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2 Giants defensive starters injured in Dallas
ARLINGTON, Texas (AP) Giants defensive starters Cullen Jenkins and Jon Beason are out of New York's game against the Dallas Cowboys because of injuries sustained in the first half. Jenkins, the left defensive tackle, sustained a calf strain Sunday. Middle linebacker Beason left the game because of a toe injury. The Giants said after halftime that both players were out for the...
Via AP on Fox
2 days ago | Discuss
Cullen Jenkins makes shocking admission about Chip Kelly's offense
It's no secret that Chip Kelly does everything fast, he even joked that the Eagles set a world record for fastest team photo Monday. Even with that knowledge, Giants defensive tackle Cullen Jenkins admitted Tuesday that the Giants were confused by Chip Kelly's uptempo offense. "We thought we were prepared for it," Giants defensive tackle Cullen Jenkins said on WFAN...
Via Eagledelphia
7 days ago | Discuss
New York Giants Top 10 of 2014: Which defensive lineman came in at No. 10?
Cullen Jenkins came to the New York Giants as an accomplished 10-year veteran coming off a two-year stint with the rival Philadelphia Eagles. Giants fans understandably were skeptic, but the crafty defensive tackle was one of the top stars for the Big Blue defense in 2013. Jenkins ranked among the NFL's top run stoppers at the defensive tackle position, according to Pro Football...
Via cover32 - Giants
May 19, 2014 | Discuss
New York Giants Free Agency: Why Adding Cullen Jenkins is Not a Sign of Things to Come
New York Giants signed former Packers and Eagles DT Cullen Jenkins to a three year-$8 Million deal. Photo courtesy of AP Frank Gray BGB Editor Late last night the New York Giants came to terms with veteran Cullen Jenkins, formerly of the Philadelphia Eagles. This was an extremely important move for three reasons. First, the team needed to bulk up in their interior defense...
Via Big Giants Boom
March 11, 2013 | 1 Comment
Reports: Giants reach agreement with Jenkins
Former Green Bay Packers defensive lineman Cullen Jenkins is a free agent no more. According to NFL.com, Jenkins signed a three-year deal with the New York Giants for 8 million, with 3 million guaranteed. Jenkins, 32, was released by the Philadelphia Eagles on Feb. 25 and had garnered some interest around the NFL. With Green Bay's defensive line needing a boost, the Packers...
Via Fox Sports Wisconsin
March 10, 2013 | Discuss
Cullen Jenkins Close To Reaching An Agreement?
Free agent DL Cullen Jenkins' recent tweet seems to suggest he could be close to signing with a new team. Next chapter beginning??? I think so. — Cullen Jenkins (@CullenJenkins) March 10, 2013 Mike Garafolo reports that there are two teams still in the mix for Jenkins, but the Green Bay Packers are not one of them. Jenny Vrentas reports that the Giants are one of the teams...
Via NFL Trade Rumors
March 10, 2013 | Discuss
Eagles part ways with Cullen Jenkins
Changes in Philadelphia are officially underway. The Eagles announced on Monday that they have released veteran defensive tackle Cullen Jenkins. "I had a chance to speak with Cullen today and let him know of our decision," GM Howie Roseman said, via PhillyMag.com. "It's one of the most difficult parts of the job. He has been a very productive player in this league for...
Via Helmet2Helmet
February 25, 2013 | Discuss
Cullen Jenkins fails at faking injury (VIDEO)
Philadelphia Eagles defensive tackle Cullen Jenkins tried to fake an injury during tonight's game against the Cowboys. It's really sad. I wonder if the NFL will fine Jenkins the way they Emmanuel Sanders of the Steelers. var _CI = _CI || {}; (function() { var script = document.createElement('script'); ref = document.getElementsByTagName('script')[0]; _CI...
Via Pro Football Zone
December 02, 2012 | Discuss
Cullen Jenkins Takes Pay Cut to Stay on the Philadelphia Eagles
Defensive tackle Cullen Jenkins was one of the few consistent bright spots on the Eagles defense in 2011. He was one of the few free agents signed last year that was worth the hype. And often, he seemed to be a lone voice of reality and leadership coming out of the locker room in the dark depths of the first half of the season. Despite the bumps in the road last season, the former...
Via Philly Sports Muse
February 22, 2012 | Discuss
Packers Might Get Cullen Jenkins Back From the Eagles
The Philadelphia Eagles signed defensive end Cullen Jenkins to a five-year $25 million contract in the offseason. On the first day of the new league year, the Eagles will owe Jenkins a $5 million roster bonus, and right now it looks like they're not sure if they'll pay it. If the Eagles decide to not pay Jenkins, he would then become a free agent and the Eagles wouldn't...
Via Pro Football Zone
January 18, 2012 | 2 Comments
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Trinity Renewed: Keller Links Health of Episcopal Church to Community
by Jan Cottingham on Monday, Aug. 25, 2014 12:00 am
The Rev. Christoph Keller III, interim dean and rector of Trinity Cathedral in Little Rock. | (Photo by Michael Pirnique)
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- The Interesting Battle over Alcohol in Arkansas
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13200 Conant St. - Detroit, MI - 11am-2pm
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This is a banana cream pie lovers dream. I had the pleasure of tasting it and it is unbelievable. What do you guys and gals think? Let me know!Read Full Article


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: Gold hack:
Pokemon Bronze [COMPLETED]
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How could someone miraculously come up with the region name I've had since I was 12, and decided on using for my latest hack? THE LUCK!
Kohto was a misconception of Tohjo Falls when I was reminiscing on a lost copy of Gold version, and now someone's using it :/.
What can I do for you to get it back, O black-haired Philip-Fry-person?
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Are you a fan of the Oscar's? Was Oscar night an event in your home? I do not think anyone is more proud of the Oscar Nominees than their moms. Oscar.com caught up with some of this year's Oscar nominees moms to get their reactions. You can see their pride shinning through!
Did your favorites win?!
If you did not get a chance to watch, here is a list of the Oscar Nominees and Winners!
Latest posts by Lauren Elise (Posts)
- Marie Callender's Comfort from Home Project #ComfortsfromHome - October 28, 2014
- Watch the Curious George Halloween Special on PBS KIDS - October 22, 2014
- Spooky Framed Spider Web Halloween Craft - October 19, 2014


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SEATTLE (AP) - Seattle firefighters have rescued a window washer who slipped out of his seat and dangled about 65 feet off the ground outside a building at the Seattle Center civic complex.
KIRO-TV helicopter video showed a fire truck ladder reaching the man shortly after noon Thursday and a firefighter helping him climb down.
Fire Department spokesman Kyle Moore says the man was hanging from a safety rope about 65 feet off the ground at the concert venue McCaw Hall.
Firefighters first went to the roof, about 15 feet above the man, then lowered a harness to secure him until the ladder truck arrived.
(Copyright 2011 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.)


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What goes up no longer has to come down. British scientists have developed an antigravity machine that can float heavy stones, coins and lumps of metal in mid-air. Based around a powerful magnet, the device levitates objects in a similar way to how a maglev train runs above its tracks.
Peter King, a physics professor at Nottingham University, said: "We can take an object and float it in mid-air because the magnetic forces on the object are enough to balance gravity.
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Avocado toast. #egg
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S.O.S. Breakfast Toast //
Basil Pesto & 6 Minute Egg on Toast on camillestyles #Breakfast #Egg #Toast
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Bangkok Dangerous (2008)
59 publicity stills
2 wallpapers
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2 wallpapers
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When carrying out a hit, assassin Joe (Cage) always makes use of the knowledge of the local population. On arriving in Bangkok, Joe meets street kid Kong and he becomes his primary aide. But when Kong is nearly killed, he asks Joe to train him up in the deadly arts and unwittingly becomes a target of a band of killers.Starring: Nicolas Cage, Shahkrit Yamnarm, Panward Hemmanee, James WithSee all
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Deal of the week
With the vogue for funky 4x4 lookalikes, old-school people carriers are about as deirable as socks with sandals, as deals on the Renault Scenic demonstrate. You can get a 1.2 TCE Dynamique TomTom Energy from online dealer Buyanewcaronline.co.uk for £14,942. That's high-end Clio money and a hefty £5,513, or 27 per cent, off the list price. Have you managed to secure a better deal? If so, email firstname.lastname@example.com


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here up for sale is my 14" Mayland fishing boat, complete with mariner 8hp and trailer, recently taken out and just began prepping for new paint colours ready for next season, but due to certain issues with sadness im going to have to let go, engine


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Welcome to samanthab's Member Page
See my Clippings See my Journal See my trade list Send me an email
Hello, My name is Sam and I just recently got into gardening. My major in school is plant, soil & insect sciences. My mother and I just made our first vegetable garden using the square foot method and it is looking good so far!!
