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I was wondering if apples could really be grown in the tropics? Now by tropics I don´t mean Florida, but the real tropics like Central America, or Southern India or even Thailand, and not the tropical highlands either, pretty much at sea level up to maybe 300 meters/1000 feet max. A typical tropical climate consists of a warm dry season and a warm wet season, with temperatures typically above 68F/20C all the time, so there is absolutely zero chill.
I know Dorsett Golden comes out of the Bahamas, but the Bahamas do get occasional cold blasts. Both Anna and Dorsett Golden are known to set multiple crops a year in California, and the quality of these apples is exceptional. But I still have a hard time wrapping my head around the idea that an apple would do well in a truly tropical climate. I came across this blog from a woman in Jamaica that was actually trying to grow apples from Gala seeds: See The Coconut Chronicles. Anyone have any thoughts on this? I know applenut has blown out of the window most conventional ideas about apples needing high chill. |
Follow-Up Postings:
Axel: Yes. Dormancy is induced by training the branches horizontal and stripping the leaves by hand to coincide with the onset of the dry season, at which time any irrigation is shut off. This causes the trees to blossom in about six to eight weeks. Chemicals like Dormex have been tried, but the hazard and expense they pose makes them unusable in most locations. Apples are grown commercially in Honduras, Nicaragua, the Philippines, Indonesia, and Egypt. Uganda did a large study in the Kabale region in 2005, and published a 70-page paper on it that you can Google. In Asia the main problem the indigenous farmers face is cheap imports from China crowding out their local market, making it impractical to grow apples even if the climate allows it. The main issues facing the tropical farmer has been the short shelf life of the low-chill Anna and Dorsett Golden, plus all the disease that a tropic climate can breed on apples. They've tried growing all the commercially popular apples like Jonathan and Red Delicious, which is silly as the quality would be terrible, as they have found out. This may seem like a hair-brained effort to try, but the rewards can be huge. Africans dream of eating an apple someday, as they represent prosperity to them. In an East African market apples from South Africa sell for a dollar a piece, well out of the range of most people. But a farmer that harvests three hundred apples off his tree can send his kid to University that year. Uganda had focused its efforts on growing apples commercially instead of just subsistance farming, as this is the key to lifting people out of poverty. I'm convinced the key to success will be in finding the right varieties, which most likely will not be what we would consider a standard low-chill apple. I'm focusing on the disease-resistant and long-keeping varieties like Williams' Pride and Enterprise from the PRI breeding program that don't need cool nights to color up and have shown to be able to tolerate a good deal of heat. Lots of testing will have to be done. Which presents the problem; first of all getting apples through customs (even with an import permit this can be tricky due to corrupt officials), transported to farmers, educating the farmers on apple culture, and getting starving people to give up some of their worn-out farmland to test a crop that has never been tried there before and not cut them down for firewood before they've bore the first crop. It will take a miracle of God to make this happen, which is what I'm counting on as I'm shipping 200 trees to Rwanda the end of this month to an island in Lake Kivu. Pray for the mayor of the island, as we are asking him for government land to plant them on. Last I heard last week he had Typhoid fever and was very ill (the things we take for granted in this country). Like I said, it will take a miracle of God- which could happen. Applenut |
why not try to breed low chill apples with the PRI selections? It would seem to me that some breeding is in order. I have heard from southern floridians that Anna produces only small apples down in Miami. Is that true? |
I live in Grenada a small Island in the Caribbean and there are a few cases where people have planted apples and successfully gotten it to fruit, One important fact that people over look is that apples need another plant because they cross pollinate. I too have grapes and a small apple plant about 4 inches. |
Your plant looks good, and will grow rapidly to about two meters this season. I get a lot of emails from people in the tropics who have planted apple seeds and are surprised when they sprout and grow vigorously. However, as a seedling tree it will take a while to fruit, and most likely produce poor quality apples. You can graft a good variety onto it, but usually bud or scionwood is hard to find in these tropic countries. |
- Posted by SelfMadeBum none (My Page) on Thu, Jan 24, 13 at 5:46
I live in Barbados and this is *fascinating* to me. Please keep us updated on your progress, Brato. |
- Posted by Fascist_Nation USDA 9b, Sunset 13, (My Page) on Thu, Jan 24, 13 at 14:09
And a freebie: "growing apples in the tropics" [pdf] http://www.kuffelcreek.com/GrowingApples/GrowingApplesTropics.pdf |
Here is a link that might be useful: African Apple Nursery
- Posted by Fascist_Nation USDA 9b, Sunset 13, (My Page) on Thu, Jan 24, 13 at 15:17
And a freebie: "growing apples in the tropics" [pdf] http://www.kuffelcreek.com/GrowingApples/GrowingApplesTropics.pdf |
Here is a link that might be useful: African Apple Nursery
I have about 10 trees total now, thanks Fascist_Nation I appreciate the information I'll do some reading and experimenting when it comes to care and maintenance. SelfMadeBum I'll update with pics soon |
- Posted by melikeeatplants 9b (San Jose) (My Page) on Thu, Feb 14, 13 at 16:16
This is a good bump, what happened to those 200 trees sent to Rwanda? |
They are bearing, but have a typical upright, unproductive growth habit of an apple tree in the tropics that was not trained during growth; long, vertical branches growing tight against the trunk. In order to foster bearing the young trees must be trained by bud notching and pulling the young branches down below horizontal to form fruit buds. Our apple tree nursery in Uganda includes post-harvest training for two years with the purchase of a tree in order to make sure that they are trained correctly for productive growth. |
As promised here are the pics and up dates |
You can contrast the Rwanda trees with these in Zambia that have had some rudimentary branch spreading while they grow; these trees are 19 months old. |
- Posted by applecrazy none (My Page) on Sun, Apr 28, 13 at 17:53
Wow (@Brato) that is a lovely tree. I have some seedling at home. I live in the Caribbean, SAINT LUCIA to be exact. The oldest one grew very slowly and now at five months it is starting to grow rapidly. |
- Posted by foolishpleasure 7B (My Page) on Mon, Apr 29, 13 at 3:32
Apples needs minimum of 400 chill hours just like apricot. So you can not grow apple or apricot or peach. We can not grow Mango or Guava or dates. That is the nature law. |
Foolishpleasure, this is not true. The above apple orchards in Zambia and Rwanda get zero chilling hours, as it never gets below 45 degrees in either location. |
@ applecrazy you are coming along they take off faster after they have been planted out, give the roots space to really spread. I have mad a video of another one http://www.youtube.com/watch?v=nz1iDcIHHqc&lc=-hohSGXBe3MOD_cf1zwNSF3IrViQfF_5FlL1mBu4LKQ you can check it out |
- Posted by macmanmatty2 8b (My Page) on Thu, May 2, 13 at 5:45
I was given scions from an apple that was found growing and fruiting in puerto rico. I was told that it never goes dormant there. But the scions he were dormant. So it must go dormant in ohio where he's from (it would probably die otherwise). If what he told me is true how does he keep it from getting killed? it must be really low chill or no chill to grow and fruit in puerto rico where the lowest temp is about 60 in the winter. I would think that the first few days above 45 it would try to leaf out then get burned in the cold where he lives in ohio? I have plenty of trees grafted so I will report back on how low chill it really is. |
Apples can adapt to the location they are planted in. Anna is an extremely low-chill apple, but Richard Fahey grows in upstate NY and says it acts like a fall apple there, waiting until April to leaf out with all the other trees, while it leafs out in January here. He says it is certainly hardy, but the fruit quality is only so-so. I know Anna is also grown in Ohio. On the other hand, many high-chill apples adapt well in the tropics, one of the most popular being Rome Beauty. |
