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Hello, Steven, it's nice to meet another cousin. By the look of your names, I can't help you. I don't have any Sydneys anywhere at any time. My Frostick males are James, William, John, Thomas, Charles and the like. Sorry.
Regards, Pam, Australia
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After a great deal of thought, I am today announcing that I will be taking a break from speedway and will not be racing at all during the 2015 speedway season.
This last season has been a struggle for me both personally and professionally, I have lost my love for the sport, have no desire to race and am quite simply not enjoying it anymore. To be a successful rider you have to make many sacrifices and a lot of these have a major impact on your life away from speedway. When you get pleasure from your racing, these sacrifices are easier to make, but when you are not then it is a very different situation. Speedway is a dangerous sport and when you're not enjoying it, there are safer ways to make a living. It is time for me to do something else.
I would like to thank everyone at Lakeside and ZKS ROW Rybnik, particularly the supporters of both clubs who have been fantastic in their love and support of me. I would also like to thank my sponsors who believed in me, my promoters, my off track team and most especially my grandfather Alan Thompson, all of whom have helped me along the way.
It is my hope that I will benefit from taking a break from speedway, that I will re-discover my love for the sport and perhaps I will return better and stronger in the future.
Lewis #27


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text: Local Shows: Killer Shows @ The Dark Horse By JC April 2, 2012 12:59 PM Share on Facebook Share on Twitter Photo courtesy of Getty Images Slow week for shows on the local side but the few that we have are well worth going to! Both of them are at the Dark Horse this Thurday and Friday. Pull on your party pants and join me for a two day concert session! Thursday, April 5 Dark Horse 9pm | 21+ Sammus Theory Letters to Luci All Things Pertaining Friday, April 6 The Dark Horse 9pm | 21+ Continental (featuring ex members of the Dropkick Murphys) Monkey and a Roast Beef Sandwich I know I'll be there for both, will you? Filed Under: continental, dark horse, local 406, local bands, sammus theory Category: Blogs, Concerts, Events, J.C., Music Share on Facebook Share on Twitter More Leave a comment Send to a friend! Print this page Pin it! Reddit This! Share on Tumblr! More From 96.3 The Blaze Weekend Bender November 22 'Sonic Highways' Goes to New Orleans Montana Hunting and Fishing Update 11/22/14 [AUDIO] Motel Robber Appears in Court – Witness said Suspect 'A Meth Addict Desperate For Money' [YouTube] Best of the Web


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WOW WOW WOW...is all I can say without being speechless about this website. I have been performing magic for about ten years now, and this is the...Read More ->
Being outside the US, I have tried many online magic shops (no brick & mortars in my area). In the past I've had packages gone missing, wrong...Read More ->


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Photo Credit: ABC
The woman who has already sparked a scandal on the new season of ABC's The Bachelor has been identified. According to RadarOnline.com, the contestant who allegedly had a relationship with a producer on the reality show is model/actress Rozlyn Papa.
Though Papa was supposed to be interested in winning the heart of the new "Bachelor," pilot Jake Pavelka, she reportedly caught the eye of an ABC employee behind the scenes. Contrary to rumors, she denies the relationship was intimate: "No, I did not have a sexual relationship with a producer on the show," she told the Web site.
The 28-year-old single mother, who reportedly figures prominently in Monday's episode when the relationship is uncovered, says she and the crew member are no longer an item.
"I had a relationship with someone on the show that didn't benefit them," she told the Web site. "We remained really close but we are not dating now."
Papa also has a problem with the show hyping her behavior as "inappropriate."
"What they mean by 'inappropriate relationship' is not what 'inappropriate relationship' means in the real world," she said. "This is a TV show and it's made for entertainment, but it is a far cry from reality," she said. "I know that things on television weren't always as they seemed, but I had no idea the extent of the show manipulation."
Bachelor host Chris Harrison spoke candidly about the situation during an interview earlier this week. "It was incredibly unfortunate. Horrible decisions were made," he said. "It was embarrassing for us and in fairness to Jake and for the other women, [we said], 'Let's nip this in the bud and take care of it.' And that's what we did."
And as much as reality TV competitions bank on unexpected drama, Harrison says the incident left a bad taste in everyone's mouth.
"There's train wrecks in this show where I'm like, 'Oh, it's going to be great. Let's let them have it.' But," he added, "I felt bad for everybody, and it was an uncomfortable deal."
Plus: The Search for the Perfect Mate Continues on The Bachelor
Do you hope Rozlyn Papa is kicked off The Bachelor for her behavior? Chime in below!


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Most of us understand how to sew on a button. If not, we have tutorials on sewing them on by hand as well as by machine. Pretty darn easy either way, and not scary at all. But buttonholes are a whole different matter. At the end of your project, after you've put in so much work, it's time to put in the buttonholes. You should be happy you're almost done. But for many of us, beads of sweat start to form across our brows and we wonder, "Am I about to ruin everything by botching the buttonholes?" Well, you can stop sweating, because it's really not that hard once you break it down into individual steps.
Travel Tote with Two-Tier Wraparound Pockets
When you're on the road, doesn't it always seems to be the ordinary, everyday things you are suddenly in desperate need of: gum, nail clippers, your headphones? I get a little panicky, certain I've left behind the most obvious items... as if perhaps I'm on an arctic expedition and won't see civilization for weeks. But the opportunity for replenishment doesn't matter; I want my stuff close at hand. That's the theory behind our handsome travel tote: plenty o' pockets to stash all your stuff. There are four big outside pockets that wrap front and back plus a deep inside compartment (exactly deep enough for a magazine, I might add!) with its own generous interior pocket. I'm still likely to forget something, which is why after my current travels, I now own six pairs of sunglasses and a dozen tubes of lip balm.
Keepsake T-Shirt Pillow with Knotted Corners
We all have them: those "special" T-shirts from events, vacations or sports teams. Kids have more of them than most! You don't wear them anymore, but you also can't seem to part with them. Let's make them into some cute keepsake pillows! Carefully cut out the motif, stitch and stuff! It really is as easy as that. We created a pattern for cool knotted corners that can be done in the same or a contrasting color (a way to get rid of yet another shirt). One or more of these pillows would be a great gift for someone heading off to college: a little bit of home on which to lay your head.
Picnic Placemats with Flatware Pockets
Outdoor dining takes a little creativity in order to keep everything from blowin' in the wind. Our darling, durable placemats have built-in pockets to hold flatware and/or napkins. Dining in? Hooray for the reversible! Simply flip over the placemats for whole new look – the back is pocket-free. These clever placemats are so quick and easy, you can easily whip up enough for your own table or the entire family reunion in a single weekend. You want a substantial fabric for this project: a canvas, heavy cotton duck or an outdoor fabric. We went the outdoor fabric route, which worked well and looked great. We love the bright, bold designs common in the outdoor category – perfect for casual summer meals.
Janome Monday: Embellished T-Shirts
Now's the time to stock up on cute summer tees. They're on sale nearly everywhere. We hit the local Target and Old Navy in our area and didn't spend over $6.99 for any of our shirt samples. Okay, so now you have a pile of T-shirts. Step #2 is to personalize them. It's Janome Monday, and our goal today is to show you how fun and easy it is to use your sewing machine to turn standard off-the-rack items into something special. We pulled together a handful of fave fabric scraps to add pockets to some of the shirts and a few lengths of gorgeous lace to alter the others with inset panels and cropping. Fast fashion update!
Flat Bench Cushion with Piping and Buttons
A bench cushion is one of the top projects new sewers list as the decorating puzzle that finally got them thinking about making something on their own rather than buying. Standard bench cushions are certainly available in stores, but I think we can all agree that they never, ever, ever fit right. The piping and buttons are optional, but they add the professional finishing touches that make all the difference... and impress all those who will soon be sitting on your new bench cushion.
Empire Waist Apron in Wonderful Waverly Fabrics
We step back into the wonderful world of Waverly with this lovely empire waist apron. You've likely seen the Waverly brand on all kinds of home decor items, from window treatments to storage to bed linens. But their original product line was fabric. Starting in 1923, Waverly has produced some of the most beautiful and long-standing collections of any manufacturer. We've broken out of the standard home décor box of cushions and coverings with an apron design, which uses two Waverly medium weight, 100% cotton twills: Paisley Prism and View Finder from the Latte colorway.
Insulated Baby Bottle Carrier
Babies and their entourages (sometimes referred to as parents) are always on the go: to the store, the park, the playgroup, the zoo, anywhere and everywhere. When they're out and about, they like to have their favorite drink nearby. Mom gets her 'Bucks venti half white mocha, half cafe vanilla, ez ice, 2 shots pour appigato style with whip and caramel drizzle frappachino (actual order overheard at one of our local Starbucks®)... baby gets his/her bottle - nicely warmed or chilled, thank you very much. It's an easy request to fill thanks to our baby-on-the-go thermal baby bottle carrier. There's a water resistant PUL lining, a layer of insulating batting, and a super trendy fabric exterior with a handy Velcro® strap.
Janome Monday: Appliquéd Kite Tote
Let's go fly a kite! This tote is ready to take off on your next adventure or at least on your next excursion to the store. The shopper style construction is fast and easy with deep 4" boxed corners and no-sew webbing handles. Let your creativity soar with the fun front appliqué. We offer a template download for the kite's body and tail. And as a Janome Monday project, we show you two different presser feet that make quick work of this eye-catching embellishment.
What Is Fabric Grain And How To Fix It If It's Off
You might have heard the term, "fabric grain." It sounds like it could be a breakfast cereal just for sewists. But in reality, it's a technical term that describes the direction your fabric has been woven. It's important to know which way the grain is running. Because, fabric that is off-grain when you are cutting pattern pieces can cause your completed project to stretch out of shape. We're here to give you a better understanding of fabric grain and some tips on how to straighten it.


