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|Leo Paik on Jan 1, 2001
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|Easter Island Friday
|17 hours ago
|re: Moab Guide/partner ?!?!
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|3 days ago
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|Show which comments —
By Ben Kiessel
Dec 19, 2007
|Hell yes it's recommended! Climb any South face you want.
By Andrew Gram
From: Salt Lake City, UT
Dec 19, 2007
Yeah, chase the sun. I've put away the rack and skiied in the La Salles in January, and i've climbed Castleton in a sweater. You just never know.
Good routes are most anything sunny at Indian Creek or Wall Street and Kor-Ingalls on Castleton. v Most long routes will be cold.
By Indian Creek 2112
Oct 15, 2009
|I am an avid climber and love Indian creek so much I decided to by a hotel Inn at the Canyons, Monticello, Utah so I could be closer. I offer all climbers a 25% on rooms if you just mention you are a climber. My name is jared, owner of inn at the canyons and if you stay I can reccomend some amazing climbs that only locals know about. Hope to see you here. Reservations 435 587-2458
By Chalk Norris
From: Brighton, CO
May 14, 2012
|Hey 2112 I will be coming down soon like 2 weeks and was looking for a hotel. I am super excited that you are climbing friendly. Ignore buddy (joel Hicock) I really appreciate the fact that you advertised here.
By bend
Jun 22, 2012
this is a shot in the dark but i was swimming and climbing along the colorado the weekend of 15th and left a pair of 5.10 moccasins size 9.5 at the lone boulder along potash rd next to the indian writing sign :( if someone happened to find them and then look on this site that would be amazing !
thanks and i guess ill throw a reward in :)
By Drew Hallett
From: Fort Collins, Colorado
Mar 8, 2013
|i was wondering where the best place to climb pure sport routes in moab is? sadly i dont have trad gear but i want to climb while im there.
By paul bucher
From: moab, utah
Apr 3, 2013
|check out Utopia for some non gear intense routes
By Fred Knapp
May 8, 2014
High on Moab, the greatest guidebook ever to the Moab area, has been sent to the printer. You can pre-purchase a copy now and get free shipping and a 2-year eBook subscription. Written by a true Moab local, the guide covers summer crags in the LaSal Mountains, as well as the classic towers, new and old crags, bouldering, recently developed sport climbing....
100% of royalties go the Access Fund.


text:
This design is for big daddies everywhere. 'Big' is really big and 1 star is on each letter. 'Daddy' is under 'big' with a contrasting background and a cut-out outline where the color of the item will show through. There are 2 stars around 'daddy.'
This great custom product in white from our department Men and T-Shirt category can be printed just for you in the size and style you like. The design Big Daddy With Stars was created by birdy27 and uploaded to Spreadshirt for you to have printed on a T-Shirt. Next to a host of other great customized designs, you can find this Big Daddy With Stars design in Spreadshirt's Family category. If you are interested in topics like Birthday or Dad, then you'll love Big Daddy With Stars designs by birdy27. Spreadshirt also offers a huge selection of custom t-shirts, hoodies and accessories for the category Family as well as top Extra clothing. birdy27's designs have already impressed many of our customers, so we are sure that we've got right stuff for you! Spreadshirt is your destination for great designs of all kinds – whether it's Birthday, Dad or Extra. We are sure that you will find something nice in our shop, there are many amazing custom Family t-shirts, hoodies, or accessories for you to find! Tags: Design ID: 3111845
birdy27 here! thx for stopping by! got cool stuff in my WHO U BE TEES shop! of course, hundreds of designs in the Spreadshirt marketplace! hundreds of designs sold! for latest b27 designs & info, join my Facebook fan page: www.facebook.com/birdy27designs
acting, writing/songwriting, dancing, inline skating, exercise, weightlifting, fashion, makeup, movies, theater, music (r&b, jazz, hip hop, dance/remixes, latin, reggae), graphic designing, color, snorkeling, travel, vegetarianism, holistic healing/altern
Freddy Fazbear's Pizza Designed by Katsuhicon
Ash Flying Seagul T-Shirts Designed by jobby
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Damn Gina Men's T-Shirt Designed by rouzdesign
White Shark T-Shirts (Short sleeve) Designed by BreakingBizzar
This sleeveless muscle t-shirt for men has a comfortable fit with a 3/4" set-in collar and armholes. Perfect for those summer months or at the gym. Made from 100% ring-spun pre-shrunk cotton and weighs 6.1 oz. *Color gray is made from 90% cotton and 10% polyester.*
still to fill

subset: commoncrawl


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~2nd Place~
"DEBORAH the QUEEN of EVIL"
Random Oni Table Rolls
Body (37) Broad, muscular human
Head (99) Ratlike
Nose (79) Tiny nose
Eyes (22) Large round eyes that are white
Mouth (53) Large toad mouth, no lips, just a long slit with sharp teeth
Arms (46) Thin arms, gnarled, muscular, but misshapen
Legs (79) Human feet with clawed toes
Other (29) Large mane of black hair
Color (21) Light red (I think they mean pink)
Description: Deborah is a summonable demon who has the ability to cast the level 10 spell 'Uncomfortable sexual tension' between herself and any character. During battle any character afflicted with 'Uncomfortable sexual tension' will spend his turns alternating between unbearable lust and unbearable self-loathing, and has to roll a saving throw against shame-inspired suicide.
The Verdict
Zack: Second in creepiness only to that weird albino furry man thing that we also gave a prize to.
Steve: I'm just going to stare into this thing's dead eyes and try to forget we ever saw that other thing.Zack: At least this one has hooters.
Steve: But they're scary boobs. Terror boobs are the worst sort of boobs, because you should never take off a shirt and see some boobs and be like AAAAHHHH! You should be more like ahhhhhhh...
In case you read my profile online and got excited, I wanted to clear up any misunderstandings.
In a Something Awful exclusive, we reveal the true state of Darren Wilson after his harrowing encounter with Michael Brown.
It's Black Friday and here is a white rapper!
Everything I'm about to show you is art. It says nothing and is worth even less, but it's still art!
I hope you updated before the apocalypse, because the google play app store won't even connect now.
Zack Parsons, Steve "Malak" Sumner, and friends tackle bizarre role playing game products that make them wonder, "What the fuck!?" From the early days of Gygax to contemporary role playing games, none will be spared.
Copyright ©2014 Rich "Lowtax" Kyanka & Something Awful LLC.


text:
Central Wellness
Mission of Wellness Committee
The mission of the Wellness Committee is to promote healthy development of the mind, body and spirit of the members of the Central Methodist University Community through physical exercise, health and fitness awareness, educational programs, and recreational activities.
Definition of Wellness
The concept of wellness advocates a balanced approach to life and includes social, emotional, spiritual, environmental, intellectual, occupational and physical components.
Wellness Focus: November is American Diabetes Month
What is diabetes?
People who have diabetes have blood glucose (or sugar) levels that are above normal. Most of the food you eat is turned into sugar for your body to use for energy. Your body needs insulin, which is made by the pancreas, to help sugar get into your body's cells. When you have diabetes, your body either doesn't make the right amount of insulin or can't use its own insulin as well as it should. This causes sugar to build up in your blood.
What are the signs?
If you think you have diabetes, you should see your doctor right away. Some of these things may be happening to you:
- Passing urine more than normal
- Extreme thirst
- Unexplained weight loss
- Extreme hunger
- Sudden eyesight changes
- Tingling or numbness in hands or feet
- Feeling very tired much of the time
- Very dry skiin
- Sores that are slow to heal
- More infections than normal
What is the treatment?
There is no known cure for diabetes, but it can be helped with medicine and doing
things that are healthy. People with Type 1 can control it by taking insulin, eating
healthy and being active. Those with Type 2 can help their symptoms by eating healthy,
exercising and regular blood glucose testing. In both cases, self-care is important.
This means keeping tabs on your blood glucose, blood pressure and cholesterol levels.
Healthy habits every day also helps. Preventive care - like regular eye exams and
other medical checkups - can help keep your diabetes from getting worse.
Visit anthem.com for more ways to get healthy - and stay healthy.


