text:
Are you Takao Koeduka?Claim your profile
Publications (23)82.52 Total impact
- [Show abstract] [Hide abstract]
ABSTRACT: Plant specialized metabolites play important roles in human life. These metabolites, however, are often produced in small amounts in particular plant species. Moreover, some of these species are endangered in their natural habitats, thus further limiting the availability of some plant specialized metabolites. Microbial production of these compounds may circumvent this problem. Considerable progress has been made in the microbial production of various plant specialized metabolites over the past decade. Now, the microbial production of these compounds is becoming robust, fine-tuned, and commercially relevant systems using the methods of synthetic biology. This review describes the progress of microbial production of plant specialized metabolites, including phenylpropanoids, flavonoids, stilbenoids, diarylheptanoids, phenylbutanoids, terpenoids, and alkaloids, and discusses future challenges in this field.Plant Biotechnology 01/2015; · 0.94 Impact Factor
Article: Molecular Cloning and Characterization of a GDP-specific Aromatic Prenyltransferase from Citrus limon.[Show abstract] [Hide abstract]
ABSTRACT: Prenyl residues confer divergent biological activities such as anti-pathogenic and anti-herbivorous activities on phenolic compounds, including flavonoids, coumarins, and xanthons. To date, about 1,000 prenylated phenolics have been isolated, with these compounds containing various prenyl residues. However, all currently described plant prenyltransferases (PTs) have been shown specific for dimethylallyl diphosphate (DMADP) as the prenyl donor, while most of the cDNAs encoding these genes have been isolated from the Leguminosae. In this study we describe the identification of a novel PT gene from Citrus limon, Cl-PT1, belonging to the homogentisate PT family. This gene encodes a PT that differs from other known PTs, including flavonoid-specific PTs, in polypeptide sequence. This membrane-bound enzyme was specific for geranyl diphosphate (GDP) as the prenyl donor and coumarin as the prenyl acceptor. Moreover, the gene product was targeted to plastid in plant cells. To our knowledge, this is the novel aromatic PT specific to GDP from citrus species.Plant physiology. 07/2014;
Article: A coumarin-specific prenyltransferase catalyzes the crucial biosynthetic reaction for furanocoumarin formation in parsley.[Show abstract] [Hide abstract]
ABSTRACT: Furanocoumarins constitute a subfamily of coumarin compounds with important defense properties against pathogens and insects, as well as allelopathic functions in plants. Furanocoumarins are divided into two subgroups according to the alignment of the furan ring with the lactone structure; linear psoralen and angular angelicin derivatives. Determination of furanocoumarin type is based on the prenylation position of the common precursor to all furanocoumarins, umbelliferone, at C6 or C8, which gives rise to psoralen or angelicin derivatives, respectively. Here, we identified a membrane-bound prenyltransferase PcPT from parsley (Petroselinum crispum), and characterized the properties of the gene product. PcPT expression in different parsley tissues is increased by UV irradiation with a concomitant increase in furanocoumarin production. This enzyme has strict substrate specificity towards umbelliferone and dimethylallyl diphosphate, and a strong preference for the C6-position of the prenylated product (demethylsuberosin), leading to linear furanocoumarins. The C8-prenylated derivative (osthenol) is also formed, but to a much lesser extent. PcPT protein is targeted to the plastids in planta. Introduction of this PcPT in a coumarin-producing plant Ruta graveolens showed an increased consumption of endogenous umbelliferone. Expression of PcPT and a 4-coumaroyl-CoA 2'-hydroxylase gene in Nicotiana benthamiana, which does not produce furanocoumarins, resulted in demethylsuberosin formation, indicating that furanocoumarin production may be reconstructed in a metabolic engineering approach. These results demonstrate that a single prenyltransferase, such as PcPT, opens the path to linear furanocoumarins in parsley, but might also catalyse the synthesis of osthenol, the first intermediate committed to the angular furanocoumarin pathway, in other plants. This article is protected by copyright. All rights reserved.The Plant Journal 12/2013; · 6.58 Impact Factor
Article: Bioactivity of natural O-prenylated phenylpropenes from Illicium anisatum leaves and their derivatives against spider mites and fungal pathogens.[Show abstract] [Hide abstract]
ABSTRACT: A variety of volatile phenylpropenes, C6 -C3 compounds are widely distributed in the plant kingdom, whereas prenylated phenylpropenes are limited to a few plant species. In this study, we analysed the volatile profiles from Illicium anisatum leaves and identified two O-prenylated phenylpropenes, 4-allyl-2-methoxy-1-[(3-methylbut-2-en-1-yl)oxy]benzene [O-dimethylallyleugenol (9)] and 5-allyl-1,3-dimethoxy-2-(3-methylbut-2-en-1-yl)oxy]benzene [O-dimethylallyl-6-methoxyeugenol (11)] as major constituents. The structure-activity relationship of a series of eugenol derivatives showed that specific phenylpropenes, including eugenol (1), isoeugenol (2) and 6-methoxyeugenol (6), with a phenolic hydroxy group had antifungal activity for a fungal pathogen, whereas guaiacol, a simple phenolic compound, and allylbenzene had no such activity. The eugenol derivatives that exhibited antifungal activity, in turn, had no significant toxicant property for mite oviposition. Interestingly, O-dimethylallyleugenol (9) in which the phenolic oxygen was masked with a dimethylallyl group exhibited a specific, potent oviposition deterrent activity for mites. The sharp contrast in structural requirements of phenylpropenes suggested distinct mechanisms underlying the two biological activities and the importance of a phenolic hydroxy group and its dimethylallylation for the structure-based design of new functional properties of phenylpropenes.Plant Biology 07/2013; · 2.32 Impact Factor
Article: Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering[Show abstract] [Hide abstract]
ABSTRACT: Eugenol, a volatile phenylpropene found in many plant species, exhibits antibacterial and acaricidal activities. This study attempted to modify the production of eugenol and its glycosides by introducing petunia coniferyl alcohol acetyltransferase (PhCFAT) and eugenol synthase (PhEGS) into hybrid aspen. Gas chromatography analyses revealed that wild-type hybrid aspen produced small amount of eugenol in leaves. The heterologous overexpression of PhCFAT alone resulted in up to 7-fold higher eugenol levels and up to 22-fold eugenol glycoside levels in leaves of transgenic aspen plants. The overexpression of PhEGS alone resulted in a subtle increase in either eugenol or eugenol glycosides, and the overexpression of both PhCFAT and PhEGS resulted in significant increases in the levels of both eugenol and eugenol glycosides which were nonetheless lower than the increases seen with overexpression of PhCFAT alone. On the other hand, overexpression of PhCFAT in transgenic Arabidopsis and tobacco did not cause any synthesis of eugenol. These results indicate that aspen leaves, but not Arabidopsis and tobacco leaves, have a partially active pathway to eugenol that is limited by the level of CFAT activity and thus the flux of this pathway can be increased by the introduction of a single heterologous gene.Biochemical and Biophysical Research Communications 06/2013; 436(1):73-78. · 2.28 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Coumarins, a large group of polyphenols, play important roles in the defense mechanisms of plants, and they also exhibit various biological activities beneficial to human health, often enhanced by prenylation. Despite the high abundance of prenylated coumarins in citrus fruits, there has been no report on coumarin-specific prenyltransferase activity in citrus. In this study, we detected both O- and C-prenyltransferase activities of coumarin substrates in a microsome fraction prepared from lemon (Citrus limon) peel, where large amounts of prenylated coumarins accumulate. Bergaptol was the most preferred substrate out of various coumarin derivatives tested, and geranyl diphosphate (GPP) was accepted exclusively as prenyl donor substrate. Further enzymatic characterization of bergaptol 5-O-geranyltransferase activity revealed its unique properties: apparent K(m) values for GPP (9 µM) and bergaptol (140 µM) and a broad divalent cation requirement. These findings provide information towards the discovery of a yet unidentified coumarin-specific prenyltransferase gene.Bioscience Biotechnology and Biochemistry 07/2012; 76(7):1389-93. · 1.27 Impact Factor
Article: Characterization of raspberry ketone/zingerone synthase, catalyzing the alpha, beta-hydrogenation of phenylbutenones in raspberry fruits.[Show abstract] [Hide abstract]
ABSTRACT: Phenylbutanone raspberry ketone, accumulating in the mature fruits of raspberry (Rubus idaeus), imparts the characteristic aroma to the fruits. Here we describe the isolation and characterization of raspberry ketone/zingerone synthase 1 (RZS1), which catalyzed the NADPH-dependent reduction of 4-hydroxybenzalacetone and 3-methoxy-4-hydroxybenzalacetone to raspberry ketone and zingerone (the latter not found in raspberry), respectively. Its apparent K(m) values for 4-hydroxybenzalacetone and NADPH were 88 μM and 202 μM, respectively. RZS1 preferred 4-hydroxybenzalacetone to 3-methoxy-4-hydroxybenzalacetone as a substrate by a factor of 1.7, and showed a 6-fold preference for 4-hydroxybenzalacetone over p-coumaraldehyde, and no activity for coniferaldehyde. Expression analysis of the RZS1 gene throughout the plant revealed that its transcript level was highest in mature fruits. We conclude that RZS1 is responsible for hydrogenation of the α,β-unsaturated double bond of phenylbutenones, the final step of the raspberry ketone biosynthesis, in the raspberry fruits.Biochemical and Biophysical Research Communications 08/2011; 412(1):104-8. · 2.28 Impact Factor
Article: Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2).[Show abstract] [Hide abstract]
ABSTRACT: Flavonoids are natural compounds found in many plants, including the important fruit crop, tomato. Prenylated flavonoids consist of a large group of compounds, which often exhibit antitumour, antibacterial and/or anti-androgen activities. In this study, we engineered the biosynthesis of prenylated flavonoids using a Streptomyces prenyltransferase HypSc (SCO7190) possessing broad-range substrate specificity, in tomato as a host plant. LC/MS/MS analysis demonstrated the generation of 3'-dimethylallyl naringenin in tomato fruits when recombinant HypSc protein was targeted to the plastids, whereas the recombinant protein hardly produced this compound in vitro. This is the first report confirming the accumulation of a prenylated flavonoid using a bacterial prenyltransferase in transgenic plants, and our results suggest that the product specificities of prenyltransferases can be significantly influenced by the host plant.Plant Biology 03/2011; 13(2):411-5. · 2.32 Impact Factor
Article: The lack of floral synthesis and emission of isoeugenol in Petunia axillaris subsp. parodii is due to a mutation in the isoeugenol synthase gene.[Show abstract] [Hide abstract]
ABSTRACT: Floral scent has been extensively investigated in plants of the South American genus Petunia. Flowers of Petunia integrifolia emit mostly benzaldehyde, while flowers of Petunia axillaris subsp. axillaris emit a mixture of volatile benzenoid and phenylpropanoid compounds that include isoeugenol and eugenol. Flowers of the artificial hybrid Petunia hybrida, a cross between P. integrifolia and P. axillaris, emit a similar spectrum of volatiles as P. axillaris subsp. axillaris. However, the flowers of P. axillaris subsp. parodii emit neither isoeugenol nor eugenol but contain high levels of dihydroconiferyl acetate in the petals, the main scent-synthesizing and scent-emitting organs. We recently showed that both isoeugenol and eugenol in P. hybrida are biosynthesized from coniferyl acetate in reactions catalyzed by isoeugenol synthase (PhIGS1) and eugenol synthase (PhEGS1), respectively, via a quinone methide-like intermediate. Here we show that P. axillaris subsp. parodii has a functional EGS gene that is expressed in flowers, but its IGS gene contains a frame-shift mutation that renders it inactive. Despite the presence of active EGS enzyme in P. axillaris subsp. parodii, in the absence of IGS activity the coniferyl acetate substrate is converted by an as yet unknown enzyme to dihydroconiferyl acetate. By contrast, suppressing the expression of PhIGS1 in P. hybrida by RNA interference also leads to a decrease in isoeugenol biosynthesis, but instead of the accumulation of dihydroconiferyl acetate, the flowers synthesize higher levels of eugenol.The Plant Journal 03/2009; 58(6):961-9. · 6.58 Impact Factor
Article: Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain.[Show abstract] [Hide abstract]
ABSTRACT: The phenylpropene t-anethole imparts the characteristic sweet aroma of anise (Pimpinella anisum, family Apiaceae) seeds and leaves. Here we report that the aerial parts of the anise plant accumulate t-anethole as the plant matures, with the highest levels of t-anethole found in fruits. Although the anise plant is covered with trichomes, t-anethole accumulates inside the leaves and not in the trichomes or the epidermal cell layer. We have obtained anise cDNA encoding t-anol/isoeugenol synthase 1 (AIS1), an NADPH-dependent enzyme that can biosynthesize t-anol and isoeugenol (the latter not found in anise) from coumaryl acetate and coniferyl acetate, respectively. In addition, we have obtained a cDNA encoding S-[methyl-14C]adenosyl-l-methionine:t-anol/isoeugenol O-methyltransferase 1 (AIMT1), an enzyme that can convert t-anol or isoeugenol to t-anethole or methylisoeugenol, respectively, via methylation of the para-OH group. The genes encoding AIS1 and AIMT1 were expressed throughout the plant and their transcript levels were highest in developing fruits. The AIS1 protein is 59% identical to petunia (Petunia hybrida) isoeugenol synthase 1 and displays apparent Km values of 145 microm for coumaryl acetate and 230 microm for coniferyl acetate. AIMT1 prefers isoeugenol to t-anol by a factor of 2, with Km values of 19.3 microm for isoeugenol and 54.5 microm for S-[methyl-14C]adenosyl-l-methionine. The AIMT1 protein sequence is approximately 40% identical to basil (Ocimum basilicum) and Clarkia breweri phenylpropene O-methyltransferases, but unlike these enzymes, which do not show large discrimination between substrates with isomeric propenyl side chains, AIMT1 shows a 10-fold preference for t-anol over chavicol and for isoeugenol over eugenol.Plant physiology 12/2008; 149(1):384-94. · 6.56 Impact Factor
Article: The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages.[Show abstract] [Hide abstract]