I live in: United States
My zone is: z5 MA
My favorite forum 1 is Vegetable Gardening.
My favorite forum 2 is Square Foot Gardening.
First registered on June 08, 2010 .


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BC News
Woman sues ex after failing class
A 22-year-old B.C. woman is suing her ex-boyfriend to pay tuition for a class she says she failed due to distress over the breakup.
Roopam Plawn, a marketing student at Thompson Rivers University in Kamloops, says in her notice of claim that she is seeking $500 for the class, $600 for anxiety, depression, insomnia and loss of working time and performance and $250 for "severe distress."
She also wants Jasmeet Ahluwalia (AHLOO'-wall-ia) to pay court costs.
Plawn says in the court document that she met the international student in September 2013, they broke up in early 2014, got back together again and ended the relationship for good in May.
Plawn also says she lent Ahluwalia $3,500 and she wants the money back, with interest.
None of the allegations have been proven in court, and Ahluwalia has 14 days to respond to the notice after he's been served. (Kamloops This Week)
Comments on this story are pre-moderated and approval times may vary. Before they appear, comments are reviewed by moderators to ensure they meet our submission guidelines. Keep it clean, keep it civil, keep it truthful, stay on topic and be responsible. Comments are open and welcome for three days after the story is published. We reserve the right to close comments before then. Comments that appear on the site are not the opinion of Castanet, but only of the comment writer.
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Oqueue download - forums - world of warcraft - battle.net, Does anyone know where i can download this addon? curse is no longer supported right? the solideice.com link doesn't work..Solidice - home, I am pleased to announce that we have finished the first stages of our development to allow addon authors to upload, claim, and manage new and existing addons on. [addon] "oqueue" find people, create groups, finish that, Oqueue is a simple yet extremely powerful addon that uses blizzards battletag system toist you in finding raid, pvp and fun groups..
★ wow guide - [addon] oqueue premade finder tutorial ft, This is a tutorial for the oqueue premade finder addon. you can download it at the following link. http://www.curse.com/addons/wow/oqueue you can subscribe.Oqueue is "in moderation" on curse. - forums - world of, You can download it directly from the official site. http://solidice.com/oqueue/ oqueue is also on facebook. http://www.facebook.com/groups/oqueue/. Oqueue main thread - forums - world of warcraft, Since the bot problem doesn't seem to get fixed, let's take matters into our own hands. if you are a dedicated pvper like me, who is tired of doing bgs with pugs.
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Audeze has announced that long-time music lifestyle industry veteran Kurt Soto has been named as the company's new marketing director. In this role Soto oversees all aspects of marketing including
Audeze is proud to sponsor the second of four celebrations of music with The Village Recorder and the The Crescent Hotel, Beverly Hills, CA. Discover the next generation of artists in this legendary
Audeze invites you to join them at the Getty Center, Friday August 8th, 6 to 9pm, in the Museum Courtyard for a stunning evening of sight and sound sponsored by Audeze CEO Alex Rosson and Wilco's
We're excited to be a part of Stereo Exchange's inaugural Headphone Event on Thursday, July 31st from 1 to 9pm at 627 Broadway just above Houston Street in NYC. Chat with the experts and win door
Audeze is excited to attend CanMania at the 2014 Capital Audiofest in the Cypress Ballroom of the Sheraton Silver Spring Hotel, 4th floor Meeting Center, 8777 Georgia Avenue, Silver Spring, MD 20910
Over the past few months we have been testing our planar microphones at some prestigious studios with encouraging results.Here is a picture from last week's test.
We're looking forward to exhibiting at the T.H.E. Show Newport at the Hilton Headphonium, May 30th through June 1st. Audeze headphones can be auditioned on a variety of components including the
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Traditional lamb shank recipes usually have wine as the braising liquid, but take one bite of this savory dish and you'll immediately know something is different: beer. Johnson County Community College student chef Rateb Aburas chose beer, particularly an IPA, for braising lamb to create a greater depth of flavor.
Mike Hursey's recipe for Braised Pork Cheeks With Chocolate and Rosemary comes from his passion for using local foods. He buys as many ingredients — from pork to pecans — from small local producers.
David Leonard, a student chef at Johnson County Community College, is not going to say which restaurants inspired his Fennel Roast Chicken With Apricot Champagne Sauce, Grilled Herb Polenta and KC Kale and Cabbage Sauté Vegetable Medley. But he's happy to share his recipes.
Heather Popper was in middle school, dining at an Italian restaurant, the first time she tasted carpaccio. It was love at first bite. Now Popper, a student chef at Johnson County Community College, mostly makes it for her foodie friends or doesn't mention that the meat is raw and covers it heavily with sauce. "This is a simple yet very elegant dish," she said.


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3405 Brooks Ave has a Walk Score of 48 out of 100. This location is a Car-Dependent neighborhood so most errands require a car.
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text: Wind On Capitol Hill March 28, 2005 Issue Softball By Ben McGrath Table of Contents Over coffee in the cafeteria of the Rayburn House Office Building early Thursday morning, several congressional staffers acknowledged the obvious: baseball had come to Washington, and the politics of sport was threatening to supplant the sport of politics. "I bumped into this reporter, and he was all important, like, 'I'm here to cover the hearings,' " one man said. "Well, we have hearings here every day. I mean, I like baseball, but our priorities are out of order." "It's hero-worship, that's all it is," another man replied, shaking his head. Upstairs, inside the chambers of the Committee on Government Reform, the steroid hearings soon began—and demonstrated that hero-worship is thriving, in the halls of Congress, at least. Jim Bunning, the junior senator from Kentucky, and a Hall of Fame pitcher, was the first to testify, and encomiums were quick to follow. Candice Miller (R., Mich.) wanted to let Bunning know that she was grateful that he'd pitched for her home-town Detroit Tigers. She'd even heard he threw a no-hitter. John McHugh (R., N.Y.), for his part, said that he'd visited Cooperstown in 1996 to watch Bunning's Hall of Fame induction—a memorable experience. Nearly eight hours later, after various passing tributes to Nellie Fox, Mickey Mantle, and Henry Aaron, the urge to be a part of the national pastime had not subsided. Linda Sanchez (D., Calif.) leaned forward and spoke into her microphone: "Let me just start by saying that I'm a huge baseball fan." Jose Serrano (D., N.Y.) looked out at the panel of assembled ballplayers—seated, left to right: Mr. Jose Canseco, Mr. Sammy Sosa, Mr. Mark McGwire, Mr. Rafael Palmeiro, Mr. Curt Schilling—and asked them not to think of his colleagues as legislators. "Think of us as no different than the people you see in the stands," he said. Meanwhile, some of the athletes seemed to be testing their political chops. Mark McGwire's teary insistence that we focus on positives, not negatives, his repeated assertions that he was there to talk about the future, not the past—it all might have played better on the stump. And Curt Schilling, the most actively political of the bunch (he campaigned for President Bush last fall, and seems even to have adopted Bush's walk), saw fit to mention that his father, his brother-in-law, and his cousin had all worn the uniform—the military uniform. During an intermission, he lingered to shake hands with the parents of a teen-ager who'd committed suicide after quitting steroids. "Maybe we can make a difference," Schilling said, and winked. There was some genuine antagonism in the room, as when Elijah Cummings (D., Md.) lectured Elliot Pellman, a Major League Baseball physician: "In the words of my mother, you've got motion, commotion, emotion, and no results." But for every Cummings there was an Ileana Ros-Lehtinen (R., Fla.), who, as the proceedings dragged on into evening, or what the committee chairman, Tom Davis (R., Va.), called "extra innings," actually saw fit to re-read Rafael Palmeiro's irrelevant testimony: "My parents and I came to the United States after fleeing the Communist tyranny that still reigns over my homeland of Cuba. We came seeking freedom." Her priorities in order, she hastened to add that Palmeiro was a courageous opponent of Fidel Castro. Gil Gutknecht (R., Minn.) said at one point, "This has been one of the most fascinating hearings I have ever participated in, and I've been in Congress now ten years." Thus informed, a congressional novice, sitting in the back, vowed never to return. In the course of eleven hours, no new information emerged about who has used steroids. Actually, strike that: Paul Kanjorski (D., Penn.) returned from a break and reported that a member of Congress had just confessed to using steroids as a teen-ager, back in 1967. ("They were animal-grade," he said.) Like the players, though, Kanjorski wasn't naming any names. The best remark of the day came, oddly, from Mark McGwire. Responding to a question about what he could do to alleviate the steroid problem, he said, "Whatever anybody can do to improve it so there's no more meetings like this, I'm all for it." The motion is hereby seconded. Ben McGrath began working at The New Yorker in 1999, and has been a staff writer since 2003. Bio All work & You Might Like Jigsaw Puzzle Strongbox Submissions Goings On App About Us Caption Contest The New Yorker Festival ✕ The New Yorker Strongbox Strongbox is a new way for you to share information, messages, and files with our writers and editors and is designed to provide you with a greater degree of anonymity and security than afforded by conventional e-mail. To help protect your anonymity, Strongbox is only accessible using the Tor network (https://www.torproject.org). When using Strongbox, The New Yorker will not record your I.P. address or information about your browser, computer, or operating system, nor will we embed third-party content or deliver cookies to your browser. You can read our full privacy promise here. The New Yorker Strongbox is powered by SecureDrop. To get to Strongbox and begin using it to contact writers and editors at The New Yorker, just follow these two steps: 1Download and install software to access the Tor Project: https://www.torproject.org. 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A team of personal 'Stylizers' will primp and preen a group of friends and transform them into red carpet goddesses during a retail therapy weekend at Stayingcool (0161 832 4060), a stylish hotel-apartment development in Manchester. A champagne reception kicks off the weekend, then you'll have a style party before being guided round the city's shops the next day. The team will pre-book changing rooms, then organise bars, clubs and restaurants for Saturday night. Cost £370pp including champagne reception, stylist, accommodation and breakfast.
