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Product Reviews
Sunday loves: What's new in beauty
We give you a round up of the latest and greatest in new beauty products.
This week we're obsessed with
A glorious milk drink, Kate on diets, Benedict and penguins, Parisian street style and much, much more ...
Sunday loves: Hyaluronic acid moisturisers
Here's what we're loving this weekend, when it comes to special face creams.
On trial: Elizabeth Arden's Five-Minute Facial
If you ignore the initial tattoo-like sensation (promise it's worth it), this new duo will sort out your pores in no time.
Sunday loves: New beauty arrivals
We take a look at what's new and luscious in makeup and skincare.
Getting the good oil on skincare
Oils that promise better skin aren't of the snake variety any more.
Bath + mask = bliss
Is there anything more restorative than a steaming hot bath? Try throwing in a spa-worthy face mask too.
Could this be the source of perfect skin?
Serums are the potent, powerful, rock stars of skincare.
Sunday loves: eye creams
We have gathered together three creams that help the appearance of the delicate skin around the eyes.
This week we're obsessed with
James Franco and Peter Dinklage's new hairdos, a fresh chocolate flavour, stylish raincoats and some scary drag.
Straight hair & chip free nails at home
We're in love with two new products that mean silky hair and chip free nails without a salon price tag.
Face scrubs are back
From fine powders to electrical gadgets... here's all you need to know about facial scrubs.
Join us to trial Lancôme's new Advanced Skin Corrector
5 great beauty buys for under $10
These beauty scores are cheap as chips but come with better bells and whistles than their pricier counterparts.
Beauty: After glow
Anti-pigmentation products may be just the thing to get you closer to complexion perfection.
Beauty trend: Marvellous matte
Glossy lips be gone, the pendulum is swinging back the other way as matte textures come back into fashion.
Splurge or steal? CC Creams
The latest skin perfecting product is taking over. So should you fork out or stick to a budget?
Celebrity hair products you should try
Sure, they've got damn good locks - but should you actually give Katie, Jen and Claudia's haircare brands a go?
How to nail this season's hottest shades
You might not be ready for winter clothes, but the latest polish shades are an easy autumn accessory to try.
Pencil-case beauty tools
These wee gems are straight from the stationery cupboard: think lip crayons, eye markers and even hair chalk.
On trial: Nioxin Diaboost hair thickener
We put the latest hair thickening product to the test, because who doesn't want fuller, bouncier tresses?
Automated curling tongs - which is best?
Moving your arm to curl your hair - so outdated! These days the new hair tools take care of the motion for you.
Great or gimmick? Spray-on moisturiser
A body lotion that claims to nourish your skin without leaving your hands greasy... Does it really work?
This hair product will change your life
Fancy spending less at the salon and living without horror roots? Well get excited ...
Power up your shower
If you're merely using your shower time to wash, you're most definitely missing out...
This week we're obsessed with ...
Supermodels and selfies, the best shampoo ever and a sex bomb of a figure skating routine.
Great or gimmick? Scented nails
Are polishes with a lovely scent the next big thing or just a flash in the pan? We review them honestly.
The cheap secret to perfect falsies
The funny little tool that makes power-lash application all the easier.
This week we're obsessed with...
Miley and Justin's morph, Lena Dunham's awesomeness and new beauty & fashion finds.
Five sunscreens with extras
These slip-slop-slap gems can also make you smell better or look more bronzed ...
5 Anti-ageing creams under $50
Sick of shelling out the mega-bucks for anti-wrinkle products? Here are five cheaper creams you can trust.
Star buy: Dry conditioners
Just when we've got a handle on dry shampoos, here comes a whole new clever beauty category.
Splurge or steal?: Organic oils
Should you spend up large on organic facial oils or can you bag a bargain?
Review: Revlon Grow Luscious Lash Potion
Need to work some magic on your lashes? This mascara has a wand to work miracles.
Splurge or steal?: Bee venom
If you want to really 'bee' beautiful, bee venom is the way to go. But should you spend up large on it?
Cheap chic: Garnier Ambre Solaire No Streaks Bronzer Self-Tanning Mousse
Afraid that cheap stuff will turn you into a streaky mess? Fear not, this one is fab, and you can win some!
Splurge or steal?: Shower gels
Should you pay big bucks for a shower gel or is that akin to washing money down the plughole?
Show us your jazz hands
Add some sparkle and razzamatazz with these six glam glitter enamels.
Cheap chic: Dominate Out of Bed Hair Shaper
Lads, not a fan of sticky, spiky hair? We've found the perfect hair wax product for you.
Beauty product of the week: L'Oreal Paris NutriLift Gold Anti-Ageing Serum Foundation
Foundations come and go but this one is a real sparkler. It actually contains tiny real gold particles.
Cheap chic: Aveeno Positively Radiant Moisturiser
We think this is one of the best value-for-money products on the market - and it costs just over $20.
Splurge or Steal? Feet treats
We pit a budget option against a luxurious beauty buy to see which will lead to happy feet.
Five fab new celebrity fragrances
Want to smell like a star? Then check out these five hot new celeb fragrances - and you might win one!
Five of the best: Illuminators
Five great skin highlighters that will add shimmer to your style.
Cheap chic: Nivea In-Shower Body Lotion
We have found the ultimate time-saving, value for money beauty product - and you can win some!
Ten great fake tans to try this season
Forget your fake tan fears - here's how to glow without the sun, without the smell and without the stress.
Splurge or steal: Yellow nails
Yellow is a cool summer shade for nails, but should you spend up large or go for cheap and cheerful?
In our beauty bags: August 2013
This month we're loving an eyeliner that stays on all day, a Jersey-Shore-proof bronzer and much more ...
Review: Clinique Dramatically Different Moisturising Lotion+
Clinique have launched a reformulated take on their famous yellow moisturiser, and you can win some!
In our beauty bags: July 2013
Our new finds include a cheap gloss-lipstick hybrid, soap for your hair and natural deodorant that works.
See her amazing makeup transformations
When a neighbour feeds your cat
The 10 couples we wish were still together
Seven saviours for the oily skinned
When the school photoshops your six-year-old
Is this the golden formula of marriage?
Is it us or retailers obsessed with body size?
Loro Piana suits the finest money can't buy
Sutton: Running reduces stress
John Key reminds Aucklanders of property risks
Ribs are normal, not a sign of anorexia
NZ daily street style: October '14
See her amazing makeup transformations
8 things I learned after giving up processed foods
John Key reminds Aucklanders of property risks
'Health' foods to leave behind
When a neighbour feeds your cat
The 10 couples we wish were still together
Your towels are dirtier than you think
Aussies urged to lose grabbable guts


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|Manufacturer: Danze
Model #:D403221
List Price:405.00
Danze is a manufacturer of high quality decorative plumbing products for your powder room, kitchen and bath. Danze offers a broad variety of products, designs and finishes to suit your own particular needs. This D403221 Antioch Single Handle Kitchen Sink Faucet, with Spray, finished in Chrome will provide years of beauty and trouble-free performance. There is meticulous attention to detail on every piece of this faucet.

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The Rider's International Contoured Diamond Quilt Dressage Pad is shaped to provide wither relief and a secure fit under your saddle. This classically-styled dressage pad features attractive 3/4" mini-diamond quilting, a durable cotton top, a soft flannel lining and foam/poly fill for cushioning. 26" x 46".
The Roma® Ecole Double Diamond Dressage Pad makes your horse comfortable with its cushioned micro trico wicking lining and contoured topline. This elegant dressage pad features subtle colors, double diamond quilting and two-tone piping . A hidden girth guard protects the edge of the pad for durability.
Liven things up with the Roma® Ecole Flower Diamond Dressage Pad in polished cotton. The pad is quilted in an appealing pattern of flowers and diamonds and richly trimmed in gold or silver.
The Rider's International Large Diamond Pad is perfect for schooling. This all-purpose saddle pad features striking diamond-shaped quilting and is contoured for a comfortable fit. 23" x 41".
The Rider's International Wave Pad features a gentle wave quilting pattern and double braiding. This contoured all-purpose saddle pad is made of durable cotton over a fluffy layer of poly fill. 23" x 41".


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Home » NewHampshire.com » Travel & Tourism
Brother's death shakes Bode Miller, family
"We still live in a 'village,'" Starcy Branch, a friend of the family's said Tuesday, noting there is an outpouring of love for Joanne Kenney Miller and Woody Miller of Easton, parents of the 29-year-old who died Sunday of an apparent seizure.
Miller was hoping to make the U.S. squad for snowboardcross for the 2014 winter Olympic games in Sochi, Russia. He'd returned to the sport after recovering from a 2005 motorcycle accident that left him in a coma for 11 days. He came in fourth at the 2013 Sprint U.S. Snowboarding Grand Prix. In March he won the Rahlves Banzai Tour overall title.