- Posted by kittymoonbeam 10 (My Page) on Thu, May 2, 13 at 21:00
Applenut, that's an amazing thing that a man can send his kids to school by simply planting some apple trees. It makes me appreciate my apples even more. Thank you for helping them have a better life through apple planting. I will be thinking good thoughts for those Rwanda trees to prosper. |
- Posted by MrsNichole none (My Page) on Sun, May 19, 13 at 13:57
Hi, I'm in Jamaica. I know I won't be able to import any seedlings. Do you know which, if any of the apples that are sold in supermarkets here regularly (red, gala, fuji, globe, pink lady, golden, granny smith) can grow from the seed? Really hoping to be able to grow some apples here even if they're small or not sweet or whatever it would be just for me and my family. Thanks. MrsNichole |
- Posted by guaposhutter none (My Page) on Wed, Jun 12, 13 at 10:38
hey im andel from Trinidad in the Caribbean, im 20 years old, ever since i was a kid Ive been envious of the fact that colder countries could grow apples and we cant, now that im seeing it is possible, i will like to know what kind can grow from the seedlings itself? we usually have fiji, granny smith, red delicious,gala and golden apples here ^_^ |
I grow over 800 varieties of apples in a mild Winter California coastal climate with medium chill at night but mild to warn days in Winter. Only Anna and Dorsett golden are true low chill and bloom in January. A good number of varieties are doing well but a small percentage leaf out late or not at all. So not all apples adapt to low chill conditions. Your best bet is to go with the tried and true ones such as Anna, dorsett golden and Rome beauty. |
I grow over 800 varieties of apples in a mild Winter California coastal climate with medium chill at night but mild to warn days in Winter. Only Anna and Dorsett golden are true low chill and bloom in January. A good number of varieties are doing well but a small percentage leaf out late or not at all. So not all apples adapt to low chill conditions. Your best bet is to go with the tried and true ones such as Anna, dorsett golden and Rome beauty. |
- Posted by nightnurse21 (My Page) on Wed, Oct 9, 13 at 10:11
Hi guys I'm from Barbados and love this thread so please keep it going,for those in the Caribbean that got the apples to sprout and grow what method did you use ? |
Night nurse; You can Google "Growing Apples in the Tropics". |
- Posted by SpiceIslander (My Page) on Wed, Jan 29, 14 at 21:27
I understand that Trinidad is growing Anna and Dorset Golden successfully. I have a phone number that was published in one of the newspapers a couple of years ago for people to order trees. Brato, I also live in Grenada and would love to talk to you about your apple trees. I know that there are also a few people here growing strawberries successfully. |
SpiceIslander that's very interesting news, someone has promised to send me some sion wood of Anna and Dorset Golden, If they make good on that promise then I might be growing them here also. And I also grow strawberries and many other exotic fruits. But if possible can we pursue the option in Trinidad? |
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StarCraft: Burning Ground Tech Demo v0.5
Feb 2, 2014 Demo 20 commentsStarCraft: Burning Ground Tech Demo v0.5 Version. (A Mod for Starcraft: Brood War)
Kel-Morian Combine, after being bombed, destroyed and desolated, found at Mar Sara and Chau Sara a very unique mineral by bombard and captured Sara System stationing some soldiers and manpower to mine the minerals.
After capturing Sara System's Planet, they secretly founded a anti-dominion force, supporting other anti-dominion forces at the same time. Umojan Protectorate hating Dominion also started to support Kel-Morian secretly.
Somehow, dominion got to know it and they sent Ghost Agent to Moria, the capital, and installed a Psi Emitter at the very central underground of the city.
As a result, Moria started to be attacked by Zerg and fell in panic. Quarantine Zones will be established, international trade was canceled and stock market was collapse.
Kel-Morian Combine had already given up their frontier planets because of financial and decreasing production of mines and it became a very dangerous situation after Zerg's attack.
Finally, Kel-Morian Combine's and Morian Miner combine's leader,
General Mah Sakai, founded out that Dominion caused this problem and he let his new order to pass the law quietly.
It was a plan to resist the dominion, the operation was called "Burning Ground"
Likewise, Dominion also started to act against it the same way, just as eye for an eye and a tooth for a tooth.
It was a fight of money, there was mercenaries on the battlefield instead of their own soldiers.
Shortly after that, it came to 2nd Guild war.
It is a war of mercenaries, who became blind to all but money.
Warning : KMC Computer AI Crash
1 comment by Black_Overseer on Feb 2nd, 2014
Starcraft : Burning Ground(A Mod for Starcraft: Brood War)
Requirments: StarCraft Brood War Version 1.16.1
This Mod is Demo version.
if you find bug or something like that, contact me.
Please setting starcraft version to 1.16.1
MERC Project
StarCraft: Burning Ground
Team NOVA INTERACTIVE
Director & Lead Programmer
The Best(firstname.lastname@example.com)
Plugin Programming
Plugin Advise
Selshas Ranos
AI Programming
ssa3233, Lewis(rlaeodhks80)
UI Interface and Launcher
Music from Xenogenocide!
and World War III: Black Gold, MaestroRage
Special Thanks
JabroniJim & Pr0nogo
moonsoldier, Kiith-Somtaaw
Atrox RTS
Thank you for playing this mod!
known issue & bugs
KMC playing as Computer can't do anything (AI Problem)
KMC Computer Ai Crash. don't select KMC Computer!!
KMC Sentinel Damage display tooltip fixed.
and many bugs fixed.
what's new?
Plugin v0.5b list
Dominion Devil Dog(Firebat) Incendiary Burst added, Ignites enemy infantry groups in the bunker.
Dominion Death Heads Reaper Jump Pack plugin added.
non-mining/idle worker plugin added.
KMC Mongoose parabola bullet plugin added.
(automatically slows down the acceleration and top speed of projectile according to the distance between units and targets)
Selecting a Mongooses or KMC Super unit Oppressor or Siege Breakers in siege mode will display the outline of their attack range in large circles,
and You can now view a unit's weapon range by looking at the weapon damage tooltip in the unit console.
Clicking on a factory building displays its rally point. This works only on your own buildings unless you are viewing a replay.
Hero Building System plugin and Package System added. - Dominion Experimental Gate and KMC Experimental Starport
KMC and Dominion Support Power plugin added - KMC Mongoose Airborne, KMC Bomber and Dominion Gunship.
Packages count will be recharged after 4 min.
You can call a bomber and gunship to crush target site, oh mongoose too.
v0.5. New Unit/Bldg list
KMC Command Center is changed, KMC Iron Stronghold.
KMC Science Vessel deleted, NEW Unit KMC Icarus Knight UAV added.
KMC Pirate Capital Ship deleted, NEW Unit Kimeran Juggernaut added.
KMC Griffon is back.
KMC Super Unit Starbase Bomber deleted, NEW Super Unit MAAP "Oppressor" added.
Dominion Experimental Starport changed, Dominion Experimental Gate added.
v0.5. Balance change list
KMC Centurion Reduced HP from 125 to 100, Cost Increased from 100m to 125m / 35g.
and Refraction Laser, Each attack strikes up to one additional target dealing reduced damage.
Dominion Thor Cost reduced from 300m / 200g to 300m / 175g.
Ground Attack Increased from 35(x2) to 40(x2)
Air Attack Increased from 7 to 15
KMC Valkyrie Tech changed, Required Armory tech.
Ground Attack Deleted, Air Attack Splash Deleted, Air Attack Increased 6 to 20, Range Increased 8 to 14
Reduced HP Increased 150 to 200, Cost Increased from 150m / 75g to 250m / 125g.
Build time reduced from 37.5 seconds to 31.25 seconds.
Dominion Super Unit Loki Cost reduced from 2000m / 3500g to 1000m / 1500g.
and Yamato Gun range reduced from 20 to 16, Yamato Gun Splash Deleted,
Shrike Turret deleted.
and some etc balance fixed.
StarCraft: Burning Ground Tech Demo v0.5 Version. (A Mod for Starcraft: Brood War)
Burning Ground EUD Test Map made by. SimMA(dh_eogus)
StarCraft Brood War Color Patch & Fix (Win7 - 32 & 64 bit)
Burning Strike Beta (Mutiplayer only map, co op, team play)
StarCraft: Burning Ground Tech Demo v0.4b Version. (A Mod for Starcraft: Brood War)
StarCraft: Burning Ground Tech Demo 0.3b Version. (A Mod for Starcraft: Brood War)
Maybe you will remake all the other factions or make another SCBW-HD mod?
protoss race is tal'darim
zerg race going to be dominion merc.
move speed increased.
multilock plugin failed.
This mod looks good has different skins/models. Better have at least a LAN/Multiplayer in it.
Promote StarCraft: Burning Ground on your homepage or blog by selecting a button and using the HTML code provided (more).


