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Speakers Corner
It's free to register, to post a question or to start / join a discussion
web cam
Likes # 0
Posted January 31, 2013 at 12:25PM
what happened to my post?for information about web cam.
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Posted January 31, 2013 at 12:50PM
FE has moved it to Tech Help http://www.pcadvisor.co.uk/forums/1/tech-helproom/4206091/web-cam-information/
Likes # 0
Posted January 31, 2013 at 7:45PM
With a forum name like that, and a thread title like that I thought it was another invitation like the ones I keep getting from Reese Witherspoon!
(Not really,Shopgirl,I know you for a regular!)
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So I'm considering buying a new microphone very soon. I think I want a headset mic so I get less keyboard and mouse click sounds. And up until now I've been using super cheap, breaks-in-two-months headsets, which are super cheap, break in two months and have the added bonus of sounding like total garbage with lots of background static and noise, and last year I bought a Blue Snowball which I never really liked all that much. I think it's way too quiet for me it seems. I can put it through an equalizer in post but that's not an option for live stuff, like guest appearances on streams. So it's pretty much just been a headache all the way around.
So what I'm asking is, does anybody have a microphone (preferably a headset) that they absolutely love right now? I guess I should say my budget is around 50 or 60 bucks. It's a little negotiable but that's the ball park I'm aiming for. Please and thank you guys!


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beautiful card!!!!
Send this nice and cute ecard to say thank you.
These smileys will say thank you just the way you want them.
Send this thank you greeting e-card to your friends and family and show your gratitude.
A beautiful ecard with a perfect thank you message.
Send this cute ecard to say thank you.
A thank you message for your friends for their wonderful wishes on your birthday.
Thank you card from the heart for any kind of occasion.
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A cute teddy with cute gestures says thanks.
A warm thank you e-card!
Convey your thanks to your dear ones through this bunch of roses!
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A beautiful ecard with a romantic message for your sweetheart.
Say thank you with this cute card on birthday...
Please visit my other grandstands: NainJc:http://www.youtube.com/user/NainJc...
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2 Girls 1 GameHobbies & Interests group with 78 members, open to all members
Join us every week for our definitive one-stop podcast to quench your thirst for outstanding independent games. From clever old school 8-bit freebies...
Well its obvious, that I'm a gamer. But I've always wanted to develop one now i have my chance.
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Latest tweets from @dark583
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I really wish the coroners would have cleaned up all the blood and the dam bloodied pillow.... I'm going to burn... T.co
Nov 20 2014, 3:23pm
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Just going to post it here we're meeting at applebees at 7 pass the word phone about to die
Nov 9 2014, 9:49pm
It's a sad thing to be called in the early morning to hear my dad passed away.When the officer hands you his... T.co
Nov 9 2014, 3:48pm
Coffee? What the hell is that? I take my morning drinks as Hot Cocoa
Nov 4 2014, 11:03am
The incompetence at Frys is staggering. I should have ordered from newegg.
Oct 28 2014, 7:00pm
Sooo i ended up calling Godaddy tonight and you'll never guess the gentleman I got on the phone was a gamer and... T.co
Oct 27 2014, 5:03am
haha this is pretty funny T.co
Oct 14 2014, 8:55am
Join us every week for our definitive one-stop podcast to quench your thirst for outstanding independent games. From clever old school 8-bit freebies...
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This group has been formed to gather the people who enjoy Japanese anime and all of its facets, to salute the people from the land of the Rising Sun.
A group for those without religion, as well as those who oppose it.
Thanks for visiting the Desura Contest Group. Our goal is to promote video games on Desura with you in mind helping us do it.
The purpose of this group is to schedule DayZ gameplay events, which will make it much easier to get ahead in DayZ. Eventually, through teamwork and determination...
Desura is a community driven digital distribution service for gamers, putting the best games, mods and downloadable content from developers at gamers...
nothing much bro.just thought your work is good.you guys need any help on your mod/game ?
we would be very much interested if you would like to help?
Yea id like to help send me a pm
hello....how do you know of me?


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The 31 Most Iconic Haircuts of All Time
Browse them every day, then pick one of these eternally gorgeous celebrity haircuts and head to your stylist. Just like that, you'll be out of your hair rut for good.
Sharon Stone
Make short look soft with length in the back and choppy layers on top, like Sharon Stone had in 2007.
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November 25, 2014
Iowa House Speaker Kraig Paulsen is among the most powerful people in state government, but the Republican from eastern Iowa says he's making no commitments to politics beyond the 2012 election.
Paulsen, of Hiawatha, has served in the House since 2002 and as
speaker since Republicans won a majority in the House in 2010.
Although his position puts him in the middle of every big issue
moving through the Legislature, Paulsen insists in an interview
with The Associated Press that he has modest goals and hasn't
mapped out a long-term political future beyond running for
re-election in the fall.
Paulsen says he thinks such calculations rarely turn out as
expected, and he argues lawmakers do better if they simply support
measures they think are best for their constituents.
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GAGA TEARS more valuable than Emma Watson's hair on the black market. -Lady Gaga
Mom? I feel like I don't fit in... -Lady Gaga
Anyone else reminded Of that scene in Labyrinth? (Elton John, Lady Gaga)
Add Godga to list of talent assembled for "Machete Kills," including Charlie Sheen and Mel Gibson.
Dis gun b good!
Are you going to eat that? Or wear it? -Lady Gaga


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If battling demons and searching for the Sacred Jewel shards wasn't tough enough, Kagome also faces the battles of a very modern-day student--like preparing for a school festival and a love scene in the class play! But demons wait for no one, and soon Kagome is back in the past, encountering demon women that test the bond between Miroku and Sango, a woman mysteriously in love with Inuyasha's brother Sesshomaru, and an ancestor of the boy with a crush on Kagome in the present day!
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ARMONK, N.Y., Nov. 20, 2014 /PRNewswire/ -- IBM (NYSE: IBM) today announced that it is bringing a greater level of control, security and flexibility to cloud-based application development and delivery with a single-tenant version of Bluemix, IBM's platform-as-a-service. The new platform enables developers to build ap...
|By PR Newswire
Article Rating:
|November 21, 2012 11:31 AM EST
LONDON, November 21, 2012 /PRNewswire/ --
George Lamb provides one Brit the chance to become an instant millionaire with Roller Casino
To celebrate the launch of its sleek new mobile casino designed specifically for iPhone and iPad, Roller and TV presenter George Lamb are giving one lucky person an unbelievable 1-in-37 chance of becoming an instant millionaire with one swipe of a virtual roulette wheel.
(Photo: http://photos.prnewswire.com/prnh/20121121/577886 )
Those keen to become a high-roller and place the ultimate bet will first need to head over to the Facebook page where they will be challenged to find the hidden objects featured in a high definition Rollerville image within a Facebook app. The 40 cultural symbols and icons from popular culture, TV shows and sport will be accompanied with fun cryptic clues that will be posted daily over a ten day period. Fans that manage to find 35 images or more will unlock the final challenge: to impress the Roller panel, led by presenter George Lamb, with a display of written wit.
Get creative, as the person with the most entertaining reason for why they should have the chance to make the ultimate bet - as measured by how much it gets shared online via social channels - will be invited to a secure London venue where £1m will be up-for-grabs with one spin of the virtual roulette wheel. The winner will need to choose their lucky number between 0 and 36 for their chance to become an instant millionaire in this unbelievable bet. If the finalist doesn't pick the correct number all is not lost, a Roller Rollover will take place and £10,000 will be shared between all entrants that correctly found all 40 hidden objects and have a Roller Casino account.
George Lamb said: "It's not every day you get the chance to place a bet for £1m! It's an unbelievable opportunity; I even make a guest appearance in the Rollerville image of clues, check it out and see how many you can crack."
Ian Macleod, Senior Marketing Manager at Roller Casino, said: "What better way to celebrate the launch of the sleekest real-money mobile casino in the App Store than to give someone the chance to make the ultimate bet - a bet to beat all bets? We've brought the £1m Bet to life by creating a fantastic Rollerville image and challenge, hosted by George Lamb for the nation to get involved in. It will be interesting to see who has what it takes to make a bet of this scale with just one spin of the roulette wheel."
For your chance to enter the £1m Bet visit the Facebook page at http://www.facebook.com/RollerCasino. The Roller app is available to download for free from Apple's App Store http://www.itunes.co.uk/apps/rollercasino featuring 19 games including blackjack, roulette and slots.
Every new player is eligible for a £25 welcome bonus and a host of other promotions.
