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Student News
Academic Success Tip - Stress Resilience (Cont'd)Posted November 18th, 2011 by Kimberly K. Ballard
It's that time in the semester when stress begins to escalate to new levels of intensity. However, now is also the time when you need to use your best stress resilience skills. Stress that is out of control can lead to illness, anxiety, lessened concentration, lack of sleep, and many other problems. Below are a few more tips on managing your stress for the remainder of the semester.
- Remember to look at the pieces and not the whole. Focus on one small task at a time. List all of the topics that you need to review for each exam course. List all of the research, writing, and editing tasks that you need to complete for a paper. Then focus on one small task at a time until that small task is complete. Cross it off the list and move on to the next small task. Step by step you can do it all.
- Ask for help if you are feeling overwhelmed. Talk to your professors about areas of the course that are confusing you. Talk to a counselor at the Student Counseling Center. Talk to a physician if you are having physical problems. Talk to your family. If your test anxiety is especially serious or long-standing, make an appointment with the Counseling Center to discuss additional techniques. 852-6585
- Sleep at least 8 hours a night. You will be more productive when you study. You will be able to focus on the essentials. You will be able to make wiser decisions about your priorities for studying. You will feel less helpless and hopeless. You will be less likely to burst into tears or yell at everyone around you. You will go into exams well-rested and alert.
- Add exercise to your schedule if you have let it go. Exercise is one of the best stress busters you can use. Try to get a minimum of three 30-minute workouts a week. Consider where a study break can include an exercise break. Even walking around the building or the campus can be a boon to your brain cells for memory and your body for sleeping better.
Academic Success Tip - Stress ResiliencePosted November 17th, 2011 by Kimberly K. Ballard
It's that time in the semester when stress begins to escalate to new levels of intensity. However, now is also the time when you need to use your best stress resilience skills. Stress that is out of control can lead to illness, anxiety, lessened concentration, lack of sleep, and many other problems. Below are a few tips on managing your stress for the remainder of the semester.
- Put grades in perspective. Grades are not equal to who you are as a person. You have knowledge of the course, talents, character strengths, and other skills that go beyond that one exam. We have many extraordinarily gifted and successful graduates in law practice and on the bench who will openly state that they graduated in the "great middle" of their class. For courses with the only grade attached to the final exam, the grade only measures how you performed on that limited set of questions on that day during 3 or 4 hours.
- All you can ask of yourself is to do the best that you can. No one can do better than his or her best. The important thing is to do as well as you can within your intellectual capabilities, talents, and skills. Our best does not always get us an "A" grade, but it does get us self-respect.
- Do not dwell on what you have no control over. You cannot control the days of your exams in most cases. You cannot control the questions on your exams. You cannot control paper deadlines. You cannot control how a professor grades. Do not waste time wishing that you had control over these things. Focus instead on what you can control.
- Do not dwell on what you did not do earlier in the semester. You cannot change that you did not study enough during the first part of the semester. You cannot change that you did not choose the best course load. You cannot change that you did not find a study partner earlier. You cannot change that you did not do practice questions earlier. You cannot change that you did not outline earlier. Regrets get you no further along the path to exam success. Make a note to change next semester. But for now, spend time and energy focusing on what you can still control.
- Take control of what YOU can control. You can ask your professors questions about material you do not understand. You can study with classmates to gain new or broader perspectives on the material. You can ask a professor to look at an outline to see if you are on the right track. You can do more practice questions. You can set up a structured time management schedule for each remaining week to distribute tasks more effectively. You can use study aids to help you understand material that is still vague. You can use breaks to increase your focus during intense study periods. You can use rewards to keep yourself motivated.
Brandeis "BRIEF" Break - TODAY!Posted November 16th, 2011 by Kimberly K. Ballard
New Public Service Opportunity AvailablePosted November 16th, 2011 by Jina A. Scinta
Network Center for Community Change is looking for students to help with a study to determine what happened to homes and homeowners facing foreclosure in 2007. If you help with this study, you will be helping to gather the information they need to describe the full impact of the foreclosure crisis on neighborhoods. Students will help by driving to affected properties to report on their condition, performing title searches, reviewing court files, doing research on the Property Value Administrator's website, and more. They will provide any training necessary before the students perform outreach work.
Interested students can go to the referenced study so you can evaluate if this is something you would like to help with. The study is available at: http://www.metropolitanhousing.org/wp-content/uploads/member_docs/2008_Louisville_Foreclosure_Crisis.pdf
This project would be good for first year students who weren't able to participate in the week-long projects. This would also be suitable for students still needing to complete their requirement before the graduation deadline.
To sign up, please contact Jina Scinta at firstname.lastname@example.com to obtain a Reservation Form.
MCB Congrats!Posted November 16th, 2011 by Anonymous
Cookies and Coffee - Brandeis "Brief" BreakPosted November 16th, 2011 by Kimberly K. Ballard
Academic Success Tip - Practice QuestionsPosted November 16th, 2011 by Kimberly K. Ballard
Paid Summer Internship Opportunity for Law and other Graduate StudentsPosted November 16th, 2011 by Jina A. Scinta
The Jefferson County Family Court Administrator has advised us that the Barton Center is now accepting applications for the 2012 Emory Summer Child Advocacy Program. Law students currently in their first or second year, and students in Masters of Social Work, Masters of Public Health, or other related graduate programs are encouraged to apply. The Emory Summer Child Advocacy Program (ESCAP) is a partnership between Emory University and the Georgia Department of Human Services Division of Family and Children Services (DFCS) to (1) provide students with a hands-on learning opportunity in the field of child advocacy, and (2) to provide child advocacy professionals with the benefits of student interns during the summer months. Students receive a summer stipend to work with judges, lawyers, DFCS professionals, or nonprofit organizations in Georgia for ten weeks.
Interested students can learn more by reviewing the attached for more details and application instructions.
MCB Good Luck!Posted November 15th, 2011 by Anonymous
MCB ResultsPosted November 15th, 2011 by Anonymous
Please join the Moot Court Board in congratulating Nathan Batey, Chris Moncrief, Sharon Wright, and Shawn Spalding (Team 1), and Jamie Jackson, Thomas Stevens, Brian Smith and Tyler Fleck (Team 2) on their strong performance at this past weekend's Kentucky Intrastate Mock Trial Competition in Lexington.
Under the guidance of coaches Sandra Moon and Heend Sheth, all eight members worked extraordinarily hard leading up to and during the competition. The many hours of preparation paid off, with Team 2 advancing all the way to the final round against the University of Kentucky College of Law's team. The Moot Court Board is proud to have had such stellar students representing our law school so exceptionally well.


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The minimum qualifications to apply to this program are:
- US citizen or permanent resident status
- Satisfactory completion of a US Residency in Neurology, Psychiatry, or fellowship in Infectious diseases
All applying trainees must submit the following documents by December 31 of the year prior to matriculation:
- Three letters of recommendation (one from Residency/ID Fellowship Director)
- A one- to two-page essay describing your interest in pursuing research training, the specific track you are interested in, and a statement of your scientific interests
Documents may be mailed to:
The Johns Hopkins University
School of Medicine
ATTN: Justin McArthur
600 North Wolfe Street
Meyer 6-109
Baltimore, MD 21287
Submissions will be solicited each August for November 15th deadline. Applications will be made with the help of a pre-identified prospective mentor, wherever possible. The applications should briefly define the specific research project planned, the mentoring laboratory or clinical resources to be utilized, and the specific educational goals sought by the applicant.
When all applications have been received, the Executive Committee will distribute the applications to a primary reviewer based on the training program track. Once all applications have been reviewed, and Executive Committee meeting will be held to discuss all of the applicants. The strongest applicants will be identified and final selections will be made for interviews.
Decisions will be made by January 1 for matriculation July 1.
Contact us
To learn more about Johns Hopkins Department of Neurology: Neuroinfectious Disease Fellowship, please call: 443-287-0571.

subset: commoncrawl


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November 27, 2014
A federal judge says lawsuits by two men falsely accused of murder can proceed against southeast Nebraska law enforcement officials.
Matthew Livers and Nicholas Sampson sued Douglas County and Cass County law enforcement officials in 2008 and 2007, saying officials violated their constitutional rights.
The two men were initially charged in the 2006 shotgun slayings of Wayne and Sharmon Stock in their farmhouse in southeastern Nebraska. The men spent months in prison before being cleared of murder charges.
A Wisconsin pair was eventually convicted and sentenced to life in prison.
U.S. District Judge Joseph F. Bataillon on Friday rejected a request by the defendants to dismiss the lawsuits. Trial in the case has been set to begin Oct. 21.
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FORT MYERS, Fla.- Fort Myers Police are still on the hunt for whoever broke into a woman's home and sexually assaulted her while a child was hiding in the home. They have increased the reward in this case to $5,000 for anyone who provides information that leads to the arrest and conviction of the person responsible.
The home invasion happened at the intersection of Edgewood Avenue and Van Buren Street.
FMPD released surveillance of the suspect's car, hoping someone will come forward with information about the man. The video came from a neighbor's security camera and shows what is believed to be the suspect's car. Police said it's a newer model white four-door Toyota Camry with no window tint.
In the video, you can see the vehicle pull up to the victim's house, according to police, around 10:30 a.m. yesterday. The man reportedly entered the victim's home and attacked her while a 3-year-old hid elsewhere in the home.
The suspect is described as a man of medium height and build with dreadlock-style hair and a green t-shirt.
A neighbor said that man actually ransacked his sister's home first, and now she's fearful that he's still on the loose.
Anyone with information is asked to call SWFL Crime Stoppers at 1-800-780-TIPS.