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Dump Cake
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1 can (20 ounces) crushed pineapple 1 box (16 ounces) yellow or white cake mix 1 can (20 ounces) cherry or peach pie filling 1 stick margarine 3/4 cup pecans, chopped
Start by draining can of pineapple in strainer. Then spread pineapple in a 9x13-inch cake pan that has not been greased. Spread the pineapple evenly. Spoon the pie filling over the pineapple and spread evenly. Dump the cake mix (dry) over the fruits and spread evenly. Slice margarine in small chunks and place all over the cake mixture. Sprinkle the chopped pecans all on the cake. Bake the cake at 350 degrees for 50 minutes. Serve with Cool Whip warm or serve it plain.
Jane Franks Erie
Too-Easy Truffles (More recipes)


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February 2014
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New and renovated hotels are opening and the high-tech convention center is celebrating its fifth anniversary.
That's the message from Ana Maria Viscasillas, president and CEO of the Puerto Rico Convention and Tourism Bureau. "We've been through one of the worst economic maelstroms in history," she said in a recent interview. "But I often say that with challenge comes opportunity, and I truly believe that as the economy continues to improve, we will be stronger for the storm we've weathered."
There are nearly $2.1 billion in development and $350 million in renovation and expansion projects under way or just being completed, including the 156-room W Retreat & Spa–Vieques Island; the five-star Bahía Beach, St. Regis Resort, slated to open in November with a Robert Trent Jones Jr. golf course and a Jean Georges restaurant; the $172 million, 148-room Regent Puerto Rico Beach Resort & Spa; the JW Marriott Hotel & Resort at Coco Beach, featuring 197 guest rooms and a 174-room condo-hotel; the Mandarin Oriental, which is developing a 142-room luxury hotel property complete with a signature spa; and the 503-room Sheraton Puerto Rico Convention Center Hotel & Casino, which opened in November 2009 and serves as anchor hotel to the Puerto Rico Convention Center.
The 580,000-square-foot convention center, meanwhile, is celebrating its fifth anniversary. Completion of the surrounding District in 2012 will provide a dynamic center of office space, retail operations, restaurants, entertainment, and additional hotels, including the 300-room Marriott Courtyard Convention District Hotel, a 252-room Aloft property from Starwood, plus an expected 500-room property, and a 200- to 250-room hotel.
Just outside of the Convention Center District, the Vistas de San Juan hotel project will bring a 48-room corporate hotel to the former Clarion property, along with 197 condo-hotel rooms. A second phase includes a two-tower, 480-room property with more than 300,000 square feet of commercial space. Also just outside of the Convention Center District is the newly rebuilt 184-room Doubletree by Hilton San Juan (formerly the Pierre Hotel), featuring four meeting rooms, a business center, wireless Internet, and other top amenities to cater to the business traveler. In the Condado area, the re-launch of the 570-room Conrad San Juan Condado Plaza followed a $70 million redesign that included refurbishment of all rooms and 40,000 square feet of meeting space. Nearby, development continues at the Condado Vanderbilt Hotel, which is scheduled to open in November 2010 featuring 305 guest rooms and 15,000 square feet of flexible meeting space.
Last year, in reaction to the "AIG effect," Puerto Rico launched its "Not a Boondoggle" promotional campaign. "We want to demonstrate to planners that the island's services, amenities, and business infrastructure make us a serious meeting destination," says Viscasillas. "The campaign also highlights the attributes that make delegates excited about working in a vibrant, sophisticated meeting destination."
Getting There
American Airlines has added daily flights to Puerto Rico from Boston, Chicago, and Washington/Dulles recently, in addition to a new weekly flight from Los Angeles. Passports are not required for U.S. citizens to enter Puerto Rico, as the island is a U.S. territory.


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Direct-drive turbines to propel offshore wind
With projected growth in offshore wind farms, turbine makers are adopting direct-drive generators, a technology that could help address concerns over cost and reliability of offshore wind.
Siemens Energy's first direct-drive turbine is now available for both onshore and offshore applications, the company said last week. Siemens has been testing a prototype since last year and the results have been good, according to the company.
Rated at 3 megawatts of capacity, the SWT-3.0-101 is a lower height and weight than Siemens' 2.3-megawatt machine and has half the number of parts. It's more compact than generators that … Read more


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The bondage of the will'The Bondage of the Wills' is fundamental to an understanding of the primary doctrine of the reformation. In these pages, Luther gives extensive treatment to what he saw as the heart of the gospel. |
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User Review - Elisabeth Sepulveda - GoodreadsI like Luther's emphasis on continual dependency and necessity for grace, as well as his view of the will (which is definitely similar to Augustine, although he recognizes sin as an active force of ... Read full review
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Reformation Theology: Martin Luther on the Bondage of the Will
Let me add just one more quote that shows that Luther was not only convinced of the bondage of the will, but that he was also convinced that a proper ...
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De Servo Arbitrio �On the Enslaved Will� or The Bondage of Will
of the immortal Luther on the Bondage of the Will to the Public. And he trusts he has a sincere. desire, that his own labour may prove to be, ...
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1 BOOK CRITIQUE: THE BONDAGE OF THE WILL by Brian K. Hargrove
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Union Contracts
Columbia University maintains collective bargaining agreements with 13 bargaining units. These agreements set forth the wages, terms and conditions of employment, and working conditions of approximately 3,200 support staff. Contact your union representative.
University-provided benefits are described in Benefits in Brief. However, some unions provide health insurance and pension benefits for their members using funds contributed by the University. Members of these unions should contact the benefit administrators of their benefit plans for information about coverage and benefits.
Attachment | Size |
1199 SEIU United Healthcare Workers East SSA Area | 262.46 KB |
Local 2110 UAW - 2007-2012 DRAFT | 199.2 KB |
Local 241 TWU Maintenance | 921.92 KB |
Local 241 TWU Security Officers | 728.81 KB |


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Learn the basics of playing a forehand shot in the first part of our tennis tutorials with Liam Shelbourne.
Liam Shelbourne is a professional tennis coach (LTA Qualified, BTCA Approved) with Hoole Lawn Tennis Club and Ace Tennis Academy.
In these short video tutorials Liam covers the basics of tennis, including the forehand, backhand, serving and volleying.
For further details about coaching, Liam can be contacted HERE
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Is there something happening in your area you would like us to report on? Get in touch.
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PMC Brass .223, $5.99/20 & $5 shipping for orders $99 or more.
Last edited by widgit; 10-08-2012 at 01:14 PM..
PMC bronze shoots perfectly out of my AR (bushmaster) The only jam I ever experienced using it was from a casing that didn't fully eject before the bolt closed on it.
Their casings are always super clean compared to other brands I've looked at where the brass looks dirty and blemished. Not sure if that really matters, but to me it feels like there is more quality care in the making of pmc's ammunition. Great range ammo |
Great deal! I bought 1000 rounds last time they had this price and they shipped it quickly!
Don't forget if you have a Cabela's VISA, you get FREE shipping and 3 to 5x the points. If you don't have a Cabela's VISA...what is wrong with you!?!?!? Naw, but you can get an extra $25 off any order if you open an account with them. |
I've only been through about 500 rounds of this ammo but it seems to be perfectly suitable brass for plinking, no complaints. You're not going to do much better than this for brass, and you could do a lot worse. 50 boxes will come as a case pack. Last edited by FranksAndBeans; 10-08-2012 at 02:40 PM.. |
Hey, just wanted to say thanks for this post!
Just this past Friday, I bought 1000 rounds of this stuff at the 'sale' price of 6.49/box + 5 shipping (Even at that price, it was a far cry from the last case of 62gr steel core xtac I snagged for .28/rnd a year ago) At any rate, after seeing this post and the sale price back down to 5.99/box I called them up and had them credit me the $25 difference (bringing price per round w/shipping to ~.30/rnd) Thanks again |
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How a college president toppled the ivory tower
Pine Manor College was once a haven for privileged white women. Now its seeing a surge in low-income and minority students.
Chestnut Hill, Mass.
To pay for her education at a public university in New Jersey, Gloria Nemerowicz worked in a bakery, a meat market, and a dry cleaning shop.
Skip to next paragraphSubscribe Today to the Monitor
Her immigrant parents hadn't gone to college and thought her desire to do so was a bit strange. But as long as it didn't cost them anything, they were fine with it.
That's one reason Dr. Nemerowicz radiates respect for students who must pinch every penny or tread an unfamiliar path as the first in their families to go to college.
The young women at Pine Manor College, located on a bucolic campus on the outskirts of Boston, call Nemerowicz by her first name – something she encourages even though she's president of the college. Long a haven for privileged white women, under her leadership Pine Manor has been transformed into one of the nation's most diverse liberal arts colleges.
Students hail from Haiti and Maine, Boston and Mexico. Their SAT scores and grades are usually just average, and more than 60 percent qualify for low-income federal Pell Grants.
But at Pine Manor, and dozens of small colleges like it around the country, students are supported and valued for the life experiences they bring to the classroom.
Now Nemerowicz is drawing on her experience here to answer President Obama's call to restore the United States as a world leader in producing college graduates, particularly by raising the number of degrees earned by underrepresented groups.
"The need for us to find solutions to really grave problems in the world ... means that we can't waste talent," she says. "The work of increasing graduation rates in this country – that's what's burning in my heart."
Only 18 percent of African-American and 12 percent of Hispanic adults have completed four years of college, compared with more than 30 percent of white non-Hispanics, according to a recent report by the Lumina Foundation. The foundation's goal is to help raise the percentage of US adults with college degrees from 39 percent today to 60 percent by 2025.
Colleges like Pine Manor can play a big role in meeting that goal, Nemerowicz says. It's the kind of work they've been doing – somewhat invisibly – for years.
Last spring, she held a "Yes We Must" summit at Pine Manor, bringing together presidents from 11 small liberal arts colleges and education groups that serve low-income and minority students. They shared ways to make college more affordable and raise graduation rates – and launched a Yes We Must coalition at about 100 schools.


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Quick Facts
- NAME: Mel Blanc
- OCCUPATION: Film Actor, Television Actor, Comedian, Musician, Radio Talk Show Host, Talk Show Host
- BIRTH DATE: May 30, 1908
- DEATH DATE: July 10, 1989
- PLACE OF BIRTH: San Francisco, California
- PLACE OF DEATH: Los Angeles, California
Best Known For
Mel Blanc was a voice actor and entertainer who provided voices for Bugs Bunny and over 400 other cartoon characters.
Think you know about Biography?
Answer questions and see how you rank against other players.
Play NowMel Blanc. (2014). The Biography Channel website. Retrieved 03:38, Mar 12, 2014, from http://www.biography.com/people/mel-blanc-9214759.
Mel Blanc. [Internet]. 2014. The Biography Channel website. Available from: http://www.biography.com/people/mel-blanc-9214759 [Accessed 12 Mar 2014].
"Mel Blanc." 2014. The Biography Channel website. Mar 12 2014, 03:38 http://www.biography.com/people/mel-blanc-9214759.
"Mel Blanc," The Biography Channel website, 2014, http://www.biography.com/people/mel-blanc-9214759 [accessed Mar 12, 2014].
"Mel Blanc," The Biography Channel website, http://www.biography.com/people/mel-blanc-9214759 (accessed Mar 12, 2014).
Mel Blanc [Internet]. The Biography Channel website; 2014 [cited 2014 Mar 12] Available from: http://www.biography.com/people/mel-blanc-9214759.
Mel Blanc, http://www.biography.com/people/mel-blanc-9214759 (last visited Mar 12, 2014).
Mel Blanc. The Biography Channel website. 2014. Available at: http://www.biography.com/people/mel-blanc-9214759. Accessed Mar 12, 2014.
Mel Blanc was born on May 30, 1908 in San Francisco, CA. He was a multi-instrumentalist musician and started doing voices on his radio program which couldn't afford additional actors. In 1937, he joined the Warner Brothers animation unit which produced the Looney Tunes cartoons. He created voices for about 90 percent of Warner characters, including Bugs Bunny and Daffy Duck. Blanc died in 1989.
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Intel® Math Kernel Library (Intel® MKL) includes the optimized DFT transform functions on Intel® Xeon Phi™ coprocessors. These functions are carefully vectorized and threaded to take advantage of the hardware features. This article provides some performance tuning tips on running MKL DFT function on Intel Xeon Phi coprocessors. We will start with some simple example code.
Building the example code


