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Italian. 90 members
English. 222 members
First: How are you?
Second: How disgusting!
Third: Happy anniversary! Cin cin! Are you happy? That's fab!
I rarely go to a bar, because I usualy am very busy on my family and on my job, but when it is holidays I celebrat
Tomorrow is our father's day. We are buying him a present. I think a watch is a perfect present for him, but my b
We don't go out on Halloween. Only the boys and girls go out for Celtic party. We go to the church and to the ceme
My son plays with her playstation in her free time. I talk him: Please, can you study? You have to do an exam tomo


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In yesterday's Wall Street Journal, Geeta Anand and Betsy Mckay explained how the health authorities in the rapidly developing BRICS nations have agreed to work together to fight tuberculosis.
The BRICS–Brazil, Russia, India, China, and South Africa–account for about 60% of the world's multi-drug resistant TB cases, so it is good news that they see combating MDR-TB as a policy priority.
In a journal article my team published today, we find that about one in ten TB medicines is substandard, which will significantly contribute toward drug resistant strains of TB. And we find that 63% of those substandard medicines probably come from BRICS countries. If the BRICS really want to combat MDR-TB, they should ensure that fewer fake and substandard medicines are to be found in their nations.


text: PCToday New Experiences, Old Friends Homecoming 2014, a spectacular continuation of Penn College's Centennial celebration, made institutional history while honoring it.Read more One College Avenue 'Winning Their Share'To help train men and women for war-related production, the institution overhauled its curriculum from 1940-45, reinforcing a growing national reputation.Read more Athletics Go Wildcats!Visit the Athletics website for highlights, scores, schedules, and features. Read more Video Wildcat Athletics: Tennis The tennis program is highlighted with the help of team member and building construction technology major Nathan Scott. Watch video RECOGNITION Veterans welcome Penn College is recognized as a Military Friendly School by G.I. Jobs. Read more CELEBRATION Centennial Nearly 100 years of history, from Williamsport Technical Institute (WTI) to Williamsport Area Community College (WACC) to Pennsylvania College of Technology. Learn more Video Associate Degrees in Demand A wide variety of employers seek two-year students for rewarding career opportunities. Watch Video RECOGNITION Among the "top 10"... … best public, four-year colleges in the north region of the United States, according to America's Best Colleges by the U.S. News Media Group. Learn more


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Thursday, July 10, 2014
Community | Obits | Editorial & Columnists | Society | Sports | Education | Classified Ads | Calendar of Events | Features | Newsbriefs | Legals | Archives | Subscriptions | New Photo Gallery
Click Here for the E-Edition of
The South Reporter
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Calendar of Events
July 9
• Revival, Hopewell No. 1 MB Church, 365 Bonner St., Holly Springs, July 8-10, 7 p.m. nightly. Evangelist Rev. Dr. C. Shelton Greer Sr., pastor of Hopewll MB Church, Independence. Rev. Leroy James, host pastor.
July 10
• Relay for Life of Marshall County will celebrate our Wrap-Up at 5:15 p.m. at the Marshall County Fairgrounds. Come with celebration on your mind. Invite survivors to come too. We will have food and prizes.
• The Lessye Lee Davis 4-H Club will meet at the Marshall County Library on July 10 at 6 p.m.
• The Benton County Commission on Aging and Independent Living, LLC. will offer adult day program services starting in 2014; and assisted living facility services in 2015. The next Community Awareness Day is July 10, 11 a.m.-2 p.m. at the old Ashland Library. For more information, visit us at www.bentoncca.org or 662-224-6319.
July 11
• Ida B. Wells-Barnett Museum will host its annual Festival and Celebration, July 11-13. For more information and a schedule of events, call the museum at 662-252-3232 or Leona Harris, museum director at 662-551-0296.
• The Victoria United Methodist Church, Victoria, will hold a yard sale, July 11 and 12 from 8 a.m until 4 p.m. at the church parking lot.
•Watson Fire Dept., Rummage Sale, Fri. and Sat., 8 a.m.-2 p.m. Rain or shine! The proceeds go to the Watson Fire Dept Building Fund. We are still in the same place, 121 Watson Road, next door to the Watson Fire Dept, at the old Watson Saddle Club.
July 12
• Second annual "Day of Jubilee Gospel Explosion," Pleasant Hill CME Church. A community choir event, and all young adults in the area are invited to come to rehearsal.
• Pleasant Hill CME Church choir along with others forming the Unified Mighty Voices of N.E. Mississippi, will host a community choir concert at 5:30 p.m. Rev. Willie Hoey, pastor, invites everyone.
• New Hope Baptist Church, 137 Valentine Rd., Holly Springs, Bro. Tony Watkins and congregation invite you to their monthly singing, featuring Beyond Measure. Fellowship meal will be at 6 p.m. and singing will start at 7 p.m.
• Mustard Seed Ministries, 3213 Hwy. 309 S. Byhalia, will have a Rummage Sale/Bake Sale, 7 a.m. to 2 p.m.
• Registration for Victoria United Methodist Church in Victoria, Vacation Bible School will be held on Saturday, July 12 from 2 until 4 p.m.
July 13
• Church Anniversary will be held at Pleasant Hill CME Church, 2:30 p.m. Rev. Eldrick Garrison of Jones Chapel MB Church will be the guest. Rev. Willie Hoey, pastor, invites everyone.
• St. Paul C.M.E. Church, Holly Springs, will have their Pastor's Love Day at 2:30 p.m. Rev. Robert Odom and Wilkins Chapel CME Church will be the guests. Rev. Charles Dooley is the pastor. Sis. Kathy Pegues is the program chairperson. The public is invited.
• Emory Independent Methodist Church will be holding their revival July 13 through July 16. There will be a dinner on July 13 following the 11 a.m. service. Sunday evening services will be held at 6:30 p.m., Monday through Wednesday services will be held at 7 p.m. The MYF will hold an ice cream social after the services on Wednesday evening.
• Rice Chapel Methodist Church will have revival, beginning 11 a.m. Brother Walter Myers will be the guest speaker. The Almost Famous Craines will perform after lunch.
July 14
• Rice Chapel Methodist Church revival begins at 7 p.m. Anita Lawson will be the guest speaker. Tuesday and Wednesday night, Tony Watkins will be the guest speaker. Rev. Wayne Sheffield invites everyone.
• Chewalla Assembly of God hosts VBS "Wilderness Adventure", July 14-18, 6:30-8 p.m. There will be snacks, games, arts and crafts for ages K5-5th grade. The church is located at 1511 Higdon Road, Holly Springs.
July 15
• Vacation Bible School will be held July 15-17 from 5:30 until 8 p.m at the Victoria United Methodist Church, Victoria, (behind the post office). We invite all the children in the community to come and take part in this time of singing, worship, crafts and lots of fun.
July 17
• The Marshall County Humane Society meeting will be Thurs., July 17, 6 p.m. at the Adoption Center on Eddie Smith Dr. Bring your animal-loving friends and come on down.
July 18
• Basketball tournament, Holly Springs Parks and Recreation. Fee will be $80, three-on-three basketball, up to four players per team. Contact info. Sanchez Blake, 662-544-6163 or 662-252-4807, ext. 1601.
July 19
• Pastor and Wife's Love Day Celebration for Rev. Willie Hoey and Sis. Cathlean Hoey will be held at Pleasant Hill CME Church at 6 p.m. Elder Pass and Christ Temple will be the guest. Rev. Willie Hoey, pastor, invites everyone to attend.
July 20
• Hebron Chapel CME Church will have a Missionary Program hosted by Linda Mathis, 2:30 p.m. The guest speaker will be Velicia Howard. Pastor, Rev. Dr. Edwards, invites everyone. The church is located at 2827 Hernando Road. The theme is "Bringing Good Fruit. The Fruit of the Spirit."
• St. Mark MBC Usher Day will be held at 2:30 p.m. Guests will be Rising Star, and the speaker will be Pastor Robert McClaire. Everyone is welcome.
• Providence M.B. Church will celebrate their annual Musicians Appreciation at 2 p.m. We extend an invitation to choirs, groups and soloists to take a part. Alonzo Johnson is pastor. The church is located at 295 Withers Ave., Holly Springs. Please contact choir secretary Lillian Johnson at 551-8389 for additional info.
• Greenfield Presbyterian Church, Waterford, will have a mortgage burning ceremony, 3 p.m. Everyone is invited. Pastor is W. James Lyons.
• New Hope M.B. Church, 3120 Hwy. 310, Laws Hill, will have their Men's Day Program, 11:45 a.m. Guest speaker will be Pastor Devin Westbrook and Redemption Life Church. Pastor Dr. Bessie Tables invites everyone.
• Hopewell Baptist Youth Choir will be in concert at Carey Chapel Baptist, 6 p.m. All are invited to come enjoy this group.
July 21
• Emory Independent Methodist Church will be having Vacation Bible School will be July 21-July 25, 5:30 p.m.-8 p.m. Classes will be for nursery through sixth grade. A light meal will be served for the children attending the Bible school.
July 24
• The Lessye Lee Davis 4-H Club will meet at the Marshall County Library on July 24 at 6 p.m.
July 25
• We are asking all classmates of W.T. Sims Class of 1969 to phone in your current mailing address, telephone number and e-mail address to Mable Malone Lester at 662-287-8437. We will be notifying everyone about the upcoming dates for our 45th Class Reunion. Please reply by July 25, 2014.
July 30
• New Hope M.B. Church will host an old-fashioned revival, July 30, 31 and August 1, 7 p.m. nightly. The church is located at 3120 Hwy. 310, Waterford. Wed.: Ladies' Night-Minister Milony Jenkins and Mt. Moriah Women's Choir; Thurs.: Youth Night-Minister Jeremie Bowen and Mt. Moriah Youth Ensemble; Fri.: Men's Night-Pastor R.J. Wilson and Mt. Moriah Mass Choir.
August 5
• Mt. Moriah Missionary Baptist Church, 3193 Marianna Road, will have a Revival Crusade, Aug. 5-7, 7 p.m. nightly. Guest evangelist Pastor Evelyn Hubbard. Rev. R.J. Wilson, pastor.
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text:
JCECE Result 2012 - Jharkhand Combined Entrance Competitive Examination Results - Jharkhand JCECE Medical Result 2012 - JCECE Engineering Result 2012 - JCECEBOARD Result 2012
Jharkhand Combined Entrance Competitive Examination Board (JCECEB) Will Be Announced Soon
Result of JCECE 2012 on JCECEBOARD Official website .
JCECE Test 2012 Was Held For Take Admission in Various Degree Courses Like as Engineering , Medical And Agriculture Subject Under Jharkhand State .
All Those Candidates Who has Appeared in JCECE Examination 2012 . Which Examination Was Held
in may month 2012 Can Be Check Their JCECE Result 2012 From Following Given below When Official
website Will be Announced .
Allgovtjobs.in Teams Wishes All The Best To All Students Who Has Appeared in JCECE 2012 Examination