ABSTRACT: Many plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Clarkia breweri flowers emit a mixture of eugenol and isoeugenol, while Petunia hybrida flowers emit mostly isoeugenol with small amounts of eugenol. We recently reported the identification of a petunia enzyme, isoeugenol synthase 1 (PhIGS1) that catalyzes the formation of isoeugenol, and an Ocimum basilicum (basil) enzyme, eugenol synthase 1 (ObEGS1), that produces eugenol. ObEGS1 and PhIGS1 both utilize coniferyl acetate, are 52% sequence identical, and belong to a family of NADPH-dependent reductases involved in secondary metabolism. Here we show that C. breweri flowers have two closely related proteins (96% identity), CbIGS1 and CbEGS1, that are similar to ObEGS1 (58% and 59% identity, respectively) and catalyze the formation of isoeugenol and eugenol, respectively. In vitro mutagenesis experiments demonstrate that substitution of only a single residue can substantially affect the product specificity of these enzymes. A third C. breweri enzyme identified, CbEGS2, also catalyzes the formation of eugenol from coniferyl acetate and is only 46% identical to CbIGS1 and CbEGS1 but more similar (>70%) to other types of reductases. We also found that petunia flowers contain an enzyme, PhEGS1, that is highly similar to CbEGS2 (82% identity) and that converts coniferyl acetate to eugenol. Our results indicate that plant enzymes with EGS and IGS activities have arisen multiple times and in different protein lineages.The Plant Journal 06/2008; 54(3):362-74. · 6.58 Impact Factor
Article: Cloning of lipoxygenase genes from a cyanobacterium, Nostoc punctiforme, and its expression in Eschelichia coli.[Show abstract] [Hide abstract]
ABSTRACT: Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information about LOXs in cyanobacteria has been reported. Herein, we report the first isolation of two LOX genes (NpLOX1 and NpLOX2) from a cyanobacterium, Nostoc punctiforme ATCC29133. Incubations of recombinant NpLOX1 and NpLOX2 proteins expressed in Eschelichia coli with linoleic acid resulted in the predominant formation of linoleic acid 13-S-hydroperoxide. Other C18 and C20 fatty acids could also be substrates for NpLOX enzymes. Phylogenetic analysis of NpLOX sequences showed that the NpLOX enzymes shared a high homology with LOX sequence of a bacterial pathogen, Pseudomonas aeruginosa, and these bacterial LOXs formed a subfamily distinct from those of plants, algae, and mammals.Current Microbiology 05/2007; 54(4):315-9. · 1.52 Impact Factor
Article: Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol.[Show abstract] [Hide abstract]
ABSTRACT: Petunia flower petals emit large amounts of isoeugenol, which has been shown to be synthesized by isoeugenol synthase (PhIGS1) from an ester of coniferyl alcohol, hypothesized to be coniferyl acetate. This paper describes the identification and characterization of a novel petunia gene encoding an enzyme belonging to the BAHD acyltransferase family whose expression correlates with isoeugenol biosynthesis. RNAi suppression of this gene results in inhibition of isoeugenol biosynthesis. Biochemical characterization of the protein encoded by this gene showed that it has acetyltransferase activity and is most efficient with coniferyl alcohol among the alcohol substrates tested. Overall, these data support the conclusion that coniferyl acetate is the substrate of isoeugenol synthase.The Plant Journal 02/2007; 49(2):265-75. · 6.58 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Phenylpropenes, a large group of plant volatile compounds that serve in multiple roles in defense and pollinator attraction, contain a propenyl side chain. Eugenol synthase (EGS) catalyzes the reductive displacement of acetate from the propenyl side chain of the substrate coniferyl acetate to produce the allyl-phenylpropene eugenol. We report here the structure determination of EGS from basil (Ocimum basilicum) by protein x-ray crystallography. EGS is structurally related to the short-chain dehydrogenase/reductases (SDRs), and in particular, enzymes in the isoflavone-reductase-like subfamily. The structure of a ternary complex of EGS bound to the cofactor NADP(H) and a mixed competitive inhibitor EMDF ((7S,8S)-ethyl (7,8-methylene)-dihydroferulate) provides a detailed view of the binding interactions within the EGS active site and a starting point for mutagenic examination of the unusual reductive mechanism of EGS. The key interactions between EMDF and the EGS-holoenzyme include stacking of the phenyl ring of EMDF against the cofactor's nicotinamide ring and a water-mediated hydrogen-bonding interaction between the EMDF 4-hydroxy group and the side-chain amino moiety of a conserved lysine residue, Lys132. The C4 carbon of nicotinamide resides immediately adjacent to the site of hydride addition, the C7 carbon of cinnamyl acetate substrates. The inhibitor-bound EGS structure suggests a two-step reaction mechanism involving the formation of a quinone-methide prior to reduction. The formation of this intermediate is promoted by a hydrogen-bonding network that favors deprotonation of the substrate's 4-hydroxyl group and disfavors binding of the acetate moiety, akin to a push-pull catalytic mechanism. Notably, the catalytic involvement in EGS of the conserved Lys132 in preparing the phenolic substrate for quinone methide formation through the proton-relay network appears to be an adaptation of the analogous role in hydrogen bonding played by the equivalent lysine residue in other enzymes of the SDR family.PLoS ONE 02/2007; 2(10):e993. · 3.53 Impact Factor
Article: Chavicol formation in sweet basil (Ocimum basilicum): cleavage of an esterified C9 hydroxyl group with NAD(P)H-dependent reduction.[Show abstract] [Hide abstract]
ABSTRACT: Propenyl- and allyl-phenols, such as methylchavicol, p-anol and eugenol, have gained importance as flavoring agents and also as putative precursors in the biosynthesis of 9,9'-deoxygenated lignans, many of which have potential medicinal applications. In spite of several decades of investigation, however, the complete biosynthetic pathway to a propenyl/allylphenol had not yet been reported. We have subjected a Thai basil variety accumulating relatively large amounts of the simplest volatile allylphenol, methylchavicol, to in vivo administration of radiolabeled precursors and assays of protein preparations in vitro. Through these experiments, the biosynthesis of chavicol was shown to occur via the phenylpropanoid pathway to p-coumaryl alcohol. Various possibilities leading to deoxygenation of the latter were examined, including reduction of the side-chain double bond to form p-dihydrocoumaryl alcohol, followed by dehydration to afford chavicol, as well as formation of p-methoxycinnamyl alcohol, with further side-chain modification to afford methylchavicol. A third possibility studied was activation of the side-chain alcohol of p-coumaryl alcohol, e.g.via esterification, to form a more facile leaving group via reductive elimination. The latter was shown to be the case using p-coumaryl esters as potential substrates for a NAD(P)H-dependent reductase to afford chavicol, which is then O-methylated to afford methylchavicol.Organic & Biomolecular Chemistry 08/2006; 4(14):2733-44. · 3.57 Impact Factor
Article: Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester.[Show abstract] [Hide abstract]
ABSTRACT: Phenylpropenes such as chavicol, t-anol, eugenol, and isoeugenol are produced by plants as defense compounds against animals and microorganisms and as floral attractants of pollinators. Moreover, humans have used phenylpropenes since antiquity for food preservation and flavoring and as medicinal agents. Previous research suggested that the phenylpropenes are synthesized in plants from substituted phenylpropenols, although the identity of the enzymes and the nature of the reaction mechanism involved in this transformation have remained obscure. We show here that glandular trichomes of sweet basil (Ocimum basilicum), which synthesize and accumulate phenylpropenes, possess an enzyme that can use coniferyl acetate and NADPH to form eugenol. Petunia (Petunia hybrida cv. Mitchell) flowers, which emit large amounts of isoeugenol, possess an enzyme homologous to the basil eugenol-forming enzyme that also uses coniferyl acetate and NADPH as substrates but catalyzes the formation of isoeugenol. The basil and petunia phenylpropene-forming enzymes belong to a structural family of NADPH-dependent reductases that also includes pinoresinol-lariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase.Proceedings of the National Academy of Sciences 07/2006; 103(26):10128-33. · 9.81 Impact Factor
Article: Rice fatty acid alpha-dioxygenase is induced by pathogen attack and heavy metal stress: activation through jasmonate signaling.[Show abstract] [Hide abstract]
ABSTRACT: Plant fatty acid alpha-dioxygenases (DOXs) catalyze the stereospecific conversion of fatty acids into the corresponding (R)-2-hydroperoxy fatty acids. In several plant species the corresponding gene was shown to be induced by pathogen infection, herbivore attack and environmental stresses. The precise signaling pathway accountable for the induction remains unidentified. In the present study, the effects of bacterial infection, oxidative- and heavy metal-stresses, and plant signaling molecules such as jasmonate, salicylic acid (SA), and ethylene (ET) on expression of a fatty acid alpha-DOX (OsDOX) gene in rice seedlings were investigated. The rice blight bacteria, Xanthomonas oryzae, elicited the accumulation of OsDOX transcripts in the leaves in both the incompatible and compatible interactions. Treating the seedling with CuSO4 also significantly enhanced the OsDOX expression. The degree of induction was shown to be mostly parallel to the level of endogenous jasmonic acid (JA) in the leaves. In contrast, SA was little effective and ET down-regulated not only the OsDOX expression but also the endogenous level of JA in rice seedlings. These results suggested that the OsDOX gene expression by a variety of stress-related stimuli was activated through jasmonate signaling and was negatively regulated by ET.Journal of Plant Physiology 09/2005; 162(8):912-20. · 2.77 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The cDNA from barley coding FA hydroperoxide lyase (HPL) was cloned. A recombinant protein derived from the cDNA was expressed in Escherichia coli as an active enzyme. Thus far, there have been no reports on HPL in monocotyledonous plants. The recombinant protein was shown to be most active to linolenic acid 13-hydroperoxide, followed by linoleic acid 13-hydroperoxide. 9-Hydroperoxides of the FA could not be substrates for the recombinant HPL. The activity was dramatically enhanced in the presence of a detergent and/or a salt in the reaction mixture. At the same time, the kinetics of the reaction, including inactivation and the Vmax value of the HPL, were also greatly modulated, depending on the concentration of a monovalent cation and/or a detergent in the reaction mixture. These results suggest that these effectors induced a conformational change in barley HPL, resulting in an improvement in substrate binding and in enzyme activity.Lipids 12/2003; 38(11):1167-72. · 2.56 Impact Factor
Article: Catalytic properties of rice alpha-oxygenase. A comparison with mammalian prostaglandin H synthases.[Show abstract] [Hide abstract]
ABSTRACT: Long-chain fatty acids can be metabolized to C(n)(-1) aldehydes by alpha-oxidation in plants. The reaction mechanism of the enzyme has not been elucidated. In this study, a complete nucleotide sequence of fatty acid alpha-oxygenase gene in rice plants (Oryza sativa) was isolated. The deduced amino acid sequence showed some similarity with those of mammalian prostaglandin H synthases (PGHSs). The gene was expressed in Escherichia coli and purified to apparently homogeneous state. It showed the highest activity with linoleic acid and predominantly formed 2-hydroperoxide of the fatty acid (C(n)), which is then spontaneously decarboxylated to form corresponding C(n)(-1) aldehyde. With linoleic or linoleic acids as a substrate, rice alpha-oxygenase formed no product having a lambda(max) at approximately 234 nm, which indicated that the enzyme could not oxygenize the pentadiene system in the substrate. The spectroscopic feature of the purified enzyme in its ferrous state is similar to that of mammalian PGHS, whereas that of dithionite-reduced state showed significant difference. Site-directed mutagenesis revealed that His-158, Tyr-380, and Ser-558 were essential for the alpha-oxygenase activity. These residues are conserved in PGHS and known as a heme ligand, a source of a radical species to initiate oxygenation reaction and a residue involved in substrate binding, respectively. This finding suggested that the initial step of the oxygenation reaction in alpha-oxygenase has a high similarity with that of PGHS. The rice alpha-oxygenase activity was inhibited by imidazole but hardly inhibited by nonsteroidal anti-inflammatory drugs, such as aspirin, ibuprofen, and flurbiprofen, which are known as typical PGHS inhibitors. In addition, peroxidase activity could not be detected with alpha-oxygenase when palmitic acid 2-hydroperoxide was used as a substrate. From these findings, the catalytic resemblance between alpha-oxygenase and PGHS seems to be evident, although there still are differences in their substrate recognitions and peroxidation activities.Journal of Biological Chemistry 07/2002; 277(25):22648-55. · 4.65 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Long-chain fatty acids can be metabolized to Cn −1 aldehydes by α-oxidation in plants. The reaction mechanism of the enzyme has not been elucidated. In this study, a complete nucleotide sequence of fatty acid α-oxygenase gene in rice plants (Oryza sativa) was isolated. The deduced amino acid sequence showed some similarity with those of mammalian prostaglandin H synthases (PGHSs). The gene was expressed in Escherichia coli and purified to apparently homogenous state. It showed the highest activity with linoleic acid and predominantly formed 2-hydroperoxide of the fatty acid (Cn), which is then spontaneously decarboxylated to form corresponding Cn −1 aldehyde. With linoleic or linoleic acids as a substrate, rice α-oxygenase formed no product having aλ max at approximately 234 nm, which indicated that the enzyme could not oxygenize the pentadiene system in the substrate. The spectroscopic feature of the purified enzyme in its ferrous state is similar to that of mammalian PGHS, whereas that of dithionite-reduced state showed significant difference. Site-directed mutagenesis revealed that His-158, Tyr-380, and Ser-558 were essential for the α-oxygenase activity. These residues are conserved in PGHS and known as a heme ligand, a source of a radical species to initiate oxygenation reaction and a residue involved in substrate binding, respectively. This finding suggested that the initial step of the oxygenation reaction in α-oxygenase has a high similarity with that of PGHS. The rice α-oxygenase activity was inhibited by imidazole but hardly inhibited by nonsteroidal anti-inflammatory drugs, such as aspirin, ibuprofen, and flurbiprofen, which are known as typical PGHS inhibitors. In addition, peroxidase activity could not be detected with α-oxygenase when palmitic acid 2-hydroperoxide was used as a substrate. From these findings, the catalytic resemblance between α-oxygenase and PGHS seems to be evident, although there still are differences in their substrate recognitions and peroxidation activities.Journal of Biological Chemistry 06/2002; 277(25):22648-22655. · 4.65 Impact Factor
Top Journals
Kyoto University
Kioto, Kyōto, Japan
- Research Institute for Sustainable Humanosphere
University of Michigan
Ann Arbor, MI, United States
- Department of Molecular, Cellular and Developmental Biology
Yamaguchi University
Yamaguchi-shi, Yamaguchi-ken, Japan
- Department of Biological Chemistry


