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News: Diddy's Lil Wayne Jail Visit Confirmed, "No One Knew [He Was Here] Until It Was Time For Him To Leave"
Friday, May 14, 2010 10:30AM
Diddy's recent visit to see Lil Wayne at Rikers Island jail has been confirmed by an inside source.
According to a source, Diddy's presence at the facility was not highly publicized.
"No one knew [he was here] until it was time for him to leave," the source told MTV News. "You know, also, a lot of the time people don't want to seem like they're stargazers, the officers." Diddy has yet to comment about his Lil Wayne visit. "It's just day-to-day business, nothing different than anything else," the source said of the New Orleans MC's activities. "You got to understand, this is a different type of atmosphere. It's not like the prisons where you go upstate, to Sing Sing or places like that. This is a medium-security [population], not maximum security." (MTV)
Diddy reportedly stopped by to see Wayne in late April.
Rap mega-star Lil Wayne - locked up on a gun conviction - brought in at least one more bold-faced name this week: Diddy. The music mogul stopped by the Eric M. Taylor correctional facility to visit Lil Wayne on Wednesday. Diddy, born Sean Combs, even swapped his luxury wheels for the public bus like all the other visitors Wednesday. Diddy was on a pre-approved list to see Lil Wayne, whose real name is Dwayne Carter Jr., and the chat was limited to an hour, sources said. (New York Daily News)
Rapper T.I. recently paid homage to Wayne at a celebration ceremony in California.
T.I.'s welcome-home party was nothing short of epic - the infamous rapper was feted by the likes of Jamie Foxx, Usher and Diddy at L.A. hot spot Guys and Dolls on Sunday night, and the evening turned into a toast to the singer's future. Others onhand to welcome T.I. back on the scene included Lil' Kim, rapper Rick Ross and Amber Rose, who arrived sans Kanye West and almost sans clothing - she wore nothing but a nude leotard. "I'm so happy to be with all of you tonight," he said. "And I cannot thank you enough for being here." Not just for T.I.: The rapper took some of the spotlight off himself when he asked the room to acknowledge another wayward star. "There are still people in the hip-hop community that need our support," he said. "So I ask everybody to please raise your glasses for Lil Wayne." (New York Daily News)
Wayne was sentenced to a year behind bars last March.
Lil Wayne has been sentenced to a year in jail after pleading guilty in a New York City gun case. The Grammy Award-winning rapper was taken away in handcuffs to start serving his term immediately after his sentencing. He'll serve his sentence in city jails, not a state prison. He could be released in about eight months with good behavior. (CBS News)
Check out a pre-jail Lil Wayne interview down below:


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This article originally appeared in our September 1980 issue.
"On Auburn Avenue, we expect to restore his birthplace and continue the life of Ebenezer Baptist Church, his spiritual home. Together with these, in a memorial park, we plan to locate his final place of entombment and build as well a living, open Freedom Hall which will tell for many generations the story of the movement which he led."
-Coretta Scott King
January 15, 1969
Since Mrs. King uttered that statement over 11 years ago, the Martin Luther King, Jr. Center for Social Change has had many vocal critics, some in Atlanta, others nationwide.
Boyd Lewis, a reporter for Radio Station WABE in Atlanta, has been among the most critical and caustic. In his probing investigative reports, also carried on the national public radio network, Lewis questioned what happened to $5 million in grants which have come to the Center during the past 11 years; why it took ten years for the organization to file an annual fiscal report with the State of Georgia; the function of the Fund for Peace, Nonviolence and Brotherhood, which received a total of $362,191 from the Center from 1971 to 1977; and the fact that the chairman of the board of trustees didn't know of the existence of this fund.
Lewis also asked why Jimmy Carter was given the Martin Luther King, Jr. Peace Prize in 1979, implying it was a politically motivated gesture to help the Center receive large sums of money from a variety of Federal agencies. Why did the Southern Christian Leadership Conference which was founded by Dr. King, picket the 1979 MLK birthday week activities?
What, exactly, is the Martin Luther King, Jr. Center for Social Change?
Mrs. King granted ATLANTA Magazine a four-hour interview when the initial research into these questions began, and other Center staff members were equally helpful. Board members were interviewed, along with former directors in an attempt to unravel some of the mystery often associated with what the Center is doing.
To investigate allegations of financial mismanagement and possible violations of the regulations for solicitation and collection of funds for charitable purposes, interviews were held with officials in the Georgia Secretary of State's Office and with officials in the Office of the Attorney General. Atlanta Constitution reporters who had written a series of articles on the Center in 1979 were questioned, and the publisher of a black newspaper in Atlanta — a critic of what the Center has and has not been doing — was interviewed.
In short, opinions and facts were gathered from friend and foe alike in an effort to gather sufficient information to objectively analyze the organization and its success or failure in reaching its stated goals and objectives.
One conclusion comes across loud and clear after interviewing scores of individuals: there is a great deal of general confusion about the real purpose of the Center.
John Cox, an active board member, chairperson of the steering committee for King Week 1980, and a Delta Air Lines executive, says the Center is "a Mecca for black people. It symbolizes a great person, a great man.'' He further states that "it is a national organization," and that it has "a national and an international mission." It is not a local center, he says. "It is manifested in Atlanta and in other parts of the country.
Some, however, feel good works should begin at home.
C. A. Scott, owner and publisher of The Atlanta Voice, believes the King Center should be doing more in Atlanta to help the elderly and the needy in emergency situations. He also believes the mission of the Center should be primarily to "carry out the basic non-violent philosophy of Dr. King." Mr. Scott, an active Republican, also says Mrs. King is too partisan to the Democratic Party and President Carter. (Mrs. King, Dr. Martin Luther King, Sr., and most of the King family have publicly endorsed Carter for President in the 1980 election.)
Mrs. King considers her personal work and efforts as being vital parts of the contribution the King Center is making, and it is difficult to separate the activities of the Center from her activities. From 1971 to 1977, $362,191 was taken from the treasury of the Center and "donated" to the MLK Fund for Peace and Non-Violence. The address for this Fund was Mrs. King's home, and there is no public accounting for how these funds were spent. Official King Center records indicate Mrs. King received a salary of only one dollar per year from 1968 through 1978. Her annual stipend as of October, 1979, is $12,000 per year.
Mrs. Christine Farris, treasurer of the Center and sister of the slain Nobel Prize winner, says the Fund was used to pay for the Center's non-profit business affairs. (The Fund has since been succeeded by the President's Program.)
In 1978 Mrs. King spent $80,000 to attend conferences and do whatever she felt was necessary to accomplish the goals of the Center. Nonviolent Change, an occasional newsletter put out by the Center, noted in its Winter, 1979, edition that the President's Program "includes Mrs. King's work as she travels across the country speaking out on social change issues as Chief Executive of the King Center." (In 1979 her work also took her to Europe and Asia.)
Lloyd Davis, the new Executive Vice President and Chief Operating Officer of the Center, agrees with Mrs. King that her efforts have been crucial in helping the organization move toward meeting its goals and objectives. He says the nation would not now have the Humphrey-Hawkins full-employment bill had it not been for the King Center and the personal efforts of Mrs. King and other officers in helping persuade key people in Washington to support the bill. Davis also says we would not have other pieces of social-oriented legislation without the efforts which have been put forth through the Center's President's Program. He cites the White House Conference on Families (on which Mrs. King was the deputy chairperson) and support of the Equal Rights Amendment and causes of the labor movement as examples of important and meaningful activities of the King Center and Mrs. King.
Some argue Mrs. King could have participated in all of these activities as an individual even if there were no such entity as the MLK Center for Social Change. Her stature as the widow of Dr. King might easily qualify her to sit on numerous boards and commissions. Supporters contend her position as President of an organization dedicated to social change gives her the opportunity to establish her own identity and reputation as an effective leader and organizer, fighting for issues and causes she believes are just and right.
An Atlanta Constitution series on the King Center, published October 17 and 18, 1979, by Carole Ashkinaze, Seth Kantor, Jim Stewart and Tina McElroy, delineated the Center's ability to raise millions of dollars during the past 11 years for its many projects and activities. Steve Klein, who is in charge of the Center's pubic relations, says he did not consider the series an objective evaluation of the entire organization, although he did admit the King Center generally got better coverage from the Constitution and the Atlanta Journal than from most newspapers.
In an effort to investigate allegations that the organization might be raising funds for one purpose and using them for another, ATLANTA Magazine examined certain reports which have been recorded with the Secretary of State's Office. According to the state law which regulates professional fund raisers, every charitable organization which has received contributions during the preceding calendar year "shall file a written report with the department [Department of State] on or before March 31st of each year . . . " The Center for Social Change, which began receiving funds in 1971, did not file until 1979, after WABE's Boyd Lewis discovered the Center had never filed a financial report in Georgia.
A careful review of the reports filed since 1979 by this reporter and Boyd Lewis found no evidence of a misappropriation of funds. Officials and board members state their lawyers had not known they had to file a report in Georgia.
But what have they been doing with all that money?
Next Page: A center out of touch with its community

subset: commoncrawl


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Communicators with David Ensor
Director of the Voice of America (VOA) David Ensor discussed changes in the U.S. government-funded national and international news and… read more
Director of the Voice of America (VOA) David Ensor discussed changes in the U.S. government-funded national and international news and information network.
This week's "The Communicators" is the first in a series about U.S. Government-sponsored broadcasts to other countries. These broadcasts are services of the Broadcasting Board of Governors. close
*The transcript for this program was compiled from uncorrected Closed Captioning.
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- Ensor, David Director Voice of America
- Hagey, Keach Correspondent Politico
- Slen, Peter Host C-SPAN
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Two years after an ordinance was passed to shut down bars early in Fat City, more businesses in the area have closed. Business owners agree the changes to Fat City are obvious, but some don't think they're for the better.
"Look at the building for sale right here, you know. 3018... all of these places are just gone," said Boom Boom's bar owner Barbara Ann Richardson.
Richardson sees a dying Fat City. She's now relying on a loyal customer base to stay open.
"It's a shame, that this has to be done," Richardson says. "I know they were trying to get rid of some undesirable elements, but it has hurt everybody,"
Popular Italian restaurant Salvatore's, which relied heavily on its late-night crowd and its Latin nights for the bulk of its revenue, closed in January. "It really hurt him," said Richardson.