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Gold Winner - Outdoor Space. Photo by Michael Kella.
|Gold Winner:
Pathway to Heaven
Most of us would be happy with a backyard containing a pool, a patio, maybe a cabana and bar area, and a couple of speakers so we could listen to some tunes while we relaxed and entertained. Not the owners of this Southern California playground. Their backyard entertainment is so extensive, they need a golf cart path to link the 3.5 acres of fun.
|Silver Winner:
When Nature Calls
There's no reason to reserve a room at a fancy resort when you've got a backyard like this to enjoy. Music blankets the entire grounds, and colorful accent lights play up ornamental trees, fountains, water gardens and a spectacular infinity-edge swimming pool.
|Bronze Winner:
Home Sweet Casita
Can you say casita? If you live in a sunny climate, you better be able to say it, learn it, be it. Because you, too, can 'casita' all your home entertainment pleasures.


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November 27, 2014
Dozens of community gardens across the metro are now accepting new gardeners. The increase in the local foods movement led to a gardening boom in Omaha allowing renters and new urban farmers the opportunity to grow their own fruits and vegetables.
The Douglas County Health Department created and leads the local community garden network. Its website, douglascounty-ne.gov has a feature that includes a map of all community gardens and farmers markets. Visitors simply type in their address and a map will show them their neighborhood gardens and markets.
Click here if you have land and an interest in starting a community garden.
Among the many benefits, according to the Centers for Disease Control and Prevention, you can burn 150 calories by gardening (standing) for approximately 30-45 minutes.
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The United Methodist Church in Sierra Leone
by Alma Graham
The United Methodist Church, through its predecessor denominations, has had a mission presence in Sierra Leone for more than two centuries. Some Methodists went there as early as 1792, when a Briton, Thomas Clarkson, brought 1100 freed Blacks from Nova Scotia, Canada, to settle in the area. Thus "when George Warren, a Wesleyan Methodist missionary from Great Britain, arrived in Sierra Leone in 1811, [he] 'found the whole apparatus of Methodist organization and discipline already in progress,' with a 200-member strong Society." He also found a larger colony of freed slaves, including 500 free Blacks who came from Jamaica in 1800. Between 1807, when Britain abolished the slave trade, and 1865, when the US Civil War ended, 50,000 West Africans released by the British from slave ships had also settled in Sierra Leone.
The United Methodist Church in Sierra Leone today traces its history to 1855, when the Church of the United Brethren in Christ began mission work there. After a Black couple, Mr. and Mrs. Gomer from Dayton, Ohio, went to Shenge, Sierra Leone, in 1870, the mission began to flourish--particularly in education. As indigenous leaders were trained, many schools were developed, such as Albert Academy for boys in Freetown (founded 1904) and Harford School for Girls in Moyamba. Their graduates were to become national leaders when Sierra Leone became independent.
Soon the churches grew and they were pastored by African clergy. Rotifunk Hospital and many health dispensaries were strategically placed throughout the country. By the late twentieth century, the United Methodist Church in Sierra Leone was one of the largest Christian denominations in the country. The Sierra Leone Annual Conference was established in 1973 under the leadership of Bishop Thomas Bangura. It became a part of the West Africa Central Conference in 1981 and has been led by Bishop Joseph C. Humper since 1992. The conference has been actively engaged in education, agriculture, health, women's training centers, and clean- water systems. A vigorous program of evangelism has undergirded all these programs.
Operation Classroom, a program started in 1987 by United Methodist churches in several US states, provides schools, health centers, and clinics in Sierra Leone and Liberia. It is supported by the Indiana, Minnesota, North Carolina, Great Rivers, and Holston annual conferences and by designated contributions given through the Advance for Christ and His Church.
A military coup in May 1997 ousted the elected government and plunged the country into an ongoing civil conflict. The 12 Operation Classroom schools in Sierra Leone had to close, and now many schools have been destroyed in the fighting and students are attending classes in church buildings. In the spring of 1999, the capital, Freetown, was in turmoil. The rebels were targeting church leaders and members, and Bishop Humper had to go into hiding. The rebels killed thousands of civilians and mutilated hundreds more, often by cutting off their hands. Today, a United Nations peacekeeping force is trying to implement a 1999 peace accord, but rebel soldiers continue to attack civilians and few have turned in their weapons.
Donations to help Sierra Leone may be made to UMCOR Advance #181205- 1, Sierra Leone Emergency, and sent to UMCOR, General Board of Global Ministries, 475 Riverside Drive, Room 330, New York, NY 10115.
This article is drawn from New World Outlook, May-June 1994, Jan.-Feb. 1998, Mar.-Apr. 1999, and other sources. Copyright New World Outlook. For reprint permission, write to email@example.com


