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A beautiful girl who is very sarcastic.
(Somebody comes up to her in a shirt covered in feathers and glitter)
"Don't you love my shirt?"
Samara: "Sure I do, suuure."
"Don't you love my shirt?"
Samara: "Sure I do, suuure."
by stopstaringatme12345 March 02, 2010
A natural entertainer, usually multi-talented. Easily sidetracked, and although intelligent, can be very moronic at times. Sarcastic and outgoing, a Samara has many friends but is close with few. She is interested in many things and because she never divulges too far into one topic, she can develop a reputation of being superficial. Samaras love to fool around with guys and want to be free, but never alone. Excellent taste in music, incredible body. Knows no boundaries or limits and can come off as intimidating. Nobody breaks her heart, she calls the shots and knows how to play guys. Because of this she is sometimes called a slut, but names don't affect her. Samara's never stop talking and love attention. They also have a great sense of humor and are charismatic. They can win almost anyone over.
John: Yo did that girl you like ever text you back?
Matt: No dude she's such a Samara.
Matt: No dude she's such a Samara.
by whattheactual- February 21, 2011
A very beautiful girl with a unique personality. She's sweet and sour but mostly sweet. She's usually a black girl but she can be a white girl. Good, nice, and beautiful hair. She's a great artist, loves books, fantastic writer. She's the girl you want. Samara is usually mistaken for that bitch who crawls out of the well in The Ring. But Samara will always be a beautiful name for a beautiful girl.
Damn Samara looks hot today
by tamtam27 October 04, 2010
Samara (Russian: Сама́ра) (Kuybyshev (Ку́йбышев) from 1935 to 1990) is one of the largest cities in Russia.
The Russian Revolution resulted in Soviet control of Samara in 1917, but in June 1918 under the armed support of the Czechoslovak corps the town was taken by the Committee of Members of the Constituent Assembly.
by Heronimo January 24, 2009


text:
Thinking back…Mobile marketing not a tech toy, it's the height of CRM
- How Loyalty Programs Can Boost Apple Pay Usage by @jitendragupta po.st/OZdJIh via @B2Community 5 days ago
- If you are looking to put butts in seats, look for engagement apps from @getpunchh 3 weeks ago
- RT @nhglass: Wow. "70% of restaurant operators said they planned to invest in [an app] in 2014." Come and get it! @Olo http://t.co/5iS7Tg7z… 3 weeks ago
- 9 CRM Solutions for Small Businesses shar.es/1mBDQs via @BNDarticles 1 month ago
- RT @JoshuaRM: #4 for lotz4me - fastcasual.com/articles/schlo… - @getpunchh is the only company with 2 top 10 restaurant apps - #proud RT @JitendraG… 1 month ago

subset: commoncrawl


text:
Martha's Sewing Room
HANDLOOMSSeries Website »
Episode Guide »
Creating handlooms with decorative machine stitches and a fold-over case for small accessories.
Vermont transplant and Guster frontman Ryan Miller seeks out far-fetched friends across the state! Available exclusively online.
A second HD broadcast service offering alternative programming and more content from local and regional producers.