He was found in his van in the Mammoth Lakes area of California.
Miller grew up in Easton, as did his brother, and learned to ski as soon as he could walk. He discovered snowboarding when he was about 9, and immediately fell in love with it. He was sweet and loving and very close to his family, Branch said.
"He just liked people," she said. "It's very, very tragic. He really made an impact because he was so kind."
Branch called him, "a massively great snowboarder," who also excelled at other outdoor sports.
"We all know everyone," she said of the Easton-Sugar Hill-Franconia area. Everyone, she added, is uniting to help the family. "Their hearts are broken."
John DeVivo, general manager of Cannon Mountain, said Chelone moved out west before DeVivo came to Cannon six years ago.
"Everybody who knew him says he was a great kid, with a lot of spirit. Everyone seems to have a story or two from back in their youth," DeVivo said.
Bode Miller was at Cannon last weekend, raising money for the family's Turtle Ridge Foundation at BodeFest. His sister, Kyla Miller works at the foundation in Sugar Hill; another sister, Wren Miller Weed, is on the board of directors.
"Our thoughts and prayers are with the family," DeVivo said.
"Chelone Miller was an aspiring elite athlete who had made great progress as a snowboardcross rider this past season. We are all deeply saddened at the news of his death and extend our condolences to the entire Miller family," said Bill Marolt, president and CEO of the U.S. Ski and Snowboard Association.
|NH Angle >> Human Interest
0 Video of reporter vs. Keene Pumpkin Festival organizer goes viral
0 NH police dog Daisy Mae headed to Pennsylvania to hunt cop killer
0 Parents give son's killer home and job
0 Donovan the osprey reaches South America
1 Man who bet it all and lost on Apple supplier writes heartbreaking letter
0 80 immigrants from 39 countries become NH citizens
0 Destruction of memorial adds pain to woman's loss: Boyfriend was shot to death in May
0 Beliefs divide lesbian, Catholic family
0 Globe: Did Fremont just have its first homicide ever?
0 Video: NH Indian man goes to India to study film making, discovers 'I was not Indian enough for India'
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Mail Account Wizard 0.1
Create mail accounts easily.
Mail Account Wizard is an attempt to make a better wizard to create mail accounts. Most of the times, we use the same settings for IMAP and SMTP. Sometimes very few things are different. This wizard tries to integrate both send/receive accounts, as well as the identity to use and folder settings.
- last updated on:
- January 13th, 2009, 10:48 GMT
- price:
- developed by:
- Miguel Tadeu
- homepage:
- www.kde-apps.org
- license type:
- GPL (GNU General Public License)
- category:
- ROOT \ Communications \ Email
In a hurry? Add it to your Download Basket!
Add your review!


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Milwaukee Brewers Carlos Gomez, Arizona Diamondbacks Gerardo Parr...Doug Mittler
In ESPN The Magazine's Money Issue, Doug Mittler writes that as new defensive metrics gain relevance in MLB, bargain players like Carlos Gomez and Gerardo Parra won't be underpaid for long.Story | Conversation | April 16, 2014
MLB division previews based on formula for clubhouse chemistry --...Jeff Phillips
For ESPN The Magazine's MLB Preview Issue, experts built a fancy algorithm that measures clubhouse chemistry. The result? The Rays should ready themselves for lots of Champagne showers come October.Story | Conversation | March 20, 2014
MLB - Future Power Rankings 2014Jim Bowden, Keith Law and Buster Olney
Jim Bowden, Keith Law and Buster Olney bring you the latest installment of the Future Power Rankings, looking at how well each team is set up for sustained success.Story | Conversation | March 10, 2014
Los Angeles Dodgers come out on top of peak value rankings - MLBJared Cross
Jared Cross ranks all 30 teams assuming every player is at his peak, with the Dodgers coming out on top.Story | Conversation | February 14, 2014
Allen Webster and D.J. Peterson among prospects who just missed t...Keith Law
Keith Law breaks down 10 prospects who just missed his top 100 rankings.Story | Conversation | January 31, 2014
Top 100 prospects of 2014 including Byron Buxton, Xander Bogaerts...Keith Law
Keith Law ranks the top 100 prospects of 2014, starting with Twins outfielder Byron Buxton.Story | Conversation | January 27, 2014
Houston Astros top farm system rankings - MLBKeith Law
Keith Law ranks all 30 farm systems, with the Astros coming out on top.Story | Conversation | January 26, 2014
Byron Buxton tops 2014 ranking of top 100 prospects - MLBKeith Law
Keith Law ranks the top 100 prospects of 2014, starting with Twins outfielder Byron Buxton.Story | Conversation | December 19, 2013
Robinson Cano tops this year's free-agent rankings - MLBKeith Law
Keith Law ranks the top 50 free agents in this winter's class, starting with Robinson Cano.Story | Conversation | October 22, 2013
MLB Future Power Rankings 2013 updateJim Bowden, Keith Law & Buster Olney
Keith Law, Jim Bowden and Buster Olney rank each team based on how well they are set up for sustained success.Story | Conversation | August 29, 2013
Byron Buxton of the Minnesota Twins is the new No. 1 prospect in ...Keith Law
Keith Law updates his ranking of the top prospects in baseball.Story | Conversation | July 16, 2013
Mark Appel back at No. 1 to Houston Astros in final mock draft - ...Keith Law
Keith Law unveils his final mock draft for 2013, and word is that the Astros are making a run at Stanford's Mark Appel.Story | Conversation | June 06, 2013
Prep right-hander Phil Bickford jumps into top 10 in latest mock ...Keith Law
Keith Law unveils his second mock draft for 2013, and the Astros are being linked to a new name at No. 1 overall.Story | Conversation | June 03, 2013
Houston Astros take Colin Moran over Mark Appel in second mock dr...Keith Law
Keith Law unveils his second mock draft for 2013, and the Astros are being linked to a new name at No. 1 overall.Story | Conversation | May 29, 2013
Detroit Tigers among the three teams to emerge as a cut above the...Paul Swydan
Paul Swydan breaks down the chances for the Detroit Tigers and Texas Rangers to remain elite throughout the season. He also writes why the Red Sox have struggled as of late.Story | Conversation | May 13, 2013
Projecting the National League standings for 2013 - MLBDan Szymborski
Dan Szymborski projects the NL standings, including each team's odds of making the playoffs and winning the World Series.Story | Conversation | March 26, 2013


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Portland St. Vikings (14-13)@
Sacramento St. Hornets (9-18)
Thursday, February 23
Hornets Nest
Attendance: 1,132
Portland St.
Sacramento St.
Game Leaders
38 pts, 69 fg%, 92 ft%
26 pts, 52 fg%, 100 ft%
5 reb
5 reb
7 reb
5 ast
13 ast
Sacramento, CA (Sports Network) - Charles Odum scored a career-high 38 points as the Portland State Vikings held on to defeat the Sacramento State Hornets 94-88 in Big Sky Conference action at The Nest.
Odum shot 11-of-16 from the floor, 5-of-8 from the three-point line, and 11- of-12 from the foul line for Portland State (14-13, 8-6 Big Sky). Renado Parker put up 19 points, Lateef McMullan netted 14, and Chehales Tapscott added 11 for the Vikings, who have won four of five.
For Sacramento State (9-18, 4-11), John Dickson scored 26 points while Jackson Carbajal and Dylan Garrity tallied 18 apiece. Garrity also dished out 13 assists and Joe Eberhard chipped in 12 points for the Hornets, who have lost four in a row.
Both teams shot just over 48 percent from the field in the first half and Portland State took a slim 38-37 lead into the break.
The teams spent most of the first part of the second stanza on the sea saw. After Sacramento State took a 69-67 lead on an Eberhard three-pointer with 8:16 left, Portland State went on a 15-3 run to take an 82-72 advantage with just under four minutes remaining. The Hornets would get no closer than five points of the lead in the final minutes.
Portland State shot 53.6 percent for the game and Sacramento State wasn't far behind at 50.8 percent.
Jimmy Casas, Daniel Acosta, Dennis Flannery Jr.


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- Shopping Bag ( 0 items )
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Posted December 20, 2012
With Fire and Sword!
The book itself is amazing!! But this version of it is a disgrace to its kind!! Its all mushy and the translation is messy. There are lot of typos and symbols. Plus there are slightly cleaned up versions for free on the nook. I own the trilogy and all in hardcover. It may be difficult to get your hands on such books, but i would recomend it because its not at all better or worthwhile for that matter in the ebooks.Was this review helpful? Yes NoThank you for your feedback. Report this reviewThank you, this review has been flagged.
