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Lake Buena Vista hotels
Visit Lake Buena Vista
Home to one of the world's most beloved theme parks, Lake Buena Vista is visited by millions of travelers each year. Shop, dine and play at Walt Disney World, and enjoy the fun accommodations. Book Lake Buena Vista hotels through Orbitz.com, where you can find deals and low prices.
Disney Park Area
Walt Disney World is visited by more than 17 million people each year. With four main parks, two water parks and plenty of dining and shopping options, this resort has something for travelers of all ages. The Clarion Inn is located on Palm Parkway, less than 15 minutes from the resort. The hotel has two pools, a gift shop, a playground, laundry facilities and an on-site restaurant, making it an ideal choice for business travelers as well as families. A free shuttle is available to all four main theme parks, and some pets are allowed. Rooms average $56 a night, and the hotel can be reached at (800) 999-7300. Staying on Disney property is convenient and fun, and there are many options to delight families and Disney fans. The All-Star Music Resort is one of the more affordable choices. With an on-site gift shop, food court, pool and arcade, this hotel is a popular choice with visitors. Those traveling with a group can book one of the roomy family suites, and all rooms are decorated with a fun music theme. Rooms average about $75 per night. Call the hotel's front desk at (407) 934-7639 for details. Disney's Grand Floridian Resort & Spa offers more luxurious on-site accommodations. This Victorian-themed hotel has a beach, a pool, a spa, a gift shop and dining options to delight travelers. The resort monorail passes through the hotel, and bus transportation is available to all of the park's main attractions. During the winter holiday season, the hotel is a sight to behold. Each year, a giant gingerbread house is constructed in the lobby, and the entire building is given a Christmas makeover. The theme parks host special parades and events, making December one of the most popular months to visit.
Downtown Area
Known for its diverse dining and shopping establishments, the Downtown Disney area has many attractions to enjoy. Give standing ovations to the Cirque du Soleil show La Nouba, or visit DisneyQuest and explore five floors of interactive video games and entertainment. The Royal Plaza hotel has a fitness area, pools, lighted tennis courts, on-site dining and luxurious pillow-top mattresses. Choose a Jacuzzi room to relax those tired muscles after a long day of sightseeing, or book an in-room massage. Take a free shuttle to one of the Disney theme parks, or tee off at one of the city's championship golf courses. Rooms average $69 a night, and you can call (407) 828-2828 for more information.
Experience Lake Buena Vista
Whether you are headed to sunny Florida for a family reunion, annual vacation or business trip, Lake Buena Vista is a traveler's paradise. Enjoy the sights and sounds of one of the world's favorite theme parks, or enjoy a day of dining and shopping. No matter when you plan to visit, Orbitz.com can help you find Lake Buena Vista hotels that meet your needs and budget.
Explore other Lake Buena Vista hotels by:
Lake Buena Vista hotels by star rating
- 5 star hotels (1 hotel)
- 4 star hotels (25 hotels)
- 3 star hotels (16 hotels)
- 2 star hotels
- 1 star hotels
Travel to Lake Buena Vista
Recently booked Lake Buena Vista hotels
|Last booked
|Price from
|WyndhamVR Bonnet Creek Resort
|26 minutes ago
|Wyndham Lake Buena Vista Resort
|42 minutes ago
|Disney's Beach Club Resort
|52 minutes ago
|B Resort & Spa - Lake Buena Vista
|1 hour ago
|Disney's Pop Century Resort
|10 hours ago
|Disney's Coronado Springs Resort
|10 hours ago
|Walt Disney World Dolphin
|11 hours ago
|Legacy Vacations Resorts - Lake Buena Vista
|12 hours ago
|Holiday Inn ORLANDO - LAKE BUENA VISTA
|12 hours ago
|Disney's Port Orleans Resort - French Quarter
|12 hours ago
Recently Rated Lake Buena Vista Hotels
|Price from
|B Resort & Spa - Lake Buena Vista
4.3 / 548 reviews
|Disney's Port Orleans Resort - French Quarter
4.1 / 5153 reviews
|Disney's Pop Century Resort
3.9 / 5539 reviews
|Wyndham Lake Buena Vista Resort
3.3 / 51424 reviews
|Disney's Old Key West Resort
3.9 / 516 reviews
|Disney's Art of Animation Resort
4.1 / 5115 reviews
|Disney's Port Orleans Resort - Riverside
4.0 / 5236 reviews
|Disney's Yacht Club Resort
4.4 / 533 reviews
|Legacy Vacations Resorts - Lake Buena Vista
4.1 / 568 reviews
|Walt Disney World Dolphin
3.9 / 5455 reviews
Activities in Lake Buena Vista
Universal Orlando Theme Parks
SeaWorld Orlando
Busch Gardens Tampa
Wet 'n Wild
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Featured Lake Buena Vista Hotels
1 B Resort & Spa - Lake Buena Vista
1905 Hotel Plaza Blvd. Map
B Indulged Spa Special Offer
Elegant rooms and casual dining in the heart of Downtown DisneyB Resort and Spa, an Official Walt Disney World Hotel, is a lifestyle resort near the Downtown Disney area. The resort features... More
Great room!
2 Legacy Vacations Resorts - Lake Buena Vista
8451 Palm Parkway Map
Take 30% Off Your Stay
Our resort puts you a stone's throw from all the Orlando excitement. Situated in the midst of the world's most popular attractions, you'll feel the magic the moment you arrive. Our grand villa... More
3 Clarion Inn Lake Buena Vista, a Rosen Hotel
8442 Palm Pkwy. Map
All rooms have coffee maker, refrigerator, and microwave. FREE scheduled shuttle to Magic Kingdom, EPCOT, Animal Kingdom, MGM Studios, Universal Studios, Islands of Adventure, and Sea World. Gift... More
Good value, clean room
4 Buena Vista Palace
1900 Buena Vista Dr Map
Includes $75 USD Dining Voucher, Spa Discount
Downtown Disney resort offers golf access, Disney character breakfastsLocated inside Walt Disney World Resort, Buena Vista Palace provides a top-notch Disney experience for both kids and grown-ups.... More
Great, except the bathroom smelled like Hotel 6.
5 Disney's Pop Century Resort
1050 Century Drive Map
Disney Resort Benefits
Disney groups hotel properties into three categories: value, moderate and deluxe. Pop Century is a value resort, great for families on a budget. Themed around trends spanning the 1950s through to... More
Fantastic family getaway
6 Holiday Inn ORLANDO - LAKE BUENA VISTA
1805 Hotel Plaza Boulevard Map
No Resort Fee, Kids Stay & Eat Free
The only Holiday Inn hotel in the WALT DISNEY WORLD® Resort With No Resort Fee , our unique Walt Disney World® location makes this the perfect place to stay during your next Orlando family... More
Nice clean hotel
7 Disney's Art of Animation Resort
1850 Animation Way Map
Disney's Art of Animation is all about getting behind the scenes, with rooms themed around animated favorites like The Lion King, The Little Mermaid and Finding Nemo, and bedspreads and murals that... More
Decent Disney Resort
8 Walt Disney World Dolphin
1500 Epcot Resort Boulevard Map
Save 10 % on your stay
OverviewJust south of the Magic Kingdom, you'll discover a resort that enchants children and grownups alike. Florida hotels promise deluxe accommodations and this remarkable Disney property... More
Perfect Disney Location
9 Wyndham Lake Buena Vista Resort
1850 Hotel Plaza Blvd. Map
The Wyndham Hotel Corporation was started in 1981, and today the company has grown into a recognized name that provides upscale service in popular destinations throughout the world. The Wyndham... More
Great location
10 Hilton Orlando Resort Lake Buena Vista
1751 Hotel Plaza Boulevard Map
Rate includes breakfast and internet access
Hilton Orlando Lake Buena Vista, located in the Walt Disney World® Resort, is Hilton's only hotel with Extended Theme Park Hours. This exceptional Lake Buena Vista hotel is ideally situated next to... More
Love it! But..