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by Baz777, Stock Photo 7901664
amusing, analyst, comical, consultant, couch, doctor, funny, health, healthcare, humorous, humour, joke, medical, moses, patient, practitioner, psychiatric, psychiatrist, psychiatry, psychology, psychotherapist, psychotherapy, quack, shrink, silhouette, tablets, witty
Made with love in NYC.
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Welcome to Design is Kinky...
Bringing you design and art goodness from Sydney, Australia since 1998!
We hope you enjoy and are inspired by the links and other info on the site. Thanks for visiting!
1 more day for submissions to SP Book 2014, Theme: "Evolution' closes end of the day tomorrow – 15th Jan. This work is by Eve Young (SP Book 1 – 2013)
posted By MattPhotographer / Artist Stef Mitchell has some beautiful thought provoking work on her portfolio site.
posted By MattAndy Warhol by Christopher Sauve who has just launched his MADMAUS series at the gallery Hotel Particlulier in SOHO, NYC.
posted By MattDesign is Kinky contributor and all-round talented guy, Mr Chuck Anderson has a new podcast where he chats to creative people doing interesting work. Have a listen!
posted By AndrewFancy checking out a show on Wednesday? The Tate in Sydney have a show opening of new work by Steve Tierney. Head down if you can.
posted By AndrewEver wanted to rebrand a whole country? Stockholm based agency Söderhavet recently rebranded Sweden. Yes, the country. No pressure of course…
posted By AndrewFor the School kid inside everyone, the guys at Komboh have created some super fun posters available for purchase.
posted By MattMADE Quarterly have just released their latest issue. It not only has an awesome cover but also some great content. Grab yourself a copy.
posted By AndrewThe Critical Slide Society is an International surf lifestyle brand designing and manufacturing award winning apparel and accessories for distribution across the best stores in Australia and the globe. We've grown significantly over the past 2 years and we need a super graphic design nerd to work with us to manage the design demands and to work with us to develop the graphic language of the business.
The ideal applicant will have 3+ years experience working as a graphic designer within the fashion or retail industry, and be super passionate about pop-culture (significant surf-culture knowledge and nerd'ism will be favourably looked upon) and current trends in design. More information here.
posted By Andrew'Groupies' a Brand New, Limited Edition, Signed Print for Saul. Grab one for Xmas!
posted By Andrew


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TIL Apple used 'Carl Sagan' as a codename for a computer in the 1990s. Carl Sagan sent Apple a cease-and-desist letter, and Apple changed the name to 'Butt-Head Astronomer'. Carl Sagan sued for libel and lost, and Apple changed the name to 'Lawyers are Wimps'. (en.wikipedia.org)
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Neediest Cases Make a Donation
CHARLESTON, W.Va. - It is hard to imagine that this 35-year-old mother of two has worn dentures for so long that she has worn them out.
A lifetime of poor dental care has taken its toll. The dentures she has been wearing have broken several times, and re-gluing them isn't going to work much longer, the family's caseworker said.
"The dentures give her mouth sores because they don't sit in her mouth right," the caseworker said. The poor condition of the dentures also impairs her speech.
The single mother manages to work a full-time job, although it pays only minimum wage.
"She's a hard worker," the caseworker said.
She brings home about $1,200 a month, and she is eligible for $398 in food stamps. The father of the children, who are 10 and 12, does not provide support.
The mother puts the two girls' needs before hers, the caseworker said.
"The mother really does try hard, but there's just not a lot extra," she said. "They don't have a lot, but they're very appreciative of what people give them."
Make a Donation
1001 Virginia Street East
Charleston, WV 25301


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This TV series ran for 26 episodes.
The series included computer graphics.
Based on Ryu Fujisaki's 1996 manga in "Shonen Jump," itself adapted from a Chinese ghost story.
Despite being ostensibly set in the 11th century, the series depicted a hi-fi setup during festivals.
Battle Of The Titans Production Information |
Production Notes:
Alternate Title:
"General Fun-Faze Vs Commander Wenzon."
Alternate Series Titles:
"仙界伝 封神演義," "Sendai Den Houshin Engi," "Senkaiden Hôshin Engi," "Soul Hunter," "Immortal Tales Of Hoshin Engi," "Soul Hunter - Battle of the Immortals," "Fengshen Yanyi."
If you know of more people who worked on this cartoon, or want to submit additional production information about Battle Of The Titans, please submit your information here.
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Safari Adventure
If your guests think they have been to every type of event or celebration, prove them wrong! Your party adventure includes the Garden Tent, Lecture Hall, Asian Forest, African Savanna, Safari Plaza (outdoors), PPG Aquarium, and Water's Edge.
Maximum Number of Guests
April thru October
(evenings only)
Rental Rate
$5 per guest
(minimum of 2,000)
Animal exhibits and rides close at dusk
All evening rentals are in four-hour increments.
One extra hour may be added at an additional fee.
Outdoor rentals must end by 11 p.m.
All animal exhibits close at dusk
For reservations, call 412-365-2536
For catering information, call our own
"Taste of the Wild Catering" at 412-365-6003.


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UK breakdown cover
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Call us on 0800 085 2721
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High quality, budget accommodation ideal for both families and business travellers. Spacious, en suite bedrooms feature tea and coffee making facilities, and Freeview TV in most hotels. Internet acces...
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Set above the cliffs of Pentire Headland, looking out across the mighty Atlantic, guests are guaranteed amazing coastal views. There are ten bedrooms that were designed by Guy Bostock. They are modern...
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Many guests return to this friendly, family-run house where hospitality and service are noteworthy. Carrek Woth takes its name from the Cornish for Goose Rock, which can be seen in Crantock Bay. All t...
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Tregellist Farm is located in beautiful countryside close to Port Isaac, and convenient for Lanhydrock, Pencarrow, the Lost Gardens of Heligan, Padstow and the Eden Project. All bedrooms are en suite,...
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This 18th-century former posting inn retains many original features and provides comfortable accommodation. Bedrooms are located in both the main house and adjacent coach house - all provide the same ...
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The Bugle Inn a warm and inviting establishment, with its origins dating back to the 17th century, situated in the heart of the Cornish Countryside. Located on the A391 between Bodmin and St Austell, ...
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WTOK Mobile Site
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MSU's Johnson Takes National Award
Posted: 1:05 PM Oct 28, 2013
Reporter: MSU Media Relations
After hauling in a career-high four passes for 70 yards and scoring the game's first touchdown on a 60-yard bomb, Mississippi State junior Malcolm Johnson has been tabbed as the College Football Performance Awards Tight End of the Week.
Johnson, who hauled in the long ball from Dak Prescott, put the Bulldogs ahead of Kentucky 7-0 nearly four minutes into the contest last Thursday night and helped MSU to a 28-22 victory, the program's fifth-straight triumph against the Wildcats. The win pushed Mississippi State to 4-3, and the program owns back-to-back campaigns with a winning record through seven games for the first time since 1999-2000.
The touchdown reception in the first quarter was Johnson's first of the season and the second-longest pass play of the season for MSU. Three of Johnson's four catches versus the Wildcats were good for first downs.
This season, the Tuscaloosa, Ala., native stands third on the team with 14 receptions for 191 yards for an average of 13.6 yards per grab.
Joining Johnson on the CFPA awards this week is junior wide receiver Jameon Lewis, who caught a touchdown pass, ran for a ground score and threw a TD pass during Thursday night's game against Kentucky.
Johnson, Lewis and the Bulldogs return to action Saturday at No. 14 South Carolina. The game is slated for an 11:21 a.m. CT kickoff and will be broadcast on SEC TV.
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Monday, February 8, 2010
© Copyright 2014
Gwinnett Daily Post
LAWRENCEVILLE -- After a year of ups and downs in tough financial times, Chairman Charles Bannister said he will give a "hopeful" message in his State of the County speech Wednesday.
Bannister not only gave two state of the county speeches in 2009, but he twice proposed tax increases, an item that eventually passed and gave officials the ability to fully fund services such as police protection.
Bannister said one or two new initiatives could be announced during Wednesday's speech, given during an annual Chamber of Commerce and Council for Quality Growth luncheon.
According to a press release, Bannister will discuss his vision for 2010 as well as review the achievements of 2009 and the citizen-led Engage Gwinnett committee, which will begin a series of public meetings next week on ways to cut the county's budget.
"Gwinnett County is in an exciting time with a future that is promising and hopeful," Chamber President Jim Maran said. "The Gwinnett County Board of Commissioners is working hard to create and maintain prosperity ... We are pleased to welcome and hear Chairman Bannister speak at the State of the County Address."
The 11:30 a.m. event at the Gwinnett Center costs $75 for Chamber members and $100 for non-members. To attend, RSVP by e-mailing Nicole Wright at email@example.com or visiting www.gwinnettstateofthecounty.com.
More like this story
- Proposal on sales tax won't happen this year ( March 29, 2010 )
- Bannister named vice-chairman of ARC ( January 28, 2010 )
- Probe sought in arrest of Ga. commission chairman ( July 2, 2010 )
- Ex-Gwinnett chairman sues sheriff over DUI arrest ( May 1, 2012 )
- Bannister's career hurt by disputes ( October 9, 2010 )


