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New mass spectrometry based methodology to sequence a whole snake venom
Echterbille, Julien ; ; Degueldre, Michel et al
Poster (2014, February 10)
Animal venoms are complex chemical cocktails, comprising wide ranges of biologically active reticulated peptides that target with high selectivity and efficacy varieties of membrane receptors. Assuming ... [more ▼]
Animal venoms are complex chemical cocktails, comprising wide ranges of biologically active reticulated peptides that target with high selectivity and efficacy varieties of membrane receptors. Assuming the fact that each of the 170,000 venomous species reported can produce more than 250 bioactive toxins, at least 40,000,000 bioactive peptides and proteins may be discovered. Among the four described species of mambas, Eastern Jameson's mamba (Dendroaspis jamesonii kaimosae) venom is the less characterized since only 9 peptides are referenced in database. This work aims at developing a new strategy devoted to the deep analysis of animal venoms. Our approach consists in a first separation of the venom using cation exchange chromatography. Each primary fraction is then purified a second time by classical RP-HPLC. A total of 328 fractions, containing amongst 1 and 4 toxins, are finally collected. MALDI-MS analysis of each fraction is done in order (1) to obtain information about masses and (2) to obtain sequences of toxins thanks to MALDI-In Source Decay (ISD) dissociation coupled with on MALDI target plate reduction of the peptides. ISD has already been demonstrated efficient for toxin sequencing, and especially when using 1,5-DAN as reducing matrix. ISD yields to sequences that cover more than 50% of peptide sequences by series of singly charged c-type ions. Thanks to this methodology, we were able to obtain 85% of satisfactory results i.e. spectra giving quite long tags of amino acids (up to 20 residues). As a way to validate our method, a tag coming from ISD spectrum interpretation has found a match in database for an Eastern Jameson's mamba toxin. The global sequence has then been obtained by extrapolation on the ISD spectrum. Since ISD spectra are simpler than classical MS/MS spectra, automation of spectra interpretation, difficult with other fragmentation techniques (CID, ETD…), is implementable. In the near future, sequences obtained with this approach will be used to direct tests of biological activity through sequence homologies with already known ligands for different kinds of membrane receptors. [less ▲]Detailed reference viewed: 43 (7 ULg)
Combined use of Ion Mobility and Collision-Induced Dissociation (CID) to investigate the reactivity of disulfide-bridged peptides in Electron Transfer Dissociation (ETD)
Massonnet, Philippe ; ; et al
Poster (2014, January)Detailed reference viewed: 23 (8 ULg)
Advances in proteomics for the FP7 Venomics project - Disulfide bridge assignement task
Massonnet, Philippe ; ; et al
Scientific conference (2013, December 18)Detailed reference viewed: 24 (6 ULg)
Attribution of Cysteine Connectivities in small toxins - New Prospects Based on Partial Oxidation/Reduction Experiments and Ion-Mobility Mass Spectrometry
Quinton, Loïc ; Massonnet, Philippe ; Echterbille, Julien et al
Conference (2013, December)
Disulfide bonds are post-translational modifications often found in biological compounds and especially in animal toxins. Disulfide bonds participate in the formation of specific folding of peptides and ... [more ▼]
Disulfide bonds are post-translational modifications often found in biological compounds and especially in animal toxins. Disulfide bonds participate in the formation of specific folding of peptides and proteins, directly related to their biological activity. Cystein pairing determinations are primordial for the synthesis of chemical homologous displaying the same bioactivity than the natural compound. This task appears already difficult when the cysteine pairings have to be determined from large proteins. The combination of physical and chemical techniques such as NMR, enzymatic proteolysis, liquid chromatography and mass spectrometry, is needed to circumvent this difficulty. However, when the work concerns small compounds such as conotoxins, the problem is much more complex due to the low amount of available compound and to the lack of enzymatic cleavage sites between cysteines. In this study, we investigate the case of small peptides that contain two disulphide bonds. The idea is to determine the cystein pairings in such compounds by a chemical partial reduction (or oxidation) of the peptides, followed by the separation of the generated species by ion-mobility mass spectrometry, and their characterisation by tandem mass spectrometry. Up to now, we have investigated the partial reduction not only in solution (with DTT and TCEP) but also in the gas-phase (Electron transfer dissociation), and partial oxidation in solution (with 3-CPBA). The results demonstrate an unexpected complexity of the data, including low fragmentation ratios of peptides and disulfide scramblings. [less ▲]Detailed reference viewed: 24 (10 ULg)
Peptidomic comparison and characterization of the major components of the venom of the giant ant Dinoponera quadriceps collected in four different areas of Brazil.
; ; et al
in Journal of Proteomics (2013), 94
Despite the noxious effects inflicted by Dinoponera ant's envenomation, the information about the biological properties and composition of their venom is still very limited. Ants from the genus Dinoponera ... [more ▼]
Despite the noxious effects inflicted by Dinoponera ant's envenomation, the information about the biological properties and composition of their venom is still very limited. Ants from the genus Dinoponera are believed to be the world's largest living ants with a body length of 3cm. Their occurrence is restricted to tropical areas of South America. In this work, we study the venom of the giant Dinoponera quadriceps ant collected in 4 different regions of Brazil. By using a combination of complementary mass spectrometric approaches, we aim at: (i) characterizing the venom composition of these ants; (ii) establishing a comparative analysis of the venom from four geographically different regions in Brazil. This approach demonstrates that ant venom is a copious source of new compounds. Several peptides were identified and selected for "de novo sequencing". Since most of the new peptides showed similarities with antimicrobial peptides (AMPs), antimicrobial assays were performed with the purpose of evaluating their activity. In regard to the comparative study of the four regions, we observed not only major differences in the venom compositions, but also that the venoms collected in closest areas are more similar than the ones collected in distant regions. These observations seem to highlight an adaption of the ant venoms to the local environment. Concerning the biological assays, the peptides called Dq-3162 and Da-3177 showed a wide-ranging antimicrobial activity. The characterization of new AMPs with a broad spectrum of activity and different scaffolds may aid scientists to design new therapeutic agents and understand the mechanisms of those peptides to interact with microbial membranes. The results obtained betoken the biotechnological potential of ant's venom. BIOLOGICAL SIGNIFICANCE: For the first time this manuscript describes an extensive proteomics characterization of the D. quadriceps venom. In addition this study reports the variation in venom composition of primitive ants from 4 geographically different areas of Brazil. The results reveal the presence of ~335 compounds for each venom/area and inter-colony variations were observed. 16 new peptides were characterized and 2 of them were synthesized and biologically assayed. These findings highlight the considerable and still unexplored diversity of ant's venom which could be used as valuable research tools in different areas of knowledge. [less ▲]Detailed reference viewed: 19 (2 ULg)
Combined use of mass spectrometry techniques for cysteine connectivity identification for peptides bearing 2 disulfide bonds
Massonnet, Philippe ; Echterbille, Julien ; et al
Conference (2013, November 15)Detailed reference viewed: 25 (5 ULg)
Structural characterization of disulfide-bridged-peptides by a combined use of ETD, CID and Ion-Mobility mass spectrometry
Massonnet, Philippe ; ; et al
Conference (2013, September 05)Detailed reference viewed: 14 (4 ULg)
Use of 1,5-diaminonaphthalene to combine matrix-assisted laser desorption/ionization in-source decay fragmentation with hydrogen/deuterium exchange
; Debois, Delphine ; Smargiasso, Nicolas et al
in Rapid Communications in Mass Spectrometry [=RCM] (2013), 27(16), 1837-1846
In-Source Decay (ISD) in Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometry is a fast and easy top-down activation method. Our objective is to find a suitable matrix to locate the ... [more ▼]
In-Source Decay (ISD) in Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometry is a fast and easy top-down activation method. Our objective is to find a suitable matrix to locate the deuterons following in-solution hydrogen/deuterium exchange (HDX). This matrix must circumvent the commonly encountered undesired back-exchange reactions, in order to preserve the regioselective deuteration pattern. The 1,5-diaminonaphthalene (1,5-DAN) matrix is known to be suitable for MALDI-ISD fragmentation. MALDI Mass Spectrometry Imaging (MSI) was employed to compare 1,5-DAN and other commonly used MALDI matrices with respect to the extent of back-exchange and the uniformity of the H/D exchange profiles within the MALDI spots. We tested the back-exchange on the highly sensitive amyloid-beta peptide (1-40), and proved the regioselectivity on ubiquitin and b-endorphin. MALDI-MSI results show that 1,5-DAN leads to the least back-exchange over all the spot. MALDI-ISD fragmentation combined with H/D exchange using 1,5-DAN matrix was validated by localizing deuterons in native ubiquitin. Results agree with previous data obtained by Nuclear Magnetic Resonance (NMR) and Electron Transfer Dissociation (ETD). Moreover, 1,5-DAN matrix was used to study the H/D exchange profile of the methanol-induced helical structure of b-endorphin, and the relative protection can be explained by the polarity of residues involved in hydrogen bond formation. We found that controlling crystallization is the most important parameter when combining H/D exchange with MALDI. The 1,5-DAN matrix is characterized by a fast crystallization kinetics, and therefore gives robust and reliable H/D exchange profiles using MALDI-ISD. [less ▲]Detailed reference viewed: 20 (3 ULg)
Structural characterization of disulfide-bridged-peptides by a combined use of ETD and Ion-Mobility mass spectrometry
Massonnet, Philippe ; Quinton, Loïc ; Smargiasso, Nicolas et al
Poster (2013, June 11)Detailed reference viewed: 56 (19 ULg)
De novo sequencing of unusual non tryptic peptides thanks to 4-sulfophenylisothiocyanate derivatization by post-source decay MALDI-MS.
Echterbille, Julien ; Quinton, Loïc ; et al
Poster (2013, June 11)
Introduction Due to the specificity of trypsin, tryptic peptides contain basic residues on the C-terminal side. This feature provides good ionization efficiency, and facilitates fragmentation processes ... [more ▼]
