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The invention relates to a baculovirus including a disruption in its endogenous p35 gene and its use in establishing latent baculovirus infections. In some embodiments, the baculovirus can also include a sequence encoding a non-baculovirus RNA and a baculovirus early gene promoter which drives expression of the non-baculovirus RNA.
1. A recombinant baculovirus comprising a disruption in its endogenous p35 gene, a sequence encoding a non-baculovirus RNA, and a baculovirus constitutive promoter that drives expression of the RNA.
2. The baculovirus of claim 1, wherein the disruption is a deletion of a portion of the p35 gene.
3. The baculovirus of claim 1, wherein the non-baculovirus RNA is a mRNA encoding a protein.
4. The baculovirus of claim 1, wherein the baculovirus constitutive promoter is a hsp70 promoter.
5. A method of expressing a RNA in a cell, the method comprising introducing the baculovirus of claim 1 into the cell, culturing the cell, and expressing the RNA in the cell.
6. The baculovirus of claim 2, wherein the portion of the p35 gene comprises the p35 promoter.
7. The baculovirus of claim 3, wherein the protein is a non-baculovirus protein.
8. A method of inhibiting baculovirus superinfection in a cell, the method comprising introducing into the cell a first baculovirus comprising a disruption in its endogenous p35 gene, and exposing the cell to a second baculovirus.
9. The method of claim 8, wherein the disruption is a deletion of a portion of the p35 gene.
10. The method of claim 9, wherein the portion of the p35 gene comprises the p35 promoter.
11. A method of establishing a persistent baculovirus infection in a cell, the method comprising introducing into the cell a baculovirus comprising a disruption in its endogenous p35 gene, and culturing the cell to establish the persistent baculovirus infection.
12. A method for producing a RNA, the method comprising introducing into a cell a baculovirus comprising (1) a disruption in its endogenous p35 gene, (2) a sequence encoding the RNA, and (3) a promoter which drives expression of the RNA; culturing the cell to establishing a culture persistently infected with the baculovirus; and expressing the RNA in the culture.
13. The method of claim 12, wherein the disruption is a deletion of a portion of the p35 gene.
14. The method of claim 13, wherein the portion comprises the p35 gene promoter.
15. The method of claim 12, wherein the promoter is a baculovirus early gene promoter.
16. The method of claim 12, wherein the promoter is a baculovirus constitutive promoter.
17. The method of claim 12, wherein the polypeptide is a non-baculovirus polypeptide.
18. A recombinant baculovirus comprising a disruption in its endogenous p35 gene, a sequence encoding an RNA, and a non-baculovirus promoter that drives expression of the RNA in all stages of the baculovirus life cycle.
19. The baculovirus of claim 18, wherein the RNA is a non-baculovirus RNA.
20. The baculovirus of claim 19, wherein the non-baculovirus RNA is a mRNA encoding a protein.
21. The baculovirus of claim 20, wherein the protein is a non-baculovirus protein.
22. The baculovirus of claim 18, wherein the disruption is a deletion of a portion of the p35 gene.
23. The baculovirus of claim 18, wherein non-baculovirus promoter is a hsp70 promoter.
24. A method of expressing a RNA in a cell, the method comprising introducing the baculovirus of claim 18 into the cell, culturing the cell, and expressing the RNA in the cell.
This application claims priority from U.S. Provisional Application Serial No. 60/094,411, filed Jul. 28, 1998.
Viruses of the family Baculoviridae, commonly known as baculoviruses, are lethal pathogens of insects of the order Lepidoptera. These viruses typically kill their hosts by productive, lytic infection in insect tissues.
Baculoviruses generally contain a covalently closed, circular double-stranded DNA genome of about 90 to 160 kb in length. This genome is capable of accommodating relatively long heterologous sequences. In addition, a high level of structural viral protein synthesis is achieved during productive infection. Consequently, the baculovirus/insect cell system has become a popular protein expression vehicle for academic research and industrial applications. However, since high level expression is coincident with productive infection and late gene expression, the baculovirus expression system is limited by the eventual death of the baculovirus-infected culture.
The invention is based on the discovery that a baculovirus containing a disruption in its endogenous p35 gene is able to establish a persistent infection (i.e., an infection in which the host are not lysed. Consequently, a persistent baculovirus expression culture system can be established by infecting any suitable host cell with a baculovirus having a disrupted p35 gene and an exogenous gene driven by a baculovirus early promoter (i.e., any promoter able to drive baculovirus expression in latent infection).
It was also discovered that cells harboring the p35-disrupted viruses are resistant to baculovirus super infection, i.e., infection by a second baculovirus. Thus, the p35-disrupted baculoviruses can be used to protect insects, especially commercially valuable insects (e.g., silkworms) from pathogenic baculoviruses.
Accordingly, the invention features a baculovirus including a disruption in its endogenous p35 gene. The baculovirus can further include a first sequence encoding a first RNA (e.g., a non-baculovirus RNA) and a baculovirus early gene promoter (e.g., an immediate early gene promoter) which drives expression of the first RNA. The first RNA can be a mRNA encoding a first protein (e.g., a non-baculovirus protein), such as a detectable protein (e.g., βgalactosidase). The baculovirus can optionally also include a second sequence encoding a second non-baculovirus RNA. The second non-baculovirus RNA can be a mRNA encoding a second protein (e.g., a non-baculovirus protein), such as a selectable protein. A detectable protein is a protein whose expression is readily detectable, e.g., by fluorescent, luminescent, or chromogenic assays. A selectable protein is a protein whose expression confers a selectable phenotype on a cell. An example of a selectable phenotype is resistance to an antibiotic or antimitotic drug, such as neomycin or G418. Under some circumstances it can be useful to drive expression of the second protein from a baculovirus constitutive promoter, such as when the second protein confers drug resistance.
A baculovirus early promoter (e.g., a promoter derived from the baculovirus pagi gene) is a promoter, in the context of a baculovirus genome, that can drive expression in the early stage of the virus life cycle, i.e., just after viral entry and before expression of most viral structural proteins. A baculovirus constitutive promoter (e.g., a promoter derived from a heat shock protein-70 [hsp70] gene) is a promoter, in the context of a baculovirus genome, that can drive expression in all stages of the virus life cycle. A baculovirus early promoter or a baculovirus constitutive promoter is not necessarily derived from a baculovirus genome. Promoters derived from the genome of other organisms and viruses are useful in the baculovirus of the invention, as long as they satisfy the above constraints.
The invention also includes methods of expressing a non-baculovirus RNA or a non-baculovirus protein by introducing a baculovirus of the invention into a cell.
The invention also features a method of inhibiting baculovirus super infection in a cell by introducing into the cell a first baculovirus including a disruption in its endogenous p35 gene, and exposing the cell to a second baculovirus. By �exposing a cell to a second baculovirus� is meant placing the cell, or an organism containing the cell (e.g., a silkworm), in an environment suspected or known to contain a baculovirus. The environment can be artificial (e.g., a culture dish to which a baculovirus has been added) or in the wild (e.g., a forest where pathogenic baculoviruses are known to exist). As used herein, the term �inhibiting� includes both complete inhibition and partial inhibition.
A disruption of a gene is any deletion (e.g., deletion of a promoter sequence), insertion, or other mutation in the gene which renders the gene non-functional due to loss or misdirection of transcription, loss or misdirection of translation, or loss of an amino acid sequence necessary for protein function.
The baculoviruses and methods of the invention provide a new eukaryotic protein expression system useful in both academic and industrial settings. Unlike previous baculovirus expression systems, which are characterized by a self-limiting lytic infection, the expression systems made possible by the baculoviruses and methods of the invention utilize persistent, baculovirus-infected cultures. In addition, the baculoviruses described herein can be used to establish cells resistant to baculovirus super infection, a first step in protecting commercially valuable insects from baculovirus-induced disease.
Other features or advantages of the present invention will be apparent from the following drawings and detailed description, and also from the claims.
FIG. 1 is a schematic representation of a region of a baculovirus genome in which the p35 gene has been disrupted and a lacZ and neo gene introduced.
The invention relates to baculoviruses having a disrupted p35 gene. These baculoviruses establish latent viral infections in insect cells, leading to two important results. First, baculovirus persistency can be used to express proteins in persistent cultures. Second, baculovirus persistency blocks infection by subsequent baculoviruses. This latter observation provides a means for protecting commercially valuable insects from baculovirus-induced disease.
Standard procedures for cloning baculoviruses having a disrupted p35 gene and introducing them into cells to establish a persistent infection are well known in the art. persistent infections in lepidopterans can be achieved by feeding insect larvae a diet contaminated with baculovirus having a disrupted p35 gene (see, e.g., Clem et al., J Virol 68:6759-6762, 1994).
Without further elaboration, it is believed that one skilled in the art can, based on the above disclosure and the description below, utilize the present invention to its fullest extent. The following example is to be construed as merely illustrative of how one skilled in the art can practice the invention and are not limitative of the remainder of the disclosure in any way. Any publications cited in this disclosure are hereby incorporated by reference.