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These handsome boys were born August 10th and are up to date on shots and worming. Gunner, white chin and Grant, white toes should be medium to large size when fully grown. Their mom is a purebred shepherd their dad a mix of Collie and Border collie, both have very sweet ...
Oct 18 -
Detroit :
Memphis - Dogs & puppies
Border Collie - Leo - Large - Young - Male - Dog
You can fill out an adoption application online on our official website.Leo is an active,fun loving handsome fellow. He is looking for a loving active family. He does good with other dogs but needs a small animal free home. ...
Oct 18 -
Roanoke :
Roanoke - Dogs & puppies
2- chocolate Merle females and two Tri-Blue Merle males! Ready to go to new homes November 15th. Very playful. Raised in our home and are being introduced cattle. Will not ship puppies and good homes are a must!!!
**** only one male is looking for new home. The females and ...
Oct 15 -
Erie :
Linesville - Dogs & puppies
Collie - Baby Diva - Medium - Baby - Female - Dog
Scrolling List: http://members.petfinder.com/~MI730/scroll_pets.html +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ My name is Diva and that's what I want to be when I grow up, but in the ...
Oct 13 -
Detroit :
Utica - Dogs & puppies
Collie - Leland - Large - Young - Female - Dog
Leland, #1804, is a beautiful 7 month old Collie/Shepherd mix pup. Leland is a very sweet dog who does well with other dogs. We love her crazy ears! Leland is a sweet dog seeking a loving home. The Marshall County Animal ...
Oct 11 -
Pittsburgh :
Moundsville - Dogs & puppies
My husband and I have two beautiful full blooded Border Collie's that had puppies. There are 11 all together, 6 girls and 5 boys. They are the classic black and white.They were born on U.S Constitution day (September 17) and will be ready for homes by November 4th. We ...
Oct 10 -
Cincinnati :
Waynesville - Dogs & puppies
3 male Border collie puppies still available born 8/28/2014! The mother is b&w, and their father is r&w, both on premises. Pups will be dewormed & have first shots, and be ABCA registered with papers. Pups will be around our children, a kitten, & chickens! Pups will have a ...
Oct 10 -
Erie :
Titusville - Dogs & puppies
Border Collie - Haven~nice Companion! Very Sweet - Medium
Nice all around pleasant dog!! Great disposition. Haven was on her last day at a rural Ohio dog pound..Time was up so Dogworks agreed to save this girl and take her into rescue. Medium size Haven we believe is Border ...
Oct 9 -
Toledo :
Toledo - Dogs & puppies
Red and White Female Border Collies - Registered
- All Shots and Wormed
- Birthdate - August 15th 2014
- Registered American Border Collie Association
- Outstanding Pedigree
Oct 7 -
Louisville :
Scottsburg - Dogs & puppies
Collie - Bear - Large - Senior - Male - Dog
Bear was surrendered to Alchemy when his owner lost his home. This poor old guy is definitely in need of a home just to live out his last few years. Bear does have a few medical concerns that are common with old age. Personality ...
Oct 4 -
Northeast Ohio :
Salem - Dogs & puppies
Terrier - Duke - In Training - Medium - Young - Male - Dog
Hi Folks! My name is Duke, a two year old Terrier/Border Collie mix. If you are seeking a true friend and companion, Im the guy for you. I am a beautiful black and white with almost a bluish/gray ticking; what I am ...
Oct 3 -
Fort Wayne :
Coldwater - Dogs & puppies
Rescued Mixed Breed Boxer Border Collie Pups ....Boys and Girls!! 12 Weeks old! Very Sweet and Playful - First Shots and Worming Done
Oct 2 -
Northeast Ohio :
East Sparta - Dogs & puppies
Collie - Bo And Rascal - Medium - Young - Male - Dog
Bonded Pair. Must be adopted together. Bo (50 lbs) and Rascal (46 lbs) are two very silly boys who came into the shelter together, as their owner could no longer care for them. They are litter mates and approx. 2 years ...
Oct 1 -
Indianapolis :
Anderson - Dogs & puppies
Beautiful active border collie pups! They are weaned and ready for their new homes. 2 Females, 3 males left. Mother is strong on agility, father is strong on herding. Best of both worlds. These pups have loads of potential. Both parents were ABCA registerable but I did not ...
Sep 29 -
Blacksburg :
Rocky Mount - Dogs & puppies
Border Collie - Rosella - Medium - Adult - Female - Dog
EDOB 08/20/12 Wt. # 39.7 Rosella is a friendly, intelligent and athletic girl! She would do well with someone that will take her on daily walks and help keep her mind challenged. She loves attention and enjoys being ...
Sep 27 -
Cincinnati :
Batavia - Dogs & puppies
Baby puppies mother got hit on the road and these babies need new homes!! Email me at [email removed] or call 330 222 7037 one pup has a blue eye and brown eye
Sep 26 -
Northeast Ohio :
Salem - Dogs & puppies
Shepherd - Spot - Medium - Adult - Male - Dog
He is goofy, playful, fun loving.its Spot! Spot is a handsome Shepherd/lab mix with a smile that will light up the room. He is very confused why he is in the noisy shelter and would love to be in a home with a family of his own. ...
Sep 26 -
Indianapolis :
Anderson - Dogs & puppies
Border Collie - Wally - Medium - Young - Male - Dog
Woof!!! I'm Wally and I am a happy go lucky pup. I like everyone and I sure hope everyone likes me. I know I have a lot to learn,. I hear that from the older dogs here at ISBCR rescue but I am ready to start learning all ...
Sep 25 -
Northeast Ohio :
Minerva - Dogs & puppies
Border Collie - Maria - Medium - Adult - Female - Dog
Darling Maria is full of life and love. She loves people and loves to play. She is just energentic enough but not too much to handle. Maria loves to go for walks just as much as she loves to lounge on the couch.All ...
Sep 23 -
Northeast Ohio :
Salem - Dogs & puppies
We have 6 beautiful border collie puppies looking for forever homes..they have been given 1st shots and dewormed..raised with children and other animals..they have been bottle fed since the mother had passed away during delivery. Spoiled and loveable. They are all black and ...
Sep 22 -
Indianapolis :
Anderson - Dogs & puppies
Our babies come up to date on their shots and given an inter-nasal bordetella vaccine.
The are wormed every two weeks until they leave for their forever homes.
They come up to date on heart worm and flea prevention. They are vet checked and micro-chipped before they go to ...
Sep 22 -
Northeast Ohio :
Columbiana - Dogs & puppies
Border collie puppies born 8/28/2014, lots of males! They seem to all be B&W. The mother is b&w, and their father is r&w, both on premises. Pups will be dewormed & have first shots, and be ABCA registered with papers. Pups will be around our children, a kitten, & chickens! ...
Sep 21 -
Erie :
Titusville - Dogs & puppies
Indigo is a beautiful blue/white girl, She is very loving an loyal. She is a full working line carries some sagebrush dogs, she has several imports as well. Indy has a lot of drive for work an play, she can come in an be a nice house dog. She carries red tri an gold, she ...
Sep 16 -
Huntington-Ashland :
Wheelersburg - Dogs & puppies
For Sale, First generation Aussiedoodle puppies. (Purebred Australian Shepard X AKC cream standard poodle.) These pups are very well socialized with great personalities. Hypoallergenic, low to no shed, smart, and will make a great addition to you family. Chocolates, Creams, ...