text: Historically, most collarbone fractures have been treated without surgery and have simply been allowed to heal. New studies, however, have shown that in many instances patients do better with surgical treatment. Specially-designed plates and pins are now available to fix collar bone fractures properly so that the bone heals in its original position. In such instances, surgery will provide a more predictable outcome in terms of shoulder motion and strength.


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You can hear some birds singing ans some people talking. In the background you can hear the typical sound of ...
Just a recording of me screaming. Recorded on a AT3525
Another scary scream.
A scary scream I recorded when looking at a horrorfilm in school. This one is clipping but it can work, ...
A scary scream I recorded when looking at a horrorfilm in school.
Another scary scream.
My friend's screaming.
Individual children screaming
girl scream
This is me screaming into the microphone.
Scream recorded on a sm58.
The result of tinkering with -zin-'s "piano_2_140bpm.wav". Created using a lot of reverb, tremolo, and echo effects.
a dark and distorted riser pad sample
a dark, distorted and echoed percussion sound
A recording made at dawn in Canggu, south Bali.


text:
The original mantras on which the YACHT album See Mystery Lights is based have never before been released online. They were performed with audiences several times at special events in 2009 and 2010, and included with the eBook version of The Secret Teachings of the Mystery Lights. We are very lucky to announce that we've procured audio recordings of the mantras and, with YACHT's approval, are releasing them here online. This is the first:
Where Does The Spirit Dwell/
Not In Heaven/
Not In Hell
It forms the ideological basis of the song "Ring the Bell."


text:
Miracle City
Luckiest Productions in association with Hayes Theatre Co will present a brand new production of Nick Enright and Max Lambert's musical Miracle City at Hayes Theatre Co in Sydney from October 17 - November 16 2014.
Set in real time during a live-to-air American evangelical television show, Miracle City tells the story of the Truswells, a family with a dream to build a religious theme park, and the extreme lengths the patriarch Ricky Truswell will go to in order to make their dream a reality. Miracle City had its world premiere at the Sydney Theatre Company in 1996 to huge acclaim.
Miracle City will be directed by Darren Yap - The Serpent's Table, A Murder is Announced - with musical direction by Max Lambert - Hairspray, Strictly Ballroom - and choreography by Kelley Abbey - Fame The Musical, Xanadu: The Musical.


