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Old Navy
Everything you love about Old Navy is available in women's plus (sizes 14 to 30). The "shop by outfit" section puts together stylish, chic looks—at pretty incredible prices.
more on luckymag.com
Your First Look At Rita Ora In Fifty Shades Of Grey
Film stills released earlier this week show the singer in her debut acting role, Mia Grey.
Dear Lucky: How Do I Wear Wide Leg Pants?
Wide-leg pants are—well, they're huge. "Wide-leg is a timeless alternative to trousers or jeans," said style star Nicole Warne, who likes to pair them…
Rashida Jones Has Purple Hair Now
Rashida Jones performed hilarious holiday medleys on Jimmy Fallon—and showed off new purple highlights in her hair.
WATCH: The Black Friday Chaos Begins!
All over the country, people flocked to stores as early as 6 pm on Thanksgiving in hopes of finding incredible Black Friday deals.
15 Last-Minute Black Friday Scores To Buy Without Leaving Your Bed
Shopping Black Friday sales online means you don't have to brave the crowds to get a good deal. Here are some of the best bargains we could find on fa…


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Brackets has closed. The restaurant that earned a scathing review from Hanna Raskin just over a year ago clearly never solved its identity issues. Raskin trashed nearly every dish, likening the crust on a passable pizza to Chuck E. Cheese's, referencing a bloody and cold steak and calling nachos li ... More >>
Two weeks ago we told you how the Arts District Food Truck scene was moving again, this time to the plaza outside the Winspear Opera House. Today I dropped in to see how the trucks are settling into their new location. Five trucks were parked under a cloudy May sky, catering to office workers in the ... More >>
Photos by Anna MerlanThis morning the Dallas Contemporary was closed to the public, but a bunch of reporters were inside taking first looks at the most exorbitantly priced, publicity-getting items, otherwise known as the Fantasy Gifts from Neiman Marcus's 2011 Christmas Book. Eight of t ... More >>
Brackets' pizza isn't bad, but it's not what we expect from Il Cane Rosso.Each week, Happy Eating highlights a different restaurant happy hour food menu, letting you know what deals are out there. This week, we try out the Il Cane Rosso pizza while tuning up our ping-pong game at Brackets. ... More >>
The Asian Film Festival has promise
You want answers to last week's quiz? We got answers.
Answer all correctly and win a Def Leppard album
Like the real thing, Table Tennis shouldn't be played alone

subset: commoncrawl


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Les abricots
Yesterday, we found a basket on our fence. The third this week. It's made of osier and hung by a metal hook. Inside, we could see apricots. And at times, cherries. Most… Read More
"Busy", for ever and more
Trying to finish the book keeps me busy. But I have the feeling, someone – not to name anyone, Lukie – is going to keep me even busier. She/he's the tiniest thing… Read More
All mornings should be like this – Custard-filled cornbread
Yesterday, two am. Tonight, we ate al fresco. In our garden. Who said you're not allowed to play make-believe anymore? I made dessert. One strawberry tart, only it's so much more. Black… Read More
Réussir le fondant pâtissier blanc, pas à pas – Mastering white pâtissier fondant, step by step
One of the first things you see through a boulangerie-pâtisserie window in France is a herd of glazed éclairs and choux. Pretty in pink, brown, white, and more often than not, green… Read More
Comme la rosée des matins d'été – Confiture de concombre à la vanille
[Not unlike summer morning dew – Cucumber and vanilla jam] I walked in mud and bought some vintage tupperwares at a vide-grenier. I saw waves bigger than life. I felt them too.… Read More
Surprise, surprise – Madeleines aux framboises
[Raspberry madeleines] This morning, the sky turned black, of the bruised kind. And then, clouds started to grumble, roar really. For minutes. And before we knew it – {insert French accent here}… Read More
Not unlike living in cotton – Scones, scones, scones…
So it seems I've told you I'd see you soon with tips for the perfect scones. Apparently, soon can hold different meanings. A birthday to the sound of drum n' bass, and… Read More
On clouds blanketing everything we see – Roast chicken and root vegs
An hour ago, I took a whole – 1,5kg kind of whole – chicken out from the oven. Just for myself. You see it started this morning when I first opened my… Read More
Everyday magic
I made scones today. Twice, because two seems to be a trending number these days. Same recipe, except for five extra grams of milk. And somehow, no matter how long I have… Read More
Bonjour avril
[Hello April] My weeks have been made of Sundays lately. And it really feels like walking with my eyes closed and crossing my fingers at every step I take. Not unlike getting… Read More
The end – Coffee and chocolate self-saucing cake
The end. Of this, that is. I was writing when the timer went off. Upstairs, sitting cross-legged in front of my laptop. It wasn't as cold as the outdoors would suggest. Perhaps,… Read More
Through the wrong end of a telescope
I look through the window. And this is all I see. Rain and trees that snow. The very spectacle of April happening before my eyes. But no matter how breathless it makes… Read More
Bonjour mars, au revoir mars
[Hello March, goodbye March] I've felt raindrops running through my hair; and my dress too. I've made a cake. And another one too. I've seen blossoms on every tree. I've walked in… Read More
Find a map and draw a straight line – Chewy flapjacks
I started collecting objects, to make up for memories I forgot. A blue pool ball, a broken cigarette, a plastic table number. I read words. Most of the time, at night. Yes,… Read More
She fell in love with…
PS. Not of the one thousand and six hundred pounds kind (dreams). More of the tilt my own lens in front of the sensor. And yet, it feels just right. … Read More
Those three words – Gelée d'huile d'olives
[Olive oil jelly] In autumn, with figs, a young brillat-savarin curd, and a warm sponge so full of vanilla seeds it's almost grey. Perhaps, a few toasted and salted almonds for crunch.… Read More
On breakfast for dinner – Bacon and egg sandwich
When home means finally-untangled hair, the sound of waves that won't quit our minds – oh and of the shower he's into two-open-doors away while this song is playing on repeat, only… Read More
La vanille
It's hard to play favourite with vanilla. Tahitian vanilla (or for the geeks out there, and that includes me, Vanilla tahitensis) is a bit of a outsider – considered its the only… Read More


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Items In Cart
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- 7-Keto
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- Mushrooms
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- Probiotics
- Propolis
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- Red Yeast Rice
- 21st Century Health
- California Gold Nutrition
- Carlson Labs
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- Doctor's Best
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- Healthy Origins
- iHerb Brands
- Jarrow Formulas
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Customer Reviews
EGCG + Cafeine perfect combination
1 person found the following review helpful
Posted by 5199175693819634756 on Sep 06, 2013
Purchased at iHerb
Nice product form Now Foods, I combine it with caffeine in order to boost myself and burn calories!
Best egcg EVER !!!!
1 person found the following review helpful
Posted by Sickness-ELIMINATOR on Aug 11, 2013
Purchased at iHerb
Love my Green Tea!! I was taking green tea extract for about three months and I did like it, I think it helped me lose some weight I was trying to shed. I then started working with a personal trainer (she's amazing!) who also has a masters degree in nutrition. She suggested I try taking Yohimbine with my green tea extract. I had heard of Yohimbine at this point but had no experience with it. From the research I've done, my understanding is that it blocks Alpha2 activation allowing for greater fat breakdown to occur and also increase blood flow to adipose tissue, making it perfect for targeting stubborn fat. And when taken with green tea (or caffeine but I prefer green tea) the effects are multiplied. . . . . You can find other products I use on my Page! I use only products that have Scientifically Proven benefits.
Ultimate Cancer Prevention
1 person found the following review helpful
Posted by Reviewer2224762 on Apr 23, 2012
Purchased at iHerb
It's a strong standardized supplement and a major step for cancer prevention. Would require drinking 10 cups a day to get this much EGCg. Everyone should seriously consider.
seems like good stuff
1 person found the following review helpful
Posted by Reviewer1007546 on Jan 30, 2012
Purchased at iHerb
It´s hard to evaluate a product that isn´t suppose to have any noticiable effects. I use it as an antioxidant and like it.
Grean Tea extract
1 person found the following review helpful
Posted by Reviewer1761684 on Feb 27, 2012
Purchased at iHerb
I had to search for a while to find grean tea with the 400mg. I am happy with my purchase. I had no real expectations for this supplement I am taking it beacuse of a book I read. I like not having to taste it. I have tired several grean teas in liquid form and it is hit or miss on wether or not they taste good. I dont worry with these.


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Wii Archive
Watch TV, Movies on WiiJapan's Wii no Ma service to include movie and TV show rentals. Article from Gamersyndrome.com Tweet
Black Wii Launches in EuropeWasn't this thing supposed to be available at the time of the Wii's launch? Article from Gamersyndrome.com Tweet
Wii Balance Board ReviewThe Wii Balance Board has become one of the most popular accessories for the Nintendo Wii, due to its use...
Mass: We Pray Video – Creator RevealedSome of you may have been wondering about who was behind the "Mass: We Pray" Wii parody that turned up...
Umloud!: Music, Gaming, and Charity EventHarmonix and Child's Play will be hosting a charity event featuring Rock Band 2. Article from Gamersyndrome.com Tweet
Shiren The Wanderer to be Released for WiiAtlus has announced that a new Shiren the Wanderer game will be released in for the Wii on February 9,...
Monster Hunter Tri Lecture, New ScreensCapcom gives a lecture on the basics of Monster Hunter. New details and screenshots of the game inside. Article from...
Final Fantasy Crystal Chronicles: The Crystal Bearers AnnouncementFinal Fantasy fans get ready for a whole new game play experience. Set to be released on Wii November 5th...