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Keeping the garden tidy requires a few deft moves with the right tools, and, time and again over the seasons, shrub rakes are... Read more »
Entire books have been written on the science of making compost, but it isn't as hard as people think. In five easy steps, you... Read more »
Weeding, pruning, and raking all make a huge difference in the appearance of a garden, but, to finish the job, you have to rou... Read more »
The Fiskars® aluminum shrub rake features a slim head with uniquely tapered tines that are perfect for reaching into tight spac... Read more »
Our Eco Bin Composter features an easy-to-assemble, easy-to-use design that can simplify and speed the composting process. It i... Read more »
Our HardShell® Kangaroo® Gardening Container is perfect for all your outdoor cleanup needs — whether you're gathering yard and... Read more »
Are school fundraiser ideas keeping you up at night? A unique handmade art piece that represents your school is sure to be a p... Read more »
Creating beautiful and personal touches does not have to be difficult, especially when you have great designs to work with! Read more »
Recycle and give a new life to some of your old T-shirts Read more »
Teresa Collins is a top craft celebrity who has been featured numerous times on My Craft Channel, HSN, QVC and DIY network, wel... Read more »
Teresa Collins is a top craft celebrity who has been featured numerous times on My Craft Channel, HSN, QVC and DIY network, wel... Read more »
Our unique Tag Maker with Built-in Eyelet Setter features an innovative design that makes it easy to create tags perfect for gi... Read more »
By creating a few simple tags, you won't be caught at the fabric store not knowing what fabrics or yardage you have in your st... Read more »
A brocade drawstring pouch can be a beautiful and luxurious accessory or gift. Read more »
Transform a simple hoodie into a super simple unicorn costume and take the stress and pressure out of making a complicated Hal... Read more »
Perfect for a wide range of sewing and quilting tasks, our Amplify® RazorEdge™ Serrated Fabric Shears sense blade separation an... Read more »
Perfect for a wide range of sewing and quilting tasks, our Amplify® RazorEdge™ Fabric Shears sense blade separation and force t... Read more »
Perfect for a wide range of crafting and mixed media tasks, our Amplify® Mixed Media Shears sense blade separation and force th... Read more »
Are school fundraiser ideas keeping you up at night? A unique handmade art piece that represents your school is sure to be a p... Read more »
Try some new punches out and make some cards to celebrate World Card Making Day! Read more »
A personalized Duck Tape® crown is quick and easy to make with your Fiskars® Duck® Edition Scissors. It is a fun way to cele... Read more »
Our Preschool Training Scissors features a special training lever that opens the blades after each cut, helping children learn... Read more »
Children love our Designer Non-stick Blunt-tip Kids Scissors for the colorful handle patterns that make cutting fun and the non... Read more »
Our Designer Non-stick Student Scissors are larger than our Kids Scissors but smaller than adult scissors, perfect for those ol... Read more »
Transform a basic jacket into something personal and unique. Read more »
Create a simple reusable calendar to plan all of your back to school activities. Read more »
Creating a miniature collage with your Fiskars® Duck® Edition Scissors is a great way to use up any last bits of Duck Tape® yo... Read more »
Designed for long, easy cuts down strips of Duck® Tape, our Duck® Edition Scissors feature a non-stick blade coating that preve... Read more »
Designed for all-purpose cutting through a range of craft materials that incorporate glue, tape and other sticky adhesives, our... Read more »
Designed for tight, precise cuts through a range of craft materials that incorporate glue, tape and other sticky adhesives, our... Read more »
Choose a Die and Letterpress Plate
Place in Cover
Choose a letterpress plate and place it in the coordinating cutting die.
Place orange cover underneath cutting die. Built-in pins raise the letterpress plate, allowing it to be inked without getting ink on the face of the die.
Ink the Plate
Color in the raised letterpress plate design using standard fabric-topped ink pads or markers. Holding the ink pad on the design and applying a circular motion will ensure complete, even coverage.
Lift the cutting die with inked letterpress plate out of the orange cover. Without the cover, the letterpress plate will nest back into the die.
Make a Sandwich
One Pass Through
Place crafting material on top of inked letterpress plate. The letterpress plate rests slightly below the face of the die, so the placement of your paper can be moved without getting unwanted ink smudges.
With one hand, gently push the "sandwich" toward the roller. With other hand, turn the crank. Once "sandwich" is connecting with the rollers, it will move through powered by the hand crank.
Final Step
Professional Result
Once the "sandwich" has completely run through the rollers, the cutting plate can be removed and the result can be accessed. If the finished result is difficult to take off the letterpress plate, place the orange cover under the die — the letterpress plate and finished result will raise and be easier to access.
Your perfectly aligned, die-cut and letterpressed embellishment is complete. Use it to accent a homemade card, scrapbook page or just about any other paper craft project.
Sign up today for project inspiration, tips, techniques and product specials.
© Fiskars Brands, Inc.
Orange-handled scissors are a registered trademark of Fiskars Corporation.


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Item #: ES78584392052
Mfg. Part #: LM15662
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Item Description:
Product Details: Lincoln Electric Motor LM15662 -- 1 Each
Manufacturer: Lincoln Electric Motor
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Product Specifications:
AC Variable Speed: Motor Type - TEFC Totally Enclosed Fan Cooled
Horsepower - 20
Volts/Phase - 230/460, 3PH
Frame Size - 256T
Material - Construction - Extruded Aluminum Frame, Cast Iron Brackets
Efficiency - 91.00 Percent
Service Factor - 1.25
Hertz - 60
Insulation - Class F
Mounting - Foot Mount
Weight - 236 lb
Manufacturer Model - AF4P20T61
Manufacturer Series - Ultimate E
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To be sold at the Kissimmee 2015 auction, Kissimmee, FL, January 16-25, 2015. To consign a car or to register to bid, visit the... More
- Detailed restoration- Rare factory Black with Black interior- 1 of 154 with this paint/trim combination- 428 CI Cobra Jet engine- Factory 4-speed- GT... More
- Gold Metallic with White sill stripes- Black Deluxe interior- Console compartment signed by Carroll Shelby- 428/360 HP Police Interceptor V-8- Aluminum intake and... More
Top down thrills with Ford big block performance Since the new-for-1967 Mustang body shell had been designed from the outset to accept Fords larger... More
1968 GT 500 This is a factory candy apple red four speed. It also appears the that air condition was added early in it's life... More
**Available at MECUM Auction Kissimmee Floriday January 23rd in the Catalogue Sale** 8T02S143365-01366 You are looking at an extremely nice example of an original 1968 Shelby... More
1968 Shelby GT 500 Factory acapulco blue 4 speed that has fresh paint. This GT 500 runs and drives great and is a solid rust-free Shelby... More
1968 Shelby GT 500 Convertible This GT 500 convertible is one of 402 Shelby's that were offered in 1968 with a 428 PI. It's been... More
SHELBY: 1968 GT500 convertible, lime gold ext, saddle int, 428, auto trans, ps, pb, factory a/c, 10-spoke wheels, fully documented original Shelby, very rare... More


