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The NFL finally got it right.
Hold on there ladies and gents, put down the pitchforks. Mechem, please don't put me in your hate guide. I'm not talking about the decision to call yet another phantom "blow to the head" on a Steelers defender. I'm speaking on the NFL's decision to not fine Ryan Clark for what some are calling a flagrant hit, despite the lack of any contact between Ryan Clark and Victor Cruz's head.
Somewhere Joe Greene, Deacon Jones, Jack Lambert, and Dick Butkus sit at the bar of incomparable manhood and snort at the state of NFL.
According to this drivel from Shutdown Corner, one reporter believes Clark should have heard from the NFL for his latest big hit on poor, "unsuspecting" wide receiver. Forgive me as I step out to vomit. Here's the paragraph in question so that you don't have to waste anymore of your life than necessary following that link:
"But it's also very clear that Clark was looking to inflict pain on a receiver that wasn't in position to defend himself. He honed in on Cruz in the end zone, saw an opportunity to strike when Cruz wasn't looking, and hammered him. It's hard to watch Clark through the play and not come to the conclusion that his only intention was to hit a vulnerable receiver who realistically had no chance to catch the ball after Ike Taylor batted it away and had no idea Clark was coming. The hit was, at the very least, unnecessary."
Now I don't know Mr. Greene, Mr. Jones, Mr. Lambert, nor Mr. Butkus, but if I had to guess, their reaction to this demonizing of "looking to inflict pain" on a football field would have to range between incredulous to disgust. Football has always been a violent game, and while the NFL should do its best to protect its players in regards to head injuries, they might as well make the conversion to flag/touch football if Clark's hit is deemed "unnecessary."
Let's take a look.
Link: The definitive angle (All stills taken from that video in the link):
A blurry first shot, but this is the moment of first contact between Taylor and the ball. Cruz has his hands raised in anticipation of the pass. Clark, seeing this, locks in on the wide receiver.
A much clearer image now shows Clark on a bee-line for Cruz. His hips, chest and head are all directly aligned with Cruz. At this point his peripheral vision does not fully capture Ike Taylor or the ball. He only sees Cruz with his hands up, ready to receive a pass.
A step closer and it's clear-see both feet planted in the turf, ready to spring, and left elbow and forearm rise- Clark is committed to delivering a hit. Cruz's hands are still up, still not ruling out the threat of an incoming pass.
So there can be no doubt, Clark obviously leads with the shoulder as he and Cruz collide. Cruz's hands are finally lowered, partly due to the tremendous force of the hit
Finally, the tape confirms what we all already knew; at no point did Clark hit Cruz in the head. The ball has yet to hit the ground, and thus no whistle has blown yet.
Going back to Frank Schwab, the writer of the aforementioned article, who argues that Clark's "only intention was to hit a vulnerable receiver who realistically had no chance to catch the ball." This is false. As depicted by the stills, it's clear Victor Cruz held his hands in a position that would suggest the ball was still on its way. For Clark, the only logical thing to do was to make a play on the receiver. I'd go as far as to say that vast majority of NFL safeties make the same play Clark does. You are never going to pull up short of a potential TD pass because your corner may have deflected the pass. From Clark's angle of attack, he may have been able to discern Ike Taylor was in the vicinity of the pass, but not whether he was able to make a play on it.
To suggest that Clark went out of his way to knock Cruz down egregiously is stupid. Clark is a veteran enough player to know that to do so would only cost his team points. If anything, Clark should be commended on his adjustment to the new defenseless receiver rules.This isn't the first time he's been wrongfully penalized this year either. Remember, the call on the field was a "blow to the head." The same blown call was made in week 2 against the Jets, when Clark popped Santonio Holmes, forcing an incompletion.
Not only was Holmes not defenseless-as he had taken 3 steps with the football-Clark also never made contact with Holmes' head.
Remember when this was legal/or at least accepted by executives high in the league?
The sudden 180 midway through the 2010 season on what constituted an illegal hit to a defenseless receiver certainly had plenty of Steelers unwilling to so quickly change their style of play. Clark in particular had a few hits last year that resulted in mail from the NFL, but it's apparent that he's made a conscious effort to change his style of play, the sad part is, the NFL doesn't care. Clark himself said following the Giants game that he feels refs meet before games to discuss targeting him specifically. While I can't drink that much Kool-Aid, I will say they penalize him with an obvious prejudice. Clark gets no benefit of the doubt.This is not to say he hasn't ever made an illegal hit, but it's becoming clear that he has made adjustments to his style of play. It's about time the NFL make adjustments to their officiating.


text:
Yeovil Freewheelers celebrates 35 years of service (From Bridgwater Mercury)
Get involved! Send your photos, video, news & views by texting MERCURY NEWS to 80360 or email us
Yeovil Freewheelers celebrates 35 years of service
Updated 3:23pm Tuesday 14th May 2013 in News
THE blood bike charity, Yeovil Freewheelers, is celebrating 35 years of service to the community.
Founded in 1978 by Mr Cecil "Ces" Turner and funded entirely by public donations, using volunteer riders, it continues to support Yeovil District Hospital and it's caption area covering South Somerset and North Dorset.
The charity's Chairman, Martin Surrey said: "Yeovil Freewheelers is the longest running blood bike charity in the UK, something we are immensely proud of.
"We wanted to make this a special year and take this opportunity to thank Cecil for all his past work in setting up the charity and his on-going interest and support of us."
The charity made a surprise presentation of a birthday cake to Ces while he was introducing the line up of motorcycles at this years Abbey Hill Steam Rally, followed by a lap of honour as pillion on one of the blood bikes.
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subset: commoncrawl


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Another fire at the Moorhead American Crystal plant is causing people to wonder if the plants are safe without the union employees. On August 1st, 1300 American Crystal union members were locked out of their jobs after a contract dispute.
The fire happened around 4:30 Thursday morning in the pulp dryer and spread to the room next to it. No one was injured but there was a considerable amount of damage.
This, along with all the other fires, makes Ross Perrin and other union members shake their heads.
"It's kind of strange. Six fires out of the valley and the equipment is being monitored by people running it," says union member, Ross Perrin.
"The pulp dryer at crystal sugar had a fire inside it and the extinguishing system that's in it had gone off and they were having a hard time getting some of the hot spots off," says Moorhead Fire Marshal Rich Duysen.
The pulp dryer is one of the most fire prone places in the plants and there's always someone there to watch it.
"There's a control room where there's an engineer I believe he's an engineer that would sit and monitor a lot of controls," says Duysen.
This is the second fire at Moorhead's plant since the lockout began. In the past five years, there has only been one other fire that's required a fire department response.
Duysen says "They've had a really good safety record over the years."
We called the fire departments in all five communities with plants. The numbers in Dryaton, Hillsboro and Crookston haven't changed. However, East Grand Forks has had one fire since the lockout... and only a total of two in the past five years.
"They'll write it off as a common occurrence. Any situation you know that you have like this can turn into a devastating event. Lucky nobody got hurt," says Perrin.
There is no significant difference in the amount of fires at American Crystal plants around the valley with the exception of Moorhead's having two. However, that does not show if it is at the fault of the replacement workers.


text: Never Stop Training 365 is the official blog of SOTL Training. The purpose of the blog is simply to make you better. Better than the guy ahead of you on the depth chart. Better than the opponent waiting for you on the other side of the line of scrimmage. Better than the guy your recruiter is comparing you to right now on film. NST 365: NEVER STOP TRAINING.


