text:
£1,395 Monthly
Greenhaze Lane, Cambourne, CB23 5EF
A 4 bedroom property in popular new town of Cambourne. Close to local amenities including primary schools, nursery, supermarket, chemist, pub and restaurants. Within easy access of commuter routes to London, Cambridge, Huntingdon, Bedford and Papwort
£1,150 Monthly Let Agreed
Collingwood Drive, Longstanton, CB24 3GW
LARGE Detached 4 bedroom house in popular VILLAGE location of Longstanton. Within easy access of commuter routes to Cambridge, Huntingdon and London. Many local amenities including SHOP, POST OFFICE, PUB, CHINESE TAKEAWAY, primary schoolcatchment are
£795 Monthly Let Agreed
Mayfield Way, Great Cambourne
2 bedroom coach house in the popular town of Cambourne with open plan living area. Within easy access of London, Cambridge, Huntingdon and Papworth. Many local amenities including supermarket, chemist, dentist and doctor surgeries, primary schools, p
£750 Monthly Let Agreed
Furrowfields, St. Neots, PE19 6GU
A nearly new 2 bedroomed house on the ever popular Loves Farm development. Within easy access of commuter routes to London, Bedford, Cambridge, Huntingdon and St Neots. Local amenities include supermarket, leisure centre and schools. More details ›
£725 Monthly
North Lodge Drive
Second floor flat in the popular village of Papworth Everard. Close to amenities including shop, Post Office and within easy access to Papworth Hospital, Cambridge, Huntingdon and London.
£650 Monthly Let Agreed
Cheere Way, Papworth Everard, CB23 3NX
Well presented MODERN 2 bedroom flat within easy reach of Huntingdon, Cambridge and LONDON. Also within walking distance of Papworth Hospital and local amenities including post office, shop, fish and chip shop, doctors surgery, primary school, and in
£625 Monthly Let Agreed
Cheere Way, Papworth Everard, CB23 3NX
Well presented MODERN 1st Floor, 2 bedroom flat within easy reach of Huntingdon, Cambridge and LONDON. Also within walking distance of Papworth Hospital and local amenities including post office, shop, fish and chip shop, doctors surgery, primary sch
£4,500 Monthly
Chaucer Close, Cambridge
HIDE AND SEEK - Safe and sound surroundings for your children to enjoy playing thoughout the upcoming summer holidays. This garden is perfect for you and your family to entertain endless BBQ's and those all too precious quiet evenings with
Listed with: haart Cambridge
£4,500 Monthly
Home Farm, High Street, Harston, Cambridge, CB22 7PZ
LocationApproaching Harston from Cambridge the property is set back from the high street and approached by a private driveway.DescriptionA truly stunning, extremely spacious contemporary barn style pro
Listed with: Savills Cambridge
£3,750 Monthly
Tenison Avenue, Cambridge, CB1 2DX
LocationTenison Avenue is located off Tenison Road close to the railway station.DescriptionAn outstanding family home in a prime central Cambridge location within a few minutes walk of the railway stat
Listed with: Savills Cambridge


text:
Dilution vs. Control. It's an age-old debate in the startup ranks. After all, how much money you take certainly impacts the ownership you hold, as well as the control you wield over a startup. It's a touchy subject that often pits entrepreneur vs. investor, and it usually doesn't emerge until things head south, which they… Read More
Success: 'We Make Seattle' video project passes $28,000 Kickstarter goal
Well, it looks like Seattle is going to be getting a cool short film about its tech community. After a successful Kickstarter campaign, the "We Make Seattle" video project is going forward. The idea behind the video is to showcase the very best of Seattle's tech community, promoting the makers, builders and innovators who've helped… Read More
Geek Humor: Now, this is one iPhone 5 you can't miss
Getting excited for the bigger screen on the iPhone 5? Well, just imagine where Apple could take things. Some Seattle geeks did, producing this hilarious parody video, showing an "iPhone 5″ with a screen that's 18 percent thinner and 795 percent taller than previous iPhones. Perfect for those home projects around the house, or reading… Read More
30 sunrises in 30 days: A beautiful look at our region
This is pretty awesome. The one-man graphics company Digital.Meals created a video of 30 sunrises in Seattle and Tacoma over 30 days, picking iconic locations such as Gas Works and Kerry parks, as well as more ordinary streetscapes. I've watched the video a few times now, and I'm reminded each time of what a beautiful… Read More
Ask Andru: How can I make my iPad louder?
In today's Ask Andru column, we get a question about an issue with the iPad that we've actually experienced ourselves for years. Ever since the release of the original iPad, it's been painfully obvious that the speaker on the Apple tablet isn't exactly up to snuff. It's fine if you're somewhere quiet, but go somewhere that… Read More
Seattle animation startup launches with first project, a Startup Foundation animation
A new startup specializing in explanatory animated videos is launching in Seattle this week — and for a group of people taking the entrepreneurial plunge, it would be hard to imagine a more appropriate first project than this video. The startup, called What Now? Exactly! made this animation for the formal debut this week of… Read More
Nat Brown's advice to wannabee entrepreneurs: Unlearn the nasty habits of big companies (Video)
Nat Brown is one certified geek. He joined Microsoft in 1990 and worked there for nearly 10 years before catching the startup bug. And while Brown may be best known for serving as chief technology officer at iLike and selling his most recent company, rel8tion, to Facebook, his first two startup ventures hit the wall…. Read More
Morning Radar: Sprint to offer unlimited data plan for iPhone 5; PopCap's app; etc.
Sprint is planning to offer unlimited data plans for the iPhone 5 when the device debuts on the wireless carrier's network next month. Bloomberg News, citing unnamed sources familiar with the plans, reports that Sprint would be the only U.S. carrier to offer Apple's popular smartphone with unlimited data. Is that enough for you to… Read More
Seattle entrepreneurs put their best pitch forward at Startup Riot
Seattle startup Chewsy and two dozen other startup companies are getting the chance to present their companies today at Startup Riot, a new event that looks to connect entrepreneurs with investors. Each of the 25 entrepreneurs was given just three minutes to present to the judging panel, which included venture capitalists such as Bill Bryant… Read More
How book stores can compete against Amazon: John Hodgman suggests 'downloading pods'
Amazon.com continues to grab market share from traditional book stores, and just last month Border's bit the dust. But resident expert John Hodgman — appearing Tuesday night on The Daily Show with Jon Stewart — offers some sage advice on how brick-and-mortar bookstores can actually compete. For one, he suggests getting rid of the "old-fashioned"… Read More
Watch a Coldplay or Katy Perry video on Lockerz, and earn rewards along the way
Lockerz, the fast-growing Seattle social networking upstart targeting teens and twenty somethings, has inked a deal with EMI Music to distribute thousands of videos from artists such as Coldplay, Lenny Kravitz, Beastie Boys and more. Of course, you can already watch many of those videos on YouTube or other online channels. But, here's the interesting… Read More
This Seattle woman really, really wants to work at Valve
Valve Software is one of the Seattle area's hottest gaming companies. And Erin Michael Vondrak, a Seattle artist and photographer, really wants to work there. Vondrak composed an original song and video — dubbed Dear Valve: Hire Me — which spells out why she'd be a good fit for the maker of Portal 2 and… Read More