Area business owners say the building is now being leased.
"I don't understand how they can just take one little section, of the whole state, and say you all have to close at midnight," said Richardson.
Last September, Salvatore's owner Saul Bollat, who said the new ordinance limiting bar hours to 12:00 a.m. on weeknights and 1:00 a.m. on weekends, was killing his business.
"I kept it open because of the restaurant, because I'm a chef. I went from a million dollars in sales to a half-a-million dollars in sales because of smart ideas," said Bollat.
Now, like many other bar and restaurant owners such as City Bar and the Edge, Bollat has moved on.
"Certainly for bars, it probably hurt their business, although I'm glad that some bars are still there and are still able to operate. So Fat City still has bars, we just have that curfew," said Jefferson Parish councilwoman Cynthia Lee-Sheng.
Lee-Sheng has led the fight to shut down bars early in Fat City. The ordinance passed in September 2010. Two years later, she says she continues to see victories in what she believes will be a 10-to-12 year transition for the area.
"And we do have some new businesses going there and we still have other businesses looking at some places there, so we're really moving forward in the right direction," said Lee-Sheng.
"I think Cynthia thinks she can do some things to improve Fat City, and I hope she can," said Richardson.
Lee-Sheng says all adult establishments and strip clubs are set to close by December 31. It was all part of the ordinances passed in 2010.
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New Orleans -- Jefferson Parish Sheriff's Department Homicide Detectives are investigating a shooting that left woman dead. The incident occurred Saturday around 9:24 p.m. in the 1000 block of Robinson Avenue in Marrero. The victim has been identified as 19-year-old Miquisha Nash of New Orleans.
Police says that responding officers arrived on scene to find Nash sitting in the passenger seat of a car, suffering from multiple gunshot wounds. She was transported to a local hospital where she was pronounced dead shortly after arrival.
Investigators learned that Nash traveling in the car in the 5400 block of Westbank Expressway, with her friend, when a van pulled along side of them and began firing. When the friend became aware of the gunfire, he put the car in reverse and sped away from the van, before stopping on Robinson Avenue to wait for police and EMS.
Detectives are working to determine a motive and find the person(s) responsible. If you have any information that could help investigators, you are asked to call CRIMESTOPPERS at 504-822-1111 or Detective Francis at 504-364-5300.
Fox 8 WVUE-TV
Louisiana Media Company, LLC.
1025 S. Jefferson Davis Parkway
New Orleans, LA 70125
General Number: (504) 486-6161
News Tips: (504) 483-1503
News Room Fax: (504) 483-1543


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Research Topics
Genomes and Genes
carbamyl phosphate
Summary: Phosphate salt of carbamic acid.
Top Publications
Research Grants
Scientific Experts
Detail Information
Publications184 found, 100 shown here
- Catalytic domains of carbamyl phosphate synthetase. Glutamine-hydrolyzing site of Escherichia coli carbamyl phosphate synthetaseS D Rubino
J Biol Chem 261:11320-7. 1986We present evidence that cysteine 269 of the small subunit of Escherichia coli carbamyl phosphate synthetase is essential for the hydrolysis of glutamine...
- Crystal structure of human ornithine transcarbamylase complexed with carbamoyl phosphate and L-norvaline at 1.9 A resolutionD Shi
Department of Biochemistry, University of Minnesota, St Paul, Minnesota, USA
Proteins 39:271-7. 2000..The structures of the active sites in the complexes of aspartate transcarbamylase (ATCase) with various substrates or inhibitors are similar to this OTCase structure, consistent with their common evolutionary origin...
- Metabolic channeling of carbamoyl phosphate, a thermolabile intermediate: evidence for physical interaction between carbamate kinase-like carbamoyl-phosphate synthetase and ornithine carbamoyltransferase from the hyperthermophile Pyrococcus furiosusJan Massant
Department of Microbiology, Vrije Universiteit Brussel, Flanders Interuniversity Institute for Biotechnology, Universite Libre de Bruxelles, Belgium
J Biol Chem 277:18517-22. 2002..Therefore, by physically interacting with each other, CKase and OTCase prevent the thermodenaturation of carbamoyl phosphate in the aqueous cytoplasmic environment...
- Structural model of the R state of Escherichia coli aspartate transcarbamoylase with substrates boundJie Wang
Department of Chemistry, Boston College, Merkert Chemistry Center, Chestnut Hill, MA 02467, USA
J Mol Biol 371:1261-73. 2007..By combining these data with previously determined structures we can now visualize the allosteric transition through detailed atomic motions that underlie the molecular mechanism...
- The crystal structures of ornithine carbamoyltransferase from Mycobacterium tuberculosis and its ternary complex with carbamoyl phosphate and L-norvaline reveal the enzyme's catalytic mechanismRamasamy Sankaranarayanan
Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
J Mol Biol 375:1052-63. 2008..We have interpreted this deprotonation as part of the collapse of the transition state of the reaction...
- Insights into the mechanism and regulation of pyruvate carboxylase by characterisation of a biotin-deficient mutant of the Bacillus thermodenitrificans enzymeAbdussalam Adina-Zada
School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Int J Biochem Cell Biol 40:1743-52. 2008..Attempts to trap the putative carboxyphosphate intermediate in K1112A using diazomethane were unsuccessful...
- Molecular physiology of phosphoryl group transfer from carbamoyl phosphate by a hyperthermophilic enzyme at low temperatureSantiago Ramon-Maiques
Instituto de Biomedicina de Valencia CSIC, Jaume Roig 11, 46010 Valencia, Spain
Biochemistry 41:3916-24. 2002..Probably the catalysis of the phosphoryl group transfer is less efficient at low temperature, as suggested by structural data showing that Lys131 is improperly positioned to assist the transfer...
- A functional analysis of the allosteric nucleotide monophosphate binding site of carbamoyl phosphate synthetaseOlivier A Pierrat
Department of Chemistry, Texas A and M University, College Station, Texas, 77842 3012
Arch Biochem Biophys 400:34-42. 2002..With the exception of V994A, these mutations had a modest influence on the binding and subsequent allosteric effects by UMP and IMP...
- A 70-amino acid zinc-binding polypeptide fragment from the regulatory chain of aspartate transcarbamoylase causes marked changes in the kinetic mechanism of the catalytic trimerB B Zhou
Department of Molecular and Cell Biology, University of California, Berkeley 94720
Protein Sci 3:967-74. 1994..The rate of dissociation of carbamoyl phosphate from the Zn domain-C trimer complex was about 10(-2) that from C trimer.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Kinetics of the quaternary structure change of aspartate transcarbamylase triggered by succinate, a competitive inhibitorH Tsuruta
Stanford Synchrotron Radiation Laboratory, SLAC, California 94309 0210
Biochemistry 33:10007-12. 1994..This is consistent with a concerted structural transition of ATCase.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Apparent cooperativity for carbamoylphosphate in Escherichia coli aspartate transcarbamoylase only reflects cooperativity for aspartateP England
Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, URA CNRS 1682, Universite Pierre et Marie Curie, Paris, France
Eur J Biochem 222:775-80. 1994....
- Ligation alters the pathway of urea-induced denaturation of the catalytic trimer of Escherichia coli aspartate transcarbamylaseS Bromberg
Department of Biochemistry, University of Minnesota, St Paul 55108
Protein Sci 3:1236-44. 1994..c3) of aspartate transcarbamylase from Escherichia coli at low temperature in the absence and presence of carbamyl phosphate (CP; a substrate), N-(phosphonacetyl)-L-Asp (PALA; a bisubstrate analog), and 2 anionic inhibitors, Cl- and ..
- Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycleMelanie Blokesch
Microbiology, Department I Biology, University of Munich, Maria Ward Strasse 1a, D 80638 Munich, Germany
J Mol Biol 324:287-96. 2002..On the basis of the results, a cycle of HypC activity is proposed whose function is to transfer the iron atom that has been liganded at the HypC-HypD complex to the precursor of the large hydrogenase subunit...
- Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutrophaOliver Lenz
Humboldt Universitat zu Berlin, Institut für Biologie Mikrobiologie, Chausseestrasse 117, Berlin, Germany
FEBS Lett 581:3322-6. 2007..Incorporation of label from (13)CO exclusively into the carbonyl ligand indicates that free CO is a possible precursor in carbonyl ligand biosynthesis...
- Deconstruction of the catalytic array within the amidotransferase subunit of carbamoyl phosphate synthetaseX Huang
Department of Chemistry, Texas A and M University, College Station, Texas, 77843, USA
Biochemistry 38:15909-14. 1999..It is concluded that contrary to the original proposal for the catalytic triad, Glu-355 is not an essential residue for catalysis. The results are consistent with Ser-47 and Gln-273 playing significant roles in the binding of glutamine...
- Acetylornithine transcarbamylase: a novel enzyme in arginine biosynthesisHiroki Morizono
Children s Research Institute, Children s National Medical Center, 111 Michigan Avenue NW, Washington, DC 20010, USA
J Bacteriol 188:2974-82. 2006..coli and show that it catalyzes the formation of N-acetyl-L-citrulline from N-acetyl-L-ornithine and carbamyl phosphate. We now designate this enzyme as an acetylornithine transcarbamylase...
- The 1.5 A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insight inS Ramon-Maiques
Consejo Superior de Investigaciones Cientificas IBV CSIC, Instituto de Biomedicina de Valencia, C Jaime Roig 11, Valencia, 46010, Spain
J Mol Biol 299:463-76. 2000..The structure provides the first information on substrate binding and catalysis in CKs, and suggests that the slow rate at 37 degrees C is possibly a consequence of slow product dissociation...
- Perforation of the tunnel wall in carbamoyl phosphate synthetase derails the passage of ammonia between sequential active sitesJungwook Kim
Department of Chemistry, Texas A and M University, P O Box 30012, College Station, Texas 77842 3012, USA
Biochemistry 43:5334-40. 2004..Overall, the catalytic constants of the mutant were consistent with the model where the channeling of ammonia has been disrupted due to the leakage from the ammonia tunnel to the protein exterior...