text:
Semper Fi Do Or Die
Item Description
Follow a group of young men as they go through Marine Corps boot camp in 1962, at Parris Island, and then to their duty stations and Vietnam. If you want to know what the Corps was really like in the 1960's and those that served during this tumultuous time in history this is the book for you!
Product Details
- Author: Keith G. Laufenberg
- Publication Date: 2011-11-23
- Publisher: Royal Crown Royal Publ., LLC
- Product Group: eBooks
- Manufacturer: Royal Crown Royal Publ., LLC
- Binding: Kindle Edition, 340 pages
Buying Options
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Lassa hemorrhagic fever (LHF) is a neglected tropical disease with significant impact on the health care system, society, and economy of Western and Central African nations where it is endemic. With a high rate of infection that may lead to morbidity and mortality, understanding how the virus interacts with the host's immune system is of great importance for generating vaccines and therapeutics. Previous work by our group identified a soluble isoform of the Lassa virus (LASV) GP1 (sGP1) in vitro resulting from the expression of the glycoprotein complex (GPC) gene [1,2]. Though no work has directly been done to demonstrate the function of this soluble isoform in arenaviral infections, evidence points to immunomodulatory effects against the host's immune system mediated by a secreted glycoprotein component in filoviruses, another class of hemorrhagic fever-causing viruses. A significant fraction of shed glycoprotein isoforms during viral infection and biogenesis may attenuate the host's inflammatory response, thereby enhancing viral replication and tissue damage. Such shed glycoprotein mediated effects were previously reported for Ebola virus (EBOV), a filovirus that also causes hemorrhagic fever with nearly 90% fatality rates [3-5]. The identification of an analogous phenomenon in vivo could establish a new correlate of LHF infection leading to the development of sensitive diagnostics targeting the earliest molecular events of the disease. Additionally, the reversal of potentially untoward immunomodulatory functions mediated by sGP1 could potentiate the development of novel therapeutic intervention. To this end, we investigated the presence of sGP1 in the serum of suspected LASV patients admitted to the Kenema Government Hospital (KGH) Lassa Fever Ward (LFW), in Kenema, Sierra Leone that tested positive for viral antigen or displayed classical signs of Lassa fever.
It is reasonable to expect that a narrow window exists for detection of sGP1 as the sole protein shed during early arenaviral biogenesis. This phenomenon was clearly distinguishable from virion-associated GP1 only prior to the emergence of de novo viral particles. Despite this restricted time frame, in 2/46 suspected cases in two studies performed in late 2009 and early 2010, soluble glycoprotein component shedding was identified. Differential detection of viral antigens GP1, GP2, and NP by western blot yielded five different scenarios: whole LASV virions (GP1, GP2, NP; i.e. active viremia), different combinations of these three proteins, sGP1 only, NP only, and absence of all three proteins. Four additional samples showed inconclusive evidence for sGP1 shedding due to lack of detection of GP2 and NP by western blot; however, a sensitive LASV NP antigen capture ELISA generated marginally positive signals
During a narrow window following active infection with LASV, soluble GP1 can be detected in patient sera. This phenomenon parallels other VHF infection profiles, with the actual role of a soluble viral glycoprotein component in vivo remaining largely speculative. The expenditure of energy and cellular resources toward secretion of a critical protein during viral biogenesis without apparent specific function requires further investigation. Future studies will be aimed at systematically identifying the role of LASV sGP1 in the infection process and outcome in vitro and in vivo.
Lassa virus, a member of the Arenaviridae family, is the etiologic agent of Lassa fever, which is an acute and often fatal illness endemic to West Africa. There are an estimated 300,000 - 500,000 cases of Lassa fever each year, with a mortality rate of 15% - 20% for hospitalized patients and as high as 50% during epidemics [9,10]. Presently, there is no licensed vaccine or immunotherapy available for preventing or treating this disease. Although the antiviral drug ribavirin is somewhat beneficial, it must be administered at an early stage of infection to successfully alter disease outcome, thereby limiting its utility [11]. Furthermore, there is no commercially available Lassa fever diagnostic assay, thereby inhibiting early detection and rapid implementation of existing treatment regimens (e.g. ribavirin administration). The lack of adequate countermeasures and means of detection, coupled with the severity of disease, contributed to the classification of LASV as a National Institutes of Allergy and Infectious Diseases (NIAID) Category A pathogen and biosafety level-4 (BSL-4) agent. Presently, the Hemorrhagic Fever Virus Diagnostics Consortium is developing and implementing highly sensitive and specific next generation recombinant diagnostic assays that will be easily deployed and analyzed in remote locations of endemic areas with few laboratory resources.
The LASV genome is comprised of two ambisense, single-stranded RNA molecules designated small (S) and large (L) [12]. Two genes on the S segment encode the nucleoprotein (NP) and a precursor glycoprotein complex (GPC). The L segment encodes the viral polymerase (L protein) and RING finger Z matrix protein. The precursor GPC forms the membrane-bound GP1 and GP2 subunits via post-translational cleavage by the protease SKI-1/S1P [13]. GP1 serves a putative role in receptor binding, while the structure of GP2 is consistent with viral transmembrane fusion proteins [14]. NP binds and protects the viral RNA in the virion [15-17]. The Z matrix protein associates with GP2 and NP during viral biogenesis, but alone is sufficient to mediate formation and release of viral particles from infected/transfected cells [18-21].
Differential detection of LASV antigens in infected human patient sera
A total of 19 and 27 patient sera were analyzed in the first and second studies, respectively. A panel of serum samples from approximately 100 volunteer blood donors from the Northwestern district of Bombali, Sierra Leone, designated BOM series, were analyzed for LASV-specific antigen and IgG and IgM antibodies. These samples were collected from individuals whose reported medical histories did not contain indication of previous infection with Lassa virus. A significant percentage of BOM samples tested positive for IgM and IgG antibodies against LASV proteins in a recombinant ELISA, but all 100 were negative for virus antigen in a capture assay using an NP-specific detection platform (data not shown). Additionally, a panel of archived and freshly collected human sera from patients that had been admitted to the KGH from January 2008 - April 2010 as suspected Lassa fever cases were chosen for these studies. All archived samples containing sufficient quantities of serum (~ 20 - 25 μL) with a diagnosis of "antigen positive" (Ag+) determined by a traditional Ag-capture assay (Trad Ag) developed by the United States Army Medical Research Institute of Infectious Diseases (USAMRIID), a recombinant Ag-capture assay for LASV NP developed by the Hemorrhagic Fever Virus Diagnostics Consortium, or from patients presenting with classical clinical symptoms of Lassa fever were used in this study. In addition, a subpanel of samples that were Ag-negative but IgM and/or IgG positive, either by traditional LASV antibody platform (USAMRIID) [Trad IgG, Trad IgM] or recombinant protein-based (Hemorrhagic Fever Virus Diagnostics Consortium) [r IgG, r IgM] ELISA, were analyzed as controls. A profile of LASV antigens detected in serum samples analyzed in these studies is displayed in figure 1. All three antigens, NP, GP1, and GP2, were detected at high levels in only three patient sera (figure 1, G692-1, G762-1, G765-1). The former two patients succumbed while the outcome of G765-1 is unknown. In six independent patient sera, GP1 antigen was the only antigen detected (figure 1, G610-3, G676-A, G583-1, G755-1, G337-1, G079-3), albeit at relatively low levels compared to the triple Ag-positive samples. Samples G337-1 and G079-3 were not analyzed for presence of the GP2 protein due to scarcity of archived sera (figure 1). In two samples only, NP antigen could be detected at low levels (figure 1, G787-1, G090-3); however, GP2 analysis was not performed for the latter. In 34 patient and control sera, none of the three LASV proteins were detected, as outlined in the three examples in figure 1 (figure 1, G543-3, BOM011, BOM019). Identification of LASV proteins NP, GP1, and GP2 in patient sera was aided by comparing corresponding bands on VLP (figure 1, LASV VLP). The 42 kDa sGP1 component generated from expression of GPC in HEK-293T/17 cells was precipitated from the supernatant with PEG and salt and readily detected (figure 1, GPC 36 h). Viral proteins could not be detected in precipitated supernatants from cells transfected with plasmid vector (figure 1, pcDNA).
Figure 1. Western blot analysis of LASV antigen positive and negative patient sera and controls for GP1, GP2, and NP proteins. Twenty μL of a 1:4 dilution of precipitated suspected LASV patient sera were resolved per lane of a reducing SDS-PAGE gel, blotted and probed with α-GP1, α-GP2, or α-NP antibodies. In three samples NP, GP1, GP2 were detected, indicating presence of LASV virions (G692-1, G762-1, G765-1; NP, GP1,2 (+)). In G610-3, G676-A, G583-1, and G755-1 only GP1 was detected (GP1 (+)). Only low levels of NP were detected in G787-1 (NP (+)). Whereas in G337-1 and G079-3 only GP1 was detectable (GP1 (+)), GP2 levels were not determined (nd). Similarly, in G090-3 only low levels of NP were detected, but GP2 levels were not determined (nd). A representative suspect LASV patient serum sample that did not reveal detectable levels of all three viral antigens (G543-3) is shown alongside normal uninfected controls (BOM011, BOM019) (NP, GP1,2 (-)). In vitro controls derived from transfection of HEK-293T/17 cells with pcDNA vector (pcDNA) or a LASV GPC construct harvested at 36 hours (GPC 36 h) are shown. Soluble GP1 can be precipitated from the supernatants of cells expressing GPC (GPC 36 h). LASV VLP containing NP, GP1, and GP2 are shown for protein size comparison (L VLP) (in vitro ctrls). Molecular weights for each LASV protein are shown to the left of blots, and identified on the right.
In figures 2 and 3 western blot results were compared to available data generated with recombinant and traditional LASV antigen capture, IgM, and IgG assays. Figure 2A displays western blot data for GP1, GP2, and NP, whereas figure 3A lacks GP2 analysis. Antigen capture data using the traditional assay platform are displayed in figures 2B and 3B, and contain averaged A450 absorbance values for test patient, positive, and negative control sera. For each set of samples, the data were not normalized due to the lack of internal assay calibrators and analysis of patient sera on different dates concurrent with collection and analysis at the KGH. In these studies, presence of sGP1 as the sole detectable arenaviral antigen in serum samples was established only when both GP2 and NP could not be equally detected. Exclusionary parameters for sole detection of sGP1 were positive results in a traditional capture assay employing GP1, GP2, and NP-specific mAb cocktails, in a recombinant NP antigen capture platform, and detection of other viral components in western blots, as outlined in methods. The only two samples that met these criteria in the current studies were G610-3 and G755-1 (figure 1, 2A, 2B, 2C).
Figure 2. Antigen, IgG, IgM, and outcome profiles in suspected LASV patient sera from the first study (December 2009): G079-3 through G617-1. A. Trad Ag, IgG, IgM (USAMRIID), and r Ag, IgG, IgM (Hemorrhagic Fever Virus Diagnostics Consortium) were ELISA platforms, whereas GP1, GP2, NP WB were western blots. The USAMRIID assays were performed before the recombinant counterparts, sometimes months in advance. Western blot analyses were performed on samples shipped to the U.S. following irradiation. A very strong positive signal is indicated by ++ and a positive by +. Marginal positive detection is indicated by +/- and negatives by -. Gray boxes indicate that relevant data was not collected for the corresponding sample. Patient outcomes recorded in databases are: d, discharged; D, dead; na, not admitted; c, household contact of patient G676; ?, patient samples from Liberia, with unknown outcome. Non applicable (N/a) outcome is indicated for in vitro controls. B. Traditional antigen (Trad Ag) data showing the A450 O.D. for all samples (blue) alongside positive (dark red) and negative (green) controls. Data are plotted as mean ± SD, n = 2. Each sample in this series was analyzed on different days, concurrent with testing of suspect patients admitted to KGH. Collection dates for this series ranged from January 2008 to September 2009.
Figure 3. Antigen, IgG, IgM, and outcome profiles in suspected LASV patient sera from the second study (March and April 2010): G543-3 through G806-1. A. The legend and designations outlined in figure 2A above apply to figure 3. Sample G598-2 was duplicated in this series to verify previous results. B. Traditional antigen (Trad Ag) data as outlined for figure 2B above. Data are plotted as mean ± SD, n = 2. Each sample in this series was analyzed on different days, concurrent with testing of suspect patients admitted to KGH. Collection dates for this series ranged from June 2009 to March 2010.
In previous studies we described and characterized the phenomenon of LASV GP1 ectodomain shedding in vitro [1,2]. In order to investigate whether this phenomenon was specific in vivo, we analyzed serum samples from patients admitted to the KGH in Sierra Leone from 2008 - 2010 for the differential detection of LASV proteins. Our approach involved the detection of GP1, GP2, and NP in the same sample, with sensitive monoclonal and polyclonal antibodies. The detection of NP alone could be indicative of release of the protein from virally infected dead cells, and could conceivably remain in the bloodstream after viral clearance. This phenomenon has been observed in vitro [16,22]. Thus, acute viremia was characterized as the concomitant detection of all three viral proteins, GP1, GP2, and NP. The detection of GP2 implies its presence in the context of an enveloped virion, due to its known membrane-spanning properties via the transmembrane domain (amino acids 427 - 451 in LASV Josiah). Similarly, the presence of GP1 in the same samples would imply viremia because it associates with GP2 as a component of the non-covalently linked tripartite GPC complex. The nucleoprotein component of the virion should also be detected in all samples containing GP1 and GP2, thus confirming the presence of intact, enveloped, circulating Lassa virions. The detection of only GP1 in any given sample was interpreted as the absence of whole virions and the presence of the soluble form of the protein, as previously observed in vitro. The date and time of collection of any given blood sample represents a snapshot in the stage of a potential LASV infection. Within the context of an early acute viral infection, it is unlikely that a patient would present with symptoms immediately following exposure to the virus. Following viral infection of host cells and early replication events, the detection of sGP1 without accompanying progeny virions might be possible. This event may represent a very narrow window in the virus life cycle before the emergence of mature virions from infected cells in vivo. Thus, the ratio of samples where sGP1 alone was conclusively detected in the context of these studies was small (2/46). Following this very early step in viral biogenesis where host cells are secreting only sGP1, progeny virions will emerge from the surface of infected cells and will disseminate throughout body tissues and fluids. At this stage, differentiation of sGP1 from virion-associated GP1 is no longer possible. In the cases where any viral antigens, IgM and IgG were detected (G692-1, G762-1, G765-1), the possibility exists that a re-infection with clinical symptoms and development of febrile disease occurred. Two out of these three patients succumbed to Lassa fever; the outcome of patient G765-1 is not known. Each sample analyzed in these studies for LASV antigens was exhaustively subjected to western blot analysis with different viral protein-specific antibody reagents, at different serum dilutions, with extended exposures to sensitive films, and using an extensive panel of positive and negative controls to ensure that data were not the result of artefacts or background effects. Therefore, each sample was analyzed for presence of each antigen at least three times, either in a primary detection or in probing and reprobing formats. Although probing and reprobing allowed for the detection of antigens using a single blot, and thus preserving precious sample volumes, the membrane stripping process removes protein from the matrix, thus reducing the sensitivity of subsequent assays. The small volumes of available serum for analysis made further characterization of each sample unfeasible. Future studies will be aimed at characterizing the nature of shed sGP1 in vivo, namely proteolysis sensitivity and deglycosylation analyses, for which relevant in vitro data is available.
In these studies three sets of relevant data were used to interpret the antigenic status of the serum samples: 1. Traditional antigen (Trad Ag) ELISA; 2. Recombinant antigen capture, NP-specific; 3. Western blot analysis of GP1, 2 and NP. Direct correlations cannot be made between the three assay platforms based on several factors. The USAMRIID antigen capture assay employs two GP1-, two GP2-, and one NP-specific mAbs [24]. Thus, individual identification of each antigen cannot be established. Furthermore, this assay relies on a two-step signal amplification and detection, each with polyclonal antibody reagents, a rabbit secondary and a goat tertiary. The sensitivity of this assay, therefore, may be superior to the recombinant NP mAb-based antigen capture format, which employs a single detection step with a goat polyclonal raised against recombinantly expressed NP protein. Both antigen capture formats were performed with 1:10 dilutions of serum in assay buffer, in a total of 100 μL. Then, western blots were performed with precipitated protein from 5 μL of serum. Considering that each analytical format detects antigens in different conformations, direct data comparison is not feasible. Despite specificity and sensitivity issues at play, in 21/30 samples for which data is available from all three assay formats, the results from the Trad Ag and r Ag platforms agreed. In six samples, the Trad Ag assay detected LASV antigen and the r Ag platform did not. The higher sensitivity of the former may be due to the combination of 3 antigen-specific mAbs that detected LASV glycoproteins in addition to NP in the Trad Ag assay. Another possibility to consider is the quality of stored samples in less than ideal conditions over extended periods of time. The lack of continuous electrical power at the KGH over the years has resulted in temperature fluctuations in refrigerators and freezers, which may interfere with sample quality and stability of viral antigens. The time that elapsed between collection and analysis of samples used in these studies varied between 3 months and 2 years. Despite these technical issues, many samples that originally tested positive for antigen, IgM, and IgG, retain the specific binding pattern irrespective of the time elapsed since collection. A complete list of samples, dates of collection, antigen status, and known storage temperature are shown in Table 1. The two samples in which only sGP1 could be detected in these studies (G610-3 and G755-1) were collected in September 2009 and January 2010, and thus analyzed within 3 months of collection, in December 2009 and April 2010, respectively.
Table 1. Collection and sample processing dates for G-series samples
Nine samples tested positive for sGP1 by western blot (G079-3, G337-1, G583-1, G610-3, G676-A, G692-1, G755-1, G762-1, G765-1). In three additional samples, the r Ag assay marginally detected NP in serum samples (G652-3, G803-1, G803-2). However, these three samples did not test positive for sGP1 or NP by western blot. Our data suggests that a NP-based Ag capture assay currently in use has a sensitivity threshold of 1.5 ng rNP/mL (unpublished data). Western blots have not thus far successfully detected NP Ag in the low ng/mL level. Three other samples tested positive in the Trad Ag and r Ag ELISA assays, along with detection of sGP1 by western blot analysis, but did not test positive for NP by western blot, and were, therefore, not considered examples of glycoprotein shedding (G079-3, G337-1, G583-1). In this group of samples only G583-1was analyzed for presence of GP1, GP2, and NP, whereas the remaining two were not tested for GP2. Despite the absence of GP2 by western blot, the presence of NP by antigen capture ELISA and the fact that it was positive by the traditional antigen capture ELISA made it impossible to distinguish between the phenomenon of sGP1 shedding and the presence of GP1 in enveloped virion format. Thus, we have considered samples G610-3 and G775-1 as the only examples of clear LASV GP1 shedding in these studies, though the phenomena of GP1 shedding may occur throughout viremia. In both samples, LASV antigen was not detected by Trad Ag and r Ag ELISA, and western blot did not reveal presence of GP2 and NP. However, GP1 was clearly present (figure 1, G610-3, G755-1). For sample G676-A, collected from a household contact of patient G676 who succumbed to Lassa fever, Trad Ag and r Ag ELISA data were not available; therefore, this sample was also not considered a clear example of GP1 shedding.
Based on the proposed narrow window of sGP1 detection in vivo likely to occur in the first few days after primary infection with LASV, it is not surprising that only 2/46 samples analyzed in these studies would not show any other viral proteins indicating an early LASV infection. The known incubation period for infection with LASV is approximately 6 - 7 days [24,25]. However, admission to local hospitals is usually delayed following the onset of febrile disease compounding the low detection rate of shed GP1. Thus, in many cases, patients admitted to the KGH present with acute viremia, or undetectable antigen but rising IgM titers, indicative of advanced LASV infection.
Strecker et al., 2003 [19] reported the stoichiometric ratio of NP:GP1:GP2 in Lassa virions as 160:60:60. In the context of a LASV virion, detection of one protein should result in the detection of the other two. Thus, detection of GP1 without concomitant detection of either GP2 or NP can be interpreted as a secreted isoform of GP1 prior to but not necessarily limited to the emergence of enveloped viral particles from infected cells, that likely corresponds to the sGP1 component identified in vitro (figure 1, GPC 36 h) [1,2]. Although the possibility exists that sole detection of sGP1 in serum samples is the result of degraded virions or viral protein released from infected cells, recent studies with LASV VLP suggest differently. The structure and function of arenaviral glycoproteins have resulted in significant resistance to proteolytic digestion with highly active proteases, namely trypsin and Proteinase K [26]. We and others have observed that glycosylated LASV GP1 and GP2 are highly resistance to the effects of proteolytic digestion, whereas NP and Z are not. Thus, degraded virions that afford detection of GP1 should also result in equivalent detection of GP2, despite the loss of NP (and Z). Moreover, lysis of infected cells in vivo would parallel the effects of in vitro LASV infection, but selective release of the GP1 protein is highly unlikely. If GP1 generated during viral biogenesis is released from lysed cells, concomitant detection of GP2 and NP should be observed. Generation of LASV VLP in mammalian cells via transfection with GPC, NP, and Z gene constructs resulted in pseudoparticles containing all three proteins closely associated in enveloped structures. Additionally, sGP1 and a minor soluble NP fraction, but not Z or GP2, could be detected in the least dense sucrose fraction in gradient centrifugation assays, indicating their lack of association with enveloped pseudoparticles [26]. Release of NP from dead cells was observed in the studies by Branco et al. [26] and by and others [22]. Conversely, soluble GP1, but not GP2, could be readily detected in the supernatant as early as 12 hours after transfection with GPC constructs in vitro. Release of NP from transfected cells was not observed in the context of single gene transfections [26]. Together, these results strongly suggest that a soluble GP1 component can be secreted from cells expressing the LASV glycoprotein complex prior to release of mature virions in vivo. A graphic model outlining a proposed temporal progression of virus infectivity of permissive cells, glycoprotein display and sGP1 shedding, relevant protein components in LASV virions, and release of viral components from dead cells is shown in figure 4.
Figure 4. Graphic model of glycoprotein display and sGP1 shedding, relevant protein components in LASV virions, and release of viral components from dead cells. (1) LASV glycoprotein mediates virion binding to alpha dystroglycan (αDG) on permissive cells (1a). Following receptor binding the virion fuses with the host cell membrane, resulting in viral entry. Viral replication takes place in the cytoplasm of the infected cell. (2) During replication and before mature virions are assembled GPC is proteolytically processed and a portion of the resulting GP1 is transported to the cell surface and shed as sGP1 (2a). Concurrently, GPC is expressed on the cell surface, mediated by GP2 anchoring in the bilipid membrane, non-covalently associated GP1, and the hydrophobic signal peptide (2b). (3) Budding virions emerge from the cell surface mediated by Z matrix protein as enveloped particles containing membrane lipid and proteins, viral GPC and nucleoprotein. (4) Virions are released from infected cells containing nucleoprotein and associated viral RNA segments, along with non-displayed polymerase and cellular ribosomes. Budded virions will enter the circulation and can be detected in whole antigen capture assays or by western blot identification of individual proteins, as performed in these studies. Presence of intact virions should permit the identification of GP1, GP2, and nucleoprotein. (5) Nucleoprotein and glycoproteins can shed from dead or dying cells, with compromised cell membrane integrity. This phenomenon is observed in vitro, and may occur in vivo, hence the detection of only NP in some serum samples. A graphic representation of each relevant component in the outlined pathway is shown in the legend box. EC: extracellular; IC: intracellular; CM: cell membrane.
Through this work, we have identified a soluble form of LASV GP1 in the serum of infected patients. Although the exact stage of viral infection in all patients could not be determined through these studies, the lack of detection of the virion associated NP and GP2 proteins, with clear identification of GP1 in the serum of acutely infected individuals, points to an early event in viral replication when only sGP1 can be detected. Although a role for sGP1 in arenaviral infections in vivo has not been established, future work will attempt to characterize functional roles for this protein. It is likely that LASV sGP1 performs immunomodulatory and decoy functions similar to those proposed for EBOV, a filovirus; respiratory syncytial virus (RSV), a paramyxovirus; human immunodeficiency virus (HIV), a retrovirus; and rabies virus (RABV), a rhabdovirus. In EBOV and RSV infections it has been proposed that ectodomain shedding of the viral envelope glycoprotein serves as an immune decoy, with significant enhancement of pathogenicity in vivo [3,27].
Definition of the role(s) of LASV sGP1 in vivo could lead to new correlates of the disease, opportunities toward development of diagnostics targeting early events in acute infection and viral biogenesis, and the ability to counter potential viral immunomodulatory pathways that confer poor outcomes.
Human serum collection
Febrile patients suspected of having LF were admitted to the LF Ward at the Kenema Government Hospital (KGH) in Kenema, Sierra Leone. Informed consent was obtained and each patient was assigned a unique identification number (G-XXXX). Approximately 5-10 ml of blood was collected from each patient. Blood was collected into 5 ml serum collection tubes using butterfly safety needles. The same procedures and assurances were employed in the collection of blood from normal human volunteers. Normal human sera used in these studies were collected in the Bombali district of Northern Province, Sierra Leone, and designated BOM.
Precipitation of total protein from human serum samples
Serum samples collected from patients admitted to the KGH Lassa fever ward (G-series), household contacts of hospitalized subjects (G-series-A), and individuals not known to have had Lassa fever (BOM) were aliquoted and stored in cryovials at the KGH Lassa fever laboratory. Samples used in these studies were visually inspected for presence of follicular precipitate, coagulate, significant discoloration, contamination, and haemolysis. Only clear serum samples were used. Twenty μL of each serum sample were diluted 5-fold with sterile D-PBS, pH 7.4 and combined with 20% polyethylene glycol-6000 (PEG-6000) and 2 M NaCl stock solutions to final concentrations of 5% and 0.2 M, respectively. Samples were incubated at 4°C overnight, followed by centrifugation at 21,000 ×g, for 75 minutes at 4°C. Supernatants were carefully aspirated and discarded. Pellets were resuspended in SDS-PAGE buffer with 50% glycerol, heated without reducing agent, and stored frozen until shipment. Samples were shipped to the U.S. in IATA-approved containers and were irradiated with 2500 KRad upon arrival, using a Cs source. Recombinant LASV VLP expressing Z+NP+GPC were used as controls for identification of viral proteins in SDS-PAGE, along with soluble GP1 (sGP1) from HEK-293T/17 cells transfected with a wild type GPC gene. The generation of LASV VLP and sGP1 have been described elsewhere [1,2,26].
Western blot analysis
Four-fold dilutions of protein sample from a 20 μL serum aliquot were prepared in SDS-PAGE sample buffer, reduced with DTT, heated to 75°C for 10 minutes, and resolved on 10% NuPAGE Novex Bis-Tris gels, according to the manufacturer's specifications (Novex, San Diego, CA). Proteins were transferred to 0.45-μm nitrocellulose membranes, blocked, and probed in 1X PBS, pH 7.4, 5% non-fat dry milk, 1% heat inactivated fetal bovine serum, 0.05% Tween-20, and 0.1% thymerosal. Detection of LASV GP1, GP2, and NP in precipitated protein from human serum samples was performed by Western blot analysis using anti-LASV mAbs L52-74-7A (GP1), L52-216-7 and L52-272-7 (GP2), and goat PAb to E. coli generated nucleoprotein, respectively. Secondary antibodies were horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L) or rabbit anti goat IgG (H+L). Membranes were then incubated in LumiGlo chemiluminescent substrate (KPL) and exposed to HyBlot CL Film (Denville Scientific, Inc). Blots used in reprobing experiments were briefly rinsed in PBS-T (1X PBS, pH 7.4, 0.1% Tween 20) after exposure to x-ray film, followed by incubation in stripping buffer (62.5 mM Tris, pH 6.8, 2% SDS, 100 mM β-ME) for one hour at 65°C. Blots were then washed extensively in PBS-T, re-blocked, and reprobed as outlined above. Blots were reprobed a maximum of three times.
Competing interests
Luis M Branco, Frederick J Geske, and Robert F Garry are listed inventors, in addition to others, in a PCT application entitled "Soluble and Membrane-Anchored Forms of Lassa Virus Subunit Proteins", filed in April 2008. Additionally, Luis M Branco and Robert F Garry are listed inventors in a provisional application for United States letters patent entitled "Lassa virus particles and methods for production thereof", filed in September 2009. This work was performed as partial fulfilment of Ph.D. dissertation requirements for Luis M Branco and Jessica N Grove.
Authors' contributions
LMB and JNG contributed to the experimental design, performed sample analysis, interpreted data, and drafted the manuscript. LMM coordinated blood sample collection from LASV convalescent individuals according to established IRB protocols, managed the KGH outreach team, and helped process samples. AG, MF, and MM coordinated blood sample collection from convalescent individuals and from suspected Lassa fever patients at the KGH, performed and managed the analysis of sera by traditional antigen, IgM, and IgG assays. RJS kindly provided monoclonal antibodies to relevant LASV proteins, and critically reviewed the manuscript. DB provided critical review of the manuscript. RFG contributed to the experimental design and provided critical review of the manuscript.
This work was supported by Department of Health and Human Services/National Institutes of Health/National Institute of Allergy and Infectious Diseases Challenge and Partnership Grant Numbers AI067188 and AI082119, and RC-0013-07 from the Louisiana Board of Regents. The research described herein was sponsored in part by the Division of GEIS Operations at the Armed Forces Health Surveillance Center, Research Plan C0169_10_RD. We thank the members of the Hemorrhagic Fever Diagnostic Consortium, and Lassa Fever - Mano River Union for ongoing support. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the U.S. Army.
Illick MM, Branco LM, Fair JN, Illick KA, Matschiner A, Schoepp R, Garry RF, Guttieri MC: Uncoupling GP1 and GP2 expression in the Lassa virus glycoprotein complex: implications for GP1 ectodomain shedding.
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Branco LM, Garry RF: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms.
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Fisher-Hoch SP, McCormick JB: Lassa fever vaccine: A review.
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McCormick JB: Epidemiology and control of Lassa fever.
Fisher-Hoch SP, Tomori O, Nasidi A, Perez-Oronoz GI, Fakile Y, Hutwagner L, McCormick JB: Review of cases of nosocomial Lassa fever in Nigeria: the high price of poor medical practice.
McCormick JB, Webb PA, Krebs JW, Johnson KMES, Smith ES: A prospective study of the epidemiology and ecology of Lassa fever.
J Infect Dis 1987, 155:437-444. PubMed Abstract
McCormick JB, King IJ, Webb PA, Scribner CL, Craven RB, Johnson KM, Elliot LH, Belmont-Williams R: Lassa fever. Effective therapy with ribavirin.
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Lenz O, ter Meulen J, Klenk HD, Seidah NG, Garten W: The Lassa virus glycoprotein precursor GP-C is proteolytically processed by subtilase SKI-1/S1P.
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Gallaher WR, DiSimone C, Buchmeier MJ: The viral transmembrane superfamily: Possible divergence of arenavirus and filovirus glycoproteins from a common RNA virus ancestor.
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Young PR, Chanas AC, Lee SR, Gould EA, Howard CR: Localization of an arenavirus protein in the nuclei of infected cells.
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Hufert FT, Ludke W, Schmitz H: Epitope mapping of the Lassa virus nucleoprotein using monoclonal anti-nucleocapsid antibodies.
Arch Virol 1989, 106:201-212. PubMed Abstract | Publisher Full Text
Perez M, Craven RC, de la Torre JC: The small RING finger protein Z drives arenavirus budding: implications for antiviral strategies.
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Strecker T, Eichler R, Meulen J, Weissenhorn W, Dieter Klenk H, Garten W, Lenz O: Lassa virus Z protein is a matrix protein and sufficient for the release of virus-like particles.
J Virol 2003, 77(19):10700-10705. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Eichler R, Strecker T, Kolesnikova L, ter Meulen J, Weissenhorn W, Becker S, Klenk HD, Garten W, Lenz O: Characterization of the Lassa virus matrix protein Z: electron microscopic study of virus-like particles and interaction with the nucleoprotein (NP).
Virus Res 2004, 100(2):249-255. PubMed Abstract | Publisher Full Text
Strecker T, Maisa A, Daffis S, Eichler R, Lenz O, Garten W: The role of myristoylation in the membrane association of the Lassa virus matrix protein Z.
Virol J 2006, 3:93. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text
Schlie K, Maisa A, Freiberg F, Groseth A, Strecker T, Garten W: Viral protein determinants of Lassa virus entry and release from polarized epithelial cells.
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Ruo SL, Mitchell SW, Killey MP, Roumillat LF, Fisher-Hoch SP, McCormick JB: Antigenic relatedness between arenaviruses defined at the epitope level by monoclonal antibodies.
J Gen Virol 1991, 72:549-555. PubMed Abstract | Publisher Full Text
Bausch DG, Rollin PE, Demby AH, Coulibaly M, Kanu J, Conteh AS, Wagoner KD, McMullan LK, Bowen MD, Peters CJ, Ksiazek T: Diagnosis and clinical virology of Lassa fever as evaluated by enzyme-linked immunosorbent assay, indirect fluorescent-antibody test, and virus isolation.
J Clin Microbiol 2000, 38:2670-2677. PubMed Abstract | PubMed Central Full Text
Jeffs B: A clinical guide to viral haemorrhagic fever: Ebola, Marburg, and Lassa. Trop Doct.
2006, 36(1):1-14. PubMed Abstract | Publisher Full Text
Branco LM, Grove JN, Geske FJ, Boisen ML, Muncy IJ, Magliato SA, Henderson LA, Schoepp RJ, Cashman KA, Hensley LE, Garry RF: Lassa virus-like particles displaying all major immunological determinants as a vaccine candidate for Lassa hemorrhagic fever.
Virology J 2010, 7:279. BioMed Central Full Text
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Lenz O, ter Meulen J, Klenk HD, Seidah NG, Garten W: The Lassa virus glycoprotein precursor GP-C is proteolytically processed by subtilase SKI-1/S1P.
Proc Natl Acad Sci USA 2001, 98(22):12701-12705. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Urata S, Noda T, Kawaoka Y, Yokosawa H, Yasuda J: Cellular factors required for Lassa virus budding.
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Automatically replies to the caller via SMS based on the mode selected. The objective of this Android application is to free the user of replying in difficult situations (like driving) or to enable the phone of answering when it is not with the user (e.g. the user is bathing etc).
Birthday Greeter is an Android app which automatically greets your Facebook friends birthday by sending a personal message, predefined by you to your friends' Inbox on the day of their birthday! Birthday Greeter alerts you every time it sends a message to your friends, so you can always track your birthdays and also add the extra personal touch and write on their wall. No worries, Birthday Greeter works even if you are offline, you will never miss a friend's birthday!
Ever wished your phone calls could be automatically recorded? That conversation you recently had with your sweetheart, The deal you recently made, Vital information you keep forgetting, Great moments shared with your friends and family. Rejoice, Automatic Call Recorder is now available on your device. The only call recording app that works on every android phone and tablet. Why use automatic call recorder: Auto record outgoing/Incoming calls: A unique and smart feature that automatically records outgoing or incoming calls or both. Contacts to record: You can choose particular contacts to record. Contacts to ignore: You can choose particular contacts to ignore recording. Excellent Audio Quality: Say no to poor audio quality.
A very simple to use Automatic Call Recorder app. Just install and forget. Each call whether incoming or outgoing will be recorded seamlessly. An intuitive interface ensures that the calls recorded are displayed in a list with inbuilt media player. The recordings can be setup automatically to be backed up. Features: 1. Auto recording incoming and outgoing calls 2. You can see call details for particular recorded calls 3. Enable/Disable auto call recording. 4. Turn on/off notification while recording call. 5. You can manage count of recording calls. 6. You can change file type of call to be recorded. 7. Manage black list if you want to ignore recording for particular contacts. 8. Mange audio source. 9. You can ignore all contacts or can ignore black listed contacts for auto recording. 10. Mange ignore contact list. 11. Auto Sync.
Record any outgoing or incoming calls you want. Full SD Card support. Choose what you want to save. Share your calls with friends or store on dropbox and upload to cloud. Full call history with date and time. You can even choose the format you want for your recorded calls. It is easy to use with full pro capabilities. You can save any number of calls you want. On some handsets call recording may not be supported. This is not a problem with the app but the phone manufacturer chose to not allow the function.
Smart Caption for Youtube: YouTube Video with Caption. The Smart Caption is all in one video player for YouTube Caption. Also use it as a movie player. The captions are automatically organized. Also Native language and English can be used simultaneously as using both subtitle on layer screen. Use the Smart Video for learning and more fun anywhere in a short time. Smart Video Features: Easy video and subtitle management using gestures, Various learning methods: List subtitle, Video repeat, Subtitle vocabulary, Automatic translation, Easy to use, Touch: Run Video and subtitle.