Final Fantasy XIII Screenshots and Wallpaper CollectionHere is a collection of press released Screen shots and Wall papers from Square Enix. Who cant wait for Final...
Wii Turns ThriiIt's been three years since Nintendo launched its shiny white console. Article from Gamersyndrome.com Tweet
Phoenix Wright to Serve Justice on WiiWarePhoenix Wright will soon object on the Wii. Article from Gamersyndrome.com Tweet
New Zelda Wii DetailsZelda producer Eiji Aonuma says Zelda fans can expect some surprises with the upcoming Zelda on the Wii. Article from Gamersyndrome.com Tweet


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Monster Energy Drink
The official product was launched in the year 2002. It has risen from the darkness of an unknown product to the top profiting earning product in the energy drink category. Within three years, the brand came up and climbed to the top of the rankings in the drinking category. It had nested the brand with the top rock stars. During the year 2005, stockholders witnessed about 333% gain from a share that was worth $18.20 with an amazing $78.81 during the end of the year. It is a multimillion dollar company that's has a very firm grip on the energy drink revolution. The marketing tactics that they implement on its customers is the real reason for the success of the energy drink.
Monster Energy Drink Ingredients:
Ingredients:[6] carbonated water, sucrose, glucose, taurine, sodium citrate, color added, panax ginseng root extract, L-carnitine, caffeine, sorbic acid, benzoic acid, niacinamide, sodium chloride, glucuronolactone, inositol, guarana seed, pyridoxine hydrochloride, sucralose, riboflavin, maltodextrin, cyanocobalamin.
Monster Energy Drink Products:
- Original Monster Energy,
- Monster Lo-Carb,
- Monster Assault,
- Monster Khaos,
- The Monster Juice Hybrid M-80,
- Extra Strength Monster,
- And Java Monster,
- Premium Coffee Supercharged With Our Killer Monster Energy Blend.
Tools For Marketing:
Monster energy drink is very fortunate enough to have the largest budgets for promotional ventures in the realm of competition and advertising. The marketing strategy that they apply makes it different from the rest of the competitors. The market for targeting are the males from teen age to the ages of forty who are following a lifestyle with action, sports, motor sports that are very much popular. While targeting these people, it is very much important that appeal should be natural and ambitious.
Facebook Profile
This is how Monster tries to capture the attention of the people. The tools that Monster uses exaggerate and enhance the natural male reactions. They force the false image of opposite sex deep into the minds of men. It is not just attractive women who are used are the main reason for the marketing strategy. They also use the allure of action sports realm. This is another reason for the Monster promotions. This brand is known for the high dollar sponsorship of large events in different aspect of sports.
They have sponsored some events like the X games, Dew action sports tour, the Monster Energy pipeline Pro and much more. These provide important outlets for creating hype for the product. This gives the athlete the edge over winning the competition. Their logo is one of the key advantages for athletes to strive. All these allurement and hype that is created causes the market to fall into the feelings of envy and jealousy for the professionals. They have made a stage that teaches people that though the Monster energy drinks they can attain the skill and energy that the heroes have attained.
Sponsorship Programs:
Through the sponsorship programs, Monster has established over the internet. It has become possible that by drinking the energy drink they can achieve to the fame that they desire. For getting a sponsorship from Monster, all you have to do is submit a simple resume that provides information about your sports and in which sport you excel and some pictures that will entertain the audience. Through these sponsorship programs it has become very popular by creating a market with two categories. They are "those who can" and "those who can't". These are some strategies that add fuel for jealousy that burns within men and enforce the concept f natural male dominance.
Free Promotional Giveaways:
Monster Energy drink Company finds a way to spend every dollar in the budget to help in selling the product. The best way that they do is through the age old technique of giving free promotional giveaways. It can be found in the countless contests that they put regularly that involve in giving the winners the chance of where they feel the glory and fame. This sticks the hopeful envy that the Company draws on in every aspect of the marketing campaign. Another campaign is that in all events Monster host and they are merely present in various places with the energy drink. This is the right game of marketing where they not play with human feelings but with the psychological functions instead.
The attitude, values and morals that a man gains not only affect himself but also affects other people around him. It is very easy for people to get lost into the world of allurement and it is not that easy to escape from it. Monster is continuing to have a rise carrying the weight of target market aspirations with them. The name Monster is just very truly fitting for the product which means 'unleash the beast within".


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How we react during every major Apple announcement
This video hit close to home. It's almost as if they were monitoring the TUAW newsroom during this year's WWDC keynote. Thankfully we're not being bugged, this is just a dead-on recreation of how Apple fans react during every major Apple event. The folks at Slacktory have nailed the highs, lows, and stunned disbelief that there wasn't one more item covered. You're probably not alone in your reactions.
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Software Updatesmore updates
- Vizzywig 8xHD price tag now a very affordable $49.99
- Automatic targets teen drivers with License+ service
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- YouTube for iOS gets updated with full support for iPhone 6 and 6 Plus
- iOS 8.0.1 update now available (Updated -- Don't update!)
- NFL Mobile updated for 2014 Season with new Fantasy Football features, NFL Now integration


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For immediate release – 8/29/12Contact: Rita Elliott, Director of Communications, 815-226-3374
The College provides more than $7 million of institutional aid each year to its students. The affordability of education is one of the most a pressing issues in higher education. Rockford College is proud of the educational opportunities it provides and works tirelessly to preserve and increase the value of that education in the face of rising costs. The continued support and growth of the College's scholarship programs are critical to students 'continued success and is why the proceeds from this year's event will benefit students directly.
Also included in the black-tie event is a live auction hosted by Paul Logli, 50/50 raffle and dinner and dancing to the music of Harlan Jefferson and his band.
Tickets are $150 each. For questions or more information, please call 815.394.3756.
11/21/2014 » 12/12/2014PULLED: Art & Design of the Screen Printed Image
12/1/2014Student Music Recital
12/4/2014EDP: Seminar #6 - Conflict Management and Resolution
12/4/2014NICNE Customer Service Workshop
12/4/2014FORUM: Sports Management Symposium
11/20/2014Senior Development Officer and former mayor John McNamara honored with Hero of Homeless Award
11/20/2014'PULLED: Art & Design of the Screen Printed Image' art exhibit opens Nov. 21
11/13/2014English professor and poet Dr. Susan Porterfield works featured
10/30/2014Fall 2014 faculty activity features diverse work
10/27/2014Rockford University recognized for excellence in television advertising


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Sign up for Yardbarker's daily Top 10 newsletter, the Morning Bark! Customize to get news on your favorite sports and teams.
Reds news: Cincinnati signs OF/1B Shelley Duncan and C Lou Marson
The Cincinnati Reds have continued to stay active in the free agent market during the regular season picking up first baseman/outfielder Shelley Duncan and catcher Lou Marson, signing them to minor league deals. Both players had been released by their former teams either during or after spring training; Duncan from the Diamondbacks and Marson from the Phillies.#Reds sign 1B Shelley...
Via Sports Rumor Alert
May 10, 2014 | Discuss
Shelley Duncan raises the steaks with minor-league HR
Shelley Duncans stop at Triple-A Durham has included a savory prize: Free steak. Duncan, who went 10 for 55 (.182 batting average) with two home runs and six RBI in 20 games with the Tampa Bay Rays this season, smacked a solo home run to left field in the second inning Sunday during the Bulls 7-4 victory over the ScrantonWilkes-Barre RailRiders. Yes, this lacked the drama of...
Via Fox Sports Florida
June 02, 2013 | Discuss
Rays add Shelley Duncan to roster
Luke Scott is out, and Shelley Duncan is in. The Tampa Bay Rays added Duncan to the Opening Day roster Sunday, a day after placing Scott, a designated hitter, on the 15-day disabled list. Scott sustained a right calf strain last week, which he said was caused by drinking too much alkaline water. A big part of the equation for us was how long we thought Luke was going to be out...
Via Fox Sports Florida
March 31, 2013 | Discuss
Hooters Ball Girl Knocks Down Fair Ball And Gives It To A Fan (Video)
Spring training is usually the time for players to knock off the rust and, hopefully, make the majority of their mistakes. This year, even the ball girls got in on the gaffes. The Philadelphia Phillies and the Tampa Bay Rays were in the top of the sixth at Bright House Field, where the Phillies play their spring games, when Tampa's designated hitter, Shelley Duncan, hit a ground...
Duncan's Slam Wiped Out By Six Unanswered For Athletics
The Indians opened up a three-game set in Oakland last night against the relevant again Athletics. The Indians struck first in a big way, but their starting pitching failed them again as did the bullpen while the offense powered down after the fourth inning in a 6-4 loss. Zach McAllister started out strong, and for the first three innings, pitched like the guy who has quickly become...
Via Waiting For Next Year
August 18, 2012 | Discuss
Congratulations to Shelley Duncan and Family on Birth of Twins
Shelley Duncan wasn't in on the hit parade yesterday. Today, he got a double. Shelley and wife Elyse welcomed twin boys (William and Walker) today. According to the team, mom and babies are doing fine.
Via Waiting For Next Year
July 05, 2012 | Discuss
Much Ado About Duncan
Earlier this week, two of my favorite Tribe Scribes—Jordan Bastian of MLB.com and Paul Cousineau of the The Diatribe—weighed in on the left field situation in general, and one David Shelley Duncan in particular. It seemed, to my chagrin, they were disagreeing on Dunkers' value as an everyday player and his viability as the team's regular left fielder for the 2012 season...