Introduction Due to the specificity of trypsin, tryptic peptides contain basic residues on the C-terminal side. This feature provides good ionization efficiency, and facilitates fragmentation processes. In the case of non tryptic peptides, the absence of basic residues at one extremity implicates lower fragmentation ratio and poor MS/MS spectra. Several methods have been developed to circumvent this drawback. Derivatization of peptides with compounds containing positive charge has been studied; Chen et al. (RCMS, 2004, 18, 191) demonstrated the simplification of CID spectra of tryptic peptides modified by 4-sulfophenylisothiocyanate. The result is a predominance of y-type ions. In this work, we evaluate the potential of SPITC for the de novo sequencing of unknown non-tryptic peptides containing disulfide bridges, i.e. peptide toxins from animal venoms. Methods 2µL of peptide solution (100 µM) were diluted in 6µL NH4HCO3 50mM (pH 8.7). As peptide toxins often contain disulfide bridges, reduction (2µL DTT 50mM, 1h at 56°C) and alkylation (2µL IAA 500mM, 1h in darkness at RT) of peptides were performed before the derivatization reaction. Peptides were then adsorbed on a C18 ZipTip micro-column followed by 10 µL of 4-sulfophenylisothiocyanate (SPITC) 50mM. The column was then incubated for 6h at 56°C. Peptides were washed by TFA 0.2% and eluted in 10µL 50/50 ACN/FA 0.1%, before being spotted in 2,5-DHB. MS experiments were performed using a Bruker Ultraflex II MALDI-TOF/TOF. FlexControl 3.0, FlexAnalysis 3.0, BioTools 3.2 and SequenceEditor 3.2 softwares (Bruker Daltonics, Bremen) were used for data acquisition and interpretation. Preliminary data According to our first results, SPITC derivatization allows in positive mode to direct the fragmentation thanks to the acidic character of the sulfonate moiety present on the modified molecule. Indeed, a large series of y-type ions is found in the CID spectra allowing determining easily large sequence tags. Moreover, the number of C-terminus ions (b- and a-type ions) decreases, which improve the simplification of MS/MS spectra. Due to this fragmentation pattern, SPITC derivatization is clearly valuable for the sequencing of peptides that are not described in databases (de novo sequencing). For example, animal venoms are composed of several hundreds of peptides that are poorly studied, up to now. These peptides display a high importance for pharmaceutical applications and their sequencing is, as a consequence, of prime interest. Peptide toxins, which are not resulting from an enzymatic digestion, are however difficult to sequence by classical MS/MS methods. In this work, we demonstrate that the modification of peptide toxins with SPITC reagent is suitable for "real" de novo sequencing. The method was applied to isolated peptides as well as chromatographic fractions that contain up to 30 toxins. The perspectives of this work rest on the study of the SPITC modified peptides in negative mode. We expect to obtain a better sensitivity due to the presence of the negative sulfonic acid group at the N-terminus extremity, and also interesting MS/MS spectra including mainly a- or b-type ions. The final challenge will be the application of the protocol to high throughput sequencing of peptide toxins from a large variety of animal venoms. Novel aspect De novo sequencing of unusual non-tryptic peptides thanks to 4-sulfophenylisothiocyanate derivatization by post-source decay MALDI-MS [less ▲]Detailed reference viewed: 58 (12 ULg)
ISD high-throughput platform for the sequencing of animal toxins
Degueldre, Michel ; Quinton, Loïc ; Smargiasso, Nicolas et al
Poster (2013, June)Detailed reference viewed: 68 (14 ULg)
Les venins animaux sont-ils les pharmaciens du futur?
Quinton, Loïc
in Chimie Nouvelle (2013)Detailed reference viewed: 26 (5 ULg)
Structural characterization of disulfide-bridged-peptides by a combined use of ETD and Ion-Mobility mass spectrometry
Massonnet, Philippe ; Quinton, Loïc ; Smargiasso, Nicolas et al
Poster (2013, May 03)Detailed reference viewed: 55 (34 ULg)
Les venins d'animaux, nouvelle panacée?
Echterbille, Julien ; Quinton, Loïc ; De Pauw, Edwin
in Athena (2013)
Araignées, serpents, scorpions,… autant d'animaux ayant une place particulière dans l'imaginaire collectif. Fascinants, horripilants voire même terrifiants, les adjectifs ne manquent pas pour qualifier ... [more ▼]
Araignées, serpents, scorpions,… autant d'animaux ayant une place particulière dans l'imaginaire collectif. Fascinants, horripilants voire même terrifiants, les adjectifs ne manquent pas pour qualifier les réactions qu'ils suscitent auprès des populations. Que dire alors de la peur engendrée par leur venin. Une simple piqûre, morsure ou contact peut s'avérer extrêmement dangereux voire létal... [less ▲]Detailed reference viewed: 47 (22 ULg)
Advances in proteomics for the FP7 Venomics project
Degueldre, Michel ; Quinton, Loïc ; De Pauw, Edwin
Scientific conference (2013, April)Detailed reference viewed: 21 (6 ULg)
BcsTx3 is a founder of a novel sea anemone toxin family of potassium channel blocker
; ; et al
in FEBS Journal (2013), 280
Sea anemone venoms have become a rich source of peptide toxins which are invaluable tools for studying the structure and functions of ion channels. In this work, BcsTx3, a toxin found in the venom of a ... [more ▼]
Sea anemone venoms have become a rich source of peptide toxins which are invaluable tools for studying the structure and functions of ion channels. In this work, BcsTx3, a toxin found in the venom of a Bunodosoma caissarum (population captured at the Saint Peter and Saint Paul Archipelago, Brazil) was purified and biochemically and pharmacologically characterized. The pharmacological effects were studied on 12 different subtypes of voltage-gated potassium channels (KV1.1–KV1.6; KV2.1; KV3.1; KV4.2; KV4.3; hERG and Shaker IR) and three cloned voltagegated sodium channel isoforms (NaV1.2, NaV1.4 and BgNaV1.1) expressed in Xenopus laevis oocytes. BcsTx3 shows a high affinity for Drosophila Shaker IR channels over rKv1.2, hKv1.3 and rKv1.6, and is not active on NaV channels. Biochemical characterization reveals that BcsTx3 is a 50 amino acid peptide crosslinked by four disulfide bridges, and sequence comparison allowed BcsTx3 to be classified as a novel type of sea anemone toxin acting on KV channels. Moreover, putative toxins homologous to BcsTx3 from two additional actiniarian species suggest an ancient origin of this newly discovered toxin family. [less ▲]Detailed reference viewed: 6 (2 ULg)
MALDI In-Source Decay, from sequencing to imaging
Debois, Delphine ; Smargiasso, Nicolas ; Demeure, Kevin et al
in Topics in Current Chemistry (2013), 331
MALDI is now a mature method allowing the identification and, more challenging, the quantification of biopolymers (proteins, nucleic acids, glycans…). MALDI spectra show mostly intact singly charged ions ... [more ▼]
MALDI is now a mature method allowing the identification and, more challenging, the quantification of biopolymers (proteins, nucleic acids, glycans…). MALDI spectra show mostly intact singly charged ions. To obtain fragments, the activation of singly charged precursors is necessary, but not efficient above 3.5 kDa thus making MALDI MS/MS difficult for large species. In-source decay (ISD) is a prompt fragmentation reaction that can be induced thermally or by radicals. As fragments are formed in the source, precursor ions cannot be selected; however, the technique is not limited by the mass of the analyzed compounds and pseudo MS/MS can be performed on intense fragments. The discovery of new matrices that enhance the ISD yield, combined with the high sensitivity of MALDI mass spectrometers, and software development, opens new perspectives. We first review the mechanisms involved in the ISD processes, then discuss ISD applications like top-down sequencing and post-translational modifications studies, and finally review MALDI-ISD tissue imaging applications. [less ▲]Detailed reference viewed: 98 (38 ULg)
Expression of a protease of biotechnological interest cloned from C. d. collilineatus venom gland
; ; et al
Poster (2013)Detailed reference viewed: 14 (0 ULg)
Peptide backbone fragmentation initiated by side-chain loss at cysteine residue in matrixassisted laser desorption/ionization in-source decay mass spectrometry
; Smargiasso, Nicolas ; Quinton, Loïc et al
in Journal of Mass Spectrometry [=JMS] (2013), 48
Matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) is initiated by hydrogen transfer from matrix molecules to the carbonyl oxygen of peptide backbone with subsequent radical-induced ... [more ▼]
Matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) is initiated by hydrogen transfer from matrix molecules to the carbonyl oxygen of peptide backbone with subsequent radical-induced cleavage leading to c0/z• fragments pair. MALDI-ISD is a very powerful method to obtain long sequence tags from proteins or to do de novo sequencing of peptides. Besides classical fragmentation, MALDI-ISD also shows specific fragments for which the mechanism of formation enlightened the MALDI-ISD process. In this study, the MALDI-ISD mechanism is reviewed, and a specific mechanism is studied in details: the N-terminal side of Cys residue (Xxx-Cys) is described to promote the generation of c0 and w fragments in MALDI-ISD. Our data suggest that for sequences containing Xxx-Cys motifs, the N–Ca bond cleavage occurs following the hydrogen attachment to the thiol group of Cys side-chain. The c•/w fragments pair is formed by side-chain loss of the Cys residue with subsequent radical-induced cleavage at the N–Ca bond located at the left side (N-terminal direction) of the Cys residue. This fragmentation pathway preferentially occurs at free Cys residue and is suppressedwhen the cysteines are involved in disulfide bonds. Hydrogen attachment to alkylated Cys residues using iodoacetamide gives free Cys residue by the loss of •CH2CONH2 radical. The presence of alkylated Cys residue also suppress the formation of c•/w fragments pair via the (Cb)-centered radical, whereas w fragment is still observed as intense signal. In this case, the z• fragment formed by hydrogen attachment of carbonyl oxygen followed side-chain loss at alkylated Cys leads to a w fragment. Hydrogen attachment on peptide backbone and side-chain of Cys residue occurs therefore competitively during MALDI-ISD process. [less ▲]Detailed reference viewed: 12 (6 ULg)
Identification and functional characterization of a novel αlpha-conotoxin (EIIA) from Conus ermineus
Quinton, Loïc ; ; et al
in Analytical and Bioanalytical Chemistry (2013), 405
Nicotinic acetylcholine receptors (nAChRs) are one of the most important families in the ligand-gated ion channel superfamily due to their involvement in primordial brain functions and in several ... [more ▼]
Nicotinic acetylcholine receptors (nAChRs) are one of the most important families in the ligand-gated ion channel superfamily due to their involvement in primordial brain functions and in several neurodegenerative pathologies. The discovery of new ligands which can bind with high affinity and selectivity to nAChR subtypes is of prime interest in order to study these receptors and to potentially discover new drugs for treating various pathologies. Predatory cone snails of the genus Conus hunt their prey using venoms containing a large number of small, highly structured peptides called conotoxins. Conotoxins are classified in different structural families and target a large panel of receptors and ion channels. Interestingly, nAChRs represent the only subgroup for which Conus has developed seven distinct families of conotoxins. Conus venoms have thus received much attention as they could represent a potential source of selective ligands of nAChR subtypes. We describe the mass spectrometric based approaches which led to the discovery of a novel α-conotoxin targeting muscular nAChR from the venom of Conus ermineus. The presence of several posttranslational modifications complicated the N-terminal sequencing. To discriminate between the different possible sequences, analogs with variable N-terminus were synthesized and fragmented by MS/MS. Understanding the fragmentation pathways in the low m/z range appeared crucial to determine the right sequence. The biological activity of this novel α-conotoxin (α-EIIA) that belongs to the unusual α4/4 subfamily was determined by binding experiments. The results revealed not only its selectivity for the muscular nAChR, but also a clear discrimination between the two binding sites described for this receptor. [less ▲]Detailed reference viewed: 10 (2 ULg)


