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Online Courses: I do not take online classes but I am very sure that it is a great experience in every way.
Online Courses: I have not really taken online classes in this college but when compared with traditional school, they seem not to have many classes online. So far, the classes on line is the general classes and it cost more money than classroom courses.The registration process is easy and the professor/student relationship is okay. There is also good peer-to-peer relationship.
Online Courses: i am registered for in class courses. I am sorry I cant answer this because I dont know
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Featured Freeware: doPDF
doPDF is a fairly simple program. Even though it's utterly bereft of features beyond its main function--to let users create PDF documents from within any program using the native Print option--it's still worthwhile because it does the basics very well.
Along with whatever printer you've got hooked up that shows up in the Print dialog box, doPDF adds an eponymous option. Select it, hit print, and you get the chance to change the name of the PDF you're creating. Hit enter again and the PDF gets created, then automatically opens for you to proofread. Lacking any … Read more

subset: commoncrawl


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About Us
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posted 8/31/2011 by
Cyril Lachel
other articles
by Cyril Lachel
First it was Spelunker HD, then Spelunky and now Boulder Dash-XL. It's as if somebody decided that retro-style cave exploration was the happening thing and didn't tell the rest of us. Not that I'm complaining, because it turns out that this reimagined classic is just as much fun as the 1984 original. With updated graphics, nearly a half dozen game modes and an attractive price, Boulder Dash-XL shows the competition a thing or about finding treasure deep below the earth's crust.
The original 1984 game is a genuine masterpiece, a maze game that managed to feel nothing like Pac-Man. This 2011 update isn't nearly the revelation the first game was, but still makes for a fun time at a cheap price. The concept is the same and there are more than enough modes to keep gamers interested for a long time to come. Gamers definitely get their money's worth with Boulder Dash-XL.
This is a game about exploration and diamond collecting. In each level the adorable robot characters (Rockford and Crystal) must push their way through dirt and snow to pick up hidden diamonds and find the exit. Each of the 100 levels has a set number of diamond to track down and collect. Once that number secured, an exit opens up and allows our heroic bots to move on to the next stage. Or, if you're really daring, you can track down every last collectable before moving on.
Of course, all this diamond collecting is easier said than done. Rockford will need to look out for a whole host of tricky enemies, as well large boulders that want nothing more than to squish the robot collectors. The good news is that the player is able to walk in any direction, including up and down. But moving dirt can be tricky, because it will change the enemy paths and send environmental dangers (and even the diamonds themselves) tumbling down. It's up to the player to chart the right path and use the boulders to defeat enemies. If you can avoid enough danger while picking up diamonds, you'll live to see another day and brave the cavern maze again.
Most of the enemies have been brought over from the old computer game, so it's fun for old school gamers to see how their favorite baddie has been remade. Thankfully the game eases you into the challenge, which isn't something I can say about all cave exploration simulators (I'm looking at you Spelunker HD). But don't be fooled by the relative ease of the first dozen levels, Boulder Dash-XL gets tough in a hurry. It's going to take a lot of digging to beat this $10 Xbox Live Arcade game.
The good news is the game is always adding new ideas and obstacles, which forces players to always be on their toes. The even better news is that you can play this game several different ways, completely changing up the rules and objectives. The result is a versatile little action action/puzzler with more content than you'll know what to do with.
On top of the standard campaign, Boulder Dash-XL also includes several ingenious bonus modes. The most interesting is the puzzle mode, which gives players a cavern that can only be completed one way. It's up to the players to figure out the one route, collect the diamonds and find the exit.
The next mode allows players to replay completed stages without the worry of time limits. This Zen Mode is good for gamers wanting to hone their strategies. There's a Score Challenge, which has players scrambling to pick up as many points as possible and compete against their friends via an online leaderboard. These three variations on the theme help to keep the action fresh, though they pale in comparison to the full campaign mode.
The 1984 original is also included in the package, though it's not at all what I was expecting. Instead of emulating an old computer or console, the developers have decided to remake the game using polygons. The result is a little ... weird. The visuals look fine, though they are designed to appear lower-res. I wasn't a big fan of how these two styles mixed, but did have a good time playing through the recreated levels from the original game. Keep one thing in mind: The Retro Mode isn't messing around; it starts considerably harder than the standard campaign.
I was impressed with the game's presentation, especially when it comes to the character models. Swapping from humans to robots seems like an unnecessary change, but everything else was faithfully recreated. The game throws in some really cool lighting effects, the kind of thing you normally wouldn't expect from an otherwise simple puzzle game. The whole game sparkles like the diamonds you can't get enough of.
The controls are also good, though not as flashy as the graphics. Don't be fooled by the simple control scheme, because Rockford has a few tricks up his sleeves. For one thing he can grab on to items. What's more, hardcore players will discover that this robot can dig so fast that sometimes a boulder won't have time to crush him. The game works fine with both the D-pad and the analog stick, which is about all you can ask from this style of Xbox Live Arcade experience.
At a mere ten dollars, Boulder Dash-XL is an easy game to recommend. The simple gameplay, impressive presentation and shocking amount of content makes this a no-brainer, even if you weren't born when the 1984 original was all the rage. It may not be as flashy as Pac-Man Championship Edition DX, but this is one maze game that will stick with you for years to come.
More On:
Boulder Dash-XL
Kalypso Media
* The product in this article was sent to us by the developer/company for review.
Rockford is back and ready to collect more diamonds than ever. With an emphasis on unique modes, this updated classic is dressed to impress. Boulder Dash-XL has enough original content to bring in a whole new generation of spelunkers. This is one cave worth exploring!
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The annual Mother's Day (May 9th) Orchid Show presented by the Peoria Orchid Society at Luthy Botanical Gardens Sunday May 9th.
The Orchid Society members will have a display along with blooming plants that will be available for the public to purchase. Members will give advice about orchid culture and offer repotting. The show is from 12:00 noon until 5:00 pm.
Annual Mother's Day Orchid Show
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Melissa & Doug
Melissa & Doug Grocery Store and Lemonade Stand
5.00 rating Grandparents of triplets by Clarence Sullivan on May 14, 2010
We were very pleased with this product and am sure they will have lots of good times with this. Very Sturdy and made well.
5.00 rating Huge hit even with older kids by momof4 on February 2, 2010
I bought this for my just turned 2 year old daughter. Not only does she love it but so do her big brothers, ages 6 and 9 and her 10 year old cousin. They played with this everyday since I got it and I mean all day long. Great product. Only wish the sign was a little bit taller on the top as like I said the older kids like it, but it's a little low for them and gets in the way when they are ringing up the groceries behind the counter.
5.00 rating Great Product-Suggest a change in the assembly instructions by Leroy Hunt on December 12, 2009
In step 2. Under "note" You mention that the Dowel nuts will fall out if you try and put them in all at one time. If you turn the stand upside down and put the handles on upside down they will not fall out. Also 3 of the bin supports were badly warped. Other than this we love the product.
5.00 rating Fun by pamela Roux on August 7, 2009
This product is a must have for childrens imagination. very sturdy! the kids love it!!!
5.00 rating Great Product!!!! by Andrea Espinoza on June 27, 2009
My kids have used this twice now as a lemonade stand during my garage sales and we have gotten tons of compliments!!!! Many people have asked to purchase it. Only thing I would change would be the height. It could be taller. |