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Caenorhabditis elegans teneurin, ten-1, is required for gonadal and pharyngeal basement membrane integrity and acts redundantly with integrin ina-1 and dystroglycan dgn-1.
ABSTRACT The Caenorhabditis elegans teneurin ortholog, ten-1, plays an important role in gonad and pharynx development. We found that lack of TEN-1 does not affect germline proliferation but leads to local basement membrane deficiency and early gonad disruption. Teneurin is expressed in the somatic precursor cells of the gonad that appear to be crucial for gonad epithelialization and basement membrane integrity. Ten-1 null mutants also arrest as L1 larvae with malformed pharynges and disorganized pharyngeal basement membranes. The pleiotropic phenotype of ten-1 mutant worms is similar to defects found in basement membrane receptor mutants ina-1 and dgn-1 as well as in the mutants of the extracellular matrix component laminin, epi-1. We show that the ten-1 mutation is synthetic lethal with mutations of genes encoding basement membrane components and receptors due to pharyngeal or hypodermal defects. This indicates that TEN-1 could act redundantly with integrin INA-1, dystroglycan DGN-1, and laminin EPI-1 in C. elegans development. Moreover, ten-1 deletion sensitizes worms to loss of nidogen nid-1 causing a pharynx unattached phenotype in ten-1;nid-1 double mutants. We conclude that TEN-1 is important for basement membrane maintenance and/or adhesion in particular organs and affects the function of somatic gonad precursor cells.
Article: C-terminal region of teneurin-1 co-localizes with the dystroglycan complex in adult mouse testes and regulates testicular size and testosterone production.[Show abstract] [Hide abstract]
ABSTRACT: Testicular size is directly proportional to fertility potential and is dependent on the integration of developmental proteins, trophic factors, and sex steroids. The teneurins are transmembrane glycoproteins that function as signaling and cell adhesion molecules in the establishment and maintenance of the somatic gonad, gametogenesis, and basement membrane. Moreover, teneurins are thought to function redundantly to the extracellular matrix protein, dystroglycan. Encoded on the last exon of the teneurin genes is a family of bioactive peptides termed the teneurin C-terminal-associated peptides (TCAPs). One of these peptides, TCAP-1, functionally interacts with β-dystroglycan to act as a neuromodulatory peptide with trophic characteristics independent from the teneurins. However, little is known about the localization and relationship between the teneurin-TCAP-1 system and the dystroglycans in the gonad. In the adult mouse testis, immunoreactive TCAP-1 was localized to spermatogonia and spermatocytes and co-localized with β-dystroglycan. However, teneurin-1 was localized to the peritubular myoid cell layer of seminiferous tubules and tubules within the epididymis, and co-localized with α-dystroglycan and α-smooth muscle actin. TCAP-1-binding sites were identified in the germ cell layers and adluminal compartment of the seminiferous tubules, and epithelial cells of the epididymis. In vivo, TCAP-1 administration to adult mice for 9 days increased testicular size, seminiferous and epididymal tubule short-diameter and elevated testosterone levels. TCAP-1-treated mice also showed increased TCAP-1 immunoreactivity in the caput and corpa epididymis. Our data provide novel evidence of TCAP-1 localization in the testes that is distinct from teneurin-1, but is integrated through an association with the dystroglycan complex.Histochemie 10/2013; · 2.61 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Using a chemoproteomic strategy, we recently demonstrated the expression of teneurin-2, a transmembrane glycoprotein, in the majority of malignant mesothelioma cell lines. This finding was unexpected since no formally organized evidence existed to implicate teneurins in human malignancy. For this reason, here we provide a comprehensive review on the expression of teneurins in human tumors and cell lines. Current evidence supports the aberrant expression of teneurins in various tumor types, their involvement in cancer-related regulatory networks, and their potential participation in drug resistance. Structural attributes of teneurins could enable the detection of shedded forms in body fluids for clinical applications.Cancer letters 07/2012; 326(1):1-7. · 5.02 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The Drosophila model represents an attractive system in which to study the functional contribution of specific genes to organ development. Within the embryo, the heart tube serves as an informative developmental paradigm to analyze functional aspects of matricellular proteins. Here, we describe two essential extracellular matricellular proteins, Multiplexin (Mp) and Lonely heart (Loh). Each of these proteins contributes to the development and morphogenesis of the heart tube by regulating the activity/localization of essential extracellular proteins. Mp, which is secreted by heart cardioblasts and is specifically distributed in the lumen of the heart tube, binds to the signaling protein Slit, and facilitates its local signaling at the heart's luminal domain. Loh is an ADAMTS-like protein, which serves as an adapter protein to Pericardin (a collagen-like protein), promoting its specific localization at the abluminal domain of the heart tube. We also introduce the Drosophila orthologues of matricellular proteins present in mammals, including Thrombospondin, and SPARC, and discuss a possible role for Teneurins (Ten-A and Ten-M) in the heart. Understanding the role of these proteins provides a novel developmental perspective into the functional contribution of matricellular proteins to organ development.Matrix biology: journal of the International Society for Matrix Biology 04/2014; · 3.56 Impact Factor
Page 1
Molecular Biology of the Cell
Vol. 19, 3898–3908, September 2008
Caenorhabditis elegans Teneurin, ten-1, Is Required for
Gonadal and Pharyngeal Basement Membrane Integrity
and Acts Redundantly with Integrin ina-1
and Dystroglycan dgn-1
Agnieszka Trzebiatowska,* Ulrike Topf,* Ursula Sauder,†Krzysztof Drabikowski,*
and Ruth Chiquet-Ehrismann*
*Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, CH-4058 Basel,
Switzerland; and†Microscopy Center, Pharmazentrum, University of Basel, CH-4056 Basel, Switzerland
Submitted January 14, 2008; Revised June 27, 2008; Accepted July 1, 2008
Monitoring Editor: Jean E. Schwarzbauer
The Caenorhabditis elegans teneurin ortholog, ten-1, plays an important role in gonad and pharynx development. We
found that lack of TEN-1 does not affect germline proliferation but leads to local basement membrane deficiency and early
gonad disruption. Teneurin is expressed in the somatic precursor cells of the gonad that appear to be crucial for gonad
epithelialization and basement membrane integrity. Ten-1 null mutants also arrest as L1 larvae with malformed pharynges
and disorganized pharyngeal basement membranes. The pleiotropic phenotype of ten-1 mutant worms is similar to
defects found in basement membrane receptor mutants ina-1 and dgn-1 as well as in the mutants of the extracellular
matrix component laminin, epi-1. We show that the ten-1 mutation is synthetic lethal with mutations of genes encoding
basement membrane components and receptors due to pharyngeal or hypodermal defects. This indicates that TEN-1 could
act redundantly with integrin INA-1, dystroglycan DGN-1, and laminin EPI-1 in C. elegans development. Moreover, ten-1
deletion sensitizes worms to loss of nidogen nid-1 causing a pharynx unattached phenotype in ten-1;nid-1 double mutants.
We conclude that TEN-1 is important for basement membrane maintenance and/or adhesion in particular organs and
affects the function of somatic gonad precursor cells.
Teneurins are large transmembrane proteins that play im-
portant roles in cell signaling and cell adhesion (Tucker and
Chiquet-Ehrismann, 2006; Tucker et al., 2007). Teneurins are
phylogenetically conserved among metazoans and they
were described in several species, including ten-1 in Caeno-
rhabditis elegans (Drabikowski et al., 2005), ten-m/odz and
ten-a in Drosophila (Baumgartner et al., 1994; Levine et al.,
1994; Fascetti and Baumgartner, 2002; Rakovitsky et al.,
2007), zebrafish (Mieda et al., 1999), and in chicken (Minet et
al., 1999; Tucker et al., 2000; Tucker et al., 2001; Rubin et al.,
2002) and mouse (Oohashi et al., 1999; Ben-Zur et al., 2000;
Zhou et al., 2003). In vertebrates, the four teneurin paralogs
were named teneurin-1 to -4, ten-m1 to -m4, or odz-1 to -4.
The extracellular domain of all teneurins is composed of
eight tenascin-type EGF-like repeats, a region of conserved
cysteines, and YD repeats that are also found in a few
bacterial proteins (Minet and Chiquet-Ehrismann, 2000). The
intracellular domain contains proline-rich stretches and pu-
tative tyrosine phosphorylation sites but is less conserved
than the extracellular part and cannot be aligned in a linear
way between the phyla. Teneurins are thought to interact in
a homophilic manner (Oohashi et al., 1999; Rubin et al., 2002;
Bagutti et al., 2003; Leamey et al., 2008) and to date, no other
ligand has been identified.
The name "teneurins" refers to their high expression in
the developing and adult nervous system (Mieda et al.,
1999; Oohashi et al., 1999; Otaki and Firestein, 1999; Ben-
Zur et al., 2000; Tucker et al., 2000; Rubin et al., 2002; Zhou
et al., 2003). In the developing mouse cortex, all teneurins
are expressed in distinctive gradients and may be re-
quired for neocortical patterning (Li et al., 2006). Several
reports point out their role in the development of visual
pathways. Leamey et al. (2008) have found that teneurins
are up-regulated in visual versus somatosensory areas of
the neocortex. Moreover, expression of different teneurins
is largely nonoverlapping and can be found in intercon-
nected regions of the developing visual system (Rubin et
al., 1999, 2002; Kenzelmann et al., 2008; Leamey et al.,
2008). For instance, teneurin-1 staining is found in the
tectofugal pathway, and teneurin-2 is primarily expressed
in the thalamofugal pathway. In addition, teneurins were
shown to promote neurite outgrowth in vitro (Minet et al.,
1999; Rubin et al., 1999) and in vivo (Leamey et al., 2008),
suggesting an important function for teneurins in axon
guidance and target recognition. Recently, the first verte-
brate teneurin knockout was described (Leamey et al.,
2007). Teneurin-3 regulates eye-specific patterning in the
This article was published online ahead of print in MBC in Press
on July 16, 2008.
Address correspondence to: Ruth Chiquet-Ehrismann (Ruth.Chiquet@
Abbreviations used: BM, basement membrane; DIC, differential in-
terference contrast; L1, first larval stage; L2, second larval stage; L3,
third larval stage; L4, fourth larval stage; Pun, pharynx unattached;
SGP, somatic gonad precursor cells.
3898 © 2008 by The American Society for Cell Biology
Page 2
visual system, and the knockout mice show impaired
binocular vision.
Beside prominent expression in the nervous system, te-
neurins are also found in nonneuronal tissues. They are
expressed in alternating parasegments in the fly embryo, as
well as in cardiac cells, muscle attachment sites, and the
tracheal system in Drosophila (Baumgartner and Chiquet-
Ehrismann, 1993; Baumgartner et al., 1994). In the chicken
teneurins are found in limb buds, branchial arches, and
somites (Tucker et al., 2000, 2001), and in C. elegans ten-1 is
expressed in gonadal somatic cells, pharynx, and muscles
(Drabikowski et al., 2005). Teneurin expression in each of
these tissues is often associated with pattern formation and
cell migration.
The in vivo function of teneurins is mainly inferred from
studies of C. elegans and Drosophila mutants. Mutation of the
fly ten-m gene causes embryonic lethality due to the fusion
of adjacent denticle belts (Baumgartner et al., 1994; Levine et
al., 1994). Moreover, defects in the ventral nerve cord, car-
diac cells and eye patterning are found in late ten-m mutant
embryos (Levine et al., 1994; Kinel-Tahan et al., 2007). Similar
defects in cuticle and eye development have been observed
for the second Drosophila teneurin gene, ten-a (Rakovitsky et
al., 2007). In C. elegans, deletion in the ten-1 gene causes a
pleiotropic phenotype, including gonad disorganization,
nerve cord defasciculation, and defects in distal tip cell
migration and axonal pathfinding (Drabikowski et al., 2005).
The single teneurin ortholog in C. elegans, ten-1, is under
control of alternative promoters giving rise to two protein
variants. The isoforms differ only in their intracellular do-
mains. Their expression patterns are complex but mostly
nonoverlapping: TEN-1 long (TEN-1L) is found mainly in
the mesoderm, including pharynx, somatic gonad, and var-
ious muscles and neurons, and TEN-1 short (TEN-1S) is
predominantly expressed in some hypodermal cells and in a
subset of neurons (Drabikowski et al., 2005).
We report here the role of TEN-1 in gonadal basement
membrane maintenance, as well as in epidermal and pha-
ryngeal development. Mutation of the ten-1 gene leads to
gonad rupture and sterility. Germ cell leakage from the
gonads has also been reported for basement membrane mu-
tants, e.g., integrin ? ina-1, dystroglycan dgn-1, and laminin
?B epi-1 (Baum and Garriga, 1997; Huang et al., 2003; John-
son et al., 2006). Furthermore, the genetic interactions be-
tween ten-1, ina-1, dgn-1, epi-1, and nid-1 suggest that te-
neurin, integrin, and dystroglycan have related and partly
redundant functions in C. elegans development.
General Methods and C. elegans Strains
C. elegans strains were maintained at 20°C as described (Brenner, 1974). The
following strains were used in this study: wild-type N2, variety Bristol,
CH120: cle-1(cg120) I, CB444: unc-52(e444) II, VC518: ten-1(ok641) III; TM0651:
ten-1(tm651) III; NG39: ina-1(gm39) III; NG144: ina-1(gm144) III; CB189: unc-
32(e189) III; CX2914: nDf16/dpy-17(e164) unc-32(e189) III; CH119: nid-1(cg119)
V; CH121: dgn-1(cg121)/dpy-6(e14) unc-115(mn481) X. The tm651 deletion re-
moves nucleotides R13F6: 3661-4550 of the ten-1 coding sequence.
The following GFP marker strains were used: RU7: kdEx7 [ten-1a::gfp];
RU97: ten-1(ok641) kdEx45 [F36A3, III]; JK2049: qIs19 [lag-2::gfp]; SS0747: bnIs1
[pie-1::GFP::PGL-1] (gift of Susan Strome, University of California, Santa Cruz,
CA); IM253: urEx131 [lam-1::gfp] (gift of William Wadsworth, Robert Wood
Johnson Medical School, Piscataway, NJ), CH1878: dgn-2(ok209) dgn-3(tm1092)
dgn-1(cg121); cgEx308 [DGN-1::GFP] (gift of James Kramer, Northwestern
University Medical School, Chicago, IL).
Double mutant worms were maintained as [ten-1(ok641);ina-1(gm144);
kdEx45], [ten-1(ok641/?);nid-1(cg119)], [ten-1(ok641);dgn-1(cg121/?); kdEx45] or
[ten-1(ok641/?);dgn-1(cg121); cgEx308] strains and genotyped by PCR for the
phenotypic analysis.
Constructs and Plasmids
The translational Pten-1a::GFP::TEN-1L minigene reporter construct was gen-
erated by cloning SpeI-HindIII cDNA fragment and HindIII-XhoI genomic
fragment of TEN-1 long variant into p123T vector (Mo Bi Tec, Goettingen,
Germany). The following restriction sites were introduced into the primers:
SpeI and XhoI flanking the ten-1 coding sequence, SacII at the 5? end of the
ten-1a promoter, and ApaI downstream of the 3? UTR.
The long intracellular domain, transmembrane domain, and a short frag-
ment of the extracellular part were amplified using 5?-AACAGTCTAC-
CGAATCCCAACC-3? and 5?-ATAACTAGTATGTTCCAGCACAGGTAAA-
CTACCACG-3? primers and cDNA from mixed stage N2 worms as a tem-
plate. For the extracellular domain of ten-1 we used 5?-GCTGAAATAC-
CCACTCGCCAGC-3? and 5?-ATCTCGAGCTATTCAGATTTTCGGAACT-
TCC-3? primers and R06H12 cosmid as a template. The sequence encoding
green fluorescent protein (GFP) was amplified from pPD117.01 vector and
its NcoI site was mutated to CCTTGG. GFP was fused by PCR to the
N-terminus of the ten-1 cDNA fragment, which was cloned into SpeI-NcoI
sites of ten-1 minigene. Hemagglutinin (HA) tag was added at the C-