subset: commoncrawl


text:
One of the Most Disgusting Anti-Choice Articles You'll Ever Read
The right wing media are staging another one of their absurd freak-out sessions, this time pretending to be gravely offended by the use of wire coat hangers as a symbol of illegal abortion.
And rarely have I read such a truly despicable example of anti-choice propaganda as Kevin D. Williamson's disgusting attempt to mansplain and deny this bloody history in the National Review: The Symbol of a Lie.
Warning: this is some really sick thinking.
But first a word of praise for the DC Abortion Fund: The group at least has the intellectual integrity to put the word "abortion" right there in the middle of its name — no NARAL-style "choice" euphemisms for this merry band of butchers. Valerie Solanas had her Society for Cutting Up Men, and nobody would be better pleased than I if Cecile Richards et al. embraced their true identity: The Society for Cutting Up Mankind.
Alec Torres noted this exercise in horrifyingly bad taste on the Corner, and the subsequent response on talk radio and elsewhere inspired the DC Abortion Fund to publish an apologia pro carnificibus titled "The Coat Hanger as a Symbol, and Why Abortion Access Matters," which reads in part: "The coat hanger is a symbol of the reproductive justice movement because lack of access to abortion causes women to go to desperate lengths to terminate a pregnancy, similar to those undertaken in the pre-Roe vs. Wade era. At that time, consuming Lysol and household poisons was not uncommon to instigate abortion. Nor was inserting knitting needles, Coke bottles, and — yes — wire coat hangers into their cervices."
That is untrue. It has long been known to be untrue. The wire hanger is indeed a powerful symbol — the symbol of a lie engineered with malice aforethought.
Where's Williamson's evidence that women never used coat hangers to perform abortions? Well, he has none. That's right, he offers not a single piece of evidence for this categorical denial.
And refuting this dishonest, horrible piece of work is incredibly simple. All we have to do is read the history and testimonials from those who were there.
For example, this 2008 essay in the New York Times by retired gynecologist Waldo L. Fielding: Doctor Recalls Abortion Complications Before Roe v. Wade.
I am a retired gynecologist, in my mid-80s. My early formal training in my specialty was spent in New York City, from 1948 to 1953, in two of the city's large municipal hospitals.
There I saw and treated almost every complication of illegal abortion that one could conjure, done either by the patient herself or by an abortionist — often unknowing, unskilled and probably uncaring. Yet the patient never told us who did the work, or where and under what conditions it was performed. She was in dire need of our help to complete the process or, as frequently was the case, to correct what damage might have been done.
The patient also did not explain why she had attempted the abortion, and we did not ask. This was a decision she made for herself, and the reasons were hers alone. Yet this much was clear: The woman had put herself at total risk, and literally did not know whether she would live or die.
This, too, was clear: Her desperate need to terminate a pregnancy was the driving force behind the selection of any method available.
The familiar symbol of illegal abortion is the infamous "coat hanger" — which may be the symbol, but is in no way a myth. In my years in New York, several women arrived with a hanger still in place. Whoever put it in — perhaps the patient herself — found it trapped in the cervix and could not remove it.
We did not have ultrasound, CT scans or any of the now accepted radiology techniques. The woman was placed under anesthesia, and as we removed the metal piece we held our breath, because we could not tell whether the hanger had gone through the uterus into the abdominal cavity. Fortunately, in the cases I saw, it had not.
In a long tradition of shameful right wing propaganda, the National Review has outdone themselves with this one. What the hell is wrong with these people?


text:
The movement of food from mouth to stomach requires normal and coordinated action of the mouth and throat, propulsive waves of the esophagus, and relaxation of the sphincters. A problem with any of these functions can cause difficulty swallowing (dysphagia), regurgitation (the spitting up of food from the esophagus or stomach without nausea or forceful contractions of abdominal muscles), vomiting, or aspiration of food (sucking food into the airways when inhaling).
Achalasia (also called cardiospasm, esophageal aperistalsis, or megaesophagus) is a disorder in which the rhythmic contractions of the esophagus are greatly decreased, the lower esophageal sphincter does not relax normally, and the resting pressure of the lower esophageal sphincter is increased.
Achalasia results from a malfunction of the nerves controlling the rhythmic contractions of the esophagus. The cause of the nerve malfunction is not known, but a viral cause is suspected. Certain tumors may cause achalasia either by directly constricting (narrowing) the lower esophageal sphincter or by infiltrating the nerves of the esophagus. Chagas' disease, which causes the destruction of clusters of nerve cells (autonomic ganglia), may also result in achalasia.
As people age, several changes may affect the ability to swallow. Slightly less saliva is produced. As a result, food is softened (macerated) less well and is drier before it is swallowed. The muscles in the jaws and throat may weaken slightly, making chewing and swallowing less efficient. Also, older people are more likely to have conditions that make chewing and swallowing difficult. For example, they are more likely to have loose teeth or to wear dentures.
With aging, the contractions that move food through the esophagus become weaker. This change is very slight and usually has little effect on moving food to the stomach. But if older people try to eat while lying down or lie down just after eating, food may not easily move to the stomach. If reflux develops, the aging esophagus may be slower to move refluxed stomach acid back into the stomach. Some older people have a hiatus hernia, which may contribute to reflux.
Achalasia may occur at any age but usually begins, almost unnoticed, between the ages of 20 and 60 and then progresses gradually over many months or years. The tight lower esophageal sphincter causes the part of the esophagus above it to enlarge greatly. This enlargement contributes to many of the symptoms. Difficulty swallowing both solids and liquids is the main symptom. Other symptoms may include chest pain; regurgitation of the bland, nonacidic contents of the enlarged esophagus; and coughing at night. Although uncommon, chest pain may occur during swallowing or for no apparent reason. About one third of people who have achalasia regurgitate undigested food while sleeping. They may inhale food into their lungs, which can cause coughing, a lung abscess, infection of the airways, bronchiectasis, or aspiration pneumonia. Undigested food typically remains in the esophagus. Mild to moderate weight loss also occurs. When people have significant weight loss, especially older people whose symptoms of dysphagia developed rapidly, doctors consider and look for a tumor at the gastroesophageal junction (the place where the esophagus connects to the stomach).
X-rays of the esophagus taken while the person is swallowing barium (a barium swallow—see see X-Ray Studies) show an absence of the normal rhythmic waves of muscular contractions (peristalsis). The esophagus is widened, usually only moderately but occasionally to enormous proportions, but is narrow at the lower esophageal sphincter.
Doctors usually also insert a small tube into the esophagus to take pressure measurements of the contractions (esophageal manometry—see see Manometry). Often, doctors examine the esophagus through a flexible viewing tube (esophagoscopy—see see Endoscopy). During an esophagoscopy, the doctor performs a biopsy (removal of tissue samples for examination under a microscope) to make sure the symptoms are not caused by cancer at the lower end of the esophagus.
Achalasia that is caused by cancer at the gastroesophageal junction can be diagnosed by computed tomography (CT) of the chest and abdomen or by an endoscopic ultrasound (a tiny ultrasound probe on the tip of an endoscope that is passed through the mouth into the stomach).
No treatment restores peristalsis. The aim of treatment is to relieve symptoms by decreasing pressure in the lower esophageal sphincter. Nitrates (for example, nitroglycerin Some Trade Names
placed under the tongue before meals) or calcium channel blockers (for example, nifedipine Some Trade Names
) are of limited effectiveness but may delay the need for dilation by helping to relax the sphincter.
Dilation widens the sphincter mechanically—for example, by inflating a large balloon inside it. This procedure helps about 85% of the time, but repeated dilations may be needed. In fewer than 2% of people with achalasia, the esophagus ruptures during the dilation procedure. Esophageal rupture leads to severe inflammation in the chest outside the esophagus (mediastinitis) and, in rare cases, is fatal if not treated appropriately. Immediate surgery is needed to close the rupture in the wall of the esophagus.
As an alternative to mechanical dilation, a doctor may inject botulinum toxin Some Trade Names
into the lower esophageal sphincter. This therapy is as effective as mechanical dilation with balloons. Botulinum toxin Some Trade Names
seems to be successful in providing sustained symptom relief for 70 to 80% of people, but the relief may last only 6 months to 1 year.
If dilation or botulinum toxin Some Trade Names
therapy does not work, a surgical procedure to cut the muscular fibers in the lower esophageal sphincter (myotomy) is usually performed. The procedure can be done with a laparoscope (see see Laparoscopy) or a thoracoscope (see see Thoracoscopy). This procedure is successful about 85% of the time. A procedure (called a fundoplication) to prevent backflow of acid from the stomach (gastroesophageal reflux or GERD) is usually performed during the same surgery. About 15% of people experience episodic backflow of acid (reflux) after surgery.
Esophageal pouches (diverticula) are abnormal protrusions from the esophagus that in rare cases cause swallowing difficulties and regurgitation (the spitting up of food from the esophagus or stomach without nausea or forceful contractions of abdominal muscles).
There are several types of esophageal diverticula. Each has a different cause, but probably all are related to uncoordinated swallowing and muscle relaxation. Most of these diverticula are associated with motility disorders of the esophagus, such as esophageal spasm and achalasia.
A pharyngeal pouch or Zenker's diverticulum is probably caused by an incoordination between movement of food out of the mouth and relaxation of the cricopharyngeal muscle. This diverticulum can fill with food, which may be regurgitated when the person bends over or lies down. This may also cause food to be inhaled into the lungs during sleep, resulting in aspiration pneumonia. Rarely, the pouch enlarges and causes swallowing difficulty and sometimes a swelling in the neck.
A midesophageal pouch or traction diverticulum is caused by traction from inflamed lesions located in the chest outside the esophagus (mediastinum) or, secondarily, by esophageal movement (motility) disorders. A traction diverticulum rarely causes symptoms, but the underlying disorder may.
An epiphrenic pouch or diverticulum occurs just above the diaphragm and usually accompanies a motility disorder (such as achalasia or esophageal spasm). An epiphrenic diverticulum rarely causes symptoms, but the underlying disorder may.
All diverticula can be diagnosed with a videotaped barium swallow.
Treatment is not usually needed. If symptoms are severe, however, the pouch can be removed surgically. Diverticula associated with motility disorders require treatment of the underlying disorder. For example, if a Zenker's diverticulum is caused by an abnormally functioning cricopharyngeal muscle, a doctor can cut the muscle (myotomy) when removing the Zenker's diverticulum. When esophageal spasm or achalasia is present, treatment of sphincter tightness may be needed.
Esophageal spasm (also called spastic pseudodiverticulosis, rosary bead or corkscrew esophagus, or symptomatic diffuse esophageal spasm) is a disorder of the propulsive movements (peristalsis) of the esophagus.
In this disorder, the normal propulsive contractions that move food through the esophagus are replaced periodically by nonpropulsive contractions or excessive muscular contractions (hyperdynamia). In 30% of people with this disorder, the lower esophageal sphincter opens and closes abnormally. The exact cause is not known but is suspected to be a nerve defect.
Muscle spasms throughout the esophagus typically are felt as chest pain under the breastbone coinciding with difficulty in swallowing liquids (especially those that are very hot or cold) and solids. Pain also occurs at night and may be severe enough to awaken a person. Esophageal spasm also may cause severe pain without swallowing difficulty. This pain, often described as a squeezing pain under the breastbone, may accompany exercise or exertion, making it difficult for a doctor to distinguish it from angina (chest pain stemming from heart disease). Over many years, this disorder may evolve into achalasia (see see Achalasia), a disorder in which the rhythmic contractions of the esophagus are greatly decreased. Some people have symptoms that combine those of achalasia and diffuse esophageal spasm. One such combination of symptoms has been called vigorous achalasia. It features both food retention, which can lead to aspiration, as well as severe chest pain from diffuse esophageal spasm.
X-rays taken while the person swallows a barium drink (a barium swallow—see see X-Ray Studies) may show that liquid barium does not move normally down the esophagus and that contractions of the esophageal wall are uncoordinated and do not propel the barium. Pressure measurements by manometry (a test in which a tube placed in the esophagus measures the pressure of contractions—see see Manometry) provide the most sensitive and detailed analysis of the spasms.
Esophageal spasm is often difficult to treat. Calcium channel blockers such as nifedipine Some Trade Names
may relieve the symptoms by relaxing the muscles of the esophagus. Sometimes, injecting botulinum toxin Some Trade Names
into the muscles of the esophagus is helpful. Other drugs such as nitroglycerin Some Trade Names
, long-acting nitrates, and drugs with anticholinergic effects (such as dicyclomine Some Trade Names
) are less successful. Sometimes people need strong analgesics. In many cases, a narrowing is treated by inflating a balloon inside the esophagus or by inserting bougies (progressively larger dilators) to dilate the esophagus. In rare cases, if other less radical forms of treatment are not effective, a surgeon may cut the muscle layer along the full length of the esophagus (surgical myotomy).
Propulsion disorders of the throat can cause trouble moving food from the upper part of the throat into the esophagus. Such problems occur most often in people who have disorders of the throat muscles or the nerves that serve them. The most common cause is stroke. Dermatomyositis, scleroderma, myasthenia gravis, muscular dystrophy, polio, pseudobulbar palsy, Parkinson's disease, and amyotrophic lateral sclerosis (Lou Gehrig's disease) all can affect the throat muscles or nerves. Difficulty swallowing may also result from the use of a phenothiazine (a class of antipsychotic drug), because these drugs can impair the normal function of the throat muscles. People with a propulsion disorder of the throat often regurgitate food through the back of the nose or inhale it into the windpipe (trachea), which causes them to cough.
In cricopharyngeal incoordination, the upper esophageal sphincter (cricopharyngeal muscle) remains closed, or it opens in an uncoordinated way. An abnormally functioning sphincter may allow food to repeatedly enter the windpipe and lungs, which may lead to recurring lung infections and eventually to chronic lung disease. A surgeon can cut the sphincter so that it is permanently relaxed. If left untreated, the condition may lead to the formation of a diverticulum (see see Esophageal Pouches), a pouch formed when the lining of the esophagus pushes outward and backward through the cricopharyngeal muscle.
Last full review/revision October 2007 by Michael C. DiMarino, MD
amyotrophic lateral sclerosis
computed tomography
cricopharyngeal incoordination
gastroesophageal reflux
muscular dystrophy
myasthenia gravis
Zenker's diverticulum
Previous: Overview of the Esophagus
Infection of the Esophagus


