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Flex project setup considerations review
You have seen some somewhat basic practices that, when followed, can foster a clean and understandable working environment for your Flex project. Consistency is a crucial element in understanding another developer's work. As applications mature, different developers can be called upon to modify and scale the app. During this time, a project's codebase shifts in an organic, lifelike way. This is due to the hands and minds of the multiple developers that contribute to the growth of the project. If a set of best practices is used from the beginning, there is a template to show the developers how to structure and format their work.
These practices can be applied to all of your Flex applications. Take a moment to review these practices:
Use workspaces to keep your Flex projects organized.
Use a common directory for your Flex workspace in team environments.
Follow common naming conventions for Flex project names.
Determine your target platform.
Use the default "bin-debug" directory.
Put your code in the "src" directory.
Use object-oriented programming practices for Flex projects.
Use a source code control solution.
Reference shared SWC libraries using the Flex Library path.
Use the libs folder to bundle SWC libraries with your Flex project.
Use UpperCamelCasing for MXML file names.
Use UpperCamelCasing for ActionScript Class file names.
Use UpperCamelCasing for ActionScript Interface file names, and begin the file name with a capitalized letter I.
Do not use spaces when naming files!
Do not use special characters when naming files.
Do not use acronyms or abbreviations.
Do not use periods in file names.
Use packages to organize and structure your projects codebase.
Use the following format for package names: com.seantheflexguy.stringUtils


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|first place
|I would love to have some points to make some contests
best critics ever gotten
~ Was this drawn on a moving ATV?
*want to be added give me a critic of how bad my art is*
Hello fellow deviants! Im Esprit-Arait but my real name is Haley. I am a overly hyper 16 year old girl with a passion for drawing, animation, and woodworking. I have never had someone tell me that i should give up on drawing. AND if someone does I will probably only do this in response... ignore them. but despite what i just said im actuly not a mean person unless you really start pushing my buttons. ok moveing past this subject... here are all the places you ca find me. (username)
~ www.Howrse.com (mudmask)
now people I know in real life that are on here
AKA my BFF
my Dad
my cousin
buddy from school
My altime biggest online insperation
Deviantart buddies
phobias: Apiphobia - fear of bees
Current Residence: Manitowoc, WI
Favourite genre of music: im ok with almost BUT heavy metal
Favourite photographer: Sooper-Deviant
Favourite style of art: Digital
Favourite cartoon character: sonic the hedgehog
Personal Quote: anyone can draw. it just takes some people longer than others to find THERE style.
my oc journal dolls
Thank you for the watch! ^_^

subset: commoncrawl


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Connect with Us!
11/30/2014CFP: 22nd Annual University of Texas at Arlington Student Conference in Linguistics & TESOL
12/1/2014CFP: DonQuixote in the American West: A Fourth-Centenary Celebration
12/5/2014CFP: Texas Foreign Language Education Conference
12/15/2014CFP: LinguaTechnology
12/17/2014CFP: I Congreso de Español como Segunda Lengua
Click on one of the following options for a description of the scholarship or travel stipend and application information.
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11/12/20142015 Conference Proposal Deadline Extended through 11/30/2014
11/10/20142014 Executive Council Election Results
11/2/2014AATSP remembers Lynn A. Sandstedt


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Under 15s
Lancs U15s Defeat Notts in their final game
This was the last game for the Under 15 county team this season as even if they won this game they could not progress further in Royal London U15 County Cup. ... Full story
5 Lancastrians Represent North of England
5 Lancashire Under 15s players represented the ECB North of England U15 in the recent ESCA Bunbury Festival at Oakham School from 21st -25th July 2014. ... Full story
Lancs U15s Defeated by Yorkshire
Lancashire Under 15s played Yorkshire Under 15s on Sunday 27 July at Urmston CC. ... Full story
Lancs U15s Fall Short Against Cheshire
Match report from Lancashire Under 15s v Cheshire Under 15s played on Wednesday 16 July at Neston CC. ... Full story
Lancs U15s Defeated by Derbyshire
Lancashire Under 15s lost to Derbyshire on Sunday 6th July away at Clifton CC. ... Full story
Lancs U15s Overcome Notts
Lancashire Under 15s battled well to overcome Nottinghamshire on Sunday 29 June. ... Full story
Lancs U15s Defeat Cheshire
Lancashire U15s 204 for 4 off 43.5 overs defeated Cheshire 200 for 7 off 50 overs at Urmston CC on Tuesday 17 June. ... Full story
Lancs U15s Triumph Over Yorkshire
Lancashire U15s played their second game in the ECB Royal London competition on Sunday June 8th. ... Full story
Match Report - Lancashire U15 vs Derbyshire U15
Sunday 1st June 2014. ... Full story
Lancashire Boys Under 15s 2014
U15s county details will be made available here. ... Full story
ECB Clubmark Accredited Clubs
The Lancashire Cricket Board, working in partnership with Greater Manchester Sports Partnership, Merseyside Sports Partnership and Lancashire Sports Partnership, would like to congratulate the following clubs on achieving the ECB Clubmark. ... Full story


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They Might Be Giants
They Might Be Giants has been perfecting its bite-size nerd rock over the past 30 years, carving out along the way as distinct an identity as any band working today. An album titled Nanobots (released in March), including 25 songs in 45 minutes, with titles such as "Circular Karate Chop" and "Insect Hospital"? That could only be the handiwork of the two Johns — Flansburgh and Linnell — who were geeks in Brooklyn wayyy before it was fashionable.
Thursday, June 6, at Crossroads KC at Grinders (417 East 18th Street, 785-749-3434)
Psychedelic Furs
English punks in the 1970s, new wave icons in the 1980s, splitsville in the 1990s, spotty reunions in the 2000s. What has this new decade brought the Psychedelic Furs so far? A tour paying tribute to the group's classic 1981 album, Talk Talk Talk, in 2011, and, this summer, another victory lap. Expect all the classics; there's been no new Furs material on record in 20 years.
Sunday, June 9, at the Midland (1228 Main, 816-283-9921)
Glaswegian trio Chvrches drives in the same lane as acts like Purity Ring and the Knife — woman-fronted electro-pop groups — but isn't quite so icy. Its 1980s-tinged synth hooks are approachable, if still a little menacing, and all signs point to this being a breakout year for the band: a buzzy SXSW, a tour with Depeche Mode and a debut due in the fall.
Friday, June 7, at the Granada (1020 Massachusetts, Lawrence, 785-842-1390)
Waiting for Signal
Mayday, the new album from locals Waiting for Signal, is the most recent addition to the catalog of albums practicing a genre that's sometimes referred to around here as the Kansas City Sound. It's dark, sometimes epic rock, with varying degrees of angsty emo and grunge tones. On Mayday, you can hear echoes of 1990s local legend Shiner, plus some of the muscle of acts like Muse and Audioslave. Sample it at this release show, at which Sons of Great Dane, Heroes and Villains, and Roman Ships are set to assist in the celebration.
Friday, June 7, at the Riot Room (4048 Broadway, 816-442-8179)
Crossroads Summer Block Party
Despite miserable heat, the first Crossroads Summer Block Party, last July, was a success. It returns this year to 19th Street (between Wyandotte and Baltimore) in the traditionally cooler month of June. Local label Golden Sound Records has again organized the party, and the lineup includes members of its roster, including Fullbloods, Hidden Pictures, and Millions of Boys. Also performing: Cowboy Indian Bear, Akkilles, Shy Boys, Oils and Opossum Trot. It's always nice when a show offers exposure to a lot of local talent in one fell swoop, and this is one of those shows. Bonus: It's free. The festivities get under way at 6 p.m., and the afterparty (at Snow & Co.) starts at midnight. See crossroadsblockparty.com for the schedule.
Friday, June 7, at 19th Street and Wyandotte
Lord Huron
As Lord Huron, Michiganer-turned-Angeleno Ben Schneider writes pretty, pastoral folk-rock. Its commonalities with the music of Fleet Foxes are well-noted, but on his most recent, Lonesome Dreams, I hear just as much of the, um, dreamy qualities I associate with Pacific Northwest group, the Helio Sequence. As a sound, it's nothing terribly novel given today's folk-drenched indie climate, but this guy's executing it better than a lot of the others.
Monday, June 10, at the Riot Room (4048 Broadway, 816-442-8179)


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Associated Press
D-M rescue group returns home after life-saving mission at sea
Two severely burned Chinese fishermen rescued by a group of Davis-Monthan airmen after their boat sank off the coast of Mexico could only say two words in English to the men who came to their aid: "Thank you."
Yuma motorcyclist dies after being hit by car
YUMA, Ariz. — Authorities say a 65-year-old man has died in Yuma after the motorcycle he was riding was rear-ended by a car.
Northwest Fire to decide on future of hotshot crew
Tucson's only hotshot crew believes it is about to be disbanded.
The power of dust storms
A blast from the past, literally. This shot was taken on April 15, 1935 in Boise City, Oklahoma. At that point, the high plains area was better known as the Dust Bowl or Dirty Thirties. Severe drought coupled with proper farming methods for dry land allowed wind erosion to create the giant s…
Heart of 'bionic man' came from Tucson
A "bionic man" showcased in a Smithsonian Channel documentary and exhibit was designed as an artificial human, though it lacks several working organs.
Trampoline park allows jumpers to 'Get Air'
Get Air Tucson, a trampoline park with 11,500 square feet of indoor trampolines, has opened its doors at 330 S. Toole Ave.
Cops: Fla. man kills wife, posts photo of body on Facebook
SOUTH MIAMI, Fla. — The South Florida man who authorities say killed his wife then apparently posted a photo of his 26-year-old wife's dead body on Facebook possessed a concealed weapon permit and portrayed himself as a neighborhood watch volunteer at his townhouse complex, fellow residents …
Night Stalker: Serial killer Richard Ramirez dies
LOS ANGELES — Richard Ramirez, the demonic serial killer known as the Night Stalker who left satanic signs at murder scenes and mutilated victims' bodies during a reign of terror in the 1980s, died early Friday in a hospital, a prison official said.
If you're going to defend the 1st Amendment, defend all of it
"Congress shall make no law respecting an establishment of religion, or prohibiting the free exercise thereof; or abridging the freedom of speech, or of the press; or the right of the people peaceably to assemble, and to petition the Government for a redress of grievances."
Gauen: Shield laws bring rules to the news leaking game
Search of AP phone records triggers fresh talk of reporter protections.