Via Waiting For Next Year
March 08, 2012 | Discuss
While We're Waiting…Kotchman vs. Duncan, Urban Recruiting, Cavs Contracts
While We're Waiting serves as the early morning gathering of WFNY-esque information for your viewing pleasure. Have something you think we should see? Send it to our tips email at email@example.com. The Kotchman signing and what it means for Shelley: "However, I can't shake the feeling that the Indians have found real value with Shelley Duncan and it's a shame...
Via Waiting For Next Year
February 05, 2012 | Discuss
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Russian Navy "Admiral Gorshkov" aircraft carrier converted to the Indian Navy's "INS Vikramaditya"
Бывший ТАКР ВМФ России (Баку) Адмирал Горшков для ВМС Индии Викрамадитья
By: PConvictions | Comments: 9 | Views: 8050 | Votes: 0 | Shared: 12
Leaked: Nov-24-2010 in Arts and Entertainment
YAK-41 Crashes On Carrier landing Test
Rare video The Yak-41 is a supersonic V/STOL (Vertical/Short Take-Off and Landing) naval fighter. Although it never entered operational service, some of it's advanced technology will see applicatio
By: Private-Parts | Comments: 0 | Views: 71314 | Votes: 3 | Shared: 10
Leaked: Oct-25-2009 in Other
THE PROVING GROUND: Vikramaditya aircraft carrier
The Proving Ground show visited "Vikramaditya" aircraft carrier during her last sea and flight trials in the Barents Sea prior being handed over to the Indian Navy. INS Vikramaditya is the former Sovi
By: Titantc | Comments: 30 | Views: 884 | Votes: 5 | Shared: 3
Location: India | Leaked: Jun-9-2014 in Weapons, Vehicles
F-22 vs T-50 PAK FA (copied or not?) F-35B vs Yak-43 (copied or not?)
Of course not. Russian's never had F-22 on their hands to copy it from and only an idiot would think that legendary designer's bureau such as 'Sukhoi Irkut Corporation" would copy a multi-billion doll
By: AgitProp | Comments: 135 | Views: 10435 | Votes: 4 | Shared: 48
Leaked: Nov-25-2013 in Weapons, Vehicles, Science and Technology
[Pix and Vids of NEW military hardware] While Obama is shutting down our government, Russian Ground Forces to Receive 750 Units of Military Hardware i
...750 Units of Military Hardware in 2014, as well as 36 NEW Military Ships by End of 2013! MOSCOW, October 1 – The Russian Ground Forces will receive over 750 units of military hardware in 20
By: AgitProp | Comments: 107 | Views: 2802 | Votes: 4 | Shared: 0
Location: Russia | Leaked: Sep-30-2013 in Weapons, Vehicles, Propaganda
[Great Pix of Navy Ships] Russian Navy to Receive 36 Warships in 2013
ST. PETERSBURG, July 7 – The Russian Navy will receive 36 warships in 2013, an unprecedented number in Russia's history, Navy Deputy Commander-in-Chief, Vice Admiral Alexander Fedotenkov said on
By: AgitProp | Comments: 19 | Views: 3433 | Votes: 0 | Shared: 0
Location: Saint Petersburg, Saint Petersburg City, Russia | Leaked: Jul-9-2013 in Regional News, Weapons
Russian Navy to Receive 36 Warships in 2013
ST. PETERSBURG, July 7 (RIA Novosti) – The Russian Navy will receive 36 warships in 2013, an unprecedented number in Russia's history, Navy Deputy Commander-in-Chief, Vice Admiral Alexander Fedote
By: AlexanderSigal | Comments: 30 | Views: 1472 | Votes: 3 | Shared: 0
Location: Russia | Leaked: Jul-7-2013 in Other News, Weapons, Vehicles
Indian Carrier Starts Final Trials in White Sea
A Russian-built aircraft carrier refitted for the Indian navy started final sea trials on Friday in the White Sea, the Sevmash shipbuilder announced.The Vikramaditya (formerly Admiral Gorshkov), which
By: USMC_SRT | Comments: 27 | Views: 1461 | Votes: 0 | Shared: 0
Location: Saint Petersburg, Sankt-Petersburg, Russia | Leaked: Jul-7-2013 in Other News, Creative, Weapons, Citizen Journalism
Russian Navy to Receive 24 Subs, 54 Warships by 2020
MOSCOW, March 11 (RIA Novosti) – The Russian Navy will receive 24 submarines and 54 warships of various classes by 2020, Defense Minister Sergei Shoigu said on Monday. "As a result of the imple
By: AlexanderSigal | Comments: 74 | Views: 1460 | Votes: 2 | Shared: 0
Location: Moscow, Moskovskaya oblast, Russia | Leaked: Mar-11-2013 in Regional News, Weapons
India to ink Rs 25,000 crore In Sukhoi and helicopter deals with Russia
India will soon ink defence deals worth around Rs 25,000 crore with Russia, including two mega ones for 42 more Sukhoi-30MKI fighters and 59 additional Mi-17 V5 armed helicopters. This will reasse
By: yash | Comments: 56 | Views: 1959 | Votes: 0 | Shared: 0
Location: India | Leaked: Dec-22-2012 in World News
Delhi looking to revive India-Iran-Russia axis to influence Afghanistan
Delhi looking to revive India-Iran-Russia axis to influence Afghanistan * Singh set to meet Putin, convince Moscow to engage more in Kabul By Iftikhar Gilani NEW DELHI: India is keen to reviv
By: angloirishcad | Comments: 8 | Views: 8269 | Votes: 0 | Shared: 0
Leaked: Mar-18-2010 in Iran, Afghanistan, News
Crazy Russians tests MIG-29 K/KUB Aboard the Kuznetov Carrier
MOSCOW: Russia's MiG aircraft maker said it has successfully tested four MiG-29 jets due to be delivered to India. "During the tests Sep 28-29, the MiG-29K and MiG-29KUB fighters conducted sever
By: Jattdadanda | Comments: 18 | Views: 10559 | Votes: 1 | Shared: 1
Location: Livingston, New Jersey, United States | Leaked: Oct-7-2009 in News
Search results for Admiral GorshkovSort by:
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DJ Felli Fel - Reason to Hate (Ringleader Remix)
Artist: DJ Felli Fel
Featuring: Ne-Yo, Wiz Khalifa, Tyga
Producer: Mikhail and Pops [for Ringleader Music Group]
Album: None (Promo Single)
Avg Rating: 3.6 ( 14 votes )
Whether valid or irrational, Debbie Downers will always find something to pick at. Instead of trying to silence the haters, though, DJ Felli Fel eggs them on with a cooked up remix to his single Reason To Hate. While the vocals work of guests Ne-Yo, Tyga, and Wiz Khalifa remain unchanged—each riding with their energetic, recognizable vocal styles—the fluffy beat is given new life. Producers Mikhail and Pops [for Ringleader Music Group] add some bells and whistles (trap effects, a few synths, and more) to the track, breathing some more life into the song with a vibrant, cascading feel. There is no word on anything more from Felli Fel, but be sure to compare this with the original, featured originally in April.
DJ Felli FelNe-YoWiz KhalifaTyga HitmakerClub
Written by Lucas G. on 07/28/13
Submit your Rating
Rock Hill Productions
First DJ Booth Appearance:
"Get Buck In Here ft. Akon, Diddy, Ludacris & Lil Jon" (2007)
Total DJ Booth Features:
9 Songs
You Might Like...
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Method and system for reducing feeder circuit loss using demand response
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Baseball recently collided with music when Arizona Diamondbacks pitcher Bronson Arroyo, infielder Aaron Hill and outfielder Mark Trumbo paid a visit to Fender's headquarters in Scottsdale, Ariz.
Each player is an avid musician, so they were excited to see the memorabilia and some of the products being developed around the facility.
"I love coming to places where they're actually inventing things," Arroyo said. "You can just tell that they're having a good time here. It's just fun to come in and see the creativity."
In addition, the guys told a few stories of meeting some of their musical idols, like Rush's Geddy Lee and members of the Red Hot Chili Peppers.
Watch the Diamondback heroes tour Fender's home base below and click here to see more of Fender's limited-edition Major League Baseball Stratocasters.


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Pulitzer Prize-Winning Photojournalist Brian Lanker Dies at 63
Posted by David Ozanich — 21 Mar 2011
Pulitzer Prize-winning photojournalist Brian Lanker has died at the age of 63 at his home in Eugene, Oregon. While his work has appeared in many publications, from Life Magazine to Sports Illustrated, he is perhaps best known for his photograph "Moment of Life" (seen above) which was part of a black-and-white photo essay about childbirth. It was project which won him the 1973 Pulitzer Prize while he was working at the Topeka Capitol-Journal.
Interestingly, he later married the woman, Lynda, who was the subject of "Moment of Life". He is also known for his book "I Dream a World: Portraits of Black Women Who Changed America".
From the Chicago Tribune:
"Brian was a master craftsman who didn't need words to communicate," Register-Guard's editor and publisher, Tony Baker, said. "His camera work alone made for extraordinary storytelling. He was a consummate professional, always prepared when on assignment for the paper. He was a big personality with a big-picture view of life and of his craft. Brian made everyone around him better at what they did."
From the New York Times:
As a freelancer in recent years, Mr. Lanker took on high-profile clients to finance the documentary work he thought needed to be done. So it is that photographs of Elle Macpherson from the Sports Illustrated swimsuit issue can be found in a portfolio that also includes the elderly Alfred M. Landon, who as the governor of Kansas ran unsuccessfully against President Franklin D. Roosevelt in 1936.
Mr. Lanker's debut as a documentary filmmaker came in 2000, on PBS, with "They Drew Fire: Combat Artists of World War II." He sought to preserve the work of artists who had been commissioned to paint scenes of warfare. Shortly before he died, Mr. Lanker whispered to Mr. Davaz, "There's just so much left to do."