text:
Latest Videos
Whistler Blackcomb November 10, 2014 - Wake Up, Winter has Arrived at Whistler Blackcomb!
Do you ever have those dreams where you're shredding the peaks of epic coastal mountains on a bluebird day with your buds, avy dogs are playing in the snow and new gondola cabins are glistening in the sun? IT'S NOT A DREAM! Well, the dream came alive for Stan Rey and Mercedes Nicoll yesterday, November 10, with a little help from our friends at Blackcomb Aviation.
With Optik TV™ you can remote record your favourite shows from a smartphone or computer anywhere you get an Internet connection, even the mountain.

subset: commoncrawl


text:
by Zolton in Video on Thursday 6 August 2009
This beautifully animated clip for Kate Miller-Heidke's new song The Last Day On Earth was created by Sydney artist Jefferton James.
Share your favourite cultural finds with the Lost At E Minor community and become famous in the process. Well, kinda.
by Milo Sumner
by Inigo del Castillo
by Nicole Hughes
by Lost At E Minor
by Kenny Ong
© Lost at E Minor | Image Attribution | Privacy Policy
Don't show this again - I already like Lost At E Minor


text:
G5 T3 Broadhead Replacement Blades Kit
Product #: 185069 G5 Outdoors #: 178 UPC #: 817990001784
Product Information
Having a sharp broadhead is essential to successful bowhunting. Ensure that your T3 Broadheads will perform at their best with the Replacement Blade Pack from G5. This pack includes 9 blades, 6 washers, and 3 spider clips.
Delivery Information
The total weight of the product, including the product's packaging as it sits on our shelf. Since some products ship directly from the Manufacturer, their weight may be unavailable. Because of variations in the manufacturing process, similar products may have differing weights. Unless the product is designated as "Ships Alone", Shipping Weight does not include the shipping box or packing materials associated with your order.
Product ships from MidwayUSA, separate from any other products on your order due to its size. Products that are Shipped Alone incur separate "Per Order" and "Per Pound" shipping charges.
Product is regulated by the US Department of Transportation (DOT) and cannot be shipped via USPS or air shipping methods. DOT-Regulated products must ship UPS Ground.
Product is restricted by shipping carriers and cannot be shipped to certain locations or via certain shipping methods.
Additional shipping charge for HazMat products. Carriers charge extra to ship packages with products they consider hazardous, such as reloading powder and primers.
Was this information helpful? Yes No
Thank you! Your valuable input helps improve our product information and future product selections
Know someone who may like this? Email this Page to a Friend
Customer Reviews
The statements and opinions expressed within Customer Reviews reflect each author's personal perspective and do not imply endorsement by MidwayUSA, its Employees or any other organization. Customers should in no way infer that MidwayUSA has validated review information as safe, accurate or appropriate.
NOTE: Prices, specifications and availability are subject to change without notice.
We reserve the right to correct typographic, photographic and/or descriptive errors.
We reserve the right to correct typographic, photographic and/or descriptive errors.
Suggested Products
Others Bought
- G5 T3 Mechanical Broadhead 100 Grain Stainless Steel Pack of 3...
Hoppe's Target Sighting-In 14" x 14" Package of 100...
Barnes VOR-TX Ammunition 270 Winchester 130 Grain Tipped...
Energizer Battery CR2032 Lithium Pack of 2...
Barnes VOR-TX Ammunition 30-06 Springfield 180 Grain Tipped...
$31.37Clearance $42.99 Save $11.62 (27%)
- Outdoor Edge Swingblade Hunting Knife AUS-8 Stainless Steel...
Rainier LeadSafe Bullets 9mm (355 Diameter) 115 Grain Plated...
$15.29 - $74.99
Rainier LeadSafe Bullets 40 S&W, 10mm Auto (400 Diameter) 180...
$19.29 - $127.99
CVA 4-in-1 T-Loader (Preloader, Powder Measure, Bullet...
Stealth Cam SD Memory Card...
$7.19 - $13.49
Carbon Express Launch Pad Lighted Arrow Nock...
Nosler Ballistic Tip Hunting Bullets 30 Caliber (308 Diameter)...