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Looking for something cool and unique? We have a great selection of "that's so cool" and "where did you get that" items!
This colourful, wood-framed magnetic board is a fun way of keeping track of your favourite team's progress during the hockey season! Included are colourful puck magnets for all 30 NHL teams, separated into each of the 4 divisions in the NHL.
Mount the board on your wall using the rear hooks, then rearrange the magnets into 1st through last place as the season progresses, and move the magnets to the Stanley Cup section of the board when playoff action starts! Who will win the Cup? You'll be able to track the action with this countdown to the Cup, Magnetic NHL Standings Board!
Measures approximately 23" long x 14.25" high.
It's that time of year, our 2014
Gift Guide is here! It's full of wonderful gifts and awesome deals to
jump-start the season of surprises and holiday delights. We know the way kids
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We are thrilled to announce the opening of our new store in Coquitlam, British Columbia!
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#SUPERCONDUCTOR : The Local HOption :
http://twitter.com/#!/JUNCTIONCRAFT :
The Local HOption
by EarMAP
Tote Bag
Our quality crafted Tote Bags are hand sewn in America using durable, yet lightweight, poly poplin fabric. All seams and stress points are double stitched for durability. Available in 13" x 13", 16" x 16" and 18" x 18" variations, the tote bags are washable, feature original artwork on both sides and a sturdy 1" wide cotton webbing strap for comfortably carrying over your shoulder.
- 8Promote


