytes, canalicular membranes of hepatocytes, and kidney and brain endothelial cells [3,4]. P-gp is responsible for the efflux of xenobiotics from cells and influences the pharmacokinetics of numerous drugs [5]. Alteration of CYP or P-gp activities involved in the absorption, distribution, metabolism, or excretion of a new compound by concomitant drugs maybe change drug exposure and affect drug response (safety or efficacy) [6].
Herbal medicines and their active ingredients are now widely used around the world and have become an important part of clinical medicine. In recent years, various studies have indicated that these phytochemicals have great potential to induce or inhibit the activities of CYP and/or P-gp, thus affecting the pharmacology and toxicology of the given drugs. A number of such herb-drug interactions (HDI) have been reported. For example, hyperoside is a potent selective CYP2D6 inhibitor in HLMs [7]. Baicalin can inhibit CYP2D and CYP3A activities in rat liver microsomes, and induce changes in the pharmacokinetics of midazolam and dextromethorphan in rats [8,9]. Rhinacanthin-C had an inhibitory effect on the expression and the function of P-gp, and the action was reversible [10]. Macelignan appeared to be effective to improve the cellular accumulation as well as oral exposure of paclitaxel, mainly via the inhibition of P-gp-mediated cellular efflux [11].
Scutellarin (Figure 1), a well-known flavone glycoside, is the primary active ingredient of an important Chinese traditional herbal medicine, Herba Erigerontis [Chinese name: Deng-zhan-xi-xin, Latin name: Erigeron breviscapus (Vant.) Hand.-Mazz.] belonging to the Compositae family that is mainly distributed in Yunnan Province of Southwest China and has been widely used as a folk remedy by the native people for the treatment of cardiovascular diseases and cerebrovascular diseases. Hence, many clinical preparations of Herba Erigerontis have been developed. Among these preparations, Herba Erigerontis injection (HEI), made from the aqueous extracts of Herba Erigerontis [12], is prominent for its remarkable curative effects for ischemic stroke, coronary heart disease and angina pectoris [13]. Our former study discovered that HEI can inhibit the activities of rat liver Cyp2d4 and Cyp3a2 in vivo [14]. Scutellarin is the capital effective component of HEI [12] and shows many significant beneficial pharmacological activities, such as antioxidant [15], anti-inflammatory [16,17,18], anti-diabetic complication [19], anti-hypertrophic [20], antiischemic [21,22], anti-obesity [23], anti-HIV-1 [24], anti-PRRSV [25], anti-tumor [26,27,28,29,30,31], hypercholesterolemia suppression [32], angiogenic [33], vasodilator [34], hepatoprotective [35] and neuroprotective effects [36,37,38,39,40,41,42,43]. Up to now, a lot of researches on the metabolism and pharmacokinetics of scutellarin in various models have been reported, but there is only one paper [44] that suggests that valsartan may inhibit the biliary excretion of scutellarin mediated by multidrug resistance-associated protein 2 and another paper [45] demonstrates that scutellarin, even at 100 mM, exerts weak inhibition towards the activity of UGT1A1, 1A6, 1A9 and 2B7 with 48.8%, 20.2%, 36.1%, and 73.8%, respectively. However, the studies of scutellarin concerning drug interactions based on CYPs and P-gp are rare. In addition, another result from acute and subacute toxicity studies suggests that scutellarin has a sufficient margin of safety for therapeutic use [46].
Consequently, we wanted to clarify whether scutellarin is responsible for the CYP inhibition activities of the HEI, even partly. At the same time, an increasing urgent demand of systematic investigation on HDI of scutellarin has been raised because of its wide applications. Therefore, the objective of this study was to systemically evaluate the in vitro inhibitory potential of scutellarin on the activities of six major cytochrome P450s (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) and a transporter (P-gp) with the aim of avoidance of pharmacokinetic HDI, which was worthy of promoting safety and efficacy of scutellarin in the clinic.
2. Results and Discussion
The substrates and inhibitors of CYPs and P-gp used in this study were in line with the FDA's guideline and previous reports [47,48,49,50]. These experimental methods have been validated in our previous study [51,52], and IC50 values of inhibitors were in good agreement with the published values according to the acceptable degree of accuracy [47,48,49,50].
Scutellarin was evaluated for the ability to inhibit the activities of the six CYPs (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) and P-gp. The IC50 values for six CYPs in human/rat liver microsomes are presented in Table 1. P-gp inhibition is shown in Figure 2.
|Table 1. The IC50 values of scutellarin on the activities of six major CYP isoenzymes in HLM and RLM.
|IC50 for HLM
|IC50 for RLM
CI: Confidence Intervals.
2.1. Inhibition by Scutellarin on Six CYPs in HLM
Using human liver microsomes and industry-accepted CYP substrates, scutellarin showed weak inhibitory effects on the six tested CYPs, as the IC50 values for CYP1A2, CYP2C8, CYP2C9, CYP2D6 and CYP3A4 were in excess of 100 μM, and only 63.8 μM for CYP2C19 (Figure 3).
2.2. Inhibition by Scutellarin on Six CYPs in RLM
Similarly, scutellarin had a weak inhibitory effect on six tested CYPs using rat liver microsome, the IC50 values for CYP1A2, CYP2C11, CYP2D4 and CYP3A2 were in excess of 100 μM, but 63.1 and 85.6 μM for CYP2C7 and CYP2C79 (Figure 4), respectively.
2.3. Inhibition by Scutellarin on P-glycoprotein
The effect of scutellarin on P-gp was examined by verapamil (100 μM) as the positive control after results showed that scutellarin, even at highest concentration, does not show cytotoxicity on WM-266-4 cells. All of the six concentrations examined (4.1~1,000 μM) for scutellarin produced less than 25% P-gp inhibition, which suggests that scutellarin cannot influence P-gp activity.
Our previous study discovered that HEI did not exert the inhibitory effects on rat liver CYP1A2, CYP2C11 and CYP2E1, but showed weak inhibitory activities on rat liver CYP2D4 and CYP3A2 in vivo [14]. Since scutellarin is the capital effective component of HEI [12] and it is necessary to investigate the in vitro inhibitory potential of scutellarin on CYP1A2, CYP2C7, CYP2C11, CYP2C79, CYP2D4, and CYP3A2 activities in RLM. These results showed that scutellarin (even at 100 μM) had no apparent inhibitory effects on rat CYP1A2, CYP2C11, CYP2D4 and CYP3A2 in vitro, but had weak inhibitory effects on rat CYP2C7 and CYP2C79 with IC50 values of 63.1 and 85.6 μM. Our present in vitro results were partly in accord with our former study, but our result on rat CYP1A2 was consistent with previous report that scutellarin was a poor inhibitor on rat CYP1A2 with an IC50 value of 108.20 ± 0.657 μM in in vitro experiments and exhibited a weak mixed-type inhibition against the activity of CYP1A2 with a Ki value of 95.2 μM, Scutellarin at 30 mg/kg also weakly inhibited CYP1A2 activity in whole animal studies [53]. Because there were only the results of rat in all above studies, we studied in vitro inhibitory effects of scutellarin on human CYP1A2, CYP2C8, CYP2C9, CYP2D6 and CYP3A4. The in vitro results showed that scutellarin (even 100 μM) had no apparent inhibitory effects on human CYP1A2, CYP2C8, CYP2C9, CYP2D6 and CYP3A4 activities, but had weak inhibitory effects on human CYP2C19 activity with IC50 values of 63.8 μM. In our studies, there are no significant difference between the IC50 values of scutellarin on human/rat CYP2C8 and CYP2C19 which may be accepted for species differences. A compound that inhibits an isoform with IC50 value of less than 10 μM is generally considered as a "potent" inhibitor to the isozyme, whereas the compound with IC50 value between 10 and 50 μM is a "moderate" inhibitor [54,55]. Results from the in vitro studies can be used to predict in vivo interaction and guide the need for further in vivo study evaluation though it is not possible to directly extrapolate the in vitro results with what can occur in vivo. These viewpoints are widely accepted by researcher on CYP inhibition or drug interactions. So, we believed that scutellarin may have no significant inhibitory effects on CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP3A4.
The WM-266-4 is an atypical cell line used in studies about drug interactions. Therefore, the method on P-gp inhibition-calceinAM assay was carefully validated in HD Biosciences Co., Ltd. and accordant with FDA's guideline. Furthermore, we found that scutellarin (even at the concentration of 1 mM) had no significant inhibitory effects on P-gp in WM-266-4 cells in this study.
3. Experimental
3.1. Materials
Scutellarin (LOT 110842-200605) was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Phenacetin, diclofenac, midazolam, dextromethorphan, acetaminophen, furafylline, quinidine, 1-hydroxymidazolam, dextrorphan, sulfaphenazole, gemfibrozil, buspirone, calcein-AM, and verapamil were purchased from Sigma (St. Louis, MO, USA). Paclitaxel, S-mephenytoin, 4-hydroxymephenytoin, S-(+)-N-benzylnirvanol, montelukast, and ketoconazole were purchased from TRC (Toronto, ON, Canada). 6α-Hydroxypaclitaxel was purchased from Calbiochem (San Diego, CA, USA). 4-hydroxydiclofenac was purchased from BD Gentest Corporation (Woburn, MA, USA).
Pooled human liver microsomes (HLM, LOT 32556) were purchased from BD Gentest Corporation (simple donor information see Table 2.). Pooled rat liver microsomes were prepared from three male Sprague-Dawley rats in HD Biosciences Co. (Shanghai, China). The microsomal preparations contain a protein concentration of 10 mg/mL and are stored in a −80 °C freezer before use. The human metastatic malignant melanoma cell line WM-266-4 was procured from ATCC (Manassas, VA, USA). Glucose-6-phosphate (G-6-P) and NADP+ were purchased from Majorbio Co. (Shanghai, China). Glucose-6-phosphate dehydrogenase (G-6-PDH) was purchased from Calbiochem (Gibbstown, NJ, USA). The assay kit for protein determination with a biscinchoninic acid reagent was from Pierce (Rockford, IL, USA). Water was purified using a Milli-Q system (Millipore, Bedford, MA, USA) and used throughout the study. All inorganic salts were of analytical grade and were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). All organic solvents were of high performance liquid chromatography (HPLC) grade and were purchased from Sigma-Aldrich, USA.
|Table 2. Simple information on fifty donors (Catalog No. 452156).
|Age (From 21 to 77 Years)
|African Ameracian
3.2. Instrument
A Shimadzu LC-20A liquid chromatographic system equipped with a DGL-20A vacuum degasser, a dual pump, and a SIL-20A autosampler (Shimadzu, Tokyo, Japan) was used. Detection was performed using an API 4000 mass spectrometer equipped with a TurboIonSpray (ESI) Interface (Applied Biosystems, Concord, ON, Canada). The Analyst 1.5 software package (Applied Biosystems, Concord, ON, Canada) was used to control the LC-MS/MS system, and for data acquisition and processing. Fluorescence was measured by Flexstation II 96 Scanning Fluorometer (Molecular Devices, Sunnyvale, CA, USA).
3.3. CYP450 Assay
3.3.1. Microsomal Incubations and Treatment Protocol
Incubation mixtures were prepared in a total volume of 60 μL with final component concentrations as follows: 0.1 M potassium phosphate buffer (pH 7.4), 1.0 mM NADP+, 4.0 mM MgCl2, 10 mM G-6-P, 1 U/mL G-6-PDH, 0.3 mg/mL human liver microsome or 0.5 mg/mL rat liver microsome, and Scutellarin (serial concentrations of 100, 30, 10, 3.0, 1.0, 0.3, 0.1, and 0.03 μM) in triplicate. Positive controls (furafyline for CYP1A2, montelukast for CYP2C8, sulfaphenazole for CYP2C9, S-(+)-N-3-benzylnirvanol for CYP2C19, quinidine for CYP2D6, ketoconazole for CYP3A4) and specific substrates (phenacetin for CYP1A2, paclitaxel for CYP2C8, diclofenac for CYP2C9, S-mephenytoin for CYP2C19, dextromethorphan for CYP2D6, midazolam for CYP3A4) are consistent with our previous papers [48,49]. The substrates were used at concentrations approximately equal to their respective Michaelis-Menten constant (Km) values: 10 μM phenacetin, 5 μM paclitaxel, 5 μM diclofenac, 200 μM S-mephenytoin, 5 μM dextromethorphan, and 2 μM midazolam. NADP+ was added after a 15 min preincubation of all other components at 37 °C. After a given incubation time (30 min), the reactions were terminated by addition of 60 μL ice-cold acetonitrile containing gemfibrozil and buspirone as internal standards. Incubation samples were stored at −80 °C until used in liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The mixture was centrifuged at 3,500 rpm for 5 min. The supernatant was mixed with equal volume of a 1:1 mixture of methanol and water and 20 μL of the final mixture was used for LC-MS/ MS analysis.
3.3.2. LC-MS/MS Analysis
Chromatographic separations were performed using the Waters Nova-Pak® C18 (150 × 3.9 mm) column. The column was maintained at ambient temperature (25 °C). A post-column diverter valve was used to direct the HPLC column eluate to a waste container for the first 3.2 min of the chromatographic run and then to the ionization source. The flow rate was maintained at 0.7 mL/min and the mobile phase was used as follows: solvent A [0.1% formic acid in mixture of acetonitrile/methanol/formic acid (50/50/0.1)] and solvent B (5 mM NH4Ac).
The HPLC gradient program used was as follows: (1) mobile phase B was set to 5% at 0 min; (2) a linear gradient was run to 15% B in 2.0 min; (3) a linear gradient was run to 80% B in 3.3 min; (4) a linear gradient was run to 90% B in 3.6 min; (5) a isocratic gradient was run to 90% B in 4.0 min; (6) a linear gradient was run to 15% B in 7.0 min; and (7) the solvent composition was returned to 5% B in 0.1 min for re-equilibration for 2 min. The mass transitions of the metabolites of specific substrates using API4000 LC-MS/MS were consistent with our previous papers [48,49].
3.4. P-glycoprotein Assay [49]
One vial of WM-266-4 cells was thawed and cultured in a 15 cm culture dish overnight. Cells were split the next day and transferred onto a clear bottom 96-well plate with 5.0 × 104 cells per well. Scutellarin was serially diluted to concentrations of 2000, 666.7, 222.2, 74.1, 24.7, and 8.2 μM with a dilution factor of 3, using culture media without serum. A volume of 50 μL of each serial dilution of scutellarin was added to the 96-well plate in triplicate. The final concentrations of scutellarin in this reaction were 1000, 333.4, 111.1, 37.1, 12.4, and 4.1 μM. Verapamil was used as a positive control at a concentration of 100 μM and 1% DMSO was used as a negative control. After 30 min of incubation time, calcein-AM diluted with 50 μL culture media without serum was added to a concentration of 1 μM and the sample was incubated for an additional 30 min. The plates were placed in the Flexstation II (Molecular Devices) and fluorescence was measured at excitation and emission wavelengths of 494 and 517 nm, respectively.
3.5. Data Analysis
The CYP inhibition potential were evaluated by measuring the formation of one or more metabolites of the test substrates. The peak area ratios of the metabolites and internal standard were acquired using the Analyst 1.5 software package (Applied Biosystems). The peak area ratios were plotted as a percentage of the relevant negative control for each reaction and the 50% inhibitory concentration (IC50) values were calculated by nonlinear regression using Graphpad Prism 5.0 (San Diego, CA, USA).
The P-gp inhibitor verapamil was used as positive control at a concentration of 100 μM. The experiment was considered valid if an increase in fluorescence greater than 5-fold was observed. For P-gp inhibition, a compound with a response less than 25% of verapamil at 100 μM is normally reported as "negative" at the tested concentration. The percent response value was used to determine and report the P-gp interaction at the tested concentration. A response equal to or greater than 25% of verapamil at 100 μM was considered to be "positive" at the tested concentration.
4. Conclusions
In conclusion, the present study demonstrated that scutellarin showed no significant inhibitory effects on CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP3A4 and P-gp, and it could not be the compound or a member of Herba Erigerontis injection which can inhibit CYP activities. Scutellarin is safe and unlikely to cause any clinically significant herb-drug interactions and thus cause the occurrence of adverse drug reactions in humans when co-adminstrated with subtrates of the six CYPs and P-gp.
This study was supported by the Traditional Chinese Medicine Scientific Research Fund of the Shanghai Municipal Health Bureau (No 2012L058A). We thank Xiao-yan Li and Gui-xuan Chai from HD Biosciences Co., Ltd. for their technical assistance.
Author Contributions
Guo and Han conceived the project and wrote the first draft. All authors contributed to data analysis and interpretations and to the writing of subsequent drafts. All authors have seen and approved the final version of the manuscript. All authors read and approved the final manuscript.
Conflicts of Interest
The authors declare no conflict of interest.
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- Chai, L.; Guo, H.; Li, H.; Wang, S.; Wang, Y.L.; Shi, F.; Hu, L.M.; Liu, Y.; Adah, D. Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes. J. Ethnopharmacol. 2013, 150, 100–107, doi:10.1016/j.jep.2013.08.011.
- Guo, H.; Hu, L.M.; Wang, S.X.; Wang, Y.L.; Shi, F.; Li, H.; Liu, Y.; Kang, L.Y.; Gao, X.M. Neuroprotective effects of scutellarin against hypoxic-ischemic-induced cerebral injury via augmentation of antioxidant defense capacity. Chin. J. Physiol. 2011, 54, 399–405. 22229507
- Guo, L.L.; Guan, Z.Z.; Wang, Y.L. Scutellarin protects against Aβ-induced learning and memory deficits in rats: Involvement of nicotinic acetylcholine receptors and cholinesterase. Acta Pharm. Sin. 2011, 32, 1446–1453, doi:10.1038/aps.2011.115.
- Guo, L.L.; Guan, Z.Z.; Huang, Y.; Wang, Y.L.; Shi, J.S. The neurotoxicity of β-amyloid peptide toward rat brain is associated with enhanced oxidative stress, inflammation and apoptosis, all of which can be attenuated by scutellarin. Exp. Toxicol. Pathol. 2013, 65, 579–584, doi:10.1016/j.etp.2012.05.003.
- Li, J.H.; Lu, J.; Zhang, H. Functional recovery after scutellarin treatment in transient CEREBRAL ischemic rats: A pilot study with 18 F-fluorodeoxyglucose micropet. Evid. Based Complement. Alternat. Med. 2013, 2013, doi:10.1155/2013/507091.
- Xu, W.; Zha, R.P.; Wang, W.Y.; Wang, Y.P. Effects of scutellarin on PKCgamma in PC12 cell injury induced by oxygen and glucose deprivation. Acta Pharm. Sin. 2007, 28, 1573–1579, doi:10.1111/j.1745-7254.2007.00502.x.
- Zhu, J.; Choi, R.; Li, J.; Xie, H.; Bi, C.; Cheung, A.; Dong, T.; Jiang, Z.; Chen, J.; Tsim, K. Estrogenic and neuroprotective properties of scutellarin from Erigeron breviscapus: A drug against postmenopausal symptoms and Alzheimer's disease. Planta Med. 2009, 75, 1489–1493, doi:10.1055/s-0029-1185776.
- Wang, S.; Wang, H.; Guo, H.; Kang, L.; Gao, X.; Hu, L. Neuroprotection of scutellarin is mediated by inhibition of microglial inflammatory activation. Neuroscience 2011, 185, 150–160, doi:10.1016/j.neuroscience.2011.04.005.
- Cui, M.Y.; Tian, C.C.; Ju, A.X.; Zhang, C.T.; Li, Q.H. Pharmacokinetic interaction between scutellarin and valsartan in rats. Acta Pharm. Sin. 2013, 48, 541–546.
- Ma, G.Y.; Cao, Y.F.; Hu, C.M.; Fang, Z.Z.; Sun, X.Y.; Hong, M.; Zhu, Z.T. Comparison of Inhibition Capability of Scutellarein and Scutellarin Towards Important Liver UDP-Glucuronosyltransferase (UGT) Isoforms. Phytother. Res. 2014, 28, 382–386, doi:10.1002/ptr.4990.
- Li, X.; Wang, L.; Li, Y.; Bai, L.; Xue, M. Acute and subacute toxicological evaluation of scutellarin in rodents. Regul. Toxicol. Pharm. 2011, 60, 106–111, doi:10.1016/j.yrtph.2011.02.013.
- Guidance for Industry: Drug interaction studies-study design, data analysis, and implications for dosing and labelling recommendations.
- Pelkonen, O.; Turpeinen, M.; Hakkola, J.; Honkakoski, P.; Hukkanen, J.; Raunio, H. Inhibition and induction of human cytochrome P450 enzymes: Current status. Arch. Toxicol. 2008, 82, 667–715, doi:10.1007/s00204-008-0332-8.
- Walsky, R.L.; Obach, R.S. Verification of the selectivity of (+)N-3-benzylnirvanol as a CYP2C19 inhibitor. Drug Metab. Dispos. 2003, 31, 343, doi:10.1124/dmd.31.3.343.
- Yuan, R.; Madani, S.; Wei, X.X.; Reynolds, K.; Huang, S.M. Evaluation of cytochrome P450 probe substrates commonly used by the pharmaceutical industry to study in vitro drug interactions. Drug Metab. Dispos. 2002, 30, 1311–1319, doi:10.1124/dmd.30.12.1311.
- Han, Y.L.; Yu, H.L.; Meng, X.L.; Li, D.; Zhou, Z.Y.; Yu, Q.; Wang, F.J.; Zhang, X.Y.; Guo, C. In vitro inhibition of Huanglian [Rhizoma coptidis (L.)] and its six active alkaloids on six cytochrome P450 isoforms in human liver microsomes. Phytother. Res. 2011, 25, 1660–1665, doi:10.1002/ptr.3475.
- Han, Y.L.; Yu, H.L.; Meng, X.L.; Li, D.; Zhou, Z.Y.; Yu, Q.; Wang, F.J.; Zhang, X.Y.; Guo, C. Inhibitory effects of limonin on six human cytochrome P450 enzymes and P-glycoprotein in vitro. Toxicol. In Vitro 2011, 25, 1828–1833, doi:10.1016/j.tiv.2011.09.023.
- Jian, T.Y.; He, J.C.; He, G.H.; Feng, E.F.; Li, H.L.; Bai, M.; Xu, G.L. Scutellarin inhibits cytochrome P450 isoenzyme 1A2 (CYP1A2) in rats. Phytother. Res. 2013, 26, 1226–1230.
- Dierks, E.A.; Stams, K.R.; Lim, H.K.; Cornelius, G.; Zhang, H.; Ball, S.E. A method for the simultaneous evaluation of the activities of seven major human drug-metabolizing cytochrome P450s using an in vitro cocktail of probe substrates and fast gradient liquid chromatography tandem mass spectrometry. Drug Metab. Dispos. 2001, 29, 23–29. 11124225
- Herzke, D.; Thiel, R.; Rotard, W.D.; Neubert, D. Kinetics and organotropy of some polyfluorinated dibenzo-p-dioxins and dibenzofurans (PFDD/PFDF) in rats. Life Sci. 2002, 71, 1475–1486, doi:10.1016/S0024-3205(02)01924-0.
- Sample Availability: Samples of the compounds and materials are available from the authors.
© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).