text:
|Key Info
Modern house on the Costa Blanca, Spain
Private swimmingpool, 5 bedrooms, 3 bathrooms
Outside bbq and kitchen.
Nearby the citys of Calpé, Benidorm,Altea , Alfaz del Pi.
Only 65 away from the airport of Alicante.
Kitchen with all equipments as dishwasher, oven, freezer, fridge, microwave.
2 livingrooms with smart-TV and WiFI
|Sleeps up to 10, 5 bedrooms
|Nearest beach
|Altea, Benidorm 10 km
|Will consider
|Corporate bookings, Long term lets (over 1 month), Short breaks (1-4 days)
|Car advised
|Nearest Amenities
|1 km
|Nearest travel links
|Nearest airport: Alicante 65 km
|Family friendly
|Great for children of all ages
|No pets allowed, No smoking at this property
|Internet access, Sea view
|Central heating, Air conditioning, TV, Games room, Satellite TV, Wi-Fi available
|Kettle, Toaster, Iron, Hair dryer
|Clothes dryer, Dishwasher, Cooker, Microwave, Fridge, Freezer, Washing machine
|5 bedrooms, 3 bathrooms of which 1 Family bathrooms, 1 En suites and 1 Shower rooms, Solarium or roof terrace
|Single beds (2), Double beds (4), Cots (1), Dining seats for 10, Lounge seats for 8
|Linen provided, Towels provided, High chair
|Balcony or terrace, Private garden, BBQ
|Secure parking
This region is known for its mild climate. Ideal for people who want to spend the winter under the Spanish sun. In summer it is warm without getting too hot.
There is something for everyone: peace and quiet at the villa,
Only 8 km away from the beaches of Benidorm and Altea, 15 km from Calpé
Nice restaurants in Polop, La Nucia, Altea: details available at the villa.
Hiking, cycling and climbing in the nearby area.