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You might think which you can' t overcome depression, however, you may just need the right assistance. Depressive disorders is beatable when you know what to try and do. Below you will read many great tips that will show you what major depression is, and what you can do to defeat it.
Eating a balanced diet and making sure you take in the right amount of vitamins and nutrients is also essential to living a reliable and healthy life. Fruits and vegetables are an essential part of one' s diet, and what you eat has been proven to have got much to do with your mood.
A great tip that can help you fight major depression is to make sure you' re consuming a healthy diet. If you' re getting in poor nutrition, you might become exhausted and sluggish. This can lead to major depression. Eating healthy can go a long way in assisting you get out of your depression.
Depressive disorders is something we all have confronted at some point. If you are having bouts along with depression you should see a psychologist. Sometimes, they can identify a problem in your state of mind and work to reform it. In this way, the cause is treated and not simply the symptoms.
You can help major depression by working on any personal problems you have. Avoid getting overwhelmed getting into small things. Make your projects small so you can reach the goals.
Becoming interested in the arts is an excellent way to help you beat your major depression. If you like paintings or sculpture make sure to schedule lots of visits to local museums. Likewise if you like music make sure to visit as many concerts and displays as you are able to.
If you have been sensation down, uninterested in things that make you content, and are having trouble with sleep, this could be depression. Identifying depression is the initial step in solving it. If you have experienced these feelings for more than two weeks, it is necessary that you see a psychiatrist as soon as possible.
Try acupuncture. Acupuncture is an historic art that goes back many hundreds of years. In addition to the direct benefits of the acupuncture treatment, many acupuncture therapists use meditation and relaxation during the treatment. Together, they make for a very effective treatment for depression. You will leave your session renewed and ready to tackle living again.
When it comes to depression consider seeking the help of professionals in order to help you. This is important to consider because you cannot often control everything in your life and you may not be able to fix everything yourself. Consider visiting a professional in order to get your life straightened out.
To help with your depression, you need to form new relationships that are based on a support system that is there for you personally. As you find the support to be convenient and on-time, you will find that depression is definitely counteracted by a new approach that may greatly increase your confidence and show a person that you aren' t going on it alone.
In conclusion, many people have depression at some point. Understanding why you' re depressed is a large part of fighting it. With these tips, you can soon take your life back again.
3 Responses to "Excellent Advice To Help You Battle The Blues"
My boyfriend and I are moving in together in the next few weeks, and he has a 3 year old that has been placed on the Autism spectrum. The child's mother will have primary custody of her with us having her 2 days a week. I have absolutely no experience with children and I know this will be all the more difficult because of her Autism.
I have checked out several library books about Autism so I can learn more about it. The child has been in school since last October, in a class with 4 other children her age. She previously had issues with her attention span and her communication, but since she has been attending school, she is able to keep eye contact with you and is no longer as temperamental as she once was. Communication is still an issue, however. She does not talk. She can communicate by waving her arms and hands when she's happy or excited, and when she wants something she goes to find the object and gives it to you, but she doesn't say any words. The school has said that they will point to a color and repeat it back to her, and after 10 to 15 times, she eventually said 'blue', but I'm not sure if it's because she was just repeating it or because she can acknowledge the color blue.
This is a huge deal to me. Communication is something that's very important to me and I don't understand how I will be able to understand her needs. I know this is coming from someone that has no experience with kids though, so I'm thinking that the more I learn from her, the more I'll grasp the communication she does have.
I'm also uneasy that because we will only have her twice a week, how anything we try with her won't stick because she isn't with us that much. After a long time of battling with her mother, we've decided it's best for everyone for the child to stay with her. The woman is just not to my liking and in my opinion would sabotage anything I try to do to help this child, so I'm unsure about how to even come up with any sort of plan as for the extra time needed with the child's development. I'm fearful that even if the child greatly benefits from staying with us, the mother wouldn't adopt the same practices when the child goes back to her. (I know this last bit was bitter and probably too much info, and I know it raises the question as to why we don't have her full time, but the school is a good school and that's where the mother lives, and she makes life so miserable for us already that taking her kid away would literally be a nightmare. It's easier this way.)
I'm wondering if there's anyone out there that has some advice for me, and maybe some ideas about activities the child and I, and all three of us, can do together to help improve this issue. Anything is greatly appreciated.
Hello everyone,
I need a bit of advice/help with this quote in my book. In the frist book (Midnight Grey) the Great Bear spirit at the end says to the main character Skarlet, "…To the mountains that touch the sky and are home to the danceing colors…" Instructing her to go to the High Mountains and find the next Great Spirit. Now this is where I need a bit of help…in the second book (Surviving Shadows) she finds the Great Eagle spirit after a battle at the end. And after a bit of intence conversation between the two, Skarlet settles their fued over her destiny and the spirit says, "…To the waters that have no end and the deepest blue valleys…" Sending her on her way to the next Great Spirit…
Now my predicament is that I don't quite know if the last quote (The one I just typed for you) says enough. It is telling her to venture to the sea, but I don't want to say 'Sea' or 'Ocean'. Do you think that this is good enough to give the reader a bit to think about and leave Skarlet to think a little herself in the book? (She knows nothing of the sea or that such a thing even exists. Thus leading to a little bit of questions amoung friends in the third book.) If not then can you help me out a bit?
Thank you for your time.
P.S. Sorry for the miss spellings and grammar my Dylexia is a bit annoying… but really sorry about that…
I like what Morgan said, but sadly I can't use that. The book is almost Native American Indan, and the word type is very different then what most speak today. Sorry, but thank you for your input.
I wrote this story about 6 months ago for an English assignment – the assignment was for the story to be about a soldier at war (Could be science fiction to history, so long as it was about war). (I've already had my story graded, etc). I feel a bit funny about the ending; does anyone else feel like that?
I want your opinions on my story (i.e. they storyline, vocabulary, descriptions, ways to improve, how realistic it is, what era you think it was set in, whether you feel it'd be relevant now, whether you would read it etc.)
How mature is my writing (How old do you think I was when I wrote it)?
Please don't hold back, at all. LOL.
My story:
[Bold, Italics]Her eyes – those eyes, so beautiful; the deep blue sea that is hers, I am but another stranded sailor, the undulating waves so calming, cradling me to sleep . . . [Bold, Italics end]
The thunder of the explosion still ringing clear in my mind, I continue forwards, running, stumbling, marching towards [Italics]them[Italics end]. I am still clueless on how I evaded the dynamite, remembering only that I fell . . . and the metallic taste of blood in my mouth, upon impact, bearing witness to that fact.
The dry mud made my trousers cling onto my legs; everything feels so heavy now, it's almost impossible to progress further. How can the Sun shine at a time like this? With the world in ruin, death and destruction in every corner, she shines, fiercer than ever.
The Sun continued to glare down upon us, as if in a disapproving manner. The glorious Sun, a thousand million years old, seen too much of the same suffering for it to affect her; beaming onto her child, as if she was oblivious. She, a magnificent ball of fire in the sky; uplifting for just a brief moment, but excruciating for the rest.
I want to stop. I [Italics]have[Italics end] to stop. [ItalicsI have to stop[Italics end]. My feet cannot carry me a step further; the soles of my boots worn away, and with them the only comfort I found in this minefield. My throbbing feet, so raw, stinging with every advance – perhaps this is a perfect portrayal of my tattered conscience.
Wiping away the droplets of perspiration that have formed on my forehead, for what seems like the hundredth time, I persist onwards. I feel like I have no control of my body, my mind now the minefield. [Italics]"FORWARD MARCH, TROOPS!"[Italics end] a memory, which I cannot place, being the only conscious thought in my head.
War, at the eleventh hour, is a great hallucinogen; I know not if this is what I am experiencing, nor if this is true, but at last I feel free. The conscience of a murderer is never clean; it is darkened with each thought, each glimpse of every victim, even more so for a murder that is forced. Every second that is spent anticipating a death is another morbid memory for a murderer; it is possible they suffer more than the kill, but that is apparently narcissistic of me to say. This is what I am, a soldier: a glorified name for a murderer, a killer of people.
I see things now, everything is so much clearer; like they said, battle [Italics]is[Italics end] a good place to be. The ground so soft, a thick, liquid-like substance seeping through the gaps of my toes, taking me back to Autumn days, and barefoot racing; memories of a childhood, not so long ago. Distorted sounds behind me echo in my mind, whilst the vision of a blinding light, flashing uncertainly, overtakes my sight. I am moving at an escalating speed now, an unknown power taking me further and further, lifting me until I feel like I am flying – no, no – I [Italics]am[Italics end] flying! Drunk on euphoria – never have I felt something so great. Suddenly I am crashing, hurtling back down to reality. I must say, heaven short-lived is worthy of a thousand lifetimes. In a sharp second, it is all over.
I feel a sweeping blaze in my stomach, from an ember into a fierce burning, I cannot hold it. It continues within me, devouring me whole, until I cannot breathe. I feel the air passing through my mouth, as I am now gasping, but no further – I am choking. I clutch at my chest, willing myself to breathe. There is an unidentifiable force grabbing my core, constricting its natural beat, until it trembles to a halt; with the death of a drum, dies the lone dancer.
They say at the time of death angels surround you, singing hymns, and softly raise you into a glowing white light. I, however, was met with darkness – pitch black. No angels comforted me, as a searing hot flash penetrated my outer layer, into my innards, bringing with it pain of the worst physical kind. No angels came to my aid, as I lay writhing on the battlefield, pleading for help. But, what did I expect, a murderer like me?
