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Lambeth Council pledges to help crack prostate cancer22 Mar 2013Jim Dickson backs initiative to tackle the disease in England's most affected Borough. Read more
Vast variation in quality of prostate cancer care revealed across England13 Mar 2013New analysis unveiled today has uncovered vast variations in the care that men with prostate cancer receive across the whole of England. They reveal that many men in the West Midlands and Essex are receiving poor prostate cancer care and support whilst the majority of men living in Greater Manchester are getting what they need.Read more
Abiraterone letter to editor15 Mar 2012 Dear Sir, March is Prostate Cancer Awareness Month. In a few weeks time NICE will meet to discuss the responses to their draft decision not to recommend abiraterone, a life extending drug for men with incurable prostate cancer. Read more
Charity warns "short sighted" NICE risks compromising care of men with prostate cancer14 Mar 2012 Men with prostate cancer are at risk of being left behind those with other common cancers thanks to a spate of recent moves by the National Institute for Health and Clinical Excellence (NICE), The Prostate Cancer Charity has warned. Read more
The Prostate Cancer Charity criticises SMC abiraterone decision12 Mar 2012 We have criticised the Scottish Medicines Consortium's (SMC) decision not to recommend abiraterone for use on the NHS in Scotland. It follows the National Institute for Health and Clinical Excellence's (NICE) draft decision to deny the drug for men on the NHS in England and Wales. Read more
The Prostate Cancer Charity comments on a new study examining prostate cancer screening31 Mar 2011 The Prostate Cancer Charity comments on new research, published in British Medical Journal, which examines the impact of prostate cancer screening on mortality. Read more


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Show your Wildcat spirit with this University Of Kentucky boyshort panty. Features your school colors on signature stretch floral lace and your school logo in crystal brilliants. Made of nylon.
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This page contains Harry Potter: Quidditch World Cup Hints for Xbox called "Faster brooms" and has been posted or updated on Oct 16, 2006 by ubeatyggo. Let us know what you think of this Hint by thumbs voting.
- Developer: Electronic Arts
- Publisher: Electronic Arts
- Genre: Sports Alternative Other
- Release Date: Oct 28, 2003
- ESRB: Everyone
Faster brooms
If you want a really fast broom and play dirty team specials, choose Slytherin.
They have Nimbus 2001 brooms and their special gets you 20 points. Japan has
Firebolts, but does not have a strong team.
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Feel like going commando but aren't quite brave enough to bare it all? Gregg Homme's Commando has you covered (just barely) with this super lightweight, airy trunk. You'll feel as if you're wearing nothing at all but still be sporting a stylish pair of underwear. Features a black waistband with Gregg Homme printed around with either a red or blue body.
says Alan
Wednesday, August 08, 2012 - True To Size
This is made of light and very stretchy sheer material. It conforms well to the male anatomy without being squashed and has no center seam. Works well in warm weather.Was this helpful? Sign in to vote.
Size | Inches | Centimeters |
Small (S) | 29-31 | 74-79 |
Medium (M) | 32-34 | 81-86 |
Large (L) | 34-36 | 86-91 |
Extra Large (XL) | 36-38 | 91-97 |
Here are some of the most popular products (by sales volume) for this style:


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UCLA unveils "Time to Come Home" video after Tony Parker commitment
Apr 25, 2012, 5:59 PM EST
With the renovated Pauley Pavilion set to open in the fall and a Top 3 recruiting class in place, UCLA is reemerging. The marketing department is making a push, too.
In a video released Tuesday, the Bruins play up their historic past, set to some epic music.
How did it come out? You tell us. Check it out below.
Daniel Martin is a writer and editor at JohnnyJungle.com, covering St. John's. You can find him on Twitter:@DanielJMartin_
No. 21 New Mexico falls victim to unexpected defensive wrinkle
Mar 9, 2014, 12:56 AM EST
New Mexico led by 16 with 12:05 remaining. And then their inability to adjust to San Diego State's 1-3-1 zone cost them the Mountain West title.
No. 10 SDSU uses 26-4 run to beat No. 21 New Mexico, win MWC title
Mar 9, 2014, 12:50 AM EST
No. 10 San Diego State overcomes a 16-point second half deficit as they knock off No. 21 New Mexico and bring home a Mountain West title. But should that change how we view the Aztecs?