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Portable heaters: Compare features & prices DeLonghi fan heater HVF 3553TB review http://www.which.co.uk/home-and-garden/heating-water-and-electricity/reviews/portable-heaters/delonghi-fan-heater-hvf-3553tb/review/
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Bomb Threat Grounds Air France Flight
A December 15 Air France flight was held on the ground in Venezuela after French intelligence officials received a credible tip that a bomb would be detonated when the flight was over the Atlantic between Caracas and Paris. An extensive search of the Airbus A340 found no explosive devices and the flight was allowed to proceed.
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By RUTH EGLASH
Unlike their parents, the younger generation of Ethiopian-Israelis will not stay quiet about racism.
Ethiopian protest Kiryat Malachi
Photo: Marc Israel Sellem
Referring to them also as "
," or cockroaches, a tenant from an apartment block in the southern town of Kiryat Malachi stated proudly that he would never sell his home to an Ethiopian family because "it brings down the value of the neighborhood."
The man revealed that 120 other families spanning four residential buildings had collectively signed an agreement with the neighborhood council not to sell or rent their apartments to families of Ethiopian descent.
The news report was only the tip of the iceberg. In the week that has followed the broadcast, members of the Ethiopian community have galvanized their people, especially the second generation of immigrants, to speak out against a phenomenon that fails to abate and that spreads far beyond Kiryat Malachi.
"People always tell us that it will take time; they tell us to be patient and that it will take another generation for us to feel part of Israeli society." says Ethiopian activist Elias Inbram. "But I have lived here for more than 30 years, I have a law degree and a master's degree, I served in the army, so what else do we need to do in order for people to accept us?" Inbram, who also spent time studying in Chicago, was one of many highly educated and articulate young Ethiopian Israelis that came out Tuesday to protest the discriminatory housing policies in Kiryat Malachi and what he calls "institutionalized racism."
"This is not something new. We have seen it in so many areas: in the education system, in the job market and in housing," he says. "We keep hearing a lot of promises [from the government] that things will change but no one does anything."
"Even though we are talking about something specific that happened in Kiryat Malachi, this kind of racism against Ethiopians happens everywhere in Israeli society," observes Efrat Yerday, spokeswoman of the Israel Association of Ethiopian Jews (IAEJ), who also joined in Tuesday's protest.
Yerday, who was born in Israel to Ethiopian immigrant parents, adds that "it stems from similar attitudes within the political system, the education system and even within the police force. It is everywhere and this is just another event in a series of events."
Both Inbram and Yerday – who not only participated in the anti-racism protest in Kiryat Malachi on Tuesday but also joined other Ethiopian activists in the Knesset on Wednesday for an emergency hearing in the Committee for Aliya, Absorption and Diaspora Affairs – say that the second generation of Ethiopian Israelis (those who came as young children or who were born here) is no longer willing to sit in silence and suffer from ignorance or racism like their parents' generation.
"We can no longer sit on our hands and do nothing when we are confronted with this kind of racism," concurs lawyer Itzik Dessie, executive director of Ethiopian legal rights organization Tebeka, which has already started to lobby parliamentarians to create specific legislation to criminalize racism, including harsh punishments for those who are verbally racist.
He says that in the past, the community tried to be patient and opted to simply ignore "stupid comments" about Ethiopian immigrants.
"We would just say, 'oh, there is another idiot saying something stupid, it will pass,' but today we are seeing that it is not passing. It is not only about individuals in the street who are making racist comments but people in politics and other areas of public life too," Dessie laments, adding that the next generation is ready and willing to take up the battle in a way that has not happened before in Israel.
"We are not afraid to speak out against racism anymore," he continues. "We want to see change. We are sick of the situation and now there are tools such as Facebook that can reach many other young people all at once."
According to Dessie, the story in Kiryat Malachi is the straw that broke the camel's back.
"The fact that Ethiopians are prevented from buying buildings is not really the issue here; it goes much, much deeper," he says.
Indeed, just over the last few months, several incidents of blatant or unwitting racism and forced segregation of members of the Ethiopian community have been exposed by the media.
In September, attempts to close down a Petah Tikva public school with a student body comprising almost exclusively Ethiopian pupils put the spotlight on what the community calls "ghettos" – neighborhoods where new Ethiopian immigrants are forced to live due to limited financial means.
The incident also raised awareness to government housing policies that limit where the new immigrants can purchase accommodation. In order to receive a subsidized government mortgage, community members are forced to buy on certain streets and neighborhoods in only 10 specific towns. This restriction then creates a problem wherein some have an extremely large concentration of Ethiopian students.
A recent report compiled by the Israel Association for Ethiopian Jews found that more than 10 schools countrywide in which the number of Ethiopian pupils is more than 80 percent of the student body and a further 40 schools in which Ethiopians constitute 40%.
Another example of this problem was discovered last month in Beit Shemesh, where it was revealed that the municipality had forced the majority of Ethiopian children in the town to attend one of three kindergartens. The story was further exacerbated when Mayor Moshe Abutbul explained the policy by likening the Ethiopian immigrant community to small fish in a dangerous aquarium.
"In an aquarium there are big fish and little fish. First you have to take out the little fish in order to help them grown and stop them from being eaten by the big fish. That is how we are helping to better prepare Ethiopian children for first grade," he told local Israeli media.
"We need to change this situation and break down these ghettoized neighborhoods," notes Inbram, suggesting that money raised by international Jewry to support Ethiopian immigration and absorption could be put to much better use by focusing more on integration into Israeli society in a holistic way.
"Not only does there need to be a law against racism and efforts to enforce existing laws against discrimination but there also need to be better policies on integration in housing, education, industry and the public sphere," he says.
At Tuesday's protest, demonstrators expressed hope that tackling racism would now become a central issue in the struggle for social justice among mainstream Israeli society.
"We hope that this is the first step and that we can really make a change," comments 27-year-old student Shira Esayas, who traveled from Haifa to join the protest in Kiryat Malachi.
She continues: "We saw our parents being subjected to racism and they tried to protest it but no one listened.
Now we are here and if we do not speak out loud and clear then our children will also have to go out and fight racism."
Waving a banner with a well-known quote by Martin Luther King, Jr. – "Injustice anywhere is a threat to justice everywhere" – Esayas said that she had always been inspired by the changes King made in the US and is hopeful that this new stand for equality would achieve the same in Israel.
She points out that fight against racism is not exclusive to the Ethiopian community but rather a battle that "everyone in Israeli society should be involved in."
Avi Yalou, a resident of Kiryat Malachi and one of those who organized the protest this week, says that he too feels this is a battle relevant even to those outside the Ethiopian community.
Although few people – lawmakers, social rights activists or otherwise – from outside of the Ethiopian community joined Tuesday's anti-racism protest, Yalou says that "this is only the start of more things to come."
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text:
BEIJING (AP) -- U.S. Secretary of State John Kerry is in China on a difficult diplomatic mission.
Kerry is meeting senior Chinese officials on Friday in Beijing to seek their help in bringing a belligerent North Korea back to nuclear disarmament talks.
At the same time, he's demanding that China roll back a series of increasingly aggressive steps it has taken to assert itself in territorial disputes with its smaller neighbors.
Kerry faces a tough sell on both counts.
The extent of China's influence with North Korea is unclear following a purge in the isolated country's leadership.
And, China has angrily dismissed U.S criticism over its moves in the East and South China seas that have alarmed U.S. allies like Japan and the Philippines.
In Seoul, South Korea, on Thursday, Kerry made clear the Obama administration is putting new emphasis on getting North Korea back to stalled six-nation talks aimed at getting it to give up nuclear weapons.
"Let me be clear," Kerry said. "The United States will not accept North Korea as a nuclear-armed state. We will not accept talks for the sake of talks. And the DPRK must show that it will negotiate and live up to its commitments regarding denuclearization."
Efforts toward that end, he said, would rely heavily on China, North Korea's only friend, putting pressure on Pyongyang.
"China has a unique and critical role that it can play," he said. "No country has a greater potential to influence North Korea's behavior than China, given their extensive trading relationship with the North."
But China's leverage with the North is being tested.
Diplomats say Beijing received no prior warning ahead of the December arrest and execution of North Korean leader Kim Jong Un's uncle, Jang Song Thaek, who had been considered Pyongyang's point man on China affairs and was a strong promoter of free trade zones being set up along their mutual border.
That came on the heels of Pyongyang's snubbing of Beijing's wishes when it conducted a missile test in late 2012, followed by the underground detonation of a nuclear device last spring.
Jang's removal was seen as depriving Beijing of its chief conduit into the North Korean regime and in the weeks that followed the leadership found itself at a loss as to how to proceed. A delegation of Chinese diplomats led by the Foreign Ministry's deputy head of Asian affairs visited Pyongyang last week in a sign that Beijing was attempting to renew dialogue with Kim's government, although it remains to be seen whether the North was any more receptive to China's pleas to return to the nuclear talks.
Those discussions involving the two Koreas, the United States, China, Russia and Japan, broke down at the end of 2008 and U.S. officials say they see no point of restarting talks until Pyongyang shows an authentic desire to make good on its prior commitments to dismantle its nuclear programs.
Meanwhile, making the U.S. case for restraint and clarification on the competing territorial claims and China's bitter dispute with Japan may be even tougher for Kerry.
Since sometimes violent anti-Japanese protests rocked major cities in late 2012, Beijing has continually stepped up its rhetoric against Tokyo, dispatching its diplomats to make China's case in the global media and at international forums, even dogging Japanese Prime Minister Shinzo Abe's recent trip to Africa.
Recent weeks have seen China's ambassador to London compare Japan to the evil Lord Voldemort of the Harry Potter books in the pages of Britain's Daily Telegraph newspaper. On Thursday, the official China Daily newspaper devoted a half page to grievances against Japan, while the Foreign Ministry revived the case of a 2010 confrontation between a Chinese fishing boat and Japanese coast guard ships to demand an apology and compensation from Tokyo.
More worrisome, Chinese patrol vessels have maintained a more-or-less constant presence in waters surrounding the disputed Diaoyu islands in the East China Sea, forcing the Japanese coast guard to go on the defensive to avoid a clash.
Chinese ships have also stepped up their presence in the South China Sea, particularly in regards to the Philippines, which is seen by Beijing as weak and overly dependent on the U.S. for protection. Diplomats are concerned that Beijing may be planning to declare an air defense zone above those heavily traversed waters, further raising the chances of confrontation with American surveillance planes and other military flights.
U.S. officials traveling with Kerry said he would urge the Chinese to show restraint, cool down its rhetoric and actions, and clarify its claims consistent with international law.
"The perception in the region and in the United States that is generated by the incremental actions that China has been taking ... is one of a country that is asserting its position through extra-legal and non-diplomatic means," one official said. "That's not a good image of China, and it is not a pattern of behavior by China that the U.S. or others want to see."
(Copyright 2014 by The Associated Press. All Rights Reserved.)


text:
Remember Me
During the recent Michigan 4-H Air Rifle Postal shoot held in Amasa, three Iron County youths qualified for the 2012 Michigan Shoulder-to-Shoulder Tournament. Members of the Iron County 4-H Sharp Shooters are Joslyn Thoreson (left), who took fifth place in the junior division with a score of 328; Jesse Thoreson, (center) who took first place in the senior division with a score of 494; and Alex Nelson, who took third place in the senior division with a score of 479. (Marvin Hill photo)
AMASA—Three members of the Iron County 4-H Sharp Shooters have qualified for the upcoming 2012 Michigan Shoulder-to-Shoulder Tournament. Youth 4-H sport shooting clubs from across Michigan will be competing at the United Sportsman Club facility in Merriman. The event will take place on Saturday, May 19. First shooting relay will begin at 9 a.m. Iron County 4-H Sharp Shooters Jesse Thoreson and Alex Nelson will be competing in the senior division; club member Joslyn Thoreson will be in junior division. "Spectators are encouraged to attend and cheer on the local shooters," said Marvin Hill, club instructor.