AP leak case isn't clear instance of gov't overreach
No justification for subpoena of AP records
The following editorial appeared Sunday in the Washington Post:
Media, tea party coalesce around common enemy
Breaking news: Conservative organizations suddenly have found common cause with one of their favorite objects of contempt - the benighted Mainstream Media.
Federal officials: No suspect in custody in Boston bombings
BOSTON — Police and reporters converged on the federal courthouse in Boston on Wednesday amid conflicting reports of whether a suspect was in custody in the marathon bombings that killed three people and wounded more than 170.
News media use array of questionable expressions
The fact that The Associated Press news agency decided to ban the term "illegal immigrant" last week is a big victory for fairness in journalism, but there are other terms used daily in the media that should be revised as well.
Roster of Missouri's sexual predators grows, and so does the bill
Projection expects number of people detained as potential sex predators to grow to 234 this year.
Guest opinion: Tucson Grammy nominee takes on Academy
2012 nominee for Best Americana Album says Academy of Recording Arts and Sciences changed the rules after her.
Stan Musial poses with wife Lil and daughter Janet
Stan Musial poses in the Polo Grounds with with wife Lil and daughter Janet after hitting four consecutive home runs in a doubleheader against the New York Mets in September of 1962. Associated Press Photo
Assaults on innocent life
Much has been and will be written following the horrific tragedy in Sandy Hook, Conn. Referring to that massacre, Ted Anthony of the Associated Press wrote that there will be "scores of grandparents who will look across generations and see less that they would have ... hundreds of accomplish…
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The present invention relates to the identification of a human gene family expressed in metabolically relevant tissues. The genes encode a group polypeptides referred to as "Protein Cluster V" which are predicted to be useful in the diagnosis of metabolic diseases, such as obesity and diabetes, as well as in the identification of agents useful in the treatment of the said diseases.
1. An isolated nucleic acid molecule selected from:
(a) nucleic acid molecules comprising a nucleotide sequence as shown in SEQ ID NO: 3, 5, 7, 9, 11, 13, 15, 17, or 19;
(b) nucleic acid molecules comprising a nucleotide sequence capable of hybridizing, under stringent hybridization conditions, to a nucleotide sequence complementary to the polypeptide coding region of a nucleic acid molecule as defined in (a); and
(c) nucleic acid molecules comprising a nucleic acid sequence which is degenerate as a result of the genetic code to a nucleotide sequence as defined in (a) or (b).
2. An isolated polypeptide encoded by the nucleic acid molecule according to
3. The isolated polypeptide according to
4. A vector harboring the nucleic acid molecule according to
5. A replicable expression vector which carries and is capable of mediating the expression of a nucleotide sequence according to
6. A cultured host cell harboring a vector according to
7. A process for production of a polypeptide, comprising culturing a host cell according to
8. A method for identifying an agent capable of modulating a nucleic acid molecule according to
(i) providing a cell comprising the said nucleic acid molecule;
(ii) contacting said cell with a candidate agent; and
(iii) monitoring said cell for an effect that is not present in the absence of said candidate agent.
[0001] The present invention relates to the identification of a human gene family expressed in metabolically relevant tissues. The genes encode a group polypeptides referred to as "Protein Cluster V" which are predicted to be useful in the diagnosis of metabolic diseases, such as obesity and diabetes, as well as in the identification of agents useful in the treatment of the said diseases.
[0002] Metabolic diseases are defined as any of the diseases or disorders that disrupt normal metabolism. They may arise from nutritional deficiencies; in connection with diseases of the endocrine system, the liver, or the kidneys; or as a result of genetic defects. Metabolic diseases are conditions caused by an abnormality in one or more of the chemical reactions essential to producing energy, to regenerating cellular constituents, or to eliminating unneeded products arising from these processes. Depending on which metabolic pathway is involved, a single defective chemical reaction may produce consequences that are narrow, involving a single body function, or broad, affecting many organs and systems.
[0003] One of the major hormones that influence metabolism is insulin, which is synthesized in the beta cells of the islets of Langerhans of the pancreas. Insulin primarily regulates the direction of metabolism, shifting many processes toward the storage of substrates and away from their degradation. Insulin acts to increase the transport of glucose and amino acids as well as key minerals such as potassium, magnesium, and phosphate from the blood into cells. It also regulates a variety of enzymatic reactions within the cells, all of which have a common overall direction, namely the synthesis of large molecules from small units. A deficiency in the action of insulin (diabetes mellitus) causes severe impairment in (i) the storage of glucose in the form of glycogen and the oxidation of glucose for energy; (ii) the synthesis and storage of fat from fatty acids and their precursors and the completion of fatty-acid oxidation; and (iii) the synthesis of proteins from amino acids.
[0004] There are two varieties of diabetes. Type I is insulin-dependent diabetes mellitus (IDDM), for which insulin injection is required; it was formerly referred to as juvenile onset diabetes. In this type, insulin is not secreted by the pancreas and hence must be taken by injection. Type II, non-insulin-dependent diabetes mellitus (NIDDM) may be controlled by dietary restriction. It derives from insufficient pancreatic insulin secretion and tissue resistance to secreted insulin, which is complicated by subtle changes in the secretion of insulin by the beta cells. Despite their former classifications as juvenile or adult, either type can occur at any age; NIDDM, however, is the most common type, accounting for 90 percent of all diabetes. While the exact causes of diabetes remain obscure, it is evident that NIDDM is linked to heredity and obesity. There is clearly a genetic predisposition to NIDDM diabetes in those who become overweight or obese.
[0005] Obesity is usually defined in terms of the body mass index (BMI), i.e. weight (in kilograms) divided by the square of the height (in meters). Weight is regulated with great precision. Regulation of body weight is believed to occur not only in persons of normal weight but also among many obese persons, in whom obesity is attributed to an elevation in the set point around which weight is regulated. The determinants of obesity can be divided into genetic, environmental, and regulatory.
[0006] Recent discoveries have helped explain how genes may determine obesity and how they may influence the regulation of body weight. For example, mutations in the ob gene have led to massive obesity in mice. Cloning the ob gene led to the identification of leptin, a protein coded by this gene; leptin is produced in adipose tissue cells and acts to control body fat. The existence of leptin supports the idea that body weight is regulated, because leptin serves as a signal between adipose tissue and the areas of the brain that control energy metabolism, which influences body weight.
[0007] Metabolic diseases like diabetes and obesity are clinically and genetically heterogeneous disorders. Recent advances in molecular genetics have led to the recognition of genes involved in IDDM and in some subtypes of NIDDM, including maturity-onset diabetes of the young (MODY) (Velho & Froguel (1997) Diabetes Metab. 23 Suppl 2:34-37). However, several IDDM susceptibility genes have not yet been identified, and very little is known about genes contributing to common forms of NIDDM. Studies of candidate genes and of genes mapped in animal models of IDDM or NIDDM, as well as whole genome scanning of diabetic families from different populations, should allow the identification of most diabetes susceptibility genes and of the molecular targets for new potential drugs. The identification of genes involved in metabolic disorders will thus contribute to the development of novel predictive and therapeutic approaches.
[0008] The β3-adrenergic receptor (AR) represents one of a number of potential anti-obesity drugs targets for which selective agonists have been developed. In rodents, β3-AR mRNA is abundant in white adipose tissue (WAT) and brown adipose tissue (BAT). It has been demonstrated that mice lacking endogenous β3-adrenoreceptors have a slight increase in body fat, but otherwise appear normal (Susulic V. S., et al. (1995) J. Biol. Chem. 270(49). 29483-29492). These-mice are completely resistant to the specific β3-agonist CL-316,243, which has been shown to increase lipolysis, energy expenditure and affect insulin and leptin levels. When the β3-AR was ectopically expressed in white and brown adipose tissue or brown adipose tissue only, it was recently demonstrated that the anorectic and insulin secretagogue effects appeared to be mediated by white adipose tissue (Grujic D, et al. (1997) J Biol Chem. 272(28): 17686-93). How these effects are mediated by β3-AR agonists remains poorly understood.
[0009] Lardizabal, K. D. et al. (J. Biol. Chem. 276: 38862-38869) and Cases, S. et al. (J. Biol. Chem. 276: 38870-38876; both papers published Jul. 31, 2001) disclose a new gene family, including members in fungi, plants and animals, which encode proteins corresponding to the "Cluster V" proteins according to the present invention. The proteins were shown to have acyl CoA:diacylglycerol acyltransferase (DGAT; EC 22.214.171.124) function. The gene family is unrelated to the previously identified DGAT(1) family and was designated DGAT2. DGAT2 was shown to have high expression levels in liver and white adipose tissue, suggesting that it may play a significant role in mammalian triglyceride metabolism.
[0010] According to the present invention, a family of genes and encoded homologous proteins (hereinafter referred to as "Protein Cluster V") has been identified. Consequently, the present invention provides an isolated nucleic acid molecule selected from:
[0011] (a) nucleic acid molecules comprising a nucleotide sequence as shown in SEQ ID NO: 3, 5, 7, 9, 11, 13, 15, 17, or 19.
[0012] (b) nucleic acid molecules comprising a nucleotide sequence capable of hybridizing, under stringent hybridization conditions, to a nucleotide sequence complementary to the polypeptide coding region of a nucleic acid molecule as defined in (a); and
[0013] (c) nucleic acid molecules comprising a nucleic acid sequence which is degenerate as a result of the genetic code to a nucleotide sequence as defined in (a) or (b).
[0014] The nucleic acid molecules according to the present invention includes cDNA, chemically synthesized DNA, DNA isolated by PCR, genomic DNA, and combinations thereof. RNA transcribed from DNA is also encompassed by the present invention.
[0015] The term "stringent hybridization conditions" is known in the art from standard protocols (e.g. Ausubel et al., supra) and could be understood as e.g. hybridization to filter-bound DNA in 0.5 M NaHPO4, 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at +65° C., and washing in 0.1×SSC/0.1% SDS at +68° C.
[0016] In a preferred form of the invention, the said nucleic acid molecule has a nucleotide sequence identical with SEQ ID NO: 3, 5, 7, 9, 11, 13, 15, 17, or 19 of the Sequence Listing. However, the nucleic acid molecule according to the invention is not to be limited strictly to the sequence shown as SEQ ID NO: 3, 5, 7, 9, 11, 13, 15, 17, or 19. Rather the invention encompasses nucleic acid molecules carrying modifications like substitutions, small deletions, insertions or inversions, which nevertheless encode proteins having substantially the features of the Protein Cluster V polypeptide according to the invention. Included in the invention are consequently nucleic acid molecules, the nucleotide sequence of which is at least 90% homologous, preferably at least 95% homologous, with the nucleotide sequence shown as SEQ ID NO: 3, 5, 7, 9, 11, 13, 15, 17, or 19 in the Sequence Listing.
