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News from WTOP
News from the AP
Mike Causey
Shows & Panels
- Agency of the Month
- Business of Government Hour
- The CFO Agenda: Delivering value from financial transparency and audit efforts in the public and private sector
- CXO Surveys
- Federal News Countdown
- Government Perspectives on Mobility and the Cloud
- Reducing Risk in the Cloud
- Reimagining the Next Generation of Government
Shows & Panels
- AFCEA Answers
- American Readiness: Renewable Power and Efficiency Technologies
- Ask the CIO
- Delivering the Digital Government Mission
- Federal Executive Forum
- Federal News Radio's National Cyber Security Awareness Month Special Panel Discussion
- Federal Tech Talk
- The Future of Government Data Centers
- Government Perspectives on Mobility and the Cloud
- Mitigating Insider Threats in Virtual & Cloud Environments
- Moving Agency Applications to the Cloud with PaaS
- Reducing Risk in the Cloud
- Security in the Age of Targeted Attacks
- Air Traffic Management Transformation Report
- Cloud First Report
- General Dynamics IT Enterprise Center
- Gov Cloud Minute
- Government in Technology Series
- Homeland Security Cybersecurity Market Report
- The Intersection: Where Technology Meets Transformation
- Modern Mission Critical Series
- National Cybersecurity Awareness Month
- Technology Insights
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- The Next Generation Cyber Security Experts
Shows & Panels
Roger Waldron, President, Coalition for Government Procurement.
The General Services Administration and the Office of Personnel Management will move ahead with a partnership to manage a multiple award contract for human capital and training services. The agencies issued a memorandum of understanding detailing each agency's roles -- GSA will take over facets of acquisition and OPM is responsible for services side of the contract. GSA Administrator Dan Tangherlini says the end goal is to procure these types of services at a lower price.
Jason Miller, Executive Editor, Federal News Radio
The Office of Personnel Management is teaming up with the General Services Administration to re-imagine its multi-billion dollar training contract. OPM and GSA signed an agreement yesterday to use each agency's expertise to develop the new contract. Federal News Radio Executive Editor Jason Miller told Federal Drive hosts Tom Temin and Emily Kopp about the new plans for the training and management assistance (TMA) contract. Read Federal News Radio's related article.
OPM, GSA combine forces for new take on training contract
The General Services Administration and the Office of Personnel Management sign an agreement to co-manage the new training and management assistance contract. GSA will handle the acquisition facets, and OPM will oversee requirements and services part of the contract that could be worth billions of dollars.
General Dynamics secures $17.65B contract with Navy
General Dynamics' subsidiary Electric Boat will be responsible for building 10 additional Virginia-class submarines as part of a $17.65 billion contract with the Navy.
The state of the federal market
Stan Soloway, president and CEO of the Professional Services Council, joins host Mark Amtower to discuss what the acquisition market is like for IT firms and professional services.
April 28, 2014
Jason Miller, Executive Editor, Federal News Radio
An interagency squabble over version three of the office supplies strategic sourcing contract goes public, and the General Services Administration is not happy about it. In Inside the Reporter's Notebook, Federal News Radio Executive Editor Jason Miller promises Federal Drive hosts Tom Temin and Emily Kopp a bit of humor to an otherwise dry federal procurement process. Read Federal News Radio's related article.
Inside the Reporter's Notebook: The funny side of strategic sourcing; NSTIC turns 3
In this edition of Inside the Reporter's Notebook, Executive Editor Jason Miller shares news and buzz about the IT and acquisition communities. GSA and SBA continue their ongoing quarrel over the Office Supplies 3 contract, and April marks the three-year anniversary of NSTIC's release.
Jason Miller, Executive Editor, Federal News Radio
For the first time in seven years, government leaders expect agencies to meet a major contracting goal. They awarded 23 percent of all prime contracts to small firms last year. But some in the contracting community see warning signs that signal bigger problems. Federal News Radio Executive Editor Jason Miller told Federal Drive hosts Tom Temin and Emily Kopp why some want to rain on the small business success parade. Read Federal News Radio's related article.
Pentagon sees new risks to industrial base during current spending downturn
The Defense Department and the industries it depends on have made their way through budget downturns before, but this one is different. Both budgets and threats are uncertain.
Concerns over contract bundling cast shadow over 2013 small business successes
All signs are pointing to the fact that despite the government spending less overall on acquisition last year, agencies met the statutory goal of awarding at least 23 percent of all prime contracts to small businesses. Experts say contract consolidation and bundling will negatively impact small firms in the coming years.
GSA names ombudsman to give industry a louder voice
Jeff Koses, the senior procurement executive at the General Services Administration, said the ombudsman will help vendors navigate the acquisition bureaucracy and ensure the agency is hearing and addressing challenges and concerns. It's one of several steps GSA is taking to improve vendor and agency customer satisfaction.
Budget pressures shrink federal contract spending by 11 percent
Following a year of widespread budget uncertainty, federal contract spending fell by 11 percent, according to a new analysis by Bloomberg Government. All told, agency contract spending tumbled from $516.3 billion in fiscal 2012 to $462.1 billion last year, the report found.
GSA's new contracting policy tackles IG's concerns about management interference
Federal Acquisition Service Commissioner Tom Sharpe tried to balance the need for management oversight with contracting officer autonomy. But some in industry worry that the new memo does nothing more than add more paperwork to a broken process.
Trends in Big MACs
Bloomberg Government analyst Brian Friel will talk about last year's largest multiple award contracts and trends in contract usage for big Macs.
April 22, 2014
What is the Federal Communicators Network?
David Hebert and Cori Bassett from the Federal
Communicators Network, will talk about the organization, and the services it provides to communications professionals in the federal government.
April 21, 2014
Air Force presses vendors to tackle supply chain costs
In a budget environment in which cost overruns are very likely to lead to canceled programs, the Air Force says it's pressing it prime vendors to remove any costs they possibly can from their subcontracted supplier base.
John Adams, Defense Acquisition University
The Defense Department is looking at programs to cut back or kill because of budget pressures. When you get the work to terminate your program, you don't just stop. The Defense Acquisition University's Smart Shutdown guide book tells you how to shut down the right way. John Adams, director of the specialty engineering education and training program and professor of acquisition program management and systems engineering at the Defense Acquisition University, was Francis Rose's guest on Pentagon Solutions.
The state of the national security market
David Ramirez, general manager of the Federal Solutions business unit at the L-3 National Security Solutions group, will discuss innovation and what agencies are looking for in terms of services and technologies.
April 15, 2014
Trey Hodgkins, Information Technology Industry Council
The Obama administration says discrimination is partly to blame for a pay gap between men and women. But Congressional Republicans are skeptical. They have balked at a bill to address pay inequality. In the meantime, President Barack Obama has signed an executive order for federal contractors. They will have to report to the Labor Department detailed salary information broken down by race and gender. They also won't be able to retaliate against employees who discuss salary. Trey Hodgkins, senior vice president for the public sector at the Information Technology Industry Council, told Federal Drive hosts Tom Temin and Emily Kopp how contractors are reacting to the executive order. Read Federal News Radio's related article.
What topics are trending for the federal workforce?
Julie Perkins hosts a roundtable discussion of the hottest topics in the federal government.
April 11, 2014


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iTunes Music Store now features exclusive Disney tunes
"Teaming up to offer the leading collection of digital music for kids, teens and families, Apple and Walt Disney Records today announced that the catalog of Walt Disney Records, including soundtracks from Disney's classic animated films and Disney/Pixar's computer-animated films, is now available exclusively on the iTunes Music Store through September 30th.
A new "Disney" genre page within the iTunes Music Store will feature a wide range of family entertainment including the soundtracks from animated films such as Alice in Wonderland, Beauty and the Beast, The Lion King, Snow White and The Little Mermaid; Disney/Pixar's Toy Story, Toy Story 2, A Bug's Life, Monsters, Inc. and Finding Nemo; plus, Disney audio storytellers, theatrical cast recordings and music from up-and-coming stars Raven and Jesse McCartney."
Related Stories
- Verizon sponsoring ad-free listening to all U.S. iTunes Radio users
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- Apple working to cut music subscription prices
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- U2's Bono apologizes for automatic iTunes album download
- Apple releases another iTunes 12 beta
Posted by Aladdin on April 21, 2004 at 1:57 PM (CDT)
Is it just me, or am I not seeing "Beauty and the Beast" on there, as per the press release?
Posted by bwa on April 21, 2004 at 2:35 PM (CDT)
Just kidding… i'm an idiot. It was listed under the "kids" subsection, and not with the rest of the big movie soundtracks from Disney.
Posted by bwa on April 21, 2004 at 2:37 PM (CDT)
Posted by jaypod on April 21, 2004 at 3:06 PM (CDT)
M-I-See! ...... K-E Why?
Posted by stylescraper on April 21, 2004 at 3:40 PM (CDT)
What a waste of HD space.
Posted by WOLF MAN on April 21, 2004 at 4:32 PM (CDT)
why do you ask why and why do you say its a waste? Some people like disney music, some people don't. I for one, do. I bought the aladdin, little mermaid and lion king soundtracks.
grand grand grand
Posted by j on April 21, 2004 at 4:33 PM (CDT)
This outstanding! Just like any other kind of music out there… fans of this music should be able to shop on a "song by song" basis! For parents, this is a huge plus.. you don't have to buy the whole soundtrack for the kids. Why would anyone think this is wasted space??? This is all good, and it ain't hurting anyone.
Posted by ntljr in Woodland Hills, CA on April 21, 2004 at 6:14 PM (CDT)
All music added to iTunes is good. As much as possable, except William Hung. ;) j/k
Posted by Joe Matt on April 21, 2004 at 7:05 PM (CDT)
Interesting, Disney and Steve Jobs trying to mend old rifts?
Posted by Eisner on April 21, 2004 at 7:05 PM (CDT)
I am getting a bit tired of hearing about itms - still not available in Australia some 4-6 months after originally scheduled. Other music stores can operate in this country but it seems Apple don't care about markets outside USA.
Posted by Neil on April 22, 2004 at 5:37 AM (CDT)
Waste of HD Space? Come on! Who doesn't like Louis Prima's "I wanna be like you" from Jungle Book? Or The Nightmare Before Christmas soundtrack? Or the original "Cruella De Vil"? You guys need to be more open to some of the classic Disney fair before it went to the toilet.
Posted by ikelleigh on April 22, 2004 at 12:07 PM (CDT)
Neil….I feel your pain. Not having iTunes available in other countries is extremely frustrating. Will it ever happen? Also….what is the deal with Steve Jobs and Disney…."mend old rifts"...what happened?
Posted by jaypod on April 23, 2004 at 2:28 AM (CDT)
Disney is great, so is their music. What's the Big problem with it being offered on the ITMS. Infrances on, music store.
Posted by Hidden Dragon on April 23, 2004 at 4:15 PM (CDT)
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subset: commoncrawl