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The Aprilia RX50 has the looks of a proper enduro bike, but none of the performance. Restricted, it'll struggle to break 30mph, and de-restricted (illegal for 16 year-olds) it'll top 50 eventually. The chassis will cope with gentle off-roading, but ride it harder and the RX50 will be out of its depth.
The Aprilia RX50 49cc two-stroke single engine is typical of the class – gutless. It needs revving hard and the clutch slipping to pull away, and you need to continue thrashing it. But as a first motorcycle, it'll feel lightning quick – it's all relative.
The RX50's chassis is realistically best around town – try ride it off road and you'll get sick of the suspension clanging in to its bump stops. The wide bars, lightweight and compliant suspension is perfect for urban riding, but the standard knobbly tyres will complain if you push too hard – buy the Aprilia SX50 with its supermoto-style road tyres if you don't ride off road.
The Aprilia RX50 is basic motorcycling – no storage, a riding position that makes no concession to comfort and a simple electronic dash displaying only basic information. There is a pillion seat – but only for the very stupid. The chassis looks nice, but is nothing special underneath the fancy façade. Compare and buy parts for the Aprilia RX50 in the MCN Shop.
Build quality is basic but OK – the main problem comes from abusive teenage owners. Cleaning, basic maintenance and using top-quality two-stroke oil should keep it happy for most of the time, but being both Italian and two-stroke expect the odd foible.
£2399 is a lot of money for a first bike that you're unlikely to keep more than a year. Insurance isn't cheap for a young rider – motorcycles like the Aprilia RX50 are towards the upper end of the risk scale compared with twist-and-go scooters. Find an Aprilia RX50 for sale.
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Year: 1997-2005. Uses an earlier version of the same engine, but in a steel chassis. The looks mimic Aprilia's 1990's 125cc enduro bikes. Very rare now – patchy build quality, abusive owners and bike thiefs looking for field bikes are responsible for the demise of most.
Aprilia SX50 2006-current. Much the same as the RX50 but with 17" road wheels and tyres and a larger front brake disc. Unless you really must ride off-road there's no reason to buy the RX50 – the SX50 offers a much better ride on the road.
Small engine, big bike!
Average rating
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Had this bike from new for 6 months now - and I love it!! Picked up a Gianelli exhaust as, although it was restricted to 30, it would struggle to get to 30, let alone maintain it. Now it will happily maintain 40mph no problem. The powerband is quite pokey and will out accelerate cars when filtering up until 30 or so.
The only gripe is that the seat is NOT comfy for prolonged periods! Reminds me of my mountain biking days...
There is also NO storage anywhere, so be prepared to use a rucksack, should you need anything.
No reliability issues at all. Mine hasn't had an easy life: dropped while off-roading, knocked into neutral while attempting to change up into second several times resulting in maximum engine revs and is either full throttle or nome at all due to my 'exuberant' riding style. Still going strong.
Great styling as well.
Will always stay with Aprilia (as long as this one serves me well for the next 4 months (which I'm sure it will)) and looking at an RS4 125 next as I've always wanted a sports bike. Got this one due to the manual gearbox, and not the RS50 because that's a 2 - stroke sports bike and that's just not cool!
20 July 2013
Best 50cc around
Great value for money. can hit 55 on straights (de-restricted) and is so reliable. great handling too!
13 August 2012
easily the best 50 cc going
jacob fraser
my aprilia rx has never let me down, it may only be a 50 but fells like it should be bigger it is a joy to ride in what ever wheather. the engine is solid and it is fairly quick for a 50cc. i have topped at 55mph on the flat a 70mph down hugely massive hills. if i was to ever go back to a 50 it would be an aprilia rx
11 August 2011
Good Points - Brilliant Whilst Working, Engine Good, Handling Good, I have had 54mph out of my fully derestricted stock SX50. Parts, take a while, and you will be lucky to go a year without neading to order and wait for any. The speedo is good but the rev counter doesnt tell you how many revs your doing just has a bar that goes up and down. Overall, a brilliant little bike and is a great head turner. Get a new model 2006 - current and if you are 16, it is guarenteed that all you mates, bike lovers or not will want one. Just remember you cant have it all, speed and style are the pluses, typical italian reliability - poor and bad parts service.
11 November 2009
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Updates and Confirmations from the SEO Top – SMX West Updates
SMX West didn't bring many new things to the table, but it did explain a few things better for everyone to understand, especially regarding the Farmer/Panda update. We have Matt Cutts and Danny Sullivan and other SEO experts to thank for that. You can read the detailed post on Search Engine Land if you want, […]
Is Your Facebook Fan Page Firing Blanks?
Guest post by – James Brack (www.dreamscapedesign.co.uk) Hearing about the role Facebook can play in your marketing strategy has almost become a cliché, and has driven many business owners into a state of panic causing them to blindly create a fan page without any strategy. This can clearly be seen in the Facebook graveyard, consisting […]
Page Rank Update, Good News for Some, Bad for Others, Irrelevant for a Few
Most of you have heard about the Page Rank update in the last few days. For some this has been a good event, for others still biting on their nails, waiting when the update is going to hit their website this might seem like a nightmare. For those in the SEO industry this is just […]
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SPRINGFIELD, Ill -- State Representative Mike Unes said Tuesday in a statement that he is hopeful for a House vote by the end of the 97th General Assembly on his bill to aid Wildlife Prairie State Park's financial uncertainty.
Unes is a sponsor of a bill that would transfer the bill from the state to not-for-profit group Friends of Wildlife Prairie State Park.
"It is extremely important to the region to keep a gem like this open, not to mention it is a huge relief to the state to not have to pay the more than $6 million that is needed in capital improvements to the park," Unes said.
The bill passed unanimously in the Senate and now only needs a vote of concurrence in the Illinois House to be sent to Governor Pat Quinn.


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Gonzaga Prep solidified its GSL supremacy Thursday night, putting away a tough Mead team 71-55 in a game that was a lot closer than it appears by looking at the final score.
First-place Prep (17-1, 12-1 GSL) was looking for a bit of revenge against the Panthers, given that it was Mead (13-5, 11-2 GSL) who handed them their only loss of the season in early January – a 52-53 loss at the hands of Bo Tully.
The Bullpups would not be denied this time. The game was back and forth up until the fourth quarter when Prep's Parker Kelly hit back-to-back three-pointers from the left wing to break the game open.
Ryan Nicholas, who committed to play at Portland next year, scored a game-high 28 points and fought off double and triple coverage all night long. Chris Sarbaugh added 15 for the Bullpups and Kelly scored 10.
Mead's Aaron Dunn, who signed a letter of intent yesterday to play football at Washington State, scored a team-high 24 points for the Panthers, matching Nicholas' presence inside for most of the game. Bo Tully scored 11 and both Brandon Meyers and Taylor Farnsworth scored 7.


text: PLYMOUTH NAVY STRENGTH GIN – The Navy Strength version of Plymouth Gin offers an amplified aroma and fragrance of botanicals while retaining the brand's signature smoothness and balanced character. First introduced to the Royal Navy in 1793, Plymouth Gin is produced in the oldest working gin distillery, and the only major distillery left in Plymouth, London. POS materials will be available to support the launch. Available from Horizon Beverage Company and MS Walker.


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A man paralyzed for four years can now stand and move his legs thanks to an implanted electronic device that stimulates function -- and perhaps growth -- of nerve cells in the spine.
Click here to read the full story.
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Maple Ave & West St has a Walk Score of 72 out of 100. This location is Very Walkable so most errands can be accomplished on foot.
The closest park is Veterans Memorial Park.
Nearby schools include Barrington High School, Nayatt School and St Andrews School.
The closest grocery store is Shaw's Supermarket.
Nearby coffee shops include Barrington Kangaroo Cafe, Dunkin' Donuts and Starbucks. Nearby restaurants include Trendy's Pizza, Mangia Pizza and Miku Sushi Restaurant.


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Brezhnev Republicans
by Intrepid Liberal Journal, Fri Apr 28, 2006 at 07:44:39 PM EDT
The metastasizing Jack Abramoff scandal exposes the Republican Party as a centralized cabal of apparatchiks and commissars. They are a monolithic greed factory oozing with corruption and contempt for public service.
Once upon a time Republicans were guided by firm ideological convictions: reduce the size of government, strengthen national defense, and develop a culture of personal responsibility. Those convictions are now mere slogans designed to preserve the party's brand label and maintain power.