Sep 12 -
Cincinnati :
Maysville - Dogs & puppies
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Snack Attack. The Corner Store has all of your snacking needs. Chips to nuts
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Harry's Featured in the Food and Drink Section. What a write up thanks Wade Tatangelo! […]
Harry's Continental Kitchens
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© 2014 Harry's Continental Kitchens
DESIGN: Clark Creative


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Is there any workplace topic more incendiary than compensation? Doesn't really matter if you are on the Owner's side of the topic or a regular employee. Equally, this can bring up buried emotions, for the workplace.
There is no hard and fast rule about doling out raises and bonuses that I know of, but I think every company should have a policy that guides them. Without any compensation policy, how does a manager determine when a raise might be appropriate? How much of a raise should be given? How often should an increase occur?
How about: managers should discuss some type of monetary reward, raise, or bonus when it is EARNED. Any day might be an appropriate day! How about: does it matter how much of a raise is EARNED? Could be fifty cents, a dollar, or a flat out bonus. How about: any time!
Okay, that sounds great to me, in theory. However, what happens when you have a three member Leasing Team who are friendly with one another and eat lunch together sometimes, and maybe even go out together after work? They talk. One of them mentions he/she received a raise and is celebrating, not thinking anything about it, or assuming they had all received an increase. "Really? Why?" one of them asks? The third person seethes inside silently as he/she ponders why he/she DIDN'T receive a raise, too.
The concept of "fairness" can tear a team apart if the appearance of unequal pay is at issue, especially when a sense of friendly competition is encouraged. Or, worse, if the team is expected to work together on all leases. For example, Leasing Consultant 1 answers the phone and books the appointment, but the only time the Prospect can visit the property is when Leasing Consultant 1 is off. Leasing Consultant 2 shows the Prospect around the property and makes every attempt to close the deal. Unfortunately, the Prospect is just not ready to commit and wants to continue shopping. It is three weeks later when the Prospect returns and Leasing Consultant 1 is not available and takes the Be-Back appointment and the Prospect tours a second apartment and fills out and application. Leasing Consultant 1 then processes the application, follows up on scheduling the move in date. Enter Leasing Consultant 3 who actually is the only one available on the now new Resident's move in day. Leasing Consultant 3 collects the money, does the initial move in inspection with the Resident, submits a couple of work orders and is the one who delivers a move in gift to the Resident the next day with a note of thanks.
Leasing is often a team event. Awarding a bonus to only one these professionals seem wrong to me. Most offices may offer a split commission and that would be the end of the matter. The prudent thing to do is to make sure there is a Policy in place that covers this topic, as well as, when ALL employees might expect a raise or Bonus. Many companies offer not only commissions, but also Bonuses. If the company offers raises at an employee's anniversary date, then this should also be done for ALL employees. The worst thing a company can do is to offer extra compensation to its star players when so often it is a team event. Put your policy in writing and have your teams sign it for their personnel file so at the very least everyone understands and can anticipate when such additional compensation can be earned, whether that is annual raises, monthly bonuses, or commissions.


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Monday Morning Dump
May 21st, 2012
It's Monday again, so steady yourself for our weekly collection of pictorial pleasure perfection. Another batch of the juiciest nuggets of internet gold mushed together and squeezed into a vaguely cohesive whole for your admiration. Enjoy.
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Are you K H Winterhalter?Claim your profile
Publications (187)832.98 Total impact
Article: Purification, crystallisation and X-ray diffraction study of fully functional laccases from two ligninolytic fungi.[Show abstract] [Hide abstract]
ABSTRACT: Laccase isozymes from the white-rot basidiomycete fungi Trametes versicolor and Pycnoporus cinnabarinus were purified to apparent iso-electric homogeneity and crystallised. T. versicolor laccase crystallises in two crystal forms, both with the orthorhombic space group P2(1)2(1)2(1), which diffract to 1.9 and 2.95 A resolution, respectively. The crystals of P. cinnabarinus laccase belong to the monoclinic space group C2 and diffract to at least 2.2 A resolution. All the laccase crystals are suitable for X-ray structure determination and contain a full complement of copper ions.Biochimica et Biophysica Acta 02/2002; 1594(1):109-14. · 4.66 Impact Factor
Article: Identification of a novel neuroligin in humans which binds to PSD-95 and has a widespread expression.[Show abstract] [Hide abstract]
ABSTRACT: Neuroligins, first discovered in rat brain, form a family of three synaptically enriched membrane proteins. Using reverse transcription-PCR of human brain polyadenylated RNA and extensive database searches, we identified the human homologues of the three rat neuroligins and a cDNA encoding a fourth member, which we named neuroligin 4. Neuroligin 4 has 63-73% amino acid identity with the other members of the human neuroligin family, and the same predicted domain structure. DNA database analyses, furthermore, indicated that a possible fifth neuroligin gene may be present in the human genome. Northern-blot analysis revealed expression of neuroligin 4 in heart, liver, skeletal muscle and pancreas, but barely at all in brain. Overexpression of neuroligin 4 cDNA in COS-7 cells led to the production of a 110 kDa protein. Immunofluorescence analysis demonstrated that the protein was integrated into the plasma membrane. Overexpression of cDNAs encoding neuroligin 4 and the PDZ-domain protein, PSD-95, in COS-7 cells resulted in the formation of detergent-resistant complexes. Neuroligin 4 did not bind to ZO-1, another PDZ-domain protein. Together, our data show that the human neuroligin family is composed of at least one additional member, and suggest that neuroligin 4 may also be produced outside the central nervous system.Biochemical Journal 07/2001; 356(Pt 2):581-8. · 4.65 Impact Factor
Article: Structure elucidation of beta-mannanase: from the electron-density map to the DNA sequence.[Show abstract] [Hide abstract]
ABSTRACT: The crystal structure of affinity-purified Thermomonospora fusca beta-mannanase has been solved despite the lack of the major part of the amino-acid sequence. A high-quality electron-density map allowed the identification of a stretch of eight amino acids close to the C-terminus which was used to design a degenerate downstream PCR primer. Together with a specific primer previously derived from the N-terminus, 95.7% of the mannanase gene sequence was obtained from genomic T. fusca DNA by PCR. The structure-derived sequence was then compared with the DNA-derived sequence and corrected when necessary. Applying the presented protocol, there was no need to manually build a model at an early stage of structure determination, an erroneous and tedious process, especially in the absence of the amino-acid sequence. Using the DNA sequence information and the current version of ARP/wARP, 281 residues, or 93% of the polypeptide chain (including side chains), were built and refined to an R factor of 16.5% without any manual intervention.Acta Crystallographica Section D Biological Crystallography 02/2001; 57(Pt 1):37-43. · 14.10 Impact Factor
Article: Localization of organic anion transporting polypeptide 4 (Oatp4) in rat liver and comparison of its substrate specificity with Oatp1, Oatp2 and Oatp3[Show abstract] [Hide abstract]
ABSTRACT: Organic anion transporting polypeptides (rodents: Oatps; human: OATPs) are involved in the absorption and elimination of a wide variety of structurally unrelated amphipathic organic compounds. Several members of this protein family mediate the uptake of substrates across the basolateral membrane of hepatocytes as the first step in hepatic elimination. In contrast to the well-characterized Oatp1 and Oatp2, the localization and substrate specificity of the recently cloned Oatp4 have not been investigated in detail. Therefore, we raised an antibody against the C-terminal end of Oatp4 and localized this 85-kDa protein to the basolateral membrane of rat hepatocytes. Similar to Oatp1 and Oatp2, Oatp4 is a multispecific transporter with high affinities for bromosulfophthalein, dehydroepiandrosterone sulfate, leukotriene C4, and anionic peptides. In addition, we compared the substrate specificity of Oatp4 to that of Oatp3, which so far has mainly been shown to mediate intestinal bile acid transport. Oatp3 had a similar broad substrate specificity, but in general much lower affinities than Oatp4. Thus, while Oatp4 seems to work in concert with Oatp1 and Oatp2 in the basolateral membrane of rat hepatocytes, Oatp3 is a multispecific transport system in the small intestine.Pflügers Archiv - European Journal of Physiology 01/2001; 443(2):188-195. · 4.87 Impact Factor
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ABSTRACT: The crystal structure of affinity-purified Thermomonospora fuscaβ-mannanase has been solved despite the lack of the major part of the amino-acid sequence. A high-quality electron-density map allowed the identification of a stretch of eight amino acids close to the C-terminus which was used to design a degenerate downstream PCR primer. Together with a specific primer previously derived from the N-terminus, 95.7% of the mannanase gene sequence was obtained from genomic T. fusca DNA by PCR. The structure-derived sequence was then compared with the DNA-derived sequence and corrected when necessary. Applying the presented protocol, there was no need to manually build a model at an early stage of structure determination, an erroneous and tedious process, especially in the absence of the amino-acid sequence. Using the DNA sequence information and the current version of ARP/wARP, 281 residues, or 93% of the polypeptide chain (including side chains), were built and refined to an R factor of 16.5% without any manual intervention.Acta Crystallographica Section D Biological Crystallography 01/2001; 57(1):37-43. · 12.67 Impact Factor
Article: Identification of a novel neuroligin in humans which binds to PSD95 and has a widespread expressionBiochemical Journal - BIOCHEM J. 01/2001; 356(2).