11 Walt Disney World Swan
1200 Epcot Resort Boulevard Map
Save 10 % on your stay
OverviewDisney's Swan Hotel is famed for its modern design and touch of elegance – the Florida hotel was designed by award-winning architect Michael Graves. Allow yourself to be transported to an... More
Annual pleasure
12 SPM Blue Tree Resort at Lake Buena Vista
12007 Cypress Run Dr. Map
Relax in your own 1 or 2 bedroom suite, complete with all the amenities, perfect for business travelers, couples, and families looking for the ultimate in serenity and comfort. Blue Tree Resort... More
13 Disney's All-Star Music Resort
1801 West Buena Vista Drive Map
Disney Resort Benefits (Including ticket info)
Oversized replicas of cowboy boots, guitars and saxophones decorate the music-themed exterior of Disney's All-Star Music Resort. Designed with Disney's signature knack for themed attractions, hotel... More
Anniversary Stay
14 Disney's All-Star Movies Resort
1901 West Buena Vista Drive Map
Disney Resort Benefits
Here's the perfect place for you if you need to make your vacation dollars last and last - Disney's All-Star Movies Resort! And guess what? You'll feel like a kid again when you stand next to the... More
Affordable Last Minute Destination - Great Staff
15 Fairfield Inn & Suites Orlando Lake Buena Vista
12191 South Apopka Vineland Road Map
As an Official Walt Disney World Good Neighbor® Hotel, the Fairfield Inn & Suites by Marriott® gives you confidence that your trip will be a success because we consistently deliver a hotel... More
16 Disney's Port Orleans Resort - Riverside
1251 Riverside Drive Map
Disney Resort Benefits
Return to the charming days of riverboats and Mark Twain as you discover this moderately priced resort. Courtly pillars, grand staircases and splendid gardens blend with the Mississippi-style... More
Fantastic Disney Vacation Location
17 Westgate Blue Tree Resort at Lake Buena Vista
12007 Cypress Run Dr. Map
Fall Sale, Save 20%
Nestled on 23 tropically landscaped acres, Westgate Blue Tree Resort lies just 3 miles from Walt Disney World Resort, 5 miles from Sea World Orlando and 7 miles from Universal Orlando. This... More
family vacation
18 Doubletree Guest Suites Resort Lake Buena Vista
Walt Disney World, 2305 Hotel Plaza Map
Rate includes Breakfast for 2 adults & 2 children
All-suite official World Disney hotel with free shuttle The Doubletree Guest Suites Resort Lake Buena Vista is located inside Downtown Disney, offering guests more than just a comfortable stay.... More
Good value hotel
19 Disney's All-Star Sports Resort
1701 West Buena Vista Drive Map
Disney Resort Benefits
Here's the perfect place for you if you need to make your vacation dollars last and last - Disney's All Star Sports Resort! And guess what? You'll feel like a kid again when you stand next to the... More
Great Disney Value Resort!
20 Disney's Port Orleans Resort - French Quarter
2201 Orleans Drive Map
Disney Resort Benefits (Including ticket info)
Intricate railings, cobblestone streets and courtyards of flowers capture the spirit and romance of New Orleans' French Quarter. Dine on Creole delicacies, plunge into Doubloon Lagoon with its... More
Disney Vacation
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Slide 7 | Obamacare in Pictures
Between the recession's low point in January 2009 and April 2010, net private-sector job growth improved at a rate of 66,800 jobs per month. After Obama signed his health care legislation into law at the end of March 2010, the improvement stalled. Over the next 29 months, private-sector job growth improved at a rate of just 300 jobs per month.
View these easy to understand charts about the impact of Obamacare.
Read more reports on the Impact of Obamacare.
The battle for the future of Ukraine rages on. Despite a ceasefire negotiated in Minsk in September, in Donetsk and Luhansk in Eastern Ukraine, separatists — Read more
Whistleblowers, Leaks, and the Media is a broad-based introduction to the legal and policy issues regarding the media's coverage of national security. A joint — Read more
The current tax system imposes high marginal tax rates on work, and its treatment of savings and investment is destructive. The current system distorts economic — Read more
Many history professors have allowed their biases to distort the way America's past is taught, searching for instances of racism, sexism, and bigotry in our — Read more
Five years ago, Rick Santelli's unscripted remarks on the floor of the Chicago Board of Trade catalyzed a grassroots upsurge of popular discontent directed at — Read more
Although Alexis de Tocqueville is frequently quoted, his advice is rarely taken. Tocqueville has much to teach us, not only on how to judge our — Read more
According to NPR's Planet Money: "If you want to send a bunch of oranges by truck from Florida to Baltimore, no one cares who — Read more
FeaturingThe Honorable Jim TalentFormer United States Senator from Missouri and Member of the Senate Armed Services Committee;and Member, National Defense PanelHosted byThe Honorable Jim DeMintPresident, — Read more
The federal minimum wage prohibits Americans from volunteering for entities organized as for-profit businesses? As a result many family businesses and community organizations have found — Read more
8:30 a.m. - Registration and Continental Breakfast 9:00 a.m. - Welcome and IntroductionAlden Abbott, Deputy Director, and the John, Barbara, and Victoria Rumpel Senior Legal Fellow,Edwin Meese III — Read more
The Heritage Foundation is the nation's most broadly supported public policy research institute, with hundreds of thousands of individual, foundation and corporate donors. Heritage, founded in February 1973, has a staff of 275 and an annual expense budget of $82.4 million.
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Oprah Winfrey: 'Running A Network Is Challenging'
- Photo: Ben Rose/WireImage1 of 16
- Photo: Evan Agostini/Invision/AP2 of 16
- Photo: George Burns/OWN/Invision/AP3 of 16
- Photo: John Amis/Invision/AP4 of 16
- Photo: Chris Pizzello/Invision/AP5 of 16
- Photo: Evan Agostini/Invision/AP6 of 16
- Photo: SGranitz/WireImage.com7 of 16
- Photo: POA/ARB/WENN8 of 16
- Photo: caplakesting.com/Splash News9 of 16
- Photo: Julie Jacobson/Invision/AP10 of 16
- Photo: Jackson Lee/Splash News11 of 16
- Photo: Themba Hadebe/Invision/AP12 of 16
- Photo: Charles Rex Arbogast/Invision/AP13 of 16
- Photo: Charles Rex Arbogast/Invision/AP14 of 16
- Photo: RG4/WENN15 of 16
- Prev
Does Oprah Winfrey have buyer's remorse trading in her iconic talk show for an entire network bearing her name? Not quite.
The media legend, speaking with protégé Dr. Mehmet Oz Wednesday, admitted that while she misses aspects of her former career, she's still focused on making her OWN network take off after a year of sagging ratings.
"I don't miss doing the show everyday ... I miss the audience, I miss the people," she said, noting how she loved connecting with the masses after the cameras were turned off.
PHOTOS: Oprah Gets Star Studded Farewell Surprise!
As for her network, the veteran broadcaster told Dr. Oz, "Let me tell you: Running a network is challenging ... can y'all please find it on your channels?"
Undaunted by the OWN network's tepid first-year ratings, she said, "You have to be committed because, you know, I signed a deal. I'm going to do it."
She appeared on the show to promote the January issue of her O magazine, which features a cover of she and Dr. Oz, the first man to be featured on the front of the magazine. The only other people Oprah's ever joined on the cover are First Lady Michelle Obama, and talk show hosts Ellen Degeneres and Rosie O'Donnell.
During the final show of her 25-year run in May, Oprah told her studio audience, "I thank you for being as much of a sweet inspiration for me as I've tried to be for you.
PHOTOS: Oprah: Through The Years
"I won't say goodbye: I'll just say, until we meet again ... to God be the glory," leaving the stage to the theme of Paul Simon's "25 Years."
Fun fact: Oprah's name is listed on her birth certificate as "Orpah," but she changed the spelling because people had difficulty pronouncing her name, according to iMDb.
Watch the video on RadarOnline.com
Regis & Kelly, Ellen & Dr. Oz Among Big Winners At Daytime Emmys
Oprah: 'I Have A Dream Of OJ Confessing To Me'
Oprah Winfrey's Farewell Scores Her Highest Ratings In 17 Years!
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Impact of sequester on states detailed in new White House reports
- Written by The Michigan Chronicle
- Published in Detroit
WASHINGTON -- It's looking increasingly like the government is about to impose on itself $85 billion in across-the-board spending cuts. So as part of its strategy to pressure Republicans into accepting new revenues as part of a deal to prevent the sequester from taking effect, the White House released new reports on Sunday that outline the ugly effects those cuts would have in individual states.