text:
WILKES-BARRE — The painstaking work of demolishing half of the condemned Engle Building while protecting a next door dress store partially connected to the property began Wednesday.
Most of the demolition will be done by hand, said Liza Prokop, spokeswoman for the city.
"They're taking as much care on that as they can," Prokop said.
Stell Enterprises Inc. of Plains Township was awarded the $194,861 emergency demolition contract last week to tear down four condemned buildings, three of them owned by the city the other by the Wilkes-Barre Redevelopment Authority, that had deteriorated to the point of being in danger of collapse near the intersection of South Main and West Northampton streets. Two buildings in the middle of the cluster are to be spared, complicating the demolition process that began Monday. The work is expected to last until at least Nov. 22.
Workers razed two city-owned buildings at 73 and 75 S. Main St. Nearly half of the roof and most of the rear wall of the city-owned property at 71 S. Main St. were knocked down, exposing the collapsed floors. The brick side walls remained intact and one of them stood approximately eight feet from the temporarily closed Place One at the Hollywood at 67 S. Main St.
The demolition plan calls for leaving the front of 71 S. Main standing that's connected to the dress store by a 40-foot section of wall. The buildings also share a facade dating back to the 1800s.
The dress store and the Frank Clark Jeweler next door at 63 S. Main will stay if all goes according to plan. The stores were determined to be structurally sound by an engineer hired by the city to inspect its properties being shopped to a potential developer. But the conditions of the neighboring buildings put them at risk of damage and the city ordered them to temporarily shut down.
The city paid to relocate the contents and furnishings of Place One to Scranton to a property the store's owner Michaelene Coffee closed in order to move to Wilkes-Barre. The city also will pay to return the items to Wilkes-Barre. The total cost of the trips is $8,800. The owner of the jewelry store, Ilona Bruns, relocated to her other jewelry store Ocean Gold store in Nanticoke. Her property is next 61 S. Main St. the Redevelopment Authority's building targeted for demolition.


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Oilers/Titans' top plays: Music City Miracle
July, 8, 2014
Jul 8
By Paul Kuharsky | ESPN.com
Allen Kee/Getty Images» VOTE HERE » NFC Plays: East | West | North | South » AFC: East | West | North | South
This is one of three nominations for the most memorable play in team history. We've already looked at Mike Renfro's non-catch catch in the 1979 AFC Championship Game. We will also feature Kevin Dyson's desperate, unsuccessful reach for the end zone that came up a yard short of forcing overtime as time expired on Super Bowl XXXIV. Please vote for your choice as the Oilers/Titans' most memorable play.
Score: Titans 22, Bills 16
Date: January 8, 2000 Site: Adelphia Coliseum
As a head coach, Jeff Fisher prided himself on having his team ready for everything.
In a playoffs-or-pink-slips season, the franchise's first as the reinvented Tennessee Titans, Fisher got to offer the best possible example of that.
The Titans responded with "Home Run Throwback," a play designed by special teams coach Alan Lowry. It called for Frank Wycheck to field an expected squib kick, sell the coverage team he was returning it to the right, and then whirling and throwing a lateral to Derrick Mason.
Mason was out with a concussion, however, and his backup for the play, safety Anthony Dorsett, was also unable to play.
Kevin Dyson was quickly coached up to take on the role.
It wasn't Wycheck, but Lorenzo Neal who fielded the kick. He handed it back to Wycheck, who sold the fake and made the throw. Dyson came back to collect the low throw, turned and sprinted into wide-open space with a convoy of blockers ahead of him. He pulled up and eased into the end zone for a 75-yard touchdown.
It withstood a replay review by referee Phil Luckett, and provided the winning margin for a team that went on to make the franchise's lone Super Bowl appearance.
The Music City Miracle added to the lore of Buffalo sports teams coming up short, while creating an incredible story in Tennessee in just the ninth meaningful game played in the stadium built to draw the Oilers from Houston to Nashville.
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Job for a Cowboy
'Sun Eater' is the fourth full-length album from Job for a Cowboy, and was released last week. The response has been overwhelmingly positive and has led to a #91 debut on the Billboard Top 200 Chart!Read More
King Diamond
King Diamond's 'Dreams of Horror' was released last week and has debuted at #28 on the Billboard Hard Music charts!Read More
Between the Buried and Me
Between the Buried and Me has confirmed two headlining shows in Mexico for next year. The band will be heading to Guadalajara and Mexico City on the 20th and 21st, respectively.Read More
Thomas Giles
Thomas Giles is set to released 'Modern Noise' on November 25th, 2014. Today, AbsolutePunk.net has been tapped to debut a different sounding cut from the album. The electronically-driven 'We Wander Lonely' is streaming now.Read More
RAM / Portrait
Swedish heavy metal titans RAM and PORTRAIT join forces for a split album entitled 'Under Command' to be released the 8th of December! PORTRAIT have now released their cover of RAM's 'Blessed To Be Cursed'!Read More
Thomas Giles
Thomas Giles, the solo project from Between the Buried and Me front man, Tommy Rogers, is set to released 'Modern Noise' on November 25th, 2014. Today, All Music is set to debut a brand new music video from the album!Read More
Rivers of Nihil
On the eve of Rivers of Nihil's upcoming tour with Death (DTA Tours), the Reading, PA based metal act have launched a brand new video, 'Place of Serpents'.Read More
New Releases
Metal Blade TV
Upcoming Releases
Latest News
Tommy Rogers to release new Thomas Giles album next month; new track streaming now
- October 6th, 2014 – Between the Buried and Me vocalist/keyboardist...Read More
Cripper launch new track and unveil the cover art for "Hyëna"
- October 2nd, 2014 – Hannover, Germany's Cripper have debuted...Read More
The Black Dahlia Murder and Suicide Silence co-headline tour this October!
- October 2nd, 2014 – Mark your calendars! The Black Dahlia Murder has...Read More
REVOCATION Post Third Video from the Making of "Deathless"
- October 1st, 2014 – Metal Blade Records Debut Out October...Read More
PRIMORDIAL to Release New Full-Length this November via Metal Blade Records
- September 30th, 2014 – Lyric Video Now Playing + Pre-order Packages...Read More
Ensiferum nearing completion on recording new album!
- September 29th, 2014 – Ensiferum have been locked away in Astia...Read More
In Solitude release "Sister" on white vinyl and cassette
- September 29th, 2014 – European tour with Beastmilk begins next...Read More
Nonpoint debut "Breaking Skin" video!
- September 29th, 2014 – Nonpoint have released their eighth studio...Read More


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|CIB Description — BanColombia, S.A.
Bancolombia is an independent financial institution, providing a range of financial products and services to a diversified individual and corporate customer base throughout Colombia. Co.'s businesses comprise the following segments: Retail Banking, Commercial Banking, Small Business Banking, Construction Banking, Government and Institutional Banking, Treasury, Off-shore Commercial Banking, Trust, Leasing, Brokerage, investment Banking, Manufacturing among others. As of Dec 31 2012, Co.'s total assets and total deposits amounted to Col$97,916,380,000,000 and Col$64,158,720,000,000, respectively. Also as of Dec 31 2012, Co. operated 993 branch offices, throughout Colombia.
Fri, Sep 19, 12:00 PM, StreetAuthority
Looking at a recent chart of share prices, you& 039;d think shares of some Latin American companies were on fire and might be tempted to jump in for double-digit gains.
Shares of
Tue, Apr 22, 7:30 AM, Zacks