text:
Funding opportunities
Dopaminergic Differentiation of Human Embryonic Stem Cells
Funding Type:
Comprehensive Grant
Grant Number:
Funds requested:
$2 937 237
Funding Recommendations:
Not recommended
Grant approved:
Public Abstract:
Parkinson's disease is a devastating neurodegenerative disorder for which there is no cure. Several foundations including the Michael J. Fox Foundation have identified cell therapy as one possible avenue for research as cell therapy using fetal tissue-derived cells has been shown to be successful in multiple transplant studies. Work in the field had been limited however, due to the limited availability of cells for transplantation as cells from 6-10 human fetuses of 6-10 weeks post-conception is required for a single patient. Human embryonic stem cells offer a potentially unlimited source of the right kind of cell required for cell replacement therapy due to their remarkable ability to self-renew (they can divide indefinitely in culture) and to be trained to become any cell type of the body. In this proposal we seek to understand how embryonic stem cells differentiate into authentic dopaminergic neurons using a culture method that we have tested. We believe that this differentiation can be broken into several stages each of which is regulated by growth factors and other molecules. We also believe that these stages can be understood by selecting cells at each stage and comparing their properties using molecular tools and by examining their behavior after transplanting the cells into a mouse disease model. In addition, we will need to develop a noninvasive method of following cells after transplantation and we propose to develop a TH-ferritin (that can be detected by magnetic resonance imaging, MRI) reporter line based on previous successful work in mice to monitor dopaminergic neurons in animal models and possibly in future clinical trials. We believe that these experiments are critical to enhancing our understanding of the disease and providing the tools that will be necessary to move cell therapy to the clinic.
Statement of Benefit to California:
We have proposed three aims in this proposal to develop markers that can distinguish stages in the process of development, to develop reporter lines that allow us to isolates cells at each specific stage of differentiation, and to identify the optimal cells for therapy using animal models that mimic the human disease. We have also proposed to assess the long-term integration and differentiation of transplanted cells using a magnetic resonance imaging (MRI)-based labeling system. We believe these experiments not only provide a blueprint for moving towards the clinic for Parkinson's disease sufferers but also a generalized plan for how cell therapy will need to be developed for treatment of multiple disorders including motor neuron diseases and spinal cord injury. The tools and reagents that we develop will be made widely available to Californian researchers and we will select California-based companies for commercialization of such therapies. We will hope that it is California-based physicians who will be at the forefront of developing this promising avenue of research. We expect that the money expended on this research will benefit the Californian research community, and the tools and reagents we develop will help accelerate the research of our colleagues in both California and worldwide.
Review Summary:
SYNOPSIS: The Principal Investigator (PI) is an Assistant Professor at the Buck Institute of Age Research and the focus of this proposal is on dopaminergic differentiation of human embryonic stem cells (hESCs). He proposes to assess the process and stages of dopaminergic differentiation in hESCs through markers, to sort the cells at different stages of differentiation, to develop stage-specific reporter lines, to identify novel regulators and markers using global gene expression profiling, and then to transplant the cells at different stages in an MPTP mouse model of Parkinson's disease. IMPACT & SIGNIFICANCE: Many cell transplant studies have been carried out in Parkinson's disease (PD), including transplantation of human fetal dopaminergic cells into brains of people with the disease. The results of the transplants have been quite controversial and it is widely acknowledged that the cells may accelerate the onset of on-off syndrome. Goldman, et al. (2006) reported successful use of dopaminergic hESCs to treat a rat 6-OHDA model of PD but also observed continued proliferation of the cells and possible tumorigenic potential of such growth. It is not clear that this study will contribute very much more to the field. It is possible that transplanting hESC's at various stages of differentiation towards dopaminergic cells may avoid some of the problems of transplanting fetal cells in the brain. However, it is not clear why this should be so. That said, hESCs represent a potential cell source for cell replacement therapy of PD. The selective degeneration of a specific cell type, the A9 ventral mesencephalic dopaminergic (DA) neurons, being the direct cause of this common and devastating disease, makes PD an important and ideal target disease for stem cell therapy. With the long-term goal to assess the potential of hESC-derived DA neurons for cell replacement therapy of PD, this application proposes to achieve a comprehensive understanding of human DA development, to establish optimal techniques to enrich appropriate matured DA cell populations, and to assess functional and behavior improvements after transplantation of hESC-derived DA cells in PD animal models. Thus, this proposal in general contains logical steps and approaches for successful hESC-based cell therapy of PD and is expected to significantly advance the field and to establish important bases for therapeutic application. PD and DA neuron differentiation are important areas of research. FACS based on cell surface markers is a potentially powerful strategy to make cell DA neuron grafting safer. Technology development is quite strong in this proposal with the help of Dr. Rao. However, there are concerns that the key limitations of current hESC DA neuron approaches are not addressed. QUALITY OF THE RESEARCH PLAN: The research plan appears to be quite feasible and the applicant appears to quite capable of producing hESC reporter lines by homologous recombination and doing large-scale genomic analysis of the hESC reporter lines. The techniques are well-described. The applicant discusses the use of cyclosporin to prevent immune-rejection of the transplanted human cells started one day before transplantation. He will use ferritin-labeled cells (by the way, the ferritin may be taken up by other cells and therefore is not a particularly long-term marker). He also shows preliminary data on hESC cultures and cells at different stages of differentiation towards dopaminergic cells. The proposed work is divided into 3 specific aims. In aim 1, the PI proposes to assess the process/stages of DA differentiation in hESCs by monitoring specific marker expression. First, the PI will examine gene expression associated with DA development at different stages of hESC culture and differentiation using PA6-conditioned medium and other signals, by several techniques such as immunocytochemistry, RT-PCR, qPCR, and focused microarrays. This is a tremendous effort to provide a snapshot of DA-associated gene profiles at different stages. For instance, the PI plans to perform microarray analysis for more than 15 time points along the 30 day protocol (Table 1). Second, the PI will assess the maturation of the DA neurons by electrophysiology and DA production. HPLC analyses will be used to measure DA and its metabolites at different stages of development. Thirdly, the PI will attempt to purify and/or enrich DA cell populations by FACS using cell surface markers such as PSA-NCAM, TrkB, and p75. Using each antibody, FACS will be used to isolate differentiating cells and their viability determined. It is highly important to define a core set of stage-specific markers for our comprehensive understanding of DA differentiation. Toward this goal, the proposed experiments will provide useful information. However, there are some serious flaws and concerns in the proposed approaches according to one reviewer. First, although this reviewer absolutely agrees with the PI that a comprehensive understanding of human DA development is critical for successful cell replacement therapy of PD, s/he is not convinced that DA differentiation by PA6-CM indeed represents the natural course of differentiation. Second, considering the heterogeneous cell populations at each stage, the tremendous effort described in experiment 1.1. (including more than 15 time points of microarray analyses) is not well-justified. Ironically, the PI mentions that "further purification of cells will be required prior to any transplant or gene expression studies" in experiment 1.3. In fact, this reviewer is doubtful if this extensive effort using very heterogeneous cell populations at multiple time points could provide additional insights about stage-specific markers of DA differentiation. In aim 2, the PI will develop stage-specific reporter hESC lines using site-specific integration and homologous recombination. The PI's rationale is to determine a temporal profile of DA differentiation and to identify a core set of markers, and to determine the optimal stages to sort cells. In contrast, it seems that the PI already planned to use Sox1, Ta1, and TH genes to make reporter lines specific for neural stem cells, neuronal progenitors, and mature DA neurons, respectively. The PI will also generate TH-ferritin reporter hESC line for noninvasive investigation by MRI. These are very innovative ideas and will generate very useful resources for stem cell research in relation to PD. A reviewer points out that Aim 2 is not well focused because competing strategies are used to achieve the same goal. Stable genetic modification in hESC are difficult to achieve, particularly via homologous recombination. It is unclear why the applicant proposes two strategies for the same goal instead of focusing on the most promising approach. The use of interesting technology such as illumina bead sorting for gene expression analysis is proposed. However, there is no clear discussion on why this technology will be superior for the biological problem in question. The preliminary data is considered quite strong in some parts of the grant; but preliminary data on cell surface marker sorting need to be presented in order to validate that approach. The preliminary data on DA neuron differentiation are limited to TH expression by immunocytochemistry. TH is strongly expressed in peripheral neurons and could be a confounding factor. It is essential to show co-labeling of TH with midbrain markers. Gene expression analysis cannot compensate for the lack of co-labeling studies. Some of the markers proposed such as p75 will likely pull out neural crest stem progenitors in addition to any other presumptive ventral neural cell types. STRENGTHS: The idea of transplanting hNSCs at different stages of differentiation into animal PD models is a good one. The applicant shows preliminary data that they can indeed get stage-specific reporter cells, purify them, and transplant them. A number of very innovative technologies are proposed, and the experiments may yield some interesting data. The strengths of this application include the expertise, productivity, and enthusiasm of the PI and establishment of excellent collaborators. Dr. Zeng is an extremely talented and promising young scientist with strong background and expertise in hESC studies. Many of Dr. Zeng's publications are directly related to characterization of differentiation properties of several lines of hESC lines using molecular, cellular, microarray, and transplantation studies. The PI's collaborators, Dr. Rao and Dr. Andersen, are excellent scientists and leaders in their respective field of research (stem cell research and PD research). Their collaboration will significantly strengthen the feasibility of the proposed experiments. Another strength of this application is the significance and potential impact of the research area for the future treatment of one of the most common neurodegenerative disorders, PD. Assessment and comprehensive understanding of the process/stages of DA differentiation of hESCs are prerequisite for advancing cell replacement therapy of PD. The PI proposes several logical approaches that will likely generate very useful information for the stem cell field WEAKNESSES: It is not clear that this approach will solve the clinical problem of Parkinson's disease where the beneficial effects of transplanting fetal dopaminergic cells have been quite controversial and it is not clear that this experiment will yield results that are much more informative or successful than other studies. The enthusiasm of a reviewer is somewhat dampened by several weaknesses of this proposal. First of all, there is a flaw in the design of experiment 1.1 in aim 1. The PI plans to perform microarray analyses on more than 15 time points during in vitro DA differentiation of the BG01 hESC line. They will have to contend with highly heterogeneous cell populations and it is doubtful that meaningful candidates can be identified from this extensive effort. It is very likely that hundreds, if not thousands, of possible candidates will be identified at different stages. In fact, the PI states that "further purification of cells will be required prior to any transplant or gene expression studies" in experiment 1.3. Second, it was not made clear in the application why the PI proposes to establish hESC reporter lines by phi C31 integrase while this method is not yet established. Instead, homologous recombination seems to be more promising as judged by the preliminary results. Third, reviewers generally agree that the transplantation studies in aim 3 are poorly designed without appropriate and systematic considerations. Behavioral paradigms are not specific and their interpretations and statistical analyses are not carefully designed. Overall, although the proposed experiments are important and worthwhile in general, they lack clear rationale and logical designs in some parts. Additional weaknesses were identified by reviewers. There is not enough emphasis on the need to combine markers for proper midbrain DA neuron identification. No details on sorting strategy are provided which makes it impossible for the reviewer to properly assess the merit of this approach. No justification is provided as to why knock-in and site-specific integration is used for the very same application. There is also confusion about the type of fluorophores to be used (inconsistently listed throughout the application). Also, there is limited justification for some of the technology to be used. Discussion of appropriate controls is weak; NT2 derived DA neurons or human fetal DA neurons are of limited use if they are not matched for developmental stages. The in vivo study does not address the main current limitation in hES DA neuron grafting which is poor survival and maintenance of phenotype; without evidence of surviving TH cells in vivo, the TH-ferritin approach appears premature. Finally, no physiologist is identified for carrying out these studies Reviewers recommended several changes to the proposal. There should be a more in depth discussion of the controversial role of dopaminergic cell transplants for PD and why this approach with hESC would circumvent the problems associated with fetal dopaminergic cell transplants. Aim 1 should be better developed with clear description and rationale of any strategies proposed that may improve DA neuron differentiation. Some preliminary data are required on surface marker/ ACS approach. Aim 2 should be focused on one technology platform (e.g. site directed integration) so that the efforts can be better focused. Aim 3 needs to take into account the current limitations of hESC DA neuron grafts and the use of Ferritin reporter should be proposed only if appropriate preliminary data on TH survival are available. DISCUSSION: Reviewers enthusiastically agreed that a general strength of this application is the PI, who has strong potential and many good recent publications (if not in the highest impact journals). The proposal seems generally logical and reasonable, but when examined carefully, there are many deep concerns about the study design. Additional strengths mentioned included good collaborators, and a good discussion of the differences with the animal models. There is an issue with the poor characterization of the cells and there is no preliminary data on the markers used to purify the cells, and one marker (p75) is a classical neural crest marker but is not purely for DA cells. The methods proposed to establish reporter lines, phi C31 integrase and homologous recombination, are both technically challenging with the former even less established than the latter.