text:
27" Top-Load Washer with 3.4 cu. ft. Capacity, 8 Cycles, 4 Temperatures, 700 RPM Max Spin Speed, Xtra Roll Action Plus Agitator, Fabric Sense Wash System and Quiet Spin Technology
Our Price
After Savings
29" Electric Dryer with 7.0 cu. ft. Capacity, 13 Cycles, 4 Temperature Settings, AutoDry Sensor System, Wrinkle Shield Option, Extra-Large Top-Mounted Lint Screen and Interior Drum Light
27" Top-Load Washer with 3.8 cu. ft. Capacity, 13 Cycles, 5 Temperatures, 800 RPM Max Spin Speed, Deep Clean Option, Delay Wash Option and Energy Star Qualified: White
15.5" Laundry Pedestal with Storage Drawer and Chrome Handle: Black Diamond
27" Top-Load Washer with 3.6 cu. ft. Capacity, 9 Cycles, 5 Temperatures, 700 RPM Max Spin Speed, Fabric Sense Wash System, Quiet Spin Technology and Delicate Cycle
27" Electric Dryer with 7.4 cu. ft. Capacity, 5 Temperature Settings, Wrinkle Shield Option, EcoBoost Option with Eco Monitor, Reversible Door and Ultra Quiet Noise Reduction System: White
15.5" Premium Laundry Pedestal with Chrome Handle and Storage Drawer: Lunar Silver
28" Top-Load Washer with 4.3 cu. ft. Capacity, 12 Wash Cycles, Low-Profile Impeller, Smooth Wave Stainless Steel Wash Basket and 11 Adaptive Wash Actions
15.5" Laundry Pedestal with Storage Drawer: Lunar Silver
29" Electric Dryer with 7.0 cu. ft. Capacity, 13 Drying Cycles, AccuDry Sensor Drying and Wrinkle Shield Plus
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Vice President of Human Resources Operations
Our client, is a very established company in the financial services industry relating to the mortgage sector, is seeking a qualified Vice President of Human Resources Operations. Successful candidate must be very knowledgeable regarding federal, state and local employment ...
Nov 24 -
Oklahoma City - Business & management
Lead Electrical Controls Engineer - Racine, WI
Location - Racine, WI
Relocation Expense will be Provided
Communicate with customers in writing and verbally
Evaluate and troubleshoot design problems.
Knowledge of AutoCAD Electrical the means of documenting electrical control designs is important.
Must be ...
Nov 23 -
Oklahoma City - Arch & engineering
Outdoor Proximity Marketer
Renewal by Andersen , the most recognized name in the window replacement industry, has an exciting opportunity for an Outdoor Proximity Marketers. Our Proximity Marketers contact prospective clients face-to-face and establish need and interest for our product.
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Shift Supervisor - Braum's Ice Cream - $11 - $11.75/HR
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General Manager $30k - $60k - Braum's Ice Cream
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Earn $950.00+ weekly, vent cleaning, will train, call to apply.
Earn $950.00+ weekly, vent cleaning, will train.
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Oklahoma City - Customer service
Service Manager, Kitchen Manager
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Roller for Fiberglass Company
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Oklahoma City - Medical & healthcare
Warehouse Associate 2nd Shift
Brand: Aaron'sReq# C0S008BDescription: Aaron's has a long legacy as an industry leader, with continued growth regardless of the economy. In business since 1955, we have grown toover 2,000 stores across North America built on a foundation of excellence, customer focus, ...
Outside Sales - Account Executive
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Oklahoma City - Sales & biz dev
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Maintenance/Grounds Worker
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General Laborer
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text:
Transferrin binding protein B (tbpB), an outer membrane lipoprotein, is required for the acquisition of iron from human transferrin. Two tbpB families have been documented in Neisseria meningitidis: an isotype I tbpB gene of 1.8 kb and an isotype II tbpB gene of 2.1 kb, the former expressed by meningococci in the disease-associated ST-11 clonal complex and the latter found among meningococci belonging to the hyper-invasive clonal complexes including ST-8, ST-18, ST-32, ST-41/44 as well as N. gonorrhoeae isolates. The origin of the isotype I tbpB gene is unknown, however several features in common with non-pathogenic Neisseria and the ST-11 clonal complex N. meningitidis isolate FAM18 have been documented leading to the hypothesis that the isotype I tbpB gene may also be shared between non-pathogenic Neisseria and ST-11 meningococci. As a result, the diversity of the tbpB gene was investigated in a defined collection of Neisseria species.
Two families of isotype I tbpB were identified: family A containing conserved genes belonging to ST-11 meningococci, N. polysaccharea and N. lactamica isolates and family B including more diverse isotype I tbpB genes from N. sicca, N. mucosa, N. flava, N. subflava as well as N. cinerea, N. flavescens and N. polysaccharea isolates. Three isotype II tbpB families were identified with: family C containing diverse tbpB genes belonging to N. polysaccharea, N. lactamica, N. gonorrhoeae and N. meningitidis isolates, family D including another subset of isotype II tbpB genes from N. lactamica isolates and family E solely composed of N. gonorrhoeae tbpB genes.
This study reveals another instance of similarity between meningococci of the ST-11 clonal complex and non-pathogenic Neisseria with the origin of the isotype I tbpB gene resulting from a horizontal genetic transfer event occurring between these two populations.
The genus Neisseria contains 12 species and biovars colonising humans most of which are non-pathogenic colonisers of the upper respiratory tract [1,2]. Only two species, Neisseria gonorrhoeae, the etiological agent of gonorrhoea and Neisseria meningitidis, a major cause of meningitis and septicaemia worldwide, regularly cause disease in humans [3,4].
A major determinant in the survival of Neisseria within the human host is the ability to acquire iron, the majority of which is not circulating freely in the human body but is stored in ferritin and haemoglobin or is complexed with the glycoproteins transferrin and lactoferrin [5]. Neisseria have devised ways to counteract this iron limitation through the evolution of several high-affinity receptor systems including the lactoferrin binding proteins A and B, the transferrin binding proteins A and B, and the haptoglobin-haemoglobin receptor HpuAB, each composed of an accessory lipoprotein subunit and a TonB-dependent gated porin [6-11]. In addition, Neisseria can obtain iron through the expression of the surface-exposed haemoglobin receptor HmbR [12,13].
Based on antigenic and genomic features of TbpB and tbpB, N. meningitidis isolates can be classified into two major families: isotype I (tbpB gene of 1.8 kb and TbpB protein with a mass of approximately 68 kDa) and isotype II (tbpB gene of 2.1 kb and TbpB protein with a mass of approximately 80 to 90 kDa) [14]. Isotype II tbpB genes have been documented in several N. meningitidis clonal complexes including ST-8, ST-32, ST-18 and ST-41/44 as well as non-pathogenic Neisseria [14-17]. Isotype I tbpB genes, on the other hand, have solely been identified among N. meningitidis isolates belonging to the ST-11 clonal complex and have not been detected among other Neisseria species. Four tbpB families were recently described based on partial nucleotide sequences from serogroup A clonal complex ST-4 N. meningitidis and N. lactamica isolates collected in The Gambia [16,17]. Families one and four contained diverse isotype II tbpB alleles from N. meningitidis and N. lactamica isolates and families two and three included isotype I tbpB alleles. Importantly, among the 138 isolates analysed (98 serogroup A ST-4 meningococci, 12 unrelated meningococci, 22 N. lactamica isolates, and 6 unidentified Neisseria spp.) only three isotype I tbpB alleles were found, all of which belong to meningococci [16,17].
Meningococci from the ST-11 clonal complex have been a major cause of meningococcal disease worldwide throughout the last century and despite low carriage rates continue to be associated with disease [18]. In addition to the isotype I tbpB gene, ST-11 meningococci can be distinguished from other hyper-virulent clonal complexes by the absence of an opcA gene and the possession of a class 2 porB gene [19-21]. Furthermore, similarities between the ST-11 clonal complex isolate FAM18 and non-pathogenic Neisseria spp. have been reported including the clustering of pilE sequences [22] and the comparable genetic organisation of the opcA negative locus in two N. polysaccharea isolates [23]. Taken together, these observations indicate the occurrence of specific horizontal genetic exchange events between commensal Neisseria and ST-11 meningococci which may have contributed to the described genetic isolation of this clonal complex [24]. The origin of the isotype I tbpB gene is unknown. Consequently, the distribution of the gene in a defined collection of Neisseria spp. was investigated with the hypothesis that the isotype I tbpB gene was present in other Neisseria spp.
Identification of the tbpB gene
Isotype I tbpB genes were isolated from the non-pathogenic Neisseria spp. Two families of the gene became apparent. The first contained sequences closely related to meningococcal ST-11 tbpB genes belonging to three N. polysaccharea and two N. lactamica isolates. The second included more divergent isotype I tbpB genes from the non-pathogenic Neisseria spp. N. sicca, N. mucosa, N. flava, N. subflava, N. cinerea, N. flavescens as well as from another three N. polysaccharea isolates (Table 1). Isotype II tbpB genes were obtained from the remaining six N. lactamica and two N. polysaccharea isolates, while in agreement with previous studies, N. gonorrhoeae isolates contained isotype II tbpB genes (Table 1) [25]. A further 23 non-pathogenic Neisseria isolates were analysed and found to be negative for the tbpB gene. Among these were N. polysaccharea, N. perflava, N. sicca, N. subflava, N. flava and N. mucosa isolates. These isolates may contain divergent or truncated tbpB genes unable to be amplified with the primers used, however the remainder of this study will focus on the tbpB genes that were sequenced.
Table 1. Summary of tbpB families and nomenclature used
Functional assessment of the protein was beyond the scope of the present study. Nevertheless, previously documented putative transferrin binding sites were observed based on predicted translations of the nucleotide sequences [26,27]. In particular, the highly conserved domains N3, N4 and C3, critical for efficient iron uptake and located in both the N- and C- terminal segments among isolates N. gonorrhoeae FA19, N. meningitidis M982, Moraxella catarrhalis 4223 and Acinetobacter pleuropneumoniae serotype 7, were also identified [27]. Domain N3 (residues 377 to 388 in N. gonorrhoeae FA19) displayed 100% sequence identity in both isotype I TbpB families, whereas six non-synonymous changes were observed among isotype II TbpB. Domain N4 (residues 409 to 422 in N. gonorrhoeae FA19) was also highly conserved among isotype I TbpB with the occurrence of three non-synonymous substitutions. Five variable sites were present among isotype II TbpB. Domain C3 (residues 703 to 713 in N. gonorrhoeae FA19) showed the most diversity with the occurrence of four non-synonymous substitutions among both TbpB isotypes.
Phylogenetic relationships inferred from novel Neisseria tbpB sequences
All of the sequences were aligned manually with sequences starting from and ending at the same amino acid residue in each isolate. Published isotype I and II tbpB sequences from isolates B16B6, M982, 8680, 8726, 2713, 2717 and FA19, used in previous analyses [14,16,17,27], were also included in the alignment as well as those from the sequenced genomes of N. meningitidis isolates FAM18, Z2491, MC58 and N. gonorrhoeae FA1090.
Phylogenetic analysis was undertaken using the software package ClonalFrame version 1.1, which is a statistical model-based method initially described for inferring bacterial clonal relationships using multilocus sequence data [28]. Inference is performed in a Bayesian framework and a neutral coalescent model is assumed based on the hypothesis that the bacteria in the sample come from a constant-sized population in which each bacterium is equally likely to reproduce, irrespective of its previous history. The key assumption of ClonalFrame is that recombination events introduce a constant rate of substitutions to a contiguous region of sequence with the end result that a clonal frame can be inferred. In the present study, over 50,000 iterations were performed with every hundredth tree sampled after which a 95% majority-rule consensus tree was derived. Annotation was then undertaken by importing the tree into the Molecular Evolutionary Genetics Analysis software package (MEGA version 4.0) [29].
The two major isotype families were evident with each family containing a distinct cluster of genes (Fig. 1). The shortness of the branches for isotype I tbpB genes indicated that these were a closely related group of sequences compared with the depth of the branches seen for isotype II tbpB genes where greater diversity is known to occur [30]. Closer inspection of the tree revealed the two families of isotype I tbpB genes observed by sequence analysis as well as three clusters of isotype II tbpB genes. For ease of interpretation, the two isotype I tbpB families have been named tbpBA and tbpBB with the isotype II clusters named tbpBC through to tbpBE (Fig. 1 and Table 1). This nomenclature is proposed according to published guidelines in bacterial genetics [31] and is recommended in light of the emergence of the new families revealed in this study. Hitherto, studies in tbpB genetic diversity have focussed on a specific Neisseria spp. or meningococcal clonal complex and have not encompassed all of the Neisseria species included in the present work. This inclusion has provided a more detailed analysis of tbpB diversity and will allow a more flexible nomenclature for tbpB genes.
Figure 1. Phylogenetic tree inferred from aligned tbpB genes. Over 500 trees were generated using Clonalframe from which a 95% majority-rule consensus tree was derived and imported into MEGA version 4.0 for further annotation. Meningococcal reference tbpB genes (accession numbers in brackets) belonging to isolates B16B6, M982, 2713, 2717, 8710, 8680, FA19, FA1090, FAM18, Z2491 and MC58 [Genbank: Z15129, Z15130, AJ223044, AJ279554, Y09618, Y09977, U05205, U65219, AM421808, AL157959 and AE002098, respectively] were also included in the phylogenetic analysis. The proposed nomenclature for each tbpB family is indicated by large encircled letters. The nodes 1, 2 and 3 depicted with a diamond correspond to the recombination events presented in Figure 2. Open squares denote N. gonorrhoeae tbpB sequences, open circles N. lactamica, open triangles all of the other Neisseria spp. excluding N. polysaccharea, which are depicted with black circles and N. meningitidis which are represented by black squares.
Family tbpBA was comprised of genes most closely related to those of clonal complex ST-11 meningococci with four of these belonging to N. lactamica and N. polysaccharea isolates. Family tbpBB included a divergent cluster of isotype I genes (75% identity) belonging to a variety of Neisseria spp. as well as containing a subset of N. polysaccharea isolates (Fig. 1 and Tables 1 &2).
Table 2. N. gonorrhoeae and commensal Neisseria isolates used in this study
Three distinct isotype II tbpB families were apparent (Fig 1 and Tables 1 &2). Several gonococcal genes have clustered together and can be found in family tbpBE with families tbpBC and tbpBD containing genes belonging to N. lactamica, N. polysaccharea, N. gonorrhoeae and N. meningitidis isolates. Throughout the tree isolates have clustered by Neisseria species indicative of within species conservation of tbpB genes. The Genbank accession numbers for new tbpB genes sequenced in this study are listed in Table 2 as well as those belonging to previously submitted tbpB sequences.
Genetic diversity of the tbpB genes
Genes belonging to family tbpBA were the least diverse (mean p-distance ranging from 0.001 to 0.040) with 85 polymorphic sites, the majority of which occur among N. polysaccharea and N. lactamica isolates. Overall, six fixed differences were observed between the genes of ST-11 meningococci and those of N. lactamica and N. polysaccharea with no shared polymorphisms between the two populations. Family B tbpB genes were more diverse (mean p-distance value 0.117) with 415 polymorphic sites. In a comparison of both gene families, there were 210 fixed differences and 54 shared mutations.
As expected, families tbpBC, D and E were more diverse (mean p-distances = 0.187, 0.140 and 0.112 respectively). Genes belonging to the N. polysaccharea isolates shared 99% identity with 16 segregating sites, seven of which encoded non-synonymous changes. N. lactamica tbpB genes were more diverse with 696 polymorphic sites while 829 polymorphisms were observed among N. gonorrhoeae tbpB genes. A total of 445 shared mutations were observed between N. lactamica and N. meningitidis tbpBC genes indicative of recombination, while only six were apparent between N. polysaccharea and N. meningitidis.
Very little recombination was noticeable among tbpBA genes or between these and family tbpBB, however a mosaic gene structure was present among the latter indicating that recombination occurred frequently among tbpBB genes from N. sicca, N. flava, N. subflava, N. mucosa, N. flavescens, N. cinerea and N. polysaccharea (Fig. 2a). As expected, tbpB genes from families tbpBC, D and E recombined often (Fig. 2b &2c) with most of this occurring from bases 200 to 800.
Figure 2. ClonalFrame representation of tbpB recombination events. The nucleotide sequence of tbpB genes is represented on the x axis with the red line indicating at each locus the probability for an import on a scale from 0 (bottom of the y axis) to 1 (top of the y axis). Each inferred substitution in the graph is represented by a cross, the intensity of which indicating the posterior probability for that substitution. In panel A, recombination events occurring at node 1 in the phylogenetic tree (fig. 1) are represented. A mosaic gene structure is evident with fragments present between bases 150 and 300, 800 and 1000 and 1400 and 1800. In panel B, horizontal genetic exchange at node 2 are depicted occurring from bases 200 to 800 and, in panel C node 3 is represented.
The aim of this study was to identify the origin of the isotype I tbpB gene. Previous observations have determined that these were confined to meningococci belonging to the ST-11 clonal complex [14,32]. In contrast, isotype II genes were widely distributed among N. meningitidis clonal complexes and N. lactamica isolates [14,16,17]. The results presented here reveal the existence of isotype I tbpB genes among diverse Neisseria spp. Based on phylogenetic analysis these could be divided into two families: tbpBA containing genes homologous to ST-11 meningococci and tbpBB including more distantly related isotype I genes belonging to diverse non-pathogenic Neisseria spp. (Table 1 and Fig. 1). N. lactamica and N. polysaccharea isolates were found with both tbpB isotypes while, in agreement with previous studies, N. gonorrhoeae isolates solely contained isotype II tbpB genes [25]. Phylogenetic analysis demonstrated the presence of three isotype II families, named tbpBC through to tbpBE. Family C contained genes belonging to N. lactamica, N. polysaccharea, N. meningitidis and N. gonorrhoeae isolates, family D included another subset of isotype II tbpB genes belonging to N. lactamica and N. meningitidis isolates and finally, family E comprised N. gonorrhoeae genes (Table 1 and Fig. 1). In light of the tbpB families now present a new nomenclature is proposed according to published guidelines in bacterial genetics [31]. Previously, studies in tbpB genetic diversity focussed on a specific Neisseria spp. or meningococcal clonal complex and did not encompass all of the Neisseria spp. included in the present work [14-17,25]. This inclusion has provided a more detailed analysis of tbpB diversity with the proposed nomenclature allowing more flexibility for future tbpB genes. Using this scheme genes can be grouped according to the family they belong to followed by an allele number.
A number of features are shared between clonal complex ST-11 N. meningitidis isolates and non-pathogenic Neisseria. Sequences upstream of the pilE gene from the class II pilin-producing N. meningitidis strain FAM18 were identical to the short region characterised upstream from N. polysaccharea pilE [22]. The N. polysaccharea isolate analysed (ATCC 43768) was included in the present study and harboured a tbpB gene similar to that of N. meningitidis isolate FAM18. Furthermore, opcA genes are absent among meningococci belonging the ST-11 clonal complex and were also undetectable among N. polysaccharea isolates 87043 and 90400 [19,20], which were found in this study with isotype I tbpB genes. The identification of isotype I family A and B genes among Neisseria spp. is another characteristic shared with N. meningitidis isolates belonging to clonal complex ST-11 and is indicative of the occurrence of several horizontal genetic transfer events between non-pathogenic Neisseria, in particular N. polysaccharea and meningococci belonging to this clonal complex.
The evolutionary reasons leading to the existence of two tbpB isotypes among Neisseria are unknown. However, seclusion of isotype I tbpB to ST-11 clonal complex meningococci may be due to clonal expansion or selection for this isotype. Indeed, the isotype I TbpB protein has been shown to play an essential role in iron acquisition from human transferrin with isogenic mutants deficient in TbpB failing to grow on hTf as a sole iron source [33,34]. Thus, both the TbpA and TbpB parts of the transferrin complex are critical. This was reflected in the lower diversity observed among tbpB genes belonging to families A (mean p-distance ranging from 0.001 to 0.040) and B (mean p-distance 0.117), highlighting the importance of this gene in contributing to the fitness of the organism. There has been selection for isotype I tbpB among meningococci belonging to the ST-11 clonal complex such that it has become restricted to this clonal complex. In contrast, isotype II tbpB genes have been found to provide a purely facilitating role such that TbpB-deficient mutants were only incapacitated with slower growth [34]. This has been confirmed in isogenic mutagenesis studies of both TbpA and TbpB in N. gonorrhoeae, H. influenzae and M. catarrhalis (which all contain isotype II-like tbpB genes) [35-37]. The non-essential role the isotype II tbpB gene has in iron acquisition may contribute to its hyper-variability. Indeed, Zhu et al found that the high rate of import among isotype II tbpB genes, although providing a temporary advantage because of antigenic composition, resulted in reduced fitness of the isolates [16,17]. The higher recombination patterns observed in the present study among isotype II tbpB genes (Fig. 2b &2C) combined with the deeper phylogeny seen (Fig. 1) support this.
This work investigated the distribution of the two tbpB variants among Neisseria spp. and aimed to discover the origin of the isotype I tbpB gene. Results revealed this gene was found among diverse Neisseria spp. indicating the occurrence of a horizontal genetic transfer event between N. meningitidis and non-pathogenic Neisseria. Three features shared between ST-11 meningococci and non-pathogenic Neisseria have now been described: (i) the presence of isotype I tbpB genes (ii) the identical sequences upstream of the pilE gene and (iii) the analogous genetic organisation of the opcA negative locus.
A revised nomenclature was proposed according to the published guidelines [31]. The scheme now distinguishes isotype I tbpB genes into two new families: tbpBA and tbpBB the former contained tbpB genes closely related to ST-11 clonal complex meningococci, the latter included the more distantly related tbpB genes belonging to many non-pathogenic Neisseria species. The scheme also separates isotype II tbpB genes into three new families: tbpBC comprising tbpB genes from N. meningitidis, N. lactamica, N. polysaccharea and N. gonorrhoeae isolates, tbpBD consisting of tbpB genes from N. lactamica and N. meningitidis isolates and finally, tbpBE containing N. gonorrhoeae tbpB genes.
Growth of isolates and DNA preparation
The non-pathogenic Neisseria and N. gonorrhoeae isolates used in this study are listed in Table 2. Isolates were cultured overnight on GC agar (Difco) supplemented with 1% isovitalex (Oxoid) and grown at 37°C in the presence of 10% CO2. Boiled cell suspensions were prepared for each isolate. Briefly, a PBS solution of overnight GC grown bacteria was boiled for 5 minutes, centrifuged and the supernatant stored at +4°C before being directly used for PCR.
Nucleotide sequence determination
Amplification and sequencing of tbpB genes were completed using primers listed in Table 3. Degenerate primers were used for some of the sequencing steps. PCR products were PEG purified and either sequenced directly or cloned using the TOPO PCR TA cloning kit for sequencing (Invitrogen). Nucleotide sequence determination was carried out using the Li-Cor Global IR2 system along with the Sequitherm Excel II DNA sequencing kit (ScienceTec, France). Additional sequencing was carried out by cycle sequencing with BigDye Ready Reaction Mix (Applied Biosystems) according to manufacturer's instructions and using an ABI 377 automated DNA sequencer.
Table 3. Primers used in this study
Data manipulation and analysis
The tbpB nucleotide sequences were assembled using the Staden sequence analysis package [38] and all sequences aligned manually in the Seqlab alignment program (Genetics Computer Group, Madison, Wis.). Phylogenetic analysis was undertaken using the software package ClonalFrame version 1.1, which is a statistical model-based method initially described for inferring bacterial clonal relationships using multilocus sequence data [28]. In the present study, over 50,000 iterations were performed with every hundredth tree sampled after which a 95% majority-rule consensus tree was derived. Annotation was then undertaken by importing the tree into the Molecular Evolutionary Genetics Analysis software package (MEGA version 4.0) [29].
The level of sequence diversity between tbpB genes was assessed by calculating p-distances within each tbpB family revealing the proportion (p) of nucleotide sites at which sequences differed. These analyses were conducted using MEGA. The number of fixed differences and shared polymorphisms were obtained using the software DnaSP [39]. Old and new accession numbers for tbpB genes are listed in table 2.
Authors' contributions
OBH participated in the planning of this study, performed all experimental work, data analysis and drafted the manuscript. MCJM participated in writing the manuscript. BR participated in the planning of this study, coordinated the study and assisted in writing the manuscript.
This work was funded by a Marie Curie fellowship provided by the European Commission and the Human Potential programme. The authors would like to thank D. Caugant (NIPH Norway), P. Kriz (NIPH Czech Republic), C. Ferreirós (Universidad de Santiago, Spain), JM. Alonso and MK. Taha (The Pasteur Institute, France), Y. Qvarnström (Uppsala University, Sweden), N. Bilek (Imperial College, UK), R. Tsang, (National Microbiology Laboratory, Canada) and N. Saunders (University of Oxford, UK) for the kind donation of strains.
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Connects using Interloc system
59"L x 42"W x 7"H
Aquaglide Lanai Platform With Softpack
Keeps cool beverages at your fingertips
One large storage chamber
Top zippered compartment for small items
Comfortable lower back support
Aquaglide Airport Softpack
Holds up to 33 quarts of cool beverages
26-gauge PVC w/600D polyester cover
84"L x 30"W x 14"H
Aquaglide Rockit
Unlimited fun for up to 8 people
Enjoy by standing or sitting onboard
Provides hours of fun-filled excitement
Lots of stations to rock and roll it
Aquaglide Freefall Supreme Water Slide
Giant play station for the water
Climbing ladder and huge slide
Mesh safety guard provides ample shade
Interior space with large splash zone
Connect accessories with Interloc system
Aquaglide Challenge Circuit 1
Wonderful setup for commercial use
Easy boarding with SwimStep XL platform
Tango bouncer for kids and adults
Foxtrot offers fun balancing challenges
Multiple activities available
Aquaglide Platinum Rebound Aquapark Bouncer Sets
Rebound Aquapark Bouncer Sets Include:
Your choice of 12 or 16 Bouncer
30" wide slide
10 Log
4 x 4 Boarding platform
Aquaglide Platinum Blast II Air Bag
Blast friends, relatives, and kids into the air
Big-time thrills and splashes
Hot air welded from 28-oz. 1000-denier PVC
12L x 5.5W x 36"H
Aquaglide Summit Express
16 tall mountain
Climbing wall with resting steps
Huge cave
25.5L x 12W x 16H