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Faith is the bird that feels the light and sings when the dawn is still dark.
- Rabindranath Tagore
Celebrate endings - for they precede new beginnings.
- jlh
I decided today I would blog just once more before the new year. this past year has seen many ups, downs, and so many inbetweens. I'm ready for the new year to begin so I can have a fresh start, fresh ideas, and fresh goals. Wait.........I can have that every day of the year. I could but sometimes I don't. The new year always brings with it the spark of new things to come, clean fresh starts, new goals and new resolutions. Some are kept some are forgotten long before they even begin. I have made two: reach my goal weight, and get myself on track with my budget. I'm getting there with the budget, which may I add is a lot like a new healthy lifestyle. You have to cut somethings, add others, and keep on track daily. I've kept it simple, im following the plan both in my budget and in my lifestyle where food, exercise and water are concered.
So I end this old year with so many new plans for the new year. I didn't even have to wait for the new calender, I began the beginning of the week. Good luck to you all in the coming year. Clean out the old and bring in the new.
As this year draws to a close i'm able to look back and see where i've come from and now look forward to where I want to go. I have so many new wishes for the coming year. I want to see my "magic number" appear in the scale window, I want to shake off this funk i've been in now for a bit, I want to see my daughter married May 7th, I want to watch my grandson become the wonderful little boy he is turning into. I want to be happy. I"m not looking for riches, or gold, I just want to be happy. I guess that is all any of us can ask for at any given time. we just have to work at it. Happiness comes so easy to some and the rest of us have to really work at it. I'm learning to see my blessings each and everyday. I write of them in my journal daily. Helps me to see i've been blessed.
So as I approach this new year with so much excitement and the plans I have for the coming year, i'm blessed to have spark, i'm blessed to have Weight watchers, I'm just plain blessed in this journey. I have made so many friends, they help to hold me up, and give me so many great ideas for the new me i'm becoming. After all we are all works in progress as this juncture in our lives. None of us is perfect yet. we all have a ways to go to get there, but i'm proud to walk this path with all of you.
I sat down this morning and wrote my goals out for the coming year. I have my plan in place and with a little help from my friends I will hope to reach a couple of them. The plan is pretty easy, mostly same ole same ole. I need to exercise, drink my water, get my fruits and veggies, my rest. the rest should fall into place once i've taken care of me.
So today I begin my new journey for the new year. I have so far to go and really i'm right there. the journey is funny like that, it can lead you all over or right next door.
I will post a few pics hopefully this weekend. My daughter borrowed my camera as hers won't work. So I don't have it to download what we took. My grandson was awesome this year. we do it where he gets a gift, then it is someoneelses turn for a gift and so on. He waited his turn, and as he gets wayyyy more then us we give him a second in a row, he stopped us and told us it was his mommas turn for a present. I was so proud of him. He is so grown up sometimes. we told him he couldn't do gifts untill we all finished with dinner, when we where done he came to each of us in turn to ask if we were finished. It is amazing how grown up a three year old can be. I'm truly blessed this holiday.
I also will have a pic of my "Christmas tree of decorations". I look at it and can't believe all the good days I had. they were not all in a row but I got a lot of "stars" for my tree. I have a new calender for the new year and smiley faces this time. I'm going to succeed one moment at a time. I think this is a lot about us as we grow and about the losses. sometimes I forget that and having a visual helps me to see that really i'm doing great. I have bad days, but in the end i'm holding my own. I"m not losing like I would hope, but i;'m losing, i'm staying in the same weight range when i don't. I need to start seeing the glass as half full instead of always half empty.
So as the new year approaches i'm hoping for a sence of renewal. Rebirth so to speak. A fresh year, with fresh starts, fresh ideas and outlooks. What more do we need? nothing.
I want to wish all a very Merry christmas this holiday season. Be blessed in all that you do.
I refuse to go quietly into that good night. I"m down 0.2 this week. I worked hard for that. I was expecting a bit more. But i"m not giving up or in, i'm not going quietly and i'm not ready to quit. My weight doesn't have me yet. I'm actually down 2 inches total in the last month. took those measurements today also. So even though my weight is plugging along my body is taking on shape, definition and soon the weight will come with it. So today Christmas eve begins my new week. Week three on the new weight watchers program. I"m not seeing a huge change in my weight from it but i'm still working out the kinks. At least that is what I like to tell myself. I'm going to win this battle of the bulge if it takes me another year to do it. I wanted to be at goal by years end, that isn't going to happen, but in a glass half full kinda way i'm down another 5 pounds this year. I know that sounds really lame, but when your close to goal, stop and start as many times as I do, stress, holidays now, well it all works to hold us back. And if I'm truthful with you all and myself it is probably because i'm getting settled at this weight. I like the size i'm at, I like the way I look. I know it isn't in the healthy BMI range yet, and it isn't goal, but I'm ok here for the time being. Is this the weight I want to be? Nope. I'm still holding out for that magic number, so if it takes me another year, Oh well at least it will stay off that way.
So I give it my all not to get too upset over these things. Not that I don't, I feel like I give it my all and then to step on the scales and loose a tiny amount is really disheartening. but again i'm doing my best to see that i'm making headway. Rome wasn't built in a night and I guess this weight is going to come off easy. Some good things are worth fighting for. So I will sit down once again, plan my week stratagey, change up a few things, and then give it heck. There is still next week.
Happy holidays to you all and be blessed.
Life is a succession of lessons which must be lived to be understood.
- Helen Keller
Self-pity is our worst enemy and if we yield to it, we can never do anything wise in this world.
- Helen Keller
I recieved a call from my son whom lives in vermont yesterday. As some of you may know we haven't been on the best of terms. He is a troubled young man and sometimes being mom and being the one that he lashes out at, well we haven't been on the best of terms. Anyhow I recieved an email which turned into a phone call. My son lost his job about two months ago and even though he said it wasn't his fault.....well I didn't believe him. As an addict can do he lies a bit more than usual. So you really have to take what he says with a grain of salt and move forward. I know that sounds a bit harsh from a parent but once burned twice shy....anyhow he had been fighting for it back. Went to all the available chanels and he won. He had witnesses and managers in his corner. He won that battle. He is at a different location but he is back to work. As a parent i'm so proud he took it upon himself to say "hey wait a minute I was wronged", he actually stood up for himself and he won. I will be proud for him and hope he is growing up a bit. we talked, he listened, I listened. Who knows maybe this is a step for both of us in the healing prosess. Oh it may take a lot more truths to be revealed on my part, but i'm going to be alright and I pray each day for him. Like I tell him, it is one day, one step at a time.
He is taking steps to help himself and i'm taking steps to help me. I'm getting healthier, heck I am healthier, i'm learning to relax, and i'm trying not to be the one to fix the worlds troubles. I did tell my son that I can't be held responsible for his choices. See that word comes into play alot, choices, anyhow, He made the choices with the friends he chose, I didn't. He had the same opportunities as his sister, he chose the path he is traveling. You can't go back but you can always move forward. I'm moving myself forward. each choice I make for me is a choice I make to further my life, my health, my happiness. It again boils down to our choices.
So yesterday that one phone call gave me a gift, a prayer answered. It can't be wrapped but it arrived in the same pretty packaging as all the rest......my beautiful baby boy is becoming a man.


