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Funding opportunities
Establishment of Human Embryonic Stem Cells Modeling Human Disease by Somatic Cell Nuclear Transfer
Funding Type:
New Cell Lines
Grant Number:
Funds requested:
$1 532 277
Funding Recommendations:
Not recommended
Grant approved:
Public Abstract:
To date, most basic and applied research on human disease is performed with animal models, the majority being mice. Unfortunately, most of what is learned from mouse models is not associated with human disease. Moreover, even with current advanced genetic technology, it is nearly impossible to generate models of rare diseases such as Amyotrophic Lateral Sclerosis (ALS), also known as Lou Gehrig's disease. "Therapeutic Cloning" will provide a novel opportunity to study patient specific stem cells allowing one to understand the genetic causes of disease. It is a process known as somatic cell nuclear transfer (SCNT) in which the nucleus of a donor cell is transferred into an egg that has been emptied of its chromosomes. That egg now contains an exact duplicate of the donor's genome, and if all goes well when it is cultured in incubator for five to seven days, an SCNT-embryo will develop into a stage in the incubator for derivation of embryonic stem cells (ESC) which can differentiate into any cell type a patient may need for possible therapy. However, although the potential of combining SCNT technology with ESC research for the future therapies in animals has been demonstrated, it has not yet been accomplished in humans, although members of our team are closing in on this accomplishment. While we understand and respect those who oppose SCNT-ESC research, we are equally sincere in our belief that the life-and-death medical needs of suffering children and adults might be ameliorated by insights derived from SCNT-ESC. This proposal marks the beginning of the effort to use human embryonic stem cells (hESC) in a series of experiments whose principle has already been proven in animals and even in early stages in human by members of our team. The focus of our research is to gain a better understanding of ALS. By developing ALS hESC lines, particularly recreating the neuronal niche thought to be awry, researchers will have a novel tool for studying the mechanism of disease. Furthermore, this presumed new model for ALS may also be used to test potential therapies; high throughput screening allows the rapid examination of millions of compounds and may be used to examine gene manipulations and it's impact on disease. Members of our team have recently reported the first successful derivation of SCNT-embryos from cultured adult cells (skin) in the human. Other members have derived new hESC cell lines under very defined conditions and feel poised to accomplish the next step; the derivation of SCNT derived hESC lines. Upon completion of this project, we may be one step closer to understanding and perhaps stopping the progression of this horrific disease.
Statement of Benefit to California:
The proposed research involves three cutting edge technologies: human embryonic stem cell research, somatic cell nuclear transfer, and patient specific cell lines. These technologies which are complementary to each other, have yet to be developed and optimized in humans, and when combined, will offer great potential in medicine. The ability to stay close to emerging technologies is paramount to keeping California business at the leading technological edge. In addition to the likely economic benefits to the State of such a commercial program, it is critical to point to the paramount medical benefits to the public of such collaboration in terms of the development of milestone medical therapies for future cell therapy. Upon completion of this project, we will be able to prove the concept of SCNT-ESC research as a tool for developing therapies. Stem cells derived from SCNT-embryos will likely be a conduit to drug development, eventually leading to new pharmaceuticals. Furthermore, producing such stem cell lines would establish a novel resource to study the role of individual genes in disease development. With the improvement of cloning methodologies as described in our proposal, SCNT-ES cells could be used as a cellular transplantation resource. Cell therapy technologies overlap with those of gene therapy, cancer vaccines, drug delivery, tissue engineering and regenerative medicine. Collectively, the value of the cell-based markets is estimated to be $26.6 billion in 2005, $56.2 billion in 2010, and $96.3 billion in 2015. The largest expansion will be in diseases of the nervous system and cancer. Skin and soft tissue repair as well as diabetes mellitus will be other major markets. The number of companies involved in cell therapy has increased remarkably during the past few years. In 2006, more than 500 companies have been identified to be involved in cell therapy. Of these, 104 are involved in stem cell therapy. If the proposed research is successful as anticipated in contributing to the development of cell therapy products, there are likely to be substantial benefits to two critical sectors of the California economy, health care and pharmaceuticals. Additionally, our proposed research will benefit through further refinement and expansion of its SCNT-ESC technology and through collaboration in other areas of biotechnology, including molecular biology and immunobiology. Furthermore, the advancement of ESC research together with SCNT technique will enhance partnership-developing programs for start-up as well as established biotechnology companies. Support for this project will help maintain our leadership in embryonic stem cell and cloning technologies and will help a California business to be better equipped to compete for a multi-billion dollar market.
Review Summary:
Executive Summary This applicant proposes to develop methods to generate human embryonic stem cell (hESC) lines using somatic cell nuclear transfer (SCNT), in order to allow the derivation of disease-specific cell lines. The applicant states that nuclear transfer is the "gold standard" method of reprogramming and will remain that way until proven otherwise. Optimization of SCNT technology to humans is described in Aims 1 and 2. Once SCNT methods are reliable in humans, the applicant proposes to derive hESCs from individuals with known genetic versions of motor neuron disease (amyotrophic lateral sclerosis, ALS), as well as patients with idiopathic motor neuron disease. This is an interesting and highly focused application with two goals 1) to develop SCNT in humans and 2) to study motor neuron disease. The recent publication of the derivation of non-human primate ES cells by SCNT certainly advanced knowledge on ES cell derivation. To be able to accomplish this with human cells would be an important breakthrough. Even if transcription factor-mediated somatic cell reprogramming becomes the technique of choice, a comparison between that method and SCNT-mediated reprogramming may yield useful data for making reprogramming more efficient. In addition, if successful, this application could lead to the generation of hESC encoding important genetic information regarding the pathogenesis of motor neuron disease, which could provide new therapies specific to this disease. In spite of the potential significance of the work, and therefore its potential fundability, reviewers were not supportive of this application due to significant weaknesses in the project and the lack of experience of the principal investigator on the project. Reviewers were very critical of the fact that, if Aim 1 fails to produce blastocysts from SCNT embryos, Aim2 and 3 cannot be performed. Overall, the preliminary data presented was weak and the project was poorly described. Aim 1 is an assembly of different protocols for oocyte activation and delivery of the nucleus into the oocyte, with no sound rationale other than a handful of manuscripts gathered from experience in farm animals. The applicant describes the use of chromatin transfer but there is no explanation of how this is done or reference of work done by the applicant or some of the collaborators. In Aim 2, the applicant is proposing to use growth factors to enhance the development of the SCNT embryos. The applicant proposes to use factors that favor the development of fertilized embryos, but does not take into account the work done by others that demonstrated that SCNT embryos need culture systems that resemble more those from