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November 21, 2014
ATLANTA (AP) - Andre Iguodala hit a 3-pointer at the buzzer, David Lee scored 23 points and the Golden State Warriors won their eighth straight game with a 101-100 victory over the Atlanta Hawks on Friday night.
Stephen Curry added 22 points with nine assists and Klay Thompson 21 for Golden State, which recovered from a 15-point deficit in the fourth quarter to get back in the game and extend its road winning streak to five straight.
Pero Antic finished with a career-high 16 points and Shelvin Mack had 15 for the Hawks. Atlanta had won three of four and six of eight.
Iguodala was inbounding the ball with 2.8 seconds remaining near the Hawks' bench and had 6-foot-11 center Antic defending him when he passed the ball to Curry near the halfcourt line.
Copyright 2014 Associated Press. All rights reserved. This material may not be published, broadcast, rewritten, or redistributed.
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D. A. Parker, farmer; P. O. Westfield, Clark Co.; is one of the pioneers of this county, and was born in Butler Co., Ohio, April 27, 1815; in the year 1817, his parents moved to Crawford Co., Ill., ten miles south of Palestine, and, after remaining there eight years, moved to Hutton Tp., Coles Co., and settled on what was afterward known as " Parker Prairie," in the year 1825, where his parents died, , his mother on the 11th day of December, 1830, and his father on the 18th day of March, 1862; they were among the first settlers, and entered the first land in this county. Mr. Parker remained with his parents until he was 21 years of age, when he went to farming for himself, and, on the 29th day of March, 1837, married Miss Mary J. Flint, daughter of Thomas Flint, of Kentucky; she was born in Virginia March 30, 1819. Mr. Parker settled on his present farm in the year 1840, having entered the land, and has resided there ever since, now containing sixty-four acres. His family consisted of nine children, five living – two boys, George W., born Feb. 8, 1839, and Allen D.. born May 17, 1852, and one deceased-Leroy, born April 19, 1850, died in 1854; six girls, three living – Sallie M. (now Mrs. J. B. Redmon, of Hutton Tp.), born Oct. 15, 1844; Ellen, Sept. 5, 1854, and Emma, born March 20, 1860, and three deceased– Elizabeth, born July 8, 1841, died Aug. 11, 1852; Martha J., born May 27, 1846, died in the fall of 1855, and Frances A., born Sept. 20, 1856, and died May 20, 1860.
Biographical Sketch of D. A. Parker
MLA Source Citation:Chapman Brothers Portrait and biographical album of Coles County, Illinois Chicago: Chapman Brothers, 1887. AccessGenealogy.com. Web. 20 November 2014. http://www.accessgenealogy.com/illinois/biographical-sketch-of-d-a-parker.htm - Last updated on Oct 20th, 2011
Categories: Illinois, Ohio
Topics: Biography
Locations: Butler County OH, Coles County IL, Crawford County IL, Hutton Illinois
Surnames: Parker
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- freely available
- re-usable
Nutrients 2012, 4(8), 1137-1150; doi:10.3390/nu4081137
Published: 22 August 2012
Abstract: The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet ad libitum throughout the entire experiment and, between 100 and 120 days of age drank ad libitum tap water alone (normal diet; CT-ND and P4-ND) or containing fructose (10% w/v; CT-FRD and P4-FRD). At age 120 days, animals were subjected to a glucose tolerance test or decapitated. Plasma concentrations of various biomarkers and PMAT gene abundance were monitored. P4-ND (vs. CT-ND) rats showed elevated circulating levels of lipids. CT-FRD rats displayed high (vs. CT-ND) plasma concentrations of lipids, leptin, adiponectin and plasminogen activator inhibitor-1 (PAI-1). Lipidemia and adiponectinemia were high (vs. P4-ND) in P4-FRD rats. Although P4 failed to prevent FRD-induced hyperleptinemia, it was fully protective on FRD-enhanced plasma PAI-1 levels. PMAT leptin and adiponectin mRNAs were high in CT-FRD and P4-FRD rats. While FRD enhanced PMAT PAI-1 mRNA abundance in CT rats, this effect was absent in P4 rats. Our study supports that a preceding P4-enriched milieu prevented the enhanced prothrombotic risk induced by FRD-elicited high PAI-1 production.
1. Introduction
Fructose corn syrup has become a popular sweetener used in soft drinks and many other products of modern, massive daily consumption. In countries like the USA, estimated annual per capita fructose consumption rose from 64 g/day during the 1970s to 81 g/day at the end of the 1990s [1], with an additional increase in fructose intake (2.5 g/day) resulting from increased fruit and vegetable consumption. It has been postulated that such increased consumption could contribute to the current epidemics of human obesity, Type 2 diabetes mellitus and metabolic syndrome (MS) [2]. Several studies demonstrated that administration of a fructose-rich diet (FRD) to normal rats of either sex induces several features of the human phenotype of MS [3,4,5]. Regarding the mechanisms whereby FRD-intake induced MS, it has been suggested that the high intake of fructose increases the production of reactive oxygen species (ROS), thus enhancing general and adipose tissue (AT) oxidative stress (OS) [6]. As a consequence, the enlarged abdominal adipocytes of FRD fed rats overproduce leptin [7,8].
It has been reported that sex steroids modulate OS and particularly progesterone (P4) has been postulated as a positive instigator of OS [9], probably by an indirect mechanism secondary to the activation of glucocorticoid receptors [10]. It is openly accepted that AT-derived adipokines regulate gonadal function, and that sex steroids such as testosterone [5,11] modulate white adipocyte endocrine function; however, little is known about potential P4 activity on AT function. Although lipogenic P4 activity has been reported in isolated adipocytes [12,13], and in vivo in the normal female rat [14], P4 has also been identified as a stimulator of insulin degradation at the pancreatic level [15]. Contrary to the effect of androgens, P4 did not prevent pancreatic cell death [16]. P4 could also act as an inhibitor of glucose-stimulated insulin secretion from pancreatic islets [17]. P4 treatment, either alone or in combination with estradiol (E2), modulates insulin sensitivity [18,19] without affecting glucose transporter (GLUT)-4 activity [18]. Moreover, other studies indicate that P4 impairs insulin sensitivity by antagonizing insulin action [20] and that this steroid is able to counteract the hyperglycemic and lipolytic effects of glucagon in the post-prandial state [21].
The aim of the present study was to explore whether a previous and transient P4-rich endogenous environment in the early post-pubertal female rat could modulate subsequent development of several metabolic, endocrine and adipose tissue dysfunctions induced by the intake of a FRD.
2. Experimental Section
2.1. Animals and Experimental Design
The estrous cycle of individually housed, adult Sprague-Dawley rats was daily checked for a week prior experimentation, thus rats do not displaying a complete estrous cycle were excluded from experimentation. Animals were kept in a temperature (21 °C)—and light (lights on 07:00 to 19:00 h)—controlled room with free access to Purina Rat Chow (normal diet; ND) and water. The sex steroid-priming protocol used in this study was previously developed in our laboratory for testing the effect of early intervention with a sex steroid (testosterone) on later metabolic-endocrine functions in the early post-pubertal female rat [11]. Briefly, randomly cycling rats were injected intramuscularly (i.m.) with 100 μL of sterile corn oil alone (control, CT; n = 20 animals) or containing Progesterone (12 mg/kg, P4; n = 24 rats) on day 60 of age. Rats were left undisturbed for 40 days (between age days 60 and 100). Thereafter, all rats were switched to drinking a fructose 10% (w/v) solution in water (FRD) while eating ad libitum Purina rat chow for 21 days (between age days 100 and 120) [5]. Fresh fructose solution was provided daily and, energy intake and body weight were recorded daily throughout the study. Animals were either decapitated between 8:00 and 10:00 h on day 120 of age in non-fasting conditions or after overnight fasting subjected to an i.v. glucose-tolerance test. Parametrial adipose tissue (PMAT) pads were dissected from rats sacrificed in non-fasting condition, weighed and kept frozen (at −80 °C) until processed. Additionally, for the purpose of avoiding any confusing effect of P4 treatment by itself, oil (CT)- and P4-treated rats were provided drinking water and normal chow diet (ND) ad libitum throughout both experimental periods (between age 60 and 120 days; these groups were abbreviated as CT-ND and P4-ND; n = 12–14 rats per group) (see Table 1). Experiments complied with international regulations concerning the ethical use of animals, and were also approved by our Institutional Animal Care Committee.
|Table 1. Summary of the experimental design.
|Treatment (i.m. on age 60 days)
|Diet (100–120 days of age)
|Group (abbreviation)
|Fructose Rich
|Fructose Rich
|Experimentation (on day 120 of age)
2.2. Peripheral Metabolite Measurements
Plasma glucose (Wiener Argentina Lab.), triacylglycerol (TG; Wiener), total cholesterol (Wiener) and non-esterified fatty acid (NEFA; Randox Laboratories Ltd., UK) levels were measured using commercial kits. Plasma leptin [22], insulin [23], corticosterone [24], E2 [24] and testosterone (T) [24] concentrations were determined by specific radioimmunoassays (RIAs) developed in our laboratories, while those of P4 were measured using a commercial RIA kit (Immunotech Laboratories, Inc. Glendale, CA, USA). Coefficients of variation (CVs) intra- and inter-assay for all RIAs, ranged between 4% and 6% and 8% and 12%, respectively. Plasma levels of other adipokines were assayed by following the recommendations of the respective retailers of different ELISA kits (Linco Research, Cat. #EZRADP-62K for adiponectin, ADIPOQ; and American Diagnostica Inc., CT, USA, IMUCLONE Cat. #601 for plasminogen activator inhibitor factor-1, PAI-1).
2.3. Intravenous Glucose Tolerance Test (i.v.-GTT)
Metabolic responses to a high i.v. glucose load (2 g/kg BW) were measured in rats under light ketamine anesthesia. Rats were bled before (sample time 0) and several times (5, 15, 30, 60 and 90 min) post-glucose administration [25]. Plasma samples were kept frozen (−20 °C) until determination of glucose and insulin concentrations as described above.
2.4. Parametrial Adipose Tissue RNA Isolation and Real-Time Quantitative PCR
Total RNA was isolated from PMAT pads of rats from different groups by the single-step, acid guanidinium isothiocyanate-phenol-chloroform extraction method (Trizol; Invitrogen, Life Tech., USA; cat. #15596-026) [26]. One µg of total RNA was reverse-transcripted using random primers (250 ng) and Superscript III Rnase H-Reverse Transcriptase (200 U/μL Invitrogen, Life Tech, USA; cat #18989-093). Primers applied: β-actin (ACTB) (R): 5′-ACCCTCATAGATGGGCACAG-3′, (F): 5′-AGCCATGTACGTAGCCATCC-3′ (115 pb) (GenBank Accession Number (GBAN): NM_031144); LEP (R): 5′-CTCAGCATTCAGGGCTAAGG-3′, (F): 5′-GAGACCTCCTCCATCTGCTG-3′ (192 pb) (GBAN: NM_013076); ADIPOQ (R): 5′-TCTCCAGGAGTGCCATCTCT-3′, (F): 5′-AATCCTGCCCAGTCATGAAG-3′ (159 pb) (GBAN: NM_144744); PAI-1 (R): 5′-TCTCCAGGGGCCCTCTGAGGT-3′, (F): 5′-TGCCCCTCTCCGCCATCACC-3′ (141 pb) (GBAN: NW_047370); IRS-1 (R): 5′-ACGGTTTCAGAGCAGAGGAA-3′, (F): 5′-TGTGCCAAGCAACAAGAAAG-3′ (176 pb) (GBAN: NM_012969); and GLUT-4 (R): 5′-TGGACGCTCTCTTTCCAACT-3′, (F): 5′-GCTTCTGT-TGCCCTTCTGTC-3′ (166 pb) (GBAN: NM_012751). Two microliters of the reverse transcription mix were amplified with QuantiTect Syber Green PCR kit (Qiagen, cat. #204143) containing 0.5 μM of each specific primer using a LightCycler Detection System (MJ Mini Opticon, Biorad). PCR efficiency was ~1. Cycle thresholds (Ct) were measured in separate tubes by duplicate. Identity and purity of the amplified product were checked by electrophoresis on agarose mini-gels and the melting curve was analyzed at the end of amplification. Values of the differences between the cycle threshold (Ct) were calculated in every sample for each gene of interest as followed: Ct (gene of interest) − Ct (reporter gene). β-actin, whose mRNA levels did not differ comparing control to test groups, was the reporter gene. Relative changes in expression level of one specific gene (ΔΔCt) were calculated as ΔCt of the test group minus ΔCt of the control group and then presented as 2−ΔΔCt.
2.5. Statistical Analysis
Data (means ± SEM) were analyzed by ANOVA (one-factor (treatment) or two-factor (treatment × diet) when appropriate) followed by Fisher's test. The nonparametric Mann–Whitney test was used to analyze data from PMAT mRNA expression [27]. p values lower than 0.05 were considered statistically significant.
3. Results
3.1. Effect of Diet on Body Weight, Parametrial Adipose Tissue Mass and Energy Intake in CT and P4-Primed Rats