text:
- freely available
- re-usable
Int. J. Mol. Sci. 2014, 15(4), 6046-6061; doi:10.3390/ijms15046046
Published: 10 April 2014
Abstract: In this retrospective pilot study, the expression of the prostate-specific membrane antigen (PSMA), the epithelial cell adhesion molecule (EpCAM), the vascular endothelial growth factor (VEGF) and the gastrin-releasing peptide receptor (GRPR) in locally recurrent prostate cancer after brachytherapy or external beam radiotherapy (EBRT) was investigated, and their adequacy for targeted imaging was analyzed. Prostate cancer specimens were collected of 17 patients who underwent salvage prostatectomy because of locally recurrent prostate cancer after brachytherapy or EBRT. Immunohistochemistry was performed. A pathologist scored the immunoreactivity in prostate cancer and stroma. Staining for PSMA was seen in 100% (17/17), EpCAM in 82.3% (14/17), VEGF in 82.3% (14/17) and GRPR in 100% (17/17) of prostate cancer specimens. Staining for PSMA, EpCAM and VEGF was seen in 0% (0/17) and for GRPR in 100% (17/17) of the specimens' stromal compartments. In 11.8% (2/17) of cases, the GRPR staining intensity of prostate cancer was higher than stroma, while in 88.2% (15/17), the staining was equal. Based on the absence of stromal staining, PSMA, EpCAM and VEGF show high tumor distinctiveness. Therefore, PSMA, EpCAM and VEGF can be used as targets for the bioimaging of recurrent prostate cancer after EBRT to exclude metastatic disease and/or to plan local salvage therapy.
1. Introduction
Prostate cancer is the most commonly diagnosed cancer among men, and its incidence rates remain as the highest in many regions of the world [1]. About 18% of patients with localized disease develop a prostate-specific antigen (PSA) recurrence within five years after brachytherapy and external beam radiotherapy (EBRT) with doses higher than 72 Gy. This is in contrast to 49% of patients who were treated with doses less than 72 Gy [2]. At present, there is no imaging modality that can accurately discriminate between locally and distant recurrent prostate cancer after treatment with curative intent. Selecting patients for salvage cryotherapy of the prostate or salvage prostatectomy by excluding distant metastases proves a diagnostic challenge.
New diagnostic techniques are needed to improve the imaging of recurrent prostate cancer. Furthermore, although early-stage and locally recurrent prostate cancer can be cured, treatment of metastasized disease is currently only palliative, making it important for new therapeutic applications to be devised [3].
Significant expression of certain antigens in prostate cancer as compared to normal tissue could be used for antigen-targeted imaging or therapy. Numerous pre-clinical and clinical trials focused on this topic have already shown promising results [4–8].
Among the antigens used for diagnostic applications, the prostate-specific membrane antigen (PSMA) was demonstrated to be a useful target [9–13]. PSMA is a Type II integral membrane glycoprotein, which is overexpressed in prostate cancer in comparison to benign prostate tissue [11,14,15]. Next to expression in prostate, PSMA was found to be expressed in the neovasculature of solid tumors in comparison to normal vasculature [16,17]. The biological role of PSMA is not completely understood [10,18,19].
Ross et al. demonstrated a significant correlation between PSMA expression in prostate cancer and the Gleason score, pathological stage and biochemical recurrence [11]. Indium-111 capromab pendetide (ProstaScint®) is a radiolabelled antibody directed against PSMA. Correlation of scan results with pathological specimens suggests that ProstaScint is able to detect soft tissue metastases [20–23]. However, for routine use in clinical practice, the sensitivity of ProstaScint is not high enough, because the antibody targets the intracellular epitope of PSMA, thereby probably targeting only damaged or necrotic/apoptotic cells. Furthermore, the role of ProstaScint in the diagnosis of recurrent disease has to be elucidated [24].
Another antigen that can be used as an imaging target is the epithelial cell adhesion molecule (EpCAM). EpCAM is a transmembrane glycoprotein, which is highly expressed in rapidly proliferating tumors of epithelial origin [25–27]. This protein is found to be strongly expressed in several carcinomas [28–31]. In normal epithelium, there is a lower expression of EpCAM [32]. EpCAM mediates epithelial-specific intercellular cell-adhesion. Next, it is suggested that EpCAM is involved in cell migration, signaling, proliferation and differentiation [33]. The expression of EpCAM is inversely related to prognosis in several carcinomas [33]. For prostate cancer, this relation is controversial [32,34].
Signal protein vascular endothelial growth factor (VEGF) and its receptors are involved in (tumor-related) angiogenesis [35,36]. VEGF is overexpressed in a variety of tumors, including gliomas, breast, renal cell and hepatocellular cancer [37]. VEGF is a potential target, as its expression has also been demonstrated in prostate cancer [38,39]. The expression of VEGF in normal prostate, benign prostate hyperplasia and prostate cancer in relation to tumor grade is inconsistent in the current literature [7,40–49]. As for EpCAM, the prognostic value of VEGF expression is controversial [50–53].
The gastrin-releasing peptide receptor (GRPR) can be a promising imaging target. GRPR is a glycosylated seven-transmembrane G-protein coupled receptor, which is expressed in numerous cancers, such as those of the lung, colon and prostate [54–59]. GRPR seems to be overexpressed in prostate cancer in comparison to sparse expression in normal prostate tissue [60–62]. Binding of GRPR stimulates the growth of prostate cancer cells in vitro and in vivo [63,64]. A significant inverse correlation was found between GRPR expression and an increasing Gleason score [60].
Currently, there is no knowledge about the expression of PSMA, EpCAM, VEGF and GRPR in locally recurrent prostate cancer after brachytherapy or external beam radiotherapy. Therefore, the aim of this pilot study was to investigate the expression of these antigens using immunohistochemistry and to analyze their potency for new diagnostic applications in locally recurrent prostate cancer.
2. Results and Discussion
2.1. Results
In Table 1, the results of the immunohistochemical staining of different antibodies in prostate cancer specimens are presented.
Overall, staining for PSMA was seen in 100% (17/17), EpCAM in 82.3% (14/17), VEGF in 82.3% (14/17) and GRPR in 100% (17/17) of prostate cancer specimens. Staining for PSMA, EpCAM and VEGF was seen in 0% (0/17) and for GRPR in 100% (17/17) of the specimens' stromal compartments. Immunohistochemical staining intensity frequency, number and percent are shown in Table 2.
In 11.8% (2/17) of cases, the GRPR staining intensity of prostate cancer was higher than that of stroma. In 88.2% (15/17) of cases, the GRPR staining intensity of prostate cancer was equal to the staining intensity of stroma. Tumor distinctiveness is shown in Table 3.
2.2. Staining Pattern
PSMA staining in prostate cancer specimens was membranous and cytoplasmic (Figure 1). Six cases with focal uptake amidst negative cancer tissue were observed (Patient No. 2–4, 6, 8, 13) (Figure 1). For PSMA, there was minimal staining of prostatic intraepithelial neoplasia (PIN) and normal prostate epithelium. EpCAM stained cytoplasm and at the basal membrane (Figure 2). VEGF and GRPR staining in prostate cancer tissue was cytoplasmic and diffuse (Figures 3 and 4). Staining of striated muscle was observed for GRPR.
2.3. Discussion
This study has shown that PSMA, EpCAM, VEGF and GRPR are expressed in locally recurrent prostate cancer after brachytherapy and external beam radiotherapy. Staining for PSMA and GRPR was observed in all prostate cancer specimens (17/17), while EpCAM and VEGF staining was observed in 82.3% (14/17) of cases (Tables 1 and 2). Staining for PSMA, EpCAM and VEGF was absent (0/17) in specimens' stromal compartments, while GRPR staining in stroma was observed in 100% (17/17) of cases. In 88.2% (15/17) of cases, the GRPR staining intensity of prostate cancer was equal to the staining intensity of stroma. Staining of striated muscle was observed for GRPR, which is in agreement with the findings of other groups [60–62].
The high expression of PSMA, EpCAM, VEGF and GRPR in locally recurrent prostate cancer after ionizing therapy could have several reasons, which will be postulated next. First, high expression of either antigen is correlated with a tendency for recurrence. This holds true for PSMA, as its expression in primary prostate cancer is positively correlated with factors, like a high Gleason grade, a higher tumor stage and, most important in this case, biochemical recurrence [11,18]. The same goes for EpCAM, as a recent study by Benko et al. showed that higher EpCAM expression correlated with a higher Gleason score and a shorter disease-free survival [34]. However, other research groups found conflicting results [26,30,65]. Expression of VEGF in prostate cancer positively correlated with high a Gleason score, lymph node metastases and the progression of disease [66]. Although there is no information about GRPR expression and recurrence, the process might be comparable.
Second is the upregulation of antigens due to radiotherapy. It has been demonstrated that VEGF is upregulated after radiotherapy for rectal cancer [67]. For EpCAM, PSMA and GRPR, this is currently unknown. Third, recurrent cancer could have the tendency for upregulation of certain antigens. In ovarian cancer, EpCAM upregulation was seen in recurrent cancer when compared to primary cancer in matched samples [68], while VEGF levels were significantly higher in recurrent acute lymphoblastic leukemia compared with newly diagnosed cases [69]. For all the postulations, we have no direct evidence, as we did not have prostate cancer biopsy samples before and after radiation therapy that could have been compared to the salvage prostatectomy samples.
The absence of non-specific background staining of normal tissue or prostate stroma for the anti-PSMA, EpCAM and VEGF antibodies proves the specificity for cancer tissue. Background staining for GRPR was evident. Although different protocols were used and different blocking agents were tested, staining of non-cancer tissue remained. Staining of normal prostate and muscle with the anti-GRPR antibody has been described in the literature by other authors [60–62], and at first glance, this might pose a problem for the future use of GRPR as a target for new therapeutic or diagnostic modalities. However, pre-clinical and clinical studies have shown very low uptake of GRPR-targeted bombesin-like radiopharmaceuticals in muscle with high tumor-to-muscle ratios [70].
The current study has several limitations. First, we did not have matched samples of prostate cancer tissue. In an ideal situation, we would have compared prostate biopsy samples at initial diagnosis before radiation therapy, the biopsy samples in which local recurrence was confirmed after radiation therapy and the salvage prostatectomy samples; Second, staining intensity was assessed qualitatively on a scale from zero to three. Although strong staining suggests a higher antigen density than weak staining does, it is impossible to make a comparison between the different antibodies used in this study, as different protocols were used and as there is most likely different antigen sensitivity for each antibody. A quantification of antigen-densities has to be performed to be able to make a comparison between antigens; Third, due to the low number of patients (n = 17), the power of the statistics would be too weak, and therefore, we were unable to correlate staining intensity with clinicopathological parameters.
Finally, we did not have the follow-up data to compare antigen expression with outcome.
There is not much knowledge about the expression of antigens in recurrent prostate cancer. Locally recurrent prostate cancer is, to our knowledge, an unexplored field. Therefore, this is the first study to show the data about the expression of PSMA, VEGF, EpCAM and GRPR in locally recurrent prostate cancer after ionizing therapy.
Antigen-based targeted imaging of the prostate could be useful in several scenarios: if salvage local therapy was planned without a biopsy or with repeated negative biopsies, but with a strong suspicion of local recurrence. Other applications for these imaging strategies would be to rule out micrometastatic disease prior to local salvage therapy or the application of salvage focal therapy only to areas of uptake on imaging in order to minimize side-effects in irradiated tissue. The unique overexpression on prostate cancer cells makes PSMA the most attractive target for the delivery of imaging agents [10]. Several clinical studies with PSMA as the target have been reported with encouraging results [71,72]. Bombesin-like radiopharmaceuticals, which are natural ligands of GRPR, can be relatively easy synthesized in large quantities and have shown promising results in several clinical studies [70]. However, due to its background staining, more careful selection of the protocol may be required for optimal targeting. Only a few studies consider VEGF and EpCAM-based diagnostics for targeted cancer imaging [73–75], but based on the results reported in this study, both antigens can be used for the detection of locally recurrent prostate cancer.
3. Experimental Section
3.1. Materials
Patients in our retrospective study were diagnosed with locally recurrent prostate cancer based on PSA relapse and transrectal ultrasound-guided prostate biopsies. Prostate cancer specimens were collected of 17 patients who underwent salvage prostatectomy (The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital Amsterdam (NKI/AVL)), because of locally recurrent prostate cancer after brachytherapy (4 patients) or external beam radiotherapy (13 patients). All tissue specimens were anonymous and encoded with a unique code. According to Dutch law, no further Institutional Review Board approval was required (http://www.federa.org). Pretreatment biopsies were not available.
3.2. Immunohistochemistry
Formalin-fixed, paraffin-embedded blocks of prostate tissue were cut into 4-mm-thick sections and mounted on Starfrost microscope slides. Hematoxylin and eosin stained sections were graded by an experienced pathologist (Stefano Rosati), based on the criteria of the Gleason grading system. Remaining sections were processed for immunohistochemistry.
After deparaffinization, antigen retrieval was performed by heating microwave (700 W) for 20 min in a 10 mM citrate buffer at pH 6.0, with a cool down period of 20 min afterwards. Endogenous peroxidase was blocked with 0.3% hydrogen peroxide in phosphate-buffered saline (PBS) for 20 min. Slides were than incubated with the primary anti-human-PSMA mouse monoclonal antibody, YPSMA-1 (Abcam, Cambridge, UK), diluted at 1:400 in 1% bovine serum albumin/phosphate-buffered saline (1% BSA/PBS) for 1 h at room temperature. The secondary step consisted of incubation with rabbit anti-mouse antibody conjugated to polymer-horseradish peroxidase (DAKO, Glostrup, Denmark), diluted at 1:100 in 1% BSA/PBS with 1% AB serum. For the tertiary step, goat anti-rabbit antibody conjugated to polymer-horseradish peroxidase (DAKO, Glostrup, Denmark) was used, diluted at 1:100 in 1% BSA/PBS with 1% AB serum. Both the secondary and tertiary step required incubation for 30 min at room temperature. Next, the slides were immersed for 10 min in a solution of 0.05% 3,3′-diaminobenzidine (Sigma-Aldrich, St. Louis, MO, USA) and 0.03% hydrogen peroxide in PBS for the visualization of the signal as brown staining. After washing with demineralized water, the slides were slightly counterstained with hematoxylin, dehydrated and mounted with Eukitt mounting medium (Sigma-Aldrich, Steinheim, Germany).
After deparaffinization, antigen retrieval was performed by heating microwave (700 W) for 20 min in 0.1 M Tris/HCl buffer at pH 9.0, with a cool down period of 20 min afterwards. Endogenous peroxidase was blocked with 0.3% hydrogen peroxide in Tris-buffered saline (TBS) for 20 min. Slides were then incubated with a normal goat serum diluted at 1:10 in TBS for 30 min at room temperature. Afterwards, the slides were incubated with the primary anti-human-GRPR rabbit polyclonal antibody, ab39963 (Abcam, Cambridge, UK), diluted at 1:250 in 1% BSA/TBS overnight at 4 °C. Only a secondary step with goat anti-rabbit antibody conjugated to polymer-horseradish peroxidase (DAKO, Glostrup, Denmark) was applied, diluted at 1:100 in 1% BSA/TBS with 1% AB serum for 60 min at room temperature. Next, the slides were immersed for 10 min in a solution of 0.05% 3,3′-diaminobenzidine (Sigma-Aldrich, Steinheim, Germany) and 0.03% hydrogen peroxide in PBS for the visualization of the signal as brown staining. After washing with demineralized water, the slides were slightly counterstained with hematoxylin, dehydrated and mounted with Eukitt mounting medium (Sigma-Aldrich, Steinheim, Germany).
3.2.3. EpCAM
After deparaffinization, antigen retrieval was performed by incubation with 0.1% protease for 30 min at room temperature. Endogenous peroxidase was blocked with 0.3% hydrogen peroxide in PBS for 20 min. Slides were than incubated with the primary mouse monoclonal anti-EpCAM antibody (Clone VU-1D9, Leica Biosystems, Newcastle, UK) diluted at 1:100 in 1% BSA/PBS for 1 h at room temperature. The secondary step consisted of incubation with rabbit anti-mouse antibody conjugated to polymer-horseradish peroxidase (DAKO, Glostrup, Denmark), diluted at 1:100 in 1% BSA/PBS with 1% AB serum. For the tertiary step, goat anti-rabbit antibody conjugated to polymer-horseradish peroxidase (DAKO, Glostrup, Denmark) was used, diluted at 1:100 in 1% BSA/PBS with 1% AB serum. Both the secondary and tertiary step required incubation for 30 min at room temperature. Next, the slides were immersed for 10 min in a solution of 0.05% 3,3′-diaminobenzidine (Sigma-Aldrich, Steinheim, Germany) and 0.03% hydrogen peroxide in PBS for visualization of the signal as brown staining. After washing with demineralized water, the slides were slightly counterstained with hematoxylin, dehydrated and mounted with Eukitt mounting medium (Sigma-Aldrich, Steinheim, Germany).
After deparaffinization, microwave antigen retrieval (700 W) was performed for 20 min in 10 mM Tris/1 mM EDTA buffer at pH 9.0, with a cool down period of 20 min afterwards. Endogenous peroxidase was blocked with 0.3% hydrogen peroxide in PBS for 20 min. Slides were incubated with a normal goat serum diluted at 1:10 in PBS for 30 min at room temperature. The primary step consisted of incubation with rabbit anti-human antibody VEGF A-20 sc-152, (Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted at 1:200 in 1% BSA/PBS for 1 h at room temperature. Only a secondary step with goat anti-rabbit antibody conjugated to polymer-horseradish peroxidase (DAKO, Glostrup, Denmark) was applied, diluted at 1:100 in 1% BSA/TBS with 1% AB serum for 30 min at room temperature. Next, the slides were immersed for 10 min in a solution of 0.05% 3,3′-diaminobenzidine (Sigma-Aldrich, Steinheim, Germany) and 0.03% hydrogen peroxide in PBS for the visualization of the signal as brown staining. After washing with demineralized water, the slides were slightly counterstained with hematoxylin, dehydrated and mounted with Eukitt mounting medium (Sigma-Aldrich, Steinheim, Germany).
3.3. Assessment of Staining Patterns
The assessment of staining patterns was performed as described before [8]. For each antigen, a pathologist (Stefano Rosati) blinded to clinical and pathological data, scored the staining intensity (0 = no staining; 1+ = weak staining; 2+ = moderate staining; 3+ = strong staining) of tumor areas for all the specimens. Specimens in which one or more tumor areas with different staining intensities were present were scored for the most prevalent intensity. Specimens with focal uptake amidst negative cancer tissue scored 1+. Furthermore, different patterns of immunoreactivity were observed and documented. To evaluate background staining, all specimens were evaluated in a field that contained both prostate cancer and stroma. Tumor distinctiveness was assessed for each antigen by subtracting the staining intensity of stroma from the staining intensity of prostate cancer.
4. Conclusions
The current study is the first to present data on the expression of PSMA, EpCAM, VEGF and GRPR in locally recurrent prostate cancer after brachytherapy or external beam radiotherapy. Based on the absence of stromal staining, PSMA, EpCAM and VEGF show high tumor distinctiveness. GRPR has a very low tumor distinctiveness. Therefore, PSMA, EpCAM and VEGF can be used as targets for the bioimaging of recurrent prostate cancer after EBRT to exclude metastatic disease and/or to plan local salvage therapy.
The Center for Translational Molecular Medicine, Project Prostate Cancer Molecular Medicine (03O-203), has financially contributed to the current research.
Conflicts of Interest
The authors declare no conflict of interest.
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|Table 1. Gleason sum scores, therapy characteristics and staining intensities of the antibodies in the salvage prostatectomy specimens.
|Patient No.
|Radiotherapy (Dose in Gy)
|Hormonal Status Prior to Salvage Prostatectomy
|Interval between Radiotherapy and Salvage Prostatectomy (Months)
|EBRT (Dose unknown)
|Brachytherapy (HDR)
|EBRT (Dose unknown)
|Brachytherapy (LDR)
|EBRT (Dose unknown)
|Brachytherapy (LDR)
|Brachytherapy (LDR)
cnd, could not be determined; EBRT, external beam radiotherapy; HDR, high dose rate; LDR, low dose rate; LHRH, luteinizing-hormone-releasing hormone agonist; AA, androgen receptor antagonist.
|Table 2. Immunohistochemical staining intensity of prostate cancer and stroma.
|Staining Intensity
|PSMA Prostate Cancer
|PSMA Stroma
|EpCAM Prostate Cancer
|EpCAM Stroma
|VEGF Prostate Cancer
|VEGF Stroma
|GRPR Prostate Cancer
|GRPR Stroma
|0 (0%)
|17 (100%)
|3 (17.7%)
|17 (100%)
|3 (17.7%)
|17 (100%)
|0 (0%)
|0 (0%)
|7 (41.2%)
|4 (23.5%)
|7 (41.2%)
|3 (17.7%)
|3 (17.7%)
|2 (11.8%)
|6 (35.3%)
|6 (35.3%)
|6 (35.3%)
|8 (47.0%)
|8 (47.0%)
|4 (23.5%)
|1 (5.8%)
|8 (47.0%)
|6 (35.3%)
|17/17 (100%)
|0/17 (0%)
|14/17 (82.3%)
|0/17 (0%)
|14/17 (82.3%)
|0/17 (0%)
|17/17 (100%)
|17/17 (100%)
|Table 3. Tumor distinctiveness.
|Tumor distinctiveness
|3 (17.7%)
|3 (17.7%)
|15 (88.2%)
|7 (41.2%)
|4 (23.5%)
|7 (41.2%)
|2 (11.8%)
|2 (11.8%)
|6 (35.3%)
|6 (35.3%)
|8 (47.0%)
|4 (23.5%)
|1 (5.8%)
Tumor distinctiveness = staining intensity tumor – staining intensity stroma.
© 2014 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).