Rebounding to blame for No. 14 North Carolina's loss at No. 4 Duke
Mar 9, 2014, 12:09 AM EST
North Carolina shot nearly 60%, but getting whipped on the boards cost them dearly at Cameron.
Bubble Banter: Oregon, Tennessee headline Saturday's early winners
Mar 8, 2014, 11:16 PM EST
Get caught up on all of Saturday's bubble action
Creighton's Doug McDermott scores his 3,000th point (VIDEO)
Mar 8, 2014, 9:38 PM EST
Creighton's senior forward scored his 3,000th career point on Saturday night. Only seven other players have reached that total.
Perimeter shooting, quiet days from role players cost No. 3 Arizona
Mar 8, 2014, 7:49 PM EST
No. 3 Arizona hasn't been the best perimeter shooting team, but they've been able to make up for that on most days. That wasn't the case against Oregon, and the struggles of Rondae Hollis-Jefferson and Gabe York didn't help either.
Texas A&M celebrates life of deceased high school signee Tobi Oyedeji on Senior Day
Mar 8, 2014, 7:09 PM EST
Texas A&M celebrated Senior Day on Saturday and it included honoring former high school signee Tobi Oyedeji, who was tragically killed in a car accident in 2010 and never played for the school.
Oregon's big win over No. 3 Arizona caps impressive regular season turnaround
Mar 8, 2014, 6:45 PM EST
Oregon wins its seventh straight, and the Ducks likely punched their ticket to the NCAA tournament with the win over No. 3 Arizona.
No. 19 UConn's offense continues to struggle in embarrassing road loss at No. 11 Louisville
Mar 8, 2014, 6:15 PM EST
No. 19 UConn has struggled to crack 70 points during a recent six-game stretch and it could lead to an early exit for the Huskies in the postseason.
Coin flip lands Cincinnati top seed in American Athletic Conference tournament (VIDEO)
Mar 8, 2014, 6:01 PM EST
The top seed in next week's American Athletic Conference was determined by an old school method Saturday afternoon.
No. 2 Wichita State improves to 33-0, moves to Missouri Valley title game
Mar 8, 2014, 6:00 PM EST
Cleanthony Early scored 20 points to lead No. 2 Wichita State to a 67-42 rout of Missouri State in the semifinals of the Missouri Valley tournament. The Shockers improve to 33-0 on the season with the victory, one game away from heading into Selection Sunday with an unblemished record. They will play the winner of…
As Montrezl Harrell continues to emerge, is No. 11 Louisville a threat to repeat?
Mar 8, 2014, 5:06 PM EST
As Louisville sophomore forward Montrezl Harrell puts up huge numbers against great competition in the last few weeks are the Cardinals a threat to make a run at back-to-back national titles?
Naz Long forces OT in No. 16 Iowa State's come-from-behind win over Oklahoma State (VIDEO)
Mar 8, 2014, 4:14 PM EST
At this point, there should be no questioning whether or not Oklahoma State is back to being the team that they were back in December. If it wasn't for a missed free throw from Phil Forte, an 88.9% free throw shooter, and a buzzer-beating three from Naz Long at the end of regulation, the Cowboys…
No. 8 Kansas loses, but The Andrew Wiggins Show is what's important
Mar 8, 2014, 3:34 PM EST
No. 8 Kansas fell on the road to West Virginia on Saturday, but the Jayhawks will take the loss knowing that the superstar version of Andrew Wiggins might have finally come to life.
No. 1 Florida's undefeated SEC season not just a by-product of their league
Mar 8, 2014, 2:41 PM EST
No. 1 Florida spent Saturday showcasing just what makes them so dangerous heading into the NCAA tournament.
No. 20 Memphis overcomes sluggish start in win over No. 18 SMU
Mar 8, 2014, 2:36 PM EST
No. 20 Memphis is known for the play of its senior guards, but does their improving frontcourt give them a chance at a postseason run?


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Sorry if this is terribly elementary. I thought I was setting the margin for the document but it is only for the page. How can I set margins for the whole document? I couldn't find it in the Document Setup dialogue box.