Weber Shandwick
Email: [email protected]
Tel: +44(0)20-7067-0281
Nov. 20, 2014 11:00 PM EST Reads: 1,019
Focused on this fast-growing market's needs, Vitesse Semiconductor Corporation (Nasdaq: VTSS), a leading provider of IC solutions to advance "Ethernet Everywhere" in Carrier, Enterprise and Internet of Things (IoT) networks, introduced its IStaX™ software (VSC6815SDK), a robust protocol stack to simplify deployment and management of Industrial-IoT network applications such as Industrial Ethernet switching, surveillance, video distribution, LCD signage, intelligent sensors, and metering equipment. Leveraging technologies proven in the Carrier and Enterprise markets, IStaX is designed to work ac...
Nov. 20, 2014 09:15 PM EST Reads: 1,079
"There is a natural synchronization between the business models, the IoT is there to support ," explained Brendan O'Brien, Co-founder and Chief Architect of Aria Systems, in this SYS-CON.tv interview at the 15th International Cloud Expo®, held Nov 4–6, 2014, at the Santa Clara Convention Center in Santa Clara, CA.
Nov. 20, 2014 07:30 PM EST Reads: 1,232
C-Labs LLC, a leading provider of remote and mobile access for the Internet of Things (IoT), announced the appointment of John Traynor to the position of chief operating officer. Previously a strategic advisor to the firm, Mr. Traynor will now oversee sales, marketing, finance, and operations. Mr. Traynor is based out of the C-Labs office in Redmond, Washington. He reports to Chris Muench, Chief Executive Officer. Mr. Traynor brings valuable business leadership and technology industry expertise to C-Labs. With over 30 years' experience in the high-tech sector, John Traynor has held numerous...
Nov. 20, 2014 06:00 PM EST Reads: 1,061
Bit6 today issued a challenge to the technology community implementing Web Real Time Communication (WebRTC). To leap beyond WebRTC's significant limitations and fully leverage its underlying value to accelerate innovation, application developers need to consider the entire communications ecosystem.
Nov. 20, 2014 04:45 PM EST Reads: 777
The 3rd International @ThingsExpo, co-located with the 16th International Cloud Expo - to be held June 9-11, 2015, at the Javits Center in New York City, NY - announces that it is now accepting Keynote Proposals. The Internet of Things (IoT) is the most profound change in personal and enterprise IT since the creation of the Worldwide Web more than 20 years ago. All major researchers estimate there will be tens of billions devices - computers, smartphones, tablets, and sensors - connected to the Internet by 2020. This number will continue to grow at a rapid pace for the next several decades.
Nov. 20, 2014 01:00 PM EST Reads: 1,342
The Internet of Things is not new. Historically, smart businesses have used its basic concept of leveraging data to drive better decision making and have capitalized on those insights to realize additional revenue opportunities. So, what has changed to make the Internet of Things one of the hottest topics in tech? In his session at @ThingsExpo, Chris Gray, Director, Embedded and Internet of Things, discussed the underlying factors that are driving the economics of intelligent systems. Discover how hardware commoditization, the ubiquitous nature of connectivity, and the emergence of Big Data a...
Nov. 20, 2014 12:30 PM EST Reads: 1,630
Almost everyone sees the potential of Internet of Things but how can businesses truly unlock that potential. The key will be in the ability to discover business insight in the midst of an ocean of Big Data generated from billions of embedded devices via Systems of Discover. Businesses will also need to ensure that they can sustain that insight by leveraging the cloud for global reach, scale and elasticity.
Nov. 18, 2014 09:00 PM EST Reads: 1,921
SYS-CON Events announced today that Windstream, a leading provider of advanced network and cloud communications, has been named "Silver Sponsor" of SYS-CON's 16th International Cloud Expo®, which will take place on June 9–11, 2015, at the Javits Center in New York, NY. Windstream (Nasdaq: WIN), a FORTUNE 500 and S&P 500 company, is a leading provider of advanced network communications, including cloud computing and managed services, to businesses nationwide. The company also offers broadband, phone and digital TV services to consumers primarily in rural areas.
Nov. 18, 2014 08:15 PM EST Reads: 1,435
SYS-CON Events announced today that IDenticard will exhibit at SYS-CON's 16th International Cloud Expo®, which will take place on June 9-11, 2015, at the Javits Center in New York City, NY. IDenticard™ is the security division of Brady Corp (NYSE: BRC), a $1.5 billion manufacturer of identification products. We have small-company values with the strength and stability of a major corporation. IDenticard offers local sales, support and service to our customers across the United States and Canada. Our partner network encompasses some 300 of the world's leading systems integrators and security s...
Nov. 18, 2014 08:15 PM EST Reads: 1,359
IoT is still a vague buzzword for many people. In his session at @ThingsExpo, Mike Kavis, Vice President & Principal Cloud Architect at Cloud Technology Partners, discussed the business value of IoT that goes far beyond the general public's perception that IoT is all about wearables and home consumer services. He also discussed how IoT is perceived by investors and how venture capitalist access this space. Other topics discussed were barriers to success, what is new, what is old, and what the future may hold. Mike Kavis is Vice President & Principal Cloud Architect at Cloud Technology Pa...
Nov. 18, 2014 01:30 PM EST Reads: 1,933
Smart devices that use wireless technology to exchange information with each other and with their human owners: this is the Internet of Things (IoT). This incredible level of connectivity is already transforming how we exercise, treat diseases, park our cars and access business documents. Research firm IDC projects that the IoT world of connected devices will grow to 200 billion objects by 2020. As more businesses, entrepreneurs and government entities embrace the IoT, more data will be generated daily than the already mind-boggling 2.5 quintillion bytes of data per day. However, even curren...
Nov. 15, 2014 02:00 PM EST Reads: 1,605
The Industrial Revolution in the 18th to 19th centuries was a period during which predominantly rural societies in Europe and America became industrial and urban. Advances in steam technology, transportation, mass production and the telegraph collectively transformed industry and society. Today, the Internet of Things (IoT) has the potential to once again transform industry and society just as the Industrial Revolution did. Analyst firm IDC forecasts that the IoT market will grow to $8.9 trillion by 2020 with anywhere between 30 to 50 billion connected autonomous things, making the potential g...
Nov. 15, 2014 12:00 PM EST Reads: 1,662
Cloud Expo 2014 TV commercials will feature @ThingsExpo, which was launched in June, 2014 at New York City's Javits Center as the largest 'Internet of Things' event in the world. The next @ThingsExpo will take place November 4-6, 2014, at the Santa Clara Convention Center, in Santa Clara, California. Since its launch in 2008, Cloud Expo TV commercials have been aired and CNBC, Fox News Network, and Bloomberg TV. Please enjoy our 2014 commercial.
Nov. 13, 2014 05:00 AM EST Reads: 3,497
From a software development perspective IoT is about programming "things," about connecting them with each other or integrating them with existing applications. In his session at @ThingsExpo, Yakov Fain, co-founder of Farata Systems and SuranceBay, will show you how small IoT-enabled devices from multiple manufacturers can be integrated into the workflow of an enterprise application. This is a practical demo of building a framework and components in HTML/Java/Mobile technologies to serve as a platform that can integrate new devices as they become available on the market.
Nov. 10, 2014 12:30 PM EST Reads: 2,603
The 3rd International Internet of @ThingsExpo, co-located with the 16th International Cloud Expo - to be held June 9-11, 2015, at the Javits Center in New York City, NY - announces that its Call for Papers is now open. The Internet of Things (IoT) is the biggest idea since the creation of the Worldwide Web more than 20 years ago.
Nov. 10, 2014 12:00 PM EST Reads: 5,903
Located in booth #314, the Bsquare team will present DataV demos and discuss how DataV will help customers put their data to work to improve business outcomes. DataV is unlocking new initiatives across a wide landscape of customers in industries such as industrial manufacturing, transportation, retail and mobile. The solution is designed to complement a new project start or help to enrich an existing machine investment.
Nov. 6, 2014 03:15 PM EST Reads: 2,331
The Physical Web incorporates beacons that can be put in any small retail store, for example, so that every store now has "an app" for its customers. In this Birds-of-a-Feather session at Internet of @ThingsExpo, Scott Jenson, Product Designer at Google, will discuss the Physical Web and how it is an open standard so any device can broadcast a URL wirelessly, so any phone/tablet/watch nearby can see, and rank those devices. When the user taps on one, they just go to that web page. It's really that simple. It's about thinking small, enabling micro-information (what is in my prescription bottle...
Nov. 3, 2014 05:00 PM EST Reads: 2,510
BSQUARE is a global leader of embedded software solutions. We enable smart connected systems at the device level and beyond that millions use every day and provide actionable data solutions for the growing Internet of Things (IoT) market. We empower our world-class customers with our products, services and solutions to achieve innovation and success. For more information, visit www.bsquare.com.