Figure 1A shows that P4 treatment did not modify individual body weight (BW) in rats normally nourished (ND) (between age days 60 and 100). Moreover, P4 treatment did not modify rat BW when fed a FRD (between age days 100 and 120). On the experimental day, BW (224.61 ± 4.19 and 219.33 ± 5.18 g) and PMAT mass (1.64 ± 0.09 and 1.79 ± 0.11 g/100 g BW) were similar (p > 0.05) in CT-FRD and P4-FRD rats (n = 6/7 rats per group).
The average rat daily energy intake was not influenced by P4 treatment (between age days 60 and 100; in kJ/day/100 g BW; 105.95 ± 5.17 in CT and 104.47 ± 6.05 in P4 rats). Thereafter, the 21-day average daily energy intake during the FRD administration period (between days 100 and 120 of age) to rats was also similar in the two groups (in kJ/day/100 g BW; 114.68 ± 3.44 in CT-FRD and 107.71 ± 4.59 in P4-FRD rats); being energy intake statistically similar (p > 0.05) in both periods regardless of treatment and diet; as well as after compared the 60 day average-period (in kJ/day/100 g BW, 108.11 ± 1.73 in CT and 106.53 ± 1.88 in P4 rats; p > 0.05). Interestingly, P4-primed rats displayed some hedonic behavior for the FRD that only lasted for few days (Figure 1B). Thereafter, P4-FRD animals recovered a normal daily energy intake, and even showed a lower daily energy intake (vs. CT-FRD rats) on a few days of the FRD intake period, thus compensating for a normal 21-day average energy intake (Figure 1B). Nevertheless the 21-day average daily fluid intake was similar in both groups (in mL/day/rat; 36.8 ± 1.2 in CT-FRD and 35.1 ± 2.1 in P4-FRD rats; p > 0.05).
3.2. Effect of FRD Intake on Peripheral Biomarkers in Normal and P4-Primed Rats
We found that non-fasting peripheral levels of glucose, insulin, progesterone, total cholesterol, corticosterone, estradiol and testosterone were similar in CT and P4 rats regardless of the diet administered (Table 2). Conversely, P4 treatment (P4-ND rats) already induced a significant (p < 0.05 vs. CT-ND values) increase in plasma concentrations of TG and NEFA (Table 2). Although plasma total cholesterol concentrations were not influenced by FRD intake in CT rats, it was significantly (p < 0.05) higher in P4-FRD than in P4-ND and CT-FRD rats (Table 2). Interestingly, P4-primed rats already displayed higher (p < 0.05 vs. CT-ND values) plasma triacylglycerol levels when fed a ND (Table 2) and circulating levels of TG significantly (p < 0.05 vs. their respective ND group-values) increased after fructose administration in both groups (CT-FRD and P4-FRD) (Table 2), a parameter aggravated by P4 in FRD fed rats (p < 0.05 for P4-FRD vs. CT-FRD values, see Table 2). Similarly, priming rats with P4 (P4-ND rats) induced a significant (p < 0.05 vs. CT-ND values) rise in peripheral NEFA concentrations when rats were fed a ND, and intake of a FRD significantly (p < 0.05 vs. CT-ND values) increased peripheral NEFA concentrations in CT rats (Table 2).
|Table 2. Circulating levels of several metabolites in control (CT) and progesterone-primed (P4) rats examined 21 days after either ND or FRD intake. Values are means ± SEM, n = 6–7 rats per group.
|Glucose (mM)
|6.33 ± 0.28
|6.01 ± 0.33
|6.72 ± 0.31
|7.38 ± 0.36
|Insulin (nM)
|6.17 ± 1.53
|6.94 ± 0.68
|5.51 ± 0.61
|5.39 ± 0.72
|Total Cholesterol (mM)
|1.64 ± 0.13
|1.58 ± 0.08
|1.34 ± 0.15
|1.91 ± 0.12 b,c
|Triacylglycerol (mM)
|0.99 ± 0.08
|1.41 ± 0.09 a
|1.44 ± 0.22 a
|1.97 ± 0.18 c
|NEFA (mM)
|0.35 ± 0.04
|0.53 ± 0.06 a
|0.51 ± 0.05 a
|0.52 ± 0.07
|Corticosterone (μM)
|0.35 ± 0.06
|0.45 ± 0.08
|0.33 ± 0.09
|0.43 ± 0.09
|Progesterone (nM)
|37.34 ± 8.14
|48.31 ± 14.22
|47.16 ± 9.51
|31.36 ± 7.89
|Estradiol (nM)
|0.19 ± 0.03
|0.20 ± 0.04
|0.22 ± 0.02
|0.26 ± 0.03
|Testosterone (nM)
|0.86 ± 0.06
|0.98 ± 0.05
|0.95 ± 0.08
|0.91 ± 0.17
a p < 0.05 vs. CT-ND values; bp < 0.05 vs. P4-ND values; cp < 0.05 vs. CT-FRD values.
Regarding plasma adipokine levels, we found that leptin values were similar in CT-ND and P4-ND, and that they were significantly (p < 0.05) enhanced after FRD intake in both groups. However, the leptinemia attained by P4-FRD rats was even higher (p < 0.05) than that reached by CT-FRD rats (Figure 2A). While circulating adiponectin levels were similar in both ND groups, they were significantly (p < 0.05 vs. respective ND group-values) higher after FRD intake regardless of the group examined (Figure 2B). Conversely, although similar in both groups of rats fed a ND, plasma PAI-1 levels were significantly (p < 0.05 vs. CT-ND values) enhanced by FRD intake in CT but not in P4 rats (Figure 2C).
3.3. Changes in Plasma Glucose and Insulin Concentrations throughout the i.v.-GTT
Data from the i.v.-GTT indicate that although overnight fasting did not modify basal (time zero values) plasma glucose concentrations (Figure 3A), it significantly (p < 0.05 vs. CT-FRD values) reduced basal plasma insulin levels in P4-FRD rats (Figure 3C). After a high (2 g/kg BW) glucose load and despite similar plasma 5 min peak values of glucose in both groups examined, circulating glucose and insulin concentrations were significantly (p < 0.05) higher and lower in P4-FRD than in CT-FRD rats, respectively. Moreover, P4-FRD animals had not recovered basal plasma values of glucose by the end of the test (Figure 3A) and starting plasma insulin concentrations were reached 30 min later in P4-FRD than in CT-FRD rats (Figure 3C).
As expected, the areas under the curves (AUC) of plasma glucose (Figure 3B) and insulin (Figure 3D) concentrations throughout the test were significantly (p < 0.05) higher and lower in P4-FRD than in CT-FRD rats, respectively.
In order to avoid any confusing effect of the earlier P4 treatment, additional studies were run on CT and P4 rats fed a ND (CT-ND and P4-ND rats). These data indicated that P4 treatment did not modify any parameter during the i.v.-GTT (n = 5–6 rats per group). In fact, overnight fasting-induced insulinemia was similar in both groups (2.39 ± 0.68 and 2.11 ± 0.59 nM; in CT-ND and P4 ND, respectively). Moreover, no group-differences were found in the AUC values of both glucose (973.03 ± 52.23 and 997.39 ± 98.57 mmol/L/90 min; in CT-ND and P4 ND, respectively) and insulin (2020.98 ± 406.61 and 1793.95 ± 411.24 nM/90 min; in CT-ND and P4-ND, respectively).
3.4. Effects of P4 Treatment and FRD on PMAT Genes
PMAT leptin, adiponectin and PAI-1 mRNA expression levels (Figure 4A,B,C) were significantly (p < 0.05 vs. CT-ND group values) higher in CT-FRD rats. FRD administration in P4-treated rats also enhanced (p < 0.05 vs. P4-ND group-values) PMAT leptin and adiponectin mRNA abundance (Figure 4A,B). Moreover, these increases were even higher (p < 0.05) than the respective values found in CT-FRD group (Figure 4A,B). Conversely, administration of a FRD to P4-primed rats did not enhance PMAT PAI-1 mRNA expression, an effect fully independent of any previous change induced by P4 treatment (see CT-ND and P4-ND group-values in Figure 4C).
Finally, feeding rats a FRD did not induce any significant group-difference in the abundance of both GLUT-4 (in arbitrary units, AU; 1.09 ± 0.16 and 1.32 ± 0.32 in CT-FRD and P4-FRD groups respectively; n = 4 rats per group, p > 0.05) and IRS-1 (in AU; 1.07 ± 0.09 and 0.84 ± 0.13 in CT-FRD and P4-FRD groups respectively; n = 4 rats per group, p > 0.05) mRNA levels in PMAT pads.
4. Discussion
In the present study we have shown for the first time that the metabolic, endocrine and adipose tissue dysfunction induced by short-term administration of a FRD to adult female rat can be prevented, at least partly, by previous P4 treatment. This P4 pretreatment unevenly affected the FRD-induced PMAT adipokine overproduction: whereas it enhanced leptin production and prevented PAI-1 production. We underscore that, unrelated to any intrinsic steroid effect, FRD rats treated with P4 had impaired glucose tolerance due to decreased high glucose-stimulated insulin secretion. Although the adipo-insular axis function in P4-FRD rats is well adapted in basal condition (i.e., normal plasma levels of glucose and insulin in non-fasting condition), with application of an allostatic load (e.g., high glucose load) the deleterious effect of P4 [15] on insulin release was observed.
Our current results support previously reported data related to metabolic, endocrine and AT dysfunction in normal adult female rats fed a FRD [5]. We now found that P4 administration to rats fed a standard diet (P4-ND) failed to modify plasma total cholesterol levels but enhanced those of TG and NEFA. Also, the FRD induced an increase in plasma cholesterol levels in P4-primed rats. These observations agree with previous data showing in vivo lipogenic activity of P4 in normal female rats [14], an effect ascribed to the ability of P4 to up-regulate fatty acid synthase activity [28]. Therefore, previous P4 administration could play a relevant role in the lipidic and endocrine dysfunctions induced by intake of a FRD.
The changes recorded in PMAT endocrine function in rats fed a FRD strongly depend on the host to which this allostatic load was applied: whereas in normal (CT) rats the FRD-induced increase in leptin plasma levels was aggravated by previous P4 administration, increased circulating levels of PAI-1 was prevented by P4 treatment. Conversely, the FRD-elicited change in adiponectin production was similar in the two groups.
It is accepted that the provision of an excess of a substrate (e.g., fructose) over a period of time increases NEFA production [29], AT OS [6,29] and several other AT dysfunctions [5,6,7]. Brownlee has postulated that substrate overload increases adipocyte-ROS mitochondrial production which results in multiple impairments, namely: (a) decreased antioxidant defense, (b) enhanced activity of protein kinase C isoform and PAI-1 production and (c) increased non-enzymatic glycosylation rate [30]. It has also been reported that the administration of a FRD induces OS that significantly affects AT [5,6,7] and other tissues functions [6,31]. Indeed, the FRD-elicited OS at the liver level triggers a local increase in glycogen storage, glucose-6-phosphate dehydrogenase activity, lactate production, glucokinase and glucose-6-phosphatase activities, thus leading to an increase in liver and plasma triacylglycerol levels [31]. All these changes might represent an adaptive defense mechanism against fructose overload in order to decrease substrate provision to the mitochondria, thus lowering ROS production. Also, the increased activity of the pentose pathway would provide the required NADPH for fatty acid synthesis, which then favors lipogenesis. Enhanced production of NADPH could also activate NADPH oxidase, thus establishing a vicious circle aggravating the overall OS induced by FRD intake.
We previously reported that administration of a FRD to normal rats induced an increase in AT mass at the expense of the size of its adipocytes [5,7] associated with leptin overproduction [5]. The latter dysfunction was currently enhanced in animals pretreated with P4. Since high leptin levels impair insulin secretion and its activity [32], an overproduction of adiponectin [5,33] would be a crucial compensatory mechanism to overcome these alterations consecutive to the hyperleptinemia observed in P4-FRD rats. Although P4 produced in FRD rats all these detrimental effects on AT lipid metabolism and leptin production [34,35], it exerted a significant protective effect on the high PAI-1 production induced by this diet. In this regard, although little information exists, synthetic progestins have been shown to be effective in reducing PAI-1 production, both in vitro [36] and in vivo [37,38], thus reducing the high risk for developing cardiovascular disease in post-menopausal women.
In summary, although the lipogenic effect of an excess of fructose intake throughout the diet remained/enhanced after previous P4 administration, our study supports that P4 effectively prevents the high PAI-1 production (plasma levels and AT mRNA concentration) induced by FRD administration to normal adult female rats. Our main finding is consistent with the previously described inverse relationship between endogenous PAI-1 production and peripheral progesterone concentration [39,40]. Because menopause heralds a drastic reduction in circulating levels of sex steroid hormones, this decline may contribute to increase cardiovascular risk by affecting adiposity, lipid metabolism and the prothrombotic state [41].
5. Conclusion
The present study supports the concept that P4 exerts a dual effect upon fat tissue metabolism: It enhances in vivo lipogenic activity, induced by administration of a FRD, and prevents the PAI-1 overproduction induced by this diet. However, we have yet to determine the precise mechanism whereby P4 exerts its modulatory effect upon nutrient-gene interaction and the potential partial adjustment of the nutrient-induced cell dys-metabolome [42].
This work was partly supported by grants from the National Research Council (CONICET; PIP 2009-0704) and National Fond for Science and Technology (FONCyT; PICT 2007-1051) of Argentina, and Fondation Pour la Recherche en Endocrinologie, Diabetologie et Metabolisme (FPREDM 2011–2012; to ES) of Switzerland. Authors wish to thank J.J. Gagliardino for his criticisms, and S.H. Rogers for correcting the manuscript.
Conflict of Interest
The authors declare no conflict of interest.
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© 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).