somatic cells rather than embryo culture. The availability and source of oocytes was also of concern. Reviewers were unclear about how many oocytes were needed, how many oocytes would be used in each aim, and how many of these oocytes would be used fresh or be supplied frozen. This information impacts the overall budget of the proposal and the statistical power of the results. Furthermore, one reviewer commented that the applicant did not provide evidence that s/he has access to a supply of oocytes, as the applicant acknowledges this is a limiting factor. Reviewers were also concerned about whether the project was feasible as described. As the applicant points out, the group has considerable experience in the technology to be used and much of the proposed work seems standard. However, although the project lists good and appropriate collaborators, the role of the collaborators is not clear. In addition, the applicant appears to have no experience managing a major project. The applicant also failed to cite key publications on motor-neuron disease, perhaps reflecting lack of understanding/knowledge of the field. Finally, one reviewer noted that although the gene expression and proteomic analyses they plan on neurons derived from ALS patients with familial and sporadic disease might identify significant differences, it could equally yield a long list of gene expression differences that will be hard to decipher. Reviewer One Comments Feasibility: Strengths: - PI has some experience with embryo manipulations and oocyte activation - One of the collaborators recently published the first human embryo produced by SCNT Weaknesses: - Aim one is an assembly of different protocols for oocyte activation and delivery of the nucleus into the oocyte with not sound rationale other than a handful of manuscripts gathered from experience in farm animals. - Aim 1.2 describes the use of chromatin transfer but there is no explanation of how this is done or reference of work done by the PI or some of the collaborators - How many oocytes will be used in each aim? How many will be used fresh vs. frozen? How many are needed? This will impact the overall budget of the proposal and the statistical power of the results. At least the PI should have included the desired number and worked from there. - Aim 2.2. The PI is proposing to use growth factors to enhance the development of the SCNT embryos. It is proposed to use factors that favor fertilized embryos to develop. PI does not take into account the work done by Latham's group that demonstrated that SCNT embryos need culture systems that resemble more those from somatic cells rather than embryo culture. - If Aim 1 fails to produce blastocysts from SCNT embryos, Aim2 and 3 cannot be performed. Responsiveness to RFA: yes Reviewer Two Comments Significance: The goal behind this proposal is to establish hESC lines from blastocysts using SCNT. The applicant proposes that this method is the "gold standard" and will remain that way until proven otherwise. If reprogramming becomes the technique of choice, a comparison between the two methods may provide useful data in making reprogramming more efficient. The publication of the derivation of non-human primate ES cells by SCNT by Byrne et al (2007) certainly advanced knowledge on ES cell derivation. To be able to accomplish this with human cells would be an important breakthrough. The question however will be whether iPS cell technology really takes over the field, making SCNT redundant. However this remains to be proven and our current understanding of hESCs and their differentiation potential means that iPS cells have to catch up. As the applicant points out, the group here has considerable experience in the technology to be used and much of the proposed work seems standard and not necessarily innovative. Feasibility: Much of this proposal is technically rich from a group of investigators who know what they are doing in cloning and SCNT in rodents and in large animals. They have three Specific Aims which seem well organized and appropriate. It might have been helpful to have a letter of collaboration from an IVF clinic ensuring them of a supply of oocytes as they acknowledge this as a limiting factor. Throughout the Research Design they provide alternative approaches if the initial plan does not work. I could not find any note however of their using any of the techniques used by Byrne et al in their paper – could they not learn something from this study? They have chosen ALS as the disease that they would like to model with their hESCs. The Co-PI, Dr. Snyder has considerable experience with in vitro neural stem cell differentiation. Unless I have missed this, they did not cite the two papers from Nature Neuroscience on mutant astrocytes in the SOD-1 transgenic mice that were derived from ES cells. The co-cultures they note here are similar to the studies in Nature and should have been cited. It might be that the gene expression and proteomic analyses they plan on neurons derived from ALS patients with familial and sporadic disease might identify significant differences or simply a long list of gene expression differences that will be hard to decipher. The preliminary data that they present is dense and somewhat disorganized getting down to the size of figures where over-strength reading glasses are called for! It would have been much better to organize this data according to the Specific Aims. The team is strong and they are technologically accomplished. The PI is a junior Research Assistant Professor. He has some quite significant publications in the field but no funding at present. While they name a number of senior scientists in San Diego and elsewhere who are collaborators, letters of support are missing. I wonder what exactly I. Wilmut will actually do here, apart from providing his name. It also might be noted that I. Wilmut has publicly given up on SCNT as a means of deriving disease-specific hES cells in favor of the iPS approach. Responsiveness to RFA: Yes, they will demonstrate pluripotency of their cells appropriately and share them. Reviewer Three Comments Significance: The work in this application is focused on the generation of motor neuron disease specific cell lines in patients with both genetic and non genetic forms of ALS. If successful, this application could provide human embryonic stem cells encoding important genetic information regarding the pathogenesis of motor neuron disease which could provide new therapies specific to this disease. Feasibility: This is a highly focused proposal specifically targeted on the generation of motor neuron disease specific human embryonic stem cell lines using somatic cell nuclear transfer. The applicants have provided a relatively precise and detailed methodology for the generation and characterization of the resultant cell lines. Moreover, they have also provided detailed methodology and preliminary data regarding differentiation of these cells toward neural lineages. To date no group has successfully generated cloned human embryonic stem cells through SCNT although two of the lead applicants are authors on a recent publication demonstrating successful nuclear transfer and propagation of cloned embryos to the blastocyst stage. Overall the applicants would appear to have the necessary research expertise in the various required aspects of the project including cloning technology, neurobiology, and human embryology. However the principal applicant is a very junior research scientist, currently holding a research assistant professor at the Burnham with no demonstrated ability to manage a project of this size. It is very unclear why he is leading on this project. Another criticism is that the role of Professor Yang and Professor Wilmut in this application are ill-defined. Responsiveness to RFA: Assuming that the applicants can successfully generate expandable populations of cells derived from patients with motor neuron disease, the characterization and differentiation of these cells as outlined in the application is appropriate. Moreover the applicants have considerable expertise in neural differentiation of human embryonic stem cells, as well as other non-neural populations. Unfortunately in this application there does not appear to be any consideration of how these cell lines will be provided for use by other research groups and/or commercialized, with the exception of a single line that says "All reagents and products will be made available to research groups cost free."


