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Birds don't usually fly into mirrors unless they feel agressive, eg mating season. As they approach the mirror they see themselves and think it is another bird. It's not like windows when the glass is clear.
Snap for hydrangea, Fairygirl. Petiolaris is the only one that likes it here anyway.
Super looking cakes.
My Mother-in-law gave me some London's Pride when we moved into our first house in 1973. I took some with me when we moved to Kent in 1978. Then we moved to France in 1985 and to our 2nd house in France in 1990 and then my Sister-in-law gave me some which I still have in 2 places. She said I had given it to her. Now you've reminded me I think I'll put some in the shady end of my new bed.
Honeysuckle, an essential I think. I have several and their scent fills the air when they are out.
I would spray it all with glyphosate. When it's dead it's not hard to rake it all up. Then rotavate. I know it'll take longer but it's worth it. Our lawn used to be an old farmyard that a previous owner had added earth to. When we bought our house it had been empty for 6 years. The ?lawn was full of nettles brambles and weeds. We hired professionals to deal with it as we were busy doing the house. They just mowed it all then rotavated, then sowed grass and I regretted it for years afterwards. Especially as it had bindweed as well which got into the beds.
Hello Alan. I tried once. One dried properly, the others went mouldy! Are you using a pad or a laptop? I don't think smileys work on a pad.
You can crop strawberries in their first year, MMP, but remove the flowers for the first few weeks after planting out to help the roots get established and improve the plant strength. I have Toscana as well which are to go in a pot, but I put violas in it, so they will have to wait.
Morning all, looks like another lovely day. First I'm off to the GH to repot the tomatoes that are getting too big for their pots. Then planting in new bed then weeding. Most days will be weeding for a bit! We could do with a spot of rain here too, preferably in the night!
If we are still on G there is Gunnera. We saw lots along the streams in Cornwall in Trebah and the Lost Garden of Heligan. Magnificent, but probably too big for most of us!
I think a wild flower meadow mix would be good too. They can look lovely. Here is a pic of one we saw in Yorkshire last summer.


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You can pre-order "Movin' On Up: The Music and Message of Curtis Mayfield & The Impressions" at Amazon.com
A great song!!
Posted by:
Ferrus |
April 18, 2008 at 03:23 AM
Cool! I would love to see the movie as I used to listen to his music with my father and I think he would love to see him again too if only in a movie
Posted by:
Judy |
April 18, 2008 at 07:42 AM
What a genius. Thanks for posting this. His voice was so amazing.
Posted by:
Miss Lisa |
April 19, 2008 at 12:21 AM
Thanks for this sheet. Very crazzy film.
Posted by:
indir |
July 29, 2008 at 09:48 AM
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Spill the beans: Revenue will stay solid, but competition from foreign producers threatens growth
IBISWorld's Soybean Farming market research report can be used to help you: understand market size and growth potential; major companies; draft business plans and pitch books; and conduct benchmarking and SWOT analysis. Our industry analysis highlights macro industry trends in the overall sector and micro trends faced by companies that do business in the industry. The industry report also provides key industry statistics and 5-year forecasts to anticipate future industry prospects so you can decide with confidence.
Although US soybean farmers have grown in the past five years, the industry will continue to face pressure from international operators. With Brazil expected to overtake the United States as the top provider of soybeans, domestic operators will continue to fight for profitability and market share, while also battling volatile planting conditions. Additionally, as more Americans steer clear of processed foods, the industry will rely on demand from sectors like alternative fuels... purchase to read more
The Soybean Farming industry is overwhelmingly characterized by family-held individual farms. In most cases, soybean producers operate as partnerships and sole proprietors rather than corporations. Despite some farm consolidations, the industry remains fragmented. According to the US Department of Agriculture, the average soybean farm generates about $67,333 in soybean sales a year.
There is a long-term trend toward fewer farms across the agricultural sector, and this applies to the Soybean Farming industry as well. This trend is a result of rising costs and competition that make it difficult for smaller farms to succeed. Owners of these small farms are forced to form cooperatives or sell their land... purchase to read more
Farms in this industry grow soybeans as their main crop. Soybeans are most often used in livestock feeds and vegetable oils, with a small but growing proportion being used in biofuel production. Establishments that sell soybean seeds to US farmers for growing crops are also included.
Industry Definition
Main Activities
Similar Industries
Additional Resources
Executive Summary
Key External Drivers
Current Performance
Industry Outlook
Industry Life Cycle
Supply Chain
Products & Services
Major Markets
Business Locations
Competitive Landscape
Market Share Concentration
Key Success Factors
Cost Structure Benchmarks
Barriers to Entry
Capital Intensity
Industry Data
Annual Change
Key Ratios


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FREILASSING, GERMANY — Br�ckner Group subsidiary Kiefel GmbH is providing machines and tools to produce doors for the new Mercedes C-Class in a deal worth several million dollars.
This in-depth report examines the Brazilian plastics industry from a historical and geographical context. Our analysts provide insight on economic trends and forecasts, growing manufacturing sectors that utilize plastics, private investment opportunities, market environment challenges, and innovations in R&D.
Data tables and charts on producer prices, trade, plastics production and end market indicators is also included.
This report is a review and analysis of the North American Plastics Recycling Industry, including key trends and statistics based on 2013 performance. We examine market environment factors, regulatory issues, industry challenges, key drivers and emerging trends in post-consumer and post-industrial recycling.Learn more
This special package contains our 132-page 2014 Market Report on the Injection Molding segment and our exclusive 2014 RANKINGS database of 500+ Injection Molders for a discounted package price.Learn more
January 14, 2015 - January 14, 2015Plastics in Automotive
February 4, 2015 - February 6, 2015Plastics News Executive Forum 2015
June 2, 2015 - June 3, 2015Plastics Financial Summit - Chicago 2015
September 16, 2015 - September 18, 2015Plastics Caps & Closures - September 2015
October 27, 2015 - October 29, 2015Plastics Financial Summit - New York - 2015More Events


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This 1979 International Scout II is fresh from a no-expense-spared restoration. Clad in Emerald Green Poly with a matte black Rallye stripe kit, paint... More
6cyl 4 spd - no Rust, manual 2 speed, rear axle - restored to drive, very nice truck for the money. Call Harold 1-315-439-6348... More
Retired Correctional Facility Fire Truck. Third Owner. Vehical has less that 7,000 miles and is fantastic mechanical condition. 8' stepside bed was removed and... More
Custom Stage coach a truly one of a kind vehicle only a handful of these were made.Built on an International Scout chasis with a... More
Westchester sub body, 350 V8, 700 R4, cold a/c, ps, pb, 9" rear mustang II sups. chrome illusion paint, nice, drive anywhere... More
WE are parting several 40s-50s Internationals. 800-447-2886, OK; www.eastwestautoparts.com**14-YR**... More


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Doctor Who: Podshock will be live this Sunday, November 30, 2014 at 4pm EST. The crew will review the entire season of Doctor Who.read more
HR to Who: From Human Resources to Doctor Who
I chose to combine my professional career, Human Resources, and one of my favorite programs, Doctor Who, when creating this blog. This is the home for my ideas and serves as my creative outlet. I've broken away from the mold of a single-topic blog and may write about anything from current events, comic books, social media, or the topics mentioned above. The name and logo for this blog was carefully chosen as was the tagline. HR to WHO universally covers the scope of any information I might choose to cover - social media, comic books, sci-fi, performance, work, television, news, and much more. So, thanks for reading.
Please comment and join the HR to WHO discussion.
This site provides information on a variety of topics including: social media, human resources, blogging, personal commentary, and Doctor Who.
I am an official contributor to the Performance I Create blog as of October 2014.
Performance I Create is a contributor designed blog for professionals to share their knowledge and experience about improving performance and productivity through human performance improvement, training/learning and development, process improvement, instructional design, human resources, communication, social media, leadership, or productivity.
Contributors include trainers, HR professionals, managers, employees, professors, researchers, and anyone using forward thinking to impact performance and productivity.
Doctor Who is television's longest running science fiction program. It began on November 23, 1963 with "An Unearthly Child" and continued the original run until "Survival" in 1989. The BBC, in a joint venture with Fox, commissioned and created a made-for-television movie in 1996. While the movie performed well in the United Kingdom, it did not generate enough ratings in the United States to warrant a full series. Fans of Doctor Who did not see The Doctor return until 2005 with the episode "Rose." This episode began what has become not only a revival but a regeneration of the Doctor Who franchise for the 21st century.