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Unfortunately BBC Shop does not ship to your country. Please try Amazon for more great Doctor Who products.
Doctor Who: The Web of Fear has become the biggest selling classic Doctor Who DVD in the 15 year history of the range.
James Robert McCrimmon was a piper and soldier, fighting the hated English at Culloden in 1746. Escaping execution, Jamie follows Polly, Ben and the Doctor into the TARDIS. Although from the 18th Century, Jamie adapted quickly to time-travelling life, fighting Cybermen on the Moon, faceless Chameleons and Daleks in Victorian London. He faced the Great Intelligence – once in Tibet and again in the London Underground – and twice fought the Martian Ice Warriors. What he lacked in intelligence, he more than made up for in strength, charm and instinct. With his memories of the Doctor erased, he was returned to Scotland on the day he left by the Time Lords at the Doctor's trial.
I tell you I'm a Highlander, the cold doesn't affect me.
Unfortunately BBC Shop does not ship to your country. Please try Amazon for more great Doctor Who products.
As Jamie and the Doctor look on in horror, the Cyber Controller revives...
Jamie actor Fraser Hines talks about impersonating the Second Doctor
Zoe and Jamie are left spinning in the vortex when the TARDIS explodes
The Doctor calls on the only people who can help him – his own terrifying race…


text:
FlorenceAdd to Wishlist
Florence Artisan Album Plus, Multi-ring 11x14" with 10 Super Clear Archival Page Protectors, Black
SKU: FLOFAA0314 MFR: FAA0314Please Note ! This item will be Drop-shipped to you directly from Manufacture/Distributer. Prompt Delivery is subject to availability. This item is not returnable
What's Included
What's Included With This Item:
- Album
- 10 Super Clear Archival Pages
Ways to Save
Why Adorama?Easy Returns, Fast Shipping, Trusted for over 35 years.
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text:
At the WWE NXT tapings tonight from Full Sail University, Big E Langston defeated Seth Rollins to win the NXT championship after hitting the Big End. The Shield interfered but the majority of the NXT roster chased them away and Big E kicked out of Seth Rollins' Shiranui attempt. Langston countered a second one into the Big End.
After the match the roster celebrated with Langston as Triple H and Dusty Rhodes came out and raised his hands up. Posted below is a photo of Langston celebrating.
We should have full results up shortly.
The Hottest WWE news, photos and videos delivered to your inbox in one convenient newsletter. Sign up now and give it a try!
|WWE Survivor Series Report - 11/23/14
|WWE RAW Report - 11/24/14
|Sting Makes His WWE TV Debut, Possible Match Against Triple H?, Update on The Authority
|Backstage News on Sting vs. Triple H or The Undertaker, Speculation on Sting's WWE Future
|Backstage News on TNA's Talks with Spike, What Josh Mathews Is Working On, TNA Live Events


text:
Important Announcements
Christmas sale is up! Read for detailsAfter seeing people's responses I've decided I'd open the sale. Starting it at a decently early time would allow me plenty of time to get any christmas comms done before the season passes.Christmas sale is up! Read for details by HelixJack
500,000 views!A little over 7 years ago I first joined the site, my first submission being a crappy little pencil drawing. I remember getting a good response from a few people both here and on the AVGDID board, but I had no idea that I would still be here 7 years later, still making DID stuff (but mostly lurking! ).500,000 views! by sikstiks
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I Was Attacked by a Gang of Old People at a Protest in Bucharest
First they surrounded me, and then they started booing. They said that I am an agitator, an instigator, and that I have been brainwashed. Then they pulled me by my bra and told me to "Get the fuck out of here, you sleazy cunt."


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Echo Chamber: Solange Is Not a "Vocal Muse"
By Jenn Pelly on April 25, 2013 at 1:00 p.m. EDT
Photo by Ebru Yildiz
"I find it very disappointing when I am presented as the 'face' of my music, or a 'vocal muse' when I write or co-write every fucking song. How can one be a 'vocal muse' to their own melodies, story telling, and words they wrote?"
-- Solange defends the significant artistic contributions she makes to her own records. (Via Twitter)
Echo Chamber: Grimes Fights Back
By Jenn Pelly on April 24, 2013 at 10:17 a.m. EDT
Photo by Chris Tuite
"I don't want to have to compromise my morals in order to make a living. I don't want my words to be taken out of context. I don't want to be infantilized because I refuse to be sexualized. I don't want to be molested at shows or on the street by people who perceive me as an object that exists for their personal satisfaction. I don't want to live in a world where I'm gonna have to start employing body guards because this kind of behavior is so commonplace and accepted and I'm pissed that when I express concern over my own safety it's often ignored until people see firsthand what happens and then they apologize for not taking me seriously after the fact…"
Echo Chamber: Morrissey on the Late Margaret Thatcher
By Carrie Battan on April 8, 2013 at 4:55 p.m. EDT
"Thatcher will only be fondly remembered by sentimentalists who did not suffer under her leadership, but the majority of British working people have forgotten her already, and the people of Argentina will be celebrating her death. As a matter of recorded fact, Thatcher was a terror without an atom of humanity."
-- Morrissey will not remember the late Margaret Thatcher fondly. (via Daily Beast)
Echo Chamber: James Blake on Downloading His New Album Illegally
By Evan Minsker on April 7, 2013 at 11:17 a.m. EDT
"And why wouldn't you [download Overgrown illegally]? My label [Universal] is hoping that on April 8 you'll do the right thing and click the 'Buy' button. You should see what they're doing online just to get people to look at the 'Buy' button. I'm starting not to care, to be honest. Things are changing. The ship isn't just going down. There are people trapped inside, bashing on the windows trying to get out."
-- James Blake is not happy with his label's publicity tactics, including that they shared five of the album's 10 songs early. [via The Guardian]
Echo Chamber: Nicki Minaj
By Carrie Battan on April 3, 2013 at 11:20 a.m. EDT
"I am lyrically better than most of the male rappers out there. Yes, I'm going to say it. I don't get the credit that I deserve... I've put in my work lyrically, and people act like it doesn't exist."
-- Nicki Minaj takes on sexism in the music industry. (via MTV)
Echo Chamber: Andrew W.K. Wins April Fools' Day
By Jenn Pelly on April 1, 2013 at 12:41 p.m. EDT
Photo by Danny Pardoe
"As of today, I've officially decided to never party again."
-- Pack up, head home. April Fools' Day is over because Andrew W.K. just won. (via Twitter)
Echo Chamber: Iggy Pop on Iceage
By Evan Minsker on March 24, 2013 at 8:07 p.m. EDT
Photo by Colin Kerrigan
"It's not easy to be that dark. A lot of people who try to express negative energy sort of just flail. They kind of come off like hamsters or something, where the more they try, the sillier it is. I thought they achieve a certain darkness. … They're known for doing very, very short albums and shows. When I started, that's what we always did."
-- Iggy Pop shares his appreciation for Iceage on Australian radio show "triple j Mornings", where he played "You're Nothing" on a playlist alongside Chuck Berry, Kraftwerk, Jacuzzi Boys, and Peaches. Earlier in the show, he called them "the only current punk band I can think of that sounds really dangerous". (via Maria Sherman)
Echo Chamber: Steve Albini on Jason Molina
By Jenn Pelly on March 19, 2013 at 10:30 a.m. EDT
Photo by Steve Gullick
"I loved hearing Jason Molina sing. He was a genius at turning a phrase and making it into something more than the words in it. Jason was almost supernaturally prolific, and several times I watched him write an album's worth of songs in a weekend, recording them on the spot. Much of his recorded output with Magnolia Electric Co is the evidence of him and the band playing his songs for the very first time. It's amazing, really, that it was any good at all, much less so touching and fully realized. Jason was a unique talent and I will miss him. My heart goes out to all his friends and family, all of you I've met have been good people who did well by Jason."
-- Steve Albini, who worked on Molina's Songs: Ohia record Magnolia Electric Co. and several albums under the Magnolia Electric Co. moniker, has posted a tribute to the late singer-songwriter, who passed away Saturday, March 16 at 39, on the Electrical Audio message board.
Echo Chamber: DIIV: "Fuck SXSW"
By Carrie Battan on March 14, 2013 at 2:04 p.m. EDT
Photo by Erez Avissar
"Hi Austin. Fuck SXSW. There… I said it.
Here, the music comes last. 5 minute set-up, no sound check, 15 minute set. The 'music' element is all a front, it's the first thing to be compromised. Corporate money everywhere but in the hands of the artists, at what is really just a glorified corporate networking party. Drunk corporate goons and other industry vampires and cocaine. Everyone is drunk, being cool. 'Official' bureaucracy and all their mindless rules. Branding, branding, branding. It's bullshit… sorry."
-- DIIV's Zachary Cole Smith is not enjoying his time in Austin. (via Tumblr)
Echo Chamber: Trent Reznor Gives Pretty Much the Best Reddit AMA Answer of All Time
By Amy Phillips on March 5, 2013 at 4:35 p.m. EST
Photo by Rob Sheridan
"Sorry, the wifi on our yacht is having issues, we can't get your full question to load. Try sending me an email at firstname.lastname@example.com"
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Site Management Software
Showing page 2 of 2.
It's an administration space for your web site, and you can view stats, do pings and much more. You need a MySql database1 weekly downloads
The aim of this project is to introduce all code used to power powlow.com to the public domain, with releases under the GPL. Coding is in the Hypertext Preprocessor Scripting Language PHP, with MySQL for database needs.1 weekly downloads
Adisis Drupal Framework for Delphi
Adisis Drupal Framework for Delphi (aka AdisisDrupal) is a collection of classes to administer Drupal sites.0 weekly downloads
GesPat is an Open Source content managament system (web), full oriented to work on campany enviroment and with a personal use to. GesPat can be easy translated to many lenguage easy and include full suport from jabber protocol and google api power.0 weekly downloads
MySHOP CMS
MySHOP Content Management System Project. Downloads. DesarrolloNukeET.org website. Spanish and English.0 weekly downloads
Seed is a ZendFramework-based comprehensive and mainly PHP-based API to assist developing web-based applications on a solid space of modern features such as DBMS abstraction, AJAX and XML.0 weekly downloads
Vlog is a little collection of php scripts which can be used as a web diary. Its purposes are simplicity in two ways: simple code and simple interface. Vlog does not really handle users, cookies, sessions etc, it just worries about the web content.0 weekly downloads
Webins en Catala
Potent i versatil administrador de fitxers remots traduit al catala programat amb PHP. Permet carrega i descarrega d'arxius ,edicio ,navegacio per directoris ...0 weekly downloads
Xalana Community System
Xalana Community System is a framework to manage multiple communities at once. The system helps the creating and managing multiple communities with one single installation. Communities can share things with a central site or with the other communities.0 weekly downloads
XoopsCAT it is the first fully Catalan CMS based on Xoops and Catalan modules. It is also the Catalan Support Site of Xoops.0 weekly downloads
eBlog is an easy to use blogging system. It has an powerfull modules-extensions system that allows you to extend and customize it. || PROJECT ABANDONED ||0 weekly downloads
Programa per linux per baixar-se links de megaupload automàticament sense estar-ne registrat. Pot passar-li com a parametre un arxiu amb un llistat de links de megaupload. Resol el captcha i es baixa l'arxiu.0 weekly downloads
phpMiX Open Source eXperiments
The phpMiX project, here at SF.net, hosts a collection of several "eXperiments" related to other Open Source packages such as phpBB, Drupal, etc. Feel free to visit http://www.phpmix.org for further information.0 weekly downloads
phpwebeasy It's a set of phpscripts aimed to make easy the administration of a personal web page.0 weekly downloads
<workware/> is virtual appliance with a suite of turn-key applications ready built and configured for business. It is composed of the best in open source software; configured and tailored to the needs of SOHO/SMB businesses and distributed workforces.0 weekly downloads
xinu stats - webmasters dashboard
xinu is web tool for quickly check stats about website0 weekly downloads