text:
TOYOTA Avensis-Estate Lease CLONTARF
Use our system to find the right TOYOTA Avensis-Estate CLONTARF Car lease for you. We have many leasing companies on our system all wanting to quote cheap prices. Fill in the form to find cheaper TOYOTA Avensis-Estate CLONTARF Car lease.
Sample Of Recent Leasing Requests:
|25th Nov 2014 11.16pm
|BMW 3 Series Saloon
|25th Nov 2014 10.14pm
|VOLKSWAGEN Passat Saloon Blue Motion
|25th Nov 2014 08.23pm
|KIA Sportage
|25th Nov 2014 04.13pm
|BMW 5 Series Saloon
|25th Nov 2014 10.06am
|CITROEN C Crosser Station Wagon
|25th Nov 2014 04.24am
|AUDI A5 Coupe
|24th Nov 2014 11.10pm
|SKODA Octavia Hatchback
|24th Nov 2014 11.10pm
|SKODA Octavia Hatchback
|24th Nov 2014 09.52pm
|TOYOTA Hilux
|24th Nov 2014 08.18pm
|KIA Sportage
Local TOYOTA Avensis-Estate Lease Suppliers In CLONTARF Area
Some might like to business local in the CLONTARF area. We will attept to get you a TOYOTA Avensis-Estate CLONTARF quote in the CLONTARF area from local dealers. Some national companies still have regional offices to distribute their TOYOTA Avensis-Estate Car lease vehicles
UK "Toyota Avensis Estate" Options For Your Next Lease Option in CLONTARF
It's the fool-proof process that makes LeaseBamIreland so popular. Finding the "Toyota Avensis Estate" spare you need for the CLONTARF area could not be any easier. We also have the facility for you to place your vehicle lease request for the CLONTARF area via telephone. Just call ONLINE ONLY to speak to one of our personnel. Our committment to help you find Car lease deals in CLONTARF makes us your one-stop-shop for the cheapest deal.
Business "Toyota Avensis Estate" Lease Quotes for CLONTARF
We have access to a wide selection of suppliers in CLONTARF who will quote you for your next "Toyota Avensis Estate" lease options:
- "Toyota Avensis Estate" New And Used Vehicles
- "Toyota Avensis Estate" Benefit From What Excess Vehicles There Are
- "Toyota Avensis Estate" Business Vehicles within CLONTARF
- "Toyota Avensis Estate" Including Maintenance in CLONTARF
Save Time And Money On "Toyota Avensis Estate" Leasing within CLONTARF
LeaseBamIreland can save you time; by finding vehicles from a great number of UK showrooms and specialists to provide you with the cheapest options available in CLONTARF. LeaseBamIreland puts you in touch with what is on offer around CLONTARF. We only work with reputable "Toyota Avensis Estate" lease companies to take part in our service, offering you fast delivery on all new and pre-owned Car lease offers in CLONTARF.


text:
- Summary: Check it out... We're ToeJam and Earl. And we ran into a small problem. More specifically, a large planet (I shoulda never let Earl drive...). Help us hunt down the pieces to our rocket ship and we'll let you jam out some tunes on our megawatt rapmaster. Deal? WHOAH!! We're bumpin' into some of the weirdest creatures in the universe: Earthlings. Boogie down with bewitching hula girls. Block a nerd herd. Or hurl tomatoes at a crazed dentist with a drill. Split up and scope things out. Or hang together to share your stuff. Pick up presents you can use - like wings, rocket skates and inner tubes. Or ones you'll wish you hadn't opened - like bees, tomato rain and school books. So grab a friend - or join the jamminest party you'll ever play!… Expand
- Developer: M2
- Genre(s): General
- Cheats: On GameFAQs
- More Details and Credits »
Score distribution:
Positive: 0 out of
Mixed: 0 out of
Negative: 0 out of
Score distribution:
Positive: 2 out of 2
Mixed: 0 out of 2
Negative: 0 out of 2
Nov 9, 2012$5 for one of the greatest games of all time? Join me in feeling like a thief for having to pay so little to gain so much. When was the last time you put on a little Toejam and Earl, used icarus wings to fly over to level 0 and chill in the hot tub? Don't think that this game has aged poorly because it hasn't. The music is still top-notch and the rougelike-with-presents gameplay is still as fun as it ever was.… Expand
Feb 3, 2013Considering this is a game that came from the greatest decade of gaming, the 90's, and not to mention, was, and still is, one of my top ten video games of all time, it's hard to really knock anything about it. The only reason it got a 9/10 was because, though I loved the news that this game would have online, co-op gameplay, it's a tad rough finding someone to play with, and even then, I've had numerous games drop or stall due to internet connections...although that's not their fault. The game was never perfect, but it never fails to entertain. For five bucks, you're doing yourself a disjustice by not buying it. I own the original, but felt obligated to fork over five more dollars, if for no other reason than to show everyone involved that it was a brilliant idea to include this on the PSN with trophies and online capabilities. The next five bucks that falls into your pocket should go straight to picking this gem up, if you haven't played it...unless you didn't like it twenty years ago. This game's a steal!… Expand


text:
Welcome to Saint Joseph Academy
Mission Statement
The administrators, faculty, and staff of Saint Joseph Academy minister to the spiritual and academic needs of its students and provide a supportive environment where students become more proactive in their Catholic faith, successful, confident, and self motivated learners in a complex technological society, and active contributors to the world community.
Announcements, News and Upcoming Events
CLASSROOM SPOT LIGHT 8th grade
The Class of 2015 is off to an exciting & busy year! The 8th graders are preparing to take the COOP...
2014 1st Grade Class
Mrs. Capizzi's 1st Grade Class I am so thankful to be a first grade teacher at St. Joseph Acad...
Barnes & Noble Book Fair
Barnes & Noble Bookfair Saturday December 6th, 2014 9 a.m. to 11 p.m....
The Green Knight Gazette
Hello St. Joseph Academy! Thanks you for reading our first issue of the Green Knight Gazette. We ho...
SJACT SJACT is our brand new enrichment program in Acting and Theater Arts. Our small group of...
Student Council 2014
Student Council Mrs. Marie Sniffen The Saint Joseph Academy (SJA) Student Council held its induct...
November News in Spanish
Five members of the class of 2015 produced SJA's first Spanish newscast...
read more >>
Upcoming events
CLASSROOM SPOT LIGHT 8th grade
November News in Spanish
Barnes & Noble Book Fair
Student Council 2014
2014 1st Grade Class
The Green Knight Gazette
Home School Association
The Home School Association membership is comprised of parents/guardians and staff of Saint Joseph Academy.
Calling all Alumni
We invite the alumni to register here and Spread the news, come and visit. We look forward to seeing you
Maschio's Calendar
Bananas are a good source of fiber, potassium and vitamin C.
Did you Know?
You can store a ripe banana in the refrigerator, the peel will turn brown but the fruit inside will be good and deli-cious!


text:
Inspirational Quotes, Motivational Sayings - Page 4
The best feeling in life is doing something that others said you could not do.
There will be a moment in your life where you'll just want to give up but you must push yourself to continue its those moments that make us stronger and make that next moment just a little easier. Never give up on yourself and others will never give up on you.
Your life is full of opportunities and possibilities. It is yours to create, to enjoy or to suffer. You may lead a successful or failing life – depending on how much you are committed to and prepared for your life.
– Dr T.P.Chia
Higher the mountain, greater the obstacle, sweeter the victory.
When life puts you in tough situations, don't say why me? Just say try me!
Live every day like it's your last.
You cannot direct the wind but you can adjust the sails.
A journey of thousand miles must begin with a single step.
– Lao Tzu
The quality of a person's life is in direct proportion to their commitment to excellence, regardless of their chosen field of endeavor.
– Vince Lombardi
A year from now you may wish you had started today.
– Karen Lamb
When you think you are down – smile- beacause the only way left is UP!
First, know where you want to go. Then, go where you want to go. You are your own pilot and you can choose your destination.
Never give in, never, never, never, in nothing great or small, large or petty, never give in except to convictions of honor and good sense. Never yield to a force; never yield to the apparently overwhelming might of the enemy.
– Winston Churchill
Hardships often prepare ordinary people for an extraordinary destiny.
– C. S. Lewis
The expert in anything was once a beginner.
Adopt the pace of nature: her secret is patience.
– Ralph Waldo Emerson
The pain you feel today is the strength you feel tomorrow. For every challenge encountered there is opportunity for growth.
You can have anything you want if you are willing to give up the belief that you can't have it.
Do something good today and in the future you will get repaid with something good too. (Do something good. Get something good.)
To get anywhere you must first start somewhere.
Stand up for what you believe in, even if you're standing alone.
There are no failures, only feedback…!!
Never say "I can't" because you can and you will it takes practice to make perfect.
People who push the limits sometimes learn the limits might push back.
Worries End where Faith Begins……


text:
Los Alcazares Villa Rental Photos and Description
Self catering rental villa with pool close to the beach,restaurants,bars,shops
Beautiful private detached rental villa in Los Alcazares close to the beach, town and the golf courses of Roda golf resort and La Serena. The villa is excellently decorated with everything required for holiday rental, the villa is furnished to a high standard with satellite TV. The summer rental villa has three bedrooms, one double and two twins. A large patio with drive way at the front of the property and a private roof top solarium on the roof. The holiday villa has a kidney shaped private swimming pool set in large private gardens. The separate kitchen has everything required for self catering holidays leading to the dining area and the large patio for outdoor dining. The lounge has TV, DVD, CD and radio. The beach, restaurants, shops and bars are all within 5 minutes walking distance and the golf courses are a few minutes by car. The rental villa is an excellent self catering home for all year round holidays with easy access from the local airport of Murcia.
- Suitability:
- children welcome
- pets considered
- wheelchair accessible