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Varicella-zoster virus (VZV) latency in human dorsal root ganglia is characterized by the transcription of large regions of its genome and by the expression of large amounts of some polypeptides, which are also expressed during lytic cycles. The immediate early 63 protein (IE63) is a virion component expressed very early in cutaneous lesions and the first viral protein detected during latency. Immune response against IE63 has been evaluated among naturally immune adults with a history of chickenpox: Specific antibodies were detected in serum, and most subjects who had a T cell proliferation with unfractionated VZV antigens had T cell recognition of purified IE63. The cytotoxic T cell (CTL) response to IE63 was equivalent to CTL recognition of IE62, the major tegument component of VZV, whose immunogenicity has been previously described. T cell recognition of IE63 and other VZV proteins is one of the likely mechanisms involved in controlling VZV reactivation from latency.
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International Journal of Speech Technology
The International Journal of Speech Technology focuses on speech technology and its applications. It promotes research into all aspects of speech input and output, including theory, experiment, testing, base technology, and applications.
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- International Journal of Speech Technology
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Welcome to the eighth installment of the Gimme Noise Beattape, a collection of sounds from Twin Cities producers and beatmakers. As the final weeks of summer slide past, a suitable soundtrack emerges. Here are some great beats from a new cadre of musicians, all of whom stem from the vibrant local sc ... More >>
This summer's Vans Warped Tour will boast some impressive and eclectic artists to draw fans of punk, hip-hop and hardcore right alongside the inevitable Hot Topic zombies. The venerable festival, hasn't always evolved smoothly, but this year's Minnesota lineup is particularly impressive. It comes to ... More >>
The writer Martin Amis once referred to a critic as "humorless," by which he meant to impugn on the man's seriousness. The censors employed by the United Arab Emirates government may not be serious people, but they have serious means of punishment at their disposal. Looking into the case of Shezanne ... More >>
This week not only marks Thanksgiving but also the beginning of December. Wednesday, Retribution Gospel Choir play the Amsterdam while the Ike Reilly Assassination take over the First Avenue Mainroom. If you're looking for plans on Thanksgiving, Davina and the Vagabonds will be playing the Dakota an ... More >>
It's finally starting to feel like May again, and this week is packed with shows. Friday Daughter visits the Fine Line with label-mate and local opener, Jeremy Messersmith. The Amsterdam hosts their second annual Girls Got Rhythm Fest featuring Cherie Currie, The Avengers and many more stretching ov ... More >>
Another Mission: Impossible movie
Don't put our fare in the flyover category
Minneapolis has a sweet tooth of late
What? Me worry?"Prairie Home Companion" host and erstwhile St. Paul bookstore owner Garrison Keillor was nowhere near Lake Wobegon Saturday night. No sir, he was taking in a Broadway show near Times Square in New York City when the guy he cheerfully refers to as the "Incompetent Bomber" fai ... More >>
The lauded bad boy of the culinary world talked smack about his competitors, offered his tips on travel, and, of course, complimented the Twin Cities' food scene.
Minnesota's crazy congresswoman was 2009's battiest politico
That's likely going to make for slow airline travel for Somalis in the Twin Cities.
Singapore Airlines and the Dubai-based Emirates rank tops for airline food according to a new Zagat poll.
Ridley Scott's latest delivers the post-9/11 tech-savvy terror thriller we deserve
The Bush Administration's plans for Iran, robots exploring the Antarctic, order your company's own anthem, Titanic 2: The Surface, the Minnesota Blog of the Day, and more...
Video games help brain dead kids, whiny kids become conservative adults, TPS Report coversheets, Childress disses Culpepper, Diablo on Dave, the Minnesota Blog of the Day, and more...
Everyone gets to go to UC Berkeley, Ice Age blondes had more fun, Dubai firm in port deal boycotts Israel, musicians say no to Hummers, the Minnesota Blog of the Day, and more...
Guess which former Minnesota congressman is de-scandalizing the scandal?
Hollywood auteur-cum-harlot cops a convoluted plea in 'Spartan'
The Venice Film Festival gives voice to the global cacophony