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A new reading program plus an extra hour in the school day are paying off in two ways at Springfield Elementary School. The school saw a significant increase in FCAT reading results this year compared to last, and now it's earned a national award for its achievements.
The Association of Direct Instruction is giving Springfield Elementary the 2013 Wesley Becker Excellent School Award. Wesley Becker was a founder of direct instruction, a teaching method where teachers sit with a small group of students and instruct them one-on-one.
Springfield was the only school in Bay County required to add an additional hour of reading instruction to the school day for the 2012-2013 school year.
The hour plus some extra funds allowed the school to implement a new reading program. Principal Harriett Taylor said what the school was doing before the new program wasn't working, and this award just solidifies the hard work in improving reading.
"I'm just very proud of the teachers, the paraprofessionals, students, and parents for all their support in going into this program and implementing it, with fidelity. They've all done a great job. The teachers, paraprofessionals, and students are to be commended for all their hard work," said Taylor.
Springfield will also receive a plaque, and a $1,000 cash reward. Principal Taylor said she's going to let the teachers and staff decide how to spend the money.
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An uncorrupt and just person who grew out of corrupt beginnings
In both Hollywood as well as his childhood, he remains uncorrupted while staying to the true path. As a result, he has gained true strength.Explore posts in the same categories: Culture, Traditional Martial Arts
One Comment on "An uncorrupt and just person who grew out of corrupt beginnings"
February 12, 2013 at 5:43 pm
[...] mentioned before that this person had true strength. What did I [...]


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Remember Me
Traffic passes utility crews working along M-189 as preparations for this summer's road project continued recently. Work on rebuilding the highway starts this week.IRON RIVER—Preparations are nearly over. The M-189 project between Genesee Street and Hiawatha Road starts this week. A public information meeting will be held Tuesday, May 14, at 5 p.m. at Iron River's City Hall to discuss the project and temporary changes that will be in effect during the project. Citizens and business officials, especially those living near M-189, are invited to attend and ask questions.
MDOT officials will be at the meeting, along with representatives of Hebert Construction, which is the general contractor. Dave Bradley, the project's design engineer for MDOT, said there will be a brief presentation about the project, and after that it will be a question session, both as a group and one-on-one. "A lot of it is about schedule: start dates and end dates and how traffic is impacted," he said. "That's the kind of stuff people usually ask." This is a 1.15-mile project on one of Iron County's busiest highways, and work should continue into October. By the time it is over, the roadbed will have been rebuilt, and M-189 will be a three-lane highway, the way U.S. 2 is through downtown Iron River. The highway will also be realigned for several blocks between Ross and Maple streets to remove the infamous double turn on Fourth Avenue near the credit union building. Contractors and utility companies have been working along the highway for months in preparation for the actual start of construction. The overhead utility lines on the west side of the roadway are being shifted to new poles further west. M-189 will remain open throughout construction, and the east side of 189 will be widened temporarily south of Ross Street. North of Ross, parallel one-way streets will be used as a detour. Northbound 189 will take Ross east to River Avenue and north to U.S. 2. Southbound 189 will use Third Avenue between U.S. 2 and Ross. South of Ross, flagmen will regulate traffic near the construction zone during the day--the road will be two-way at night, though one side of the roadway may have a gravel surface. Most of the highway will have two-way traffic throughout construction, allowing access to businesses and homes. Temporary signals will be used near where a large culvert is being replaced, just north of Hiawatha Road. That is at the south end of the project and will be one of the first things done. "They'll work their way up the road," said Mike Premo, MDOT's Crystal Falls office manager, "and when they get so far, then they'll pave half. At some point, we'll switch traffic to the new side and do the old side." At the same time, the city of Iron River will be replacing some sanitary sewer lines and service lines between Genesee and Division streets, and water line work will be done at the Genesee Street intersection. Planning for the project has been under way since 2006. The goal of the project is to make M-189 a better road by removing old concrete surfaces; replacing the silty roadbed, which contributes to frost-heave; and eliminating the double curve near the credit union building.
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NEWARK, Del. (AP)- Newark police have charged a Wilmington man with burglary and other offenses after they say he was seen with lingerie and crouching outside a home.
Officers were called about 1:30 a.m. Wednesday to Choate Street after a passerby saw a man near a first-floor window. Police say responding officers saw women's lingerie hanging from the waistband of the man's pants.
Authorities say the man fled and an officer deployed a stun gun, striking him.
Thirty-nine-year-old Sean Maas has been charged with burglary, trespass with intent to peep or peer. Police say Maas had gained entry to a home and stole the lingerie.
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Paul Blanchard's outside shooting kept it close, but Staten Island Academy dropped a hard-fought, 60-56 decision to Leman in ACIS playoff action on Wednesday.
The win drops the Tigers' record to 14-9 and coach Mike Mazella's squad will have to wait until Sunday to see if they're afforded a NYSAIS State Tourney bid.
"It was a close game throughout, a typical No.4 vs. No. 5 seed game," said Mazella about the matchup. "We played hard, we just had a few missed assignments and it hurt us."
The Tigers played the Manhattan school evenly through three quarters, trailing by one entering the final frame.
That's when Blanchard got hot, draining three of his three-pointers to keep SIA in the game.
"Paul stepped up, he had a big fourth quarter," said Mazella. "And James Moriasano had a solid game for us. He's been sick all week, but he still managed to score 19 points. He gave us all he could."
Trailing by three late in the game, the Tigers had a chance to tie but turned the ball over with 14 seconds remaining.
"We made the clutch plays with three and four minutes left," said Mazella. "They made the clutch plays in the final minute."
"When you play a team that is this evenly matched with you, it comes down to just a couple of plays," continued the SIA coach. "We just didn't make enough plays down the stretch and they did. It came down to a couple of possessions."