Two Autographa californica multiple nuclear polyhedrosis viruses (AcMNPVs) carrying mutations at the p35 gene locus were used in the following example. vAcZΔp35 was constructed as shown schematically in FIG. 1. The lacZ and neo genes were inserted into plasmid pTSV (Lee et al., Nucl Acids Res 22:4683-4689, 1995) to create plasmid pThsN90Z. The neo gene was driven by the Drosophila hsp70 promoter (Steller et al., Mol Cell Biol 6:1640-1649, 1986), while the lacZ gene was driven by the pag-90 promoter, an immediate early type-promoter derived from pag1 of the Hz-1 virus (Chao et al., J Virol 72:2233-2245, 1998). Both genes were flanked by p94 and p35 gene sequences by inserting homologous regions of the baculovirus p94 gene (801 bp, nucleotides −115 to +685 relative to the p94 ATG start codon) and p35 (631 bp, nucleotides +219 to +849 relative to the p35 ATG start codon) into plasmid pThsN90Z to produced transfer vector pTΔ35hsN90Z. These p94- and p35-specific fragments were generated by PCR, and their sequences were confirmed by DNA sequencing.
Homologous recombination was carried out by co-transfecting both AcMNPV genomic DNA and plasmid pTΔ35hsN90Z into TN368 cells. The recombinant virus, named vAcZΔp35, was cloned by screening the transfected TN368 cells for blue plaques with occlusion bodies following X-gal staining and was further verified in infected Sf21 cells by the lack of occlusion body formation. Antibiotic G418 (2 mg/ml) was used in all screenings to eliminate any wild-type virus. The recombinant viruses were further verified by restriction mapping of their genomic DNA and by their induction of apoptosis upon infection in Sf21 cells.
The second AcMNPV used in this example is vAcAnh, called the annihilator, as described in Clem et al., Science 254:1388-1390, 1991. This virus contains a 754 bp deletion in the p35 gene, resulting in a truncated p35 protein which is missing 132 amino acids from its carboxyl terminus.
For complementation studies, the p35 expression plasmid pKih35 hN was created. This plasmid contains a complete open reading frame of the p35 gene driven by the ie1 gene promoter of AcMNPV. A hr5 enhancer sequence of 677 bp in length (Guarino et al., J Virol 60:215-223, 1986) was inserted upstream of the ie1 promoter. The neo gene is driven by the Drosophila hsp70 promoter described above. To produce p35-expressing cell lines, pKih35 hN was transfected into insect cells using CellFECTIN� (Life Technologies). Neomycin-resistant clones were cultured in the presence of 2 mg/ml G418 for two weeks, and three individual clones were isolated.
For infection experiments, S. frugiperda (fall armyworm) cell lines Sf9 and Sf21AE, and Trichoplusia ni (cabbage looper) cell line TN368 were maintained at 26� C. in TNM-FH medium supplemented with 8% fetal bovine serum (Life Technologies). Viruses were each propagated in TN368 cells. Titers of the viruses were estimated by plaque assays using TN368 cells.
When Sf21 cells were infected by the vAcAnh virus or the vAcZΔp35 virus, most of the cells were lysed via apoptosis. The surviving cells gave rise to persistently infected cell clones seven days post-infection. Infection by the wild type virus or by a Cp-iap rescued-p35 mutant vAsB6-1 (Birnbaum et al., J Virol 68:2521-2528, 1994) did not result in persistent clones. Thus, the repression of persistent viral infection is not due to a specific p35 function, but instead is more likely due to a general effect related directly or indirectly to the blocking of cellular apoptosis.
In order to confirm that p35 blocks persistent viral infection, 4�104 Sf21 cells stably transfected with pKih35 hN were infected with vAcAnh at a multiplicity of infection (moi) of 50. At seven days post-infection, viral occlusion bodies were clearly visible, suggesting that functional p35 was produced in these cells in an amount sufficient to complement the defect in vAcAnh. These cultures never yielded persistent clones, indicating that persistent viral infection was dependent on a lack of function for p35 rather than any cis effect of the p35 gene locus in the viral genome.
The capacity of various p35 mutants to establish persistently infected cell clones was evaluated. Parental cells (Sf9 or Sf21) were seeded at a density of 4�104 cells per well in a 96-well plate and infected with Hz-1, AcMNPV, vAcAnh, vAcZΔp35, or vAsB6-1 at various moi. Seven to ten days after infection, the number of surviving cells was determined by trypan blue exclusion. Cell clones containing more than five cells were registered as a viable clone. These cells were propagated to form monolayers where possible. Although some of the clones died during propagation, the number of the original colonies formed by the infection of a certain virus at a certain concentration remained a valid measure of the colony formation potential of the individual viruses.
Only vAcAnh and vAcZΔp35 could generate persistently infected clones. These clones were not observed in cells infected with wild type or vAsB6-1 viruses in a wide range of titers. In these experiments, higher moi generated more colonies suggesting that specific virus gene product(s) enhance the formation of persistently infected clones. Again, this result was consistent with the conclusion that p35 blocks entry into viral persistency.
To establish in culture specific clones persistently infected with baculovirus, 2�105 cells per well in a 24-well plate were inoculated with vAcAnh or vAcZΔp35 at a moi of 50. Two weeks post-inoculation, the surviving cell clones became visible. Clones were isolated, transferred into a 96-well plate, and grown for 7-10 days with the medium changed once every 3-4 days.
The surviving clones were each transferred to a 24-well plate and, if still growing, to larger plates or flasks. On average, about 3-6 clones per 10 original clones survived these transfers. Clones were apparently lost in passage due to apoptosis. These clones were grown in a medium consisting of 50% fresh medium and 50% conditioned medium.
Two groups of persistently infected cell lines were established and used in subsequent experiments. Four persistently infected cell lines derived from infection with vAcAnh were named Sf9-vAc-1, Sf9-vAc-2, Sf9-vAc-3, and Sf21-vAc-1. Three persistently infected cell lines derived from infection with vAcZΔp35 were named Sf9-vAcZΔp35-1, Sf9-vAcZΔp35-2, and Sf9-vAcZΔp35-3.
To detect very low amounts of viral DNA in persistently infected cells, DNA was amplified directly from cultured cells by PCR. Cells were washed twice in phosphate buffered saline (PBS) and diluted to 106 cells/ml. Ten microliters of this diluted suspension were lysed by adding 90 μl of detergent buffer (50 mM KCl, 10 mM Tris-HCl (pH 8.3), 0.1 mg/ml gelatin, 0.45% NP-40, 0.45% Tween-20, 0.6 mg/ml proteinase K). The diluted suspension was then incubated at 60� C. for one hour. After the incubation, the proteinase K was inactivated at 95� C. for 15 minutes.
Ten microliters of this lysate were amplified by PCR and analyzed by agarose gel electrophoresis. Serial 10-fold dilutions of plasmid pThsN90Z were used as molecular standards. The plasmid DNA was amplified simultaneously with the cell lysates to determine the quantity of viral DNA in the cells persistently infected with vAcAnh. Two negative controls, including a reaction mixture without template DNA and a reaction containing only Sf9 cell lysate, were used to ensure no contaminating template in the reagents.
The primers were complementary to the 5′ and 3′ regions of the 801-bp fragment of the p94 gene. PCR products were fractionated on a 1.5% agarose gel and transferred by vacuum blotter (Vacu GeneXL; Pharmacia LKB Biotechnology) onto a nylon membrane. The membranes were probed with a p94 gene fragment labeled with [α−32P] dCTP by random priming (Berhringer-Mannheim).
To determine the presence of viral DNA in cell cultures, total genomic DNAs were purified from uninfected and persistently infected cells and blotted onto nylon membranes using the Hybri-Dot Manifold (Bio-Rad Laboratories). The membranes were probed with viral DNA labeled with [α−32p] dCTP by random priming. The blot was visualized by auto radiography and further quantified using a PhosphorImager (Molecular Dynamics).
Finally, viral transcripts were detected as follows. Total cellular RNA was prepared from different cell clones using Ultraspec RNA isolation reagent (Biotecx). To detect the expression of various immediate early genes, 10 μg of total RNA was reverse transcribed using an oligo d(T) primer and moloney murine leukemia virus (MMLV)-reverse transcriptase. Equal amounts of cDNA were amplified by PCR with specific primers for viral ie0, ie1, and ie2 genes, and cellular gaped genes.
The primers used for PCR were as follows: ie0, GGCAACGCAACATGATAAGAC (SEQ ID NO:1) and GTTCAAGGGTTGCACAGCTT (SEQ ID NO:2), which are complementary to sequences −11 to +720 relative to the ATG start codon of ie0; and ie1, ATCGTGAACAACCAAGTGA (SEQ ID NO:3) and GTTCAAGGGTTGCACAGCTT (SEQ ID NO:4), which are complementary to sequences −22 to +520 relative to the ATG start codon of ie1 (Chisolm et al., J Virol 62:3193-3200, 1988); ie2, AACAGTATCCTACCAGCCCA (SEQ ID NO:5) and CCTCTACTTCTTCTTCGGGT (SEQ ID NO:6), which are complementary to sequences −23 to +612 relative to the ATG start codon of ie2 (Carson et al., Virology 182:279-286, 1991); and GAPDH, GACGGACCCTCTGGAAAA (SEQ ID NO:7) and ACCAGCTGATGAGCTTGAC (SEQ ID NO:8), which correspond to amino acid residues 195 to 310 of the Drosophila melanogaster gaped gene (Lu et al., J. Virol 69:975-982, 1995).