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Ex-nuclear regulatory commission chief says no U.S. nuclear plants are safe, while California's San Onofre plant plans to restart
As his retirement looms, Sen. Jeff Bingaman, D-N.M., pushes a "clean energy" bill, one that broadens the energy mix beyond traditional "renewable" sources.
The House of Representatives passes an energy bill with even more industrial pork than the Bush administration requested.
The French have dealt with their radioactive waste for
decades by reprocessing it, but the process is more problematic
than it sounds, particularly in an age of terrorism
High Country News reveals its odd
historical connection with the West's uranium obsession of
the 1950s
The uranium mill proposed for western Colorado will have harmful effects on the health and environment of the entire region.
Even as the federal government pushes for more nuclear
power plants and nuclear weapons, cleanup lags far behind on the
West's most contaminated nuclear sites.
Uranium mining hasn't caused any massive cave-ins or oil spills, but it has resulted in a huge death toll all the same.
Westerners have a knack for new and innovative thinking, such as: Redefining rancher politics, A rediscovered renewable, Creating public nooks and crannies and more.
Jane Goetze says that before the U.S. embraces nuclear
power again, we should finish cleaning up the last round of
mistakes, remembering how many uranium miners have already
The West in your Inbox

subset: commoncrawl


text:
Your old class ring or that set of silver missing a few pieces could make you money.
The Great American Treasure Hunt is set up this week in the Walnut Room of the Holiday Inn Joplin. Times are 9 a.m. to 6 p.m. today through Friday, then 9 a.m. to 4 p.m. on Saturday.
Old jewelry is considered a find by the treasure hunt buyers who paid a thousand dollars for a carat diamond.
While they're not appraisers they can determine values of gold and silver coins and jewelry.
Buyers say people may have things in their attic of value to collectors, like a flag from World War II and photos from a war photographer.
"The main thing is getting people out here to see what they have," says Janice Hall, Manager of the Great American Treasure Hunt. "The average person does not know the actual value of something - that's what we're here for. It's a free event and they get cash on the spot for their items, so it's really a good thing and it helps the economy here because they're going to spend that money right here in Joplin or wherever they live at."


text:
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text: As I am sure you all are aware, even during the regular season, the readership here at The 7th Floor can be a bit sporadic. One thing I learned over at EDSBS is that a strong twitter following of the commenters can help bring together a site's readers, and enable it to grow pretty well. So I am proposing that if you are a regular, or even a random/first time reader of our humble little blog, go ahead and post your Twitter handle in the comments below. The more we get our fans to interact, the better it will be for the blog. If you want to follow any of us, but don't wish to give out your handle, thats perfectly fine as well. Mine is in my sig, but for those who dont like clicking links, it is @JdotLeazy.


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BankUnited directors Chinh E.Chu, Pierre Olivier Sarkozy, Wilbur L. Ross Jr., and Lance N. West have separately notified BankUnited's board that they will not stand for re-election to the company's board of directors at its 2014 annual meeting of stockholders.
"Their decision did not involve a disgreement with the company on any matter relating to the company's operations, policies or practices," the filing with the Securities and Exchange Commision said.
The SEC filing comes after Miami Lakes-based BankUnited completed a public offering of 11.8 million shares at $33.145 per share. The proceeds were used to pay off several private equity groups that had invested in the bank, linked to the directors who are not seeking reelection. The groups include Blackstone Capital Partners, Carlyle Partners and Centerbridge Capital Partners.
Investor Ross, alone, sold 3,131,307 million shares at $33.145 a share, for more than $103 million, a separate SEC filing shows.
In January 2011, BankUnited completed its initial public offering, raising nearly $800 million, at 29 million shares priced at $27 a share.


text:
Jonathan Allen | April 27, 2012 | Comments
Aims to bring analytical power of paid search to video content.
What does the new Google privacy policy, YouTube and Trueview metrics all have in common? They form the foundation of Google's latest AdWords platform change which brings video advertising out of the realm of huge budgets and TV ad campaigns and into the hands of small to medium sized businesses all over the world.
Google aims to bring the analytical power of paid search advertising to video content and use the same type of bidding model to make video advertising available, affordable and measurable for everybody. The most significant change for experienced video advertisers is that campaigns will now be able to demographically target 10x more users on YouTube.
Just like paid search, where you only pay per-click to your website regardless of how many times your ad is shown, with AdWords for video you only pay per-view of your video ad. What is more, with TrueView pricing you only pay every time a user watches your entire video ad. Should a user skip your video ad (by pressing the skip button) in the first 30 seconds or before the end of your video ad, you will not be charged. If your ad runs over 30 seconds, you will be charged a cost-per-view at the 30 second mark.
There are four types of TrueView ad formats, which enable you to determine exactly where you want your ad to appear on YouTube and the Google Display Network (GDN). These ad formats are:
These essentially control whether you want your video ad to appear as a pre-roll to other videos, in search results, at the end of other videos or in the related videos section.
It's really simple to get started. All you will need in advance is a YouTube account. All you have to do is go to AdWords.Google.com/Video and link up your YouTube account. To celebrate the official launch of this product Google is also giving away a total of $50 million in free advertising credits worth $75 per account.
Now that AdWords for Video is out of beta, Google has released a slew of new features for users to target and analyze the impact of your video ads. Here is a quick overview video of the AdWords for Video features and an explanation on how to get started (the key points in the video describing new features are linked below).
TrueView Format Selector - Now with the four TrueView ad formats you can opt out of specific types. For example, disabling in-search ads will be denoted as a partial opt in to the Google Display Network.
Demographic Targeting - Google's new all-in-one privacy policy for all Google accounts means that advertisers can now target video ads by age and gender across all Google properties. This actually represents a fairly significant change according to SEW Video expert Greg Jarboe.
"Last year, about 2 to 3 percent of the people who visited YouTube had YouTube accounts which meant that they were the only ones that YouTube could target by demographics," Jarboe said. "Now, with the linking of Google and YouTube accounts, 5 to 10 times more people can be targeted by demographics. What was just an interesting capability has now become a significant opportunity."
Interest/Topic Targeting - Not a new feature per se, but with Google's all-in-one privacy policy now applicable to YouTube, interest/topic targeting is likely to also have a broader reach then it previously did. What used to be hosted separately as the YouTube Video Targeting Tool has now been decommissioned and re-emerges in the AdWords for Video interface.
Downstream Video Analytics - the AdWords for Video reporting interface also has a cool new feature which shows you what videos users watched on your YouTube channel after arriving via a video ad. AdWords for Video product manager Lane Shackleton told SEW that the aim of this metric is to show advertisers that "paying for video views can drive follow on viewership on their YouTube channel". The downstream analytics tool will show many further free views to your channel video advertising can generate.
Advanced Bidding - Unfortunately we don't have too much information on this feature but it looks like advertisers will be able to optimize their bids against "engagement" metrics such as video shares and +1 button clicks.
In tandem with the launch of AdWords for Video, YouTube will be launching a playbook for advertisers which follows the techniques and strategies of some of their most successful video advertisers.
Shackleton shared some tactics that seemed to be working for some of their advertisers.
Some products, such as the make and model of a camera, are easy to describe in text. But others need sight, sound and motion to fully communicate their value. These products are ideally positioned to be advertised on YouTube and can generate significant direct sales.
The TrueView video metric means that video ad plays count as true play counts on your video and YouTube channel. Use your video ads to highlight the interesting content you have on your channel in general. Make the ad a teaser for your YouTube channel and aim to generate more downstream clicks on your channel.
Think about placement and optimize the length of your video to work for the best placement. For example, In-Search ads might suit longer and more informational content whereas in-stream ads might need to be much shorter (bite-size) and instantaneously engaging.
The winners in the competitive bidding landscape of AdWords for Video will be those advertisers who engage most effectively with placement, ad timing and demographics. Focus on view rate (like CTR in search) to get the best price for your video views.
This article was originally published on Search Engine Watch.
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Jonathan Allen is the President of Longneck & Thunderfoot, an agile content company based in New York City and part of the Columbia Startup Lab.
Formerly, the Director of Search Engine Watch, he joined Incisive Media's editorial team in December 2009, and was responsible for setting the editorial agenda, sourcing talent and delivering a brand new site design in May 2011. In May 2012 Search Engine Watch won the Gold Azbee National Award for "Online Excellence, New or Relaunched Web Site" from the American Society of Business Press Editors.
Jonathan has spoken at the largest search conferences – SES New York, SES San Francisco, SES London, SES Chicago – as well as PubCon, KoreaComm and SIPA. His presentations are focused on topics such as the future of SEO, video optimization, and marketing automation. He has also provided industry commentary on breaking news for the BBC, the Boston Globe and Deutsche Welle.
Before Incisive, Jonathan worked as an SEO consultant and also co-founded Moblog:tech, an award-winning mobile social networking software provider and owner of the mobile social network Moblog. In 2009, Moblog won the Experimental & Innovation Webby award for a collaborative mapping project with Shozu and in 2008, Moblog:tech's build of Channel 4's Big Art Mob won the community engagement award at MediaGuardian Awards for Innovation (MEGAS), The Royal Television Society's On The Move Award, and 3 BAFTA nominations.
October 16, 2014
November 3-6, 2014
November 11-12, 2014
Dec 2-4, 2014
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September 17, 2014
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text:
Expired Property Listing
This property is disabled.
Mountain Moments to Remember in Idyllwild
- bedrooms 4
- baths 3
- sleeps 12
- pool no
- hot tub yes
- pets allowed yes
- type House
- community
rates (USD)
/ night
$ 1,500 - $ 2,830 / week
$ 4,200 - $ 6,300 / month
vacation rental description
view previous photoview next photo
Location: Idyllwild, Inland Empire, California, USA (2 Hrs. from San Diego or L.A., 45 minutes from Palm Springs)
Accommodations: Lodge, 4 Bedrooms, 3 Baths (Sleeps 12)
Lookout Lodge is often referred to as "the most beautiful home in Idyllwild". It sits on an acre of land adjacent to the national forest enjoying 360 degree views of the San Jacinto Mountains, Garner Valley, oak, pine and manzanita forests, a waterfall and stream, and when conditions are just right, the ocean. Both sunrise and sunset are viewed from this unique property. Garnishing over 3500 square feet of living space, spectacular views, 4 bedrooms 3 bathrooms, sleeping twelve, makes the Lookout Lodge an ideal choice for family gatherings as well as small groups * Should your group exceed twelve, there is a neighboring cabin which sleeps 6 comfortably and can be rented simultaneously. This home is owned by Jim and Kathleen Keifer whose works are featured in art galleries around the world. There is a small studio gallery on the property and the home itself is filled with original art, all for sale. A private tour is available upon request. Lookout Lodge is perfect for that intimate mountain wedding accommodating up to fifty guests. The garden area features many recreational choices including a trampoline, ping pong table, croquet and bocce gear for lawn sports, hot tub and a large cooking grill. There is an extensive library with hundreds of films, CD's and games in the downstairs media room, all for your enjoyment.
house rental amenities
- Clothes Dryer
- Linens Provided
- Parking For RV/Boat/Trailer
- Washing Machine
- Woodstove
- Wood Fireplace
- Beds
- Queen
- King
- Entertainment
- Ping Pong Table
- Hot Tub
- Video Library
- Satellite Or Cable
- Videogames
- Kitchen
- Microwave
- Cooking Utensils
- Dish Washer
- Full Kitchen
- Ice Maker
- Refrigerator
- Outdoor Features
- Gas/Electric BBQ Grill
- Deck/Patio
- Balcony
- View/Location
- Mountain View
- Communications
- Wireless Broadband
activities and attractions in Idyllwild San Bernardino Area
- Fitness Center
- Rock Climbing
- Boating
- Wildlife Viewing
- Cycling
- Hunting
- Hiking
- Fishing
- Mountain Biking
- Restaurants
- Cinemas
- Health/Beauty Spa
- Live Theater
- Horseback Riding
- Sight Seeing
- Antiquing
- Shopping
- Sledding
vacation rental rates
Rates vary as to number of guests, season or holiday schedules.