[0017] Included in the invention is also a nucleic acid molecule which nucleotide sequence is degenerate, because of the genetic code, to the nucleotide sequence shown as SEQ ID NO: 3, 5, 7, 9, 11, 13, 15, 17, or 19. A sequential grouping of three nucleotides, a "codon", codes for one amino acid. Since there are 64 possible codons, but only 20 natural amino acids, most amino acids are coded for by more than one codon. This natural "degeneracy", or "redundancy", of the genetic code is well known in the art. It will thus be appreciated that the nucleotide sequence shown in the Sequence Listing is only an example within a large but definite group of sequences which will encode the Protein Cluster V polypeptide.
[0018] The nucleic acid molecules according to the invention have numerous applications in techniques known to those skilled in the art of molecular biology. These techniques include their use as hybridization probes, for chromosome and gene mapping, in PCR technologies, in the production of sense or antisense nucleic acids, in screening for new therapeutic molecules, etc.
[0019] More specifically, the sequence information provided by the invention makes possible large-scale expression of the encoded polypeptides by techniques well known in the art. Nucleic acid molecules of the invention also permit identification and isolation of nucleic acid molecules encoding related polypeptides, such as human allelic variants and species homologues, by well-known techniques including Southern and/or Northern hybridization, and PCR. Knowledge of the sequence of a human DNA also makes possible, through use of Southern hybridization or PCR, the identification of genomic DNA sequences encoding the proteins in Cluster V, expression control regulatory sequences such as promoters, operators, enhancers, repressors, and the like. Nucleic acid molecules of the invention are also useful in hybridization assays to detect the capacity of cells to express the proteins in Cluster V. Nucleic acid molecules of the invention may also provide a basis for diagnostic methods useful for identifying a genetic alteration(s) in a locus that underlies a disease state or states, which information is useful both for diagnosis and for selection of therapeutic strategies.
[0020] In a further aspect, the invention provides an isolated polypeptide encoded by the nucleic acid molecule as defined above. In a preferred form, the said polypeptide has an amino acid sequence according to SEQ ID NO: 4, 6, 8, 10, 12, 14, 16, 18 or 20 of the Sequence Listing. However, the polypeptide according to the invention is not to be limited strictly to a polypeptide with an amino acid sequence identical with SEQ ID NO: 4, 6, 8, 10, 12, 14, 16, 18 or 20 in the Sequence Listing. Rather the invention encompasses polypeptides carrying modifications like substitutions, small deletions, insertions or inversions, which polypeptides nevertheless have substantially the features of the Protein Cluster V polypeptide. Included in the invention are consequently polypeptides, the amino acid sequence of which is at least 90% homologous, preferably at least 95% homologous, with the amino acid sequence shown as SEQ ID NO: 4, 6, 8, 10, 12, 14, 16, 18 or 20 in the Sequence Listing.
[0021] In a further aspect, the invention provides a vector harboring the nucleic acid molecule as defined above. The said vector can e.g. be a replicable expression vector, which carries and is capable of mediating the expression of a DNA molecule according to the invention. In the present context the term "replicable" means that the vector is able to replicate in a given type of host cell into which is has been introduced. Examples of vectors are viruses such as bacteriophages, cosmids, plasmids and other recombination vectors. Nucleic acid molecules are inserted into vector genomes by methods well known in the art.
[0022] Included in the invention is also a cultured host cell harboring a vector according to the invention. Such a host cell can be a prokaryotic cell, a unicellular eukaryotic cell or a cell derived from a multicellular organism. The host cell can thus e.g. be a bacterial cell such as an E. coli cell; a cell from yeast such as Saccharomyces cervisiae or Pichia pastoris, or a mammalian cell. The methods employed to effect introduction of the vector into the host cell are standard methods well known to a person familiar with recombinant DNA methods.
[0023] In yet another aspect, the invention provides a process for production of a polypeptide, comprising culturing a host cell, according to the invention, under conditions whereby said polypeptide is produced, and recovering said polypeptide. The medium used to grow the cells may be any conventional medium suitable for the purpose. A suitable vector may be any of the vectors described above, and an appropriate host cell may be any of the cell types listed above. The methods employed to construct the vector and effect introduction thereof into the host cell may be any methods known for such purposes within the field of recombinant DNA. The recombinant polypeptide expressed by the cells may be secreted, i.e. exported through the cell membrane, dependent on the type of cell and the composition of the vector.
[0024] In a further aspect, the invention provides a method for identifying an agent capable of modulating a nucleic acid molecule according to the invention, comprising
[0025] (i) providing a cell comprising the said nucleic acid molecule;
[0026] (ii) contacting said cell with a candidate agent; and
[0027] (iii) monitoring said cell for an effect that is not present in the absence of said candidate agent.
[0028] For screening purposes, appropriate host cells can be transformed with a vector having a reporter gene under the control of the nucleic acid molecule according to this invention. The expression of the reporter gene can be measured in the presence or absence of an agent with known activity (i.e. a standard agent) or putative activity (i.e. a "test agent" or "candidate agent"). A change in the level of expression of the reporter gene in the presence of the test agent is compared with that effected by the standard agent. In this way, active agents are identified and their relative potency in this assay determined.
[0029] A transfection assay can be a particularly useful screening assay for identifying an effective agent. In a transfection assay, a nucleic acid containing a gene such as a reporter gene that is operably linked to a nucleic acid molecule according to the invention, is transfected into the desired cell type. A test level of reporter gene expression is assayed in the presence of a candidate agent and compared to a control level of expression. An effective agent is identified as an agent that results in a test level of expression that is different than a control level of reporter gene expression, which is the level of expression determined in the absence of the agent. Methods for transfecting cells and a variety of convenient reporter genes are well known in the art (see, for example, Goeddel (ed.), Methods Enzymol., Vol. 185, San Diego: Academic Press, Inc. (1990); see also Sambrook, supra).
[0030] Throughout this description the terms "standard protocols" and "standard procedures", when used in the context of molecular biology techniques, are to be understood as protocols and procedures found in an ordinary laboratory manual such as: Current Protocols in Molecular Biology, editors F. Ausubel et al., John Wiley and Sons, Inc. 1994, or Sambrook, J., Fritsch, E. E and Maniatis, T., Molecular Cloning: A laboratory manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 1989.
[0031] A family of homologous proteins (hereinafter referred to as "Protein Cluster V") was identified by an "all-versus-all" BLAST procedure using all Caenorhabditis elegans proteins in the Wormpep20 database release (http://www.sanger.ac.uk/Projects/C_elegans/wormpep/index.shtml). The Wormpep database contains the predicted proteins from the C. elegans genome sequencing project, carried out jointly by the Sanger Centre in Cambridge, UK and the Genome Sequencing Center in St. Louis, USA. A number of 18,940 proteins were retrieved from Wormpep20. The proteins were used in a Smith-Waterman clustering procedure to group together proteins of similarity (Smith T. F. & Waterman M. S. (1981) Identification of common molecular subsequences. J. Mol. Biol. 147(1): 195-197; Pearson W R. (1991) Searching protein sequence libraries: comparison of the sensitivity and selectivity of the Smith-Waterman and FASTA algorithms. Genomics 11: 635-650; Olsen et al. (1999) Optimizing Smith-Waterman alignments. Pac Symp Biocomput. 302-313). Completely annotated proteins were filtered out, whereby 10,130 proteins of unknown function could be grouped into 1,800 clusters.
[0032] The obtained sequence clusters were compared to the Drosophila melanogaster proteins contained in the database Flybase (Berkeley Drosophila Genome Project; http://www.fruitfly.org), and annotated clusters were removed. Non-annotated protein clusters, conserved in both C. elegans and D. melanogaster, were saved to a worm/fly data set, which was used in a BLAST procedure (http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/information3.html) against the Celera Human Genome Database (http://www.celera.com). Overlapping fragments were assembled to, as close as possible, full-length proteins using the PHRAP software, developed at the University of Washington (http://www.genome.washington.edu/UWGC/analysistools/phrap.htm). A group of homologous proteins ("Protein Cluster V") with unknown function was chosen for further studies.
[0033] EST databases provided by the EMBL (http://www.embl.org/Services/index.html) were used to check whether the human proteins in Cluster V were expressed, in order to identify putative pseudogenes. One putative pseudogene was identified and excluded.
[0034] (a) Alignment
[0035] The human part of this protein family includes seven different 150-250 residue polypeptides shown as SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20, encoded by the nucleic acid sequences shown as SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, and 19. The amino acid sequence shown as SEQ ID NO: 2 was identified to correspond to a human 261 aa sequence encoded by the gene "WUGSC: H_DJ0747G18.5" (GenBank Accession No. AC004876). No function has been associated with the said gene.
[0036] An alignment of the human polypeptides included in Protein Cluster V, using the ClustalW multiple alignment software (Thompson et al. (1994) Nucleic Acid Research 22: 4673-4680) is shown in Table I. The alignment showed a high degree of conservation over a 100 residues region in the protein (corresponding to positions 23-147 in SEQ ID NO: 2), indicating the presence of a novel domain.
[0037] (b) HMM-Pfam
[0038] A HMM-Pfam search was performed on the human family members. Pfam is a large collection of protein families and domains. Pfam contains multiple protein alignments and profile-HMMs (Profile Hidden Markov Models) of these families. Profile-HMMs can be used to do sensitive database searching using statistical descriptions of a sequence family's consensus. Pfam is available on the WWW at http://pfam.wustl.edu; http://www.sanger.ac.uk/Software/Pfam; and http://www.cgr.ki.se/Pfam. The latest version (4.3) of Pfam contains 1815 families. These Pfam families match 63% of proteins in SWISS-PROT 37 and TrEMBL 9. For references to Pfam, see Bateman et al. (2000) The Pfam protein families database. Nucleic Acids Res. 28:263-266; Sonnhammer et al. (1998) Pfam: Multiple Sequence Alignments and HMM-Profiles of Protein Domains. Nucleic Acids Research, 26:322-325; Sonnhammer et al. (1997) Pfam: a Comprehensive Database of Protein Domain Families Based on Seed Alignments. Proteins 28: 405-420.