Thank you!
The margins you set initially in document setup are used for the None and A master pages. After that, waht happens depends on what is selected. Selecting a page or pages in the pages panel will apply the new margins to that page or pages, selecting a master page will apply them to all pages based on the master.
To add to Peter's post;
If you have changed the margins on a live page -
You can go to the master page(s) and define the margins which will apply to the whole document, except any pages that have been set locally.
Re-apply the Master Page to the document page(s) and the margins will revert/reflect the Master Margins
Set them on the master page. if they're the same as the ones you did on an individual page you don't need to update those unless you change your mind again. As Daniel said, reapplying the master page will reset the margins to the ones onthe master, but it may have some other side effects, like making new master text frames, or leaving some existing frames at the old size. You can minimize the problems with frames not adjusting to the new margins by enabling Layout Adjustment (prior to CS6) using Layout > Layout Adjustment BEFORE you make any changes.


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"People have asked me about that. If motivational speaking was the way to coach a football team, there would be a whole lot of motivational speaking football coaches,'' Holgorsen said. "Their presence is appreciated, but if you think a few of their words are going to change the outlook of a football team, you're off your rocker.
"We appreciated their support and those guys are important to the program. But motivational speaking is not the answer when it comes to a well-prepared football team."
West Virginia's defense is among the worst in the nation and Iowa State relies on its defense to win football games, right? That's the prevailing perception.
At least some of the statistics, though, contradict that view.
For instance, during WVU's awful five-game losing streak the Mountaineers have surrendered an average of 533 yards per game. But over its last five games, two of them wins, the Cyclones have actually surrendered more yards, an average of 561.8 per game.
West Virginia still trails Iowa State in most defensive categories in the NCAA rankings, including total defense (ISU is 93rd, WVU 117th). But the Cyclones' pass defense isn't much better (No. 110) and WVU's run defense has an edge (29th vs. 59th).
But those numbers come with a caveat. Despite the yards, Iowa State is not giving up points at nearly the rate of West Virginia. The Cyclones are giving up just 22.6 per game and are the only league school that hasn't given up more than 35 points in a game. WVU has given up 34 or more eight times in 10 games and surrendered 45 or more six times.
Reach Dave Hickman at 304-348-1734 or dphickm...@aol.com or follow him at twitter.com/dphickman1


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Plus You Look Cool: Supine Reading Glasses
I'm pretty sure these $50 Supine Reading Glasses have been around for awhile because I remember using them to look up girls' skirts while breakdancing, but here they are, just in case you didn't know they existed. Think of them as periscopes. Really dorky-ass periscopes you wear to bed. Booboobooboop -- booboobooboop.
Reading while lying down tends to involve propping your head up with uncomfortable pillow arrangements, often with your neck bent in angles that will result in a moderate amount of pain the following day. These reading glasses bend light 90º to save you that trouble.
Not to brag or anything, but did you know one time I strong-armed light and bent it 365º? Because I did. Sure it created a worm-hole and swallowed my neighbor's cat, but NO, FOR THE LAST TIME I SWEAR TO GOD THAT'S WHAT HAPPENED I DID NOT BACK OVER HIM (what kind of animal falls asleep behind a f***ing tire?!).
Product Site
Supine Reading Glasses Reduce Need to Raise Head [uberreview]
So allegedly there's a new eye surgery that allows patients to achieve 'HD vision'. Weird, I thought all vision was high definition. But what the hell do I know? I'm no ophthalmologist, I'm just a guy that spiced up an ordinarily boring eyeball graphic with LASER VISION!!! ... / Continue →
Gunnar glasses ($100 - $189!!!!!!!!!!) come in cleverly named styles like Bit Surfer, Wi-Five and El Doucherino, and are supposed to prevent the eye fatigue caused by blogging eight hours a day. That's right ladies and gentlemen....prepare to experience "Enhanced Computer Visi... / Continue →
Because George Lucas believes that absolutely everything should be available with a Star Wars theme, here's a line of glasses from Japanese eyewear manufacturer Aigen. They're available in Luke Skywalker, R2-D2, Stormtrooper, Boba Fett and Darth Vader designs and cost $500 wit... / Continue →


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CD/LP/Track Review
James Blood Ulmer: No Escape From The Blues (2003)
2003 was declared the year of the blues by Congress and what has flooded the market has been a series of reissues and recompiling of older material that give the appearance that blues has gone as far as it can go as a progressive music – and perhaps the best recordings made were accomplished years ago. Ulmer has taken a full grip on the historical blues flag, but rather then emulate and copy the greats of yesteryear he has brought his keen and fresh take on the music, proving that the blues can be both traditional and modern – perhaps it is his jazz background or just a wonderfully fertile mind, but Ulmer succeeds where others have failed.