text:
Average Annual Expenditures of all Consumer Units by Region and Size of Unit: 2000 to 2005
The Statistical Abstract files are distributed by the US Census Department as Microsoft Excel files. These files have data mixed with notes and references, multiple tables per sheet, and, worst of all, the table headers are not easily matched to their rows and columns.
A few files had extraneous characters in the title. These were corrected to be consistent. A few files have a sheet of crufty gibberish in the first slot. The sheet order was shuffled but no data were changed.
The tables that were changed (this is table 665):
0166 0257 0362 0429 0445 0446 0459 0461 0462 0464 0465 0466 0467 0469 0479 0480 0481 0482 0483 0484 0485 0486 0487 0559 0628 0629 1144 1227 1231
This dataset consists of a table of 195 rows and 20 columns.
In dollars. Based on Consumer Expenditure Survey. Data are averages for the noninstitutional population. Expenditures reported here are out-of-pocket. A consumer unit comprises either: (1) all members of a particular household who are related by blood, marriage, adoption, or other legal arrangements; (2) a person living alone or sharing a household with others or living as a roomer in a private home or lodging house or in permanent living quarters in a hotel or motel, but who is financially independent; or (3) two or more persons living together who pool their income to make joint expenditure decisions. Financial independence is determined by the three major expense categories: Housing, food, and other living expenses. To be considered financially independent, at least two of the three major expense categories have to be provided by the respondent
- Components of income and taxes are derived from "complete income reporters" only.
- Data are likely to have large sampling errors
- Prior to 2000, gifts of alcoholic, personal care products and services, and reading maerials were included in "All other gifts."
Public Domain (Government Work)
This dataset was prepared by the government and is therefore in the public domain. There are no restrictions upon its use.


text:
Discover Adionomy, Your Online Radio Advertising Campaign, Fast, Easy and Custom-Made!
Digital Emotion is one of the many productions of Cat Music, a Dutch production team consisting of the members of a former 70s rock band, called Catapult. After prior success with Catapult, the team changed strategy and started producing. Acts are Rubberen Robbie, Tower, The Monotones, Master Genius, X-Ray Connection and... Digital Emotion. All hits of Digital Emotion are written and produced by Adams & Fleisner.


text:
Nollen Home Theater
(11) Pendelton Collection door and molding style
About this custom design...
The Nollens designed this four piece
home theater with remote controlled plasma lift. The
lift raises the plasma television in front of the bookcase
hutch. Hand fit moldings make this grouping appear as
one piece.
Selected Cabinets
Click on image for details
The Custom Shoppe | 300 Air Park Drive |
Watertown, WI 53094 | 1.920.262.9700
Copyright 2009, The Custom Shoppe. All rights


