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Welcome to the new Building Connect Content Centre. This new resource centre provides relevant information from industry related companies meant to assist Building's audience in obtaining essential information for their jobs. Here you will find links to mini-sites containing vital information on products, services, and solutions. Whenever you read an article on www.building.co.uk and want to learn more about a particular company's product or services, simply click on that company's name to learn more.
The Building Connect Content Centre brings you information from a wide range of consultants, manufacturers and service providers, when it is most convenient.
Click on a company logo and you will be taken through to view this valuable information
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Singles invited to sing the night away this Valentine's Day (From Stroud News and Journal)
Get involved: send your pictures, video, news and views by texting SNJ NEWS to 80360, or email us
Singles invited to sing the night away this Valentine's Day
10:03am Friday 14th February 2014 in News By Kate Wilson, Reporter
Single this Valentine's Day?
Staff at the Thee Horseshoes pub in Frampton on Severn are hosting the perfect antidote to feeling lonely this February 14 with a karaoke night.
Landlady Jess O'Gorman said: "Every Valentine's Day you are bombarded by reminders that you are single, from meal-deals exclusively for two to mushy couples cards on every supermarket isle.
"I felt the time was right to fight back and give people a chance to say 'I'm single and I can have fun' with an evening dedicated to them"
For more information call the Three Horseshoes pub on 01452 742100 or go to www.threehorseshoespub.co.uk.
Comments are closed on this article.

subset: commoncrawl


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When a record company considers what singles to release from a record, the strongest song on the CD usually leads the way, but occasionally a strong song is held back to help extend the sales of a CD. For OneRepublic, their second CD Waking Up started off with the slow-burning "All the Right Moves," built momentum with "Secrets," and then came their third single "Good Life." Is "Good Life" a good or a great single? Find out in our review of OneRepublic's "Good Life."
Borderless Lyrics
The lyrics of "Good Life" tell the story about how the narrator feels blessed because of the places he's travelled to and the experiences he has encountered. If there is any question that the song is autobiographical for OneRepublic, all one needs to do to be sure is check the lyrics:
"To my friends in New York I say hello
My friends in LA they don't know
Where I've been for the past few years or so
Paris, to China to Colorado"
OneRepublic's origins include both Colorado and Los Angeles, but the song focuses more on where the band has travelled to instead of where they have come from. While the meaning of the song is very clear, the song resonates on both a literal and an interpretive level, since anyone can relate to lyrics like "what the hell is there to complain about" at some point in their lives.
Special Delivery
Not only does "Good Life" have lyrics that anyone can relate to, it also has a unique musical delivery that allows the song to stand out on a radio full of same-sounding songs. Lead songwriter and producer Ryan Tedder has a knack for pulling in musical elements not normally found on a mainstream pop song, and he utilizes that knack by layering in drums that give the song a marching feel combined with a haunting whistle. Add in Ryan Tedder's rapping-singing hybrid on the verses, and you have a record that sounds like nothing else out in the pop world at the moment. It's this uniqueness that helps OneRepublic songs endure for months at pop radio instead of weeks, staying on listeners' minds much longer than they would expect.
What's the Verdict?
OneRepublic released an alternate version of "Good Life" that featured successful rapper B.o.B while the song was already climbing the charts. If the addition of a rap to the song increases the potential audience that gets to experience "Good Life," then it will be doing the listening public a favor. However, for people who have already fallen for "Good Life," the rap may feel a bit unnecessary attached to a pop song many consider great on its own merits. No matter which version someone chooses, they will be hard-pressed to find a pop song more uplifting and joyful than "Good Life."


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The Bradley Basketball team will participate in an exempt tournament for a fifth consecutive year and for the seventh time in Jim Les' nine years as the head coach when the Braves join Massachusetts, Southern California and New Mexico State in the Basketball Hall of Fame Tip-Off Tournament Nov. 20-21 at the MassMutual Center, according to an announcement today by the Naismith Memorial Basketball Hall of Fame.
As part of its participation in the Basketball Hall of Fame Tip-Off Tournament, Bradley will host two games in Peoria against opponents that have yet to be determined. In the games scheduled for Nov. 20 at the MassMutual Center, Bradley will take on USC, while New Mexico State will face UMass. The winners and losers of the Nov. 20 games will meet in the Nov. 21 consolation and championship games. In addition to the four "anchor" schools, Rider will be a fifth tournament participant based on a partnership between the Naismith Memorial Basketball Hall of Fame and the Metro Atlantic Athletic Conference. The complete schedule of campus-site games will be announced at a later date, as well as the remaining three schools, television information and ticket information.
Both Bradley (16-15) and USC (16-14) won 16 games and had multiple victories against Top 20 ranked teams last season. The Braves own a 4-3 lead in the all-time series against the Trojans, but USC has won the last three meetings, including both games of their most-recent home-and-home series in 2000 (107-92) and 2001 (60-50).
Bradley won the only previous meeting against UMass, a 101-73 victory Dec. 28, 1964 at Robertson Memorial Fieldhouse. The Minutemen were 12-20 last season, but did enjoy home wins versus NIT participants Memphis and Rhode Island. Meanwhile, Bradley and New Mexico State were Missouri Valley Conference rivals for 11 seasons from 1972-83 and the Braves own a 13-12 lead in the all-time series. The Aggies are the defending Western Athletic Conference Tournament champions and are coming off a 22-12 campaign that ended with a near upset of eventual Final Four participant Michigan State in the first round of the NCAA Tournament.
Bradley owns a 55-55 all-time record in regular-season tournaments, but the Braves are 16-7 (.696) in six previous in-season tournaments under Les. Included in that record are wins against Miami-Fla. (2003), Rutgers (2006), Iowa (2007) and Illinois (2009).
article courtesy BU Sports Information


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«...Graziano is accused of a firebombing at a Rutherford synagogue and arson at a Paramus synagogue in recent weeks. He is being held on $5 million bail after being charged with a host of crimes including nine counts of attempted murder and the arson and bias intimidation charges.
Authorities say Graziano was responsible for fires at synagogues in Paramus and Rutherford this month. They traced the materials in some of the bombs to a Wal-Mart store and captured surveillance images of a man buying the materials who later was identified as Graziano..».
Note: Graziano — could face a Rocky Road ahead of him ...
Accused synagogue firebomber denies guilt, wants case moved out of Bergen County - NorthJersey.com


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Joanne's save the date
Joanne & Jake wanted a typographical solution for their Save the Dates, a few choice words and a lot of kerning & leading!
[images from Bird Dog Press]
Lindsay's save the dates
Lindsay can't decide which version she prefers….I definitely like the second one best! Anyone else?
[images from Lindsay]
Alicia's save the date
Alicia [from Seaport Flowers, yay!] and her fiance made these adorable save the dates. Gotta love Photoshop!
[images from Alicia]
Carla's save the date
Anyhooo….Dave and I are older (he's 49 I'm almost 42) both of us LOVE LOVE LOVE music. I've had a bunch of Americana bands that I worked with as merch chick, den mother, group therapist, promo guru, photographer etc and Dave IS a musician (with a day job).
Since music is such an important part of both of our lives we made our save the dates cards to look like real concert tickets. Our invitations will look like a Hatch Show Print (a very cool place in Nashville that does amazing work and if we were having a bigger wedding I would have them do the actual invitation but I couldn't justify it for 20-40 invitations when the min order was 100.) The whole wedding weekend is going to be one great big "picking party" since the majority of the guest list are either musician or sing.
[image from Carla]
Leela's save the date
i used to live in brooklyn (le sigh) and am now a soon-to-be bride. I don't know where I found your blog, but it is a refreshing contrast to lots of the wedding broo-ha-ha I seem to sift through . . .I really enjoy your perspective and the items you share . . .I thought you might get a kick out of the save date created by myself and my sweety, david.
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Early morning snow caused traffic problems across Middle Tennessee Saturday morning.
Flurries started to fall in the Metro Nashville area around 6:45 a.m. and, with temperatures in the 20s, the snow quickly coated the roadways.
By 8:30 a.m., cars were scattered all over Interstate 24.
"There were cars everywhere all over the road and up against the retainer wall, people crashing everywhere," Metro police Officer Joe Hooper told Nashville's News 2.
Metro police responded to over 200 accidents. Twenty eight of those accidents involved injuries.
"Have the motorist out here slow it down. Pay attention to their surroundings and keep enough distance between them and the people in front of them," Hooper advised.
By late morning, News 2 meteorologist Davis Nolan said roads were improving.
Warming temperatures were also expected to help keep the roads clear the rest of the day.
Tennessee Department of Transportation spokesperson Deanna Lambert told Nashville's News 2 crews were ahead of the game and had been out all morning working to eliminate slick spots.
Lambert, however, advised motorists to stay home.
"If you don't have to be out on the roads, please don't be on the roads so our trucks can get out there and get through the lanes," she said.
More wintry precipitation is possible Saturday afternoon. East Tennessee can also expect more snowfall.
Check the latest forecast and radar conditions, visits WKRN.com/Weather.
441 Murfreesboro Road Nashville, TN 37210
Telephone: 615.369.7222
Fax: 615.369.7329
Email: firstname.lastname@example.com


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Candy: Little sweet treats you eat.
Candy can be sour, sweet, or tart.
Beküldő: Hidden Meany 2014. július 2.
usually a feline known to show its bits around town...
hey candy i will leave your money on the table, close the door when you let yourself out
Beküldő: jim buck jones 2010. július 19.
A slang term for masterbation, used when small children or other persons are around.
I hear Jennifer makes candy every single day.
Beküldő: Steve Bobo 2010. november 21.
A mixed assortment of rubbers,birth control pills and other contraceptives,jackoff sleeves,LSD,ecstasy,magic mushrooms and marijuana ect. that a band and/or musician toss out to the audiance as a reward for coming.
What kind of candy did you get?
Beküldő: Judge dredd7 2011. július 22.
Extascy, Or a extreme ho that will come home with you after only one or two drinks. Skank, Slut.
Damn, Check that bitch Candy, I didn't even buy her two drinks and she wants to come home so I can put it in her ass. What a slut! I love Candy
Beküldő: Rob Lussier 2006. szeptember 25.