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... CA), buy cars or find car dealerships in California(CA) area from the largest auto dealer directory ... Legacy Auto Sale # 4174 ... North State Auto Sales # 5133 19995 ...Site Report
Business listing for Marros Fancy Dog Ranch in Oroville, CA. 41 Shady Oak Dr. (530 ... Real EstateSite Report
28 Heald College Jobs available in Concord, CA on Indeed.com. one search. all jobs.Site Report
Tires in Stockton, CA on Yahoo! Local Get Ratings amp; Reviews on Tires with Photos, Maps, Driving Directions and more.Site Report
Find dogs for sale, cats for sale, guinea pigs for sale, fish and more. Buy and sell pets in Isle of Man today with free pet ads.Site Report
Learn information about Jamke Rentals, a Supplier in Modesto, CA. MacRaesBlueBook.com has the latest phone number amp; address for this business amp; others like it.Site Report
Fancy Town in Torrance, CA -- Map, Phone Number, Reviews, Photos and Video Profile for Torrance Fancy Town. Fancy Town appears in: Gift ShopsSite Report
Cakes in fremont, ca Art Deco Treasure Teddy Bear Soccer Cinderella Cake for a Birthday party Pillow cake with glass shoe slipper Tinkerbell Birthday Cake Dora ...Site Report
Game with miniature oil well rig in a transparent dome ... source of electrical energy 12 to operate the pump 4 to continuously pump oil up ...Site Report
auto parts for Napa, CA. Find phone numbers, addresses, maps, driving directions and reviews for auto parts in Napa, CA.Site Report
Dr. Parul Gupta MD company profile in Tulare, CA. Our free company profile report for Dr. Parul Gupta MD includes business information such as contact, sales and ...Site Report
Kovim, Inc. has a location in Fremont, CA. Active officers include Parul Gupta. Kovim, Inc. filed as a Articles of Incorporation on Friday, June 02, 2006 in the state ...Site Report
Get your car checked by a mechanic at Escondido Transmission in Escondido, CA 92029, with shop ... 1175 Industrial Ave Ste O Escondido, CA 92029 (1431)Site Report
How to Replace a Low Oil Pressure Switch in a Ford Taurus. A low oil pressure switch is used for a light on the ... oil is used to cool the air in an air conditioner ...Site Report


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I am pleased to own this cookbook and to be reminded of the wonderful meais I enjoyed in this very fine establishment. Read morePublished 14 months ago by VHP Very Honest Person
I bought this for my grandson since he is a chef. He told me he is very pleased with it and loves it.Published 20 months ago by Choctaw Jane
Chanterelle was a great restaurant and this book provides a wonderful walk down memory lane as well as many amazing recipes.Published 22 months ago by James Lee
While the book is a beautiful, colorful, and exquisite creation, it is a joke for home cooks. Here are the primary ingredients in the first 150 pages-- caviar, quail eggs, foie... Read morePublished 24 months ago by Freddybob
This is a beautiful book with pics and text that will inspire the serious cook. It is not as practical as his "Staff Meals" book, that I use very often. Read morePublished on November 12, 2011 by ric
AMAZING book!!! By just reading the story in the beginning I could tell this was going to be an fantastic book. Read morePublished on July 13, 2010 by Nate!!


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The 1M coupe is said to be coming in only three colors – Valencia Orange, Black, and Alpine White, the latter of which is previewed here by our Alpine325ci. Seen here is a rear rendering which follows the stunning front rendering from a few weeks back.
BMW may be uncovering the 1 Series M Coupe slowly, but Alpin325ci has accelerated the process with his amazing renders. His latest reveals what the 1M rear will likely resemble, based on the latest revealed part on the actual car.
Alpine325ci gives us a digital preview of what the 2012 M6 coupe could resemble in yet another stunning image. The 2012 M6 coupe will share the same motor and drivetrain as the upcoming M5 will debut at the Geneva Motor Show in March 2012.The M6 Cabrio will follow later in 2012. M6 Gran Coupe will arrive in late 2013. A Possible M6 Shooting brake is on the cards.
New BMW 1M info and images have slowed, but Gerald Alvarez's renditions of the upcoming 1 series M have not. Here is his latest impression of the future BMW hot coupe.
It may be a while until we see the 6-series coupe in various colors, but thanks to the talents of philip, here is a preview of the beautifu new coupe in some likely production colors.
This time around, Gerald (Alpine325ci) unleashes the white F10 M5 through his skills and imagination. So which one do our members prefer – the white or black F10 M5? We'd take either!
Gerald unleashes the production F10 M5 in his latest work. Most of what you see here should be very accurate as they are based directly on the latest revealing F10 M5 spy photos. The render quality is again top notch, especially the wheels, which look spot on to the wheels in the M5 spy photos.
Gerald is exhibiting his artistry once again with this 1M Coupe in Alpine White render. We hear he is working on a black 1M Coupe next. At this rate, we'll have previews of the 1M Coupe in every color prior to their actual unveils
Alpine325ci is at it again, this time producing an absolutely gorgeous wallpaper render of the upcoming 1M Coupe, set inside a tunnel at high speed. The 1M in the image looks spot on based on all that we've seen of the 1M Coupe so far!
Autocar.uk has published a rendering of the upcoming Mini Sport Activity Coupe (or what some have dubbed the Countryman Coupe or Mini X6).
According to Scott26, "Such a vehicle is being worked on which will be seen soon as a Concept."
The idea for the Mini Sporty Activity Coupe comes in response to Land Rover's Range Rover Evoque, with its two-door coupe styling and configuration. According to Autocar, if the Mini SAC makes production, it would hit the market around 2013, in a 4 door layout (rear doors … CONTINUED
Our resident photoshop wizard, Gerald Alvarez, has again produced some great renders to share with the Bimmerpost community. These are of the upcoming1M Coupe – front and rear view. Check out the stunning renders inside!
The cover of the latest auto moto, a french auto magazine, features their prediction of what the 2012 F30 3-series sedan may look like. This render seems to draw heavily on cues from the newest BMWs such as the F10 5-series, F07 5-series GT, F01 7-series, and even E89 Z4. Opinions?


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It is sad to see people treated as a joke when they are trying to be serious, but on the other hand, thanks for the laughs.
In response to supposed legal restrictions on the interrogation of U.S. citizen and suspected Times Square SUV bomber Faisal Shahzad, Senator Lieberma...
The United States Constitution is the charter document of democracy in America. It is what makes us who we are, and we go outside the boundaries of that charter at our peril.
When spending $3 trillion dollars, invading two Muslim countries and imprisoning kidnapped innocents in black sites isn't enough, what can the Liebermans, McCains, Giulianis and Pete Kings say?
The consequences of not reading rights to terrorist suspects that we later want to prosecute are now on display at the military commissions in Guantanamo Bay, Cuba. And it's not looking good for the government.
While some Republican politicians attempted to turn the Shahzad chase and investigation into an occasion for attacking civil liberties, it is clear that it moved forward in an extremely effective and expeditious manner.
United States action on global warming is long overdue. But energy industry lobbyists and their Senate allies have thwarted the best efforts of three ...
TWITTER: @GreenNewsReport The 'GNR' is also now available on your cell phone via Stitcher Radio's mobile app! IN TODAY'S RADIO REPORT: More on L...
With Crist still in the Senate race as an independent candidate come election day, nothing will stop Marco Rubio from taking over.
Senator Kerry and Senator Lieberman are telling everybody who has a microphone that their climate bill isn't dead. But their aggressive reassurances are misplaced energy.
TWITTER: @GreenNewsReport The 'GNR' is also now available on your cell phone via Stitcher Radio's mobile app! IN TODAY'S RADIO REPORT: Rememberin...
Senator Graham has threatened to pull his support for the bipartisan climate bill due to his frustration over immigration reform taking priority on the Senate calendar.
I was saddened to hear the details of the climate bill soon to be released by Senators Kerry, Graham, and Lieberman. This bill could be a step backwards unless the Senators fix key provisions in the upcoming weeks.
The latest disappointing news is that Kerry plans to drop the tax on transportation fuels.
In principle, a new federal climate change policy could allow states to opt out if they implement a program at least as stringent. But why should states want to opt out?
The Senate has an opportunity to ensure that U.S. troops in Afghanistan -- already contending with heroin-financed Taliban attacks -- don't have to also worry about illegal logging as well.