text:
CUI News
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2014 CUI Brownie Awards – Nominations Now Open!
CUI News, Wednesday, July 30, 2014
CUI is now accepting nominations for the 2014 CUI Brownie Awards. The theme for this year's event is Regenerate: Recognizing the impact of brownfield redevelopment in city building. The 2014 conference and awards will be held on Wednesday October 29th at the Daniels Spectrum in Toronto. CUI will accept nominations in 8 categories: 1. REPROGRAM: Legislation, Policy and Program Initiatives2. REMEDIATE: Sustainable Remediation and...Read more
LightSavers Canada and CUI featured in Corporate Knights Article
CUI News, Friday, July 18, 2014
LightSavers Canada, a program of the Canadian Urban Institute, is featured in an article on CORPORATE KNIGHTS website today: BC BACKS LED STREET LIGHTS FOR MUNICIPALITIES http://www.corporateknights.com/article/bc-backs-led-street-lighting-municipalities #brightlights #healthycitiesRead more
Smart Lights, Healthy Cities - LightSavers Canada provides technical support and research on ground-breaking LED procurement program for BC Government
CUI News, Thursday, July 17, 2014
Smart Lights, Healthy Cities BC Government Announces New Procurement Program to Support Major LED Streetlight Conversion Initiative LightSavers Canada provided technical support and research on this ground-breaking procurement program that will support the eventual conversion of up to 330,000 streetlights across the Province of British Columbia. Coordinated by Shared Services BC, this initiative enables public entities to acquire pre-qualified LED...Read more
Celebrating 11 Years of Urban Leadership in Canada
CUI News, Tuesday, June 24, 2014
FOR IMMEDIATE RELEASE Conversations to Inspire the Urban Agenda 11th Annual Urban Leadership Awards happening Tomorrow! Program to recognize individuals making a profound impact on the quality of life in Canada's urban regions. TORONTO, June 24, 2014 / CUI / Tomorrow the Canadian Urban Institute (CUI) will bring together city builders and place makers from across Ontario to celebrate individuals making a profound...Read more
CUI Proud Supporter of 2014 Canadian Water Summit
CUI News, Tuesday, June 17, 2014
TORONTO (June 18, 2014) CUI Proud Supporter of 2014 Canadian Water Summit CUI is proud to support the 2014 Canadian Water Summit happening Wednesday, June 18, 2014 at the International Centre in Toronto. The Summit is Canada's largest multi-stakeholder water conference with the key purpose of putting the spotlight on urban water issues. Program Highlights Cities and Water • Christopher Gasson, Publisher, Global...Read more
CUI Partners with International Academy for Design & Health to Present Pre Congress Symposium
CUI News, Friday, June 13, 2014
TORONTO, June 13, 2014 / The 10th Design and Health World Congress & Exhibition will be held in Toronto July 9-13. Organized by the International Academy for Design & Health, in partnership with the Ontario Ministry of Research & Innovation, OCAD University and the University of Toronto, John H. Daniels Faculty of Architecture, Landscape, and Design, this 5-day event will...Read more
CUI to Present 11th Annual Urban Leadership Awards: Inspiring the Urban Agenda
CUI News, Monday, June 02, 2014
For Immediate Release Program to recognize individuals making a profound impact on the quality of life in Canada's urban regions. TORONTO, June 2, 2014 / CUI / On June 25, 2014 the Canadian Urban Institute (CUI) will bring together city builders and place makers from across Ontario to celebrate individuals making a profound impact on the quality of life in Canada's urban...Read more
We're Hiring! Program Manager, LED Lighting Technologies, LightSavers Canada
CUI News, Sunday, June 01, 2014
Do you want to help us deliver solutions to create vibrant and resilient communities? If so, the Canadian Urban Institute has the opportunity for you! CUI is seeking a highly organized, entrepreneurial and motivated program manager.The role responsibilities include: Assist in creating a Stakeholder Consortium for Parking Lighting • Support the Director in developing a national consortium to develop momentum towards modernizing lighting in...Read more
CUI featured in May/June Edition of ReNew Canada: Room to Grow
CUI News, Monday, May 05, 2014
Room To Grow: Planning for communities to accommodate future population growth requires smart densification and a clear vision. View "One of the hardest things is the disconnect between where policymakers think we should go and where the market is willing to go. The potential to go wrong is when policymakers try to force density where it isn't welcome. You see that...Read more
CUI to Present at 2014 Land & Development Conference May 13
CUI News, Thursday, May 01, 2014
TORONTO (May 13, 2014) CUI President Peter Halsall will present at the 10th Annual Informa Canada Land & Development Conference on May 13, 2014. This conference focuses on: financing, construction, development, investment and leasing market activity impacting the region. The panel session titled The Boom in Office Development will be moderated by Sandy McNair, President, Altus InSite and will review the...Read more
Career Opportunity: Urban Systems Manager
CUI News, Monday, April 14, 2014
Do you want to develop solutions to create vibrant and resilient communities? If so, Canadian Urban Institute has the opportunity for you! CUI is seeking a highly organized and motivated engineer and/or project manager. The role responsibilities include: providing technical direction in projects dealing with municipal infrastructure, asset management and economic development, organizing project resources to ensure deliverables meet scope, schedule, quality requirements, maintaining effective...Read more
CUI Announces the 2014 Urban Leadership Award Recipients
CUI News, Sunday, April 13, 2014
CUI will present the 11th Annual Urban Leadership Awards: Inspiring the Urban Agenda on June 25, 2014 at the Berkeley Church. This year we introduce a new format, where award recipients participate in a panel moderated by CORPORATE KNIGHTS President Toby Heaps, to inspire the urban agenda for the year ahead. These awards celebrate individuals making a profound impact on the...Read more
CUI and LightSavers Canada featured in Spring 2014 edition of Corporate Knights: Street Smarts
CUI News, Thursday, April 03, 2014
TORONTO Corporate Knights Spring 2014 edition, released earlier this week features an article about LightSavers Canada, an initiative of Canadian Urban Institute. Street Smarts, written by Toby Heaps, looks at how municipalities are making the switch to LED streetlights but that a large number still remain "in the dark" on how to pay for it. Excerpt: "The product is now mature, it...Read more
Special Invitation: Sustainable Sweden Study Tour & World Bioenergy Conference June 1-6, 2014
CUI News, Tuesday, April 01, 2014
June 1-6, 2014, Business Sweden – The Swedish Trade & Invest Council, in cooperation with the Swedish Energy Agency, invites you to participate in a Study Tour to Sweden, including a visit to the 2014 World Bioenergy Conference. The deadline to apply is Friday, April 25th. About Business SwedenBusiness Sweden is an organization built on proud traditions from the Swedish Trade Council...Read more
CUI Plays Role in Recognizing Outstanding Leadership in the Canadian Public Sector
CUI News, Thursday, February 13, 2014
TORONTO On February 13, 2014 CUI's Andrew Farncombe participated as a municipal sector juror for the 2013 IPAC/Deloitte Public Sector Leadership Awards The awards program recognizes public sector organizations – including city governments and municipalities – that have demonstrated a bold leadership role to improve Canada through advancements in public policy and management. "This was a great opportunity for CUI to bring...Read more
CUI to Present at 2014 Creative City Summit (June 11-13, 2014): Bringing the Humboldt Story to Hamilton
CUI News, Tuesday, February 04, 2014
HAMILTON (June 11-13, 2014) LOVE YOUR CITY: Transforming Communities through Culture is the theme for 2014 Creative Cities Summit. Jeff Evenson (Canadian Urban Institute) will present at the Summit on CUI's work in Humboldt, Saskatchewan: "Our Humboldt: A Culture-led Growth Management Strategy". About Creative City Summit The 2014 Summit theme focuses on communities creating conditions in which culture can thrive. Presenters and...Read more
CUI featured in Canadian Architect
CUI News, Tuesday, February 04, 2014
Canadian Architect is helping CUI spread the word about our brand new event series: +PLACE MAKERS. An excerpt from the article: The Canadian Urban Institute (CUI) kicks off a brand-new event series: +PLACE MAKERS with a presentation of global research findings. Dr. Roger Keil of the City Institute at York University, supported by members of the Greater Toronto Suburban Working...Read more
2013 Brownie Award Winner: Halifax Power Station Featured in The Globe and Mail
CUI News, Tuesday, January 28, 2014
HALIFAX (Jan 28, 2014) The Canadian Urban Institute is featured in today's edition of The Globe and Mail. The article is about the Halifax Power Station, recipient of a 2013 Brownie Award, recognizing excellence in building-scale redevelopment of an industrial site. "They've repurposed [the building] in a creative way," says Peter Halsall, the institute's president, who praises the project's adaptive reuse, its...Read more
CUI President Peter Halsall and Senior Associate Todd Latham Officially Announced as part of GLOBE 2014
CUI News, Thursday, January 23, 2014
For Immediate Release VANCOUVER - On Thursday, March 27,2014, CUI President Peter Halsall and Senior Associate Todd Latham (Founder, ReNew Canada), alongside John Williams (CEO, Impact Infrastructure LLC, USA) and Timothy Psomas (Chair, Institute for Sustainable Infrastructure, USA) will participate in the following GLOBE 2014 session: The Infrastructure Deficit – A Problem or an Opportunity? Exploring cutting edge financing models and investment...Read more
Building Resilient Cities: CUI Partners with GLOBE 2014
CUI News, Tuesday, December 17, 2013
The Canadian Urban Institute has partnered with GLOBE 2014. This 3-day series takes place in Vancouver from March 26-28, 2014 and attracts top executives, municipal officials and thought leaders from more than 50 countries. Peter Halsall, President, Canadian Urban Institute will participate in the Infrastructure Deficit: A Problem or an Opportunity session. "CUI's work provides evidence to support decisions that lead to...Read more
Making the Move: CUI calls Transit Panel work ground-breaking and fundamental to good decision-making
CUI News, Thursday, December 12, 2013
For immediate release: The Canadian Urban Institute (CUI) congratulates the Transit Investment Strategy Advisory Panel, led by Anne Golden, on the release of the fourth and final report today: Making the Move: Choices and Consequences. Peter Halsall, President and CEO of CUI responded to the release of the report by issuing the following statement: "The work done by the Panel to demystify the...Read more
A Suburban Office Park Designed with an Urban Vibe
CUI News, Tuesday, November 26, 2013
The Canadian Urban Institute (CUI) was featured in The Globe and Mail. The article titled "A Suburban Office Park Designed with an Urban Vibe," appeared in the Tuesday, November 26th edition and references two important CUI reports. Greater Toronto Area: A Region in Transition which can be read here and The New Geography of Office Location found here. Below, an...Read more
Supporting Post-Disaster Economic Recovery in the Philippines
CUI News, Thursday, November 14, 2013
Supporting Post-Disaster Economic Recovery in the Philippines Over the past 15 years, the Canadian Urban Institute has been working with the local governments in Iloilo city region that has been hit hard by hurricane Yolanda. As a result of our relationships and knowledge, we are providing valuable information to the Government of Canada regarding the conditions on the ground. This information...Read more
Helping Humboldt: Delivering a Culture-Led Growth Plan in Saskatchewan
CUI News, Wednesday, October 30, 2013
The Canadian Urban Institute (CUI) is working with the City of Humboldt to develop a culture-led growth strategy. The world's largest mining company, BHP Billiton's investment of US$2.6 billion in the Jansen potash project located 40 km from Humboldt could result in significant economic growth with as many as 500 new households moving to Humboldt, Saskatchewan. The primary purpose of this...Read more
2013 CUI Brownie Award Winners Announced!
CUI News, Thursday, October 24, 2013
October 24, 2013 – Toronto, ON The 13th annual CUI Brownie Awards were presented at a Gala Dinner at the Westin Harbour Castle yesterday evening . Held in conjunction with the CUI's Canadian Brownfields conference – STILL Making Great Places –the awards celebrate excellence in brownfield redevelopment by transforming contaminated sites into "great places." The CUI recognized winners in seven categories from...Read more


text:
Getting a Restraining Order
Are you being hurt?
If you or a friend are being hurt, contact the Northeastern University Police Department immediately.
- Call (617) 373-3333 or 3333 from a campus phone.
- Call 911 if you are off campus.
Consider ways to leave the present situation safely
The police will assist you in obtaining an emergency order valid until the next court business day.
Once the order has been issued, you will be given a copy and the police will attempt to serve a copy to the abuser.
Remember to keep the order on you at all times.
Obtain a restraining order
NUPD Detectives will assist you at each step of this process and accompany you to all court hearings.
This is a civil complaint.
You will be required to file an application for a Complaint for Protection from Abuse at a local court.
You will then speak to a judge or clerk magistrate to explain what happened and why you need protection.
The judge will then decide whether to grant a Temporary Order which is valid for 10 days.
You will be provided a copy of the order and summons to return to court in 10 days for a hearing.
The abuser will also be sent a summons to appear and the police will attempt to serve the order.
At the hearing, the judge will decide whether to extend the order up to a period of one year based on the testimony heard and evidence gathered.
If the abuse does not appear, the hearing will still be held. You must appear at this hearing or the order will automatically be dismissed.
What if the order is violated?
Violation of an Abuse Prevention "Restraining" Order is a crime.
Contact the Northeastern Police immediately by dialing
(617) 373-3333. If you are off campus, dial 911.
Develop a personalized safety plan
Remember to keep your order with you at all times.
Inform family, friends, neighbors and the local/university police that you have an order.
Identify and have available important phone numbers: police, friends, shelters and hotlines.
Identify a few safe place you can go if you have to leave your home quickly.
Leave extra money, car keys, clothes and copies of important papers with someone you trust.
Increase safety at your home by upgrading physical security, i.e. change locks, increase outdoor lighting, install an alarm system.
Inform your family, roommates, friends, employers, and neighbors that the abuser is no longer welcomed by you and to contact the police if the abuser is seen near your home or place of work.
If you have children, inform your child's school, day care center, etc. of the names of those persons with permission to pick up your child.
If you are thinking of returning to an abusive relationship, first talk with someone you trust about other possible options.
Get involved with a support group on campus or in your community.
You are not alone and you are not at fault.
Know Your Rights! Domestic Violence Facts & Available Resources: http://www.neu.edu/publicsafety/dom_abuse.html
Related link: http://www.casamyrna.org/