text: Winter Wonderland First Birthday Party A Magical Winter Wonderland First Birthday Party by Katharine Stahl 2/08/13 27 Reactions 246 Shares Print 223 12 11 0 0 "Even though we live in Los Angeles, and it's not technically freezing over here, I still loved the idea of making a Winter wonderland-themed first birthday party for Ariella, our little Winter baby," says Emily Klaparda, mom of two and the creative mind behind Emily K Paperie. "I didn't want to re-create those typical Winter parties with penguins, polar bears, and igloos. Instead, I had the idea in my head for a rustic, shabby-chic party that had the Winter feel, but was a bit more girlie and sophisticated — just like our little girl!" With gorgeous and cozy details including Manzanita branch centerpieces, glittery snowflakes, a sweet hot cocoa bar, and lots of sparkly silver and pink trees, this party makes us wish it was Winter all year round (minus the freezing temperatures, of course). Source: Emily K Paperie Baby, It's Cold Outside What's Your Reaction? 0 0 0 0 0 0 Emily created signs for the party herself on Shugabee Lane's vintage framed chalkboards. Birthday girl Ariella wore a lace crown from Sweet Louise Boutique. Source: Emily K Paperie 1 / 17 Snowflakes Everywhere What's Your Reaction? 0 0 0 0 0 0 Emily designed the beautiful invitation, introducing the Winter wonderland theme. The snowflake cake was created by Sweet E's Bake Shop. Source: Emily K Paperie 2 / 17 Flowers and Lace What's Your Reaction? 0 0 0 0 0 0 Emily created the beautiful flower arrangements herself. Source: Emily K Paperie 3 / 17 Branches and Glitter What's Your Reaction? 0 0 0 0 0 0 "I found an online tutorial about setting the Manzanita branches in Plaster of Paris," Emily said. "I followed the steps and was very pleased with the end result! I glued dried flowers onto the branches to complete the look." Source: Emily K Paperie 4 / 17 Winter White What's Your Reaction? 0 0 0 0 0 0 The front-door wreath was created by Dandelion Crafting Co., while Emily scored the letter "A" from Etsy's This Makes Me Happy. The snowflake balloons were a find from Single Source Party Rentals. Source: Emily K Paperie 6 / 17 Lovely Mantel What's Your Reaction? 0 0 0 0 0 0 "I was especially pleased with how the mantel decor turned out," Emily said. "It was a great backdrop for the birthday girl and her cake smash. I purchased some bulk burlap and designed the 'swag' and then tied it up. I found the pink trees at Michael's, and then added the floral centerpieces and the birthday banner from Prop Shop Boutique." Source: Emily K Paperie 6 / 17 Trees, Please What's Your Reaction? 0 0 0 0 0 0 Bottle brush trees from Love It Shabby are one of our favorite party elements. So beautiful, right? Source: Emily K Paperie 7 / 17 Sweet Stuff What's Your Reaction? 0 0 0 0 0 0 A closer look at the Prop Shop Boutique banner and cupcakes with fondant toppers from Two Sweet Cakes. Source: Emily K Paperie 8 / 17 Delicious Details What's Your Reaction? 0 0 0 0 0 0 White chocolate Oreos from Sweet E's Bake Shop were displayed with other Winter-themed desserts. Source: Emily K Paperie 9 / 17 Snowy Weather What's Your Reaction? 0 0 0 0 0 0 Cake pops from Sweet E's Bake Shop and heart-stamped wooden spoons from Etsy's Angie Hearts Jared. Source: Emily K Paperie 11 / 17 S'More Bar What's Your Reaction? 0 0 0 0 0 0 A s'more bar made eating fun and interactive for Ariella's guests. Source: Emily K Paperie 11 / 17 Hot Cocoa Bar What's Your Reaction? 0 0 0 0 0 0 "This was a real hit with our guests, who all loved the gourmet hot cocoa and unique toppings — especially the handmade marshmallows!" Emily said. "Perfect for a chilly Winter party!" Emily gathered the homemade marshmallows from One More Bite, Mia Mallows, and Guimauve Confections. Source: Emily K Paperie 12 / 17 Warm Up! What's Your Reaction? 0 0 0 0 0 0 Guests enjoying the hot cocoa bar, and a chalkboard sign, created by Emily. Source: Emily K Paperie 13 / 17 Food Table What's Your Reaction? 0 0 0 0 0 0 Whole Foods catered the savory offerings. Source: Emily K Paperie 14 / 17 Soup and Snowy Shooters What's Your Reaction? 0 0 0 0 0 0 Grilled cheese and tomato soup shooters, next to milk designed to appeal to guests of all ages. Source: Emily K Paperie 15 / 17 Grilled Cheese and Tomato Soup What's Your Reaction? 0 0 0 0 0 0 "I saw these at a party awhile back and thought they would be a great addition to Ariella's party!" Emily said of the grilled cheese and tomato soup shooters. "They were a great, warm comfort food for a cold Winter day! Guests loved them and even asked for refills." Source: Emily K Paperie 16 / 17 Birthday Girl What's Your Reaction? 0 0 0 0 0 0 One-year-old Ariella was the guest of honor at this beautiful Winter wonderland party. Source: Emily K Paperie 17 / 17 223 12 11 0 0 Back to Homepage Comments Read More Food And FunSnowflakesFirst Birthday PartiesKid PartiesWinter Food and Fun 66 Fabulous and Unique Birthday Cakes For Baby and Tot 21 hours ago 25 Cakes For Your Little Prince or Princess 3 days ago 15 Fun Food Ideas Your Kids Can Make on Their Own 4 days ago 10 Tricks For Organizing Your Kids' Craft Area 4 days ago 51 of the Best Birthday Party Ideas For Girls 5 days ago Christina Aguilera's a Mom Again — Did You See Her Baby Shower Cake!? 6 days ago What's Your Reaction? 0 0 0 0 0 0 0Reactions Link Love Mom forced to stop feeding baby homemade formula — BabyCenter Blog The easiest ways to save on organic food — The Stir A look inside Fergie's second baby shower — Celebrity Baby Scoop Danielle Jonas debuts her baby bump — People Moms & Babies 7 light and fresh recipes to take you through Summer — FitPregnancy Does where you live affect your risk for PPD? — The Bump Where to find the best deals on school supplies — Real Simple Now this is how you announce a pregnancy! — HuffPost Parents