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A boggy chop dill munch with a cheesy tank wank.
Yee Droondizzle is too close to my nose.
by zroach June 18, 2009
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Seven fires within a month have caused $25,000 in damage to equipment and facilities around the amphitheater, officials said.
Graphic: Fires grow in scale, jangle nerves at Fullerton's Muckenthaler center
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Brand ResearchPorsche
Archived Instrumented Test1994 Porsche 911 Turbo 3.6
The people's Turbo.
Comparison Tests1991 Chevrolet Corvette ZR-1 vs. 1991 Porsche 911 Turbo
Predators: The horsepower wars continue.
Archived Instrumented Test1991 Porsche 911 Carrera 2 Tiptronic
Look, mom, somebody put mud flaps on my Tomcat!
Archived ComparisonPorsche 911 Carrera 4 Cabriolet vs. Acura NSX, Chevrolet Corvette ZR-1, Ferrari 348ts, Lotus Esprit Turbo SE
The Eroticars: Lust at first site.
Archived Instrumented Test1990 Porsche 911 Carrera 2
Enthusiasts, rejoice: the "2" means twice the fun.
Archived Instrumented Test1990 Porsche 911 Carrera 4
Germany's new low-flying four-wheel-drive bahn bomber.
Archived Instrumented TestPorsche 959
Twenty-four hours in sports-car heaven.
Archived Instrumented Test1987 Porsche 911 Turbo Cabriolet
Your wish is the Zuffenhausen factory's command.
10Best Cars1986 10Best Cars
Trust us. They dont get any better than this.
Archived Instrumented Test1986 Porsche 911 Turbo
The vexing return of the car no one could forget.
10Best Cars1985 10Best Cars
This isn't getting any easier.
Archived ComparisonAudi Quattro vs. Ferrari 308 Quattrovalvole, Honda Prelude, Lotus Esprit Turbo, Porsche 911 Carrera, 928S, and 944, Toyota Celica Supra
The best-handling imported car is...
Archived Instrumented Test1984 Porsche 911 Carrera
Germany's holy roller, the fastest factory-fresh, finger-in-the-socket, load-and-cock it full-time pocket rocket. Amen.
10Best Cars1984 10Best Cars
It would have been much easier to pick the twenty best from 1984's bumper crop.
Archived Instrumented Test1983 Porsche 911SC Cabriolet
Porsche's three-digit widget goes alfresco.
10Best Cars1983 10Best Cars
Some are born great, some achieve greatness, and some have greatness thrust upon them, but the Zastava Yugo still failed to make the cut.
Archived ComparisonRed Speed: Chevrolet Camaro Z28 vs. Ford Mustang GT, Pontiac Firebird Trans Am, Porsche 928
Gauging the good, better, best of the all-American road machines against the ultimate standard.
Comparison TestsDe Lorean vs. Chevy Corvette, Datsun 280-ZX, Ferrari 308GTS, Porsche 911
De Lorean Versus the World: Four on one in a three-round GT title bout.
Comparison TestsAlfa Romeo Spider vs. Chevy Corvette, Datsun 280-ZX, Fiat Spider 2000, Mazda RX-7, Porsche 924
Just For Fun: The times they are a-changin', sports-car wise.
Archived Instrumented Test1978 Porsche 911 SC
It may be the last 911. It may also be the best.
Archived Instrumented Test1977 Porsche 911S
For people who like their pleasure in soft packages.
Archived Comparison1974 Porsche 911 vs. 911S Targa, 911S Carrera
Comparing the 911, 911S Targa, and 911S Carrera.
Archived Comparison1972 Porsche 911 T Coupe vs. 911 E Targa, 911 S Coupe
The "T", "E", and "S" all have a new 2.4-liter engine but differences are vast.
Archived Instrumented Test1963 Porsche 356B 1600 Super
Not everyone's cup of tea, the Porsche is still one of the most "in" cars a man can drive.
Archived Instrumented Test1968 Porsche 911 Sportomatic
Like the car itself, Porsche's "automatic" transmission takes a bit of getting used to.


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Little Bird Told Me is an imaginative brand that's our go-to for rocking toys and activity mats for curious tots. Built to last a lifetime, these developmental activity toys, innovative infant rocking animals & horses and nursery accessories are all wrapped up in beautiful eco-friendly gift style packaging -- another gift to the future.
Comfy PJs, swaddle wraps, and more after-hours finds for parents-on-duty.
All of the bright, whimsical pieces you need to make their space totally unique.
Serious deals on the cutest clothes, shoes, gear, and more.
Colorful block sets, wooden animal puzzles, and more for hours of serious fun.


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You're leaving Wedding Paper Divas to see the Deal of the Day at our parent site, Tiny Prints.
You won't be able to dismiss this by usual means (escape or click button), but you can close it programatically based on user choices or actions.
"Part of the beauty was in the card itself. Great design, carefully and lovingly customized in my wedding colors. Kudos to my two designers! If I had to do it over again, I would've done everything the same. Paper frosting, fonts chosen, quality, etc. People are going to love these."
"These invitations turned out great! Loved the contrast with light colors on the front and the black on the back. Wedding paper divas was very responsive to all of my changes, and got things done quickly. Would definitely recommend!"
"I loved this card even more in person, the paper, the texture and it just has a great balance. We are going with this one in black."
"I ordered a sample of this invitation in blue, and it's beautiful, but the black really makes it seem like night. My ceremony is in the afternoon, so it didn't seem to fit, but it really is a nice card and I would have chosen if not for all the black."
"With only a few weeks to plan my wedding, I was so excited to find an on-line source that was easy to use in creating our wedding invitations. The design options were numerous (almost overwhelming!), but they give you lots of ways to narrow down your search for the perfect invitation. I was able to design and save several options, show them to my fiance and we made a very quick decision. Way easier than the old way of doing this (pre-internet)! The invitation arrived very close to the estimated delivery date...only one day late, but that's because I did a major rush order. I feel that Wedding Paper Divas did a fantastic job meeting my timelines and provided me with excellent customer service during the process. Thanks!!"


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Majority of Republicans Back Workplace Protections
August 1, 2013 by Maureen McCarty, HRC Associate Director of Digital Media
Americans from all walks of life share the basic value that everyone should have the opportunity to get a job and provide for themselves and their families. In fact, an overwhelming 70 percent of Republicans support LGBT workplace protections.
Despite this broad and bipartisan support, there is currently no federal law that bans workplace discrimination based on sexual orientation or gender identity. Now, Congress has the opportunity to codify this shared value into federal law.
It is time for nationwide workplace protections for LGBT Americans. To show your support and to help HRC to spread the word about ENDA, share the image below now.
And to learn more about the broad, bipartisan coalition working together in support of ENDA, visit www.workplaceopportunity.org
Top Posts
Marriage Equality Coming to Arizona
October 17, 2014
Federal Judge Blocks Kansas's Ban on Marriage Equality
November 4, 2014
Shameful Sixth Circuit Decision Upholds Discriminatory Marriage Bans
November 6, 2014
Seismic Shift in Rome: New Catholic Church Document Praises Committed Gay and Lesbian "Partnerships"
October 13, 2014
Marriage Equality Coming to Wyoming
October 17, 2014


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Duck Dynasty Season 7 Premieres Tonight on A&E
"Glory Is The Reward of Mallard." That's the title of tonight's season 7 premiere for our beloved Duck Dynasty on A&E!
"Glory Is The Reward of Mallard." That's the title of tonight's season 7 premiere for our beloved Duck Dynasty on A&E!
Duck Dynasty Daughter Sadie Robertson is into the Semifinals on ABC's Dancing With the Stars. On Monday, the Louisiana teen earned a perfect "10" score from DWTS alum Julianne Hough for her quickstep with partner Mark Ballas.
Duck Dynasty daughter Sadie Robertson is likely to advance to the next week of ABC's Dancing with the Stars after she wow-ed the judges again. This time with a "scary" zombie inspired dance routine with partner Mark Ballas
Duck Dynasty star Sadie Robertson continues to do well on ABC's Dancing With the Stars. Monday night, she showed a new side of her dancing skills with a sexy rumba.
Sadie Robertson of the Duck Dynasty family sailed through week four of Dancing with the Stars with a scintillating samba with partner Mark Ballas.
Season 6 of the A&E reality hit premiered Wednesday night (June 11) , drawing significantly fewer viewers than the Season 5 premiere in January.
A&E's popular show "Duck Dynasty" begins its sixth season tonight (June 11), and a special guest is paying The Robertson family a visit: Louisiana Governor Bobby Jindal.
Independence Bowl officials have confirmed that Duck Commander is now the title sponsor.
It's a tight race between Democrat Senator Mary Landrieu and Republican Congresman Bill Cassidy. But a new poll shows that if Phil Robertson ran against Landrieu, he'd be the favorite.
West Monroe's Duck Commander is taking its name to NASCAR.
There were a number of stars on hand last night in Washington DC for the Presidents State of the Union Address. Louisiana natives Willie and Korie Robertson were by far the most popular. The Duck Dynasty CEO was the special guest of Louisiana Rep. Vance McAllister who extended the invite as a thank you for throwing his support behind McAllister last year, helping to propel the congressman to victory in a special runoff election.
After the recent controversy with Phil Robertson that had 'Duck Dynasty' viewers quacking on every side, the show returned to A&E for its fifth season Wednesday (Jan. 15) and was met with less than stellar ratings.


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Michael Jackson death anniversary: King of Pop remains as relevant as ever
It's been five years since Michael Jackson died, yet his career is more alive than it has been in decades.
Michael Jackson's 'Thriller' anniversary: Album released 30 years ago FridayComment
It was 30 years ago Friday that Michael Jackson's landmark album, Thriller, was released. Three decades later, nearly every song on the hit record still gets people moving and shaking.
RecommendedRecent Facebook Activity
Only On 7
The ABC7 I-team investigates cop cruiser crashesCop Cruiser Crashes happen more often than you think in the Washington area. Are officers being held accountable? And should you be paying the price? The I-Team investigates TONIGHT on ABC7 News at 11 after "Castle."