text:
Buy online front row tickets for all Concert, Theater and Sporting events, and tickets to all sold out major events.
Sport, Concert and Theater tickets are hot and Frontrow.com has tickets to all your favorite events, including the Miami Heat Games, College Football and Super Bowl. We can get you "In the door" or the best seats to all games and events. Talk to one of our specialists or order online 24/7. Frontrow.com has a solid reputation of 22 years in the business selling tickets. Front row can get you tickets for all the, Playoffs and all NBA Games.
The Arabian Nights is always a great hit with every crowd. We stock all The Arabian Nights tickets to every The Arabian Nights show! Frontrow.com is your souce for premium The Arabian Nights tickets. Let us put you right up front and see The Arabian Nights like you've never seen before, up close and personal. Order online or call toll free at 800-881-7691 and speak to a frontrow concert experts.
BUY - SELL The Arabian Nights Tickets
Sell Your The Arabian Nights Tickets
Frontrow.com provides the ervice of finding and obtaining tickets to events worldwide. We are a private company not affiliated with any venue, stadium, promoter, theater or box office, the MLB, NBA, NFL, NCAA, NHL,NBA or any of their teams. Trademark, trade names, and copyrights, used in this web site are only for descriptive purposes. We Do not act with the permission or on behalf of any of above mentioned organizations.. We provide The Arabian Nights tickets through a range of sources and contacts. Prices for The Arabian Nights tickets are often higher than the ticket's face value, reflecting costs of finding and aquiring premium The Arabian Nights Tickets.
About us
Front Row is a quality ticket broker for all concerts, theater, and sporting events. Front Row sells the finest The Arabian Nights packages and tickets. If tickets you are searching for are not available, please check back as we always update and list new tickets in our huge inventory. The Arabian Nights schedules are on our web site. Just click to see our real-time online concert, sport and theater tickets.
Frontrowcom has the largest inventory of The Arabian Nights Tickets on the secondary ticket market. Call today for up to the latest information on ticket availability and prices since we constantly purchase new event tickets. If you want to go to a special The Arabian Nights event; we are your ticket broker for sold out Tickets. Front Row welcomes corporate accounts seeking premium tickets for entertaining clients.
Front Row has been a ticket broker for over two decades. Our mission statement is to give our clients the best service, lowest prices and reliability. When searching for The Arabian Nights Tickets or other events, Call Frontrow.com where our professional team can offer you the ultimate The Arabian Nights Tickets experience.
Front Row.com is for all of your other ticketing needs. Frontrow.com sells tickets to all concert tours nationwide. Not only do we sell The Arabian Nights tickets but we also buy The Arabian Nights tickets, NFL tickets, NCAA tickets, NHL tickets, NBA, MLB, all golf tickets, tennis, title fight tickets, concerts, family show tickets, and theater tickets including Broadway and Las Vegas shows. Call Frontrow.com today for The Arabian Nights Tickets ; and all of your entertainment needs! Frontrow.com strives to provide our patrons the utmost quality service and competitive pricing. If any event isn't listed; please phone us for any hard to find or special requests.
PURCHASING AND AVAILABILITY OF The Arabian Nights Tickets
Click the above link for The Arabian Nights info and front row tickets to events across the country and globe. You can buy tickets for The Arabian Nights on our web site by selecting your specific venue and time. When searching for The Arabian Nights tickets that are unavailable or sold-out from other sources, contact Frontrowcom, your source of front row tickets. If you don't see tickets on our frontrow web site or don't want to order online, don't hesitate to call us toll free at 800-881-ROW-1. We are ticket pro's and offer data about buying tickets, schedules and venue maps. Frontrow.com offers an vast inventory of theater, concert, sport event tickets with constant updates. At Frontrow.com; our goal is offering the largest selection of The Arabian Nights tickets on the secondary market. Use "Click Here" links to check future events, schedules and seating charts. Our The Arabian Nights inventory has up to the hour accuracy. However, if tickets you want are not available, please contact us and our staff will guide you through available options in the same or similar category to your original ticket choice.
Ticket delivery
All tickets are sent via Federal Express. Once you locate The Arabian Nights tickets you want to buy, they can be purchased online via our secure encrypted server. If you prefer more personal service, call us today and speak to any of our ticket brokers. The Arabian Nights tickets are usually sent within 6-24 hours. Your shipping address must match the billing address of your credit card. If you wish to ship to an alternate address; we require ID. Please contact one of our ticket experts to provide you with excellent service.
Ticket refund policy
All The Arabian Nights sales and all other event tickets are final.
The Daily Star
Musical tribute to the resilience of women
The Daily Star
For years, wide-eyed children have listened to the enchanting tales of the Arabian Nights, also known as One Thousand and One Nights. Not everyone knows about its narrator, Persian queen Scheherazade, who cured her murderous husband King ...
Sarnia Observer
Lambton Young Theatre Players presents an original play
Sarnia Observer
The Lambton Young Theatre Players production of Arabian Nights takes the stage at the Sarnia Library Theatre Dec. 2 through Dec. 6. The cast includes 26 young actors and actresses from across Lambton County. The play was written by Nancy Keys, artistic ...
DIFF reveals second Arabian Nights line-up
The second line-up of films in the Dubai International Film Festival's (DIFF) Arabian Nights programme provides a multi-dimensional view of the region and the hopes and struggles of its people. The films in this category acquaint audiences with the Middle ...
Calgary Herald
Charity event a feast of wine and costume revelry
Calgary Herald
From left, at the 21st Willow Park Wines & Spirits Charity Wine Auction are event co-chairs Scott Henuset and his wife Suzanne with Liz Henuset and her husband, Willow Park Wines owner Wayne Henuset. The Arabian Nights-themed event raised $323,000 ...
'Tales of the Marvellous and News of the Strange', introduced by Robert Irwin
Financial Times
Tales of the Marvellous, which has now been translated by Malcolm C Lyons, is understood to be the oldest Arab story collection ever discovered. Unlike the stories in the Arabian Nights, which were framed within the tale of Sheherazade, forced to rouse her ...
Check out Frontrow.com for all your tickets needs. We've got great sources for any event ranging from concert tickets to World Series tickets, Book of Mormon tickets, and Las Vegas show tickets. And don't forget the chance to score big on Broadway tickets and once in a lifetime events like the National Final Rodeo, the 2015 Super Bowl or the Daytona 500. Our inventory covers all types of entertainment and special events. With the biggest selection of concerts, sports, and theater tickets around, you can't go wrong with tickets from Frontrow.com.


text:
Thanks for the source quote. Yes, do send me particulars on the book. No, Hannah is not buried in MA, but in Illinois - quite a journey through her life she made. Scott email@example.com
|Home | Help | About Us | Site Index | Jobs | PRIVACY | Affiliate
|© 2007 The Generations Network


text:
- Music
- Dining
- Attractions
- Sports
- Theater
- Art
- Family Fun
- Fitness/Health
- Neighborhoods
- About
- The Weekender
Do you have diabetes, arthritis, high blood pressure, asthma, pain, depression, or other ongoing health problems? Join this 6-week workshop and learn to manage your chronic health condition and earn a book, relaxation CD, and 8-week YMCA membership! Free!
|Louisville Community Health Data Site Wants Your Contributions
|5 Places To Do Hot Yoga In Louisville
|Tart Farewell: Kentucky's Only CeFiore in the Highlands Closing
|Top 5 Ideas for Family Play this Week
|In Doga Class, Dogs, Owners Connect on the Mat
|13 Places to Take a Yoga Class for Five Bucks
|Six More Places to Take a Yoga Class for $5 in Louisville


text:
Senior Member
Silver smiled slightly at Lily's remarks.
"Well I couulld tell you... Though I don't think you would like his answer anyways.... And of course, Miss Lily, I'll do whatever you want... Its your heart anyways."
Ethelyn let out a startled squeak when the armor spoke, almost jumping out of her seat when she heard it. She clasped her hands together rather nervously, timidly replying to him as she did.
"... P-please... D-d-do..."
Bright Oblivion
Grand Viper
Lily laughed her usual laugh. "Can you make me fall in love with a six foot seven Freljordian with washboard abs? Now seriously, I want to be given the choice when I die. I'll explain more when Vince decides to come and talk."
Youay's suit spoke up yet again, There is a 47.421% stress increase in your back. I will repair this.
The doctor's back would start feeling a soothing massage drum away at his muscles.
When Rose yelled, it was not a normal yell. The miasma ignited and started to twist and burn around her in an amalgamation of holy flames and eldritch fire, burning the wall behind her. Her voice itself became convoluted, barely recognizable, "THEN WHAT DO YOU SEE?! I HAVE NOT SEEN MYSELF SINCE I WAS TEN! NOT SINCE I KILLED HIM! WHAT. DO. YOU. SEE?!" Rose's eyes flared up, her fists cricked as she tightened them, "Tell me what apparently everyone else sees, but I do not."
Lily clicked, "Sooo...anyone else hear that yelling?"
Silver couldn't help but laugh at Lily's first question.
"I think you should have made that choice a long time ago, Miss Lily. But anyways, we can talk about that later... Perhaps when everyone leaves..."
Her antennae twitched once as she heard Rose shouting. She sighed and rolled her eyes
"Miss Lily, you don't mind peeking outside for a moment, yeah?"
She then walked over to the door without waiting for the mechanical hand's answer, opened it and leaning against the door frame, but not stepping completely outside. She eyed the demon hunter for a moment before speaking rather bluntly to her.
"Seeing as you're asking this question to everyone in the entirety of Piltover, I'll gladly tell you what I see. I see a lost little girl crawling around in an adults body, screaming and crying 'woe is me, no one understands me!' yet she barely gives anyone a chance to try. You're miserable and wallowing around in your own little, disgusting mud pit of the past, yet ignoring the people who are trying to help you out of it. So you killed a few people? Big whoop, so have I. Mothers, fathers, children, you name it, I've probably torn their head off at one point or another. Do I enjoy that fact? No, but I deal with it and continue to function as a 'normal' being. And yes, I see that you have about a billion other problems, but guess what? Its time to be a big girl now, Rose, and face your problems because you're going absolutely nowhere by continuing to sob about them."
Ethelyn heard the voice from the suit, and pouted slightly as it spoke. That was her job to help Youay relax. She was about to say something else when Rose started her shouting, and she let out a startled squeak before latching back onto him again and burying her face on his shoulder.
As he heard the yelling outside, and felt Rose's aura intensify, he tensed. His hands closed into fists and his jaw set stubbornly as he fought against the overwhelming desire to go out and rip into Rose, possibly literally. As Silver spoke, he sat like a very annoyed statue, staring straight in front of him, barely registering the words she said to Rose. Emily probably wasn't going to like that.
Emily would have responded to Rose, and told her what she saw, were it not for Silver cutting in and pounding the beehive with a stick. Really? Did she actually think she was helping, or did she just think it was her divine mission to antagonize Rose and ruin anything anyone else was trying to do for her? Mentally, Emily railed, torn between wanting to scream and wanting to strangle Silver for making what she was attempting to do all that much more impossible. Physically, she took a deep breath through her nose before turning to Silver with a very forced calm and speaking in a tone that, despite the politeness of the words, held a sharp edge.
"Miss Silver, please go back inside and close the door."
Her eyes flicked to Lily, almost silently asking the hand for help in dealing with the alien. She had enough on her plate dealing with an irritable demon hunter without Silver's 'help'.
Lily waved at Silver on her way out the door, "No no, go ahead, this will be entertaining for the both of us."
Once Silver had finished saying her piece, the miasma grew thicker. Rose's eyes narrowed as she growled at the alien, "And how do you suggest I do that? Almost every single one of my problems was solved by killing it. There is something opposing you? Strike it down. There is someone in your way? Slaughter them. You have emotions? Dismiss them. You have a conscience? Remove it. That is what I was taught, that is what I became, that is what I have done for so many years, so how exactly do you suggest I 'suck it up.' You wouldn't even be hearing about my problems if it weren't for her!"
When Rose pointed at Lily, the flames dripped off her finger and hissed upon touching the floor, purple tendrils curling and disappearing the moment it touched stone.
"And why am I the one being blamed here?"
"And I put Vince in one almost every other week...day, whatever, point is so what?"
"The point is why are you keeping me here? Why do you want me to be here? You went out of your way to find me when I was leaving! I am not your problem!" She pointed at Silver, "Or hers!" She pointed at Emily, "Nor hers! Like Silver said, boo hoo, poor me, that is what I get for when I try speaking even the slightest bit about my d*mnable feelings! I am still getting used to talking! This is probably the most I've said in years! I don't remember saying this much in a week nevermind in the past hour with you people!" The demon hunter took a step towards Silver, her teeth bared, her blue eyes now completely lost as even they started to spark with fire. "You don't like me in the slightest, Alien. You have no reason to help me, none of you do, except for something that will benefit you! The hand wants me to stay here to act as a guardian to her child for some gods' forsaken reason! Why me over anyone else?! Why not you, alien, or Emily, or the werewolf, or literally anyone else more trustworthy than I?!"
Rose swatted at the air as she spoke in the an mocking tone. "So thank you for your generous hospitality, give me my whip and I will leave you and take my problems, a lost little girl crawling around in an adults body, screaming and crying 'woe is me, no one understands me, and I will gladly leave because it is not whether anyone understands me! I don't understand how they look at me and don't see what I see!"
Rose grabbed at her face and promptly tore off a healthy chunk of it, showing the muscle that lined her skull. Her free hand's fingernails dug into the muscle and parted it, until she finally showed her cheekbone. On the bloodied bone, one would see the source of the flames, flitting out in little spurts from strange, runic engravings on the actual bone itself. If one knew the glyphs well enough, they would be recognized as angelic glyphs.
"...So Silver, maybe you were a little too blunt there, huh? The girl's kinda nuts." Lily whispered in the alien's ear.
"I am a weapon, through and through! I was made to be a weapon, my entire body thrives on being a weapon, that is all I was taught to do, that is all I am capable of, that is my entire upbringing the moment I killed him. This is my problem, this is my issue, I will deal with it and I will leave you all be. I see no reason for any of you to help me." Rose slapped her flesh back clumsily into place, the miasma reached down and seemed to grab the bloodied mess. They carefully kneaded the skin and muscle back onto Rose's face, and thin scars the only evidence of her injury.
"That is what I see every, single time I look in the mirror. I don't see a person, I see a tool. I see a weapon. I see something that is broken, that doesn't work, that has a single duty and they are not fulfilling it. I see something that is not working. People want to offer me help when it is none of their business, it is not their place, they will gain nothing if they help me save for some selfish desire or want. I will deal with my problems, on my own, without any of you, so go ahead, d*mn me for my talking, insult me for speaking, you were all better off when I was a threat rather than an oddity to point at and wonder about because at least we kept distance from one another, give me my whip and I will leave you all be."
Lily seemed to almost laugh. She clicked quietly into Silver's ear, "Let's see if you and Emily here can do this diplomatically. I can't wait for the two of you to poke holes in her argument." The hand whirred, "On the other hand, Emily's starting to frighten me slightly. Maybe we should do what the nice lady says."
Silver looked as if she were about to say something to Rose, but remained silent as Emily spoke to her. She eyed her for a moment, her antennae twitching slightly. She then shrugged, simply saying one word in response.
She turned and slipped back inside, letting the door close behind her, muttering something in an alien language under her breath. The demon hunter had clearly irritated her in some way, though she wasn't going to be saying why any time soon.
Emily watched as Silver returned inside, visibly relaxing as the door closed. After a few moments, she turned to Rose.
"You say that you're a weapon, miss Rose, that your sole purpose is to kill others. You say that that's how you've been raised, that it's all you're capable of. And yet, the only person who has been physically harmed this morning is you. Despite being ravenously hungry, despite being absolutely furious, you've only spilled your own blood. Not mine. Not Sigmund's. Not miss Lily's. Not even miss Silver's, as infuriating as she can be sometimes. If killing is all you're capable of, shouldn't someone be dead by now, at your hands?"
Emily shook her head, regarding Rose calmly.
"But everyone's still alive, and all you've done is hurt yourself, something that no weapon can do. I can't dispute that killing is all you've been taught to do, but it's clearly not all you're capable of."
For a few moments, Emily went silent as she examined Rose, then nodded to herself.
"You want to know what I see? I see a woman who has enough strength to withstand the loss of her brother not once, but twice, both times at her hand. Killing is all you know. It's been all you've done for so long that you think it's all you're capable of. But something changed. All your emotions, all of the human things like hunger, pain, and exhaustion, everything that you've hurled into a box and buried away? It's all broken loose. And all you seem to want to do is lock it away and go back to the way things were. You don't like it. You aren't proud of it. But it's what you know, what you're comfortable with."
She smiled sadly and shook her head.
"I'm not sure how you could get back there, if you could get back there. And I don't think that your brother sacrificed himself just so everything could go back to how it was, in any case. Do you?"
She nodded.
"Yes. What you are has changed, and you're not able to do what you've been doing. But if there's little to no way to change what you are, then you have to change what you're doing. Right?"
Another pause.
"Miss Lily wants you to serve as the guardian of her child. Why you, and not me? Because I'm a healer, miss Rose. I'm not a protector. I deal with problems after they happen, but I'm not the best at stopping them. Why not 'the werewolf'? Because Sigmund is too busy trying to protect everyone else from himself. And because the demon hunters would enjoy nothing more than if they found him while he was trying to protect miss Nina. I'm sure you realize that."
She glanced toward the door and the alien behind it.
"Why you and not miss Silver? I don't know. Miss Silver doesn't know, and it probably irritates her. Miss Lily has her reasons. Good luck getting them from her before she wants to tell you, though."
Lucina moved out of the way, simply watching what he was going to do. Like she said, she wasn't here to stop him from doing anything. She remained there, quietly listening in. She wished her mother had done something like that with her incidence. But she couldn't help but feel he missed one of her points. When he mouthed to her she gave him a 'with any luck' smile. She moved to the wall, leaning up against it.
"I think I'm fine over here." She said with a friendly smile. She felt she should leave the room, but also felt that the wall was more comfortable than it should be.
Once Silver went back inside, Lily clicked, "Silver, a few things. Do humor me, please, because there is a point to this. Do you remember the last time our friend Jack was here? Do remind me, what was the last thing you did to him?"
Rose stared at Emily, not responding to a single one of her questions. The fire eventually died down to mere flickers, the miasma started to recede. The demon hunter looked away, leaned back on the wall and quietly thought. It was as though she was trying to process what was said, and how she would respond.
"...I don't...want to be a burden. To anyone else." Her blue eyes flicked over at Emily, finally making eye contact with the woman once more. "I'm not sure why I'm here, but if I stay, I will be a burden. Anyone who associates with me, ends up hurt. Dead. I draw in danger. Do you remember when we met at the restaurant? Your werewolf was nearly ready to attack me, not because of his own accord. I'm..." The demon hunter tilted her head, trying to find the word to best describe her. "I'm a beacon. It is hard not to notice my aura, my signal, anything. I'm...I'm honey, that attracts...flies?"
Rose shook her head, "That's stupid, that's not the saying. How did it go...?"
Vince patted Nina's head affectionately, "There we go, dear. Feeling a bit better?"
"Good. Now I'll be in the kitchen. Lucina, stay here with Nina for as long as you want, if anything is up, come get me. Alright?" He looked over at the little girl, "Is that alright with you, dear?"
© 2014 Riot Games, Inc. All rights reserved. Riot Games, League of Legends and PvP.net are trademarks, services marks, or registered trademarks of Riot Games, Inc.