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Woman taken to hospital with neck injury following crash (From Gazette)
Get involved: send your pictures, video, news and views by texting GAZETTE NEWS to 80360, or email
Woman taken to hospital with neck injury following crash in Mersea Road, Colchester
10:50am Monday 14th July 2014 in News
A WOMAN has been taken to hospital with a neck injury following a car crash.
Ambulance crews and police officers were called to Mersea Road, near the Southway roundabout, in Colchester just before 9am this morning after a Vauxhall and Mercedes crashed.
The woman, who is believed to be in her 40s, was taken to Colchester hospital.
Her injures are not thought to be life-threatening.
The accident partially blocked the busy road until about 10.30am.
Comments are closed on this article.


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66 year old Jeannie Baker Lee never thought she'd be sitting telling her story.
Lee says, "I've seen these things on TV before where old people get snookered by some con man or some woman gets married to some jerk who sucks all of her money out of her accounts."
But Lee didn't marry some jerk who drained her dry. The former mortgage underwriter in the real estate industry fell for an investment scheme.
According to court records Ron Lawrence Hamilton claimed he was putting Lee's $110,000 in a new trade platform...A platform that guaranteed she would never lose her money.
"I started to say how is the trade going where is the money what's going on and I got all these stalls and these reasons why not," says Lee.
Finally, after waiting months Lee called in the Harris County Financial crimes unit and they arrested Hamilton.
However, investigators told her Hamilton began spending her money the moment he received it on personal expenses.
Lee says, "Detective Lindsey Williams found a Facebook with Ron Hamilton and the parties looked like Jay Z parties."
In the end Hamilton is facing some serious time behind bars if he's convicted. Meanwhile, Lee says she's lost everything she worked so hard to get including her home.
"I really would like to punish them a little bit but what do you do you would like to get your money back so you can get back to where you were before," says Lee.
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Expired Property Listing
This property is disabled.
Quiet lakeside condo in a gated community in Sarasota
- bedrooms 1
- baths 1
- sleeps 2
- pool yes
- hot tub no
- pets allowed no
- type Condo
- community Villagio
- rates (USD) $ 1,600 - $ 2,400 / month
vacation rental description
Beautifully furnished second floor lakefront unit, with patio facing South, in a fairly new gated community with heated pool.
Extra office cubicle. New king size bed. Hardwood floors throughout the unit.
Granite countertops. New walk-in shower. Covered parking.
Cable TV, Internet WiFi, long-distance (incl. Canada) telephone, water and electricity are included.
Minutes to downtown and the beaches. Next to Ed Smith Stadium - the Orioles' winter training camp.
Bus stop just outside the gate. Club house with fitness room and swimming pool.
A $400 reservation deposit is required to hold the unit. The entire rent amount is due 60 days prior to arrival.
Sorry, no pets and no smoking inside the unit.
Minimum rental period: 1 month.
condo rental amenities
Heated community pool, clubhouse, fitness room, practice putting green, tennis courts.
activities and attractions in Sarasota Gulf Coast Central
Walking distance to Ed Smith stadium.
8 minutes drive to Main Street Sarasota.
12 minutes to St. Armands Circle and the beach.
vacation rental rates
December 2013: $1,800/month.
January 2014: $2,100/month.
February or March 2014: $2,200/month.
April 1st - Nov. 30th, 2014: $1,600/month
$400 reservation deposit to hold the unit.
1 month rental minimum.
Full payment is due 60 days prior to arrival.
A $75 Cleaning Fee and a 12% Florida Tourist Tax required.
A refundable $500 security deposit is due upon arrival.