Read more at http://www.huffingtonpost.com/2013/02/24/sequester-states_n_2755181.html?utm_hp_ref=detroit&ir=Detroit
Read more http://www.michronicleonline.com/index.php/2011-08-04-18-06-26/rss-feeds/180-news-briefs/9805-impact-of-sequester-on-states-detailed-in-new-white-house-reports


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- Queensland is full of colourful characters - those who make the headlines and others who don't. In this 12-part series, we'll bring you some of the lesser known, but equally important, faces and stories of the Sunshine State....Wednesday 19 February 2014
- Sunday 16 February 2014
- Organisers of a Valentine's Day campaign to send home-baked biscuits and cakes to drought-affected families in western Queensland say it will be an ongoing project....Friday 14 February 2014
- Sunday 26 January 2014
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- More than 2,000 students from across Queensland will test their endurance in a 24-hour human-powered vehicle race in Maryborough in the state's south-east this weekend....Friday 13 September 2013
- The organisers of the Mary Valley Scarecrow Festival near Gympie in south-east Queensland say billionaire Clive Palmer's look-alike could make an appearance this year....Thursday 12 September 2013
- More than 100 aged care residents have returned home, eight months after floods inundated a nursing facility in Bundaberg in southern Queensland....Thursday 5 September 2013
- Up to 500 motorcyclists will this week travel more than 2,000 kilometres across central Queensland to raise funds for spina bifida research. ...Monday 2 September 2013
- Sunday 18 August 2013
- A commemoration service has been held on a picturesque potato farm outside Bundaberg in southern Queensland, honouring dozens of people buried in unmarked graves....Friday 16 August 2013
- "We're really lucky in Australia. We're lucky that there can be a discussion and a debate, and we can find out information to make an informed choice." Petrina McDonald works as a high school teacher in Bundaberg North. She is passionate about education and the arts, and believes Australia needs ...Thursday 8 August 2013
- A camel racing enthusiast has called for more training for jockeys after the death of a rider in Winton in central western Queensland. ...Thursday 1 August 2013
- Rural Woman of the Year Alison Fairleigh says farmers are feeling powerless and it is affecting their mental health....Tuesday 23 July 2013
- A small column of smoke rises from the forge as the handle spins to bring it up to speed. The tapping sound of hammer on steel can be heard at many markets and shows around the Wide Bay in Queensland. ...Thursday 27 June 2013
- A band from Gympie is causing movement on the local music scene with their talent and original sound....Wednesday 26 June 2013
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- The parents of murdered Queensland teenager Daniel Morcombe say they are proud their child protection work has been recognised in today's Queen's Birthday honours list....Monday 10 June 2013
- Frances Adcock is a News Journalist for the Wide Bay Burnett region. ...Friday 7 June 2013
- The Mary Valley Heritage Railway at Gympie in south-east Queensland says it needs up to $2 million to repair damaged tracks and get the Rattler steam train fully operational again....Thursday 30 May 2013
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Town Square
Menlo, Castilleja teams meet in CCS
Original post made on Jul 3, 2007
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on Jul 3, 2007 at 12:51 pm
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Miley Cyrus Bong: For Sale!by Hilton Hater at . Comments
A Miley Cyrus CD? $15.
Miley Cyrus concert tickets? $50.
The bong from which Miley Cyrus smoked salvia and created an Internet firestorm? Priceless. Maybe.
According to TMZ, the guy who owns the pipe that got this singer into trouble has decided to sell the item.
Believing he can get close to "five figures" for the drug paraphernalia, the owner has set up a Gmail account (mileycyrusbong) and is entertaining offers as we type. This forces us to ask: How much would you pay for Miley's bong?
why don't yall get the fuck ovet it iam so tired of hearing about this i don't care for miley anyway she's a loser and she is going end up like lisdnay lohan.


text:
Article first published online: 19 NOV 2010
DOI: 10.1111/j.1600-0897.2010.00931.x
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Special Issue: Sexual Transmission of HIV in the 21st Century
Volume 65, Issue 3, pages 292–301, March 2011
Additional Information
How to Cite
Doncel, G. F., Joseph, T. and Thurman, A. R. (2011), Role of Semen in HIV-1 Transmission: Inhibitor or facilitator?. American Journal of Reproductive Immunology, 65: 292–301. doi: 10.1111/j.1600-0897.2010.00931.x
Publication History
- Issue published online: 7 FEB 2011
- Article first published online: 19 NOV 2010
- Submitted October 14, 2010; accepted October 18, 2010.
- Abstract
- Article
- References
- Cited By
- HIV transmission;
- inflammation;
- mucosal immunity;
- semen;
- vaginal cells
Citation Doncel GF, Joseph T, Thurman AR. Role of semen in HIV-1 transmission: inhibitor or facilitator? Am J Reprod Immunol 2011; 65: 292–301
Sexual transmission of human immunodeficiency virus type 1 (HIV-1) accounts for 60-90% of new infections, especially in developing countries. During male-to-female transmission, the virus is typically deposited in the vagina as cell-free and cell-associated virions carried by semen. But semen is more than just a carrier for HIV-1. Evidence from in vitro and in vivo studies supports both inhibitory and enhancing effects. Intrinsic antiviral activity mediated by cationic antimicrobial peptides, cytotoxicity, and blockage of HIV–dendritic cell interactions are seminal plasma properties that inhibit HIV-1 infection. On the contrary, neutralization of vaginal acidic pH, enhanced virus–target cell attachment by seminal amyloid fibrils, opsonization by complement fragments, and electrostatic interactions are factors that facilitate HIV-1 infection. The end result, i.e., inhibition or enhancement of HIV mucosal infection, in vivo, likely depends on the summation of all these biological effects. More research is needed, especially in animal models, to dissect the role of these factors and establish their relevance in HIV-1 transmission.


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Behind-the-scenes look at TV opening credit creation
Column | Oct 10, 2014 | 8:41 AM
Behind-the-scenes look at TV opening credit creation
Column | Oct 10, 2014 | 8:41 AM
Emmys: Quirky Is the New Black
Story | Jul 30, 2014 | 12:30 PM
In much the same way that dramas like AMC's "Breaking Bad" and HBO's "The Sopranos" made flawed antiheroes easy to root for, a new wave of comedy is putting the oddball front and center. Instead of Jerry Seinfeld playing the straight man to his quirky...
Ensemble TV Actors Shine in Smaller Roles
Story | Jun 10, 2014 | 12:30 PM
Let's be honest: While it's great to win an Emmy -- any Emmy -- almost every actor would prefer a leading role to a supporting one. That said, some of today's top movie stars got their start in a supporting TV role. Woody Harrelson first came to fame...
'Jennifer Falls' on TV Land plays it safe
Column | Jun 4, 2014 | 6:00 AM


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Usc Shoah Foundation Institute For Visual History And Education News
High PrioritiesOctober 30, 2012
Johnny Strange's life is anything but ordinary. At the tender age of 17, when most high school juniors are busy preparing for their SATs, Strange was embarking on a journey to climb the world's highest…
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The University of Haifa has become the first university in Israel with access to the USC Shoah Foundation Institute for Visual History and Education's archive, a searchable repository of nearly 52,000 video interviews…
Disney's Robert A. Iger Receives Ambassador for Humanity AwardJune 14, 2012
Steven Spielberg, founder of the USC Shoah Foundation Institute for Visual History and Education, housed in USC Dornsife, presented Chairman and CEO of The Walt Disney Company Robert A. Iger with the institute's highest…
Strength Through AdversityMarch 28, 2012
USC students, faculty and the public listened to Rose Mapendo's harrowing story of living in a death camp with her children in the Democratic Republic of the Congo. Although Mapendo's husband was executed in the…
USC Shoah Foundation Institute Launches IWitnessJanuary 30, 2012
More than 350 New York high school students had watched parts of Holocaust survivor Roman Kent's video testimony about living in Lodz, Poland, when the Germans invaded during World War II. The young Roman, his parents,…
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July 15 (Bloomberg) -- Howard Davidowitz, chairman at Davidowitz & Associates, discusses how the financial troubles of Wal-Mart's target customers are hurting the retailer, how its foray into the lending business may help it to grow and the health of the retail consumer in America. He speaks on Bloomberg Television's "Bloomberg Surveillance."