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Egmond aan Zee Apartment Rental Photos and Description
1 Livingroom, 1 Kitchens, 2 Sleepingrooms, 1 Bathroom
Only 3 minutes from the Northseabeach and Dunes, just in the mainstreet of the cosy villagecenter you'll find our beautiful vacation house wich has 2 Appartments with a livingroom/kitchen, 2 bedrooms and a bathroom.They can be rent separated for 2-4 persons but will fit also perfectly for greater or 2 familys together until 8 persons.. On the first floor there is a luxury new renovated appartment with a living-room that has a view on the cosy outside square and supermarket, kitchen, 2 bedrooms and an bathroom with toilet and shower.. And on the second floor there is a luxury new renovated appartment with a livingroom/kitchen, 2 bedrooma and bathroom with bath and toilet. Unter the appartments there is a locked box specially for keeping bicycles.
- Suitability:
- children welcome
- pets considered
- suitable for elderly or infirm


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August 25, 2014 Contact: Brandon Baier, 734-647-6109, firstname.lastname@example.com
Ann Arbor, MI – Lonnie D. Shea has been named professor and chair of the University of Michigan Department of Biomedical Engineering (BME), effective September 1, 2014.
Shea, who earned his PhD from U-M in 1997, was recruited from Northwestern University's Department of Chemical and Biological Engineering, where he has served on the faculty since 1999. He is an internationally recognized researcher at the interface of regenerative medicine, drug and gene delivery, and immune tolerance, whose focus is controlling the local microenvironment for directing tissue growth or regeneration. His projects include ovarian follicle maturation for treating infertility, islet transplantation for diabetes therapies, nerve regeneration for treating paralysis, autoimmune diseases and allogeneic cell transplantation, and cancer diagnostics. He is also developing and applying systems biology approaches to molecularly dissect tissue formation and identify key drivers of normal and abnormal growth.
Shea has received funding from the National Institutes of Health (NIH), National Science Foundation (NSF) and multiple foundations; has published more than 150 manuscripts on his research; and has numerous inventions to his credit, among them a cellular assay with which he can measure the activity of numerous transcription factors within the cell that reveal key signaling pathways as cells differentiate and develop in his customized 3D cultures.
In addition to his research and teaching responsibilities at Northwestern, Shea served as director of its NIH Biotechnology Training Grant and was a member of its Institute for BioNanotechnology in Medicine. He is a fellow of the American Institute of Medical and Biological Engineering (AIMBE), a standing member of the Biomaterials and Bionterfaces study section at NIH, and a member of the editorial boards for Molecular Therapy, Biotechnology and Bioengineering, and Drug Delivery and Translational Research.
Derek Tat, who was a Ph.D. student in Prof. Cindy Chestek's lab, passed away late last week in a vehicle accident. Derek was a brilliant student and close friend to the Chestek lab as well as the greater Michigan community. The entire U-M BME family is deeply saddened by the news of his tragic death.
Services for Derek will be held on Thursday, October 23, 2014 from 10:00 am – 12:00 pm at the Muehlig Funeral Chapel in Ann Arbor, Michigan. Derek's family welcomes all to attend his funeral services. The Chapel's address is:
Muehlig Funeral Chapel
403 S 4th Ave, Ann Arbor, MI 48104
If you would like to donate or share a memory about Derek, please visit this page:
All proceeds donated to the fund will be sent to the Tat family on November 1st.
Individual parts of the brain can be activated and de-activated by shining light on the neurons, and researchers are using this ability to chart how different areas of the brain function. To zoom in on individual neuron circuits within the brain, more precise light sources are needed. Euisik Yoon, a professor of electrical engineering and computer science at U-M, is leading a team that will design and build these new light sources with a variety of lasers.
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Hiv Positive Gay Dating
Here's a taste of what TheBody.com has to offer on this topic:
Connect With Others
HIV Positive Gay Dating for HIV Gay Men and Bisexual Men
Posted by Hneedlovehttp://www.PozGays.com is The first, completely anonymous and most trusted online network dedicated to gay men and bisexual men with HIV positive meeting new friends, partners or potential spouses in the world.
Over 20% gay and bisexual men are... Read more »
HIV Positive Gay Dating / Dating Gay Men with Herpes
Posted by pozladyhttp://www.PozKiss.com is an anonymous, warm-hearted and exclusive community offers dating and friendship options for people with a wide variety of personal preferences including interracial, gay, lesbian and bisexual singles and others with HIV and... Read more »
Poz Gay, Hiv Positive Gay Men and Bisexual Find gay with hiv, HIV Gay Dating, HIV Gay
Posted by Abivichttp://www.PozBuddies.com focuses on providing a safe Poz Match & Support group for HIV Personals. It has services of HIV blog, forum, ask HIV counselor, real poz inspirational stories and other general dating. The network has been helping HIV... Read more »
Poz Gay, Hiv Positive Gay Men and Bisexual Find gay with hiv, HIV Gay Dating, HIV Gay
Posted by Abivichttp://www.PozBuddies.com focuses on providing a safe Poz Match & Support group for HIV Personals. It has services of HIV blog, forum, ask HIV counselor, real poz inspirational stories and other general dating. The network has been helping HIV... Read more »
Poz Gay, Hiv Positive Gay Men Find gay with hiv at PozGays.com
Posted by Chloe1023Over 20% gay and bisexual men are living with hiv positive in the U.S. alone. Are you one of them? If you are HIV Gay or Gay men with herpes it can make you feel like you are all alone in the world. Do you wish there was a place where you didn't... Read more »
Ask the Experts
dating my first positive gay manHi,iam a 45 yrd old man,who just started having a relationship with a gay hiv+man.My problem is i felt more anxiety than i thought i would,during sex he ejaculated on my bare chest,i was alittle upset.What are the chances of transmitting the...
Response from Robert H. Remien, Ph.D.
Columbia UniversityRisk of transmission of HIV from the behavior you describe is extremely low (see the forum on Safe Sex, HIV Prevention and Transmission on the website of The Body). Anxiety when having sex is not fun and needs to be addressed. You and your partner... Read more »
gay sero-discordant relationship (MAGNETIC COUPLE PROBLEMS) (SHOULD I DUMP MY POSITIVE LOVER)Hi,My bf is HIV+, I am not. I thought I could handle the pressure but Im thinking again. I dont know. He is a great guy and would be "the 1" in an ideal world. I read the archives and understnd you are in a happy magnetic relationship,...
Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS FoundationHello,Let's deal with your tonsillitis and testing issues first and then turn to your relationship concerns, OK?First, why assume the tonsillitis is viral? It could very well be bacterial. I would suggest you have your general medical doctor... Read more »
dated someone who was HIV positive...Dr. Frascino,Quick question. I dated a guy for a couple of months involving physical intimacy. I found out six months later that he was HIV positive. Obviously I should get tested. However, I understand that even exposed to HIV, depending upon...
Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS FoundationHello,It's alarming that you are having sex, yet know so little about HIV. I can only assume Dubya's disastrous "abstinence-only" sex education policy has struck once again! Or perhaps you were home schooled by folks who believe... Read more »
Gay HIV+ partner has bareback sex with other HIV+ peopleHi, I'm in an open gay relationship with my HIV+ partner whereas i am HIV-. We always have protected anal sex (oral unprotected)My partner continues to have unprotected sex with other positive guys even though i have stressed that this is bad....
Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS FoundationHi,Regarding the risk of poz-on-poz barebacking, see below.As for a well "recegonised" resource on this topic, HELLO! You're reading it. Check out the archives! Finally, regarding the risk of STDs from oral sex, we have an entire... Read more »
Gay Dating App Hornet Doesn't Leave HIV BehindNowadays, with a social network for any and every interest, using these networks has become the norm. Gay social networks, aka "dating" apps, have replaced their predecessors, online websites, and have taken over as one of the more popular ways for... Read more »
Dating Resources: Can You Still Find Love and Companionship When You Are HIV Positive? The Answer From Our Panelists? A Resounding Yes!The following contains resources you can use to get out there! U.S. Dating Sites for HIV-Positive Gays or Straights Positive Love Free to join HIV Passions Free PozMatch.com Fees charged for most features Planet POZ Personal ads... Read more »
Positively Sexy: Volttage Provides an Online Space for Dating and ExpressionFor HIV-positive gay men, the dating world can be a difficult forest to navigate. In a community that can be heavy on the positive stigma, having to divulge your status and wait for the other guy to "be OK" with it can get old really fast. Enter... Read more »
Ask Danny: Dating Advice for Gay MenWho should make the first move? How do I tell if he has a boyfriend? When do I disclose that I'm HIV-positive? Where can I go to meet Mr. Right besides a gay bar, and how will I tell the difference between Mr. Right and Mr. Right Now? As a... Read more »
Guides for People With HIV