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Results 31 - 40 of 64 matches
Reading Time Series Plots
Shelley Olds, UNAVCO
This activity provides a brief introduction to GPS and provides a student activity to practice creating and reading time series plots with simplified GPS data and with authentic GPS data from Iceland.
Earthquake Investigation Workshop: Shake, Rattle, & Rock
Daniel Murray, University of Rhode Island
This workshop is part of an NSF-funded effort to provide professional development to STEM teachers in Rhode Island. In this activity, students will make "earthquakes" using a simple model, the earthquake ...
Cascadia Great Earthquake and Tsunami Suite
Michael Mayhew, National Science Foundation;
Michael Mayhew and Michelle Hall, Science Education Solutions Summary The Cascadia Earthquakes and Tsunami Suite contains five case studies organized around understanding the potential for large earthquakes and ...
Earthquake Hazards: The next big one?
John Taber, IRIS Consortium
Students work in small groups or individually to investigate the earthquake hazards in California, Missouri, and their own location. Students begin the activity with an exploration of the concept of probability, ...
CCD & Calcareous Ooze Assignment
Cindy Lampe, Ventura College
The assignment pre-tests student understanding of the CCD, lysocline, calcareous ooze, and the deposition of marine sediments near mid-ocean ridges and ocean basins.
What Can (and Cannot) Be Learned from Scientific Drilling Using Examples from Margins Initiatives
Rosemary Hickey-Vargas, Florida International University
This is a lecture segment that could be introduced to an introductory geoscience class to help explain and demonstrate what can and cannot be found out by scientific drilling.
Density, Isostasy, and Topography
Anne Egger, Central Washington University
Two students measure the density of different rock types by first measuring water displacement of the irregular pieces. Details In this activity, students develop an explanation for the bimodal topography of the ...
HMK 1_Plate Boundaries: Present, future, & past
Brian Hampton, Michigan State University;
This project builds on in-class exercises and lectures by having students learn to identify modern, future, and past plate boundaries. Students will use trends in topography, distribution of earth quake and ...
Northridge: A Case Study of an Urban Earthquake
Michael Mayhew, National Science Foundation;
Michael Mayhew and Michelle Hall, Science Education Solutions Summary The 1994 Northridge Earthquake Case Study explores the mystery of how such a major fault could have been missed within a tectonic basin that is ...
Layers of the Earth
Steven Semken, Arizona State University at the Tempe Campus; Tracy Perkins, South Mountain Community College
Students use graphs of seismic wave travel times, and value for the diameter of Earth obtained in the Size of the Earth activity, to investigate the internal structure of the Earth and determine that it is layered. ...