text:
manageSaltos de línea: man¦age
Pronunciación: /ˈmanɪdʒ /
Definición de manage en inglés:
1 [with object] Be in charge of (a business, organization, or undertaking); run: their elder son managed the farm
Más ejemplos en oraciones
- Focus on experiences pertinent to operating and managing a farm business as well as community activities that you and your family are involved in.
- The business is managed by son Robert and daughters Helen and Jane.
- The elder son Ashok manages the business while the younger son helps him.
1.1Have the position of supervising (staff) at work: the skills needed to manage a young, dynamic team
Más ejemplos en oraciones
- I have just been promoted to supervisor in an insurance-claims office, managing a staff of 15.
- Mr Main has been head chef there for two years, managing a kitchen staff of 16.
- The sisters discovered that managing a staff of 15 with entrenched work practices was not easy.
1.2Be the manager of (a sports team or a performer): he managed five or six bands in his career
Más ejemplos en oraciones
- He acknowledged the input of Stephen Dormer who had assisted him in managing the team but who was unable to be present this week.
- Richie Bell was managing the senior team up until the time of his death in February 1994 and his accomplishment of winning three All-Ireland titles in a row by that stage was difficult to follow.
- Andy Deery is managing the team and anyone who would like to play should ring him.
1.3Use (money, time, or other resources) sensibly: we manage our cash extremely well
Más ejemplos en oraciones
- Free access to additional borrowings can make life easy for those who manage their money sensibly, but prove a disaster for spendthrifts.
- This should serve as a grim warning to those poised to embark on their university careers of the need to manage their money sensibly.
- The back office mobilises financial resources and manages funds.
organize, take care of, administer, regulate, deal with efficiently
1.4Maintain control over (a person or animal): she manages horses better than anyone I know
Más ejemplos en oraciones
- They were spooked, but found a way to manage him.
- His mother cannot manage him due to his problems and there were also fears for his safety in the community if he was released, the court had also heard.
- It broke my heart to put her in there but doctors told me I wouldn't be able to manage her.
1.5Control the use or exploitation of (land): the forest is managed to achieve maximum growth
Más ejemplos en oraciones
- If family forest owners can convince the public that they are managing their lands for long-term sustainability, they believe that can lead to reduced regulations.
- In exchange, the landowners manage their land to provide habitat for the desired species.
- Family forest landowners (often known as tree farmers) take pride in managing their lands.
2 [no object] Succeed in surviving or in achieving something despite difficult circumstances; cope: Catherine managed on five hours' sleep a night
Más ejemplos en oraciones
- You can get the best of Middle Eastern, especially Egyptian, spices and spice mixes in the dingy shops where you can manage with Hindi.
- As James Marshall Bridge states, more has to be shared, so it stands to reason that Joe Public has to manage with less as there will be less to go round.
- It does mean that we are going to have to rent out a base in the centre of town for about a month around festival time, but we'll manage with that.
cope, get along/on, make do, be/fare/do all right, carry on, survive, deal with the situation, scrape by/along, muddle through/along, fend for oneself, shift for oneself, make ends meet, weather the storm
2.1 [with object] Succeed in achieving or producing (something difficult): she managed a brave but unconvincing smile [with infinitive]: Blanche finally managed to hail a cab
Más ejemplos en oraciones
- While these tasks can be managed, it is difficult to do so effectively and in a timely manner.
- Overall, the new President can be said to have managed a fairly difficult balancing act.
- He tried to find his voice, but it was difficult for him to manage anything but a feeble sounding grunt.
accomplish, achieve, do, carry out, perform, undertake, bring about/off, effect, finish, succeed in, contrive, engineer
2.2 [with object] Succeed in dealing with or withstanding (something): there was more stress and anxiety than he could manage
Más ejemplos en oraciones
- Those suffering from Tourette's need help to develop strategies for dealing with and managing their tics, and, where necessary, medical treatment.
- But I had managed to get a supply of special tablets that turn your stomach to concrete, so I could manage the flight.
- As a footnote, I would reluctantly object to the consumption of food in the mall, but this could easily be dealt with in the same way the shops manage the problem.
Definición de manage en:
- Inglés de EE.UU. dictionary
- English synonyms
- US English synonyms
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Palabra del día vicarious
Pronunciación: vɪˈkɛːrɪəs
experienced in the imagination through another person's feelings…