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Find commute times to your work, school, gym and more.
Lovely 4/3 2 Story Home in Alafaya Woods!
1041 Covington St, Oviedo FL 32765 0 Bed
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Lovely 4/3 2 Story Home in Alafaya Woods!
Must see this lovely 4 bedroom, 3 bath home in excellent condition! Balcony off Master Bedroom. Mexican Tile in Kitchen, Tile in living room, Wood floor in dining room and Berber Carpet throughout! Community Playground and Tennis Courts!
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YooSocial - Social Bookmarks Plugin
Description du produit
YooSocial is a Joomla content plugin that enables your users to bookmark Joomla articles over social networks. You can enable/disbale sharing on a social network basis and exclude article categories from being shared.
- Available for Joomla 1.5, 2.5 and 3.x.
- YooSocial for Joomla 3 is responsive
- You can exclude some categories in plugin settings to not display social icons on articles you do not want to share
- Display on top/display on bottom settings to enable sharing without having to edit your Joomla articles
Version 3.0.0


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5 awesome pie pairings to try now!
This guy is a-maze-ingly sweet
My room is a disaster zone. Help!
The biggest OMG celeb moments of the week
Feel-Good Friday: Little Willow's costume craze is super cute
Sporty chic: Cool workout clothes you can actually wear beyond the gym
Amp up your costume with these cute Halloween makeup DIYs
Break a sweat: Try this surprisingly fun hula hoop exercise
Are you social media obsessed?
Ever feel a little jealous? Spill for an upcoming issue of the mag!
Kirby Buckets Clip
More Friends = More Fun
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12 HOURS AGO Can girls make the first move—or is that weird? http://t.co/tgtlB9KNYl
15 HOURS AGO #DailyGiveaway! 1 Western chick wrangles these @BuccoCapensis navy boots: http://t.co/o8FUIQYMbr
15 HOURS AGO Snag a sneak peek of @jacquieleemusic's new EP: http://t.co/CsAj7j0goN #thevoice
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Try something new
Whether you've had your eye on that adorbs DIY project or you've been itching to pick up a cool sport, go for it! Knock out feeling lonely and feeling bored with something fresh and new to do. When fall rolls around, you'll be psyched to show off your latest skills.
Reach out
When it comes to friends, the more the merrier. Your BGF might be at soccer camp and your bestie may be on her annual family vacay, but that doesn't mean you can't find some new buds to chill with! There's absolutely no reason to feel like your cheating on your pals- they want you to have fun, and branching out could lead to a surprisingly awesome new friendship!
Turn parents into pals
You'll be surprised by how cool the 'rents can be if you give 'em a chance. So spend a day in the sun with mom, dad, or both and learn how much fun you can have with fam. Plus, they'll be touched that their super sweet daughter put aside some time to hang with them.
Focus on yourself
Use this summer to refresh yourself, both inside and out. Make a quick list of qualities you'd love to work on, like patience, generosity, and kindness. Then make a plan to do a little better each day. To complete the new you, try out the latest beauty trends, rearrange your room, or do whatever else you can to give your life an instant perk-up.
How are you making new pals this summer? Tell us in the comments!
POSTED IN how can I make new friends, dealing with jealousy, I'm feeling left out, afraid of being alone, boredom buster
6 books to curl up with this season...
It'll be one fierce fall when you pick up our six picks from the bold new batch of Fierce Reads. CLICK HERE to check out the titles, score free chapters and more!


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News from WTOP
News from the AP
Mike Causey
Shows & Panels
Shows & Panels
- The 2014 Big Picture on Cyber Security
- AFCEA Answers
- American Readiness: Renewable Power and Efficiency Technologies
- Ask the CIO
- Building the Hybrid Cloud
- CDWG/NetApp
- Connected Government: How to Build and Procure Network Services for the Future
- Continuing Diagnostics and Mitigation: Discussion of Progress and Next Steps
- Delivering the Digital Government Mission
- Federal Executive Forum
- Federal News Radio's National Cyber Security Awareness Month Special Panel Discussion
- Federal Tech Talk
- The Future of Government Data Centers
- The Future of IT: How CIOs Can Enable the Service-Oriented Enterprise
- Government Perspectives on Mobility and the Cloud
- The Intersection: Where Technology Meets Transformation
- Maximizing ROI Through Data Center Consolidation
- Mitigating Insider Threats in Virtual & Cloud Environments
- Modern Mission Critical Series
- The New Generation of Database
- Reimagining the Next Generation of Government
- Targeting Advanced Threats: Proven Methods from Detection through Remediation
- Transformative Technology: Desktop Virtualization in Government
- The Truth About IT Opex and Software Defined Networking
- Air Traffic Management Transformation Report
- Cloud First Report
- General Dynamics IT Enterprise Center
- Gov Cloud Minute
- Government in Technology Series
- Homeland Security Cybersecurity Market Report
- National Cybersecurity Awareness Month
- Technology Insights
- The Cyber Security Report
- The Next Generation Cyber Security Experts
Shows & Panels
What's new with BRAC?
Saturday - 1/14/2012, 1:25am EST
January 13, 2012 -- Host Derrick Dortch gets a BRAC update from Dr. Andrea Morris, BRAC Coordinator for Arlington County, Virginia.
She will talk about reductions in federal workforce due to BRAC, and what the BRAC Transition Center is doing to help laid off government workers.


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Video Of The Week - The Best 'Let It Go' Parody Ever?
With Frozen's catchy song 'Let it go' winning the Oscar last night, there are bound to be numerous more parodies added to the hundreds of thousands that already exist on YouTube. However, very few will be more fun or relevant (given the recent winter storms) than the one performed on Friday, February 21st, by Bob Herzog on Cincinnati, Ohio's WKRC.
Dubbed 'Just Don't Go', the traffic reporter sung his dramatic parody against a backdrop of cars skidding on ice and himself, trudging and slipping on the snow. With over 1.7 million views and counting, it will be probably go down as the most watched traffic update in history!
This of course is not the first time the veteran reporter has performed a parody for the station's Dance Party Friday segments. His past covers include Lorde's 'Royals' that begins with 'I've never seen a day without a crash' and Elton John's 'Rocket Man' that he transformed to 'Traffic Man'. Who knew watching a traffic report could be so much fun?
Resources: popwatch.ew.com,
- What did the Bob Herzog do?
- What was he trying to convey in a fun way?
- Do you think his message got across?
Why do people create parodies of their favorite songs?
608 Comments
- xxbaconloverxxThursday, October 16, 2014 at 7:56 ambest parody of frozen ever!lololololololololololololololololol
- DJ 1004Thursday, October 9, 2014 at 1:21 pmLOL LOL
- neko2457Tuesday, October 7, 2014 at 3:38 pmxD OMG LOL Did he just make up that song?? Amazing how people can make such fitting parodies!
- coolgurlz44321Saturday, October 4, 2014 at 7:41 am):I MAKE MORE VIDS!
- coolgurlz44321Saturday, October 4, 2014 at 7:40 amOMG THIS TRAFFIC VID IS HILARIOUS!!!!!
- Girlonfire112Friday, September 26, 2014 at 12:37 pmThe cold never bother him anyway
- rainbowstar1230Thursday, September 25, 2014 at 6:02 pmLOL
- smartguy20Saturday, September 20, 2014 at 2:53 pmLOL
- doglover2079Tuesday, September 9, 2014 at 5:33 pm#Crazyweather
- doglover2079Tuesday, September 9, 2014 at 5:33 pmGood voice
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The awesome smiley emoticon!
This great custom product in purple from our department Women and T-Shirt category can be printed just for you in the size and style you like. The design Awesome Smiley was created by KimmieShops and uploaded to Spreadshirt for you to have printed on a T-Shirt. Next to a host of other great customized designs, you can find this Awesome Smiley design in Spreadshirt's Satire category. If you are interested in topics like Face or Fun, then you'll love Awesome Smiley designs by KimmieShops. Spreadshirt also offers a huge selection of custom t-shirts, hoodies and accessories for the category Satire as well as top Happiness clothing. KimmieShops's designs have already impressed many of our customers, so we are sure that we've got right stuff for you! Spreadshirt is your destination for great designs of all kinds – whether it's Face, Fun or Happiness. We are sure that you will find something nice in our shop, there are many amazing custom Satire t-shirts, hoodies, or accessories for you to find!Tags: Design ID: 11312956
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The Top 10 In Action Sports
01Champagne, Cabaret And Raving With The Pope: We Meet The Man Behind The Craziest Après Bar In The Alps...