terminus of ten-1 coding sequence by PCR and cloned into HpaI-XhoI sites.
The Pten-1a::GFP::TEN-1L construct contained 4235 base pairs of the ten-1a
promoter and a 512-base pair sequence downstream of the stop codon. PCR
fragments were generated with Pfu Turbo DNA polymerase (Stratagene,
La Jolla, CA).
Transgenic Animals
Transgenic lines were generated as previously described (Mello et al., 1991).
The Pten-1a::GFP::TEN-1L plasmid was injected into ten-1(ok641) mutant
worms. Injections of GFP::TEN-1 minigene at low concentration (5 ng/?l)
resulted in a very weak GFP fluorescence, mainly in the nervous system.
Therefore, we injected the worms with high concentrations of the transgene
(40 ng/?l) and obtained several lines giving stronger GFP fluorescence. We
used pRF4 [rol-6] as a coinjection marker. This resulted in the line RU152:
kdEx121 [Pten-1a::GFP::TEN-1L] used in this study.
RNA Interference
RNA-mediated interference (RNAi) was performed as described (Kamath and
Ahringer, 2003). The K08C7.3 RNAi clone was obtained from the Ahringer
feeding library. Wild-type and ten-1(ok641) synchronized L4 hermaphrodites
were placed on RNAi plates and grown at 15°C for 72 h. Single adult worms
were placed on fresh RNAi plates and allowed to lay eggs for 24 h. These
plates were examined for 3 d to determine embryonic lethality and postem-
bryonic phenotypes.
Immunostaining of C. elegans Larvae
C. elegans larvae were prepared as previously described (Finney and Ruvkun,
1990). Fixed animals were blocked overnight at 4°C in PBS containing 0.1%
Triton X-100 (Triton) and 10% goat serum. Samples were incubated with an
antibody against collagen IV LET-2 (NW68, kind gift of James Kramer)
overnight at 4°C, washed in PBS containing Triton, and incubated with
fluorescein conjugated goat anti-rabbit secondary antibody overnight at room
temperature. Finally, fixed larvae were washed in PBS containing Triton and
Hoechst, followed by PBS alone.
Electron Microscopy
Worms were washed in M9 and anesthetized in 8% ethanol in M9 for 5 min.
They were placed in a fixative (2.5% glutaraldehyde, 1% paraformaldehyde in
0.1M sucrose, and 10 mM PBS, pH 7.4), cut open with a needle at both anterior
and posterior ends, and fixed for 2 h. Worms were embedded in 2% agarose,
cut into small blocks, and washed three times in PBS. Subsequently, pieces
were fixed with a second solution (1% osmium tetroxide, 1.5% potassium
ferrocyanide in PBS) for 2 h and washed three times in water. Worms were
stained with 1% uranyl acetate for 1 h. Samples were dehydrated in ethanol
(10 min in 50% ethanol, 10 min in 70% ethanol, 10 min in 90% ethanol, and 10
min in 100% ethanol) and acetone (10 min). Blocks with worms were embed-
ded in Epon resin (Fluka, Buchs, Switzerland): first in Epon-acetone (1:1) for
1–2 h and then in pure resin for 2–4 h. Samples polymerized for 24–48 h at
60°C and in 60-nm sections were prepared with Ultracut E. Sections were
stained in uranyl acetate for 60 min and then 2 min in Millonig's lead acetate
stain. Pictures were taken on Philips Morgagni 80 KV microscope (Eindhoven,
The Netherlands).
Phenotypic Analysis
Young adult hermaphrodites were placed on separate plates and allowed to
lay eggs for 24 h. The progeny were analyzed for embryonic and postembry-
onic phenotypes: lethality, larval arrest, sterility, and bursting at the vulva.
TEN-1 Function in Basement Membranes
Vol. 19, September 20083899
Page 3
Time Course of Germline Development and Basement
Membrane Breakdown
Synchronized, starved L1 larvae carrying the GFP::PGL-1 marker were placed
on bacteria plates. We scored the number of germ cells in 20 worms for each
genotype at 0, 8, 12, 16, and 20 h. For the study of basement membrane
integrity, we used synchronized worms carrying the LAM-1::GFP marker and
analyzed 50–62 worms for each developmental stage.
Animals were mounted on 2% agarose pads in a drop of M9 buffer containing
25 mM sodium azide. Differential interference contrast (DIC) and fluorescence
images were acquired with Z1 microscope (Zeiss, Jena, Germany) and Axio-
Cam Mrm camera (Zeiss) using 63?/1.4 NA Plan-Apochromat objective
(Zeiss) and AxioVision software.
Both ten-1(ok641) and ten-1(tm651) Are Functional Null
In our previous study we described the ten-1 mutation,
ok641, that carries an in-frame 2130-base pair deletion re-
moving four EGF-like repeats and a large part of the con-
served cysteines region (Drabikowski et al., 2005). We now
obtained another allele, tm651, lacking 890 base pairs and
introducing a frameshift into the ten-1 coding sequence (Fig-
ure 1). This deletion results in a loss of the transmembrane
domain and the entire extracellular part. Therefore, tm651 is
most likely a null allele. Because phenotypes of both ten-1
mutants show similar penetrance (Table 1), we assume that
ok641 represents a functional null allele as well.
To confirm this hypothesis, we created heterozygous
worms carrying nDf16 deficiency in trans to tm651 or ok641
and investigated whether the mutant phenotypes became
aggravated after complete removal of one copy of the ten-1
gene. The ok641/nDf16 and tm651/nDf16 worms displayed
a similar range of defects to ok641 and tm651 homozygous
animals, and the values observed were very close to those
calculated under the assumption of ten-1 mutants being null
alleles (Table 2).
These data and the fact that ok641 and tm651 deletions
affected protein regions that are common to both TEN-1
isoforms, suggested that there was no functional TEN-1
present in any of the ten-1 mutants.
Gonads of ten-1 Mutant Worms Burst Early in
Previous studies demonstrated that TEN-1 plays an impor-
tant role in gonad development and function (Drabikowski
et al., 2005). Homozygous ten-1(ok641) worms are viable, but
15–20% are sterile or burst-through-the-vulva due to germ
cell leakage in the middle of the gonad. Occasionally, go-
nads disintegrate completely and germ cells float in the
pseudocoelom. We could rescue gonadal and vulval defects
by expression of the kdEx121 transgene encoding the long
teneurin isoform under its own promoter (Table 1).
To determine the basis and the developmental stage of
gonad bursting, we performed a time course experiment of
germ cell proliferation in the early gonads of ten-1(ok641)
mutants. We used worms carrying a P-granule GFP marker
to distinguish between germ cells and somatic gonad pre-
cursor cells. Interestingly, we found that germ cells were
released from the gonads of ten-1 mutant already at the early
L3 stage (Figure 2B). At the same time point, there were no
germ cells present around the developing somatic gonad
primordium in the wild-type worms (Figure 2A). A sharp
DIC boundary surrounding the gonad was visible in the
Figure 1.
gene and location of tm651 and ok641 dele-
tions. Exons are depicted as boxes and introns
are shown as lines. Expression of ten-1 is reg-
ulated by alternative promoters: ten-1a and
ten-1b, resulting in two type II transmembrane
protein variants differing in the length of their
Genomic organization of ten-1
intracellular domain. Fragments of exons encoding different protein domains are labeled as follows: red, single transmembrane domain,
green, EGF-like repeats in two groups, yellow, region of conserved cysteines, and blue, stretch of YD repeats. Black horizontal lines show the
regions deleted in two ten-1 mutants: tm651 and ok641.
Table 1. Phenotypes of ten-1 deletion mutants
GenotypeEmbryonic lethality (%) Larval arrest (%)Sterile and/or vulva defects (%)Fertile adults (%)n
Wild type
ten-1(ok641), kdEx121
Table 2. Embryonic lethality and larval arrest phenotypes appearing in the progeny of nDf16/ten-1 unc-32 transheterozygotes
GenotypeEmbryonic lethality (%)Larval arrest (%)Adults: total (%)% Unc in adult wormsn
nDf16/ten-1(tm651) unc-32(e189)
nDf16/ten-1(ok641) unc-32(e189)
Expected value for nDf16/ten-1a
aThe calculated ratio of phenotypes expected if the ten-1 mutants are null mutants.
A. Trzebiatowska et al.
Molecular Biology of the Cell 3900
Page 4
wild type as well as a large part of ten-1(ok641) gonad
(Figure 2, A and B) but absent on the dorsal side of the
mutant gonad, where the germ cells leaked out into the
pseudocoelom. Gonad bursting was not the result of germ-
line overproliferation causing increased pressure on the go-
nadal basement membrane (BM), because we did not find
any difference in the number of germ cells between wild-
type and ten-1 mutants at this stage (Figure 2C).
Gonadal Basement Membrane Is Not Maintained in the
ten-1 Mutant
Bursting of the early gonads in the ten-1 mutant suggested
that mutant worms have defects in BM formation or main-
tenance. Therefore, we examined the organization of
the BMs in the ten-1(ok641) worms using a laminin-?
LAM-1::GFP marker and an anti-collagen IV antibody that
label most BMs in worms.
At hatching, wild-type and the majority of ten-1 mutant
gonad primordia were compact and completely surrounded
by laminin (Figure 3, A and B). At the L2 stage the laminin
layer surrounding the developing gonad of the mutants
appeared to get thinner at the dorsal side, but germ cells
did not lose contacts and gonads kept their tubular shape,
similarly to wild-type (Figure 3, C and D). As the gonadal
precursor cells divided, a discontinuity appeared in the
ten-1(ok641) gonadal BM that could be seen by a lack of
laminin as well as of collagen IV LET-2 (Figure 3, C and
E). In L3 larvae germ cells were released in the center of
the mutant gonad, where there was no laminin-GFP de-
tectable. Gonad disruption appeared always on the dorsal
side, whereas gonad arms were normally covered with
BM (Figure 3F). In wild-type animals the gonads re-
Figure 2.
1(ok641) mutant through the central break. Germ cell number and
localization wereevaluated
pie-1::GFP::PGL-1. (A) Wild-type L3 gonad. The somatic gonadal
primordium forms in the middle of the gonad, and germ cells fill the
two gonad arms (only one arm is shown). (B) Ruptured gonadal
primordium of a ten-1(ok641) L3 larva. Germ cells are released into
the body cavity and localize in the vicinity of the developing so-
matic gonad primordium. (C) Time course of germline development
in wild-type animals and ten-1(ok641) mutants. There is no germline
overproliferation in the early gonads of the ten-1(ok641) mutant.
Scale bar, 20 ?m.
Germ cells are released from the early gonad of ten-
using theP-granulemarker
Figure 3.
LAM-1::GFP marker (A–D and F–G) and the anti-LET-2 immunostaining (E). The ten-1(ok641) L1 gonad (A), wild-type L1 (B), and L2 (D)
gonads are uniformly covered by laminin. (C) In the ten-1(ok641) mutant, the gonadal basement membrane becomes thinner or fails to
assemble correctly (arrowhead) at the L2 stage. (E) Lack of gonadal BM on the dorsal side of ten-1(ok641) L2 gonad is visualized by collagen
IV immunostaining with anti-LET-2. (F) There is no laminin present in the center of the ten-1(ok641) L3 gonad. The basement membrane is
absent completely, and germ cells are released. (G) The wild-type L3 gonad is entirely covered by laminin. (H) Time-course analysis of
gonadal BM integrity in ten-1(ok641) worms carrying the LAM-1::GFP marker. Scale bar, 20 ?m.
The basement membrane breaks on the dorsal side of the ten-1(ok641) gonads. Basement membranes were visualized by the
TEN-1 Function in Basement Membranes
Vol. 19, September 20083901
Page 5
mained completely ensheathed by a BM (Figure 3G). A
time course of the appearance and the penetrance of BM
defects in young ten-1(ok641) larvae is summarized in
Figure 3H.
Furthermore, we compared the BM ultrastructure in wild-
type and ten-1 mutant worms using transmission electron
microscopy of thin sections of L3 larvae. Wild-type gonads
were completely ensheathed by BM, which appeared as a
thin mesh of extracellular material along the plasma mem-
branes (Figure 4, B and B1). In ten-1(ok641) worms, gonads
had a round shape and were entirely covered by BM in
sections localized distally from the break (Figure 4C and
C1). However, in the midbody region no BM was present
on the dorsal side of the broken gonad and germ cells
invaded the intestine (Figure 4, D and D1). In contrast, the
BMs on the ventral side of the burst gonad as well as the
BMs between the intestine and the hypodermis showed a
wild-type ultrastructure (Figure 4, D and D2). Moreover,
we did not find any whorls or clumps of extracellular ma-
terial that might suggest a general defect in BM organiza-
tion. Such a phenotype was described for some BM mutants
such as epi-1, lam-1, or dig-1 (Huang et al., 2003; Benard et al.,
2006; Kao et al., 2006).
In summary, the gonadal BM in the ten-1(ok641) hermaph-
rodites was properly assembled at hatching but was not
maintained later in development. The localized BM defi-
ciency could result from defects in BM assembly, stability, or
protein expression.
Gonadal Defects of ten-1 Mutants Are Similar to Those
Found in the Dystroglycan dgn-1, Integrin ina-1, and
Laminin epi-1 Mutants
Laminins are secreted proteins that play fundamental roles
in BM formation and function (Previtali et al., 2003; Miner
and Yurchenco, 2004). EPI-1 is one of two laminin ? chains
found in the C. elegans genome. Both C. elegans laminin
isoforms are broadly distributed among BMs, but the go-
nadal BM contains the EPI-1 isoform only (Huang et al.,
2003). Dystroglycan and integrins, two cell surface receptors
interacting with laminin, are required for BM assembly,
adhesion, and signal transduction (Bokel and Brown, 2002;
Higginson and Winder, 2005). In C. elegans, gonadal epithe-
lialization defects were reported for the dystroglycan dgn-
1(cg121) worms and laminin ? chain epi-1 mutants (Huang et
al., 2003; Johnson et al., 2006). Gonads of integrin ? chain
ina-1 mutants are oddly sized and show germ cell leakage,
but the cause of the defects remains unknown (Baum and
Garriga, 1997).
Gonads of dgn-1 mutants and epi-1(RNAi) worms were
variably misshapen (Figure 5, C and E), burst during devel-
opment, and led to worm sterility. Early gonads of ina-
1(gm39) worms hardly ever burst (Figure 5F) and rather
seemed to be swollen in the center. However, at the L4 stage
ina-1 mutant gonads were clearly ruptured, and the germ
cells clustered around the developing vulva (Figure 5G),
similarly to ten-1(ok641) gonads (Figure 5H). Gonads of
adult worms carrying the weaker ina-1 allele, gm144, had
enlarged arms but we did not observe any germ cell leakage
(unpublished data).
We analyzed the organization of the laminin network
surrounding the developing gonad in dgn-1 mutants using
the LAM-1::GFP marker. Although the DIC pictures of ten-1
and dgn-1 mutants appeared similar, dgn-1(cg121) hermaph-
rodite gonads did not have any localized breaks as did the
ten-1(ok641) gonads. In contrast, the dgn-1 mutant gonads
were generally disorganized, and LAM-1::GFP seemed to be
more diffuse throughout the gonadal surface in comparison
to ten-1 mutant gonads (Figure 5D). Nevertheless, gonadal
defects described for dgn-1(cg121), ina-1(gm39), and epi-
1(RNAi) worms resembled the defects that we observed in
the ten-1 mutants (Figure 5B), suggesting that TEN-1 could
be involved in gonadal BM maintenance, together with lami-
nin receptors INA-1 and DGN-1.
Figure 4.
worms. Schematic cross-section through the midbody of wild-type
worm (A). Transmission electron microscopy sections of a wild-type
L3 (B) and a ten-1(ok641) mutant (C and D). Tissues are labeled as
follows: blue, gonad; yellow, intestine; green, muscles; and gray/
unlabeled, hypodermis . Enlargements (B1–D2) are marked on the
cross-sections (B–D) with white rectangles. Morphology of wild-
type BMs at the boundaries between gonad and intestine (B1, ar-
rows). The gonadal and intestinal BM of the ten-1 mutant appears
wild-type in a section 2 ?m distant from the central break (C1,
arrows). In the midbody region, the mutant gonad breaks on its
dorsal side, and there is no BM present between germ cells and
intestine (D1, arrowhead). However, BMs between intestine and
hypodermis (D1, arrows) or ventral gonad and hypodermal ridge
(D2, arrows) have a normal ultrastructure. Scale bar, 5 ?m (A–D)
and 500 nm (B1–D2).
Basement membrane ultrastructure in ten-1 mutant
A. Trzebiatowska et al.
Molecular Biology of the Cell3902