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One thing you perhaps misread is the level of potassium in bananas. A single banana has 450mg of potassium, while a cup of coconut water has about 600mg of potassium. You really shouldn't be drinking this stuff everyday like you would a soda or juice. These levels of potassium could be problematic especially if you have renal disease or are taking stuff like digoxin. Anyways, just my 2 cents. |
"This donut has purple in the middle, purple is a fruit."
A pallette? Am I reading this right? You need a pallette of chocolate covered pretzels? Where the hell am I supposed to... And what is this? A drum of grape jam? What, is that like a drum that they ship oil in? And look at this one! A desk of Cheez-Its. A desk? Where are you getting these units of measurement from? |
I grew up in the tropics with family & we drank LOTS of COCONUTS EVERY DAY (2 to 4) (no joke, they grow wild on the trees & are super cheap there) & still drink on average once or twice a day in Cali & never had any problems with them --instead, they make us feel great. Coconuts contain healthy medium chain triglycerldes as well as lauric acid/monolaurin with anti-bacterial, anti-viral & anti-fungal properties. I'm a athlete/Mixed Martial Arist/bodybuilder with muscle, 8% bodyfat & a 6-pack, rarely ever get sick & my doc is amazed at my blood pressure & HDL levels. Coconut water is MUCH HEALTHIER than fructose juice or hi-fructose corn syrup soda. 6+ month studies by Princeton Medical Center showed that rats given hi-fructose corn syrup gained 48% more fat, especially belly fat, than control group fed other sugars, despite CALORIES BEING KEPT THE SAME FOR BOTH GROUPS, which dispels the MYTH that a 'calorie is a calorie is a calorie' spouted by ignorant people for decades. Also, multiple 2+ year studies by Stanford Medical Center show that those on a hi-protein/low-starch/higher fat diet lost TWICE as much fat & still had healthy cardio levels than those on a low-fat diet. Dozens of other studies showed the same results too Previous studies funded by corn lobbies used short studies that lasted only a few hours to a few days to show that there was "no difference" just like previous studies funded by cigarette makers showed "no difference" in cancer rates between non-smokers/smokers because those studies lasted only a few days to a few weeks also. Unlike fructose juice drinks, coconut water is mostly glucose, which is why it can be & was used as IVs in the tropics by medical doctors. Glucose is metabolized well by all cells in your body for energy but fructose like alcohol is metabolized by your liver easily into belly fat, which is why many who drink lots of alcohol get a "beer belly" or those who drink lots of fruit juice get a pot belly/vegetarian "Buddha belly" I laugh at other experts who recommend hi-fructose corn syrup drinks (sports or soda) or eating pretzels for your sodium, LOL (all the while trying to tell others to cut their salt intake or sugar intake). http://rt.com/usa/food-fructose-o...lucose-230 -how Fructose makes you fatter Last edited by rippedbuff; 03-06-2013 at 12:23 AM.. |
I tasted this last year for the first (and only) time. First, to me it tastes barely (if anything) of coconut, and the taste that is there isn't really a selling point. Second, it's kind of salty, which is strike two. Finally, it's a little slimy. In short, I'll stick with actual water.
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ZICO Pure Premium Coconut Water, Natural, 33.8-Ounce Container (Pack of 6) $19.48 w/S&S and 20% off coupon | DOGGIEVIEW | Deal Talk | 2 | 01-05-2013 11:40 AM |
ZICO Pure Premium Coconut Water: 6-Pack of 33.8-Ounce Natural $17, 12-Pack of 11.2-ounce Mango $14.50, 12-Pack of 14-Ounce Chocolate $17 + More + Free Shipping | aneczka | Deal Talk | 60 | 01-31-2012 02:52 PM |
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ZICO Pure Premium Coconut Water, Variety Pack (Natural, Lima Citron, Pomberry, Pineapple, Mango), 14-Ounce Bottles (Pack of 15) $22, Pure Premium Coconut Water,Natural, 33.8-Oz $22 | Selma | Deal Talk | 5 | 04-27-2011 01:04 AM |
ZICO Pure Premium Coconut Water, Variety Pack (Natural, Lima Citron, Pomberry, Pineapple, Mango), 14-Ounce Bottles (Pack of 15) $19 | Selma | Deal Talk | 2 | 04-08-2011 03:16 PM |