- Half of Saccharomyces cerevisiae carbamoyl phosphate synthetase produces and channels carbamoyl phosphate to the fused aspartate transcarbamoylase domainV Serre
Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, UMR 7631 CNRS Université Pierre et Marie Curie, 96 Bd Raspail 75006 Paris, France
J Biol Chem 274:23794-801. 1999....
- Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal centerJ B Thoden
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Biochemistry 40:6989-97. 2001..The ensuing tetrahedral intermediate collapses with C-O bond cleavage and expulsion of the hydroxide which then bridges the binuclear metal center...
- Carbamate kinase: New structural machinery for making carbamoyl phosphate, the common precursor of pyrimidines and arginineA Marina
Instituto de Biomedicina de Valencia CSIC, Spain
Protein Sci 8:934-40. 1999..The orientation of the crevices in the dimer precludes their physical cooperation in the catalytic process. Such cooperation is not needed in the CK reaction but is a requirement of the mechanism of CPSs...
- The structure of carbamoyl phosphate synthetase determined to 2.1 A resolutionJ B Thoden
Institute for Enzyme Research, The Graduate School, and Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53705, USA
Acta Crystallogr D Biol Crystallogr 55:8-24. 1999..The carboxyphosphate and carbamoyl phosphate synthetic components, while topologically equivalent, are structurally different, as would be expected in light of their separate biochemical functions...
- cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding siteY Lee
School of Biological Sciences, Seoul National University, Korea
Plant Mol Biol 46:651-60. 2001..It is assumed that the substrate specificity of ClOCT1 or ClOCT2 proteins partially depends on the amino acid sequence of the CP-binding site...
- Divergent polyamine metabolism in the ApicomplexaTuesday Cook
Haskins Laboratories, Pace University, New York, NY 10038, USA
Microbiology 153:1123-30. 2007..The lack of polyamine biosynthesis by T. gondii is contrasted with polyamine metabolism by other apicomplexans...
- Direct observation of an altered quaternary-structure transition in a mutant aspartate transcarbamoylaseH Tsuruta
Stanford Synchrotron Radiation Laboratory, California, USA
Proteins 31:383-90. 1998..They also illustrate the crucial role played by domain motions and quaternary-structure changes for both the homotropic and heterotropic aspects of allostery...
- Tertiary and quaternary conformational changes in aspartate transcarbamylase: a normal mode studyA Thomas
Laboratoire de Dynamique Moleculaire, Institut de Biologie Structurale Jean Pierre Ebel, Grenoble, France
Proteins 34:96-112. 1999..The particular dynamic behavior of these residues is examined in the light of biochemical results to obtain insights into their role in the transmission of the allosteric signal...
- Isolation and partial characterization of an argR mutant of Salmonella typhimuriumR A Kelln
J Bacteriol 128:528-35. 1976..Ingraham, Abstr. Annu. Meet. Am. Soc. Microbiol. 1975, K169, p. 175) have shown constitutive synthesis of carbamyl phosphate synthetase in the argR mutant, the regulation of the synthesis of the last five enzymes of the pyrimidine ..
- Importance of ornithine transcarbamylase (OTC) deficiency in small intestine for urinary orotic acid excretion: analysis of OTC-deficient spf-ash mice with OTC transgeneT Saheki
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan
Biochim Biophys Acta 1270:87-93. 1995..These results suggest that the level of OTC activity in the small intestine is important for production of orotic acid...
- Extent of genetic lesions of the arginine and pyrimidine biosynthetic pathways in Lactobacillus plantarum, L. paraplantarum, L. pentosus, and L. casei: prevalence of CO(2)-dependent auxotrophs and characterization of deficient arg genes in L. plantarumFrançoise Bringel
Laboratoire de Dynamique, Expression et Evolution des génomes de micro organismes, FRE 2326 Université Louis Pasteur CNRS, 28 rue Goethe, 67083 Strasbourg, France
Appl Environ Microbiol 69:2674-83. 2003..These HCR auxotrophs may have had their CP cellular pool-related genes altered or deregulated...
- Taming of a poison: biosynthesis of the NiFe-hydrogenase cyanide ligandsStefanie Reissmann
Department Biologie I, Mikrobiologie, University of Munich, Maria Ward Strasse 1a, D 80638 Munich, Germany
Science 299:1067-70. 2003..This finding underscores a striking parallel between biochemistry and organometallic chemistry in the formation of an iron-cyano complex...
- Site-directed mutagenesis of Arg60 and Cys271 in ornithine transcarbamylase from rat liverS McDowall
Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia
Protein Eng 4:73-7. 1990..The small loss in binding energy and relatively high residual catalytic activity of the mutant strongly suggests that a number of other residues are involved in the binding of ornithine.(ABSTRACT TRUNCATED AT 250 WORDS)..
- New experimental approaches for investigating interactions between Pyrococcus furiosus carbamate kinase and carbamoyltransferases, enzymes involved in the channeling of thermolabile carbamoyl phosphateJan Massant
Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
Archaea 1:365-73. 2005....
- Metabolic channelling of carbamoyl phosphate in the hyperthermophilic archaeon Pyrococcus furiosus: dynamic enzyme-enzyme interactions involved in the formation of the channelling complexJ Massant
Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
Biochem Soc Trans 32:306-9. 2004....
- In Pseudomonas syringae pv. phaseolicola, expression of the argK gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase, is regulated indirectly by temperature and directly by a precursor resembling carbamoylphosphateKarina López-López
Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados CINVESTAV del Instituto Politecnico Nacional IPN, Unidad Irapuato, Irapuato, Guanajuato, C P 36500, Mexico
J Bacteriol 186:146-53. 2004..These results indicate that argK expression is independent of temperature and is regulated directly by a compound resembling the inorganic moiety of phaseolotoxin...
- Kinetic characterization of yeast pyruvate carboxylase isozyme Pyc1 and the Pyc1 mutant, C249AJoy P Branson
Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Biochemistry 43:1075-81. 2004....
- Three-dimensional structure of carbamoyl phosphate and succinate bound to aspartate carbamoyltransferaseJ E Gouaux
Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138
Proc Natl Acad Sci U S A 85:4205-8. 1988..In comparing the structure of the active sites in the PALA-enzyme complex to the active sites in the carbamoyl phosphate-succinate-enzyme complex, we find that they are similar...
- Regulatory changes in the control of carbamoyl phosphate synthetase induced by truncation and mutagenesis of the allosteric binding domainR M Czerwinski
Department of Chemistry, Texas A and M University, College Station 77843, USA
Biochemistry 34:13920-7. 1995..Six conserved amino acids within the allosteric domain were mutated.(ABSTRACT TRUNCATED AT 250 WORDS)..
- HypF, a carbamoyl phosphate-converting enzyme involved in [NiFe] hydrogenase maturationAthanasios Paschos
Mikrobiologie, Department I der Fakultät für Biologie, Ludwig Maximilians Universitat Munchen, Maria Ward Strasse 1a, D 80638 Munich, Germany
J Biol Chem 277:49945-51. 2002..The results are discussed in terms of the involvement of HypF in the conversion of carbamoyl phosphate to the CN ligand...
- T-state active site of aspartate transcarbamylase: crystal structure of the carbamyl phosphate and L-alanosine ligated enzymeJingwei Huang
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Biochemistry 45:346-52. 2006..2 A) when ATCase is bound to carbamyl phosphate (CP) and to L-alanosine (an analogue of aspartate)...
- Products in the T-state of aspartate transcarbamylase: crystal structure of the phosphate and N-carbamyl-L-aspartate ligated enzymeJingwei Huang
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Biochemistry 43:6422-6. 2004..the transition state like those found in our earlier studies of other ligands (N-phosphonacetyl-L-aspartate, carbamyl phosphate plus malonate, phosphonoacetamide plus malonate, or citrate plus phosphate)...
- Weakening of the interface between adjacent catalytic chains promotes domain closure in Escherichia coli aspartate transcarbamoylaseD P Baker
Merkert Chemistry Center, Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02167 3860, USA
Protein Sci 4:258-67. 1995..Solution X-ray scattering was also used to investigate the structural consequences of nucleotide binding to the Glu-50/Asp-100-->Ala enzyme...
- Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi. Insights into cooperative and allosteric mechanismsSigrid Van Boxstael
Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Belgium
FEBS J 272:2670-83. 2005..In contrast to the same enzyme in P. abyssi cellular extracts, the pure enzyme is inhibited by the carbamyl phosphate analogue phosphonacetate; this difference supports the idea that in native cells ATCase interacts with ..
- Structural defects within the carbamate tunnel of carbamoyl phosphate synthetaseJungwook Kim
Department of Chemistry, Texas A and M University, P O Box 30012, College Station, Texas 77843 3012, USA
Biochemistry 41:12575-81. 2002..The partial reactions of CPS have not been significantly impaired by these mutations, and thus, the catalytic machinery at the individual active sites has not been functionally perturbed...
- The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesisV Durbecq
Laboratoire de Microbiologie, Universite Libre de Bruxelles, Brussels, Belgium
Proc Natl Acad Sci U S A 94:12803-8. 1997..The gene coding for this CK-like CPS was called cpkA...
- Thermodynamics of the reactions of carbamoyl phosphateRobert A Alberty
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Arch Biochem Biophys 451:17-22. 2006..As usual the increase in the number of enzyme-catalyzed reactions for which apparent equilibrium constants can be calculated is larger than the number of reactions required to obtain the thermodynamic properties of the species involved...
- Low carbamoyl phosphate pools may drive Lactobacillus plantarum CO2-dependent growth phenotypeFrançoise Bringel
UMR 7156 Université Louis Pasteur, CNRS Génétique Moléculaire, Genomique, Microbiologie, Département Microorganismes, Génomes, Environnement, Strasbourg, France
J Mol Microbiol Biotechnol 14:22-30. 2008..plantarum isolates...
- Protein expression under uracil privation in Lactobacillus sakeiA Marceau
, INRA, Domaine de Vilvert, 78350 Jouy en Josas, France
FEMS Microbiol Lett 200:49-52. 2001..Two of these polypeptides were identified as orotate phosphoribosyl transferase, catalyzing the fifth step of pyrimidine biosynthesis, and PyrR, the transcriptional regulator of the pyr operon, respectively...