text:
Star Registry Coupon & Promo Codes
Listed below you'll find some of the best star registry coupons, discounts and promotion codes as ranked by the users of RetailMeNot.com. To use a coupon simply click the coupon code then enter the code during the store's checkout process. Learn moreMore »« Less
Current Top Star Registry Coupons
- Coupon Code:Show Coupon CodeStars10SAVE COUPON
- Coupon Code:Show Coupon CodeLCI7602SAVE COUPON
- Coupon Code:Show Coupon Code5AA352BASAVE COUPON
free giftSale
Free Engraved Pendant Necklace or Keyring at Global Star Registry when you place an order.SAVE COUPON
- Coupon Code:Show Coupon CodeDEC15OFFSAVE COUPON
- Coupon Code:Show Coupon CodeFREE SHIPPING SITEWIDE
Free USPS Priority Mail Shipping of All Products to Addresses in the Continental United States. Combinable with other offers.SAVE COUPON
Free USPS Priority Mail Shipping of All Products to Addresses in the Continental United States.SAVE COUPON
- Coupon Code:Show Coupon CodeHALFOFFCGCShared by DiscoDivaSAVE COUPON
Do you want a star? Now you can register a unique Star in your name!SAVE COUPON
- Coupon Code:Show Coupon CodeNASL15
15% off any Name A Star gift set from the webs leading registrar of star names. Gift sets include such features as a star certificate and chart to find your star, a free 120 page astronomy e-book, 6-in-1 astronomy software, and a code to view star via online telescope. Name is also launched to space on a rocket.SAVE COUPON
Top star registry stores
- International Star Registry
- Global Star Registry
- MyRegistry.com
- Gradsave
- Hawthorne & Wren
- ThinkGeek
- Harry & David
- RedEnvelope
- Daily Grommet
- Miles Kimball
- Bonanza
- Novica
- The Black Dog
- Olive & Cocoa
- Bradford Exchange
- MAST General Store
- The Gift Collector
- The Hobbit Shop
- Williamsburgmarketplace
- Pin Mart
- Phi Mu - Official Site
- Always & Forever
- Addoway
- Closeout Zone
- Stardust Memorials
- Wholesale Mart
- Clubs Galore
- The Ultimate Rose
- verbena
- crosbyandtaylor.com/
- Origin of Names
- Earth Angel Oils
- CoasterStone
- UbuyEZ
- GraduationMall
- In the Pink
- Urns Northwest
- Once Upon A Family
- Floracopeia
- LandOfMarbles
- For That Occasion
- RecycleBank
- Arts West Unique Gifts
- Thirstycoasters
- Mookie Gifts
- All Crafts Online
- Abernook
- Pink Olive
- Hinky Import
- The Blushing Apple
Show all 50


text:
November 27, 2014
nick jameson
Gone Too Soon: The Critic
by Jason Hughes, posted Nov 17th 2009 10:12AM
Before The Simpsons begat Futurama, current executive producer of The Simspons Al Jean, along with Mike Reiss, created a short-lived animated series about a film critic who hated almost every film he ever saw.
Starring Jon Lovitz in some brilliant voice work, The Critic ran through two networks in two seasons. Like Futurama and Family Guy, it found some success with reruns on cable -- in this case, Comedy Central -- and a subsequent DVD release. But unlike those series, The Critic remains but a distant memory.
TV Squad Hot Topics
Most Popular Articles
From Our Partners
- 'The Originals' Episode 2.9 Photos: Mommy, Daddy and Hope!
- If I Am Going Down That Dark Alley Then I Want These Characters With Me
- 'The Flash': The Good and Evil from 'Power Outage'
- 'The Vampire Diaries' Winter Finale Photos: Christmas Memories
- 'Criminal Minds' Recap: Rossi's Past Collides With His Present
- More From BuddyTV
- Macy's Thanksgiving Parade 2014: The Rockettes Goof, Scary Newbies & More
- Ratings: Survivor, Hell's Kitchen and Red Band Slip; Charlie Brown Surges
- TVLine Readers Give Thanks for Castle, HTGAWM, Doctor Who, Mindy, NCIS, Freak Show, Once and More!
- Red Band Society's First Season Ending After 13 Episodes; Season 2 Unlikely
- What TVLine Is Thankful For: A Good Surprise, Homeland's Revival, Sexed-Up Murder, Sweet Jane and More
- More From TVLine
- 9 Topics to Talk About During Thanksgiving Dinner (That Won't Start Any Family Fights)
- As New Cosby Accusers Come Forward, Legal Options Remain Limited
- Watch Michael Douglas Sneak Up On His Legendary Dad
- 14 Foolproof Tips For Conquering America's Favorite Holiday: Black Friday
- Chris Rock on Tracy Morgan's Recovery and How ET's Nischelle Turner Inspired 'Top Five'
- More From ET