text:
Power cut in Shrub End (From Essex County Standard)
Get involved: send your pictures, video, news and views by texting ECS to 80360, or email »
Power cut in Shrub End
5:27pm Wednesday 5th February 2014 in News
ENGINEERS are working to fix a power cut in Shrub End.
More than 180 homes have been without power since 4.50pm.
Homefield Road and Layer Road are among those affected.
UK Power Network engineers are working to restore the service.
A spokesman from the company said: "We would like to reassure our customers that we will be working as quickly and as safely as possible to restore power as soon as we can.
"We would like to apologise for any inconvenience caused by this incident."
Comments are closed on this article.

subset: commoncrawl


text:
French lighting manufacturer Robert Juliat has opened an international center of operations in Connecticut. The goal of the new venture is to provide timely delivery and direct service to the US market and to get feedback from customers and users to drive product enhancement and development.
The US operation is managed by industry veteran Fred Lindauer and includes a sales office and a warehouse stocked with finished products and replacement parts.
The new contact information is Robert Juliat USA, 48 Capital Drive, Wallingford, CT 06492, phone: 203-294-0481, fax: 203-294-0482, e-mail: email@example.com.


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No matter which team you root for, watching one of the NFL's best runners back on the field in the season opener after a tough ACL injury is a beautiful thing. And here is Adrian Peterson scoring his first touchdown of the year.
I was able to get an exclusive interview with Jacob Tettleman of Palm Beach, FL, who owns Peterson in a Yahoo! league. He said, "Oh, cool, why doesn't AP have three touchdowns? The Jaguars suck."


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Gentoo v2006.0
Published on 26 February 2006 01:00 AM EST
Written by
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Operating Systems
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The announcement has just come down that
Gentoo 2006.0 has been released
. Packed in this first release for the year is KDE v3.4.3, GNOME v2.12.2, XFCE v4.2.2, GCC v3.4.4, and the Linux 2.6.15 kernel. Also in this release are improvements for 32-bit and 64-bit userlands as well as optimizations for G5 and POWER5 processors. There are also vast improvements to the Gentoo v2006.0 LiveCD. A list of mirrors is available
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Haiku OS Gains Rudimentary Support For Haswell Graphics
Genode OS 14.08 Has New GUI Architecture, Pluggable VFS
MenuetOS Updated With SMP Threads & Onscreen Keyboard
The Widely-Criticized New Commercial Linux Distro Is Now On Kickstarter
Scientific Linux 7.0 Beta 3 Released
Mageia 5 Alpha 2 Works To Add KF5 & LXQt Packages
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A Pennsylvania man was arrested following an investigation into reports that he exposed and touched himself during a job interview in Milford last year.
A complaint was received on Aug. 9, 2012, from a woman that said she had an inappropriate encounter with Armandito Ortiz, of Pittsburgh, during a job interview at Inventhelp, located at 57 Plains Rd., Milford.
She told police he exposed himself and then began to touch himself during the interview.
The investigation led to an arrest warrant being issued and executed for Ortiz.
He was charged with disorderly conduct.
Copyright 2013 WFSB (Meredith Corporation). All rights reserved.