text:
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Posted: 06/04/2008 - Some argue not needing a liquor license from the state gives tribal casinos unfair advantage.
Posted: 06/04/2008 - Canadian Auto Workers are not taking plant closure lying down.
Updated: 06/04/2008 - Eight months from the digital switch, people still have questions.
Posted: 06/04/2008 - Michigan wildlife officials may allow hunting of albino deer.
Updated: 06/04/2008 - Rabid bats are becoming more and more common throughout Mid-Michigan, and a vaccine shortage isn't helping.
Posted: 06/04/2008 - John McCain challenges Barack Obama to 10 town hall meetings
Posted: 06/04/2008 - Midwest hit by tornadoes and flooding
Posted: 06/04/2008 - United cuts staff, planes as it tries to save money.
Updated: 06/04/2008 - Obama told supporters in Minnesota he is the Democratic nominee, the first black man ever nominated by a major party.
Posted: 06/04/2008 - New jackpot is at least $36 million.
Updated: 06/04/2008 - Obama makes history as the first black presidential nominee.
Posted: 06/03/2008 - Looks like Lower Peninsula. No word if Yoopers are outraged.
Posted: 06/03/2008 - Helped push him to magic number for nomination.
Updated: 06/03/2008 - High gas is forcing schools to bulk up transportation budgets and make cuts elsewhere
Updated: 06/03/2008 - Truck sales are dipping because of the higher price of fuel and low gas mileage.
Posted: 06/03/2008 - About 150 of the those jobs will be cut from the headquarters in Ann Arbor.
Posted: 06/03/2008 - A man from Commerce Township and 2 men from New York are accused of planning to hire a hit man to kill a Guatemalan family.
Posted: 06/03/2008 - Pringles designer buried in one of his cans.
Posted: 06/03/2008 - Churchill Downs to honor Eight Belles
Posted: 06/03/2008 - The Sunday offering of the Calvary United Methodist Church in Jackson was stolen during a break-in.