[0039] The HMM-Pfam search indicated that no previously known domains could be identified in Protein Cluster V.
[0040] (c) TM-HMM
[0041] The human proteins in Cluster V were analyzed using the TM-HMM tool available e.g. at http://www.cbs.dtu.dk/services/TMHMM-1.0. TM-HMM is a method to model and predict the location and orientation of alpha helices in membrane-spanning proteins (Sonnhammer et al. (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. ISMB 6:175-182). The results indicate that the human Cluster V proteins contain 3-4 transmembrane segments.
[0042] (d) Analysis of Non-Human Orthologs
[0043] The Caenorhabditis elegans genome includes four genes, designated K07B1.4 (GenBank Accession No. AF003384), F59A1.10 (GenBank Accession No. Z81557), Y53G8B.2 (GenBank Accession No. AC006804), and W01A11.2 (GenBank Accession No. U64852) orthologous to the human Cluster V genes. The closest ancestor (K07B1.4) is on average 44% identical to the 10 human gene products. (See also: Genome sequence of the nematode C. elegans: a platform for investigating biology; The C. elegans Sequencing Consortium. Science (1998) 282:2012-2018. Published errata appear in Science (1999) 283:35; 283:2103; and 285:1493.)
[0044] The Drosophila melanogaster genome includes four genes orthologous to human Cluster V. The most closely related genes, designated "CG1942" (GenBank Accession No. AE003840—36) and gene: "CG1946" (GenBank Accession No. AE003840—37) are 39% identical to the human gene products. (See also Adams et al. (2000) The genome sequence of Drosophila melanogaster; Science 287:2185-2195) is 42% identical to the human protein set.
[0045] The human proteins in Cluster V show 27% identity to two yeast proteins; S. Cerevisiae SCYOR245C—1 (GenBank Accession No. Z75153) and S. pombe SPCC548—1 (GenBank Accession No. AL359685). The yeast proteins are of unknown function.
[0046] The tissue distribution of the human genes was studied using the Incyte LifeSeq® database (http://www.incyte.com). The genes shown as SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17 were found to be expressed primarily in the following tissues:
[0047] SEQ ID NO: 1 and 3: Liver, digestive system
[0048] SEQ ID NO: 7 and 9: Exocrine Glands, Connective Tissue, Germ Cells
[0049] SEQ ID NO: 11: Female genitalia, urinary tract
[0050] SEQ ID NO: 17: Female genitalia, nervous system
[0051] SEQ ID NO: 13 and 15: Digestive System
[0052] SEQ ID NO: 5: Cardiovascular system
[0053] Therefore, the said nucleic acid molecules and the encoded polypeptides shown are proposed to be useful for differential identification of the tissues or cell types present in a biological sample and for diagnosis of diseases and disorders related to the tissues where the genes are expressed.
[0054] Microarrays consist of a highly ordered matrix of thousands of different DNA sequences that can be used to measure DNA and RNA variation in applications that include gene expression profiling, comparative genomics and genotyping (For recent reviews, see e.g.: Harrington et al. (2000) Monitoring gene expression using DNA microarrays. Curr. Opin. Microbiol. 3(3): 285-291; or Duggan et al. (1999) Expression profiling using cDNA Microarrays. Nature Genetics Supplement 21:10-14).
[0055] In order to investigate the mechanisms whereby β3-AR agonists affect gene regulation in adipose tissue in vivo, a study was carried out using Affymetrix GeneChip oligonucleotide arrays by comparing the transcript profiles of a large number of genes in white adipose tissue derived from C57BL/6J mice treated with the β3-AR agonist CL-316, 243, or from control mice injected with a saline solution.
[0056] PolyA+ mRNAs were extracted from white adipose tissue from control and β3-AR agonist treated mice respectively. They were reverse transcribed using a T7-tagged oligo-dT primer and double-stranded cDNAs were generated. These cDNAs were then amplified and labeled using In Vitro Transcription (IVT) with T7 RNA polymerase and biotinylated nucleotides. The populations of cRNAs obtained after IVT were purified and fragmented by heat to produce a distribution of RNA fragment sizes from approximately 35 to 200 bases. Two Affymetrix Mu 19K and Mu11K sets of 3 arrays (subA, subB and subC) and 2 arrays (subA and subB) respectively, were hybridized (using the recommended buffer) overnight at 45° C. with the control or the treated denatured samples. The arrays were then washed and stained with R-phycoerythrin streptavidin with the help of an Affymetrix fluidics station. The cartridges were scanned using a Hewlett-Packard confocal scanner and the images were analyzed with the GeneChip 3.1 software (Affymetrix).
[0057] The results indicate that the mouse gene (GenBank accession No. AA275948), orthologous to the worm gene F59A1.10, is down-regulated by β3-AR agonist treatment. It is hypothesized that the human genes in Cluster V are similarly involved in metabolically important signaling pathways.
[0058] Multiple Tissue Northern blotting (MTN) is performed to make a more thorough analysis of the expression profiles of the proteins in Cluster V. Multiple Tissue Northern (MTN™) Blots (http://www.clontech.com/mtn) are pre-made Northern blots featuring Premium Poly A+ RNA from a variety of different human, mouse, or rat tissues. MTN Blots can be used to analyze size and relative abundance of transcripts in different tissues. MTN Blots can also be used to investigate gene families and alternate splice forms and to assess cross species homology.
[0059] In order to assay for proteins interacting with Protein Cluster V, the two-hybrid screening method can be used. The two-hybrid method, first described by Fields & Song (1989) Nature 340:245-247, is a yeast-based genetic assay to detect protein-protein interactions in vivo. The method enables not only identification of interacting proteins, but also results in the immediate availability of the cloned genes for these proteins.
[0060] The two-hybrid method can be used to determine if two known proteins (i.e. proteins for which the corresponding genes have been previously cloned) interact. Another important application of the two-hybrid method is to identify previously unknown proteins that interact with a target protein by screening a two-hybrid library. For reviews, see e.g.: Chien et al. (1991) The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. U.S.A. 88:9578-9582; Bartel P L, Fields (1995) Analyzing protein-protein interactions using two-hybrid system. Methods Enzymol. 254:241-263; or Wallach et al. (1998) The yeast two-hybrid screening technique and its use in the study of protein-protein interactions in apoptosis. Curr. Opin. Immunol. 10(2): 131-136. See also http://www.clontech.com/matchmaker.
[0061] The two-hybrid method uses the restoration of transcriptional activation to indicate the interaction between two proteins. Central to this technique is the fact that many eukaryotic transcriptional activators consist of two physically discrete modular domains: the DNA-binding domain (DNA-BD) that binds to a specific promoter sequence and the activation domain (AD) that directs the RNA polymerase II complex to transcribe the gene downstream of the DNA binding site. The DNA-BD vector is used to generate a fusion of the DNA-BD and a bait protein X, and the AD vector is used to generate a fusion of the AD and another protein Y. An entire library of hybrids with the AD can also be constructed to search for new or unknown proteins that interact with the bait protein. When interaction occurs between the bait protein X and a candidate protein Y, the two functional domains, responsible for DNA binding and activation, are tethered, resulting in functional restoration of transcriptional activation. The two hybrids are cotransformed into a yeast host strain harboring reporter genes containing appropriate upstream binding sites; expression of the reporter genes then indicates interaction between a candidate protein and the target protein.
[0062] The polymerase chain reaction (PCR), which is a well-known procedure for in vitro enzymatic amplification of a specific DNA segment, can be used for direct cloning of Protein Cluster V genes. Tissue cDNA can be amplified by PCR and cloned into an appropriate plasmid and sequenced. For reviews, see e.g. Hooft van Huijsduijnen (1998) PCR-assisted cDNA cloning: a guided tour of the minefield. Biotechniques 24:390-392; Lenstra (1995) The applications of the polymerase chain reaction in the life sciences. Cellular & Molecular Biology 41:603-614; or Rashtchian (1995) Novel methods for cloning and engineering genes using the polymerase chain reaction. Current Opinion in Biotechnology 6:30-36. Various methods for generating suitable ends to facilitate the direct cloning of PCR products are given e.g. in Ausubel et al. supra (section 15.7).
[0063] In an alternative approach to isolate a cDNA clone encoding a full length protein of Protein Cluster V, a DNA fragment corresponding to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17 or 19, or a portion thereof, can be used as a probe for hybridization screening of a phage cDNA library. The DNA fragment is amplified by the polymerase chain reaction (PCR) method. The primers are preferably 10 to 25 nucleotides in length and are determined by procedures well known to those skilled in the art. A lambda phage library containing cDNAs cloned into lambda phage-vectors is plated on agar plates with E. coli host cells, and grown. Phage plaques are transferred to nylon membranes, which are hybridized with a DNA probe prepared as described above. Positive colonies are isolated from the plates. Plasmids containing cDNA are rescued from the isolated phages by standard methods. Plasmid DNA is isolated from the clones. The size of the insert is determined by digesting the plasmid with appropriate restriction enzymes. The sequence of the entire insert is determined by automated sequencing of the plasmids.
[0064] To produce proteins of Cluster V, a polypeptide-encoding nucleic acid molecule is expressed in a suitable host cell using a suitable expression vector and standard genetic engineering techniques. For example, the polypeptide-encoding sequence is subcloned into a commercial expression vector and transfected into mammalian, e.g. Chinese Hamster Ovary (CHO), cells using a standard transfection reagent. Cells stably expressing a protein are selected. Optionally, the protein may be purified from the cells using standard chromatographic techniques. To facilitate purification, antisera is raised against one or more synthetic peptide sequences that correspond to portions of the amino acid sequence, and the antisera is used to affinity purify the protein.
[0065] Methods are known in the art for elucidating the biological function or mode of action of individual genes. For instance, RNA interference (RNAi) offers a way of specifically and potently inactivating a cloned gene, and is proving a powerful tool for investigating gene function. For reviews, see e.g. Fire (1999) RNA-triggered gene silencing. Trends in Genetics 15:358-363; or Kuwabara & Coulson (2000) RNAi-prospects for a general technique for determining gene function. Parasitology Today 16:347-349. When double-stranded RNA (dsRNA) corresponding to a sense and antisense sequence of an endogenous mRNA is introduced into a cell, the cognate mRNA is degraded and the gene is silenced. This type of posttranscriptional gene silencing (PTGS) was first discovered in C. elegans (Fire et al., (1998) Nature 391:806-81 1). RNA interference has recently been used for targeting nearly 90% of predicted genes on C. elegans chromosome I (Fraser et al. (2000) Nature 408: 325-330) and 96% of predicted genes on C. elegans chromosome III (Gönczy et al. (2000) Nature 408:331-336).