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Chief Marketer Network: PROMO DIRECT MULTICHANNEL MERCHANT
All of ChiefMarketer's webinars are FREE and available on-demand for up to one year after the run date.
Aug. 07 2014
Jun. 07 2012
Free Live Webinar: Join us to hear first hand how People's United Bank converged 11 websites into a consistent, confident experience for its customers.More...


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'Don't be complacent over economic recovery,' warn county business chiefs (From The Oxford Times)
When It Happens Panel Get involved: send your photos, videos, news & views by texting 'OXFORD NEWS' to 80360 or email
'Don't be complacent over economic recovery,' warn county business chiefs
9:00am Wednesday 29th January 2014 in News
COUNTY business leaders have warmly welcomed Britain's latest economic growth figures.
They have warned, though, that people should not take the recovery for granted.
Bob Bradley, president of the Oxfordshire Chamber of Commerce, said: "This is entirely consistent with what I am picking up from our members.
"But there is a degree of caution around as there are risks such as the state of the Eurozone and the United States debt. If interest rates start to go up there are definite concerns about how sustainable the recovery may be.
"The message is to capitalise on growth but don't assume nothing will go wrong."
Chancellor George Osborne yesterday claimed the Coalition's economic policies were delivering a "brighter economic future" after official figures showed growth last year was the best since 2007.
Gross domestic product (GDP) increased by 1.9 per cent in 2013 and has now recovered the lion's share of output lost in the recession, according to the Office for National Statistics (ONS).
The pace of recovery slowed slightly in the fourth quarter of last year to 0.7 per cent but economists are predicting an annual improvement of up to three per cent for 2014.
Stuart Roper, chairman of the Oxfordshire Federation of Small Businesses, added: "We are definitely seeing growth and the Chancellor needs to know a lot of it is from small businesses.
"There are definitely more opportunities around and a lot of them are down to us."
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The wait is over! Download the BeerAdvocate app on iTunes or Google Play now.
Get 12 issues / year of BeerAdvocate magazine for only $9.99!
Dark Lager Beer - Kaiserdom Privatbrauerei Bamberg
Displayed for educational use only; do not reuse.
Ratings: 20 | Reviews: 13 | Display Reviews Only:
3.75/5 rDev +15.4%
08-18-2014 15:21:39 | More by dcmchew
3.75/5 rDev +15.4%
07-07-2014 00:15:24 | More by lccrunchy
United Kingdom (England)
3.25/5 rDev 0%
05-31-2014 21:41:37 | More by endless1408
3.48/5 rDev +7.1%
500ml bottle. Footed Pokal glass. Pure black color, very dark brown to the light. Tan foam, close & fairly good retention crown. Aroma profile displays roasted & burnt malts, sweet notes & hints of black cocoa. Dried upfront & slight bitter flavor from roastiness. Light body. Moderate carbonation. Creamy to watery texture. Very low floral bitterness in the middle. Dry bitter finish & roasted aftertaste. Its easy drinkability makes it a flavorful session beer.
04-14-2014 08:24:53 | More by GONZALOYANNA
South Carolina
3.84/5 rDev +18.2%
Picked up at a local grocery store in Shanghai China. Drank from the 500mL can.
S: Has a nice deep malty smell to it with some caramel in there but also hints of smoke. Faint sweetness at the end.
T: Good malty character. Really surprised by this beer. Some milk chocolate in there, and some dark bread. A bit of smoke and roasted malt in the middle, and then a bit more sweetness.
Feel: Pretty smooth and not flat.
Good all around beer for this area. I'm actually really impressed. I will be picking this up again for sure.
04-05-2014 07:34:18 | More by BucBasil
3.36/5 rDev +3.4%
Pours more slowly than a pale beer of the same gravity would. It's not exactly viscous, but it doesn't want to leave the can in a hurry.
You get a generous head, lots of very small bubbles, which hugs the side of the glass.
On the nose there's lots of black malt, almost a roasted coffee smell.
The taste is good, chocolate, nuts, roasted barley of course, a little hop flavour. I soon got used to it.
This could be a real session beer. It doesn't want to be too cold IMO.
01-25-2014 12:26:17 | More by downes51
3.85/5 rDev +18.5%
Poured from a can into a pint glass.
A-Deep black with slight ruby red highlights. It poured a nice creamy tan head that stuck around for a minute. There was some beautiful sticky lacing left on the glass. It looked excellent!
S-burnt malt, roasted malt, and a slight smoky smell.
T-Pretty similar to the nose: smoky malt, roasted barley malt, a slight trace of hops, but overall malt forward. Going in was pleasantly smooth. As it warmed there was a trace sweet malt taste. There was also a light coffee and black chocolate taste, but not too overpowering. It seemed fairly balanced. The aftertaste was dry and a little smoky. Unfortunately, the aftertaste was a little too fizzy and flat.
M-Pretty sessionable. It was lighter bodied-medium bodied and refreshing. It had just enough carbonation to keep me satisfied. The overall body of the beer felt quite full, but in fact it is more moderate.
Overall, a decent beer to drink on the last day of October, although the roasted, smoky, and burnt malt smell/taste did not represent the true schwarzbier style, IMO. Oktoberfest has been fun, now it's time to bust out the full winter gear! I would buy this again. It is good enough to drink more of. Also it is a perfect dark lager to drink while the fall air starts to feel more like winter air. Cheers!
10-31-2013 17:29:58 | More by Jsteez
New York
3.25/5 rDev 0%
10-24-2013 15:36:28 | More by bsm80x
3.29/5 rDev +1.2%
Dark brown color with an abundant off-white head with medium retention and leaving laces.
Aroma of roasted and crude malts, nuts, wheat and grassy hops with notes of dark chocolates.
Similarly, flavor of roasted malts also with dark chocolate and an above average amount of hops for the style, though shadowed by its malt type.
Aftertaste is dry with a medium bitterness.
Medium body and carbonation.
Interesting that some beers grow on you, this one starts at a higher note and then down the slope. Still a fair example of the style.
02-24-2013 17:27:07 | More by FLima
United Kingdom (England)
3.05/5 rDev -6.2%
A 500ml bottle with a BB of Dec 2012. Picked up from Tesco some time back.
Poured into a straight pint glass. A dark brown colour with reddish hints. Forms a large head of creamy beige foam with decent retention; this slowly reduces to a surface layer. Aroma of sweet, dark malt with hints of caramel, nuttiness, mild roasted grain, faint chocolate and earthy yeast. Subtle.
Tastes of dark malt with a mild finish. Notes of caramel, grain, a hint of chocolate and earthy yeast. Slightly sweet; no hop character to speak of. Dry and virtually without bitterness. Mouthfeel is smooth and tingly, with pleasant carbonation but a thin, insubstantial body. Aftertaste of caramel malt and grain.
A pretty average dark lager. Looks OK, but the aroma and flavour are muted and uninspiring. Body could be a bit thicker/creamier. Made for easy, unchallenging drinking - not terrible but lacks depth. No need to seek it out.
12-16-2012 23:07:38 | More by jazzyjeff13
United Kingdom (England)
3.3/5 rDev +1.5%
Purchased at the Tesco supermarket; BB 01/2013, served chilled in a 500ml weizen glass.
A: pours a deeply dark brown colour, coming with abundant fizziness and a 2cm beige foamy head to last.
S: maltiness comes rather understated, leaving room for hints of black dates, brown sugar and sweetened roasted walnuts to sneak through; the semi-rich roastiness from the malts sustain well in the background. Overall the aroma is slightly richer than most other Dunkel I've tasted (not many anyway…).
T: grainy or gristy even upfront, the dark malts present themselves in a bitter-sweet (B>S) fashion, followed by a twist of lager's sourness and then paving the way for the arrival of roasty bitter edges and a hop bitter palate.
M&D: softly carbonated, medium bodied, this Dunkel shows an unusual depth and is in fact quite likeable/drinkable, although this is never my preferred style when it comes to dark lager (I prefer the Czech counterparts).
09-15-2012 19:41:13 | More by wl0307
United Kingdom (England)
2.25/5 rDev -30.8%
09-11-2012 18:52:25 | More by His_Royal_Hoppiness
United Kingdom (Scotland)
3/5 rDev -7.7%
04-17-2012 21:08:24 | More by Hanglow
3.13/5 rDev -3.7%
0.5 liter bottle poured into a Paulaner stein, BB 10/12
a: pours a deep chocolate brown with a three finger tan head. dissipates fairly quickly, nice lacing.
s: roasted malts, some chocolate and toffee, sweet and clean.
t: roasted malts, dark chocolate, toffee, some caramel. a nice creaminess and body, with some hops to balance at the end. a nuttiness at the end as well.
o: a solid munich dunkel. ultimately, perhaps a bit thin mouthfeel, but still enjoyable.
03-30-2012 17:14:43 | More by RodneyFarva
3/5 rDev -7.7%
02-08-2012 14:06:24 | More by nandika
United Kingdom (England)
3.35/5 rDev +3.1%
pours a black body with slight off white head
a good looking beer, best point
charcoal is the biggest hit o n the aroma
nice hint of liquorice on the taste and then fades to a slight sourness
very thin mouthfeel but okay carbonation
I've had worse but I have had far better dark beers.
12-25-2011 21:08:26 | More by EmperorBevis
United Kingdom (Scotland)
3.08/5 rDev -5.2%
Dark brown with three fingers sized bubbly tan head with good retention & some lacing on glass.
Dark smelling with a lot of dark chocolate upfront as well as a slight bit of coffee and perhaps some caramel or toffee blended in their as well. It has quite a strong malty aroma to it and overall it is quite sweet too.
Dark, roasted malt flavour to it that seems to be a lot stronger than the other beers of this style I have tried previously. There is a rich, dark chocolate taste to it that hints at coffee too but this isn't the strongest. I can detect a little barley too and the bitterness in the finish is medium and a has hints of sweetness through it.
Medium bodied and pretty smooth with a fair bit of carbonation and and sort of malty, mildly bitter after taste that lingers for a few seconds.
An okay pint, it has a lot of flavour and a fair bit of depth to it but I felt that it was just that little bit to rich and malty for my taste buds.
Review @ http://abarwithnoname.wordpress.com
11-14-2011 14:56:36 | More by flyingpig
United Kingdom (England)
2.73/5 rDev -16%
Brown 500ml bottle, best before Dec 2011, drank and reviewed mid July 2011.
Deep rustic brown body with a cream coloured head which gave off loads of lacing on the vase shaped glass it sat in.
The smell was of sweet malty molasses, but not overly so, or sickly.
Initially I thought 'what a cracking flavour of maltiness', but sadly the brew soon turned into a boring malty tasting fizzy beverage I didn't like so much.
The body was weak in depth, even for a lager/pilsner based beer: almost watery in fact, even though it was highly carbonated.
Not very impressed.
08-08-2011 16:46:40 | More by BlackHaddock
3.3/5 rDev +1.5%
Dark mahogany colour. Clear. Moderately-large tan head. Fair head retention.
Nutty, bready malt with a touch of melanoidin. Good earthiness.
Slightly tart, dark malt with a little bit of roast. A touch of maltiness but there is a fair amount of dryness. Cardboard.
Mediul body with moderately-high carbonation. Fine.
A fair example of the style with a bit of oxidation letting it down.
06-01-2011 08:43:10 | More by dgilks
3.03/5 rDev -6.8%
Best By date Feb. 3, 2012. 1L can.
Appearance: Dark brown color with a slight reddish cast, almost completely blocking any light. One finger of dense tan colored head with good retention. Left rings of lacing.
Smell: Slightly sweet aroma from malt. Also ripe fruit and yeast.
Taste: Sweet malt, with plenty of roasted flavor. No discernable hops flavors.
Mouthfeel: Meduim-bodied and chewy. Low carbonation. Roasted malt finish.
Overall: Quite drinkable, but one 1L can is enough for me at one sitting.
04-21-2011 12:47:29 | More by dbaker61
Dark Lager Beer from Kaiserdom Privatbrauerei Bamberg
78 out of 100 based on 20 ratings.