Nov. 3, 2014 03:00 PM EST Reads: 3,501
Whether you're a startup or a 100 year old enterprise, the Internet of Things offers a variety of new capabilities for your business. IoT style solutions can help you get closer your customers, launch new product lines and take over an industry. Some companies are dipping their toes in, but many have already taken the plunge, all while dramatic new capabilities continue to emerge. In his session at Internet of @ThingsExpo, Reid Carlberg, Senior Director, Developer Evangelism at salesforce.com, to discuss real-world use cases, patterns and opportunities you can harness today.
Nov. 3, 2014 02:30 PM EST Reads: 2,790


text:
I am distantly related to the Fenwick's of Prudhoe and Bedlington who married into the family of the Rev. Nathaniel Ellison.
William Fenwick married Mary Ellison at Bedlington 2 DEC 1725.
As the Ellisons were mentioned in a previous post I am very interested to learn what is in your document about the families.
Gen in Northumberland England
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... in the Yoshida effect. Escherichia coli cells,
Applied Microbiology and Biotechnology
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Yoshida, N.;
Ide, K.
, and chrysotile were used as recipient cells, ...
... by Shi et al. (2009a). The integrative vectors
World Journal of Microbiology and Biotechnology
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Wang, Guanglu; Bai, Ling; Wang, Zhiwen; Shi, Ting; Chen, Tao;
Zhao, Xueming
... provided by Prof. Gong Heng K. pneumoniae/
Bioprocess and Biosystems Engineering
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Zhao, Li; Zheng, Yu; Ma, Xingyuan;
Wei, Dongzhi
K-dhaT Recombinant K. pneumoniae with plasmid ...
... μl) was performed with negatively supercoiled
BMC Molecular Biology
A functional type I topoisomerase from Pseudomonas aeruginosa
Jain, Teesta; Roper, Benjamin J;
Grove, Anne
DNA (≈ 110 ng) unless otherwise stated, and the ...
... inactivation of glnA3; aprar This work
Archives of Microbiology
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.;
Engels, A.
bla, lacZ'′α complementation system Vieira and ...
... or characteristics Source or reference Plasmids
Gut Pathogens
Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance
Feeney, Audrey; Johnston, Christopher D; Govender, Rodney; O'Mahony, Jim; Coffey, Aidan;
Sleator, Roy D
Ampr, lacZ', pMB9 replicon [13] ...
... of trimers) and a DNA substrate: i) undigested
BMC Molecular Biology
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Chen, Wen-yang; Ho, John WS; Huang, Jian-dong;
Watt, Rory M
(184 ng); ii) PstI-linearized ...
... the parasite's DNA, an external control (
Journal of Venomous Animals and Toxins including Tropical Diseases
Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
Gonçalves-de-Albuquerque, Suênia da Cunha; Pessoa e Silva, Rômulo; de Morais, Rayana Carla Silva; Trajano-Silva, Lays Adrianne Mendonça; Régis-da-Silva, Carlos Gustavo; Brandão-Filho, Sinval Pinto;
de Paiva-Cavalcanti, Milena
) and an internal control (G3PD) for accurate ...
... Knabc We constructed an expression vector from
Saline Systems
NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
Kurz, Matthias; Brünig, Anika;
Galinski, Erwin
where the β-lactamase is replaced in-frame by ...
... the RNA. Materials and methods DNA and RNA The
Biotechnology Letters
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai;
Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... per recipient cell (t/r) using plasmid
European Food Research and Technology
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Rizzi, Aurora; Brusetti, Lorenzo; Arioli, Stefania; Nielsen, Kaare M.; Tamagnini, Isabella; Tamburini, Alberto; Sorlini, Claudia;
Daffonchio, Daniele
or a 1,873 bp fragment as donor DNA, and 3.5 × ...
... of a 60-base ssDNA substrate, a 60 bp dsDNA,
Nucleic Acids Research
The reverse gyrase helicase-like domain is a nucleotide-dependent switch that is attenuated by the topoisomerase domain
del Toro Duany, Yoandris; Jungblut, Stefan P.; Schmidt, Andreas S.;
Klostermeier, Dagmar
plasmid DNA, and polyU-RNA at saturating ATP ...
... by PCR. They were inserted into vector
World Journal of Microbiology and Biotechnology
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Zhu, Jian-Guo; Li, Shuang; Ji, Xiao-Jun; Huang, He;
Hu, Nan
, yielding the recombinant expression vector ...
... LSA34 Given by Dr. Zhaolan Mo pUC-gapA
Marine Biotechnology
Surface Display of Aeromonas hydrophila GAPDH in Attenuated Vibrio anguillarum to Develop a Noval Multivalent Vector Vaccine
Zhao, Yan; Liu, Qin; Wang, Xiuhua; Zhou, Lingyun; Wang, Qiyao;
Zhang, Yuanxing
derivative; Plac-gapA fusion, Apr This study ...
... and cells carrying either the empty vector
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Zhang, Hua; Wang, Zhenhui; Wang, Lei; Mu, Ren; Zou, Zhi; Yuan, Kun; Wang, Yuekun; Wu, Haiping; Jiang, Juquan;
Yang, Lifu
, or the recombinant plasmids pUCNHD ( ...
... C09G4.1 pJW9 hyl-1 seq spanning Lag1p motif*
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Tedesco, Patricia; Jiang, James; Wang, Jinqing; Jazwinski, S. Michal;
Johnson, Thomas E.
None ...
... of supercoiled, EcoRI-linearized or nicked
Nucleic Acids Research
DNA protection by histone-like protein HU from the hyperthermophilic eubacterium Thermotoga maritima
Mukherjee, Anirban; Sokunbi, Abimbola O.;
Grove, Anne
, prepared by incubation of ...
... and cells carrying either the empty vector
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Meng, Lin; Hong, Shan; Liu, Henan; Huang, Haipeng; Sun, Hao; Xu, Tong;
Jiang, Juquan
or the recombinant plasmid pUC-mrp ( ...
... materials For binding experiments plasmid DNA (
Biotechnology Letters
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Bonde, Johan;
Bülow, Leif
), linear dsDNA (linearized ...
... transformed pEC into E. coli JM83 harboring
Biotechnology Letters
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen;
Zhang, Huizhan
to be used as internal standard for evaluating ...
... A fragment of the DNA sequence of the
Measurement Techniques
Selection of a reference sequence for the determination of nucleotide sequences in DNA molecules
Golubev, S. S.; Kononogov, S. A.; Ravin, N. V.;
Skryabin, K. G.
plasmid is proposed as a reference sequence for ...
... methods Reagents and biochemicals The plasmid
Radiation and Environmental Biophysics
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Perry, Christopher C.; Urata, Sarah M.; Lee, Melissa; Aguilera, Joe A.;
Milligan, Jamie R.
was grown on a liter scale in an E. coli host ...
... putative oriT RA3 (Figure 1B) was cloned into
BMC Microbiology
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
Godziszewska, Jolanta; Kulińska, Anna;
Jagura-Burdzy, Grażyna
[23], it facilitated plasmid mobilization by ...
... was conducted as previously described 17 27 .
BMC Microbiology
Acid stress damage of DNA is prevented by Dps binding in Escherichia coli O157:H7
Jeong, Kwang; Hung, Kai; Baumler, David; Byrd, Jeffrey;
Kaspar, Charles
plasmid DNA (300 ng) was mixed with varying ...
... DNA from plasmid
Radiation and Environmental Biophysics
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Shiina, Takuya; Watanabe, Ritsuko; Shiraishi, Iyo; Suzuki, Masao; Sugaya, Yuki; Fujii, Kentaro;
Yokoya, Akinari
was irradiated with low-LET (13 keV/μm) or ...
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Additional Information
Vodafone, Red Bull and Santander: the brands driving motorsports
Vodafone, Red Bull and Santander are among the non-automotive brands seeing the advantage of reaching a young male-dominated and highly engaged audience at racing tracks across the world.
Red Bull has targeted this audience and successfully aligned itself with sporting heroes. The brand's Formula 1 team, Red Bull Racing, has won both the drivers' championship and the constructors' championship for the past three years.
Brands such as BlackBerry, Vodafone and Santander are now heavyweights on the racetrack alongside brands more traditionally associated with cars and auto sports such as Mercedes, Ferrari and Pirelli.
At the UK's annual Silverstone Grand Prix, these brands are reaching a live audience of more than 294,000 attendees over the three-day event. TV viewing figures take this figure into the millions.