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Florida citrus superhero appears in first comic
09/17/2014 3:57:30 PMAs part of a $1 million agreement with Marvel Comics, the Bartow-based Florida Department of Citrus... Watch Video.
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09/18/2014 11:31:42 AMNAPLES, Fla. — The Florida Fruit & Vegetable Association opened its 71st annual convention on... Watch Video.
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09/11/2014 11:35:46 AMNavel orange producers in California's Central Valley are expected to harvest 78 million 40-pound boxes... Read more.
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Berlin-Mitte Apartment Rental Photos and Description
You will live in a cosy, charming and light apartment in a typical Berlin building from about 1900 (fully renovated) in the heart of this prospering city. For underlining the character of the apartment the new furniture was picked very carefully so you will feel comfortable immediately. The kitchen is fully equipped with all objects new or in a top of state. As the apartment is new on this site most dates are still available.
- Suitability:
- Long-term Renters Welcome
- non smoking only
- pets not allowed
- suitable for elderly or infirm
- wheelchair inaccessible


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Cummy Kiss - Scene 4
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DVD Title Cummy Kiss
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Duration 19m, 56s
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These horny studs love to use their mouths! Long haired and submissive cocksucker Sasha is in the middle once again, using his mouth on the erect poles of virile studs Andrija and Mateya. Blond Andrija also gets in on the action, using his own mouth to get his buddies off - but it's Sasha who gets all the anal loving! His two fuck buddies take turns riding him in the ass, holding him firmly by the hips and pushing down on the small of his back for the deepest penetration possible. Of course, they keep a dick in his mouth at all times! Sasha takes one cumload in his mouth, surprising blond Andrija by spitting it down his throat!
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Find records of H Thompson
Find birth, death, marriage, military, and other records of H D Thompson at Ancestry.com.Search for records
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H D Thompson (1920 - 1994) was born on January 8, 1920. H was born into the Thompson family.
H had no-known relationships. H died on February 24, 1994 at 74 years of age.
H D Thompson's last known residence is at Santa Fe Springs, Los Angeles County, California.
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Thomas Halleck
Tech Stocks Boost S&P 500 To Historic Highs
November 26 2014 7:24 PM
The gains in the tech sector overcame a floundering energy market.
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The update will roll out to iPhone users today and Android users in the coming week.
Turkey Day Traveling: Google Maps' Top 5 Tips
November 26 2014 3:23 AM
Don't leave on Wednesday. If you must, avoid heavy traffic hours between 2 p.m. to 7 p.m.
Twitter In Talks With Selfie Social Network 'Shots'
November 26 2014 12:16 AM
More than two-thirds of Shots' users are women under the age of 24, an attractive demographic for Twitter.
App Prevents You From Drunk Dialing, Texting
November 25 2014 4:18 AM
The app also has a feature to help find your friends and helps recover your Snapchats.
BlackBerry Discounts Passport, Offers iPhone Trade-In Program
November 25 2014 3:18 AM
The company is also planning red and white versions of the business-centric behemoth.
Nexus 6 Owners Are Running Into (A Few) Problems
November 22 2014 3:56 PM
The Nexus 6 is by far Google's most expensive flagship to date, and could be the most bug-ridden.
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November 21 2014 5:32 PM
The plan reportedly calls for the unbundling of Google Search from its other products and services.
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November 20 2014 10:39 PM
The service seeks "oppositional researchers" to dig up dirt on its foes.
Google Experiments With An Ad-Free Web
November 20 2014 9:37 PM
Readers pay a few dollars to view select websites without ads.
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November 20 2014 5:37 PM
The site would pay hefty sums for "high quality" long-form journalism, and charge readers $3 per month.
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November 19 2014 8:11 PM
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November 18 2014 9:15 PM
The subscription gaming service went down earlier this month, as well.
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November 18 2014 6:12 PM
The social network will host a conference that's traditionally used as a platform to announce new Web content.
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Latest News
LaCrosse Talk
Information about and from LaCrosse Talk
WI's Do Not Call List or call (866) 966-2255
MN's Do Not Call List or call (800) 921-4110
Federal Do Not Call List or call (888) 382-1222
Charges Faced by Former Municipal Prosecutor Pete Kisken
94th Primary Today at Polls
City Council Races Will Result in Some New Faces
A few more than half of the city council seats are on the ballot today in the city of La Crosse.
Voting outcome will insure that two new faces get seated on the council this month. That's because two districts--7 and 13--lack an incumbent. In a special election for district 7, former council member Jim Bloedorn and Justin Fons are vying to replace Mike Larsen, who quit the seat abruptly in the fall. In district 13, retired La Crosse firefighter Fran Formanek and current county board supervisor, Bill Brockmiller are hoping to replace longtime council member Bernie Maney who's retiring from the seat this year. In two races, incumbent council members may struggle to hold on to their seats. In district 15, challenger Doug Happel polled significantly better than incumbent Lorraine Decker in the February primary. Incumbent district 12 candidate Bruce Ranis, likewise got significantly fewer votes than one of his February primary opponents, Sara Sullivan. In districts 11, 16 and 17, incumbents Audrey Kader, Marilyn Wigdahl and Dick Swantz face no challengers
More To Retire/Resign From Lacrosse School District
They range from kindergarten teachers to 4th grade teachers and teacher assistants. Just some of the positions that will become open in June......just in time for the new school year. The positions come from 16 resignations and retirements the Lacrosse School Board accepted last night. And as the board has accepted 59 so far this year....filling the open spots will take a little time.
Superintendent Jerry Kember says he expects between 40 and 50 retirements every year since Lacrosse does have a senior staff. And he admits he does expect more before the year's out due to the uncertainty coming out of Madison.
An upcoming recruitment fair will be attended by board members....and the question will be... does Lacrosse have what future employees want?! Kember says yes they do.
Lacrosse School Board Member Abruptly Resigns
With a year left on his term as a Lacrosse school board member, long time board member Neil Duresky, announced at the end of last night's school board meeting......that he is resigning his seat effective April 25th. Duresky did not comment as to why the decision was made and left immediately after the meeting came to a close.
Board President Christine Claire told WIZM news that an appointment to the seat will have to me made after the 25th.......and whoever is chosen would take over the position for a one year term.
Coincidentally, Duresky is resigning the same day the 3 school board members that are elected today will be sworn into office.


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Showing 385-480 of 826 items
The Warehouse Antiques & Confectionery
Antiques, primitives, collectibles, hand-dipped chocolate confections, novelty candies, fudge and more.
CITY: St. Charles
Funderburg Antiques
Large European and American furniture from 1840's to 1930's: armoires, sideboards, tables, bureaus, chairs. More than 1,000 stained and beveled glass windows, some matching. Owner makes several annual European buying trips.
CITY: Belvidere
Sam's Family Restaurant
Sam's Family Restaurant is great for any age. Come in today with your family for a cozy homecooked meal at an affordable price. Try our daily special or a signature item off our menu!
CITY: Cortland
Wok n' Fire
Contemporary setting featuring Pan Asian Cuisine, Sushi Bar and full bar/lounge area.
CITY: St. Charles
Ridgefield Antique Dealers Assc.
Several antique shops located in a quaint rural setting. All shops are within walking distance.
CITY: Crystal Lake
Primrose Lane
Antiques, home decor, one-of-a-kind architectural pieces and unique gift items.
CITY: West Dundee
Festival of Arts and Crafts - Spring
All crafts are handmade in the USA and include furniture, photography, stained glass, holiday and everyday home decor items, jewelry, doll clothes and much moore
CITY: St. Charles
Angelo's Restaurant
Complete family restaurant on the Historic Woodstock Square. Come to the Woodstock Square to enjoy the many things to see and do, then stop in to Angelo's Restaurant to enjoy a good old-fashioned family style meal that is sure to satisfy everyones taste.
CITY: Woodstock
Shop In Style
Shop In Style (SIS) brings a new flavor to your shopping experience by allowing you to finally Shop In Style. Four sisters have brought together all their favorite styles, colors and collectibles to finally deliver the boutique that comes to you. The items you can expect to see include jewelry, cosmetics, clothing and accessories that are purchased from across the United States all of which you have never seen before in your local stores. These unique items will truly make you stand out from the rest of the crowd with a touch of Flair, Fun and most of all Style! You have the option of visiting their boutique or they come to you! Call to schedule an appointment or to be added to their email blast list at 815-751-1918 or email email@example.com
CITY: Sycamore
Gina's Subs & Pizza
Home of the finest home-made soups, subs & pasta dishes, including home-made lasagnas & sauces. Now you can order online, too. Check out our website.
CITY: St. Charles
Cattywampus featuring MCR Framing
Fun and unusual gifts plus home accessories and custom framing for your favorite art.
CITY: Genoa
The Mink Barn - Furs by Talidis
Hundreds of ready-made garments. Specializing in mink but also have long hair and sheared beaver, raccoon, foxes of all shades, lynx, Russian sable.
CITY: Union
Furniture from primitives to Victorian, advertising, signs, country accessories and more. Call ahead.
CITY: Belvidere
Ginger Blossom
Ethnic and traditional crafts, rugs, furniture, sweaters, antiques and more from all over the world. Ginger Blossom buys from small-scale producers who are paid an equitable wage, work under safe conditions, and do business in an ecologically sustainable manner.
CITY: Richmond
The Country Club
Here, at The Country Club, we produce handmade candles, soaps and bath products. Our wonderful soaps, lotions and bath bombs are carried in stores nationally and locally. Our hand-raised goats provide us with milk, which is used in many of our products. We have a full line of glycerin soap by the slice, and cold process soaps, as well as silky goat milk lotion in many fantastic fragrances. Our candles are well-known, and come in a huge variety of scents. We are really just a little shop full of unique soaps, candles and bath products.
CITY: Belvidere
Days Inn - Barrington Chicago Northwest
Newly renovated, value-driven rates, weekly rates, business class rooms.
CITY: Barrington
Barnelli's Pasta Bowl
Come to Barnelli's where secret family recipes and the freshest ingredients combine to bring you the best homestyle Italian cooking. Including favorites such as gourmet salads, award winning ribs, homemade pasta dishes, great sandwiches, and much more.