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Cast of Rebecca Mourns Show's Collapse; Middleman Between Producer and Lost Investor Named
By Kenneth Jones
The collapse of the $12 million musical Rebecca in the days before rehearsals were to begin on Oct. 1 was mourned by the cast and staff at a meeting called by the producers. The suddenly unemployed troupe gathered on what would have been their meet-and-greet day.
The meeting was not mandatory, of course, but lead producers Ben Sprecher and Louise Forlenza extended the invitation.
Sprecher then said he was blindsided on Sept. 28 by a malicious anonymous tipster who scared the new investors off, scuttling the production's hope of a fall launch.
On Oct. 2, the New York Times reported that the man who introduced Sprecher to Paul Abrams was Mark C. Hotton, a Long Island businessman who has been sued for fraud in federal court in New York and Florida. The paper reported that Hotton filed for personal bankruptcy in 2011. His debt was $15 million.
The U.S. Attorney's Office and the FBI are reportedly looking into the circumstances of the loss of financing of Rebecca, including the identity of Abrams. A call to Sprecher's spokesman, lawyer Ronald G. Russo, was not immediately returned on Oct. 3.
News reporting about the loss of one-third of the show's budget left some to think that Abrams was a fictional character. Sprecher told the New York Times on Oct. 2, "I never made up or fabricated any investor, and I never made up Paul Abrams."
Of the Oct. 1 company meeting, cast member Donna English wrote on a Facebook posting, "Yesterday the entire cast as well as the creative team and producers came together on what would have been our long awaited first day of rehearsal. Although the meeting may have begun with skepticism from some, no one was there with pitchfork in hand, ready to string someone up. This is an amazing group of people who have bonded together over these past months, and the love and sadness in the room was palpable.
"Four of our producers were there, as well as our esteemed director, choreographer and writers, all of whom were very supportive of our lead producer, Ben Sprecher. Ben, who was distraught, was very forthcoming in telling us of the events over these past few weeks, ending with the most recent and disturbing case of wire fraud that is now under criminal investigation. He offered us a chance to ask questions, and he answered those questions."
English wrote that Sprecher "and [director Michael] Blakemore shared their continued belief in the show and in us as a company, and Ben vowed he would not give up. The meeting ended with a general feeling of immense warmth and support (including a standing ovation for Ben as he left to go meet with the Shuberts), and the hope that we would all meet again under happier circumstances."
Cast member Roger DeWitt told Playbill.com that the Oct. 1 meeting was "an experience in solidarity," admitting, "We're still all grieving."
The three cast members all expressed a wish that the public reserve judgment about the circumstances about Rebecca's collapse until all of the facts are known.
"Hold up your fellow actors," English wrote." Hold up producers who take this crazy gamble because they believe in a theatrical project so much. Give the benefit of the doubt instead of joining in the negative drumbeat of innuendo and rumors."
DeWitt said, "People tend to treat shows like entities, they become depersonalized and it's fashionable or fun to throw pot shots at it."
On Oct. 3, the cast of Rebecca was meeting with Actors' Equity Association to learn what the actors' rights are. At least, the bond that Equity requires of producers guarantees them two weeks of pay.
On Oct. 2, Equity told Playbill.com, "Actors' Equity Association is working diligently with the producer of Rebecca to ensure the cast is paid properly."
Read more about the circumstances of the demise of Rebecca here.
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AACC Celebrates 49th Commencement With Record Numbers
Related press release - AACC Expects to Set Records at 49th Commencement
page 1 of 2
Nancy S. Grasmick, Ph.D., state superintendent of schools, delivered the keynote address. Grasmick is the longest-serving appointed state schools superintendent in America. The Baltimore native, who plans to retire June 30, 2011, has led the Maryland K-12 system for two decades. Grasmick made history in 1991 as Maryland's first woman selected to serve as state superintendent of schools.
Active on area boards and commissions, Grasmick has served two decades on the Governor's Executive Council, Maryland Education Council and executive committee of the Governor's Workforce Investment Board. Other memberships include the Johns Hopkins University Center for Talented Youth Board, Maryland Business Roundtable for Education, secretary-treasurer of the Maryland State Board of Education, and the President's Commission on Excellence in Special Education.
Students honored for academic achievements included an estimated 164 associate degree recipients who earned summa cum laude honors with a grade point average of 3.8 to 4.0, 135 associate degree recipients who received magna cum laude honors with a GPA of 3.6 to 3.79 and 205 associate degree recipients who earned cum laude honors with a GPA of 3.4 to 3.59.
Valedictorian: U.S. Army Staff Sgt. Huggins-Hodge
Valedictorian U.S. Army Staff Sgt. Dysha Huggins-Hodge, who earned an Associate of Arts degree in Transfer Studies, achieved much of her 4.0 grade point average through online classes while deployed in Afghanistan and stationed at Fort Meade. She served as a chemical, biological, radiological and nuclear specialist who traveled to posts to update personnel training and ensure stations were properly prepared in case of hazardous materials incidents.
Huggins-Hodge used Skype software to take exams and complete class assignments when off duty. She attributes her good grades to the discipline and maturity she learned in the Army. She plans to earn a bachelor's degree and a doctorate in social work and specialize in helping teens ages 18 and under.
Presidential Scholar, Student Marshals
Selected as the 2011 Presidential Scholar is William Christian Capuozzo of Glen Burnie, who earned an Associate of Science degree in the Engineering Transfer program. The recipient of this honor is a graduate with a 4.0 GPA who has completed the program of study within two years.
Leading the graduates as student marshals (all earning 4.0 GPAs) were:
* David Brian Craig of Linthicum, Associate of Applied Science degree, Medical Laboratory Technician;
* Ami Elisabet Dorn of Linthicum, Associate of Applied Science degree, Medical Assisting;
* Linda Howard Garrett of Severn, Associate of Applied Science degree, Hotel, Restaurant Management Culinary Arts Operations Option and an Associate of Arts degree, Theater Arts Option;
* Susan A. Koller of Millersville, Associate of Science degree, Nursing;
* Rachel M. Man of Pasadena, Associate of Arts degree, Transfer Studies;
* Barry Patrick McMenamin of Severna Park, Associate of Science degree, Engineering Transfer;
* Cheryl Lauren Schmidt of Linthicum, Associate of Science degree, Arts and Sciences Science and Premedical Professional Options, Nursing Option (prebaccalaureate nursing);
* Paula Berman Shantzis of Arnold, Associate of Applied Science degree, Architecture and Interior Design, Interior Design Option.
The valedictorian, presidential scholar and student marshals are selected through interviews with students who earned a 4.0 GPA and took at least 50 credits at AACC. Not every qualified student chose to compete for these honors.
Page 1 2


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Member Number on BP Doesn't Match Users Number on WP
We noticed on our site, http://www.NABCommunities.com that our members number is different from what WordPress tells us is our actual number of subscribers. Does anyone know why this is the case?
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Program Contacts
Attend an Information Session
Fall 2014 Recruiting Schedule
Program FAQs
Contact Us
How to Apply
Application Deadlines
Tuition and Financing
Admissions FAQs
Required Coursework
Summer Course Descriptions
Fall Course Descriptions
Spring Course Descriptions
Program Schedule
Steve Cohen, Program Director
Program Faculty
Alumni Profiles
Professional Development
Summer Workshop Projects
Fall Workshop Projects
Spring Workshop Projects
Forging Connections through ESP
Program alumni discuss their success after graduation
NYTimes: MPA Alum Takes on Mission to Separate Waste in NYC.
Hayley Rogers (ESP '11), makes an impact in the New York's recyling unit
Congratulations to the Class of 2014!
64 ESP Graduates Take Part in Columbia Commencement
Learn More About Our Program
Attend an Upcoming Information Session
Thinking About Sustainability
An Interview With Our Director
Recent News
MPA Alum Focuses on Energy Planning and Compliance
MPA Alum Serves as EDF Climate Fellow
MPA Students Discover the Gowanus Canal and the Lamont-Doherty Earth Observatory
MPA in Environmental Science and Policy Welcomes Class of 2015
From Our Director
Work, Youth, Optimism, and the Drive Toward a Safe, Sustainable Planet
Will de Blasio and Cuomo Make Sustainability a Higher Priority?
Our Dysfunctional National Government Is Incapable of Building a Sustainable Economy
NYC's Bike Sharing: The de Blasio Administration's First Expansion of the City's Sustainability Agenda
Information Sessions
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Incorporating a celebrity spokesperson in an advertising campaign is a powerful way to engage with your audience. When using a celebrity spokesperson it is important to position them in ways that will get attention. Coordinated Business Systems has featured Ron Gardenhire, the Minnesota Twins manager in their radio commercials going on 5 years. In 2010 and continuing in 2011 Ron and the President of Coordinated, Jim Oricchio have been highlighted in humor-oriented messages. The outcomes of have been:
1. More "talk" in the marketplace about Coordinated
2. Greater Brand Awareness for Coordinated
3. Faster recognition of the "Solutions" offered by Coordinated
Click the links below to listen to the Coordinated Business Systems Radio Commercials featuring Ron Gardenhire:
Document Management Radio Commercial
ProActive Managed IT Services Radio Commercial
To learn more about Orange Label's radio advertising services, contact us today.