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AUSTIN -- A move to legalize switchblade knives is nearing success in Texas.
In a unanimous vote on Friday, the Senate Criminal Justice Committee advanced the bill. It has already cleared the House.
The automatic knives were outlawed across the country as an anti-gang measure in the 1950s. Several states have legalized them in the last few years at the urging of collectors and outdoorsmen.
The measure's author, Houston Democrat Harold Dutton, had originally said he wanted to use the knife bill to open a debate on weapons laws, including gun control. But conservative Republicans embraced the measure, and it has produced few opportunities for debate.
After a quick vote as his desk, Committee Chairman John Whitmire shrugged. He said, "I figure everybody's got a switchblade if they want one anyway."


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Hawaiian Lifeguard Association
From Openwaterpedia
The Hawaiian Lifeguard Association is a professional association incorporated in the State of Hawaii, with United States Internal Revenue Service 501(c) status. The association was organized in 1990, as an offshoot of the former Hawaii Lifesaving Association, a local chapter of the United States Lifesaving Association. The re-organization was reformed as a matter of refocusing the association to deal with specific educational issues related to providing lifeguarding and ocean safety services in Hawaii's unique culture and ocean environment.Mission
The Hawaiian Lifeguard Association seeks to:
- establish and maintain the highest standards of professional surf and open water lifesaving in an effort to maximize the safety of the public in ocean and coastal waters throughout the State of Hawaii.
- provide public, professional, and governmental education in the field of ocean safety, particularly in the areas of rescue management, emergency medical care and treatment, and to conduct informational and educational activities designed to prevent aquatic emergencies in Hawaiian waters.
- actively support and promote the improvement and progression of lifesaving throughout the State of Hawaii.


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Web Oriented Resources Management - Summary
Group identification
Id: #5244
System Name: worm
Name: Web Oriented Resources Management
Group Type: non-GNU software and documentation
Search in this Group
This project is not part of the GNU Project.
The pu rpose of WORM is to help the generation of Web pages in a professional, educational or administrative environment. Based on XHTML/CSS, using PHP and XML, WORM is a cross platform project
Registration Date: Fri 12 Sep 2003 07:00:56 PM UTC
License: GNU Lesser General Public License
Development Status: 1 - Planning
Communication Tools
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This project is looking for people (1 contributor wanted)
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Fostering Hope Inc. is a nonprofit organization that provides goods and services to children residing in the foster care system in Horry, Georgetown, Marion, and Dillon counties in north eastern South Carolina. There are on average over 500 children within these counties that are in foster care due to abuse and or neglect.
Fostering Hope 1001 2nd Ave Conway SC 29526 Phone 1(843) 254-8168


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Fabulous pick! Share your favorites and earn zulily credit. see detailsconnect
For gals on the go, this irresistible wallet does the trick! Styled in a lightweight straw material and covered in a modern print, it has a bevy of credit card slots and pockets, making this smaller shape a perfect pick for a night out.