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Interview: Ian Fetterman - Pumpkin Carver Extraordinaire
November 24th, 2014
Interviewed by: Horrornews.net
Interview: Mike T. Lyddon (Creepers)
November 20th, 2014
Interviewed by: Horrornews.net
Interview: Author Deana Demko
November 17th, 2014
Interviewed by: Janel Spiegel
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Book Review: Axes of Evil - Edited by Alex S. Johnson
November 22nd, 2014
Reviewed by: Dave Gammon
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Book Review: Prisoner 489 - Author Joe Lansdale
November 17th, 2014
Reviewed by: Anton Cancre
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Book Review: Equilibrium Overturned - Authors Anthony Rivera and Sharon Lawson
November 13th, 2014
Reviewed by: Dave Gammon
Category: Book Reviews
Film Review: The Prey (1984)
SYNOPSIS: A group of friends on a camping trip are terrorized by a horribly disfigured and psychotic survivor of a horrible forest fire that took place decades ago. As the members of the camping party start dying in a variety of ways at the hands of the hulking killer it becomes obvious that he isn't just looking for victims to butcher, but he is searching for a mate as well. REVIEW: I saw The Prey … Continue reading


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Off the Beaten Path Insider: Step Back Into America's Past in Historic DeLand
- Cultured
- Off The Beaten Path
Historic DeLand "just seems to embody everything that's good about Florida," says VISIT FLORIDA Insider Gary McKechnie. "It's a quiet town — there's just a sense of Americana here that you can't find in a lot of places." There are no chain stores here. Browse LPs at Groovy Records, or stroll through the Florida Museum for Women Artists. It's a perfect slice of pure Florida, and a wonderful place to spend an afternoon.


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Sea Pact announces grant recipients
By SeafoodSource staff
Published on 19 November, 2013
Sea Pact has announced that grant money will be distributed this month to four organizations doing work in seafood sustainability.
"Through the Sea Pact funding model we're able to support and encourage four innovative projects we feel will have a measurable impact on the future of our industry," said Logan Kock, Sea Pact advisory committee chair.
The four projects selected to receive funding are:
Panamanian Mahi Mahi FIP: funding will be used to build a fisheries monitoring program to understand biological aspects of mahi mahi and interactions of the fishery with other species. This project is being spearheaded by CeDePesca, an NGO based in Buenos Aires, Argentina
that focuses on sustainable fisheries improvement projects, research and training activities.
Pelagic Trawl Gear Improvement Project: funding will be used to help demonstrate that alternative trawl gear configurations with less habitat impact can land higher quality fish more quickly and with significantly lower fuel costs. Led by Fathom Consulting, a small fisheries consulting firm based in Monterey that works on fishery improvement projects that advance both fishery and conservation goals. Their clients include the Nature Conservancy, Environmental Defense Fund and NOAA.
Mexican Grouper FIP: funding will be used to develop a credible FIP that would be recognized by the Conservation Alliance for Sustainable Fisheries and would prepare the fishery to achieve full Marine Stewardship Council certification. Projected outcomes include stabilizing the Mexican grouper fishery, maintaining livelihoods for fishermen and fish workers, and potentially increasing benefits from a better market niche.
The NFI's Crab Council: funding will be used to supply equipment and training for assessing crab enhancement efforts in the Philippines blue swimming crab fishery, mainly through a tagging and tracking project.
A project under the new venture funds, Albion Fisheries, Fortune Fish & Gourmet, Ipswich Shellfish Group, Santa Monica Seafood, Seacore Seafood and Seattle Fish Co. are the founding members of Sea Pact. With New Venture Fund and receives sustainability counsel from non-profit organizations FishWise and Sustainable Fisheries Partnership (SFP).
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Norway salmon prices steady; Russia skirts trade ban21 11 2014
Higher demand in Russia and in other markets is contributing to strong Norwegian Atlantic salmon prices, while plentiful supply from Chile is keeping prices from that region soft. Norwegian and...
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Robert Zed named Dal's new Executive in Residence
Robert Zed will take on a new role at Dalhousie as the Executive in Residence at the University's School of Health Administration. Read more
Web-based tool aimed at helping improve care for those with atrial fibrillation
Dalhousie and Capital Health researchers are aiming to enhance the quality of life for people with atrial fibrillation through a new web-based tool. Read more
Canada's first endowed occupational medicine research chair established in NB
J.D. Irving, Limited has contributed $2 million to Dalhousie Medicine New Brunswick's Medical Research Campaign to establish the first endowed occupational medicine research chair in Canada. Read more
Dalhousie faculty instrumental in opening Rwanda's first medical simulation & skills centre
A new simulation and skills centre that allows a wide variety of health professionals and trainees to practice their patient care skills opened at the National University of Rwanda this summer. Dr. ... Read more
Dal receives $1.6M for new ocean sciences graduate-level training and research program
The Natural Sciences and Engineering Research Council of Canada has announced $1.6 million in funding for a new Collaborative Research and Training Experience (CREATE) program based at Dalhousie. The ... Read more
Considering the future of campus energy
Dalhousie is looking to become the first university in Atlantic Canada to generate its own electricity, as it considers a tri-generation system that would employ combustion turbine generators, fired... Read more
Arctic Ocean more vulnerable to human-induced changes than Antarctic Ocean
A team including Dalhousie Oceanography Professor Helmuth Thomas and recent Dalhousie Oceanography PhD graduate Elizabeth Shadwick found evidence suggesting that the Arctic Ocean is more vulnerable ... Read more
New funding for shark research in Nova Scotian waters
Dalhousie University's Ocean Tracking Network (OTN) has been awarded funding from Encana Corporation to study blue sharks off the coast of Nova Scotia, and to train students in the capture, ta... Read more
New Industrial Research Chair seeks ways to improve data transmission underwater
The Natural Sciences and Engineering Research Council of Canada (NSERC) and Ultra Electronics Maritimes Systems (UEMS) have partnered to award a $2.5 million Industrial Research Chair (IRC) to Dr. Chr... Read more
Faculty of Computer Science launches Canada's first big data research institute
Dalhousie University has launched the first academic research institute in Canada committed to expanding knowledge in the field of big data analytics. Read more


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A Revelation in Autumn
Barbour Pub., 2013 - Fiction - 124 pages
Book 5 of an exclusive 6-consecutive-month release Amish serial novel. In A Revelation in Autumn, part five of New York Times Bestselling author, Wanda E. Brunstetter's The Discovery--A Lancaster County Saga, Meredith Stoltzfus becomes closer to Jonah Miller and agrees to marry him in the spring of next year. About the same time, Luke, who is called "Eddie" by his nurse, is grateful for the nurse's grandparents who kindly take him in. Maybe living in a home-type atmosphere will help him regain his memory. Can he put the pieces of his past back together and return home in time—before he loses Meredith again?
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Review: A Revelation in Autumn (Discovery #5)User Review - Anne - Goodreads
Again, clearly my favorite Amish series ever. Read this book in one day. I love how each book has a cliffhanger. This one ends with a cliffhanger about Luke finding a memory. Read full review
Review: A Revelation in Autumn (Discovery #5)User Review - Linda Bourg - Goodreads
Enjoyed the fifth book in this series. At one point i was on the edge of my seat, and just wanted her to recognize Luke, but he was afar off so it didn't happen, that made me sad. But on to the next and final book of this series. I sure hope they find their way back to one another. Read full review