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Research Topics
Summary: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.
Top Publications
Research Grants
Scientific Experts
Detail Information
Publications334 found, 100 shown here
- Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model systemStefan Pukatzki
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:1528-33. 2006..Using Dictyostelium discoideum as a model host, we have identified a virulence mechanism in a non-O1/non-O139 V...
- dictyBase update 2011: web 2.0 functionality and the initial steps towards a genome portal for the AmoebozoaPascale Gaudet
dictyBase, Northwestern University Biomedical Informatics Center and Center for Genetic Medicine, 420 E Superior St, Chicago, IL 60611, USA
Nucleic Acids Res 39:D620-4. 2011dictyBase (http://www.dictybase.org), the model organism database for Dictyostelium, aims to provide the broad biomedical research community with well integrated, high quality data and tools for Dictyostelium discoideum and related ..
- Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communicationAndrew J Heidel
Leibniz Institute for Age Research Fritz Lipmann Institute, D 07745 Jena, Germany
Genome Res 21:1882-91. 2011b>Dictyostelium discoideum (DD), an extensively studied model organism for cell and developmental biology, belongs to the most derived group 4 of social amoebas, a clade of altruistic multicellular organisms...
- Legionella eukaryotic-like type IV substrates interfere with organelle traffickingKarim Suwwan de Felipe
Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University Medical Center, New York, New York, United States of America
PLoS Pathog 4:e1000117. 2008..of many endosome-like structures containing undigested material when expressed in the protozoan host Dictyostelium discoideum...
- Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarityKristen F Swaney
Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
Annu Rev Biophys 39:265-89. 2010..By exploiting the genetic advantages of Dictyostelium, investigators are working out the complex network of interactions between the proteins that have been ..
- The ordered extension of pseudopodia by amoeboid cells in the absence of external cuesLeonard Bosgraaf
Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
PLoS ONE 4:e5253. 2009..movement by analyzing the direction, size and timing of approximately 2000 pseudopodia that are extended by Dictyostelium cells...
- Conserved developmental transcriptomes in evolutionarily divergent speciesAnup Parikh
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Genome Biol 11:R35. 2010..The social amoebae Dictyostelium discoideum and Dictyostelium purpureum have similar developmental morphologies although their genomes are as ..
- Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureumRichard Sucgang
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Genome Biol 12:R20. 2011..There are four groups of dictyostelids, with the most derived being a group that contains the model species Dictyostelium discoideum.
- Transient localized patterns in noise-driven reaction-diffusion systemsInbal Hecht
Center for Theoretical Biological Physics, University of California San Diego, La Jolla, California 92093 0319, USA
Phys Rev Lett 104:158301. 2010..Our work is motivated by a recent biological example showing transiently excited cell membrane regions...
- Attenuation of phospholipid signaling provides a novel mechanism for the action of valproic acidXuehua Xu
School of Biological Sciences, Royal Holloway University of London, Egham, United Kingdom
Eukaryot Cell 6:899-906. 2007..Using the social amoeba Dictyostelium discoideum, we show that an acute effect of VPA is the inhibition of chemotactic cell movement, a process ..
- Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoaChristopher T Price
Department of Microbiology and Immunology, College of Medicine, University of Louisville, Kentucky, USA
PLoS Pathog 5:e1000704. 2009..rapid acquisition of polyubiquitinated proteins by the Legionella-containing vacuole (LCV) within macrophages, Dictyostelium discoideum, and Acanthamoeba...
- Chemotaxis in the absence of PIP3 gradientsOliver Hoeller
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
Curr Biol 17:813-7. 2007Chemotaxing neutrophils and Dictyostelium amoebae produce in their plasma membranes the signaling lipid PI(3,4,5)P3 (PIP3) in gradients, which are orientated with the external chemotactic gradient and have been proposed to act as an ..
- Spatio-temporal analysis of eukaryotic cell motility by improved force cytometryJuan C Del Alamo
Department of Mechanical and Aerospace Engineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 104:13343-8. 2007..method and apply it to the analysis of the dynamics of the chemotactic migration of the amoeboid form of Dictyostelium discoideum...
- External and internal constraints on eukaryotic chemotaxisDanny Fuller
Cell and Developmental Biology, Division of Biological Sciences, Department of Physics, and Center for Theoretical Biological Physics, University of California, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 107:9656-9. 2010..Here we determine the chemotactic response of Dictyostelium cells to exponential gradients of varying steepness and local concentration of the chemoattractant cAMP...
- Tumor suppressor PTEN mediates sensing of chemoattractant gradientsMiho Iijima
Department of Cell Biology, Johns Hopkins University, School of Medicine, 21205, Baltimore, MD, USA
Cell 109:599-610. 2002..Disruption of the PI 3-phosphatase, PTEN, in Dictyostelium discoideum dramatically prolonged and broadened the PH domain relocation and actin polymerization responses, ..
- Persistent cell motion in the absence of external signals: a search strategy for eukaryotic cellsLiang Li
Department of Physics, Princeton University, Princeton, New Jersey, United States of America
PLoS ONE 3:e2093. 2008..cells have a search strategy when they are beyond the range of the signal they would otherwise move toward? Here we ask if single, isolated, Dictyostelium and Polysphondylium amoebae bias their motion in the absence of external cues.
- Cells navigate with a local-excitation, global-inhibition-biased excitable networkYuan Xiong
Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
Proc Natl Acad Sci U S A 107:17079-86. 2010..Our study brings together heretofore diverse observations on spontaneous cytoskeletal activity, signaling responses to temporal stimuli, and spatial gradient sensing into a unified scheme...
- Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusionsNatalie Andrew
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
Nat Cell Biol 9:193-200. 2007..multiple cell types are usually generated when existing ones bifurcate and are rarely made de novo; second, in Dictyostelium cells in shallow chemoattractant gradients, pseudopods are made at the same rate whether cells are moving up ..
- Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hostsMonica Hagedorn
Departement de Biochimie, Faculte des Sciences, Universite de Geneve, Sciences II, 30 Quai Ernest Ansermet, CH 1211 Geneve 4, Switzerland
Science 323:1729-33. 2009..Using the social amoeba Dictyostelium as a genetically tractable host for pathogenic mycobacteria, we discovered that M. tuberculosis and M...
- Single-gene greenbeard effects in the social amoeba Dictyostelium discoideumDavid C Queller
Department of Ecology and Evolution, MS 170, Rice University, Post Office Box 1892, Houston, TX 77251 1892, USA
Science 299:105-6. 2003..The csA gene in Dictyostelium discoideum acts as a single-gene greenbeard...
- Dictyostelium finds new roles to modelJeffrey G Williams
College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
Genetics 185:717-26. 2010..made using the model be of wider relevance? This review addresses these issues for the social amoeba Dictyostelium and highlights some of the organisms more recent applications...
- The neuroprotective agent, valproic acid, regulates the mitogen-activated protein kinase pathway through modulation of protein kinase A signalling in Dictyostelium discoideumKatrina Boeckeler
Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK
Eur J Cell Biol 85:1047-57. 2006..To investigate the molecular basis of this effect, we have used the biomedical model system Dictyostelium discoideum to dissect this signalling pathway...
- Phosphoinositides differentially regulate bacterial uptake and Nramp1-induced resistance to Legionella infection in DictyosteliumBarbara Peracino
Department of Clinical and Biological Sciences, University of Turin, AOU S Luigi, Reg Gonzole 10, 10043 Orbassano, Torino, Italy
J Cell Sci 123:4039-51. 2010..of PI3K, PTEN or PLC on Escherichia coli and Legionella pneumophila uptake by the professional phagocyte Dictyostelium discoideum. We show that L...
- Dictyostelium discoideum chemotaxis: threshold for directed motionLoling Song
Laboratory of Atomic and Solid State Physics, Department of Physics, Cornell University, Ithaca, NY 14853, USA
Eur J Cell Biol 85:981-9. 2006The chemotactic response of Dictyostelium discoideum cells to stationary, linear gradients of cyclic adenosine 3',5'-monophosphate (cAMP) was studied using microfluidic devices...
- Genetic control of lithium sensitivity and regulation of inositol biosynthetic genesJason King
School of Biosciences, Cardiff University, Cardiff, UK
PLoS ONE 5:e11151. 2010..An alternative approach is to investigate the genetics of Li(+) sensitivity. Using the social amoeba Dictyostelium, we previously identified prolyl oligopeptidase (PO) as a modulator of Li(+) sensitivity...
- Adenylyl cyclase localization regulates streaming during chemotaxisPaul W Kriebel
Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Building 37 Room 1E24, Bethesda, MD 20892, USA
Cell 112:549-60. 2003We studied the role of the adenylyl cyclase ACA in Dictyostelium discoideum chemotaxis and streaming. In this process, cells orient themselves in a head to tail fashion as they are migrating to form aggregates...
- An autoregulatory circuit for long-range self-organization in Dictyostelium cell populationsSatoshi Sawai
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Nature 433:323-6. 2005Nutrient-deprived Dictyostelium amoebae aggregate to form a multicellular structure by chemotaxis, moving towards propagating waves of cyclic AMP that are relayed from cell to cell...
- The cyclase-associated protein CAP as regulator of cell polarity and cAMP signaling in DictyosteliumAngelika A Noegel
Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany
Mol Biol Cell 15:934-45. 2004..We show that cell polarization, F-actin organization, and phototaxis are altered in a Dictyostelium CAP knockout mutant...
- Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACGElisa Alvarez-Curto
School of Life Sciences, University of Dundee, Dundee, Scotland, UK
Biochem J 401:309-16. 2007..crucial roles in controlling cell movement and gene regulation throughout development of the social amoeba Dictyostelium discoideum...
- Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideumSarah T Miyata
Department of Medical Microbiology and Immunology, 1 63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Infect Immun 79:2941-9. 2011..We present VasX as a novel virulence factor of the T6SS, as a V. cholerae mutant lacking vasX exhibits a phenotype of attenuated virulence toward Dictyostelium discoideum.
- Quantifying in vivo phosphoinositide turnover in chemotactically competent Dictyostelium cellsNadine Pawolleck
Bourne Laboratory, Biomedical Sciences Division, School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK
Methods Mol Biol 571:283-90. 2009Phosphoinositide (PI) signalling is one of multiple signalling cascades involved in chemotaxis in Dictyostelium discoideum...
- Loss of Dictyostelium ATG9 results in a pleiotropic phenotype affecting growth, development, phagocytosis and clearance and replication of Legionella pneumophilaSze Man Tung
Zentrum für Biochemie, Universitat zu Koln, Germany
Cell Microbiol 12:765-80. 2010Infection of Dictyostelium discoideum with Legionella pneumophila resulted in a large number of differentially regulated genes among them three core autophagy genes, ATG8, ATG9 and ATG16...
- Chemotaxis: finding the way forward with DictyosteliumJason S King
CRUK Beatson Institute, Bearsden, Glasgow, UK
Trends Cell Biol 19:523-30. 2009..This has led to the popularity of Dictyostelium discoideum, with its experimentally-friendly lifestyle and small, haploid genome, as a tool to dissect the ..
- Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideumGrant P Otto
Department of Anatomy and Cell Biology, Columbia University, 630 West 168th St, New York, NY 10032, USA
Mol Microbiol 51:63-72. 2004..pneumophila infection requires macroautophagy. As L. pneumophila replicates in Dictyostelium discoideum, and macroautophagy genes have been identified and mutated in D...
- Autophosphorylation activates Dictyostelium myosin II heavy chain kinase A by providing a ligand for an allosteric binding site in the alpha-kinase domainScott W Crawley
Department of Biochemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
J Biol Chem 286:2607-16. 2011b>Dictyostelium discoideum myosin II heavy chain kinase A (MHCK A), a member of the atypical α-kinase family, phosphorylates sites in the myosin II tail that block filament assembly...
- WASP family proteins: their evolution and its physiological implicationsDouwe M Veltman
Beatson Institute for Cancer Research, Glasgow, United Kingdom
Mol Biol Cell 21:2880-93. 2010..An unusual Dictyostelium WASP homologue that has lost the WH1 domain has retained its function in clathrin-mediated endocytosis, ..
- Orientation of chemotactic cells and growth cones: models and mechanismsH Meinhardt
Max Planck Institut fur Entwicklungsbiologie, Spemannstr 35, D 72076 Tubingen, Germany
J Cell Sci 112:2867-74. 1999..Travelling waves or oscillations in counter phase can emerge on the cell cortex, in agreement with observations reported in the literature. In other ranges of parameters, the model accounts for the generation of a stable cell polarity...
- Two complementary, local excitation, global inhibition mechanisms acting in parallel can explain the chemoattractant-induced regulation of PI(3,4,5)P3 response in dictyostelium cellsLan Ma
Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
Biophys J 87:3764-74. 2004Chemotaxing cells, such as Dictyostelium and mammalian neutrophils, sense shallow chemoattractant gradients and respond with highly polarized changes in cell morphology and motility...
- Exploring the control circuit of cell migration by mathematical modelingJavier Satulovsky
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Biophys J 94:3671-83. 2008..we show that the mechanism of local stimulation global inhibition can readily account for the behavior of Dictyostelium under a large collection of conditions...
- Navigation of chemotactic cells by parallel signaling to pseudopod persistence and orientationLeonard Bosgraaf
Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
PLoS ONE 4:e6842. 2009..Here we use a new computational algorithm to analyze the extension of pseudopods by Dictyostelium cells...
- Collective cell migration in developmentCornelis J Weijer
Division of Cell and Developmental Biology, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
J Cell Sci 122:3215-23. 2009....
- Towards a molecular understanding of human diseases using Dictyostelium discoideumRobin S B Williams
Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London, WC1E 6BT, UK
Trends Mol Med 12:415-24. 2006The social amoeba Dictyostelium discoideum is increasingly being used as a simple model for the investigation of problems that are relevant to human health...
- Self-organization of the phosphatidylinositol lipids signaling system for random cell migrationYoshiyuki Arai
Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
Proc Natl Acad Sci U S A 107:12399-404. 2010..waves of PtdIns 3,4,5-trisphosphate [PtdIns(3,4,5)P(3)] are generated spontaneously on the membrane of Dictyostelium cells in the absence of a chemoattractant...
- The mood stabiliser lithium suppresses PIP3 signalling in Dictyostelium and human cellsJason S King
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, UK
Dis Model Mech 2:306-12. 2009..By using the genetically tractable organism Dictyostelium, we show that lithium suppresses PIP(3)-mediated signalling...
- The novel tyrosine kinase ZAK1 activates GSK3 to direct cell fate specificationL Kim
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell 99:399-408. 1999..Inhibition of GSK3 by 7-TM Wnt/wg receptor signaling is critical for specifying embryonic cell fate patterns. In Dictyostelium, the 7-TM cAMP receptors regulate GSK3 by parallel, antagonistic pathways to establish a developmental body ..
- Ordered patterns of cell shape and orientational correlation during spontaneous cell migrationYusuke T Maeda
Department of Physics, Graduate School of Science, The University of Tokyo, Tokyo, Japan
PLoS ONE 3:e3734. 2008..Yet little is known about how cells change their own shape and how cells coordinate the deformation and movement. Here, we investigated the mechanism of spontaneous cell migration by using computational analyses...
- How human leukocytes track down and destroy pathogens: lessons learned from the model organism Dictyostelium discoideumTian Jin
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
Immunol Res 43:118-27. 2009..Interestingly, a soil amoeba, Dictyostelium discoideum, uses these same behaviors to pursue and injest its bacterial food source and to organize its multi-..
- Functional analysis of spontaneous cell movement under different physiological conditionsHiroaki Takagi
Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
PLoS ONE 3:e2648. 2008..For that purpose, we observed a series of spontaneous movements by Dictyostelium cells at different developmental periods by using a single cell tracking system...
- Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideumGrant P Otto
Department of Anatomy and Cell Biology, Columbia University, New York, New York 10032, USA
J Biol Chem 278:17636-45. 2003..In Dictyostelium, development is induced by starvation, conditions under which autophagy is required for survival in yeast and ..
- An Elmo-like protein associated with myosin II restricts spurious F-actin events to coordinate phagocytosis and chemotaxisNilgun Isik
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
Dev Cell 15:590-602. 2008..We identified an Elmo-like protein, ElmoA, in Dictyostelium discoideum that unexpectedly functions as a negative regulator of actin polymerization...
- Dictyostelium Aurora kinase has properties of both Aurora A and Aurora B kinasesHui Li
Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
Eukaryot Cell 7:894-905. 2008..We show that Dictyostelium discoideum contains a single Aurora kinase, DdAurora, that displays characteristics of both Aurora A and B...
- Overlapping functions of the two talin homologues in DictyosteliumMasatsune Tsujioka
National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
Eukaryot Cell 7:906-16. 2008..The cellular slime mold Dictyostelium discoideum has two talin homologues, talA and talB, and earlier studies have characterized the single knockout ..
- Propagating chemoattractant waves coordinate periodic cell movement in Dictyostelium slugsD Dormann
School of Life Sciences, Division of Cell and Developmental Biology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
Development 128:4535-43. 2001Migration and behaviour of Dictyostelium slugs results from coordinated movement of its constituent cells. It has been proposed that cell movement is controlled by propagating waves of cAMP as during aggregation and in the mound...
- Regulation of PTEN by Rho small GTPasesZhong Li
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Nat Cell Biol 7:399-404. 2005..PTEN's intracellular localization and presumably activity are regulated by chemoattractants in Dictyostelium and mouse neutrophils. However, the mechanisms for its regulation remain elusive...
- Gene discovery in the Acanthamoeba castellanii genomeIain J Anderson
Department of Parasite Genomics, The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, MD 20850, USA
Protist 156:203-14. 2005..The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A...
- Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKaC Y Chung
Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0634, USA
Mol Cell 7:937-47. 2001..Addition of the PI3K inhibitor LY294002 results in a rapid loss of cell polarity and the axial distribution of actin, myosin, and PAKa. These results provide a mechanism by which PI3K regulates chemotaxis...
- A Rap/phosphatidylinositol 3-kinase pathway controls pseudopod formation [corrected]Arjan Kortholt
Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, The Netherlands
Mol Biol Cell 21:936-45. 2010GbpD, a Dictyostelium discoideum guanine exchange factor specific for Rap1, has been implicated in adhesion, cell polarity, and chemotaxis...
- A common mechanism of action for three mood-stabilizing drugsRobin S B Williams
Intracellular Signalling Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower St, London WC1E 6BT, UK
Nature 417:292-5. 2002..Moreover, the development of Dictyostelium is sensitive to lithium and to valproic acid, but resistance to both is conferred by deletion of the gene that ..
- Primitive agriculture in a social amoebaDebra A Brock
Department of Ecology and Evolutionary Biology, Rice University, 6100 Main Street, Houston, Texas 77005, USA
Nature 469:393-6. 2011..Here we show that the social amoeba Dictyostelium discoideum has a primitive farming symbiosis that includes dispersal and prudent harvesting of the crop...