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'Twilight' guys can turn me gay, says 'Californication's' David Duchovny
Duchovny told Empire magazine that there are definitely some male stars he would go gay for and when it comes to "Twilight," he's not picky.
"Oh, and anyone in the 'Twilight' movies," Duchovny said going down his list of stars he'd go gay for. "I don't know any of their names, but all of them. The wolves, the vampires, they're all fantastic."
Robert Pattinson and Taylor Lautner, don't freak out! The Dish Rag gets that he was probably joking, but it still paints a pretty exciting scene, no?
So, who else tops Duchovny's list of men he'd switch teams for?
"I think Woody Allen is one of a long list of men I might go gay for," Duchovny says.
Um, we liked the "Twilight" images much better.
Would you switch teams for the stars of "Twilight"?
Follow Zap2it and TheRealJethro on Twitter and Zap2it on Facebook for the latest celebrity news and buzz.
More 'Twilight' dish:
'Twilight: Breaking Dawn' director Bill Condon to fans: 'I'm stoked'
'Twilight: Eclipse' new pics: Baby vampires, Bella and Edward
'Twilight: Breaking Dawn' taps Oscar winner Bill Condon as director
'Twilight': Cullen clan talks 'Eclipse'
Photo credit: Summit, Getty Images
I think a Parking Ticket would turn him gay..
Cant you totally see him hooking up with bill maher ... bill would definitely be the bottom though
I love David Duchovny. Didnt think he would be able to do another role since the X-files but feel like this one in californication fits like a glove =D
I can only see him as a top.
I'm sure I COULD TURN Robert Pattinson gay!
This kind of thing is difficult for me to figure out. I'm gay and when I hear straight guys saying they would "go gay" for so-and-so, I think, "...so freaking what?" This pops up all the time, and mostly it is a has-been looking for press. But what is the target audience for this kind of thing? It reminds me of some bimbo listing his/her turn-ons, turn-offs and favorite colors. Snore.


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Permanent Neonatal Diabetes Caused by Dominant, Recessive, or Compound Heterozygous SUR1 Mutations with Opposite Functional Effects The American Journal of Human Genetics, Volume 81, Issue 2, 1 August 2007, Pages 375-382 Sian Ellard, Sarah E. Flanagan, Christophe A. Girard, Ann-Marie Patch, Lorna W. Harries, Andrew Parrish, Emma L. Edghill, Deborah J.G. Mackay, Peter Proks, Kenju Shimomura, Holger Haberland, Dennis J. Carson, Julian P.H. Shield, Andrew T. Hattersley and Frances M. Ashcroft Abstract Heterozygous activating mutations in the KCNJ11 gene encoding the pore-forming Kir6.2 subunit of the pancreatic beta cell KATP channel are the most common cause of permanent neonatal diabetes (PNDM). Patients with PNDM due to a heterozygous activating mutation in the ABCC8 gene encoding the SUR1 regulatory subunit of the KATP channel have recently been reported. We studied a cohort of 59 patients with permanent diabetes who received a diagnosis before 6 mo of age and who did not have a KCNJ11 mutation. ABCC8 gene mutations were identified in 16 of 59 patients and included 8 patients with heterozygous de novo mutations. A recessive mode of inheritance was observed in eight patients with homozygous, mosaic, or compound heterozygous mutations. Functional studies of selected mutations showed a reduced response to ATP consistent with an activating mutation that results in reduced insulin secretion. A novel mutational mechanism was observed in which a heterozygous activating mutation resulted in PNDM only when a second, loss-of-function mutation was also present. Abstract | Full Text | PDF (575 kb) |
Channeling dysglycemia: ion-channel variations perturbing glucose homeostasis Trends in Endocrinology & Metabolism, Volume 23, Issue 1, 1 January 2012, Pages 41-48 Jerod Scott Denton and David Aaron Jacobson Abstract Maintaining blood glucose homeostasis is a complex process that depends on pancreatic islet hormone secretion. Hormone secretion from islets is coupled to calcium entry which results from regenerative islet cell electrical activity. Therefore, the ionic mechanisms that regulate calcium entry into islet cells are crucial for maintaining normal glucose homeostasis. Genome-wide association studies (GWAS) have identified single-nucleotide polymorphisms (SNPs), including five located in or near ion-channel or associated subunit genes, which show an association with human diseases characterized by dysglycemia. This review focuses on polymorphisms and mutations in ion-channel genes that are associated with perturbations in human glucose homeostasis and discusses their potential roles in modulating pancreatic islet hormone secretion. Abstract | Full Text | PDF (837 kb) |
Neonatal diabetes mellitus: A model for personalized medicine Trends in Endocrinology & Metabolism, Volume 21, Issue 8, 1 August 2010, Pages 464-472 Siri Atma W. Greeley, Susan E. Tucker, Rochelle N. Naylor, Graeme I. Bell and Louis H. Philipson Abstract Neonatal diabetes mellitus occurs in approximately 1 out of every 100,000 live births. It can be either permanent or transient, and recent studies indicate that is likely to have an underlying genetic cause, particularly when diagnosed before 6 months of age. Permanent neonatal diabetes is most commonly due to activating mutations in either of the genes encoding the two subunits of the ATP-sensitive potassium channel. In most of these patients, switching from insulin to oral sulfonylurea therapy leads to improved metabolic control, as well as possible amelioration of occasional associated neurodevelopmental disabilities. It remains to be determined what is the most appropriate treatment of other causes. The diagnosis and treatment of neonatal diabetes, therefore, represents a model for personalized medicine. Abstract | Full Text | PDF (189 kb) |
Copyright © 2013 The American Society of Human Genetics All rights reserved.
The American Journal of Human Genetics, Volume 92, Issue 1, 131-136, 27 December 2012
Sarah E. Flanagan1, Weijia Xie1, Richard Caswell1, Annet Damhuis2, Christine Vianey-Saban3, Teoman Akcay4, Feyza Darendeliler5, Firdevs Bas5, Ayla Guven6, Zeynep Siklar7, Gonul Ocal7, Merih Berberoglu7, Nuala Murphy8, Maureen O'Sullivan9, 10, Andrew Green11, 12, Peter E. Clayton13, Indraneel Banerjee13, 14, Peter T. Clayton15, Khalid Hussain16, 17, Michael N. Weedon1 and Sian Ellard1, 2, ,
1 Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exeter EX2 5DW, UK
2 Department of Molecular Genetics, Royal Devon and Exeter National Health Service Foundation Trust, Exeter EX2 5AD, UK
3 Service Maladies Héréditaires du Métabolisme et Dépistage Néonatal, Centre de Biologie et de Pathologie Est, Centre Hospitalier Universitaire, Lyon 69677, France
4 Department of Pediatric Endocrinology, Sisli Etfal Educational and Research Hospital, Sisli, Istanbul 34377, Turkey
5 Pediatric Endocrinology Unit, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34394, Turkey
6 Department of Pediatric Endocrinology, Göztepe Educational and Research Hospital, Kadiköy, Istanbul 34730, Turkey
7 Department of Pediatric Endocrinology, Ankara University School of Medicine, Ankara 06100, Turkey
8 Department of Endocrinology, Children's University Hospital, Temple Street, Dublin 12, Ireland
9 Our Lady's Children's Hospital, Crumlin, Dublin 12, Ireland
10 University of Dublin, Trinity College, Dublin 12, Ireland
11 National Centre for Medical Genetics, Our Lady's Children's Hospital, Crumlin, Dublin 12, Ireland
12 School of Medicine and Medical Science, University College Dublin, Dublin 12, Ireland
13 Manchester Academic Health Sciences Centre, University of Manchester, Manchester M13 9WL, UK
14 Department of Paediatric Endocrinology, Royal Manchester Children's Hospital, Manchester M13 9WL, UK
15 Clinical and Molecular Genetics Unit, University College London Institute of Child Health, in partnership with the Great Ormond Street Hospital for Children National Health Service Trust, London WC1N 1EH, UK
16 London Centre for Paediatric Endocrinology and Metabolism, in partnership with the Great Ormond Street Hospital for Children National Health Service Trust, London WC1N 1EH, UK
17 Institute of Child Health, University College London, London WC1N 1EH, UK
Next-generation sequencing (NGS) enables analysis of the human genome on a scale previously unachievable by Sanger sequencing. Exome sequencing of the coding regions and conserved splice sites has been very successful in the identification of disease-causing mutations, and targeting of these regions has extended clinical diagnostic testing from analysis of fewer than ten genes per phenotype to more than 100. Noncoding mutations have been less extensively studied despite evidence from mRNA analysis for the existence of deep intronic mutations in >20 genes. We investigated individuals with hyperinsulinaemic hypoglycaemia and biochemical or genetic evidence to suggest noncoding mutations by using NGS to analyze the entire genomic regions of ABCC8 (117 kb) and HADH (94 kb) from overlapping ∼10 kb PCR amplicons. Two deep intronic mutations, c.1333-1013A>G in ABCC8 and c.636+471G>T HADH, were identified. Both are predicted to create a cryptic splice donor site and an out-of-frame pseudoexon. Sequence analysis of mRNA from affected individuals' fibroblasts or lymphoblastoid cells confirmed mutant transcripts with pseudoexon inclusion and premature termination codons. Testing of additional individuals showed that these are founder mutations in the Irish and Turkish populations, accounting for 14% of focal hyperinsulinism cases and 32% of subjects with HADH mutations in our cohort. The identification of deep intronic mutations has previously focused on the detection of aberrant mRNA transcripts in a subset of disorders for which RNA is readily obtained from the target tissue or ectopically expressed at sufficient levels. Our approach of using NGS to analyze the entire genomic DNA sequence is applicable to any disease.