- An engineered blockage within the ammonia tunnel of carbamoyl phosphate synthetase prevents the use of glutamine as a substrate but not ammoniaX Huang
Department of Chemistry, Texas A and M University, College Station, Texas 77843, USA
Biochemistry 39:3240-7. 2000..These results suggest the existence of an alternate route to the bicarbonate phosphorylation site when ammonia is provided as an external nitrogen source...
- Glutamine-dependent carbamoyl-phosphate synthetase and other enzyme activities related to the pyrimidine pathway in spleen of Squalus acanthias (spiny dogfish)P M Anderson
Department of Biochemistry, University of Minnesota, Duluth 55812
Biochem J 261:523-9. 1989..Biol. Chem. 262, 198-202] that dogfish express two different glutamine synthetases, and (3) provide indirect evidence that glutamine may not be available in liver for biosynthetic reactions other than urea formation...
- Urea cycle of Fasciola gigantica: purification and characterization of arginaseSaleh A Mohamed
Molecular Biology Department, National Research Centre, Cairo, Egypt
Comp Biochem Physiol B Biochem Mol Biol 142:308-16. 2005..The enzyme was activated with Mn2+, where the other metals Fe2+, Ca2+, Hg2+, Ni2+, Co2+ and Mg2+ had inhibitory effects...
- Altered enzyme activities and citrulline synthesis in liver mitochondria from ornithine carbamoyltransferase-deficient sparse-furash miceN S Cohen
Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033
Biochem J 257:251-7. 1989..In addition, they clarify the role of ornithine transport and of protein-protein interactions in citrulline synthesis in normal mitochondria...
- African sharptooth catfish Clarias gariepinus does not detoxify ammonia to urea or amino acids but actively excretes ammonia during exposure to environmental ammoniaYuen K Ip
Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Road, Singapore 117543, Republic of Singapore
Physiol Biochem Zool 77:242-54. 2004..These results represent the first report on a freshwater fish (C. gariepinus) adopting active excretion of ammonia (probably NH4+) as a major strategy to defend against ammonia toxicity when exposed to environmental ammonia...
- Cloning of cDNA coding for carbamyl phosphate synthetase I and changes in levels of CPS1 mRNA during hepatocarcinogenesisS J Wu
Faculty of Basic Medical Sciences, Peking Union Medical College, Beijing
Sci Sin B 31:197-203. 1988cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insert complementary to the mRNA for CPS1 followed by hybrid-selected translation screening...
- On the origin of biochemistry at an alkaline hydrothermal ventWilliam Martin
Institute of Botany, University of Dusseldorf, 40225 Dusseldorf, Germany
Philos Trans R Soc Lond B Biol Sci 362:1887-925. 2007....
- Y-box binding protein-1 down-regulates expression of carbamoyl phosphate synthetase-I by suppressing CCAAT enhancer-binding protein-alpha function in miceYen Rong Chen
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
Gastroenterology 137:330-40. 2009..Despite expression of C/EBPalpha, CPS1 is not expressed in fetal liver, indicating an additional factor is involved in the regulation of CPS1 expression. The aim of this study was to elucidate the mechanism of CPS1 expression...
- Effects of short-chain acyl-CoA dehydrogenase deficiency on development expression of metabolic enzyme genes in the mouseM E Hinsdale
Department of Comparative Medicine, School of Medicine, University of Alabama at Birmingham 35294, USA
Biochem Mol Med 57:106-15. 1996..SCAD) acyl-CoA dehydrogenases, pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), carbamyl phosphate synthetase I (CPS), ornithine transcarbamylase (OTC), and argininosuccinate synthetase (AS) in fed or fasted ..
- N2-hydroxyguanosine 5'-monophosphate is a time-dependent inhibitor of Escherichia coli guanosine monophosphate synthetaseM L Deras
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
Biochemistry 38:303-10. 1999..In addition, these results complement recent structural studies of GMPS and implicate the formation of the XMP-adenylate intermediate inducing a probable conformational change that stimulates the hydrolysis of glutamine...
- Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCRHeleen Nailis
Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000, Ghent, Belgium
BMC Mol Biol 7:25. 2006..To validate our normalization strategies we determined differences in ALS1 and ALS3 expression levels between C. albicans biofilm cells and their planktonic counterparts...
- Uridine and cytidine nucleotide synthesis in renal hypertrophy: biochemical differences in response to the growth stimulus of diabetes and unilateral nephrectomyS Kunjara
Department of Biochemistry, University College and Middlesex School of Medicine, London, Great Britain
Biochem Med Metab Biol 47:168-80. 1992....
- The dogfish shark (Squalus acanthias) increases both hepatic and extrahepatic ornithine urea cycle enzyme activities for nitrogen conservation after feedingMakiko Kajimura
Department of Biology, McMaster University, Hamilton, Ontario, Canada
Physiol Biochem Zool 79:602-13. 2006..Furthermore, exogenous nitrogen from food is converted into urea not only by the liver but also by the muscle and to a small extent by the intestine...
- Ornithine transcarbamylase deficiency: a urea cycle defectNeil Gordon
Wilmslow, UK
Eur J Paediatr Neurol 7:115-21. 2003..Liver transplant can be considered when symptoms are life-threatening, although there may be severe complications.Gene replacement therapy is the hope of the future...
- Orotic acid excretion and arginine metabolismMargaret E Brosnan
Department of Biochemistry, Memorial University of Newfoundland, St John s, NL, Canada A1B 3X9
J Nutr 137:1656S-1661S. 2007....
- Molecular biology and biochemistry of malarial parasite pyrimidine biosynthetic pathwayJerapan Krungkrai
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Southeast Asian J Trop Med Public Health 34:32-43. 2003..The genes encoding these enzymes were identified in order, from the first to the sixth step, as CPSII (carbamyl phosphate synthase II), ATC (aspartate transcarbamylase), DHO (dihydroorotase), DHOD (dihydroorotate dehydrogenase, ..
- Neonatal pulmonary hypertension--urea-cycle intermediates, nitric oxide production, and carbamoyl-phosphate synthetase functionD L Pearson
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
N Engl J Med 344:1832-8. 2001....
- Effects of assembly and mutations outside the active site on the functional pH dependence of Escherichia coli aspartate transcarbamylaseX Yuan
Department of Biochemistry, University of Minnesota, St Paul 55108, USA
J Biol Chem 271:1285-94. 1996..productive binding of L-Asp to the binary complexes of the catalytic subunit (c3) and holoenzyme (c6r6) with carbamyl phosphate are different...
- [A case of late-onset carbamoyl phosphate synthetase I deficiency, presenting periodic psychotic episodes coinciding with menstrual periods]Y Wakutani
Department of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University
Rinsho Shinkeigaku 41:780-5. 2001..Genomic DNA analysis showed that she was heterozygous in V1149G mutation. The mutation allele was derived from her father. The causative factor for absence or very low level of maternal CPSI mRNA will require further analysis...
- Relationship between carbamoyl-phosphate synthetase genotype and systemic vascular functionMarshall L Summar
Department of Pediatrics, Division of Medical Genetics, Vanderbilt University Medical Center, Nashville, Tenn 37232 6602, USA
Hypertension 43:186-91. 2004..943). These data indicate that a polymorphism in the gene encoding carbamoyl-phosphate synthetase 1 influences nitric oxide production as well as vascular smooth muscle reactivity...
- Expression, purification, crystallization and preliminary X-ray crystallographic studies of a cold-adapted aspartate carbamoyltransferase from Moritella profundaDirk De Vos
Laboratorium voor Eiwitbiochemie en Eiwitengineering, Universiteit Gent, K L Ledeganckstraat 35, Ghent, Belgium
Acta Crystallogr Sect F Struct Biol Cryst Commun 61:279-81. 2005..The three-dimensional structure of the Escherichia coli ATCase was sufficient to solve the structure of the M. profunda ATCase via the molecular-replacement method and to obtain electron density of good quality...
- Differentially expressed genes associated with hepatitis B virus HBx and MHBs protein function in hepatocellular carcinomaDae Ghon Kim
Division of GI and Hepatology, Department of Internal Medicine, Chonbuk National University Medical School and Hospital, Jeonbuk, Republic of Korea
Methods Mol Biol 317:141-55. 2006..secretory trypsin inhibitor (PSTI), H19, guanidine nucleotide-binding protein alpha-1 subunit (GNAZ), carbamyl phosphate synthetase I (CPS I), insulin-like growth factor (IGF)-II, and 10 ribosomal proteins genes...
- Ornithine carbamoyltransferase from the extreme thermophile Thermus thermophilus--analysis of the gene and characterisation of the proteinR Sanchez
Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Brussels, Belgium
Eur J Biochem 248:466-74. 1997..In the presence of its substrates the half-life time was several hours at 85 degrees C. Ionic and hydrophobic interactions contribute to the stability. The expression of T. thermophilus OTC was negatively regulated by arginine...
- Ornithine transcarbamylase and arginase I deficiency are responsible for diminished urea cycle function in the human hepatoblastoma cell line HepG2Demetra Mavri-Damelin
The UCL Institute of Hepatology, Hampstead Campus, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
Int J Biochem Cell Biol 39:555-64. 2007..This strategy could provide a cell source capable of urea synthesis, and hence ammonia detoxificatory function, which would be useful in a bio-artificial liver...
- Lamprey parasitism of sharks and teleosts: high capacity urea excretion in an extant vertebrate relicMichael P Wilkie
Department of Biology, Mount Allison University, Sackville, NB, Canada E4L 1E4
Comp Biochem Physiol A Mol Integr Physiol 138:485-92. 2004....
- Cell cycle-dependent regulation of pyrimidine biosynthesisFrederic D Sigoillot
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
J Biol Chem 278:3403-9. 2003..Thus, the cell cycle-dependent regulation of pyrimidine biosynthesis results from the sequential phosphorylation and dephosphorylation of CAD under the control of two important signaling cascades...
- Integration host factor (IHF) modulates the expression of the pyrimidine-specific promoter of the carAB operons of Escherichia coli K12 and Salmonella typhimurium LT2D Charlier
Research Institute, CERIA COOVI, Brussels, Belgium
Mol Gen Genet 237:273-86. 1993..IHF thus appears to be required for maximal expression as well as for establishment of full repression. IHF could exert this function by modulating the binding of a pyrimidine-specific regulatory molecule...