- Automated characterization of cell shape changes during amoeboid motility by skeletonizationYuan Xiong
Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
BMC Syst Biol 4:33. 2010....
- The antiepileptic drug valproic acid and other medium-chain fatty acids acutely reduce phosphoinositide levels independently of inositol in DictyosteliumPishan Chang
Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham, TW20 0EX, UK
Dis Model Mech 5:115-24. 2012..work identified a previously unknown effect of VPA in reducing phosphoinositide production in the simple model Dictyostelium followed by the transfer of data to a mammalian synaptic release model...
- C-sequence of the Dictyostelium cytoplasmic dynein participates in processivity modulationNaoki Numata
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3 8 1 Komaba, Meguro ku, Tokyo, Japan
FEBS Lett 585:1185-90. 2011We examined the functional roles of C-sequence, a 47-kDa non-AAA+ module at the C-terminal end of the 380-kDa Dictyostelium dynein motor domain...
- MidA is a putative methyltransferase that is required for mitochondrial complex I functionSergio Carilla-Latorre
Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, 28029 Madrid, Spain
J Cell Sci 123:1674-83. 2010b>Dictyostelium and human MidA are homologous proteins that belong to a family of proteins of unknown function called DUF185...
- The in vivo role of annexin VII (synexin): characterization of an annexin VII-deficient Dictyostelium mutant indicates an involvement in Ca(2+)-regulated processesV Doring
Max Planck Institut fur Biochemie, Martinsried, FRG
J Cell Sci 108:2065-76. 1995b>Dictyostelium discoideum cells harbor two annexin VII isoforms of 47 and 51 kDa which are present throughout development...
- Coupling mechanism of a GPCR and a heterotrimeric G protein during chemoattractant gradient sensing in DictyosteliumXuehua Xu
Chemotaxis Signal Section, National Institutes of Health, Rockville, MD 20852, USA
Sci Signal 3:ra71. 2010..We suggest that chemoattractant receptors use a ligand-induced coupling rather than a precoupled mechanism to control the activation of G proteins during chemotaxis...
- Use of a Dictyostelium model for isolation of genetic loci associated with phagocytosis and virulence in Klebsiella pneumoniaeYi Jiun Pan
Department of Microbiology, National Taiwan University College of Medicine, 1, Sec 1, Jen Ai Rd, Taipei 10051, Taiwan
Infect Immun 79:997-1006. 2011Phagocytosis resistance is an important virulence factor in Klebsiella pneumoniae. Dictyostelium has been used to study the interaction between phagocytes and bacteria because of its similarity to mammalian macrophages...
- Proteasomes from Dictyostelium discoideum: characterization of structure and functionT M Schauer
Max Planck Institute for Biochemistry, Martinsried, Germany
J Struct Biol 111:135-47. 1993We have isolated and purified 20 S proteasomes from Dictyostelium discoideum and characterized their proteolytic activities. Two-dimensional electrophoresis revealed 13 distinct spots...
- SCAR knockouts in Dictyostelium: WASP assumes SCAR's position and upstream regulators in pseudopodsDouwe M Veltman
Beatson Institute for Cancer Research, Glasgow G61 1BD, Scotland, UK
J Cell Biol 198:501-8. 2012..We show that, unexpectedly, Dictyostelium discoideum SCAR knockouts could still spread, migrate, and chemotax using pseudopods driven by the Arp2/3 ..
- Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxisHidekazu Kuwayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
PLoS ONE 4:e6658. 2009In the early stages of development of the cellular slime mold Dictyostelium discoideum, chemotaxis toward cAMP plays a pivotal role in organizing discrete cells into a multicellular structure...
- Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplificationArjan Kortholt
Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
EMBO Rep 12:1273-9. 2011..We used Dictyostelium mutants to investigate the minimal requirements for chemotaxis, and identified a basal signalling module ..
- The group migration of Dictyostelium cells is regulated by extracellular chemoattractant degradationGene L Garcia
Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 20:3295-304. 2009Starvation of Dictyostelium induces a developmental program in which cells form an aggregate that eventually differentiates into a multicellular structure...
- Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideumD Warnecke
Universitat Hamburg, Institut fur Allgemeine Botanik, 22609 Hamburg, Germany
J Biol Chem 274:13048-59. 1999..4.1.173). We cloned genes from three different yeasts and from Dictyostelium discoideum, the deduced amino acid sequences of which all showed similarities with plant sterol ..
- A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxisJianshe Yan
Chemotaxis Signal Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
Dev Cell 22:92-103. 2012..Here, we identified a Gβγ effector, ElmoE in Dictyostelium, and demonstrated that it is required for GPCR-mediated chemotaxis...
- A role for tRNA(His) guanylyltransferase (Thg1)-like proteins from Dictyostelium discoideum in mitochondrial 5'-tRNA editingMaria G Abad
Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
RNA 17:613-23. 2011..Here we investigate the activities of four Thg1-like proteins (TLPs) encoded by the genome of the slime mold, Dictyostelium discoideum (a member of the eukaryotic supergroup Amoebozoa)...
- Formation of Hirano bodies in Dictyostelium and mammalian cells induced by expression of a modified form of an actin-crosslinking proteinAndrew G Maselli
Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
J Cell Sci 115:1939-49. 2002..activated actin binding and calcium-insensitive actin filament crosslinking activity were expressed in Dictyostelium and mammalian cells to assess the behavior of these modified forms in vivo...
- Functional plasticity of CH domainsMario Gimona
Department of Cell Biology, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria
FEBS Lett 513:98-106. 2002....
- Identifying an uptake mechanism for the antiepileptic and bipolar disorder treatment valproic acid using the simple biomedical model DictyosteliumNicole Terbach
Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham TW200EX, UK
J Cell Sci 124:2267-76. 2011..of cellular, molecular and genetic approaches to characterize VPA uptake using a simple biomedical model, Dictyostelium discoideum. We show that VPA is taken up against an electrochemical gradient in a dose-dependent manner...
- Xpf and not the Fanconi anaemia proteins or Rev3 accounts for the extreme resistance to cisplatin in Dictyostelium discoideumXiao Yin Zhang
Medical Research Council, Laboratory for Molecular Biology, Cambridge, United Kingdom
PLoS Genet 5:e1000645. 2009Organisms like Dictyostelium discoideum, often referred to as DNA damage "extremophiles", can survive exposure to extremely high doses of radiation and DNA crosslinking agents...
- Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopiesYoshiaki Iwadate
Department of Functional Molecular Biology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan
Biotechniques 44:739-50. 2008..detection of the spatiotemporal distribution of traction forces produced by individual filopodia in migrating Dictyostelium cells, as well as simultaneous visualization of these traction forces and the dynamics of filamentous myosin II...
- Changing directions in the study of chemotaxisRobert R Kay
MRC Laboratory of Molecular Biology, Hill Road, Cambridge CB2 0QH, UK
Nat Rev Mol Cell Biol 9:455-63. 2008....
- Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cellsMasamitsu Kanada
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
Mol Biol Cell 16:3865-72. 2005..Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces...
- Myosin II-independent cytokinesis in Dictyostelium: its mechanism and implicationsT Q Uyeda
Biomolecular Research Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki, Japan
Cell Struct Funct 25:1-10. 2000Similar to higher animal cells, ameba cells of the cellular slime mold Dictyostelium discoideum form contractile rings containing filaments of myosin II during mitosis, and it is generally believed that contraction of these rings bisects ..
- The role of cell contraction and adhesion in dictyostelium motilityMathias Buenemann
Center for Theoretical Biological Physics, University of California, San Diego, La Jolla, California, USA
Biophys J 99:50-8. 2010The crawling motion of Dictyostelium discoideum on substrata involves a number of coordinated events including cell contractions and cell protrusions...
- Glycogen synthase kinase 3 regulates cell fate in DictyosteliumA J Harwood
Medical Research Council, Laboratory of Molecular Biology, Cambridge, England
Cell 80:139-48. 1995Extracellular cyclic AMP (cAMP) induces the formation of prespore cells in Dictyostelium but inhibits stalk cell formation...
- Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signalingPaul B Bokko
Department of Microbiology, La Trobe University, Melbourne, Victoria 3086, Australia
Mol Biol Cell 18:1874-86. 2007..a pharmacological AMPK activator in mammalian cells, mimics mitochondrial disease in impairing Dictyostelium phototaxis and that AMPKalpha antisense-inhibited cells were resistant to this effect...
- Receptor-dependent and tyrosine phosphatase-mediated inhibition of GSK3 regulates cell fate choiceLeung Kim
Laboratory of Cellular and Developmental Biology, Building 50 3351, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
Dev Cell 3:523-32. 2002Asymmetric body axis formation is central to metazoan development. Dictyostelium establishes an anterior/posterior axis utilizing seven-transmembrane cAMP morphogen receptors (CARs) and GSK3-mediated signal transductions that has a ..
- Dictyostelium myosin-IE is a fast molecular motor involved in phagocytosisUlrike Dürrwang
Abteilung Biophysik, Max Planck Institut fur medizinische Forschung, Jahnstr 29, 69120 Heidelberg, Germany
J Cell Sci 119:550-8. 2006..A complete analysis of the kinetic and motor properties of Dictyostelium discoideum myosin-IE was achieved by the use of motor domain constructs with artificial lever arms...
- Cell speed, persistence and information transmission during signal relay and collective migrationColin P McCann
Department of Physics, University of Maryland College Park, College Park, MD 20742 4111, USA
J Cell Sci 123:1724-31. 2010Collective migration is a key feature of the social amoebae Dictyostelium discoideum, where the binding of chemoattractants leads to the production and secretion of additional chemoattractant and the relay of the signal to neighboring ..
- Directional sensing in eukaryotic chemotaxis: a balanced inactivation modelHerbert Levine
Center for Theoretical Biological Physics, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 103:9761-6. 2006Many eukaryotic cells, including Dictyostelium discoideum amoebae, fibroblasts, and neutrophils, are able to respond to chemoattractant gradients with high sensitivity...
- Myosin II is essential for the spatiotemporal organization of traction forces during cell motilityRuedi Meili
Section of Cell and Developmental Biology, Division of Biological Sciences, Department of Mechanical and Aerospace Engineering, and Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
Mol Biol Cell 21:405-17. 2010..However, it does not reduce significantly the magnitude of the traction forces, uncovering a non-MyoII-mediated mechanism for the contractility of the cell...
- General trends in trace element utilization revealed by comparative genomic analyses of Co, Cu, Mo, Ni, and SeYan Zhang
Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 285:3393-405. 2010..examples of increased utilization of proteins that use copper in land plants, cobalt in Dehalococcoides and Dictyostelium, and selenium in fish and algae, whereas nematodes were found to have great diversity of copper transporters...
- Transcriptional regulation of Dictyostelium pattern formationJeffrey G Williams
School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
EMBO Rep 7:694-8. 2006On starvation, Dictyostelium cells form a terminally differentiated structure, known as the fruiting body, which comprises stalk and spore cells...
- Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patchesMarten Postma
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Cell Sci 117:2925-35. 2004The leading edge of Dictyostelium cells in chemoattractant gradients can be visualized using green fluorescent protein (GFP) tagged to the pleckstrin-homology (PH) domain of cytosolic regulator of adenylyl cyclase (CRAC), which ..
- Chemotaxis, chemokine receptors and human diseaseTian Jin
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12441 Parklawn Drive, Rockville, MD 20852, USA
Cytokine 44:1-8. 2008..In this review, we wish to mention landmark discoveries in the chemotaxis research field that shaped our current understanding of this fundamental cell behavior and lay out key questions that remain to be addressed in the future...
- Functional characterization of intracellular Dictyostelium discoideum P2X receptorsMelanie J Ludlow
Department of Cell Physiology and Pharmacology, University of Leicester, Leicester LE1 9HN, United Kingdom
J Biol Chem 284:35227-39. 2009..ion channel activation, extracellular ATP evokes a rapid and transient calcium influx in the model eukaryote Dictyostelium discoideum. Five P2X-like proteins (dP2XA-E) are present in this organism...
- Dictyostelium discoideum--a model for many reasonsSarah J Annesley
Department of Microbiology, La Trobe University, Bundoora, VIC 3086, Australia
Mol Cell Biochem 329:73-91. 2009The social amoeba or cellular slime mould Dictyostelium discoideum is a "professional" phagocyte that has long been recognized for its value as a biomedical model organism, particularly in studying the actomyosin cytoskeleton and ..
- Eat, kill or die: when amoeba meets bacteriaPierre Cosson
University of Geneva, Faculty of Medicine, Centre Medical Universitaire, Dpt de Physiologie Cellulaire et Métabolisme, 1 rue Michel Servet, CH1211 Geneva 4, Switzerland
Curr Opin Microbiol 11:271-6. 2008..b>Dictyostelium discoideum is by far the most studied amoeba, and it is proving useful to analyze phagocytosis and ..
- Dictyostelium discoideum as a model system for identification of Burkholderia pseudomallei virulence factorsBenjamin M Hasselbring
Department of Microbiology, 210 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
Infect Immun 79:2079-88. 2011..the closely related nearly avirulent species Burkholderia thailandensis to predation by the phagocytic amoeba Dictyostelium discoideum...
- A coat of filamentous actin prevents clustering of late-endosomal vacuoles in vivoAnja Drengk
Abteilung Zellbiologie and Center for Interdisciplinary Nanostructure Science and Technology CINSaT, Universitat Kassel, Heinrich Plett Str 40, 34132, Kassel, Germany
Curr Biol 13:1814-9. 2003..Here, we investigate the function of the actin coat that surrounds late endosomes in Dictyostelium. Latrunculin treatment leads to aggregation of these endosomes into grape-like clusters and completely blocks ..
- Infection in a dish: high-throughput analyses of bacterial pathogenesisC Leopold Kurz
Centre d immunologie de Marseille Luminy, Universite de la Mediterranee, Case 906, 13288 Marseille Cedex 9, France
Curr Opin Microbiol 10:10-6. 2007Diverse aspects of host-pathogen interactions have been studied using non-mammalian hosts such as Dictyostelium discoideum, Caenorhabditis elegans, Drosophila melanogaster and Danio rerio for more than 20 years...
- Cross-induction of cell types in Dictyostelium: evidence that DIF-1 is made by prespore cellsR R Kay
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
Development 128:4959-66. 2001To investigate how cell type proportions are regulated during Dictyostelium development, we have attempted to find out which cell type produces DIF-1, a diffusible signal molecule inducing the differentiation of prestalk-O cells...
Research Grants88
- Cellular and Biochemical Studies of Myosin Assembly in DictyosteliumThomas Egelhoff; Fiscal Year: 2009..We are using the simple amoeba Dictyostelium discoideum as a model system for identifying signaling pathways that regulate myosin assembly...
- Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1Diane L Barber; Fiscal Year: 2010..We showed a leading-edge H+ efflux by NHE1 in mammalian fibroblasts and in Dictyostelium cells is necessary for three stages in cell migration: polarity, actin filament assembly driving membrane ..
- Quantitative Analysis of Chemotactic Motility Cycle of Ameboid CellsJUAN CARLOS contact LASHERAS; Fiscal Year: 2010..b>Dictyostelium discoideum has proven to be an excellent model for the chemotactic migration of amoeboid cells such as ..
- CYTOPLASMIC FUCOSYLATIONChristopher West; Fiscal Year: 1993..migration, spore coat formation, spore germination, and protein transport rate, in the cellular slime mold Dictyostelium discoideum...
- INTRACELLULAR ROLE OF CLATHRIN AND ITS REGULATIONTHERESA O HALLORAN; Fiscal Year: 2007..Our experimental strategy is to use Dictyostelium as a model system...
- MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATIONJames Spudich; Fiscal Year: 1999..Our demonstrations that we can use Dictyostelium to express large amounts of functional myosin and soluble fragments that contain the motor domain allow us to ..
- Actions of the Sodium-Hydrogen Exchanger Subtype, NHE1DIANE BARBER; Fiscal Year: 2007..A novel DNHE1 in Drosophila that binds an ERM ortholog, and a novel DdNHE1 in Dictyostelium discoideum that is necessary for cell polarity and chemotaxis were identified, suggesting that actin anchoring ..
- ROLE OF MDR-RELATED GENES IN DICTYOSTELIUM DEVELOPMENTAdam Kuspa; Fiscal Year: 1999..While the development of Dictyostelium is simpler than that of metazoans, resulting in a ball of spores held on a cellular stalk, proportioning of ..
- Spatial regulation of cytoskeleton during chemotaxisChang Y Chung; Fiscal Year: 2010..is necessary for many biological functions, including wound healing, axonal guidance, and the aggregation of Dictyostelium cells. Chemotaxis also plays a role in disease states such as arthritis, cancer, and multiple sclerosis...
- Spatial regulation of cytoskeleton during chemotaxisChang Y Chung; Fiscal Year: 2010..is necessary for many biological functions, including wound healing, axonal guidance, and the aggregation of Dictyostelium cells. Chemotaxis also plays a role in disease states such as arthritis, cancer, and multiple sclerosis...
- SEQUENCING THE DICTYOSTELIUM GENOMEAdam Kuspa; Fiscal Year: 2004..b>Dictyostelium is a social amoeba whose study has contributed to our understanding of diverse biological processes common to ..
- MESSENGER RNA METABOLISM IN DICTYOSTELIUM AND YEASTAllan Jacobson; Fiscal Year: 1992..Therefore, we propose to address the possibility that specific elements (sequences or structures) of unstable Dictyostelium and yeast mRNAs are responsible for the instability of those mRNAs...
- MOLECULAR GENETIC ANALYSIS OF MOTILITY: MYOSIN CHAINSRex Chisholm; Fiscal Year: 1990..cDNA clones which encode the Dictyostelium MLCs have recently been isolated...
- Cellular and Biochemical Studies of Myosin Assembly in DictyosteliumThomas Egelhoff; Fiscal Year: 2009..We are using the simple amoeba Dictyostelium discoideum as a model system for identifying signaling pathways that regulate myosin assembly...
- Molecular Basis for Protein-Phospholipid InteractionJon Lomasney; Fiscal Year: 2005..C (PLC), and the small G protein ARF; as a regulator of the actin cytoskeleton directing cytokinesis in Dictyostelium, and acting as a regulator of endo and exocytosis...
- Spatial regulation of cytoskeleton during chemotaxisCHANG CHUNG; Fiscal Year: 2007..neutrophils during wound healing, homing of thymocytes, migration of neural crest cells, and aggregation of Dictyostelium. The first step of chemotactic movement is a chemoattractant mediated increase in F-actin polymerization at ..
- MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUMArturo De Lozanne; Fiscal Year: 2003..Toward that goal, three proteins required for cytokinesis in Dictyostelium have been identified...
- MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUMArturo De Lozanne; Fiscal Year: 2006..Toward that goal, three proteins required for cytokinesis in Dictyostelium have been identified...
- ROLE OF SIGNAL TRANSDUCTION IN DICTYOSTELIUM DEVELOPMENTPeter Devreotes; Fiscal Year: 1992..Recent observations demonstrate that G protein-linked signaling systems are present in microorganisms such as Dictyostelium. Dictyostelium is uniquely suited for studies of chemotaxis in eucaryotic cells and the role of signal ..
- CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSISJames Spudich; Fiscal Year: 2001..Key to a long term undertaking to solve this problem is the judicious choice of a model system. Dictyostelium discoideum is a model of choice because it's behavioral characteristics during mitosis and cytokinesis are ..
- TARGETING OF LYSOSOMAL ENZYMES IN DICTYOSTELIUMJames Cardelli; Fiscal Year: 1993..transport, modification and proteolytic processing of 3 lysosomal enzymes in the simple eukaryote Dictyostelium discoideum, an organism amenable to these studies because of the ease in which it can be manipulated ..
- SIGNAL TRANSDUCTION AND DICTYOSTELIUM DEVELOPMENTPeter Devreotes; Fiscal Year: 1993..pathways play an fundamental role in chemotaxis, cell-cell signaling, and ligand-induced gene expression in Dictyostelium. In the course of its developmental program, this simple metazoan expresses a family of four cell surface cAMP ..