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Search: Bruce SpringsteenSearch profiles »
HayaomaiMatch % Unknown Join to find out!
Hallo! Ich Suche jemanden zum kennenzulernen. Gut wäre, wenn du nicht rauchst. Ich mag eher ruhige Umgebungen und Unternehmungen. Entsprechend arbeite ich in einem technischen akademischen Beruf (Ingenieur). Haustiere mag ich nicht (Katzen wären noch ok) - Kinder wären mir...
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Bruce Frederick Joseph Springsteen (born September 23, 1949) is an American singer-songwriter and musician best known for his work with the E Street Band. Nicknamed "The Boss", Springsteen is widely known for his brand of heartland rock, poetic lyrics, Americana sentiments centered on his native New
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Truth be told, I follow the Gospel According to Bruce Springsteen .
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Music: Bruce Springsteen , The National, Cobra Starship, The Ramones, The Runaways, Bruce Springsteen, Kate Rusby , Ke$ha, Miles Davis, David Bowie, Bruce Springsteen, Kanye West , Bey
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Music: Todd Snider, Warren Zevon, Derek Trucks Band, Rustic Overtones, Bruce Springsteen, Jesus H.
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The Bruce.Springsteen Test
Hopefully good test about the Boss :)
The Springsteen Test
This is a for fun test to see how much you know about the music written and performed by the greatest rocker to come out of the Garden State -- Bruce ...
The Springsteen Knowledge Test
Welcome to the Springsteen Knowledge Test. I'll be using the incredibly advanced algorithm of "number of questions you answer correctly" to let you kn...
The Music Guru Test
Hi! And welcome to my Music Knowledge Test. This test will consist of a few types of questions, each in its own section, that will test your knowledge...
The Sorta-Kinda Tough Rock & Pop Trivia Quiz
Wasn't sure if there were enough music-related trivia quizzes so I figured I'd help out. Hope you like multiple choice!
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Bafana have qualified for AFCON 2015. How will they fare at the tournament? Have your say on our Forum!
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Zurich - Spain will begin their defence of the European Championship as the world's top-ranked team, while Germany lost their No 2 slot to Uruguay in FIFA's latest standings.
Germany fell to No 3, ahead of their Euro 2012 group opponent the Netherlands. Group B rivals Portugal plummeted from No 5 to 10th.
Brazil and England each rose one place to No 5 and 6, respectively. Argentina climbed two to No 7.
Bafana Bafana have dropped to 68th from 67th last month.
Croatia, who play Spain in Group D, remained at No 8.
Denmark rose one to No 9, completing a Group B line-up of elite top-10 teams.
Ivory Coast lead African nations at No 16.
Mexico heads CONCACAF countries, up one to No 19. The United States climb one place to No 28.
Japan are now Asia's best, rising seven spots to No 23. Australia are at No 24.
Follow Sport24 on Twitter and Facebook and on Mobile and Have your Say on our Forum
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Celebrity Central / Top 25 Celebs
Hef's (previous) ex offers to bring him a strong drink and a new pipe
"I love what's real and what's real is family," says the booted contestant
"I don't think I will ever [be this glamorous] the rest of my life," the latest eliminated contestant says
Kendra, Kirstie and Romeo all were vulnerable – but who went home?
"I feel more comfortable with the tango," the DWTS contestant says of her next dance
Or did low-scoring Chris Jericho – or a surprise star – get the boot?
The Dancing contestant says she's "excited/nervous" for Monday's vote
Kendra Wilkinson, Petra Nemcova and Ralph Macchio have ended up in the bottom three
Despite earning a low score on Monday, "I give myself a 10 for how good I felt," she says
The Dancing contestant says the judge's comment about elegance "eats away at my ego"
The Dancing with the Stars contestant reveals what went wrong with her Viennese waltz