text:
You save:
Free Shipping
Free Shipping with use of RadioShack credit card
Add lighting to your 12-volt project with an LED strip light. This flexible, water resistant strip light will highlight any installation. Available in multiple colors and lengths
Online prices and product selection may vary from our retail stores. All prices and offers are subject to change. Copyright RadioShack Corporation 2014. All rights reserved.


text:
Business Centre
The latest business news, reviews, features and whitepapers
Enterprise initiatives designed to facilitate mobility can deliver important benefits to your organisation. This paper suggests best practices for selecting the right devices and implementing the most effective management solutions • Employees need devices that match preferences while meeting business and IT requirements • You must determine which devices to allow/recommend as part of a choose-your-own-device (CYOD) or member purchase plan (MPP) • IT groups need a solution that can support new technologies that may emerge in the future Download whitepaper »
In the current market, there is an increasing demand for unbiased information about Enterprise Mobility Management (EMM) solutions. This whitepaper focuses on relevant solutions with an extensive overview of features for comparison. • The paper discusses Application and Desktop Delivery in the mobile world • Details challenges of the consumerisation of IT and BYO in tomorrow's workspace • Explains the different Mobile Managements concepts, including the pros and cons Download whitepaper »
With EmblemHealth's company headquarters flooded after Hurricane Sandy in 2012, this paper explains how a mobile laptop solution enabled displaced employees to work remotely and maintain productivity. • Hurricane Sandy's surge swamped the building's sublevels, where its mechanical and electrical systems reside, rendering it uninhabitable for three months • Employees who were working remotely could access the company's intranet to get updates as needed • Corporate notebooks were also essential to mobile workers such as visiting nurses and health coaches Download whitepaper »
The latest business news, reviews, features and whitepapers
Visit our shop for the best deals on hot products
Watch our video news and reviews from around the world
Comprehensive buying guides, features, and step-by-step articles
First impression on unpacking the Q702 test unit was the solid feel and clean, minimalist styling.
For work use, Microsoft Word and Excel programs pre-installed on the device are adequate for preparing short documents.
The Fujitsu LifeBook UH574 allowed for great mobility without being obnoxiously heavy or clunky. Its twelve hours of battery life did not disappoint.
The screen was particularly good. It is bright and visible from most angles, however heat is an issue, particularly around the Windows button on the front, and on the back where the battery housing is located.
My first impression after unboxing the Q702 is that it is a nice looking unit. Styling is somewhat minimalist but very effective. The tablet part, once detached, has a nice weight, and no buttons or switches are located in awkward or intrusive positions.


text:
added to your cart.
added to wishlist.
added to compare products.
Skip to Main Content »
Move your mouse over image or click to enlarge
Item# : MMP-6539R
Add Your Review
Out of Stock
"Everything will be alright in the end. If it is not alright, it is not the end." This handmade ceramic desk plaque echoes the universal fact that nothing we experience, including doubt and distress, can last forever. Cream base with brown lettering. Measures 5 x 2.5 inches, weighs about 0.5 pounds. Includes copper wire for easy hanging or standing.Handmade in the USA: skillfully created by artisans using natural materials. No two pieces are exactly alike. Variations in color, shape and size may be present.