02Don't Look Down! 8 Sky-High Glass Floors That People With Vertigo Should Avoid…
03France Vs. Austria: Which is Better for Skiing & Snowboarding?
04WARNING: These Incredible Photos of #VanLife Will Make You Want to Jack In Your Job
05Bad-Mouthing, Board Stabbing and Full On Fist Fights in the Water: These Are the Greatest Rivalries Surfing Has Ever Seen
0610 Terrifying Pistes Every True Skiier Needs To Ride Before They Die
07No Waves? No Problem. This Guy Can Turn Any Beach Into A Surfing Nirvana. He Just Needs £50,000...
08This Documentary About Illegal Longboard Racing in Hawaii is Strangely Compelling...
09She Was Attacked By A Stranger Outside Her House, Now Steph Gilmore Has Conquered Her Fears But Will She Claim Her Sixth World Title?
10This Company Will Turn You Into Snow After You Die... And Then Let You Fall On The Mountains
Cory Lopez - Legendary
A Surfing video by surfeurope - 7,518 views
At 35 years old Cory Lopez is in the best shape of his life and not slowing down. Recently, Cory took the groms down to Costa and showed them how real men work. He was up at 4:30 each morning doing his wake up rounds, in the water by 5:30. Cory was ripping whatever surf he could find for 10 hours a day, eating two meals, just pure stoke and love for surfing. In Costa Rica he is known as Legendary and this video will show you why...
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Home prices on the rise
POSTED: Wednesday, August 26, 2009 - 5:32pm
UPDATED: Wednesday, March 17, 2010 - 12:05pm
WASHINGTON (AP) - Sales of new homes jumped 9.6 percent in July, providing yet another sign the housing market is climbing back from the lows seen early this year. Helped by falling prices, the fourth-straight monthly increase was stronger than expected.
The Commerce Department says sales rose to a seasonally adjusted annual rate of 433,000. June's sales were revised higher. Sales have now risen more than 30 percent from the bottom in January, but are still off nearly 70 percent from the frenzied peak four years ago.
The median sales price was down slightly from June and off 11.5 percent from year-ago levels. Prices, however, have risen from March's low.
Homebuyers are rushing to take advantage of a federal tax credit that covers 10 percent of the home price, or up to $8,000, for first-time owners. Home sales must be completed by the end of November for buyers to qualify.
Builders and real estate agents are pressing Congress for that credit to be extended. If it isn't, there are fears that sales could reverse the upward trend.


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2012 328i Sport Canadian Version
View Single Post
New Member
Drives: 2012 F30 328i
Join Date: Sep 2009
Location: Toronto, Ontario
Posts: 21
iTrader: (
regarding missing features, when I ordered my 2007 e90 (323) it was missing heated mirrors and heated washer jets. when I notified the dealer they said those options were not evan available on the car. they showed me a brochure which confirmed what they were saying.
knowing that all Canadian BMWs have always been equipped with 'cold climate' features, I went back to the brochure that the dealer originally gave me. surprise, surprise, my version said the car came with heated mirrors (it didn't say anything about the jets specifically but i was able to find out that they were supposed to be bundled together).
The service department said too bad. BMW Canada was of no use. BBB and OMVIC were just as helpful. So I wrote a letter to the owner of the dealership (and labelled it as private and confidential to ensure no one else would open it). Within a couple of days I got a call from the service department to bring my car in to do a retrofit of the missing equipment at no charge.
many companies do this, here's an article from the toronto star regarding a lexus ct200h
i think it's crazy that car companies can get away with this kind of stuff by putting a catch all disclaimer saying that what you order may not be what you get. if dell did that with computers, or sony did that with tv's, they'd have a pr nightmare on their hands.
oh, sorry, the core i7 pc you ordered with windows 7 was not available so we shipped you a core i5 with windows vista... close enough!
Last edited by bobby79; 04-23-2012 at
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(910): View more from North Carolina
Although I feel like awkward kinda describes your entire sexual history...
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Although I feel like awkward kinda describes your entire sexual history...
Just got a blowjob from a coed in exchange for saving her an iPhone 5 when I get them in stock. Sometimes it's awesome to be a Verizon employee.
There is not greater feeling than lying to your boss and leaving work to shit in the comfort of your own home
Guess who used an inflatable mattress to boat across a retention pond with brooms for oars and a radio and beer.
I gasped. Both pairs of lips did.
The guy who was The Count on Sesame Street died this week too. Therefore, you should take multiple shots, count them, & go "ahh aaahh aaaahhh" after each one. I expect video...
No, we got so into acting out our role play characters we didn't even fuck. still sucess.
before the moonshine you were already braiding the bouncers beard -_-
So what's today's forecast for the female rollercoaster you've been riding?
A man that refers to my vagina in third person is a man after my own heart.
I've got a permanent seat at the "Girls who eat their feelings" table this weekend.
He came when Ron Burgundy started playing the jazz flute. How do you think it went?
I told him if he went to see magic mike with me I'd cover his eyes during the penis parts
Also I'm sitting home alone with a big ass bowl of marshmallows right now just eating. It's so sad.
Really? Uh ohh sounds like a double date with extra stripper funnnn


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The stakes climb higher!
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North America treated to partial solar eclipse today
North Americans, get set for the fourth and final eclipse of the year.
North Americans, get set for the fourth and final eclipse of the year.
Using a calligraphy style he has worked to develop for more than a decade, Don Wakamatsu takes manager Ned Yost's lineup and writes out the …
Texas Health Presbyterian Hospital Dallas said in financial statements Wednesday that its revenue fell 25 percent in the first 20 days of Oc…
Topeka police say the latest accident occurred Wednesday night when the motorcycle and another vehicle collided.
Stephen Schneider and his wife, Linda Schneider, were convicted in 2010.
Wichita police are investigating a shooting in the 800 block of South Belmont. It happened around 5:50 a.m. Thursday morning.
Experts say college can be overwhelming for first-generation students, filled with unfamiliar jargon and procedures.
Rain ending then mild weather returns.
Robert is wanted by the Sedgwick County Sheriff's Office for A PROBATION VIOLATION FOR POSSESSION OF NARCOTIC DRUGS.
In the words of a fellow newsroom worker, "This was a much more fun game than last night!"