text:
Learning to measure can be fun time
November 27, 2013 Young children are full of measurement questions. How long is it? How big is it? How far away is it? The answer is hard to explain without knowing measurement units. more »»
Design Build Draft
November 20, 2013 GWINN — Students in Gwinn Area Community Schools' computer-aided design and drafting class are hard at work this year, creating and building a number of things in a hybrid classroom/job site settin. more »»
Children can learn much from animals
November 20, 2013 There is an undeniable bond between humans and animals. A pet can be a friend to talk to, an exercise partner, a protector, a service animal, a lap warmer, and much more. more »»
Escanaba board to seek replacement for resigning member
November 20, 2013 ESCANABA — The Escanaba school board has accepted the resignation of one of its members and authorized the superintendent to put out a notification for interested candidates to fill the vacancy. more »»
Security a continuing focus in local schools
November 13, 2013 MARQUETTE — Area schools are continuing to upgrade their security systems as national headlines call attention to mass shootings across the nation. more »»
Hanukkah, Thanksgiving special year
November 13, 2013 It has been 125 years since Thanksgiving and Hanukkah coincided. This year is a good time to tell children the story of both festivals. For more family teaching fun see and hear grandparentsteachtoo. more »»
With a little help from their friends
November 6, 2013 ISHPEMING — Kids in second grade at Aspen Ridge School in Ishpeming Township are discovering that they read better and have more confidence in their reading when they practice with a furry friend. more »»
Upper Peninsula writers look at copper strike
November 6, 2013 HOUGHTON — Writers and educators from the region and beyond investigated the role of how writing interacts with the working class at this year's Writing Across the Peninsula Conference. more »»
Name is much more than label
November 6, 2013 Names are everywhere. Children learn about their world by giving names to people, places and things. Here is a fun activity to help young children recognize their own and family members' names. more »»
Good drama
October 30, 2013 MARQUETTE — Often when people think about school, images of books and bubble sheet test forms come to mind. more »»
Making kids' candy venn diagrams fun
October 30, 2013 What do young children do for weeks after Halloween? They play with their stash like King Midas with his golden treasure. more »»
Gladstone school board fills board secretary position
October 30, 2013 GLADSTONE — The Gladstone Area School Board filled the vacancy created by the resignation of Bill Milligan accepted last month. The board selected Tom Harrell to fill the post at its meeting recently. more »»
35 and counting
October 23, 2013 MARQUETTE — After 35 years as a teacher, former Marquette Area Public Schools English teacher Karen Angeli has seen it all. more »»
Budget update shows problems for Escanaba schools
October 23, 2013 ESCANABA — Escanaba Area Public Schools shows several early warning indicators of fiscal stress, according to a budget update presented to the school board during its regular meeting Monday by... more »»
Stone soup is great fun for children
October 23, 2013 Have you ever heard of Stone Soup? It's vegetable soup, a great story, and valuable lesson. Children love to cut, chop, and learn to cook soup. more »»
Learning from a master violinist
October 16, 2013 MARQUETTE — Beethoven, Chopin and Handel are moving over this week inside the Kaufman Auditorium, making way for the likes of Zepplin, Kravitz and McCartney. more »»
Fall is family pumpkin, fun time
October 16, 2013 Since family time is often short, how do we make this precious time effective, efficient, fun, and relaxing for all? Any activity like playing with pumpkins can be spiced up with a little learning. more »»
Students take safety training
October 16, 2013 MARQUETTE — Students across Marquette and Alger counties are operating a little safer in their labs and internships after receiving training recently from Parents for Student Safety Employment... more »»
New iPads likely at Apple event scheduled next week
October 16, 2013 CUPERTINO, Calif. (AP) — Apple is holding an event in San Francisco next week to announce new products — likely updated iPads. The company announced its most recent iPads around this time last year. more »»
School safety bill needs only House approval
October 9, 2013 LANSING — A bill that would establish a 24-hour hotline for confidential reports of crimes and threats in schools won Senate approval in June, got funding approved by Gov. more »»
News, Blogs & Events Web


text:
BRING YOUR BIKE and learn how to change your flat tire. This is a great clinic for a refresher or for the first-time cyclist.
Portland State University raises millions for scholarships »
The Oregonian: Portland State professor unlocks virus that could lead to drugs for HIV »
The Oregonian: Oregon's population rises as in-migration accelerates »
go to news
go to events
Ensuring Health - Measuring the benefits of health coverage
go to profiles


text:
Wordsmith Talk
|About Us | What's New | Search | Site Map | Contact Us
You are not logged in. [Log In] Wordsmith.org » Forums » General Topics » Q&A about words » cognomen Register User Forum List Calendar Active Topics Search FAQ
Topic Options
#22931 - 03/21/01 11:39 AM Re: Common cognomens
Carpal Tunnel
Registered: 09/15/00
Posts: 4757
True! Neighboring village of Cilgerran as already mentioned (do a search in the board if you want to see the castle) was the regional centre for slate quarrying. Of course the north Welsh claim to have a couple of little quarries as well, but they're gogs, so we don't talk about them! The name Cilgerran actually refers to slate in itself. I'm sure CK was more right than he knew, though - bet it was a hole lot of fun in the States compared to over here - seems to have done wonders for the genes, anyway
Moderator: Jackie
Forum Stats 8759 Members
16 Forums
13812 Topics
215900 Posts
Max Online: 3341 @ 12/09/11 02:15 PM
Newest Members sstone, BikerVet, MSusanElizabeth, Sumac, cocozh421
8759 Registered Users
Who's Online 0 registered (), 32 Guests and 4 Spiders online. Key: Admin, Global Mod, Mod
Top Posters (30 Days)
wofahulicodoc 114 LukeJavan8 100 endymion6 99 A C Bowden 22 Tromboniator 13 wsieber 1 Storymom 1 tsuwm 1 Jackie 1
Top Posters
wwh 13858 Faldage 13803 Jackie 11610 tsuwm 10524 Buffalo Shrdlu 7210 LukeJavan8 6704 AnnaStrophic 6511 Wordwind 6296 of troy 5400 BranShea 5282
Board Rules · Mark all read Contact Us · Wordsmith.org · Top
Disclaimer: Wordsmith.org is not responsible for views expressed on this site. Use of this forum is at your own risk and liability - you agree to hold Wordsmith.org and its associates harmless as a condition of using it.
Home | Today's Word | Yesterday's Word | Subscribe | FAQ | Archives | Search | Feedback
Wordsmith Talk | Wordsmith Chat
© 2014 Wordsmith


text:
89,166 views
http://booredatwork.com/plantronics-backbeat-go-2-review/ Twitter: http://twitter.com/booredatwork Twitter- http://twitter.com/Black_ironman Twitter- http://...
Plantronics BackBeat Go 2 Review
89,166 views
Plantronics Voyager Legend UC Review
90,351 views
Plantronics BackBeat FIT Review
15,410 views
Plantronics CS50/CS55 Installation Guide
4,752 views
Plantronics VOYAGER EDGE - Smart Bluetooth Headset
18,485 views
Plantronics BackBeat Go Review
168,640 views
Plantronics M55 Bluetooth headset hands-on
68,272 views
[Review] Plantronics GameCom 780 PC gaming headset
120,596 views
I4U News is feeding your Geek Mind. The Geek Mind is concerned with life, in all its different forms and facets.
The geek mind wants to know about societal and financial issues, both abroad and at home. If a Fortune 500 decides to raise their minimum wage, or any high priority news, the geek mind wants to know.
The geek mind wants to know the top teams in the National Football League, or who's likely to win the NBA Finals this coming year.
The geek mind wants to know who the hottest new models are, or whether the newest blockbuster movie is worth seeing. The geek mind wants to know.Read more.


text:
User contributions
For 18.104.22.168 (talk | block log | uploads | logs | filter log)
- 14:59, 1 November 2013 (diff | hist) . . (-3) . . Daylight saving time (change drive in theaters to firework shows)
This is the contributions page for an IP user, identified by the user's numerical IP address. Some IP addresses change periodically, and may be shared by several users. If you are an IP user, you may create an account or log in to avoid future confusion with other IP users. Registering also hides your IP address.


text:
Oh my, this cake is so refreshing and delicious! Celebrate Summer's arrival with fresh strawberry cake topped with strawberry cream cheese frosting. Pureed fresh berries and strawberry extract combine for a lively flavor that is not in the least artificial. Quick, easy, delicious and made from just a few simple ingredients, this strawberry cake recipe is a keeper! Made with our NEW versatile Pure Strawberry Extract. Yum!
- 1 cup butter
- 1 3/4 cup sugar
- 5 egg whites
- 2 teaspoon strawberry extract
- 2 cups pureed fresh strawberries
- 1/2 cup sour cream
- 3 cups cake flour
- 1 tablespoon baking powder
- 1 teaspoon baking soda
- 1/4 teaspoon salt
Strawberry Cream Cheese Frosting:
- 1/4 cup butter-softened
- 1 8oz package cream cheese-softened
- 1/4 cup pureed fresh strawberries
- 1/2 teaspoon strawberry extract
- 6 1/2 cups confectioners' sugar
1. Preheat oven to 350 degrees. Grease and flour three 9″ cake pans.
2. In a large mixing bowl,beat sugar and butter at medium speed with an electric mixer until fluffy. Add strawberry extract,pureed strawberries,and sour cream. Beat until combined.
3. In a medium mixing bowl,sift together flour,baking powder, soda,and salt. Gradually add to butter mixture,beating until combined.
4. Beat egg whites until stiff peaks form. Gently fold into batter.
5. Pour batter evenly into prepared pans. Bake for 30-35 minutes or until a wooden toothpick inserted in the center comes out clean.
6. Let cool in pans for 10 minutes then turn out onto wire racks. Cool completely.
In a large bowl,beat butter and cream cheese with an electric mixer at medium speed until smooth. Add pureed strawberries and strawberry extract beating until combined. Gradually beat in confectioners' sugar until smooth. Frost cake. Garnish with fresh sliced strawberries if desired.
Recipe courtesy of www.olivenation.com.


text:
Well, isn't this spectacular.
Really? Now Mike McQueary can't remember what year he says he saw Jerry Sandusky raping a little boy in the shower?
You'd think the prosecution would have vetted their star witness better than this, but it's becoming increasingly obvious that the principals driving this prosecution apparently went to the Marcia Clark School of Lawyerin'.
After all, on top of their suddenly shaky witness, the state's Attorney General Office submitted a filing that inadvertently leaked the names of some of Sandusky's alleged victims.
Yeesh. Sounds like they could use this guy on the case.


text:
This was not the senior spring Erin Rethlake imagined, with a concussion stealing 3 1/2 weeks from her final turn as Huntington North softball's golden arm in the pitcher's circle.
So she didn't exactly hate giving someone else a headache for a change.
That someone was once-beaten Jay County, and for seven innings Tuesday the Patriots' Larissa Boles and the Vikings' ace took turns abusing the letter K before an aggressive piece of baserunning gave Rethlake all the opening she needed. The Vikings scored the game's only run in the fifth, and Rethlake made it stand up, striking out 15 batters in a three-hit, 1-0 victory in the Homestead Sectional semifinals.
The Vikings will face South Side in the championship Thursday, after the Archers eliminated Wayne 9-8 in the other semifinal. The Archers sent eight batters to the plate and scored two runs in the bottom of the sixth to snap a 7-run tie. Emily Zajac drew a leadoff walk and scored on a fielding error, and Leeanna Washington scored the other run on a bases-loaded walk.
Rethlake, meanwhile, found some retribution in a season that began poorly and got worse with the concussion – until, that is, it turned out to be a blessing in disguise.
I wasn't playing like myself at all, said Rethlake, who's headed for Kentucky in the fall. I'd been pressing too much and trying to do too much at once. So, finally, with (the concussion), I kind of had some time to regroup and figure things out. So when I came back I just came back stronger.
That was obvious Tuesday. Changing speeds and moving her pitches around the strike zone to keep Jay County off balance, she fanned the first five batters she faced, then gave up three straight singles that would have resulted in the game's first run were it not for a critical baserunning error by Jay County's Gabby Hart.
On a soft, looping liner to shallow left field, Hart tried to go from first to third as Katlin Petro scored. But she was extinguished at third for the final out of the inning, wiping out the erstwhile run.
Thus reprieved, Huntington North turned its own aggression on the basepaths into a run, as shortstop Katie Aker bobbled a sharp grounder, then threw late to the plate as Huntington North coach Paris Seibold waved Alexa Hill around from second.
It was one of those kind of plays where you think, She's got a chance,' Seibold said. We're aggressive on the bases, and sometimes that pays off. I had her going as soon as she came around. I thought it was worth a try. A good pitcher like (Boles), you've got to push the run.
After that, Rethlake did the rest, retiring the last nine batters she faced and striking out eight of them. Boles, meanwhile, struck out 11 and allowed just four hits in her second straight outing.
Yeah, I think it's always kind of that way, Rethlake said. My team did what they needed to do, and then it's my job to finish the rest.