text:
Authorities in High Point, NC, have canceled an Amber Alert for an abducted girl.
The alert was issued for 17-month-old Shylin Olanda Neal around 1 a.m. Friday.
High Point police said she was last seen outside a Food Lion on Brian James Place in a white 2003 Chevrolet Suburban with North Carolina License plate BJX1565 with an "I love wake boarding" sticker on the back.
They said that car was stolen by an unknown abductor.
The alert was canceled later Friday morning after High Point police said she was found safe along with the SUV.
According to WGHP-TV, she was found safe about 8:30 a.m. when a citizen spotted the SUV along Englewood Street in Greensboro.
WGHP reported that the child was left inside the vehicle for about three to five minutes while her godfather ran inside the store.
No other details have been released about if any arrests have been made in the case.
Copyright 2013 FOX Carolina (Meredith Corporation). All rights reserved.


text:
Best Deals
Customers Also Bought
Product Description
- This adapter at home or at work makes power access convenient and tailored to your needs
- Easy to use
- OCP, OVP, OTP, SCP (Over-current, over voltage, temperature, short circuit ) protection
- Also keep one in your carrying case at all times and never be without a adapter when you travel
- It enables you to keep your battery fully charged when you really need it most on the road
- This is a non-OEM product
- Type: AC-DC
- Power: 65W
- Input : 100-240V (AC 50~60Hz)
- Output Current: 19.5V/3.34A
- Working Temperature: 0-40°C
- Storage Temperature: 15~50°C
- Storage Humidity: 5~95%
- Working Humidity: 10~90%
- Compatible with: DELL Inspiron 6000 600m 630m 640m
- DC Interface Size: 7.4 x 5.0 x 12mm / 0.29 x 0.20 x 0.47in (L x W x H)
- Size: 147 x 61 x 35mm / 5.8 x 2.4 x 1.4in (L x W x H)
- Laptop AC Power Supply Adapter for DELL 6000 600m 630m 640m
How to Test a Laptop Power Adapter:
- Laptops are powered either by their batteries or by connecting to a power adapter and plugging the adapter into the wall. When using an adapter, the adapter is supplying power to the laptop and charging the battery at the same time. Laptop power adapters, also called "AC adapters," are typically small boxes with two cords coming out of them; one end connects to the laptop, and the other end plugs into a power outlet on a wall or a power strip. You can test the adapter to see if it is generating power for the laptop
- Plug the adapter into the wall outlet or power strip. If using a power strip, make sure the strip is on. Most laptop power adapters have a small LED light; the light will illuminate when power is being transferred from the outlet to the adapter. If the light illuminates, chances are you have sufficient power to supply your laptop
- Wiggle the power cord if the LED light did not initially illuminate. If the light turns on and off when you wiggle it, you might have a loose cord in the adapter, or the outlet could be faulty
- Plug the adapter into another power outlet, preferably a different circuit in another room, if the LED light did not illuminate the first time. If it lights up from the second outlet, the first outlet either does not work or other devices were using too much power on the initial circuit
- Connect the other end of the adapter to the laptop and turn the laptop on. When the computer starts up, you should see an indication of how the laptop is being powered in the lower right-hand corner. If you see a battery icon, then the laptop is being powered by the battery, not the adapter. Make sure both ends of the adapter are securely connected. If they are, and if the LED light on the adapter is lit, then you likely have a faulty wire that is not properly connecting to your computer. If you see a plug or adapter icon, then the laptop is successfully being powered by the adapter. If the LED light on the adapter is not lit, then the bulb is out, but the adapter is still working
Tips & Warnings:
- Different adapters supply different levels of electrical power; some adapters are too strong or not strong enough for other laptops. Only use the power adapter that came with your laptop. If it stops working or is lost, order another from the same manufacturer as your laptop; do not use a different brand. Be sure to verify that the new adapter coincides with your laptop model before ordering the new one
- Use caution around electrical outlets. Do not have touch plugs with wet hands or have water nearby, and keep small children away from the outlets. Never insert anything into the outlets other than the adapter plug
Package Included:
- 1 x Laptop AC Power Supply Adapter for DELL Inspiron 6000 600m 630m 640m
If the order has not been shipped; please contact DinoDirect Live Support for order cancellation.You will get your refund within 24 hours.
If the order has already been shipped; you may return the item to us postmarked within 30 calendar days of delivery. Before that, please contact our Live Support to inform that.
In that case we will refund you the payment excluding actual shipping fees already incurred.
If the item is defective, please contact DinoDirect Live Support and send an email to us at email@example.com attached an image or video file clearly showing the defect of the product. And we will give you a response within 24 hours whether we will resend the item or refund the payment to you for compensation.
For customization, please consider carefully before ordering.Because we do not accept return and replacement.
Quantity Range
|Unit Price
|Enter Wholesale Channel>>
Professional Design
DINODIRECT has a team of professional designers, who are all experienced in European and Western design and lead the latest trends in Europe and the United States wedding market design. Our designers are also experienced in customizing dresses; we can do whatever you ask to do on your dresses like Lace and beading. You can tell us your ideas on your dresses and we would like to help you make your ideal wedding dress. We will give you the design in 48 hours with all the unique and charming details.
100% Made to Measure Dresses
DINODIRECT.Produces tailor-made dresses to suit your needs. We ensure that all dresses are 100% handmade. Once you provide the accurate measurement of the details (See our measurement guide) to us, we will immediately start working on your wonderful, unique wedding dress.
Fine Quality
DINODIRECT.com'sdresses are made of best material. DINODIRECT hires only the best dressmakers and experienced designers in china, who use the finest production techniques to create your dress.
The Best Price
DINODIRECT.com's prices are a lot lower than retail prices. With the decades experience in wedding industry, we can offer you all kinds of dresses with the most reasonable prices. Our price varies from US$100-US$800 for some of the highest quality dresses found online.
Speedy Delivery Times
DINODIRECT will ship out your dress as soon as possible. It takes you about 30 days from the day you place your order to the day you receive it.
About DinoDirect.com:
Q: Are the dresses made by your own factory?
A: DinoDirect has our own factory with over five years' experience in making dresses.
Q:Is the price on your website real price, why it is much cheaper than market price?
A:Dinodirect manage our business with lower overhead than a retail store or a company. We needn't employ many different departments, and needn't pay the much fees for advertising, renting a store, asking other team for alteration etc. We are the one of largest professional wedding factory in China, and our clients can order dresses from our factory directly. Since we do not have to accommodate for these costs, we can pass the savings onto our customers.
Q: How to make sure the quality?
A:DINODIRECT.com's dresses are made of best material. DINODIRECT hires only the best dressmakers and experienced designers in china, who use the finest production techniques to create your dress.We ensure that all dresses are 100% handmade.
Buy Dresses:
Q:How do I place my order in this website?
A:You can view the catalog in our website. If you find anything you want to buy just take it to shopping cart and then go check out.
Q:How do I use the search option of your site?
A: You can search by color, by designer, by occasion, by dress type, by price, or by style number. You can search all those categories at once and even sort by price and popularity once you've filtered your dresses.
Q:Can you customize the size of my dress?
A:Absolutely – if our standard sizes don't work for you, we can make you a custom-sized dress for free,When you ordering, you'll need to check the tab for "Custom size",then fill in your size. Getting professionally measured is best, but if you want to do it yourself be sure to read our measuring guide first. Avoid common mistakes by doing the following:
1.Ask someone else to measure for you
2.Take measurements while wearing undergarments similar to what you'll wear with the dress
3.Provide a bust measurement from the fullest part of your chest, not your bra size!
Q:Can I order this dress in a different style/fabric/length?
A: Of course you can. You can click" Custom Size" and then leave your request in "Item Remarks". We will make your dress by your request.
PS: Some dresses do not have "Item Remarks" Tag.
Q: If I buy 2 or more dresses at the same time, do I need to pay postage for twice or more?
A: If you order 2 or more dresses including accessories from our website and we can send them together to same address, you just need to pay for postage once.
Q: How will my item (s) be shipped?
A: All shipping information is outlined in our shipping page.
Q: How long is the delivery time?
A: Usually it takes 7-15 days to make wedding dresses and 1-2 days to check your dresses. The shipping time also differs in shipping methods. The fastest shipping - expedited shipping takes 5-6 days for the shipment. The standard shipping takes 5-9 days. The super saver shipping takes 15-20 days for the shipment. The sooner you place an order, the sooner we start preparing your dress. Thank you.
Q: Will my dress exactly match the color I see on your website?
A: There might be a slight difference between the actual dress's color and what you see in the photo depending on your computer monitor's display settings.
Q:Can I make changes to my order after I have paid for my order?
A:You can change your order within 48 hours after you have paid your order, by contacting DinoDirect Live Support and send an email to us at firstname.lastname@example.com, if more than 48 hours, we could not change your order any more.
Ensuring your order number is included.
Q: What should I do if the size doesn't fit me perfectly?
A:If your size fluctuates or you want to modify the dress slightly, we always leave a few inches in the seams so that your local tailor can easily make alterations as needed. Dresses generally have a margin of 1 inches on either side of a seam. Flower detailing, beading or belts/ribbons may limit ability to let a dress out.
Q:How do I upgrade my shipping?
A: Simply respond to your confirmation email with the method you would like to change it to.
Q:How to Preserve my Dress?
A: In order to prevent the dress distorting and yellowing, you can choose professional dust bag to restore.
Q:What should I do if I have forgotten my password or my password does not work?
A: In our register page, please click "forgot my password", you will receive an email. There will be a link in the email, click that link and reset your password. If you don't receive the email, please check it again in your spam mail. Please contact with our Customer Service online if you don't receive it indeed.