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Former APS Supt. Beverly Hall Bedridden; Will Not Be Attending Trial
Sources have confirmed former Atlanta Public School superintendent Beverly Hall will not be able to attend her trial which is set for August 11 due to her being bedridden from fighting stage 4 breast cancer. […]
Sheriff: Intoxicated Woman Rode Stolen Horse To Failed Shoplifting Attempt
An Alabama woman drunkenly rode a horse to a convenience store, where authorities say she attempted to shoplift items and then ride off on the beer-saddled animal.
Thieves Make Clean Getaway From CVS With Thousands In Hygiene Products
Three men made a clean getaway after stealing $2,000 in soaps, tooth pastes and other personal hygiene items from a Sandy Springs CVS store.
A Triple Shooting In Lithonia
(AP/WAOK) — Authorities say a teenager was killed and two other people wounded by gunfire in what police believe was an attempted robbery in suburban Atlanta. CBS 46 in Atlanta reports the shooting happened early […]
Farewell Breakfast For Bou Bou And Family
A farewell breakfast for the Phonesavanh family and their son, Baby Bou Bou, will be held on Wednesday, July 2 at the Delightful Eatz Restaurant as they prepare to return to their home in Wisconsin. […]
Too Much Truth Exclusive: Justice For Baby Bou Bou
This is a News and Talk 1380 WAOK exclusive, heard first here on "Too Much Truth" with Derrick Boazman. Prayers and support are still needed for 20 month old Bounkham Phonesavanh, also known as "Baby […]
Update On Baby Bou Bou Injured In Swat Raid
Atlanta (WAOK)- Nineteen month old Bounkham Phonesavanh who was critically injured in May when a flash grenade exploded in his play pen has been transferred to Children's Healthcare of Atlanta at Scottish Rite to begin rehabilitation. […]
Autopsy: Hyperthermia Killed Child Left In Hot SUV
A medical examiner in Georgia says hyperthermia was the cause of death for a toddler left for hours in his father's SUV.
4 Missing Riverdale Children Found Safe
Four children from Riverdale that were reported missing have been found safe. Anthony Delaney, 6, Zymeer Delaney, 4, Jeramiah Leavell, 3, and Osiyrus Leavell, 2, had last been seen around 10:30 Saturday morning at their […]
Unattended Child Rescued From Car In Augusta
A person who heard the infant crying called police, who rescued the child and arrested the mother.


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Hydrocarbon Engineering LNG Industry
Oilfield Technology
World Coal
World Pipelines World Cement
Palladian Publications.
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Total petroleum deliveries (a measure of demand) rose in June compared with May and with June a year ago and were up 1.9% for the first six months f 2011 over the same period in 2010. Gasoline demand was nearly flat compared with June a year ago, but up from both April 2011 and May 2011. Ultra low sulfur diesel demand continued to register strong numbers over 2010, achieving a record high.
'The growth in overall demand is consistent with the nation's modest but steady economic expansion,' said API chief economist John Felmy. 'Gasoline demand essentially treaded water while demand for ultra low sulfur diesel continued its year over year increase. Gasoline demand appears to still be held back by high unemployment and consumer uncertainty. On the other hand, the robust ultra low sulfur diesel demand numbers suggest US manufacturing is picking up the pace.'
US refinery gasoline production was at a record high for the month and for the year. For the month of June, distillate production was at a three year high while jet fuel production was at a six year high. Strong refinery production has reduced the need for petroleum product imports, which declined more than 30% in June compared with June a year ago. Crude imports declined 4.8%, and total imports fell 10%.
Crude oil production fell to a two year low and showed year over year declines for the second month in a row. At 5.4 million bpd, crude oil production was down by 2% from June 2010. For the second quarter, crude production was down by 0.1%. The preliminary reported data to API on offshore oil wells drilled show an 82% decline in the second quarter of this year compared with last. Despite the offshore drilling decline, API estimates onshore oil well completions, which include wells drilled on private as well as public lands, were at the highest second quarter level since 1985.
Crude stocks (excluding SPR) fell by 2.9% from the prior month and 1.5% from the prior year, but were still at the second highest stock level for any June since 1990, not counting 2010. With the exception of jet fuel stocks and residual fuel stocks, all key refined products' stock levels showed declines in June compared with May.
Published on 25/07/2011
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Robust Statistical Methods with RTaylor and Francis 2005; US$ 119.95
Robust statistical methods were developed to supplement the classical procedures when the data violate classical assumptions. They are ideally suited to applied research across a broad spectrum of study, yet most books on the subject are narrowly focused, overly theoretical, or simply outdated. Robust Statistical Methods with R provides a systematic... more...
Subjective Probability Models for LifetimesTaylor and Francis 2001; US$ 125.95
Bayesian methods in reliability cannot be fully utilized and understood without full comprehension of the essential differences that exist between frequentist probability and subjective probability. Switching from the frequentist to the subjective approach requires that some fundamental concepts be rethought and suitably redefined. Subjective Probability... more...
The Basics of S-PLUSSpringer-Verlag New York Inc 2006; US$ 169.00
Teaches the basics of S-PLUS Version 7.0 for people with little computing or statistical knowledge. This book demonstrates the important concepts of S-PLUS and R, its companion in implementing the S language. It also offers a guided tour of interactive computing, data exploration, and statistical analysis. more...
Advances In Statistical Modeling And InferenceWorld Scientific Publishing Company 2007; US$ 391.00
There have been major developments in the field of statistics over the last quarter century, spurred by the rapid advances in computing and data-measurement technologies. These developments have revolutionized the field and have greatly influenced research directions in theory and methodology. Increased computing power has spawned entirely new areas... more...
Probabilità Statistica e SimulazioneSpringer 2006; US$ 29.95
Il volume contiene in forma compatta il programma svolto negli insegnamenti introduttivi di statistica e tratta alcuni argomenti indispensabili per l'attivit di ricerca, come ad esempio i metodi di simulazione Monte Carlo, le procedure di minimizzazione e le tecniche di analisi dei dati di laboratorio. Gli argomenti vengono sviluppati partendo... more...
Inference for Change Point and Post Change Means After a Cusum TestSpringer 2007; US$ 169.00
Studies the bias of estimators and construction of corrected confidence intervals for change-point and post-change parameters after a change is detected by using a CUSUM procedure. This monograph includes case studies based on data sets from river flow, accident interval, stock prices, and global warming. more...
The Frailty ModelSpringer 2007; US$ 149.00
Discusses the statistical analysis of clustered survival data. This book presents different methods based on the frailty model and demonstrates how they can be used to analyse clustered survival data. more...
Robust Estimation and Hypothesis TestingNew Age International Pvt. Ltd., Publishers 2004; US$ 35.00
In statistical theory and practice, a certain distribution is usually assumed and then optimal solutions sought. Since deviations from an assumed distribution are very common, one cannot feel comfortable with assuming a particular distribution and believing it to be exactly correct. That brings the robustness issue in focus. In this book, we have given... more...
Data Manipulation with RSpringer 2008; US$ 79.95
Everyone using R needs to work with data, data almost always comes from an external source that has to be imported into R. The tasks covered in this book are essential tasks in R. more...
Statistics of ExtremesWiley 2006; US$ 146.00
Research in the statistical analysis of extreme values has flourished over the past decade: new probability models, inference and data analysis techniques have been introduced; and new application areas have been explored. Statistics of Extremes comprehensively covers a wide range of models and application areas, including risk and insurance: a major... more...


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Resources and Publications
Resource and Publication
Policymakers should take measures to reduce spam and improve cybersecurity on the Internet.
ITIF proposes a solution to consistently fund next-generation clean energy breakthroughs.
ITIF, Heritage, and CAP propose a set of nonpartisan policy proposals for reforming the National Laboratories.
ITIF's choices for top tech and innovation books of 2013.
All but "final mile" mail delivery should be opened up to private competition.
ITIF presents a rebuttal of the Economic Policy Institute's analysis of the STEM workforce.
3D printing is a new technology that raises old policy questions.
This fact sheet lists 53 documented economic benefits of IT, from jobs and output to competitiveness and...
A robust reauthorization of the America COMPETES Act can boost U.S. economic competitiveness and growth.
Policy decisions about IT in the vehicle should be driven by a broad government mandate to foster innovation...
China's solar trade policy hampers the clean energy economy and energy innovation.
Despite new statistics from the Census Bureau, wage and unemployment data show that we still suffer from a STEM shortage.
Data can be a key to enhancing global development.
Companies continue to face challenges securing IP rights in India, despite campaign pledges.
India simply cannot usher in a new, innovative economy if it continues to revert back to these same tried-and-failed mercantilist practices.
What is the real impact of productivity on employment?
The U.S. Ex-Im Bank is vital for supporting exports by Boeing to markets where finance would be otherwise unavailable.
We must improve our innovation foundation or risk falling further behind economically.
The technology will lead to better products and more loyal customers.
This legislation can help our government adapt to changing technologies, cut waste, and compete with the private sector for IT jobs.
Doug Brake participated in the MMTC's Telecom Act Rewrite and the Potential Benefits to MWBEs and...
Daniel Castro will speak about the NSA's impact on the economy, information security, and Internet freedom at...
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This panel discussion investigated methods for speeding the path from research to market.
Matthew Stepp gave a speech at the 2013 Regional Meeting of the Federal Laboratory Consortium in Leesburg, VA.
Daniel Castro will speak at a webinar for the Association of Public Data Users on "Public Policy...
Stephen Ezell participated in a panel on Electronic Commerce and Data Privacy at the Frontiers of Economic...
Robert Atkinson presented on IT policy issues at this event sponsored by the Copenhagen Business School.
A panel of experts discusses the truths and misconceptions of the DCMA.
ITIF testifies in Vermont against legislation sought by organic advocates to grow their market by misleading...
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ITIF testimony before the MD Legislature shows legislation to mandate labels for genetically improved foods...
Mandatory GMO Labeling legislation proposed in MD would undermine the objectives it seeks to advance.
Stephen Ezell testifies that India's innovation mercantilist policies damage India's, America'...
ITIF urged an update to the Communications Act that will sustain the amazing innovation in our networks and...
The FTC should embrace a flexible and modern regulatory regime that fosters data-driven innovation in the...
Rob Atkinson files comments to new FCC chair on future of telecom policy.
ITIF recommends that policymakers preserve statutory damages for large-scale infringement, allow the private...
The U.S. freight transportation system faces major challenges and new policies are required to enhance...
Conventional economics provides an increasingly poor guide for economic policy and should be replaced by an evolutionary economics framework.
Rob Atkinson contributed the chapter "Public Policy, Innovation and New Technologies" to the textbook "Guide to U.S. Economic Policy."
Robert Atkinson contributed the chapter "Innovation in Cities and Innovation by Cities" for the book "Creating Competitiveness: Entrepreneurship and Innovation Polices for Growth."
In a chapter for the new book Practicing Sustainability, Rob Atkinson wrote about sustainability from an innovation economics perspective.
"Innovation Economics: The Race for Global Advantage." This new book delivers a critical wake-up call: a fierce global race for innovation advantage is under way and the United States is running the risk of losing.
Daniel Castro contributed a chapter titled "U.S. Federal Cybersecurity Policy" in the book "Cybersecurity: Public Sector Threats and Responses."
Rob Atkinson contributed a chapter titled chapter "Who's Who in Internet Politics" for the book "The Next Digital Decade: Essays on the Future of the Internet."
Supply-Side Follies: Why Conservative Economics Fails, Liberal Economics Falters, and Innovation Economics is the Answer
Supply-Side Follies methodically debunks the common assumptions of conservative economics and demonstrates why it is a flawed doctrine that is setting up the U.S. for a major economic downturn in the near future.
Taking into account the historical record, the book discusses the shortcomings of prevailing liberal and conservative economic doctrines and lays out a new growth economics agenda aimed at maximizing the productivity and innovation-enhancing forces of the New Economy.