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Town Square
SRVUSD Measure D research references
Original post made by CDSI community courtesy on Apr 30, 2008
CDSI provides the following research references for study of Measure D, the SRVUSD parcel tax measure on the June 2008 ballot:
Web Link
Web Link
Web Link
Contact your local school PTA legislation representative, or email firstname.lastname@example.com
Hal Bailey
CDSI Research Fellowship
Posted to Alliance of Community e-exchanges
on Apr 30, 2008 at 3:51 pm
Regarding Measure D, the school bond measure on the June ballot:
1. Don't reward what you don't want.
2. Punish tax and spend liberals.
Yes, they brag about good our schools do on tests. Well, duh, they should be excellent - after all, they're driving a Cadillac!
Put an end to limitless tax increases and pay raises.
on Apr 30, 2008 at 5:07 pm
Posted at the request of the author
Dear Ann,
Please provide specific objections and issues with Measure D. We are aware that Mr. Michael Arata and others plan to offer a detailed review of Measure D including their audits of sources and uses of funds.
This is a FORUM for discovery, commentary, consideration and consensus. Your neighbors welcome your concerns, studies and points of view.
Thank you for responding,
Kathy Bell
Iron Horse neighbors
Posted as received at firstname.lastname@example.com
on Apr 30, 2008 at 5:32 pm
Posted at the request of the author
Dear Ann and neighbors,
In our region, we would likely never find a liberal or anyone, at present, that believes in tax and spend. We are a community of moderates, well-educated, with an exceptional sense of humanity. We all understand the importance of education and we all value the cost of such education for ourselves and our children.
Schools and their districts do not run as businesses and educators are not fiscal professionals. It is reasonable that we, as owners of SRVUSD, provide fiscal review and planning. Let's focus on fully understanding the use of Measure D funds and how they specifically serve the education of our children.
Challenge SRVUSD to illustrate the fiscal results of Measure D and if satisfied vote YES in June.
Diablo Vista Region neighborhoods
Posted from Diablo Vista Region e-exchanges


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We're SorryClose
Terror-ific Kids' Costume Contest
Little Hench men
- Contest Photo by: Jackiee007
- La Puente, CA
- Category: Creative Creatures
Brief Description:
Kay is Shock, Luis is Lock
Red, puple and black felt. Tule brown paper bags, glue and paint.
Time to Create:
Contest Photo Tips and Guidelines:
- Avoid clutter. Keep the background simple -- your gingerbread house or holiday cookie is the star!
- Daytime, natural light is best. If your area is bright enough, try taking the photo without the flash.
- Upload a horizontal photo. If you crop the image on your computer, make sure you keep a landscape 4:3 aspect ratio.
- For best results, make your image 1200x900 (or "save for web").
- With a digital camera, you can take as many photos as possible and choose the best one. Have fun!


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Photo Gallery Day 3 Of UCI3
Posted on 11. Oct, 2010 by lyne in photos, race
Photo Gallery of Elite Women and Elite Men
See also:
Elite Women. Click for larger images or View [autoviewer id="61" width="1000" height="540" link="slideshow"]
Sue Butler (Hudz-Subaru) still at front ow chase group
Elite/U23 Men. Click for larger images or View [autoviewer id="62" width="1000" height="540" link="slideshow"]


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Midwestern University Glendale, Arizona tenure-track faculty positions
Faculty are expected to develop a productive research program with the potential to attract extramural funding. Departmental research strengths include: comparative and functional morphology of vertebrates, vertebrate paleontology, biological anthropology, and developmental and comparative neuroscience.
Applicants should submit a curriculum vita, the names of three references, and a statement describing research interests to:
Wade Grow, Ph.D., Chair of Anatomy
Midwestern University
19555 N. 59th Avenue
Glendale, AZ 85308
Qualified and interested applicants may apply online at www.midwestern.edu. Go to the 'Jump To' drop down box at the top of the page -> Employment at MWU
We are proud to be an EEO/AA employer M/F/D/V. We maintain a drug-free workplace and perform pre-employment substance abuse testing.


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My 50'000$ Honda has only 14 cupholders, while the 45'000$ Toyota of my friend has 22 cupholder. I like to talk to the Honda CEO, he needs to do something about that. Why does my Honda have fewer Cupholder than the cheaper Toyota.
I also need to bring my Honda to the dealer for inspection every 20'000km, while the Toyota only needs to go every 25'000km.
Honda will be going down if they continue like this. I will sell it and buy me a Toyota.
Wow... anyone ever stop to think that English may not be the OP's primary language? Who cares if the grammar isn't up to par. Many of the members of this forum make far less sense with their posts and English is often their primary Language. Give the OP a break. If you have something constructive to post then post it... otherwise stop insulting and move on to another thread...


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Anyone who has ever hit one of Tucson's many potholes has something riding on the outcome of the vote on Prop 409.
Proposition 409 will let the city float $100 million in bonds to fix city streets. Those opposed argue the taxpayer has already paid once and shouldn't have to again.
As of 12:30 a.m. Tuesday, the vote was too close to call. It was split 50 percent for and 50 percent against with 97 percent of precincts reporting. There are 900 more votes against the proposition.
Copyright 2012 Tucson News Now. All rights reserved.
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Exclusively from GoV
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Sturdy sterling silver ring with hearts. Learn More$12.99 $6.49
Purple Tiara RingWhile Supplies Last!
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Protector of Purity RingWhile Supplies Last!
"Love...always protects, always trusts, always hopes, always perseveres. Love never fails". 1 Corinthians 13: 7-8
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MADISON (WKOW) -- The Wisconsin Department of Agriculture, Trade and Consumer Protection urges Wisconsin residents to be aware of door-to-door contractors trying to help during flood clean up.
In the hardest hit areas, DATCP says some contractors will go door-to-door and promise to help fix any flooding problems at a great price. DATCP says these are "fly-by-night" contractors that will do work for high prices and they simply run off with a victim's money.
"Storm chasers travel to hard-hit communities and offer seemingly irresistible deals for construction or repair help," said Sandy Chalmers, Division Administrator for Trade and Consumer Protection.
"They pressure homeowners for up front payments or increase their prices arbitrarily as they work. These teams come and go from an area without a trace, leaving consumers empty-handed and with no recourse for any work they have not completed or any damage they've done to a homeowner's property," said Chalmers.
Consumer Protection has a few tips they recommend for homeowners:
1.) Be wary of any contractor who knocks at your door. Call the police or sheriff's department to check them out.
2.) Try to get a local contractor. Ask contractors if they are subcontracting your job. Be careful if local contractors are using outside subcontractors.
3.) Have someone watch the work being done. Check with your local building inspector to see if the work requires a permit and make sure an inspector visits the job site before you provide final payment.
4.) Request a copy of the contractor's certificate of liability insurance.
For more information from the DATCP, click here.