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When a character is primarily a Nerd (or possibly Geek), but still manages to pull off feats of Action Heroism, then they are a Nerd Action Hero. Sometimes this is the result of Character Development, where the character starts out as a Nerd but over time develops heroic skills. In other cases the character is a Hidden Badass. Sometimes a Nerd Action Hero may be paired with an Action Girl, and aforementioned Character Development may occur as a result of their interaction. There is an overlap with Badass Bookworm, but many of those are Badass first and bookworm only as a bonus. There can also be an overlap with Science Hero, but a Nerd Action Hero tends to do more ordinary Action Hero things (shooting, fighting, jumping, etc.) when the action starts. For characters who generally take Guile Hero or The Smart Guy roles to the point where viewers might even forget they're devastatingly effective at getting physical, they might have Minored In Ass Kicking.
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One of the many pitfalls for Barack Obama and his handlers is the tendency to be too cute by half -- think of that cotton candy Greek temple erected on Invesco Field in Denver for the winning candidate's acceptance speech last summer. The Washington Post's Dana Milbank makes a good case today that t ... More >>
New York turns 40
[Our weekly feature on conservative blogs' recent offenses to reason; archive here.] Barack Obama's acceptance speech at INVESCO Field in Denver was watched by a record-breaking 38.4 million television viewers. Between them and the 80,000 people who were on the scene, the chances of spinning his st ... More >>
Obama's nomination and upcoming speech at INVESCO Field in Denver are such big news that even New Yorkers are noticing. Gowanus Lounge reproduces a couple of Obama wall images in Brooklyn. Williamsburg is Dead sends its readers to tonight's "Baracktail Party" at Pete's Candy Store: "bring your appet ... More >>