text:
Mandriva Cooker : The inside Man
Many things happen lately on Mandriva Cooker :
*KDE 3.5.2 : After having releasing koffice 1.5 with maestria, Laurent begins to put KDE 3.5.2 in cooker.
*Gnome 2.14.1 : Still on the bleeding edge front, after his holiday in Mexico, Fred begins to put Gnome 2.14.1 in cooker.
*Mono : Another good news is the moving of mono in main. At the same time beagle support in Nautilus has been activated. Maybe Beagle will become the standard for documents indexing in Mandriva ?
- Login or register to post comments
- Printer-friendly version
- PDF version
More in Tux Machines
Testing Fedora 21 fitness for world population with Internationalization
Fedora is a global Linux distribution, as soon as we say the word "Global", immediately internationalization (i18n) and localization(l10n) become a utmost important part of the distribution.
Ondemand vs. Performance CPU Governing For AMD FX CPUs On Linux 3.17
In the tests shared yesterday of looking at the AMD FX-9590 CPU on Linux and other CPU benchmarks from this weekend, some Phoronix readers raised concerns about the CPU scaling governor differences between the AMD and Intel hardware. The AMD FX CPUs continue to use the CPUfreq driver by default to handle their scaling while modern Intel CPUs have the new Intel P-State driver. Beyond the Intel-specific P-State vs. CPUfreq, the AMD CPUs generally default to using the "ondemand" governor while with Intel desktop CPUs on P-State it generally ends up with the "performance" mode. Some Phoronix readers found performance vs. ondemand differences to be unfair, but for AMD FX CPUs, there isn't much of a difference in our common CPU torture test benchmarks found in the Phoronix Test Suite.
Google Sends Invites for September 15 India Event; Android One Launch Likely
Google has sent invitations for an event in India on September 15. While the invite itself says "More details closer to the date!", it is expected that the much-awaited Android One smartphones will make their debut at the event. Android One was announced back in June at Google I/O with India's Karbonn, Micromax, and Spice the confirmed launch partners, though more Indian companies have reportedly joined the list since then.
The jersey inside outwards, observe the situation embroidery section. Genuine jerseys are embroidered was "Z" shaped, embroidered on shirts, very smooth, in-house embroidery almost no sense of touch. Cheap nfl jerseys from china. The fakes are tight with the line directly to the number sewn on clothes.
Cheap nfl jerseys wholesale. Genuine Hawks player version jersey. Careful observation will find clothes with black "Z" shaped seams on clothes, that this line will be connected to the number and shirts, can make the wearer feel very comfortable, there will be uneven sense!
Finally, I want to make a comparison between true and false jersey, we want to want to buy a shirt to help a friend. Want to buy legal copies of NFL jerseys in the country is quite difficult. Domestic NFL licensed should be small. I discovered two years ago have sold Packers and bengals jerseys offset printing adults at a Reebok store in Xiamen. The price is very expensive, like the shelves soon after never seen. Now search for Taobao, filled with many counterfeit NFL jerseys player version. Cheap jerseys free shipping. Most of these jerseys rough work, by a number of embroidery, the workmanship is very sick.


text:
December is the time of year to reflect back on the past twelve months. Back in October, I had the chance to visit Pittsburgh for the first time, as well as attend my first Dublin Core metadata conference.
Need something?
What's hot?
The scientific names assigned to animals often have intriguing origins, which can be revealed more »
The Cooper-Hewitt Library is celebrating the release of Shedding Light on New York: Edward more »
The Smithsonian joins the Flickr Commons project on June 16! The Smithsonian Libraries provided more »
Recto: Abraham Woodside (born Philadelphia, PA, 1819; died Philadelphia, PA,1853) Verso: Carte-de-visite photographer: H. more »
Latest Tweets
Contact | Terms of Use | Permissions | Privacy | Smithsonian Libraries Website


text:
Police are on the hunt for the man who shot and killed two bail bondsman as they were detaining a fugitive Monday night.
Both victims were shot from behind, and the one person police do have in custody is not talking.
This is a first in Arizona. A bail bondsman has never been killed in the line of duty. Unfortunately, police don't know much about the shooter.
The bail bondsmen tracked their fugitive down to an apartment near 17th Avenue and Peoria. After a brief scuffle, they had 25-year-old Anthony Brian Giunta in handcuffs. Giunta's bond had been revoked.
They took him out to the street. Then moments later, the two bail bondsmen were shot from behind.
"For what happened they had to have been 100 percent caught off guard," said John Burns, president of the Arizona Bail Bondsmen Association.
John Burns knows the two victims.
"This was execution-style when you shoot someone from behind and you can't even face the person, that is a coward."
One of the victims is 37-year-old David Brickert. The other is 39-year-old Wesley Kampen. Both victims worked for Sanctuary Bail Bonds.
After the shooting, the killer took off and the fugitive who was handcuffed with his hands in front of him grabbed one of the victims' guns and ran off, only to later be caught by police. He isn't talking.
"He provided no information as to who this individual was who fired these round killing these two victims," said Phoenix Police Sgt. Steve Martos.
The victims were well trained bondsman. One even had a black belt in tae kwon do.
Burns says the entire industry is in shock and he has a warning for the killer.
"You have a nation full of bail bond agents. No matter what state you go to there will be an agent, that if he crosses your path, we will find you. The bail industry will not let this slide," said Burns.
The shooter is described as a white male, 25 to 20, wearing a white T-shirt and blue jeans. Anyone with information is asked to call Phoenix Police.
4739 NW 53rd Avenue, Sutie BGainesville, FL 32653
Phone: (352) 371-0051


text:
Please enter your email address to reset your password. An email will be sent with instructions to create a new password. If you do not receive an email, please check your spam folder.
Forgot password?
Don't have an account?
Are you a subscriber but don't have an online account?
Register for full online access.
Note to existing JLC Subscribers:
If you choose to use your Social Network, please ensure that the email address associated with your Social Network matches the email associated with your JLC Subscription.
Thank you for creating your JLC Online account! Your JLC subscription purchase has begun in a new window.
If you see this message, make sure your popup blockers are disabled and click here to relaunch the subscription window.
Congratulations! Please check your e-mail for confirmation to gain full access to JLC Online's free features.
Please create a nickname to post in the forums.
You won't go wrong with any of these tools, but vertical cutting capacity, portability, and innovative features
Laminated beams and PVC trim round off the corner on a new porch for a historic building
With a couple of specialty tools, you can offer this sleek look to your high-end customers.
Quacks in the siding aren't always a problem.
Find out which models will be used and review the installation specs before rough-in
Insulating knee walls; step flashings not the culprit; homeowner safety should come first
California back-pedals on nailer rules; study blames coastal development for increased storm damage; Texas court says insurers can't duck defect suits; more
Q: While we've never had problems with our tile-shower installations, new surface-applied waterproofing membranes and waterproof backerboards have me wondering if it's time to change our approach. We typically install cement backerboard over a poly membra
Q: A customer asked me about sealing a brand-new garage slab to keep oil drips from penetrating the concrete, in case she decides to have the slab finish-painted in the future. Is this necessary? What product should we use?
Q: A client wants to locate her dishwasher in a peninsula several cabinets away from the kitchen sink. Are there any limitations on the distance that the discharge hose can travel, or should it be plumbed separately?
Painting trim with latex; protecting blueprints from the elements
Our remodeling company has been using lead sheets — simple printed forms on which we record information about potential clients who call our office — for several years now.
I preach endlessly about job-site safety and the importance of regular safety inspections.
Shower pans; toilet seats
Plumbing; metal connectors
Max SN883 series framer; protected laser transport; roofing & siding; storage
If you don't know much about boatbuilding, start by welding rebar together to frame the gunwales and keel. Then form the hull with layers of chicken wire, and trowel a stiff 1/2-inch-thick mortar mix onto the mesh.
Copyright ©
2014 Hanley Wood Media, Inc. All rights reserved.