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Shakeout from Missouri River flooding includes predictable blame game
North America's communication towers rack up 7 million bird deaths a year, a new study reports
Why the myth of the robust western mountain dog is bunk
Advanced sonar helps pallid sturgeon biologists "see with sound"
A photographic peek at some busy western insects
Endangered American burying beetles may halt the Keystone XL pipeline
Incentive-based, voluntary conservation agreements help imperiled species in New Mexico
A tourist's urban misadventures on the Western Slope
A look at the Arizona monsoon season's faunal frenzy
A fond look at three unusual candidates for the Endangered Species Act
The West in your Inbox


text:
A collection of Lives columns from The New York Times.
The Unexpected 3 a.m. Guests
Two strangers were on the porch — and I opened the door.
August 31, 2014, Sunday
MORE ON LIVES AND: Crime and Criminals , Porches, Patios and Decks
Forgotten Postcards From Mexico City
My grandfather's letters reveal something of the man who never got home.
August 24, 2014, Sunday
MORE ON LIVES AND: Families and Family Life , Immigration and Emigration , Deportation , Communism (Theory and Philosophy) , Citizenship and Naturalization , Toys , Travel and Vacations
The Boy of Summer
I was 13 when a new friend joined me in the cornfield and in my home — and opened a door I hadn't known was closed.
August 17, 2014, Sunday
MORE ON LIVES AND: Homosexuality and Bisexuality
'The Driver Just Didn't See You'
A faceless bus driver left me feeling angry and alone. But I didn't really know him.
August 10, 2014, Sunday
MORE ON LIVES AND: Traffic Accidents and Safety , Buses , Pets , Concussions
How Ramen Got Me Through Adolescence
I had two sources of warmth during the cold middle-school years: a cocoon of books and a bowl of shelf-stable noodles.
August 3, 2014, Sunday
MORE ON LIVES AND: Noodles , Books and Literature , Teenagers and Adolescence , Children and Childhood , Cooking and Cookbooks , Education (K-12)
My Secret Pepsi Plot
After we emigrated to Brooklyn, my family was pinching pennies. At 10, I found a way to help.
July 27, 2014, Sunday
MORE ON LIVES AND: Parenting , Immigration and Emigration , Brooklyn (NYC) , Supermarkets and Grocery Stores
The Great Escape
Breaking free was a thrill, even if I didn't know what I was running from.
July 20, 2014, Sunday
MORE ON LIVES AND: Moral Majority , Camps and Camping , Religion and Belief , Hoaxes and Pranks , Christians and Christianity
My Mother's Miracle-Water Cure
When she arrived back home with a large plastic container, I knew there would be trouble.
July 13, 2014, Sunday
MORE ON LIVES AND: Hemophilia , Water , Alternative and Complementary Medicine
What Did My Mother the Chemist See in Betty Crocker?
It's hard to reconcile the all-American cookbook with the pragmatic, accomplished woman I know.
July 6, 2014, Sunday
MORE ON LIVES AND: Cooking and Cookbooks , Science and Technology
Looking for the Russia I Once Knew
Two decades later, the country was still inside me. But all around me, it had changed.
June 29, 2014, Sunday
MORE ON LIVES AND: Russia , Shoes and Boots
Rss Feeds On Lives
Subscribe to an RSS feed on this topic. What is RSS?


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A Summer in Katmai National Park
De Barrett Hedges
A Summer in Katmai National Park is a detailed photographic journal that follows Barrett's summer. From the moment he stepped off the float plane to the moment he got back on to leave is documented with pictures. Come follow Barrett as he experiences the bears of Katmai National Park.
Acerca del autor
I graduated from Carson-Newman College in May 2008, with a BA in Photography. Immediately after graduation I went to Denali National Park to work at a hotel for the summer. On my off days and weekends I hiked through much of the park. It was an incredible experience. The next summer I went to Katmai National Park, in Alaska, and was the tour bus driver to The Valley of Ten Thousand Smokes. Katmai has the largest population of protected brown bears of any national park, and I saw bears every day I was there. Working around these animals was an awesome experience. During the winters I have worked at Beaver Creek ski resort in Colorado. On off days and while skiing I will still enjoy the outdoors and go take pictures. Check out my website, www.bearheadphoto.com, to see my pictures.
Fecha de publicación 19 de abril de 2010
Dimensiones Vertical estándar 120 páginas
Papel estándar
Categoría Viajes
Etiquetas Valley of Ten Thousand Smokes, rainbow trout, sockeye salmon, Brown bears, Barrett, Katmai, cubs, waterfall, trout, salmon, travel, alaska, wildlife
michaelalvis dice
Beautiful photos, Barrett. Looking forward to seeing more of your Alaskan photographs this summer.
publicado 14 de jun. a las 19:39 PST
Conéctese o cree una cuenta para publicar un comentario.
Comience a crear su propio libro hoy desde 3.99 US $.
Sitios de Blurb