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Hazelnut Chiffon Cake
The hazelnuts give this cake a surprising crunch. It's covered with a buttery mocha frosting. —Joylyn Trickel
12-16 Servings
Prep: 1 hour Bake: 1 hour + cooling
2-1/4 cups cake flour
1-1/2 cups sugar
1 cup finely chopped hazelnuts, toasted
1 teaspoon baking powder
1/2 teaspoon salt
5 egg yolks
2/3 cup water
1/2 cup canola oil
1 tablespoon
McCormick® Pure Vanilla Extract
8 egg whites
1-1/2 teaspoons cream of tartar
3/4 cup butter, softened
1-1/2 cups confectioners' sugar
2 tablespoons baking cocoa
1 teaspoon
McCormick® Pure Vanilla Extract
2 to 3 tablespoons hot brewed coffee
In a large bowl, combine the flour, sugar, hazelnuts, baking powder
and salt. Whisk the egg yolks, water, oil and vanilla; add to dry
ingredients. Beat until well blended. In another large bowl, beat
egg whites and cream of tartar on medium speed until soft peaks
form; fold into batter.
© Taste of Home 2014
2 of 2
Hazelnut Chiffon Cake
Directions (continued)
Gently spoon into an ungreased 10-in. tube pan. Cut through the
batter with a knife to remove air pockets. Bake on the lowest rack
at 325° for 60-70 minutes or until top springs back when lightly
touched. Immediately invert the pan onto a wire rack; cool
completely. Carefully run a knife around edges and center tube to
loosen; remove cake.
In a small bowl, cream butter and confectioners' sugar. Beat in the
baking cocoa, vanilla and enough coffee to achieve desired
consistency. Frost cake. Yield: 12-16 servings.
© Taste of Home 2014