text:
November 28, 2014
KNOXVILLE, Tenn. (WVLT)-- The Webb School brings up-to-date sports coverage of their high school games through the internet and it's all put together by students.
It's called the W.O.W Network and stands for "Webb on the Web". It's a broadcast that starts with a pre-game show, a play by play commentary during the game, and live interviews after the game.
It's a club of high school and middle school students who cover the games just like the professionals do on television.
Charlie Graham, an Analyst and Color Commentator said "We try to treat each team equally and try not to be fans of either team. We might be young but we're very serious about our broadcasts."
The students do everything from the press-box and broadcast across the internet; something that other schools aren't doing.
"It's such a great opportunity for all of us. I'd like to pursue broadcasting in college and working with the W.O.W network has really helped me understand what broadcasting is all about." said Myan Patel an Analyst for the games.
It takes plenty of time and dedication to pull this broadcast off every Friday but the students are willing to work hard for something they enjoy.
Andrew Robinson a play-by-play announcer said "When you are passionate about something time isn't really on your mind. I'm up here it's like I'm in my own little hemisphere and there's nothing better to me than getting up here on Friday nights reporting on sports."
You can listen to their broadcast online as they head into the State Semi Finals against Donelson Christian Academy; on Friday November 16 by clicking on the link below.
Click Here to access the online Public Inspection File
Viewers with disabilities can get assistance accessing this station's FCC Public Inspection File by contacting the station with the information listed below. Questions or concerns relating to the accessibility of the FCC's online public file system should be directed to the FCC at 888-225-5322, 888-835-5322 (TTY), or firstname.lastname@example.com.


text:
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C.- WOHM, CPST Instructor, and all around busy Mama to A.- 02/04, I. 01/07,E. 09/10 and
expecting the surprise of our lives Fall 2012!
Originally Posted by haydensmom1
YES! I have to be very careful with the tone I use with him. I know it's because I'm not feeling good and I'm so tired. Bless his heart! I have to make a concious decision to be very patient with him many times a day. We skipped the terrible twos and am living large in the TERRIBLE THREES. I keep telling myself he is just going through a phase and I will feel better in a few weeks.
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Articles Tagged 'Day 1 Studios'
Wargaming.net Announces World of Tanks: Blitz
By Ray Carsillo, Reviews Editor
Posted on April 1, 2013 AT 12:35pm
Posted on April 1, 2013 AT 12:35pm
At GDC last week, we had the opportunity to sit down with representatives from Wargaming.net as they unveiled the latest addition to their ever-growing universe in World of Tanks: Blitz. READ MORE
Prototype Footage For Gotham By Gaslight Emerges
By Eric L. Patterson, Executive Editor
Posted on February 27, 2012 AT 04:05pm
Posted on February 27, 2012 AT 04:05pm
Remember Gotham by Gaslight, the pitch for a steampunk-inspired Batman game that surfaced a while back? Now, prototype animation footage for the proposal has been revealed. READ MORE
Day 1 Studios Layoffs
By Jason Almenas
Posted on December 3, 2011 AT 09:11am
Posted on December 3, 2011 AT 09:11am
Sad news broke yesterday when we learned that F.E.A.R 3 developer Day 1 Studios announced they laid off 95 percent of its Chicago based development team. READ MORE
Today's Top 10 Stories
343 apologizes for Halo: The Master Chief Collection issues, promises to 'make this right with our fans'
Partner Pages
Top Partner Stories
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Festivals & Concerts
- Halloween Festivities
- Black History Month
- CommUNITY Picnic
- Day In The Life
- Ethnic Arts Festival
- Green Living Festival
- Hispanic Heritage Month
- Holidays
- Kits and Cats
- Lakeshore Arts Festival
- Martin Luther King Jr. Day
- Starlight Movies & Concerts
- Streets Alive! Festival
Ethnic Arts Festival
The Evanston Ethnic Arts Festival is a celebration of arts and cultural diversity unlike any other!
Save the date: Next year's festival will take place July 18-19, 2015.
Interested in performing at a City of Evanston summer concert or festival? Interested in being an artist or food vendor at Evanston's special events? Application forms for the 2015 season will be posted here on January 5, 2015.
July 19-20, 2014
Saturday & Sunday, noon to 7 p.m.
Centennial Park, Sheridan Rd. at Clark St.
Free admission.
Note: due to construction at the Arrington Lagoon in Dawes Park, the festival will move just north to Centennial Park, Sheridan Road at Clark Street. Free parking will still be available just north of the festival grounds (and will be even closer this year!) and free shuttle buses will again be in service.
Evanston's lakefront will be transformed into a Global Village this weekend for the 29th annual Evanston Ethnic Arts Festival, a two-day celebration of global diversity. This free event features art from an abundance of countries, family crafts activities, international cuisine, and live music, dance, and spoken word performances. Attendees experience the colors, sounds, and aromas of world cultures -- they can learn a folk-dance from a faraway country, and experience the intriguing spices and seasonings of new ethnic foods.
Join us as the Evanston Escola de Samba kicks off the 29th Annual Evanston Ethnic Arts Festival with a high-energy show of Brazilian percussion and a Carnival Parade. Check out their performance from last year's 4th of July Parade.
Attendees will enjoy continuous performances throughout the weekend at two venues. This family-friendly event also features a wide range of crafts activities throughout the weekend. Activities include making aztec headdresses, face painting, mehndi body painting, origami, indigo and natural dyes, and much, much more. View the 2014 activities here.
The Ethnic Arts Festival is sponsored by the City of Evanston Cultural Arts Programs and partially supported by the Illinois Arts Council, a state agency.
Interested in sponsorship opportunities for next year's festival?
Contact Patricia Battaglia, Festival Coordinator/Cultural Arts Programs, firstname.lastname@example.com or 847-448-8264.
2014 Sponsors
The Ethnic Arts Festival is supported by the Cherry Family Foundation.
Upcoming Events
Firewood Sale
ACA Navigators
Northwestern Football vs. Illinois
Holiday Bash and Tree Lighting
ACA Navigators
Recent News
- Nicor Construction in Dempster Street Area to Begin December 8
- Explanation for Recent Higher Energy Bills
- ComEd Work Scheduled in Alley North of Brummel St. Dec. 5
- Small Businesses Encourage You to "Shop Evanston" on November 29
- Attend the 2014 Evanston Holiday Safety Forum Dec. 2