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Things you might not realize about being in a coma until you're in one.
I was in a medically-induced coma for about a week a half in 2007. Here are some things I learned from it.
1. At least for my medically-induced coma, there is some awareness of the outside world. I incorporated the things I experienced into my very vivid dreams. When I woke up, I knew that my parents were there, I kinda knew I was in a hospital (I thought Kumail had had an accident), and I kinda knew one of my nurses. The surgical tape all over me was transformed into a guy who was chasing me with sticky candy hands. My brain found a way to assimilate, even when I couldn't.
2. The hairs on your body that you pluck meticulously? They just grow. Unfettered. Don't worry, everyone will ignore them.
3. People that work in the hospital will become friends with you while you're under, and you will have to be okay with that. I wasn't so much, I was kind of a grumpy bitch to people I thought to be strangers who had kept me from getting bedsores every day.
4. Your body will feel alien to you. Most likely, despite science's best efforts, parts of your body will swell alarmingly from water retention. Only movement makes that go away.
5. Your nails will be gorgeously, mockingly long and healthy when you wake up.
6. Kill Bill was on the right track, but still a bit off. When The Bride woke up, she could move her arms and head, but had to drag her legs behind her until she got into the Pussy Wagon and willed herself to be able to walk again. That shit is false. It's not a question of willpower- your muscles atrophy so much quicker than you think they will, and it takes weeks for them to get back to even a semblance of normal. I couldn't lift a cell phone to my head for at least a week. But maybe the Pussy Wagon has magical powers?
7. There are random local and national news stories you'll miss while you're under that will forever feel relevant to you. While I was under, a fox wandered into a cafe in Chicago and curled up in a display case. I think about that fox from time to time.
8. No one will understand the overwhelming hurricane of emotions that you will have when you wake up. No one. And that's okay, just hold on until the storm subsides a bit, and then start thinking.
9. You will always have a coma story.