On these Electric Lady Sessions, Ulmer is produced adeptly by guitar legend Vernon Reid, whose career, too, has been one of progression and experimentation but always with a general audience in mind. They use the form of some of the staples of the blues song-bag, but open it up to tap dancing, trumpet solos by Olu Dara (both on Jimmy Reed's "Bright Lights, Big City"). Ulmer channels the spirit of Muddy Waters (for his harmonica style and song structure) and Jimi Hendrix (for his guitar sound and approach, and in whose studio this album was recorded) for Waters' "No Escape from the Blues." Ulmer takes his own song, "Are You Glad to Be in America," and records it as if he were an artist today recording for a pre-WWII blues label like Paramount or OkeH.
This recording already has my vote for blues album of the year. It is a perfect synergy of old and new, proving the point that the blues is not an old musty genre. The blues can and does progress and sound as current and timely as any other style of music.
Dare I say masterpiece or landmark recording?
Track Listing: 1. Goin' to New York
2. The Hustle is On
3. Who's Been Talkin'
4. Ghetto Child
5. Are You Glad to Be In America
6. You Know, I Know
7. Come On
8. Bright Light, Big City
9. No Escape from the Blues
10. Satisfy
11. Trouble in Mind
12. The Blues Had a Baby and Called It Rock and Roll
Personnel: James Blood Ulmer - vox, guitar; Vernon Reid - Guitar, Sitar and Banjo; Leon Gruenbaum - Keyboards; Charlie Burnham - Fiddle and Bass; Aubrey Dayle - Drums; Queen Esther - Vox; David Barnes - Harmonica; Olu Dara - Trumpet; John Kruth - Tamboura; Maya Smullyan Jenkins - Tap Dancing.
Record Label: Hyena Records
Style: Blues


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"This recipe is EXTRAORDINARY! I have been making this for the past few years and it's the only stuffing recipe I use. Simply perfect!" – Coastal Roots
Recipe of the Day
Baked Chicken with Apple Stuffing
Breaded chicken breasts are baked with a simple apple and bread stuffing for an easy one dish meal. Get cozy with this holiday-inspired meal anytime of year! — SCOTTBMA72
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Sausage stuffing gets the sweet-tart treatment with green apples and cranberries.
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Everything you need to party like a leprechaun.
New for spring! Good-for-you food you'll love to eat.
Delicious recipes, party ideas, and helpful cooking tips! Get a year of Allrecipes magazine for just $9.99!
See how to make the perfect Thanksgiving stuffing for seafood lovers.
This stuffing blends sausage, turkey liver, fruits, and veggies.


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1/2 cup chopped Vidalia onion 1/2 cup chopped red pepper 1 jalapeño pepper, finely chopped 3 T. parsley flakes 1 T. plus 1/4 cup olive oil 8 peaches, peeled, pitted and chopped 1 cup water 1/2 cup sugar 1/4 cup apple cider vinegar 1 tsp. salt 1/2 tsp. chili powder 1/4 tsp. black pepper 1/4 tsp. garlic powder 3 lbs. trout fillets Onion powder, to taste Black pepper, to taste
In large skillet, sauté onion, pepper, jalapeño and parsley in 1 tablespoon olive oil over medium heat for 5 minutes or until tender. Add peaches, water, sugar, apple cider vinegar, salt, chili powder, black pepper and garlic powder to skillet; mix well. Cook over medium heat for 30 minutes, stirring frequently. Refrigerate salsa at least 2 hours before serving. Grill trout fillets in 1/4 cup olive oil. Add onion powder and black pepper. Serve peach salsa over trout.