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South Beach Tow is an American television series that portrays dramatized reenactments of the day-to-day business of Tremont Towing. Although the program is fictionalized, Tremont Towing is a real Miami towing company. The series premiered on July 20, 2011, on truTV. The first part of Season 2 premiered on September 19, 2012, and returned after hiatus on May 15, 2013. Season 3 premiered on October 30, 2013 and ended on June 25, 2014. Season 4 premiered on September 10, 2014.
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This invention comprises of construction of a recombinant CHO cell line that produces functional mannose binding lectin (MBL) and of special uses of MBL. Specially, use of MBL for development of a therapeutic agent for patients having systemic infection with viruses, bacteria, or fungus is described. More specifically, MBL as a trigger for complement activation and MBL so formulated to activate complement system for the purpose of treating patients with microbial infection.
1. A transformed host cell which comprises a DNA construct comprising a cloning vector which comprises:
a) a sequence of a human mannose-binding lectin (MBL) coding region, said sequence being sufficient to express a recombinant MBL in said transformed host cell,
b) an SV40 promoter DNA sequence operatively linked to said sequence of human MBL coding region,
c) a sequence of nuclear matrix attachment region element of beta-globin gene sequence,
d) an SV40 poly A, and
e) a transcription termination sequence of a gastrin gene,
said recombinant MBL exhibiting specific binding to pre S portion of Hepatitis B virus envelope protein and complement activation in the presence of MBL-binding glycosylated protein and serine proteases, wherein said transformed host cell is deposited to the International Depository Authority under the accession number KCTC 10472BP.
2. A method for producing recombinant MBL, said method comprising the steps of:
(a) providing a transformed host cell which comprises a DNA construct comprising a cloning vector which comprises:
a sequence of a human MBL coding region, said sequence being sufficient to express a recombinant MBL in said transformed host cell,
an SV40 promoter DNA sequence operatively linked to said sequence of human MBL coding region,
a sequence of nuclear matrix attachment region element of beta-globin gene sequence,
an SV40 poly A, and
a transcription termination sequence of a gastrin gene,
said recombinant MBL exhibiting specific binding to pre S portion of Hepatitis B virus envelope protein and complement activation in the presence of MBL-binding glycosylated protein and serine proteases;
(b) expressing in a cell culture system said sequence of human MBL coding region to produce recombinant MBL; and
(c) purifying the recombinant MBL produced in step (b);
wherein said transformed host cell is deposited to the International Depository Authority under the accession number KCTC 10472BP.
This application claims priority under 35 U.S.C. Section 119 to Korean Patent Applications 2003-0037511, filed Jun. 11, 2003; and 2003-0041051, filed Jun. 24, 2003.
The present invention is directed to recombinant human mannose-binding lectin (rhMBL), vectors and transformants for the production of recombinant MBL (rhMBL), use of rhMBL for prophylaxis and treatment of infection and/or immunedeficiency.
MBL is a serum protein involved in innate immunity. The molecular weight is 32 KD, consisting of carboxy-terminal carbohydrate binding domain (CRD), collagen domain, and amino-terminal cysteine-rich region. The collagen domains of three MBL molecules form a triple helix resulting in a formation of a trimer, and then up to six units of the trimers form a giant molecule by inter molecular disulfide bonding using amino-terminal cysteines.
The MBL is associated with other proteins such as MBL associated serine proteases (MASP 1, MASP 2, or MASP 3) or MBL associated protein (Map19). Therefore, the overall molecular shape of MBL is similar to the first component of complement system (C1q). The function of activated MBL is also similar to C1q, but unlike C1q it activates complement system by cleaving C4 and C2. Activation of MBL requires binding of microorganisms with unique glycosylation pattern on their surface proteins. In this process the MBL associated serine protease is activated, and C4 and C2 are cleaved in a similar way that C1q associated serine proteases (C1r and C1s) triggers activation of complement system by cleaving C3. MBL binding to microorganism also prepares the microorganism for efficient phagocytosis, as if it is an opsonins.
Previously MBL gene has been expressed in various cell lines, including CHO cell (Katsuki Ohtani et al, J. of Inmunol. Methods 222, 135�144, 1999) or HLF hepatoma cell line or HEK 293 EBNA cell (T. Vorup-Jensen et al, International Immunopharmacology 1, 677�687, 2001).
High yield expression was possible in a CHO cell, but the MBL recovered was mainly monomers and dimers without significant amount of oligomers. Expression of MBL gene in transformed human cell lines produced significantly more oligomers, but the over all production yield was less than 1 ug/mL culture media.
It is well known that only high molecular weight form of MBL complex is capable of activating complement system. Activation of complement system is very important in defense against microbial infection. It not only prepares for invading microorganism for efficient phagocytosis and direct lysis, but also it helps efficient induction of adaptive immunity.
Serum level of MBL varies widely in different individuals, ranging from 50 ng/mL or lower to over 3 ug/mL serum mainly due to genetic variation. Genetic variation includes point mutations on exons and mutations on promoter regions. Generally individuals with low level of MBL are susceptible from microbial infection. Specially, those newborn children with low level of MBL are dangerously susceptible from infections. According to one survey (Y. Hakozaki et al, Liver, 22, 29�34, 2002), the mortality of patients going through hepatic failure due to Hepatitis B virus infection depended on the serum level of MBL. Patients with high level of MBL (3 ug/mL) did not die, whereas 80% of the patients with low MBL level died. Therefore those individuals with low MBL level might benefit from MBL supplement.
Therapeutic use of recombinant MBL requires not only high level expression of MBL for industrial quantity production but also high molecular oligomeric form of MBL.
We have successfully established recombinant CHO cell line that produces not only high amount of MBL (100 ug/mL culture media) but also significant amount of oligomeric form of MBL.
This invention provides a recombinant CHO cell line producing high molecular weight, oligomeric form of rhMBL for a large scale production to be used for industrial product development.
Further, this invention provides a new method for purification of rhMBL from the CHO cell culture media by using recombinant pre S protein as an affinity ligand. Further, this invention provides a convenient new method for determination of MBL activity by using recombinant pre S.
Further, this invention provides new formulations of MBL and methods of using them as triggers for complement activation. These formulations and methods provided are for treatment of acute patients with systemic infection by microorganisms.
Color was developed by using mouse monoclonal anti-human MBL (MBL8F6, 1:100 dilution, Dobeel) as first antibody and the second antibody was anti mouse IgG-HRP (1:1,500 dilution, KPL, USA). Color was developed with 150 μl/well of OPD (Sigma, USA), and color reaction was stopped with 50 μl/well of 3 M HCl. Color was measured by an automatic ELISA plate reader at 492 nm.
C4 (500 ng/well) cleavage was detected with anti-C4 antibody-HRP (1:1,500 dilution, Biogenesis, UK). Color was developed with 150 1 OPD as for the
This experiment was carried out by the same way as for
Using PLGA-pre S (amine group conjugated, N or carboxyl group conjugated, C), complement activation assay was carried out as for
2.5 μg rhMBL/mL culture media prevented SARS corona Virus infection to FRhK-4 cells.
General Description and Definitions
The practice of the present invention will employ, unless otherwise indicated, conventional techniques within the skill of the art in (1) culturing animal cells, microorganisms, viruses, and bacteriophage; (2) biochemistry; (4) molecular biology; (5) microbiology; (6) genetics; (7) chemistry. Such techniques are explained fully in the literature. See, e.g. Culture of Animal Cells: A Manual of Basic Technique, 4th edition, 2000, R. Ian Freshney, Wiley Liss Publishing; Animal Cell Culture, eds. J. W. Pollard and John M. Walker; Plant tissue Culture: Theory and Practice, 1983, Elsevier Press; Maniatis et al., Molecular Cloning: A Laboratory Manual; Molecular Biology of The Cell, Bruce Alberts, et.al., 4th edition, 2002, Garland Science: Microbial Biotechnology, Fundamentals of Applied Microbiology, Alexander N. Glazer and Hiroshi Nikaido 1995, W.H. Freeman Co.; Pharmaceutical Biotechnology, eds. Daan J. A. Crommelin and Robert D. Sindelar, 1997, Harwood Academic Publishers; �Manual of Clinical Laboratory Immunology, eds. Noel R. Rose et al, 4th Edition, 1997, American Society for Microbiology).
The present invention involves the discovery of a new oligomeric form of rhMBL, which, in a therapeutic context, is intended to trigger the complement system for the treatment of systemic infection with virus, bacteria, or fungus. For this purpose recombinant MBL has been produced from a uniquely constructed recombinant CHO cell line and formulated to fulfill the therapeutic purpose. General techniques for recombinant protein production can be found in Mammalian Cell Biotechnology in Protein Production, eds. Hansjorg Hauser et al, 1997, Walter de Gruyter, Inc.
As described below in detail, in this invention the MBL gene-transformed cell line was a host cell transformed with pMSG-MBL. pMSG-MBL was constructed from pMGS (Korean patent, KCCM 10202, hereby incorporated by reference), having a DNA sequence consisting of nuclear matrix attachment region element of beta-globin gene sequence, SV40 poly A, and transcription termination sequence of gastrin gene and MBL cDNA. The MBL cDNA was prepared from a human liver cDNA library by PCR method. The host cell line is an animal cell, preferably Chinese Hamster Ovary (CHO) cell, human hepatocytes, and/or human embryonic kidney (HEK) cell. Other host animal cell lines are familiar to those of skill in the art, and find use in this invention
In this invention as a non-limiting example, CHO cell was transformed with pMSG-MBL, and high expression clones were selected in the presence of increasing amount of methotrexate (MTX). The selected best clone was named CHO MBL/D1-3. The deposit of the MBL D1-3 (CHO cell line) was made on May 7, 2003, in the International Depositary Authority, Korean Collection for Type Cultures, located at Korea Research Institute of Bioscience and Biotechnology (KRIBB), #52, Oun-dong, Yusong-ku, Taejon 305-333, Republic of Korea. The deposited material was given accession number KCTC 10472BP. The deposit referred to herein will be maintained under the term of the Budapest Treaty on the International Recognition of the Deposit of Micro-organisms for purposes of Patent Procedure. This deposit is provided merely as convenience to those of skill in the art and are not an admission that a deposit is required under 35 U.S.C. � 112. A license may be required to make, use, or sell the deposited materials, and no such license is hereby granted.
MBL produced from the CHO cell line showed high molecular weight multimeric forms, resembling natural MBL purified from human serum. This invention presents ways to produce high molecular weight, multimeric form of MBL. Further the CHO cell line produced a large quantity of functional MBL, making it possible to use the clone for industrial quantity of MBL production.
This invention presents a convenient method for purification of MBL from CHO MBL/D1-3 culture media, using recombinant pre S as an affinity ligand. For example, first pre S was immobilized on an appropriate column matrix, next packed onto a column, after this the column was equilibrated with an appropriate buffer. Finally, the culture media with MBL was loaded on to the column in the presence of calcium ion, eluting out MBL with a buffer containing EDTA or EGTA.
The column matrix can be any such matrix as sepharose.
In other work, we constructed a recombinant yeast cell line that produced pre S portion of Hepatitis B virus envelope protein (International Publication No. WO 02/094866, herein incorporated by reference.)
Purified recombinant pre S from this cell is highly glycosylated, and bound with MBL, and activated MBL for complement activation in the presence of MBL free serum as a source of MASPs. Consequently pre S was a very useful tool for convenient assay of MBL for functional activity. Further, the pre S can be used as a mean for purification of MBL from serum as well as from other sources such as recombinant MBL production.
Recombinant pre S was also used in a specific formulation of MBL for a direct trigger for complement activation. We prepared a PLGA [Poly(D,L lactic-co-glycolic acid)]-particle coated with pre S, which bound with MBL and activated complement in the presence of MBL free serum. According to our finding pre S in a solution made neither stable complex with MBL, nor did it activate MBL for complement activation.
Equilibration of the column was accomplished by using a buffer that afforded best binding of MBL to pre S. This buffer contained calcium ion in the range of 2 mM to 20 mM. The source of MBL to be purified can be human serum or any other material containing MBL such as culture media of CHO MBL/D1-3.
Elution of MBL from the column can be done with any solution containing EDTA or EGTA in the concentration range of 5 mM to 10 mM. If necessary this affinity column step can be repeated.
This purified MBL finds use in formulation of therapeutic agents. Methods for formulating MBL in a pharmaceutical delivery vehicle for administration to subjects for prophylaxis and treatment of infection are known (NatImmune A/S, Copenhagen, Denmark). This can be a formulation for individuals with no or low level serum MBL to strengthen defensive measure against microbial infection. Further MBL can be used as a formulation of active therapeutic agent for patients with systemic infection, by administering a much higher dose of MBL than normal serum level of MBL. This formulation can be MBL so formulated as a direct trigger for complement activation to treat patients with systemic infection of microorganism.
Pharmaceutical delivery vehicle formulations can include water, buffer solution, and/or stabilizers. Stabilizers include glycerol, glucose, sucrose, sorbitol, trehalose, maltose, albumin, and amino acids such as lysine or glycine. The amount of one or combination of these can be 3% to 30%. (See Goodman & Gilman's The Pharmacological Basis of Therapeutics, eds. Joel G. Hardman, Lee E. Limbird, Tenth Edition, 2001, McGraw Hill; Basic & Clinical Pharmacology, Bernard G. Katzung, Eighth Edition, 2001, McGraw Hill; Pharmaceutical Dosage Forms and Drug Delivery Systems, Howard C. Ansel, Loyd V. Allen, Jr., Nicholas G. Popovich, Seventh Edition, 1999, Lippincott, William & Wilkins; Harrison's Principles of Internal Medicine by Eugene Braunwald M.D. (Editor), Anthony S. Fauci M.D. (Editor), Dennis L. Kasper M.D. (Editor), Stephen L. Hauser M.D. (Editor), Dan L. Longo M.D. (Editor), J. Larry Jameson M.D. (Editor).
Formulation of MBL can include MBL associated proteins such as MASP 1, MASP 2, MASP 3, and/or Map19. These MBL associated proteins can be from natural sources or recombinant proteins.
Formulation of an effective amount of MBL can be in the form of oil suspension, solution, or solid. In case of solid it can be dissolved in a solution mentioned above before administration.
The route of administration of MBL can be ip, sc, im or iv injection or a combination.
This invention provides method of making MBL for use as a composition which comprises a pharmaceutical delivery vehicle which is administered to a human subject in need of it as a trigger for complement activation. An embodiment of the invention is a preparation which uses pre S coated on the surface of liposome or PLGA nano-particle.
The term nanoparticles includes particles which are hollow as well as filled particles. Methods for making nanoparticles (5 to 500 mn), either uniform in size or having a predefined complex size distribution, from biocompatible, biodegradable polymers are well known. The sphere-shaped particles can be formed from a variety of materials, including FDA-approved, biodegradable polymers such as poly(lactic-co-glycolic acid) and polyanhydrides. (incorporated by reference are U.S. patent application 20020054912, U.S. Pat. Nos. 5,948,483, 6,060,128, 5,344,676, International Publication #WO 02/13786 A2; Precise control of PLG microsphere size provides enhanced control of drug release rate,� by C. Berkland, M. King, A. Cox, K. Kim, and D. W. Pack. Published in Journal of Controlled Release, 2002, 82(l):137�147; �Fabrication of PLG microspheres with precisely controlled and monodisperse size distributions,� by C. Berkland, K. Kim and D. W. Pack. Published in the Journal of Controlled Release, 2001, 73(1):59�74; �Visual evidence of acidic environment within degrading PLGA microspheres,� by K. Fu, D. W. Pack, A. M. Klibanov and R. Langer. Published in Pharmaceutical Research, 2000 17(1):100�106; �PLGA microspheres containing plasmid DNA: preservation of supercoiled DNA via cryopreparation and carbohydrate stabilization,� by S. Ando, D. Putnam, D. W. Pack and R. Langer. Published in Journal of Pharmaceutical Sciences, 1999, 88(1):126�130; Methods of preparing liposomes have been described (i.e., U.S. Pat. Nos. 5,653,996, 5,393,530 and 5,651,981, U.S. Pat. Nos. 5,595,756, 5,605,703, 5,627,159, 5,652,225, 5,567,433, 4,235,871, 5,227,170, all incoroporated by reference).
The size of nanoparticle ranges from 5 nm to 500 nm. The pre S can be replaced with any glycosylated protein or peptide or glycosyl moeity, e.g. mannose, that binds with MBL. Examples of glycosyl moieties include, but are not restricted to of mannose, N-acetyl glucosamine-rich oligosaccharides present on a wide range of bacteria, viruses, fungi, and parasites, glucans, and lipophosphoglycans and glycoinositol-phospholipids with mannose, glucose, fucose, or N-acetylglucosamine as N-terminal hexoses. Glycoproteins useful in the invention pre S glycosylated viral envelope protein, glycosylated bacterial protein, glycosylated fungal protein, i.e. and any artificially glycosylated protein which binds with MBL.
Free monomeric ligand such as pre S in solution does not activate MASPs. Whereas, pre S molecules immobilized onto solid surface make stable complex with MBL and activate MBL and complement system. Therefore our finding indicates that MBL complex activation is similar to C1 activation in that the activation of C1 complex requires engagement of multiple IgGs bound to cell surface antigens, but not with monomeric IgG molecule. Pre S conjugated onto the surface of PLGA nano-particle serves as an efficient activator of MBL complex.
This unique formulation of MBL as a trigger for complement activation can be broadly utilized for the treatment of patients with systemic infection by microorganism such as virus, bacteria, and/or fungus.
1.1 Construction of Expression Vector
MBL cDNA was prepared by PCR method using a human liver cDNA library, and it was cloned into pEZ vector (RNA, Inc. Suwon, Korea), pEZ-MBL2-5. The nucleotide sequence was verified from sequence stored in Gene Bank (Gene Bank NM�000242). Using this pEZ-MBL2-5 DNA as a template, 750 bp MBL cDNA for MBL expression was prepared by PCR method with primer 1 and primer 2 (see the sequence listing). These primers include Kozak Sequence and restriction endonuclease sites for cloning. This cDNA was cloned into pMSG vector (PanGen Biotech Inc., Suwon, Korea; Korean Patent, KCCM 10202) to make pMSG-MBL. The MBL sequence was verified again with Gene Bank sequence.
After transfecting pMSG-MBL DNA into an appropriate E. coli, the transformant was cultured in 100 mL LB media containing 100 ug/mL ampicilin. From this culture pMSG-MBL was prepared by using QUIAPREP Plasmide Midi Kit (Quiagen, USA). The pMSG-MBL linear DNA was prepared by digesting with Sca I.
1.2.2. Preparation of Host Cell
CHO DG44 (dhfr-/dhfr-) was cultured in -MEM media containing 10% cFBS and the cell number was determined with a hematocytometer. Then the cell number was adjusted to 2�105/mL cell in alpha-MEM containing 10% cFBS and cultured 24 hr in a CO2 incubator.
1.2.3 Transformation
A mixture containing 2 ug of pMSG-MBL DNA, 5.3 uL Dosper�, and 16 ng pDCH1P (Plasmid with DHFR gene, Venolia et al, 1987, Somat. Cell Mol. Genet. 13, 491�501) was incubated at room temperature for 45 min. Then the mixture was added to the host cell and incubated 6 hr. at 37 degree centigrade. After the incubation, media was removed and added 3 mL of fresh alpha-MEM containing cFBS. Then after 2�3 days when the transformed cells expanded enough, cells were harvested by trypsin treatment and 4�105 cell/well was grown in 2 mL of alpha-MEM without nucleosides containing 10% dialyzed FBS. Transformed cells were grown for about 10 days with media changes every 2�3 days.
1.3 Selection of MBL-producing Cell and Amplification of MBL Gene
Transduced MBL gene in the transformed cell was amplified by adding gradually increasing amount of MTX in the culture media. For this, cell number was adjusted to 4�105/well and cultured in the presence of 10 nM MTX until the cells reached confluent state. By a similar method, the concentration of MTX was increased to 1 uM. In each step the level of MBL expression was determined by Western blot analysis using anti-MBL. The level of MBL was increased as the concentration of MTX was increased, and a single clone was selected from the cell adapted to 1 uM MTX.
1.4 Selection of a Single Best Cell (Cloning)
For single cell cloning, 1 uM MTX adapted cells were distributed into a 96-well plate at 0.5 cell/well. This plate was cultured in alpha-MEM containing 10% dialyzed FBS and 1 uM MTX. About two weeks later when a single cell colony was formed, the cells were transferred to 24-well plate. When cells were expanded enough, cells were frozen after analyzing the expression level. One of the cell lines selected was CHO MBL/D1-3.
1.5 Estimation of MBL Expression Level
Using CHO MBL/D1-3 cell line, the level of MBL expression was estimated by comparing to a known amount of purified MBL from human serum. 5�105 cells were grown in a T25 flask with alpha-MEM without nucleosides containing 10% dialyzed FBS. When cell density reached 90% confluency, the media was replaced with 3 mL of the same media containing 5% dialyzed FBS. After 4 days the culture media was diluted 10 fold and the amount of MBL in the media was estimated to be 50 ug/106 cells/day.
2.1 Preparation of Pre S-Sepharose Column
One gram of CNBr activated Sepharose 4B was suspended in 1 mM HCl solution and washed several times with the same solution. To this washed Sepharose 6.4 mg pre S (recombinant pre S from Yeast, patent; PCT/KR02/00820, hereby incorporated by reference) was mixed in a coupling buffer pH 8.3 containing 0.2 M NaHCO3 and 0.5 M NaCl to make final concentration of pre S to be 0.5�10 mg/mL. After incubating 2 hr at room temperature, blocking buffer pH 8.0 containing 0.1 M tris was added and left in the room temperature for 2 hr. Then pre S-Sepharose was washed with the blocking buffer and the amount of immobilized pre S was estimated by Western blot analysis.
2.2 Purification of MBL by Using Pre S-Sepharose Column
We have established a purification method by exploiting the nature of pre S binding to MBL. Pre S can be produced in large quantity from our recombinant yeast, which produces a large quantity of pre S in culture media. Pre S-Sepharose was packed into a column and equilibrated with MBL binding buffer containing 20 mM Tris pH 7.6, 150 mM NaCl, 10 mM CaCl2, and 0.05% Tween 20. The column was loaded with MBL in the CHO cell culture media or in serum and washed extensively with the loading buffer. Then the MBL was eluted from the column with elution buffer containing 20 mM Tris pH 7.6, 150 mM NaCl, 5 mM EDTA, and 0.05% Tween 20. In this manner, just one step yielded 99.9% pure MBL. See
Biological activity of rhMBL was determined for two different functions; 1) specific binding of rhMBL to glycosylated protein in calcium dependent manner and 2) activation of complement (C4 cleavage) in the presence of glycosylated protein and MASPs.
3.1 Binding of rhMBL to Glycosylated Protein
3.1.1 Mannan Binding
So as to coat the plate with mannan, mannan solution in 50 mM carbonate-bicarbonate buffer was added to each well to make 1 ug/well on a Nunc Maxisorp Immunoplate and incubated overnight at 4 degrees centigrade. It was washed 4 times with washing buffer containing 20 mM Tris pH 7.6, 150 mM NaCl, 10 mM CaCl2, and 0.05% Tween-20 and treated with 0.2% BSA for 1 hr. After washing the plate 3 times with the washing buffer, 1 ug MBL/well was added in 100 uL of binding buffer containing 20 mM Tris pH 7.6, 1M NaCl, 10 mM CaCl2, 0.1% BSA, 0.05% Tween-20. After 2 hr incubation at room temperature, the plate was washed 6 times with the washing buffer and color was developed. First the plate was incubated with mouse monoclonal anti-human MBL (Dobeel, MBL8F6, 1:100 dilution) for 2 hr at room temperature. Next added was anti-mouse IgG-HRP (1:1500 dilution) and incubated 1 hr at room temperature. Finally color was developed by OPD (Sigma, USA). The color development was stopped by adding 3M HCl and the color was measured at 492 nm using an automatic ELISA plate reader. Mannan binding activity of rhMBL was compared with native MBL (
3.1.2 MBL Binding to Pre S
Employing the same method for MBL binding to mannan, MBL binding to pre S was carried out using 1 ug pre S (
3.2 Complement Activation by rhMBL
3.2.1 Complement Activation with Mannan Coated Plate
Nunc Maxisorp Immunoplate wells were coated with 1 ug/well mannan as described in 3.1.1, and this plate was incubated 2 hr at room temperature with rhMBL or serum MBL. When rhMBL was used the MBL free serum was added as a source of MASPs. After 2 hr incubation, the plate was washed 6 times with the wash buffer and incubated 2 hr at room temperature with 500 ng/well C4. Then it was incubated 1 hr at room temperature with anti C4 antibody-HRP and color was developed by adding 150 uL OPD. After 20 min. the color reaction was stopped by adding 50 uL/well 3M HCl and OD was determined at 492 nm. This experiment showed that rhMBL had comparable activity in terms of C4 cleavage to serum MBL (Data not presented).
3.2.2 Complement Activation with Pre S Coated Plate
Similarly to 3.2.1 with pre S coated (1 ug/well) plate, MBL activity for complement activation was determined. The result showed that pre S was better than mannan in the activation of MBL associated serine protease (
4.1 Preparation of Pre S Coated Nanoparticle
4.1.1 Preparation of PLGA Nanoparticle
Poly(D,L-lactic-co-glycolic acid) [PLGA], molecular weight ranging from 1,000 to 100,000, was dissolved in an organic solvent such as methanol or methylene chloride. It was then dispersed on a solution of SDS or Tween and kept overnight with violent stirring. Nanoparticles were recovered from this.
4.1.1 Conjugation of PEG to the Nanoparticle
Poly(ethylene glycol) [PEG] with heterofunctional reaction groups (X-PEG-Y) was used. In an organic solvent or in water solution, the reaction group X was activated and then conjugated to reaction groups (�OH, �COOH) on the nanoparticle. Linkers (X�) used in this conjugation can be as shown in the following:
Conjugation of pre S can be accomplished through -amine on lysine and arginine or N-terminal amine. Alternatively C-terminal or -carboxylic acid can be used to make conjugation of pre S to PEG conjugated to the nanoparticle. In these free Y reaction group on PEG can be conjugated to the carboxylic acid or amine on the pre S. In this the linker (Y�) can be as following:
Details of the procedures for protein conjugation to PLGA nano-particles are described in H. S. Yoo et al, J. Controlled Release, 82, 17�27, 2002 and S. H. Choi and T. G. Park, Intl. J. Pharmaceutics, 203, 193�202, 2000. The number of pre S conjugated to one PLGA particle is 100,000�300,000 pre S molecules/particle.
4.2 PLGA-pre S as a Functional Molecule for MBL Activation
Binding of rhMBL to PLGA-pre S and activation of complement by rhMBL-pre S-PLGA complex were carried out by the same methods employed in
Infection of SARS-CoV on fetal rhesus kidney cell (FRhk-4) was carried out in the presence of various amount of rhMBL in the culture media. As shown in the histogram of
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Senior Member
Better play HoN?
Ofc lol is more played.
But then again a lot of people listen to Justin Beaver.
Ssraeshza Tvyl
As far as I know, LoL has always had a larger playerbase than Dota.
Figures that I've seen recently, seem to indicate that the gap is increasing with time.
HOWEVER, do note that Dota 2 is currently in beta phase... so this may change drastically in the future.
Obviously LoL because Dota 2 is still in development.
I am nunu bot
I anticipate Dota2's population will skyrocket once it's in full release.
© 2014 Riot Games, Inc. All rights reserved. Riot Games, League of Legends and PvP.net are trademarks, services marks, or registered trademarks of Riot Games, Inc.