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Details: Free First Class / Priority Mail Shipping to United States on All Items.
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Save an average of $6 with 2 coupon codes & deals for jennyskincare.com. Jenny Skin Care is an online store that sells a wide variety of cosmetics. Some of the featured items include body lotions, creams, masks, tea, body wash, eye care, scrubs, treatments, cleansers and tonics, kits, serums, and more.
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Top 10 Series 5 Moments
We've compiled a video of our ten favourite moments from Series Five. It's was a tough job as there were so many great ones to choose from, but we got there in the end! Enjoy the video and feel free to list your own favourite moments in the comments.
Click show to see the list show
Step back in time...
COMMENT GUIDELINESPlease be civil and keep article comments relevant and on topic. Flag and report any offensive/trolling behavior, or contact us with details.
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Yesterday we hosted our first workshop on hacking sex toys and we had a wonderful and diverse turnout. The workshop started with people talking about ideas they had about toys to make. People were encouraged to use the parts available, bike tires to make harnesses, plastic eggs with tiny motors to make vibrators, or take […]
Help Us Do More Cool StuffAll donations are tax-deductible.
What Is The Hacktory?
Hack (def): to find a new (sometimes inelegant) solution to a problem
Factory (def): a place where products are made, or art like Andy Warhol's Factory
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I can't speak for you, but here are five things coming to small screens that I don't want to miss.
Sharks. Tornados. Sharknados. If you thought Sharknado was worthy of Mystery Science Theater 3000 treatment after the intentionally crappy flick premiered on Syfy on July 11, 2013 (and somehow went on to become a worldwide sensation), you are not only in luck but you get to see live Sharknado skewe ... More >>
[Mental Notes] Rebecca Black is this year's Antoine Dodson
Rebecca Black "Friday" Ark Music FactoryDon't give us that look--who could be better for a Beat Blvd. entry? She's totally local (from Anaheim Hills), she's got a new release, and let's face it, if everyone isn't actually talking about her, the fact that you're reading this via the Net means th ... More >>


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Secrets of Success< Secrets of Success Main Page
All Secrets submitted by SUNSHINE6442
Please note these are member-submitted secrets and are not reviewed by SparkPeople's health experts for accuracy. If you feel a secret is inappropriate, please click the "Report Inappropriate Secret" on the secret's page and our staff will review it.
Reduce Cholesterol With A Sweet Potato Burger
Walnuts have omega 3 fatty acids that protect the heart.....3 tbsp. daily helps reduce bad cholesterol. Mix small pieces into burgers,or to coat chicken or fish.
If you sprinkle cinnamon on your oatmeal in can metabolize sugar 20 times better as reported by Prevention Magazine.
- Submitted by SUNSHINE6442 5/17/2014 in Living With Diabetes | 1 Comment
Prevention Magazine reports if you drink from a Plastic Bottle or microwave food with plastic wrap chemicals may be leaching into you. Plastic contains a hormone disrupter and the higher levels of BPA ....the greater chance a person is to be obese. They also suggest flaxseeds to help remove toxins.
Prevention reports Our bodies are not designed to filter out chemicals and they usually get stored as fat. High Fructose corn syrup can quickly convert to fat in our systems. They also say artificial chemical can interfere with the brain and will throw metabolism out of balance.
- Submitted by SUNSHINE6442 5/17/2014 in Living With Diabetes | 1 Comment
Prevention reports that cayenne can speed up metabolism and lower blood sugar level before excess insulin can store fat. Don't forget to eat a small snack every 2-3 hours to help stable blood sugar levels!
Pain is always associated with inflammation
- Submitted by SUNSHINE6442 5/15/2014 in Living With Diabetes | 0 Comments
Skinny Meatloaf..Use Oatmeal Instead Of Bread Crumbs
Skinny Meatloaf
- Submitted by SUNSHINE6442 5/15/2014 in Living With Diabetes | 2 Comments
Try a cinnamon stick in your coffee or a sprinkle of powdered cinnamon. Cinnamon Helps Type 2 Diabetes .....Also Helps Cholesterol....cinnamon, cloves, bay leaves, and turmeric have all shown promise in enhancing insulin's action.
- Submitted by SUNSHINE6442 5/15/2014 in Living With Diabetes | 1 Comment
Keep our blood sugar level in better balance
Apples...Eating AN APPLE A DAY REDUCES CHOLESTEROL LEVELS THAT STOP ARTERY CLOGGING STICKINESS...as reported by Ohio State university Robert DiSilvestro PH.D.
- Submitted by SUNSHINE6442 4/29/2014 in Living With Diabetes | 0 Comments
Strawberries with Balsamic Vinegar
Strawberries with a high quality balsamic vinegar....splash them.Strawberries are good for diabetics, as they help stabilize the level of blood glucose been associated with inhibiting the production of cholesterol in the liver...and reducing heart disease...vinegar may mimic the effects of both Acrabose and Metformin which are two of the commonly prescribed medications for diabetics says Carol Johnston, Ph.D., As reported by WebMD
- Submitted by SUNSHINE6442 4/24/2014 in Living With Diabetes | 1 Comment


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Best Bars For Billiards In Detroit
One of the great things about Detroit is having a nice mix of older and newer establishments that offer billiards.
Best Tapas in Metro Detroit
Picky eaters love tapas. Eat a small amount of a variety of foods without spending a fortune at Centaur, Dylan's Raw Bar and Grill, Sangria and more.


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Legislación y Documentos Elaborados por OSCE
Convenios - Noticias
Informes y Reportes sobre Contratación Pública
Estudios sobre Contratación Pública
Otras Publicaciones
VI Conferencia Anual de Compras Gubernamentales 2010
OSCERVANDO - Revista Institucional
Notas de Prensa
Información de Interés y Procesos
Banners - Accesos Directos
Centro de Consultas del OSCE
Silencio Administrativo
has taken a path of sustained high economic growth, while it is getting better welfare conditions for its inhabitants. Both processes require a highly efficient public procurement, to facilitate the investment needed for growth and timely delivery of goods, civil works and services required by the community. Peruvian society requires, with full reason, that that system is also transparent and free of corruption.
The country is also engaged in a process of political and administrative decentralization to bring the State to achieve the community and return to it the ability to control. One of the conditions for successful decentralization is to have tools that facilitate regional and local government procurement to help them fulfill their mission effectively, with minimal risk of corruption and yet can be totally and permanently monitored by citizens.
The modernization of public procurement in the raised guidance management, requires a guide for a thorough reform of the entire system. Therefore, the effective date of Supervising Agency of the Government Procurement (OSCE) marks the starting point of this transformation, which also involves a change-oriented new public management approach that promotes the state, which is none other than management for results.
The new Act and Regulations, which came into effect from February 1
, are designed to put the OSCE in the service of public institutions and its citizens, this means a daily task and define our actions: a "Timely Service
on Public Procurement".
Central de Consultas * 614-3636 - Horario de Atención: 8:30 a 17:30 hrs. / Central Administrativa * 613-5555 - Horario de Atención: 8:30 a 17:30 hrs.
Trámite Documentario * Horario de Atención: 8:30 a 16:30 hrs. / Central Administrativa: Av. Gregorio Escobedo cdra. 7 s/n Jesús María - Lima 11/Perú
Trámite Documentario: Av. Punta del Este s/n Edificio El Regidor 1er. piso Nº 108 zona comercial del Conjunto Residencial San Felipe-Jesús María-Lima 11/ Perú


text:
Transform data center economics and meet dynamic business demands
Click here to download now
Overview: This paper is designed to help you understand the business case for HP BladeSystem Matrix, a converged infrastructure platform for delivering shared services.
HP BladeSystem Matrix delivers one virtualized pool of network, storage, and compute resources that can be continuously optimized and instantly adjusted to meet dynamic business demands for any workload type. It unites the tools, processes, and architecture of your physical and virtual worlds to help you cut costs and speed time to service delivery. .


text:
Basic in design, this classically styled barstool from Armen cuts a nice profile. It affords a 360 degree swivel and a well-padded seat for superior comfort. Rich brown chocolate leather covers the seat for a visually pleasing look. Legs and frame are manufactured in sturdy, bold espresso for a smart all around appearance.
Product Specs:
- 360 degree swivel
- Backless
- Rich brown chocolate leather
- Espresso legs
Finish: Brown Leather / Espresso Wood
26"H x 17.5"Round
Furnished Over 1 Million Homes Since 1997...
I was very satisfied with One Way Furniture Service. I have referred them to many friends, family, and customers. Thank you. Kathy A. Eisele, Boston, MA
Product Review - Armen 26" Backless Swivel Barstool Brown
Product Reviews:1 user(s) rated out of 5 stars
Now everyone can hang out & watch mom cook! - T2
Furniture Brand
Combining a healthy dose of retro, Armen Living Contemporary Furniture has stylish yet functional pieces for relaxing or entertaining. Whether you're looking for bar stools, dining tables and dining chairs, occasional tables, sofas, sectionals, or chaises, club chairs, accent chairs, ottomans or benches, Armen Living Contemporary Furniture is the perfect choice - the height of chic, and the finest in furniture craftsmanship, fabric, and materials. One Way Furniture offers high quality living room, dining room, and home bar furniture at discount prices. All living room, dining room and home bar furniture ships free. Armen Living
Weight & Size
Reference - Sku: LCMBS404BAES26 - Armen 26" Backless Swivel Barstool Brown by Armen Living


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Let's Have Brunch Party Invitation
Get a group of friends together for brunch with this whimsical invitation. Nifty graphic images spell out all the food fun to be had.
Size - A7 flat (5" x 7")
*Offer valid for new email subscribers. Promo code will be delivered via email.