Peter J. Carmon Cody, Wyoming
From— The Water's Bounty: Member Fish Recipes
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Sixty-seven lakes and reservoirs, in 19 states, hold specially tagged fish that could turn into cash or prizes for lucky anglers! Read on!
Poor Man's Lobster
Place vegetable oil in skillet. Fry fish until half done.
Bluegill Sauté


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Pass Labs Goes Outsized
Pass Labs showed off two nice beer fridges, er, amplifiers at this year's CES. Lacking any model numbers or nomenclature, the top monoblock amp (the top two units) is a single-ended 200W amp ($45,000/pair) and the bottom monoblock is a single-ended 300W amp ($75,000/pair). Nelson Pass is shooting to use no feedback on these circuits and maintain the Super Symmetry design made popular in far less extreme applications. The new part for these amps is a brand new silicon-carbide FET. These FETs were initially designed for the militaryway to turn swords into plow shares and space heaters, Nelson!and Pass Labs intends on exploring their use in future products.
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Apple's colour touchscreen is triggering both fear and excitement among print publishers. But how will consumers embrace the newformats? »
Like iPhone apps, Apple's new tablet promises to make web content streamlined, friendlier and more accessible »
"Better than the laptop and better than the smart phone" -- Apple claims to have created a whole new genre of device in one of the most anticipated products for years »
Content creators, not gadget freaks, will be the biggest target of Apple's press conference later today »
After a downbeat 2009, optimism returned to the tradeshow floor in Las Vegas, led by advances in 3D TV and hopes that tablet PCs will bring back profits »
As netbooks, e-book readers and now tablets proliferate, a certain amount of confusion is developing over terminology »
As Sony set out its plans for the year ahead, something tablet-like flashed onto the screen. The tech press stirred themselves, but it was all in vain »
As the world awaits Apple's mythical tablet, Freescale Semiconductor has got in there early with a low-cost tablet concept design »
Video was 'the one that got away' from Apple, but recent moves reveal the company is taking a second stab at the category, and that streaming video will play a big role »
A Russian professor has developed a cure for sobriety, but possible side effects include headaches, disorientation and bad dancing »
A hands-on with the beautiful but flawed internet tablet that has emerged from the ashes of TechCrunch's failed Crunchpad project »
The company's dogged attempt to build the Crunchpad - a cheap, Linux-based tablet device - has controversially imploded, just two weeks from its alleged release date »
Jobs is considering his legacy, and he wants it to include saving the media, pulling it back from the brink at its darkest moment »
An Australian paper claims to have received specs for Apple's tablet, as the company reportedly meets with publishers to discuss the future of digital media »
While the world awaits Apple's fabled touchscreen tablet, Microsoft has unveiled a rather svelte one of its own, featuring dual seven-inch multi-touch screens »
Insiders claim a tablet and a touchscreen phone based on the company's Surface technology will be unveiled in early 2010 »
Toshiba has delivered one of the few surprises of this year's IFA tech show with the Journe Touch, a tablet that could steal Apple's thunder »
Apple will be launching something in September, but no one knows what. That hasn't stopped us all from indulging in a spot of speculation »
An industry pundit who says he has spoken to component makers has predicted the device's size, price and features – and a 2010 launch date »
With an Apple tablet all but a certainty, news emerges that Intel and Dell are working on a similar device. Has the tablet's time come at last? »
In what is best described as a flash memory willy-waving demo, Kingston has unveiled a 1TB USB thumbdrive -- that's over a thousand gigabytes on your keyring »
With its vast tin reserves, the indonesian island of bangka is experiencing a mining rush that has led to environmental damage and human suffering. The cause? global demand for the solder used to make our smartphones and tablets »
South Park: The Stick of Truth is a game that fans of both role-playing games and South Park will love »


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Fish Magnetic Wine Bottle Foil Cutter
Fish Magnetic Wine Bottle Foil Cutter
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The Fish Magnetic Wine Bottle Foil Cutter will stay put on the refrigerator until you need to remove that stubborn foil from the top of a wine bottle. The Fish Magnetic Wine Bottle Foil Cutter has a metal foil cutter built in that you simply place atop the wine bottle, twist the foil cutter and the foil is removed. Besides being functional and adorable, the Fish Magnetic Wine Bottle Foil Cutter doubles as a refrigerator magnet when not on duty as a foil cutter.