Each PCR was carried out for 30 cycles. Samples without MMLV-reverse transcriptase were also tested to ensure that the fragment was amplified from mRNA. The RT-PCR products were separated by electrophoreses on 1.2% agarose gels and transferred to a MAGNA nylon transfer membrane (MSI) using a vacuum blotter (Vacu GeneXL; Pharmacia LKB Biotechnology). The membrane was hybridized separately with 32P-labeled probes against ie0, ie1, ie2, and gapdh genes at 60� C. overnight in hybridization buffer containing 0.25 M Na2HPO4 (pH 7.2), 1 mM EDTA, 7% SDS, 1% BSA fraction V, and 10% formamide.
In all the persistently infected cells infected by vAcAnh, viral DNA was detectable by PCR, followed by Southern hybridization after 80 passages. After prolonged serial passages, still higher levels of viral DNA content were detected by Southern analysis in Sf9-vAcZΔp35-1, Sf9-vAcZΔp35-2, the vAcAnh-infected cells. Dot blot hybridizations confirmed this general trend.
In addition, the expression of viral early genes was monitored using RT-PCR. The expression of these early genes was detectable in Sf9 cells initially infected by the wild type AcMNPV. Early gene expression was also apparent in cells newly infected with the annihilator vAcAnh before latency was established. No early gene expression was detected in cells persistently infected by vAcAnh. In contrast, most of the viral early genes were expressed in persistent cell lines Sf9-vAcZΔp35-1 and Sf9-vAcZΔp35-2 established by vAcZΔp35. The expression of all the tested early genes from these two cell lines were relatively strong and not distinguishable from the early gene expression of cells productively infected by wild type viruses, suggesting that the viral genome was maintained in an inactive state within the infected cells. It was also believed that expression of exogenous p35 could release the block to productive infection; this supposition was tested as described below. The expression of early genes was not detected in Sf9-vAcZΔp35-3 cells.
To determine whether β-galactosidase was expressed in cells infected with vAcZΔp35, cells were fixed for five minutes at room temperature in a solution containing 2% formaldehyde, 2% glutaraldehyde, 150 mM NaCl, and 15 mM Na2HPO4. Cells were then washed twice with a solution of 150 mM NaCl and 15 mM Na2HPO4, and stained with a solution containing 1 mg/ml 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, 5 mM K3Fe(CN)6/K4Fe(CN)6, and 5 mM MgCl2. The cells were stained overnight at 37� C. before microscopic examination for blue cells, which are indicative for lacZ expression.
Essentially all infected cells exhibited initial lacZ activity after infection with vAcZΔp35. During early passages, some 10%-20% of the cells died via apoptosis. At passage 5, the percentage of total cells in culture that were apoptotic began to decrease, while about 50% of the cells expressed lacZ. These persistently infected cells grew slower than did the parental cells, with a doubling time of roughly 27-30 hours. The lacZ activity was observed for 50-60 passages over a period of five to six months.
Parental and persistently infected cells were challenged with either vAcAnh or wild type AcMNPV at passage 90. Both the parental cells and persistently infected cells were challenged with AcMNPV or vAcAnh at an moi of 10, 1, and 0.1. After adsorption, the residual viruses were removed, and the cells were incubated with culture medium at 26� C. for three days. Viability of the cells was estimated by trypan-blue exclusion.
Sf9 and Sf21 cells were fully susceptible to challenge, even at an moi of 0.1, as indicated by the near total death of the culture three days after challenge. Partial resistance to the infection of these two viruses was observed in Sf9-vAc-1 and Sf9-vAc-2. A challenge at a moi of 10 resulted in close to 100% death, the challenges at an moi of 0.1 led to only about 10% dead cells after three days. Resistance to challenge was far less evident in Sf9-vAc-3 and Sf21-vAc-1, though a significant reduction in the percentage of dead cells, relative to Sf9 and Sf21, could be seen at a moi of 0.1.
Significant viral resistance was observed in cells persistently infected with vAcZΔp35. In general, less than 5% of the cells were dead three days post-challenge at any of the above moi. Clearly, baculoviruses with disrupted p35 genes could inhibit super infection by lytic baculoviruses.
No infectious virus could be detected in Sf2l-vAc-1, Sf9-vAc-1, Sf9-vAc-2, or Sf9-vAc-3. Low viral titers could be detected in the cultures of two persistent cell lines, Sf9-vAcZΔp35-1 and Sf9-vAcZΔp35-2, but not in Sf9-vAcZΔp35-3 cells.
To further study whether infectious virus could be reactivated from these persistently infected cells, we transfected p35 expression vector pKih35 hN into the persistently infected cell lines. Persistently infected cells (2�105 cells/well in 24-well plates) were transfected with 1 μg of plasmid pKih3 hN as described above. As a control, parental Sf9 cells were transfected with pKih35 hN. At 24 hours post-transfection, these cells were infected with vAcZΔp35 at a moi of 10. The culture media were harvested at six days post-transfection, and the titers of released viruses were determined by a plaque assay using TN368 cells.
The positive control culture resulted in abundant production of infectious virus. Yields of viral progeny from Sf9-vAcZΔp35-1 and Sf9-vAcZΔp35-2 cells transfected with the p35 expression plasmid were increased, although not dramatically on a log scale. On the other hand, the yield of virus from Sf9-vAcZΔp35-3 cells transfected with the p35 expression plasmid was dramatically increased by several logs. Another non-virus-producing persistent cell line, Sf9-vAcZΔp35-4 (established at a date later than that for the first three clones) yielded abundant virus after transfection of the p35 expression plasmid.
These observations indicate that the persistent baculovirus-infected cells were genuinely latent, since production of viruses could be triggered by expressing p35, thereby releasing the block from latency to productive infection.
It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of this invention.
For example, although the disruption of the p35 gene and the introduction of one or more non-baculovirus genes are achieved by one single replacement of a baculovirus genomic sequence, other genomic modifications are within the scope of the claims. The introduction of a sequence encoding a non-baculovirus RNA or protein can be at any position within a genome that already has a disrupted p35 gene.
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I have a mandatory mediation in two weeks. I do have an attorney. My Dr's deposition was last week, have not heard anything so, I'm guessing all went well. But, do insurance companies normally like to settle prior or during mediations?
Since you have indicated that you do have a Attorney, this question is best posed of your own counsel.
I would not assume anything regarding how well your Deposition went, and again, I would inquire of your Attorney as to what transpired during the course of the deposition and what effect, if any, it had upon your case.
As noted above, the purpose of the Mandatory Mediation Conference is to attempt to facilitate settlement between the parties. My experience has been that the Mandatory Mediation Conferences are far less successful than Voluntary Mediation Conferences.
You should contact your Attorney to discuss what the status of negotiations is prior to the Mediation Conference and further, to discuss what you can expect to occur during the course of same. Please keep in mind that although the Mediation Conference is mandatory, it is not binding and you're not required to sell your case during the Mediation Conference if you're not happy with the Defendants offer.
This question really should be addressed to your attorney. I agree with the others that you should not assume anything about how the deposition went...you should ask your attorney. In my experience, cases that have been assigned a mediation date usually do not settle until mediation. It is possible to settle sooner, but it is not as common. Call your attorney and have him/her answer all of your questions.
The point of a mediation is to enter into a discussion and negotiation to settle a claim, with the mediator playing a central role in seeking a consensus among the parties. In my experience it would be unusual for an insurance company and my client to resolve a matter on the eve of a mediation, but a consensus can be reached at any time. You should discuss your upcoming mediation with your lawyer, who can provide better insight on your specific circumstances.


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FORT MYERS, Fla.-The new "Thrifty Mama" boutique will celebrate its grand opening weekend on January 28-29 with special giveaways and sales. The thrift boutique is located at 1952 Park Meadows Dr., Fort Myers, about 1 mile north of Bell Tower off the west side of US 41. The boutique is open Tuesdays - Saturdays 10 am to 6 pm.
Since 1992, Healthy Start has been serving Lee, Collier, Glades and Hendry counties. The non-profit organization works to reduce the number of infant deaths, reduce the number of premature or low birth babies and improve the health and developmental outcomes for young children.
For more information visit www.HealthyStartSWFL.com or www.ThriftyMama.org.


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Fall of Giants: Book One of the Century Trilogy (original 2010; edition 2012)
by Ken Follett
Work details
Fall of Giants by Ken Follett (2010)
Best Historical Fiction (188)
World War I Fiction (23)
Historical Fiction (199)
Compact | Rate recommendations
No current Talk conversations about this book.
I liked this book. It was a fun historical read.
Okay, so the not so great.
It took me until the end to realize he was setting all these characters up together so we could have a sequel. But it really felt forced. Like two characters awkwardly fell in love, there was heavy petting and sex forced into two paragraphs, and then moved onto the history. I love a good romance, but I really wished it had been less forced.
Still, the history was good.