- Urea cycle enzymes through the development of pacu (Piaractus mesopotamicus): the role of ornithine carbamoyl transferasePaulo Sérgio Monzani
Laboratory of Adaptive Biochemistry, Department of Genetics and Evolution, Federal University of Sao Carlos, Rod Washington Luiz Km 235, CP 676, CEP 13565 905, Sao Carlos, SP, Brazil
Fish Physiol Biochem 34:139-49. 2008..We suppose that OCT during the embryogenesis is a control step regulating the cellular concentration of ornithine for polyamines synthesis...
- Role of the glutamate dehydrogenase reaction in furnishing aspartate nitrogen for urea synthesis: studies in perfused rat liver with 15NItzhak Nissim
Division of Child Development and Rehabilitation Medicine, Department of Pediatrics, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Biochem J 376:179-88. 2003..In addition, the current findings suggest that the formation of aspartate via the mitochondrial aspartate aminotransferase reaction may play an important role in the synthesis of cytosolic argininosuccinate...
- Population screening in a Druze community: the challenge and the rewardTzipora C Falik-Zaccai
Institute of Human Genetics, Western Galilee Hospital Nahariya, Israel
Genet Med 10:903-9. 2008..of metabolism cerebrotendinous xanthomatosis, prolidase deficiency, argininosuccinate lyase deficiency, and carbamyl phosphate synthetase I deficiency were identified in an isolated Druze village in northern Israel...
- Effect of alkalinity (pH 10) on ureogenesis in the air-breathing walking catfish, Clarias batrachusNirmalendu Saha
Biochemical Adaptation Laboratory, Department of Zoology, North Eastern Hill University, Shillong 93 022, India
Comp Biochem Physiol A Mol Integr Physiol 132:353-64. 2002..The activity of glutamine synthetase and three enzymes operating in the urea cycle (carbamyl phosphate synthetase, argininosuccinate synthetase, argininosuccinate lyase) increased significantly in hepatic and ..
- Growth factor-regulated expression of enzymes involved in nucleotide biosynthesis: a novel mechanism of growth factor actionM G Gassmann
Max Planck Institute of Biochemistry, Martinsried, Germany
Oncogene 18:6667-76. 1999....
- Role of ureogenesis in the mud-dwelled Singhi catfish (Heteropneustes fossilis) under condition of water shortageN Saha
Biochemical Adaptation Laboratory, Department of Zoology, North Eastern Hill University, 793022, Shillong, India
Comp Biochem Physiol A Mol Integr Physiol 128:137-46. 2001..The activities (units/g tissue and /mg protein) of glutamine synthetase and three enzymes of the urea cycle, carbamyl phosphate synthetase, argininosuccinate synthetase and argininosuccinate lyase increased significantly in most of the ..
- Organization of carbamoyl-phosphate synthase genes in Neisseria gonorrhoeae includes a large, variable intergenic sequence which is also present in other Neisseria speciesF S Lawson
Department of Biology, University of Ottawa, Ontario, Canada
Microbiology 141:1183-91. 1995..meningitidis and 18 commensal Neisseria isolates comprising nine species. This unexpected organization of the CPS genes in N. gonorrhoeae is therefore widespread throughout the genus Neisseria...
- Structure and catalytic mechanism of a novel N-succinyl-L-ornithine transcarbamylase in arginine biosynthesis of Bacteroides fragilisDashuang Shi
Children s Research Institute, Children s National Medical Center, George Washington University, Washington, D C 20010, USA
J Biol Chem 281:20623-31. 2006..Crystal structures of this novel transcarbamylase complexed with carbamyl phosphate and N-succinyl-L-norvaline, as well as sulfate and N-succinyl-L-norvaline have been determined and refined ..
- A comparison of the effects of environmental ammonia exposure on the Asian freshwater stingray Himantura signifer and the Amazonian freshwater stingray Potamotrygon motoroYuen K Ip
Department of Biological Science, National University of Singapore, Kent Ridge, Singapore 117543, Republic of Singapore
J Exp Biol 206:3625-33. 2003..Our results suggest that such a strategy began to develop in those elasmobranchs, e.g. H. signifer, that migrate into a freshwater environment from the sea but not in those permanently adapted to a freshwater environment...
- Genetic variation in the mitochondrial enzyme carbamyl-phosphate synthetase I predisposes children to increased pulmonary artery pressure following surgical repair of congenital heart defects: a validated genetic association studyJeffrey A Canter
Center for Human Genetics Research, Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN, USA
Mitochondrion 7:204-10. 2007..In conclusion, the CPSI T1405N genotype appears to be an important new factor in predicting susceptibility to increased PAP following surgical repair of congenital cardiac defects in children...
- Effects of crowding on ornithine-urea cycle enzyme mRNA expression and activity in gulf toadfish (Opsanus beta)Tammy Laberge
Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Science, University of Miami, 4600 Rickenbacker Causeway, Miami, FL 33149, USA
J Exp Biol 212:2394-402. 2009..These results suggest that the activities of O-UC enzymes are better predictors for urea production than O-UC enzyme mRNA expression levels...
- Urea synthesis in the African lungfish Protopterus dolloi--hepatic carbamoyl phosphate synthetase III and glutamine synthetase are upregulated by 6 days of aerial exposureShit F Chew
Natural Sciences, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Republic of Singapore
J Exp Biol 206:3615-24. 2003..dolloi possesses transporters that facilitate the excretion of urea in water...
- Converting catabolic ornithine carbamoyltransferase to an anabolic enzymeH Baur
Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Zurich, Switzerland
J Biol Chem 265:14728-31. 1990..coli; the hybrid enzyme had a markedly reduced homotropic cooperativity. This gene fusion method should be generally useful for directed enzyme evolution...
- Structural insight on the control of urea synthesis: identification of the binding site for N-acetyl-L-glutamate, the essential allosteric activator of mitochondrial carbamoyl phosphate synthetaseSatu Pekkala
Centro de Investigacion Principe Felipe, Avda Autopista del Saler 16, Valencia 46012, Spain
Biochem J 424:211-20. 2009....
- The effects of various inhibitors on the regulation of orotic acid excretion in sparse-fur mutant mice (spf/Y) deficient in ornithine transcarbamylaseJ Nelson
Department of Nutrition, University of Montreal, Quebec, Canada
Chem Biol Interact 89:35-47. 1993..The orotic aciduria remained insensitive to acivicin, indicating mitochondria as the source of carbamyl phosphate. However, orotate excretion was significantly decreased by PALA (P < 0...
- Site-directed mutagenesis of ATP binding residues of biotin carboxylase. Insight into the mechanism of catalysisV Sloane
Division of Biochemistry and Molecular Biology, Louisiana State University, Baton Rouge, LA 70803, USA
J Biol Chem 276:24991-6. 2001..Included in this superfamily are d-Ala d-Ala ligase, glutathione synthetase, carbamyl phosphate synthetase, N(5)-carboxyaminoimidazole ribonucleotide synthetase, and glycinamide ribonucleotide ..
- The use of nucleotide analogs to evaluate the mechanism of the heterotropic response of Escherichia coli aspartate transcarbamoylaseJ B Sakash
Department of Chemistry, Merkert Chemistry Center, Boston College, Massachusetts 02467, USA
Protein Sci 9:53-63. 2000....
- 3-isobutylmethylxanthine inhibits hepatic urea synthesis: protection by agmatineItzhak Nissim
Children s Hospital of Philadelphia, Division of Child Development, Department of Pediatrics, University of Pennsylvania School of Medicine, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
J Biol Chem 283:15063-71. 2008..Together, the data may suggest a new experimental application of IBMX in studies of CPS-I malfunction and the use of agmatine as intervention therapy...
- Substrate binding and catalysis in carbamate kinase ascertained by crystallographic and site-directed mutagenesis studies: movements and significance of a unique globular subdomain of this key enzyme for fermentative ATP production in bacteriaSantiago Ramon-Maiques
Instituto de Biomedicina de Valencia Consejo Superior de Investigaciones Científicas and Centro de Investigación Biomédica en Red de Enfermedades Raras Instituto de Salud Carlos III, Jaime Roig 11, Valencia 46010, Spain
J Mol Biol 397:1261-75. 2010..It is concluded that the PSD movement allows CK to select against the abundant CP/carbamate analogues acetylphosphate/acetate and bicarbonate, rendering CK highly selective for CP/carbamate...
- Genomic organization of the human gene (CA5) and pseudogene for mitochondrial carbonic anhydrase V and their localization to chromosomes 16q and 16pY Nagao
Edward A Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, Missouri 63104, USA
Genomics 28:477-84. 1995..FISH analysis allowed CA5 to be assigned to chromosome 16q24.3. An unprocessed pseudogene containing sequence homologous to exons 3-7 and introns 3-6 was also isolated and was assigned by FISH analysis to chromosome 16p11.2-p12...
- A single mutation in the active site swaps the substrate specificity of N-acetyl-L-ornithine transcarbamylase and N-succinyl-L-ornithine transcarbamylaseDashuang Shi
Children s National Medical Center, The George Washington University, Washington, DC 20010, USA
Protein Sci 16:1689-99. 2007Transcarbamylases catalyze the transfer of the carbamyl group from carbamyl phosphate (CP) to an amino group of a second substrate such as aspartate, ornithine, or putrescine...
- Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssiYing Xu
J M Wiame Research Institute, Microbiology, Free University of Brussels, B 1070 Brussels
J Bacteriol 185:2161-8. 2003..OTCase(Mab) adaptation to cold indicates a trade-off between affinity and catalytic velocity, suggesting that optimization of key metabolic enzymes at low temperatures may be constrained by natural limits...
- Shifting patterns of nitrogen excretion and amino acid catabolism capacity during the life cycle of the sea lamprey (Petromyzon marinus)Michael P Wilkie
Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, N2L 3C5, Canada
Physiol Biochem Zool 79:885-98. 2006..The subsequent generation of energy-rich carbon skeletons can then be oxidized or retained for glycogen and fatty acid synthesis, which are essential fuels for the upstream migratory and spawning phases of the sea lamprey's life cycle...
- Adenovirus preterminal protein binds to the CAD enzyme at active sites of viral DNA replication on the nuclear matrixP C Angeletti
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham, 35294 0005, USA
J Virol 72:2896-904. 1998..Amino acid sequencing of the 150-kDa band revealed sequence identity to carbamyl phosphate synthetase I (CPS I) and a high degree of homology to the related trifunctional enzyme known as CAD (for ..