text:
Town Square
Sort by: Most recent topic/comment Most viewed Most comments
Navigation tip: Clicking on any category label will display all recent posts in that category
PALO ALTO ISSUESStanford Blood Center lawsuit on hold
A jury trial pitting a longtime blood donor against Stanford Blood Center that was scheduled to begin today has been postponed,...Original post made on Sep. 24, 2012
Last comment by Nevemore, on Oct 16, 2014 at 8:44 pm | 26 comments | 2537 Views
PALO ALTO ISSUESDifficult conversation: when to give up the car keys
Louise Freedman recently carried her driver's license into the Department of Motor Vehicles (DMV) and laid it on the counter. Read...Original post made on Oct. 13, 2014
Last comment by Disabled But Not Dead, on Oct 16, 2014 at 8:40 pm | 10 comments | 3766 Views
PALO ALTO ISSUES40 Hour Work Week for City Employees
Do any of the candidates running for city council support the normal concept of city employees working 40 hours per week?...Original post made by Barron Park resident on Oct. 15, 2014
Last comment by Ha ha, on Oct 16, 2014 at 8:23 pm | 4 comments | 215 Views
PALO ALTO ISSUESBuena Vista residents appeal closure decision
Seeking to stave off displacement, residents of Buena Vista Mobile Home Park are appealing a September decision by a hearing...Original post made on Oct. 15, 2014
Last comment by John B, on Oct 16, 2014 at 6:33 pm | 45 comments | 4957 Views
ISSUES BEYOND PALO ALTOJudges On the Ballott
I'm not familiar with most of the names on the ballot. Can someone refer me to a good article and/or opine here. Thanks...Original post made by Online Name on Oct. 16, 2014
There are no comments yet. 62 Views
PALO ALTO ISSUESResidential Permit Parking Enforcement
The City of Palo Alto has issued a Request for Proposal (RFP) for a consultant to provide Residential Preferential Parking...Original post made by Herb Borock on Oct. 10, 2014
Last comment by Herb Borock, on Oct 16, 2014 at 1:56 pm | 3 comments | 192 Views
PALO ALTO ISSUESPalo Alto to ponder its lobbying priorities
Faced with pressure to build more housing, improve flood protection and plan for high-speed rail, Palo Alto officials will...Original post made on Oct. 14, 2014
Last comment by resident 1, on Oct 16, 2014 at 12:24 pm | 11 comments | 1041 Views
PALO ALTO ISSUESStudy: Caltrain trench could cost $1 billion
Digging a trench for Caltrain in the southern half of Palo Alto could cost as much as $1 billion, according to a study that...Original post made on Oct. 10, 2014
Last comment by Its only $1 Bil?!?, on Oct 16, 2014 at 11:16 am | 80 comments | 4549 Views
CRIMES & INCIDENTSCaltrain reports pedestrian fatality near Charleston
Caltrain confirmed on Wednesday evening at 6 p.m. that a pedestrian fatality had occurred near Charleston Avenue on the Caltrain...Original post made on Oct. 15, 2014
Last comment by Rev. Daniel, on Oct 16, 2014 at 10:47 am | 3 comments | 15703 Views
PALO ALTO ISSUESWith no rain in sight, Adobe Creek undercrossing to stay open
As California's drought persists, Palo Alto is preparing to leave open an undercrossing at Adobe Creek that normally gets...Original post made on Oct. 13, 2014
Last comment by The Shadow knows....., on Oct 16, 2014 at 9:14 am | 17 comments | 2780 Views
SCHOOLS & KIDSParents Nursery School Carnival, Sunday 10/19
Looking for something fun to do with your kids on Sunday, October 19!!? Search no further!!! Come to Parents Nursery School's...Original post made by Parents Nursery School on Oct. 15, 2014
There are no comments yet. 99 Views
PALO ALTO ISSUESCaltrain horn
Can you hear the Caltrains horn? I'm working to reduce the noise of the Caltrain at the level crossing at Charleston...Original post made by Oliver Sisson on Oct. 3, 2014
Last comment by Stan, on Oct 15, 2014 at 8:25 pm | 11 comments | 422 Views
PALO ALTO ISSUESMitchell Park Library project nears finish line
Palo Alto's tortuous path toward rebuilding its largest library hit a significant milestone last week when the new Mitchell...Original post made on Oct. 14, 2014
Last comment by midtown library user, on Oct 15, 2014 at 7:37 pm | 18 comments | 2926 Views
SCHOOLS & KIDSHelping others rather than asking for Halloween candy this Trick or Treat time
What about Unicef or collecting canned food for foodbanks? Do our kids really need to get more candy this Halloween? They...Original post made by Parent on Oct. 15, 2014
There are no comments yet. 85 Views
CRIMES & INCIDENTSPalo Alto: Pedestrian killed in suspected DUI crash identified
A pedestrian killed on Monday morning in a collision with a suspected DUI driver on East Bayshore Road in Palo Alto has been...Original post made on Oct. 14, 2014
Last comment by Jasmine, on Oct 14, 2014 at 11:54 pm | 6 comments | 4801 Views
SCHOOLS & KIDSPalo Alto school board to discuss achievement gap committee
Superintendent Max McGee's call for an achievement gap task force made up of students, teachers and community members will...Original post made on Oct. 14, 2014
Last comment by musical, on Oct 14, 2014 at 8:27 pm | 2 comments | 797 Views
AROUND TOWNCalifornia Avenue work now set to stretch until March
Palo Alto's reconstruction of California Avenue, which officials had expected to complete by the end of this year, is now...Original post made on Aug. 20, 2014
Last comment by neighbor, on Oct 14, 2014 at 5:55 pm | 33 comments | 5330 Views
ISSUES BEYOND PALO ALTOWoodside teen dies by suicide on Interstate 280
The California Highway Patrol is reporting the death at about 12:45 p.m. on Tuesday, Oct. 14, of a 17-year-old Woodside girl...Original post made on Oct. 14, 2014
There are no comments yet. 38767 Views
CRIMES & INCIDENTSMan posing as IT worker burglarizes Peninsula office buildings
Police are searching for a man suspected in a series of office burglaries in Palo Alto, Burlingame and San Mateo and who...Original post made on Oct. 14, 2014
Last comment by Chris, on Oct 14, 2014 at 2:56 pm | 3 comments | 2934 Views
PALO ALTO ISSUESMug shots and mental illness
How does one's beloved child become a mug shot in the local newspaper? This is our somewhat abbreviated story about the ravages...Original post made on Sep. 12, 2014
Last comment by Jean, on Oct 14, 2014 at 2:31 pm | 46 comments | 5639 Views
Page 7.25 of 1042
Best High Dives to Watch the Game
By Laura Stec | 14 comments | 2,535 views
By Chandrama Anderson | 1 comment | 1,410 views
King of the Slides
By Cheryl Bac | 2 comments | 1,095 views
The Future of our Parks: Public Workshops this Week
By Cathy Kirkman | 0 comments | 540 views
NO MEAT ATHLETE Workout/Running Group
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So my hunt for natural concealer may have come to an end. Mineral Fusion is an all natural make-up company that creates glorious hypoallergenic and paraben free ingredients! YESSSS! They are 100% vegetarian and most of their products are also VEGAN. It is void of artificial colours, fragrances, gluten, talc, and phthalates - helping ALL skin types flourish! It also doesn't have that shimmery effect right between your eyes that most concealers have, so you won't be a disco ball in pictures.
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Charging problems
"Purchase Cards: Increased Management and Oversight Could Save Hundreds of Millions of Dollars"
Members of Congress are asking government agency buyers, "What's in your wallet?" as they consider proposed legislation to limit the use of purchase cards.
The legislation, introduced in April, would require agency officials to seek discounts for purchases made with the
cards, and to use contracts or other procurement methods to save as much money as possible.
Rep. Jan Schakowsky (D-Ill.) introduced the bill in the House, and Sen. Susan Collins (R-Maine) sponsored a similar one in the Senate. The bills also would require agencies to collect and analyze data on the cards' use.
The legislation comes in response to an April General Accounting Office report that shows how increased management and oversight of purchase cards could save the government hundreds of millions
of dollars, according to a spokesman in Schakowsky's office. That report follows others that found a litany of problems with agency purchase card programs, specifically at the Navy.
"How many more hearings must Congress have on GAO reports before taking real action to bring about financial accountability in the federal government?" Schakowsky asked in a written statement.
The cards, which look like credit cards, are intended to streamline bill processing, thereby all but eliminating the need for paper purchase orders. Agency employees bought about $1 billion in goods and services using the cards when they were first introduced in 1994.
By 2003, the cards were used to buy
$16.4 billion worth of goods and services, according to GAO's report.
The report also states that agencies have left themselves vulnerable to fraudulent, improper and abusive purchase card use through poor management. Earlier reports, for example, found that the cards were used to buy many personal things such as high-end luxury items and holiday gifts.
The new report states that agency officials generally do not take advantage of opportunities to get better prices from vendors.
The six federal departments that GAO officials examined —
Agriculture, Defense, Interior, Justice, Transportation and Veterans Affairs — could save a total of $300 million a year if they received discounts of 10 percent from vendors that earn more than $1 million a year from purchase card buys. Those six agencies account for more than
85 percent of purchase card use, according to GAO's report.
Procurement experts said that additional discounts might be difficult to arrange. Vendors on the General Services Administration's schedule contracts are already supposed to include a discount when agency employees use the cards, attorney Jonathan Aronie said.
"You don't have the concern at a place like Home Depot or Office Depot, which both have negotiated discounts," he said. "But if you walk into your local hardware store, the government's paying retail."
That might be a necessary consequence of owning purchase cards, Aronie said. "Part of the reason we have purchase cards is so people can run out [for emergency purchases], and in some cases, the government needs to pay retail — unless we want our government officials to drive an hour to save $2," he said.
The purchase card program has grown impressively, said Alan Chvotkin, senior vice president and counsel for the Professional Services Council, a trade group representing service firms.
"It has morphed over time into a substantial acquisition technique," he said. "But one of the concerns is that the government is not taking advantage of the volume purchasing it can do, particularly with repetitive vendors."
Chvotkin cautioned against the proposed legislation's possible effect on small businesses. If the law requires agencies to negotiate more discounts, officials might find that easier to deal with larger firms.
"It's hard to negotiate with 20 or 30 distributors collectively," he said.
The bills suggest that agencies could negotiate volume discounts, but high-volume purchases are less likely to come through small firms, Chvotkin said.
"We do that as consumer shoppers as well," he said. "That's why places like Price Club and Sam's Club do so well. But if you only need one tube of toothpaste, you go to the convenience store and you pay more."
Larry Allen, executive vice president of the Coalition for Government Procurement, argued that purchase cards were not meant to eliminate the need to look for good values.
"As a practical matter, purchase cards are just supposed to be tools," he said. "Agencies aren't supposed to use purchase cards with disregard for procurement rules. The rules still say that you're supposed to get a good deal for the government, but I think that gets overlooked."
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A key contributor to Clinger-Cohen argues that federal IT has failed to take advantage of the tools that law provided.
A new report surveys the many different approaches to launching a dedicated innovation shop in government.