text:
WASHINGTON (AP) — Two of the loudest voices in the gun debate say it's up to voters now to make their position known to Congress.
New York Mayor Michael Bloomberg and National Rifle Associate Executive Vice President Wayne LaPierre claim their opposing views on guns have the support of the overwhelming number of Americans. They are looking at the next two weeks as critical to the debate, when lawmakers head home to hear from constituents ahead of next month's anticipated Senate vote on gun control.
Bloomberg, a former Republican-turned-independent, has just sunk $12 million for Mayors Against Illegal Guns to run television ads and phone banks in 13 states urging voters to tell their senators to pass legislation requiring universal background checks for gun buyers.
"We demanded a plan and then we demanded a vote. We've got the plan, we're going to get the vote. And now it's incumbent on us to make our voices heard," said Bloomberg.
Senate Majority Leader Harry Reid said Thursday that legislation would likely be debated in his chamber next month that will include expanded federal background checks, tougher laws and stiffer sentences for gun trafficking and increased school safety grants. A ban on assault-style weapons was dropped from the bill, fearing it would sink the broader bill. But Reid has said that he would allow the ban to be voted on separately as an amendment. President Barack Obama called for a vote on the assault weapons ban in his radio and Internet address Saturday.
Recalling the horrific shooting three months ago at a Newtown, Conn., elementary school that left 20 first-graders and six school administrators dead, Bloomberg said it would be a great tragedy if Congress, through inaction, lost the moment to make the country safer from gun violence. Bloomberg said that 90 percent of Americans and 80 percent of NRA members support universal background checks for gun purchases.


text:
The leader of a Syrian-based radical Palestinian group is planning to
assassinate Palestinian Authority President Mahmoud Abbas, Fatah claimed on
The claim followed statements made by Ahmed Jibril, leader of
the Popular Front for the Liberation of Palestine, General Command, in which he
strongly denounced Abbas and his predecessor, Yasser Arafat, for signing the
Oslo Accords with Israel.
"Ahmed Jibril is a hired agent," Fatah said in
a statement released in Ramallah. "He is operating on instructions from his
handlers in the region who seek to eliminate President Abbas."
statement did not name the "handlers."
However, a Fatah official in
Ramallah told The Jerusalem Post that the statement referred to Syria and
According to Fatah, Jibril's attacks on the PA leadership came
after he "failed to exploit 'Land Day' to achieve political gains."
accused Jibril of planning to dispatch Palestinians to the borders of Israel as
part of the Global March to Jerusalem, an event that was organized last Friday
by a coalition of pro-Palestinian organizations around the world.
Jibril is a merchant for Palestinian and Arab blood," Fatah charged. "He is
desperately trying to appease outside parties in an attempt to destroy the
political achievements of our people and their wise leadership."
belongs to a group of Syrian-based terror organizations that are strongly
opposed to peace with Israel.
His close ties with the Syrian authorities
have won him many enemies among the Palestinians, especially the PA
The PA recently accused Jibril's supporters of killing 14
Palestinians in a refugee camp near the Syrian capital of Damascus.


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STUDENT Login
Vice-Chancellor Message
Education is transmission of Civilization and its globalization has made it more quality and competition oriented. We intend to move towards a better tomorrow by following best practices of imparting education to match the international standards. University intends to implement industry friendly, demand driven, research oriented
Dr.Ashish Dongre
(Vice-Chancellor , RKDF UNIVERSITY, Bhopal ) Read More+


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2 bed, 1 full bath, 1 half bath. Rare on the market ,Art Deco Townhouse right off Lincoln road pedestrian street & close to EspanolaMore info
3 bed, 2 full bath, 1 half bath. LAKEFRONT! BRIGHT & SPACIOUS 2-STORY TOWNHOME IN SOUGHT OF AFTER COMMUNITY, "SAILPOINTE".. ENJOY PLMore info
2 bed, 2 full bath. great views from this large south corner unit. this unit has all ceramic floors, open remodeled kitMore info
1 bed, 1 full bath, 1 half bath. Beautiful and completely remodeled. Granite countertops. Tiles throughout the condo. From the balcoMore info
3 bed, 2 full bath, 1 half bath. Bright and spacious Unit in the Most Desired Community of the area. Located on the second floor. ThMore info
3 bed, 2 full bath. NOT A SHORT SALE NOR A REO. DONT LOOK NO MORE ONE OF THE BEST OR IS THE BEST TOWN HOME AVAIBLE FORMore info
1 bed, 1 full bath. COME ENJOY THE LUXURY OF A 5 STAR RESORT IN YOUR VERY OWN HOME. FROM WHITE PORCELAIN FLOORS, STAINLMore info
4 bed, 3 full bath, 1 half bath. BEAUTIFUL TOWHNSE IN A GREAT LOCATION IN THE CITY OF DORAL. GREAT LAYOUT and Kitchen with brand newMore info
, 1 full bath. Largest floor plan for this 760 sfeet studio in famous Roney palace. this unit has a renovated EuroMore info
2 bed, 2 full bath, 1 half bath. Incredible oppty steps from the Ocean! New Const. 2005,spacious 2bed,2. 5 bath townhome. Flooded wiMore info


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Town Square
Palo Alto man arrested for burglary, fighting
Original post made on Aug 11, 2007
Read the full story here Web Link posted Friday, August 10, 2007, 4:16 PM
on Aug 11, 2007 at 5:11 pm
always remember when reading police ''reports''. not everyone who is charged with a particular offense actually did what police say. if you are african american or latino , a white person can accuse you of something even after they may have assaulted YOU!! police in palo alto cannot be trusted!!
on Aug 12, 2007 at 11:09 am
The behavior displayed by the Oppportunity Center resident has nothing to do with his race or with misbehavior on the part of the police. It has everything to do with his own bad behavior, which in turn provides another valid reason for requiring drug testing at the homeless center. The big question now is what those in charge of the Opportunity Center are going to do about it?? Will Miller be allowed to just rejoin the homeless community there at the Opportunity Center or is better behavior required in order to maintain your place at the center? This is a good test to determine whether we have responsible adults at the Center or just adults who make excuses for bad behavior. Another question I'd like to have answered is whether Miller is a long time resident of Palo Alto or whether he was attracted to our community by the presence of the homeless shelter?
on Aug 12, 2007 at 11:19 am
wow this guy punched through a window? that kicks ass!