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1195cc, 150bhp, 140mph, Insurance group
The new 1190 is radically different from the 990 it replaces. The whole package of chassis, electronic suspension and rider aids makes this 150bhp, 230kg bike seriously capable with more rider-friendly characteristics than any previous KTM Adventure. It can also be ridden faster than I ever thought possible – in this respect it's more like a Ducati Multistrada, but with ...
1195cc, 175bhp, 186mph, Insurance group 17
KTM have listened to the criticism of poor fuelling and harsh throttle response and come up with a heavily revised package for 2011. More power, better fuelling with the same stunning looks and responsive handling. No electronic rider aids make it rewarding and engaging to ride either on or off the track.
999cc, 106bhp, 130mph, Insurance group 13
The KTM 990 Adventure is already a brilliant adventure trail bike, but the 2009 adds a little bit more refinement and power. A new crank has smoothed vibes, and touch extra power is not instantly noticeable but welcome all the same.
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Guyana Times article.
GEORGETOWN, GUYANA - Guyanese batsmen dominated with the bat in the opening round of the 2013 Toronto and District Cricket Association Elite 50-over League over the weekend at various venues across the city.
GEORGETOWN, GUYANA - Linden's Christianburg Wismar Secondary School cricket team has been selected as one of the teams from Guyana to represent the country at the 27th annual Sir Garfield Sobers Under-19 Cricket Tournament in Barbados from July 8-26.
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DERBYSHIRE, ENGLAND - Only nine overs were possible as persistent rain forced an early abandonment to Derbyshire's Yorkshire Bank 40 fixture with Surrey at the County Ground.
TORONTO, CANADA - "He was considered a great gentleman and his profound passion for cricket will always be remembered."
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DERBYSHIRE, ENGLAND - It was a case of Chanderpaul v Chanderpaul when Stainsby Hall CC took on a Derbyshire CCC XI last week.
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I want to buy some quince!
Anyone have a grocery store or ethnic food market near them that is still selling quince? My favorite grocery store, Dean's Produce in Millbrae, had them up until a month or so ago, at which point they told me that the season's over -- I'm no quince-grower, but I think the season goes a few months yet, and as I'm hoping to make some quince jam for stocking-stuffers, I'd love it if some other 'hounds could hook me up with a recent quince-sighting around the city.... (Ideally I'd like to find them at a small ethnic food store, and not some schnazzyfazzy market -- but given that I'm not finding them anywhere, I'll take any suggestions you have... thanks!)
Sorry. I'm looking out the window at my fruit-less almost-leafless tree. Quinces do store well, but even so I doubt you'll find any. Maybe use apples instead?
re: Dave MP
Dave, is the Alemany Farmer's Market different from Kuk Jea? I ask because I called Kuk Jea, and they said they didn't carry them (I even had a friend ask in Korean, because they didn't know what I was talking about when I asked about quince)... Still searching....
re: uberslop
Oops. Yes, totally different.
I had tagged Kuk Jea since I thought I had seen them there....but then I deleted that part of the post, but I guess I forgot to untag it. Sorry for the confusion.
Alemany Farmer's Market is the Saturday market in SF.
re: gordon wing
Oops, here's the link:
re: gordon wing
Monterey Foods
1550 Hopkins St, Berkeley, CA 94707, USA
Monterey Foods
1550 Hopkins St, Berkeley, CA 94707
Thank you all so much! I'll be calling around, driving around, and hopefully making some quince jam....
re: Emilie.
They still have 'em. I bought some today.
Well, I seem to be quite wrong! Do you all think these are local quinces that have been hanging around or are they from elsewhere?
I don't think you're wrong, exactly, Glencora -- but I think we're perched right on the cusp of the season. The main variety for quince in these parts is the Pineapple (most of the ones we see are grown south of Fresno), and supposedly the season runs from August into January or February -- but in those last two months I think they're only coming from South America, and so only available at specialty stores....
In case anyone else is looking, I just found some good ones for 2.99 a pound at Noriega Produce, out in the Outer Sunset.
Noriega Produce
3821 Noriega St, San Francisco, CA
And -- last update, I promise -- I found some even cheaper ones at El Chico # 4, also in the Sunset. 1.99 a pound.
El Chico Produce Market 4
2965 24th St, San Francisco, CA 94110
I'm a little confused by "Quince season"
A girlfriend of mine in Sausalito has a tree that bears a good amount of fruit but it seems to bear it at a different time each year. I've seen ripe fruit as early as mid-October and as late as early January and it never seems to be the same from year to year.
Nonetheless, poached in some white wine with ginger, turbinado sugar and
a little blood orange peel they are exceptional, and the syrup cooks down to a heavenly glaze.
re: jungleboy
that (yr quince-poaching) sounds awesome, jungleboy. and if she's got extra in mid-January this year, I'm buying....
I noticed some okay-looking quinces today at Casa Lucas on 24th St. 79 cents each, grown in California.