Nominations for 2015 are being accepted now through Dec. 23.
The flip side of default data encryption on mobile devices is that a lost or stolen device won't yield up its secrets -- an important feature for many federal employees.
A decade after the 9/11 Commission identified agencies' reluctance to share information as a dangerous shortcoming, the government is moving in the right direction.
The latest offering is intended to help agencies speed the process of deploying authorized cloud solutions.
Cartoonist John Klossner on the telltale indicators that not everything is running through the CIO.
SBA's innovation office invests in solutions to problems that don't yet exist – like the 2007 seed funding for research into an effective Ebola treatment.
The government-wide IT contract's final awards were put off a month as NASA waded through 233 proposals.
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Northern District of Alabama Torts - Injury Cases
Cases filed
Cases 1 - 10 of 5,676
Ashburn v. Robinson et al
as 4:2014cv02044
Plaintiff: Tammy Underwood Ashburn
Defendant: Jeremy Robinson, First Coast Logistics Services, Inc.
Cause Of Action: Diversity-Tort/Motor Vehicle (P.I.)
Type: Torts - Injury > Motor Vehicle
Stanley v. Winn Dixie Stores Inc
as 2:2014cv02032
Plaintiff: Matthew Stanley
Defendant: Winn Dixie Montgomery LLC
Cause Of Action: Petition for Removal- Personal Injury
Newell v. Abbvie Inc et al
as 2:2014cv02034
Plaintiff: Robert Wayne Newell
Defendant: Abbvie Inc, Abbott Laboratories Inc
Cause Of Action: Diversity-Product Liability
Walker v. Pfizer Inc
as 2:2014cv02033
Plaintiff: Kenneth W Walker, Jr.
Defendant: Pfizer Inc
Cause Of Action: Diversity-Personal Injury
Johnson et al v. Logans Roadhouse Inc
as 5:2014cv02037
Plaintiff: Loretta Johnson , Ray Johnson
Defendant: Logans Roadhouse Inc
Cause Of Action: Petition for Removal- Personal Injury
Grace v. Winn-Dixie Montgomery LLC et al
as 6:2014cv01977
Plaintiff: Candice Grace
Defendant: Winn-Dixie Montgomery LLC , Jeff Shook
Cause Of Action: Petition for Removal- Personal Injury
Type: Torts - Injury > P.I.: Other
Clark v. Planned Parenthood Southeast, Inc. et al
as 2:2014cv01939
Plaintiff: Roberta Clark
Defendant: Planned Parenthood Southeast, Inc. , Planned Parenthood of Georgia, Planned Parenthood of Alabama and others
Cause Of Action: Diversity-Medical Malpractice
Alabama Concrete Co., Inc. et al v. Argos Cement, LLC et al
as 5:2014cv01940
Plaintiff: Alabama Concrete Co., Inc. , Mary Jane Mitchell , Mitchell Roland
Defendant: Argos Cement, LLC , L. Bruce Ables
Cause Of Action: Petition for Removal- Personal Injury
Type: Torts - Injury > P.I.: Other
Beal et al v. DePuy Orthapedics Inc et al
as 5:2014cv01922
Plaintiff: Roger Beal , Debra Beal
Defendant: DePuy Orthapedics Inc, Depuy International Limited, Depuy Inc and others
Cause Of Action: Diversity-Personal Injury
Parker v. Logan's Roadhouse Inc
as 5:2014cv01926
Defendant: Logan's Roadhouse Inc
Plaintiff: Gina Parker
Cause Of Action: Petition for Removal- Personal Injury
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Search: MatrixSearch profiles »
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History of Molecular Genetics and Biotechnology Help (page 3)
History of Molecular Genetics and Biotechnology
Prior to discovery that the chemical nature of the gene was DNA, in the early 1900s the gene was an abstract unit of heredity. This period in history is referred to as classical or formal genetics. Classical genetics was extremely successful in elucidatingmany basic biological principles without understanding the physical nature of the gene. The era of molecular genetics began with the discovery of DNA structure in 1953. In the late 1970s, yet another milestone in genetics was reached when researchers found that they could manipulate DNA molecules in a test tube (in vitro), essentially "cloning" the first gene. This discovery led to further molecular genetic advances including recombinant DNA technology (genetic engineering) and spawned the biotechnology industry known today.
The histories of most scientific disciplines are generally characterized by relatively long periods of stagnation punctuated by bursts of rapid progress. Most of these flurries of research are initiated by new technical developments. This is certainly true of biochemistry and molecular biology. At least three major areas of technology have been influential in this respect: (1) instrumentation and techniques, (2) radioactive and fluorescent labels, and (3) nucleic acid enzymology. Beyond these technological influences, the use of computer technology to process biological information (i.e., DNA nucleotide sequences or protein amino acid sequences) is driving new discoveries in molecular genetics and biotechnology. In fact, several new fields within molecular genetics—Bioinformatics, Genomics, and Proteomics—have been created. Each of these fields integrates the use of computer hardware and software to store, process and analyze biological information, such as DNA or protein sequences.
The Analytical Ultracentrifuge
The analytical ultracentrifuge was developed in the 1920s by Theodor Svedberg. The sedimentation rate of a substance during ultracentrifugation is mainly a function of its density and secondarily of its shape. The unit of sedimentation (S, in honor of Svedberg) is an expression of these parameters. The centrifuge has been modified for isolating organelles such as nuclei, ribosomes, mitochondria, and chloroplasts. It can be used for determining the minimum number of kinds of macromolecules in a biological specimen and for estimating the molecular weights of macromolecules.
The Electron Microscope
The electron microscope was invented in the 1930s, and eventually enabled the direct visualization not only of cellular substructures but also of viruses and macromolecules. Circular genetic maps of microorganisms have been shown by electron microscopy to have a corresponding circular physical structure. Multiple ribosomes attached to an mRNA molecule (polysomes) have also been visualized by this instrument.
Electrophoresis is a technique that separates molecules according to their shapes, net charges, and molecular weights in an electric field, usually on solid or semisolid support media such as paper or agarose. This technique was first used in 1949 to differentiate sickle-cell hemoglobin from normal hemoglobin. Protein sequence analysis later revealed that the difference in electrophoretic mobilities of proteins was due to a single amino acid change in the β-chains. Electrophoresis is now used in a variety of DNA and protein analysis applications. Agarose gels are usually employed to isolate and estimate the size of DNA fragments, and DNA sequencing results are obtained using similar gel matrices. Proteins are often analyzed for their size and charge using polyacrylamide gels. Electrophoresis has been extensively used to differentiate isozymes, i.e., proteins possessing the same enzymatic properties but differing in primary structure. Techniques have also been developed for two-dimensional gel electrophoresis (2-D) to further separate mixtures of proteins that might be of similar sizes (or mass) but have slightly different charges (or isoelectric points). These types of gels are first run in one dimensions using size as the separator then, using pH (an indicator of charge), proteins are separated from one another at a 90 degree angle to the size dimension. Complex images of protein "spots" are created using this technique. Researchers can physically isolate the protein from the gel matrix and subject it to mass spectrometry to determine the sequence of some of the amino acids within the spot.
X-ray-diffraction data from crystalline materials has been critical for the elucidation of three-dimensional shapes of nucleic acids (e.g., DNA, tRNAs) and proteins (e.g., myoglobins, viral capsomeres, enzymes). This technique has been expanded to include the determination of multi-subunit molecules.
During the mid-1940s and early 1950s, various forms of chromatography were perfected, enabling molecules to be separated by differences in solubilities in organic solvents, electrical charge, molecular weight, and specific binding properties for the support medium, or combinations of these factors. Erwin Chargaff used paper chromatography to determine the base compositions of DNAs from various sources. He found that the molecular ratio of adenine was equivalent to that of thymine and the ratio of guanine equals that of cytosine. This was a vital clue for James Watson and Francis Crick in their search for the structure of DNA.
Automated equipment is now available for doing many repetitive biochemical tasks. DNA synthesizers can be programmed to make oligonucleotide (many nucleotides) sequences of any desired composition. Automated instrumentation is now available for isolating DNA, and sequencing DNA or protein fragments, as well as for performing enzyme assays and other tasks. Computer software has been developed to interpret data from electropherograms, and to search databases for similar or identical sequences (bioinformatics).