All in all, 3 stars. Not great. Not terrible.
I know, based on experience as a reader these three years, that whatever I say about Fall Of Giants will not result in an insightful review. I don't have the reading history to back up my claims and my inclinations. I just think that what comes to mind with this book is that there's nothing new under the sun, especially Fall Of Giants.
I have read very few writers of historical fiction. Not only that, but FoG is the only Ken Follett book I've read. I can only compare him to Jeffrey Archer. In my mind, Archer is head and shoulders above Follett. Follett's shallow interpretation of his characters means that when it comes to milking drama for the pay dirt of the reader's (THIS reader's) emotions, his attempts ring hollow.
There are sparsely dotted examples of poignant thoughts by the characters. The author didn't give the required pathos to his cast. These people comprise of fictional and real people. Both sets are dead. The book ends in 1923. That's a year before my late grandfather was born. So we're viewing another generation from a different world. The author didn't capitalize on all the possibilities. Did the author trust his audience to go along his one dimensional creation? His experience, in terms of accolade and success for FoG, has been validated. Quite a pity. FoG is just a boring book that will sooner or later go out of print. Maybe future readers will be spared the possibility of reading it. One can only hope.
Couldn't keep this down. It's not as great as Pillars of the Earth, but it's great regardless.
I have to fess up and admit I only read the first third of the book. And I can't help but disappointed. I used to love Follett when I was 30 years younger. I think I read everything he'd published up to that date. And the reason was he was so EASY to read and he wrote with such interesting information about his topics. I learned more about cathedral construction from Follett than anyone else.
But times make ancient good uncouth and these days I am looking for more challenge from my books and get a bit tired of everything so neatly laid out that I don't have to put much effort in. This is hardly Follett's fault, of course. His scrupulous talent for plotting his mass of characters and helping the reader to never lose the thread is a feature of his writing. But I need a bit more these days not to get bored. Sorry for me, eh?
Ken Follett is a very good writer, although the same themes repeatedly appear in his books (like in [b:The Pillars of the Earth|5043|The Pillars of the Earth |Ken Follett|http://photo.goodreads.com/books/1309285683s/5043.jpg|3359698]): the privileged and powerful do not deserve their power, those in power may be good people but their positions lead them to abuse their power, the privileged believe they deserve their privilege, those who have no power must do anything possible to survive. He also likes to reuse the same "surprises," so we are "shocked" to find out that so-and-so is the secret/long-lost son/daughter of the earl/king, etc. However, I enjoyed this book for its insights into the period leading up to and including World War I.
Trotz peinlicher Sexszenen auf Groschenromanniveau und wie Untertassen dahinfliegender Dialoge: Ken Folletts neuer Roman ist gut recherchiert und freundlich-sozialdemokratisch - einer Verfilmung im Öffentlich-Rechtlichen steht nichts im Weg.
Die Aufteilung von erfundenen Schicksalen und weltgeschichtlich verbürgten Ereignissen löst Follett perfekt.
Overall, Follett is masterly in conveying so much drama and historical information so vividly. He puts to good use the professional skills he has honed over the years — giving his characters a conversational style neither pseudo-quaint nor jarringly contemporary. That works well. And for all his belief in the redemptive quality of liberal humanism, he makes sure not to endow his characters with excessively modern sensibilities. As for the occasional cliché — well, unless you're Tolstoy, you're not going to have the time or the ability to be original throughout your 1,000-page blockbuster. Ken Follett is no Tolstoy, but he is a tireless storyteller, and although his tale has flaws, it's grippingly told, and readable to the end.
Despite all this, "Fall of Giants" offers pleasures that more than compensate for its lack of literary finesse. Follett may not be Tolstoy, but he knows how to tell a compelling, well-constructed story. Once its basic elements are in place, the narrative acquires a cumulative, deceptively effortless momentum.
A lot happens on the first page of Ken Follett's "Fall of Giants." King George V is crowned at Westminster Abbey. A Welsh boy named Billy Williams turns 13 and begins his wretched life as a coal miner. And Mr. Follett, who was once a Welsh boy himself but grew up to become his generation's most vaunted writer of colorless historical epics, kicks off a whopping new trilogy. His apparent ambition: to span the whole 20th century in blandly adequate novels so fat that they're hard to hoist.
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Follows the fates of five interrelated families--American, German, Russian, English, and Welsh--as they move through the dramas of the First World War, the Russian Revolution, and the struggle for women's suffrage.
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The Boska Petit Paris Cheese Board features an integrated wire cutter. The cheese serving platter also comes with a lid.
The most important things to know when shopping for a camera.
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Want to be more of a player on Twitter? Don't be afraid to say the same thing four times a day, the investor and former Apple evangelist advises.
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NBA star visits Browns camp Aaron Wilson
Cleveland Cavaliers superstar LeBron James visited the Cleveland Browns' training camp today.
He chatted with his friend, rookie quarterback Johnny Manziel.
"When you have one of the biggest athletes in the world at your practice, there has to be a lot of buzz,'' safety Donte Whitner said. "A lot of guys never met LeBron before, so it's exciting.''
Manziel met with Browns coach Mike Pettine, who introduced the NBA player to his daughter.
"I'm going to be father of the year now,'' Pettine said. "I got my daughter a picture with LeBron."
Several players posted for pictures with James.
"It's pretty cool,'' Whitner said. "All eyes should be on Cleveland, and now we have to go out there and make people want to watch us. The Cavs go out there and handle their business, we handle ours, and (hopefully we can) make people understand that Cleveland is pretty much back."
Follow me on Twitter: @RavensInsider
Aaron Wilson covers the Ravens for The Baltimore Sun
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Welcome to revolution corner, or 'The only spot on the interlink to buy one-off die-cut stickers'. Individual slices of personality, totally unique and 400% awesome. A splendid way to collect t-shirt designs when your wardrobe is full.
*Not actually crazy, actually true.
Printed to fit best inside a 3" x 4" window, including 3mm white padding around the edge.
Inspired by the sprites in the original Final Fantasy series, this set includes the Fighter, Monk, Theif, White Mage, Red Mage, and Black Mage!
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Sylosis's latest setlist
Underground (Cologne) 27-03-2014
Fear the World, Stained Humanity, ?, Teras, EmpyrealSylosis setlist on setlist.fm
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Cadillac CTS Questions Maintenance & Repair
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This page contains Cheats for Russian Durak organized by sections for Android. Russian Durak is a Miscellaneous Board Games game, made by Symetra Applied Technologies, released on Dec 3, 2010 and published by Symetra Applied Technologies. If you can't find a hint or secret in our list, then please check this page periodically for the latest updates.
- Developer:
Russian Durak Android Cheats
Russian Durak (Android) is a game of chance and wit. The object of the game is to get rid of all one's cards and at the end of the game the player with cards in their hand is Durak. If you like solitaire, you will love this game. Russian Durak (Android) has Startup 3d shading Tool-tip formatting, and support Podkindoi durak Sorting.Symetra Applied Technologies
- Publisher: Symetra Applied Technologies
- Genre: Miscellaneous Board Games
- Release Date: Dec 3, 2010
- ESRB: Not Set
Stuck on this game? Set a specific, clearly defined question and let others answer your question or view answers to previously asked questions. If you think you are an Russian Durak expert then please try to help others with their questions.
Don't forget to Bookmark this page for the latest Russian Durak Cheats. At this moment there are no cheats or walkthroughs available for Russian Durak. If you know cheats, secrets, hints or glitches for this game then please Submit them.Submit Cheats
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A lot of you know that in my blogs I write about extreme hunting and fishing adventures, but before any person goes offshore in search of Sailfish and Tuna, or to the Great white North of Alaska for Bear or Moose, there was a little kid with a cane pole or a crab trap complete with a chicken leg tied to the middle in search of blue claw crabs and sand crabs. Hurricane Sandy, which recently devastated parts the North East, also destroyed many of the areas that I grew up in, including one of my favorite bridges that I fished and crabbed from as a boy. I often wonder why that in today's difficult economic times, and with the need for some easy relaxation, I don't get back into some of those adventures I held so dear in my memories of childhood. So with that I have decided to begin once again hunting the bays and bridges of South Florida in search of the elusive Blue Crab.
In this article, I will tell you how to catch blue crabs, and then, how to cook them!
Crab remains have been identified at paleolithic camp sites in areas of the Suwanne river, and at Archaic sites as far south as 10,000 islands, so we know that Floridians have been chasing these tasty monsters for at least 12,000 years or so. We've learned to eat them in everything from butter to hot sauce to spaghetti sauce, so your own preference is bound to produce a wonderful dinner. They're relatively easy to catch, too.
Many of us that were born, or spent their childhood up North know very well about Blue Crabs (or Blue Claws), but we seem to have stopped looking for them once we hit South Florida. Well, Guess what? There are quite decent amounts of those tasty crustaceans down here too! You just need to know where to look. They can be found on both the Atlantic and Gulf coasts, but remember, the sandy beaches are not the optimum place to find them, as there are fewer places for them to hide to avoid predators such as Tarpon and Permit. Look for grass beds that not only provide cover for the Crabs but also an ample supply of food. The pilings of bridges are also a great place to look, and you can do this scouting by boat or by foot. Areas near the Skyway Bridge towards Tampa and the Card Sound Road heading towards Key Largo have both proven very productive.