- Nitrogen metabolism in the African lungfish (Protopterus dolloi) aestivating in a mucus cocoon on landShit F Chew
Natural Sciences, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Republic of Singapore
J Exp Biol 207:777-86. 2004..Our results indicate that a reduction in ammonia production and decreases in hepatic arginine and cranial tryptophan contents are important facets of aestivation in P. dolloi...
- The amidotransferase family of enzymes: molecular machines for the production and delivery of ammoniaF M Raushel
Department of Chemistry, Texas A and M University, College Station 77843, USA
Biochemistry 38:7891-9. 1999..Molecular tunnels between distant active sites have also been identified in tryptophan synthase and glutamine phosphoribosyl pyrophosphate amidotransferase and are likely architectural features in an expanding list of enzymes...
- Opposite effects on regulation of urea synthesis by early and late uraemia in ratsSusanne Schouw Nielsen
Department of Medicine V Hepatology and Gastroenterology, Aarhus University Hospital, 44 Noerrebrogade, DK 8000 Aarhus C, Denmark
Clin Nutr 26:245-51. 2007..The aims were to investigate the effects of early and late experimental renal failure on the regulation of hepatic urea synthesis and the expression of urea cycle enzyme genes in the liver...
Research Grants76
- Genotype Analysis for Diagnosis of Urea Cycle DisordersSteven Dobrowolski; Fiscal Year: 2007..Products of the following genes are required for ureagenesis: N-acetylglutamate synthetase carbamyl phosphate synthetase 1 , ornithine transcarbamylase , argininosuccinate synthetase , argininosuccinate lyase , ..
- REGULATION OF 15N UREA ISOTOPOMERS PRODUCTIONItzhak Nissim; Fiscal Year: 2010..Our preliminary results suggest that such treatment is feasible. The outcome of the propose study may provide new guidelines for evaluation, diagnosis and treatment of defective urea synthesis in NAFLD or MS. ..
- REGULATION OF 15N UREA ISOTOPOMERS PRODUCTIONItzhak Nissim; Fiscal Year: 2010..Our preliminary results suggest that such treatment is feasible. The outcome of the propose study may provide new guidelines for evaluation, diagnosis and treatment of defective urea synthesis in NAFLD or MS. ..
- STRUCTURE AND FUNCTION OF CARBAMYL PHOSPHATE SYNTHETASECAROL LUSTY; Fiscal Year: 1993The synthesis of carbamyl phosphate, an intermediate in pyrimidine and arginine biosynthesis, is catalyzed by ammonia and glutamine dependent carbamyl phosphate synthetase (CPS)...
- MECHANISMS OF THYROID HORMONE ACTION IN DEVELOPMENTVALERIE GALTON; Fiscal Year: 1991..The levels of translatable mRNA for carbamyl phosphate synthetase (CPS) present in liver during spontaneous and induced metamorphosis will be determined and ..
- COMMON POLYMORPHISM EFFECTS ON UREA CYCLE FUNCTIONMarshall Summar; Fiscal Year: 2001..My research has focused on characterizing the gene encoding the first, rate-limiting step of ureagenesis, carbamyl phosphate synthetase I (CPSI)...
- Nitric Oxide Precursors and Congenital Heart SurgeryFrederick Barr; Fiscal Year: 2007..Finally, a genetic single nucleotide polymorphism (SNP) in the rate limiting urea cycle enzyme, carbamyl phosphate synthetase I (CPSl T1405N), appeared to affect postoperative plasma arginine levels and pulmonary vascular ..
- N-carbamylglutamate in the treatment of hyperammonemiaMendel Tuchman; Fiscal Year: 2009..that cause hyperammonemia and consequent brain damage: N-acetylglutamate synthase (NAGS) deficiency, carbamyl phosphate synthetase I (CPSI) deficiency, propionic acidemia (PA), methylmalonic acidemia (MMA) and hyperinsulinism ..
- REGULATION OF 15N UREA ISOTOPOMERS PRODUCTIONItzhak Nissim; Fiscal Year: 2007..The proposed studies are of clinical as well as scientific significance. The data to be generated may have tremendous clinical impact by advancing the development of a protocol to ameliorate impaired urea synthesis. ..
- REGULATION OF 15N UREA ISOTOPOMERS PRODUCTIONItzhak Nissim; Fiscal Year: 2002..A long term goal is to apply our experimental approach to human subjects in cases such as sepsis, hepatic encephalopathy and/or perturbed hormonal status, such as in diabetes. ..
- GENE STRUCTURE OF CARBAMYL PHOSPHATE SYNTHETASECAROL LUSTY; Fiscal Year: 1992b>Carbamyl phosphate synthetase is an important housekeeping enzyme that provides carbamyl phosphate for arginine and pyrimidine biosynthesis. Our previous studies on the carbamyl phosphate synthetase genes of E...
- Structural Studies of the Pyrimidine PathwayJacqueline Vitali; Fiscal Year: 2004..Finally, understanding the means of extreme heat stability will allow us to engineer selected features in other aspartate transcarbamoylases to obtain enzymes with enhanced thermostability. ..
- CONTROL OF PYRIMIDINE BIOSYNTHESIS IN MAMMALIAN CELLSDavid Evans; Fiscal Year: 1993..The first three enzymes of the pathway, carbamyl phosphate synthetase, aspartate transcarbamylase and dihydroorotase are carried by a single 230 Kd polypeptide chain...
- T. Gondii: Pyrimidine Synthesis as a Chemo TargetDavid J Bzik; Fiscal Year: 2010..Consequently, this research project will accelerate the discovery of new vaccines and treatments for devastating opportunistic parasite infections in AIDS. ..
- Structure-Function Analysis of EnzymesHazel Holden; Fiscal Year: 2004....


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The Good Shepherd Hospital chapter of Breast Cancer Network of Strength meets on the third Thursday of every month for those who need help through a rough spot in their lives. Breast Cancer Network of Strength trained facilitators lead each meeting, offering support as well as education to breast cancer patients. Facilitators are patients themselves, and they understand the questions and concerns that arise with a breast cancer diagnosis.
Backed by hospital staff and physicians when they need additional resources, Breast Cancer Network of Strength facilitators help group members address and resolve the physical and emotional stresses cancer patients may face.
Breast Cancer Network of StrengthTM (formerly Y-ME) is a Chicago-based national nonprofit organization with the mission to ensure, through information, empowerment and peer support, that no one faces breast cancer alone.
At the center of the organization is the National Breast Cancer Network of Strength Hotline, the only 24/7call center operated by trained peer counselors who are breast cancer survivors. Hotline calls are interpreted in 150 languages.


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U.S. Supreme Court
Ginsburg to officiate at same-sex marriage ceremony
Posted Aug 30, 2013 5:38 PM CDT
By Molly McDonough
Justice Ruth Bader Ginsburg will conduct a same-sex marriage ceremony Saturday when Ginsburg's close friend, Kennedy Center President Michael M. Kaiser, marries economist John Roberts. She'll be the first U.S. Supreme Court justice to officiate a same-sex ceremony.
The Washington Post notes that the gala wedding at the Kennedy Center "brings together the nation's highest court and the capital's high society, and will mark a new milepost in recognition of same-sex unions."
Unusual just a decade ago, same-sex marriages are now legal in Washington, D.C.; in 13 states; and all or part of 17 countries, the Post notes.
More legal challenges are mounting in the United States following the June Supreme Court ruling which struck down the Defense of Marriage Act that banned federal benefits to same-sex married couples.
The Post described Ginsburg as excited to perform the ceremony, and noted she has another one planned in September.
"I think it will be one more statement that people who love each other and want to live together should be able to enjoy the blessings and the strife in the marriage relationship," Ginsburg is quoted saying.
There's a long tradition of Supreme Court justices officiating at weddings, but mostly of former law clerks, close friends or relatives. The Post notes that Ginsburg officiated at her son's wedding and that Justice Clarence Thomas performed a ceremony for Rush Limbaugh.
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Company says "thoughts are with family" of worker crushed to death by forklift truck
The employer of a man killed when he became trapped under a forklift truck has released a statement saying its thoughts are with his family.
The man was working at Severfield-Watson Structures near Thirsk when he was crushed by the vehicle. The Health and Safety Executive is investigating.
– Spokesman, Severfield-Watson
"We can confirm that on Wednesday 13 March an incident at our Severfield-Watson Structures production plant in Dalton led to the tragic death of a company employee.
At this moment, our thoughts are with the employee's family. A full investigation is underway and until this is completed the Company is not in a position to comment any further."


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Police are still trying to catch a suspect who shot a man dead while he was changing a flat tire on his car along Interstate 95 near Brunswick.
Glynn County police say Richard Hollis, 49, was found shot to death next to his car on the side of the road.
Witnesses reported seeing a small red or maroon car speeding off on I-95. Police aren't speculating on a motive, but they say Hollis' wallet was missing.
Hollis was traveling north from Jacksonville to North Carolina to visit relatives when he got a flat tire. He pulled over on the side of the road to change the tire near the Hwy 99 interchange.
"Obviously, some closure would be nice. I think the detective and the people on the case have been pretty generous and awesome dealing with that and communicating with the family and stuff," said Blake Dunbar, a family friend.
Anyone with information on the shooting is asked to call the Glynn County Police Dept.
Copyright 2012 WTOC. All rights reserved.
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Savannah, GA 31412
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By Richard Wright
Friday, February 7, 2014
BLack Prince tomatoes from D. T. Brown.
GARDENING GRAFTING is, of course, an age-old technique, which combines the best attributes of a rootstock with that of the stem joined to it.
It is a marriage of two plants that are good at what they do — so producing an amalgam that is good at everything. That's the theory.
Fruit trees have been subject to this since ancient times and it is therefore surprising the technique did not cross over to other produce until the 1920s when American, Korean and Japanese growers experimented to reduce fungal infections, which were destroying watermelon crops.
The Dutch horticulture industry began grafting cucumbers soon after the war but it was not until 1962 the first commercially grown grafted tomatoes started to appear in Europe.
Now they dominate the industry.