For me, there is no light. My only peace is in knowing I have an eternity to spend with my Rose, the only [Italics]angel[Italics end] I'll ever need.
[Bold, Italics]She is my angel, her laugh, a symphony, to which no other can com
[Story cont.] [Bold, Italics]She is my angel, her laugh, a symphony, to which no other can compare, her caress, so tender, so soft. She is my angel, calling me to our heaven.[Bold, Italics end]
Thanks for taking the time to read it!


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|Table of Contents
|Vol. 38, No. 2, 2001
Issue release date: March–April 2001
|Section title: Research Paper
Differential Effects of Vascular Endothelial Growth Factor-C and Placental Growth Factor-1 on the Hydraulic Conductivity of Frog Mesenteric CapillariesHillman N.J.a · Whittles C.E.b · Pocock T.M.b · Williams B.a · Bates D.O.b
aCardiovascular Research Institute, University of Leicester, Leicester, bDepartment of Physiology, University of Bristol, Bristol, UK Corresponding Author
Vascular endothelial growth factors (VEGFs) are known to increase vascular permeability. VEGF-A acts on two receptor tyrosine kinases, VEGF receptor-1 (VEGF-R1 or flt-1) and VEGF receptor-2 (VEGF-R2, flk-1 or KDR). VEGF-C acts only on VEGF-R2 on vascular endothelial cells, whereas placental growth factor-1 (PlGF-1) acts only on VEGF-R1. The effects of perfusion of these receptor-specific proteins on hydraulic conductivity (Lp) was measured in frog mesenteric capillaries. The effect of PlGF on Lp was not conclusive, and overall fluid flux did not increase during that time. VEGF-C acutely and transiently increased Lp (4.5 ± 0.9-fold), which was more obvious in a subset of vessels, in a similar manner to that reported for VEGF-A. In the subset of vessels in which VEGF-C significantly increased Lp acutely, there was a sustained 12-fold increase in Lp 20 min after perfusion, but this was not seen in those vessels which did not respond acutely to VEGF-C, or in vessels exposed to PlGF-1. Lp was also increased 24 h after perfusion with VEGF-C, but not with PlGF-1. Western blot analysis showed that VEGF-R1 and VEGF-R2 are both present in frog tissue. These data show that the VEGFs that stimulate VEGF-R2 chronically increase Lp, but not those that stimulate VEGF-R1 only. This supports the hypothesis that chronic increases in microvascular permeability induced by VEGF are mediated via activation of VEGF-R2 rather than VEGF-R1.
© 2001 S. Karger AG, Basel
Vascular endothelial growth factors (VEGFs) are a family of endothelial cell-specific peptides that act to increase the delivery of nutrients to tissue by increasing the rate of angiogenesis, stimulating vasodilatation and increasing microvascular permeability [1]. VEGF production is associated with a number of normal physiological processes including wound healing and the female reproductive cycle [2], and VEGFs have been implicated in the pathogenesis of a variety of vascular diseases including tumour growth [3], myocardial infarction [4], hypertension [5] and diabetes [6]. VEGFs are currently being investigated as treatment for coronary and peripheral ischaemic disease [7]. VEGF-A is the most common secreted form of VEGF and is currently being used in clinical trials for revascularisation [8]. Previous studies have shown that the perfusion of microvessels in vivo with VEGF-A causes an acute, transient increase in microvascular hydraulic conductivity (Lp) [9] and diffusive permeability to albumin [10]. This acute increase is followed by a chronic, sustained increase in Lp after 24 h, but no increase in mean pore size, since there is not a reduction in the oncotic reflection coefficient to albumin [11]. Despite the undoubted importance of VEGF in a variety of pathologies, and the likely use of VEGF in clinical medicine in the future, very little is known about the mechanisms which underlie either the acute or chronic increase in permeability mediated by VEGF in microvessels in vivo.
VEGF-A is known to act principally via two receptor tyrosine kinases in vascular endothelial cells, i.e. VEGF receptor-1 (VEGF-R1 or flt-1) and VEGF receptor-2 (VEGF-R2, flk-1 or KDR) [3]. The aim of this study was to determine the separate roles and importance of the two receptors in the initiation of both the acute and chronic permeability increases caused by VEGF. We have therefore used receptor-specific endogenous agonists. Placental growth factor-1 (PlGF-1) acts only on VEGF-R1 [12], and VEGF-C acts only on VEGF-R2 in vascular endothelial cells [13]. We have studied the separate effects of these reagents on microvascular permeability. This work has been presented in abstract form to the Physiological Society [14, 15].
materials and methods
All experiments were carried out on adult male frogs (Rana temporaria) supplied by Blades, UK. All chemicals were purchased from Sigma unless otherwise specified. The frogs were anaesthetised by immersion in 0.1% 3-aminobenzoic acid ethyl ester (MS222) in tap water. The animal was laid supine and the limbs were lightly secured. A small incision (8–10 mm) was made in the right lateral skin and body wall, and the distal ileum was exteriorised and draped over a transparent quartz pillar 1 cm in diameter. The upper surface of the mesentery was kept continuously superfused with frog Ringer's solution, pH 7.4 [in mM: 111 NaCl, 2.4 KCl, 1 MgSO4, 1.1 CaCl2, 0.2 NaHCO3, 2.63 N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic (HEPES) acid and 2.37 HEPES sodium salt], containing 0.02% MS222 at room temperature (20–22°C). The capillaries in the mesentery were visualised using a microscope and videoed through an electronic timer for off-line analysis.
The Lp of isolated mesenteric capillaries was measured using the Landis micro-occlusion method [16] modified as described previously [9]. Baseline Lp was defined as the mean Lp measured during 3–5 micro-occlusions while perfusing with 1% bovine serum albumin (BSA; Sigma, A4378) in Ringer's solution (adjusted to pH 7.4 with 0.115 mM NaOH) prior to perfusion with reagent. Microvessels were chosen that had divergent flow at the start of the vessel and convergent flow at the end (defined as capillaries). They were suitable if they had brisk flow with no white cells adhering or rolling along the wall, were at least 800 μm long and 15–25 μm in diameter with no side branches, and had a baseline Lp of <10 × 10–7 cm·s–1·cm H2O–1 [9]. Vessels with a baseline Lp greater than 10 × 10–7 cm·s–1· cm H2O–1 were excluded. Each vessel was cannulated with a glass micropipette made from a pulled and bevelled glass capillary tube. The micropipette was filled with 1% BSA in frog Ringer's solution with rat red blood cells as flow markers. Rat red cells were collected by direct cardiac puncture of 5% halothane-anaesthetised rats. The rats were then killed by cervical dislocation. The rat red blood cells were washed three times in frog Ringer's solution before use. The filled micropipette was clamped in a holder that was connected to a water manometer to control the perfusion pressure. The capillary was perfused with the solution and occluded with a glass rod downstream of the cannulation site. The occlusion was maintained for 3–7 s, then the glass rod was removed and free flow was allowed for a few seconds before occluding again. The Lp of the capillary was determined from the filtration rate per unit area, calculated as described below, and the hydrostatic and oncotic pressures across the capillary wall, according to Starling's hypothesis [17].
The rate of filtration per unit area of capillary wall (Jv/S) was calculated from the initial velocity of the flow marker red blood cells (dl/dt) after occlusion of the microvessel and the surface area of the vessel available for filtration (S). S was calculated from the internal radius of the capillary (r) and the length between the marker cell and the point of occlusion (l). Thus:
Jv/S = (dl/dt)(r/2l)
All of these measurements were made off-line from a videotape recording of the whole experiment. The Lp was calculated from the Starling equation:
Lp = (Jv/S)/ΔP
where ΔP is the effective hydrostatic and oncotic pressure difference between the capillary and the surrounding tissue. As 1% BSA has an effective oncotic pressure of 3.6 cm H2O, the pressure used to calculate Lp was 3.6 cm H2O lower than the pressure set in the manometer, assuming that tissue hydrostatic pressure was negligible and tissue oncotic pressure was equivalent to that in the superfusate (zero).
Following baseline Lp measurement in a single microvessel, the micropipette was refilled with Ringer's solution containing 1% BSA, marker red cells and reagent. A refilling system based on that described by Neal [18] was used. The refilling system consisted of two syringes on an electric push-pull pump (SP120, WPI, Stevenage). The pushing syringe (5 ml) was connected to flexible polyethylene tube (PE50, Portex) that was connected to hand-drawn PE50 tubing glued into a male-male Luer connector. The drawn part was fed through the micropipette holder and up to the shoulder of the micropipette. The pulling syringe (20 ml) was connected to a similar tube that led through the micropipette holder to the back of the micropipette. Both syringes were attached to the tubes via taps that allowed the syringes to be closed off from the tubes and therefore from the micropipette (fig. 1). Before use, the tubes were filled with 1% BSA in frog Ringer's solution, expelling all air bubbles, and were closed off from the syringes. After cannulation, the pipette was refilled with 1% BSA containing a low haematocrit (1–10%) of red blood cells. Following baseline Lp measurement, the pushing syringe was filled with a solution of 1% BSA in frog Ringer's solution, with rat red blood cells and either 10 nM VEGF-C (a kind gift from Prof. K. Alitalo) or 10 nM PlGF-1 (a kind gift from Dr. M.G. Persico, or from R&D Systems). Both syringes were opened to the tubes and micropipette, and the pump was activated. The solution containing reagent therefore filled the front of the micropipette and the baseline solution was removed from the back of the micropipette. Switching of the solution was complete within 7 s (as determined both by filling with dye or by the change in haematocrit, pumping at maximum rate and switching 0.75 ml). As soon as the refilling was complete, the syringes were isolated from the micropipette by closing the taps to allow pressure equilibration, and the vessel was occluded as soon as possible to allow Lp measurement. In order to determine whether the system refilled without raising the pressure in the pipette, control experiments were carried out by refilling with 1% BSA. The filtration rate did not increase when measured at intervals during refilling. Lp was measured approximately every 15 s over the next 5 min.