Traps and Florida Blue Crab Regulations (Recreational)
After checking Florida blue crab regulations for how many traps you can use and what license is needed (if any), you can go to your local tackle shop or Outdoors superstore (even Wal-Mart might have them) and purchase a trap or two. When fishing from shore I usually stick with the Pyramid shaped Crab Trap. Basically you tie a chunk of bait to the center of the trap and when it is lowered to the bottom the walls of the pyramid open and lay flat on the bottom, allowing Crabs to walk up to the bait to start feasting. After about 45 mins to and hour you quickly pull on the trap line, closing the Pyramid with the crabs trapped inside. Pull it in quickly as quite often there may be some crabs hanging on the outside of the trap. I have seen guys whip this trap around to get some serious distance from the dock or bridge they are fishing from, but its really not all that necessary.
Another type of trap that is used is made of wire mesh in the shape of a big box or rectangle. These can be used from a boat and left for much longer periods of time. You can leave them out by tying a float to the end of the line and then coming back in several hours or even a day or two. In these types of crab traps, the Blue Crabs will walk in and not be able to get back out do to the design of the entrance. This type of trap is the one where you should really check the state license requirements for crabbing, as I think the non-commercial limit is five traps. Also, be mindful to release any crabs that are carrying eggs on their bellies and it's always a good idea to release small ones.
What Do Blue Crabs Eat?As for bait, Crabs are pretty much scavengers and almost anything will work. I have seen everything used from Chicken parts to other things you can get at a grocery, but lets try to keep it to what they usually eat. Next time you go out fishing, freeze up what you are not keeping, a fish head, a dead Mullet or any other oily fish, if its got scent, it can draw in crabs. Make certain you attach whatever you are using for bait securely to the center of the trap, crabs can leave you sitting with an un-baited trap for hours before you realize they walked off with the bait. Once they get a hold of the bait they will hold on and guard it with a passion. I have seen a young man tossing out just a line with a chicken leg attached, and then bringing it up with a Blue Crab hanging on right to the waters surface, and then scooping it up with a dip net. Consider this method number 3 for catching them.
How to Cook Blue Crabs
So now you have a pail full of Blue Claw Crabs, one of the seas most delectable treats. What next? Well, whether you are from New England, Chesapeake Bay or the Gulf Coast of Texas, its pretty much always been the same, toss your crabs into a big pot of boiling water or steam them, but make sure they are alive when you begin and season the water as you like it, I'm an Old Bay Seasoning kind of guy, but pretty much any kind of fish boil seasoning will do just fine. Wait for the shells of the crabs to turn wonderful shade of vivid red, pick a crab and whack it with a mallet until you can get to the sweet white meat of the claws and body of the crab. It can get messy, but its oh so worth it!
Whether you are reliving your youth, merely looking for an economical way to get out and have some fun with the kids, or entertaining friends visiting from the Midwest who have not seen an ocean, A quick trip to the bait and tackle store and a bit of scouting can not only amuse you, but create a great feast that all can take part in harvesting and cooking! Good Crabbing and Bon Appetite!
From the publisher: This "How to Catch Blue Crabs" article is the second of a series of "how-to" articles from an outdoor writer named Noah Hochman that we were lucky enough to stumble upon. Noah has a beautiful, informative website called TheOutdoorsGuy.com, where you can find how-to articles, as well as great stories about everything outdoors: fishing, hunting, hiking, etc. Give his site a visit, you'll be glad you did. Thanks for sharing your great stories and knowledge with us Noah!
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The Johnson Rose 63212 12" X 1" Deep Dish Pizza / Cake Pan With Beaded Edge is great for baking mouth-watering deep dish pizzas or cakes. This 0.039 inch thick pan ensures even heat distribution, creating perfect golden crusts every time. Made from durable and long-lasting 18-gauge aluminum, this pizza pan can withstand the demands of any commercial kitchen. Deep Dish Pizza/Cake Pan, 12" x 1", Straight sided with a beaded edge, 18 gauge hard aluminum
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McDonald's Holiday Lights at the Beach - Look below to save $8, plus military discounts.
Santa...in the sand?
Rolling out Nov. 21-Jan. 3, this event a magical family fun attraction. Located at the Virginia Beach Boardwalk, it's the perfect way to start the holiday season. Join 30,000 people who flock annually to this festive and creative light display on the oceanfront.
Don't worry--that's 30,000 over the entire holiday season--you won't have to battle them all at once! Still, go with what the locals know: Saturday nights have the biggest crowds, so stick with Friday and Sunday nights.
Here's something else the locals know: It's one of the holiday highlights of the year. In fact, it's been named one of the Southeast Tourism Society's "Top 20 November Events" the last few years. Some of the top sights at this extravaganza include a "Twelve Days of Christmas" light exhibit, a three-story Christmas tree of lights, and a "surfin' Santa."
This year's show-- sponsored by Liberty Tax Service-- is your once a year chance to actually "cruise" the boardwalk. (Note: There is an entrance fee for cars, but you can cram as many people into your car as your like!) This fee includes a free CD featuring music to light up your magical journey down the dazzlingly lit boardwalk.
When: Sunday-Thursday, 5:30pm – 10:00pm Friday-Saturday, 5:30pm – 11:00pm
Location: Virginia Beach Oceanfront, 2nd Street to 34th Street (enter at 2nd street)
Method: Your Car!
Cost: $15 per car Monday-Sunday, Thanksgiving and Christmas Day
Limos $25; Motor coaches $45; Mini Buses $25
$25 Combo Tickets (Holiday Lights and Norfolk Botanical Garden's Garden of Lights)
Opening Weekend Deal - $8 Friday, Nov. 21-Tuesday, Nov. 25.
Special Military Discount! - On Mondays, December 31-Jan. 3, active military pay just $7 per car (must show military ID)
None are posted yet, but last year I found a coupon good for $2 off, so keep checking back!
So c'mon...come see Santa at the beach. It's just one more reason Virginia Beach is such a great family vacation destination...year-round.
Special Events for 2014 -
Walk through the Lights for Ronald McDonald House Charities Norfolk - Wed, Nov. 19
From 5 pm to 8 pm, make a donation at 2nd street and you can enjoy a rare chance to walk (and not drive) through the lights, while supporting the Ronald McDonald House Charities.
Looking for more holiday fun, including light events, parades, ice skating, a winter planetarium, and more?
Check out these links:
For November & December holiday events, click here
For our top three favorite holiday itineraries, click here
Nov 24, 14 08:11 PM
The best Christmas Town deal of the season may be over, but you can still get $10 off Busch Gardens Williamsburg's Christmas Town (open this weekend for its second weekend of the season) when you use…
Nov 24, 14 08:04 PM
Christmas Town at Busch Gardens - 2014 Hours, Discounts, Ways to Save Money, What to See and What to Skip, Best Places to Take Photos
Nov 22, 14 07:16 PM
Join a beloved holiday tradition by taking a special Holiday Candlelight Tour at George Washington's Mount Vernon. Tours begin Nov. 28 and run through Christmas. In addition to touring the mansion, gr…


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Minneapolis emcee Rocky Diamonds has come together with The DJBooth to bring listeners an exclusive pre-release stream of his latest mixtape, ArtOfficial.
The 17-track project, which is presented by Paperroll$ and FakeShoreDrive.com, includes previously-released song selections "Day One," "Crazy" and "21." Guest features are kept to a minimum on ArtOfficial, with August Alsina and Uncle Skoot making the lone appearances on the set. Meanwhile, production muscle is handled by a wide range of established and new school producers, which include Anonymous, Cardo, Chinky P, J. Classic, $K, Sonny Digital, TrakkSounds and more.
Fans can also check out Rocky Diamonds's previous albums: Rocky Diamonds - LONER | Rocky Diamonds - The Marckus Shaw EP
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Romo, wife expecting first child
Dallas Cowboys quarterback Tony Romo revealed Tuesday morning that he and his wife are expecting their first child.
Romo and Cowboys wide receiver Miles Austin were at Cedar Hill High School in Cedar Hill, Texas, to address students as part of the school's Red Ribbon drug prevention campaign, when he revealed the news.
When a teen asked Romo whether he has any children, the quarterback replied: "I got one on the way. My wife's pregnant."
Romo and former Miss Missouri and sports anchor Candice Crawford were married in Dallas in May.
Coincidentally, pregnancy rumors surfaced this month for Romo's former girlfriend, singer-turned-fashion-designer Jessica Simpson, who has been photographed recently with a prominent baby bump.
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83-15693 - 2TB 3.5" Desktop Hard Drive 7200RPM
The DT01ACA series is Toshiba's first 3.5-inch hard drive for use in power-friendly consumer and commercial desktop computers.