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The Acrylic Wine Glasses - Fish Out of Water are a set of two unbreakable plastic wine glasses perfect for pool or beach. Fish Out of Water Acrylic Wine Glasses have one wine glass right side up and one glass upside down to see the recipe on the bottom
Fish Wine Bottle Holder
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The Fish Wine Bottle Holder is the perfect holder for fine wines in a lake cabin or beach home. The Fish Wine Bottle Holder cleverly holds a favorite bottle of wine and makes a charming nautical wine bottle caddy. NOTE: The wine bottle is not included.
Fish Wine Glass Woozie by Carol Eldridge
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The Fish Wine Glass Woozie by Carol Eldridge is a wine glass sleeve made of neoprene. The Fish Wine Glass Woozie is a whimsical, impressionistic Woozie wine glass cover perfect for summer days spent relaxing at the beach or lake with a glass of wine.
Flip Flop Magnetic Foil Cutter
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The Flip Flop Magnetic Wine Bottle Foil Cutter can be used as a refrigerator magnet until you need to cut the foil from a wine bottle. Put the Flip Flop Magnetic Wine Bottle Foil Cutter on the wine bottle, twist the foil cutter and the foil is removed!
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Fish Magnetic Wine Bottle Foil Cutter - cute and inexpensive gift
Christi Fewkes
(Morgan Hill, CA)
They have such a variety of cute and inexpensive foil cutters. My friend is a wine and fish lover, so I got this as a gift and it was a huge hit! It's great when you can find a small gift idea for a reasonable price, especially for those who have everything ;)
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Argentina - Salta & the Andean Northwest (Chapter)
This is the Salta & the Andean Northwest chapter from Lonely Planet's Argentina guidebook.
Argentina's northwest sits lofty, dry and tough beneath the mighty Andes. There's a definite Andean feel with traditional handicrafts, Quechua-speaking pockets, coca leaves, llamas, indigenous heritage and Inca ruins. The region's cities were also Argentina's first colonial settlements.
- wonder at nature's palette in the Quebrada de Humahuaca
- maps
- observe weavers at work in the memorable Valles Calchaquíes
- transportation
- cleanse your lungs in the crisp mountain air of Tafí del Valle
- restaurants & accommodations
Coverage includes: Jujuy, Quebrada De Humahuaca, Purmamarca, Tilcara & around, Uquía, Humahuaca, Iruya, La Quiaca, Yavi, Salta, Tren a las Nubes, San Antonio de los Cobres, Salinas Grandes, Valles Calchaquíes, Cachi, Seclantás, Molinos, Angastaco, San Carlos, Cafayate, Quebrada de Cafayate, Tucumán, Tafí del Valle & around, Santa María, Amaicha del Valle, Quilmes, Santiago del Estero, Catamarca, Belén & around, La Rioja, Chilecito and Parque Nacional Talampaya.
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Good-bye, Google Reader. Hello, Shopping Express!
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A new website for finding allergy friendly recipes and products is now beta testing.
Plus McDonald's Fruit and Maple oatmeal doesn't actually contain any maple syrup, and concerns over dioxin-tainted animal feed spreads to France and Denmark, all in our morning news roundup.
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The program makes a record of what you drink that you can recall with any web browser.