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August 21, 2014
Why You Should Eat Yak Instead of Beef
Posted on Sep 27, 2011
I first comprehended the awfulness of the ranching industry when the cows of Moonlight Meadows, high in the La Sal Mountains of Utah, surrounded me and began shitting. They had been trucked in from the canyons of the lowland desert countryside, near the town of Moab, to graze summer long on the free grass in the mountains—public grass, as this was land protected in trust for the American people by the federal government, under the auspices of the U.S. Forest Service. But none of it was much protected; the feces were the visceral evidence of this.
Along the trail the cows had laid big diarrheal pies, ice slippery, landmine-like, which splashed onto socks, legs and shorts. Where the trail steepened, the shit was treacherous. Soon I was surfing in it and finally was thrown like a clown on a banana peel. I lay there feeling stupid and looked around at the hundreds of cattle in the field nearby, one of many herds strewn in the mountains. A mother turned her ass to me and fired a big green load of diarrhea. Another cow pissed in an arc like a fire hose.
The cows evacuated thus onto the land and into the air, sometimes onto one another's backs and into eyes, the shit where it had dried swirling in dust devils when the wind kicked up and where it was sloppy and wet running in streams, in puddles, in steaming black pools. The stench was ammoniacal, sulfurous, and as I lay there in my dung-splash wallow, it was, quite suddenly, an unbearable experience: not just the smell of bowels evacuating, of creatures destined for the slaughterhouse as death on the hoof, but the cultural and political travesty it represented, the ecocidal meaning of it.
Cows are terribly destructive creatures, especially in arid climates. Livestock are considered by a quorum of scientists as the No. 1 cause of species extinction, topsoil loss, deforestation and desertification in the American West. They muck or stomp or gorge out of existence streams, whole watersheds, rare grasses and shrubs, entire ecosystems in micro. Their big heavy hooves trample the soil, eroding it often beyond repair. Just as the cow is an invasive species, an exotic in the West—an import of Spanish missionaries in the 16th century—it brings invasive weeds that triumph in its midst: the water-greedy tamarisk, for example, along with the greedier Russian olive and the useless Russian thistle, better known as tumbleweed. A 1998 study from the Journal of Arid Environments found that a hundred years of livestock grazing on public lands near the White Sands Missile Range in New Mexico was more damaging in terms of long-term development and recovery of flora than multiple nuclear bomb blasts.
More than 300 million acres of public lands in 11 Western states—lands owned by the National Park Service, U.S. Forest Service, the Bureau of Land Management, the U.S. Fish and Wildlife Service and, collectively, by all Americans—are given over to the resource destruction of the cattle industry. That's 40 percent of the landmass of the West, 12 percent of the landmass of the lower 48 states. The ranching industry controls this domain with massive subsidies from state and federal government. Even private insurance lenders such as Metropolitan Life, Mutual Life and Prudential, which in recent years have issued more than $1 billion in questionable loans to ranchers who used federally issued grazing permits as collateral, are now silent players in the subsidized ruination of Western public lands.
Square, Site wide
Democratic and Republican Congresses alike have defended these subsidies as the cowboy's divine right, though in fact federal grazing provides just $1 out of every $2,500 of taxable income in the West, and just 1 out of every 1,400 jobs. Ranching on public lands in the West contributes minimally—less than 3 percent—to national beef production, as most American beef is husbanded in the temperate East. Meanwhile, the industry in the last 20 years has consolidated into fewer and fewer hands, those rich operators who can survive the exigencies of the globalization of the beef trade and the rampant land value speculation that has overtaken much of the scenic West. Today, 10 percent of the cattle operators in Montana, to take just one example, own 50 percent of the cattle in the state. These new cattle oligarchs, lobbying government to maintain their power, are the modern incarnations of the legendary barons who helped win the West in the 19th century via coercion of whole cities, corruption of officialdom, collusion with railroads and Eastern banks, rampant violence, the ecocide of buffalo and the genocide of Native Americans. The power of political cowboyism today extends even to cowing the big green conservation groups—the Wilderness Society, the Sierra Club, the National Wildlife Federation, the Natural Resources Defense Council—that have refused to tackle head-on the environmental damage from ranching.
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By garyl, October 2, 2011 at 4:39 am Link to this comment
Before we get too enamoured of Polyface Farms… Visitors to the place have reported horrid conditions, including rabbits in sweltering hot metal cages suspended above manure pits. Polyface uses chickens that have been bred to grow so fast that they're adult size at onl 7 weeks old - still babies. The hyper-fast growth rate puts enormous strains on their organs and skeletal systems. The chickens - as on factory farms - are denied their usually doting mothers and fathers.
Polyface claims to be efficient, but when you factor in the trucked in chicken feed, that claim is washed away.
Like virtually all farms in the West, the animals at Polyface have been bred to overproduce flesh, eggs, or milk, and they are denied most of the lifespans.
The factory farms and overgrazing that everyone hates are a result of demand for cheap meat, milk, and eggs. As long as we're killing 25 million "food" animals every hour in the US alone, factory farms and overgrazing are nearly inevitable. And our appetite for fish is decimating fish populations, to say nothing of the huge toll on "bycatch" species.
Rather than pretend we're being humane when we kill for pleasure or profit - the very antithesis of humaneness - let's get out of the mass-murder businesss. Studies such as EPIC-Oxford show that vegans fare quite well, and for most people in the first world, it's an easy switch, once you get past the psychological barriers.
The notion that we need to kill domesticated animals is incorrect. Veganic gardening is taking off and shows great promise, and the Hunzas and Native Americans are just two of the populations that have gotten high crop yields without using domesticated animals. Moreover, it's doubtful that the huge monocrops fed to livestock will ever use manure, as it's too impractical at that scale. Lastly, if it truly were necessary to use domesticated animals (usually non-native and human-engineeered in practice!) to raise crops, it doesn't follow that we have to kill them as soon as they're full-grown.
By buduranusx2, October 1, 2011 at 4:49 pm Link to this comment
By Lennerd, October 1, 2011 at 3:40 pm Link to this comment
So, my plan to open a chain of fast-food burger joints in Tibet and eventually the whole world is on track: Yak-in-a-Box.
By Mongol Reader, September 30, 2011 at 4:30 am Link to this comment
Here in Mongolia yaks are one of the major domestic
It is a DISASTER. The desertification is out of
This is why in only three years, the number of people
Join Us Please. Do it for reasons of compassion for
By cwbystache, September 29, 2011 at 4:01 pm Link to this comment
Maybe the author should stop "yakking" long enough to read what other scientists say outside this mysterious "quorum" he mentions ... "Ranching, Endangered Species, and Urbanization in the Southwest: Species of Capital" by Nathan Sayre of UC Berkeley (he also wrote, "Working Wilderness: The Malpai Borderlands Group and the Future of the Western Range") would be a start; there are many others. I wonder what Christopher Ketchum thinks of The Center for Biological Diversity's lawsuit to protect that very weed he hates and whose spread he blames on cows, the tamarisk? Seems the organization that is the quintessential hater of the American Cowboy and Cow now considers the "water-greedy" invasive an essential component of the riverine ecosystem of the aridlands if the subspecies, Southwestern Willow Flycatcher, is to continue in existence (see, "Eradicating Tamarisk Trees May Endanger a Bird Species" http://www.fronterasdesk.org/news/2011/sep/05/tamarisk-river-environment-non-native-plants/). There is quite the bibliography of works by scientists and working ecologists that might lead one to a different conclusion than this author's "quorum". More and more I think that that what all this is really about, though, is that I get to wake up every day out in these wilds, be with the wildflowers and the migrating warblers (which I can identify down to species by voice alone) and ibis and hummingbirds, hear the Poorwills at night, be horseback and view the canyons and the prairies while I look for strays, smell the pines or the creosote bush (depending on what season, and where I'd be cowboying)—and, well, Christopher Ketcham doesn't get to, and since he can't, I expect he doesn't think anyone else should be allowed to. Yes I raise cattle, no, I don't see the post-cowpocalyptic world he here describes though something worse appears the closer I might drive towards the kind of urban area so many armchair "environmentalists" inhabit and from whose messed-up-forever environment they launch their brickbats at me, now they're comfortable in and can take advantage of a completely artificial world that came to them at the cost of destroying a once-marvellous environment that existed under their house or apartment building. I do often slip in cowshit like a clown on a banana peel the way Ketcham has, though.
By BS, September 29, 2011 at 7:52 am Link to this comment
I LOL'D. Nice opening.
By suzanner, September 28, 2011 at 11:07 pm Link to this comment
You forgot to mention another "undesirable" wild animal that is being decimated
Only in America do we ship wild horses to cattle pastures and feedlots and let the
By Memory Stick, September 28, 2011 at 11:11 am Link to this comment
Scientists have learned that the active ingredient in Cannabis Oil kills many cancer cells:
Hemp Seed is high in protein and Hemp Oil has Omega 3 acids, the deficiency of which is one of the big reasons so many Americans age early and with so many health problems
Hemp can be used to build a house, feed the inhabitants, clothe them and provide gas for their engines (the first diesel ran on peanut oil)
The USS Constitutions sails were made from Hemp.
In 1942 the US Government made a film called "Hemp for Victory" to motivate US Farmers to grow hemp during the war. (it's on Youtube)
Many people prefer to remain ignorant and/or ridicule others when finding out what they've been told all their lives has been a big helping of balderdash.
It is fun to watch!!