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ABSTRACT: In this paper, we identify the chondroitin sulfate proteoglycan versican V2 as a major inhibitor of axonal growth in the extracellular matrix of the mature central nervous system. In immunohistochemical and in situ hybridization experiments we show that this tissue-specific splice variant of versican is predominantly present in myelinated fiber tracts of the brain and in the optic nerve, most likely being expressed by oligodendrocytes. We demonstrate that isolated versican V2 strongly inhibits neurite outgrowth of central and peripheral neurons in stripe-choice assays using laminin-1 as permissive substrate. The inhibitory character of versican V2 is maintained after removal of chondroitin sulfate and N- and O-linked oligosaccharide side chains, but it is abolished after core protein digestion with proteinase-K. Our data support the notion, that intact versican V2 prevents excessive axonal growth during late phases of development and hereby participates in the structural stabilization of the mature central nervous system.Journal of Cell Science 04/2000; 113 ( Pt 5):807-16. · 5.88 Impact Factor
Article: Occludin proteolysis and increased permeability in endothelial cells through tyrosine phosphatase inhibition.[Show abstract] [Hide abstract]
ABSTRACT: Regulation of epithelial and endothelial permeability is essential for proper function of compartmentalized organisms, and tyrosine phosphorylation plays an important role in this process. We analyzed the impact of protein tyrosine phosphatase (PTP) inhibition on the structure of endothelial junctional proteins. In human umbilical vein endothelial cells (HUVECs) the PTP inhibitors phenylarsine oxide (PAO) and pervanadate induced proteolysis of the tight junction protein occludin. Occludin proteolysis was inhibited by the metalloproteinase inhibitor 1,10-phenanthroline (PHEN), but not by inhibitors against other types of proteases. The junctional proteins ZO-1, cadherin and beta-catenin were not cleaved. Under conditions of occludin proteolysis, PAO treatment elevated permeability for FITC-dextran. Simultaneous incubation of HUVECs with PAO and PHEN inhibited the rise in permeability by more than 60%. PAO treatment lead to progressive disappearance of occludin from the cell periphery. In contrast, ZO-1, cadherin and beta-catenin retained their positions at the sites of intercellular contact. Simultaneous administration of PAO and PHEN greatly prevented the redistribution of occludin. These results demonstrate a selective cleavage of occludin by a metalloproteinase and suggest that this process can contribute to the control of paracellular permeability in endothelial cells.Journal of Cell Science 01/2000; 112 ( Pt 23):4347-56. · 5.88 Impact Factor
Article: Evidence from spin-trapping for a transient radical on tryptophan residue 171 of lignin peroxidase.[Show abstract] [Hide abstract]
ABSTRACT: The heme enzyme lignin peroxidase contains a unique Cbeta-hydroxylated tryptophan residue (Trp171) on the surface of the enzyme. Mutagenetic substitution of Trp171 abolishes completely the veratryl alcohol oxidation activity of the enzyme. This led us to surmise that Trp171 may be involved in electron transfer from natural substrates to the heme cofactor. Here we present evidence for the formation of a transient radical on Trp171 using spin-trapping in combination with peptide mapping. The spin-trap methyl nitroso propane forms a covalent adduct with Trp171 in the presence of hydrogen peroxide which can be detected by its characteristic visible absorbance spectrum. A very similar chromophore can be obtained in a small molecular model system from N-acetyl tryptophanamide, the spin-trap, and a single-electron abstracting system. The precise site the spin-trap is attached to could be identified in a crystal structure of spin-trap/hydrogen peroxide-treated enzyme as the C6 atom of the indole ring of Trp171. These results indicate that Trp171 is redox-active and that it forms an indole radical by transfer of an electron to the heme of compound I and/or II. Apart from cytochrome c peroxidase and DNA photolyase, lignin peroxidase appears to be the third enzyme only which utilizes a tryptophan residue as an integral part of its redox catalysis.Archives of Biochemistry and Biophysics 11/1999; 370(1):86-92. · 3.37 Impact Factor
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ABSTRACT: Sterol carrier protein 2 (SCP2) is a basic intracellular protein which facilitates the in vitro intermembrane transfer of cholesterol, phospholipids and glycolipids. SCP2 was expressed in Escherichia coli, purified to apparent electrophoretic homogeneity and crystallized. Single crystals were obtained by hanging-drop vapour diffusion using ammonium sulfate as precipitant. These crystals belong to space group P4(1)2(1)2 or its enantiomorph, with unit-cell parameters a = b = 57.5, c = 86.5 A, and have one molecule in the crystallographic asymmetric unit. Intensity data to 1.8 A resolution were collected from native SCP2 crystals using synchrotron radiation, were processed and scaled with an R(linear) = 4.9%.Acta Crystallographica Section D Biological Crystallography 09/1999; 55(Pt 8):1478-80. · 14.10 Impact Factor
Article: Purification, crystallization and preliminary crystallographic studies of a two fibronectin type-III domain segment from chicken tenascin encompassing the heparin- and contactin-binding regions.[Show abstract] [Hide abstract]
ABSTRACT: A fragment of chicken tenascin consisting of fibronectin type-III domains 5 and 6 has been expressed in Escherichia coli. After modifying a previously reported purification protocol, an electrophoretically homogeneous recombinant protein was obtained from which various crystal forms could be grown under identical conditions. Only one form was suitable for structure determination. These crystals belong to space group P21, with unit-cell parameters a = 45.2, b = 57.9, c = 72.2 A, beta = 91.4 degrees, and diffract to at least 2.6 A resolution using synchrotron radiation. From density measurements of the crystals, it was found that there are two molecules in the asymmetric unit. Diffraction data of native, two platinum-derivative and one palladium-derivative crystals were collected.Acta Crystallographica Section D Biological Crystallography 06/1999; 55(Pt 5):1069-73. · 14.10 Impact Factor
Article: The crystal structure of lignin peroxidase at 1.70 A resolution reveals a hydroxy group on the cbeta of tryptophan 171: a novel radical site formed during the redox cycle.[Show abstract] [Hide abstract]
ABSTRACT: The crystal structure of lignin peroxidase (LiP) from the white rot fungus Phanerochaete chrysosporium was refined to an R-factor of 16.2 % utilizing synchrotron data in the resolution range from 10 to 1.7 A. The final model comprises all 343 amino acid residues, 370 water molecules, the heme, four carbohydrates, and two calcium ions. Lignin peroxidase shows the typical peroxidase fold and the heme has a close environment as found in other peroxidases. During refinement of the LiP model an unprecedented modification of an amino acid was recognized. The surface residue tryptophan 171 in LiP is stereospecifically hydroxylated at the Cbeta atom due to an autocatalytic process. We propose that during the catalytic cycle of LiP a transient radical at Trp171 occurs that is different from those previously assumed for this type of peroxidase. Recently, the existence of a second substrate-binding site centered at Trp171 has been reported, by us which is different from the "classical heme edge" site found in other peroxidases. Here, we report evidence for a radical formation at Trp171 using spin trapping, which supports the concept of Trp171 being a redox active amino acid and being involved in the oxidation of veratryl alcohol. On the basis of our current model, an electron pathway from Trp171 to the heme is envisaged, relevant for the oxidation of veratryl alcohol and possibly lignin. Beside the opening leading to the heme edge, which can accommodate small aromatic substrate molecules, a smaller channel giving access to the distal heme pocket was identified that is large enough for molecules such as hydrogen peroxide. Furthermore, it was found that in LiP the bond between the heme iron and the Nepsilon2 atom of the proximal histidine residue is significantly longer than in cytochrome c peroxidase (CcP). The weaker Fe-N bond in LiP renders the heme more electron deficient and destabilizes high oxidation states, which could explain the higher redox potential of LiP as compared to CcP.Journal of Molecular Biology 03/1999; 286(3):809-27. · 3.91 Impact Factor