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The United Nations Conference on Environment and Development (UNCED), also known as The "Earth Summit", held in 1992, considered strategies reconciling the imperatives of environmental protection and worldwide development and adopted in Agenda 21 an international program of action for global sustainable development into 21st century.
On UNESCO initiative and with the close support of a group of Heads and Government, a World Solar Commission was established in 1995 to provide high-level leadership.
The Worlds Solar Summit held in 1997 launched The "Word Solar Programme 1996-2005 in which take part 104 States. This programme, conceived as a concrete follow-up of the recommendations of the Earth Summit.
The purpose of WSP is to sensitize governments, intergovernmental organizations, non-governmental organizations, financial institutions, academia and private institutions on the need to support the development and utilization of renewable energy for sustainable development. Objectives and actions to be taken through the high priority national, regional and global projects.
WSP recognize the significance of the role solar and other sources of renewable energy such as wind, geothermal, hydro, biomass and ocean. Solar energy can reduce environmental degradation caused by adverse human activities.
The strategy of renewables must be well-founded not only on technical and economical criterion, but on the society and energy link, social and cultural dimensions.
Among the many security issues related to energy, an issue of dominant concern is the growing dependence of the majority of developed and developing countries on imports from a few oil and gas producing countries.
It is clear that the rules of the market, without remedial measures, would hamper any resolute and rapid transition to renewable energy, despite its obvious societal, environmental and economic benefits.
WSP 1996-2005 is to become the main supporter of renewable energy development.
Decision-makers at the national level are increasingly aware of the fact that the wider-scale use of renewable energies can have a very important impact on two key issues confronting them: environmental protection and social development.
Several regions have planed that the share of renewables in the overall energy production should be in the range of 8 to 15% by the year 2005.
The EC predicts the contribution of renewable energy sources to gross inland renewable energy consumption to between 9,9% and 12,5% by 2010.
Lithuania has very few fossil sources of energy. The fossil sources are imported. On the other hand it has a sufficient solar, wind, hydro, biomass and geothermal resources. Lithuania has a scientific, technological and industrial potential for renewable energy development.
The development of renewable energy in Lithuania is guided by National program on increasing of an energy consumption effectiveness, the implementation of which lasts from 1992. The one of priority directions is utilization of biomes and other renewable sources of energy such as hydro, geothermal, particularly - wind and solar.
Lithuanian National Commission of UNESCO in common with Institute of Lithuanian Scientific Society under support by UNESCO carried out "The participation program 1998-1999" and has developed this "Lithuanian National Solar Program 2000-2005" and will take an effort to introduce it into World Solar Program 1996-2005.
Such national project of LNSP is developed. In fact this is Lithuanian program of a renewable energy obtaining nor only solar energy (photovoltaics, heat), but a biomes (plant biomes, biofuel, biooil), wind, hydro and geothermy as well. For this reason Lithuanian national solar program becomes Lithuanian renewable energy program.
The main goal of the Lithuanian National Solar Programmed as well, as Word Solar Program remains: to develop not only perspective researches, to create very effective technologies, to develop industry, to built demonstrations power systems, but, as it is very important, - to develop people education, to establish information set and communications, to implement sustainable renewable energy legislation.
Lithuanian National Solar Programme 2000-2005, as a part of the World Solar Program 1996-2005 (directed by UNESCO under United Nations), and as national program, correlated with EU White Paper: Energy for the Future: Renewable Sources of Energy, 1997, would become as a background for LITHUANIAN STATE PROGRAMME OF THE RENEWABLE SOURCES OF ENERGY .
[ Introduction ] [ Renewable energy in Lithuania ] [Research and technologies ] [ Demo power plants ]
[ Education and training ] [ Information and communication ] [ Legislation ]
[ Expert council ] [ Renewable energy server ] [ Lithuanian version ]


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The staff at Martinizing Dry Cleaning apply top-notch cleaning techniques to clothes and dispense their extensive knowledge to all customers. Get suits, dress shirts, and down jackets cleaned and dried, or have the cleaners sweep away stains, sweat, and teddy bear-size shoe prints from a comforter. Raincoats can find reprieve from damp days, and down coats are aired out and aptly prepped to face snowy ahead. The employees at Martinizing ensure garments return to customers soft, clean, and smelling fresh.
Walk through a field of wild flowers. Sit on the beach and listen to the flow of water falling ten feet into our 6000 gallon pond. Smell the roses climbing our custom gazebo. Lean on the split rail fence or sit along the stream and imagine where you will hang this personal piece of art Jeff is creating just for you. This ca
No two people are alike, so at Eclipse Studios, neither are two photo sessions. Senior portraits, for example, reflect the interests that make every teenager unique, whether they're nature lovers, sports fans, drama fanatics, or anything in between. Armed with decades of experience, Eclipse's photographer applies the skills and sensitivity required of virtually any situation. During boudoir sessions, they let women don their most elegant, but rarely flaunted ensembles and showcase their sensual sides amid flattering lighting and a comfortable environment. And family portraits celebrate the powerful connections between kin, whether in the formal setting of the studio or the candid atmosphere of a backyard get-together.
The hibernation experts at Mattress & Futon Shoppe help foster deep sleep with cushions from top brands such as Sealy, Stearns & Foster, and Corsicana. Well-versed in all forms of sleep habits, the store employees are happy to expound on the finer points of each mattress they keep in stock, which range from traditional innerspring beds to high-tech gel memory-foam mattresses. If your new mattress doesn't feel right or already has a family of monsters sleeping under it, Mattress & Futon Shoppe has a 100-day Don't Worry Sleep Happy comfort warranty, and you can return the unwanted item to one of 13 convenient locations.
Metro Detroit's full service supplier of stained , beveled glass and supply. Supplier of finished products and education for those interested in do-it-yourself projects.Expert instruction and thousands of options in glass and materials.
As one of Michigan's largest and oldest brokerage firms, Real Estate One shares nearly a century's worth of knowledge during its educational buffet of training courses. The firm's academy invites aspiring and established sales agents to sharpen their skills at classes held around the state, including a Michigan real estate licensing class for those hoping to sell property or additional locker space to gullible high school freshmen. Computer tutelage keeps students abreast of technological requisites, and continuing education programs help licensed agents meet state requirements. To ensure property apprentices graduate packed with knowledge, Real Estate One appoints experienced, respectful instructors to head each course.


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My Comments
Of course... THe OBVious lol He seems like down to earth friendly normal people heis SEXY but looks like just any normal person wasnt raised with a silver spoon n his mouth yea iDo like him mayne even love lol j/p
Replied To: do you really like trey (Forum topic)
On: Jul 08, 2010
Nope Only Long Term Thing n no im not tlkn abt his d*** lol it would be nice though lol.. *IM A LADY ALERT!*
Replied To: if you was still a virgin and trey songz asked you to loose your viginity with him would you or not? (Forum topic)
On: Jul 08, 2010
EFf th@ again *IM a LADY ALERT* lol But How far is far n ICould get to know him n let him move in with me or watever just not leave
Replied To: What would you do if Trey Songz called you and told you to pack all ur things n move with him? (Forum topic)
On: Jul 08, 2010
Thats pretty Scary
Replied To: My Trey Songz Quiz take it,,! (Forum topic)
On: Jul 08, 2010
no it wouldnt be a turnoff n say iD rather u pure n im one to lol itll be best because his pokey wasnt noWhere stinky n iM still wel yea u get it lol
Replied To: What would you do if Trey told you he was a virgin? (Forum topic)
On: Jul 08, 2010
Member name:
About Me:
iAm not a fan of Trey Songz.. Fans are just people. And iM not just people... iAm beyond a fan .. No iM not a Groupie. he's more of an Idol. THe SEXIEST idol ever. lol But No iAm not a Fan but in the same sentence iAm.. IDK wat to call it..
United States