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Genetics Home Page --> Laboratory activities --> 2D studies
The megagametophytes and embryos were collected from non germinated seeds and were individually crushed in µl/mg of 3M urea, 4% FSN - 100 (ZONYL Fluorosurfactant, DuPont), 2% ampholytes (Pharmalytes 3 - 10) and 1% dithiothreitol in a 1.5 ml microcentrifuge tube (Anderson et al, 1985; Bahrman and Damerval, 1989). The mixture was briefly sonicated and extracted for 1 h at room temperature. After a brief centrifugation at 15000 g during 2 min, the supernatants were removed and stocked at -20°C until isoelectrofocusing. The megagametophyte from a germinated seed was collected just before the germinant is ready to cast its seed coat. The seed coat still contain the residual megagametophyte inside. They were individually extracted in 6 µl/mg of UKS (9.5M urea, 5mM K2CO3, 1.25% SDS (sodium dodecyl sulphate), 0.5% dithiothreitol, 2% Pharmalyte pH 3-10, and 6 % Triton-100) buffer (Damerval et al, 1986) and the supernatants were stored at -80°C after centrifugation at 15000 g for 2 minutes. Secondary needle and bud proteins were extracted according to Damerval et al (1986). Liquid nitrogen powdered - tissue was homogenised with 10% TCA (trichloroacetic acid) and 0.07% 2-mercaptoethanol in acetone. Proteins were precipitated for 1h at -20°C. After centrifugation at 15000 g for 15 min, the protein pellets were rinsed with acetone containing 0.07% 2-mercaptoethanol for 1h at -20°C. The supernatant was removed and protein pellet vacuum-dried and solubilized in 15µl/mg of UKS buffer. The pollen proteins were extracted directly by UKS buffer, using 30µl of UKS per mg of pollens and the supernatants were saved after centrifugation at 15000 g for 5 minutes. The quantities of extract to be solubilized in UKS buffer was determined on the basis of protein pattern comparisons in these different tissues. Our goal was to obtained similar amount of proteins for 2D gel comparison. The isoelectrofocusing (IEF) rod gels were 24cm long and 1.5mm diameter. The mixture was 4% acrylamide, 9.2M urea, 2% Triton X-100 and 4% ampholytes (3/4 pharmalyte pH 5-8, 1/4 pharmalyte pH 5-6). The IEF was performed at 40000 Vh with 50mM NaOH and 50mM H3PO4. The 2nd dimension electrophoresis was realised on slab gels (200x240x1 mm) bound to Gelbound PAG (Marine colloids) in a Dalt tank. Uniform gel composition was 11% acrylamide, 0.5M Tris-Cl-, 0.15% SDS, and 1% sucrose at pH 8.8 (Bahrman and Damerval 1989). The running buffer was composed of 0.025M Tris, 0.192M glycine and 0.1% SDS. The gels were simultaneously run and silver stained according to Damerval et al. (1987) in the apparatus described by Granier and de Vienne (1986).
DNA was extracted using the CTAB method (Doyle and Doyle, 1987; Bousquet et al., 1990) with some modifications. Megagametophyte tissue was ground to a fine powder in 1.5 ml microfuge tube under liquid N2. Preheated 2% CTAB buffer (700 µl) that contained 0.2% 2-mercaptoethanol and 0.5 mg/ml proteinase-K, was added to the powder, then the mixture was homogenized and incubated in a 65°C water bath for 30 min. After the solution cooled to 25°C, 500µl of 24:1 chloroform:isoamyl alcohol (CIA) was added. After centrifugation (12,000 x g ; 5 min ; 25°C), the upper aqueous layer was transferred, extracted with 1/10 volume of 10% CTAB in 0.7 M NaCl, and the CIA step was repeated. After centrifugation, the DNA in the supernatant was precipitated with an equal volume of isopropanol (500 µl). The nucleic acid pellet was collected by centrifugation (12,000 x g, 15 min, 25°C ), washed in 70% and 95% ethanol, dried briefly under vaccum and resuspended in 200 µl of TE buffer (10 mM Tris-HCl, pH 8 ; 1 mM EDTA) containing 10 µg/ml RNAase. The yield of DNA was approximately 2 µg per megagametophyte.
DNA from needles was prepared in a similar way. Freeze dried needles (25 to 70 mg) were ground under liquid nitrogen using a prechilled mortar and pestle, and transferred to 1.5 ml microfuge tubes. Samples were incubated for 45 min at 65°C in CTAB buffer that contained 1 mg/ml proteinase-K. DNA was extracted from freeze dried needles (4g) of both grand-parents and the hybrid parent using the same method.
The pine DNA was diluted to a working concentration of approximately 1 ng/µl by comparison with the fluorescence of lambda DNA concentration standards on ethidium bromide stained agarose gel.
The extraction of clean DNA was difficult because of the presence of a variety of secondary metabolism compounds and carbohydrates. Nucleic acids were isolated from dry or fresh leaves using standard methods (Saghai-Maroof et al. 1984) with some modifications. Approximately 300mg of dry tissue was ground under liquid nitrogen with PVP (Polyvinylpolypyrrolidone) and suspended in 9ml of preheated extraction buffer (100mM Tris-HCl pH=8.0, 1.4M NaCl, 20mM EDTA pH=8.0, 2% alkyltrimethylamonium bromide (CTAB), 0.2% b-mercaptoethanol). The homogenate was incubated at 65°C for 90min, cooled and emulsified with 4.5ml of dichloromethane and centrifuged at 1,600g for 10min. The aqueous phase was transferred to a new 15ml tube and emulsified again until a clear aqueous phase was obtained. The aqueous phase was then incubated 1h at 37°C with 30mg RNase A. DNA was precipitaded with the addition of an equal volume of isopropanol, dried and dissolved overnight in 1ml buffer (EDTA 0.1mM, ammonium acetate 10mM). DNA was precipitated again with the adddition of 1/20 volume of 5M sodium acetate and 2.5ml ethanol 95%. After 10min centrifugation at 1,600g, the DNA pellet was washed for 20min in 200mM sodium acetate, ethanol 76% and for 20min in 10mM amonium acetate, ethanol 76%. Finally, the pellet was rinsed with ethanol 76%, vacuum dried and resuspended in distilled water. The above method yielded between 15 µg and 20 µg of clean DNA. DNA concentration was measured with a fluorometer, diluted to a working concentration of 7.5ng/µl in sterile water and stored at -20°C .
(adapted from Doyle and Doyle, 1990)
-buds: remove scales from 5 medium-size buds. Cut the larger buds in two pieces.
-leaves: cut 2-3 cm² of preferencially fresh leaves into small pieces.
Roots or cambium can be used when buds are two small or leaves not available.
Put the material in microtubes (1.5 ml).
1) For 36 samples (depending on the capacity of the centrifuge), mix 40 ml of extraction buffer with 80µl of 2-mercaptoethanol (0.2% v/v) and add 250µl of this solution in each of the 36 microtubes.
2) For grinding, we use an electric driller with an home-made glass drill that fits within an Eppendorf tube. Between two samples, rince the drill with dH2O and wipe with paper. Wet the drill and cover it with dry sand. Grind while moving the tube up and down until you get an homogeneous mixture. Add 750 µl of extraction buffer and mix well. Put the tubes horizontally in boxes within the shaking incubator during 1 h at 55°C. Take the tubes out and wait 10 min until they cool.
3) Add 400µl of dichloromethane and mix gently until you get an emulsion. Centrifuge 10 min at 13,000 rpm (4°C). With a pipetman (blue tip), pipet the upper phase (avoid to pipet the interphase) and put it in a new labelled Eppendorf tube. If the upper phase is not clear, repeat this sequence again.
4) Add 400µl (2/3 volume) of isopropanol (let in the freezer since the morning) and mix gently. If the DNA pellet doesn't appear, put the tubes in a freezer 1 h at -20°C. Centrifuge all the tubes (with the pellet or not) 10 min at 13,000rpm (4°C). Remove the supernatant. Dry the tubes by letting them upside down on filter paper during 15 min on the bench.
5) Add 1ml of ethanol 76%, vortex and centrifuge 10 min at 13000 rpm (4°C). Remove the supernatant carefully and dry 15 min in a speed-vac at room temperature (if no speed-vac then dry longer on the bench).
6) Add 100µl dH2O. This is your stock solution. Estimate the DNA concentration and dilute to get a 100ng/l solution. Make a diluted solution at 2ng/l that will be your work solution.
Extraction buffer (1l)
ATMAB (Alkyltrimethylammonium bromide) 20g
EDTA 0.5 M , pH=8 40 ml
Tris HCl 1 M , pH=8 100 ml
NaCl 5 M 280 ml
Heat the solution at 65°C to allow the dissolution of ATMAB.
RAPD assay in Maritime pine
The RAPD reactions were performed following the method described by Williams et al., (1990). Primers were purchased in kits (OP-A through OP-Z) from Operon Technologies Inc. (Alameda, CA). The volume of the reaction mixture was 15 µl and contained 8 mg/ml nonacetylated Bovine Serum Albumin (New England Biolabs), 5 to 10 ng genomic DNA, 10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 0.1% Triton-X, 200 mM of each dATP, dCTP, dGTP, dTTP, 20 ng 10-mer primer and 1 U of Taq DNA Polymerase. The mixture was covered with 50 µl of mineral oil to avoid evaporation and amplifications were carried out in 96-well microtitre plates using MJ Research PT-100 thermal cycler (MJ Research, Inc. Watertown, MA). The reaction conditions were 41 cycles, consisting of 1 min/92°C (denaturation), 1 min/35°C (annealing) and 2 min/72°C (extension) with a ramping time of 1°C /3s. The DNA fragments were separated by standard electrophoretic methods (1X TBE) on 2% horizontal agarose gels, stained with ethidium bromide (0.2 mg/ml) and the gels were photographed.
RAPD assay in Eucalyptus and Oak
Oligonucleotide primers (10-mers) purchased from Operon Technologies Inc. (Alameda, CA) were used as single primers for the amplification of RAPD sequences (Williams et al. 1990). The reaction mixture (25 µl total volume) contained 67mM Tris-HCl pH=7.5, 2mM MgCl2, 1 ng BSA (Bovine Serum Albumin), 0.2% b-mercaptoethanol, 16mM ammonium sulphate, 200mM of each dNTP, 0.55mM of primer, 15ng of genomic DNA and 1U of Taq DNA polymerase (GIBCO BRL), overlaid with mineral oil. Amplification was performed in a thermal cycler model PHC-3 (Techne). The thermocycler programme was : preliminary denaturation (4min, 94°C ), followed by 35 cycles consisting of denaturation (45s, 92°C ), annealing (45s, 40°C ), and extension (1.5 min, 72°C ), and a final extension (10min, 72°C ).
The amplification is realised with 5 µl of DNA (~5 ng/l) and 20 µl of a PCR mix. A first denaturation (4 min at 94 °C) is followed by 30 cycles of amplification (the annealing temperature depend of the primers used in the PCR) and a final elongation (10 min at 72°C). The amplification products are stored in the fridge.
PCR Mix for one sample:
2Xbuffer 12.5 µl
Primer 1 (2 µM) 2.4µl
Primer 2 (2 µM) 2.4 µl
dH2O 2.65 µl
Taq polymerase (5 U/µl , Red Goldstar) 0.05 µl (i.e. 0.25 U by sample)
2Xbuffer (1 ml):
10X (provided with the polymerase by Eurogentec) 200 µl
MgCl2 (25 mM, provided with the polymerase by Eurogentec) 144µl
dNTP (5 mM for each dNTP) 40µl
dH2O 616µl
PS: MgCl2 is added in order to have a final concentration in the PCR reaction of 2 mM which was considered to be the optimal concentration for the amplifications.
5µl of the amplification product is added to a digestion mix. At the end of the reaction, 5µl of bromophenol blue is added and the samples are stored in the fridge when they are not immediatly analysed. The migration is realised on 8% polyacrylamide gels, 1.5 mm thick. The electrophoresis apparatus is the model SE600 of HOEFER. We use combs with 28 teeth (a microplate of 96 samples requires 4 gels for one primers-enzyme combination). The migration is made at 300V (the amperage for one gel is comprised between 30 and 50 mA). The gels are cooled at 15°C with a refrigerating system. After migration, the gels are stained with ethidium bromide.
|Digestion mix:
enzyme buffer 1.5 µl
enzyme (10 U/µl) 0.5µl
( i.e. 5U by sample)
dH2O 13 µl
|Acrylamide 40%:
acrylamide (Biowhitaker (Serva) 390 g
bisacrylamide (Sigma) 10g
|8% polyacrylamide gel:
H2O 26.25 ml
Acrylamide 40% 7.5 ml
TBE 10X 3.75 µl
APS 10% 180µl
TEMED 30µl
|TBE 10X(for 1 liter):
Tris 108 g
Boric acid 55 g
EDTA pH=8 (0.5M) 40 ml
Final pH=8.3
For the SSCP analysis, the digested PCR products (2 µl) are added to a denaturing solution (5 µl) containing 95% formamide, 10mM NaOH, 0.05% of xylene cyanol and 0.05% of bromophenol blue. After 4 minutes at 94°C, to separate the two strands, the solution is immediately cooled on ice. The single-stranded DNA fragments are separated in a 0.75mm x 16cm x 18 cm, non-denaturing Mutation Detection Enhancement (MDE) gel (0.5X MDE, Bioprobe Systems; 0.6X TBE). Electrophoresis lasts 16 to 18 h at 7.5 to 10 V/cm at a constant temperature of 15°C in 0.6X TBE buffer. The gels are then silver-stained with the method of Bassam et al. (1991). After fixation with acetic acid 10% during 20 minutes, the gels are impregnated with silver nitrate and formaldehyde.The gels are then rinced with deionized water (3 times during 2 minutes). Colour development is obtained with sodium carbonate, formaldehyde and sodium thiosulfate and the reaction is stopped with acetic acid 10% during 10 minutes. The gels are finally rinsed with deionized water during 10 minutes and dried between plastic sheets .
|colour impregnation (30 min):
silver nitrate 0.1%
formaldehyde 0.056%
|colour development (5 to 10 min):
formaldehyde 0.056%
sodium thiosulfate 2 mg/l
Microsatellites or simple sequence repeats (SSRs) are highly mutable loci which may be present at many sites in a genome. Since the flanking sequences at each of these sites may be unique, if SSR loci are cloned and sequenced, primers to the flanking regions can be designed to produce a sequence-tagged microsatellite (Tautz, 1989).
Microsatellite primers used for the two species Q.petraea and Q.robur were developed by Austrian colleagues (Steinkellner et al., Plant. Mol. Biol., in press.).
Reactions are performed in a total volume of 25µl, included 0.2 µM of each primer, 67 mM. Tris HCL, pH 8, 1 ng. of BSA, 0.2% mercaptoethanol, 16 mM. ammonium acetate, 100µM of dNTP and 0.8 unit of Taq DNA polymerase (Gibco BRL). Amplification proceeded first with one denaturing step of 94°C for 4 min. and then with 30 cycles of 94°C for 45 s., Ta for 30 s. , and 72°C for 30 s..
PCR products are then mixed with a stop solution ( 95% Formamide, 0.05% Bromophenol Blue, 0.05% Xylene cyanol, 10µM NaOH) and denaturated at 94°C for 4min. before being resolved on 6% standard denaturing polyacrylamide gels : 42 g urea, 15 ml 40% acrylamide, 10 ml 1X TBE, 100 ml qsp. deionized water. For the following silver staining, Bind Silane (PHARMACIA) is applied on one of the plates of the sequencing electrophoresis system before pouring the gel.
The run parameters depend on the equipment (length and thickness of the gel). It should be performed at constant Watts to reach and maintain a constant temperature of 60°C during electrophoresis. Time of the run depends on length sizes of the PCR products.
The staining was performed following a fast silver staining protocol.
The gel remains sticked to the plate during the following steps :
- fixation : 30 min. in 10% acetic acid solution,
- 4 baths of 2 min. in deionized water,
- staining : 30 min. in a solution with 0.1% AgNO3, and 0.056 % formaldehyde,
- development in a solution with sodium carbonate 30g/l, sodium thiosulfate 2mg/l, and 0.056 % formaldehyde, until the PCR products are clearly detected,
The reaction is then stopped with chilled 10% acetic acid solution.
The gel can then be scanned and analyzed on an image analysis software.
This technique is essentially intermediate between RFLP and PCR
(Vos et al. 1995). It involves restriction digestion
of the genomic DNA, followed by preamplification and selective amplification
of the restricted fragments. The amplified products are separated on a sequencing
gel and can be visualised after exposure to X-ray film (when radioactive
labels are used).
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- Home
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- Pro-Se Ex-Associate Gets Green Light to Proceed with NY Suit Against UK Law Firm re Moscow Partner
Labor & Employment
Pro-Se Ex-Associate Gets Green Light to Proceed with NY Suit Against UK Law Firm re Moscow Partner
Posted Apr 17, 2012 3:07 PM CDT
By Martha Neil
A fired former associate in the Russia offices of Allen & Overy has been given a green light by a New York judge to sue the major London-based law firm.
Deidre Clark, who made headlines while still working in Moscow for A&O by posting a racy online novel and photos of herself under the pen name Deidre Dare, has prevailed on a motion to dismiss filed by the law firm in state trial court in New York, the Evening Standard reports.
Although one count of Clark's action was nixed, the other seven counts were OK'd to proceed. Clark represented herself in the suit but will soon have attorney Casey Greenfield on her legal team, the newspaper says.
"I won against Allen & Overy! Isn't it amazing?" Clark said after the New York Supreme Court ruling. "You can't imagine how great it feels—pure joy, really. And it's not just a win. I devastated them."
While Clark may have won one round, however, she is still far from prevailing with her overall suit, the law firm points out in a written statement:
"We have always been entirely satisfied that the termination of Ms. Clark's employment was justified and lawful," Allen & Overy told Above the Law. "We remain committed to defending Ms Clark's claim vigorously."
Above the Law provides a link to the 12-page ruling (PDF) on the motion to dismiss earlier this month by Supreme Court Justice Marcy S. Friedman.
While Friedman dismissed a wrongful termination count, she allowed other counts, including sexual harassment, gender discrimination and retaliation to continue because of the at-will nature of Clark's employment. Clark contends that Allen & Overy used the racy novel as a pretext to fire her after a partner in the firm's Moscow office developed an unwelcome sexual interest in her.
Related coverage:
ABAJournal.com: "Allen & Overy Bans American Associate's Racy Online Novel"
ABAJournal.com: "Fired Over Personal Website, Ex-Associate of Major UK Firm Mulls Options"
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Conor Walsh
Swinford, County Mayo
"warm, subtle, evocative tones" (Jim Carrol. Irish Times)
"genuinely spellbinding live'" (James Hendicott, AU Magazine)
"beautiful expressiveness" (Lola Perrin)
"heart-stopping" (Celina Murphy Hotpress magazine)
Minimalist piano/electro acoustic composer from the west of Ireland. Conor has been writing minimalist/ambient pieces for piano since his teens.
His compositions draw upon elements of the traditional, classical,experimental, rock,idm and minimalist genre's . Conor's work is dramatically stark yet soothing, melancholy yet hopeful. Major musical influences include Aphex Twin, Chopin and Tool.
He has made notable appearances at a number of high-profile festivals such as HWCH and the Electric Picnic. Conor's music has been featured in the feature length documentary 'Apples of the Golan', BBC Radio's 'Colum Sands' show and on RTE Radio 1's John Murray Show, RTE Documentary 'Looking out for no. 1' and the Reality Bites series 'Twopair Films' documentary 'Analogue people in a digital age'.
Conor is currently recording an album which will be finished in 2014.
For bookings contact www.conorwalshmusic.com