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Quality CareFind out why Mayo Clinic is the right place for your health care. Make an appointment.
Meet the StaffFind a directory of doctors and departments at all Mayo Clinic campuses. Visit now.
Research and Clinical TrialsSee how Mayo Clinic research and clinical trials advance the science of medicine and improve patient care. Explore now.
Visit Our SchoolsEducators at Mayo Clinic train tomorrow's leaders to deliver compassionate, high-value, safe patient care. Choose a degree.
Professional ServicesExplore Mayo Clinic's many resources and see jobs available for medical professionals. Get updates.
Give to Mayo ClinicYou are an essential partner in our work to find answers for our patients. Donate now.
Prenatal care is an important part of a healthy pregnancy, starting as soon as you think you're pregnant. As your pregnancy progresses, you'll continue to visit your health care provider regularly — probably once a month throughout the second trimester, or weeks 14 to 27 of pregnancy.
Here's what to expect at your second trimester prenatal appointments.
Your health care provider will check your blood pressure and weight at every visit. Mention any signs or symptoms you've been experiencing.
Then it's time for your baby to take center stage. Your health care provider might:
You might need blood tests to check for low iron levels or gestational diabetes, a type of diabetes that can develop during pregnancy.
If you have Rh negative blood — an inherited trait that refers to a specific protein found on the surface of red blood cells — you might need a blood test to check for Rh antibodies. These antibodies can develop if your baby has Rh positive blood and your Rh negative blood mixes with your baby's blood. Without treatment, the antibodies could cross the placenta and attack the baby's red blood cells — particularly in a subsequent pregnancy with an Rh positive baby.
A urine sample might be tested for signs of a bladder, urinary tract or kidney infection.
Mayo Clinic is a not-for-profit organization. Proceeds from website advertising help support our mission. Mayo Clinic does not endorse non-Mayo products and services.
Advertising & Sponsorship
Check out these best-sellers and special offers on books and newsletters from Mayo Clinic.


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The Washington Monument Grounds
Posted 10 November 2010 - 09:53 AM
You just crack me up!!!!!!!!!
I can hear you talking (almost literally) in the way you write/explain things; all that exuberance ... lol.... tooooooooo funny!!!
Great story; as can only be told by you.
Glad it worked out.
*** Any day flying is a good day; have a great one!
**** REVS: Fly it, you'll like it!
***** L.S.P. ... It's worth the trip!
Posted 10 November 2010 - 10:03 AM
After the last phone call the Sargent on duty said he was "expecting our call and we are welcome back on the grounds",
A. if we don't use Abrasive Coated Flying line,
B. if we don't leave residual bits of kiting materials on the grounds,
C. if we place no stakes in the ground or tie-off on the trees,
D. if we don't interfere with the other spectators.
We may go after a permit anyway, if we can work out the details w/o a significant cost to the club.
Geesh what an exciting day!
I like B & D!!!!!!!!!!
D for obvious reasons and B we just won't discuss.................!!!!!!!!!!!!<WINK WINK>
"Cya in the Sand!....."
"Slack lines are fine lines!"
"You don't need a weatherman to know which way the wind blows" BD
"One good thing about music, when it hits you feel no pain" BM
Posted 17 November 2010 - 09:10 AM
Rev Themed Ts & Goodies: http://www.zazzle.com/LeshockArts
Aerostakes /Art & Design: http://www.leshockarts.com/
Rev Galleries: http://www.revkites....=user&user=1408
Flickr Pics: http://www.flickr.co...s/16896184@N05/
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for Employment Opportunities
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Rodriguez leads Hidalgo County District Attorney's raceWhen early voting results were released after polls closed at 7 p.m., Rodriguez had a lead in the race...04 March 2014Read More...
Local Weather
Current Conditions:
Fair, 69 F
Tue - Clear. High: 85 Low: 59
Wed - Partly Cloudy/Wind. High: 69 Low: 46
Thu - Mostly Sunny. High: 68 Low: 52
Fri - Partly Cloudy. High: 78 Low: 60
Sat - Partly Cloudy. High: 79 Low: 61
Full Forecast at Yahoo! Weather
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SAN ANTONIO – Though the Palmview Lobos took home the District 31 Wrestling Championships a week ago, their showing at the Region IV tournament was not as pretty. However, Christian Garza did finish third and will be moving on to the state tournament in two weeks.
Garza won his first two matches, taking down Senon Moya of Weslaco East and Rudy Rivera of Macarthur. After losing to Will Becker of Cedar Park in the third round, Garza rebounded by taking down Nikki Rowe's Akim Gonzalez and then Frankie Garcia of Georgetown for the third place finish.
On the girls' side, La Joya and Juarez benefitted from strong showings. Danae Sanchez of Juarez-Lincoln and Stephanie Ramirez of La Joya each placed second in their respective weight categories. Sanchez competed in the 148 weight class, losing to Lisa Gonzalez of Rio Grande City for the title. Ramirez was in the 185 weight class and lost in the championship to Dominique Turner of Churchill.
There were other boy and girl athletes who had strong showings as well. Mission's Mario Gomez made it to the final for fifth place before losing to Austin Boles of Austin Akins. On the girls' side, Mallaly Aleman of Juarez finished fifth by beating Brenda Cardenas of Nikki Rowe. Also, Sandra Munoz took fourth after losing to Christine Chessher in the 215 weight class.
For the actual brackets, click here for the boys and here for the girls.
< Prev | Next > |
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The Progress Times is the hometown newspaper for the local communities of Mission, Sharyland, Alton, Palmview, La Joya and surrounding areas in Western Hidalgo County. We have a staff of veteran reporters who work diligently every week to bring our readers the latest news as it affects their hometown area and people.
© Copyright 2012 Progress Times, All Rights Reserved