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I have to wonder how testing could possibly have missed this. It would seem like a very basic test of this code would have included sending an invalid code (or a code from an invalid party). And checking to see that it fails properly.
I am aware that thorough testing is one of the parts of code development most likely to get skimped when deadlines loom. But managing to miss this in testing is really impressive.
Current best practice is to implement "continuous integration" so that tests and checks are run on every committed change to the code. This prevents tests not being run due to time constraints. However, I'm not sure whether they would help here: that assumes that test case has been defined and the test code written. This is a non-trivial case and the sort that you write after this kind of bug crops up, or the penetration testing guys think up.
In this case static code analysis or compiler flags should have picked this up. The code analysis should be part of the existing continuous integration process. If it was, then maybe it needs beefing up or replacing. If it wasn't, then hopefully it will be next time.
Embarrassing as it is, it's still good that Apple has admitted to quite how stupid the error was. Can't think of a better way of alerting developers to this kind of problem. Maybe they'll also finally get round to playing better with the slew of open source libraries they use.
Still waiting for the fix here but fortunately, MacOS is not on wifi (avoid it when you can) and I don't use many Apple products. I do wonder how Apple will manage to safely distribute the fix via the presumably compromised ITunes!


text:
The Taste Makers
These days, well-dressed can describe both what you make out of a closet and inside a kitchen. As foodie-ism has come into fashion, the fashionable set has flocked to the world of food. Meet eight women making their way at the intersection of sustenance and style.
By Heather John
Photo Credit: Courtesy of David Schulze
KERRY DIAMOND, 44 and CLAUDIA WU, 37
The Editors
WHAT THEY DO: Diamond is editorial director of Cherry Bombe, a biannual food and style glossy, and co-owner of Brooklyn restaurants Seersucker and Nightingale 9 and coffee shop Smith Canteen. Wu, a graphic designer, is creative director of Cherry Bombe.
DAILY UNIFORM: Diamond favors Chance striped T-shirts, Zero + Maria Cornejo dresses, and Me&Ro jewelry. Wu prefers dresses by Thakoon Addition and 3.1 Phillip Lim and cashmere from J.Crew.
FASHION CRED: Diamond was a public relations executive at Lancôme and Coach, and Wu launched the cult indie magazine Me and freelance art-directed for Prada Beauty. This past April, they raised more than $40,000 through Kickstarter to launch Cherry Bombe. "There's this desire to reconnect, and it's easy to reconnect over food," says Diamond. "You might not run out to buy a new handbag every week, but you do need food every day."
FOOD FORAY: "A great fashion brand is all about the details, and the same is true for a restaurant," says Diamond, who opened Seersucker, the first of three Brooklyn eateries, with her chef boyfriend three years ago. Somehow she also finds time to put out the much-buzzed-about Cherry Bombe with Wu. "Baked"—in all senses of the word—is the tongue-in-cheek theme for their second issue, which covers everything from gluten-free baking to a vegan stoner blog. "We did talk to a few fashion folk about sharing their pot brownie recipes, but no one wanted to go public with them!" says Diamond.
PERFORMANCE REVIEW: "Launching two magazines revealed a sadistic streak in me," Wu jokes. Her partner can relate. "I definitely miss all the comforts that come from working for a corporation," says Diamond. "But sweat equity makes you a stronger person!"


text: Ji Temple Japan hd wallpapers Related Posts:japanese templeswallpaper hd japanjapan templejapanese templejapanesehd japan wallpaperjapan templesjapanese hd picspictures of japanese templestemples in japanShare this:FacebookGooglePinterestTwitterStumbleUpon This wallpaper Ji Temple Japan hd wallpapers has recently added on 9to5 hd wallpapers.com. You can also get high definition hd wallpapers for your desktop backgrounds, iphone backgrounds, tablet backgrounds and other hd devices For Free. To set this Ji Temple Japan hd wallpapers as wallpaper background for your desktop, Click on image to enlarge and then Right click on wallpaper and select the option "Save image as" or "Set As Background" Or Click on Download link Below to save wallpaper on computer. Category : Places Total Download: 9322 Tweet download link : 150 x 150 300 x 225 530 x 397 1600 x 1200 Related For Ji Temple Japan hd wallpapers Twisted Stream Nature Wallpapers Beautiful Building hd wallpapers Sultan Ahmed Mosque Islam hd Wallpapers The beautiful Germany desktop backgrounds Istanbul Turkey City HD Wallpapers Yokohama Bay bridge Japan HD Wallpapers


text: IRONMAN WISCONSIN 2010 View full-size image < previous next > Is this photo a winner? "My husband was participating in Ironman WI in Madison, WI this past Sunday. We had people coming from all over the USA to cheer him on and they all LOVED the T-shirts! We had outlines of all the different states they came from on the back with the title "ROAD TO IRONMAN!" We stood out and got compliments from other athletes, volunteers and spectators! Thanks Custom Ink! We'll be ordering for the next race!" - Team Ebertowski (Sep 14, 2010) Share this photo on: Twitter Facebook Pinterest Similar Photos: sporting event | family | ironman | wisconsin | madison | fun | event sporting Feeling inspired? Visit the CustomInk Design Lab to start your own group order. Design Your Own


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Silver, gold, and copper . . . oh my! Discover all the possibilities that the trendy look of mixed metals has to offer in this 28-minute episode of Beads, Baubles, and Jewels. Plus, learn easy ways to make and use metallic beads to boost your jewelry designs!
Dive into exciting new tips on mixed metal that will have you designs shining in no time! Use metal sliders with inset crystals for a quick, fabulous stretch bracelet with Kristal Wick. Use cubic-right angle weave stitching and super strong thread to create beautiful mixed metal designs with Leslie Rogalski!
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So, here I am at work and BORED!!!! Time for a new blog :)
I have 16 pounds left til goal!!! woo hoo!! well, that was BEFORE this last weekend and BEFORE I had gained a little with the Sunday eat fest haha. But I hope to be back at 156.4 by Sunday morning. I WILL not have a "cheat" Sunday this week!! If I dont stop those cheat days I will never get to goal. But then there's the holidays coming up. Ugh...
Ive slacked on working out this week. I skipped workout Tues and Thur nights
:( But, I WILL workout tonight after my 12 hour shift of BOREDOM and Ill do a last chance workout tomorrow. Gotta get back to 156.4!!
So, as you may have seen I chopped my hair :) The pics I have posted actually are not the latest pics. Ive never cut my hair this short before. When I went and got it cut on Wed I asked for an angled bob. Well, the girl that cuts my hair didnt exactly do what I asked. And she kinda made me a little crooked haha So, a friend from work fixed it for me last night. Cut it a little shorter and angled it a little more. Ill probably go shorter in the back next time because it's not quite angled enough for me. Ill post newer pics later.
Im loving my new hair tho. I figured the new me needed a new look :) And it shows off my clavicles :) hehe
Thinking about maybe giving running another try here and there. I feel like a need to shake it up a bit to get some more weight off. I updated my goals again on SP and it's brought me back down to 1260 cals minimum :( I kinda liked having the 1320 cals haha oh well, gotta do whatcha gotta do! But Im such a stickler for only eating the bare minimum. I get my mind set on the number and freak out if Im over by like 20 cals...I know I have a range for a reason but my mind doesnt work that way. Ill even eat up to 100 cals less so that I dont go over with one food...but then I have my cheat days so I guess Im balancing out...Balancing, not losing. There's the problem.
Anyway, Im loving my size 11 jeans :) I went to Old Navy over the weekend and fit into their 10s. Of course, we all know they run a little bigger but it's still nice to think Im so close to single digit sizes!!! Junior sizes!! :) Thats my goal when it comes to clothes. I hope to fit into at least a size 9 jean. Its funny, cuz I know I want to lose 16 more pounds, but Im not sure how my body is gonna conform with the loss. I carry my weight in my belly pooch and my hips and thighs, so I dont know what size I will be at 140. Itll be exciting to find out.
Is that enough rambling?? I wasted about an hour coming up with stuff to write about haha 9 hours to go! Have a great weekend SP friends!!
So, I decided not to weigh in this morning.
We went to the movies and BD's Mongolian Grill Saturday night. I had one plateful and felt sooo full and sick!! And I fought the urge to eat any Coldstone. Well...last night I fought the urge. Tonight is a whole other story!
I had cinnamon rolls for breakfast this morning and then we ate Long John's for dinner...Then as if I wasnt feeling bloated and guilty enough I had Coldstone. It was a kid's size but one of those has like 750 calories! GEEZ!
Anyway, I didnt do a last chance workout Sat because we went out right after I got off of work and I didnt workout today either. SO, needless to say Im back to it first thing tomorrow!!!
But, you know, even after deciding to eat that dinner Saturday night knowing that Id ruin my weigh in this morning, I stood in the bathroom washing my hands at the movie theater and I looked in the mirror at myself. And what I saw was a thinner, happier me :) I dont usually see a difference in my mirror at home and Im not sure why, but I saw a big difference in that bathroom mirror at the theater and Im very happy about it. I often hear people say I look sooo different but I dont see it or feel it myself (unless in pictures). So, that made my night :) Even tho I proceeded to eat too much today too haha
Anyway, I wanted to confess my eating sins to my SP friends so that I can move on and keep heading towards my goal :) Hope everyone had a healthier weekend than me!! haha
As I had said in a previous blog I have set a "mini goal" for myself to lose 10 pounds by Christmas. I actually would like to reach 150 by then. I needed the motivation of accomplishing something in the near future to get my butt back into gear and it's SOOOO working hehe I am down 4.4 pounds since I set that goal. I have 7 weeks left and 7.2 pounds to go! woohoo!
I was looking at my BMI range and realized the very tip top of my healthy range will be 149 :) Im soooo close!!! So, right now I am overweight but in 8 pounds I will be HEALTHY!!! So exciting :)
I wore a shirt to work today that I bought about 8 months ago. When I bought it (at Aeropostale) I was out with a group of ladies from my mom's church and was in a hurry so I didn't try it on. Looked at it and thought, "It'll fit." Well, I was wrong. haha And Ive been hanging onto it thinking I will definately get into it someday and that day was TODAY!! :) I put it on this morning to see how close I was to fitting into it, like Ive been doing for months, and to my surprise it fit! woo hoo!
Another small victory Ive been noticing is how much easier it is to cross my legs. haha Ive always seen skinny girls cross their legs so easily and theyd be completely crossed! My big old thighs would be in my way and I never felt comfortable. So, just goes to show the smallest things can make a big difference. Makes me happy haha
Still keeping up with my TJ :) Addicted actually...Ive moved onto TJ Cardio Party 3. It's a 50 minute one and Ive added weighted "gloves" to the workout. Im burning 550 calories a workout now :) So, Ive re-adjusted my SP info and upped my calorie intake by just 40 or so calories. Prolly should eat a little more but Im doing ok so far. Ive been adding some strength here and there. TJ Ab Jam or TJ lower body. Those are my problem areas.
Im thinking of posting some "motivation" around the apartment. For those days that Im really wanting the chocolate in the forbidden cabinet :) Im really wanting to lose these last 7.2 pounds by Christmas and get some of this pooch off my belly. That's why my background is what it is :) Hopefully it will serve as some motivation to others :)
Have a wonderful weekend Spark Friends!!
I am SOOOOO happy I could burst!! :) haha I have finally done it!!! Ive left the 160s!! :) never to go back!!! Ive never seen the 150s on the scale before!!!! Great way to start a weekend!!!
feeling strong!!
Im setting a goal for myself. I am determined to lose 10 pounds by Christmas...That gives me 9 weeks to complete my goal. Which means I have to lose one pound a week to be one pound off haha SO, I really have to step it up and get back on track! Ive done really great since Monday and I feel like Im back finally. I got new Turbo Jam dvds thanks to a friend ;) and I think it's just what I needed to get my jump start.
Im using Christmas as a motivator, because I will be seeing family members that I only see a couple times a year on average. And I cant wait to get those "skinny" compliments :) I will also be getting alot of $$$ for gifts and I will be using that money to buy new clothes, because I dont have any winter clothes that fit anymore :) So, I wanna be closer to goal for that reason also.
If I can lose the 10 pounds by Christmas I will only be 10-11 pounds away from my goal. And Ive set my goal date for my birthday on March 2.
I was looking back at the beginning of my journey with SP and was thinking about the things that Ive been doing differently now. Ive been much more slack on "snacking". Ive had the tendency lately to grab a handful of chips from the bag and not measuring it. Then just guess-timating that I ate a serving size or a fourth or whatever. Ive been buying Over the Moon fat free chocolate milk and Ive found myself going to the fridge and just taking a swig of it! no cup, no measuring and then forgetting about it and not recording it! No wonder Im having troubles! Ive also been giving myself more slack on my Sunday "cheat days". Before it was actually a "cheat meal" day rather than a whole day free-for-all. Now it's definately gone off into the deep end of "eat it! it's your cheat day!" NO MORE!! Im gonna get back to counting everything!! and Im gonna get back to one treat a week. AND I have to get back to drinking my water. Ive really slacked on that too.
Im feeling great and re-energized! Im motivated again and cant wait to reach goal! Ive figured out what Im doing "wrong" and Im gonna do this!!
Thanks to all my Spark friends for keeping me going even through a rough patch!! You guys are wonderful motivators!! xoxoxoxoxo