CITY: Crystal Lake
Royal Victorian Manor B&B
Royal Victorian Manor Bed and Breakfast is an 1894 Victorian home that has evolved to offer the perfect combination of modern amenities while maintaining its original majestic qualities. Featured as the "The Cherry Street Inn" in the movie "Groundhog Day" (1992), this majestic painted lady is designed to cater to the discerning traveler. Located in Woodstock, Illinois, the Manor is celebrated as a historic and cultural vacation retreat.
CITY: Woodstock
Cafe 72
Cafe 72 is a family owned and run restaurant serving daily breakfast, lunch and dinner specials. A party room to accommodate up to 75 guests, free of charge when ordering off the menu.
CITY: Genoa
Walcamp Outdoor Ministries Family Campground
Walcamp Outdoor Ministries Family Camp would like to invite you and your family to come camping with us! Can host family reunions, company or church picnics, and many themed weekends! Situated on 177 acres of woods, fields, streams and ponds!
CITY: Kingston
Burnidge/ Paul Wolff Campground
Playground and fishing. 9 miles of hiking trails.
CITY: Elgin
Pro Golf - Crystal Lake
Come shop at the Pro Golf store for everything you need for golfing. We have top name clubs, apparel, bags, balls, shoes, and more. The perfect place to find a gift for the golfer you know.
CITY: Crystal Lake
Super 8 Motel - St. Charles
Meeting space for up to 15 people. Free coffee, juice, bagel and toast bar. Copier and fax service. Suite wtih microwave and refrigerator. Pets welcome.
CITY: St. Charles
Courtyard by Marriott - St. Charles
121 rooms, indoor pool, exercise facility. Restaurant open for breakfast only, lounge serving beer and wine.
CITY: St. Charles
Eduardo's Mexican Restaurant
Enjoy a little taste of Mexico. The only place for great Mexican food! Daily specials, children's menu, Mexican beer, private parties, full service lounge.
CITY: DeKalb
Arte Verde Garden Center and Landscape Co.
Come see the area's largest selection of top-quality plants, including annuals, perennials, hanging baskets, vegetable plants, and shrubs. Farm animals and exotic birds. Unique plants not found in other garden centers. A name that stands for quality!
CITY: Poplar Grove
Karlsbad Tavern
Karlsbad constantly has specials and a changing menu. Every Friday night is our famous Fish Fry, and every Sunday we have biscuits and gravy starting at 10am. We are a small tavern located in Downtown Genoa. Come in for good food, friendly service, and a nice conversation. Kids are welcome.
CITY: Genoa
Angelic Organics Learning Center
We offer public and custom programs for children, adults, and families on our beautiful, organic farm to help build the local food system. Whether its a day camp or a cheese making workshop, participants gain hands-on experience preparing farm-fresh food, from field to table, while learning about organic agriculture and the whole farm system in the process. The Learning Center also offers farmer training and support to beginning farmers and facilitates youth-led urban agriculture projects in Chicago and Rockford.
CITY: Caledonia
Hilton Garden Inn- St. Charles
120 room full service hotel and corporate training center offering state-of-the-art technology. Deluxe accommodations, fitness center, indoor pool, banquet facilites and restaurant.
CITY: St. Charles
Poc's Music
Poc's Music in DeKalb has most of your musical needs including guitars, bass guitars, amplifiers, drums, percussion, PA gear and accessories. We also rent band instruments to students and carry accessories for school bands.
CITY: DeKalb
Ruth's Chris Steak House
Featuring USDA Prime Steaks, Lobster, Fresh Fish & Seafood, and Select Creole Specialities. Award Winning Wine List. Private Dining Available.
CITY: South Barrington
Grampa's Restaurant
We specialize in Fish, Steaks, Pastas and Grampa's own special Pizza, as well as Sandwiches & Melts for your dining pleasure!
CITY: Belvidere
Chicago Northwest KOA Campground
Tent, vehicle, and cabin sites. Game room, pavilion, playground, and laundry facilities.
CITY: Union
Steel Heart Ltd.
Unique showroom & warehouse sprawls over an impressive 50,000 square foot inside the historical Starline building, which is the perfect showroom for their pieces. With the outside of the building encased with climbing ivy, huge skylights, and old wooden beams and floors, their products feel at home here. Shop a vast collection of home and garden decor featuring arbors, gazebos, trellis, planters, wall baskets, chandeliers, statuary, antiques, cast iron and more.
CITY: Harvard
Degroovia Guitars
Degroovia Guitars focuses on vintage, modern and used guitars, amplifiers, effects and more. Degroovia sells, accepts and encourages trades.
CITY: DeKalb
Kirkland Family Restaurant
A great place to meet and eat! Serving breakfast, lunch, and dinner. Our Friday fish fry begins at 11am. Join us for delicious food, friendly service, and great prices!
CITY: Kirkland
Vino Thai's
At Vino Thai, you'll discover the best of Thai cuisine, the freshness of carefully selected ingredients, the warm welcome, attentive service and an unforgettable experience.
CITY: St. Charles
Barn of Barrington
Casual or intimate gourmet dining for lunch or dinner.
CITY: Barrington
Then and Now Gift Shop
Unique and specialty items located in the Boone County Historical Society Museum.
CITY: Belvidere
Johnny's Charhouse
Come enjoy the menu, including steaks, chops, seafood, Italian specialities, and a martini bar.
CITY: Sycamore
Jane Fargo Hotel
In the tradition of a small, comfortable hotel, all of our guests feel like executives. We are located in beautiful downtown Sycamore making us within walking distance of attractions, dining, and shopping. Jane Fargo Hotel's guest rooms include, master, corner and lofted bedroom suites. Featuring whirlpool baths. Meeting rooms up to 10.
CITY: Sycamore
Long Prairie Trail
This 14.6 mile paved recreational trail runs south of Illinois Route 173 and winds past the Northern Boone County towns of Caledonia, Capron, Belvidere and Poplar Grove.
CITY: Caledonia
Gardens & Gatherings
Seven minutes west of downtown St. Charles in Wasco off Rt. 64 (west Main Street). Weddings our specialty.
CITY: Wasco
Oak Hill Gardens
25,000 square feet of tropical splendor. Known for easy-to-grow orchids, flowering house plants and indoor foliage. Open year 'round.
CITY: West Dundee
Chessies Restaurant
Chessie's was built around Barrington's former train station and features a unique dining experience in a vintage Illinois Central railroad dining car. The Menu ranges from appetizers to salads, sandwiches, pasta, fresh fish, and USDA choice steaks from the Stockyards Packing Company. Daily specials including $5 Burger Day, Friday Fish Fry, Saturday Prime Rib Specials and Sunday Brunch. Our banquet facility is available for your next event for groups of 25-80.
CITY: Barrington
Sycamore Antiques
Fine art, antiques and collectibles can all be found at Sycamore Antiques. Stop in to see all that we have to offer!
CITY: Sycamore
Panda House Chinese Restaurant
Panda House serves Szechuan, Hunan and Cantonese Cuisine. They are open 7 days a week from 11:00 am to 11:00 pm Eat in or take out, they also deliver to DeKalb, Sycamore, Malta and Cortland with a minimum $8.00 order. They accept Visa, Master Card, Discover and Huskie Bucks.
CITY: DeKalb
Crystal Lake - Super 8
59 rooms available. Queen beds, newly refinished. Located near shopping center. Several restaurants within walking distance. Also near a bike path.
CITY: Crystal Lake
Taxco Restaurant Too, Inc.
Travel to Mexico without evening leaving the USA. Taxco will immerse you into the mexican culture as soon as you step through their door way starting with their authentic decor and the amazing aroma flowing from the kitchen. Taxco serves the most authentic mexican cuisine using only the freshest ingredients.
CITY: Sycamore
Lippold Park Family Golf Center
The mini putt courses have been designed with separate themes, including the handicapped accessible Sports-N-Courts with a sports theme, and Tees-N-Trees with a prairie theme.
CITY: Crystal Lake
Colonial Antique Mall and Restoration Center
150 Quality dealers in spacious 35,000 square foot mall, featuring largest furniture inventory in Northern Illinois including: Architecturals, built-ins, mantles, lamps & fixtures. PLUS: 85 showcases - jewelry, collectibles. No reproductions or crafts.
CITY: Woodstock
Wickham Interiors, Inc
A distinctive retail store offering quality decorating accessories, fine furniture and unique gifts. Its retail showroom boutique is the ideal place to discover exciting ideas for your home or business. Located in a 1912 theatre building.
CITY: Crystal Lake
Du Page Expo Center
Trade Show facility
CITY: St. Charles
Al Dorado
Al Dorado is a Mexican restaurant that is dedicated to serving great food at reasonable prices. Come in and enjoy authentic Mexican cuisine made from only the freshest ingredients. Our friendly staff are waiting for you.
CITY: DeKalb
Pizzeria Neo
An authentic Neapolitan Pizzeria offering the aroma of wood burning ovens cooking the freshest ingredients with a vibrant modern atmosphere and unique Italian wines.
CITY: St. Charles
The Family Gathering Amish Furniture
Handcrafted Amish furniture, can be custom built to your specifications. Built only by true Amish craftsmen, with every attention to detail using only solid pieces of wood. Built to last for generations to come! Onsite gourmet coffee bar.
CITY: Harvard
Antiques on Broadway
Over 7,000 square feet of fine refinished furniture, including dining room sets, bedroom sets and accessories. Also an extensive inventory of architectural items from building salvage projects.
CITY: Richmond
Johnson's Family Farm
Our farm is not a large scale operation but we are a family run operation that does its best to provide you the customer with a good product and a friendly smile when you come for a visit. We offer 12 acres of mazes, fall decorations, and pumpkins and gourdes galore! So gather up the kids, find some friends and venture out to Sycamore Illinois and start some great memories!
CITY: Sycamore
Sweet Earth
At Sweet Earth our gifts say more!. We engrave on almost everything. We have the tools and experience to help you create a unique gift. We repair both fine and costume jewelry-AND all the work is done in the store by qualified professionals. We change watch batteries and remove links. We have the largest selection of beads & jewelry making products in the area. Every year in March we host our BEAD-A-THON. For that personalized touch, we provide engraving services and plenty of engravable gifts for every occasion. We can even put your message on your own treasures, engraving on a wide variety of materials including metals, glass and crystal. We carry beautiful gift ideas from linens and home decor to toys and puppets, greeting cards and wrappings.
CITY: Sycamore
The Thai Pavilion
Proudly presenting the food of Thailand in the heart of DeKalb. We're a bit of Southeast Asia in your own small town. Everything from appetizers, salads, fried rices, to desserts.