text:
#GivingTuesday #GreatWayToHelp #YourFavoriteCharity
Friday, Nov. 21, 2014
Mosaic has joined #GivingTuesday, an effort to harness the collective power of social media, to give a voice to people with disabilities and encourage others to discover the possibilities Mosaic offers in communities.
Coinciding with Thanksgiving and the kickoff of the holiday shopping season, #GivingTuesday inspires people to take collaborative social action to improve their local communities and give back in better, smarter ways to help create a better world.
On Dec. 2, 2014 – the Tuesday after Thanksgiving – #GivingTuesday will harness the power of social media to create a national moment to highlight giving, similar to how Black Friday and Cyber Monday have become days that are now synonymous with holiday shopping.
Ways You Can Help
- Like Mosaic and your local Mosaic agency on Facebook.
- Invite others to like Mosaic and your local Mosaic agency on Facebook.
- Share an #unselfie of why you support Mosaic using the hashtags #mosaicpossible, #GivingTuesday and #unselfie on their social networks.
- Like, share and repost these posts on Facebook leading up to and on Giving Tuesday.
Building community partnerships and personalized supports
Wednesday, Nov. 19, 2014
Job Coach Dulcie Mueller of Mosaic in Omaha started from the ground up when trying to find the right job for some people she supports. She worked with a local Dunkin' Donuts and together they created a new Dining Room Attendant position for two people who are served by Mosaic.
"There were and are some great advocates working at Dunkin Donuts that really helped to push this through," Dulcie said.
The two new Dunkin' Donuts employees are "thriving" and will soon gain more independence in their jobs, according to Kathryn Maxwell, Employment Support Coordinator at Mosaic.
"Not only are these individuals earning a paycheck they love, but they are making new friends and learning new skills," Kathryn said. "Thanks to Dulcie's hard work creating the partnership, the job coaches' hard work maintaining the partnership, and our clients' hard work at their new jobs, the possibilities with Dunkin Donuts have expanded beyond just our clients and our city and opened up possibilities in other cities."
The Dunkin' Donuts franchise owner is so pleased with the partnership that they are planning to work with Mosaic to add the position in additional Omaha and Kansas City locations.
Congratulations to Dulcie and the team at Mosaic in Omaha for making community connections that lead to personalized supports for people.
In the photo, Christina Hernandez (right) greets Lisa Meeks as she enters Dunkin' Donuts.
Fight the Flu
Friday, Nov. 7, 2014
Flu season is starting and it is good to be prepared to fight the flu. The flu (short for influenza) is highly contagious and can cause severe illness and hospitalization or even death.
Some facts about the flu:
- According to experts, people with flu can spread it to others up to about six feet away through droplets made when people with flu cough, sneeze or talk.
- It is also possible that a person might also get flu by touching a surface or object that has flu virus on it and then touching their own mouth or nose.
- Some people, such as older people, young children, and people with certain health conditions are at high risk for serious flu complications.
- You can pass the flu to someone else before you know you are sick, as well as while you are sick; healthy adults can infect other people beginning one day before symptoms develop and up to five to seven days after becoming sick.
- Children may pass the virus for longer than seven days.
How to fight the flu:
- The first and best step is to get vaccinated. The Centers for Disease Control and Prevention recommends that everyone over the age of six months be vaccinated annually.
- Wash your hands often with soap and water. If soap and water are not available, use an alcohol-based hand rub.
- Frequently touched surfaces should be cleaned and disinfected at home and work, especially if someone is ill.
- Stay home if you are sick.
Safety is one of Mosaic's four values. Together let's work to keep the people served by Mosaic, and one another, well and healthy.
Don't forget to take care of benefit enrollment … and take care of yourself
Monday, Nov. 17, 2014
Benefit enrollment: December 1 is the deadline for benefit enrollment for next year. Make sure to go online to the "Self Service" link on mymosaicinfo.org. On that site you'll find "HR in Touch" under the Benefits section. There you can update your benefits choices for 2015. If you have any questions about it, please contact your local Human Resources representative.
Health care fact: November is Diabetes Awareness Month. Diabetes is a serious disease that can lead to heart disease, blindness, kidney failure, nerve disease, amputations, and even death. More than 25 million people in the United States are living with diabetes, one-third of them undiagnosed.
People have been diagnosed with prediabetes or diabetes even when they had no symptoms. Because of this, the American Diabetes Association recommends that you have your blood sugar checked every three years beginning at age 45. Have it checked sooner if you are overweight and are inactive, have family members with diabetes, or have other risk factors. If you have prediabetes, you should be checked for diabetes every year or two after your diagnosis. Talk to your healthcare provider to see if you should be tested.
Learn more about Preventing Diabetes, Ways to Lower your Blood Sugar and Diabetes Awareness Month.
The photo shows various blood glucose monitors, a familiar tool for people with diabetes.
Faithful to the Journey
Leaders who are passionate and inspired can embrace uncertainty, find good, and learn from the journey, said CEO Linda Timmons on Tuesday to Mosaic leaders gathered in Omaha for the 2014 David A. Jacox Leadership Event. Noting that Mosaic's guide is "Forward in Faith: a vision for Mosaic's second century of service," and the navigation system is Mosaic 2.0, our strategic plan. Linda said, "We have the roadmap, even if we don't know all of the answers."
In her talk, titled "Living in the Questions," Linda asked five questions: Have you ever been a stranger? Are we there yet? Do I matter? Will there ever be enough? So what? Sheused personal experiences to share that even difficult, challenging and unplanned things can bring very good results. Several years ago on a trip to Africa for Mosaic, she discovered how vulnerable a stranger can feel. Yet, the openness of others to her was almost a revelation. "The people most likely to befriend another are those who have felt what it is to be a stranger," she said.
She also talked of her recent sabbatical and how the best-laid plans did not work out. A retreat took an unexpected direction and was cut short; a planned vacation to London was canceled because her mother broke her hip and was hospitalized; her son became gravely ill; the basement of her home flooded – not once, but twice; flights turned into multiple-day delays; and more. But it wasn't bad, she said – it was life.
The family spent far more time together as a whole, including a week-long driving trip. She canned jams and jellies with her daughter, helped her mother through the hospitalization, helped her husband with his business (which she doesn't otherwise have time to do), and landscaped the backyard.
It was not according to plan. But it was good. Forward in faith!
(Click on the photo for a full-sized image.)