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ABSTRACT: Descriptions for tenascin-C distribution are largely restricted to epithelial tumours. The present study utilized newly developed and characterized monoclonal (hT191) and polyclonal antibodies to investigate the distribution pattern of tenascin-C in a panel of mesenchymal tumours, which was contrasted with normal tissue. The specific antibodies recognized the distinctive star-like hexabrachion protein isolated from transformed cell-culture medium and serum from normal individuals. In normal tissues, a strong tenascin-C expression in the extracellular matrix was largely restricted to basement-membrane regions of epithelium and tonsilar sinusoids, pericellularly within smooth-muscle bundles, associated with perimysial, -chondrial, -neurial and -tendon surfaces, and diffusely within vascular adventitia. It was found in the corresponding tumours of the neural sheath (schwannoma) and smooth muscle (leiomyosarcoma), and was abundantly present around certain blood vessels of mesenchymal tumours. Although not detected in normal muscle, or in adipose or fibrous connective tissue, neo-expression of tenascin-C was shown in more than half of the rhabdomyosarcomas, fibromas and liposarcomas, with an increased positive percentage in variably malignant myxoid liposarcomas compared with lipoma-like sarcomas. Tenascin-C was typically found in the extracellular matrix of soft-tissue tumours, but was notably absent from the epithelial-cell components of mixed epithelial/mesenchymal tumours. Its apparently enhanced expression in soft-tissue tumours differs from that of most other large extracellular-matrix proteins, suggesting possible functional involvement of the cell-adhesion molecule, tenascin-C, in the neoplastic phenotype. Int. J. Cancer 72:217–224, 1997. © 1997 Wiley-Liss, Inc.International Journal of Cancer 12/1998; 72(2):217 - 224. · 6.20 Impact Factor
Article: High-resolution native and complex structures of thermostable beta-mannanase from Thermomonospora fusca - substrate specificity in glycosyl hydrolase family 5.[Show abstract] [Hide abstract]
ABSTRACT: . beta-Mannanases hydrolyse the O-glycosidic bonds in mannan, a hemicellulose constituent of plants. These enzymes have potential use in pulp and paper production and are of significant biotechnological interest. Thermostable beta-mannanases would be particularly useful due to their high temperature optimum and broad pH tolerance. The thermophilic actinomycete Thermomonospora fusca secretes at least one beta-mannanase (molecular mass 38 kDa) with a temperature optimum of 80 degreesC. No three-dimensional structure of a mannan-degrading enzyme has been reported until now. . The crystal structure of the thermostable beta-mannanase from T. fusca has been determined by the multiple isomorphous replacement method and refined to 1.5 A resolution. In addition to the native enzyme, the structures of the mannotriose- and mannohexaose-bound forms of the enzyme have been determined to resolutions of 1.9 A and 1.6 A, respectively. . Analysis of the -1 subsite of T. fusca mannanase reveals neither a favourable interaction towards the axial HO-C(2) nor a discrimination against the equatorial hydroxyl group of gluco-configurated substrates. We propose that selectivity arises from two possible mechanisms: a hydrophobic interaction of the substrate with Val263, conserved in family 5 bacterial mannanases, which discriminates between the different conformations of the hydroxymethyl group in native mannan and cellulose; and/or a specific interaction between Asp259 and the axial hydroxyl group at the C(2) of the substrate in the -2 subsite. Compared with the catalytic clefts of family 5 cellulases, the groove of T. fusca mannanase has a strongly reduced number of aromatic residues providing platforms for stacking with the substrate. This deletion of every second platform is in good agreement with the orientation of the axial hydroxyl groups in mannan.Structure 12/1998; 6(11):1433-44. · 5.99 Impact Factor
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ABSTRACT: cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G (PKG)-specific inhibitor KT5823, indicating that cGMP-mediated activation of PKG leads to inhibition of the pump. A similar extent of inhibition is obtained with nitric oxide. cGMP-induced inhibition acts mainly on alpha1 isoforms but hardly affects alpha2/alpha3 isoforms. These data suggest that inhibition of Na,K-ATPase activity by cGMP occurs in an isoform-selective manner in brain endothelial cells.FEBS Letters 11/1998; 436(3):466-70. · 3.58 Impact Factor
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ABSTRACT: In the high-resolution crystal structures of two lignin peroxidase isozymes from the white rot fungus Phanerochaete chrysosporium a significant electron density at single bond distance from the C beta of Trp171 was observed and interpreted as a hydroxy group. To further clarify the nature of this feature, we carried out tryptic digestion of the enzyme and isolated the Trp171 containing peptide. Under ambient conditions, this peptide shows an absorbance spectrum typical of tryptophan. At elevated temperature, however, the formation of an unusual absorbance spectrum with lambda max = 333 nm can be followed that is identical to that of N-acetyl-alpha, beta-didehydrotryptophanamide, resulting upon water elimination from beta-hydroxy tryptophan. The Trp171 containing tryptic peptide isolated from the recombinant and refolded lignin peroxidase produced from Escherichia coli does not contain the characteristic 333 nm absorbance band at any temperature. However, treatment with 3 equiv of H2O2 leads to complete hydroxylation of Trp171. Reducing substrates compete with this process, e.g., in the presence of 0.5 mM veratryl alcohol, about 7 equiv of H2O2 is necessary for complete modification. We conclude that the hydroxylation at the C beta of Trp171 is an autocatalytic reaction which occurs readily under conditions of natural turnover, e.g., in the ligninolytic cultures of P. chrysosporium, which are known to contain an oxidase-based H2O2-generating system. No dependence on dioxygen was found for this oxidative process. Chemical modification of fungal lignin peroxidase with the tryptophan-specific agent N-bromo succinimide leads to a drastically reduced activity with respect to the substrate veratryl alcohol. This suggests that Trp171 is involved in catalysis and that electron transfer from this surface residue to the oxidized heme cofactor is possible under steady-state conditions.Biochemistry 07/1998; 37(25):8832-8. · 3.38 Impact Factor
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ABSTRACT: We have isolated and characterized the proteoglycan isoforms of versican from bovine brain extracts. Our approach included (i) cDNA cloning and sequencing of the entire open reading frame encoding the bovine versican splice variants; (ii) preparation of antibodies against bovine versican using recombinant core protein fragments and synthetic peptides; (iii) isolation of versican isoforms by ammonium sulfate precipitation followed by anion exchange and hyaluronan affinity chromatography; and (iv) characterization by SDS-polyacrylamide gel electrophoresis and Coomassie Blue staining or immunoblotting. Our results demonstrate that versican V2 is, together with brevican, a major component of the mature brain extracellular matrix. Versicans V0 and V1 are only present in relatively small amounts. Versican V2 migrates after chondroitinase ABC digestion with an apparent molecular mass of about 400 kDa, whereas it barely enters a 4-15% polyacrylamide gel without the enzyme treatment. The 400-kDa product is recognized by antibodies against the glycosaminoglycan-alpha domain and against synthetic NH2- and COOH-terminal peptides. Our preparations contain no major proteolytic products of versican, e.g. hyaluronectin or glial hyaluronate-binding protein. Having biochemical quantities of versican V2 available will allow us to test its putative modulatory role in neuronal cell adhesion and axonal growth.Journal of Biological Chemistry 07/1998; 273(25):15758-64. · 4.65 Impact Factor
Article: DNA methylation accounts for the inhibition of collagen VI expression in transformed fibroblasts.[Show abstract] [Hide abstract]
ABSTRACT: The expression of collagen VI, an adhesive glycoprotein of the extracellular matrix, is completely inhibited in virally transformed fibroblasts and in many cell lines derived from spontaneous mesenchymal tumors. Here we present evidence that DNA methylation plays an important role in this inhibition: (a) The mRNA level for DNA methyltransferase is highly increased in simian virus 40 (SV40)-transformed fibroblasts compared with normal cells and this increase correlates with the decrease of the mRNA level for collagen VI. (b) Methylation of the alpha2(VI) collagen promoter in vitro abolishes promoter activity in a transient transfection assay. (c) Genomic sequencing reveals extensive methylation of the promoter region in SV40-transformed cells, but virtually no methylation of the corresponding region in normal cells. Increased methylation is also observed in a rhabdomyosarcoma cell line. (d) Two of the cis-acting elements of the alpha2(VI) collagen promoter lose their affinity for transcription factor AP2 when methylated in vitro as demonstrated by gel retardation experiments. DNA methylation is therefore involved in the silencing of the alpha2(VI) collagen gene. It seems likely that the same mechanism is also responsible for the repression of other transformation-sensitive proteins.European Journal of Biochemistry 11/1997; 249(2):489-96. · 3.58 Impact Factor