298-0010 - AAA Heavy-Duty Alkaline Batteries - 40pk
40-pack Ultra Alkaline AAA Batteries
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Hamilton reveals residential skyscraper for Elephant
Hamilton Associates has submitted proposals for a £100 million energy-efficient tower in London's Elephant & Castle for planning.The 25,000m 2development - complete with integrated wind turbines - will have 42 floors, 310 private apartments and include affordable housing, if it gets the go-ahead. At the top of the building will sit three 9m-diameter wind turbines, able to produce enough electricity to light the entire building, according to developer Multiplex. The developer claims that the uppermost 20m of the 147m-tall building would house this 'energy wind farm', a 'highly visible representation of the building's green credentials', if it is approved by Southwark Council planners. Multiplex director Richard Banks said: 'We have created a pioneering, landmark building of the highest quality that will be the first of a cluster of towers that marks the new Elephant & Castle.' by Rob Sharp
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8 results for "kieran darcy"
Segal to make Rangers debutKieran Darcy
GREENBURGH, N.Y. -- With winger Arron Asham suspended for the Rangers' regular-season opener, Brandon Segal has been called up from the Connecticut Whale and will be with the team Saturday in Boston. Segal, 29, made his NHL debut with the Tampa Ba...Blog | January 17, 2013
Kreider ready for first regular seasonKieran Darcy
GREENBURGH, N.Y. -- Chris Kreider scored five goals for the New York Rangers in last year's playoffs, as a rookie -- tied for third on the team. Yet the 21-year-old winger's primary concern right now is his job status. "Just try to make the team f...Blog | January 17, 2013
Salvador named Devils' captainKieran Darcy
The New Jersey Devils on Tuesday named Bryce Salvador their captain, the 10th in team history. The 36-year-old defenseman has been with the Devils since 2008 and replaces former captain Zach Parise, who signed with the Minnesota Wild in the offsea...Blog | January 17, 2013
Rangers focused on the power playKieran Darcy
GREENBURGH, N.Y. -- In their second-to-last practice before the start of the regular season, the Rangers worked on the power play for the first time. With good reason -- it could be the key to winning the Stanley Cup. The Rangers were tied for ...Blog | January 17, 2013
Nash: 'This is what I dreamed of'Kieran Darcy
New Rangers winger Rick Nash was a guest on "The Michael Kay Show" on ESPN New York 98.7 FM on Wednesday, three days before he is scheduled to make his debut in the team's regular-season opener against the Bruins. Listen to the complete interview:...Blog | January 16, 2013
Lundqvist: 'This year is a little special'Kieran Darcy
GREENBURGH, N.Y. -- The New York Rangers have been pegged as one of the favorites to win the Stanley Cup this season -- and they know it. "Playing here in New York, you always have to deal with pressure and expectations," goalie Henrik Lundqvist s...Blog | January 16, 2013
Richards returns to the iceKieran Darcy
GREENBURGH, N.Y. -- Brad Richards, the New York Rangers' first-line center and alternate captain, returned to action Wednesday after missing the first three days of training camp with the flu. The Rangers did not skate as a team on Wednesday, but ...Blog | January 16, 2013
These memories still burnKieran Darcy
Getting to the postseason is not enough -- just ask Atlanta fans. Here are Atlanta's most painful sports moments.Story | Conversation | May 04, 2004


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Roman Soler by Nameeks Drako Round Shower Column
Ramon Soler's Drako double handle (small lever handle style), 2 hole shower column is a shower column that will fit perfectly into a modern style bath. Imported by Ramon Soler, this wall shower column is a luxurious option for your upscale master bath. Match all your other chrome bathroom fixtures with this decorative chrome shower column, made in very high quality brass.
Roman Soler by Nameeks Drako Round Shower Column
Additional Documents
Weights & Dimensions
Slide Bar
Hand Shower
Additional Weights & Dimensions
|Product Type: Complete Shower System
|Shower Head Type: Slide Bar Shower Head
|Style: Modern
|Finish: Chrome
|Hardware Finish: Chrome
|Gloss Finish: Yes
|Material: Metal
|Waterway Material: Metal
|Hardware Material: Stainless Steel
|Number of Items Included: 5
|Pieces Included: Diverter and rough, slide bar, hand shower, and hose
|Rust Resistant: Yes
|Chip Resistant: Yes
|Tarnish Resistant: Yes
|Stain/Spot Resistant: Yes
|Drip-Free: Yes
|Faucet Type: Cartridge
|Cartridge Material: Wax
|Temperature Memory: Yes
|Adjustable Water Flow: Yes
|Shower Arm Included: Yes
|Detachable Shower Head: Yes
|Hand Shower Included: Yes
|Hand Shower Hose Included: Yes
|Shutoff Valve Included: No
|Rough-In Valve Included: Yes
|Valve Trim Included: Yes
|Transfer Valve Included: Yes
|Number of Outlets: 2
|Outlet Type: Male
|Number of Inlets: 1
|Inlet Type: Male
|Pipe Thread Size: 14
|Diverter Type: Trim With Diverter
|Body Jets: Yes
|Electronic Control Unit Included: No
|Faucet Handle Included: Yes
|Slide Bar Included: Yes
|Soap Dish Included: No
|Installation Type: Wall Mounted
|Drain Assembly Included: No
|Commercial Use: Yes
|Eco-Friendly: Yes
|Product Care: Do not use strong abrasive cleaners
|Country of Manufacture: Spain
|Assembly Required: Yes
|Additional Parts Required: No
|Product Warranty: 1 year limited
|ADA Compliant: No
|WaterSense Compliant: No
About the ManufacturerA line created by Nameeks, brand is a concept based on simple lines and natural materials, managing to convey a feeling of serenity, luxury, cleanliness, modernity and comfort, creating a pleasant tone in the bathroom. Submerge yourself in the fascinating luxury world of Nameeks Total Bath Solutions.
Roman Soler by Nameeks Drako Round Shower Column
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Roman Soler by Nameeks Drako Round Shower Column
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OUR SKU # ROMS1090Drako Round Shower Column is also sometimes listed under the following manufacturer numbers: Ramon Soler US-3396Z


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Chantal Supports Alzheimer's Association With Memory Collection
Wednesday July 17th, 2013 - 11:06AM
These are shortcuts to your favorite social networking and bookmark sites. Add this story to your Facebook page, del.icio.us, DiggIt, and many others!
Chantal Cookware Corp. showcased its Memory collection at the summer Atlanta International Gift & Home Furnishings Market. The new assortment, which features different shades of purple across several categories, was designed to support the Alzheimer's Association and awareness of the disease.
Incorporating cookware, bakeware, a teapot, and assorted kitchenware and personal accessories, such as mugs and medicine bowls, the program is available to retail stores this month. September is World Alzheimer's month. The company will donate 5% to 10% of Memory Collection net sales to the Alzheimer's association.
"The Alzheimer's Association has been tremendously helpful in my pursuit for information and we are thrilled to join them in the fight against this heartbreaking disease. We hope to generate needed funds for the Alzheimer's Association and to increase awareness of this disease," said Chantal CEO Heida Thurlow. "Over 20 million people in the United States are currently touched by Alzheimer's disease, either as caregivers or as patients; and, the number is growing significantly."
Tags: • Housewares • Cookware & Bakeware • Tabletop • Gadgets & Kitchen Tools •
Report: Merchandising Chief Mac Naughton Leaving Walmart »
JC Penney, Macy's Offer Best Black Friday Deals: Survey
Best Buy Remodels Chicago-Area Stores
Report: Stock Analyst Cool On Keurig Cold Potential
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When a former Navy SEAL who survived a suicide bomb attack on his barracks in Iraq talks about how to lead through perseverance, people should listen. Eric Greitens, speaking at the annual Chief Housewares Executive SuperSession, offered a compelling lesson in leadership that should resonate with any industry professional.


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"PETE ROSE: Hits and Mrs." will premiere Jan. 14 on TLC. The reality show about the Phillies legend and his former Playboy model fiancee, Kiana Kim, began shooting earlier this year.
ORLANDO, FLA. - The first time I went to Walt Disney World, I was 9 and only remember Space Mountain and Figment, the dragon from EPCOT.
Tom Hanks to mentor Melrose Park native
- TOM HANKS will help mentor aspiring screenwriter Gabriel Posey, a Melrose Park native who recently returned from serving in Afghanistan and who got hooked up with Hanks by ABC News' Bob Woodruff as part of his "Standing Up for Heroes" series.
Shallow send-off for NBC10's Lake
- VETERAN NBC10 anchor Tim Lake worked his last shift at the station Friday night, though you wouldn't have known it had you seen the 11 p.m. news.
'Hunger Games' star back in Philly for movie shoot
- LIAM HEMSWORTH and Amber Heard shot a scene at the Four Seasons on Monday for the corporate espionage thriller "Paranoia."
Bucks native to head Rock Hall of Fame
- Dan Gross: Congratulations to Bucks County native Greg Harris, who was just named the next chief executive officer of the Rock and Roll Hall of Fame, in Cleveland.
Ryan Howard weds in Hawaii
- CONGRATULATIONS TO Ryan Howard and Krystle Campbell, who were married Saturday in Maui. Not surprisingly, the Flyin' Hawaiian Shane Victorino attended the wedding, as did Ben Francisco and many of Howard's Phillies teammates.
New Philly radio station edges up the charts
- Dan Gross: Upstart talk-radio station IQ 106.9-FM had its best ratings book since its April launch during the Oct. 11 to Nov. 7 period, according to the latest Arbitron figures.