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Well, Robert Downey Jr. is certainly making the most of his second shot at A-list glory. The anchor of the "Iron Man" and "Sherlock Holmes" franchises isn't looking to earn cred by signing on for prestige or indie pics, adding another big tentpole film to his upcoming slate which includes "The Avengers" and "Oz The Great And Powerful."
EW reports that the actor is signed on to a big screen adaptation of "Peabody And Sherman," based on the cartoon shorts that were part of the "Rocky & Bullwinkle Show." In case you are too young to remember, the cartoons centered on a genius dog, Mr. Peabody and his pet boy Sherman, who used the the WABAC Machine to travel through history, learning things along the way. The upcoming film will be a computer animated 3D affair, and while the casting of RDJ to voice Mr. Peabody is inspired, the talent the behind the camera is less than thrilling.
The script was been written by "Yogi Bear" writers Jeffrey Ventimilia and Joshua Sternin, and has Rob Minkoff at the helm. Sure, he directed "The Lion King" and "Stuart Little" but he's also behind "The Haunted Mansion" so, not exactly consistent, then. But he seems to be excited with the task of taking on the film, and sees Mr. Peabody as appealing to the outsider, brainiac dog in all of us.
"Mr. Peabody is this genetic anomaly," Minkoff says. "He does have brothers and sisters, all of them non-speaking, not super-smart dogs. He's an outcast, but has overcome it by being so great at so many things."
The role of Sherman hasn't been cast yet, but work has already started on character design with an image of Mr. Peabody due to be in the pages of EW this week. Excited? You'll have to wait. The film isn't scheduled to hit theaters until 2014.
Ashley | November 20, 2011 7:53 PM
i love this show. i cannot wait to see the movie regardless of who directs it. i just hope they keep those corny little jokes in. they're my favorite :)
GiorgioCanna | January 18, 2011 2:12 AM
"Sure, he directed "The Lion King" and "Stuart Little" but he's also behind "The Haunted Mansion" so, not exactly consistent, then."
Your playlists are the ones that you announced in July 2010 by Wonder Woman, written and directed by David Goyer. Obviously bullshit, but no I do not have confidence in you. It will be a great movie
Marko | January 17, 2011 4:39 AM
"The role of Peabody hasn't been cast yet" . Is this a typo and that it should read "The role of Sherman hasn't been cast yet"?