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Reviewed by 1 customer
Displaying review 1
- Cute
- Stylish
- Too High For Me
Best Uses
- Casual Wear
- Date Night
- Going Out
Comments about Michael Antonio Women's Tudor Sandal:
Very nice, stylish, goes nice with jeans and slacks, but a little too high for me. Also narrow from the front.
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- Feels true to size
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- Feels too narrow
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Cedar Rapids Weather
Miss Iowa Takes Fifth Place Honors
LAS VEGAS, Nevada - Miss Iowa, Mariah Cary, was fourth runner up in Saturday night's Miss America Pageant. Mallory Hagan, Miss New York, took first place.
Cary is originally from Burlington but attends Mount Mercy College in Cedar Rapids for public relations. The 20-year-old was diagnosed at a young age with Tourette Syndrome and said if she won, she would have advocated for the disease. During the pageant, she performed tap for the talent portion of the night and answered a question about medical marijuana once she made the top five. She told the judges that she knew people who used marijuana for medical purposes, but she thought that should be the only reason people use it. Cary didn't walk away empty-handed though. For earning honors as Miss Iowa, she got over $50,000 in scholarships. Conversation GuidelinesBe KindDon't use abusive, offensive, threatening, racist, vulgar or sexually-oriented language. Share KnowledgeBe truthful. Share what you know and what you are passionate about. Stay focusedPromote lively and healthy debate. Stay on topic. Ask questions and give feedback on the story's topic. Report TroubleHelp us maintain a quality comment section by reporting comments that are offensive. If you see a comment that is offensive, or you feel violates our guidelines, simply click on the "x" to the far right of the comment to report it. read the full guidelines here » Commenting will be disabled on stories dealing with the following subject matter: Crime, sexual abuse, property fires, automobile accidents, Amber Alerts, Operation Quickfinds and suicides. |
What's On KCRG |


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This cordless, keyless combi drill set from Black & Decker includes an 18V Black & Decker combi drill, a 3 hour charger, two batteries and a carry case. The cordless drill itself has two speed settings, screwdriving function, and a 24-position clutch. A combi drill combines the power of a hammer drill with the convenience of a traditional rotary drill, giving you the best of both worlds.
*Free delivery over £50 refers to home delivery orders placed on diy.com only. Charges refer to the value of goods being delivered and excludes delivery from stores or services. Excludes bulk delivery.
B&Q accepts the above cards and also offers a range of in-store financial services. Learn more
© B&Q 2014 - Terms & Conditions


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Stories for February 22, 2011
Lawmaker Attempts To Set Limit On Tap Water Carcinogen
Democratic Assemblywoman Nora Campos has a bill that would require the state's Department of Public Health to set a standard for hexavalent chromium, also known as chromium six.
Airport Starts Construction On Terminal Expansion
Construction replaced destruction at Lindbergh Field today as workers began installing steel girders for the Terminal 2 expansion project.
Study Finds Americans Don't Get Enough Vitamin D
Only about 10 percent of Americans take in enough vitamin D each day to ward off breast cancer and other diseases, according to a study released Tuesday.
Trailer: 'Thor'
Suspected Drug Dealers Arraigned For Rental Truck Full Of Pot
Two suspected drug dealers, who were allegedly caught by authorities unloading hundreds of bales of marijuana into a rental moving truck outside an Imperial Beach home, were scheduled to be arraigned today in downtown San Diego.
NOVA scienceNOW: What's The Next Big Thing?
In this episode of NOVA scienceNOW, come face to face with social robots that understand human feelings, carry on conversations, even make jokes. Then travel to Haiti, where geologists investigate the 2010 earthquake not long after it struck for clues to how to better forecast future quakes. Afterwards, join engineers at General Motors who are testing tiny, two-wheeled cars called EN-Vs, which one day might drive themselves through city streets.
San Diego Home Prices Better Than Rest Of Nation
San Diego was one of two major housing markets that gained value in December when compared to prices a year ago.
These Days' Legal Update
This morning we discuss recent cases involving Zip Code information in credit card transactions; gay marriage standing and the religious employer exemption from Federal employment discrimination law.
Chula Vista's Illegitimate Tax
Could the defeat of Proposition H force Chula Vista to cut city services? We'll discuss the strange afterlife of the failed ballot measure.
Legislation To Ban Sale, Possession of Shark Fins In California
Legislation introduced in Sacramento would ensure that California ceases to be both a major supplier and consumer of shark fins through a ban on the possession, sale, trade, and distribution of fins. Hawaii has passed a similar ban. Oregon and Washington are also considering bans. Every year people kill up to 73 million sharks for shark fin soup, a practice considered wasteful and unsustainable. Some scientists say many shark populations have collapsed worldwide due to overfishing. We discuss the new legislation, the traditional Chinese shark fin soup and the status and importance of shark populations in the oceans.
Could U.S. Military Bring Order To Libya?
If the violence and unrest in Libya continues, should the U.S. or other members of the international community get involved? We speak to KPBS Guest Military Blogger Beth Ford Roth about the possibility that the U.S. could step in to bring order to the country.
Salk Scientists Might Cure Your Bald Spot
An effort to cure irritable bowel syndrome may have given us a drug that will cure baldness. There's already a move in the works to turn it into a marketable product.
App Enables Doctors To Access Medical Records On The Go
Palomar Pomerado Health has unveiled a prototype of a mobile wireless system designed to help doctors make better decisions. The application provides real-time patient information to smart phones and wireless tablets.
SD Councilmember Urges Stronger Stance On Water
Water is serious business in the City of San Diego. Still, the city doesn't have a comprehensive approach to handling its supply. But that could soon change.