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I have written often and often about the dangers a nation faces when its press is not free:It's so tiring, you almost don't want to bother saying it. But we have to keep pointing it out, this mad descent of the press, and not grow weary or complacent. The only way to keep the press honest is to keep it accountable; that is our responsibility - yours and mine - a free press is an unencumbered, detached, open and honest press. And we must have it; we must insist that our press be free - una … [Read more...]
Our Unfree Press – -UPDATED
Interesting facts & notions – UPDATED
In my email, today, One Baby, One Vote:49 Million American babies have been aborted since 1973; 25 million would have been old enough to vote in this election.Read on...I'm still on the mat but trying to get up. Check back through the day. I'll try to at least amuse...as with this bit of zydeco.And, oh, yeah, did you read about the new cabinet-level offices planned under Obama? I may go buy a gun when I feel better, just on principle. Orwell was only off by 24 years.Obama is fraud … [Read more...]
The stupidest girl in the world
Had a doctor's appointment so I missed this when it broke.I'm so glad I followed my gut and didn't buy into this story:How stupid and/or needy is this kid?Police sources tell KDKA that a campaign worker has now confessed to making up a story that a mugger attacked her and cut the letter "B" in her face after seeing her McCain bumper sticker.Ashley Todd, 20, of Texas, initially told police that she was robbed at an ATM in Bloomfield and that the suspect became enraged and started … [Read more...]
Joe the Plumber conquers the press?
Big family doings through Sunday and part of Monday will have things quiet around here. Be well!Hey, do you think Obama and Ayers ran into each other, once in a while?Meanwhile, the press cannot be happy with the reality (and the anger) they have helped to create:From Iowahawk, who is pulling no punches:Politicians -- Sarah Palin, Bill Clinton, et al. -- obviously have to put up with some rude, nasty shit, but it's right there in the jobs description. Joe the Plumber is different. He … [Read more...]
Live-blogging the final Debate?
No, not me. I haven't the stamina to watch-and-blog because I get too anal about trying to get "everything." Not worth making myself nuts over. While you're waiting though, take a look at what the Ohio Secretary of State thinks is Democracy in Action, and get a load of Gay Patriot's state-by-state electoral fraud post. It ain't pretty.I see the press already has their talking points from Lord Obama.If you're looking for liveblogging, it's usually fun times for that over at Althouse's … [Read more...]
Ohio: A wee Banana Republic?
Yes, an obvious exaggeration, but this Secretary of State, Jennifer Brunner, needs to lose her job, yesterday.Almost as if she anticipated a court ruling a few hours later, Ohio Secretary of State Jennifer Brunner promised yesterday to give county elections workers access to a list of possibly problematic voter registrations.She'd been sitting on them, you see. Something like 200,000 of them.She's been told to provide by Friday; says can't do it until Monday at least. Gotta buy … [Read more...]
ACORN, Media & the Angry Right
We're watching the press out-and-out lie in its reportage. We're watching YouTube censor what may negatively impact their man. Networks will sell 1/2 chunks of airtime to the Obama campaign, but will not run an ad by his critics. Ann Althouse notes that basic accurate transcription appears to be beyond the press' capability.No wonder the GOP rank-and-file are edgy, and angry. They know what "free speech" is. They've been gritting their teeth and respecting the first amendment for the last … [Read more...]


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A large crewneck used for robbing someone.
"Ay Blood That Nigga Ran Up On Me With A Goon Neck Real Talk"
by Stocktown's Finest November 05, 2011
10 Words Trending Now
Alphabetical List
Type your email address below to get our free Urban Word of the Day every morning!
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Tattoo Artist and Model Cleo Wattenström
Cleo Wattenström is truly a rare find—a tattoo artist who is known for both the beautiful art she creates and her striking looks. You're going to need to head to Sweden in order to check out her work but this… [ read more ]09/24/14
Tattooed Model Madzilla
The beautiful Madzilla has appeared in Inked Girls multiple times, but that didn't seem like any reason not to create a gallery of some of our favorite pictures of her. The gorgeous model has plenty of curves that drive us… [ read more ]09/16/14
A Hacker Stole More Than Celeb's Nudes
Inked is fucking disgusted by The Fappening NSFW Gallery follows the text The ice in the cooler had mostly melted into semi-chilled water and you were feeling around for the last summer brew while the biggest Internet event of the… [ read more ]09/02/14
Tattooed Model Shonda Mackey Part 2
The gorgeous Shonda Mackey has been the subject of one of our photo galleries in the past. From the minute it became live we were receiving requests to put up even more photos of the tattooed model. We heard you… [ read more ]09/02/14
Inked Digital Dream Girl: Christie Jay
Gallery follows the text. When did you first get tattooed and what's the story behind it? My first tattoo was when I was 16. My best friend and I decided we wanted each others lips on each other forever. We… [ read more ]08/28/14
Bettie Page Tattoos
There were pin up models before Bettie Page and there have been pin up models since Bettie Page but it is impossible to think of any other model when thinking of pin ups. Bettie Page was THE pin up model…. [ read more ]08/21/14
Friendship Tattoos
Getting inked together is one of the best bonding experiences people can have. We've already shared a list of tattooed couples with you but what about those people that get tattoos with their friends? If you really think your friendship… [ read more ]08/20/14


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Lehman Brothers
60 Minutes Revisits Lehman, Valukas, and Repo 105
By Ryan Chittum Apr 24, 2012 at 07:19 PM
Remember the Valukas Report? That court-appointed bankruptcy examiner's investigation into the collapse of Lehman Brothers found a number of colorable... More
Audit notes: Buffett on newspapers, Times-Picayune, SEC lets Lehman go
A vow to invest in newspapers and protect them from interference
By Ryan Chittum May 24, 2012 at 05:59 PM
This is the most hopeful thing I've read about the business of newspapers in a long, long time: I'll quote... More
Audit Notes: When a Loss Isn't a Loss, Lehman, WSJ on Nukes
By Ryan Chittum Mar 15, 2011 at 01:01 AM
You're going to hear a lot about all the "losses" insurers are going to be taking on the catastrophe in... More
Best of 2010: Ryan Chittum
Chittum picks his top stories from
2009 2010
By Ryan Chittum Dec 24, 2010 at 01:20 PM
Business Journalism on Prozac: A look at an issue of Fortune finds the magazine painting a picture of the corporate... More
Serial creators don't know what will happen to Adnan Syed - New developments in his legal case suggest that the outcome is wide open
The press is responsible for ignoring Bill Cosby rape allegations - Where were journalists 10 years ago when claims originally surfaced against him?
Journalism has a plagiarism problem. But it's not the one you'd expect - Fareed Zakaria's case highlights news organizations' ethical grey areas
4 topics John Oliver explained more clearly than television news - The political satirist brings explainer comedy to HBO viewers
Will radio save science journalism? - WNYC will soon have a new health unit
My take: The end of an era in Tampa (14)
Serial creators don't know what will happen to Adnan Syed (14)
The press is responsible for ignoring Bill Cosby rape allegations (6)
The Boston Globe finally digs in to a big story (4)
Journalism has a plagiarism problem. But it's not the one you'd expect (4)
Email blasts from CJR writers and editors
CNN reporters in Ferguson provide coverage of CNN reporters in Ferguson (Slate)
"[T]here was little that justified CNN's egocentric coverage"
Which media outlets use 'illegal immigrant'? (Fusion)
"[I]n spite of all the good reasons not to use the phrase, it is still very easy to find in the US press, even in headlines"
Jack Shafer on losing his job (Capital New York)
"Right now, my immediate plan is to go to work as a lay therapist at The Intercept to bring the healing there so John Cook and Matt Taibbi can return. I have great interpersonal skills."
On outlets that continue to call unknown drone victims 'militants' (The Intercept)
"Like the US drone program itself, this deceitful media practice continues unabated"
Greg Marx discusses democracy and news with Tom Rosenstiel of the American Press Institute
CJR's Guide to Online News Startups
ACEsTooHigh.com – Reporting on the science, education, and policy surrounding childhood trauma
Who Owns What
The Business of Digital Journalism
A report from the Columbia University Graduate School of Journalism
Questions and exercises for journalism students.
Should all journalists be on Twitter? – Reasons to take up or forgo the 140-character platform
Is it ever okay to name victims? – Editors question the policy of omitting information in sexual assault and domestic violence cases