Sexy Single to put morning gig to bed
- MORNING HOST Bill Anderson , of 900 WURD-AM, is expected to announce on his Wednesday "Wake Up With Bill" show that Friday will be his last day at the station.
Bolaris' fiancee sends titillating tweets
- Dan Gross: John Bolaris' Twitter posts are often amusing, but on Sunday night, they took a different turn.
Philly stuntwoman doubles Beyonce, Rihanna
- MANY WOMEN would love to be mistaken for Beyoncé, Rihanna or Vivica A. Fox. For Angela Meryl, it's her job.
Georges Perrier: 'I'm not a bum, yet.'
- "YOU CAN TELL everybody I'm not a bum, yet. I'm fine. Don't worry," assured chef Georges Perrier when we asked about his estate sale, held over the weekend at his Chestnut Hill mansion.


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Tuesday November 25, 2014; 08:35 pm EST
Welcome the New Year at this family-oriented celebration. Start at 3pm with music, face painting and other activities for children put into play by the Annapolis Children's Museum. Be dazzled by the first fireworks display at 7:30pm. Stay for an evening of music and dancing until the countdown to the second fireworks to welcome 2014 (8pm-midnight). 3pm-midnight at Susan Campbell Park, City Dock, Annapolis: 410-263-7996.


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Romantic Restaurants in the Area near Holocaust Museum
I'm planning on visiting DC for my birthday (which is in about 2 weeks), and my boyfriend wants to check out the holocaust museum and the national museum of natural history. So we were looking for a place that is more "fine dining" than just the regular places you find everywhere that are in that area. We will be driving to DC so if it is within a 10-20 minute ride from the museums that would be fine. I've already looked into the Capitol Hill Restaurant and Art and Soul, so if you have any insights on either of those restaurants i'd like the input!
Thanks so much!!
PS...the restaurants don't have to be "romantic" i'd just like a good place where we would get good food for not a ridiculous price. anywhere between $15-30 an entree.
Art and Soul
415 New Jersey Ave. NW, Washington, DC 20001
I know it's not often mentioned on this board, I recommend Cedar in Penn Quarter (just a block away from the Archives-Navy Memorial Metro stop). It's modern American cuisine and they have a great pre-theatre dinner menu and good cocktails (not my opinion since i don't drink alcohol, but the opinion of others I know).
If you do get the pre-theatre menu, just don't order the pork dish. I remember not liking it all that much. But the butternut squash soup is excellent!
They have fairly good happy hour deals, too. Menu is on their website: http://www.cedardc.com/
Where are you staying? Why I ask is that you mention a car and going to the mall. The two generally don't work well together unless you're lucky or in the know.
That being said, I'd suggest expanding horizons for the dinner to anywhere metro accessible and/or near where you're staying.
Also - keep in mind that the mall is a big area. There are a few recent threads guiding lunch choices - you will want to plan a lunch as well to keep energies up.
Nothing says "romantic" like the holocaust museum. Seriously, if you're still in the mood to eat after visiting the museum and if you like the small plates format, I still really like Zaytinya (just follow those Penn Quarter directions).
701 9th St NW, Washington, DC 20001
re: Jason1
I second Zaytinya. (Come to think of it Oyamel is also very good -- they are both from the same chef, Jose Andres.)
Oyamel Cocina Mexicana
401 7th Street N.W., Washington, DC 20004
701 9th St NW, Washington, DC 20001
re: Jason1
Oh I know right? What a great birthday place for me! I've actually already been there, but since we were already going to go to the natural history museum and he wanted to check it out, i figured why not. He'll make up for it I'm sure;)
Art and Soul has very good food, but is a pretty boisterous place - especially when you're in the post-museum vibe. I'd recommend Bistro d'Oc on 10th between E and F. In fact, if you're comfortable walking decent distances, I''d recommend that you'd park in a garage near there and then walk (or cab, it'll only be a few bucks) between it and museums. Anyway, it's cozy and romantic, the food is delicious, and you'll be transported to Paris. Plus, it's on the same block as Ford's Theater and the house with Lincoln died, and a block north of the FBI building, so you can add those to your quick sight-seeing tour!
Art and Soul
415 New Jersey Ave. NW, Washington, DC 20001
re: katecm
I'd also suggest looking at Tabard Inn near Dupont Circle.
Tabard Inn
1739 N St NW, Washington, DC 20036
Thanks for all the suggestions!! And the tip about driving and parking. I'll look into all of them.
Proof is delicious, and Rasika is tasty too, Proof is probably a little less scene-y, but both have nice atmosphere and tasty food. :)
Rasika Restaurant
633 D Street, NW, Washington, DC 20004
I'm really surprised that no one has mentioned Sou'Wester at the Mandarin Oriental, which is within walking distance of the Holocaust Museum. It has water views, which I would consider to be romantic. I haven't been there myself, so can't comment on the food. However, it has been positively reviewed. http://www.mandarinoriental.com/washi...
re: lawhound
Besting the nominee for 2010: http://chowhound.chow.com/topics/754399
re: Dennis S
Hmm . . . missed the earlier one -- I think it's a tie. Now searching the Jerusalem board for "Haimisha restaurant near Yad Vashem."


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This rug is so soft under your feet, very thick. We were a bit nervous about ordering it, as we needed a blue red (berry), and the picture looked more orange red. It was perfect. There is some variation of red tones in the rug, so it would probably work with most red shades. Love it!!!!
I have been searching for a red shag rug for my living room for about a year...hoping to find great quality at a fair price. Overstock provided on both counts! This rug is so soft underfoot. I am so happy that I finally took the plunge and purchased. It was on sale, plus I used my Club O dollars giving me a deal I could not match anywhere. Arrived within a very few days, packaged well for shipping. Love it! Thanks Overstock!
Lovely and comfortable carpet. The color is nice bright red . I would rate it as medium plush carpet. I am using it in my basement and it's definitely added the warmth.
This rug is an excellent value! It's so plush and comfortable. I am very pleased with this purchase!
I LOVE this rug! It is soft, good quality. Great rug for the price.
I bought this red ahag rug for the living room and have been delighted by the attractive red color, the length of the shag, and most of all by the softness. Everyone just want to lie on it and walk barefoot on it. A fantastic deal!
I was a little concerned if the red would match my red poang XXXX chairs and it matches perfectly. The rug has 3 shades of red yarn which gives it depth and interest. I was also wondering how close the threads would be and if you could see the base. You can not. The threads are close and the carpet is exactly as described; cozy red shag! I am so happy with this purchase. And I can't believe the price. There is nowhere else I could find this quality for this price. Free shipping too! I'm very, very pleased with my purchase from Overstock!