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Arninge Church
Arninge Church is a Late Romanesque church in the little village of Arninge, some 8 km (5.0 mi) south of Nakskov on the Danish island of Lolland. Built of red brick in the 13th century, it has an intricately carved auricular altarpiece created by Henrik Werner in 1644.[1][2]
The church was originally dedicated to the Virgin Mary.[2]
Built of red brick, the church consists of a Romanesque apse, chancel and nave and a Gothic porch. There is a free-standing 14th century timber bell tower adjacent to the church. The chancel has traces of a round-arched south door and of a round-arched window, now bricked up. There are also traces of two Romanesque windows in the south wall of the nave above the porch. The three cross-vaults in the nave are from the Late-Romanesque period.[2]
The altarpiece (1644) was carved in the auricular style by Henrik Werner who also created the altarpiece in Maribo Cathedral. Werner's workshop also produced the carved font (c. 1640). The crucifix on the chancel wall was found on the loft during restoration work in 1937. The figure of Christ is from c. 1300 although the cross itself is more recent. The Renaissance pulpit is from c. 1605.[2][1]
See also[edit]
- ^ a b "Arninge kirke", Vestenskov.dk. (Danish) Retrieved 12 August 2013.
- ^ a b c d Kirsten Weber-Andersen, Otto Norn, Aage Roussell, Gertrud Købke Knudsen, "Arninge Kirke", Danmarks kirker: Maribo amt, Volume 8, 1951, Nationalmuseet, pages 315-327. (Danish) Retrieved 10 August 2013.
|Wikimedia Commons has media related to Arninge Kirke.
Coordinates: 54°47′26″N 11°10′19″E / 54.79056°N 11.17194°E


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|25 Feb 2013
windows 7
adobe flash player / administrator permissions
My computer was infected by a virus, so it was sent off to the retailers repair shop. When it was returned I could not use Youtube as adobe flash player was disabled, and when I tried to reinstall it, I was told I do noyt have the appropriate administrator permissions. What do I do about it. I'm knocking on a bit and don't understand computer terminology. Help, Tony
|My System Specs
|25 Feb 2013
W7 Pro SP1 64bit
Welcome to the Seven Forums!
You might want to contact that shop and ask what they did to cause this. Just write down what they say and repeat it to us here. If that is not an option - then we can take some educated guesses.
It sounds like they made your account a standard user. If you are operating as a standard user (which is a good thing), then certain actions will require information about an administrator account.
Let's test something:
Please click on the Start button (or orb)
Then right mouse click on Computer
Click on Manage from the context menu.
You might only see one account listed on the next screen:
Just click on the button named Yes without entering a password and let us know what happens.
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China Hotels Shenzhen Hotels Motel 168 Hongling - Shenzhen
Motel 168 Hongling - Shenzhen
- Location:
- Address: Hongling North Road and Nigang West Road, Futian District, Shenzhen
- Tel: 0755-26507888 (Book this hotel, call 800-2682918)
- Hotel Name in Chinese: 莫泰168 (深圳红岭店)
- Lowest Price: $22
Motel 168 Hongling has convenient transportations, with 15 minutes' driving to Luohu harbor, Huanggang harbor and 35 minutes' driving to BaoAn airport.
- Prices not include 18% service fee and tax.
- Payment Method: pay using our secure online payment service or pay at the hotel at some hotels.
- Triple/quad occupancy may require an extra bed at extra cost.
Hotel Amenities
|Hotel Services
|Car rentals, Medical clinic service, Wake Up Call, Permanent English-speaking staff, Business Centre, Copy Service, E-mail, Fax Service, Typing Service
|Room Amenities
|Air conditioning, Alarm clock, Color Television, Telephone, Bath and Shower, Internet access
Hotel Amenities: Elevator, Parking, Business Floor or Executive Floor, Air-Conditioned Facilities, Beauty Salon, Sauna, Meeting Room, Deposit Box at Front Desk, Fire Detector, Chess & Playing Cards Room
Dining Facilities: Chinese Restaurant, Western Restaurant
Print the Taxi Directions
- Please drive me to Motel 168 Hongling - Shenzhen, thank you!
- 请送我到莫泰168 (深圳红岭店),谢谢
- Address: Hongling North Road and Nigang West Road, Futian District, Shenzhen
- 地址: Hongling North Road and Nigang West Road, Futian District, Shenzhen
- Tel: 0755-26507888
- Our Reviews
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text:
Local researchers have found another foe of the honey bees: deadly parasitic flies.
Posts Tagged 'honey bee'
Leave Those Bees Alone!Wednesday, January 4th, 2012
Honey Bee HealthFriday, July 29th, 2011
What lives inside the honeybee? UC San Francisco researchers are uncovering the bee microbiome, hoping to stop Colony Collapse Disorder.
Infecting PollenMonday, December 27th, 2010
Pollen could harbor viruses– spreading the potentially deadly agents from bee to bee.
Saving the BeesThursday, October 7th, 2010
US Army researchers, and other scientists, are working hard to protect honey bees from colony collapse disorder.
Articles by Tag
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Nike LIVESTRONG Lightweight Tech Men's Running Gloves
The LIVESTRONG Lightweight Men's Running Gloves are a great way to keep your hands protected and warm During cold morning runs. The lightweight gloves feature Dri-FIT fabric to keep your hands dry and comfortable. A high-contrast pattern increases your visibility for those early morning and late night runs. 96% polyester, 2% nylon and 2% rubber.
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• Unlock access to the gear Flying Magic Carpet, Deadly Thorn Potion, Venomshank, and Telamon's xTreme Dual Shoulder Cannons
• 10 Outfit slots that save permanently
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ROBLOX is not sponsored, authorized or endorsed by any producer of plastic building bricks, including The LEGO Group, MEGA Brands, and K'Nex, and no resemblance to the products of these companies is intended. Use of this site signifies your acceptance of the Terms and Conditions.