Fig. 1. Diagram of the refilling system. The new agonist to be perfused is placed in a 5-ml infusion syringe (a). This is connected to a 20-ml withdrawal syringe (b), on a push-pull electronic pump (c). When the pipette is to be refilled, the pump drives 0.75 ml of solution through the filling line (d) into the tip of the pipette (e), and provides suction to the back of the pipette through the suction line (f). Once the solution has filled the pipette, the taps to the syringes (g, h) are closed, and the pressure equilibrates with that of the water manometer connected to the back of the pipette (i).
The effect on microvascular permeability 20 min after perfusion with reagent was determined in 13 vessels perfused with 10 nM VEGF-C and 8 vessels perfused with 10 nM PlGF-1. Following the Lp measurements described above, the micropipette was refilled with 1% BSA in frog Ringer's solution with no rat blood cells and the vessel was perfused at 30 cm H2O for 20 min. The micropipette was then refilled with 1% BSA in frog Ringer's solution with rat red blood cells and further Lp measurements were made.
The effect on microvascular permeability 24 h after perfusion with reagent was determined in 8 vessels perfused with 10 nM VEGF-C and 8 vessels perfused with 10 nM PlGF-1. Following the Lp measurements described above, a map of the mesentery was drawn in order that the same vessel could be located 24 h later. The frog gut mesentery consists of panels of connective tissue bordered by arteries and veins. The characteristic spacing of the arteries and veins allows an individual panel to be easily located from the map. All the visible microvessels in the connective tissue panel and the location of the arteries and veins crossing the mesentery were drawn. The location of the relevant panel of connective tissue in relation to the ileo-caecal junction was also noted and the cannulated vessel was marked on the map. The gut was then replaced in the body cavity and the body wall and skin were sutured. The animal was untied, allowed to recover whilst partially immersed in water, and then kept at room temperature. After 24 h, the frog was anaesthetised and the mesentery exposed as before. The same panel of connective tissue was found from the vascular architecture and its position relative to the ileo-caecal junction. The experimental capillary was located in the panel from the map drawn on day 1. The vessel was cannulated downstream of the cannulation site from the previous day, and blocked upstream of the block site from the previous day. Permeability was therefore measured on an undamaged section of the same vessel, which also formed the majority of the vessel investigated the previous day. Baseline Lp (perfusion with 1% BSA in frog Ringer's solution with rat red blood cells) was measured as before. The criteria for acceptable Lp measurement on day 2 were similar to that of day 1, in that the capillary must have contained mobile red blood cells and no white blood cells adhering to the vessel wall. However, on day 2, there was no maximum Lp over which the vessel would be discarded, and the minimum available length of the capillary was only 400 μm.
To control for possible artefacts of anaesthetic, surgery or cannulation, Lp was measured in a group of vessels in exactly the same way as described above (15 vessels studied acutely, 5 after 20 min and 8 after 24 h). However, in each case, the pipette was always refilled with 1% BSA in frog Ringer's solution with rat red blood cells, and never with either PlGF-1 or VEGF-C.
For analyses of the time course of the acute response to growth factors, the data were combined by grouping each measurement of Lp, or Lp relative to baseline, into 15-second intervals for each microvessel. The mean and standard error of the mean (SEM) were then calculated for each 15-second group. The peak increase in Lp reported in the results section is the mean of the individual maximum measured, not the peak value of the mean responses, and will therefore be greater than the time-averaged response seen in the figures. Relative changes (fold increases) in Lp were not normally distributed, and therefore non-parametric statistics (including median ± interquartile ranges) were used to analyse these changes. A value of p < 0.05 was considered significant.
In order to ensure that VEGF-R1 and VEGF-R2 were present in frog vessels, various frog tissues were processed for Western blot analysis with antibodies to human VEGF-R1 and VEGF-R2. Frog tissues were collected from animals pithed by cervical dislocation and destruction of the brain. Parasites were removed from the lung if present, and the tissue was then gently homogenised in 100 μl of phosphate-buffered saline (PBS) containing protease inhibitors: 0.2 μg·ml–1 leupeptin, 0.2 μg·ml–1 pepstatin A, 80 μg·ml–1 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride and 0.1 mg/ml ethyldiaminotetraacetic acid, sodium salt. Protein was then extracted by resuspending the homogenate in an equal volume of extraction buffer (300 mM NaCl, 20 mM Tris, 10 mM EDTA) containing 2% Triton X-100, 0.2% sodium dodecyl sulphate (SDS), 2 mM sodium orthovanadate (Na3VO4) and 1 mM phenyl methyl sulphonyl fluoride. Homogenates were spun in a benchtop centrifuge for 10 min at 10,000 g at 4°C to separate the protein and cell debris, and 10-μl aliquots were removed to determine the protein concentration by the Bradford method (BioRad). For each sample, 105 μg (except the heart tissue for the VEGF-R2 blot, where 20 μg was used) of protein was used for SDS polyacrylamide gel electrophoresis. The gel was electroblotted onto a polyvinylidine difluoride membrane at 250 mA for 3 h. The membrane was washed in 1 × PBS-0.1% Tween (PBS-T) then blocked in 10% marvel in PBS-T for 2 h, and probed with a mouse anti-VEGF-R2 antibody (Santa Cruz, SC-6251) or a goat anti-VEGF-R1 antibody (Santa Cruz, SC316-G) at 1:1,000 (VEGF-R2) or 1:300 (VEGF-R1) in 5% marvel. The membrane was washed in PBS-T, incubated with HRP-conjugated anti-goat (Santa Cruz, 1:3,000) or anti-mouse (Dako, 1:1,000) secondary antibody and then washed 5 times for 5 min in PBS-T (anti-VEGF-R2) or 3 times for 1 h in PBS-T (anti-VEGF-R1). Protein was detected with enhanced chemiluminescence Western blotting detection reagent (Roche, UK) and exposed to photo-sensitive film for 1 min and developed. Bands present were measured against coloured markers (BioRad) also loaded onto the gel. Figures are representative of three gels run for each antibody.
Results from Western blot analysis showed that both VEGF-R1 and VEGF-R2 are present in frog tissues using antibodies to the human receptors. Bands were seen in the lung, kidney, liver and heart, which have previously been shown to be sites for expression in mammalian systems, and importantly, both were also present in the mesentery (fig. 2).
Fig. 2. Western blot of protein extracted from various frog tissues. Protein samples were equally loaded (105 μg), except for heart on the VEGF-R2 blot (20 μg). a Blot probed using antibody to VEGF-R1. Bands were seen at approximately 200 and 150 kD, consistent with glycosylated and pre-glycosylated mammalian forms in frog liver, kidney, heart, lung and mesentery. b Blot probed using antibody to VEGF-R2. Bands were seen at 150 kD, consistent with mammalian VEGF-R2 in frog heart, lung, kidney and mesentery, but not in liver.
Baseline Lp was defined as the Lp during perfusion with 1% BSA in frog Ringer's solution prior to perfusion with reagent. For each vessel, 5 or 6 baseline readings were taken and averaged. This value was the baseline Lp for that vessel and was taken as time point zero. Vessels were then perfused with reagent and Lp measurements were taken over a 5-min period. Perfusion of 34 vessels with 10 nM VEGF-C caused an acute and transient median 2.5 ± 0.8-fold increase in Lp [from a baseline Lp (mean ± SEM) of 3.5 ± 0.5 to a peak of 13.0 ± 3.2 × 10–7 cm·s–1· cm H2O–1; p < 0.001, Wilcoxon paired test (WPT)]. Lp returned to baseline values during the 5-min perfusion with VEGF-C (to 5.1 ± 1.2 × 10–7 cm·s–1·cm H2O–1). For comparison of the time course of the response, Lp measurements during 5 min (300 s) of perfusion with VEGF-C for each vessel were grouped into 15-second bins and the mean Lp for each time point was calculated (fig. 3). Lp was significantly increased compared to the baseline value (point zero) during 12 of the first 13 15-second time points (up to 200 s), after which, the Lp was not significantly greater than the baseline value. If one chooses even stricter criteria for a response, such as those described in a previous report (i.e. the peak reading during perfusion with reagent is greater than 2 times the standard deviation of the mean baseline readings of that entire group of vessels [9]), which yields only 44% of the vessels being responders, these vessels behaved like the whole group. Since a heterogeneous population of vessels was being investigated, we separated the results from the vessels that showed a response (responders), from those that did not (non-responders). The time-averaged data for the two groups is shown in figure 3b. Again, during the 5-min perfusion period, there was a significant increase in Lp compared to baseline for the first 200 s for responding vessels, after which Lp returned to baseline values.
Fig. 3. The acute effect of VEGF-C on Lp (actual values). a Time-averaged Lp measurements (15-second bins over 5 min) from 34 vessels perfused with 10 nM VEGF-C (○) or 1% BSA (■). b Time-averaged Lp measurements (15-second bins over 5 min) of vessels categorised as to whether the maximal Lp reading during perfusion with reagent was more than 2 standard deviations greater than the mean baseline Lp [n = 15, responders (○)] or not [n = 19, non-responders (▲)].