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Leo Jerome "Buzz" Rowan
Leo Jerome "Buzz" Rowan, 88, of Silver Bay, formerly of Duluth, passed away Sunday, Jan. 22, 2012.
SILVER BAY - Leo Jerome "Buzz" Rowan, 88, of Silver Bay, formerly of Duluth, passed away Sunday, Jan. 22, 2012.
Service: 2 p.m. Friday, Jan. 27, in the Salvation Army, 27th Avenue West, Duluth.
Visitation: One hour prior to the service.
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Article Information
Related Articles
- Crystal structure of a new heat-labile enterotoxin, LT-IIb
Crystal structure of a new heat-labile enterotoxin, LT-IIb
Structure, Volume 4, Issue 6, 1 June 1996, Pages 665-678
Focco van den Akker, Steve Sarfaty, Edda M Twiddy, Terry D Connell, Randall K Holmes and Wim GJ Hol
SummaryBackground: Cholera toxin from Vibrio cholerae and the type I heat-labile enterotoxins (LT-Is) from Escherichia coli are oligomeric proteins with AB5 structures. The type II heat-labile enterotoxins (LT-IIs) from E. coli are structurally similar to, but antigenically distinct from, the type I enterotoxins. The A subunits of type I and type II enterotoxins are homologous and activate adenylate cyclase by ADP-ribosylation of a G protein subunit, Gsα. However, the B subunits of type I and type II enterotoxins differ dramatically in amino acid sequence and ganglioside-binding specificity. The structure of LT-IIb was determined both as a prototype for other LT-IIs and to provide additional insights into structure/function relationships among members of the heat-labile enterotoxin family and the superfamily of ADP-ribosylating protein toxins.Results The 2.25 å crystal structure of the LT-IIb holotoxin has been determined. The structure reveals striking similarities with LT-I in both the catalytic A subunit and the ganglioside-binding B subunits. The latter form a pentamer which has a central pore with a diameter of 10–18 å. Despite their similarities, the relative orientation between the A polypeptide and the B pentamer differs by 24° in LT-I and LT-IIb. A common hydrophobic ring was observed at the A–B5 interface which may be important in the cholera toxin family for assembly of the AB5 heterohexamer. A cluster of arginine residues at the surface of the A subunit of LT-I and cholera toxin, possibly involved in assembly, is also present in LT-IIb. The ganglioside receptor binding sites are localized, as suggested by mutagenesis, and are in a position roughly similar to the sites where LT-I binds its receptor.Conclusion The structure of LT-IIb provides insight into the sequence diversity and structural similarity of the AB5 toxin family. New knowledge has been gained regarding the assembly of AB5 toxins and their active-site architecture.
Summary | Full Text | PDF (1321 kb) - AB5 ADP-ribosylating toxins: comparative anatomy and physiology
AB5 ADP-ribosylating toxins: comparative anatomy and physiology
Structure, Volume 2, Issue 3, 1 March 1994, Pages 151-158
W.Neal Burnette
SummaryThe crystal structures recently determined for pertussis toxin, cholera toxin, and E. coli heat-labile toxins promise advances in rational vaccine design and improved understanding of G protein-mediated signal transduction.
Summary | Full Text | PDF (911 kb) - A mutant Shiga-like toxin IIe bound to its receptor Gb3: structure of a group II Shiga-like toxin wi...
A mutant Shiga-like toxin IIe bound to its receptor Gb3: structure of a group II Shiga-like toxin with altered binding specificity
Structure, Volume 8, Issue 3, 1 March 2000, Pages 253-264
Hong Ling, Navraj S Pannu, Amechand Boodhoo, Glen D Armstrong, Clifford G Clark, James L Brunton and Randy J Read
SummaryBackground: Shiga-like toxins (SLTs) are produced by the pathogenic strains of Escherichia coli that cause hemorrhagic colitis and hemolytic uremic syndrome. These diseases in humans are generally associated with group II family members (SLT-II and SLT-IIc), whereas SLT-IIe (pig edema toxin) is central to edema disease of swine. The pentameric B-subunit component of the majority of family members binds to the cell-surface glycolipid globotriaosyl ceramide (Gb3), but globotetraosyl ceramide (Gb4) is the preferred receptor for SLT-IIe. A double-mutant of the SLT-IIe B subunit that reverses two sequence differences from SLT-II (GT3; Gln65→Glu, Lys67→Gln, SLT-I numbering) has been shown to bind more strongly to Gb3 than to Gb4.Results: To understand the molecular basis of receptor binding and specificity, we have determined the structure of the GT3 mutant B pentamer, both in complex with a Gb3 analogue (2.0 Å resolution; R=0.155, Rfree=0.194) and in its native form (2.35 Å resolution; R=0.187, Rfree=0.232).Conclusions: These are the first structures of a member of the medically important group II Shiga-like toxins to be reported. The structures confirm the previous observation of multiple binding sites on each SLT monomer, although binding site 3 is not occupied in the GT3 structure. Analysis of the binding properties of mutants suggests that site 3 is a secondary Gb4-binding site. The two mutated residues are located appropriately to interact with the extra βGalNAc residue on Gb4. Differences in the binding sites provide a molecular basis for understanding the tissue specificities and pathogenic mechanisms of members of the SLT family.
Summary | Full Text | PDF (362 kb) - …more
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Copyright © 1994 Elsevier Science Ltd All rights reserved.
Structure, Volume 2, Issue 1, 45-57, 1 January 1994
Research Article
The crystal structure of pertussis toxin
Penelope E Stein1, Amechand Boodhoo1, Glen D Armstrong1, Stephen A Cockle2, Michel H Klein2 and Randy J Read1, *
1 Penelope E Stein, Amechand Boodhoo, Glen D Armstrong and Randy J Read (corresponding author), Department of Medical Microbiology and Infectious Diseases, 1– 41 Medical Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
2 Stephen A Cockle and Michel H Klein, Connaught Centre for Biotechnology Research, 1755 Steeles Avenue West, Willowdale, Ontario, M2R 3T4, Canada
- Background Pertussis toxin is an exotoxin of the A-B class produced by Bordetella pertussis.The holotoxin comprises 952 residues forming six subunits (five different sequences, S1– S5). It plays an important role in the development of protective immunity to whooping cough, and is an essential component of new acellular vaccines. It is also widely used as a biochemical tool to ADP-ribosylate GTP-binding proteins in the study of signal transduction.Results The crystal structure of pertussis toxin has been determined at 2.9 å resolution. The catalytic A-subunit (S1) shares structural homology with other ADP-ribosylating bacterial toxins, although differences in the carboxy-terminal portion explain its unique activation mechanism. Despite its heterogeneous subunit composition, the structure of the cell-binding B-oligomer (S2, S3, two copies of S4, and S5) resembles the symmetrical B-pentamers of the cholera toxin and Shiga toxin families, but it interacts differently with the A-subunit. The structural similarity is all the more surprising given that there is almost no sequence homology between B-subunits of the different toxins. Two peripheral domains that are unique to the pertussis toxin B-oligomer show unexpected structural homology with a calcium-dependent eukaryotic lectin, and reveal possible receptor-binding sites.Conclusions The structure provides insight into the pathogenic mechanisms of pertussis toxin and the evolution of bacterial toxins. Knowledge of the tertiary structure of the active site forms a rational basis for elimination of catalytic activity in recombinant molecules for vaccine use.