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Parental Origin and Timing of De Novo Robertsonian Translocation Formation The American Journal of Human Genetics, Volume 71, Issue 6, 1 December 2002, Pages 1456-1462 Ruma Bandyopadhyay, Anita Heller, Cami Knox-DuBois, Christopher McCaskill, Sue Ann Berend, Scott L. Page and Lisa G. Shaffer Abstract Robertsonian translocations (ROBs) are the most common chromosomal rearrangements in humans. ROBs are whole-arm rearrangements between the acrocentric chromosomes 13–15, 21, and 22. ROBs can be classified into two groups depending on their frequency of occurrence, common (rob(13q14q) and rob(14q21q)), and rare (all remaining possible nonhomologous combinations). Herein, we have studied 29 case subjects of common and rare de novo ROBs to determine their parental origins and timing of formation. We compared these case subjects to 35 published case subjects of common ROBs and found that most common ROBs apparently have the same breakpoints and arise mainly during oogenesis (50/54). These probably form through a common mechanism and have been termed "class 1." Collectively, rare ROBs also occur mostly during oogenesis (7/10) but probably arise through a more "random" mechanism or a variety of mechanisms and have been termed "class 2." Thus, we demonstrate that although both classes of ROBs occur predominantly during meiosis, the common, class 1 ROBs occur primarily during oogenesis and likely form through a mechanism distinct from that forming class 2 ROBs. Abstract | Full Text | PDF (243 kb) |
Uniparental Disomies in Unselected Populations The American Journal of Human Genetics, Volume 63, Issue 4, 1 October 1998, Pages 962-966 Eric Engel Full Text | PDF (58 kb) |
Obligate Short-Arm Exchange in De Novo Robertsonian Translocation Formation Influences Placement of Crossovers in Chromosome 21 Nondisjunction The American Journal of Human Genetics, Volume 72, Issue 2, 1 February 2003, Pages 488-495 Sue Ann Berend, Scott L. Page, William Atkinson, Christopher McCaskill, Neil E. Lamb, Stephanie L. Sherman and Lisa G. Shaffer Abstract Robertsonian translocations (ROBs) involving chromosome 21 are found in ∼5% of patients with Down syndrome (DS). The most common nonhomologous ROB in DS is rob(14q21q). Aberrant recombination is associated with nondisjunction (NDJ) leading to trisomy 21. Haplotype analysis of 23 patients with DS and de novo rob(14q21q) showed that all translocations and all nondisjoined chromosomes 21 were maternally derived. Meiosis II NDJ occurred in 21 of 23 families. For these, a ROB DS chromosome 21 genetic map was constructed and compared to a normal female map and a published trisomy 21 map derived from meiosis II NDJ. The location of exchanges differed significantly from both maps, with a significant shift to a more distal interval in the ROB DS map. The shift may perturb segregation, leading to the meiosis II NDJ in this study, and is further evidence for crossover interference. More importantly, because the event in the short arms that forms the de novo ROB influences the placement of chiasmata in the long arm, it is most likely that the translocation formation occurs through a recombination pathway in meiosis. Additionally, we have demonstrated that events that occur in meiosis I can influence events, such as chromatid segregation in meiosis II, many decades later. Abstract | Full Text | PDF (481 kb) |
Copyright © 2000 The American Society of Human Genetics All rights reserved.
The American Journal of Human Genetics, Volume 66, Issue 6, 1787-1793, 1 June 2000
Sue Ann Berend1, Juli Horwitz2, Christopher McCaskill1 and Lisa G. Shaffer1, ,
1 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston
2 Genzyme Genetics, Santa Fe
Rearrangements of the acrocentric chromosomes (Robertsonian translocations and isochromosomes) are associated with an increased risk of aneuploidy. Given this, and the large number of reported cases of uniparental disomy (UPD) associated with an acrocentric rearrangement, carriers are presumed to be at risk for UPD. However, an accurate risk estimate for UPD associated with these rearrangements is lacking. A total of 174 prenatally identified acrocentric rearrangements, including both Robertsonian translocations and isochromosomes, were studied prospectively to identify UPD for the chromosomes involved in the rearrangements. The overall goal of the study was to provide an estimate of the risk of UPD associated with nonhomologous Robertsonian translocations and homologous acrocentric rearrangements. Of the 168 nonhomologous Robertsonian translocations studied, one showed UPD for chromosome 13, providing a risk estimate of 0.6%. Four of the six homologous acrocentric rearrangements showed UPD, providing a risk estimate of 66%. These cases have also allowed delineation of the mechanisms involved in producing UPD unique to Robertsonian translocations. Given the relatively high risk for UPD in prenatally identified Robertsonian translocations and isochromosomes, UPD testing should be considered, especially for cases involving the acrocentric chromosomes 14 and 15, in which UPD is associated with adverse clinical outcomes.