CITY: DeKalb
Parkside Bed and Breakfast
A contemporary bed and breakfast located in the heart of historic DeKalb County. The cottage has been newly renovated and features carefully chosen furniture, decor and attention to detail. Relax into the cushy softness of the luxurious queen bed. Settle into the soft, leather recliner or love seat and watch a movie. Walk outside and enjoy the coolness of the shade from the tall Maples and explore the lovingly tended gardens. The Parkside Bed and Breakfast is the perfect destination for travelers looking for a weekend getaway or just relaxation and comfort while on an extended business stay.
CITY: DeKalb
Mediterraneo specilizes in Middle Eastern cuisine, which is vegetarian by nature. Mediterraneo does serve some meat, but most of our entrees are vegetarian, and many are even vegan friendly. Vegetarians and meat lovers can enjoy our open buffet every Sunday from 11am-3pm.
CITY: DeKalb
Milk Pail Restaurant & Entertainment Complex
Experience fresh, made-from-scratch cuisine and our warm country hospitality. Sunday Jazz Brunch, French Country Market with over 100 unique shops, weddings, mini-golf, Murder Mysteries, picnics and Bingo.
CITY: Dundee
Hanan Hall Romantica Boutique
Original fashion jewelry designs from romantic to Renaissance. Special occasion, wedding and prom, are our specialty.
CITY: West Dundee
RavenStone Castle B&B
Step back in time and delight in the ambience of a 16th century style, greystone castle. Located in rolling farm country just south of Harvard, IL. RavenStone Castle features three Medieval theme guest rooms, private baths and European-style breakfast.
CITY: Harvard
Hampton Inn - Elgin
No. 1 rated for service in the State of Illinois. The staff is committed 100% to providing you with a great experience.
CITY: Elgin
Old Towne Pub and Eatery-Wasco
Ten plasma televisions; game room; daily drink specials; beer garden; friendly, warm pub atmosphere. Family owned.
CITY: St. Charles
Donahue Furniture
Family owned -- fine furniture. 53 years of business
CITY: Woodstock
Rosita's Mexican Restaurant
Rosita's is a family owned and operated restaurant located in DeKalb. They have been serving delicious Mexican food for over 30 years. They feature a fabulous selection of mixed drinks in a warm and cozy atmosphere.
CITY: DeKalb
Anderson Candy Shop
Simply the best chocolates you can get anywhere! Consumers Digest says "One of the best kept secrets in North America!" It's cooked in copper kettles and individually hand-dipped as it has been since 1919. Locally owned family shop.
CITY: Richmond
Bank Block Antiques and More
Antiques, collectibles, furniture, floral arrangements, jewelry, glassware, made-to-order furniture and shelves, in-home help with decorating and more.
CITY: Marengo
Cabana Charleys Tiki Bar & Grill
Menu includes American with a Caribbean flare and tropical drinks served to you in a relaxing atmosphere- "Relax, You are Here".
CITY: Sycamore
Encore Clothing
Encore Clothing specializes in quality consignment clothing for men and women. We carry currently styled great condition clothing priced at about one-third of the original retail price. Whether you're interested in shopping for great deals or in consigning your fabulous clothes to make a little extra cash, we are the place for you.
CITY: DeKalb
New Game in Town
New Game in Town is a great little shop specializing in Magic the Gathering and other CCGs, Darts and Dart Supplies, Comic Books, Miniatures, Dungeons and Dragons and other RPGs. Come in and play!
CITY: DeKalb
Small Town Skate Shop
Small Town Skate Shop has all your skateboard and long board needs. They have things for both guys and gals, along with a variety of accessories. Small Town Skate Shop also carries a wide variety of brands to choose from such as toy machine, DC, Element, Vans, PlanB, etnies, Shorty's, and more!
CITY: DeKalb
Pizza Pro's
Pizza Pro's was voted "Best Pizza in DeKalb" in 2010. Stop on in for some yummy pizza, pasta, appetizers or just enjoy a refreshing drink while catching the game on one of our many televisions. In addition to a full service restaurant, we offer delivery, take-out, and catering.
CITY: DeKalb
Wok n' Fire
Contemporary setting features Pan Asian cuisine, sushi bar, and a full bar/lounge area. This is a popular spot for both visitors and residents.
CITY: St. Charles
Anderson's Coins and Collectibles
Vintage toys, memorabilia including tin wind-up toys, Star Wars, Coca-Cola, puppets, model kits, new and retired Legos. Buy or sell gold, silver, sterling flatware, jewelry and coins.
CITY: St. Charles
Dundee Manor Restaurant
Newly added addition to a great family restaurant.
CITY: East Dundee
Harms Farm and Garden Center
Visit the petting zoo and take a ride on the Pumpkin X-press. Featuring pumpkins, apples, costumes, crafts, weekend events, flowers, nursery stock, fruit, and vegetables. Christmas trees and more!
CITY: McHenry
Quality Inn
Complimentary breakfast, fitness center, high-speed internet access, leisure suites available, lots of extras!
CITY: Elgin
Lindy's Restaurant and Marina
Waterfront restaurant and sports bar with a view of Bangs Lake from each and every seat! Enjoy American Fare with a Caribbean Flare in our brand new restaurant or either of our outdoor decks. Entertainment every Saturday and Sunday. Private marina with over 100 slips available for seasonal rental. Outdoor venues available for wedding ceremonies, beach parties and company picnics.
CITY: Wauconda
Premier Interiors
Open daily. A shop of home furnishings.
CITY: St. Charles
Wild Thyme Herb Garden
Herbs, whole food, vitamins, flower essences, books, essential oils, magazines, plants, seeds and homemade soap. The store in Genoa is for people who want to get back to nature. Unique gifts. Finest quality herbal products available.
CITY: Genoa
Town House Books
Independent book store in 1853 Greek revival townhouse, housing thousands of titles for all ages.
CITY: St. Charles
Woodstock Harley Davidson
The Midwest Superstore, Woodstock Harley Davidson, where you can purchase as well as rent one of their new Harley-Davidsons for the day, week, or special events. Everything from street rods to V-rods. Many special events to enjoy too.
CITY: Woodstock
First Street Plaza
Visit the many shops located in the new development along the river. There is a large parking garage adjacent for shoppers' convenience and 2 Visitor Information Centers.
CITY: St. Charles
Kane County Flea Market May 2015
Hours: Sat. 12-5, Sun.7-4. Admission:$5 adults, children 12 and younger, free. "The Best in the Midwest or Anywhere" - Up to 1,000 dealers display and sell antiques, collectibles, & fancy junque. A country breakfast is served on Sundays
CITY: St. Charles
Cracker Jax Shop
Cracker Jax began in 1984 as a vintage clothing store and has since transformed into the magical place it is today! All senses are delighted as you shop 15 cases of new and vintage jewelry, eclectic clothing, soy candles, oils, incense, gemstones of every kind, unique gifts, stunning greeting cards, books, antique and shabby chic furniture, art and one-of-a-kind treasures. We are lovers of all things beautiful!
CITY: DeKalb
Taylor Street Pizza - Wasco
Just 7 minutes west of downtown St. Charles in Wasco. Open daily. Delivery available.
CITY: Wasco
McHenry County Fairgrounds
Site of Annual County Fair and other events.
CITY: Woodstock
Alexander's Resturant
Family restaurant with specialties such as fantastic baby back ribs that fall off the bone, charbroiled steaks & juicy prime rib that melt in your mouth, & a wide variety of glorious burgers.
CITY: Elgin
Country Church Antiques
Specializing in pine, oak, mahogany furniture. Textiles, china, and glassware.
CITY: Crystal Lake
Nature's Garden
We've been told we're "Not just the usual" garden and gift shop. We focus on Garden and Yard Art that is both functional and fun. You never know what unique, new or crafted item you might find here!
CITY: Belvidere
Castle Gardens
Garden decorations, lawn furniture, plants, flowers, shrubs and trees. Come see our selection and find just what you're looking for!
CITY: Richmond
Robbins Flowers
Full service teleflora shop. Fresh floral arrangements designed daily. Planters, floor plants, mylar balloons, fruit baskets, stuffed animals and greeting cards.
CITY: West Dundee


text:
Climate Action
Joining together to avert catastrophe.
Fixing Planet Earth: A Not-So-Modest Proposal
by Michael N. Naglerposted Jun 03, 2010
- Only a nonviolent revolution, like the one led by Gandhi, can meet the challenge of the climate crisis.
UNC Chapel Hill Coal Free by 2020
by Kaitlin Baileyposted Jun 01, 2010
- Under pressure from students to meet climate targets, a university turns to clean power.
Reducing Carbon Emissions: Seattle Has Taken the Lead
by Richard Conlinposted May 27, 2010
- Looking back on 10 years of carbon reduction efforts in Seattle.
Climate Talks End With People's Agreement
by Jim Shultzposted May 13, 2010
Debt vs. Localization: Climate Justice in the New Economy
by David Kortenposted May 12, 2010
- David Korten: Where does the concept of "climate debt" fit into a New Economy framework?
Cochabamba's Message: Let the People Speak
by Janet Redmanposted Apr 29, 2010
- The World People's Summit broke new ground by allowing those most affected by a changing climate the chance to speak out about their ideas for just solutions.
Bill McKibben's Message to Cochabamba
posted Apr 28, 2010
- The author addresses the thousands who gathered in Cochabamba for the World People's Summit on Climate Change and the Rights of Mother Earth.
Welcome to Eaarth
by Scott Gastposted Apr 27, 2010
- Bill McKibben's latest book explores what it'll take to live on a planet less sweet than it used to be. During a recent stop in Seattle, he described the smaller, slower, and wiser future that may be our best bet.
A Climate Summit for the Rest of Us
posted Apr 27, 2010
- Video: The Cochabamba climate summit was designed to respect the power and knowledge of world social movements and indigenous peoples.
Cochabamba Postscript: Lessons, Reflections, and the Road to Cancun
by Jason Negrón-Gonzalesposted Apr 26, 2010
- There are plenty of reasons to be excited about the alliance gathering around grassroots solutions.
Cochabamba's New Direction
by Jason Negrón-Gonzalesposted Apr 23, 2010
- What did the World People's Summit accomplish, and what's next for climate justice?
The Climate's Second Chance
posted Apr 22, 2010
- Video: What is the World People's Conference on Climate Change?
The State of the Earth, 2010
by Rebecca Solnitposted Apr 22, 2010
- We're in a very bad way. But we also know the solution would make most of us richer—even if not in the ways we are presently accustomed to counting as wealth.