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The Academy of Realist Art, Boston is an art school that teaches traditional art techniques in drawing classes as well as painting classes, using a curriculum that is modeled on 19th century French academies. Students receive individualized practical instruction advancing at their own pace while working to achieve the highest levels of draftsmanship and painting skills. This intensive and focused teaching methodology has a proven record of success with students enrolling as beginners and those more advanced.
While most of our evening and weekend classes are limited to each semester, you can enroll in Atelier classes at any time. For our printable info brochure, you can download it here.
Fall Semester
Now registering. Semester begins September 2nd.
Sign up early to guarantee your spot in class. We also have a great variety of summer workshops and classes available.


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(via jesuisperdu)
The salty foetid smell of seaweed. The laminated kitchen table, still stained from a cuppa of Liptons, the teabag swollen up like a bloated floater someone had not flushed. Dickhead. Just like a regular day down at Conto's, kids mucking and/or frolicking around in the shallows on their boogie boards and foamy waves staining his dacks (or were they hemp strides?) with white that was very similar in appearance to semen but possibly not. Something about the roundhouse. He had gotten nearly-laid at the park in the middle of the night once, then it had started raining. The rain was probably saliferous. Seagulls shitting on the picnic tables, while nippers threw broken paddle pop sticks bought from the beach kiosk, pretending they were chippies. Gulls explode if you feed them bicarb soda, ya know. The humps of the waves like a preserved soul of a drowned surfer, penis crusted with sand, also the clacker, organ-wrenching glimpses of blood (which is salty) from the great whiteheads crashing spray down again and again and mixing with the familiar scent of the foam and people puking outside Timezone and families frying something up on the beach barbies again. Prawns probably. Someone yelled 'CUNT' down on the limestone street below his window, which was also slightly salty. Or at least had some sand on the windowsill. He sat down on the verandah in his wicker beach chair that was made from brackish driftwood that had washed up on the sand at the Old Maccas near that Freo nautical museum that might still be open. He smiled to himself, running his tongue over his dried lips, his briny ponytail mussed a little by the wind, and nodded. "Booker prize again."
Photo by Adam Cropp
Home of the 'waterspout' spirit.
Location: Raft Point, Kimberley, Western Australia
DONT REPRODUCE wandjina without permission buddy!! check ur priv
(via perthstreetlife)
Some girls wander by mistake/into the mess that scalpels make
Danny Simmons, Confluence of Grievances, 2012. Oil and fabric on canvas, 60 x 48 in
"I was promised I would spend eternity in Paradise, being fed honeyed cakes by 67 virgins in a tree-lined garden, if only I would fly the airplane into one of the Twin Towers," said Mohammed Atta, one of the hijackers of American Airlines Flight 11, between attempts to vomit up the wasps, hornets, and live coals infesting his stomach. "But instead, I am fed the boiling feces of traitors by malicious, laughing Ifrit. Is this to be my reward for destroying the enemies of my faith?"