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one (male or female) whom enjoys the act of puffing or lightly blowing into a twat.
Yo mayne, you see that girl lightly blowin twat last night?!" "Shit son i knew i saw a twat puffer i think it was EMILY NEWMAN
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The Office of Community and Economic Development at Fresno State is proud to announce its new hires for fall 2014: Marianne Collins King as associate director, operations; Emily Pasquarelli as administrative assistant; Jenna Chilingerian as program assistant; Karmjot Grewal as program manager; and Jena Rodriguez as communications assistant.
Marianne Collins King hails from the Silicon Valley in Northern California. She was the principal of the consulting firm Collins King Communications and has 15 years' experience working with a multibillion dollar insurance company where she developed a succinct background in the realms of alternate dispute resolution, mediation, litigation, conflict resolution, and case management. Currently Marianne serves as board member for the following nonprofits: Face Forward, Inc., MadeForThem, and the Fresno Art Museum. She is a member of the Fresno Downtown Partnership Association, Fresno Historical Society, Association of Junior Leagues International, Society for Human Resource Management, and Central California Society of Human Resource Management. Marianne earned her Bachelor of Arts in business management with additional concentrations in human resources, employment law, organizational development, and psychology from Saint Mary's College of Moraga, California. She is currently pursuing a doctorate degree in organizational behavior and a dual degree in international business.
Emily Pasquarelli is a native of Fresno who returned to the area after spending 20 years in the film production industry in Los Angeles. She has extensive expertise managing offices and creating more efficient and functional work environments. Prior to joining OCED, Emily was administrative assistant at Fresno's Central Valley Business Incubator. She earned a Bachelor of Arts in mass communication and journalism, with an emphasis in radio, television and film from Fresno State.
Jenna Chilingerian, a native to Fresno, received her Bachelor of Arts in political science from University of California, Los Angeles in 2013, with a minor in civic engagement. She has held several internships in law firms, local governments, and nonprofits. As a program assistant for the Center for Community Progress in Washington, D.C., she worked closely with the vice president and director of National Leadership and Education to create a community of practice around solving vacant and abandoned property problems. Most recently, Jenna interned with the U.S. Conference of Mayors in Washington, D.C., and with the City of Fresno in the offices of Mayor and City Manager.
Karmjot Grewal is a native of the Central Valley where she earned both her Bachelor of Arts in psychology and Masters of Arts in research psychology from Fresno State. In addition, she earned an MBA with an emphasis in organizational development from Johns Hopkins University in 2014. Prior to joining OCED, Karmjot was a program analyst at the U.S. Department of Education headquarters in Washington, D.C., where she worked closely with the Small Business Innovation Research (SBIR) program.
Jena Rodriguez, a native of the Central Valley, was raised in the small farming community of Corcoran. Prior to joining the OCED team, Jena was an intern and volunteer for various nonprofit organizations in the Valley. Her experiences include social media, public relations, events, and communications. She earned her Bachelor of Arts degree in mass communication and journalism with an emphasis in public relations from Fresno State in 2014.
For more information, contact Mike Dozier at 559-294-6021 or firstname.lastname@example.com.


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Originally written on Wrestlechat.net | Last updated 10/21/14
- WWE officials are going back and forth on whether or not the Ryback vs. CM Punk TLC match will actually take place at the January 7th RAW. With The Rock in the house that night, should they do the match, the plan would be to have The Rock at ringside. The idea is that they could hype the match up all night with the idea that it could be Ryback going on to the Royal Rumble to defend against The Rock. A source says to expect at least one segment with The Rock and Ryback that night. - On a related note, it's said that The Rock is perfectly fine with dropping the WWE Title to John Cena at WrestleMania 29. It's been reported Rock has been under the impression that he will be the one to end CM Punk's title reign at the Royal Rumble.
Original Story: http://wrestlechat.net/latest-on-ryba...
PLAYERS: John Cena
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Yardbarker is the largest network of sports blogs and pro athlete blogs on the web. This site is the hub of the Yardbarker Network, where our editors and algorithms curate the best sports content from our network and beyond.


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