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- 10/14/14 /
- Comment
A Brief History of George Clooney Apologizing for Being a Bad Batman
Clooney's guilt over Batman & Robin has lasted 17 years.
Skip to content, or skip to search.
Clooney's guilt over Batman & Robin has lasted 17 years.
To Tomorrowland ... and beyond!
The future is
now tomorrow.
Love is real.
What was the guitarist from Toto doing there?
Sorry, ladies and gentlemen.
From TV journeyman to America's heartthrob in just 12 months.
David Gordon Green to direct.
It's called Hail Caesar.
Now you know.
This George Clooney—directed World War II movie is charming and slight.
From taking pictures of his genitals, to taking a crap in cat litter, to making Matt Damon think he's fat.
Congrats to Julia Roberts.
Now with Ocean's Eleven music.
"We're all like 50 years old, and we beat them three straight."
At 52, George Clooney is a year older than Wilford Brimley was when Cocoon was released.
CGI demands and other junk.
Until early 2014.
Killin' Nazis, but not in a fun Brad Pitt way.
He only offered an idea for part of it.
Would you rank the spinning before or after being trapped on the platform? Hard choices.
Which celebrities want to go into space?
On one level, Gravity is nothing new. But then there's the part that's—as we serious cinephiles like to say—infuckingcredible.
The movie opened the Venice Film Festival today to rave reviews.