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Michael E. Porter
Bishop William Lawrence University Professor
Harvard Business School
(617) 495-6309
Michael E. Porter is the Bishop William Lawrence University Professor at Harvard Business School. Professor Porter is a leading authority on competitive strategy; the competitiveness and economic development of nations, states, and regions; and the application of competitive principles to social problems such as health care, the environment, and corporate responsibility. Professor Porter is generally recognized as the father of the modern strategy field, and has been identified in a variety of rankings and surveys naming him the world's most influential thinker on management and competitiveness.


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Hot ringtones on Audiko.net! Here you can download a free F.R. David ringtones for your iPhone or mobile. Scope out your favorite trim from your favorite song - Words, Music, F.R-Word, or any other track. Then download the prepared F.R. David ringtones to your cell phone, and enjoy 80s music by F.R. David every time your phone rings. If you are mad with 80s or maybe Disco and france music, then you will probably like Secret Service, Gazebo, or Taco as well. For all who want a free F.R. David ringtone, we also recommend listening to Romantic, Classic Rock, and french. You are welcome to get your track piece both in the MP3 and m4a formats, compatible with both mobile phones and iPhones. Just select a track, and choose free F.R. David ringtones download option.
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For Your Ears Only: Zoo Zero are featured in the latest Von Pip Musical Express Podcast! Listen out for zero zero zeven himself, James Bond introducing! We always knew he was a fan. Click on the pic to listen!
London based avant-rock group
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See top homes from the Doory Awards.
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The World's Best Longboarders From 22 Countries Have Gathered In Historic Huanchaco, Peru, Where They Will Compete for Gold As we approach the start of the ISA World Longboard Championship, most of the participating National Delegations have announced their teams. A total of 22 countries… Read More »
ISA News
ISA World Bodyboard Championship October 12-19 Playa Parguito, Isla Margarita, Venezuela The Venezuelan Surfing Federation (FVS) and the International Surfing Association (ISA) are excited to announce the return of the ISA World Bodyboard Championship to Playa Parguito on beautiful Margarita Island, Venezuela, located in the… Read More »
From September 22-28, Peru will become the gathering place of the world's best longboarders, who will compete in the ISA World Longboard Championship (WLC) at the world-class pointbreak of Huanchacho. Over 20 countries from all corners of the planet will soon arrive to the beautiful… Read More »
The ISA President Continues To Advocate Surfing In The Olympic Movement La Jolla, California, September 10, 2013 – Thomas Bach of Germany has been elected the ninth President of the International Olympic Committee (IOC) at the 125th IOC Session in Buenos Aires, Argentina. Jacques Rogge,… Read More »
For the first time in ISA History, Longboarding Will have its Own World Championship to Take Place in Huanchaco, Trujillo, Peru, September 22nd to 28th. La Jolla, California, August 22, 2013- The International Surfing Association (ISA) is excited to announce the first stand-alone ISA World… Read More »
Also, Gary Van Wieringen (RSA) and Ross Williams (HAW) are Awarded Silver and Bronze Medals in the Masters Division of the 2011 ISA World Masters Surfing Championship ISA Headquarters, La Jolla, California, August 19, 2013- The International Surfing Association (ISA) announced today that as a… Read More »
La Jolla, California, August 12, 2013 — On Saturday night, surfing leader and ISA President Fernando Aguerre received one of the biggest honors in surfing when he was given the Waterman of the Year award, at the annual Surf Industry Manufacturers Association (SIMA) Waterman's Ball… Read More »
The ISA is Now Accepting Applications With a Deadline of August 10 The ISA is proud to continue to award academic surf scholarships to under-18 junior surfers from all over the globe. The ISA Individual Scholarship Program was established in 2007, and in its six year… Read More »
The 2013 ISA World Tandem Surfing Championship (WTSC) was held July 27 and 28, 2013 in Biscarrosse, France. Below is an interview from Surfing France with the new ISA WTSC Champions. Sarah Burel and Rico Leroy. Final Results 1. Rico Leroy and Sarah Burel (France)… Read More »
La Jolla, California, July 25, 2013- The International Surfing Association (ISA) is proud to welcome Wales as the 74th member nation of the ISA. Wales' Flag Wales, which is part of Great Britain, has a booming surfing population. Located on the western side of British… Read More »
The Tahiti Nui ISA World Kneeboard Championship crowned its champions this past Sunday July 21, in the waves of Teharu'u, Tahiti Below are the Final Results and photos of the last day of competition, along with the Awards Ceremony. Thanks Tahiti for hosting this great… Read More »
After two consecutive Lay Days, the action of the Tahiti Nui ISA World Kneeboard Championship successfully completed Day 6 of the event. Strong winds and cloudy skies dominated this day, but the waves allowed the world's best kneeboarders perform high scoring rides. Below are Day… Read More »
Bad weather and choppy conditions made the contest directors of the 2013 Tahiti Nui ISA World Kneeboard Championship call a Lay Day for Day 4 of the event. Below are the Lay Day pictures. For Day 3 Photos and Results CLICK HERE
The action continues in the 2013 Tahiti Nui ISA World Kneeboard Championship, in Teharu'u, Tahiti. Day 3 featured Tag Team competition and it crowned the first Medalists of the event. Team Venezuela won the Gold Medal, defeating Australia (Silver Medal), Tahiti (Bronze Medal), and New… Read More »
The 2013 Tahiti Nui ISA World Kneeboard Championship is underway in the fun waves of Taharu'u, Papara, Tahiti. Six nations are competing for the Team and Individual Gold Medals and the ISA Team Champion Trophy. Below are action photos and results from Day 2. Click… Read More »


text:
Posts by rob559
|Previous 1 2 3 4 5 .. 62 Next
Chartered Forex Robot In Trading Systems Oct 22 at 21:35
grid trading ,very dangerous ,don't fall into that trap inexperienced traders,trading at very high risk just to impress ang make a quick buck,stay away ,,,please
Forex Diamond EA - All Pairs - Recovery ON In Trading Systems Oct 19 at 17:37
[quote]probids posted: so reverse trading?[/quote] KIND OF,waiting for the trades to reverse
back In General Oct 16 at 19:43
we spent the last 3 months to moving in texas ,what a nice place to live,weather is wonderfull, anyone from this state here?
Happy Gold v1.2 (ThinkForex) In Trading Systems Oct 16 at 19:38
seems good but needs longer history in order to get a better analysis,at least 1 year
BlueMonkey Real Money Live Test == Ended Oct 2014 In Trading Systems Sep 18 at 15:14
s: CAUTION: August 2014: Multiple suspicious and fake reviews submitted. Related websites include BigBangEA.com and PipAble.com
Johnpaul77 In General Sep 01 at 17:39
had one acc with them fortunatly retrieve my $$ just when that failure started happening,di not lose too much
vps window 8 In General Jul 23 at 16:05
i'm experiencing issues at finding a remote desktop connection app for my laptop on window 8 ,any suggestion ?
My Scalper EA Octa Real In Trading Systems Jul 03 at 21:08
very amateurish and scam smelling this foreprofit1
BlueMonkey Real Money Live Test == Ended Oct 2014 In Trading Systems Jul 03 at 03:52
averaging down system or grid or both ,would you tell us what is it ?
My Scalper EA Octa Real In Trading Systems Jul 02 at 21:54
i think there is a lot of inexperienced people here and you think that we will fall for your pseudo system,go learn your lessons before jerking here about how your trading is " good"