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HAL : in2p3-00640993, version 1 |
arXiv : 1110.0208 |
DOI : 10.1103/PhysRevD.85.022001 |
Fiche détaillée | Récupérer au format |
Physical Review D 85 (2012) 022001 |
All-sky Search for Periodic Gravitational Waves in the Full S5 LIGO Data |
VIRGO Collaboration(s) |
(2012) |
We report on an all-sky search for periodic gravitational waves in the frequency band 50-800 Hz and with the frequency time derivative in the range of 0 through -6e-9 Hz/s. Such a signal could be produced by a nearby spinning and slightly non-axisymmetric isolated neutron star in our galaxy. After recent improvements in the search program that yielded a 10x increase in computational efficiency, we have searched in two years of data collected during LIGO's fifth science run and have obtained the most sensitive all-sky upper limits on gravitational wave strain to date. Near 150 Hz our upper limit on worst-case linearly polarized strain amplitude $h_0$ is 1e-24, while at the high end of our frequency range we achieve a worst-case upper limit of 3.8e-24 for all polarizations and sky locations. These results constitute a factor of two improvement upon previously published data. A new detection pipeline utilizing a Loosely Coherent algorithm was able to follow up weaker outliers, increasing the volume of space where signals can be detected by a factor of 10, but has not revealed any gravitational wave signals. The pipeline has been tested for robustness with respect to deviations from the model of an isolated neutron star, such as caused by a low-mass or long-period binary companion. |
Optique et Photonique APC - ADAMIS |
APC - Cosmologie et Gravitation
Thème(s) | : | Physique/Relativité Générale et Cosmologie Quantique |
Lien vers le texte intégral : |
http://fr.arXiv.org/abs/1110.0208 |
in2p3-00640993, version 1 | |
http://hal.in2p3.fr/in2p3-00640993 | |
oai:hal.in2p3.fr:in2p3-00640993 | |
Contributeur : Nicole Berger | |
Soumis le : Lundi 14 Novembre 2011, 15:51:22 | |
Dernière modification le : Jeudi 28 Novembre 2013, 16:45:03 |


text: Cover picture: The Landscape of Protein Evolution. Protein evolution in nature is subjected to constraints from multiple scales—molecular, cellular, and ecological. Under the selection against the cytotoxic effects of protein misfolding, there exists a surface (represented by the landscape) that relates these multiscale constraints. Similar to a stream flowing in a valley, proteins evolve towards the minima of this evolutionary landscape. See the letter by Serohijos et al. on page L01.


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An indigenous group with a millenarian bond to their land are sitting on large reserves of a precious metal. A massive multinational corporation coming from a foreign land with the intention of getting access to the said metal at whatever cost. A conflict that has left people dead and that has the potential to take even more lives - indigenous lives, of course - destroying the environment in the process.
If the story rings any bells, it is because it does. But you would be forgiven for thinking we are talking about Pandora, and the RDA Corporation's relentless search for unobtanium under the sacred soil of the Na'vi, in the 2009 film Avatar, directed by James Cameron.
As a matter of fact, we are talking about the Minas Conga project in the region of Yanacocha in Peru, and about the Colorado-based Newmont Mining Corporation's persistent attempts at removing the local indigenous communities, and changing and contaminating their ancestral landscape in the process, all to expand their gold extraction operations in the area. Sadly enough, the entire world knows about Pandora, but not very many know about Yanacocha.
The new gold rush
With the beginning of the financial crisis in 2008 gold suddenly became one of the most precious commodities in the international markets. Not surprisingly, this led directly to an increase in gold mining worldwide, and to an expansion of the operations - both legal and illegal - of multinational corporations, often with questionable human rights and environmental records.
In neighbouring Colombia, an estimated 5,000 children are now daily engaged in illegal gold mining works often under the most treacherous conditions. Also in Colombia, former paramilitary groups have now eagerly taken on the extraction of this precious metal as a form of supplementing their drug-related incomes. The situation is not much better in Ecuador, where it is only through the fundraising efforts of international NGOs and individuals that the national park of Yasuni and the indigenous communities that inhabit it, have been spared the destruction that open air mining brings.
The case of Newmont is not unique. Other gold mining companies, mostly with headquarters in the US and Canada, have been repeatedly accused of manipulating local politics, of ignoring the interests of local communities standing in their way, and of destroying and polluting the environment across Latin America and other parts of the world.
Barrick Gold, for example, has been accused of dumping toxic substances directly into the riverine system in Porgera, Indonesia. Although the Canadian-based company has been successful in rebutting local demonstrations, the magnitude of the damage caused to the environment has been so noticeable that the Norwegian Pension Fund felt necessary to exclude the company from their investment plans as a penalty. Another Canadian-based company, Gold Corp, has been at the end of similar accusations in relation to Marlin Gold Mine in Guatemala.
Although Newmont is then hardly an exception, what makes it special is their talent in manipulating public opinion. Over the years, they have dodged public demonstrations and have taken on local politicians and community leaders who have questioned their actions in the area.
Newmont also has dubious records in other parts of the world. In the US, they were accused of taking years of questionable tax deductions from the state of Nevada. In Ghana, their invasive Akyem project, for which they were given the 2009 Public Eye "Hall of Shame" award, led to accusations of the destruction of unique natural habitats, the pollution of soils and rivers, and the displacement and resettlement of people. All facts that Newmont, naturally, dispute.
Minas Conga project
Newmont's involvement in Yanacocha is not new. One of the two largest and more productive gold mines in the world, with a number of open pits across approximately 25,000 hectares of this Peruvian region, the potential of extracting yet more gold in Yanacocha is not to be overlooked.
For almost two decades, Newmont has linked up with Peruvian companies, including Minas Buenaventura, to extract gold from the soil of Cajamarca region, where Yanacocha is located. Daily dynamite blasts loosen the rock, which is then sprayed with a solution of cyanide. The process, predictably, is far from being environmentally friendly, producing a number of contaminant agents, including mercury, cadmium and arsenic.
Although Newmont's own environmental impact assessment states that their projects are not causing irreparable damage to the local ecosystems, their opinion is questionable, especially in light of their previous record in the area.
Back in June 2000, one of Newmont's trucks spilled between 80 and 151kgs of mercury just outside the village of Choropampa. The locals, completely unaware of the danger and thinking they had found some valuable metal, gathered the mercury and took it to their homes. Only a few days later, between 50 and 70 residents, including several children, showed symptoms of mercury poisoning and had to be hospitalised. Newmont, predictably, denied failing to inform the locals about the accident and the danger they had been exposed to.
Newmont also failed, at least initially, to recognise the local population as indigenous in their 1999 environmental impact study. By doing so, they probably hoped to get around Peru's strict legislation protecting indigenous communities from enterprises such as theirs.
These early signs caused the locals to distrust the Newmont and helped to create a state of awareness that was recently revived when the Minas Conga project was approved.
The escalation of the conflict in the past year is a result of a plan to open a new pit with disastrous consequences for the indigenous communities and for the local ecosystem. Particularly problematic has been the idea of drying out four lagoons and replacing them with four water reservoirs, a move that would certainly lead to increasing problems with drinking water supplies and to the extermination of the flora and fauna in the lagoons. There are well founded fears that the soils in the region may never be fertile again and that the contamination may even reach the Maranon river, an important affluent of the Amazon.
Given that, according to Newmont's own assessment, the mining industry has hardly brought any improvement to the locals in the area before, the new project has understandably been received with fierce resistance.
Rather than engaging openly with the indigenous communities, who have lived in the region for millennia, Newmont has lobbied the government, accused local politicians of opposing Newmont's interests, and has even gone so far as to dismiss opposition to the project as emanating mostly from the most uneducated portion of the communities.
The future
The series of demonstrations that began last year against Newmont's Minas Conga project has, for now, ushered encouraging results for the locals. The Peruvian government has recently come to the realisation that the conflict associated with the project is having an adverse effect on local businesses in the area, and has agreed to call it off, at least for now.
A recent poll conducted in the Cajamarca region showed that 78 per cent of the people opposed it. Only a few days ago, Diario Correo, one of the main newspapers of Peru referred to the project as "dead" and "collapsed". It seems that the communities, as in Avatar, have won the first round.
Nevertheless, as in Avatar, sequels are likely to happen. All that gold won't be sitting there for long without greedy multinationals attempting to extract it, even against the will of the local populations. By all the means available to them, they must remain alert for the time when the big machines show up again.
Manuel Barcia is Deputy Director at the Institute for Colonial and Postcolonial Studies at the University of Leeds.
The views expressed in this article are the author's own and do not necessarily reflect Al Jazeera's editorial policy.
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November 6, 2012
Stalemate at Bolton
Goalkeepers Adam Bogdan and Kasper Schmeichel were in terrific form as Bolton and Leicester shared the points in a goalless encounter.
Bogdan primarily frustrated the Foxes in the first half but made a superb second-half save to thwart Anthony Knockaert.
Schmeichel was busier after the break but perhaps his most notable moment was a double save from Lee Chung-yong just before half-time.
After getting off to a winning start against then-league leaders Cardiff on Saturday in his first game in charge, Bolton boss Dougie Freedman named an unchanged starting line-up.
Leicester manager Nigel Pearson made four changes after defeat to Watford. One was enforced, with Zak Whitbread sent off at Vicarage Road, so Manchester United teenager Michael Keane made his debut at centre-back alongside captain Wes Morgan.
Another defensive change saw Ritchie De Laet return from suspension to replace Liam Moore at full-back.
Elsewhere, winger Lloyd Dyer and striker Martyn Waghorn were recalled while 19-year-old midfielder Jesse Lingard, who came from Old Trafford with Keane earlier on Tuesday, was on the bench.
Leicester were frustrated in the early stages by the in-form Bogdan as the Hungarian goalkeeper produced parrying saves to thwart David Nugent, Danny Drinkwater and Dyer.
Bogdan also made a superb save to keep out Waghorn's powerful strike.
Bolton continued to ride their luck as De Laet's shot clipped the top of the crossbar but some late pressure almost saw the hosts take the lead just before half-time.
Lee's long-range shot caused Schmeichel problems, with the Leicester keeper pushing the ball back into the South Korean's path. Lee took another shot but Schmeichel pushed it wide. From the resulting corner, Darren Pratley volleyed over.
Schmeichel was called into action again shortly after the break, this time to keep out Kevin Davies' header.
The Dane denied Bolton once more when he turned a Mark Davies effort around the post for a corner.
Leicester threatened again midway through the second half but Nugent shot into the side-netting following Knockaert's lovely lofted pass.
Bogdan then pushed away a fiercely-struck shot from Waghorn before making a superb save at his near post from Knockaert after Bolton were caught with a short corner.
Schmeichel spilled a shot from Chris Eagles late on but Morgan was on hand to clear away the loose ball and an entertaining clash finished goalless.
Scoring Summary
Bolton Wanderers | Leicester City |
Bolton Wanderers | Leicester City |
1 Adam Bogdan | 1 Kasper Schmeichel |
18 Samuel Ricketts | 15 Michael Keane |
12 Zat Knight | 3 Paul Konchesky |
15 Stephen Warnock | 5 Wes Morgan |
4 Matthew Mills | 2 Ritchie De Laet |
8 Keith Andrews | 11 Lloyd Dyer |
21 Darren Pratley | 10 Andy King |
7 Chris Eagles | 4 Daniel Drinkwater |
16 Mark Davies | 24 Antony Knockaert |
27 Lee Chung-Yong | 35 David Nugent |
14 Kevin Davies | 14 Martin Waghorn |
Substitutes | |
34 Jay Lynch | Conrad Logan 13 |
2 Tyrone Mears | Liam Moore 22 |
5 Tim Ream | Matthew James 16 |
10 Martin Petrov | Ben Marshall 7 |
20 Jay Spearing | Jessie Lingard 23 |
19 Marvin Sordell | Marko Futacs 29 |
17 Benik Afobe | Jeffrey Schlupp 18 |
Substitutions | |
Martin Petrov for Lee Chung-Yong (71) | Jeffrey Schlupp for Paul Konchesky (40) |
Benik Afobe for Chris Eagles (81) | Liam Moore for Michael Keane (57) |
Jessie Lingard for Martin Waghorn (85) | |
Yellow Cards | |
Samuel Ricketts (33) | |
Darren Pratley (42) | |
· Squads: Bolton Wanderers | Leicester City |