text: I heard rookie OLB/DE Sam Montgomery came to visit the facility and team yesterday, does anybody know what came of that ? Oh yeah and has anyone seen Sharrif Floyd he's been awfully quiet....thought he was suppose to be the one LOL!? Also, Nnamdi is about to get canned in SF should we bring him back home as well? How many of you believe we can win 10 games this year? I've been claiming that all off season!


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- 16Jul2013
Beautiful Betta Fish as Pets in the Classroom
One of the simplest and most beautiful classroom pets is a betta fish. These gorgeous, vibrantly colored fish live in shallow, warm water in the wild, in the rice paddies of Thailand and Cambodia. The males are more brightly colored than the females, with longer, flowing fins. Two males cannot be kept in the same
- By pitc
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- 16Jul2013
Consider an Aquarium with Pets in the Classroom
Bringing a pet into your classroom is a great way to inspire learning and teach responsibility. There are many options when it comes to choosing a pet, and having information on each of them can make your decision easier. You may consider fish, for example. Adding an aquarium to your classroom is a fun and
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Wordless Wednesday: Disney Love
This week's Disney Almost or Wordless Wednesday theme is Disney Love. The month of romance and love continues. I started my February post with a bunch of lovebirds last week. Well, here are a few more favorites like Cinderella and her Prince.
We can never have enough Ariel and Eric pictures.
Or enough of Mickey and Minnie
From this slumber, She shall wake, When True Love's kiss, The spell shall break…
Happy Valentine's Day to all of you!
If you liked this post, please get the latest posts and updates free. Follow me on Twitter, Google+, or Facebook. Thanks!
A big Thank You to Deb for hosting. Please check the button below for more Disney posts and pictures.


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Drop-in Centre for West Side Neighbourhood Watch (From Worcester News)
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Drop-in Centre for West Side Neighbourhood Watch
1:01pm Wednesday 22nd January 2014 in News
THE West Side Neighbourhood Watch, based in St John's, will be holding a Drop-in Centre on Friday, January 24.
At the Youth and Community Centre, in Swanpool Walk, people will be able to talk to NHS health trainers, members of Hereford and Worcester Fire and Rescue Service and get debt help.
Tea and coffee will be available during the session which runs from 10am until 4pm.


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This past March, I took the longest solo road trip that I had taken. I drove for seven hours to go to Petticoat Junction Retreat Center to hang out with some other bloggers for the weekend. Because I was driving solo, my trip involved trying to make good time and copious amount of Dr. Pepper.
Because of those copious amounts of Dr. Pepper, I decided to stop at a McDonald's for lunch after filling up the van. I needed both more Dr. Pepper and to pretty desperately find a bathroom after having already finished a large McDonald's drink prior to that point of my trip.
In my rush to go to the restroom as soon as I walked in, I hadn't fully processed through what I saw at the register until I was actually in the bathroom. There had been a religious sister at the register carefully counting out her money to pay for her lunch. I thought, irritated at myself for my extreme need for a restroom, "If only I had thought about it sooner, I would have just walked up to the register and asked to pay for her lunch."
My chance at that was gone, however. It had been a nice thought, but I still wish that I had thought of it sooner.
After ordering my food to go, I scanned around the restaurant while waiting and saw this Sister eating alone. While I love being generous when I can be, I instantly had knots in my stomach as I decided what I wanted to do next. I took five dollars out of my purse and stuck it in my pocket.
Could I really just walk up to this woman's table and give her the money? I felt awkward as I walked casually with my drink and bag. I stopped at her table.
"Excuse me, Sister?"
"Yes," she smiled broadly.
And, with some gusto (I think), I plunked the five dollars down on her table. "I would really love it if you would let me pay for your lunch."
She was delighted. "Are you eating with someone?" she asked, even though her eyes moved to my to-go bag. "If you aren't, I would love to have you join me for lunch."
"I'm traveling and was just going to go, actually." But, then I paused. "You know what, Sister? I would really love to have lunch with you. Thank you."
For the next forty minutes, I sat in a southern Indiana McDonalds having the most delightful conversation with this Sister who was based out of the Nashville area. We talked excitedly about the newly named Pope Francis (as this was just the day after that exciting day).
We discussed my family, my children, and our conversion process, as well as the work that they do at her parish, the reasons for her traveling, and more. When it was finally time to go, we got a funny look from a man nearby (who had been hearing us gab like old friends) after we hugged and then told each other that it was so nice to have met each other.
There were so many things that I could have done differently that day. I could have wimped out on walking over to give her the money, and I could have also decided that my drive was too long to sit and have lunch. However, I'm thankful to be able to say that I got to add that extra 40 minutes to my trip. It was truly a gift. I can only hope that she was happy to have added that extra time to her trip too.