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Retirement Reception for Dave Gillespie
A retirement reception will be held on Tuesday, June 24, 2014, in honor of Dave Gillespie's twenty-nine years of service at the University of Central Missouri. Dave is currently a member of the Office of Technology and was a former faculty member of Electronics Technology. Please join us in honoring Dave at a reception on Tuesday, June 24, 2014 from 2:00-4:00 PM in Union 237B.
For more information:
Contact Stacey Beasley at email@example.com


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Shadows Lyrics - Yo La Tengo
Scold me, that's all you've got to say
Coldly, hurt me and turn away
You say, "I'm not sorry yet" I'm resigned to what is next
I head for the shadow
Hold me taking it back through tears
You've told me, slowly confessed your fears
But I've got myself to protect. It's too soon for me to forget
I'll wait in the shadows
In the shadows
Though I am alone
They help me, see that I'm the only one in your heart
So until I truly believe that your words convey what you mean
I'll wait in the shadows (2x)
I don't mind the shadows.
Click here to submit the Corrections of Shadows Lyrics
Please Click Here to Print Shadows Lyrics
The following area is only for review, if you want to submit the lyrics or the corrections of the lyrics, please click the link at the end of Shadows Lyrics.


text:
Eskom to offer voluntary separation packagesSapa
Power provider Eskom has asked its staff to consider voluntary retrenchment packages, and employees are to decide on participation before February.
Bezuidenhout: They knew I had no degreeSapa
SAA acting chief executive says his colleagues at Mango Airlines and SAA knew he did not have a degree and they supported him.
Zuma not sidestepping duty to answer questions - presidencySapa
Presidency spokesperson Mac Maharaj says President Jacob Zuma is only required to answer questions by invitation, once per quarter.
State says Dewani killers were unprofessional, amateurSapa
State prosecutor Adrian Mopp says were it not for the killing of Anni Dewani, the organisation of the entire plot by the hitmen would be "comical".
ANCYL national congress postponedSapa
This week's national congress of the ANCYL will be converted into a consultative national congress, task team co-ordinator Magasela Mzobe said.
Mango defends SAA acting CEO's academic detailsSapa
Mango Airlines says the academic credentials of South African Airways acting chief executive Nico Bezuidenhout is accurate, contrary to media reports.
Energy minister 'misled' Parliament over PetroSA appointment - DASapa
The DA says Energy Minister Tina Joemat-Pettersson was involved in appointing Tshepo Kgadima to the board of PetroSA; an allegation she denied.
Pirates makes a super comebackSapa
The Orlando Pirates beat SuperSport United 2-1, climbing to fourth place in the Premiership standings.
SAA acting CEO 'overstated his qualifications'Sapa
The airline admitted that it was wrong to state that acting chief executive, Nico Bezuidenhout, had qualifications he didn't complete.
DA considers coalitions for 2016 local government electionsSapa
As it addressed tensions within the tripartite alliance and Parliament, the opposition party says it would contemplate entering into coalitions.


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Peanut Butter Oat Bars
With their yummy chocolate and butterscotch topping, these chewy bars are sure to satisfy any sweet tooth! Since the recipe makes a big batch, the treats are perfect for potlucks and bakes sales.
48 Servings
Prep: 15 min. Bake: 15 min. + chilling
2/3 cup butter, melted
1/4 cup peanut butter
1 cup packed brown sugar
1/4 cup light corn syrup
1/4 teaspoon
McCormick® Pure Vanilla Extract
4 cups quick-cooking oats
1 cup milk chocolate chips
1/2 cup butterscotch chips
1/3 cup peanut butter
In a bowl, combine the butter, peanut butter, brown sugar, corn syrup
and vanilla; gradually add the oats. Press into a greased 13-in. x
9-in. baking pan. Bake at 400° for 12-14 minutes or until edges
are golden brown. Cool on a wire rack for 5 minutes. Meanwhile,
for topping, melt chips and peanut butter in a microwave or
saucepan. Stir until blended; spread over warm bars. Cool
completely; refrigerate for 2-3 hours before cutting. Yield: 4
Nutritional Facts:
1 serving (1 each) equals 120 calories, 6 g fat (3 g saturated fat), 8 mg cholesterol, 49 mg sodium, 14 g carbohydrate, 1 g fiber, 2 g protein.
© Taste of Home 2014


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Book Description:
Handle your California workers' compensation claim and maximize your benefits! From industrial injuries to carpal tunnel syndrome, more than a million Californians a year suffer job-related injuries or illness. For many, receiving compensation can be a nightmare, since recent laws give employers and insurance companies far greater rights and employees fewer medical benefits. California Workers' Comp shows you how to handle a California workers' compensation claim from start to finish. With this plain-English guide, you'll learn how to work with your insurance company to receive the medical treatment and benefits you deserve. Find out how to: file a claim protect your legal rights receive the medical care you need get the benefits you're entitled to deal with uncooperative employers, doctors and insurance agencies negotiate a settlement present your case before a judge Turn to California Workers' Comp to get the benefits you're entitled to. This complete guide can help you whether you're handling your own claim or filing on a minor or other's behalf. This edition is completely updated to cover significant changes in the area of permanent disability ratings, along with recent updates to the Labor Code and other workers' comp laws. Plus, read a new chapter with information tailored to computer users with repetitive strain injuries.
Tags: Reference,