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Every weekend, Fall Out Boy's Pete Wentz shares his favorite SiriusXM Hits 1 songs and exclusive music from his personal stash! Follow Pete and let him know what you think is a hit or a miss at twitter.com/petewentz and facebook.com/petewentz. All summer long, … Continued
What's On
Pete Wentz's Hits & Misses (7/26/14)
Hear Theory of a Deadman perform LIVE on Octane
Airs Wednesday 7/30 at 8 pm ET on Octane, Ch. 37 Rebroadcasts Thursday 7/31 at 12 am and 5 pm ET, Friday 8/1 at 12 pm ET, Saturday 8/2 at 10 pm ET, Sunday 8/3 at 3 pm ET In … Continued
This Week On Demand: Tom Petty + The Best of EW Radio at Comic-Con 2014
In celebration of the return of Tom Petty Radio, the Rock & Roll Hall of Fame legend sits down with an audience of select SiriusXM subscribers for another edition of SiriusXM's Town Hall series. Plus, Entertainment Weekly Radio will recap 2014 … Continued
SiriusXM is free fallin' into Tom Petty Radio on Classic Rewind
Tom Petty Radio debuts July 25 at 6 pm ET – August 4 at 3 am ET on Classic Rewind, Ch. 25 and Tom Petty Radio and the Limited Engagements 2 channel on SXM Internet Radio. In honor of Tom's … Continued
They look so perfect: recapping 5 Seconds of Summer's RIDICULOUS day at SiriusXM Hits 1
It's been a CRAZY few months for Australian four-piece 5 Seconds of Summer, and that's putting it lightly. The band, which is comprised of Luke Hemmings, Michael Clifford, Calum Hood, and Ashton Irwin, has JUST released their debut, self-titled album, … Continued
Hear every show from Pearl Jam's 2014 European Tour on Pearl Jam Radio
If you love Pearl Jam, then you might already know that the legendary band just wrapped up the European leg of their 2014 tour. But you know what they say about Europe and America: They're very far apart. Like, what … Continued
YouTube's top tunes: Get the latest with Jenna Marbles (7/21/14)
This week's YouTube 15 features two prominent Songs of Summer: Ariana Grande's Problem and Fancy, by Iggy Azalea and Charli XCX. And, as you may well know, the YouTube 15 on SiriusXM Hits 1 is also about promoting less well-known … Continued
Pete Wentz's Hits & Misses (7/19/14)
Every weekend, Fall Out Boy's Pete Wentz shares his favorite SiriusXM Hits 1 songs and exclusive music from his personal stash! Follow Pete and let him know what you think is a hit or a miss at twitter.com/petewentz and facebook.com/petewentz. All summer long, … Continued
5 Seconds of Summer to host the Weekend Countdown, Hit-Bound + Track-by-Track of their debut album on Hits 1!
5 Seconds of Summer is all over the place lately! And by "place," we mean SiriusXM Hits 1. That's right, there's a ton of things happening in the 5SOS/Hits 1 camp: Come Friday 7/17 at 10 pm ET, Hits 1 … Continued
Wendover –> Grand Junction: The Highway playlist from Al Skop
"Country music is all about the lyrics," says Al Skop, lead programmer and DJ for SiriusXM The Highway. "Not only does it have to have a good tempo and beat that makes you wanna open the sunroof and GO, but … Continued


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Beach Beer by Loretta Cassiano
#19 of 48
beach greeting cards, beer greeting cards, puerto rico greeting cards, medalla beer greeting cards, light beer greeting cards, sand greeting cards, sun greeting cards, ocean greeting cards, wind greeting cards, gate greeting cards, fence greeting cards
Cold Beer On a Hot Beach
When you are younger, the camera is like a friend and you can go places and feel like you're with someone, like you have a companion. ~ Annie Leibovitz ~ I love my camera and I love the results I get with it. I like to call myself a Visual Artist. My photographs are meaningful to me and hold a special place in my heart. Each and everyone tells a story and all my captures are artwork that can live on in time. I hope you enjoy and thank you for looking and hopefully, thank you for your purchase. Loretta (Dizzycat911)
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Private bus owners of Purushottampur in Ganjam district decided to ply their buses from Friday following a meeting to settle the tussle that had led them to stop movement of their vehicles. Since past five days they were not plying their buses due to a tussle over routes. The meeting held on Thursday was chaired by Chatrapur SDPO Santanu Padhi was attended by the two warring bus owners associations of Purushottampur and dignitaries of the town. A five-member committee was formed to settle the dispute.
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The gardens viewed from the woods at Harlow Carr RHS garden near Harrogate, Yorkshire.
A very pretty garden, nice work Colin …
Thanks a lot Tom, appreciated.
– Colin Metcalf
beautiful work!
Thanks a lot John.
– Colin Metcalf
What a stuning capture of this beautiful Garden Colin well done love it lee
Cheers Lee, appreciated.
– Colin Metcalf
I do love all those shades of green Colin – well captured !
Thanks mate, appreciated.
– Colin Metcalf
Beautiful garden Colin and I love those clouds !
Thank you very much Antoinette, it was a gorgeous day.
– Colin Metcalf
Lush and beautiful garden – no shortage of rain, obviously!
Thanks Bette, two days of almost solid rain before this was taken.
– Colin Metcalf
Oooh, so lush and beautiful, dear Colin!!! :)))
Cheers Laurie, much obliged.
– Colin Metcalf
Stunning work!!
Once again thank you Gena.
– Colin Metcalf
this ismagnific work !
You are so kind, thank you dear friend.
– Colin Metcalf
very beautifully captured Colin!
Many thanks LeRoy, greatly appreciated.
– Colin Metcalf


text:
Need a New Accountant? Get a Quote Online
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Vanessa Hudgens14 December 1988, Salinas, California, USA
25 years
Zac Efron, Josh Hutcherson
Vanessa Hudgens used to work as a model. Loves scary movies as much as she loves the Porsche Carrera GT. Best friends with Ashley Tisdale and Miley Cyrus. Loves Alicia Keys and Celine Dion. Her favorite singers are Alicia Keys and Céline Dion and her favorite subject in school is science. Vanessa recorded her debut album, "V", in just under two months and it has officially sold over 500,000 copies and is certified gold. When she reaches college she wants to be a business major. She met her former boyfriend Zac Efron on the set of High School Musical.
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Fashion Battle!
This Week's Best Dressed Celebrities!
Monday 11:37 AM, 22/03/2010
Wow what a fabulous fashion week! We were so impressed with young stars like Kristen Stewart, Dakota Fanning and Vanessa Hudgens and their gorgeous styles! The biggest surprise of the week had to be Jennifer Anniston, letting go of her usual simple black dress on the red carpet and wearing a Angelina Jolie-like draped dress. We think it worked for her very well! See who is at the top of the list below!
Who is your best dressed?
Tags: Best dressed celebrities / Twilight / Pucci / Sunglasses / Handbag / Red Carpet / Street Style / Lanvin
Red Carpet
All The Hottest Stars in Vegas!
Friday 1:09 PM, 19/03/2010
Wow, this is the most celeb-packed red carpet we have seen since ummm...maybe even before the Oscars! Some of TV and movie's brightest stars are in Las Vegas at the moment for the Warner Brothers Showest Showbiz convention. Check out award winners like Katherine Heigl, the Sex and the City ladies plus Vanessa Hudgens in her sexy outfit! Enjoy!
Tags: Katherine Heigl / Sex and the City / Celebrity party / Sexy celebrity / Red Carpet
Fashion Battle!
This Week's Best Dressed Celebrities!
Sunday 6:51 PM, 14/03/2010
This week's list is full of young and upcoming fashionistas who got it right like Dakota Fanning and Carey Mulligan! There were one or two old favourites too like Sarah Jessica Parker and Ell Macpherson who just never seem to put a fashion foot wrong! Take a look and see what we mean!
who is your favourite?
Tags: Elle Macpherson / Louis Vuitton / Oscars / Fashion pictures / Best dressed celebrities / Red Carpet
Vanessa Hudgens Follows Her Own Style!
Sunday 10:12 AM, 14/03/2010
While some celebrities are slaves to new trends others have managed to find their own style! Vanessa Hudgens loves her boho chic look and we have to say that we do too! We spotted the Hollywood starlet in Santa Monica yesterday at a photo shoot wearing a floppy hat, a romantic white blouse and skinny jeans. Simple and cute!
Tags: Vanessa Hudgens / Paparazzi / Hollywood celebrity / Hot
The Oscars Partying Never Stops!
Monday 9:33 PM, 08/03/2010
Just because you didn't attend the main even at the Academy Awards ceremony, doesn't mean you can't have fun at the after-parties! Just ask hot couples like Katy Perry and Russell Brand and Vanessa Hudgens and Zac Efron! Zac was the only one lucky enough to get an invite to the Oscars ceremony of those 4, but they were all at the legendary Vanity Fair after party, rubbing star shoulders with celebs like Kate Bosworth, Jessica Simpson and Taylor Lautner! Lucky them!
Tags: Oscars / Hot / Celebrity party
Style Tip: Everyone's Wearing Romantic, Curly Updos!
Monday 5:14 PM, 01/02/2010
Country singing sensation Taylor Swift has made pretty and romantic curly hairdos her signature red carpet look, but she is not the only star who loves this style! Taylor's BFF Miley Cyrus has been seen wearing similar curly looks and so have fashionistas like Jessica Alba, Scarlett Johansson and Vanessa Hudgens. Take a look who else loves this style!
Who wears it best?