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BAGHDAD (Reuters) – Iraq's capital wants the United States to apologize and pay $1 billion for the damage done to the city not by bombs but by blast walls and Humvees since the U.S.-led invasion that toppled Saddam Hussein. The city's government issued its demands in a statement on Wednesday…
By Katie Halper
Read MoreBy Gary Younge, The Guardian
Read MoreRaw Story is a progressive news site that focuses on stories often ignored in the mainstream media. While giving coverage to the big stories of the day, we also bring our readers' attention to policy, politics, legal and human rights stories that get ignored in an infotainment culture driven solely by pageviews.
Founded in 2004, Raw Story reaches 9 million unique readers per month and serves more than 30 million pageviews.
Copyright © 2004-2013 Raw Story Media, Inc. All rights reserved.


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Do you have a question? Chat with Us Live
All of our in-stock items ship the same day you order if your order is placed by 6 p.m. ET. Engrave & Ship today items will be shipped out the same day if personalization is approved by 3pm ET. Delivery to the 48 contiguous states takes 2-5 business days. Delivery to Hawaii, Alaska and Puerto Rico takes 5-7 business days. We will not deliver to a Post Office box.
We stand behind our products, period. If you need to return an unused stock product, we'll be happy to credit your account, exchange the item or give you a merchandise refund if returned within 30 days from receipt of your order.
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Simple yet great quality
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from Anchorage, AK
About Me Dept Manager, Education Industry
Best Uses
Comments about Baudville Character Pin - Bumblebee: Achieving the Impossible:
We use this product to honor our scholar recipients and their parents at our induction ceremonies. It's a great product because it's simple yet profound and we always try to present a positive message to our students and let them know we care and appreciate their accomplishment.
Product is what it said it would be.
By Renee
from Rio Vista, CA
Item will be just the thing for our event to present to those with a special thank you.
Want this item customized with your logo or organization's name?