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Mateo Song and Mixtape Downloads
Mateo - Home for Christmas [Song]
For those who live far away from their loved ones, the holidays are an especially emotional time. If you've got a ticket Home for Christmas, it's a chance to reunite with the people you've been missing all...Read More
Mateo - Sing About Me [Song]
Sing About Me, I'm Dying of Thirst, was arguably the most personal track on Kendrick Lamar's debut studio album, but its central themes of mortality and remembrance have a universal resonance. Interscope/Krucial...Read More
Mateo ft. Stacy Barthe - Looking You Up [Song]
In a musical climate where a R&B star seems to be born every other Tuesday, it really takes true talent to standout among the masses. With the release of Looking You Up, off his latest project Suite 823, it's clear...Read More
Mateo - Carry On [Song]
Did you know that Mateo is the only artist in music history to collaborate with Alicia Keys, Dirty Money and Pusha T? You did? Oh, well then, Carry On. For his latest effort the R&B fast riser rides solo over a Kerry...Read More
Mateo - In Your Arms Tonight [Song]
Last we heard from Mateo, the Krucial Noise singer had reworked Kanye's Power into an R&B cover as fresh as its concept was creative. On newly-leaked mixtape cut In Your Arms Tonight, the artist pulls off a...Read More
Mateo ft. Pusha T & Ab Liva - Power [Song]
By a show of hands, how many of you listened to Kanye's Power for the first time and immediately thought, "This is dope.. but it would be even better as an R&B song!" I obviously can't see you, but...Read More


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A. David Kosoy B.A., J.D. – Chairman & CEO
Well respected figure within the real estate investment community, Mr. David Kosoy has over 40 years of successful real estate investment, lending and development experience throughout Canada, the United States, the United Kingdom, Turks and Caicos Islands and in the Commonwealth of The Bahamas. During his career, Mr. Kosoy has been involved as a principal in more than $1.5 Billion worth of real estate transactions.
Following 15 highly successful years as a founding principal of a Toronto based, privately held, real estate investment company (Brother Developments) Mr. Kosoy directed his attention to the United States where in 1986 he founded the Sterling Companies. The Sterling Companies' original foray into U.S. investments included purchasing more than a dozen assets from the Resolution Trust Company (RTC). Over the next 20 years, The Sterling Companies became widely regarded as one of the preeminent value add shopping center investment and redevelopment companies in each of its selected U.S. markets.
In early 2000, Mr. Kosoy, assumed control of publicly traded Samoth Capital Corp, (nka Sterling Centrecorp Inc.) a mezzanine lending and hotel company on the verge of insolvency. Through acquisition of add-on management and brokerage business and the refocusing of a business plan, Mr. Kosoy, along with partners, took the company from a loss of $45.5 Million in 2000 to profitable years in 2002, 2004, 2005 with an Asset Base of $60.6 Million in 2001 rising to an asset base of over $300 Million in 2005. Mr. Kosoy was instrumental in taking Sterling Centrecorp Inc., publicly listed on the Toronto Stock Exchange, private.
Since 2007, Mr. Kosoy started a mortgage fund, New Providence Capital Management Partners, with funding raised privately. All capital has been deployed to date without any losses to principal or interest at this writing.
Prior to his real estate career, Mr. Kosoy was a partner at the law firms of Sax, Isaacs, Basman and Kosoy, and Kosoy, Gordin and Schiller, both in Toronto, Canada. Mr. Kosoy retired from the practice of Law in 1979 to pursue a real estate related career on a full time basis. Mr. Kosoy has also been very active in philanthropic causes including his chairmanship of State of Israel Bonds (Toronto) and world wide Chairman New Leadership for State of Israel Bonds, The Israel Tennis Center, and on the Board of Governors of the Mount Siani Hospital in Toronto. Mr. Kosoy is a graduate of the University of Akron, Ohio, which he attended on a full football scholarship, and of Osgoode Hall Law School in Toronto, where he made intercollegiate hockey first all-star team. Mr. Kosoy resides in, and is a permanent resident of, the Bahamas.


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Psychonauts 2 not being funded by Minecraft creator
by Steve Watts, shacknews.com, Feb 6, 2013 7:35AM PST
Minecraft creator Markus "Notch" Persson has indicated he is not planning to fund a Psychonauts sequel, saying he doesn't have the time to research such a large investment right now. Read more
PlayStation Store '13 for 13' sale starts tomorrow
by Steve Watts, shacknews.com, Jan 21, 2013 11:00AM PST
Sony is kicking off a sale tomorrow, which includes 50% off several games or 75% off for PlayStation Plus members. Some highlights include Unfinished Swan, LBP Karting, Psychonauts, and Shank 2. Read more
Psychonauts coming to PS3 next week
by Andrew Yoon, shacknews.com, Aug 23, 2012 2:15PM PDT
Psychonauts is getting a PlayStation Network re-release next week, August 28th. Read more
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Remember the days when you would get so excited about the Holiday season rolling around? I sure do. I remember making my Christmas list 2 months early, and then "rearrange" things on the list to update to the newest and hottest items. Don't you guys miss those days? Staying up until the early morning in anticipation of what may be downstairs in the living room awaiting you. And then finally you awake in the morning, go downstairs and do the infamous gasp when you see your new G.I Joes, or other coveted Christmas items for young boys. Man, I cant say it enough, I miss those days more than anything in the world.
I made this post for those of you, who like me, may be looking for your inner young boy. And most importantly, I made this list for our beloved Bobcats to read, and hopefully to make our wishes, a reality.
What you guys do, is make a Holiday list of moves you want made, realistic moves of course, because like your parents probably told you when you were little when you asked for a Ferrari, there is no way in hell you are getting that, just like we would not land Lebron James for Kwame Brown and Dominic McGuire. Make a list of realistic milestones you would like to see the team, or an individual on the team to accomplish.
Well have fun guys, and Happy Holidays :)


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By Lindsey Galloway, Natural Solutions magazine
Europeans have long known the true origin of the word spa: Aanitas per agua, or "health through water." Before facials and massages became synonymous with spas, bathing was the premier avenue to personal wellness and whole-body detoxification. The citizens of ancient Rome considered a daily bath their civic duty to maintain public health. Since the late 1700s and through to the present day, German physicians have prescribed to stressed patients a kur (cure or treatment) consisting of one bath a day for 10 to 20 days. And since the end of the 19th century, the French have indulged in thalassotherapy baths, which harness the anti-aging powers of ocean water and seaweed.
Health Benefits of Epsom Salt Baths
In America, we've just begun to catch on to the skin-saving benefits of relaxing baths, says Andria Roth, lead aesthetician at Oasis Spa in Woodinville, Washington. Recent studies have directly correlated annoying skin problems like acne and eczema with excessive stress–the body releases panic hormones that weaken the skin's defense against breakouts, dry patches, and redness. Thankfully, the ability to reduce that burden on your skin is as close as your bathroom door.
"Our high levels of stress could be mitigated if we just bathed every night," says Ann Brown, director of Spa Shiki in Lake Ozark, Missouri. "It's easy to create a before-bed ritual with the same music and same scent to signal our body that it's time to wind down."
Start your at-home treatment by filling the tub with warm water–about 90 degrees if you want an energizing bath or 105 degrees for a relaxing soak. While the tub's filling, dry brush your skin (toward your heart) for at least five minutes, but shoot for 10 if your skin's not too sensitive. "Body brushing exfoliates the skin and gets the blood right up to the surface," says Brown. "It not only opens up the pores to allow your bath ingredients to penetrate better, but it also helps your skin keep its tone."
Finally, customize the beautifying power of your bath with one or more of the following herbs and oils, and give your skin the relaxation and support it's craving.
For stressed, sensitive skin, add 20 drops of French lavender essential oil or two chamomile tea bags to your water to reduce redness and calm the tension that can lead to breakouts.
For cellulite and puffy skin, take a mineral salts or seaweed bath. Both the salts and the seaweed have negative ions that attract the positively charged ions in skin. Like a magnet, the skin pulls in minerals and nutrients, eventually drawing them all the way into the bloodstream, where they help carry toxins away from the body through the lymph system. Keeping the temperature of the water a little cooler at 94 to 97 degrees will help you to reduce the look of cellulite by stimulating your body to dispose of cellular waste, says Brown.
Next: Baths for dry and tired skin


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- Date:
- Friday January 18, 2013
- Time:
- 6:30pm - 6:00am
- Location:
- Sailor Circus
- Contact Phone:
- 366-0156
- Ticket Price:
- General tickets $175; Patron tickets $250
PHOTO GALLERY: Bird Key Yacht Club Labor Day Celebration
Bird Key Yacht Club kicks off Labor Day weekend with its Labor Day Celebration Party Friday, Aug. 29, at Bird Key Yacht Club.
FYI Friday — Your Weekly news roundup
Catch up on the week's most important and most interesting news stories from Sarasota, Longboat Key and East County with FYI Friday.
Deputies make arrest in murder investigation
The Sarasota County Sheriff's Office has charged 25-year-old Karli Wilbanks with second-degree murder in connection the shooting death of her boyfriend Michael Goodman.
City, county closures scheduled for Labor Day
Government offices in Sarasota County, the city of Sarasota, Longboat Key and East County will close Monday, Sept. 1, in observance of the Labor Day holiday.


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Nothing Up There But Air: If things don't change College Football is going to see a lot more Terrelle Pryors
The Slate loves it when Wednesday is the second day of the work week. Today, the Big 12 meetings will be much calmer this year, Haskin's midweek musings, Carl Torbush retires after cancer...
The Slate is not ready for June. Today, Bat Cats eliminated at Big 12 tournament, but still make NCAA Regional, Jim Tressel resigns at Ohio State, and K-State dedicates WWII monument on campus.
So Long Senator Sweater-Vest - Miami fans have mixed emotions as Jim Tressel resigns before they can get revenge for the 2002 National Championship "robbery".
Jim Tressel has been an enemy for SEC fans for a long time. That doesn't mean we should be happy about his fate.
There are a lot of questions about Jim Tressel's resignation from Ohio State football, and about three answers. We review them, and try to figure out who could be the next head coach of the Buckeyes.
Jim Tressel resigns as Ohio State's head coach. What does it mean for Michigan State?
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