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PICTURES: Disney Dream cruise ship
Renderings of the Disney Dream cruise ship( WALT DISNEY CO. / October 29, 2009 )
These renderings are of the new Disney Cruise Line ship the Disney Dream. This is Pixie Hollow in Disneys Oceaneer Club, in which children are transported to the enchanted land of Tinker Bell and her fairy friends. A pixie tree stands in the room with hundreds of fairy lights twinkling among the leaves. In this magical forest setting, children can make crafts just like a true tinker fairy, while sitting on stools shaped like acorns and mushrooms. Other activities in Pixie Hollow include dressing up in fanciful costumes and participating in storytelling. The ship is slated to debut in the spring of 2011 and sail out of Port Canaveral.


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McLean falls in Little League Softball World Series championship
The dream of a Little League Softball World Series title ended for the ladies of McLean Wednesday night in a 9-0 loss to a team from Arizona in the tournament's championship game.
McLean Little League softball team advances to World Series championship
The McLean Little League softball team is set to play for the Little League World Series Championship title Wednesday night in Portland.
McLean Little League softball team advances to World Series championship
All it will take for McLean's streaking Little League softballers on Wednesday is a victory over a team from Arizona - with that, they'll capture the 2013 Little League Softball World Series championship.
Saul Bosquez and Matt Kinsey, Nationals wounded warriors to play against celebritiesComment
Washington Nationals Wounded Warrior Amputee Softball Team players Saul Bosquez and Matt Kinsey will join the star-studded roster for the 2012 Taco Bell All-Star Legends & Celebrity Softball Game this Sunday.
Wounded warrior softball team gets ready to hit homeComment
A one-of-a-kind softball team will take the field Friday for the first time. All of the players are amputees, injuries they sustained as servicemembers.
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The Queen Latifah Show Weekdays at 4pm on ABC7!Watch "The Queen Latifah Show" weekdays at 4pm followed by Leon Harris and Alison Starling on ABC7 News at 5. Click here to find out more about "The Queen Latifah Show!"


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Bruce Evenstad
360 752-8426
Bruce completed the Auto Body Course at BTC (then called BVTI) in 1970 and started working at Deihl Ford were he served as a Auto Repair Technician. Bruce also served on the Auto Body Advisory Committee. Bruce Left Diehl Ford to become the Auto Body Instructor at Bellingham Technical College and has been teaching the Auto Collision Technology Course for 34 years. During his tenure he has become the Certified I-Car Instructor ,an I-Car Platinum Individual Certificate, PPG Master Refinishing Technician and completed the Car-o-Liner vocational instructor course for measuring and straightening unibody and frame vehicle
Degrees and Certifications
Auto Body Certificate, Bellingham Technical College
PPG Certified Technician Master Level
I-CAR Certified Instructor
Certified Refinish Technician for PPG Level Three
Certified Journeyman
WA Professional Technical Certification


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Former UFC Heavyweight champion Josh Barnett is the most high-profile "ronin" coming out of Strikeforce. He was not one of the 20-something fighters that was announced as heading to the UFC from the now defunct organization and many speculated that his high asking price (he made $250,000 for his final SF bout) and his infamous troubles with performance enhancing drugs and getting along with Zuffa made him an unlikely candidate for a return to the UFC.
That may have been premature.
MMA Mania's Mike Bohn spoke to White at today's media scrum and Dana said, "I have not (spoken with Barnett). It's probably going to happen."
Exciting news for fans of the baby-faced assassin who has not fought in the UFC since defeating Randy Couture back in 2002. Barnett never fought for a title in his Pride tenure but he was consistently near the top of the division and he finished second in the Strikeforce Heavyweight Grand Prix in 2012.


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'Time to accept the worst' for missing sailors (From Swindon Advertiser)
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'Time to accept the worst' for missing sailors
Updated 2:02pm Saturday 24th May 2014 in News
THE uncle of Steve Warren, who was onboard the yacht Cheeki Rafiki when it capsized, has said the family are taking comfort knowing he was doing something he loved when went missing.
Albert Davey, 76, of Royal Wootton Bassett, believes following the discovery of the wreckage with the life raft still attached, hope of finding his nephew alive has gone.
The 40-foot boat was sailing back from a regatta in Antigua last week when it started to take on water in the North Atlantic. On May 15 the crew contacted the boat's owners in Southampton to say they were in trouble which was the last contact.
It was hoped the four man crew had escaped in the life raft but on Friday the yacht was discovered overturned in the water with the raft still attached, dashing the theory they were still adrift in the ocean.
Albert said: "It has been a rollercoaster of a week but I think after the divers found the life-raft still attached the time has come to accept the worst has happened.
"It is not the outcome any of us wanted but we are all taking comfort from the fact they were all doing something they all enjoyed.
"They had just won in their class in Antigua and the crew was elated. It was a case then of bringing the boat back home.
"We can only assume that something catastrophic happened in the middle of the Atlantic when the weather conditions were at their worst, with 15-foot waves and strong winds."
Albert described Steve, 52 and the father of two daughters, as someone who was much-loved by the whole family.
He said: "Steve will be missed by everyone. What we can't do is get angry at Steve for doing something he enjoyed.
"A lot of people enjoy doing something which has an element of risk but that shouldn't stop us.
"We didn't want it to end this way but at least this is an end."
Steve, of Bridgewater, went missing with other crew members Andrew Bridge, 22 from Farnham, Paul Goslin, 56, from West Camel, and 22-year-old James Male, from Romsey.
The US Coastguard initially called off the search off last Sunday but following a campaign from the families the search was started again on Tuesday, with the help of the RAF.
"What the family would like to say is thank you to the public and the media who have given us so much support and continue to do so," said Albert.
"Because of them, the search was restarted which gave us hope and we are very grateful for that."
Speaking earlier a spokesman for the Coastguard said: "The US Coast Guard has confirmed the life raft aboard the capsized sailing vessel Cheeki Rafiki was secured in its storage space in the aft portion of the boat, Friday, indicating it was not used for emergency purposes."
Comments are closed on this article.


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The Cape Film Commission (CFC) has announced that the long-running American TV police procedural, Law & Order, is coming to South Africa, to be titled, what else but Law & Order: Cape Town.
12 episodes have been ordered with filming set to begin on location, in Cape Town, by May of 2012, with a cast and film crew that will be primarily South African.
Hopefully the cast will be diverse. I'd fully expect to see black South Africans represented in leading roles.
Geraldine Singh | December 15, 2011 2:16 AM
Awesome :)
liza | December 13, 2011 4:22 AM
how can i audition for this if i dont have an agent? does anybody have any details about the auditions? please let me know.
Cape town Actor | November 28, 2011 5:45 AM
Exciting indeed...Im lucky to have a good agent and look forard to the chance to audition. I have been typcast a bit as a cop so maybe its a good thing for once :-)
Prince | August 23, 2011 1:49 AM
Geneva Girl | August 20, 2011 8:20 AM
I am so excited. I'm a L&O junkie. I've watched Criminal Intent Paris (especially difficult because Parisians talk very fast with lots of slang and there were no English subtitles). I also love L&O UK.
I'm just hoping that this version will end up on one of the South African channels I get on Sky. And, no, I haven't gotten hold of the Moscow version yet, but I doubt it's available with English subtitles.
I just wish that the new versions would use original stories. They're just localized versions of old L&O episodes. It is interesting, however, to see how the different legal systems work.
Adam Scott Thompson | August 20, 2011 4:40 AM
NaijaPrincess | August 20, 2011 3:07 AM
What, no L&O: Lagos? LOL....


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Monday night saw the kick off of the 'QT Fashion week and Awards' held at the QT Stingray bar Surfers Paradise. This annual week long event showcases the best of the best in Australian fashion and this year is proving to be the most exciting one yet with runway shows by, White Sands, Lisa Brown, Nicola Finetti, Ellery, Dion Lee and my absolute God of all Australian fashion God's Josh Goot!
|Photo credit - http://thecompanyswoon.com
Ksubi AW13 Brocade peplum top/skirt set
Yves Saint Laurent Clutch
Balenciaga cuff
Gucci 'Devon' booties
With shows running all week be sure to book your tickets through http://www.moshtix.com.au/v2/ for more information visit the official QT Fashion week facebook page here -