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Immunogenicity of an E1-deleted recombinant human adenovirus against rabies by different routes of administration.
ABSTRACT The immunogenic properties of an E1-deleted, human adenovirus type 5 (Ad5) vaccine virus with activity against rabies were examined in mice, foxes and dogs using different routes of administration. NMRI mice received 10(5.8), 10(5.3), 10(4.3), 10(3.3) and 10(2.3) TCID(50) by peroral or intramuscular (i.m.) administration. Furthermore, six mice received 10(5.8) TCID(50) intracerebrally (i.c.). The construct elicited marked seroconversion in mice after oral administration. Immunoreactivity in mice was even more pronounced i.m. and i.c. After direct oral administration (10(8.0) TCID(50)) in foxes, six of eight animals developed rabies virus-neutralizing antibodies (VNA). All foxes immunized by direct injection (10(7.7) TCID(50)) in the membrane of the jejunum were shown to seroconvert. Pre-existing immunity against canine adenovirus did not hinder the development of rabies VNA after oral application of the construct (10(8.0) TCID(50)). Fox cubs (24-29 days old) born from rabies-immune vixens were shown to develop very high levels of rabies VNA after i.m. administration (10(8.0) TCID(50)), indicating that the immunogenicity of the construct could surpass maternally transferred immunity. In dogs, the construct (10(8.0) TCID(50)) induced a very strong immune response after i.m. administration. However, no immune response was detectable in dogs after direct oral administration (10(8.3) TCID(50)) or after endoscopic deposition in the smaller intestine (10(8.0) TCID(50)). Hence, it must be concluded that the construct is not suitable for oral vaccination of dogs against rabies.
- [Show abstract] [Hide abstract]
ABSTRACT: Oral rabies virus (RV) vaccines are used to immunize a diversity of mammalian carnivores, but no single biological is effective for all major species. Recently, advances in reverse genetics have allowed the design of recombinant RV for consideration as new vaccines. The objective of this experiment was to examine the safety, immunogenicity and efficacy of recombinant RV vaccines administered to captive dogs by the oral route, compared to a commercial vaccinia-rabies glycoprotein (V-RG) recombinant virus vaccine. Animals consisted of naive purpose-bred beagles of both sexes, and were 6 months of age or older. Dogs were randomly assigned to one of six groups, and received either diluent or vaccine (PBS; V-RG; RV SN10-333; RV SPBN-Cyto c; RV SPBNGA; RV SPBNGAGA), with at least six animals per group. On day 0, 1 ml of each vaccine (or PBS) was administered to the oral cavity of each dog, at an approximate concentration of 10(8) to 10(9) TCID50. After vaccination, dogs were observed daily and bled weekly, for 5 weeks, prior to RV challenge. No signs of illness related to vaccination were detected during the observation period. Excluding the controls, RV neutralizing antibodies were detected in the majority of animals within 1-2 weeks of primary vaccination. Thereafter, all dogs were inoculated in the masseter muscle with a street virus of canine origin. All control animals developed rabies, but no vaccinates succumbed, with the exception of a single dog in the V-RG group. Review of these preliminary data demonstrates the non-inferiority of recombinant RV products, as concerns both safety and efficacy, and supports the suggestion that these vaccines may hold promise for future development as oral immunogens for important carnivore species, such as dogs.Virus Research 08/2005; 111(1):101-5. · 2.75 Impact Factor
Article: Immune responses of recombinant adenoviruses expressing immunodominant epitopes against Japanese encephalitis virus.[Show abstract] [Hide abstract]
ABSTRACT: Japanese encephalitis virus (JEV), which belongs to the family Flaviviridae, causes infection of the central nervous system in humans and equines and stillbirths in swine. In the present report, we constructed and characterized the immune responses conferred by recombinant adenoviruses expressing JEV E epitopes (six amino acid residues 60-68, 327-333, 337-345, 373-399, 397-403 and 436-445 in E, designated TEP). Seven groups (n = 10) of female BALB/c mice received intramuscular (IM) or oral immunization with the recombinant adenoviruses twice at 2-week intervals. Intramuscular immunization of mice with rAd-TEP generated greater titers of anti-JEV antibodies and JEV neutralizing activity than in animals with oral injection. It statistically significant differences were found in anti-JEV antibody titers and JEV neutralizing activity induced by IM immunization with rAd-TEP at a dose of 1 x 10(8.0)TCID50 when compared with the doses tested (3 x 10(7.0) and 1 x 10(7.0)TCID50) IM inoculation of rAd-TEP. Splenocytes from mice immunized intramuscularly with rAd-TEP secreted the largest amounts of interferon-gamma and interleukin-2 and moderate amounts of interleukin-4 in the presence of JEV. It demonstrates that IM immunization with rAd-TEP induced the highest level of cell-mediated immune responses and the higher level of JEV-specific humoral immune responses than oral immunization. Then we further evaluated the protective efficacy of the recombinants in swine. All swine were protected from viral challenge with IM rAd-TEP at 1 x 10(10.0)TCID50, even though the neutralizing antibody titers were lower than those in the group inoculated with inactivated vaccine. Our findings indicate that rAd-TEP might be an attractive candidate vaccines for preventing JEV infection.Vaccine 10/2008; 26(46):5802-7. · 3.49 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: An Irkut virus (IRKV) was recently isolated from a bat in China. The protective ability of rabies biologics available in the Chinese market and experimental biologics were assessed in a hamster model against the rabies virus (RABV) and IRKV via pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP) experiments. The results demonstrated that a single dose of rabies vaccine did not induce adequate protection against IRKV. However, routine PrEP with three doses of a vaccine induced complete protection against IRKV. Higher doses of rabies immune globulins and interferon α were required during PEP to protect hamsters against IRKV compared to RABV. Experimental recombinant vaccines containing IRKV glycoprotein induced more reliable protection against IRKV than against RABV. Those may be explained by limited cross-neutralization of these viruses (confirmed via in-vitro tests) in conjunction with antigenic distance between RABV and IRKV. These results indicate that development and evaluation of new biologics for PrEP and PEP are required to ensure sufficient protection against IRKV in China and other territories where this virus is present.Journal of clinical microbiology 08/2013; · 4.16 Impact Factor
Page 1
Journal of General Virology (2001), 82, 2191–2197.
Printed in Great Britain
Immunogenicity of an E1-deleted recombinant human
adenovirus against rabies by different routes of administration
Ad Vos,1Andreas Neubert,1Elke Pommerening,1Thomas Mu ? ller,2Leopold Do ? hner,3
Larissa Neubert1and Kenneth Hughes4
1Impfstoffwerk Dessau-Tornau GmbH, PO Box 214, 06855 Rosslau, Germany
2Institute for Epidemiological Diagnostics, Federal Research Centre for Virus Diseases of Animals, Seestrasse 55, 16868 Wusterhausen,
3Micromun GmbH, Walther-Rathenau Strasse 49a, 17489 Greifswald, Germany
4Microbix Biosystems Inc., 341 Bering Avenue, Toronto, Ontario, Canada M8Z 3A8
The immunogenic properties of an E1-deleted, human adenovirus type 5 (Ad5) vaccine virus with
activity against rabies were examined in mice, foxes and dogs using different routes of
administration. NMRI mice received 105?8, 105?3, 104?3, 103?3and 102?3TCID50by peroral or
intramuscular (i.m.) administration. Furthermore, six mice received 105?8TCID50intracerebrally
(i.c.). The construct elicited marked seroconversion in mice after oral administration. Immuno-
reactivity in mice was even more pronounced i.m. and i.c. After direct oral administration (108?0
immunized by direct injection (107?7TCID50) in the membrane of the jejunum were shown to
seroconvert. Pre-existing immunity against canine adenovirus did not hinder the development of
rabies VNA after oral application of the construct (108?0TCID50). Fox cubs (24–29 days old) born
from rabies-immune vixens were shown to develop very high levels of rabies VNA after i.m.
administration (108?0TCID50), indicating that the immunogenicity of the construct could surpass
maternally transferred immunity. In dogs, the construct (108?0TCID50) induced a very strong
immune response after i.m. administration. However, no immune response was detectable in dogs
(108?0TCID50). Hence, it must be concluded that the construct is not suitable for oral vaccination of
dogs against rabies.
Oral vaccination of foxes (Vulpes vulpes) against rabies has
been developed as a preferred method to control, and
eventually to eradicate, vulpine rabies (Sto ? hr & Meslin,
1996; Mu ? ller & Schlu ? ter, 1998). This approach has also been
those that cannot be vaccinated by the parenteral route.
However, due to the close relationship between dogs and
humans, more stringent safety conditions are required for oral
vaccination of dogsagainst rabies (Wandeler, 2000). Presently,
no commercially available oral rabies vaccine is completely
without risk. In order to address this issue, new vaccine
Author for correspondence: Ad Vos.
Fax ?49 34901 885797. e-mail ad.vos?idt-direct.de
candidates have been under investigation. One such family of
candidates are recombinant human adenovirus type 5 (Ad5)
vectors that express the highly immunogenic rabies gly-
coproteinG (RG) (Yaroshet al., 1996; Xiang et al., 1996; Wang
et al., 1997). One of these prototype vaccines has already been
shown to induce protective immunity in animals upon
challenge with rabies virus (Prevec et al., 1990; Charlton et al.,
1992). However, the potential for in vivo replication of this E3-
inserted recombinant adenovirus is an undesirable attribute
from a safety perspective. In response to this shortcoming, a
replication-deficient, E1-deleted, human Ad5 vector has been
designated human Ad5 HCMV–intron–ERA, was evaluated
for its ability to induce an immune response by different routes
of administration in mice (Mus musculus), foxes and dogs. It
was also determined whether pre-existing canine adenovirus
Page 2
A. Vos and othersA. Vos and others
(CAV)-specific immunity inhibited the development of rabies
Finally, the ability of the construct to surpass maternally
transferred immunity against rabies in young animals was
examined. The variability of construct immunogenicity in
terms of target species and route of administration was
? Vaccine virus. The vaccine virus was produced by a homologous
Biosystems Inc., Toronto, Canada). Briefly, cDNA for the ERA (Evelyn–
Rochitniki–Abelseth) strain RG was inserted into the XbaI site of plasmid
pCA13 just downstream of the forward-orientated HCMV immediate-
early promoter and ahead of the SV40 polyadenylation signal. This
generated plasmid pCA13-RG.
by Berkner & Sharp (1985), is known to increase protein expression in
multiple systems. It was cloned into pCA13-RG, downstream of the
promoter region and before the RG gene. The intron sequence in
pMLPsp1a contains the 5? splice-donor site from the Ad2 major late
promoter (MLP) fused to the 3? acceptor site from an immunoglobulin
light-chain gene. The primary sequence of the intron is as follows: 5?
The intron was extracted from pMLPsp1a by PCR under standard
conditions. The PCR product was restricted with EcoRI and ligated into
similarly cut pCA13-RG to generate pCA13(int)-RG. Nucleotide orien-
tation was confirmed by sequencing.
In order to produce recombinant human Ad5 bearing the HCMV–
intron–ERA cassette, pCA13(int)-RG was co-transfected with plasmid
pBHGE3, which contains the right-hand end of Ad5 (rightward of E1),
into 293 cells. 293 cells are a human kidney cell line that has been
transformed with left-end regions of Ad5, including E1. Homologous
recombination between pBHGE3 and pCA13(int)-RG results in the
production of a viral genome that will replicate in 293 cells and produce
progeny virus. 293 cells provide helper E1 function. Individual virus
isolates are selected by plaque purification on immobilized 293 cell
HCMV–intron–ERA virus was propagated routinely in suspension
culture using 293N3S as the host cell line. For cultivation, the suspension
cells were placed in spinner flasks containing growth medium (MEM
Eagle, Joklik modification, Sigma) with 10% foetal calf serum. Virus was
harvested 3 days after infection of cells. HCMV–intron–ERA virus was
enumerated by TCID??on 293N3S cells.
? Animals. All animals were without detectable levels of rabies virus-
neutralizing antibodies (VNA) prior to vaccination, with the exception of
the fox cubs born from rabies-immune vixens. Foxes were marked
individually with electronic identifiers and juvenile specific-pathogen-
free dogs (beagles) were identified individually by a tattoo (ear). All
animal tests were conducted at the Experimental Animal Facility of
Impfstoffwerk Dessau-Tornau GmbH (IDT). Animal experimentation
was performed according to the German Animal Welfare Act of 25 May
1998. The experimental design of the tests, as required, was approved by
the appropriate German authorities.
? Rapid fluorescence focus inhibition test (RFFIT) assays
Rabies virus. Prior to testing, sera were heat-inactivated at 56 ?C
for 30 min and then centrifuged at 926 g for 5 min. Serum samples
were evaluated for rabies VNA by the RFFIT as described by Smith et
al. (1973), with the modifications described by Cox & Schneider (1976).
The rabies VNA titres were converted to international units (IU). The
international standard immunoglobulin (2nd human rabies immuno-
globulin preparation, National Institute for Standards and Control,
Potters Bar, UK), adjusted to 0?5 IU?ml, served as a positive control
for these experiments. A level of 0?5 IU?ml is as an arbitrarily defined
threshold indicative of protection against rabies infection (Anonymous,
1978) and is used here as the threshold for positivity.
CAV2. A simple inhibition test (Mayr et al., 1977) was used to test
sera for VNA against CAV2. Firstly, the serum samples were inacti-
vated at 56 ?C for 30 min. A virus suspension with a known titre was