text: ""; ?> Guitar Tabs, Guitar Chords and Lyrics - Chordie guitar tabs, guitar chords and lyrics Artists Songs Public booksPublic My songbookSongbook ResourcesDiv Forum JR's Xmas Selections you rated this: Currently 1/5 Stars. Currently 1/5 Stars. 1 2 3 4 5 average: 1 by jrob223It's Christmas Sing-a-long Time! Just the favorites...Angels We Have Heard (Misc. Christmas)Deck The Halls (Misc. Christmas)Feliz Navida (Misc. Christmas)Grandma Got Run Over (Misc. Christmas)have yourself a merry (Misc. Christmas)Joy To The World (Misc. Christmas)Let It Snow! Let It Snow! Let It Snow! ((Sammy Cahn, J.Styne (C) 1945))Rudolph The Red Nose Reindeer (Misc. Christmas)Santa Claus Is Coming To Town (Misc. Christmas)Silent night (Misc. Christmas)Silver Bells (Misc. Christmas)Silver Bells (Misc. Christmas)Up On The Housetop (Misc. Christmas)We Wish You A Merry Christmas (Traditional)White Christmas (Bing Cosby)winter wonder land (Misc. Christmas)Xmas frosty%20the%20snowman (Unknown)Xmas jingle%20bells (Unknown) Note: If you log in, you can add this book to your private collection and edit the content.Return to main list play their songs - buy their music - support the artists copyright © 2011 chordie guitar chords 5 popular songs Night Moves Bob Seger Country Roads John Denver Man On The Moon REM Mr Tambourine Man Byrds Lay Lady Lay Bob Dylan New songbooksAwesome ChristmasVarious songsEasy Playing Book 1Various songsjohaninplumaslake songbookVarious songsByardVarious songs- all public songbooks -


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Sergeantsville United Methodist Church
Thursday, November 27, 2014
Open hearts, Open Minds, Open Doors
The Sergeantsville United Methodist Church is a thriving church with members
who are passionate about making a difference in their community.
Our mission at SUMC is to make disciples and provide opportunities for spiritual growth!
Looking for a place to worship?
We'd love to have you join us at either of our two weekly services on Sunday:
9:00 AM with a weekly Communion service
11:00 AM with a children's Sunday School program from September to June.
A Brand New Nursery
At our 11 a.m. worship service
for children age 5 and under
Grand Opening - November 30!!!
Join us, so that together,
we can get to know God better!
Sergeantsville United Methodist Church is in Delaware Township, Hunterdon County New Jersey,
just one mile from the Covered Bridge on Route 604.
Physical Address: 622 Rosemont Ringoes Rd
Stockton, NJ 08557
Mailing Address: P.O. Box 51
Sergeantsville, NJ 08557
Just for You...St. Baldrick's
Our Vision Statement and History of the Church
Church CalendarFaithful Friends


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Zuccotto is semi-frozen, chilled Italian dessert. It's is traditionally made in a special pumpkin-shaped mold, that's why it's called:"Zuccotto", which means "Little Pumpkin" in Italian. It's made of slices of cake and ice cream or chilled whipped cream.This one consists of semi-frozen whipped cream, with chocolate-caramel bits and coarsely chopped toasted Hazelnut. Hope you enjoy it.
Line a deep round (half-ball) freezer-safe bowl with plastic wrap, leaving some extra wrap hanging out of the sides.
Cut the cake slices diagonally to form triangles and arrange in the bowl to line it, pointed tip will be facing the centre bottom of the bowl. You might need to line the centre with cake piece if the triangles don't meet in centre of bowl.
Sprinkle little juice over cake slices.
Using electric mixture on medium speed, whip whipping cream till soft, then gradually add icing sugar, then whipping cream powder+ Vanilla extract, and continue mixing till stiff peaks are formed, about 5-7 minutes.
Fold in the chopped chocolate+ hazelnut carefully till well combined.
Pour whipping cream mixture over cake in the bowl.
Cover top with rest of cake triangles. Fold over the plastic wrap side to cover the Zuccotto. Keep in freezer for about 4 hours or overnight.
Meanwhile prepare frosting: heat whipping cream till bubble appear on side of pot. Remove from heat. Add chocolate and stir till chocolate is totally melted. Let cool then keep in fridge till cold. Take out of fridge and whip with electric mixer till stiff peaks are formed.
One hour before serving, take out open the plastic wrap and flip the Zuccotto over serving plate and remove the bowl and plastic wrap. Spread frosting over Zuccotto and sprinkle some toasted Hazelnut on top. Keep back in freezer till serving time.
Slice and serve cold.
» Metric Converter
Pinot Noir, Chardonnay and Pinot Gris are the best wines to pair with Hazelnut Zuccotto.
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We offer fresh cut bank instrument for lease/sale, such as BG, SBLC, MTN, Bank Bonds, Bank Draft, T strips and others. Leased Instruments can be obtained at minimal expense to the borrower compared to other banking options. This offer is opened to both those and corporate bodies. Description of Instrument: 1.Instrument: Bank Guarantee (BG)/SBLC 2.Total Face Value: MINIMUM 1MILLION EURO/USD - MAXIMUM 100BILLION EURO/USD 3.Issuing Bank: HSBC London or AA rated Bank in Western Europe or USA. 4.Term: One Year, One Month 5.Leasing Price: 5% of Face Value plus 0.5% Lessor agent [Closed] & X% Lessee agent (open) 6.Style of speaking: SWIFT TO SWIFT 7.Payment: WIRE TRANSFER 8.Hard Copy: Bonded Courier surrounded by 7 We are RWA ready to close leasing with any interested client in few banking days, if interested do not hesitate to contact us via banking days Our BG/SBLC Financing can help you get your project funded, loan financing by providing you with yearly renewable leased bank instruments. We work directly with issuing bank lease providers, this Instrument can be monetized on your behalf for 100% funding: For further details contact us with the below information. Contact : Khan.kamal Email: firstname.lastname@example.com Skype ID: kamal.lease Intermediaries/Consultants/Brokers are welcome to bring their clients are 100% protected. In complete confidence, we will work together for the benefits of all parties involved.
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Clay Walker Responds to Kenny Chesney's Bro-Country Comments
In a new interview, Kenny Chesney takes issue with country songs that "objectify" women, and now fellow country star Clay Walker has responded to those comments.
In a new interview, Kenny Chesney takes issue with country songs that "objectify" women, and now fellow country star Clay Walker has responded to those comments.
Jamie Lynn Spears has come a long way since she moved to Nashville with the dream of making it in country music.
Hunter Hayes has canceled a performance that was scheduled for St. Louis on Thursday (Nov. 20), citing concerns over Missouri Governor Jay Nixon declaring a state of emergency.
Just how bad was the snowstorm that pounded Western New York this week? Watch this and see for yourself.
Rachel Bradshaw says her late husband Rob Bironas was, and forever will be, a warrior. A new tattoo permanently inks what he stood for on her wrist.
Hunter Hayes is joining a Who's Who of celebrities from across all media for this year's St. Jude Thanks and Giving campaign.
This is a pooch on a mission. Watch as this dog gives its all when it comes to grabbing a tater tot just out of paw's reach on the kitchen counter.
There's nothing like being reunited with an old friend.
Watch the first 15 seconds of this Taste of Country Music Festival highlights video to learn what this party in the mountains is all about. Watch the rest because it just looks like so much fun!
You and the person you're swapping spit with are also swapping bacteria. Lots of bacteria.
It turns out that Dierks Bentley doesn't really mind if you throw up on his stage.
Naturally, most people think of Nashville as country music's mecca, but Las Vegas is quickly becoming a second home for the genre. The most recent act to take residency is Rascal Flatts, who are booked for a short run at the Joint, which is located inside the Hard Rock Hotel and Casino.