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In May, the Ohio Attorney General's Office's Charitable Law section won a default judgment for $3.7 million, plus attorney fees from the U.S. Navy Veterans Association. Assistant Attorney General Dionne DeNunzio proved the organization had falsely claimed to raise money for U.S. veterans' causes; in reality, very little money ever went to help veterans. The U.S. Navy Veterans Association is believed to have collected more than $100 million nationwide since its formation in 2001. Several state attorneys general conducted investigations, but Ohio took the lead in prosecution.
The accused mastermind behind the plot, Bobby Thompson, has been on the lam since June 2010 and remains at large. However, he uses a stolen identity; his true identity has yet to be determined.


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Bond's Newest Girl: Bérénice Marlohe
As if you really needed another reason to see Skyfall—we like her shaken, not stirred.
Does Bronson Pinchot know you raid his wardrobe?
The Worst-Dressed Bond Villains of All Time
A Tribute to the Lesser Bond Girls
A word of warning, ladies. Simply knowing James Bond could get you killed.
When gay marriage was legalized in Spain, Javier Bardem said that if he were gay, he would "get married tomorrow, just to fuck with the Church."
"I've always said I don't believe in God, I believe in Al Pacino." - Javier Bardem
On the Cover: Javier Bardem
Michael Paterniti talks to the gifted chameleon actor and new Bond villain, who happens to look killer in a suit:
The struggles of his mother seem to have left another lasting imprint, persisting for Bardem in an afterimage that still haunts: the unringing phone. "It was black and old, with a rotary dial," he says. "That fucking phone was like a monster, and we waited for it to roar, one month, three months, sometimes a year between acting jobs. Eventually my sister painted it pink. Employment was an issue for my mother from the day I was born. Even for me: Until ten years ago, I couldn't jump from one job to another. You think it's going to be forever—I hope—but each job is just one more chance."
He leans into the brick wall, hugging himself. "It's the actor's insecurity: One day you're liked and the next it's ah, nah, not that guy. My mom told me, 'Just keep doing your job.' You get something gold, thank you. You suck, thank you. You keep on moving."
You suck. Those words make me laugh when he first says them at the restaurant; they aren't exactly the descriptors that come to mind when conjuring Bardem's talent. Bardem occupies an enviable place in the Hollywood constellation—his dignity remains intact—even though, as he points out, his career has been more like an anti-career. ("What career?" he says. "I've always been like, 'We're here now. Let's do this.' ") Where other Spanish actors left home early to try out American film, sometimes filling the token-Latin slot in a cast, Bardem's theory was simple if audacious: Pick great Spanish parts and eventually Hollywood would come calling.


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Lens less than 5.0" (12.7cm) Ikelite SLR Port Chart Of Popular Lenses
Ikelite acrylic domes are injection molded specifically for underwater photography, and represent the finest optics in the industry. Domes are recommended for 28mm and wider lenses.
Zoom lenses that do not focus to 12" (30cm) require a +4 diopter close-up lens (available from camera stores)


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For the first time in three election cycles, Bloomington State Representative Peggy Welch will have an opponent in November's general election.
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Mike Hull
COLLEGE FOOTBALL PSU fills needs with incoming class
NCAA Football By FRANK BODANI Daily Record/Sunday News In the end, Penn State got ..... Pennsylvania, and nationally, in quarterback Paul Jones and linebacker Mike Hull . ---------- Frank Bodani is a writer for the York Daily Record
Warner joins pride of Lions in Hall // Two-time All-American tailback Curt Warner is the 22nd Penn S
NCAA Football Penn State 's football program is well-represented in the College Football Hall of Fame. Seventeen players join five ..... Coxson joins Canon-McMillan linebacker Mike Hull and Sto-Rox quarterback Paul Jones as Lions
PENN STATE FOOTBALL: Lions update recruiting approach
NCAA Football By FRANK BODANI For Public Opinion ..... closer to the Floridas and USCs of the college football world. To understand, consider that ..... quarterback Paul Jones and linebacker Mike Hull . The other front-liner, running
Penn State hangs on, upsets No. 14 Wisconsin 31-24 - College Football News | FOX Sports on MSN
Stave was intercepted by Ryan Keiser in the end zone with 1 second left. ''Both elation and relief,'' linebacker Mike Hull said in describing the moment. ''We made it closer than we had to, but we got the win and that's all that matters
O'Brien, PSU optimistic heading into summer - College Football News | FOX Sports on MSN
getting from veterans like linebackers Glenn Carson and Mike Hull , who played just a couple series before sitting out ..... coaches decide what happens from there.'' GEARING UP College football is finally back. Catch up on spring action. Secondary
Optimistic O'Brien opens Penn St spring practice - College Football News | FOX Sports on MSN
Gerald Hodges and Michael Mauti from last year's team, plus backup Michael Yancich . Glenn Carson is back, and reserve Mike Hull seems primed to ascend to a leading role at Linebacker U. But another returning reserve, Ben Kline , will miss the spring
Penn State football transfer rule set to expire - College Football News | FOX Sports on MSN
the trio of proven impact players in the front seven of defensive end Deion Barnes , and linebackers Glenn Carson and Mike Hull . Coach Bill O'Brien hopes to build on an 8-4 season in 2012, considered a resounding success given the unprecedented
QB, depth concerns will test O'Brien at Penn State
depth concern is at linebacker. Glenn Carson and Mike Hull form a rock-steady base, but after that new defensive ..... 3rd, Big Ten Leaders Division. --- Online: AP college football site: http://collegefootball.ap.org
Joy back in Happy Valley for one day
on PSU Granted, in college football , 10 months isn ..... the coverup. 2012 COLLEGE FOOTBALL Scoreboard | Full ..... Polls On TV: FOX College Football Charles Davis' Power ..... scandal. Later, Mike Hull 's 74-yard fumble
Mauti injury hits Penn State hard
Polls On TV: FOX College Football Charles Davis' Power ..... each week of the 2012 college football season. The win clinched ..... accomplishment that few college football observers thought ..... contests) and linebacker Mike Hull are two bright spots


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Vita Sidorkina by Wee Khim for L'Officiel Singapore March 2012
Life in Mono – Vita Sidorkina wears all white looks for the March issue of L'Officiel Singapore, shot by Wee Khim. Styled by Johnny Khoo, Vita wows in elegant ivory pieces from labels such as Ralph Lauren, Chanel, Givenchy and Dior. A messy bun by hair stylist Dexter Ng and flawless makeup by Peter Khor complete the sophisticated ensembles.


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Published January 25, 2013, 08:46 AM
Blind New Hampshire sled dog thrives with brother's help
JEFFERSON, N.H. (AP) — When Gonzo started tripping over his food dish three years ago, no one could explain or stop the Alaskan husky's quickly advancing blindness. But a veterinarian offered some simple advice: "Run this dog."By: HOLLY RAMER,Associated Press, Associated Press