The benefits to the sport are clear; with huge upfront costs meaning sponsorship is often the only way for teams to make it to competing level.
Tom Webb, head of communications for Caterham F1 Team said: "Without sponsorship, as F1 has had for something like 50 years, the sport probably wouldn't exist."
Andreas Sigl, global director of F1 at Nissan's luxury car marquee Infiniti, said Red Bull has used its brand sponsorship for mass brand exposure.
Speaking at this year's Goodwood Festival of Speed, he said: "They saw this as a way of gaining great global exposure for their brand."
Watch the full report by Joel Fothergill above, including interviews with drivers René Arnoux and Guy Smith as well as Tom Webb, the head of communications for Malaysian-owned Formula 1 team Caterham F1, and Pascal Couason, the director of Michelin Motorsport.
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Additional Information
Latest jobs Jobs web feed
- Head of Digital & Social - Dublin / London The Great & The Good In the region of £60,000 - £65,000 per annum, Dublin
- Commercial Manager Business Centric Services Group Up to £45,000 per annum + benefits, Old Street
- Head of Marketing Operations - Fixed Term Contract 12 months NEST Corporation Competitve, London
- Senior Account Manager / First Time Account Director Content is King £35k - £45k per annum dependent on experience, London (Central), London (Greater)
- Head of Acquisition & Retention NEST Corporation Competitive, London (Central), London (Greater)
- Group Account Director - Experiential Agency The Great & The Good £70,000 - £75,000 per annum, London


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Find exceptional clearance deals on digital pianos, synthesizers, electronic keyboards and more at Kraft Music. These sale items include close-outs, discontinued products, overstocks, demo units, factory recertified, and customer returns. These instruments are thoroughly inspected and tested by Kraft Music specialists to ensure they're in proper playing condition before they're made available for sale. The unit may have some minor cosmetic blemishes, but is guaranteed to function as new. You will still receive a full factory warranty, and Kraft Music backs each item with our exclusive 30-day guarantee.
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NYLO Hotels, Monscierge Offer Cloud-Based Concierge Services
Monday June 24th, 2013 - 9:49AM W
| | | | | | | | | | |
These are shortcuts to your favorite social networking and bookmark sites. Add this story to your Facebook page, del.icio.us, DiggIt, and many others!
DALLAS—NYLO Hotels LLC has introduced touch-based concierge products at all of its properties through a new partnership with Monscierge. NYLO becomes the first American boutique hotel brand to partner with the company, according to Monscierge.
Monscierge applications work by using cloud-based technology that guests can access anywhere through their iOS or Android devices. The applications are full of tips, recommendations and information provided by NYLO staff on where to go, what to do, and how to make the most of their stay.
All Monscierge applications update in real time so NYLO staff members can change or add information and alert guests to events taking place at the hotel. Once a guest has downloaded the app, it will automatically update according to which NYLO hotel the guest is visiting. Though it was designed with NYLO guests in mind, the application is free and available for download by anyone.
"We partnered with Monscierge because we wanted our guests to be able to get the very best out of their stay, 24/7," Michael Mueller, CEO of NYLO Hotels, said in a statement.
Tags: • Hospitality •
For the past few years, the talk of The Lodging Conference in Phoenix had been focused on the economic recovery, solid industry projections and "cautious optimism." With the word cautious no longer necessary, the economic outlook took a backseat this year to the seemingly unending parade of new lifestyle brands.
Hampton by Hilton to Open in Gaziantep, Turkey »
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29 results for "cameron dunkin"
Bradley-Marquez falling into placeDan Rafael
The fall showdown between welterweight titlist Timothy Bradley Jr. and Juan Manuel Marquez has moved a step closer to being finalized. Bradley signed his contract on Friday, manager Cameron Dunkin told ESPN.com. The fight is supposed to take pl...Blog | May 21, 2013
More 2012 awards in boxingDan Rafael
Yes, 2012 is in the books, but beyond last week's daily annual awards for fighter, knockout, round, prospect and fight of the year, here is part 1 of some additional awards: Trainer of the year: This was a slam dunk: Robert Garcia had a huge year ...Blog | January 02, 2013
BWAA awards ballot setDan Rafael
Over the course of two meetings, one on Dec. 1 in New York on the day the Austin Trout-Miguel Cotto fight and one on Dec. 8 in Las Vegas on the day of the Juan Manuel Marquez-Manny Pacquiao IV, members of the Boxing Writers Association of America met...Blog | December 28, 2012
Rios-Alvarado II under discussionDan Rafael
Brandon Rios sat ringside in Las Vegas two weeks ago to watch Manny Pacquiao's fourth meeting with Juan Manuel Marquez knowing full well that he was the first option for the winner to fight next, especially if Pacquiao had won. Well, we all know w...Blog | December 19, 2012
Andre Ward and Kelly Pavlik could soon meet as negotiations have ...Dan Rafael
The promoters for super middleweight champion Andre Ward and former middleweight champion Kelly Pavlik are negotiating a fight between the American stars, their promoters told ESPN.com on Friday.Story | Conversation | October 13, 2012
Elbow healed, Rios is training againDan Rafael
Former lightweight titlist Brandon Rios is back training at full strength and looking for a fall fight, according to Cameron Dunkin, the fighter's manager. Rios Rios was supposed to fight Mauricio Herrera in a junior welterweight bout July 7 on H...Blog | July 24, 2012
Brandon Rios withdraws from July 7 fight on HBODan Rafael
Former lightweight titlist Brandon Rios suffered a right arm injury and has withdrawn from his July 7 HBO fight, manager Cameron Dunkin said Thursday night.Story | Conversation | June 14, 2012
Boxer James Kirkland pulls out of junior middleweight title fight...Dan Rafael
One day after accepting a junior middleweight title fight against Saul "Canelo" Alvarez, James Kirkland pulled out of the fight on Friday, according to Golden Boy Promotions chief executive Richard Schaefer and Kirkland's co-manager, Cameron Dunkin.Story | Conversation | June 01, 2012
Nonito Donaire, Jeffrey Mathebula agree to unification fightDan Rafael
Nonito Donaire agreed Tuesday to a 122-pound title unification match with Jeffrey Mathebula of South Africa.Story | Conversation | May 22, 2012
Kelly Pavlik set for first fight in 10 monthsDan Rafael
Three months after Kelly Pavlik changed trainers and relocated to Oxnard, Calif., as part of the effort to straighten out his life and save his career, the former middleweight champion is set for his first fight in 10 months.Story | Conversation | March 08, 2012
Junior middleweight Pawel Wolak signs with manager Cameron Dunkin...Dan Rafael
New Jersey-based junior middleweight contender Pawel Wolak (29-1-1, 19 KOs) of Poland signed Thursday with manager Cameron Dunkin.Story | Conversation | July 28, 2011
James Kirkland is determined to stay on the right pathDan Rafael
James Kirkland made an error in judgment that cost him 17 months in a federal prison. Now back in the ring, he says he's determined to make the right choices and stay focused on opportunities like Saturday's fight with Nobuhiro Ishida.Story | Conversation | April 06, 2011
Nonito Donaire likely to take on Anselmo Moreno in May on HBODan Rafael
Nonito Donaire is expected to return on May 28 in an HBO main event, likely in a unification fight against Anselmo Moreno, Cameron Dunkin, Donaire's manager, told ESPN.com.Story | Conversation | March 02, 2011
James Kirkland eyes March return after serving prison sentenceDan Rafael
Junior middleweight James Kirkland is relocating to Las Vegas to train for a ring return that could come March 5 after being released earlier this month from an Austin, Texas, halfway house.Story | Conversation | January 20, 2011
Kelly Pavlik scheduled to leave rehab TuesdayDan Rafael
Former middleweight champion Kelly Pavlik, who has spent the last two months at the Betty Ford Center for treatment for alcohol abuse, is due to leave the facility Tuesday, manager Cameron Dunkin told ESPN.com.Story | Conversation | January 03, 2011
James Kirkland transferred from jail, but comeback fight called o...Dan Rafael
Junior middleweight James Kirkland was transferred from an Austin, Texas-area jail Tuesday after spending the past week there on a probation violation, co-manager Cameron Dunkin told ESPN.com.Story | Conversation | November 10, 2010


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The Environmental Protection Agency has proposed lowering the amount of ethanol and other biofuels that must be combined with U.S. fuel, according to these reports. The agency plans to require between 12.7 billion and 13.2 billion gallons of ethanol to be blended in fuel, down from 16.55 billion gallons this year. The EPA will hold a 60-day comment period to get public feedback on the move, which is seen as a response to market and infrastructure hurdles.