added to each serum dilution and the mixture was incubated at 37 ?C
for 60 min. This mixture was then inoculated on monolayers of recep-
tive Madin–Darby canine kidney (MDCK) cells for virus replication.
Four days later, CAV2-neutralizing antibody titres were evaluated ac-
cording to Spearman–Ka? rber, based on the CPE. A titre of 1:4 was
considered negative.
? ELISA. The objective of these tests was to detect both general
for human Ad5. For detection of the former, highly purified human Ad5
hexon protein was used. This protein was isolated by DEAE chromato-
graphy and crystallization (Do ? hner & Hudemann, 1972). The hexon
proteinis the most abundant adenovirusprotein (Zabner et al., 1997), and
antibodies directed against this protein could also be the result of earlier
CAV infection. The Ad5-specific ELISA was conducted with highly
purified human Ad5 fibre antigen. Fibre antigen was isolated by anion
exchange and immunoaffinity chromatography.
The ELISA was performed under standard conditions using hexon or
fibre antigens, at 1 µg?ml, for coating microtitre plates. Peroxidase-
labelled protein A was used as the indicator reagent. Human antibodies
were used as the positive control. Dog, fox and human antibodies react
similarly with protein A (Yamamoto et al., 1985). The absorbances of
'cut-off values' at 450?620 nm for specific antibodies were in the range
of 0?5. Adenoviruses are ubiquitous; hence, it is to be expected that
animals have circulating antibodies against adenoviruses. Serum samples
from mice were diluted 1:10 to allow the detection of low reactivity
between IgG and protein A.
? Statistics. Student's paired t-test was used to detect possible
intraspecific significance in absorbance differences between different
blood samples (Zo ? fel, 1988).
Study 1. Mice [peroral (p.o.), intramuscular (i.m.) and
intracerebral (i.c.)]
Three groups of six mice were given 0?02 ml human Ad5
HCMV–intron–ERA (10??? TCID??) p.o., i.m. or i.c. Animals
that received the construct i.c. were anaesthetized by intra-
peritoneal (i.p.) injection with a 1:10 dilution of ketamine
hydrochloride in saline. The animals vaccinated p.o. drank the
construct voluntarily from a syringe. During a subsequent trial
in mice, four groups of 10 animals each received 0?02 ml of the
construct using different concentrations (10???, 10???, 10??? and
10??? TCID??). Five animals of each group received the
construct orally, the other five by the parenteral route (i.m.).
Page 3
Recombinant adenovirus against rabiesRecombinant adenovirus against rabies
Table 1. Rabies VNA titres of mice inoculated with human Ad5 HCMV–intron–ERA by different routes of administration
Five or six animals were inoculated with the doses shown. Titres for individual animals are given in IU?ml, as determined by RFFIT. (–), No rabies VNA detectable (?0?5 IU?ml); ,
not done.
Dosage (TCID50)
Route of
269?2, 226?3, 226?3, 269?3, 320?1, 269?2
67?3, 56?6, 56?6, 80?0, 190?3, 80?0
20?0, 20?0, 40?0, (–), 80?0
33?6, 5?0, 10?0, 8?4, Died*
(–), 56?5, 4?2, (–), 0?9
(–), (–), (–), (–), (–)
10?0, 7?1, 10?0, (–), 67?3, 5?9
47?6, 7?1, (–), 67?3, (–)
(–), 10?0, (–), (–), (–)
(–), (–), (–), (–), (–)
(–), (–), (–), (–), (–)
* Animal died 2 days post-vaccination; cause of death unknown, but unrelated to rabies.
Table 2. Rabies VNA titres of foxes inoculated orally with
108?33TCID50human Ad5 HCMV–intron–ERA
Titres are given in IU?ml, as determined by RFFIT. (–), No rabies
VNA detectable (?0?5 IU?ml); , not done.
Fox B0B1 B2 B3 B4
This time, the construct was applied orally with a perfusion
needle in the posterior part of the oral cavity of the animals.
For this purpose, the mice were anaesthetized in an ether jar
prior to administration of the construct. All mice were bled by
retro-orbital puncture and subsequently euthanized 55 or 60
days post-immunization. Blood samples were examined for
serum antibody titres against rabies by using RFFIT and
antibodies against the human Ad5 fibre and hexon proteins
The immune responses against rabies of mice inoculated
with human Ad5 HCMV–intron–ERA vaccine are shown in
Table 1. The highest levels of rabies VNA were observed in
themice inoculatedi.c.Theimmune responsein mice receiving
the human Ad5 HCMV–intron–ERA construct orally was
inferior to mice immunized i.m. with the same dose. It was also
observed that the seroconversion rate was dose-dependent.
With the exception of three (of six) mice vaccinated i.c., none
of the animals showed high levels of antibodies against the
fibre and hexon protein (data not shown).
Study 2. Foxes (p.o.)
Eight juvenile foxes received 10??? TCID??human Ad5
HCMV–intron–ERA by direct oral instillation. Blood samples
werecollected 0 (B0), 21 (B1), 35 (B2), 70 (B3) and 95 (B4) days
post-vaccination. All blood samples were examined for the
presence of rabies virus- and CAV-neutralizing antibodies by
RFFIT. Detection of antibodies against the human Ad5 fibre
and hexon proteins was also performed by ELISA.
Six of the eight foxes seroconverted (threshold 0?5 IU?ml).
The geometric mean titres (GMT) of the sera of immunized
foxes were 2?75 (B1), 2?13 (B2), 1?33 (B3) and 1?48 (B4) IU?ml
(Table 2). None of the animals had detectable levels of CAV-
neutralizingantibodies at any timeduring the study. The mean
absorbances against the fibre protein of serum samples B0 and
Page 4
A. Vos and othersA. Vos and others
Table 3. CAV2 VNA titres of foxes pre-immunized with
Titres were determined by RFFIT. (–), No CAV2 VNA detectable
B2 were 0?296?0?071 and 0?415?0?112, respectively. For
the hexon protein, the following mean absorbances for B0
and B2 were obtained: 0?779?0?361 and 1?439?0?724. The
levels of antibodies against the hexon and fibre proteins of
?2?43, d.f.?7, P?0?05).
Study 3. Foxes (intrajejunal)
Five cubs, 8 weeks old and born from rabies-naı?ve vixens,
received by injection 0?5 ml human Ad5 HCMV–intron–ERA
(10??? TCID??) in the membrane of the jejunum following
surgical exposure (laparotomy). For this purpose, the animals
were sedated with a ketamine–xylazine (10%?2%) solution.
Blood samples were collected 0 (B0), 21 (B1) and 49 (B2) days
post-vaccinationand examinedfor the presenceof rabies VNA
All five foxes had detectable levels of rabies VNA. Sera
GMT for the vaccinated foxes were 32?2 (B1) and 11?9 (B2)
Study 4. Foxes (p.o.) vaccinated against CAV2
The effect of pre-existing CAV-specific immunity on the
immune response against rabies, induced by the construct, was
investigated by administering 10??? TCID??CAV2 antigen
(produced at IDT) i.m. to four adult foxes after a blood sample
(B0) had been taken. Twenty-nine days later, the foxes were
bled (B1) and subsequently received 10??? TCID??human Ad5
HCMV–intron–ERA vaccine by direct oral instillation. Blood
samples were taken 28 (B2) and 55 (B3) days after adminis-
tration of the construct. The blood samples were examined for
the presence of rabies and CAV VNA by RFFIT.
All four foxes developed detectable levels of CAV2 VNA
after vaccination with CAV2 antigen and prior to adminis-
tration of the construct (B0 and B1) (Table 3). All animals pre-
immunized with CAV2 developed rabies VNA upon oral
immunization with the construct. The GMT were 3?61 and
3?27 IU?ml at 28 (B2) and 55 (B3) days post-administration.
These results indicate that antibodies to CAV2 did not inhibit
the immune response to rabies after vaccination with the
Study 5. Fox cubs (i.m.)
In order to investigate whether maternally transferred
immunitytorabies viruswouldimpairthe immuneresponse to
RG upon vaccination with the human Ad5 HCMV–intron–
ERA construct, 13 cubs born from four rabies-immune vixens
were given the construct i.m. The vixens had been vaccinated
orally with SAD (Street Alabama Dufferin) B19 just before
mating. SAD B19 is a live-modified rabies virus vaccine, used
extensively for oral vaccination of wildlife (Schneider & Cox,
(mean 24?3 days), were bled (B0) and subsequently injected
i.m. with 10??? TCID??human Ad5 HCMV–intron–ERA.
Blood samples were collected 14 (B1), 21 (B2), 28 (B3) and 47
(B4) days post-vaccination and examined for the presence of
rabies VNA (RFFIT).
The rabies VNA titres of the four vixens at the time of
vaccination of the cubs were 1?81, 11?02, 11?88 and 5?12 IU?
ml. All cubs born from these rabies-immune vixens developed
rabies VNA upon immunization with the construct. The GMT
of the sera of the cubs were 0?38 (B0), 31?08 (B1), 108?73 (B2)
and 155?26 (B3) IU?ml.
Study 6. Dogs [p.o., i.m. and gastrointestinal (g.i.)]
Six dogs received 10??? TCID??human Ad5 HCMV–
intron–ERA by direct oral instillation. Blood samples were
collected 0 (B0), 21 (B1), 35 (B2), and 118 (B3) days post-
vaccination. After the first oral instillation, none of the animals
seroconverted. Hence, vaccination was repeated using a
different lot of vaccine virus. Vaccination was repeated with
10??? TCID??, four animals by the oral route and two by
parenteral (i.m.) administration. Dogs were bled 28 (B4) and 55
(B5) days after this second vaccination attempt.
The dogs that received the secondary vaccination by the
oral route did not develop detectable levels of rabies VNA.
Only the i.m.-inoculated animals seroconverted, with high
levels of VNA [dog 3160, 99?00 (B4) and 160?89 (B5)
IU?ml; dog 3161, 18?80 (B4) and 41?67 (B5) IU?ml]. Dogs
3160 and 3161 also showed a clear immune response against
route. The other four orally vaccinated dogs showed no
detectable immune response against these proteins (Fig. 1).
The four dogs that remained seronegative after secondary
oral administration received 10??? TCID??
HCMV–intron–ERA by direct intestinal instillation (endo-
scopic deposition) in the duodenum. The animals were
anaesthetized with a ketamine–xylazine (10%?2%) solution;
pre-medication was with atropine–diazepam. Blood samples
human Ad5
Page 5
Recombinant adenovirus against rabiesRecombinant adenovirus against rabies
Fig. 1. Absorbances of serum samples (B0, B2–B5) from dogs inoculated
with 108?33and 108?0TCID50human Ad5 HCMV–intron–ERA during
primary and secondary vaccination against the human Ad5 hexon (a) and
fibre (b) proteins, as determined by ELISA. Samples were included from
dogs that received the construct p.o. during both primary and secondary
vaccinations (?) and from dogs that received the construct respectively
p.o. and i.m. during primary and secondary vaccinations (?).
Blood samples were examined by RFFIT for the presence of
rabies (B0–B8) and CAV (B0–B5) VNA. Antibody levels
against the human Ad5 fibre and hexon proteins were also
determined by ELISA (B0–B5).
VNA after endoscopic deposition of the HCMV–intron–ERA
construct in the duodenum.
An effective yet safe rabies vaccine is needed for the oral
immunization of a variety of wild animals, as well as the oral
vaccination of (stray) dogs. Human adenoviruses have been
studied intensively as virus vectors for vaccine delivery and
gene therapy applications (for reviews, see Klonjkowski et al.,
1999; Russell, 2000), and have been shown to induce
protective responses after oral administration (Fooks et al.,
1998). The adenovirus genome has been well characterized,
vaccine vectors. This application is supported further by the
widehostrangeof human adenoviruses and the stabilityof the
by routine culture techniques.
The strong rabies immune response induced by replication-
defective adenovirus recombinants that express RG and the
absence of many of the safety risks inherent to multiplying
agents led us to test the E1-deleted human Ad5 HCMV–
intron–ERA as a candidate for oral vaccination of carnivores
against rabies. This replication-defective human Ad5 can only
be propagated in cells that express the E1-region proteins
exogenously and thereby complement the deletion in the viral
genome. This virus is unable to replicate in cells that do not
complement the deleted E1 region, but it is still able to infect
these cells and to induce the synthesis of very high levels of
foreign protein (RG). Hence, the potential spread of recom-
binant, replication-competent adenovirus (RCA) by horizontal
transmission from vaccinated to unvaccinated animals, as
mentioned by Hammond et al. (2000), is not a concern with
human Ad5 HCMV–intron–ERA. However, the E1 deletion
may not block the expression of virus genes completely
following in vivo administration (Von Seggern & Nemerow,
1999). The absence of RCA after serial passaging in 293 and
293N3S cells attests further to the safety of this virus vector.
Thereasonfor theabsence ofRCAinthe HCMV–intron–ERA
virus preparation is not understood. All primary sequence
elements required for homologous recombination have been
confirmed as present by sequencing analysis. However, the
absence of RCA is clearly linked to the presence of the intron
moiety, as other recombinant rabies vaccines containing the
frequencies in the order of 10−?–10−? after five serial passages
(M. Moore and K. Hughes, unpublished).
In contrast to studies with similar replication-defective
humanAd5 constructs (Prevec et al.,1990; Xiang &Ertl,1999),
mice developed serum antibody to rabies upon oral adminis-
tration of human Ad5 HCMV–intron–ERA. However, the
immune response of mice vaccinated orally was inferior to that
of animals vaccinated i.m. or i.c., and was clearly dose-
dependent. In rabies-naı?ve foxes, six of eight animals did
seroconvert after direct oral administration. However, the
immune response was lower than that in foxes vaccinated
P5?88, showing a sub-optimal immunization process (Neubert
et al., 2001; Schuster et al., 2001).
As exposure to CAV is relatively common in wild and
domestic canids (Garcelon et al., 1992; Laurenson et al.,
1997; Gese et al., 1997; Truyen et al., 1998; Cypher et al.,
1998; Spencer et al., 1999), it is to be expected that many
animals have developed VNA against CAV. However, pre-
immunity to CAV did not impair the ability of the human Ad5
HCMV–intron–ERAconstruct toelicitan immune response to
rabies. We have shown previously that cubs whelped by
upon oral immunization with the highly efficacious vaccine
virus SAD B19. This inhibition of the immune response
outlasted the presence of detectable levels of maternal
antibodies (Mu ? ller et al., 2001). The rabies-specific VNA
response to the human Ad5 HCMV–intron–ERA construct,
after i.m. administration in cubs whelped by rabies-immune
vixens, was significantly higher than the response elicited to
the oral rabies virus vaccine SAD B19 in cubs born from naı?ve
vixens (Mu ? ller et al., 2001). This demonstrates that maternal