text:
- Articles
- Comics
- Interview
- News
- Serious News
- Videos
LMC's Top 5: Sonic The Hedgehog Moments – Part 4
Part 4 of 4: CapnCloudchaser reveals her Top 5 – incuding a bus, a cosplay and no sign of Doomsday Zone.
LMC's Top 5: Sonic The Hedgehog Moments – Part 3
Part 3 of the staff's personal rundowns sees Max Firestorm and Turbo talk flaming kitties and fangames.
Ten Years Of Sonic Wrecks – What Next?
2004 -2014…. and 2014-onward?
LMC's Top 5: Sonic The Hedgehog Moments – Part 2
LMC's reviewer T.A. Black looks over his own Top 5 best Sonic moments.
Comics – 113: Weren't We Telling A Story?
SW's 10 years celebration continues with a brand new comic going back and continuing the old storyline? Say it ain't so!
A Small Statement Regarding Sonic Wrecks Comic #99
A statement regarding drastic changes to "The Deepest Cut".
Comic #112: Evolution of The Machine
Take a trip to a dark future with the latest comic which looks at how "improvements" aren't always a good thing.
Random Reviews: Sonic Wrecks Comic #001
T.A. Black channels Linkara and reviews the very first SW comic for Sonic Wrecks' 9th birthday.
LMC Interview: How SEGA Helped Resurrect Typing Of The Dead OVERKILL
SEGA Europe's James Schall talks to LMC about a modern twist on a Dreamcast classic and the importance of Digital in SEGA's future.
Summer of Sonic – NiD.com Interview Kazuyuki Hoshino
TRiPPY & DiGi talk jesters and wizeman to the Sonic Team designer with the all the artistic answers.
Summer Of Sonic 2013 – An Interview With Takashi Iizuka
The head of Sonic Team talks to us about making Sonic Lost World and why the Wisps and Colours Powers are here to stay!
Jun Senoue & Johnny Gioeli SOS 2012 Interviews
JunSenoue.com interviews with Jun and Johnny at SoS 2012, filled with obscure questions!
First 4 Figures Reveal New 'Sword Kirby' Figurine
Standard and limited editions are coming right back at ya!
A Small Statement Regarding Sonic Wrecks Comic #99
A statement regarding drastic changes to "The Deepest Cut".
Capcom Shareholders Reject Anti-Takeover Plan
Shareholders say no to latest plans to prevent Capcom from being taken over – and cause fans to go into overdrive as to who would hypothetically buy the company
LAST CHANCE OFFER: Want to Make a Game? Now You Can!
Ever wanted to make your own game? Well now you can with National Game Development Month and a generous Humble Bundle deal, but gotta be quick!
Get Your 2014 Edition E3 Bingo Card Here
Back by popular demand for the third year running.
U.S. To Investigate Financial Activities Of Scrooge McDuck
Did Duck fiddle bills?
Outrage As BBC ONE Announces Video Games Only Schedule
Never mind the XBOX One's love of TV – the real scandal is TVs being used… for GAMING.
Paperboy Found Guilty Of Using Pixel Enhancing Drugs
Fallen icon declared cheater.
ALS Ice Bucket Challenge: Archie's Ian Flynn Takes Up Our Challenge!
The Sonic/MegaMan writer gets a dunking.
LMC Blind Bags: Minecraft Hangers
In three bags of mystery there awaits something… blocky.
LMC Unboxing: Loot Crate 'Adventure' Box
AAUK is back in the UK… and his post includes a Loot Crate. What's in it?
LMC Unboxing: Super Mario Easter Eggs
It's-a me! A tie-in! And for some reason we kept coming across egg-related Super Mario tie-ins.
Recent Reviews From Review
Recent Posts From All Categories
ALS Ice Bucket Challenge: Archie's Ian Flynn Takes Up Our Challenge!
The Sonic/MegaMan writer gets a dunking.
Let's Play Sonic Chronicles – Part 2: Life Was A Boar
Donnie's cat gets all sensitive about Big, Amy has buttons for eyes and AAUK creates "The RPG Grinding Song".
Let's Play Phoenix Wright: Ace Attorney – Case 1-4 (Pt 2)
Turnabout Goodbyes – Part 2


text:
I really want to go to a party at Tracey Overbeck and Ethan Stead's house. Aside from being smart, stylish and incredibly creative, this couple approaches entertaining with such an attitude of joie de vivre that the guests at their legendary dinner parties couldn't help but have the time of their lives. As you enter the gorgeous grounds that house their historic home, abundant with live oaks and pomegranate trees and surrounded by a tall stucco wall, it's easy to forget that you're minutes from downtown Austin. Since Tracey owns the eponymous acclaimed interior design firm, I knew that the Stead's home would be cool – but I wasn't prepared for the jaw-dropping details and individuality oozing from every corner. Since they spend half the year at their homes on Martha's Vineyard, the east coast and ocean play prominently into their entertaining style. On this particular day, Tracey and Ethan were prepping for an oyster party they were hosting for friends that evening. Take a step inside their home and click through the images shot by Chelsea Fullerton to see the perfect table setting, what they poured and how they prepped for an unforgettable oysters-in-Austin party.
Tracey's standard hostess outfit:
Tank top, jean shorts, diamonds, and of course, barefoot.
Dream dinner guests:
Michael Schumacher, Catherine Deneuve, Christopher Hitchens, David Bowie, and my late grandmother Katherine Pinckney Nau when she was in her 30′s.


text:
Request a Presentation
WISE Program
Graduate and Professional School Fair
First Name
Last Name
Address Abroad
Choose a term...
May 2013
Summer 2013
Both May and Summer
Fall 2013
Program Name
Choose a program name...
Political Science
Lycoming Affiliate
Non-Lycoming Affiliate
Passport Number
To Abroad
Return from Abroad
NOTE: The following must be taken to ONLY Career Services (3rd Floor Wertz) BEFORE APRIL 26:
Copy of Department of State Registration Confirmation
Copy of Required Health Insurance Coverage Abroad. This must include EMERGENCY EVACUATION AND REPATRIATION INSURANCE. If you do not plan to purchase special insurance for traveling abroad, you must verify that your US health insurance covers this.
Please enter the below text in the box provided and then click the submit button.
700 College Place
Williamsport, PA 17701
(570) 321-4000
Copyright © 2011 Lycoming College


text:
Remember Me
Dana Drouin is justifiably proud of her photo albums. In one a trip to the shore is carefully documented -- snapshots show a pair of cheerful toddlers splashing in the surf, a serious older boy digging in the sand, a gang of adults hanging around a beach house, grinning. Another catalogs a trip to Disney World, while a third testifies to the hectic but happy life Dana shares with her husband, Shawn, their three children -- Ryan, 12, and twins Sydni and Aidan, 5 -- and their dog, J.J. "This is from last year," Dana says, pointing to a shot of Shawn and Sydni at a tearoom near their home in Blackwood, New Jersey. In the photo Shawn, who's 6-foot-4 and built like a linebacker, sits delicately on a wrought-iron chair. Tenderly, he leans toward a beaming Sydni, dressed in her Christmas best, offering her a tiny tea cake. Dana, who took the photograph, remembers the occasion with bittersweet fondness; for the Drouins, high tea with their daughter at Christmas is more than just a holiday treat. Three and a half years ago Dana was diagnosed with stage IV terminal cancer; the tea party is a special ritual she'll share for as long as she can. After she's gone, Shawn and Sydni will celebrate alone.
In the summer of 2003 things were finally coming together for the Drouins. They'd spent years trying to have another baby, and finally -- to 6-year-old Ryan's delight -- conceived the twins through in vitro fertilization. They'd survived the serious complications that sent Dana to the hospital several times during her pregnancy, and she'd weathered two whole trimesters of bed rest. They'd even managed to move to a bigger house after the twins were born and had taken out a second mortgage to cover their anticipated extra expenses (Dana planned to return to her job as a certified medical assistant when the twins turned 2). In her new home, Dana felt she could finally relax. "Life had certainly thrown us a few curveballs, but now I felt that all our dreams were within reach," she remembers. And then, when the babies were 9 months old, her back began to hurt.
At three separate visits over the course of an entire year, her doctor told her not to worry -- chances were she'd pulled a muscle lifting the twins. So the pain came and went, until one day it became so intense that Dana could barely breathe and had to race to the doctor again. Thinking that she had kidney stones, her doctor ordered a CT scan and sent her to a urologist. The urologist told Dana she didn't have kidney stones, but that she needed to go back to her primary care physician right away because she had lesions on her kidney, spine, and liver. "Right then I knew I had cancer," Dana says.
The oncologist she was later sent to was baffled. Dana's blood work came back without any of the usual markers for cancer, but an MRI showed a massive tumor on one of Dana's vertebrae, which a biopsy confirmed to be malignant. "Then I had a PET scan, which lit up like a Christmas tree," Dana says. The scan showed more tumors on her spine, on her right shoulder, throughout her liver, and in her kidneys. She was 31 years old.


text:
New day. New Daily Deals. Get them before they are gone!
New Customer ? Start Here.
Can't find what you're looking for? Shop all ink and toner cartridges by brand
New day. New Daily Deals. Get them before they are gone!
Item: 762047 Model: 545-15-154
|Hammers & Mallet Face Size
|1 - 2 in Diameter
|Hammers & Mallet Type
|Hammer & Mallet Accessories
|Hammers & Mallet Overall Length
This Web site is intended for use by US residents only. See International Sites. See our delivery policy for full details. Copyright 1998-2014, Staples, Inc., All Rights Reserved.