View Full Article in:Hill, The · Wall Street Journal (tiered subscription model), The
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This has to be the best magic site I have ever used. I must say I have been using it for a long time now, and I am even an affiliate. I hope to do...Read More ->
I am a first time user of MJM Magic. I have been performing magic for over 20 years. I was a little skeptical about using a new magic supplier, but...Read More ->


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California Gold
Signet, 2001 - Fiction - 768 pages
The Number One New York Times bestseller is back in print. Thirty years after the Gold Rush, California continues to beckon Americans westward. James Macklin Chance, a penniless wanderer, descends from the Sierras with the dream of conquering California, like the Spanish before him, and seizing his share of its wealth, no matter the price.
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Review: California GoldUser Review - Melissa - Goodreads
I like the theme of the American dream and more specifically the California dream. But it was too real. Sometimes you just want to have a story with happy endings, not a theme of broken morals and loss of faith in humanity. Read full review
Review: California GoldUser Review - Bearcatgary - Goodreads
This was a decent book of historical fiction about California in the late 1800's and early 1900's. I was never really captivated by the book, which is why it took me several months to read. I think I ... Read full review
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Baby Shower Food Ideas
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$30 - Recipes My Mother Cooked - In Recipes My Mother Cooked the country's favourite chefs have come together to share the recipes their mothers cooked for them. Whether you fancy a quick and easy dish, traditional fare or something more exotic, this is the perfect place to find all the inspiration you'll ever need to feed your family and enjoy those special mealtime moments.
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Unemployment rate declines to 8.2 percent, but…
**Written by Doug Powers
The part of the latest jobs report that the Obama administration might single out is that the unemployment rate dropped to the lowest level in three years. They won't, however, be trumpeting the reason for the drop.
There is the obligatory "unexpectedly" in the report as well:
Employers added 120,000 jobs last month, the Labor Department said on Friday, the smallest increase since October.
Economists polled by Reuters had expected nonfarm employment to increase 203,000 and the unemployment rate to hold at 8.3 percent.
The slowdown in employment growth last month likely reflected the fading boost from unseasonably warm winter weather.
The drop in the unemployment rate, to the lowest level since January 2009, reflected a drop in the labor force. The separate household survey, from which the jobless rate is derieved also showed a drop in employment.
The number of persons not in the labor force returned to an all time high, which chipped a tenth of a point off last month's unemployment number (h/t Zero Hedge):
Hiring slowed in March as well:
Also, remain on high alert for QE3, though it appears less likely now:
The painfully slow recovery in the labor market is a concern for Fed Chairman Ben Bernanke, who is keeping open the option of further monetary policy support for the economy if the unemployment rate remains stubbornly high.
The White House comments on the March jobs report: "There is more work to be done."
I was afraid they were going to say that.
**Written by Doug Powers
Twitter @ThePowersThatBe
Obama's executive amnesty helps draw thousands of Lois Lerner's emails out of the shadows
November 21, 2014 07:04 PM by Doug Powers
With a rebuttal to President Obama's amnesty announcement, here's President Obama
November 20, 2014 08:25 PM by Doug Powers
Good luck with that: St. Louis mayor agrees to protest group's demands
November 19, 2014 07:59 AM by Doug Powers
White House: Do something about 'climate change' or prepare for a fiery death — or worse
November 18, 2014 07:24 PM by Doug Powers
Jonathan Gruber cashed in even more than previously thought; Update: And Obama JUST found out about all this
November 16, 2014 09:23 AM by Doug Powers
Categories: Barack Obama, Corruption, Democrats, Health care, Nancy Pelosi


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Spring Pledge Drive SuccessPosted: April 15th 2014 @3:04 PM
The staff and volunteers at WOMR are elated by the continuing overwhelming support of our listeners. Pledge Drives for radio stations are often likened to visits to the dentist, you need 'em, you hate 'em, and they're never pleasant-to listen to or …
Spring 2014 Pledge DrivePosted: April 11th 2014 @11:34 AM
The Spring Pledge Drive is rolling along nicely thanks to all the efforts of our volunteers and listeners. You can do your part and get a souvenir at the same time. Your contribution of $75 or more entitles you to …
Pledge Drive UpdatePosted: April 9th 2014 @10:36 AM
As you can see from the pledge-o-meter to the right, we are making incredible progress toward our fund raising goal. If you haven't jumped on board yet (or even if you have), you have a chance to take home an …
Spring 2014 Pledge DrivePosted: April 6th 2014 @2:14 PM
The Spring Pledge Drive is off to a rollicking good start thanks to all the efforts of our volunteers and listeners. You can do your part to ensure the continued financial health of your community radio station and get a …
Lea DeLaria Tickets On Sale Now!Posted: April 6th 2014 @9:32 AM
We're throwing a party on Memorial Day weekend and you're invited. Get your tickets now for the biggest thing happening on a really big weekend in a really happening town. On Saturday night May 24, bask in the glow of …
Lon Chaney Birthday BashPosted: March 20th 2014 @8:41 PM
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"SWARMED???" Two hundred people is a "SWARM?" More...
Submitted by pawnstorm12 on Sun, 03/10/2013 - 15:38. Permalink
...big exaggerations from the media.
I wonder how long before the close the capitol in Olympia to anyone carrying a gun.
Presently there are no detectors - Anyone can walk right in.
I've been to several offices of state legislators - and even gave one or two a piece of my mind.
I hope they keep it open to we the people (who own it).
"We have allowed our nation to be over-taxed, over-regulated, and overrun by bureaucrats. The founders would be ashamed of us for what we are putting up with."
-Ron Paul
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My Secret Record Collection: Kate Ceberano
Arts Online
In the Secret Record Collection some of Australias leading musicians unpack their cherished vinyl collections and explain how records fuelled their love of music.
In the third part of the series, ABC Arts spends time with pop and soul singer Kate Ceberano.
Kate Ceberanos vinyl obsession started as child, fuelled by her parents record collection. She stretched herself as a young musician singing along to Linda Ronstadts Simple Dreams. In her teens Ceberano was drawn to records that communicated through the cover art as much as the music. Grace Jones Nightclubbing and Julie Londons self titled LP showed that female singers could present a strong identity through their music and their visual messaging.
Record collection favourites
The Sweet: Sweet Fanny Adams (Favourite cheesey album cover)
Abba: Voulez Vouz (most embarrassing, daggy album cover)
Fleetwood Mac: Rumours (Heartbreak Album no 1)
Kate Bush: The Kick Inside (the album that changed my life forever)
Grace Jones: Nightclubbing (the perfect dance record sums up early 80s clubbing)
Julie London: The Best Of (most evocative album cover)
Joni Mitchell: Ladies Of The Canyon (the classic journey album must be listened to all the way through)
T-Rex: Electric Warrior (perfect teenager album)
Serge Gainsbourg: Best Of (the sound of Sunday afternoons chilling with family)
Elton John: Goodbye Yellow Brick Road (album that is a guilty pleasure)
Roberta Flack: First Take (another classic heartbreak album to recover to)
Van Morrison: Moondance (the ideal album to play after a big night out)
Linda Ronstadt: Simple Dreams (the album used for voice practice and singing technique)
Produced by Tim Bosanquet
Filmed by Rachel Storey, Rachel Robinson, Rebecca OBrien
Edited by Phillipa Byers
Commissioning Editor: Nicola Harvey
Related content
Watch >> Other episodes from Secret Record Collection.
Related information
Visit >> KateCeberano.com
Comments (3)
UberRoom :
18 Jan 2014 6:20:43pm
Seriously too cool.
Kate, you have inspired me to go and get the vinyls out and enjoy them again!
DJ Dizar :
29 Dec 2013 8:17:20pm
I love this this is so cool!
I saw her as a kid at Qantas Concert, she the real deal.
Kat :
23 Dec 2013 2:42:17pm
Kate Ceberano is one of my favourite singers! Loved hearing about her favourite artists which had me cracking up with laughter. Needless to say I've hunted down some of her collection favourites. Awesome segment!


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Added Jun 3, 2013
by lolacouture
Wonderland, France
✿I didn't use pattern again✿
Jun 3, 2013, 05.14 PMby Mary Athey
cute top. love your fabric x
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Review: The High-Tech London Clap Clinic That Spots Your STDs in Seconds
Dean Street Express brings touch screens and molecular diagnostic systems to aid Soho's sexual health.
Dean Street Express brings touch screens and molecular diagnostic systems to aid Soho's sexual health.
The way hospitals are designed could improve the experiences of staff and visitors, and even the recovery of patients.
Don't feed ducks chips. They eat sausage rolls in the wild.
Stuff We Instinctively Know But Ignore Because We Can't See Or Feel The Effects Instantly.