Tags: Taylor Swift / Twilight / Hollywood celebrity / Hairstyles / Cool celebrities / Red Carpet


text: Jake O'Donnell 12:19 pm, May 30th, 2014 Someone in the background can be heard saying, "This is why I don't go to Planet Fitness." Really? Does this kind of stuff happen there often? Damn. Related posts: UGHHHHHH: This Gym Grunting Prank Is Fantastic You Should Feel Bad About Yourself Because This One-Armed Woman Can Weightlift (VIDEO) Merciless Man Destroys Little Kid In Pickup Basketball Game, Turns Down For Nothing (VIDEO) Goals From Germany-Portugal: Thomas Müller Has A Hat Trick; Hummels Heads In Another tags: Gym Previous Post 2014 World Cup Preview Group G: Group Of Death For U.S. And Roosters Soccer Uncategorized May 30, 2014 Next Post Mike Cardano's Daily Fantasy Baseball Picks For May 30th Fantasy MLB Video May 30, 2014


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Japan's government is currently considering raising patients'out-of-pocket payments for health care,…
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- Developer: United Front Games
- Publisher: Square Enix
- Genre: Action Adventure Modern
- Release Date: Oct 23, 2012
- ESRB: Mature
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Got some comments, feedback, and questions?Send them over to [email protected]. We'd love to hear from you!
pano kung halimbawa you take the pill in the evening but not in the same time? but religious mo naman syang tinitake? yung nga lang may time difference. will you get preggy ba???
to anonymously hot: you do? how long ka na ba on pill? ako kasi i started palang with my first pack and i had unprotected sex after 12 days of taking it. it says kasi na you are safe na one week after taking the pill? is there a possibility ba na mabubuntis ka? should i start worrying na ba? :|
new user po ako kasi ng lady pill actually pangatlong araw ko pa lang po.. Tama po bang nagsimula akong uminom sa unang huling araw ng regla ko? Natapos yung regla ko nung 27 at ngsimula akong uminom nung 28.. Tama o okay lang po ba? Salamat
Thanks mga sis! Im on my brown tablets na. But wala parin period ko.:-( normal ba na delayed ka while taking the pills?
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Cancer biology & therapy (Canc Biol Ther )
Cancer, the second leading cause of death, is a heterogenous group of over 100 diseases. Cancer is characterized by disordered and deregulated cellular and stromal proliferation accompanied by reduced cell death with the ability to survive under stresses of nutrient and growth factor deprivation, hypoxia, and loss of cell-to-cell contacts. At the molecular level, cancer is a genetic disease that develops due to the accumulation of mutations over time in somatic cells. The phenotype includes genomic instability and chromosomal aneuploidy that allows for acceleration of genetic change. Malignant transformation and tumor progression of any cell requires immortalization, loss of checkpoint control, deregulation of growth, and survival. A tremendous amount has been learned about the numerous cellular and molecular genetic changes and the host-tumor interactions that accompany tumor development and progression. It is the goal of the field of Molecular Oncology to use this knowledge to understand cancer pathogenesis and drug action, as well as to develop more effective diagnostic and therapeutic strategies for cancer. This includes preventative strategies as well as approaches to treat metastases. With the availability of the human genome sequence and genomic and proteomic approaches, a wealth of tools and resources are generating even more information. The challenge will be to make biological sense out of the information, to develop appropriate models and hypotheses and to translate information for the clinicians and the benefit of their patients. Cancer Biology & Therapy aims to publish original research on the molecular basis of cancer, including articles with translational relevance to diagnosis or therapy. We will include timely reviews covering the broad scope of the journal. The journal will also publish op-ed pieces and meeting reports of interest. The goal is to foster communication and rapid exchange of information through timely publication of important results using traditional as well as electronic formats. The journal and the outstanding Editorial Board will strive to maintain the highest standards for excellence in all activities to generate a valuable resource.
- Impact factor3.29
- 5-year impact3.33
- Cited half-life4.70
- Immediacy index0.39
- Eigenfactor0.02
- Article influence1.00
- WebsiteCancer Biology and Therapy website
- Other titlesCancer biology & therapy (Online), Cancer biology & therapy, Cancer biology and therapy
- Material typeDocument, Periodical, Internet resource
- Document typeInternet Resource, Computer File, Journal / Magazine / Newspaper
Publisher details
- Pre-print
- Author cannot archive a pre-print version
- Post-print
- Author can archive a post-print version
- Conditions
- Authors final version only
- On Institutional Repositories
- Must link to publisher version
- Published source must be acknowledged
- Landes Bioscience will deposit in PubMed Central or Europe PMC within 6-12 months of publication, depending on funding agency policy
- Embargoes on funding agency requirements, can be removed by payment of Open Access fee
- Publisher's version/PDF cannot be used
- Classification blue
Publications in this journal
Article: mTOR inhibitors blunt the p53 response to nucleolar stress by regulating RPL11 and MDM2 levels.[Show abstract] [Hide abstract]
ABSTRACT: Mechanistic target of rapamycin (mTOR) is a master regulator of cell growth through its ability to stimulate ribosome biogenesis and mRNA translation. In contrast, the p53 tumor suppressor negatively controls cell growth and is activated by a wide range of insults to the cell. The mTOR and p53 signaling pathways are connected by a number of different mechanisms. Chemotherapeutics that inhibit ribosome biogenesis often induce nucleolar stress and activation of p53. Here we have investigated how the p53 response to nucleolar stress is affected by simultaneous mTOR inhibition in osteosarcoma and glioma cell lines. We found that inhibitors of the mTOR pathway including rapamycin, wortmannin, and caffeine blunted the p53 response to nucleolar stress induced by actinomycin D. Synthetic inhibitors of mTOR (temsirolimus, LY294.002 and PP242) also impaired actinomycin D triggered p53 stabilization and induction of p21. Ribosomal protein L11 (RPL11) is known to be required for p53 protein stabilization following nucleolar stress. Treatment of cells with mTOR inhibitors may lead to reduced synthesis of RPL11 and thereby destabilize p53. We found that rapamycin mimicked the effect of RPL11 depletion in terms of blunting the p53 response to nucleolar stress. However, the extent to which the levels of p53 and RPL11 were reduced by rapamycin varied between cell lines. Additional mechanisms whereby rapamycin blunts the p53 response to nucleolar stress are likely to be involved. Indeed, rapamycin increased the levels of endogenous MDM2 despite inhibition of its phosphorylation at Ser-166. Our findings may have implications for the design of combinatorial cancer treatments with mTOR pathway inhibitors.Cancer biology & therapy 08/2014; 15(11).
Article: A novel small-molecule compound diaporine A inhibits non-small cell lung cancer growth by regulating miR-99a/mTOR signaling.[Show abstract] [Hide abstract]
ABSTRACT: MicroRNAs (miRNAs) dysregulation is critically involved in lung cancer. Regulating miRNAs by natural agents may be a new strategy for cancer treatment. We previously found that a novel small-molecule compound diaporine A (D261), a natural product of endophytic fungus 3lp-10, had potential anti-cancer activites. In the present study, the inhibitory effect of D261 on non-small cell lung cancer (NSCLC) growth and its possible mechanisms involving miRNA regulation were investigated. By cell viability assay, cell proliferation analysis and clonal growth assay, we proved that D261 effectively inhibited the proliferation of NSCLC cells (NCI-H460 and A549) in vitro. Administration of D261 (5 mg/kg) to NCI-H460 xenografts bearing mice also inhibited tumor growth and decreased the expression of cell proliferation regulator, midkine. Moreover, D261 induced cell cycle arrest with a reduced expression of various G 1/S transition-related molecules including cyclin D1, cyclin E1, CDK4 and CDK2, but without influencing apoptosis in NSCLC cells. Intriguingly, D261 modified expressions of some miRNAs and especially upregulated miR-99a, whose direct target was mammalian target of rapamycin (mTOR). Furthermore, overexpression of miR-99a antagonized the anti-tumor actions of D261 including the suppression of mTOR pathway activation, cell cycle-related proteins and cell growth. In addition, blocking of miR-99a expression by transfection of miR-99a inhibitors before D261 treatment counteracted the anti-tumor effects of D261. These data suggest that miR-99a/mTOR pathway was involved in D261-induced tumor suppression in NSCLC cells. D261 might be a potent anti-cancer agent by upregulating miR-99a expression.Cancer biology & therapy 07/2014; 15(10).