text:
Defensive tackle Lance Burlingame, a former UConn commit, signs with Florida Atlantic.
Originally published on
Chuck King
For the second time in a week, FAU has out-dueled Connecticut for a junior college prospect.
Dean College defensive tackle Lance Burlingame signed with FAU on Friday, becoming the second former Dean College player to sign with the Owls this week.
Why did Burlingame end up at FAU instead of UConn?
"It was a whole bunch of stuff," he said. "It just wasn't really going to work out there. With FAU everything was in place. It was a better fit at FAU."
On Wednesday NJCAA All-American wide receiver Lucky Whitehead signed with the Owls, and like Burlingame, did so after being heavily pursued by UConn.
"(Whitehead) told me a lot about the school from his visit and told me he really he enjoyed it. That kind of helped me out," said Burlingame, who has yet to visit FAU.
Burlingame also reportedly had an offer from Purdue.
A 6-foot-4, 290-pound defensive lineman, 11 of Burlingame's 26 tackles as a senior went for loss. Four of them were sacks.
Burlingame possesses unusual quickness for a player his size. In the highlight play of his junior college career, Burlingame successfully sniffed out a screen pass, intercepted it, and raced 40 yards for the score.
A New Bedford (Mass.) graduate, Burlingame recorded 84 tackles, including seven sacks, recovered five fumbles and caused two more.
"I'm excited about coming to Florida," Burlingame said. "I'm sick of all this cold weather."
Burlingame is the fifth junior college player to sign with the Owls during the mid-year signing period. East Mississippi CC QB Quez Johnson, North Dakota College of Science DL Derek Butcher, and CC of San Francisco OL Braden Lyons joined Whitehead in signing their National Letters of Intent to FAU on Wednesday.
All players are expected to be enrolled at Florida Atlantic in January and will participate in spring drills.
Discuss this story in our message boards
Owlballfan, I am not saying the players are D1 or D2 talent. I am asking who rips on young college kids? I would rather spend my money on watching...
More| Reply
I'd like to point out that #12 is second on the team in scoring, third in rebounds, first in steals by a wide margin and is the team's best free...
More| Reply
Please remember these are students trying to get an education and playing sports while they do it.
More| Reply
OK....I will refrain from talking about specfic Student Athlete's....So lets just say #5, #12 and #24 should be playing at a lower level. Better?
More| Reply
Please try and post statements and opinions that are not directed at individual student athletes. They are all playing for FAU and going to school. I...
More| Reply


text:
Online Dating in Beaconsfield for Free
The only 100% Free Online Dating site for dating, love, relationships and friendship. Register
HERE and chat with other Beaconsfield singles
Your mother would approveEnjoy reading,concerts,eating out,dancing to 60's&70's music.Long to see London,Paris,Rome,Barcelona but no fun alone. Would love to meet someone at same stage in life as me, financially independant,g
Beaconsfield Quebec SurferGirl64 63 Woman Seeking Men
Fun, intelligent, love being outdoors.I'm recently divorced, separated for more than five years. I design/create jewellery and hope to start up a website soon (somewhat of a technophobe though). I love to sail, garden and renovate.
Beaconsfield Quebec LeslieElizabeth 49 Woman Seeking Men
Pretend I said something cool here.I'm Zack and I have no idea how to properly start this.I'm the type of person that can equally enjoy doing absolutely nothing and going on some half baked adventure that can go in a billion
Beaconsfield Quebec Theonewithwifi 19 Man Seeking Women
Life is Goodyou spend over 10 years with someone thinking it's a done deal and interests and goals are similar, but they are not. lies told and concealed for long periods, arguments, wishing the good times out w
Beaconsfield Quebec jobearall 34 Man Seeking Women
A Crowded SkyEasy going, great sense of humor, Motivated.....into sports, love music, animals, movies...
Beaconsfield Quebec ChillsComeFromYou 32 Man Seeking Women
MaNaMaNa Be,do,be,do,do........Ma Na Ma Na. Whoa . Sesame Street back flash er, I mean flash back man, Ok , so I remember Old School Sesame Street, it was entertaining, and some what educational, and funny and whacked
Beaconsfield Quebec AuldSchulPunkAllTunesRule 48 Man Seeking Women
Live, Love, LaughI feel like I'm a fun, positive and bubbly person. I love to laugh and to make other people laugh. I just finished my Bachelor's at Concordia in Film. I really enjoy watching films/televisio
Beaconsfield Quebec Krys1017 27 Woman Seeking Men
HERE to use this Free Dating Service, and start contacting other users for free
Looking for companyI am a mature man looking for company. I have time and energy to pursue whatever excites me. I am seeking a lady to explore exciting experiences with me. I will follow you lead into new adventures, an
Beaconsfield Quebec Jupiter2555 59 Man Seeking Women
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30


text:
Gotham Round Table: Take the Cannoliby Sean McKenna at Comments
The battle for Arkham has begun. With Carmine and Sal fighting for control, and Fish plotting in the wings, the world of Gotham continued...Continue Reading
The battle for Arkham has begun. With Carmine and Sal fighting for control, and Fish plotting in the wings, the world of Gotham continued...Continue Reading
It's time for another Round Table, Sleepyheads! This week's Sleepy Hollow Season 2 Episode 4 gave us tons to talk about, as TV...Continue Reading
The reunion we didn't know we were waiting for is coming, Revenge Fanatics! But first, we have to discuss Revenge Season 4 Episode 3...Continue Reading
Sergei Mishnev made an appearance by proxy this week when he sent an assassin to kill a Navy research scientist in Washington in NCIS...Continue Reading
A deal with the devil, an invisible attacker, Ryan with body glitter and Rick and Kate finally getting their groove back all made for a...Continue Reading
Elsa got set up, Marian lost her heart and the Snow Queen has connections to more than just the characters from Frozen. Below TV Fanatics...Continue Reading
Welcome back to the Grey's Anatomy Round Table! Grey's Anatomy Season 11 Episode 3 was all about relationship drama at GSMH,...Continue Reading
Well, Bones Season 10 Episode 3 certainly caused a stir. The controversial installment struck a nerve with many fans, while others found...Continue Reading
Can you believe Supernatural has been on TV since 2005? The show has definitely come a long way now that Dean is a demon and run off with...Continue Reading
Arrow returned last week with smiles, romance and a new baby. But it ended with a devastating loss on Arrow Season 3 Episode 1. Below,...Continue Reading


text:
Siesta Key Villa Rental Photos and Description
2 Bedrooms, 2 Baths, Sleeps 6
Island House Beach Resort- Siesta Key Florida, Crescent Beach - This large villa is approx. 1000 sq. ft. It has 2 bedrooms, 2 baths, large living area, dining area, fully equipped kitchen, all appliances, screened lanai, central ac/heat, covered parking, free wi-fi in unit. The unit has a king size bed, 2 twins, sleeper sofa and only steps to the beautiful blue waters of the Gulf of Mexico. This beautifully maintained complex has 2 beach cabanas, beach chairs, large heated swimming pool, game room, shuffleboard and a scenic beach front courtyard where the sunsets are magnificant. There are many shops, restaurants, fishing, golfing, tennis, boating, parasailing, galleries in the area. Busch Gardens/ Disney are a short driving distance. Come visit us at Island house and let us make your vacation a most memorable one!
- Suitability:
- children welcome
- non smoking only
- pets not allowed


text:
Twin Atlantic Tickets
Get your Twin Atlantic tickets on viagogo, your online ticket marketplace. Choose an event to see the selection of tickets available or check the Twin Atlantic tour on the map to locate the nearest event to you. You can also sell your spare tickets on viagogo, and listing tickets is free. All Twin Atlantic tickets are covered by the viagogo Ticket Guarantee!
Twin Atlantic
The Ticket Marketplace
GuaranteedAll tickets covered by the viagogo guarantee
Your Money Is SafeWe don't pay the seller until you've been to the event
Secure DeliveryDelivered via e-ticket or courier
International Events
|Price from
|La Fleche d'Or, Paris FR Twin Atlantic
Find more like this
Twin Atlantic Tour Tickets
You can buy or sell your Twin Atlantic tour tickets and sign up for Ticket Alerts above to receive updates of new and upcoming Twin Atlantic 2014 tour dates at viagogo. If you have any questions about using viagogo, visit our Frequently Asked Questions page.
viagogo is your online ticket marketplace for concert tickets, sports tickets and theater tickets.
Ticket Alerts
Sign up below for the latest Twin Atlantic news, great deals and special offers.
Your email address appears to be incorrect.


text:
10,298pages on
this wiki
this wiki
Category page
Pages in category "Sports"
" Queensbury Boxing Club is a boxing club advertised on the radio in GTA V. It is unknown if the...
Queensbury Boxing Club
" The U.C.L.S. Bookworms is the College football team of U.C.L.S. in Los Santos in Grand Theft Auto V.
U.C.L.S. Bookworms
" The Liberty City Warriors are a professional men's basketball team in Liberty City in Grand...
Liberty City Warriors


text:
Handpicked by our fashion stylist, you'll find the best of the best here.
We have handpicked the women's 50 best jewellery gifts for you to pursue.
We have scoured the marketplace & collected the 50 top men's gifts.
We have picked the 50 sweetest children's jewellery gifts for you.
Dewdrop Earrings, feature a dewdrop connected by a thread of wire and a delicate tassel chain.
These earrings have been made so that the tassel chain element can be removed, making for a versatile pair of earrings.
Available as 18ct gold plated brass, or 18ct gold vermeil sterling silver.
If ordered in Silver, please allow 14 days delivery as they are made to order.
This product is dispatched to you by De Anna Kiernan
Please note that delivery charges can vary for each designer.
This item usually dispatched in 1 days
This item can be made to order, if none are currently available please add 14 days to the dispatch time.
De Anna Kiernan offers International Shipping.
De Anna Kiernan does not offer Next Day Shipping.
We offer a 30 day no quibble money back guarantee because we always want you to be happy with the items you buy on JewelStreet, and so do our designers! So if you have received an item you want to send back, please log in to your account and contact the seller via the 'My Orders' tab. The only items that are not covered by our guarantee are:-
*Required Fields
Our customers rate our service as excellent
Copyright © JewelStreet 2014
Please wait...