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All Things Mardi Gras: Party Like an Animal with the Krewe of Barkus and Meoux
Don't get me wrong, the Krewe of Barkus and Meoux have a good time, but their mission is serious
Don't get me wrong, the Krewe of Barkus and Meoux have a good time, but their mission is serious
The Krewe of Sobek was established in the Ark-La-Tex in 2003 and are celebrating their 10th year this year with the Krewe of Sobek Grand Bal X Friday, January 18th at the Shreveport Convention Center!
I dropped off five Mardi Gras dresses to the cleaners today and got a Dryel for the rest that can't be dry cleaned... I'm getting my nails done tonight and I plan to Mystic in the morning. Yep, Mardi Gras kicks off this Saturday and I CAN'T WAIT!!!
The kick off to the Mardi Gras season is known as 12th Night and here in the SBC, we're celebrating it Saturday, January 5th at the Bossier Civic Center hosted by yours truly! Keep checking your K945 VIP account to score tickets to 12th Night!
It's going to be a crazy night this Saturday at the Krewe of Atlas Social Club! Yes, yes, a good time will be had at the Atlas Coronation, but sadly, I must give up my tiara and sash. As of Saturday night, I will be royalty no more.
I had the honor of hosting the Krewe of Akewa Coronation last Saturday night in Minden and while I can't speak for everyone, I know I had a great time! Congrats to all of the new incoming Royalty!
The first three events of the Mardi Gras calendar kicked the season off with a bang as we begin to welcome our new Royal Courts. Congratulations to the Captains of Centaur, Dionysos, and Les Femmes Mystique for wonderful events and fabulous new Royalty.
Coronation season is here! It's time to crown some new royalty and get the new Mardi Gras season in gear! Check out the latest calendar of events here!
Get the full scoop here for what's going on for Mardi Gras in the SBC! Laissez les bons temps rouler!
The Shreveport/Bossier Captain of Captains Doug Kochenderfer has released an updated calendar of events for Mardi Gras festivities in the SBC! There have been several changes, so if you're Mardi Gras inclined, def check this out!
Captain of Captains Doug Kochenderfer has been busy and has compiled the latest list of Mardi Gras activities for Krewes in the SBC! YES! We Mardi Party ALL year long!
Curious about the origins of Mardi Gras. The booze, the bead throwing, the boob showing? Well this video doesn't cover any of that. But it does give history of how it got started. Which is nice.


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Enochian sigils on your ribs will keep angels from finding you. (Inspired by the tv show 'Supernatural.')
This great custom product in white from our department Women and T-Shirt category can be printed just for you in the size and style you like. The design Black Enochian Ribs was created by SoupGnats and uploaded to Spreadshirt for you to have printed on a T-Shirt. Next to a host of other great customized designs, you can find this Black Enochian Ribs design in Spreadshirt's Symbols category. If you are interested in topics like Bones or Rib, then you'll love Black Enochian Ribs designs by SoupGnats. Spreadshirt also offers a huge selection of custom t-shirts, hoodies and accessories for the category Symbols as well as top angel clothing. SoupGnats's designs have already impressed many of our customers, so we are sure that we've got right stuff for you! Spreadshirt is your destination for great designs of all kinds – whether it's Bones, Rib or angel. We are sure that you will find something nice in our shop, there are many amazing custom Symbols t-shirts, hoodies, or accessories for you to find! Tags: Design ID: 10484077
Freddy Fazbear's Pizza Designed by Katsuhicon
Ash Flying Seagul T-Shirts Designed by jobby
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Winner Winner Chicken Dinner T-Shirts Designed by THATSHIRTCRAY
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White Shark T-Shirts (Short sleeve) Designed by BreakingBizzar
The relaxed fit t-shirt for women has a narrow neck rib, tailored sleeves and a redesigned cut for a better ladies fit. With plenty of extra room, this shirt is ideal for most body types. The fabric is made from 100% pre-shrunk cotton (deep heather is 50% cotton/50%polyester) and has a fabric weight of 5.4 oz.
still to fill