Several techniques have been developed to separate, rejoin, synthesize, or break nucleic acid molecules. Separation of the complementary chains of a DNA molecule is known as denaturation. DNA is denatured if placed in alkali (0.2N NaOH) or when boiled. The latter process is referred to as melting. Separation of DNA strands can be detected by spectrophotometric instruments; optical density (OD) or absorbance at 260 nm increases during the melting process. The temperature at which the increase in OD260 is 50% of that attained when strand separation is complete is known as the melting temperature (Tm). Because G and C base-pair by three hydrogen bonds, whereas A and T base-pair by two hydrogen bonds, the higher the G-C content in DNA the higher the melting temperature. Melting is enhanced where there are clusters of A's and T's, and also when all the purines (A, G) are on one strand and all the pyrimidines (T, C) are on the other strand.
If DNA is boiled and then quickly cooled, the strands will remain single; if cooled slowly, complementary strands will base-pair and reform double-helical DNA molecules. This process is called renaturation or annealing. Hybrid DNA-RNA molecules can be produced by analogous processes from single strands. RNA can be totally hydrolyzed to nucleotides by exposure to high pH (alkali). This property can be used to purify DNA from amixture of DNA and RNA. Single-stranded DNA will bind to membranous filters made of nitrocellulose; RNA will pass through such filters. However, if single-stranded RNA is complementary to nitrocellulose-bound single strands of DNA, it will form DNA-RNA hybrid molecules and be retained by such a filter. This technique is used in various blotting procedures, such as Southern, Northern and Western blotting (see Section 7 later in this chapter).
There are two main methods for breaking long DNA molecules into fragments of suitable size for sequencing or for recombinant DNA techniques: (1) mechanical shearing and (2) restriction end onuclease treatment. If a solution of DNA is subjected to the stirring forces of a Waring blender or forced through a narrow tube, the ends of long DNA strands will usually move at different speeds; this stretches the DNA and tends to break it. This phenomenon is called shearing. The higher the stirring speed, or velocity, of flow through an orifice, the greater the shearing force. Shearing can also be achieved by exposure of a solution of DNA to ultrasound. The effectiveness of any shearing force increases with molecular size of the DNA, but decreases with concentration because entanglement of DNA molecules reduces the effective stretching. Restriction endonuclease treatment employs the use of enzymes that break the bonds between specific base-pair sequences of DNA. These enzymes will be discussed later in this chapter in the sections Nucleic Acid Enzymology, and Recombinant DNA Technology.
Radioactive Labels
Radioactive elements have historically been used as highly sensitive labels for detecting minute amounts of specific macromolecules. However, recent advances in fluorescent molecule technology (i.e., the development of fluorescent dyes to tag molecules of interest) have led to the replacement of many radioactive tracers by these fluorescent molecules. Fluorescent molecules are often safer to use, easier to dispose of and better for the environment.
EXAMPLE 12.1 A. D. Hershey and M. Chase differentially labeled the nucleic acid and the protein components of T2 phages. They used radioactive 32P in place of normal 32P to label DNA; radioactive 35S was used in place of normal 35S to label protein (cysteine and methionine are two amino acids that contain sulfur). Since there is no phosphorus in phage proteins and no sulfur in nucleic acids, the fate of both viral components could be followed during the viral life cycle. After allowing the phages to become attached to sensitive Escherichia coli host cells, the mixture was subjected to the shearing forces of a Waring blender. The mixture was centrifuged to sediment the cells and then activity characteristic of each radionuclide was assayed in the pellet and in the supernatant fluid. All of the 32P activity was found in the bacterial pellet and virtually all of the 35S was found in the supernatant. 32P was found in some progeny phages, but no 35S was found. The inference is that phages inject their DNA into host cells. Blender treatment shears the phage tail fibers from receptor sites on host cells; the empty phage protein capsids (ghosts) are therefore left free in the supernate. Semiconservative replication from the infecting 32P-labeled DNA caused some progeny phages to be released with one of the original radioactively labeled infecting strands. This experiment was the first to demonstrate that DNA, and not protein, is the genetic material in phages.
DNA labeled with radioactive elements can be revealed in a photographic technique called autoradiography. A preparation of DNA on a filter paper can be covered with a photographic film. As the radioactive elements undergo radioactive decay, they release charged particles that cause a chemical reaction on the film. After development of the X-ray film, the location of the labeled DNA is revealed by dark spots.
A radioactive isotope of phosphorus (32P) is also widely used to label nucleic acids; it emits a strong beta particle and has a half-life of 14.3 days. An instrument called a scintillation counter is used to detect radioactive disintegrations.
Any organic substance can be labeled with radioactive carbon (14C). This isotope emits a weak beta particle and has a relatively long half-life of 5730 years. All living organisms incorporate a predictable amount of 14C while alive. After death, 14C decays to 14N at the predictable rate of its half-life. This knowledge allows the dating of organic remains from the time of death up to about 40,000 years before the present.
Radioactive iodine (125I) has a half-life of about 60 days, emits γ-rays, and is used to label proteins. Highenergy γ-rays (gamma) can be detected by a crystal scintillation counter. A liquid scintillation counter must be used to detect weaker beta particles, although it can also detect γ-rays. This isotope is easily coupled to the amino acid tyrosine. Radioactive sulfur (35S) is used to label the amino acids cysteine and methionine; 35S can also be used to label nucleic acids. 35S is more desirable than 32P for most autoradiography because it has a half-life of 87.1 days and emits a much weaker beta particle that gives sharper bands. It is much less hazardous to handle than 32P and poses less waste-disposal problems.
Even nonradioactive isotopes (e.g., 15N) have been useful in solving fundamental problems in molecular biology.
Fluorescent Labels
Fluorescent molecules contain a fluorophore that absorbs and emits light at particular wavelengths characteristic to the specific fluorophore. This emitted light can be detected and recorded by a number of different instruments (microscopes, scanners, spectrophotometers, etc). Fluorescent molecules (probes) can be attached to a variety of biological molecules in order to detect and study them. DNA, proteins, and lipids, as well as specific molecules, such as actin and tubulin, can be labeled with a fluorescent molecule. It is possible to use two or more fluorescent probes in one experiment if their emitted light occurs at different wavelengths. For example, different molecules within a cell can each be labeled with a different fluorescent molecule and the activities and amounts of each molecule can be detected and compared at the same time. During DNA sequencing reactions, each nucleotide is labeled with a different fluorescent molecule, allowing strands terminating with a different nucleotide to be differentiated yet detected simultaneously (see section on DNA Sequencing later in this chapter).
Nucleic Acid Enzymology
Nucleases are enzymes that hydrolyze, or break, the phosphodiester bonds that hold the nucleotides together. Those that remove terminal nucleotides one at a time are called exonucleases; those that break the sugar-phosphate backbone at nonterminal sites are called endonucleases.Adeoxyribonuclease (DNase) degrades DNA molecules; a ribonuclease (RNase) degrades RNA molecules. Some endonucleases act nonspecifically, cleaving the phosphodiester bonds at different unspecified nucleotide sequences. Others, such as the restriction endonucleases, break the bonds only at specific DNA sequences, called recognition sites. There are three classes of restriction endonucleases: Types I, II and III. Types I and III do not have qualities useful for recombinant DNA technology (i.e., they cut DNA at random sites). Type II enzymes recognize and bind to a specific double-stranded DNA sequence. Once bound, they cleave the phosphodiester backbone of each strand at or near (within 20 bp) the site. The recognition sites of these enzymes are symmetrical and commonly consist of 4–8 bp that are either continuous (e.g.,GAATTC) or interrupted (e.g., CANTG, where N is any base). The symmetry occurs around a midpoint, or axis of symmetry, formed on opposite DNA strands by inverted base sequences called palindromes. These enzymes occur naturally in bacteria, and hundreds of enzymes have been isolated and characterized and are available for use by genetic engineers. Thus, Type II enzymes are the most commonly used in recombinant DNA technology today.
Restriction enzymes play a role in defending bacteria against invasion by foreign nucleic acids, such as viruses. The host bacterial cell restriction enzyme systems can recognize the invading DNA as foreign and destroy it (see Example 11.1). Host DNA may be protected by specific base-pair modifications carried out by host-specific methylase enzymes. Thus, the restriction enzyme works in conjunction with the modifying enzyme and together the system is referred to a restriction-modification system. Foreign DNA is recognized as such because its restriction sites are unmodified. Restriction endonucleases are named after the bacterial species or strain from which they were derived. For example, an enzyme from Providentia stuartii, will have the name Pst derived from the first letter of the genus (P) and the first two letters of the specific epithet (st). This name is italicized to honor the scientific name of the bacterium and can be followed by roman numerals to indicate that it is one of several enzymes isolated from that particular bacterial strain, e.g. PstI, PstII (the numerals are not italicized). Occasionally, a letter derived from the specific bacterial strain follows the name. For example, the enzyme EcoRI was derived from E. coli strain RY13 and Hind III was derived from Haemophilus influenzae strain Rd. If this is the case neither the strain letter nor the numerals are italicized.