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Could a world-class arts festival save the foundering America's Cup?
AMERICA'S CUP Clear your mind, if you can, of brawls over San Francisco piers and other obscenely expensive parcels of waterfront real estate. Focus solely on the inevitability of the 34th annual America's Cup.
Summer 2013, it'll rip into town, offering self-described "adrenaline sailing at its best" to jet-setting yachting enthusiasts. In 2010, the 33rd contest was won in Spanish waters by Oracle Racing, headed up by billionaire Larry Ellison. In 2013, Ellison plans to defend his trophy as the competition (ironically, dealing with its own financial struggles; the San Francisco Business Times reported March 23 that America's Cup officials laid off half their staff) makes its San Francisco Bay debut.
Of course, average San Franciscans — often found ransacking their couch cushions to scare up burrito funds — couldn't give a rat's ass about an event blatantly catering to the one percent. But they should, and here's why: unless we want to see all those Top-Siders stride directly to wine country after each day of racing concludes, we need to give the visitors (estimates vary on the numbers: 10,000? 200,000?) a reason to hang out in SF, visit its neighborhoods, and spend money locally.
One idea: organize an arts festival with programming complementary to the America's Cup races. Such an event would potentially offer a huge boost to the local arts scene.
The most passionate supporter of an America's Cup arts festival has got to be Andrew Wood, executive director of the San Francisco International Arts Festival. Last fall, he announced the 2013 SFIAF would shift its dates from May, when it usually takes place, to July through September. That way, SFIAF could coincide with the race — and be a component in what he envisions as a much larger, citywide event.
"We first contacted the America's Cup about including an arts component before they even confirmed San Francisco as the venue," Wood remembers. "They've never really had a strong arts component to the America's Cup before, but they've never tried to do anything like they're trying to do here."
He's referring to this particular race's unique appeal for "a land-based audience." Geographically speaking, some America's Cup races are viewable only to television audiences and anyone who happens to have a boat hanging out within sight of the course; the San Francisco Bay obviously offers far more viewing opportunities for landlubbers.
"If you do either of the two largest sporting events in the world — the Olympics and the World Cup — an arts festival is mandatory. You can't even bid on the Olympics unless you have a festival that's going to run alongside it," Wood explains. "[The event will then] appeal to more people. People will stay in the locale longer and spend more money — [especially important for] the America's Cup, where there's only racing for an hour a day."
Money is always a factor when planning for an arts festival of any size, particularly something large enough to entertain 200,000-ish people.
"We can raise a lot of our own money, but what we need is some type of agreement that says we can go out and raise it as the name 'America's Cup'," Wood says, noting that he's already broached the subject of fundraising with some of the consulates representing countries with boats entered in the race. He'd like to bring artists from all of the participating countries (so far: Italy, Spain, France, South Korea, New Zealand, China, and Sweden) to San Francisco to perform alongside Bay Area arts groups. His grand vision includes theme weeks for each country revolving around the various holidays that happen to fall within the race dates — for example, France's Bastille Day, July 14.
Ridiculous. You guys thought long and hard about how critical you all have been about the americas cup deal, and how you gleefully reported that it was downsized - and THIS is what you come up with?
a "world class" art festival?
Could you be more painfully small minded and provincial?
Why not a world class artisanal thimble collective? How about a world class review of edible mushroom varieties found in golden gate park between 30th ave and 32nd ave.
You guys are so completely lost these days... it is painful to watch.
Your straw-man arguments aren't.
Neither is the America's Cup, when you really read the reports.
I can't believe you gave Andrew Wood a megaphone. That guy is a crook and a con artist. He owes the City and many other people hundreds of thousands of dollars and has no intent to pay. He needs to go sink on one of those yachts!
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Battleship Cathy
Battleship Cathy is on a mission to find a romantic partner in a casual social setting!
4.54 / 5.00 20,825 ViewsThugjacker is the first game from Sokay.net. It's a beat 'em up along the lines of Double Dragon and Streets of rage. We've been working on this game for a million years and this is the first public release. This is a 2 level demo. Have fun.
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Despite concerns about possible funding cuts, the Pike Pioneers Nutrition Centers in Louisiana and Bowling Green continue to serve more than 200 seniors and disabled people four days a week.
"We're still plugging along," said center coordinator Diane Redd last week in her Louisiana office.
Two years ago, the centers, funded through the Northeast Area Agency on Aging, suffered a funding cut from the Missouri legislature.
That caused the elimination of Thursday meals for those who get hot lunches at the centers and deliveries to those who are not able to get out of their homes.
"It's always tight," Redd said. "Their's no surplus of employees. When the cook is gone, I'm the cook."
For the full story, see the April 24 edition of the Louisiana Press-Journal.