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WASHINGTON – The creator of the beloved and critically acclaimed HBO series The Wire offered Eric Holder a counter-offer in response to the U.S. Attorney General's casual plea for another season of the show: I'll do it if you end the war on drugs. "I want to speak directly to…
Read More
By Roy Greenslade, The Guardian
By Katie Halper
By The Conversation
By TBogg
Raw Story is a progressive news site that focuses on stories often ignored in the mainstream media. While giving coverage to the big stories of the day, we also bring our readers' attention to policy, politics, legal and human rights stories that get ignored in an infotainment culture driven solely by pageviews.
Founded in 2004, Raw Story reaches 9 million unique readers per month and serves more than 30 million pageviews.
Copyright © 2004-2013 Raw Story Media, Inc. All rights reserved.


text:
The Browns announced Monday that they have completed new head coach Pat Shurmur's coaching staff, with seven assistants from Eric Mangini's 2010 staff staying on and and five new assistants joining the fold.
The assistants retained are Gary Brown (running backs), Steve Hagen (tight ends), Jerome Henderson (defensive backs), Kent Johnston (strength and conditioning), Rick Lyle (assistant strength and conditioning), Luke Steckel (assistant to the head coach) and George Warhop (offensive line).
Among the new assistants are Ray Rhodes, who was the Texans' senior defensive assistant last season, and Keith Gilbertson, who was the Browns' director of pro personnel in 2010. The other new assistants are Chris Beake (offensive assistant), Chuck Bullough (defensive assistant) and Shawn Mennenga (special-teams assistant).
As expected, Shurmur, who will call plays, will not have an offensive coordinator.
"With our coaching staff now complete, I am excited about the group that we have been able to assemble," Shurmur said in a statement issued by the club. "I believe we have a blend of coaches with a tremendous amount of experience who have proven to be excellent teachers throughout the course of their careers. I have known most of these guys for a considerable amount of time, and not only have I been impressed with their work, but they are all greatly respected around the league. Many of our coaches have been a part of winning programs, which is an important element as well. I feel very good about the strengths that each coach possesses, and I am confident this will benefit our team."
The way we see it
Rhodes and Gilbertson were both assistants under Browns team president Mike Holmgren when Holmgren coached the Seahawks, and both bring considerable experience to a coaching staff led by Shurmur, who is making his debut as an NFL head coach.


text:
|Related Artefacts
Burnt Wire Bush Turkey by Marcia Alice Panangka
Aboriginal men and women in central Australia carve animals from dry wood they find or tree roots they have dug up. They often use the roots of river gum or mulga. They use the shape of the wood to make models of animals. Some sculptures are plain, while others have patterns and symbols relating to their Aboriginal culture.
The Aboriginal artists make the sculptures beside the camp fire. They put a piece of fencing wire into the fire. When it is hot, they burn designs on the wood.
Marcia was born in 1960 at Ltyentye Apurte in central Australia .
Marcia moved to Titjikala (formerly known as Maryvale) when she married and has raised two sons. Marcia previously was attached to the Keringke Arts Centre at Ltyentye Apurte, producing silks and artworks. Marcia's work is characterised as fine dotting and circular designs.
Marcia and other artists that make wooden artefacts and sculptures collect the roots from of certain trees found growing around Titjikala. The wood is cut using an axe. Often the animal carvings are decorated by the women using wires heated in the camp fire until red hot. They then burn patterns and designs onto the sculptures.
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The Copyright of all images and documentation remains with Sabine Haider. The Australian Copyright Act protects all artists from unauthorised copying by giving control over original works of art to the artist by law. However depending on the use proposed, Sabine Haider from Central Art – Aboriginal Art Store can facilitate reproduction of works with the permission of the artist as we have developed close relationships over the years with many individual painters and craftspeople.
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The Pointe in Parker
- bedrooms 3
- baths 2
- sleeps 10
- pool no
- hot tub no
- pets allowed no
- type House
- community Lake Moovalya Keys
rates (USD)
/ night
$ 2,100 - $ 2,625 / week
vacation rental description
The house is located in a gated community where the houses are on canals that go out to the Colorado River. It is ideally located on the main canal that connects to the river. It is at a fork in the canals creating a large body of water in front of the house. This makes for easier manuevering of boats. A very large patio and huge wrap around dock make for more enjoyable outdoor living. 5mph speed in the canals make for safe swimming at the house and safe mooring for vessels. The 120' dock is private and belongs to the house. There is 60' on one side and 40' on the other of usable space for mooring.
vacation rental location
property availability
- dates with deals
- unavailable dates
Last modified on 08-19-2014 at 7:42 PM.
email owner view full calendar
Kathy Northcutt 619-445-9595
house rental amenities
The house is fully furnished and stocked. There is a refrigerator, stove, dishwasher, microwave, coffee maker, blender, toaster oven, crock pot, dishes, utensils, serving dishes, seving utensils, gas bbq, large flat screen tv with DVD and cable, CD stereo, linens, and bathing towels. Wireless internet service is also available. There is air conditioning in the summer and a fireplace or heater in the winter. There are 2 queen size beds in 2 of the bedrooms and a king size bed in the master. The patio has patio furniture and a large palapa and trees for shade. The dock has a ladder off the front and a ramp at the back making access to the water possible for any age. This house is located right across the canal from my other house #72478 (Park Place) making it nice for larger groups to all be together.
activities and attractions in Parker Arizona
Since the house is connected to the Colorado river, water sports are the order of the day. The Parker Strip is wide and deep with few sand bars to worry about. Since the house has easy access to the river you can go out for the day or just for an hour. There are also bars located along the shoreline. There is also the Blue Water casino at one end of the Parker strip or Buckskin state park at the other end. When the weather is cooler there is a very nice golf course near by. There are also a lot of off road trails to explore in the winter. There is also Lake Havasu about an hour away with the famous London Bridge.
vacation rental rates
The regular rate is $400 a night with a 3 night minimum plus cleaning fee.
The holiday rate is $475 a night with a 4 night minimum plus cleaning fee. Holidays include Memorial day, July 4th, Labor day, Tube Float, and Feb and Oct off road races
Weekly discount-$50 a night off the regular rate (for 7 nights using one weekend).
A monthly rate of $1700 plus cleaning fee is only available in the winter with a 2 person occupancy
Kathy Northcutt 619-445-9595
accepted payment methods
Prior to paying a reservation deposit, always verify payment instructions by calling the phone number displayed on this listing page. By contacting the owner directly and paying by an approved method - credit card, paypal, check or bank transfer - your reservation payment will be protected up to $1,000.Learn more in our Security Center
Top things to do in Parker
- Watersports
- Fishing
- Gambling
- Hanging out at the river bars
Top things to do on a budget
- Floating in the canals
- Tanning on the patio
- Having cocktails on the patio
- Swimming
Top things to do with a family
- Visiting the London Bridge
- Picnicing at Buckskin state park
Kathy Northcutt , owner