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The Z Hotel, London
(+) Location. (-) Terrible sleep, noisey, dusty, uncomfortable and really shocking customer service skills (*) (On Ciao since: 02/2012)
User reviews
Travel > Europe > United Kingdom > London > London Hotels
Travel Lodge, Docklands Hotel, London
(+) Tube station 1 min walk away (-) Difficult to find (*) (On Ciao since: 12/2003)
User reviews
Langham Hotel, London
Hotel -1C Portland Place, Regent Street, London, W1B 1JA -4 Stars -429 Rooms (On Ciao since: 07/2000)
User reviews
Regent's Park, London
Park, Hotel -Address: Regents Park, Inner Circle, Outer Circle, London NW1 4NR, 156 Gloucester Place, London, NW1 6DT (On Ciao since: 07/2000)
User reviews
Travel > Europe > United Kingdom > London > London Attractions
Sanderson, London
Hotel -50 Berners St., London, W1T 3NG -4 Stars -150 Rooms (On Ciao since: 04/2006)
User reviews
The Savoy, London
Hotel -The Strand 189, London, WC2R 0EU -5 Stars -154 Rooms (On Ciao since: 12/2000)
User reviews
Ibis London Earls Court, London
Hotel -47 Lillie Road, Earls Court, London, SW6 1UD -2 Stars -501 Rooms (On Ciao since: 04/2006)
User reviews
Novotel London Tower Bridge, London
Hotel -10 Pepys Street, London, EC3N 2NR -203 Rooms (On Ciao since: 03/2004)
Ramada Encore London West, London
Hotel -4 Portal Way, London, W3 6RT, 4 Portal Way, Gypsy Corner/ A40 -3 Stars -150 Rooms (On Ciao since: 02/2006)
Hyatt Regency London -The Churchill, London
Hotel -30 Portman Square, London, England (United Kingdom) W1H 7BH -5 Stars -441 Rooms (On Ciao since: 01/2006)
Athenaeum Hotel & Apartments, London
Hotel -116 Piccadilly, London, W1J 7BJ -4 Stars -157 Rooms (On Ciao since: 06/2006)
The Halkin Hotel, London
Hotel -5 Halkin Street, Belgravia, London, SW1X 7DJ -5 Stars -41 Rooms (On Ciao since: 01/2006)
Chesterfield Mayfair, London
Hotel -35 Charles Street, London, W1J SEB -4 Stars -110 Rooms (On Ciao since: 04/2006)
The Rembrandt, London
Hotel -11 Thurloe Place, London, SW7 2RS -3 Stars -195 Rooms (On Ciao since: 04/2005)
The Landmark, London
Hotel -222 Marylebone Road, London, NW1 6JQ -5 Stars -299 Rooms (On Ciao since: 12/2000)
Novotel London City South, London
Hotel -53-61 Southwark Bridge Road, London, SE1 9HH -182 Rooms (On Ciao since: 04/2006)
Club Quarters St. Paul's, London
Hotel -16-28 Ludgate Hill, London, EC4M 7DR (On Ciao since: 03/2006)
Executive Rooms By Shaftesbury, London
Hotel -Hogarth Road, SW5 0QQ London, United Kingdom -3 Stars -24 Rooms (On Ciao since: 07/2008)
Express By Holiday Inn London City, London
Hotel -275 Old Street, London, EC1V 9LN -1 Star -224 Rooms (On Ciao since: 02/2006)
The Montague on The Gardens, London
Hotel -15 Montague Street, Bloomsbury, Covent Garden, London, WC1B 5BJ -3 Stars -104 Rooms (On Ciao since: 02/2003)
(*) Reviews by Ciao members
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Carlton Hotel London
Carlton Hotel London
CUSTOMER FAVOURITE Guests at Carlton Hotel London can start their day with an all-you-can-eat English breakfast. This London bed and breakfast is minutes away...... more
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London Shelton Hotel
London Shelton Hotel
You get low-cost refurbished rooms and continental breakfast at London Shelton Hotel. You can relax in Finsbury Park which is close to this two star London...... more
Central Hotel London
Central Hotel London
CUSTOMER FAVOURITE A friendly, family run London bed and breakfast, Central Hotel London is an excellent choice for budget travellers. Located minutes away from...... more
Hanover Hotel London
Hanover Hotel London
Hanover Hotel London is a basic London budget hotel, located close to Victoria Station and all the attractions of Chelsea and Westminster. Rooms are simply...... more
Carlton Hotel
Carlton Hotel
This traditional London bed and breakfast is located within walking distance of Victoria Underground Station, which allows you to travel around the capital with...... more
Hotel Lily
Hotel Lily
Hotel Lily is a pleasant, purpose built two star London budget hotel, located opposite the Earl's Court Exhibition Centre. Perfectly located for both business...... more
Cranbrook Hotel
Cranbrook Hotel
You can afford a quality two star hotel in London with facilities like WiFi and parking if you opt for Cranbrook Hotel in Ilford. You'll be so close to Ilford...... more
Hotel Meridiana
Hotel Meridiana
CUSTOMER FAVOURITE - Superior quality family-run hotel Tourists and business travellers keep returning to family run Hotel Meridiana. Book now to secure your...... more
Euro Hotel Harrow
Euro Hotel Harrow
This hotel offers basic London accommodation in Harrow. The nearby North Harrow Tube Station means guests can be in central London in 20 minutes. With free...... more
Acton Town Hotel
Acton Town Hotel
Whether you're looking for a well priced hotel close to Heathrow Airport or the M4, warm and homely Acton Town Hotel is the place to stay! You can book a comfy...... more
Windsor House Hotel
Windsor House Hotel
Situated in London's vibrant Earls Court, Windsor House Hotel is a small London bed and breakfast, with large affordable rooms, popular with budget travellers...... more
ABC Hyde Park Hotel
ABC Hyde Park Hotel
If you need a comfortable base for your London business trip or sightseeing stay, [[Name]] is entirely affordable. This three star hotel in London's Paddington...... more
Shaftesbury Kensington Hotel
Shaftesbury Kensington Hotel
Shaftesbury Kensington Hotel is a luxurious four star hotel in London, situated just a short walk away from the Earls Court Exhibition Centre. Perfect for...... more
Custom House Hotel
Custom House Hotel
Custom House Hotel is a three star London hotel, offering comfortable rooms complete with TV, air conditining, and wireless internet access. Located in the...... more
St Simeon Hotel
St Simeon Hotel
This money saving London hotel is ideal for guests travelling on a tight budget. Ideally located in Kensington, you'll find Kensington Gardens, Natural History...... more
Best Western Ilford Hotel
Best Western Ilford Hotel
This quality three star hotel has been refurbished to offer modern and comfortable surroundings. Relax in your well-equipped room, complete with free internet...... more
London Eye Hostel
London Eye Hostel
This Central London hostel offers a friendly sociable atmosphere with a bar, pool table and movie nights. It has a great location too, within walking distance...... more
YHA London - St Pancras
YHA London - St Pancras
If you're travelling on the Eurostar to Kings Cross St Pancras, this money saving London hostel is ideally located close by. The British Museum and British...... more
Hotel Babylon : Complete BBC Series 2 [2007] [DVD]
Hotel Babylon : Complete BBC Series 2 [2007] [DVD]
Tamzin Outhwaite and Max Beesley return in the second series of this tantalising and seductive insight into the sexy world of the of the luxury five star hotel...... more
Hip Hotels: London (HIP Hotels® Travel Format) - Thames and Hudson Ltd
Hip Hotels: London (HIP Hotels® Travel Format) - Thames and Hudson Ltd
Status: New - Offers a selection of destination hotels.
Books/Subjects/Travel & Holiday/Atlases & Maps
(*) Reviews by Ciao members
Show all results
The Tower Hotel, London
Advantages: Great location on the river Thames
Disadvantages: prices on the higher side
...I booked the Tower Hotel in London via www.lastminute.com at a price of £150 for one night room only. This was a discounted price; they normally charge around £100-£120 per standard room a night, but as this was the Olympics prices have been increased and therefore £150 was a discount price.
but bearing in mind the location of right next to tower bridge and it was the Olympics I felt it was fair...
02.09.2012 16:57 ·
Read review
Ciao members have rated this review on average very helpful
Review of The Tower A Guoman Hotel, London
Superb London Budget Hotel
Advantages: Beautiful setting in a buzzing part of London
Disadvantages: Not suitable for disabled people - no lift
...The Ashley Hotel is a superb, well-priced B&B hotel in a gorgeous location in central London. It is situated in Norfolk Square, Paddington, and is just 2 minutes walk from Paddington station.
Paddington itself is a buzzing district of London adjacent to the West End, and is packed full of shops, restaurants, bars etc. Norfolk Square is an oasis of tranquility amongst this. It is a square...
19.05.2001 15:34 (19.05.2001 15:57) ·
Read review
Ciao members have rated this review on average helpful
Review of Ashley Hotel, London
Good hotel an hour out of London
Advantages: Nice comfortable hotel
Disadvantages: A bit out of the city and a soulless location
...There?s always a compromise to be had with the cheaper end of the London hotel market; location versus size of room. In terms of room space and quality, the London Ramada Docklands was excellent. In terms of location, it felt we were stopping at an industrial park in Essex somewhere, rather than having a night in London. Still, we are rarely night owls these days so we might as well be in...
10.09.2013 18:31 ·
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Ciao members have rated this review on average very helpful
Review of Ramada Hotel and Suites London Docklands, London
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Xiao Man
Chinese Paladin 5
There are 85 cosplay photos tagged with "Xiao Man (小蛮)" of Chinese Paladin 5.
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Eurofase Admire 18 Light Foyer Pendant
Eurofase Admire 18 Light Foyer Pendant
Weights & Dimensions
|Fixture Type: Foyer pendant; Kitchen island pendant
|Style: Modern
|Shade Color: Red, white, and black
|Finish: White
|Theme: Floral
|Room Use: Entryway; Entryway; Entryway
|Bulb Type: Incandescent
|Number of Lights: 18
|Number of Tiers: 1
|Voltage: 120 Volts (V)
|Wattage per Bulb: 40 Watts (W)
|Country of Manufacture: China
About the ManufacturerEurofase carefully considers the interaction of a lighting fixture with those who enjoy it. Beyond the style or finish of a fixture is the ambiance created for the space within which it resides. Always with the goal of higher achievement top of mind, each product is inspired by human needs. With many pieces still being crafted by human hands, the authenticity of this company philosophy is carried through to the finished product.
Eurofase Admire 18 Light Foyer Pendant
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Eurofase Admire 18 Light Foyer Pendant
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Return Policy
We want you to love it. If you don't, you may return your purchase within 30 days of delivery. All you need to do is visit our Self-Service Return Center for instructions. Return credits are issued within 24 hours once the item is received and inspected. Please note that shipping charges are non-refundable. The cost of return shipping will be deducted from your refund.30-Day Return Policy
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OUR SKU # XEO1979Admire 18 Light Foyer Pendant is also sometimes listed under the following manufacturer numbers: 17536-011


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by CanuckTechNerd - 3/23/11 8:25 PM
In Reply to: BUYER BEWARE: Samsung Canada Customer Service SUCKS by 100017682
I completely agree, the customer support for Samsung Canada is horrid. They will replace nearly new products with refurbished junk. UPS charges 100$ to send my 23" monitor to Ontario, because I didn't have the original box. Then they offer a replacement that is half the price and half the quality of the original. Then it takes 2 or 3 months to receive the replacement. AVOID SAMSUNG PRODUCTS.