The maximal value of Lp during perfusion of 19 vessels with 10 nM PlGF-1 was significantly greater than the baseline value (10.9 ± 3.0 compared to 3.4 ± 0.6 × 10–7 cm·s–1·cm H2O–1; p < 0.05, WPT). However, there were no consistent time points at which the maximum Lp measurement occurred, and the mean Lp at any particular time point after the start of perfusion was not significantly greater than the average baseline value. Neither was Lp significantly different from baseline values after 5 min (6.5 ± 2.3 × 10–7 cm·s–1·cm H2O–1). This can be seen statistically, since none of the time-averaged data (fig. 4a) showed a significant increase in Lp. To determine whether there was a significant increase in water flux during VEGF perfusion, the mean area under the permeability-time curve was calculated. (For each vessel, the mean baseline Lp for that vessel prior to perfusion with PlGF-1 was subtracted from each Lp reading during PlGF-1 perfusion and the results were plotted. The area under this curve was calculated and the mean of the group of vessels was taken.) Comparison of the mean area under the curve for PlGF-1 perfusion (all vessels: 742 ± 512 cm·cm H2O–1) with the mean area under the curve for 1% BSA perfusion (–102 ± 63 cm·cm H2O–1) over the same time interval showed that the increase in Lp during PlGF-1 perfusion was not statistically significant (p = 0.45, Mann-Whitney U test). Therefore, in these experiments, the effects of PlGF were inconclusive, in contrast to those of VEGF-C and VEGF-A.
Fig. 4. The acute effect of PlGF-1 on Lp (actual values). a Time-averaged Lp measurements (15-second bins over 5 min) from 19 vessels perfused with 10 nM PlGF-1 (○) or 1% BSA (■). b Time-averaged Lp measurements (15-second bins over 5 min) of vessels categorised as to whether the peak Lp reading during perfusion with reagent was more than 2 standard deviations greater than the mean baseline Lp [n = 5, responders (●)] or not [n = 14, non-responders (△)].
Perfusion with 1% BSA in frog Ringer's solution with rat red blood cells and no reagent did not significantly alter microvascular permeability (15 vessels; increase from a baseline value of 2.3 ± 0.4 to a peak of 3.2 ± 0.5 × 10–7 cm·s–1·cm H2O–1; p = 0.19, WPT).
Lp was measured in 13 vessels 20 min after perfusion with VEGF-C and in 8 vessels 20 min after perfusion with PlGF-1. Lp was significantly increased in vessels perfused with VEGF-C 7.4 ± 2.9-fold after 20 min (from 3.4 ± 0.6 to 48.2 ± 20 × 10–7 cm·s–1·cm H2O–1; p < 0.05, WPT). In contrast, there was no significant increase in Lp in vessels perfused with PlGF-1 after 20 min (4.6 ± 0.7 compared to 5.8 ± 1.2 × 10–7 cm·s–1·cm H2O–1; p = 0.38, WPT) (fig. 5).
Fig. 5. The acute and chronic effect of growth factors (relative to baseline on day 1). VEGF-C (10 nM; n = 8, solid bars), PlGF-1 (10 nM; n = 8, grey bars) or 1% BSA alone (n = 8, open bars) were perfused into vessels and Lp was measured acutely, 20 min after perfusion and 24 h after perfusion. Values are medians ± interquartile ranges.
In order to determine whether the raised Lp 20 min after perfusion with 10 nM VEGF-C was linked to the initial response, responsive and unresponsive vessels were analysed separately. In responsive vessels (n = 7), the baseline value was 4.1 ± 1.0 × 10–7 cm·s–1·cm H2O–1, which increased 12.2 ± 5.9-fold after 20 min to 84.8 ± 31.4 × 10–7 cm·s–1·cm H2O–1 (p < 0.05, WPT), but in the unresponsive vessels (n = 6), there was a small and insignificant change from a baseline value of 2.6 ± 0.6 to a value of 5.4 ± 4.0 × 10–7 cm·s–1·cm H2O–1 (p = 0.51, WPT) after 20 min. A significant increase in Lp 20 min after perfusion with VEGF-C was therefore only seen in vessels that had responded acutely to VEGF-C (fig. 6). There was also no effect of 1% BSA on Lp after 20 min (n = 5; change from baseline value of 2.3 ± 0.8 to 10.5 ± 4.3 × 10–7 cm·s–1·cm H2O–1 after 20 min; p = 0.13, WPT). These results suggest that the increase in Lp after 20 min only occurs in vessels that show an acute Lp increase in response to VEGF-C, and that PlGF-1 does not increase Lp over a longer time period.
Fig. 6. The acute and 20-min increases in Lp are linked (values shown are relative to baseline). The median peak Lp is shown for vessels perfused with 10 nM VEGF-C (n = 13) or PlGF-1 (n = 8), measured during (grey bars) or 20 min after (solid bars) growth factor perfusion. Vessels in which Lp was acutely increased during VEGF-C perfusion had a significantly increased Lp 20 min later. This was not true for vessels that did not show acutely increased permeability, nor was it true for any vessels perfused with PlGF-1. Values are medians ± interquartile ranges. * p < 0.05.
Lp was significantly increased 7.6 ± 3.0-fold in 8 vessels 24 h after perfusion with VEGF-C, from 2.7 ± 1.1 to 19.6 ± 8.0 × 10–7 cm·s–1·cm H2O–1 (p < 0.05, WPT). In most of the vessels, no overt angiogenesis was seen, although in one vessel (data not included), a new side branch was clearly seen, which made Lp measurement impossible. In contrast, there was no increase in Lp in 8 vessels 24 h after perfusion with PlGF-1 (from a baseline value of 2.7 ± 1.0 to 5.2 ± 1.3 × 10–7 cm·s–1·cm H2O–1 24 h later; p = 0.11, WPT; fig. 5). Further, 1% BSA perfusion did not result in an increase in permeability the following day either (n = 8; 2.5 ± 0.6 to 3.7 ± 1.3 × 10–7 cm·s–1·cm H2O–1). This was consistent with previously published data on the chronic effect of perfusion of single vessels with 1% BSA [9]. There was no relationship between the increase in Lp on the first day and the size of the Lp on the second day in any of the vessels perfused (with VEGF-C, PlGF-1 or 1% BSA; fig. 7). However, there was a significant relation between the acute and the 20-min Lp for VEGF-C, but not for PlGF-1 or 1% BSA, confirming that the acute increase in Lp is somehow linked to the more sustained increase.
Fig. 7. The relationship between the acute and chronic changes in permeability. a There was no relationship between the acute change in permeability and the Lp 24 h later for any of the groups studied. b There was a significant correlation (r = 0.769, p < 0.02) between the magnitude of the acute response and the response 20 min later in vessels perfused with VEGF-C (solid regression line). There was no relationship between the Lp during PlGF-1 (r = 0.181; dotted line) or BSA (r = –0.186; dashed line) perfusion and the Lp 20 min later.
The diameters of the vessels perfused with VEGF-C or PlGF-1 were measured on both days. Although there was no change in diameter in vessels perfused with PlGF-1, VEGF-C perfusion, in contrast, resulted in a significant increase in vessel diameter, from 20.5 ± 1.4 to 25.2 ± 2.0 μm (n = 8; p < 0.05, paired t test). This increase is similar to that described for VEGF-A [9] (fig. 8).
Fig. 8. VEGF-C but not PlGF-1 increased capillary diameter. The diameter of capillaries was measured before (grey bars) and 24 h after (solid bars) perfusion with 10 nM VEGF-C or PlGF-1. The diameter was significantly greater 24 h after VEGF-C perfusion (* p < 0.02, paired t test), but no different in PlGF-1-perfused vessels. There was no relationship between the diameter of the vessels and any of the permeability measurements.
VEGFs were originally isolated according to their ability to increase the extravasation of macromolecules [19], over 10 years ago. However, it was a further 6 years before the permeability-enhancing effects of VEGF were described in single perfused microvessels [9]. Despite the importance of this family of growth factors in the generation of leaky vessels, pathological oedema and tumour-related effusions, the mechanisms through which VEGFs act have still not been clearly elucidated [1]. What has been shown is that VEGF-A, the most common isoform of VEGF, can cause an acute, transient increase in Lp [9] and albumin permeability in vivo [10] that is followed by a chronic, sustained increase in Lp after 24 h [9]. VEGF has been shown in frogs in vivo to acutely increase permeability through a pathway that is dependent on calcium influx [20] and independent of calcium store release [21]. This pathway has been shown in pig coronary vessels to be dependent on nitric oxide formation [10]. There is evidence that this may be through phospholipase C (PLC) activation [21, 22], and activation of protein kinase C. Although there is now some evidence that PLC is specifically activated by VEGF-R2 and not VEGF-R1 [23], it is not known which of the two VEGF receptors is responsible for one of the functional end points of VEGF stimulation – increased permeability. The results described here show that VEGF-C can cause an acute, transient increase in microvascular permeability, and can also cause the sustained, more chronic increase in Lp. VEGF-C has been known to stimulate only VEGF-R2 in previously studied tissues, whereas PlGF-1 can only stimulate VEGF-R1 [3]. This suggests that the chronic increase was mediated via VEGF-R2 and not VEGF-R1. Although it is possible that the PlGF was not given at a high enough concentration to stimulate VEGF-R1, this is unlikely, since the affinity of VEGF-R1 is higher for PlGF (230 pM) [24] than that of VEGF-R2 for VEGF-C (410 pM) [25]. In addition, we were using doses nearly two orders of magnitude higher than the affinities of the receptors for their ligands. However, further interpretation should be cautious in the absence of a dose response curve for both VEGF-C and PlGF. Since it has previously been shown that VEGF-R1 stimulation does not result in PLC=γ activation [26], but that VEGF-R2 activation does [27], then these data are consistent with the hypothesis that VEGF acts to increase permeability through VEGF-R2-mediated activation of PLC=γ [23]. Some vessels studied failed to show a change in Lp in response to VEGF-C perfusion. There was no visible difference between these vessels and responsive vessels, and it was impossible to predict in advance whether vessels would show a response or not. It may be that unresponsive vessels do not express VEGF-R2, or that the receptor is present but inactive.