Public health saviour or smoking's second wave? It's time to get informed about e-cigs.
"And a side order of those delicious mushroom flavoured Brussels sprouts for Timmy, please."
A personal trainer wants her Fitbit data to be used in a court of law to prove the extent of her injuries. No. Just no.
"Vape: The Sitcom" sure to follow.
But it keeps you healthy, you saucy so-and-so.
Sorry to hear about your bottom problems, but it sounds like you brought them on yourself.
A startup called Pathway Genomics is teaming up with IBM to create a fitness and diet tracking app that uses DNA sequencing and Watson's intelligence to give custom health recommendations.
From 2016, you'll be able to view your NHS health records online, while doctors will also be able to give an official seal of approval to the best health apps.
DMDM hydantoin? Ammonium lauryl sulphate? What is this stuff anyhow?
We're not quite there yet, but this sensor, which can diagnose diseases from a single drop of blood, is a pretty massive baby step.
Probably not the killer app the VR world is looking for.


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Thank you for supporting the 2014 Meals on Wheels Walkathon!
Together we raised over $99,000
to help homebound elders in our community
The Meals on Wheels program incurs a deficit every year.
We need your continuing support.
Hope to see you next year!
Saturday, May 3, 2014 at the Franklin County Technical School
"The difference between what we do and what we are capable of doing would suffice to solve most of the world's problems."— Mahatma Gandhi


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Seems like just about every major national basketball publication and website has the Bulls and Cavaliers penciled in for a late May series to decide the Eastern Conference championship.
The Bulls continue their preseason slate Monday night against the Denver Nuggets, and you can catch all the action on Comcast SportsNet at 7 p.m.
If it wasn't clear before Wednesday, it is now. Tom Thibodeau isn't blowing smoke when speaking on the importance of practice.
Jimmy Butler's stat line won't sing, but it did get fat.
It was only one game, and a preseason game at that, but Derrick Rose showed shades of his former self Monday night at the United Center.


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Friday, October 31, 2014
The Jabberwocky
If you've been around a while, you've probably noticed that I'm a little curmudgeonly about holidays and parties and anything that involves interaction with other people. But despite all the things that make me twitch, there's something I always look forward to on Halloween.
Every year we try to do a project, something that we'll remember and care about years down the road, something more than just a cute costume. Last year my three-year-old made it through the entirety of Edgar Allan Poe's The Raven. Dang, I was proud of her. The year before that, my daughter was the star of her own Halloween horror story.
This year, my four-year-old pirate princess takes a Jabberwocky's head for a prize. *
You're welcome.
*No mythical creatures were harmed in the making of this video.
Posted by neal call at 10:41 AM 2 commentsLinks to this post
Sunday, October 26, 2014
So long as we are dirty, we are pure
"All of earth is crammed with heaven
And every bush aflame with God
But only those who see take off their shoes."
-Elizabeth Barrett Browning
My daughter's birth marked a landfall after nine long months of a watery existence. Little bit by bit, each additional day she was in the womb, she became a little less fish and a little more mammal.
Sometimes, even four years later, it seems as though she's still trying to make up for her sloshing fetal isolation from terra firma. When she gleefully kicks up the dust beneath her feet, I imagine an ocean-weary mariner whose feet unexpectedly hit dry ground after so many days at sea.
She wants nothing more than to wriggle her toes in it; to savor its taste in her mouth; to pour it over her own head in a perverse mockery of acceptable cleansing rituals. But when I watch her enthusiastic, gritty exploration of the world around her, it strikes me that there is something cleansing in shucking protective barriers and getting dirty. Despite my declared love of nature, despite our move to a rural mountain town, I still spend far too much time with a window between me and the world. My four-year-old is doing her best to cure me of that problem.
* * *
At our local public library, there's a song that the kids sing during story time. It goes,
Each of us is a flower,
growing in life's garden.
Each of us is a flower,
we need the sun and rain.
Sun, shine your warmth on me.
Moon, cool me with your night.
Wind, bring the gentle rain.
Earth, take my roots down deep.
It's one of those cutesy songs that I don't want to admit enjoying. I'd be okay if most nursery rhymes and children's songs faded into obscurity. Yet I can't deny there is not much that is better than watching a bunch of three or four-year-olds perform a choreographed dance while crooning "each of us is a flower . . ."
* * *
There are treasures down there beneath the dirt. Bottlecaps, old nails, crumbling pieces of brick and ancient tile work and pottery. Worn pennies and forgotten plastic bracelets. Little rocks that could be hidden gems and big rocks that could be prehistoric fossils.
|"Getting buried," her new favorite activity
And it feels good to have my daughter by my side. She digs with her own pink shovel, inadvertently throwing dirt back into the hole I'm laboring over. She bends to pluck curious objects before they're lost to the settling soil. She scoops handfuls of dirt and rubs them over her skin like some luxurious moisturizer. And when we're done, she's the very best buried treasure a scurvy pirate dad could find.
* * *
"What's that yer working on?" he says.My daughter and I survey our territory, the moat and the castle mound that is currently a temporary home to assorted trucks, dolls, and transplanted flowering weeds.
"We're just digging!" shouts my daughter, waving her shovel triumphantly.
"Are you gonna plant something?" he asks uncertainly. "Tomatoes?"
"Not yet," I say, slowly looking around. "Just digging. For fun."
"Yeah! For fun!" shouts my daughter, emptying a cup of dirt over her head.
"Huh," he says, and turns away, shaking his head.Alan, our neighbor, is a gardening fiend. He can't wait until we stop foolin' about and start growing things. Which we fully intend. It's on our to-do list. Definitely in our 10-year plan. Living an hour from a reasonably-sized grocery store, it starts to make a lot of sense to grow things ourselves.
But for a little while, we're pretty okay with digging for the sake of digging. With examining roly-poly bugs and watching neat lines of ants trooping along the bottom of our trench. With laying on a pile of dirt and watching the clouds go by. With carving canyons with shovels and kitchen implements and with building tiny civilizations on the cliff edges. With getting so dirty that a shake of our clothes leaves a cloud of dust wafting through the air, and perpetually having dirt under our fingernails, a keepsake and reminder to get outside again soon.
The love of dirt is among the earliest of passions . . . mud pies gratify one of our first and best instincts. So long as we are dirty, we are pure.
-Charles Dudley Warner, My Summer in a Garden, 1870
Posted by neal call at 3:06 PM 6 commentsLinks to this post
Thursday, October 23, 2014
Feel the burn!
Last weekend I ran the Bakersfield 10K Volkslauf for my fifth straight year. I didn't really train much in the "preseason," since we're in the middle of trying to buy a house and, well, because laziness. I almost didn't show on race day, but finally dragged myself to the starting line for the sake of continuity. And because velociraptors. My daughter, enthusiastic about my athletic goals (or lack thereof), has taken it upon herself to help me get back in shape:
She's a tough taskmaster.
I used to try this same maneuver several years back when she was ten or fifteen pounds lighter, and I was ten or fifteen pounds more muscular. If I'm gonna be able to still manage it in several more years, I've obviously got to up my game. It's daunting, but dang-it, I'm gonna make it happen. When I think of a father-daughter obstacle racing team, I can already feel the pre-race butterflies flapping around excitedly in my stomach. Next year, at almost 6, she'll be the perfect age to start.
So, challenge accepted. I'm feeling the burn.
Posted by neal call at 8:38 AM 4 commentsLinks to this post
Friday, August 1, 2014
In your image, orange, the world was made
Lunchtime. I consider the oranges at the bottom of our refrigerator. A friendly neighbor unloaded them on us after a weekend of harvesting, and they practically spill from the clear plastic bin like plunder, like hoarded treasure. My four-year-old is happy to announce to friends and strangers met on daily walks that oranges prevent scurvy and that scurvy is something pirates and even regular people really ought to avoid. 'Cause it'll "make you dead." Unfortunately, my four-year-old is still asserting her claim to self-determination by refusing most food that comes in front of her, even that globe of citrusy goodness, the orange.
I take a gamble and begin peeling two oranges. She says "yuck!" and contorts her face in exaggerated expressions of disgust which escalate into melodramatic demonstrations of extreme pain. So I take a break and we go to the internet so I can read her some poetry about oranges. Kids dig poetry, right?
Neruda's persuasive words notwithstanding, my daughter will not be moved. "That's nice," she says. "But I still won't eat any orange." I sigh and finish peeling and then start eating.