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Ivins tickets are available now for all Ivins events. Whether on vacation or a current resident, you'll never get tired of all the things that are available in Ivins, Utah, and the surrounding areas! Get tickets to Ivins, UT below.
|Event & Venue/City
|May 1, 2015
Fri 8:00PM
|Brian Regan
From $140
|May 2, 2015
Sat 8:00PM
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Film Society
The Johns Hopkins Film Society is a group of students dedicated to providing a fun, enriching experience for anyone interested in film. With weekly meetings and DVD screenings, as well as monthly larger film screenings, students are provided with numerous opportunities to get involved, from advertising, to projecting 35mm film.
Through weekly meetings students are invited to discuss and debate all aspects of film. Meetings provide a forum for members to apply and expand their knowledge of cinema through dialogue with other students. At meetings the topics can range from discussing a specific film, to deconstructing and debating a genre, to discussing filmmakers, to talking about today's box office.
Each semester, Film Society puts on a student-run film series programmed by its members. Film series programmed in past years include "Dante's In-film-o," a series designed to trace out the circles of Dante's Hell with screenings from Eastern Promises to Willy Wonka and the Chocolate Factory; "The Freudian Slips", a series of films each selected to embody a different facet of Freud's psychoanalytic theory, with films ranging from Psycho to Mulholland Drive. Past film series have also highlighted various directors, producers, and actors, such as "Roger Corman's Picks" series. These film series work to expose students to an amazing and varied selection of films, as well as teach them the methods of putting their own successful series together.
In addition to DVD screenings, the Johns Hopkins Film Society puts on monthly 35mm film screenings in Shriver Hall on the 2nd largest screen in Maryland. This provides the rare opportunity for students to learn how to project 35mm film, and to see movies as they were intended to be viewed: on the big screen, with full cinema sound. Recently, Film Society has had opportunities to project a range of films including The Social Network, Coraline, Full Metal Jacket, Misery, and Princess Mononoke.
Programming, advertising, and projecting come together in the spring for The Johns Hopkins Film Festival, an annual festival run and operated solely by students. The Johns Hopkins Film Festival is nationally known, and draws both feature-length and short films from all across the country and world. The festival usually receives close to 200 submissions, from which students select the films to show, and create a schedule of three full days of cinema. Programming and hosting the festival offers students the opportunity to experience an international film festival from in front of the screen, to the festival's innermost workings.
The Johns Hopkins Film Society provides exciting opportunities for students to expand upon their knowledge and love of film. All are welcome. To learn more about the Johns Hopkins Film Festival, check out the Festival website at jhufilmfest.com.
Film Society Executive Board:
Co-Directors: Ian McMurray and Julia Gunnison
Film Programming Chairs: Evelyn Feeney and Annie Rhee
Marketing Chairs: Edmund Nowicki and Kristen Politis
Financial Director: Ned Samson
Head Projectionist: Andrea Massaro
Acquisitions Chair: Danielle Naassana
Film Festival Manager: Sam Brayman
Secretary: Kate Dwyer
Website Manager: Bardia Barapour
Frame of Reference Editors: Hannah Ingersoll and Liz Mattson
Social Chair: Brandon Block
For more information, contact: firstname.lastname@example.com
Frame of Reference
Frame of Reference is the online cinema magazine sponsored by the JHU Film Society. It fosters creative dialogue on the act of filmmaking by offering a place for the publication of cinema manifestos, photography, scholarly essays, fun facts, and film criticism. It is designed to speak to the JHU community.


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Miley Cyrus Showdown: Who Wore Her Best?by Hilton Hater at . Updated at . Comments
It wasn't the most creative Halloween costume, but it was definitely the most prevalent. Especially among celebrities.
Paris Hilton, Kelly Ripa and Crystal Harris all did their best Miley Cyrus impressions this week, with the former rocking out in a bathing suit-like onesie and the latter pair Twerking up a storm.
As far as we know, no wrecking balls were harmed in the making of these outfits.
But who pulled off Miley the best? Compare, contrast and vote now:
Paris HiltonClick Here ToVote for Paris
Kelly RipaClick Here ToVote for Kelly
Crystal HarrisClick Here ToVote for Crystal
Three women dressed as Miley Cyrus for Halloween. Who pulled it off the best? View Poll »
Who does THG vote for? Option D!
That would be Jenny McCarty, who turned this obvious idea into something truly unique.
She went to a party dressed as Miley's TONGUE. It may not be akin to Heidi Klum as a senior citizen, but this is pretty fantastic:
I'm no prudish american, but none.
This is a winner! Very funny!


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Darkman was developed by Twilight and published by Ocean Software in 1991. It was released for the ZX Spectrum, NES, Amstrad CPC and Commodore 64. It was also ported to the Game Boy and Atari ST. The game's plot is loosely based on the film of the same name. In this side-scrolling platformer, the player controls Darkman, a superhero who can jump, kick and punch, as well as swing from a rope during action sequences between levels. In each level, Darkman disguises himself as the boss of the level.
Each day is a new pursuit as the environment develops around you.In this colorful world, Grandma, the dog and a couple of nasty crows are all out to get you! Take to the streets as a fat cat with stolen sausages. Test your reflexes as you zip across rooftops, tippy-toe over power lines and dash along ledges. Swipe to turn, slide and jump to avoid danger while earning coins, special bonuses and power-ups. Challenge and beat your Facebook friends' high score and become the top cat!
Join the millions of players that have made We Rule the longest-running social game on iOS, celebrating over one year of non-stop weekly content updates! YOUR KINGDOM Create and customize your own vibrant world with over 750 buildings and decorations, with new content released each week! Build everything from epic fantasy structures such as Valhalla and the Minotaur's Labyrinth, to real-world landmarks like St. Basil's Cathedral and Buckingham Palace. Expand your Kingdom with additional realm.
Space: the only frontier. With the Earth's resources all but spent, humans have colonized planets in the outer rims of the galaxy. Divided as ever, they battle endlessly among themselves, each world they inhabit a barren war zone. With alliances broken as quickly as they are formed, it's every man for himself. Do you have the will to conquer the galaxy by any means necessary.
MEGATROID is a fun and engaging platform game that lets you type in anything You want and play it! MEGATROID generates its stages and makes it possible for you to play a different one every time You pick up the game! If you're a fan of Megaman or just love really awesome platformers; this is THE game for You![STORY].You play as Hali and your goal is to free the galaxy from the grasp of the almighty machine empire; The Imperium. You are a member of GRiN, the Galactic Rebel Information Network.
Leon the Chameleon is back in "Super Yum Yum: Puzzle Adventures", a Special Edition of the critically acclaimed "Super Yum Yum 3. Download "First Lick" for your first taste of this fantastic puzzle game. About "Super Yum Yum: Puzzle Adventures" "With so many levels it will keep you entertained for some time. A great game for any puzzle fan! 4.5 stars out of 5" Touchreviews.net A fruit monster has run off with your juicy fruit and kidnapped your babies.
Get ready for the ride of your life! Grab your skateboard and jump over countless cute bunnies that are obstructing the roadways of your fair city as you attempt to make it home from school. Face wave after wave of obstacles of increasing difficulty and fight through weather changes as you perform tricks on your trusty skateboard. Collect carrots for bonus points and an extra challenge! Now with retina display support! Grab your board and start jumping over some bunnies today!
COMPLETE THE STACK N TAKE A DEEP BREATH..rnTap to build the shapes and make a complete stack. Balance them and hold your breath with the TIC TIC CLOCK...! Go through the 10 levels and accept new challenge in each level. The game has various levels with funny graphics and sounds. More levels coming in update. The game can run on iPhone/iPod and iPad... Game includes: Gorgeous graphics (Colorful shapes that make stacker stand unique), 10 different levels of varied difficulty.
"OMG I am so addicted to this game. Best of it's kind. I used to play Imob/mafia/knights etc. But this one tops em all! Top of it's Kind - Fantastic game it's the best of it's kind. Great game - Really good game soon as I plyaed it I got hooked and now I am addicted I could believe that this game could be a best seller. Take out rival Families, recruit powerful Associates, and build the biggest Organization in 1940s New York. Features Expand your Organization by recruiting over TWO HUNDRED.


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text:
Pratt's finally did it! They now have a beautiful second location to service all pets and owners in the Surprise / El Mirage area.Read more...
|About Pratt's Pet Store
Pratt's Pet Store has been servicing the valley since the 1940's. We service all kinds of pet owners and breeders. We stock all kinds of pet foods and supplies and we also have many live animals like puppies, exotic birds, chickens, bunnies and reptiles.
Pratt's Pets and Feed History
Emerson Wilcken Pratt 1901- 2001 originally started the Pratt's Feed business. Emerson, originally born in


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November 20, 2014
A well-known local poker player has struck a plea deal in a case involving an online bookmaking operation and will forfeit more than $2.2 million of purported poker winnings.
Richard Henry Lee, who placed sixth in 2006 at the World Series of Poker in Las Vegas, on Tuesday entered a no contest plea to the misdemeanor charge of keeping a place for gambling.
Four of Lee's employees entered the same plea.
Lee and three employees will give up 80 percent of the money, or $2.5 million total, that San Antonio police seized in raids last year.
Police called Lee's home the "nerve center" of the sports booking and betting operation and named him as the bookie.
They raided his house as part of an investigation into a sports betting
Web site that pretending to be offshore.
"I'm glad it's over," Lee said. "Now I need to go win another poker tournament."
One of Lee's lawyers, Alan Brown, said the money Lee is giving up was from the nearly $2.7 million he won at the Las Vegas tournament.
Click Here to access the online Public Inspection File
Viewers with disabilities can get assistance accessing this station's FCC Public Inspection File by contacting the station with the information listed below. Questions or concerns relating to the accessibility of the FCC's online public file system should be directed to the FCC at 888-225-5322, 888-835-5322 (TTY), or firstname.lastname@example.com.