Article: Ipilimumab: A potential immunologic agent in the treatment of metastatic castration-resistant prostate cancer.[Show abstract] [Hide abstract]
ABSTRACT: Immunotherapy has garnered an important place in the therapeutic landscape of treatment in prostate cancer since approval of sipuleucel-T. Ipilimumab is a checkpoint inhibitor that is currently approved for the treatment of advanced melanoma. In the May issue of Lancet Oncology, Kwon and colleagues report the phase III trial using ipilimumab in a post-docetaxel metastatic castration-resistant prostate cancer population. While the primary endpoint of overall survival was not met, several lessons are learned from the analysis of this trial. Perhaps better refinement of a more favorable group of patients who may potentially benefit from an immunologic treatment should be advocated.Cancer biology & therapy 07/2014; 15(10).
Article: Production of IL-1β by bone marrow-derived macrophages in response to chemotherapeutic drugs: Synergistic effects of doxorubicin and vincristine.[Show abstract] [Hide abstract]
ABSTRACT: Cytotoxic chemotherapeutic drugs, especially when used in combination, are widely employed to treat a variety of cancers in patients but often lead to serious symptoms that negatively affect physical functioning and quality of life. There is compelling evidence that implicates cytotoxic chemotherapy-induced inflammation in the etiology of these symptoms. Because IL-1β plays a central role as an initiator cytokine in immune responses, we compared doxorubicin, a drug known to induce IL-1β production, with ten other commonly prescribed chemotherapeutic drugs in their ability to lead to processing and secretion of IL-1β by primary mouse macrophages. Seven of them (melphalan, cisplatin, vincristine, etoposide, paclitaxel, methotrexate, and cytarabine) caused the production of IL-1β in cells pretreated with lipopolysaccharide. When delivered in combination with doxorubicin, one of the drugs, vincristine, was also capable of synergistically activating the NLRP3-dependent inflammasome and increasing expression of IL-1β, IL-6 and CXCL1. The absence of TNF-α and IL-1 signaling caused a partial reduction in the production of mature IL-1β. Three small-molecule inhibitors known to suppress activity of kinases situated upstream of mitogen-activated kinases (MAPKs) inhibited the expression of IL-1β, IL-6 and CXCL1 when doxorubicin and vincristine were used singly or together. Kinase inhibitors may be useful in reducing inflammation in patients receiving chemotherapy.Cancer biology & therapy 07/2014; 15(10).
- [Show abstract] [Hide abstract]
ABSTRACT: Chemoresistance is a major therapeutic challenge to overcome in NSCLC, in order to improve the current survival rates of<15% at 5 years. We and others have shown increased PI3K signaling in NSCLC to be associated with a more aggressive disease, and a poorer prognosis. In this study, targeted inhibition of three strategic points of the PI3K-NFκB axis was performed with the aim of exploiting vulnerabilities in cisplatin resistant NSCLC cells. Cisplatin resistant cell lines were previously generated through prolonged exposure to the drug. Expression of PI3K and NFκB pathway related genes were compared between cisplatin resistant cells and their matched parent cells using a gene expression array, qRT-PCR, DNA sequencing, western blot and immunofluorescence. Targeted inhibition was performed using GDC-0980, a dual PI3K-mTOR inhibitor currently in Phase II clinical trials in NSCLC, and DHMEQ, an inhibitor of NFκB translocation which has been used extensively both in vitro and in vivo. Effects of the two inhibitors were assessed by BrdU proliferation assay and multiparameter viability assay. NFKBIA was shown to be 12-fold overexpressed in cisplatin resistant cells, with no mutations present in exons 3, 4, or 5 of the gene. Corresponding overexpression of IκBα was also observed. Treatment with DHMEQ (but not GDC-0980) led to significantly enhanced effects on viability and proliferation in cisplatin resistant cells compared with parent cells. We conclude that NFκB inhibition represents a more promising strategy than PI3K-mTOR inhibition for treatment in the chemoresistance setting in NSCLC.Cancer biology & therapy 07/2014; 15(10).
Article: Dissecting the roles of E1A and E1B in adenoviral replication and RCAd-enhanced RDAd transduction efficacy on tumor cells.[Show abstract] [Hide abstract]
ABSTRACT: Oncolytic viruses have recently received widespread attention for their potential in innovative cancer therapy. Many telomerase promoter-regulated oncolytic adenoviral vectors retain E1A and E1B. However, the functions of E1A and E1B proteins in the oncolytic role of replication-competent adenovirus (RCAd) and RCAd enhanced transduction of replication defective adenoviruses (RDAd) have not been addressed well. In this study, we constructed viruses expressing E1A alone, E1A plus E1B-19 kDa, and E1A plus E1B-19 kDa/55 kDa. We then tested their roles in oncolysis and replication of RCAd as well as their roles in RCAd enhanced transfection rate and transgene expression of RDAd in various cancer cells in vitro and in xenografted human NCI-H460 tumors in nude mice. We demonstrated that RCAds expressing E1A alone and plus E1B-19 kDa exhibited an obvious ability in replication and oncolytic effects as well as enhanced RDAd replication and transgene expression, with the former showed more effective oncolysis, while the latter exhibited superior viral replication and transgene promotion activity. However, RCAd expressing both E1A and E1B-19kDa/55kDa was clearly worst in all these abilities. The effects of E1A and E1B observed through using RCAd were further validated by using plasmids expressing E1A alone, E1A plus E1B-19kDa, and E1A plus E1B-19kDa/55kDa proteins. Our study provided evidence that E1A was essential for inducing replication and oncolytic effects of RCAd as well as RCAd enhanced RDAd transduction, and expression of E1B-19kDa other than E1B-55kDa could promote these effects. E1B-55kDa is not necessary for the oncolytic effects of adenoviruses and somehow inhibits RCAd-mediated RDAd replication and transgene expression.Cancer biology & therapy 07/2014; 15(10).
Article: HIF1α-independent hypoxia-induced rapid PTK6 stabilization is associated with increased motility and invasion.[Show abstract] [Hide abstract]
ABSTRACT: PTK6/Brk is a non-receptor tyrosine kinase overexpressed in cancer. Here we demonstrate that cytosolic PTK6 is rapidly and robustly induced in response to hypoxic conditions in a HIF-1 independent manner. Furthermore, a proportion of hypoxic PTK6 subsequently re-localized to the cell membrane. We observed that the rapid stabilization of PTK6 is associated with a decrease in PTK6 ubiquitylation and we have identified c-Cbl as a putative PTK6 E3 ligase in normoxia. The consequences of hypoxia-induced PTK6 stabilization and subcellular re-localization to the plasma membrane include increased cell motility and invasion, suggesting PTK6 targeting as a therapeutic approach to reduce hypoxia-regulated metastatic potential. This could have particular significance for breast cancer patients with triple negative disease.Cancer biology & therapy 07/2014; 15(10).
Article: MiR-20a inhibits cutaneous squamous cell carcinoma metastasis and proliferation by directly targeting LIMK1.[Show abstract] [Hide abstract]
ABSTRACT: Background MicroRNA-20a (miR-20a) plays a key role in tumorigenesis and progression. But its function is reverse in different kinds of malignant tumor, and its role and mechanism in cutaneous squamous cell carcinoma (CSCC) remains unclear. Object To determine the miR-20a's roles in CSCC and confirm whether LIMK1 is a direct target gene of miR-20a. Methods First miR-20a and LIMK1 expression levels were detected in six pairs of CSCC tissues and corresponding normal skin by qRT-PCR. Then MTT assays and colony formation assays were performed to evaluate the impact of miR-20a on cell proliferation. In addition, scratch migration assays and transwell invasion assays were performed to check miR-20a's effect on cell metastasis. Since LIMK1 (LIM kinase-1) was predicted as a target gene of miR-20a, the changes of LIMK1 protein and mRNA were measured by western blot and qRT-RCR methods after miR-20a overexpression. Moreover the dual reporter gene assay was performed to confirm whether LIMK1 is a direct target gene of miR-20a. Finally LIMK1 mRNA and miR-20a in other 30 cases of CSCC pathological specimens were determined and a correlation analysis was evaluated. Results The miR-20a significantly low-expressed in CSCC tissues compared with that in matched normal tissues while LIMK1 has a relative higher expression. MiR-20a inhibited A431 and SCL-1 proliferation and metastasis. Both of LIMK1 protein and mRNA levels were downregulated after miR-20a overexpression. The dual reporter gene assays revealed that LIMK1 is a direct target gene of miR-20a. Furthermore, qRT-PCR results of LIMK1 mRNA and miR-20a in 30 cases of CSCC pathological specimens showed miR-20a is inversely correlated with LIMK1 expression. Conclusion Our study demonstrated that miR-20a is involved in the tumor inhibition of CSCC by directly targeting LIMK1 gene. This finding provides potential novel strategies for therapeutic interventions of CSCC.Cancer biology & therapy 07/2014; 15(10).