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Preview | Recap | Results (Saint Mary's Invitational - 36 Holes) | Results (Saint Mary's Invitational - Final RD)
@FGCU_MGolf Returns To Action Monday and Tuesday At The Saint Mary's Invitational
SEASIDE, Calif. – Coming off an 11th-place finish at the competitive Golfweek Conference Challenge in Iowa last week, the FGCU men's golf team returns to action Monday and Tuesday in California as part of the Saint Mary's Invitational at Bayonet & Black Horse Golf Course.
The tournament features a 54-hole format with the low four scores from each team's designated five-player lineup counting toward the team total each round. The teams will play 36 holes on Monday, followed by a final round of 18 holes on Tuesday. Each day of competition will begin with an 8 a.m. shotgun start. Live tournament coverage will be available via Golfstat, with scoring updates every three holes.
The 19-team tournament field includes squads from the nine different conferences, including the Atlantic Coast, Big 12, Big East, Big West, Mid-America, Mountain West, Pac-12 and West Coast conferences.
In addition to the host Gaels and FGCU, the field includes Akron, Ball State, California, CSU Northridge, Fresno State, Hawai'i, Kansas State, Long Beach State, Loyola Marymount, North Carolina State, Oregon, St. John's, San Diego, San Francisco, Santa Clara, UC Irvine and UC Riverside.
The event will serve as a homecoming for FGCU head coach Brent Jensen, who graduated from Saint Mary's in 2004. This will mark the fourth consecutive season the Green and Blue has returned to the West Coast, and the team is coming off a 15th-place finish last year.
The field is not as competitive as in FGCU's first tournament of the season at the Golfweek Conference Challenge, but still boasts 10 of the top-100 teams in the nation, six of the top 65 and No. 1 California (based off the most recent Golfweek rankings from June).
Bayonet Golf Course, designed in 1954, was recently redesigned to offer expanded views of the Monterey Bay. The overall length of Bayonet increased only 16 yards from its original design, but still measures 7,104 yards with oak and cypress-lined fairways, has four sets of tees and is a par-72 layout.
Black Horse Golf Course was designed in 1964, and includes breath-taking views of the bay. The most recent renovations transformed Black Horse GC into a longer, more challenging championship golf course. With long rolling fairways and panoramic views of the Monterey Bay, from the back tees Black Horse is a par-72 layout, measuring 7,024 yards, with a slope of 141 and a rating of 73.7.
FGCU is coming off the aforementioned 11th-place finish in Burlington, Iowa, at the Golfweek Conference Challenge last Sunday, Monday and Tuesday. Junior transfer Blake Biggs (Peoria, Ill./Eastern Florida State College/Peoria Notre Dame HS) shot a team-low 221, including a 74 in the closing round. Biggs led FGCU in two of the three rounds, with defending Atlantic Sun Conference Freshman of the Year Edward Figueroa (Naples, Fla./St. John Neumann HS) carding the low round (72) on Monday.
Following the Saint Mary's Invitational, the Eagles don't return to the course for competitive action again until Oct. 21-22 in Atlanta, Ga., for the Pinetree Intercollegiate hosted by Kennesaw State.
Tournament Field (GolfWeek.com Rankings From Last Week Of 2012-13 Season)
#1 California
#30 Saint Mary's
#44 Oregon
#54 NC State
#57 San Francisco
#61 Ball State
#71 San Diego
#82 UC Irvine
#84 Fresno State
#86 Long Beach State
#105 Kansas State
#123 Akron
#135 Loyola Marymount
#139 St. John's
#148 Santa Clara
#157 Hawai'i
#186 UC Riverside
#224 CSU Northridge
|Copyright ©2014 FGCU Athletics. All Rights Reserved.


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Trioscapes have released their sophomore album worldwide on Metal Blade Records! After the album's first week of release, it has landed on the Billboard Current Traditional Jazz albums chart at #4.Read More
Don Jamieson
Hookers and Blow is excited to add longtime friend and world renowned stand-up comic DON JAMIESON (VH1's THAT METAL SHOW) as the 'Master of Ceremonies' for their upcoming East Coast dates.Read More
Last week saw the release of the new album 'Maximum Overload' from DragonForce. Today the album has debuted on multiple Billboard Charts, including 62 on the Billboard Top 200 Chart.Read More
Anaal Nathrakh
Birmingham extreme metal miscreants and recent Metal Blade signees, ANAAL NATHRAKH, are pleased to infect the masses with their anticipated new full-length this Fall title 'Desideratum'.Read More
Cannibal Corpse
In addition to releasing their thirteenth full-length album, 'A Skeletal Domain,' the death metal icons will also be releasing their official autobiography on September 16th, 'Bible of Butchery The Official Biography'Read More
Between the Buried and Me
Progressive powerhouse Between the Buried and Me will be releasing 'Future Sequence: Live at the Fidelitorium' next month on Metal Blade Records.Read More
New Releases
Metal Blade TV
Upcoming Releases
Latest News
Rivers of Nihil joins Death (DTA Tours) "Swamp Leper Stomp '14″ Tour
- August 5th, 2014 – This November, Pennsylvania's Rivers of...Read More
Trioscapes launches "Digital Dream Sequence" studio video
- August 4th, 2014 – Progressive/fusion trio, Trioscapes, will release...Read More
Wovenwar: stream their entire debut album online now!
- August 1st, 2014 – Wovenwar is streaming their debut self-titled...Read More
Below release brand new video for "Bid You Farewell" on InvisibleOranges.com
- July 31st, 2014 – Swedish doom act, Below, have released a new video...Read More
Theories signs with Metal Blade Records
- July 31st, 2014 – Seattle, WA based death/grind newcomers, Theories,...Read More
God Dethroned plot their return to the stage for 2015
- July 30th, 2014 – Influential Dutch death metal stalwarts God...Read More
Antropomorphia armed and ready to release new album 'Rites Ov Perversion'!
- July 30th, 2014 – New song and pre-orders available now! Dutch...Read More
Brimstone Coven: New Cosmic Hymn From Retro/Doom Metal Sorcerers Now Playing At Revolver
- July 30th, 2014 – Release Day Draws Near As retro doom metal...Read More


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Martin Short
Here's a look at Martin Short over the years.
LI people on the move! Promotions, new jobs Westhampton football practice Long Island and New York City's top photos Floral Park football practice What's your favorite beer? 68 LIers share theirs' LI car accidents Meet Long Island's pageant queens LI notable deaths 90 celebrities remember life on Long Island Liv Tyler, HBO co-stars visit Malverne Mepham football practice Billy Joel now and then