But it was a surprisingly slow rate of progress that was mirrored by how long it took for grafted plants to cross over from horticultural production to the home grower.
For instance, it was not until 2011 I first came across grafted chillis at the Hampton Court Flower Show, noticing them not only because of the profusion of peppers but for the fact that the Sutton's variety bore the name Medina.
The scion had been grafted onto vigorous rootstock.
Then I came across what I called the totato.
Both members of the same family, potatoes and tomatoes can be grafted together to get potatoes and tomatoes from the same plant.
It never used to be a hugely happy or productive union but it is a bit different — and if you want to have a go, there is a YouTube instruction video link on the Dobies of Devon website.
For those who want ready-made novelty, Thompson & Morgan has taken the plunge and produced TomTato, now available to the home grower.
Even if the point of paying £14.99 for a single plant, albeit including a supply of chemical fertiliser, may be lost on me, I can see its novelty.
T&M pointed to there being a potential harvest of more than 500 cherry tomatoes above ground with a Brix (sweetness) level way above that of supermarket tomatoes — and good acidity too.
But, below ground, T&M said there would also be up to 2kg of versatile white potatoes.
They can be grown in a greenhouse, in a sunny garden spot or large patio pot.
There's also a TomTato video on the T&M website.
Dobies and several of the other seed companies now have large ranges of grafted plants but, as is usual in this life, there are no positives without negatives.
While the positive is increased yield, vigour (to such an extent that two leading stems can be trained) and disease resistance, the negative is cost.
For instance, the F1 Lizzano outdoor tomato caught my eye but just a single plant costs £3.99 — more expensive than a packet of the most costly F1 seed.
Three 'plugs' cost £9.99, which sounds a lot — until you extrapolate just how much the fruit produced would cost you at the greengrocer.
Lizzano is of special value because it is very resistant to late blight, which tends to bring cropping to a premature end in this country as the nights become chill and the days damp — a perfect breeding ground.
Yet another plus point of grafted tomatoes is that because greenhouse varieties are also extremely disease resistant they can be planted directly in the soil — which is always best for taste — and you don't have to be too picky about total soil replacement season after season.
A very eye-catching Dobies indoor variety is Sweet Petit.
It is a vigorous-growing cherry tomato, producing long, fishbone-shaped trusses of very attractive, shiny, red fruit.
They are described as 'deliciously sweet and tasty fruit. Ideal for heated and unheated glasshouses or polytunnels'.
For those who want a tomato-potato combo for £14.98, there is an offer of three customers' favourite grafted tomato plants and six tubers each of customers' favourite potatoes.
Grafted turbo-charged fruit has now been rolled out by the seed houses to include cucumbers, peppers, aubergines, squashes and melons.
The reason for the limit to only certain fruits? It is labour-intensive, therefore expensive, and can only realistically be applied to higher-value crops.
I must admit this is the first year I am considering trying a couple of grafted varieties but that comes after reading considerable comparison studies.
One such was in Grow It! magazine where the reviewer concluded the growth, yield and length of the fruiting season made it a worthwhile investment.
"The trial worked better than I could have imagined," she concluded.
"Grafted tomatoes are, without a doubt, worth every penny of the initial cost.
"The expense is balanced out by the reliability of the plants and the total yield of fruits taken. For speed of growth, disease resistance, reliability, crop size and flavour, grafted versions outperformed any other variety I have ever grown.
"The only word of caution would be this: make sure you have plenty of very long bamboo canes or other supports at hand. Turbo-charged tomatoes live up to their name! "
One that caught my eye at D. T. Brown was Black Prince.
This was a Russian variety I tried a few years ago now.
Very juicy and sweet, I was disappointed by its lack of disease resistance, so a grafted version should sort that out with a bit of luck.
Brown's describe it as: "Very distinctive with dark purple to black coloured fruits and a delicious, rich and fruity flavour, this wonderful variety originates from Siberia and is known to perform well in cooler climates."
But one of its greatest attributes is it looks absolutely beautiful on the plate.
Also from D. T. Brown is the Supersweet F1, small cherry tomatoes which have a very sweet taste. This prolific fruiter was in the top three of the 2007 Tastiest Tomato competition.
For those who like a beefsteak variety, there is the distinctive Coeur de Boeuf, which has an element of Northwood grower Stan Jackson's Queen of Hearts about it. It has Stan's heart-shape but it is also interestingly ribbed.
Brown's said it has mouth-watering texture and flavour.
"Very meaty and fleshy, fruits ripen from the inside out and look particularly attractive when sliced and used in Mediterranean type salads with mozzarella and olive oil."
That's got me really salivating for a taste of summer.
• For more details, see www.dobies.co.uk, www.dtbrownseeds.co.uk and www.thompson-morgan.com
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News: "If There's Any Woman In My Life That's The Ideal Woman For Me, It's Definitely Nicki [Minaj]"
Sunday, Nov 20, 2011 11:00AM
Young Money star Drake is no longer holding back his love for labelmate Nicki Minaj and recently described her as the perfect woman for him.
In Drizzy's opinion, the self-proclaimed Barbie stands out from other ladies because she fully understands him.
"If there's any woman in my life that's the ideal woman for me, it's definitely Nicki," said Drake. "I like the stripped-down Nicki. I like Nicki with no makeup, black hair, some casual clothes in a recording booth rapping an amazing verse. That's sexy to me." He added, "I know some great women, but all jokes aside, Nicki is somebody I could spend my life with because I think we understand each other." (The Daily Beast)
In October, Drake said he still had high hopes of marriage to Nicki.
I don't know if we were really pretending. I'd marry Nicki. I think Nicki would be one of the only people that would understand me at the end of all of this and be able to love me," Drake reveals about his Young Money group member. "I guess I thought it was more [with me and Rihanna]. That was the first girl with any fame that paid me any mind." (Elle Magazine)
Last month, Nicki entertained the idea of starting a family in the future.
Having gotten past those low points, the MC tells the magazine that she has firm plans for a bright future. "I will have put out five albums and will have an Oscar and Grammys," she predicted. "And I will be getting married and, a couple of years after that will have a bun in the oven. ... My whole life is about being NickiMinaj now. It's a never-ending saga." (MTV)
After fooling fans claiming they married in summer 2010, Nicki denied reports suggesting she really became Drizzy's wife.
"No, [DJ] Drama, don't be silly," Minaj told DJ Drama when asked about expecting kids with Drake. "Drake is just my little brother and I kid with him all the time. That's all. Yes, yes, we were just being crazy and I think we were a little bit bored. I wouldn't say we were bored, but we were being just mischievous. I did another interview and they asked me and I said, everyday I see things written about me that aren't true. So I wanted to kinda give the blogs a little bit of payback and put my own story out. [laughs] I don't date boys, I don't like boys. [laughs] It's true -- I think boys are yucky and that's it. There's nothing more to say." (Hot 107.9)
Check out some recent Drake and Nicki Minaj footage below:


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Is this okay?
Is this okay?
I am a Christian, and not ashamed to admit it! Copy and paste this if you are too.
"For God so loveth the world, that he hath given his only son, that none that believe in him, should perish: but should have everlasting life." (John 3:16)
My Animelist
Sig check please, also I got permission to use the Chespin sprite from the maker on deviantart, put who made it in sig.
Back home! TO HOENN!
Poochyena is claimed by me!
Spoiler:- Who made what:
Flame sprite by Ashley on Pokecharms. New mug and walking sprite by me.
Is mine okay? It's 600x300 pixels.
Sig check please~ i have a feeling it's too tall... <.>;
Siggy check. My comp tells me the gif is 245 x 170 with 410 KBs.
I have claimed Alucard (Hellsing), Sebastian (Kuroshitsuji), and Laguna Loire (Final Fantasy VIII).
Hellsing, Street Fighter, The Elder Scrolls, Dangan Ronpa, Bleach, Homestuck, Black Butler, and Puella Magi Madoka Magica fan.
Sig made by Izagar.
3DS FC: 3738 0398 8876 Mikan
Friend Safari: Ground (Wooper, Camerupt, and Palpitoad)
I needz a wittle checky-poo please.
Credit to FrozenConstellation
Signature check please~
Art Shop ~ Tumblr ~ Personality Test ~ Photobucket ~ DeviantArt
I've redesigned my signature completely again, could you check it? Please and thank you!
Ash: Now I choose you!
Electro Ball!
-Pikachu: PikaPika-Chu-Pi!-
I think my signature is too tall, at least, a Moderator hinted at it, I guess. Check, please?
Mine is an unchanging love, higher than the heights above, deeper than the depths beneath, free and faithful, strong as death.
William Cowper, "Lovest Thou Me?" Olney Hymns
I choose my words carefully, but they may still hurt someone accidentally...
Marley, Pokemon Diamond and Pearl
And, look you, there is nothing in this thing of learning out of books. Here, here and here (pointing to his ear, his head and his heart) is your school. If everything is right there, then take your pen and down with it; afterward ask the opinion of a man who knows his business.
Wolfgang Amadeus Mozart
Images removed for making the signature too tall.
I think the gif made it too big,check please?
Check please?
Sig check please!
Demon Kitsune Yoko Kurama Safe
Monster Guy File size too large
pokemonsquared Member banned - no signature shown
Elysian Too tall
RocketToTheMoon File size too large -Anime Gif
joej m File size too large -Club Double Trouble Leader Banner
MuseHarmonia Safe
SpyroxPikachu Image size Too large
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Shooting Quazard Dragon Image safe for signatures.
EternalSword7 Safe
Cobali Image size Too large
deoxysdude94 Safe
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Flame Haji Safe
Eeveelover824 Safe
Gothic-Gothorita Safe
βƹβƹ ɱɪɢηѻη Image size too large
brother Image size too large
FlareLover17 Safe
Locked In My Asylum Safe
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Shiny_Wooper File size too large -N's Gif
Azran Flame Safe
Pilpulp Safe
Writing LJ Twitter Raptr
MikeSharpeWriter.com -Project Elfish Jarate
3DS - 3007 - 8991 - 4413 (VM/PM me to add you)
The image is resized, is it good now?
Credit to FrozenConstellation
Sig check please
The marriage between Princess Peach from the Mario world and Cractus from the Wario world will begin
3DS FC: 2552-0845-5282Feel free to add me ^_^
Check please?