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Join Us For JOYFUL SOUL FITNESSA Fun & Unique WorkshopWith Aggie Kuschner & Elsie JenkinsSunday, December 14 4:00 6:00 pm Tuition $30 Do you love Yoga and Meditation but, also love DANCING? This holistic experience will connect Body, Mind and Spirit.Join Aggie Kuschner and Elsie Jenkins for a two hour workshop designed to make you feel vibrant, deeply connect with yourself your innate healing capacity and experience unconditional joy!
Count on Yoga, Warm ups, Dancing sequence, Meditation and Gong Bath. A fun class with two forever young and happy women.
Bring a friend! Sign up today!
Attracting Love and Miracles Kundalini Yoga Workshop & DVD SigningWith KarenaSunday, March 22, 2015 12:00 pm 2:00 pm $35 The art of Kundalini Yoga is the art of transforming fear into tender devotion by vibrating at the frequency of God's all encompassing Love. With the science of Kundalini yoga, and this powerful time in our world, we have been given gifts that can make the impossible possible. Join Karena as she shares some of the ancient teachings that transform our lives, and allow Love, Abundance and Miracles to arrive in the perfect time with ease. We do not need to know how; we simply vibrate Love from our Hearts, and allow the Miraculous to draw us closer to the Blessings that are our birthrights.
Love and Miracles are everywhere for everyone, and with the attitude of gratitude and humility, we will bow our heads to Guru Ram Das. When our thoughts and emotions are aligned with God's Light, and we activate the high vibration of Love, we share a powerful frequency through our etheric bodies. This frequency becomes like a magnet drawing in goodness. Living through our Hearts, and expecting Miracles to arrive naturally with the mystical and powerful energy of our Love, is the golden key to attracting the life of our dreams.
"The greatest art is to sit, and wait, and let it come." ~ Yogi Bhajan
Introducing Xen Strength®: Yoga With WeightsWith ChrisStarting Saturday, October 4 at 8:30 am Xen Strength Yoga with Weights® is a revolutionary method that combines a creative yoga flow with strength training, to provide a transformational workout designed to sculpt, lengthen, and challenge every muscle. Youll not only get a lean yoga body, but youll also build muscle mass and increase bone density without giving up your yoga practice.
Xen Strength® builds muscle, increases flexibility, and boosts body confidence by bringing you to your edge. With Xen Strength®, you get a total-body workout, combining heart-pumping cardio benefits from a nonstop flow with an increased metabolism from added strength training in every sequence.
Whether youre an athlete or not, this method gives you an edge in your training and enhances level of fitnessbody and mind. Xen Strength® was carefully designed with the five key training essentials fitness experts recommend: cardio, flexibility, strength, core work, and balance training.
Xen Strength® has been featured on The Today Show, Dr. Oz, Self Magazine, YOUbeauty.com, Bella Life, and Bobbi Browns: Bobbi Brown: Pretty Powerful.
See you on the mat!!!
Improve Performance. Reduce Injuries and Shorten Recovery Time. For Athletes of All Levels.
Combine movement, strength training and breath work to help you excel - no matter what your sport is. High Intensity Interval Training (HIIT) using fast repetitions of yoga inspired poses to increase heartrate, burn calories and get your fire burning.
Stoke the fire with muscle-building sequences that are approachable and straightforward. Finish with poses that focus on increasing the mobility of your joints and the resiliency of your muscles.
Each class is designed to improve your balance, power, mental endurance and stamina.
Click here for more details.
Pre-registered Yogis will have priority over walk-ins. That being said we want each and every one of you on your mat, with us and practicing. If a class is getting too full for the Agni room there is also the possibility of adjusting the time so class can be held in the larger Divine Room.
Ignite is excited to present its very first Advanced Teacher TrainingWith Bir Akal Kaur (Agnes Tengerdy) and Daniele JarmanThis 500 hour Yoga Alliance Training is open to yoga teachers who hold their 200 hour teaching certificate. It is an additional 300 hours beginning October 2014 and running through June 2015.
We will meet every Friday from around 9am to 3pm. (When the Training group is formed we can review/modify schedule based on individuals needs). It will be led by Agnes Tengerdy eRYT 500 with Daniele Jarman. The training will delve into the vast healing technology of yoga. You will refine and advance your teaching skills and expand and deepen your personal practice. Get ready for the journey of a lifetime.
Applications are currently being accepted please include a letter detailing the reasons you would like to join this Training and what you would like to accomplish.
Click here for more details.
Unlimited Yoga for 2014 Twelve Months of YOGA All classes (except special workshops). Open 7 days a week!
Privates never expire. Transform yourself in 2014 into a whole new you!Agnes teaches Kundalini Privates which repairs your nervous system and Restorative Chakra Yoga Privates that are very healing especially after an illness or surgery. Please schedule directly with Agnes at 973-842-1903.
Daniele teaches privates for all ages and levels and can be scheduled via text at 973-865-5668.
Rates Class Packs: 10 pack $150 20 pack $260 Unlimited Monthly $130 per month Unlimited Yearly paid in full $1,320 = $110 per month Multi-session Private Packages: 2 one hour classes $160 4 one hour classes $280 Private yoga sessions can kick start a beginner or help an experienced Yogini work on that elusive pose.
Try a Thai Yoga Massage! Thai Yoga Massage combines elements of yoga (deep stretching), shiatsu, reflexology and acupressure working with the energy pathways of the body releasing blockages that can cause illness.The methodology is very concise, compassionate and safe. The combination is excellent for relieving muscle tension. It helps open and repair joints and connective tissue.
Thai Yoga Massage is very relaxing and meditative helping to center the mind and body. You will experience deep relaxation, stress reduction, improved circulation, increased energy, increased flexibility and improved range of motion. Treat yourself or a loved one to a Thai Yoga Massage with Roz.Click HERE for more information.
Ignite Yoga Center19 S. Livingston AvenueLivingston, NJ 07039We welcome you to our beautiful space "Ignite yoga center" and website.
We are excited to share our lovely facility and proud to offer manyclasses such as Hot Yoga, Chakra Therapy, Yin Yoga with Chakra Therapy, Kids,Teens, Beginners and others as well as all our fabulous Vinyasa Open Level classes with you.
Please come and enjoy your practice of yoga with us and ignite a change in your life.
Namaste! Daniele
Contact Us Phone: 973-597-9642 (YOGA) Daniele (cell): 973-865-5668
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Garden Sheds, Heavy Duty Workshops & Solar Potting Sheds. Display Site in Surrey
Garden Sheds Experts in Park Royal in wood, steel, plastic & concrete.
And so much more for Garden Sheds in Ruislip:
Helpful Guidance When Buying Summer Houses or Sun Room
How To Guide:- Fixing and Types of Shed Summerhouses Windows
The windows on sheds and summer houses rely on the windows to let light in so are crucial to your enjoyment of your new garden building. The way these windows are fixed to the building is also crucial so you need to be aware of the method used. The reason for this is so that you don't end up with leaking windows which can be a nuisance.
There are various ways as to how the windows are secured into place. You will often find that they are 'dry fixed' using timber beading. This is a very good way of keeping your shed or summerhouse dry provided the beading is pushed up tight against the glass. Some building only use small nails or brads to secure the glass in place and this can sometimes leak. Another option is with a mastic or sealant around the outside edge of the glass and the shed wall or even with sealant behind the glass.
When I had my garden shed company, which I started in 1979, we use to use the dry fixing method but this was supplemented with a window ledge with a recess cut for the glass to sit into. The beadings were fixed from inside the shed or summerhouse and pressed tight against the glass. This was also more secure as the beading could not be removed from outside - Continue reading --
Helpful Guidance When Buying a Garden Sheds or Heavy Duty Workshop
What Everybody Ought to Know About Garden Buildings Floors
As garden sheds and summerhouses floors are very important to the life of your new building you need to be very careful in your choice of garden sheds manufacturers. Besides the type of materials used, many of which are NOT suitable for outdoor use, you need to consider various other things to ensure your new garden shed will stand the test of time.
An important question to ask is - how thick is the floor. The very minimum size you should accept is 16mm (12mm finished thickness). This size will create a strong floor if made from proper timber, however, beware of garden shed companies offering shed floors only 10mm thick (this is 3/8's of an inch) which is smaller than a standard pencil. Just take a pencil and see how easy it breaks. This will prove to you how foolhardy it is to use boards this thin - Continue reading --
Articles from our Garden Summer Houses - Buildings Blog
Garden Sheds with a Special View.
The White Cliffs of Dover - a truly English landmark and one of the crown jewels of England - known throughout the world is home to some garden sheds with a superb view out into the English Channel. The opportunity to buy some of this supreme Kentish land with its rugged beauty and isolation don't come up very often so now is the time to bag these garden sheds, along with 13 acres of prime land. Admitted you need to have a very large pocket or to have won the lottery but someone will become the proud owner.
The Hope Bay Studio complex at Kingsdown near Deal is available on the open market. This comes with 13 acres of garden for your own personal use. Within this complex is a range of white & blue bungalows and garden sheds, also included is a unique light and airy artist's barn which feature high ceilings.
Whilst these attractive and functional garden buildings - cum garden sheds- are an incentive to buy then the truly breathtaking views from the garden extending through 180 degrees and giving a fantastic vista is surely the cherry on top - Continue reading
Visit our Garden Buildings Display site at Sutton Green Garden Nursery
situated at corner of Whitmoor Lane and Guildford Road
(A320 between Guildford and Woking)
View Larger Map
With our display site you can view our range of buildings although at the moment we do not sell
any wooden sheds or heavy duty workshops called the Park Royal but maybe in the future.