I make my way through the segments of one orange, separating each crescent landmass from its companions, musing over the exploding flavor of sun and citrus in my mouth. I handle the textured bumps and protrusions of a piece of peel, and form a continent from it. I show my daughter where we live in North America. I make a Gulf of Mexico with my thumbnail. I sliver off a piece for Hawaii. As I carve out a piece for Central America my daughter absentmindedly grabs an orange segment and pops it in her mouth. I pretend not to notice. With the aid of a map from a National Geographic, I carve Canada and South America, Africa and Scandinavia, Australia and India.
The orange segments disappear, one by one, into her mouth, until all that remains is the shell of the world that once was, the outline of the multitudes it contained.
I make my way through the segments of one orange, separating each crescent landmass from its companions, musing over the exploding flavor of sun and citrus in my mouth. I handle the textured bumps and protrusions of a piece of peel, and form a continent from it. I show my daughter where we live in North America. I make a Gulf of Mexico with my thumbnail. I sliver off a piece for Hawaii. As I carve out a piece for Central America my daughter absentmindedly grabs an orange segment and pops it in her mouth. I pretend not to notice. With the aid of a map from a National Geographic, I carve Canada and South America, Africa and Scandinavia, Australia and India.
The orange segments disappear, one by one, into her mouth, until all that remains is the shell of the world that once was, the outline of the multitudes it contained.
With a spare lamp we become a sun and cross the face of the earth, waking it with the first rays of dawn and letting it fall into a slumbering darkness.
Each orange consumed offers a brief overwhelming shot of both bitter and sweet, one besting the other but never eliminating it.
Inseparable the two tastes exist, one the greater and one the lesser. And all packed together into the ever diminishing, juicy heart of the orange.
Posted by neal call at 9:22 AM 25 commentsLinks to this post
Tuesday, July 22, 2014
In the middle of the night, my four-year-old knelt down by my bed, tapped me on the shoulder, and whispered into my ear.
Her: "Daddy, I'm scared. It's so dark."
Me, (trying to remember where I am and whispering so as not to wake my wife): "Um. Okay. Let's, uh, go back to your bed and I'll sing you some songs."
Her: "Okay."Once she's back in bed, I lay down next to her and we sing quiet duets of "Row, row, row your boat," and "Twinkle twinkle little star," and Diana Ross' "If we hold on together."
I pause a moment as I feel a spider crawl across my bare foot. I flick it away from me.
My daughter: "Daddy! Was that a spider!?"
Me: "Yes. But don't worry, I got rid of it."
Her: "But it looked like a baby! You don't hurt baby spiders. You take care of them (this seems to be a pattern)! Baby spiders are my favorite!"I sigh. A minute later, we're both on our hands on knees looking for the baby spider, and upon finding it, my daughter marches me to the front door where I deposit it gently outside.
My daughter: "I'm not scared anymore. Goodnight, dad."
Me: "Goodnight. I love you."
Her: "I love you too. And I love baby spiders."
Me: "Okay."I finally crawl back into bed, and all I can dream about it eight-legged crawly things. I'm pretty sure my daughter and I are sharing dreams.
And when I wake up and pull aside my covers, I kid you not, a spider (very much not a baby) scampers out of my bed.
It's a little frightening the way a kid's dreams, if nurtured, become reality.
The next day, we had more spider conversations on the walk home from pre-school:
Her: "Dad, do spiders talk?"
Me: "Probably. Or, at least they use body language."
Her: "Hmm. Well, I want to look up on the internet how to talk spider language. 'Cause I want to ask a spider to be my pet. Lately they just keep running away from me."Keep on running spiders. Keep on running.
Posted by neal call at 10:18 AM 5 commentsLinks to this post
Sunday, June 15, 2014
A father for the fatherless
Neal here. This is Lindsay's very first guest post on my blog. I held her off for as long as I could, but after two years of blogging, she finally wore down my defenses. Which, coincidentally, is also the narrative arc of our courtship and marriage. Ha! I kid (not really, it's actually pretty accurate). She's a great writer, and is in fact the one who first encouraged me to start writing personal essays. Is it weird that one of the ways my wife and I connect is to exchange essays and then edit together in knock-down, drag-out sessions of metaphorical fisticuffs? I'm sure it's normal. Anyway, right here's an example of why I decided to marry her; this girl's got a soul in her, and it's hard not to want to be a better person when you're with her. I wrote a piece around Father's Day last year that detailed my experiences assisting my wife's research with a different vulnerable population. I'm proud of my wife, and I'm proud to showcase this piece, 'cause it's a powerful reminder that no matter your position in life, you gotta fight to make a difference, and that however distant redemption seems, it never disappears.
There is barely an inch of uncovered space in the Resource Center where I work. Flyers advertising school lunch programs and community health screenings obscure the glass window in the front door. Clipboards of blue, tan, and salmon colored papers greet clients as they enter. Someone asks if I have any scholarship applications. I sift through a thick manila folder. How about a bus schedule? I pull one off the tack board hanging above my desk. Can I get a dog license? Sure, just fill out this form. Diapers? It's an emergency! I grab my key to the supply closet. I want to quit smoking, can you help? I lead them to a magazine stand labeled Smoking Cessation Programs. I'm worried that my son was molested. First, let me close the door. Now, tell me everything, as I pull out a Suspected Child Abuse Report. At the Resource Center, we don't tell people what we do. We ask them what they need because, chances are, we can help.
But the help isn't free. The currency we exchange is paperwork. I greet each client, shake hands, show them into my office, and proceed to complete forms with titles like:
The titles sound so sterile, so clinical. Just the core data elements, please. Nothing superfluous. Tell us a story? God forbid.
But it turns out that stories are what 68-year-old Italian men do. I knew right off Marty wasn't from around here. With my office just across from the receptionist's desk, I hear clients before I see them, and a Brooklyn accent sticks out like a sore thumb in a place that's nearly 3000 miles away on the opposite coast.
In my three months of working at the Resource Center, I've noticed that the desperation the client feels is directly proportional to the number of people they tell their story to as they enter the front office. While I finish up my previous client's paperwork, I hear Marty's voice moving around the room, barely a pause to draw breath. To the receptionist, he details the unexpected drug bust on his niece, which led to the heart attack, which led to the month-long hospitalization. To the quiet client waiting for her appointment, he exclaims, "I had no idea my niece was into that stuff, you know! It's crazy!" To the Sparkletts delivery man, collecting our empty bottles, Marty explains, "It's been a month since I seen my Godson -- that's as long as we been apart his whole life."
Sometimes it takes a while to understand just what a client is asking for. It's understandable; when life is spinning out of control, it can be hard to figure out what will stabilize it. But as he sat down in my office, Marty told me exactly what he was there for.
"My Godson was taken away on account a his mom doin' drugs -- they found needles and all kinds of crazy stuff in her room -- and I need to get 'im back. I need the social worker to fax me the paperwork here and then I need you to help me fill it out, make sure I'm doin' it right cause he's been away from me for over a month on account a me goin' to the hospital for my heart attack. The shock's what did it. The police come busting into my house -- and I'm retired law enforcement, mind you -- and search all the rooms. They haul Barb right off and I can't believe the things they're pullin' out of her room. I had no idea. And while I'm in the hospital just tryin' to survive, Barb ups and signs away her rights. So the state's got Mike now but I gotta get him back. Ya see what I mean?"
It wasn't long before I had the paperwork in hand.
Verification of Relative or Non-Relative Extended Family Member (NREFM)
Application for Assessment of Relative/NREFM Home
Just three short pages, about 36 questions, to explain why you should be the one to love and care for a child. I hand the papers to Marty but he puts his hand up to block them. "See, I need you to do it," Marty says, a hint of pleading in his mostly matter-of-fact tone. "We gotta get everything just right. Will you write for me?"
No problem, I think. Paperwork is what I do. I read aloud the first question and set to work squeezing his narrative into the space provided.
"It's today. He turns 13 today. 3/22/2001. I can't believe I don't getta be with 'im on his birthday. I talked to the social worker and said I gotta speak with 'im. They let me call 'im but when I heard his voice I just broke down. I couldn't even talk. I just bawled. I can't believe I'm not with 'im for his birthday, he's thir . . . ."
He breaks off in quiet sobs. I look up from the paperwork and instinctively stretch my hand toward him. But I stop halfway there, resting it on the file cabinet that separates us. It's still at least a foot from being anywhere near to a consoling touch. After a decade working with prisoners, who were shackled to avoid physical contact, I'm still grappling with how to reach out.
I stare at the paper. I have four and a half lines to distill from Marty the special ways he comforted his infant Godson; how he had to bend his 6' 7" frame in half as he held the hand of a toddler learning to walk; how he walked the preschooler to classes everyday because "he has some learning problems but he's hangin' in there"; how he drove the teenager to and from high school right up until the day the cops burst in.