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Profile: Digital River Inc (DRIV.O)
24 Oct 2014
$8.27 (+47.58%)
Digital River, Inc. (Digital River), incorporated on September 25, 1997, provides end-to-end global cloud-commerce, payments and marketing solutions to a wide variety of companies in software, consumer electronics, computer games, video games and other markets. The Company offers its clients a broad range of services that enable them to quickly and cost effectively establish an online sales channel capability and to subsequently manage and grow online sales on a global basis while mitigating risks. The Company is engaged in providing outsourced commerce solutions globally to a variety of companies, primarily in the software and consumer electronics product markets.
The Company's services include design, development and hosting of online stores and shopping carts, store merchandising and optimization, order management, denied parties screening, export controls and management, tax compliance and management, fraud management, digital product delivery via download, physical product fulfillment, subscription management, online marketing including e-mail marketing, management of affiliate programs, paid search programs, payment processing services, Website optimization, Web analytics and reporting, and CD production and delivery.
The Company's products and services allow the Company's clients to focus on promoting and marketing their products and brands worldwide while leveraging its investments in technology and infrastructure to facilitate the purchase of products through their online websites. The Company provides access and uses of its platforms to its clients as a service as opposed to selling the software to be operated on their own in-house computer hardware. The Company's cloud-commerce store solutions range from simple remote control models to more comprehensive online store models.
In addition to the services the Company provides that facilitate the completion of an online transaction, the Company also offers services designed to increase traffic to its clients' websites and the associated online stores and to improve the sales productivity of those stores. The Company's services include paid search advertising, search engine optimization, affiliate marketing, store optimization, multi-variant testing, Web analytic services and e-mail optimization. All of the Company's services are designed to help its clients acquire customers more effectively, sell to those customers more often and more efficiently, and increase the lifetime value of each customer.Additionally, through the Company's Digital River World Payments subsidiary, the Company offers a full range of payment processing services to clients. These services include multiple payment methods, fraud management, tax management, cloud-based billing and other payment optimization services.
Store Design, Development and Hosting
The Company offers its clients Website design services utilizing its experience and to create efficient and effective online stores. The Company's global-commerce solutions can be deployed quickly and implemented in a variety of ways from fully-functioning shopping carts through completely merchandised online stores. The online stores the Company operates for its clients often matches their branding and Website design to provide a seamless experience for shoppers. The Company manages the order process through payment processing, fraud screening and fulfillment (either digital or physical) and notifies the buyer via e-mail once the transaction is completed. Transaction information is captured and stored in the Company's database systems, an increasingly valuable source of information used to create targeted merchandising programs, e-mail marketing campaigns, product offers and test marketing programs.
For many of the Company's clients, the solution the Company provides is critical to their businesses and therefore the Company operates global data centers that perform and scales for continuous e-commerce operation in a high-demand environment. The Company operates multiple data centers globally, which feature fully redundant high-speed connections to the Internet, server capacity to handle unpredictable spikes in traffic and transactions, 24/7 security and monitoring, back-up electric generators and dedicated power supplies.
Store Merchandising
The Company's technology platforms support a wide range of merchandising activities. This enables the Company's clients to effectively execute promotions, up-sell, and cross-sell activities and to feature specific products and services during any phase of the shopping process. The Company's clients' online stores through the checkout and thank you pages, its solution allows clients to deliver targeted offers designed to increase order close ratios and average order sizes.
Order Management and Fraud Screening
The Company manages all phases of a shopper's order on its clients' online stores. The Company processes payment transactions for orders placed through its technology platform and support a wide variety of payment types, including credit cards, wire transfers, purchase orders, money orders, direct debit cards and many other payment methods in the United States and around the world. The fraud screening component of the Company's platform uses both rules-based and heuristic scoring methods which use observations of known fraudulent activities to make a determination regarding the validity of the order, buyer and payment information. The Company also provides denied-parties screening and exports controls, which are designed to ensure that persons and/or organizations appearing on government denied-parties lists are blocked from making purchases through its system.
Digital and Physical Fulfillment Services
The Company provides both digital and physical fulfillment services to its clients. The Company offers its clients a broad array of electronic delivery capabilities that enable delivery of digital products directly to customers' computers via the Internet. the Company's technology platform and then securely downloaded to the purchaser. Optionally, buyers can, for an additional fee, request that a CD be created and shipped as a backup for their order.
In addition to electronic fulfillment via download, the Company offers physical distribution services to its clients as well. The Company has contracted with third-party fulfillment agents that maintain inventories of physical products for shipment to buyers. These products are held by the fulfillment agent on consignment from the Company's clients. The Company provides notification of product shipment to the buyer as well as shipment tracking, order status and inventory information. The Company also provides a service called Physical on Demand (POD), which utilizes robotic systems to create a client-branded product CD and packaging materials after a POD order has been placed. The Company provides extended download services for digital products for additional fees, which enables buyers to download the products they have purchased more than once in the event of a computer failure or other unexpected problem. The Company offers management services relating to regulatory matters such as the Waste Electronics and Electrical Equipment laws.
Customer Service
The Company's client's option and for an additional fee, the Company provides telephone and e-mail customer support for products sold through its platforms. The Company provides assistance to buyers regarding ordering and delivery questions on a 24/7 basis in multiple languages. The Company continues to invest in technology and infrastructure to provides fast and efficient responses to customer inquiries as well as provide online self-help options.
Advanced Reporting and Analytics
The Company captures and store detailed information about visitor traffics for sales in the online stores the Company manages for its clients. The Company provides clients access to a collection of standard and customizable reports via its Web analytics technology. This enables the Company's clients to track and analyze sales, products, transactions, customer behavior and the results of marketing campaigns so they can optimize their marketing efforts to increase traffic, order close ratios and average order values.
Strategic Marketing Services
The Company offers a range of strategic marketing services designed to customer acquisition, improve customer retention and enhance the lifetime value of each customer. Through a combination of Web analytics, analytics-based statistical testing, optimization and direct marketing practices. The Company delivers and manages programs, such as paid search advertising, search engine optimization, affiliate marketing, store optimization and e-mail optimization on behalf of its clients. The Company can precisely determine the effectiveness of specific marketing activities, Website changes, and other actions taken by its clients.
Payment Services
The Company offers full service payment provider solutions for online merchants around the world. The Company connects businesses to the local payment methods that their customers prefer and support businesses to expands into new markets through enabling the acceptance and processing of a diverse range of payment methods and options. The Company offers a broad range of back office payment reconciliation services that result in a cost efficient and secure program for e-payments.
The Company sells and market these services through a direct sales force and channels partners located around the world, primarily in the United States and Europe. The Company's technology platform provides for high transaction throughput in a secure and data sensitive environment. These services are provided either via a direct interface between the client's commerce system and the Company's payment services platform or via a Payment Card Industry (PCI) compliant wrapped secure Web based payment page that is served to the client's commerce system on a transaction by transaction basis.
The Company competes with Oracle Corporation; IBM Corporation; Demandware, Inc.; hybris GmbH; IBM Global Services; Accenture, Inc.; AG; eBay, Inc.; Avangate BV; asknet AG; arvato Systems; WorldPay Ltd.;GlobalCollect; CyberSource Corporation; Square, Inc.; PayPal Corporation; ValueClick, Inc.; Microsoft Advertising; Amazon.com, Inc.; Buy.com, Inc.; Network Solutions, LLC; Akamai Technologies, Inc.;Yahoo!,Inc.; eBay, Inc., and Hostopia.com, Inc.
Company Address
Digital River Inc
10380 Bren Road West
Company Web Links
Thomas Madison
David Dobson
Stefan Schulz
Theodore Cahall
Thomas Peterson
- Well-timed bullish option bets seen in Digital River weeks before deal
- Well-timed bullish option bets seen in Digital River weeks before deal
- Digital River to be acquired by investor group for $840 million
- Digital River to be acquired by investor group for $840 mln