This is Our Home
posted Apr 22, 2010
- Slideshow: Robert Redford discusses the history and importance of Earth Day.
On the Path to Climate Neutrality
by Richard Conlinposted Apr 22, 2010
- Seattle hopes to become North America's first climate neutral city. City council president Richard Conlin asks: What exactly are we getting ourselves into?
« Previous 15 items Next 15 items » 1 ... 15 16 17 [18] 19 20 21 ... 29
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text: (TMD Remix) of Fat kid in love with himself roid head parody By themightydynasty This is the remix version of The Mighty Dynasty's Fat Kid in love with himself video... more »This is the remix version of The Mighty Dynasty's Fat Kid in love with himself video (roid head parody)... hosted and remixed King Drell. Go sign up with our network at http://www.themightydynasty.com TMD Bitches!!! « less Published March 29, 2009 530 views More Info » Full Credits themightydynasty Uploader Additional Credits Additional Credits: King Drell and Fat Kid are in the video. Stats & Data 13 Funny Votes 12 Die Votes 534 Views Published March 29, 2009 Categories: Sketch Music Parody Keywords: Fat kid In Love This Is the Remix king drell improv parody tmd The Mighty dynasty remix Love King drell spoof music roid steroid himself films head flab Bad Boy diddy funny Up Next: Grouplove - Colours AUTOPLAY Up Next In: 10 "Do Your Job" by Bruce Springsteen All Star Bowling Trick Shots That's Not Tang Senile Cat Talks Follow Facebook Twitter Tumblr Instagram Advertisement Browse Related Comments 20+ more View All ▶ themightydynasty Vaguely Related Videos Vaguely RelatedPics & Words Advertisement From Around the Web ZergNet


text:
All posts by WS Chronicle »
Atkins Class of '63 holds 50th anniversary reunion
Share The Atkins High School Class of 1963 held its 50th high school reunion from June 14–16 at the Hawthorne Inn and Conference Center and the original Atkins High (now Winston-Salem Preparatory Academy). TheRead More »
Shepherd's Center Annual Used Book Sale Was a Great Success
Share On Thursday, May 2, hundreds of avid book enthusiasts lined up at the Education Building at the Dixie Classic Fairgrounds to be able to get the wonderful bargains offered at the Shepherd's CenterRead More »
Column: Two Fathers, No Shortage of Love
Share When the daughter of close friends received her medical degree a few weekends ago, I worked long and hard to make sure our tribe of teenage boys looked sharp for the celebration. ButRead More »
Sawtooth to show Rucker's portraits
Share Local artist Leo Rucker is getting a lot of exposure as of late. The Delta Arts Center is currently hosting an exhibit of some of his murals. Next month, the Sawtooth School forRead More »
Glover named to NCCSIA team
Share Winston-Salem State University baller Justin Glover has been selected for the North Carolina Collegiate Sports Information Association's 2012-13 College Division All-State Men's Basketball Team. He is one of 10 players representing eight differentRead More »
Gone Too Soon
Share The Carl Russell Sr. Community Recreation Center and the Hanes Hosiery Recreation Center families are mourning the loss of one of their own. Celeste Burgess, 14, a longtime standout on the basketball court,Read More »
Faith leaders push for immigration bill
Share The Evangelical Immigration Table, which includes a number of faith-based groups, has launched its newest and largest paid-media campaign to reach evangelicals on the issue of immigration. The effort includes radio ads inRead More »
Exciting weekend to mark pastor's 25th anniversary
Share In celebration of the 25th pastoral anniversary of Bishop Sheldon M. McCarter, Greater Cleveland Avenue Christian Church will hold the "It's A Family Affair Weekend" from June 28–30. Events begin on Friday, JuneRead More »
Editorial: Post-racial? We don't think so!
Share It's ironic that racism – an evil that the majority on the U.S. Supreme Court seems to believe doesn't exist – revealed its ugly head in several forms this past week, colliding withRead More »


text:
Comment: Relevance? (Score 4, Insightful) 148
Someone, who has no apparent power, wants to correct a judge. Just because they think they're right and the judge had inaccurate reasoning, despite coming to the same conclusion. (There's a good XKCD comic on the subject of correcting people in the Internet.) The critic's opinion will carry no legal weight. The same critic has a history of proposing long-winded, half-baked ideas to correct issues he sees with various societal inefficiencies that have gone no-where. I'm not going to waste my time.
Would someone be so kind as to please remind me how we can block posts from a given author?


text:
shut ur fuckin mouth and stop talkin out ya ass'
someone sayin tht they have been somewhere and they havnt
by louise October 13, 2003
10 Words Trending Now
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text:
Qualified individuals who need help preparing and filing their 2012 returns can visit the Volunteer Income Tax Assistance program at Pennsylvania College of Technology.
The free service is available through early April in the Teachers' Learning Center of the Madigan Library (Room LIB/213) on Penn College's main campus in Williamsport.
Required appointments will be scheduled from 9 a.m. to 8 p.m. Monday through Thursday. Additional "first-come, first-served" dates and times are being considered for the convenience of walk-in taxpayers whose schedules cannot accommodate weekday sessions.


text:
Apple television predicted to headline three core product launches in 2013Apple is forecast by one prominent market watcher to hold three major product launches in 2013, capped off by the introduction of a full-fledged television set by the end of the year.
Gene Munster of Piper Jaffray issued a note to investors on Tuesday, aimed at calming some concern on the part of investors over Apple's next product cycle. He believes the next year will set the stage for bigger changes in Apple's lineup.
Most notably, he expects Apple will launch a television set in time for the holiday 2013 shopping season. He expects the product to be priced between $1,500 and $2,000, with screen sizes between 42 and 55 inches.
"We expect the beauty of the design to be a feature, but the most important feature will be the ability to use the TV as the main interface for the living room across multiple devices," Munster wrote. "We believe the TV will include Siri and FaceTime. The biggest item unlikely to come with the TV will be unbundled channels."
But before a television set, Munster expects Apple to hold an event around March of next year to launch a new radio service to compete with Pandora. Recent reports have revealed that Apple has been in talks with content providers to create a streaming radio service.
Munster believes that any new service would be accompanied by a new product launch. He believes the most likely product update for early 2013 could be an iPad mini with a high-resolution Retina display.
While the iPad mini just went on sale earlier this month, he believes Apple could quickly upgrade it to a high-resolution screen to "address the only significant complaint about the iPad mini."
Munster also sees Apple introducing an updated Apple TV set-top box around the same time. He believes the $99 streaming device could be updated to include a TV app store.
As for the third major product launch event in 2013, Munster believes Apple will introduce its next iPhone around September. He expects a so-called "iPhone 5S" that would be a "modest upgrade" from the current iPhone 5, with a faster processor, more RAM and a better camera.
Around that same time, he expects Apple would introduce a new full-size iPad that would feature a redesigned exterior more akin to the iPhone 5 and iPad mini.
Finally, Munster also expects Apple will preview the next versions of its Mac OS X and iOS operating systems at the annual Worldwide Developers Conference in June. He believes iOS 7 will begin to show the influence of designer Jony Ive, who took over Apple's Human Interface team last month.
"We expect iOS 7 core new features to include additional wallet features for Passbook, commerce integration for Siri (Groupon/LivingSocial as an example), and Maps improvements," he said. "We expect MacBook Airs will get a Retina display option."
Piper Jaffray has reiterated its "overweight" rating for AAPL stock, with a price target of $900.
On Topic: Future Hardware
- Apple Watch website revamped with new UI and UX details
- Next-gen iPhone rumored to sport dual-lenses, DSLR quality in 'biggest camera jump ever'
- WatchKit tools reveal Apple Watch screen resolutions, Dynamic Type support, more
- Apple iSight supplier Sony unveils new 21MP CMOS sensor with 4K video, phase detection AF
- Apple Watch chip suppliers rumored to start production soon, orders at 30M to 40M units


text:
Jeezy - RIP
Artist: Jeezy
Featuring: 2 Chainz
Producer: DJ Mustard
Album: ItsThaWorld
Avg Rating: 3.0 ( 21 votes )
Hip hop has had its fair share of songs about death. While some, like Puff's Missing You, are an ode to a fallen friend, a few others, like Eminem's aptly named Kill You or Pac's Hit Em Up, focus on the emcee taking a life themselves. On his new, 2 Chainz assisted single, R.I.P, Young Jeezy joins in on the morbidity. Aside from killing the track, Jeezy boasts about killing the club with tequila shots and Molly pills over a DJ Mustard twerk-friendly beat. The Grammy nominated Dos Chains serves as Jeezy's accomplice, adding an equally narcotics laden verse, which is sure to get you to down a few more shots. If you manage to survive Jeezy's onslaught, be sure to check out his new mixtape, El Hefe: It's Tha World, out today.
Update: Click "Watch Video" above or scroll down to view Jeezy's TAJ-directed RIP visuals.
Jeezy2 Chainz Music VideosHitmakerStreet Banger
Written by Lucas G. on 03/25/13
Submit your Rating
CTE/Def Jam/IDJMG
First DJ Booth Appearance:
"Grew Up a Screw Up" (2006)
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- Preserving Yellowstone
- CULTURE FRONT: Winter art season takes flight
- GET OUT: Desert dose before the snow
- WELL, THAT HAPPENED: Casualties of Ambition
- REDNECK PERSPECTIVE: Chisler 348 death causes outrage
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Snowpack Report: Tricky Terrain
This season's lack of snow fall has left the lower elevations thin on snowpack and the ski area terrain short on soft turns, although the skiing here is always good. The ski areas hold fast, fun turns and the backcountry still holds soft, perfect skiing on shaded, northerly aspects. When the powder hunters' hunt is on, the findings are well worth the effort.
In the backcountry strong winds continue to load slopes with active, sliding surfaces. As sun crusts and surface hoar are loaded, new snow easily releases. Even though the snow fall this season has been minimal, the existing light, density snow is easily transported by wind onto sensitive slopes. The greatest hazard still remains where the Jan. 27 to Feb. 1 snow continues lingering. Avalanches with crowns up to a three feet and greater have been reported to slide on the January drought layer. As the possibility of triggering a slide maintains moderate, the hazard of triggering a slide has increased; so check every slope.
This season's theme continues, where is the snow and when will it fall … only in December? We will never know, but can only pray for snow while the lifts are still running. And until then watch out for tricky, thin terrain.