"He's been my whole world, you know," Marty concludes. I wonder if that description is brief enough for the powers-that-be.
"Oh, yeah. I'd take care a 'im forever. I saw my niece. I says, 'Barb, why'd you sign over your rights so fast? You don't give up on your own baby.' She said, 'So I have to hear this from you?' Damn right, you do! You don't give up your kid for drugs. I don't care if they're 2 or 62, they're your baby forever. How does she not get that? I'll take care a 'im forever."
I'm blinking back tears. That's another thing I haven't figured out yet: do I let them see me cry? It's hard to do paperwork through welled-up tears, and drips will smear the ink. Do I need to look unphased, to instill confidence that things will be okay? Or can I show them that I'm a little afraid that the thing they want, more than they've ever wanted anything, might never be? Just released from the hospital, disabled, living with a friend, not realizing drugs were infiltrating his home until the cops came knocking. I'm no custody expert, but these don't sound like good signs.
One and a half lines, this time. Barely enough room to say, "Please, I'll do anything to be with my Mikey again"; no room to post your whole heart on the page.
We finish up with his signature and I prepare the fax. The space between the faxing and the confirmation feels interminable, his and Mikey's whole future hanging in the balance. I assure him that I'll call the social worker to make sure she received the fax. I know she did, and he doesn't ask me to call, but it's all I can do and so I'll do it. As he gets up to leave, he tells me matter-of-factly, "I'm gonna hug you." He wraps his hulking arms around me -- I'm not sure I've ever been held in such long arms and I can feel how powerful they still are -- and whispers, "You're an angel," as he kisses my cheek with a loud and purposeful muah. He turns to leave and I still haven't moved. Should I have hugged back? What were my parting words? Did he see my smile? I can still hear him repeating thank you, thank you, thank you as he hobbles out of the office, and I can't stop smiling. Finally, I take a shaky breath and sit down to finish my paperwork.
Posted by neal call at 1:39 PM 18 commentsLinks to this post
Thursday, June 5, 2014
What to do when you find a black widow
When we moved up into the mountains nine months ago, we knew that there would be an increase in certain dangers over our suburban environment. We nixed the first rental I fell in love with after the real estate agent warned that its location up on the edge of the mountain would attract rattlesnakes and that bobcats and mountain lions might think our daughter looked tasty.
Now that we live a little further down the mountain, and aside from the mild surprise of bear cubs living under our deck and our neighbor who feeds a community of raccoons from his front porch, our run-ins with wildlife have been pretty benign. The stray wind-scorpion has been enough to keep us pleasantly on our toes.
But in the last few months, I've noticed webs and what appear to be egg sacs in the corners of our garage. De-cluttering the space and cleaning up the webs went on my list of things to do, a list which might also be called my "list of things I probably won't do anytime soon."
That is, until the evening this week that I found this beauty hanging out next to our washing machine:
Yeah, that's a black widow. You can tell because when you see it, your heart stops beating for a second, you break out in sweats, and you have to fight the urge to run screaming. Individually, these symptoms might not be conclusive, but when you put them all together: yep, it's a widow. Here it is again, with a banana for scale:
After stepping away and calming myself, I found a jar and leather gloves and collected the evil-looking specimen. My wife found me some time later, and curiously watched as I repeatedly picked up the jar to examine it closely and then lost my nerve, put the jar back down, and stepped back several paces to morosely consider my family's imminent demise.
"Whatcha got there?" my wife asked. "A black widow," I said, feeling like I was pronouncing a death sentence. "I think there's more of 'em out there, too." I tried not to think about how numerous and tiny spider babies are. (I'm still trying not to think about it.)
"Oh, we used to have those all the time in our shed growing up. Just try not to touch 'em." she said. "Anyway, did you finish the laundry?"I tried to tell her that it was while doing laundry that I'd found this minion of Satan, this death-dealer, and that it was unlikely I'd ever be able to do laundry again. But she raised her eyebrow and told me not to be a baby. And to finish the laundry.
I debated flushing the widow after I'd gotten a few good pictures as proof of my near-death experience. But then I decided that if we're in widow country, I'd better take this opportunity to educate my daughter about things that are dangerous and that we never, never touch. Had I tried this talk with her a year or two ago, it probably would have backfired. Telling a toddler not to touch something pretty well guarantees it. But this four-year-old of mine, she's really growing up. The best I can do is teach her right and then give her opportunities to demonstrate that her instincts for self-preservation are finally kicking in.
Of course, when I showed her the spider the next morning, I probably could have predicted her reaction:
Her: "Oh, wow! I just love it so much!"
Me: "Did you understand when I said that it was dangerous?
Her: "Yes, but it's so beautiful! I love the red on its body. That's a pretty color!"
Me: "If it bites you, you might die."
Her: "I want to name it Noodle! Can I keep it?!"Sigh. I finally extracted a promise from her that if she found any similar spiders, she wouldn't touch them. But she didn't promise that she wouldn't name them or love them. I can't help feeling that there's some validity to her attitude; it's just hard as a dad to reserve any appreciation for hazardous things, things that threaten the well-being of your kid.
But that's life, I guess, right? The black widow may as well be a metaphor for the risks that we'll all encounter. Life: it's beautiful, and it's dangerous.
Even more, the black widow is a metaphor for raising kids. When my daughter was a newborn, a black widow's bite would have been horrific. In the first year or so, it seemed everything about this infant's environment was a threat. And since babies have very bad judgment, it was almost silly how many of those threats could be lethal. Grapes, grapes were our greatest fear.
But at four years old, she's making big strides in discernment. Many of the things that were so dangerous when she was an infant are not nearly so dangerous now. If a widow bit her, it'd still be bad. But there's very little chance it would be lethal. As with so many other risks that we've feared, the danger of the widow decreases little bit by bit with each year. The risks aren't negligible; but they somehow become manageable enough that after safely reaching adulthood ourselves, we parents actually decide to bring more fragile souls into this dangerous world. The idea is that we'll be able to shield them just long enough for them to stop putting bugs in their mouths and knives in electrical sockets, and if we've done our job, they'll gradually be able to negotiate future dangers without needing our intervention.
In the meantime, yeah, I'm gonna be cleaning out our garage. But no matter how much I clean, there will still be black widows in the world, and I'm gonna try not to freak out, and instead take this moment to practice trust - trust that my growing girl can start negotiating some of the world's dangers for herself.
UPDATE: In the two days since we found our widow, my four-year-old and I have talked a lot about Noodle and what makes her special. And frightening. And lovable. If we're sitting at my computer, she'll say, "type in 'spider'! I want to see more cute spiders!" or she'll say, "how do you spell 'spinneret'? NO, LET ME TYPE THE LETTERS!" And of course there's now all sorts of creepy-crawly bug art floating around the place now. Which is kind of cool. After she went to bed, I finished doodling out the spider diagrams I started with her to explain spider anatomy:
And yesterday, in the thick of all of our arachnid fever, we decided to have her visit the Insect Lore Bugseum in Bakersfield, after which she brought home her very own Praying Mantis egg case (bought buy her Tio), also called an "ootheca." Which, I assure you, is quite amusing to hear a four-year-old pronounce.
I don't always tame my fear and capture a black widow and research the bejeezus out of it and plan appropriate safety and science lectures for my kid, but when I do, it sure can be a lot of fun.
Posted by neal call at 1:41 PM 5 commentsLinks to this post
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The Many Reasons of Success
- Details
- Category: Quotes & Speeches
- Published on 14 Mar 2012
"When I started here, I only thought of our survival. We had to work so we could get our food for free, for the kids to have baon for school. My only vision was to have a restaurant with air conditioning. Thanks to our customers, we now have four."
-- Julita Urbina, matriarch and CEO of the family corporation which runs Cafe Laguna, one of the more successful restaurant names in Cebu City.
In her Entrepreneur magazine interview, she mentioned that with a budget of P300, she started serving Filipino food in a carinderia in front of the Cebu military camp where the family patriarch, who hails from Laguna, was stationed. From a carinderia called "Mother's Best" in 1979, it transformed into Cafe Laguna in 1991. Cafe Laguna grew big on quality and good food.
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text:
Sweet Orange Stir Fry
- prep time 1 min
- total time 1 min
- serves 4
This is a wonderful light dessert when you need a touch of something sweet after dinner. It's also nice over vanilla ice cream, mango sorbet or rice pudding. Instead of using naval oranges, use mandarin oranges if you like
Christine Cushing
Chinese, Dairy-Free, Chinese New Year, Vegan, Party Favourites, Stir-Fry, Sauté, Dessert, Fruit