EXAMPLE 12.2 The EcoRI restriction enzyme cuts bonds on the upper and lower strands within the palindromic DNA sequence at the arrows shown below.
Notice that the 5' to 3' nucleotide sequence within the palindrome is the same starting from the 5' (pronounced five prime) ends on both strands of the DNA (the palindrome). EcoRI cuts the DNA molecule in a staggered fashion, leaving "overhang,'' "cohesive'' or "sticky'' ends. In the above example, the overhangs are called 5' overhangs because the unbonded nucleotides have a 5' end. Other restriction enzymes can leave a 3' overhanging end. Another restriction endonuclease (HaeIII), derived from the bacterium Haemophilus aegypticus, snips DNA as shown below. Note that this enzyme cuts both strands at opposite bonds, leaving "blunt" ends.
Restriction enzyme maps for any given DNA segment (linear or circular) can be constructed. Such maps show the location of various restriction endonuclease recognition sites on the DNA fragment. The sizes of the various restriction fragments can be expressed by molecular weight, but more commonly they are given in terms of number of base pairs (bp) or thousands of base pairs (kilobases, kb).
EXAMPLE 12.3 A linear 10,000-bp fragment of DNA has an unknown number of EcoRI restriction enzyme sites. You perform a restriction digestion of this fragment with EcoRI and obtain the following result: three fragments of sizes 5, 3, and 2 kb. These results suggest that there are two EcoRI sites present in this molecule. One possible arrangement of sites (arrows) is shown below. There are two other possible arrangements (not shown).
If this molecule were circular, as are bacterial plasmids commonly used in recombinant DNA technology, only two fragments (3 and 7 kb) would be obtained. The 7 kb fragment is a result of the joining of the 5 and 2 kb segments (see diagram below).
Many other enzymes that are involved in the replication, recombination, repair, modification, transcription, and translation of nucleic acids are utilized in recombinant DNA technology. In particular, DNA synthesis enzymes, known as DNA polymerases, have become very useful for synthesizing copies of DNA molecules in vitro (in a test tube) in a process called the polymerase chain reaction, or PCR (see later section).
Practice problems for these concepts can be found at:
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- Arthropods: Animals with No Spine But Jointed Feet
- Chordata: Animals with a Spine
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Medicare Part D's hopeful lessons for Obamacare
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Congress is back—and the House has an ambitious plan for the year ahead. OK, an ambitious plan to cement its place in history as the Do-Nothingest Congress of all time. The House has scheduled all of 97 days in session before the November elections, with many of them being half days or pro forma ones. And Majority Leader Eric Cantor's memo to his troops outlining the plan for the year ahead made it clear that there is at most a bare-bones agenda, focused like a laser, yet again, on repealing or further sullying and delegitimizing Obamacare.
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Lowell Corporation
DoubleShot® "bolt-thru " ratchet wrenches by Lowell
wrenches. The sockets are made from rugged cast iron and painted florescent orange. To make using the tools more comfortable and secure, each wrench has a rubber coated molded grip. Nine socket combinations are available for heavy hex, standard hex and metric hex fasteners. DS-1 = 1-1/4" x 1-1/16". DS-2 = 1-1/8" x 15/16". DS-3 = 15/16" x 3/4". DS-4 = 3/4" x 7/8". DS-5 = 19 mm x 22 mm. DS-6 = 1-1/8" x 1-1/16". DS-7 = 17 mm x 19 mm. DS-8 = 22 mm x 24 mm. DS-9 = 24 mm x 30 mm... (read more)
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S.I. Tech, Inc.
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Quabbin Wire & Cable Co., Inc.
Thermoplastic Shielded and Unshielded Cables
Quabbin Wire & Cable has stocking locations throughout the USA. Quabbin Wire & Cable's end markets include: Local area network (LAN) systems. Industrial LAN systems. Telecommunications DS-1 and DS-3 systems. Control and instrumentation systems. Process control interconnect. Computer peripherals. Sound and audio instrumentation. Low capacitance data. Fire alarm and security systems. Point of sale equipment. Custom designed signaling applications (read more)
Browse Cable Assemblies Datasheets for Quabbin Wire & Cable Co., Inc.
Parts by Number Top
|Part #
|Product Category
|Panduit Corp
|Cables, Wires - Management
|Newark / element14
|Not Provided
|Emerson Network Power Connectivity Trompeter
|Undefined Category
|Diodes:Silicon Rectifiers:General-Purpose
|Diodes:Silicon Rectifiers:General-Purpose
|National Microchip
|Not Provided
|Not Provided
Conduct Research Top
Cell Master MT8212A T1 Measurements (.pdf)
and coverage maps. What may not always be acknowledged is the ever-expanding wired network supporting the wireless service. Most commonly the "backhaul" links connecting the BTS's to their Mobile Switching Centers (MSC) are based on wired DS1 (or T1) circuits. The quality of the service provided over those T1
Fully Automatic Quality Inspection of Laser Brazed Seams on Decklids of a BMW Car (.pdf)
) DS1-D1024-CL MV-D1024E-CL DS1-D1312-CL MV1-D1312(I/IE)-CL MV1-D1312C-160-CL MV1-D1312(IE/C)-G2 HD1-D1312(IE)-CL/G2 MV1-D1280(I/C)-CL MV1-D1280(I/C)-G2 MV1-D2048x1088(I/C)-CL MV1-D2048x1088(I/C)-G2
How Much Intelligence Does a Camera Need? (.pdf)
Accessories Board Level Cameras OEM-Modules CMOS Sensors Product Applications Product brochure (PDF) Overview MV1-L2048(I/C) DS1-D1024-CL MV-D1024E-CL DS1-D1312-CL MV1-D1312(I/IE)-CL
CMOS Camera Checks Automotive Laser Welds (.pdf)
OEM-Modules CMOS Sensors Product Applications Product brochure (PDF) Overview MV1-L2048(I/C) DS1-D1024-CL MV-D1024E-CL DS1-D1312-CL MV1-D1312(I/IE)-CL MV1-D1312C-160-CL MV1-D1312(IE/C
Fully Automatic Quality Inspection of Laser Welds in a Lap Seam Configuration of a AUDI A3 (.pdf)
Cameras Custom solutions Camera Accessories Board Level Cameras OEM-Modules CMOS Sensors Product Applications Product brochure (PDF) Overview MV1-L2048(I/C) DS1-D1024-CL MV-D1024E-CL
CMOS Cameras of Photonfocus for 3D Laser Measuring Technology (.pdf)
MV1-L2048(I/C) DS1-D1024-CL MV-D1024E-CL DS1-D1312-CL MV1-D1312(I/IE)-CL MV1-D1312C-160-CL MV1-D1312(IE/C)-G2 HD1-D1312(IE)-CL/G2 MV1-D1280(I/C)-CL MV1-D1280(I/C)-G2 MV1-D2048x1088(I/C)-CL
Leica Geosystems Walk-Around Measurement System (.pdf)
) DS1-D1024-CL MV-D1024E-CL DS1-D1312-CL MV1-D1312(I/IE)-CL MV1-D1312C-160-CL MV1-D1312(IE/C)-G2 HD1-D1312(IE)-CL/G2 MV1-D1280(I/C)-CL MV1-D1280(I/C)-G2 MV1-D2048x1088(I/C)-CL MV1-D2048x1088(I/C)-G2
CMOS Cameras Control Laser Welding Process (.pdf)
) Overview MV1-L2048(I/C) DS1-D1024-CL MV-D1024E-CL DS1-D1312-CL MV1-D1312(I/IE)-CL MV1-D1312C-160-CL MV1-D1312(IE/C)-G2 HD1-D1312(IE)-CL/G2 MV1-D1280(I/C)-CL MV1-D1280(I/C)-G2 MV1-D2048x1088(I/C)-CL
More Information Top
Cisco Voice Switch Service Module (VXSM) Command Reference, Release 5.3 - VXSM Commands [Cisco MGX 8880 Media Gateways] - Cisco Systems
clrds0cnt - ds1 .
bay {1 - upper} or bay.line.path. ds1 .
Cisco ONS 15310-CL and Cisco ONS 15310-MA Reference Manual, Release 9.0 - Appendix C, Network Element Defaults [Cisco ONS 15300 Series] - Cisco Systems
DS1 -28/DS3-EC1-3 Card Default Settings .
Configuring DS1 or E1 Lines .
Cisco ONS 15454 Reference Manual, Release 8.5.x - Appendix C, Network Element Defaults [Cisco ONS 15400 Series] - Cisco Systems
•Card—( DS1 /E1-56, ML-series, and FC_MR-4 cards) Transport mode, operating mode, enable/disable retiming, and...