VEGF-A acts either as a homodimer or as a heterodimer with other members of the VEGF family. VEGF-A has two separate receptor-binding domains specific to VEGF-R1 or VEGF-R2. Due to the arrangement of these binding sites, the VEGF dimer can bind two receptors, either as homo- or heterodimers [3]. PlGF has been shown to down-regulate the angiogenic effect of VEGF-A [28], and this may be due to stimulated VEGF-R1 attenuating the effect of stimulation of VEGF-R2. We have not studied the effects of heterodimerisation; we perfused capillaries with only one receptor-specific reagent at a time. However, our results with VEGF-C showed a chronic increase in Lp after 20 min, which is not seen with VEGF-A. There is also a more marked increase in Lp after 24 h with VEGF-C compared to previous results with VEGF-A [9]. This suggests that perfusion of microvessels with VEGF-A may result in the stimulation of both receptors, and VEGF-R1 may attenuate the effect of VEGF-R2, resulting in a lesser effect on permeability than stimulation of VEGF-R2 alone.
It has previously been shown that perfusion of microvessels with VEGF-A increased the diameters of vessels after 24 h [9]. In this study, this effect was also seen in vessels perfused with VEGF-C, but not in vessels perfused with PlGF-1. An increase in diameter has been shown to be dependent on mitogen-activated protein kinase (MAPK) activation [29], and both PlGF-1 and VEGF-C have been shown to activate MAPK [26, 30]. One hypothesis is that there was no VEGF-R1 expression on the mesenteric capillaries (unlikely since there was VEGF-R1 expression in the mesentery, which consists of connective tissue, mesothelial cells and blood vessels). Alternatively, it may be that the rest of the signalling pathway necessary to stimulate the increase in diameter is not stimulated by VEGF-R1.
In summary, our data show that VEGF-C, but not PlGF-1, increases the Lp of perfused mesenteric capillaries after 20 min and 24 h. Although VEGF-C clearly and consistently results in an acute increase in Lp lasting a few minutes, the acute effects of PlGF-1 were inconclusive. These data are consistent with the hypothesis that the previously described acute and chronic increases in Lp in frog mesenteric microvessels are stimulated by VEGF activation of VEGF-R2. In addition, the increase in diameter brought about by VEGF is also seen when VEGF-C is used to stimulate vessels, but not when PlGF-1 is used, and therefore these data are consistent with the hypothesis that the diameter increase is also brought about by VEGF-R2 activation.
This study was supported by the Wellcome Trust (No. 050742 to N.J.H.), the British Heart Foundation (PG97198 to T.M.P., FS98023 to D.O.B.) and the National Kidney Research Foundation (R35/1/98 to C.E.W.).
Dr. David Bates
Department of Physiology, The University of Bristol, The Vet School
Southwell Street, Bristol BS2 8EJ (UK)
Tel. +44 117 928 7823, Fax +44 117 925 4794
E-Mail email@example.com
Received: Received: April 14, 2000
Accepted after revision: November 9, 2000
Number of Print Pages : 11
Number of Figures : 8, Number of Tables : 0, Number of References : 32
Journal of Vascular Research
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)
Vol. 38, No. 2, Year 2001 (Cover Date: March-April 2001)
Journal Editor: M.J. Mulvany, Aarhus
ISSN: 1018–1172 (print), 1423–0135 (Online)
For additional information: http://www.karger.com/journals/jvr
Vascular endothelial growth factors (VEGFs) are known to increase vascular permeability. VEGF-A acts on two receptor tyrosine kinases, VEGF receptor-1 (VEGF-R1 or flt-1) and VEGF receptor-2 (VEGF-R2, flk-1 or KDR). VEGF-C acts only on VEGF-R2 on vascular endothelial cells, whereas placental growth factor-1 (PlGF-1) acts only on VEGF-R1. The effects of perfusion of these receptor-specific proteins on hydraulic conductivity (Lp) was measured in frog mesenteric capillaries. The effect of PlGF on Lp was not conclusive, and overall fluid flux did not increase during that time. VEGF-C acutely and transiently increased Lp (4.5 ± 0.9-fold), which was more obvious in a subset of vessels, in a similar manner to that reported for VEGF-A. In the subset of vessels in which VEGF-C significantly increased Lp acutely, there was a sustained 12-fold increase in Lp 20 min after perfusion, but this was not seen in those vessels which did not respond acutely to VEGF-C, or in vessels exposed to PlGF-1. Lp was also increased 24 h after perfusion with VEGF-C, but not with PlGF-1. Western blot analysis showed that VEGF-R1 and VEGF-R2 are both present in frog tissue. These data show that the VEGFs that stimulate VEGF-R2 chronically increase Lp, but not those that stimulate VEGF-R1 only. This supports the hypothesis that chronic increases in microvascular permeability induced by VEGF are mediated via activation of VEGF-R2 rather than VEGF-R1.
© 2001 S. Karger AG, Basel
Dr. David Bates
Department of Physiology, The University of Bristol, The Vet School
Southwell Street, Bristol BS2 8EJ (UK)
Tel. +44 117 928 7823, Fax +44 117 925 4794
E-Mail email@example.com
Received: Received: April 14, 2000
Accepted after revision: November 9, 2000
Number of Print Pages : 11
Number of Figures : 8, Number of Tables : 0, Number of References : 32
Journal of Vascular Research
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)
Vol. 38, No. 2, Year 2001 (Cover Date: March-April 2001)
Journal Editor: M.J. Mulvany, Aarhus
ISSN: 1018–1172 (print), 1423–0135 (Online)
For additional information: http://www.karger.com/journals/jvr
Published online: 4/13/2001
Issue release date: March–April 2001
Number of Print Pages: 11
Number of Figures: 8
Number of Tables: 0
ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)
For additional information: http://www.karger.com/JVR


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Love is complicated and confusing, that's what! But it doesn't have to be. Save 50% off an 11-Card Love Celtic Cross Tarot reading now for answers to all your love questions!
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Wheel of Fortune
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Read the full story at http://www.prweb.com/releases/2013/8/prweb11065130.htm.
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My roles as a pre-press operator included creating artwork approvals for customers (such as Lush Cosmetics and English Bay Batter), and preparing files for plate making and digital output
My roles included: managing online presence (social media, blog, marketing, etc), website redesign, branding collateral design (business cards, postcards, brochures, etc), photography, and videography
My roles at Blender include creative direction, identity development, and web & print design for publicly traded mining & energy companies.
Online writing for the Applied Arts Wire. My roles included interviewing and writing pieces on significant participants in the design community, including Cause + Affect (Vancouver), Dynamo (Montreal), Dashboard (Toronto), and Revolve (Halifax).
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Media coverage for TAXI during Icograda Vancouver Design Week 2010.
I graduated from this program in 2010 with a bachelor degree.


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Tuscan Hills Villa Rental Photos and Description
Villa, 5 Bedrooms, 3.5 Baths (Sleeps 10)
Courtyard Villa - Perfect Accommodation For 2 Families
Play, relax, and enjoy all the amazing attractions and activities in Central Florida. Then come home to swim in your own private southeast-facing pool or relax in the Jacuzzi and enjoy all the wonderful amenities this home has to offer. A beautifully decorated home with a tropical touch!
2 Luxurious King Master Bedrooms, each en-suite - new 2014
Living Room - New furniture and décor 2014
Digital 52' HD TV
Xfinity cable TV, WIFI HI Speed Internet, Phone and Home Security System
DVD Players
Private 26' X 13' pool with separate jacuzzi area(jointly heated to same temperature) Heat is optional and recommended November through March
Games Room with Ping Pong, Air Hockey, Darts, Basketball Toss
Use of Clubhouse Facilities - Gym, Pool Table, Foosball, Kiddie play yard, Tennis & Volleyball Courts
Free local and nationwide telephone calls
Private Laundry facilities
Free use of outdoor Gas Barbeque
- Suitability:
- Minimum Age Limit for Renters
- children welcome
- limited accessibility
- non smoking only
About Tuscan Hills Villa
We are a married couple from New Jersey who enjoy travelling. After 20+ years of staying at Disney hotels, we found renting a vacation home, and now owning a vacation home, the best way to experience the best of 'both Worlds'.
Tuscan Hills Villa purchased this villa in 2011
Why Tuscan Hills Villa chose Tuscan Hills
Location! Location! Location! Close to DisneyWorld, yet just a small commute to all Central Florida attractions. Tuscan Hills is a quiet gated community that is tastefully designed with a quiet ambiance, manicured landscaping and easy accessibility to many highways. Many conveniences and restaurants nearby. And to top it off, our home was given the Flipkey Gold 2013 Top Vacation Rental award. See more info/reviews at Flipkey.com
What makes this villa unique
Renting a home affords the traveller the opportunity to save money, expand the living experience, cook-in or BBQ, and enjoy a private swim during a mid-afternoon break or late at night. Plenty of activities within the home or clubhouse for non-park days. And Tuscan Hills... well its just the best, highly sought after for both rentals and home ownership!
Guestbook comments from the manager
5 stars for this home. Booking experience easy and owner very helpful and quick to respond to any questions. A lovely, clean and well equipped villa in an ideal location to explore all that the area has to offer. Very good local amenities close by. Villa perfect for our needs, just a little tricky to see the dial on the key safe without a light, when returning at night.