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ABSTRACT: Descriptions for tenascin-C distribution are largely restricted to epithelial tumours. The present study utilized newly developed and characterized monoclonal (hT191) and polyclonal antibodies to investigate the distribution pattern of tenascin-C in a panel of mesenchymal tumours, which was contrasted with normal tissue. The specific antibodies recognized the distinctive star-like hexabrachion protein isolated from transformed cell-culture medium and serum from normal individuals. In normal tissues, a strong tenascin-C expression in the extracellular matrix was largely restricted to basement-membrane regions of epithelium and tonsilar sinusoids, pericellularly within smooth-muscle bundles, associated with perimysial, -chondrial, -neurial and -tendon surfaces, and diffusely within vascular adventitia. It was found in the corresponding tumours of the neural sheath (schwannoma) and smooth muscle (leiomyosarcoma), and was abundantly present around certain blood vessels of mesenchymal tumours. Although not detected in normal muscle, or in adipose or fibrous connective tissue, neo-expression of tenascin-C was shown in more than half of the rhabdomyosarcomas, fibromas and liposarcomas, with an increased positive percentage in variably malignant myxoid liposarcomas compared with lipoma-like sarcomas. Tenascin-C was typically found in the extracellular matrix of soft-tissue tumours, but was notably absent from the epithelial-cell components of mixed epithelial/mesenchymal tumours. Its apparently enhanced expression in soft-tissue tumours differs from that of most other large extracellular-matrix proteins, suggesting possible functional involvement of the cell-adhesion molecule, tenascin-C, in the neoplastic phenotype.International Journal of Cancer 08/1997; 72(2):217-24. · 6.20 Impact Factor
Article: t-Butylhydroperoxide and gliotoxin stimulate Ca2+ release from rat skeletal muscle mitochondria.[Show abstract] [Hide abstract]
ABSTRACT: Rat liver mitochondria have a specific Ca2+ release pathway which operates when NAD+ is hydrolysed to nicotinamide and ADPribose. NAD+ hydrolysis is Ca(2+)-dependent and inhibited by cyclosporine A (CSA). Mitochondrial Ca2+ release can be activated by the prooxidant t-butylhydroperoxide (tbh) or by gliotoxin (GT), a fungal metabolite of the epipolythiodioxopiperazine group. Tbh oxidizes NADH to NAD+ through an enzyme cascade consisting of glutathione peroxidase, glutathione reductase, and the energy linked transhydrogenase, whereas GT oxidizes some vicinal thiols to the disulfide form, a prerequisite for NAD+ hydrolysis. We report now that rat skeletal muscle mitochondria also contain a specific Ca2+ release pathway activated by both tbh and GT. Ca2+ release increases with the mitochondrial Ca2+ load, is completely inhibited in the presence of CSA, and is paralleled by pyridine nucleotide oxidation. In the presence of tbh and GT, mitochondria do not lose their membrane potential and do not swell, provided continuous release and re-uptake of Ca2+ ('Ca2+ cycling') is prevented. These data support the notion that both tbh- and GT-induced Ca2+ release are not the consequence of an unspecific increase of the inner membrane permeability ('pore' formation). Tbh induces Ca2+ release from rat skeletal muscle less efficiently than from liver mitochondria indicating that the coupling between tbh and NADH oxidation is much weaker in skeletal muscle mitochondria. This conclusion is corroborated by a much lower glutathione peroxidase activity in skeletal muscle than in liver mitochondria. The prooxidant-dependent pathway promotes, under drastic conditions (high mitochondrial Ca2+ loads and high tbh concentrations), Ca2+ release to about the same extent and rate as the Na+/Ca2+ exchanger. This renders the prooxidant-dependent pathway relevant in the pathophysiology of mitochondrial myopathies where its activation by an increased generation of reactive oxygen species probably results in excessive Ca2+ cycling and damage to mitochondria.Redox Report 01/1997; 3(5-6):331-41. · 1.66 Impact Factor
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ETH Zurich
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- • Institute of Biochemistry
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- Institute of Virology
Eawag: Das Wasserforschungs-Institut des ETH-BereichsDuebendorf, Zurich, Switzerland
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- Laboratoire de chimie et biochimie computationnelles
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The American Society for Biochemistry and Molecular BiologyRome, New York, United States
Washington University in St. LouisSan Luis, Missouri, United States
University of Washington Seattle
Seattle, Washington, United States
- Department of Medicine


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Last updated at 14:43, Friday, 06 June 2014
Trustees of a West Cumbrian charity, which was on the brink of collapse last year, have resigned.
Cumbria Rape Crisis was set up more than 20 years ago to help victims of rape and serious sexual crime.
Carol Tindall, of Flimby, has been the charity's chairwoman for 20 years.
She said new trustees, who have not yet been named, were due to take up their positions from tomorrow at the charity's annual meeting in the CVS offices on Selby Terrace, Maryport, at 10am.
She said: "There were various personal reasons for retiring but the main one was that most of us have been trustees for many years and it is time we made way for a new board."
The other trustees who have resigned are Paul Butler, of Harrington, Christine Ostle, of Embleton, and Kelly Pattinson, of Carlisle.
The charity hit a crisis point last October when it revealed that unless it could find emergency funding it would have to close its doors to the hundreds of victims which it supports every year.
A campaign raised more than £25,000 to keep it afloat and Miss Tindall said its future was looking more promising.
She said: "The establishment of a sexual assault referral centre in Penrith and an increased number of independent sexual violence advisors will certainly help its survival."
Miss Tindall, who has also been a counsellor for the charity, became involved with the group when she was invited to a meeting.
She added: "I was asked to advise them on finance and somehow ended up in the chair.
"On a personal level I will miss it deeply. I have worked with some wonderful volunteers over the years.
"This is a tough job and yet so many of the volunteers have remained with us for many years. I am grateful to them.
"You deal with some harrowing situations but thanks to our wonderful volunteers we have managed to help many of these victims.
"I wish the new trustees all the best for the future."
First published at 14:42, Friday, 06 June 2014
Published by http://www.timesandstar.co.uk
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Parry's NHL Hockey Power RankingFebruary 07, 2012 2:30 AM by Noland Parry
VIP | Noland Parry's NHL hockey Power Ranking is an excellent tool for gamblers to help calculate a number near what the anticipated point spread should be for wagering or betting.
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Bob Christ's breakdown of Pro Football Week 8 | Teams such as the Buccaneers that lose by 30-plus points the week before a bye, have come back strong their next game, going 8-0-1 ATS the past six seasons.
NCAA Football Betting Primer | The story of two teams heading in opposite directions as the Buckeyes have won four straight contests by the staggering average of 38.7 points per game, while the Nittany Lions have dropped their last two outings.
Trump Entertainment Resorts is dropping its threat to close Atlantic City's Taj Mahal casino on Nov. 13. The company said it still cannot guarantee the Taj Mahal won't close by the end of the year, but said it will remain open throughout November.
Bob Christ's Thursday NFL Football Breakdown | When Denver plays host to San Diego on Thursday night in Week 8, Denver QB Peyton Manning will be going against one of the few teams that packs kryptonite when it faces the record-setting quarterback.
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Aloha there at our free ringtones website, where you can use killer ringtones for your smart phone. Today we have a ringtone of "You Are Not Alone" by Michael Jackson as well as 7499 other ringtones by Michael Jackson. "You Are Not Alone" is a hit from the "Not Guilty (Special Fan Edition)" album that was recorded and mixed by Michael Jackson at their major label together with other 24 tracks. You can listen to all 176 Michael Jackson – "You Are Not Alone" ringtone variations available here and download it for free directly to your mobile. You can also select other 34 albums produced by Michael Jackson for all time. "You Are Not Alone" is referred to Pop, and you can download a huge amount of Pop albums absolutely for free. And finally, we have collected lyrics for this track and you can view it here as well. So feel free to browse, listen, download ringtones at Audiko.net. Make your phone sound cool!
Michael Jackson - You Are Not Alone
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You Are Not Alone 5:34
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Tel: (904)879-1260
Grades: NS - 12 | 170 students
Our mission is to lead each student to the saving knowledge of Jesus Christ by consistently teaching the Word of God while providing personalized academic excellence.
In accomplishing our mission, we focus on the following priorities: � Seek first the kingdom of God and his righteousness in accordance with Matthew 6:33.
� Strive as a teaching staff to meet the highest standards of professionalism and Christian example in our instruction.
� Provide Christ centered curricula to ensure sound academic achievement in all areas of study.
� Maintain accreditation through Florida League of Christian Schools (FLOCS) for grades Kindergarten - 12th, to ensure excellence in academic requirements.
� Accurately assess the needs of individual students; and � Partner with your family to achieve your student's success.
Understanding the importance of commitment, communication, and teamwork, I look forward to ministering to your family this school year.