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Book Description:
Combinatorial games are games of pure strategy involving two players, with perfect information and no element of chance. Starting from the very basics of gameplay and strategy, the authors cover a wide range of topics, from game algebra to special classes of games. Classic techniques are introduced and applied in novel ways to analyze both old and new games, several appearing for the first time in this book.
Tags: Science-Math, Mathematics, Pure-Mathematics, Combinatorics,


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Job Title:
Nurse Practitioner (NP, FNP) - Salem
I thought you might be interested in this job
I found this job posted on AANP CareerLink - the best resource today for healthcare careers - and thought you might be interested!
ZoomCare is seeking exceptional Primary Care and/or Urgent Care providers to deliver great care at a modern, state-of-the-art, neighborhood clinics. As a talented Family Nurse Practitioner, you'll diagnose and treat a variety of illnesses and injuries and conduct thorough wellness exams for adults and kids alike.
You'll have quick and easy access to expert clinical support and an array of innovative technical tools for precise and complete decision making. And, like all of our providers, you'll demonstrate clinical excellence, timeliness and superb patient communication as you work alongside an amazing team of professionals that's creating the world's first neighborhood and smartphone-based healthcare system.
Family Nurse Practitioners are Stars at ZoomCare.
ZoomCare believes FNPs are critical to delivering quality care. We've advanced the cause of FNPs by sponsoring legislation that enables our providers to practice with greater autonomy and to dispense prescription medications. And, we support continuing monthly education through Zoom University where providers learn the latest and greatest treatments for a broad range of illnesses and injuries.
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We're looking for the best and brightest FNPs to deliver a healthcare experience that WOWS patients. If you're interested in learning more, please apply here or send a copy of your CV to email@example.com.
Board certified and preferred experience in the following settings; Family Practice, Emergency Medicine, Urgent Care, Pediatrics, Adult Medicine, Chronic Care Management, or Women's Health.
Job Location
Salem, OR, United States
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HEALTHeCAREERS Network is a Dice Holdings, Inc. company.


text:
- Age / Gender:
- n/a, Male
- Location:
- Location not disclosed
- Joined:
- 10/13/08
- All Stats >
User Statshot
- Community Stats
Level 5 Blank Slate
Normal Whistle
Ranked as Civilian
Contact Info / Websites
Recent Game Medals
Square 25 Points Gain at least 500 points Medal Stats.
Spectrum 50 Points Clear the first eight levels. Medal Stats.
Vortex 25 Points Complete 5th level Medal Stats.
This better be worth it! 5 Points Pay off 10% of your mortgage. Medal Stats.
Gone fishing 5 Points Catch 5 fish. Medal Stats.
Handy Man 5 Points Buy your fist tool from the mart. (I'd recommend a fishing rod :b) Medal Stats.
Brave New World 5 Points Start playing. Medal Stats.
Destroyer 50 Points destroy 500 bricks Medal Stats.
Defender 50 Points defend 10 bases Medal Stats.
Demolisher 25 Points destroy 1000 barrels Medal Stats.
Total Medals Earned: 311 (From 56 different games.)
Favorite Games
Star Dust I Rated 3.6726 Stars Stuggle to survive in deep space after the destruction of Earth. Strategy - Other Furiosity Rated 3.6017 Stars Strip away every layer of the game, not mindlessly, but by logical thinking. Puzzles - Other Takeover Rated 4.4119 Stars Lead one of 3 nations in the battle for Rivadis in this fantasy real-time strategy game. Strategy - Real-time (RTS) Mastermind: WC Rated 4.4368 Stars Manage your minions, defend your base, and conquer this puny planet ONCE AND FOR ALL! Strategy - Real-time (RTS)


text: We're in favor. Gruffydd and Marion -----Original Message----- From: Loch Ruadh Seneschal <seneschal at lochruadh.org> Sent: Friday, May 21, 2010 9:07 AM To: Shire of Loch Ruadh <loch-ruadh at lists.ansteorra.org> Subject: [Loch-Ruadh] Hosting Roundtable PLEASE READ AND RESPOND - VERY IMPORTANT! We have an opportunity to host the upcoming King's Roundtable in July. Alric has found a site - the Student Union at TCU. This is a very minimal effort event to put on, but very important to the business operation of the Kingdom. In general, people have voiced support for us doing this in the past, but as the bids for the event are past due, and need to be to the Kingdom Senschal as soon as possible, we can't wait until Populace Meeting next week to vote, so I am asking for an email response on the question: Should Loch Ruadh submit a bid to host Roundtable in July? Please respond today - the earlier the better. Let other members of the Shire who might not have see this missive yet know about it and encourage them to respond today as well. I have prepared a bid, and if the general consensus is yes, I will submit it. In Service, Padraig Seneschal _______________________________________________ Loch-Ruadh mailing list Loch-Ruadh at lists.ansteorra.org http://lists.ansteorra.org/listinfo.cgi/loch-ruadh-ansteorra.org


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|Product Image
|Item Name+
Web Spinner DVD - Amazing JoeJoe
This is the DVD thread workers around the world have been waiting for. The Web Spinner DVD is finally ready and it's a winner! Starring none other than the creator of the Web Spinner,...
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Woofle Dust - by Scarface
Snort a gigantic pile of unknown white substance straight up your nose, with the greatest of ease! Everyone knows magic doesn't work by itself, sometimes it needs a little help from a mysterious...
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WWW - World Wide Wonder
Picture this. You write something on the back of a napkin which remains in full view throughout. A card is selected and returned to the deck. You explain that like everything else,...
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X-Ray Vision : Subtle Mysteries #1
Imagine being able to see through playing cards, as if you had X-RAY EYES like Superman! This diabolical secret allows you to look at the back of a playing card and reveal the color of it's...
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X2 Stealth Hookup
Suspend borrowed objects on the tip of your finger! Pens, silverware and much more can be balanced in an impossible way, under bright lights just inches from their eyes. The object defies...
>> More Info


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Twenty Safelite AutoGlass® employees will travel to England this week to compete alongside 900 Belron® colleagues from other countries in the London Triathlon.
Columbus, Ohio - Twenty Safelite AutoGlass® employees will travel to England this week to compete alongside 900 Belron® colleagues from other countries in the London Triathlon. The event, hosted by Belron®, Safelite's parent company, raises money for Ma'Afrika Tikkun, an organization benefiting children and orphans in South Africa.
The Safelite AutoGlass® competitors are:
The twenty participants were selected for the all-expense paid trip based on their performance in the inaugural Safelite® Triathlon and Poker run in Las Vegas. The April event featured more than 150 participants who raised $165,000 for Ma'Afika Tikkun.
In 2009, more than 600 Belron® employees from around the world took part in the London Triathlon and raised $110,000 for Ma'Afrika Tikkun. Belron® has been involved in the triathlon for eight years and this is the forth year that Safelite AutoGlass® has participated.
The Safelite® athletes will present a check to Ma'Afrika Tikkun upon completion of the event for $225,000 which includes sponsorship money collected by the athletes themselves. The remainder of the donation is made up of contributions by company leaders and a corporate gift.
"We're thrilled to once again be represented in the London Triathlon. Not only are the individuals of Team Safelite® outstanding athletes, they are excellent philanthropists. They've trained and worked very hard to prepare themselves to perform well in this event—all for the benefit of others," said Tom Feeney, president and chief executive officer of Safelite® Group. "The London Triathlon is a wonderful opportunity to meet other members of the Belron® family from around the globe and demonstrate Safelite's commitment to be responsible members of the global community."
Thousands of athletes are expected to compete in the Triathlon August 7-8 in London. For more information about the London Triathlon, visit londontriathlon.co.uk. For more about for Ma'Afrika Tikkun, visit maafrikatikkun.org.za.
About Safelite AutoGlass®
Safelite AutoGlass®, founded in 1947, is the nation's leading provider of vehicle glass repair and replacement services, providing mobile service to more than 95 percent of the U.S. population in all 50 states. The Columbus, Ohio-based company employs 10,000 people across the United States and serves more than 3.8 million customers each year through its company-owned operations.
For additional information, please contact our Media Relations department.


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Asserting that New Jersey's tax collection process unfairly burdens municipalities, Evesham's township council said it would distribute no tax revenues to its school and fire districts until they agree to discuss sharing collection costs.
"I believe we need immediate discussions with these entities," Mayor Randy Brown told the council Tuesday night.
The panel voted unanimously to withhold the next round of payments to the Lenape Regional School District, the local school district, and Evesham Fire-Rescue.
Township officials project that Evesham will have to allot about $3.4 million this year for the collection of taxes, unpaid taxes, and lost property tax appeals.


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Tag Archives: NFL numbers
NFL Betting By the Numbers: Week 1 Numbers Of Note
When you bet on the NFL, you should know all about the numbers. Numbers for stats, numbers for trends, numbers for betting lines, numbers for 'totals' – You should know it all. Here at Bang the Book, we are set to talk about the football betting lines for Week 1. Here are the most important numbers that you need to remember for the week! 0: The number o.....


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The nominees for the 2012 BET Awards have been announced on Tuesday, May 22. In movie category, critically acclaimed "The Help" and George Lucas-produced "Red Tails" are facing off each other for the coveted Best Movie gong.
Meanwhile, "The Help" star Viola Davis and "Colombiana" actress Zoe Saldana are competing for the Best Actress title. As for the Best Actor category, "Iron Man 2" actor Don Cheadle will face off "Thor" star Idris Elba.
Along with the nominees announcement, BET confirmed that "The Avengers" actor Samuel L. Jackson has been sealed to host the awards ceremony. Slated as the musical performers are Nicki Minaj and Chris Brown.
Of choosing Jackson as the host, BET Networks' president of music programming and specials Stephen G. Hill said, "We are massively excited about having Sam Jackson, the world biggest box office star, host the BET Awards 12." He added, "His wit, presence and gigantic personality are the perfect match for our show."
BET Awards 2012 will be held on Sunday, July 1 at the Shrine Auditorium in Los Angeles. For this year, the Cadillac Lifetime Achievement honor will be given to Maze featuring Frankie Beverly.
Best Movie:


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The pound stayed higher versus the dollar and the euro after the European Central Bank cut its benchmark interest rate to a record-low 0.5 percent.
Sterling gained 0.2 percent to $1.5582 at 12:49 p.m. London time and strengthened 0.1 percent to 84.66 pence per euro.
ECB policy makers meeting in Bratislava today lowered the main refinancing rate to 0.5 percent from 0.75 percent, a move predicted by 45 of 70 economists in a Bloomberg News survey.
To contact the editor responsible for this story: Mark McCord at firstname.lastname@example.com


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