text:
By Jef With One F
The 10 Biggest Rockers Living in Texas
By Rocks Off
By Chris Lane
By Angelica Leicht
By Corey Deiterman
Drake Returns to Warehouse Live
By Brando
By Angelica Leicht
6 Bands No One Noticed Broke Up
By Corey Deiterman
2706 White Oak
Houston, TX 77007
Category: Music Venues
Region: Heights
Scale the Summit is well on its way to becoming the second instrumental prog-rock band from Texas to really take off. Much like Austin's Explosions in the Sky, which earned the ears of countless fans and critics on the Friday Night Lights soundtrack, this Houston-based four-piece has received acclaim from almost everyone who has taken the time to listen to their tunes, including critics at Revolver, Guitar World and the Houston Chronicle. The band also has toured with Dream Theater, from whom it draws much of its influence. Scale the Summit formed in 2004, self-released its debut album Monument in 2007 and was subsequently signed to Prosthetic Records. Since then, it has released two more albums, including last year's The Collective. Originally, the band wasn't supposed to be an instrumental-only project, but by the time guitarists Chris Letchford and Travis Levrier found a bass player and a drummer, the music they had been writing sounded like it was complete, so they kept it that way.


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A Good Day to Die Hard
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DVD release of the 2013 motion picture.
In English, dubbed French or dubbed Spanish, with optional subtitles in English, Spanish or French.
Video recordings for people with visual disabilities.
Video recordings for the hearing impaired.
Feature films.
Action and adventure films.
Feature films.
Fathers and sons
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OK action flick. Really bad plot though.
I've watched all the Die Hards & enjoyed most but this one was ridiculously far fetched. It was laughable. I can only assume that is the last in the series as they have clearly run out of ideas.
Lots of shooting and explosions. Not much rationale.
DVD is badly scratched and will not play all the way through.
Waaaay too many cut aways. I was dizzy trying to keep up with the pacing. Not that it mattered since they could run into a cement wall and come out with only dirt stains. Sad when all actor can say during a car chase is "Jesus" and "F***"! And Whoever did the music should be taken out to desert and left; horrible music that took away any good feelings I had toward this movie.
This show is fine, so long you don't that it seriously, or look for much of a plot. It's like the others in the series of Die Hard movies - predictable. But, I had fun viewing it, wondering how many cars were wrecked in the big chase scene.
Typical action flick. Light on substance, big on explosions and elaborate chase scenes. If that's your cup of tea, you'll love this.
hyperbolic car wrecks and if you can suspend belief and accept that these guys are bulletproof you might enjoy this. It would help if you are 12 years old or younger.
The old Die Hards were subtle compared to this.
very good
Some very good action and Bruce is good except when he is being fed stupid lines, which is unfortunately too often. The humor is stupid, the writing is mostly stupid and there are some serious pacing problems, but the positives are strong enough to make this enjoyable, while you shake your head wishing they had done much of this differently. Especially if you are a Die Hard fan.
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A Good Day to Die Hard trailer


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by Tummeow
Stationery Cards
Set of folded stationery cards printed on bright white, smooth card stock to bring your personal artistic style to everyday correspondence. Each card is blank on the inside and includes a soft white, European fold envelope for mailing.
- 238Promote
Many thanks for your lovely comments Megstuff, Diego Verhagen, Roger Wedegis, Fabian Gonzalez, Michael Creese, Karol Livote, Carina Povarchik, plearn and Goosi, I really appreciate. :)


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By Brian Deakyne
RUMSON – Thirty seconds.
That's the amount of time it took to decide the fate of the Rumson-Fair Haven Regional High School boys' soccer team's season on Friday, Nov. 15, during the NJSIAA, Central Jersey, Group 2 sectional final.
In the 42nd minute, Rumson's Matt Dell nearly


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Wow, the portions are so liberal.Nazar15107 Addison Rd.Addison972-726-0100Hardly anything has changed since this place transformed itself from the Turkish restaurant called Shish to the Turkish restaurant called Nazar.Oh, there's a new owner and all. But I'm almost certain I had the same waiter a ... More >>
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Chris McQuarrie won an Oscar for Usual Suspects, to which Hollywood responded, 'Big deal'
Digging up the facts behind Egyptian excavations


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Okay, now I'm upset. You see, I love music. I love hip-hop. And I used to try my hand at producing, so I love to trying to taking seemingly impossible songs to sample and trying to sample them. (That sentence was oddly constructed, yeah?) Anyway, one of those seemingly impossible songs to sample, in my opinion, is Otis Redding's "Try A Little Tenderness." If you're old like me, you probably associate the song with Duckie Dale's performance in the middle of the record shop in Pretty in Pink. (Shout out to Emily Rugburn, ha!) Or maybe you remember it from the "Fleet Week" episode of Sex and The City, when Carrie walks down Broadway toward Times Square and the title card comes up paying tribute to NYC right after 9/11.
Or maybe you're just ignorant and have never heard the song. Which is a shame.
In any case, Kanye West—and I have to believe it was Kanye on the production tip—has done the impossible. He sampled "Try A Little Tenderness." And of course, it is bangin'. The track, aptly titled "Otis," is off Watch the Throne, Ye's upcoming collabo album with his big brother Jay-Z. It's a monster track, with both MCs kicking smart-ass verses and taking veiled shots at folks who (maybe? perhaps?) took veiled shots at them.
Damn, yet another oddly constructed sentence. I'll get it together at some point.
What do you think of "Otis"? After the torturous "H.A.M.," which was released earlier this year, does this raise your expectations for Watch The Throne? LMK below.
Watch The Throne releases on August 1 digitally and August 5 on CD, vinyl, cassette, and such. Shout to the Radar for the track.


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Andy demonstrates bowl making, on the potters wheel at the Western Washington Fairgrounds, in the Artists In Action area of the Puyallup County Fair. The bowls were then donated to the Tacoma Metro Parks for their annual Empty Bowls fundraiser.
Eating Eggs
We will no longer be selling at the Port Orchard Farmers Market. If you need eggs you are welcome to come out to our farm to pick them up. Please call first to make sure we are home. 253 973-8068
Fernwood Creamery also carries our eggs and... you can pick up some of their yummy raw milk while you are there. http://www.fernwoodcreamery.com/
Raincreek Pottery and Poultry is a studio pottery and chicken farm located near the town of Port Orchard in a rural section of Kitsap County in the Pacific Northwest State of Washington. The Kitsap Peninsula is due west of Seattle across Puget Sound. Raincreek produces functional pottery, dinnerware, garden markers, jewelery, and pet related items from Stoneware and Porcelain clay bodies fired to cone 10 (2350 F) in a propane fueled kiln. Owned and operated by Andy and Pam Buck, Raincreek Pottery was established in 1991. Our chicken hatchery operations began in early 2010.
Raincreek Pottery and Poultry's pottery products can be found at the Verksted Gallery in Poulsbo, Washington, and both pottery and poultry are currently offered, during the summer months, at the Port Orchard Farmers Market.


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Perhaps we're guided by an illusion; we're surely not guided by consciousness. --------------------------------------------------------------
A blog dedicated to all the uplands - from the humble hills of Denmark to the lofty peaks of Nepal.
The alternative peak and me.
Rocks, Skin, and Sun on Behance
little sth i did last month on the seaside


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Open for lunch and dinner daily except Sundays. The best place to experience salad in Steinbeck Country - the Salad Bowl Capital of the World.
Delivery available to businesses in Salinas and surrounding communities.
(Minimum order required. Please call us for details)
This is a sampling of our menu items. Additionally, we offer a variety of side salads, chips, desserts and beverages. Please call us for more information or to inquire on daily soup specials. Feeding a small or large gathering... visit our catering page, we can help.