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As part of our efforts to improve the way Council information is delivered to you we are now offering an email alert service to keep you updated on Council services that you are interested in.
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Welcome to the news room where you can keep up to date with all the latest news from Stockport Council.
Information on the latest scheduled roadworks taking place in Stockport.
All the political parties that make up the Council have signed a statement showing their support to safeguard young people at risk of child sexual exploitation.
'SoRT IT' is Stockport's approach to Neighbourhood Restorative Justice Panels (NRJP).
The Safer Stockport Partnership, which includes the Council and GMP, is offering £35,000 of funding for community activities and projects in Brinnington.
Young people are chasing the top jobs at Stockport Council!
Parents of 2, 3 and 4 year olds are being urged to protect them from flu this winter.
Stockport Council is supporting Alcohol Concern's 2015 Dry January campaign.
Over a 1,000 families from Stockport are now benefitting from free childcare funding for two year olds.
The result of the Bramhall South & Woodford Ward by-election held on Thursday 20th November 2014.
Since October over 500 residents have used the Council's new online form to report a faulty street light.
Residents have received their final reminder to make sure they are on the new electoral register before it is published for the first time on December 1st.
Stockport Council is encouraging residents to attend a Public Exhibition to look over proposals for a new business park in Cheadle Heath.
The Council has launched a new online Family Information Hub for services 'on offer' to children/young people with Special Educational Needs and/or Disability (SEND).
Stockport Council is supporting International Restorative Justice Week which runs from 16th to 23rd November.
Greater Manchester Councils have agreed an historic devolution settlement with the Government.
Council Leaders, housing companies and Japanese technologists will meet in Wigan to formally launch a 600-home smart heat project.
Stockport Council is supporting National HIV Testing Week which takes place between 22nd and 30th November.
Stockport Council is backing National Adoption Week in a bid to encourage more people to consider adopting.
Exciting events, festive markets, free parking and late night shopping are just some of the great things on offer in Stockport this Christmas.
Students and staff at Moat House (PRU) in Stockport are celebrating after they won the Gold Award in the category of Outstanding School Team of the Year 2014.
Free event highlights benefits of high speed broadband.
Stockport Council run Air Raid Shelters is commemorating the 100th anniversary of WWI and other conflicts with a Festival of Flowers.
88 people in Stockport are diagnosed with either bladder or kidney cancer every year and 46 people die from the diseases annually.
Parents of two, three and four year old children in Stockport are being urged to protect them from flu this winter.
Play a part in the future of the borough by giving your opinion on a range of proposals to shape future services
Stockport Council held a special event to recognise the excellent contribution made to the fostering service by sons and daughters of local foster carers.
Stockport Council and partners are supporting the North West campaign 'Let's Look Again at Alcohol' launched this week by the campaign group Drinkwise.
With the clocks going back this weekend residents are being reminded that any street lighting faults can now be reported quickly online.
Stockport Council is running a campaign to recruit more foster carers for older children and brothers and sisters.
Quitting smoking is a difficult thing to do at any age, but Bob, 62, from Cheadle, is showing it's never too late to quit.
Greater Manchester residents can find out how they can slash their fuel bills at the click of a mouse after a special web service was launched.
People across Stockport will be encouraged to take a more active role in civic life as part of Local Democracy Week.
Vehicles, which have exhaust emissions below 100g/km, are eligible for free parking in on- and off- street public parking places operated by the Council.
From October 16th Stockport Council employees will be visiting residents' homes who have not yet returned their Invitation to Register forms.
Stockport Council has announced today (Wednesday 15th October) that its £45 million Bridgefield development in Stockport town centre has been renamed "Redrock Stockport".
Residents across Stockport are being challenged to get online this October and try doing everyday things the digital way as part of the national Get Online Week.
More than 500 Stockport businesses and homes can now benefit from high-speed fibre broadband.
A pre-school nursery in Stockport held a special event to celebrate National Grandparents Day and helped promote free childcare for two year olds.
Shoppers and visitors to Stockport town centre will benefit from improved facilities in one of the borough's major car parks.
Three diesel-electric hybrid Metroshuttle buses have taken to the road in Stockport town centre.
Stockport Council is encouraging parents/carers to attend an information event to learn about the options available for young people when they leave school/ college.
Stockport Council has launched a host of new user-friendly improvements to its website, designed to make doing things simpler and quicker online.
Stockport residents have been signing up to take part in Stoptober throughout September and the mass 28-day quit smoking challenge has now officially started.
Stockport Council is marking Black History Month in October.
A campaign has started in Stockport to vaccinate people against winter flu.
Stockport resident Laura Sewell is encouraging Stockport's 40,000 smokers to sign up to Stoptober.
Stockport Council is taking immediate action today following an administrative error which meant some direct debits have been collected a day early.
Stockport Council, along with Stockport Homes and 15 other agencies across the borough have teamed up to hold a Wellbeing Week.
Stockport Council has submitted a planning application for new high quality sports and leisure facilities in Brinnington.
Following a restoration programme at the former Peaches Nightclub site on Wellington Street, now known as 'Heritage', over 50 high quality affordable homes are complete
Stockport Council has won a much sought after national award for its apprenticeship service.
As part of Stoptober Stockport Council is encouraging local residents to take advantage of the additional stop smoking local support available.
Stockport Council is backing the 2014 United Nations (UN) International Day of Peace on 21st September.
Stockport Council run Bramall Hall closes from Monday 29th September 2014 whilst repairs and improvements are carried out.
As of today, Stockport owners who have lost their dogs prior to the fire are being advised that they should contact Cheshire Dogs Home directly on 0844 504 12 12.
Stockport smokers encouraged to swap fags for gags as Stoptober 2014 launches
The Headteacher and Stockport Council today expressed their disappointment on the delay of the opening of the newly built Castle Hill High School.
Stockport Council has unveiled 'The Light Cinemas Ltd' as its operating partner for a brand new multiplex cinema at the Bridgefield development in Stockport Town Centre.
Visitors are invited to take one last look around Stockport Council run Bramall Hall before it closes for restoration and refurbishment at the end of September.
Stockport Council is asking residents, businesses and commuters for their views on proposals for the Stockport Town Centre Access Plan.
Stockport Council is inviting job seekers to attend Stockport's Jobs Fair on Tuesday 23rd September.
Stockport Council were involved in a joint operation with Greater Manchester Police to clamp down on non-compliant taxi drivers.
Plans are being made to boost the number of new homes for people to buy and to rent in Greater Manchester.
It is another successful year for pupils in Stockport following publication of the 2014 GCSE results for all the borough's secondary schools.
Stockport Council has created a number of graduate and apprenticeship employment opportunities.
Stockport Council and the Food Standards Agency (FSA) are urging people looking to round off the summer with a barbecue to do so safely.
Bright Beginnings Community Nursery in Adswood has helped launch Stockport's bright new look for promoting free childcare for two year olds.
Hundreds of residents have already re-registered to vote following a letter received by the Council informing them of the change to the electoral registration system.
Stockport Council is urging members of the public to come forward with any information about those responsible for graffiti on a War Memorial.
Stockport Council has a dedicated GCSE Exam Results Helpline to provide students with support and guidance.
Stockport residents living with dementia are set to benefit from improved care environments thanks to a funding boost.
A multi-million pound partnership which will make superfast fibre broadband available to thousands more businesses and homes in Greater Manchester.
The online application process for admissions to nursery, primary and secondary schools in September 2015 will open on Friday 15th August 2014.
Stockport Council will be consulting with local businesses and members of the public on plans for a brand new multiplex cinema and leisure complex in the town centre.
Stockport Council is supporting a major new summer campaign from Change4Life and Disney encouraging children to move more.
Stockport Council has been chosen to pilot a scheme which allows people approved to adopt to search the national Adoption Register for the first time.
Stockport Council in partnership with Lloyds TSB has launched a 'Local Lend a Hand' scheme to help first-time buyers and give the local housing market a boost.
Stockport Council is encouraging businesses across the borough to take part in the national 'Dementia – Be a Friend' campaign.
Stockport's outstanding and extraordinary citizens were honoured at the 'Proud of Stockport' awards in the Town Hall on Tuesday 4 February.


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The ability to ejaculate with force, accuracy and consistency
I showed high levels of spunkability last night.
by Andrew Topps October 24, 2004
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French bakery opens on St. Armands Circle
Date: October 12, 2009
by: Robin Hartill | Community Editor
Le Macaron pastry shop is now open at 382 St. Armands Circle. The French bakery currently serves 13 flavors of macarons, coffee, croissants and other pastries.
Rosalie Guillen and her daughter, Audrey Guillen Saba, who are originally from Provence, France, are the owners and bake all pastries in the shop. Hours are 10 a.m. to 9 p.m. Sunday through Thursday and 10 a.m. to 10 p.m. Friday and Saturday. For information, call 552-8872.
Contact Robin Hartill at email@example.com.
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- Nov
27 Thanksgiving Free Public Talk & Veggie Potluck
10:00 am - 3:00 pm
- Nov
29 Light Up Siesta Village and Holiday Parade
5:00 pm - 9:00 pm
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30 Advent Procession
6:00 pm
- Dec
3 Rachel's Challenge Anti-Bullying Event hosted by The Cal Ripken, Sr. Foundation
6:30 pm - 8:30 pm
Cops Corner: Sarasota
Enjoy this week's edition of Cops Corner.
World on a string
The exchange of goods and chatter are the usual soundtrack for the Saturday morning Downtown Farmers Market on Orange Avenue.
Read all about it
Bookstores across the state celebrated Florida Bookstore Day Saturday. Bookstore1 held an all-ages literary party and read-a-thon for customers and members of the community.


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U.S. Transportation Secretary Foxx Announces $840 Million Loan for the Grand Parkway Project in Greater Houston
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WASHINGTON – U.S. Transportation Secretary Anthony Foxx today announced the closing of an $840 million Transportation Infrastructure Finance and Innovation Act (TIFIA) loan for the Grand Parkway Project in greater Houston that will improve safety and add highway capacity in this fast-growing region of Texas.
"The Grand Parkway Project is just the latest example of how DOT is using innovative programs like TIFIA to help states and cities pursue their transportation priorities," said Secretary Foxx.
The completed Grand Parkway Project will be a four-lane, 55-mile toll road in northwest Harris County and southeast Montgomery County. The TIFIA loan will go toward the design and construction of specific sections of the project.
"Houston continues to grow and these improvements will help the region's mobility needs and further strengthen the region's economy," said FHWA Deputy Administrator Greg Nadeau.
The project is part of the broader Grand Parkway System – a proposed 184-mile highway that crosses seven counties and encircles the greater Houston metropolitan area from State Highway 146 in Galveston County to State Highway 146 in Baytown. The region served by the project has seen significant population growth over the last few decades – increasing about 6 percent per year. This growth has increased traffic demands and the need for additional capacity and highway improvements.
TIFIA provides federal credit assistance in the form of direct loans, loan guarantees, and standby lines of credit to finance surface transportation projects. It is designed to fill market gaps and leverage substantial non-federal investments. Each dollar of federal funding can provide up to $10 in TIFIA credit assistance and support up to $30 in transportation infrastructure investment. Since its launch, the TIFIA program has helped 40 projects turn almost $15.6 billion in DOT assistance into more than $58 billion in infrastructure investment across America. The transportation law, Moving Ahead for Progress in the 21st Century (MAP-21), transformed TIFIA into one of the largest transportation infrastructure loan programs in history, making up to $17 billion available in credit assistance for critical infrastructure projects.
News Source : U.S. Transportation Secretary Foxx Announces $840 Million Loan for the Grand Parkway Project in Greater Houston
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