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A leading figure with the Muslim Brotherhood's Freedom and Justice Party projected the party winning 55 percent of seats in the House of Representatives during the next elections in April.
Ibrahim Abu Auf of the Freedom and Justice Party told Al-Masry Al-Youm that the party could win "perhaps even more seats, if the party forms coalitions with other political forces."
Abu Auf's statements echo those of other party officials, underlining the party's confidence of achieving a majority in the next Parliament.
The FJP emerged as the strongest political party in the country after the 25 January revolution overthrew longtime President Hosni Mubarak and his National Democratic Party. Founded by the Muslim Brotherhood, the FJP won around 42 percent of the seats in the lower house of Parliament, the now-dissolved, People's Assembly, in the first elections after the revolution.
However, some commentators say that the party will face a stiff challenge in the next elections from both secular and ultra conservative Salafi parties.
The main secular coalition force, the National Salvation Front, said that they will run for elections with a unified electoral coalition. And on the Salafi side, former Nour Party head Emad Abdel Ghafour's new Al-Watan Party has forged an alliance with popular preacher and former presidential candidate Hazem Saleh Abu Ismail.
However, Abu Auf said that such changes in the political landscape ahead of parliamentary elections are not a source of concern for the FJP.
"We have three times more candidates than needed," he said, adding that voters will not be swayed by other parties attempts to tarnish the image of the Brotherhood.


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The staff was very nice and the food was cooked to order. The Restaraunt is not under the restaurant section of trip advisor at this writing but it was one of the best. It is no longer German but Peruvian and the fish chowder that I had was great.
- Also Known As:
- Hotel Los Capitanes Eco-Resort Panama/El Valle De Anton


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Edwin van der Sar The only worry at 40 years of age is what such a lack of movement and action in this cold weather does to his limbs (only joking Edwin!). The fans sang his name throughout the second half.
John O'Shea Can be pleased with his performance. Didn't have anything to do defensively, but chipped in in attack, including the flick-on that got the scoring started.
Chris Smalling Calm and assured but, again, this was an easy afternoon defensively.
Nemanja Vidic Cleared anything aerially in the first half, then just sat back and enjoyed the attacking show like the rest of us.
Patrice Evra Went off at half-time, but he was a threat in the first half going forward .
Nani He's not quite produced 2010's form in 2011; but he was a constant threat down the right flank. Kept probing even when his final ball didn't come to anything and he finally got a goal.
Michael Carrick It was a shame that injury forced him off so early in the game. He was running the midfield.
Anderson Full of life and energy, he was the bedrock of a rampantly attacking United display. He's at his best and says there's still more to come – a frightening thought.
Ryan Giggs There's just no stopping him is there? Constant menace whether it was out wide on the left or pulling in-field. He came off after less than


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For a 2-14 team, the Texans should be plenty newsworthy over the next few months. They have a head coaching search that should officially conclude with an announcement on Thursday that Bill O'Brien is coming to Houston, and they have the number one overall pick in the 2014 NFL Draft, which should ... More >>
We are at the mathematical halfway point of the Texans season, and the team sits at 2-6. It's funny how this weekly BATTLE-DRINK game, if you go back and look at each week, in an odd way sort of weaves the tapestry of the entire season. You can see how all the storylines and futility have evolved we ... More >>
Here's me channeling an NFL player... After a September and October that included two Texans road trips, three Texans home games, a trip to College Station for Alabama-A&M, and a trip to South Bend for Oklahoma-Notre Dame, the bye week came at a good time for me. Yes, that's right, last weekend's ... More >>
Full disclosure: I've never sifted through someone else's garbage, and I've never acquired any piece of my furniture by snagging it from the end of someone's driveway after they've thrown it out. But to each their own, and if you've done this before, hey, as Tony Soprano would say, "Ya do whatcha ... More >>
On Tuesday, the Texans made their final set of moves to get down to 75 players before they head up to Dallas on Wednesday for the game Thursday night, placing wide receiver Alec Lemon on the injured reserve list and placing nose tackle David Hunter and wide receiver Mike Smith on waiver/injured rese ... More >>
They say the third preseason game is the one that's supposed to most closely resemble a regular season game. With most of the starters (the available ones, at least) playing a full half, the game against the Saints Sunday was supposed to give Texan fans a decent glimpse at what's in store for 2013. ... More >>
Was preseason football fever in full swing on Saturday? Well, late Saturday afternoon, I managed to make it from my home near downtown to the area near Reliant in almost record time, thanks to very light Saturday traffic. As I drove down Murworth to the media parking area, roughly two hours before ... More >>
He made millions sculpting the figures of Houston women while dodging one of the largest malpractice judgments in Texas history. But after botching another surgery, can Dr. Gerald Johnson continue to operate?


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We are a substance abuse treatment facility that is developing an EMR software to improve our clinical and business outcomes. We have back end developers working on the system in asp.net and we need h…
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This company is a start-up. We have developed an online platform that can revolutionize the way things are done in a large niche market - think disruptive. We are at the tail end of developing our Bet…
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ReceptionIt: Where the party starts. At ReceptionIt.com we are revolutionizing the way people planning events find, compare and book the perfect event space for any occasion. ReceptionIt is an innovat…
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Diamond Star Media, LLC — Providence, RI
We are looking for Web Designer/ Developer interns, we are looking for interns who are cutting edge and not afraid to use all the new cutting edge designs ways while also understanding traditional va…
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We're a small team of hipsters, hackers and hustlers working together to make the web a better place. We specialize in web design, development and branding. We love working with startups and companies…
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The City of Charlotte Shared Services Technology Management is seeking three interns with experience in Process Improvement and Technical Writing. Experience gained in the classroom will be accepted. …
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Content Management Intern Job Description: Nutrisystem, a leading provider of weight loss, is actively seeking a motivated, technically savvy, and detail oriented individual to operate in a fast paced…
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Crabtree & Eveyn, Ltd — Woodstock, CT
Crabtree & Evelyn was founded in 1972 by Cyrus Harvey. Inspired by the quintessential English country garden, the early home apothecary and pioneering British explorers, this young company was among t…
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Progressive Solutions is a small IT consulting company that manages over 20, small to medium sized companies. We help recommend, purchase, install and maintain all facets of our clients server and com…
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Instant Customer — San Diego, CA
We are an Internet Marketing Software company in Old Town San Diego, and we are looking for 1 intern to help us with IT tasks including hardware and software selection. PHP programming knowledge a PLU…
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24 year old gay vegan working on my second degree in environmental horticulture at Virginia Tech.