Article: Arsenite promotes intestinal tumor cell proliferation and invasion by stimulating epithelial-to-mesenchymal transition.[Show abstract] [Hide abstract]
ABSTRACT: Arsenite (AS) is a ubiquitous environmental element that is widely present in food, soil, and water. Environmental exposure to AS represents a major global health concern, because AS is a well-established human carcinogen. We hypothesize that low concentration of AS could enhance metastasis and proliferation of transformed cancer cells by promoting EMT. To test this hypothesis, we treated human colorectal cancer cells with low concentration of AS, and then measured the multiple readouts of cell viability, proliferation, migration, and adhesion in vitro and in vivo. Collectively, our data indeed strongly support our hypothesis and shed novel light into this important pathophysiological process. These novel insights are not only of high interests to basic cancer research, but may also have direct implications in cancer prevention and treatment.Cancer biology & therapy 07/2014; 15(10).
Article: CDK4 inhibition and doxorubicin mediate breast cancer cell apoptosis through Smad3 and survivin.[Show abstract] [Hide abstract]
ABSTRACT: Cyclin D1/CDK4 activity is upregulated in up to 50% of breast cancers and CDK4 mediated phosphorylation negatively regulates the TGFβ superfamily member Smad3. We sought to determine if CDK4 inhibition and doxorubicin chemotherapy could impact Smad3-mediated cell/colony growth and apoptosis in breast cancer cells. Parental and cyclin D1 overexpressing MCF7 cells were treated with CDK4 inhibitor, doxorubicin, or combination therapy and cell proliferation, apoptosis, colony formation, and expression of apoptotic proteins were evaluated using an MTS assay, TUNEL staining, 3D Matrigel assay, and apoptosis array/immunoblotting. Study cells were also transduced with WT Smad3 or a Smad3 construct resistant to CDK4 phosphorylation (5M) and colony formation and expression of apoptotic proteins were assessed. Treatment with CDK4 inhibitor/doxorubicin combination therapy, or transduction with 5M Smad3, resulted in a similar decrease in colony formation. Treating cyclin D overexpressing breast cancer cells with combination therapy also resulted in the greatest increase in apoptosis, resulted in decreased expression of anti-apoptotic proteins survivin and XIAP, and impacted subcellular localization of pro-apoptotic Smac/DIABLO. Additionally, transduction of 5M Smad3 and doxorubicin treatment resulted in the greatest change in apoptotic protein expression. Collectively, this work showed the impact of CDK4 inhibitor-mediated, Smad3-regulated tumor suppression, which was augmented in doxorubicin-treated cyclin D overexpressing study cells.Cancer biology & therapy 07/2014; 15(10).
Article: Prostate tumor development and androgen receptor function alterations in a new mouse model with ERG overexpression and PTEN inactivation.[Show abstract] [Hide abstract]
ABSTRACT: Gene fusions involving ETS transcription factors (predominantly ERG and ETV1) and PTEN deletions are prevalent in the prostate cancer genome. This report describes a novel mouse model that overexpresses ERG and lacks PTEN with the majority of mice developing prostate tumors by six months. Biological mechanisms suggest increased/altered binding of the male hormone receptor in the genome. This model will be useful in pre-clinical evaluation of new drugs targeting these common prostate cancer genomic alterations.Cancer biology & therapy 07/2014; 15(10).
Article: Chk1-induced CCNB1 overexpression promotes cell proliferation and tumor growth in human colorectal cancer.[Show abstract] [Hide abstract]
ABSTRACT: The high morbidity and mortality of colorectal cancer pose a significant public health problem worldwide. Here we assessed the pro-cancer efficacy and mechanism of action of CCNB1 in different colorectal cancer cells. We provided evidence that CCNB1 mRNA and protein level were upregulated in a subset of human colorectal tumors, and positively correlated with Chk1 expression. Repression of Chk1 caused a significant decrease in cell proliferation and CCNB1 protein expression in colorectal cancer cells. Furthermore, downregulation of CCNB1 impaired colorectal cancer proliferation in vitro and tumor growth in vivo. Specifically, suppression of CCNB1 caused a strong G 2/M phase arrest in both HCT116 and SW480 cells, interfering with the expression of cdc25c and CDK1. Additionally, CCNB1 inhibition induced apoptotic death in certain colorectal cancer cells. Together, these results suggest that CCNB1 is activated by Chk1, exerts its oncogenic role in colorectal cancer cells, and may play a key role in the development of a novel therapeutic approach against colorectal cancer.Cancer biology & therapy 06/2014; 15(9).
Article: Expression profiling of serum microRNA-101 in HBV-associated chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma.[Show abstract] [Hide abstract]
ABSTRACT: MicroRNAs (miRNAs) represent a class of evolutionarily conserved, non-coding small RNAs (18-25 nt) that have emerged as master regulators of several biological processes. Recently, circulating miRNAs have also been reported to be promising biomarkers for various pathological conditions. In the present study, we report the comparative expression profiling of microRNA-101 (miR-101) in serum and tissue samples from chronic hepatitis B (CHB), HBV-associated liver cirrhosis (HBV-LC), and HBV-associated hepatocellular carcinoma (HBV-HCC) patients and healthy controls. The serum miR-101 levels were found to be significantly downregulated in the HBV-HCC patients compared with the HBV-LC patients (P<0.001), CHB patients (P<0.001) and healthy controls but were upregulated in the HBV-LC patients compared with the CHB patients (P<0.001) and healthy controls (P<0.001). Consistent with the serum data, the expression of miR-101 was also upregulated and downregulated in the HBV-LC and HBV-HCC tissue samples, respectively. A receiver operating characteristic (ROC) analysis of serum miR-101 yielded an area under the ROC curve (AUC) of 0.976 with 95.5% sensitivity and 90.2% specificity when differentiating between HBV-HCC and HBV-LC. Our results suggest that the serum miR-101 level can serve as a potential non-invasive biomarker to differentiate HBV-HCC from HBV-LC.Cancer biology & therapy 06/2014; 15(9).
Article: The prognostic significance of combined ERG and androgen receptor expression in patients with prostate cancer managed by androgen deprivation therapy.[Show abstract] [Hide abstract]
ABSTRACT: ERG and androgen receptor (AR) are known to function cooperatively in prostate cancer (PCa) progression. However, the prognostic value of combined ERG and AR expression and potential pathways are not well characterized. We assessed ERG and AR protein expression by immunohistochemistry in a cohort of 312 men with PCa diagnosed by transurethral resection of the prostate (TURP). Patients were divided into those with no prior treatment (designated as PCa/AdvPCa) vs. those with castrate-resistant PCa (CRPC) undergoing channel TURP to relieve obstructive symptoms. The expression status was correlated with various clinical-pathological parameters. The Swedish watchful-waiting cohort was used for validation and characterization of potential gene signatures associated with ERG and AR. Patients with combined ERG-positive/AR high expression profile demonstrated higher rates of PCa-specific mortality (PCSM) compared with patients with ERG-negative/AR low in patients with no prior treatment (n = 90, P = 0.032), but this was attenuated in the overall cohort which included the CRPC subgroup (n = 125, P = 0.096). The prognostic significance to PCSM was validated in the Swedish watchful waiting cohort in univariate (HR: 3.3; 95% CI: 1.9-5.6, P = 4.25E-5) and multivariate analysis (HR: 2; 95% CI: 0.97-4.1, P = 0.057), which included Gleason score. ERG/AR overexpression status characterized 152 genes signatures including WNT, PI3K/AKT and chemokine signaling pathways known to be deregulated in PCa. In conclusion, combined ERG/AR overexpression signifies a class of patients at highest-risk of PCSM with specific key genetic alteration likely responsible for disease progression. The prognostic value of combined ERG/AR overexpression and its associated genes should be further investigated as potential prognostic and therapeutic targets in prostate cancer progression.Cancer biology & therapy 06/2014; 15(9).