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Rachael Ray Oven Lovin' 5-Piece Bakeware Set
The right size. Stocking your kitchen with the 5 most-used & most-loved baking pans, this durable carbon steel collection features a long-lasting nonstick for effortless food release & boldly colored, extra wide grippy handles for oven-to-counter ease.
- Imported
- 5-piece set includes:
- 10" x 15" cookie sheet
- 9" x 13" cake pan
- 9" x 5" loaf pan
- (2) 9" round cake pans
- Durable carbon steel constructions resist warping & have a long-lasting nonstick for effortless food release & quick cleanup
- Boldly colored, extra wide grippy handles for a confident grasp
- Oven safe to 450oF
- Dishwasher safe
$100 $49.99
Up To 15% Off: THANKS
You'll really get cooking with this incredible, hard-enamel cookware set as it contains all the basic pieces to ensure you'll get your use out of each and every pan. heats quickly and evenly, reducing hot spots that can burn foods long-lasting, nonstick cooking surface allows food to release from the pan, making cleanup quick and easy sylish two-tone gradient exterior adds color and style to any kitchen handles are rubberized for comfort, dual-riveted for durability and oven safe to 350ºF cake pan oven safe to 500ºF 10-piece set includes: 1-quart saucepan with lid 2-quart saucepad with lid 3-quart sauté pan with lid 6-quart stock pot with lid 8½" skillet 10" skillet BONUS nonstick 9x13" cake pan* *When purchased online. Sorry, offer not available in store. Aluminum/porcelain/silicone. Hand wash; cake pan is dishwasher safe. Imported.
The right size. Stocking your kitchen with the 5 most-used & most-loved baking pans, this durable carbon steel collection features a long-lasting nonstick for effortless food release & boldly colored, extra wide grippy handles for oven-to-counter ease. Imported 5-piece set includes: 10" x 15" cookie sheet 9" x 13" cake pan 9" x 5" loaf pan (2) 9" round cake pans Durable carbon steel constructions resist warping & have a long-lasting nonstick for effortless food release & quick cleanup Boldly colored, extra wide grippy handles for a confident grasp Oven safe to 450oF Dishwasher safe
Rachael Ray Oven Lovin' 5-Piece Bakeware Set $100 $49.99
These handy stoneware bakers make cooking and entertaining a cinch because they can be used to bake cauliflower and ham au gratin, prep a side dish like stuffed cherry peppers or serve a refreshing cucumber salad. The contemporary design and bold color are sure to make these Bubble & Brown bakers a talking point with guests. crafted of stoneware with a glazed interior which won't react to food shallow sides result in crunchy tops for your favorite baked recipes extra wide-handles offer a confident grip oven safe to 500ºF includes 1½-quart and 2-quart round bakers Stoneware. Microwave, freezer and dishwasher safe. Quality Assurance Guarantee. Imported.
Rachael Ray Stoneware 2-pc. Bakeware Set $85 $49.99
Equip your kitchen with the baking essentials, including this 5-piece set featuring nonstick surfaces and easy-to-grasp handles. extra-wide handles and orange silicone grips for secure grasp long-lasting nonstick both inside and out oven safe to 500ºF 5-piece set includes: 10x15"cookie sheet 9x13"oblong pan 9x5"loaf pan two 9" round cake pans Aluminum. Dishwasher-safe. Imported.
Rachael Ray 5-pc. Bakeware Set $100 $49.99
This vibrant and fun square casserole dish makes your baked chicken or cheesy pasta so stylish. Use it for all of your favorite baked food or even for serving salads and slaws. crafted of stoneware with a glazed interior which won't react to food securely fitting lid seals in heat and flavors wide, comfortable handles provide a confident grip when putting the casserole in the oven or pulling it out when food is ready oven safe to 500ºF 3½-quart capacity Stoneware. Microwave, freezer and dishwasher safe. Quality Assurance Guarantee. Imported.
Provide your kitchen a multitude of pans for a variety of baking purposes with the Rachael Ray Oven Lovin' 5-Piece Bakeware Set. Use the crispy sheet pan to cook up your favorite cookies while the round pans can be used for homemade rolls. These pans have wide handles with orange silicone grips so they can be moved simply and comfortably. Includes two 9 in. round pans, a 9 in. x 13 in. rectangular pan, a 10 in. x 15 in. crispy sheet pan and a 9 in. x 15 in. deep rectangular pan Aluminum construction is long-lasting Nonstick aluminum for easy food release and simple maintenance Extra-wide handles to help provide a confident grasp Silicone grips for comfortable handling Oven safe up to 500 degrees Fahrenheit Includes exclusive Rachael Ray recipe
Delish! Prep, cook, and serve a variety of dishes with this colorful, no-fuss set of bakeware from celebrity cook Rachael Ray. Constructed with the same durable stoneware that has made her Bubble & Brown collection a bestseller--but featuring all-new items--this 11-piece set is made for how we really live. Take the bakers from oven to freezer to refrigerator to microwave, then pop them right in the dishwasher for effortless cleanup.Offering three spacious open oval bakers and two generously sized covered casseroles, your culinary endeavors are limited only by your imagination. Bake up creamy macaroni and cheese, light and healthy fish, and luscious cobblers bursting with fruit. Roast chicken to golden perfection and butternut squash to caramelized gloriousness. With the versatile au gratin bakers, you can cook individual portions and warm dips, serve sauces, and plate appetizers and finger foods. Thanks to the bakers' glazed interiors and shallow sides, food cooks evenly and completely, so it browns beautifully and stays delectably moist.To serve up the goodness, simply grab hold of the extra-wide handles with pot holders and set your stoneware down on the pair of included silicone trivets. With your choice of vibrant colors and contemporary design, you'll never feel the need to transfer your creation to a separate serving dish, even during the holidays. And when everyone's bellies are full and dinner is all said and done, the pieces nest together for space-saving storage.From Rachael Ray.
Rachael Ray Bubble & Brown 11-Piece Bakeware Set $121 $109.96
Baker's delight over this Rachael Ray Oven Lovin' nonstick bakeware set. A portion of the proceeds from this purchase will be donated to Rachael Ray's charity, Yum-o!®, which was created to empower kids and their families to develop healthy relationships with food and cooking. Nonstick coating lends easy food release and cleanup. Extra-wide handles and silicone grips give you a secure hold on your baked creations. 5-piece set Set includes: 10" x 15" cookie sheet,9" x 13" cake pan, 5"x 9" loaf pan & two 9-in. round cake pans Dishwasher and oven safe to 500°F Manufacturer's quality assurance guarantee
Rachael Ray oven lovin' 5-pc. nonstick bakeware set $99.99 $64.99
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Originally written on Pro Sports Daily | Last updated 11/9/14
The trade -- outfielder Melky Cabrera to the San Francisco Giants for left-hander Jonathan Sanchez and minor league lefty Ryan Verdugo -- seemingly made sense for the Kansas City Royals when it was announced last Nov. 7. Sure, Cabrera was coming off a career year in which he batted .305 with 44 doubles and 201 hits. However, the Royals needed starting pitching, and Sanchez had thrown a no-hitter in 2009, then gone 13-9 with a 3.07 ERA in 2010, pitching in the World Series that October before slumping slightly in 2011. "Based on all the information we had at the time and where we were economically and what we needed to do going forward in opening up an opportunity for Lorenzo Cain to play every day, it was the right baseball move for our team," Royals general manager Dayton Moore said Tuesday. "We were a better team on balance and on paper when we made that deal." After Tuesday, the trade officially can be ruled a train wreck. Sanchez was designated for assi...
Original Story: http://www.prosportsdaily.com/article...
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Version 2.1 and Version 5.0:
and for interests sake platters out of some old hard-drives. (vernier caliper is showing 3.5inches):
[ Company Website ] R.A.M. Network Security Services Website
[ Company Twitter ] R.A.M. Network Security Services Twitter
[ Company LinkedIn ] R.A.M. Network Security Services LinkedIn
[ Company Facebook ] R.A.M. Network Security Services Facebook
R.A.M Network Security Services encompasses a broad range of security-testing methods known as Ethical Hacking. It is the practice of assessing the security posture of a given security system, using the same tools and techniques employed by blackhat-hackers on a daily basis. These techniques can be performed against system Operating Systems, services, software and website-applications. There are a number of ways to achieve various results, according to your business needs.
The benefits of a comprehensive analysis can range from reducing the likely hood of downtime and financial or other business implications and damage to electronic assets.
According to the currently available statistics at the time of writing, the document "Net Losses: Estimating the Global Cost of Cybercrime Economic impact of cybercrime II Center for Strategic and international Studies – June 2014" by the renowned global Security company, McAffee, estimates that 0.14% of South Africa's GPD is lost through cyber-crime. This equates to just less than five (R4.91 Billion) Billion Rand that is stolen. Additionally South Africa ranks number six amongst the most targeted counties for cyber-crime globally by the U.S. Federal Bureau of Investigation (FBI), whilst another renowned security company, Symantec, listed South Africa as the third worst hit country globally by cyber-crime.
Here is a basic trainer for Assassins Creed 4 – Black Sails.
The game, although fun, is full of annoying sneaking missions. This trainer can assist here.
By searching for a specific array of bytes that represent a compare and a jump and replacing them with no-operation commands a sort of invisibility hack is created. By searching for an array of bytes and not relying on fixed addresses it is possible for this trainer to work on multiple versions, if not all versions.
The array of bytes to search for is: 0x80, 0x7d, 0xff, 0x00, 0x74, 0x0a, 0x5e
This should be replaced with six NOP's.
The Address in my version of the game is 0x018a239b
Image of memory before patching:
Image of memory after patching:
I have attached source code to automatically patch it.
The trainer is designed as a launcher, and as such must be placed within the games root directory and then executed. The trainer will then launch Assassins creed, whilst searching for the relevant arrays to patch. The hack is completed once a call to WriteProcessMemory is made and six NOP's are written to the address determined through pattern-searching.
Image of the trainer/launcher is displayed below:
This application is a rewrite of my original fuzzer. Several features have been added as well as a full user-friendly GUI.
Additionally the major change in this version is the ability to modify and add the attack strings through the provided sample list. This adds to the overall flexibility of the application.
Additionally the application now supports both POST and GET requests.
The provided text file "attackstrings.txt" contains a list of attack request strings, this file must reside in the applications root directory.
This can also be used to determine whether vulnerability "CVE-2007-1036 JBoss JMX-Console Access Vulnerability" exists on a particular server.
You can try for yourself a target with google, and a google dork: inurl: /reports/rwservlet/help?
As mentioned the contents can be modified to suit the attackers needs.
The sample contents of the "attackstrings.txt" file are as below:
The application and code can be downloaded and viewed below:
[ .TXT C-Code ] OraFuzz_V2_GUI.c
[ .TXT C-Code ] fuzzer.c
[ .TXT C-Code ] OraFuzz_V2_GUI.h
[ .ZIP ] VS Project Files
[ .ZIP/.EXE ] Executable File
[ .TXT ] Attackstrings.txt
SMARTERMAIL 12.0.x FREE EDITION:
Vulnerability 1:
Contact Book XSS:
It is possible to send someone a vulnerable .vcf contact file, assuming they are accessing it through Smartermail. The code is executed upon viewing the contact book.
Using the following .VCF file parameters including attack command, in javascript,
The following was the result of the above XSS attack:
Version 5.5 Enterprise:
The below fields can be seen below:
FN:foo1 foo2 foo3 foo4
The result can be seen below by visiting the contacts page, the code is executed:
The VCARDS used in these attacks can be viewed here:
[ .TXT ] VCard Version 12.0
[ .TXT ] VCard Version 5.5 Enterprise
This issue was reported to Smartertools and I am happy to report that the issues have been resolved speedily with a new build with version numbers after and including: 12.0.5197.19984
Heres some brief code that allows you to enumerate and scan for wireless ap's in your vicinity.
The data is stored both printed on screen and stored in a log file for easy viewing.


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4D Concepts Espresso Bathroom Storage Tower with HamperItem # HN-FDC077
- 2 decorative louvered formed doors
- The top door opens to 4 adjustable shelves
- The left side of the unit comes with 4 adjustable shelves
- The bottom door pivots open to a chrome metal rack
- Rack's canvas liner works as clothes hamper
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Reviewed by 3 customers
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Comments about 4D Concepts Espresso Bathroom Storage Tower with Hamper:
I ordered this for my new bathroom on september 3rd, 2012 On September 5th, I get an e-mail telling me it's not available till November the 8th, 2012 more than two months from now!! Really??? Why is it on your website if you don't have it? Stupid and you've lost a customer forever. No wonder it was cheaper on your web site, you don't have it!!!
- Attractive Molding
- Deep Interior
- Easy to Install
- Good Finish
- Poorly Crafted
Best Uses
- Decorative
- Small Spaces
Comments about 4D Concepts Espresso Bathroom Storage Tower with Hamper:
Just perfect for the bathroom! It is simple and easy to use.
Best Uses
Comments about 4D Concepts Espresso Bathroom Storage Tower with Hamper:
This piece was exactly what I was looking for. I needed a hamper that looked like a piece of furniture for a hallway outside my bathroom. The hamper holds much more than I expected! A little tedious to assemble but the care in packaging was great. This experience has made me more likely to purchase online again.
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