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The Good Life Kitchen Cooking Class Series - Aug. 23rd
Learn about recipes for your farmer's market finds starting at 11 a.m.
Huntsman Cancer Institute (HCI) is part of the University of Utah Health Care system. We serve cancer patients throughout the Intermountain West and provide academic and clinical training for future physicians and researchers.
Hope through Research: Our scientists focus on understanding cancer from its beginnings to create safer, more effective treatments. Researchers also study how genes function to look for ways to prevent or even reverse genetic changes that cause cancer.
Hope through Education: We provide free, expert and accurate information about cancers, risk factors, screening, prevention, and treatments to patients, family members, caregivers, physicians, and the public.
Hope through Patient Care: Our teams of doctors and health care professionals work together to provide compassionate, state-of-the-art care.
Blueprint for next generation of chronic myeloid leukemia treatment
8,000-year-old mutation key to human life at high altitudes: Study identifies genetic basis for Tibetan adaptation
Be Well Utah: Preventing cancer with Huntsman Cancer Institute
Skin Cancer and Moles
Utah dad inspires young sons award-winning video


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The Ryan Dempster saga enters its fourth day (he was nearly traded to the Atlanta Braves on Monday) with Dempster still officially a Chicago Cub.
That could change soon, though, says CSN Chicago's David Kaplan:
Team Theo and Co. are looking at a number of scenarios, with sources telling me this afternoon that a multi-team trade that would send Ryan Dempster to his preferred destination, the Los Angeles Dodgers while netting the Cubs a handful of prospects built around starting pitching is getting closer to happening.
This is all happening because Dempster exercised his 10-and-5 rights and vetoed a deal that would have sent him to the Braves, after which:
Epstein reportedly was livid when the deal with the Braves fell through and it appears for good reason as a handful of scouts that I spoke with in other organizations felt that the Cubs had made a tremendous deal getting Randall Delgado for essentially a two-to-three month rental.
There are now fewer than five days until the non-waiver trading deadline, which hits at 4 p.m. ET on Tuesday. Stay tuned.
For more on the Cubs, please visit Bleed Cubbie Blue and SB Nation Chicago.


text:
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Stone Of Destiny - The Olde Burnside Brewing Company
Displayed for educational use only; do not reuse.
Ratings: 36 | Reviews: 10 | Display Reviews Only:
5/5 rDev +21.4%
If this beer had a XXX, I'd XXXX it. It's really that delicious. They only put it out on special occasions. Haven't seen it in years. I just remember it being the bomb-diggity. If you see it in the packy, I suggest you snag some.
Packy (package store) is CT lingo for a liquor store. Funny, when we go out of state and ask directions for a package store we're always directed to the local post office.
05-31-2014 04:55:12 | More by Beeraphernalia
4/5 rDev -2.9%
12-16-2013 02:12:51 | More by tobelerone
4/5 rDev -2.9%
10-03-2013 16:07:28 | More by Kmlund
3/5 rDev -27.2%
08-26-2013 20:55:35 | More by dfhhead25
3.75/5 rDev -9%
07-16-2013 01:53:05 | More by DonFrap
5/5 rDev +21.4%
06-04-2013 17:38:36 | More by hefferee
New York
3.75/5 rDev -9%
06-03-2013 18:03:19 | More by ElijahRivera
4/5 rDev -2.9%
06-02-2013 22:53:02 | More by Janeinma
4/5 rDev -2.9%
06-01-2013 15:36:44 | More by Davepoolesque
3.75/5 rDev -9%
03-17-2013 06:08:21 | More by BMart
4.25/5 rDev +3.2%
02-03-2013 01:00:12 | More by DrunkinYogi
4.25/5 rDev +3.2%
02-02-2013 00:52:52 | More by FutureOBrew
3.44/5 rDev -16.5%
Got this beer from a colleague. It poured out a deep dark brown with a little less head retention than I would have expected. On the other hand, the legs stuck to the sides of the glass more than I thought from the original pour
The first whiff of this beer hits you with vanilla, sweetness, oak and booze. Once in the mouth the aroma is backed up with a smooth and sweet flavor. The barrel aging seems to overpower the beer quite a bit, but with all the components it's hard to believe this beer is 12%. The beer is a little thinner than expected, but with that much alcohol in it, it may just be that this cuts the body.
Overall this was a unique beer and certainly the rum barrel adds vanilla and sweetness that sets it apart from boubon barrel aged beers. The barrel overpowers the beer a bit and it is perhaps a bit boozy, but at 12% what do you expect?
01-25-2013 02:27:18 | More by Smoke
4.39/5 rDev +6.6%
A- Pours a deep brown body with reddish and mahogany hues. Thin 1-finger khaki head sits fow a few seconds, but when it falls, it falls to a nice solid cap. A touch of sticky lace left behind.
S- A liqour aroma at first, bourbon, oak, vanilla, and a little milk chocolate. All seems in balance. Very nice, albeit a little muted.
T- Wow. This is damn good. Vanilla, bourbon, a slight brandy-ness to it as well. Nice milk chocolate flavor, too. Just like the aroma, the taste is wonderfully balanced.
MF- Moderate body, but is it every a silky texture. Carbonation level is perfect here.
O- A very fine beer. The bourbon flavor and aroma is there, but in no way overpowering. You'd never know that this is a 12% beer. Another fine job by the guys in Hartford!
01-16-2013 21:31:07 | More by LtCracker20
4.25/5 rDev +3.2%
01-09-2013 05:21:25 | More by billybrew21
4.25/5 rDev +3.2%
12-14-2012 02:24:07 | More by Gregwa
5/5 rDev +21.4%
06-06-2012 20:07:01 | More by Brunobstyle
New York
4.25/5 rDev +3.2%
06-04-2012 14:19:56 | More by GrindFatherBob
4.5/5 rDev +9.2%
Black & Tan? Really? I don;t see that from my meager experiences with Guinness and Bass. This stuff is far supperior I'm happy to report, a whole nother category. A solid dark brown color with a fair light tan head with streaky modest lacing. The aroma brings out big time caramel chocolate, vanilla toffee and sweet prune over nutty peat. Wow - Home Run for the nostrils. The flavor is another heavy hitter bringing out deep dark fruits with a maple-vanilla sweetness and a caramel touch. I get some root beer and fermented licorice. This is a chewy beer in flavor but the body is more of a medium light deal with a bit of a syrupy touch that is nicely cut by an active carbonation. Just a delightful dark brew full of sweet and earthy goodies that will put a smile on your face. Awesome brew!
05-19-2012 03:18:04 | More by Durge
3.75/5 rDev -9%
05-01-2012 20:10:26 | More by sailorastro
2/5 rDev -51.5%
04-29-2012 16:41:35 | More by Mebuzzard
4.25/5 rDev +3.2%
04-05-2012 01:13:30 | More by raythealeman
3.5/5 rDev -15%
02-18-2012 09:02:46 | More by chrispoint
4.1/5 rDev -0.5%
Poured into a snifter at room temperature.
The beer pours a dark cocoa brown with creamy beige head of 1 cm that remains as a surface layer throughout the sampling. Aroma is rich with bourbon and vanilla, brown sugar and some dry oak notes.
First sip reveals an average to thick body with mild rolling carbonation. Smooth silky texture.
Flavor is as the nose advertised with bourbon and brown sugar mixing with a bit of dark chocolate and roasted malt. Where is the booze? 12% is very well hidden. I also dont really get any hop characteristics.
This is a very drinkable 12% brew that features malt through and through with good complexity.
THe cobalt blue bottle is a cool presentation but the cost is a little high, still worth it in my opinion, I just wouldnt make this my nightly beverage.
01-24-2012 04:56:02 | More by sulldaddy
3.73/5 rDev -9.5%
swing top bottle poured into a Chimay chalice
A- a hazy brownish black, the color resembles a trappist quad almost. Fizzy off white head disperses quickly, with slight lacing.
S- The aroma is a mix of a big vanilla fragrance, chocolate, coffee and some hints of alcohol.
T- Im picking up vanilla chocolate and small subtle hints of oak. Its a descent taste not bad but not really what Im looking for. To me its a little like drinking a cream soda. Just to sweet for me.
M- Light bodied beer with a Chimay like fizzy carbonation.
O- I went into this beer expecting something a bit bigger bodied. Like i said before a little sweet tasting for me and the price tag is just to much. Its not a bad beer but for $12.99 it wont be in my fridge again. This is a one and done for me.
01-19-2012 23:43:13 | More by HopsHopenstein
Stone Of Destiny from The Olde Burnside Brewing Company
90 out of 100 based on 36 ratings.


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The Hoppe's No.9 Challenge!
So you think your barrel is clean? Well here's a way to check how good your cleaning method really is.
Clean your barrel as you normally would but before putting it back in your safe run a patch soaked in Hoppe's No.9 through it. Leave it to soak overnight or at least 12 hours and then run a clean dry patch through and see what colour it comes out.
Usually it'll be either a green or blue colour which indicates that there's still some copper fouling present and the barrel could use some extra cleaning.
The picture below is of a patch that came from a supposably clean barrel. The barrel had been given a Sweets 7.62 treatment the night before but it had failed to remove all the copper.
I leave Hoppe's in my barrels permanently and it'll usually take several overnight applications before no more colouration occurs. Probably not really necessary but it's nice knowing the barrels are as clean as possible.
Category: Articles, General, Jason Lownds
Comments (1)
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How corrosive are cleaners such as this?
I've read some articles that specifically state NOT to leave cleaners in the barrel for more than the recommended time.