Sig check, please?
Alright, the image has been resized, how are we now?
I removed the large file. Is it better?
Sig Check please
Thank you.
✿If I have any worth, it is to live my LIFE for GOD✿
|| Multishipper || Red: ShinAya= Kagepro OTP || Inactive ||


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By Kontji Anthony - bio | email | Facebook
MEMPHIS, TN (WMC-TV) - Rainbow PUSH Coalition president LaSimba Gray hosted a handful of critical leaders at Monumental Baptist Church Saturday to share opposition to the March 8 referendum to transfer Memphis schools to Shelby County."Resolved to defeat this referendum," said Gray. "There's no benefit to consolidation."The Memphis Teachers Association, AFSCME, Action National Network, and SCLC heard from former Chattanooga Schools Board commissioner Sherman Matthews, who urged them to keep schools separate."It does not save any money," said Matthews. "It's not going to provide any better education for any child."Tennessee Representative G.A. Hardaway was the lone referendum supporter at the forum."I understand that they have a problem, but it does not apply to us," said Hardaway. "If there's anything that we can do to help, we'd be happy to come to Chattanooga and give them advice."A passionate Memphis Schools Board Commissioner Sara Lewis said poverty must first be addressed."Someone needs to gut up, and man or woman up and do that," said Lewis.Representative Hardaway then fired a series of questions at Shelby County Schools Chairman David Pickler."Mr. Pickler, even though I like him, tends to twist and distort the facts," he said."There are passionate feelings on both sides," said Pickler.The two went toe-to-toe over Pickler's claims that Memphis City Schools will lose money in a merger."The children of Memphis City Schools stand to lose a quarter of a billion dollars in funding and resources," said Pickler.Hardaway denied Pickler's claim."We have facts out there," said Hardaway. "And then we have facts as presented by those who oppose what is going to happen."Hardaway said the city could cut $780 per student at most. He said city leaders assured him they would keep funding in place.
Copyright 2011 WMC-TV. All rights reserved.
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Commonweal Retreat Center
Commonweal is a nonprofit center for service and research in health and human ecology located on a beautiful 60-acre site at the edge of the Pacific Ocean in the Point Reyes National Seashore. Our three guest houses are available, when not in use for Commonweal programs, to small groups and individuals for conferences, workshops, meditation and yoga retreats, family gatherings, as well as individual retreats, and similar uses which are compatible with Commonweal's nonprofit purpose.
Pacific House
|Pacific House, beautiful and historic, sits on a bluff overlooking the Pacific. The house is comfortably furnished and electrically heated throughout.
Bothin House
|Bothin House is situated in a secluded grove of Monterey pine and cypress trees.
Kohler House
|Kohler House sits in an open setting with partial views of the ocean.
Guests at Commonweal should prepare for dramatic changes of weather at almost any time of year. The winter may bring frequent rain, and this coastal site is often windy. We suggest warm, layered clothing and good walking shoes so you can enjoy the extraordinary resources of the Point Reyes National Seashore.
This is a place for people who enjoy the isolation and accept the occasional inconveniences of a rural retreat center.
- Commonweal is situated on 60 acres in the Point Reyes National Seashore
- Beach access
- Sauna is available for guests
- Walking trails
Photos by Philip Buchanan
The Retreat Center at Commonweal Requires a Two-Night Minimum Stay at All Three Houses
- Weekends consist of Friday and Saturday nights or Saturday and Sunday nights; a week consists of 6 nights.
- A NON-REFUNDABLE deposit is required at the time the reservation is made, in the amount of the single-night rate for the number in your group, or $500 if you do not know the number in your group.
- Your reservation is not confirmed until your deposit is received. If we do not receive your deposit check within 10 working days, we will not be able to hold your reservation.
- Visa, Mastercard, business or personal checks are accepted.
- Check-out time is 12:00 noon. Check-in time is 3:00 p.m., unless other arrangements are made.
- If a late check-out is requested, it may be extended until not later than 5:00 p.m., if it does not conflict with the cleaning schedule.
- All Commonweal guest houses are available for day-long retreats during the week.
For additional information and reservation schedules, please email or call Jenepher Stowell, Retreat Center Director, 415-868-0970 (voice mail extension 315).
2011 – 2014 Rates:
Pacific House: Two night minimum – up to 12 people
|Two nights
|Three nights
|Four nights
|Five nights
|One week
Bothin House: Two night minimum – up to 4 people
|Two nights
|Three nights
|Four nights
|Five nights
|One week
Kohler House: Two night minimum – up to 6 people
|Two nights
|Three nights
|Four nights
|Five nights
|One week
We regret that, despite our love of animals, pets are not allowed at the Retreat Center.
Directions to Commonweal
Commonweal is located in Bolinas, California at
451 Mesa Road, Bolinas, CA 94924.
- Download a map with driving directions from San Francisco, East Bay, and San Rafael
- Click here for driving directions from Santa Rosa, Petaluma, and Vallejo


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Town Square
Young art students win scholarships
Original post made on Apr 14, 2012
Read the full story here Web Link posted Friday, April 13, 2012, 10:00 AM
on Apr 14, 2012 at 12:35 pm
I've paid out-of-pocket nearly $7,000 on CSMA classes. People like me should be thanked as well.
on Apr 17, 2012 at 2:19 pm
And, thanks to the Mountain View Educational Foundation, who is the organization who pays for CSMA to come to the elementary schools.
Not only would these students not have gotten scholarships, but they would probably not have had art/music at all if not for generous donations from parents and local businesses to MVEF.
on Apr 17, 2012 at 9:06 pm
Congratulations to the students who received scholarships!
We are extremely lucky to have the Mountain View Educational Foundation. If it weren't for them, we probably wouldn't have art and music classes at all in the district. Do drop in on their web site (Web Link) read up on what they do, and consider donating a little something.


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It's another busy on-ice week for the NHL Network, which will show games on three consecutive nights, featuring two of the League's hottest scorers and one of its most intense rivalries.
On Thursday night, the NHL Network will show the game between the Tampa Bay Lightning and Philadelphia Flyers from the Wells Fargo Center. The Flyers have been one of the NHL's hottest teams, but they'll have to shut down Tampa Bay's Steven Stamkos, the NHL's leading scorer.
The action moves to Atlanta on Friday, where the Thrashers will try to slow Alex Ovechkin and the rest of the Washington Capitals. It's the Caps' first visit since opening night, when Atlanta skated off with a 4-2 win.
One night later, hockey fans in the United States can tune into the NHL Network for a Hockey Night in Canada telecast from Montreal, with the Canadiens hosting the Toronto Maple Leafs. It's the Leafs' first visit to the Bell Centre this season, and the first meeting between the teams since Toronto's 3-2 win on opening night.


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Videos > Lucky Benton > Sexy hot milf Lucky Benton getting so screwed in her snatch she cant wait to cum
Soccer Mom Score There's no double that the MILFs on Soccer Mom Score have some of the hottest mature muffs in the business! They have the experience and the bodies to milk your rod of all its worth - check it out.
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Working Group for Early Childhood Learning Meets for 2nd TimeBy Lawayne Childrey | Published 08 Dec 2010 08:25am |
Mississippi remains the only state in the south that does not have a state funded Pre-K program. MPB's Lawayne Childrey reports on what experts from around the country told the Lt. Gov's working group on Early Childhood Learning, about the pros and cons of Mississippi developing it's on pre-k system.
Lawmakers on both sides of the aisle agree that education is the key to the future of Mississippi. But before they commit to any one plan or plans they are soliciting the expertise of professionals in the field like Dr. Steve Suitts, Vice President of the Southern Education Foundation.
"This is a problem because kids aren't ready to go to school and what preschool does in a play environment is teach kids how to be ready."
But, Forest Thigpen, President of the Mississippi Center for Public Policy asks... is it justifiable to have the state invest billions of dollars over time in a system that we don't really know improves childhood education in the long term?
"There are schools that have taken kids at 5th grade who are scoring at the 40th percentile compared to their peers and taken them to the 80th percentile in just four years. There are ways to accomplish success without creating a brand new very expensive early education program."
Lieutenant Governor Phil Bryant supports level funding for K-12 Education, but hopes to encourage more choice, competition and accountability in public schools, to accomplish what a pre-school system would bring..
"If these children are not prepared to go to school, if they dropout in the 9th grade we're going to lose the workforce that we desperately need in the next decade."
Suitts says Mississippi has a big problem because it has a larger number of students who have to repeat kindergarten and first grade than any other grade in the elementary system. Lawayne Childrey MPB News.
CommentsMPB will not tolerate obscenities, threats/personal attacks, hate speech, material that is ethnically or racially offensive, abusive comments, comments off topic and spam, to name a few. You can see a complete list of the MPB guidelines by viewing our terms of service. If you spot a comment you think violates these guidelines, report it to the moderators by clicking "x" next to the comment, then "report". MPB reserves the right to adjust these guidelines. If you have a suggestion, please contact us.


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Department of Sociology at Purdue University has developed one of the nation's premier programs in the sociology of religion. The program pursues excellence and national prominence in three areas: discovery, learning, and engagement. We welcome you come to study Sociology of Religion at Purdue University. http://www.cla.purdue.edu/sociology/programs/sociologyofreligion/
B.A. in Sociology, China University of Political Science and Law, China
M.A. in Sociology, Peking University, China
Research Interests: Politics and Religion; Political Sociology
B.A. in English, Air Force Command Academy, Beijing, China
M.A. in Political Science, Renmin University of China, Beijing, China
Research Interest: Politics and Religion; Faith-based Social Movements; The Chinese Protestant House Church Movement
B.A. in English Language and Literature, Peking University, China
M.A. in English Language and Literature, Peking University, China
M.A. in Sociology, Central Michigan University.
Research Interests: Religion in China; Comparative Studies of American and Chinese Religions; Sociological Theory
Lecturer, Department of Sociology, School of Social Development and Public Policy, Fudan University
Ph. D. in Sociology, Purdue University
Dissertation: Folk Religion in Chinese Socieites
M.A. in Sociology, Fudan University, Shanghai, China
B.A. in Sociology, Fudan University, Shanghai, China
Research Interests: Sociology of Religion; Research Methods; China Study