Article: An improved isoprenylcysteine carboxylmethyltransferase inhibitor induces cancer cell death and attenuates tumor growth in vivo.[Show abstract] [Hide abstract]
ABSTRACT: Inhibitors of isoprenylcysteine carboxylmethyltransferase (Icmt) are promising anti-cancer agents, as modification by Icmt is an essential component of the protein prenylation pathway for a group of proteins that includes Ras GTPases. Cysmethynil, a prototypical indole-based inhibitor of Icmt, effectively inhibits tumor cell growth. However, the physical properties of cysmethynil, such as its low aqueous solubility, make it a poor candidate for clinical development. A novel amino-derivative of cysmethynil with superior physical properties and marked improvement in efficacy, termed compound 8.12, has recently been reported. We report here that Icmt (-/-) mouse embryonic fibroblasts (MEFs) are much more resistant to compound 8.12-induced cell death than their wild-type counterparts, providing evidence that the anti-proliferative effects of this compound are mediated through an Icmt specific mechanism. Treatment of PC3 prostate and HepG2 liver cancer cells with compound 8.12 resulted in pre-lamin A accumulation and Ras delocalization from the plasma membrane, both expected outcomes from inhibition of the Icmt-catalyzed carboxylmethylation. Treatment with compound 8.12 induced cell cycle arrest, autophagy and cell death, and abolished anchorage-independent colony formation. Consistent with its greater in vitro efficacy, compound 8.12 inhibited tumor growth with greater potency than cysmethynil in a xenograft mouse model. Further, a drug combination study identified synergistic antitumor efficacy of compound 8.12 and the epithelial growth factor receptor (EGFR)-inhibitor gefitinib, possibly through enhancement of autophagy. This study establishes compound 8.12 as a pharmacological inhibitor of Icmt that is an attractive candidate for further preclinical and clinical development.Cancer biology & therapy 06/2014; 15(9).
Article: Genetically engineered Newcastle disease virus expressing interleukin-2 and TNF-related apoptosis-inducing ligand for cancer therapy.[Show abstract] [Hide abstract]
ABSTRACT: Recombinant Newcastle disease virus (rNDV) have shown oncolytic therapeutic efficacy in preclinical studies and are currently in clinical trials. In this study, we have evaluated the possibility to enhance the cancer therapeutic potential of NDV by means of inserting both interleukin-2 (IL-2) and tumor necrosis factor-related apoptosis inducing ligand (TRAIL) delivered by rNDV. We demonstrated that rNDV expressing TRAIL (rNDV-TRAIL) or both human IL-2 and TRAIL (rNDV-IL-2-TRAIL) significantly enhanced inherent anti-neoplastic of rNDV by inducing apoptosis. And we showed that apoptosis-related genes mRNA expression was increased after treated with rNDV-TRAIL or rNDV-IL-2-TRAIL compared with rNDV and rNDV-IL-2. We also demonstrated that both rNDV-IL-2 and rNDV-IL-2-TRAIL induced proliferation of the CD4(+) and CD8(+) in treated mice and elicited expression of TNF-α and IFN-γ antitumor cytokines. These mice treated with oncolytic agents exhibited significant reduction in tumor development compared with mice treated with the parental virus. In addition, experiments in both hepatocellular carcinoma and melanoma-bearing mice demonstrated that the genetically engineered rNDV-IL-2-TRAIL exhibited prolonged animals' survival compared with rNDV, rNDV-IL-2, and rNDV-TRAIL. In conclusion, the immunotherapy and oncolytic virotherapy properties of NDV can be enhanced by the introduction of IL-2 and TRAIL genes, whose products initiated a broad cascade of immunological affects and induced tumor cells apoptosis in the microenvironment of the immune system.Cancer biology & therapy 06/2014; 15(9).
Article: Sulforaphane induces apoptosis in rhabdomyosarcoma and restores TRAIL-sensitivity in the aggressive alveolar subtype leading to tumor elimination in mice.[Show abstract] [Hide abstract]
ABSTRACT: Rhadbomyosarcoma (RMS) is the most common soft-tissue sarcoma in children and is subdivided in the embryonal (ERMS) and alveolar (ARMS) subtypes, the latter being associated with the worst prognosis. We report that sulforaphane (SFN), a broccoli-derived anticancer isothiocyanate, causes dose- and time-dependent growth inhibition and apoptosis in both ERMS and ARMS cells. In ARMS, SFN induced the modulation of expression of crucial genes and proteins: mRNA and protein levels of PAX3-FKHR, MYCN, and MET decreased, while those of p21 and TRAIL-receptor DR5 (but not DR4) increased. Since DR5 expression increased specifically in ARMS, we treated ARMS cells with TRAIL, SFN, or their combination. While ARMS cells (RH30 and RH4) proved to be TRAIL-resistant, SFN restored their sensitivity to TRAIL-induced cell-growth inhibition, leading to a stronger effect in combination with TRAIL. ARMS cells transfected with siDR5 showed that SFN-induced DR5 acts as a key regulator, being directly related to the TRAIL-induced cell-growth inhibition. The in vivo anti-tumor activity of SFN and TRAIL was evaluated in a xenograft murine model of ARMS through microPET. The results showed that the systemic treatment (3 wk) of mice with SFN or TRAIL as single agents only delayed tumor evolution, while the combined treatment of SFN and TRAIL led to tumor elimination. These findings indicate that SFN triggers the apoptotic pathway in both alveolar and embryonal rhabdomyosarcomas and that combined treatment with SFN and TRAIL might be a promising therapy for the aggressive alveolar subtype.Cancer biology & therapy 06/2014; 15(9).
Article: The interplay between histone deacetylases and c-Myc in the transcriptional suppression of HPP1 in colon cancer.[Show abstract] [Hide abstract]
ABSTRACT: HPP1 (hyperplastic polyposis protein 1), a tumor suppressor gene, is downregulated by promoter hypermethylation in a number of tumor types including colon cancer. c-Myc is also known to play a role in the suppression of HPP1 expression via binding to a promoter region cognate E-box site. The contribution of histone deacetylation as an additional epigenetic mechanism and its potential interplay with c-Myc in the transcriptional regulation of HPP1 are unknown. We have shown that the treatment of the HPP1-non-expressing colon cancer cell lines, HCT116 and DLD-1 with HDAC inhibitors results in re-expression of HPP1. RNAi-mediated knockdown of c-Myc as well as of HDAC2 and HDAC3 in HCT116 and of HDAC1 and HDAC3 in DLD-1 also resulted in significant re-expression of HPP1. Co-immunoprecipitation (IP), chromatin IP (ChIP), and sequential ChIP experiments demonstrated binding of c-Myc to the HPP1 promoter with recruitment of and direct interaction with HDAC3. In summary, we have demonstrated that c-Myc contributes to the epigenetic regulation of HPP1 via the dominant recruitment of HDAC3. Our findings may lead to a greater biologic understanding for the application of targeted use of HDAC inhibitors for anti-cancer therapy.Cancer biology & therapy 06/2014; 15(9).
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.


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Stock Market & Financial Investment News
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February 22, 2013
S&P 500 is on the cusp of trend change or confirmation, levels to watch
The index has recovered from prior day lows, reversing in mean reverting fashion to the 10-day moving average. As we have discussed before, the 10-day is an important proxy for the uptrend off the lows of December and November. In both those months, the 10-day was violated on the downside but quickly reverted to become support. We may see that behavior here, or, a change in trend. The next week will be an important test or confirmation of the longer-term uptrend. At the current level of 1512.88, resistance is at the 10-day moving average, last at 1517.40. Support is at the opening price today of 1502.42. All sectors are in the green, with Materials, Energy, Technology, and the Financials the relative upside leaders. These were among the weakest sectors yesterday.
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New York
Iranian Musicians Shot Dead in Brooklyn
BY Stephen Farrell | Nov. 12, 2013 | 1:53
The Iranian indie band the Yellow Dogs fled Tehran so that they could live and play their music free of fear and repression. But in New York, two of the band's members met sudden, violent deaths.
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Vantaa - Vladislav Delay
- Band Name: Vladislav Delay
- Record Label: Raster-Noton
- Release Date: Nov 28, 2011
- Summary: Finnish artists Sasu Ripatti, who records under a variety of names, including Luomo, Uusitalo, Sistol, and Conoco, releases his first album on the Raster-Notion label under the Vladislav Delay name.
- Record Label: Raster-Noton
- Genre(s): Electronic, Club/Dance
- More Details and Credits »
Score distribution:
Positive: 4 out of 4
Mixed: 0 out of 4
Negative: 0 out of 4
Feb 1, 2012He delivers a stirring counterpoint to Quartet with an atmospheric combination of organic and digital feels that offers a stirring dual portrait of the landscape of his motherland.
Feb 1, 2012Ripatti's restraint is still his strongest suit; he's not so much leading the way as lighting it.
Feb 1, 2012It offers astonishingly rich pickings--its pillowy-soft surfaces might have all the edges filed away, but there's a stunning amount of detail packed into each of its eight tracks.
Feb 1, 2012This music is as simultaneously functional and pleasurable as Luomo's more active house tracks, only it's for an opposite function--and a more sedate set of pleasure centers.
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