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Carolina Live!
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Marian McPartland's Piano Jazz
Saturdays at 8:00 PM on ETV Radio's News Stations, Sundays at 7:00 PM on our Classical Stations
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Song Travels with Michael Feinstein
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Walter Edgar's Journal
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The Other Brother
Walter Edgar's Journal - Friday at noon; repeats Sunday at 4:00 PM on our News stations
The Other Brother is a film about the 'genetics' of art and sibling estrangement. The subject is art but the story is universal. Two brothers, estranged since 1948, share an exceptional bond. One is an art-world insider, and one lived alone in a world of art. Artist and educator Tom Flowers, his son, artist Mark Flowers, and Mark's wife, artist and filmmaker Kristy Higby talk with Dr. Walter Edgar about The Other Brother. More...
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Mobile users, you can get both our Classical Stations and News Stations streams through Public Radio Playerr, the NPR News mobile app, and Tunein. iTunes Radio users can listen to our News stream! Just search for "NPR News - ETV Radio."
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We could do a million stories on the beauty of this state. From the rolling mountains of the Upstate to the beautiful seas of the Low Country. Our state is truly unique, but what really makes South Carolina sweet is its amazing people. The people are what make South Carolina original. More...
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The Uprising of '34 and the Rediscovered Story of Bloody Thursday by Honea Path Native Frank Beacham
The Uprising of '34 airs on SC ETV on Thursday, Sept. 4, at 9:00 p.m.
In 1934, Southern textile workers took the lead in a nationwide strike that saw half a million walk off their jobs in the largest single-industry strike in the history of the United States. For a time, these new union members, in response to New Deal legislation, stood up for their rights and became a force to be reckoned with in the South. Then management moved in and crushed the strike. Some mill workers were murdered, thousands more were blacklisted, and many were so intimidated that "union" became a dirty word in Southern communities for decades to come.
Barely publicized, rarely acknowledged in history books, the General Textile Strike of 1934 remains a stirring, yet amazingly forgotten, chapter in Southern history. The Uprising of '34 (POV, Thursday night at 9:00 on ETV) is a film by famed documentarian George Stoney and independent filmmakers Judith Helfand and Susanne Rostock that examines this hidden legacy of the labor movement in the South and its impact today.Frank Beacham, journalist and author, has a personal connection with the uprising of 1934. One chapter of his 2003 book Whitewash: A Southern Journey Through Music, Mayhem, and Murder (Booklocker, 2002) tells of Beacham's discovery of his grandfather's involvement the the suppression of the strike by mill workers in his hometown of Honea Path, SC. He talked with historian Walter Edgar on Walter Edgar's Journal in 2003. Listen to or download the podcast here.
Classical Unknown
Thursdays at 9:00 p.m. on ETV Radio's Classical Stations
Classical Unknown, host Eva Bennett, brings you the unsung and lesser-known composers of classical music. The program, a new production from ETV Radio, explores hidden performances as Eva searches throughout our vault of recordings of artists and their music that are rarely heard. More...
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Radio Reader: Where to Listen
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Listen to the 2013 - 2014 season of the Metropolitan Opera Saturday Matinées, streamed by NPR member station WQED, Pittsburgh. (Once you open the page, click the "Now Playing" icon.) Check the Met's schedule for weekly listings and start times.


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Videos > Bailey Lane > Young slut Bailey Lane feeds her hungry mouth with an awesome meatpole
Innocent High Watch as these Innocent High girls have to face Principal Fitsergood. There is only two ways to pass in his school & both involved getting fucked!!
Featured Pornstars:
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I update when I can. Sorry. Not sorry. Actually, sorry O_O -17! -I can drive now! (if only I had places to go...) -I'm pretty awesome (if I do say so myself XD) -No, I lied, I'm a DELIGHT -I don't like boys; I like men. -I like math (plz don't beat me up) Votes 1000 [x] 1100 [x] 1200 [x] 1300 [] 1400 [] 1500 [] 1600 [] 1700 [] 1800 [] 1900 [] 2000 [] Fans 100 [x] 110 [x] 120 [x] 130 [x] 140 [x] 150 [x] 160 [x] 170 [x] 180 [] 190 [] 200 []
Good Luck KissA school that doesn't have anything going for them, no top SAT scores, no brainiac genius to invent the next smart phone. Except one kid; the quarterback of the football tea... read more
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The Diary of the DevilThe only way Greyson Skinuth could begin to describe his long time bully is as the devil. The devil is supposedly the most evil creature in all of the universe. And ... read more
Once Upon a Time... (on hold)Once upon a time, there was a boy. Boy's name is Donnie. Boy meets girl. Girl's name is Natalie. Boy's world get turned upside down. He is blinded by his love for ... read more


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Forum / Strictly Steam / New Steam Gas Heating System
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New Steam Gas Heating System (8 Posts)
Bklyn December 2, 2010 @ 2:29 PM
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New Steam Gas Heating SystemThis past summer i had my oil burner replaced with a gas burner In a 3 story row building. What i have noticed that on the top floor in the bathroom i hear the air escaping from the # D air valve, and hear the steam rising, however the radiator does not get warm. It seems that the Thermostat that's located on the second floor reaches it temperature before the steam can rise into that radiator, can you help me with this? Also my living room radiator on the top floor does not get hot all the way, just seems to get hot half way? I keep the thermostat set at 73 degrees, but when i bump it up to 78 (just to see what happens) Then all the radiators on the top floor get hot.
Thank you for your time Brooklyn
nicholas bonham-carter December 2, 2010 @ 3:40 PM
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slow top floormake sure that your main vents [not rad vents] are capacious+1, and check the anticipation on your thermostat.--nbc
Big-Al December 2, 2010 @ 5:54 PM
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Slow Vent Near the StatWhat kind of vent is on the radiator near the thermostat? Maybe #5 or even a #4 on that radiator will help. Sometimes it's not enough to vent the cold radiators faster . . . You might need to vent the hot ones slower.
You might try a #4 Jacobus Maid-O-Mist vent first. It will have a removable orifice that can be easily unscrewed and drilled out a little bigger if you discover it's actually too slow.
And, yes, good main venting makes a big difference.This post was edited by an admin on December 2, 2010 5:56 PM.
jpf321 December 2, 2010 @ 6:07 PM
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as Al said...try to throttle the rads that effect the t-stat a bit until you find a happy medium. I am in the process of playing around with TRVs so that rooms max-out and drop out of the load equation once they are to temp. Which means that I have throttled my near-t-stat rads way down.Entire Site | MAIN WALL | Strictly Steam | Off-Wall
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installed: 0-20oz/si gauge | vaporstat | hour-meter | gortons on all rads | 1pc G#2 + 1pc G#1 on each of 2 mains
Connected EDR load: 371 sf venting load: 2.95cfm vent capacity: 4.62cfm
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Steamhead December 2, 2010 @ 6:29 PM
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Whydo you have such a big vent on that radiator? Do other radiators have the same vents on them?
Look at the ends of your steam mains. Are there vents installed there?"Reducing our country's energy consumption, one system at a time"
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Big-Al December 3, 2010 @ 10:30 AM
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Affects all the radiatorsPutting slower vents on the hot radiators doesn't just slow those ones down . . . it can speed up the other ones as a side effect. (The steam has to go someplace.)This post was edited by an admin on December 3, 2010 10:31 AM.
nicholas bonham-carter December 2, 2010 @ 8:59 PM
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steam secretsbad main vents=bad heat. do the mains first, and wait until you have got that right before spending any money on the radiator vents. the radiator vents are just not able to handle the system air removal needs, so spending money on them first is usually wasted.--nbc
JohnNY December 8, 2010 @ 9:28 AM
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Boiler too small?Was a proper Manual J, or EDR calculation done to size the replacement boiler?
I'd be happy to come take a look if you're within my service area in Brooklyn. Please use the Find a Contractor feature at the top of the page or click the link below to learn more about my company.


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Locate an ACVR Radiologist or Radiation Oncologist in your area.
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2 bed, 2.00 full bath. Prime Fairhaven Location-close to everything. This end unit features high ceilings, open kitchen w/More info
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1 bed, 1 full bath. Lovely studio condo! - Bellingham Corner unit w/ private pa- tio & view. NEW windows, heatiMore info
, 1.00 full bath. Lovely studio condo! Corner unit with private patio and nice view~ You have access to Sudden ValleyMore info
2 bed, 1.00 full bath. Perfect! 2 bdrm 1 bath sits against the lush green grass along the forests edge, A never ending disMore info
2 bed, 1.00 full bath. Top floor, corner unit located in desirable Atrium Building in the heart of downtown Bellingham. FrMore info
3 bed, 1.50 full bath. Single story end unit condominium close to the bus network & walking distance to shopping. This rarMore info
2 bed, 2.00 full bath. Great afternoon sun exposure plus view of club house and grounds. New carpet and paint-fresh and reMore info
2 bed, 2.00 full bath. Luxury Penthouse located on the Southwest corner of the building, filled with light, and panoramicMore info
, 1.00 full bath. Great golf course-view studio condo in beautiful Sudden Valley. Efficient use of space throughout--More info


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Luren does amazing display installations and is branching out into weddings. I love this cloud of hand made wax paper flowers hanging over the guests. Contact Luren through her tumblr.
oooh … so pretty! you could wear the tsumori chisato dress to a party like that.
Gorgeous party installation, I'm inspired.
[...] Bird & Banner This entry was written by Courtney, posted on May 4, 2010 at 7:00 pm, filed under Art and [...]


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Public Programmes
Schools, COLLEGES AND Universities
With a remarkable art collection which stretches from the early Renaissance to the 20th Century and an acclaimed programme of temporary exhibitions, The Courtauld Gallery is a stimulating and enriching learning environment.
Our innovative, wide-ranging programme of art history, cross-curricula and art practice activities for Schools and Colleges is underpinned by the principle: "All art was contemporary once."
Complementing and extending Art and Design and Humanities curricula, the gallery learning programme develops students' visual analytical skills and critical and contextual understanding increasing their confidence and enjoyment of looking and talking about art. Workshops, talks and projects are available on a broad range of topics and content can be tailored to fit with your current study theme.


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Houzz is the new way to design your home.
3 Ideabooks by Caitlin Creer Interior Design
caitlincreer's Favorites
19 photos
Guest Picks: Hermes Has It Right
23 photos
Orange isn't just for Halloween anymore. I think Hermes is on the right track with their pretty orange boxes that have just the right amount of sophistication and warmth. Orange can be a playful and exciting way to add some pop to your decor — just...
Guest Picks: Faux Bamboo Furniture
20 photos
Let's just say I never met a piece of faux bamboo furniture I didn't like. The lines and texture are a great way to establish an eclectic look without going too far out of the box. There's been a resurgence of faux bamboo pieces on the market lately and...


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I am bored and sober so i thought I would reach out to the Raider Nation and ask the following:
1. Who are the top 5 best posters on Silver & Black Pride?
2. Who are the top 5 worst posters?
3. Who are the top 5 funniest posters?
4. Who are the top 5 Raiders you enjoy watching the most?
5. Who are the worst 5 Raiders you loathe watching?
6. What are the estimated weights of the 5 biggest women you ever slept with?