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Word: giovanni santoriello
Added on: 2009-10-31 Listened: 74 timesin: italian, pasta, pizza, Reavirri, Roma, Cissili, SmallCock, gayboi, fagoti, strongman
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giovanni santoriello = john Santoriello
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Select () or exclude () categories to narrow your recipe search.
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1/5 Photos of Spicy Sushi Sauce
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This is Japan's answer to McDonald's special sauce. Is this what you get with your sushi at a Japanese restaurant? If not, it shoud be! Adapted from Joni Marie. She has some great and creative recipes.
Units: US | Metric
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sriracha sauce
Every recipe, menu, tip and how-to is right here, at your service.


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When Adidas created the Adidas Champion BJJ Gi , they set out to create the best competition GI possible and did just that with the development of the IBJJF approved competition uniform "Champion" model. Competition ready this GI is perfect for both competing or daily training! Lite, durable and a preshrunk (weave) made of a soft cotton-poly blend. The pants are preshrunk 60/40 cotton-poly blend rip stop pants. Direct Embroiders at pants and jacket. Comes with free adidas Satin carry Bag!
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Excellent Quality
By Mifune
from Los Angeles
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Comments about Adidas Champion Gi:
This is a great gi. Made perfect for BJJ. The quality is top notch and for a brand like Adidas it's got a great value. I've had Atama, Koral and SYR, this is my favorite
Bottom Line Yes, I would recommend this to a friend
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Photos and Description of this 3 bedroom holiday apartment in Cubelles - Voramar
Property details
building and outdoor:
Vilanova i la Geltrú: Child friendly, comfortable house Voramar, built in 1975, renovated in 2013. Paseo Marítimo, in a sunny position, 15 m from the sea. For shared use: garden. Outdoor shower. Private: terrace (10 m2). Stepped path to the house. Parking. Grocery 200 m, supermarket 2 km, restaurant 200 m, bar 400 m, bus stop 200 m, railway station 700 m, sandy beach 15 m, swimming bay 300 m. Golf course 5 km. Nearby attractions: Barcelona centro 50 km, Sitges 8 km, Tarragona 50 km. Please note: railway line 700 m from the property.
4-room apartment 70 m2 on 1st floor, fully renovated in 2013, comfortable and modern furnishings: living/dining room with TV. Exit to the balcony, to the terrace. 2 rooms, each room with 1 bed (90 cm, length 200 cm). Exit to the balcony. 1 room with 1 double bed with fan. Kitchen (oven, dishwasher, 3 ceramic glass hob hotplates, freezer) with dining table. Shower/WC. Boiler (50 litres). Balcony 5 m2, terrace 10 m2. Terrace furniture. View of the sea. Facilities: washing machine, iron. Reserved parking. Licence number: HUTB008831
- Suitability:
- pets not allowed


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Founder of @HomeHero and previously @Flowtab. Bona fide aesthete and triathlete. You can find me at @ScienceInc.


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Date February 29, 2008 at 11:00 PM
Categories Health & Beauty
Forget for a moment the long stretch of cars outside on NM 528. Ignore Intel's industrial bulk next door. Block out the vision of the Sandia Mountains looming to the east. Instead, when you walk into Venezia's New York Style Pizzeria, close your eyes and inhale. You could be in the Bronx, or even Little Italy. Because that smell is New York style pizza at its finest.
Order a slice. See that crust, how it's hand-tossed, puffy at the edges, and tapers to a thin, crisp middle? That's how you make a pizza crust. The sauce is of course made fresh each day with nearly a dozen different spices, the exact mix a closely-held family secret. As for the toppings, you can order from almost twenty different kinds, but if you're a purist, you'll stick with plain cheese. And don't even think about using a knife and fork. Just fold the slice in half and eat it already.
If you think about it, it's not much of a stretch to assert that some of the best pizza in New Mexico is located in a strip mall in Rio Rancho. Say what you will, Rio Rancho has nonetheless represented the epitome of the American Dream for hundreds of families-many of them Italian-American-who migrated here in the 1960s and 1970s.
Families like Aldo and Renato Venturino's. Lifelong residents, the brothers are also owner-operators of the most recent incarnation of Venezia's, a Rio Rancho fixture since 1978.
"My parents came over to America from Italy in their early twenties," says Aldo. "They loved Italy, but it's a poor country and they wanted a better future."
While the family, which included Aldo's parents, Maria and Adolfo Venturino, and his aunt and uncle, Clara and Frank Montanile, started out in New York City, they eventually made their way west in the early 1970s. "It was all those AMREP brochures," Aldo says, laughing at the memory of the controversial development company's questionable sales tactics. "The brochure showed all these photos of green grass, but when we got here there was nothing but desert."
Still, for many families, Rio Rancho was a paradise. "This was a place where you could really afford to make a new start," he continues. "For instance, you could afford to buy a home and the taxes were cheap. People back east, they couldn't believe how low the taxes were."
AMREP may have shined it on a little thick, but those who took the gamble literally built a city from the ground up. Rio Rancho was, and still remains, a place that rewards know-how and ambition. Maybe not in the lavish style of, say, a Donald Trump, but most certainly in a way understandable to the average American: the opportunity to work hard, to start a business, to produce a product or service that makes one proud.
In that spirit, Aldo's uncle Frank opened the city's original Venezia's, which thrived until 1995 when Frank and Clara moved to Arizona to be near their son and daughter.
In 2000, Aldo and Renato decided to reopen the restaurant. While uncle Frank has since established the Venezia's name in Chandler, Tempe, and East Mesa, Arizona, he happily lent his support and today serves as a silent partner in the New Mexico operations, which also include three Albuquerque locations.
"Doing this was a dream come true for us, just like it had been for our uncle," says Aldo. "My family, we came from nothing, we had nothing when we started out here. But we made something. My brother and I were talking about this, about our future, and this is what we decided to do."
But why the restaurant business? Between the hours, the regulations, the food and labor costs, it seems a downright daunting way to make a living. "If working in a restaurant is hard," Aldo concedes, "owning it is probably five times harder. But it's like my uncle said, you know you always have a job. Times get tough, you work harder and put more in to it and you'll always be able to pay your bills. We're not looking to get rich. We're looking to make a living and to give our customers a quality product. That's what this business is about-the passion of doing something well."
And if Aldo is passionate about anything, it's pizza. "To me, New York style pizza means pizza made with love. It's one thing to roll out some dough and toss on some sauce and a few toppings. It's another to carefully hand toss your crust, to make sure its edges are even, to make fresh sauce every day, to use the best toppings and spread them evenly on the pizza, from middle to the edge. I want my customers' last bite to be as good as their first. That's how I express it. Sure, it may only be pizza, but some people love pizza more than anything, right?"
In addition to four sizes, including a 24" party size from which the individual slices are served, and seven specialty combos, Venezia's also serves a variety of salads, calzones, hot and cold heroes and subs, and pasta dishes, all made fresh each day and presided over by Venezia's original cook, Aldo and Renato's mother, Maria. "She works six to seven days a week and makes everything from scratch," Aldo says proudly. "To her, there's no difference between these kitchens and her home kitchen. So she picks out only the best meats, the best chicken, the freshest produce."
Just as the brothers feel obligated to give their customers the best food possible, so too do they feel a sense of duty to their community. The walls are hung with commendations from local high school athletic departments, the Rio Rancho Fire Department, and UNM Hospital's Air Emergency Service, to name a few, all thanking Venezia's for its assistance.
"We feel very privileged to be able to give back to the community," explains Aldo. "We grew up here; we saw the city grow into what it is today. But to me, it will always be a small town where everybody knows each other, a good, safe place where I can raise my family."
And continue its legacy. "It's pretty simple where we're coming from: we want to make a living and we want to please our customers. We're honest, we're fair, and if we make a mistake, we fix it. That's how we were raised. That's who we are. Old-fashioned Italians, living the American dream."
Venezia's offers delivery, catering, and dine-in at three locations:
1690 Rio Rancho Blvd. SE, Suite E
Rio Rancho
3908 San Mateo Blvd. NE
1331 Juan Tabo Blvd. NE, Suite 2-F


text: Maria Ozawa is a kinky slut who likes to face sit on a man's face before she jumps on his long cock. She also loves to squirt directly in a dudes mouth as she is having an orgasm or to splash his face with lots of pussy juice. Maria Ozawa is a cunt who goes rough on her fuckers.


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i need more than 160 letters bro.... model&racekarts _____Marcus'Doore. 27.09.123 ______Sure, there are other fish in the sea, but you'll always be my nemo.