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The White House Blog: The Vice President
Law Enforcement Officials Discuss Keeping Our Communities SafePosted byon January 28, 2013 at 3:02 PM EST
Today, President Obama and Vice President Biden continued the conversation about reducing gun violence in a meeting with law enforcement officials.
No group is more important in helping make our communities safer, President Obama said, because they recognize that it's not only the high-profile mass shootings that need to be addressed, but also the everyday gun violence that happens in cities and towns across the country.
That's why part of the conversation that we're going to be having today relates not only to the issue of new laws or better enforcement of our gun laws, it also means what are we doing to make sure that we've got the strongest possible law enforcement teams on the ground? What are we doing to hire more cops? What are we doing to make sure that they're getting the training that they need? What are we doing to make sure our sheriff's offices in rural counties have access to some of the resources that some of the big cities do in order to deal with some of these emergencies?
Two weeks ago, President Obama issued 23 executive actions to help reduce gun violence, and called on Congress to pass laws that will help keep guns out of the wrong hands, ban assault and high-capacity magazines, make our schools safer, and increase access to mental health services. Read more about the plan here.
West Wing Week 01/25/13 or: "Behind the Scenes: Inauguration 2013"Posted byon January 24, 2013 at 9:10 PM EST
This week, Washington D.C. was transformed into an historic stage, with an estimated one million people on hand to witness the President and Vice President take the Oath of Office at the United States Capitol. Through it all, West Wing Week was there, from the terrace of the Capitol to backstage at the balls. America, take a moment to look back at where we've been this week for this special edition of West Wing Week, January 18th to the 24th, or "Behind the Scenes: Inauguration 2013."
Watch: "Fireside Hangout" with Vice President Biden on Reducing Gun ViolencePosted byon January 24, 2013 at 5:20 PM EST
This afternoon, Vice President Biden sat down with Americans from around the country to talk about proposals to reduce gun violence in a live Google+ Hangout. During the virtual roundtable, participants from all different backgrounds asked the Vice President about topics ranging from an assault weapons ban to the Second Amendment and from mental health checks to making our schools safer. In case you missed the event live, check out the full video below, and learn more about the plan.
This "Fireside Hangout" was hosted by Google and moderated by Hari Sreenivasan of PBS NewsHour with guests including: Guy Kawasaki, an author and technology expert; Phil DeFranco, a media entrepreneur and host of the Phil Defranco show on YouTube; Theresa Ttillett, a mother and grandmother living in Hartford, CT; and Kimberley Blaine, a blogger and therapist who leads several parenting communities on Google+. This was the Vice President's first hangout, and the first White House hangout of the second term.
Fireside Hangouts: Vice President Biden Joins a Conversation on Reducing Gun ViolencePosted byon January 23, 2013 at 12:45 PM EST
Ed. Note: The hangout with Vice President Biden has concluded. You can watch the full video here.
This week, the White House will continue a series of conversations with top administration officials on Google+. On Thursday, January 24 at 1:45 p.m. ET, Vice President Biden will join the latest "Fireside Hangout" – a 21st century take on FDR's famous radio addresses – to talk about reducing gun violence.
On January 16, President Obama and Vice President Biden released a plan to help protect our children and our communities by reducing gun violence. The plan outlines specific, common-sense steps we can take right now to help keep guns out of the wrong hands, ban assault weapons and high-capacity magazines, make our schools safer, and increase access to mental health services. Learn more about the plan here.
During the hangout hosted by Google and moderated by Hari Sreenivasan from PBS NewsHour, Vice President Biden will discuss the White House policy recommendations on reducing gun violence. Google+ users from the around the country will join the discussion, including Guy Kawasaki and Phil DeFranco. If you have a question, you can suggest it by writing it on the participants' on Google+ pages. Watch the hangout with Vice President Biden live on WhiteHouse.gov, or tune in to the White House's Google+ page or YouTube channel.
We'll continue to host hangouts with key members of the President's cabinet on a range of second term priorities. Follow us on Google+ for updates from the Administration and opportunities to participate in upcoming hangouts.
President Obama and Vice President Biden Take the Oath of OfficePosted byon January 20, 2013 at 1:00 PM EST
Today, in two separate, private ceremonies, President Obama and Vice President Biden were officially sworn into office, marking the start of the second term. (The Constitution mandates that the President takes the oath on January 20. Since that date falls on a Sunday this year, the public inauguration ceremony and festivities will take place tomorrow, January 21)
President Obama took the oath, administered by Chief Justice John Roberts, in the Blue Room of the White House, using a bible that belonged to First Lady Michelle Obama's paternal grandparents.
Vice President Biden was sworn in by Justice Sonia Sotomayor in front of family and friends in a ceremony at the Naval Observatory. Vice President Biden took the oath using the Biden family bible.
Giving Back on the National Day of ServicePosted byon January 19, 2013 at 5:30 PM EST
Today, the First Family kicked off Inauguration weekend by participating in the National Day of Service, helping out with some school improvement projects at Burrville Elementary in Washington, DC.
President Obama asked Americans around the country to take part in the National Day of Service to honor the life and legacy of Dr. Martin Luther King, Jr., whose birthday falls on Inauguration Day this year. The President and First Lady asked that we all remember the importance of giving back and looking out for others – both central to Dr. King's work – as we celebrate this weekend.
"This is really what America is about," President Obama said. "This is what we celebrate." He said that this Inauguration is "a symbol of how our democracy works and how we peacefully transfer power, but it should also be an affirmation that we're all in this together and that we've got to look out for each other and work hard on behalf of each other."
West Wing Week: 01/18/13 or "#NowIsTheTime"Posted byon January 18, 2013 at 12:00 AM EST
This week, President Hamid Karzai came to the White House, as did nine newly posted foreign ambassadors and the President held the final news conference of his first term before signing executive orders initiating 23 separate executive actions to prevent gun violence.
President Obama Announces New Measures to Prevent Gun ViolencePosted byon January 16, 2013 at 1:57 PM EST
President Obama today announced a series of sweeping reforms that will help curb gun violence in our nation.
In front of a crowd that included victims of gun violence, families who lost loved ones to gun violence, elected officials, and school children who had written letters asking him to do something to prevent more senseless massacres like the one at Sandy Hook Elementary School, the President introduced a comprehensive proposal that will make it easier to keep guns out of the hands of criminals and will give law enforcement, schools, mental health professionals, and the public health community the tools they need to help reduce gun violence, and keep our children safe.
"This is our first task as a society," the President said. "This is how we will be judged. And their voices should compel us to change."
These actions are the result of the effort led by Vice President Joe Biden and members of the Cabinet to come up with concrete steps that we can take right now to keep our children safe, help prevent mass shootings, and reduce the broader epidemic of gun violence in this country.
The President acknowledged that implementing some of these changes will be difficult, but vowed to make it a priority: "I intend to use whatever weight this office holds to make them a reality. Because while there is no law or set of laws that can prevent every senseless act of violence completely, if there is even one thing we can do to reduce this violence – if even one life can be saved – we have an obligation to try."
West Wing Week: 01/11/13 or "The Interests of Our Country"Posted byon January 11, 2013 at 12:00 AM EST
Welcome to the West Wing Week, your guide to everything that's happening at 1600 Pennsylvania Ave. This week, the President nominated a bipartisan slate of leaders to key administration jobs and the Vice President met with a wide array of organizations to talk about efforts to reduce gun violence.
Vice President Biden Meets with Groups to Discuss Violence PreventionPosted byon January 10, 2013 at 10:16 AM EST
Right now, Vice President Biden is leading an effort to develop a new set of policies to reduce gun violence and prevent tragedies like the shooting in Newtown Conn.
Yesterday, as part of that work, the Vice President met with victims' groups and gun safety organizations at the White House.
Following those meetings, the Vice President held conference calls with governors of both parties, and state and local elected leaders from across the United States. The Vice President listened to the unique perspectives of all the participants, and solicited their ideas and input on how to curb gun violence in this country. During the call, Mr. Biden reiterated the Administration's commitment to this urgent issue and stressed that the problem requires immediate action.
Today, Vice President Biden will hold meetings with advocates for sportsmen and women and gun ownership groups. He will meet with representatives of the entertainment and video game industries this week as well.
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What does BCID stand for?
What does BCID mean? This page is about the various possible meanings of the acronym, abbreviation, shorthand or slang term: BCID.
We've found a total of 4 definitions for BCID:
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BookCrossing IDentification
Battlefield Combat Identification
Brooklyn Center for Independence of the Disabled
Black Canyon Irrigation District
Still can't find the acronym definition you were looking for? Use our Power Search technology to look for more unique definitions from across the web!
"BCID." Abbreviations.com. STANDS4 LLC, 2014. Web. 23 Nov. 2014. <http://www.abbreviations.com/BCID>.


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Posts Tagged «geomagnetic storm»
Earth's magnetic field could flip within our lifetime – but don't worry, we should be OK October 21, 2014 at 3:08 pm
This summer, the European Space Agency published data that suggested that the Earth's magnetic field could flip — as in, the magnetic north pole becomes the magnetic south pole — in 'a few thousand years.' At the time, I figured there was no rush to write it up — after all, we might not even be living on Earth in a few thousand years. Now, however, new research published this week shows that the magnetic field might flip within our lifetime — so it's probably something that we ought to talk about.
The solar storm of 2012 that almost sent us back to a post-apocalyptic Stone Age July 24, 2014 at 8:25 am
While you didn't see it, feel it, or even read about it in the newspapers, Earth was almost knocked back to the Stone Age on July 23, 2012. It wasn't some crazed dictator with his finger on the thermonuclear button or a giant asteroid that came close to wiping out civilization as we know it, though — no, what nearly ended us was a massive solar storm. 'If it had hit, we would still be picking up the pieces,' says Daniel Baker, who led the research into the massive solar storm.
Space invasion: Solar storms pose critical threat to US infrastructure July 15, 2013 at 11:34 am
A new report on vulnerabilities in the US power grid sheds stark light on our ability to deal with certain kinds of threats. A coronal mass ejection of an equivalent magnitude to the one that hit the Earth in 1859 would cripple our infrastructure today, with some areas losing power for up to two years.


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I am very pleased to offer this 1988 Ferrari 328 GTB. This particular 328 GTB is an extremely rare color combination of Argento (silver)... More
This 1988 Ferrari Testarossa is an beautiful original car and a superb driver. Red with tan interior. Only 22K original miles from new. A... More
Serviced With Major Service Performed On October, 2014. This 1988 Ferrari 328 GTS, Finished White With Red Leather Interior. One Owner, Driven Only... More
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1988 BMW 635CSi1988 BMW 635CSi model in mint conditionChampagne metallic exterior plus a two-tone Light-Beige and Black leather interiorEquipped with a V8 gas and... More
-Two California owners from new. -73,046 Original Miles -Clean Car Fax – No Accidents -Original Books -Extra Sets of Keys -Fresh California Smog Certification -Service Records From New to Present -Major... More
1988 Pontiac Firebird Trans Am1988 Pontiac Firebird Trans Am model in mint conditionGreen metallic exterior plus a Tan cloth interiorClassified as a 2-Door Coupe... More
1988 Porsche 911 Turbo Slantnose VIN: WP0JB0931JS050407 Info Coming Soon. -... More
GR Auto Gallery is pleased to present this 1988 Chevrolet Suburban R20 Silverado. The Suburban is the longest continual nameplate in production in the... More
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F40 Motorsports is proud to offer this gorgeous two owner 1988 Porsche 911 Carrera Cabriolet. This 911 has only 45,900 pampered miles and is... More
1988 FORD MUSTANG GT 5.0 V8 CONVERTIBLE BEAUTIFUL RED PAINTWORK VERY NICE RED INTERIOR VERY CLASSY LOOKING CONVERTIBLE IN VERY GOOD CONDITION IN CALIFORNIA... More
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Chequered Flag International is pleased to offer this 1988 Porsche 930 Cabriolet in Grand Prix White with Burgundy interior & Black soft top. 72,520... More
1988 Cadillac Fleetwood stretch limousine, 1 owner, low mileage, excellent original condition, $8,500.... More
Two-owner, superbly-kept 1988 Mercedes-Benz 560SL. Signal Red / Palomino, leather upholstery, AM/FM radio w/ CD Player, removable hardtop. More info coming soon.... More
This 1988 Ferrari 328 GTS is in Rosso Corsa with Tan and has less than 28k miles. The car has been in Arizona since 2000.... More
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1988 CHEVROLET CORVETTE CHALLENGE CAR ID#1G1YY2187J5113471 This ex-Mike Yeager car is 1 of only 50 Corvette Challenge Race Prepared Cars made in 1988. Each car was... More
1988 Chevrolet Corvette Callaway Convertible, This is a clean title, actual mileage Callaway Corvette. It is one of 16 built in an automatic. We... More