School Overview
|School Membership(s)School Assoc.
|Association of Christian Teachers and Schools (ACTS)
State or regional independent school association
Other religious school association(s)
|Religious Affiliation
|Assembly of God
|Grades Offered
|Grades Nursery/Preschool - 12
|Learning Difference Programs
|Learning Programs Supported
|Mild Learning Differences
|Year Founded
Student Body
|Total Students
|170 students
|Student Body Type
|% Students of Color
State avg.: 32%
|Students by Grade
Academics and Faculty
|Total Classroom Teachers
|35 teachers
|Student : Teacher Ratio
National avg.: 13:1
|% Faculty w/Advanced Degree
|Average Class Size
|16 students
|Classroom Dress Code
Finances and Admission
|Admission Deadline
|None / Rolling
|Yearly Tuition Cost
|Tuition Notes
|Tution for Kindergarten $3750 Tution for 1st - 6th $4000 Tuition for 7th - 11th $4200
|Acceptance Rate
National avg.: 85%
|Admissions Director
|Lorie Johnson
|Admissions Associate
|Shelly DeLee
|Total Sports Offered
|3 sports
|Basketball, Cheering, SoftballBasketball, Cheering,
|Total ExtracurricularsTotal Extra-curric.
|6 extracurriculars
|4H Program, Beginning Concert Band, Fine Arts, Intermediate Concert Band, Karate, Praise band 4H Program, Fine Arts, Karate, Praise band, Beginning Concert Band,
Intermediate Concert Band,
School Notes:
- We accept applications on a first-come, first-serve basis. Feel free to contact our office or check our website at sonshinechristian.com should you have questions. Office hours are Monday through Friday from 7:30 a.m. to 4:00 p.m.
Profile last updated: 09/02/2014
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Callahan Nassau County Priv School Syst
Callahan , FL , 32011
(904)225-5265Alternative School8.9 mi | 37 students | Gr. PK-12
Kingsland Advance Learning Center
Po Box 2598
Kingsland , GA , 31548
(912)882-5327(Christian)11.3 mi | 22 students | Gr. 2-12
Folkston Charlton Christian Academy
Folkston , GA , 31537
(912)496-4120(Christian)16.5 mi | 21 students | Gr. 1-12
Jacksonville Hope Chapel Christian Assembly
9850 Wagner Rd
Jacksonville , FL , 32219
(904)924-2000(Christian)17.5 mi | 138 students | Gr. K-12
JacksonvilleEsprit De Corps Center For Learning
9840 Wagner Rd
Jacksonville , FL , 32219
(904)924-2000(Christian)17.5 mi | 154 students | Gr. KG-12
School Reviews
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Updated October 14, 2014
Add things up and you'll quickly find the answer- math is crucial both in academics and the real world. By following these strategies and following personal training programs, students can uncover their weaknesses and conquer math.
"Because I heard it is a good school." That may well be, but there are some other factors in the private school selection process we need to consider.
Running a small to medium sized private school? Can't afford marketing staff and expensive marketing programs? Read on.


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Waste Management of the Pacific Northwest (WM) has entered into an agreement to acquire Glacier Recycle
Waste Management of the Pacific Northwest (WM) has entered into an agreement to acquire Glacier Recycle, an Auburn, Wash.-based company that provides construction-related recycling.
In a press release, WM officials say the acquisition will expand the company's "green services" related to waste reduction, waste recovery and the processing of waste into new materials.
Glacier, a construction and demolition recycling firm, recycles and processes recovered wood for the manufacturing of recycled wood products and for use as biomass fuel. The company serves the entire Puget Sound area.
Texas group launches statewide recycling study Friday, October 24, 2014 05:32:35 PM
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Join us in November Tuesday, October 21, 2014 05:20:07 PM
Goodyear's global headquarters earns LEED Gold rating Monday, October 20, 2014 06:06:38 PM
Bayshore Recycling wins New Jersey conservation award Friday, October 17, 2014 03:10:05 PM
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Never-occupied Las Vegas casino to be demolished Tuesday, October 14, 2014 03:55:12 PM
National Demolition Association changes management Tuesday, October 14, 2014 03:55:05 PM
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5811 Canal Road
Valley View, OH 44125 USA


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Jump to content
wen1 hasn't added any friends yet.
13 September 2014 - 10:07 AM
are you using mini display to hdmi or vga?
are you using mini display to hdmi or vga?
mini display to HDMI cable
10 September 2014 - 10:21 PM
I am definitely having issues with sleep. Not sure if it is due to the platform id i am using or the wireless card.
09 September 2014 - 01:19 AM
Got it,
May be you guys will have better luck with it. I will try the sleep to see how that works now with this. I did not see any issues with the Yosemite version of the kext. But it is too soon for me to say though.
08 September 2014 - 12:46 PM
Just to note you will also need a tweak to the DSDT and the config.plist as i mentioned above to inject 0x01660009. I did not post those two file to avoid confusion.
I sure can send them to you if needed.
07 September 2014 - 11:55 PM
I got the display port working by patching the AppleIntelFrameBufferCapri.kext manually. Now it detects the external monitor properly and supports full resolution via that port. Also detects audio port (verified via DPCIManager, but did not test actual sound output).
The HD4000 card in surface has the revision id of 0x0009. So i modified the kext above for that platform id to support the LVDS in surface pro and modified the DSDT and the config file to inject 0x01660009 instead of 0x01660004.
Let me know if there is any interest and i can post the the files used.


text:
Cruise News
Holland America Line adds new vegetarian-only menu
Holland America Line has introduced a new alternative, vegetarian-only menu, as well as 30 new vegetarian dishes to their main dining room menu.
Its ward-winning Master Chef Rudi Sodamin has designed an exclusive 22-dish vegetarian and vegan menu that highlights vegetables and other naturally healthy ingredients.
The alternative menu includes a full range of appetisers, salads, soups and main entrees, and is available upon request at no extra cost for lunch and dinner in the main dining room aboard all 15 ships in the fleet.
"Many more people are choosing a vegetarian culinary experience, yet the options can be limited," said Holland America's executive vice president Richard Meadows.
"By offering a complete vegetarian-only menu and the largest vegetarian selection at sea, we are ensuring that all guests can take part in an exceptional dining experience while cruising with Holland America Line."
In addition to the new vegetarian-only menu, 30 new vegetarian dishes will be added to the main dining room menu. These offerings also will be featured as a second vegetarian option in the casual Lido buffet during lunch. Additionally, every dinner menu will now offer guests the option of one appetiser, one soup or salad, and at least one vegetarian entrée each evening, increasing the total number of vegetarian options on board to 52 dishes.
Special Offers More..
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25 October 2014
Cruise on Minerva from the mesmerising shores of the Red Sea to the mystical Far East and save up to… More...
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Put a holiday cruise with Holland America Line on your gift list this season and you just might get your… More...
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15 October 2014
Holland America Line heads to the tropics of Hawaii and explores the spirit of Mexico from fall 2014 through spring… More...
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The Leading eBooks Store Online
for Kindle Fire, Apple, Android, Nook, Kobo, PC, Mac, BlackBerry ...
Precarious Work, Women, and the New Economy
The Challenge to Legal Norms
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moreGlobalisation, the shift from manufacturing to services as a source of employment, and the spread of information-based systems and technologies have given birth to a new economy, which emphasises flexibility in the labour market and in employment relations. These changes have led to the erosion of the standard (industrial) employment relationship and an increase in precarious work - work which is poorly paid and insecure. Women perform a disproportionate amount of precarious work. This collection of original essays by leading scholars on labour law and women's work explores the relationship between precarious work and gender, and evaluates the extent to which the growth and spread of precarious work challenges traditional norms of labour law and conventional forms of legal regulation.The book provides a comparative perspective by furnishing case studies from Australia, Canada, the Netherlands, Quebec, Sweden, the UK, and the US, as well as the international and supranational context through essays that focus on the IMF, the ILO, and the EU. Common themes and concepts thread throughout the essays, which grapple with the legal and public policy challenges posed by women's precarious work. less
Hart Publishing Limited; Read online
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Lean In 2013 US$ 12.99 208 pages
Unequal Childhoods 2003 US$ 15.95 345 pages
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Jazzhole - Free The Robots
Jazzhole - Free The Robots
dunskdunskdunskall that jaaaaazzzzzzzzz
lupe fiascommhm, mmhm
Binary Star | Reality Check
one of my favorites :)
Finally some down time to listen to their new album.And so far, I like it :)Deep music right hurrrr
kill it with the beat.
yeah the lyrics are a bit.., but beat is sick.
lovin the jazz guitar/piano kick in this song
You give your shoulders for others to lean on to, but you find yourself shoulder-less and fall in silence.
{With Love.}
Atmosphere - Sunshine
Atmosphere - Sunshine
sunshine, sunshine, it's fineI feel it in my skin, warming up my mind,Sometimes you gotta give in to win,I love the days when it shines, whoa let it shine
With this lovely weather, it's spring on the brink of summer