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Hello everyone.
I am new on Tamatalk and this is my first post.
First,I have a problem with my V5.
It's the first generation and I have an Onputchi,a Furikotchi and a Mumutchi.I have two V5 Tamagotchis and I have the same characters on both.
When the both tamagotchis were ready to marry,I pressed the heart,v5 and marry,then when i pressed "sure" after choosing characters the red one made a long beep and the the reset screen appeared.I pressed download and nothing was wrong but I had to wait another three days to marry them.
I haven't played with them for a while,but then I began playing with them.The white one was ready to marry,but the red one needed one more day.I was bored so I tried to connect them to play some games and win gotchi points but when I pressed "connect" the red one reseted again!I pressed download but I had to wait (again) another 3 days.
Now everytime I try to connect them the red one resets.Now I am afraid to connect them again.
What should I do?I really want to marry them togheter and raise the children at the same time.
Please help!
V5 Connection Glitch
Started by Kiki_Kiwi , Aug 13 2012 04:40 AMconnection glitch problem
Started13 Aug 2012
Last Post15 Aug 2012
Posted 13 August 2012 - 04:45 AM ( #2 )
This problem could be a sign that your Tamagotchi's battery is low. Put a new battery in the V5 that resets, and test again. Good luck!
- Kiki_Kiwi likes this
Posted 13 August 2012 - 04:48 AM ( #3 )
You're right,EMF,I have the red tamagotchi from the 2009 Christmas and I've been using it for quite a long time.The white one is newer.But my tamagotchi won't reset if i replace the battery,right?I have never replaced the battery before.
Posted 13 August 2012 - 05:56 AM ( #4 )
When you put in the battery, it will just have the reset screen and you will be able to select download like what happens when you connect; it won't erase all your data.
You're right,EMF,I have the red tamagotchi from the 2009 Christmas and I've been using it for quite a long time.The white one is newer.But my tamagotchi won't reset if i replace the battery,right?I have never replaced the battery before.
- Kiki_Kiwi likes this
Posted 15 August 2012 - 12:01 PM ( #5 )
Though I have had Tamagotchi V5s reset when I change the battery (but I think mine is glitched). Good luck changing the battery!!
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Tamagotchi iD freezes?!
Started by Myiko , 26 Oct 2014 downloads, freeze, glitch
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Jinsei Tamagotchi?
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4Us don't connect
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Tamatomo glitch ?
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Poll Favorite era of Tamagotchi characters?
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Add these shoulder exercises into your regular workout routine and increase your upper body strength, while strengthening and stabilizing your shoulders. From the stability ball dumbbell press to the medicine ball push-up, these exercises are a great way to strengthen your deltoids, rotator cuff and trapezius muscles. Browse through our large collection of detailed tutorials and step-by-step demonstrations from today's leaders in the health fitness industry. For full workout routines featuring these shoulder exercises, visit our Workouts section.
Fit Life Articles
Strength training is an important part of improving your overall fitness, and for women, it can mean much more. In addition to numerous health benefits, adding weights to your routine can become a form of personal development that builds strength in all areas of life. The...
Are you familiar with the overload training principle? Understanding this key principle allows you to create fitness programs that generate better results for your athletes while also challenging their maximal potential. Learn a variety of techniques that enable you to...
Fitness professionals and coaches who understand the demands placed on the body during performance realize how important training specificity is for their athletes. Learn why it should be considered one of the most important principles to remember when creating a training...
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|Christmas through the ages
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