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Cell Reports, Volume 3, Issue 1, 1-7, 06 December 2012
Accelerating the Tempo of the Segmentation Clock by Reducing the Number of Introns in the Hes7 Gene
Yukiko Harima1, 3, Yoshiki Takashima1, 5, Yuriko Ueda1, 2, Toshiyuki Ohtsuka1, 4 and Ryoichiro Kageyama1, 4, ,
1 Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan
2 Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan
3 Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan
4 Japan Science and Technology Agency, CREST, Shogoin-Kawahara, Sakyo-ku, Kyoto 606-8507, Japan
5 Current address: Medical and Biological Laboratories Co., LTD, Nagoya 460-0008, Japan
Graphical Abstract
► Reduction of Hes7 intron number accelerates the tempo of Hes7 oscillation ► Reduction of Hes7 intron number accelerates the tempo of somite segmentation ► Hes7 is a key segmentation pacemaker ► The number of introns may be important for normal timing of gene expression
- Periodic somite segmentation is controlled by the cyclic gene Hes7, whose oscillatory expression depends upon negative feedback with a delayed timing. The mechanism that regulates the pace of segmentation remains to be determined, but mathematical modeling has predicted that negative feedback with shorter delays would give rise to dampened but more rapid oscillations. Here, we show that reducing the number of introns within the Hes7 gene shortens the delay and results in a more rapid tempo of both Hes7 oscillation and somite segmentation, increasing the number of somites and vertebrae in the cervical and upper thoracic region. These results suggest that the number of introns is important for the appropriate tempo of oscillatory expression and that Hes7 is a key regulator of the pace of the segmentation clock.
During somitogenesis, Hes7 expression oscillates owing to delayed negative feedback in the presomitic mesoderm (PSM), and disruption of this oscillation blocks the oscillatory expression of many other genes, such as Lfng and Dusp4, leading to somite fusion (Pourquié, 2011; Oates et al., 2012; Eckalbar et al., 2012; Kageyama et al., 2012; Bessho et al., 2001; Niwa et al., 2007; Sparrow et al., 2012). This suggests that Hes7 plays an important role in the segmentation clock, although it remains to be determined whether Hes7 is the fundamental pacemaker or whether it acts downstream of another oscillator. The clock period can be altered by inhibition of Wnt signaling, inhibition of Notch signaling coupling, or mutations of Nrarp in mice or hes6 in zebrafish (Gibb et al., 2009; Herrgen et al., 2010; Schröter and Oates, 2010; Kim et al., 2011). However, the expression of β-catenin, a Wnt signaling effector, does not cycle (Aulehla et al., 2008), and Nrarp and hes6 are not essential for periodic segmentation (Wright et al., 2009; Schröter and Oates, 2010; Kim et al., 2011). Thus, oscillators that are essential for segmentation are not included in the list of genes that could affect the pace of segmentation. In addition, all mutations that have been reported to date result solely in disruption or slower tempos of the segmentation clock (Oates et al., 2012; Kageyama et al., 2012; Bessho et al., 2001; Schröter and Oates, 2010; Kim et al., 2011), and such defects are also observed under other conditions, such as lower temperatures, that slow or inhibit embryogenesis in general (Jiang et al., 2000).
Mathematical modeling suggests that negative feedback with appropriate transcriptional/translational delays, which include the time required for transcription, splicing, translation, and transport of messenger RNA (mRNA) and protein products, underlies the oscillatory expression of the segmentation clock genes (Lewis, 2003; Monk, 2003; Jensen et al., 2003; Hirata et al., 2004; Zeiser et al., 2008). The sum of such delays regulates the stability and period of the oscillation, and shorter delays would both accelerate the tempo of the oscillation and dampen or abolish it (Lewis, 2003; Monk, 2003; Jensen et al., 2003). If Hes7 is the fundamental pacemaker, manipulations that increase the frequency of Hes7 oscillation should lead to a faster tempo (i.e., a shorter period) of somite segmentation. One way to shorten the delays and accelerate Hes7 oscillation is to delete the introns, because transcription and splicing of intron sequences increase the time necessary for mRNA production. The Hes7 gene has three introns, and deletion of all three introns reduces the delay by 19 min and completely abolishes oscillatory expression, leading to steady Hes7 expression and fusion of all somites (Takashima et al., 2011). Mathematical modeling suggests that such a short delay would abolish the oscillatory expression, and that a more moderate delay would give rise to more rapid but dampened oscillations (Takashima et al., 2011). We hypothesized that deletion of one or two introns from the Hes7 gene would lead to such a moderate delay, leading to shorter periodicity of somite segmentation, and proceeded to test that possibility.
Results and Discussion
Increasing the Number of Cervical and Upper Thoracic Vertebrae by Reducing the Number of Introns in the Hes7 Gene
Mathematical modeling suggests that negative feedback with an appropriate delayed timing is essential for sustained oscillatory expression, and that reduction of the delay by 19 min would abolish the oscillatory expression (Figure 1Ac). However, a more moderate delay (for example, 5 min shorter than the wild-type [WT]) would give rise to more rapid but dampened oscillations (Figure 1Ab, 8.9% shorter period than the WT shown in Figure 1Aa). We previously showed that deletion of all three introns within the Hes7 gene reduces the delay by 19 min and completely abolishes oscillatory expression (Takashima et al., 2011). Furthermore, we found that introduction of a Hes7 transgene lacking introns (pH7-Hes7-0; Figure S1) into WT mice caused severe segmentation defects in a dominant fashion even though two alleles of the WT Hes7 gene were present (Takashima et al., 2011). By contrast, introduction of the Hes7 transgene containing the three introns, which rescued the segmentation defects in Hes7 null mice, did not cause any defects in the WT background (pH7-Hes7-123; Figures S1, S2B, and S2C; Takashima et al., 2011). Taking advantage of this feature, we generated F0 transgenic mice carrying Hes7 transgenes lacking either one or two introns into the WT background and examined their vertebral segmentation. Hes7 transgenes containing two introns caused some minor segmentation defects, but most regions appeared largely normal (Figures S1 and S2D–S2F: first and second introns, pH7-Hes7-12, n = 2; first and third introns, pH7-Hes7-13, n = 2; second and third introns, pH7-Hes7-23, n = 2), suggesting that the segmentation proceeds almost normally when any two introns are present. However, introduction of Hes7 transgenes carrying a single intron showed more severe segmentation defects (Figures S1 and S2G–S2I: first intron alone, pH7-Hes7-1, n = 7; second intron, pH7-Hes7-2, n = 2; third intron, pH7-Hes7-3, n = 4). Mice carrying a Hes7 transgene containing either the first or third intron alone exhibited better segmentation than those carrying a transgene containing the second intron alone (Figures S2G–S2I). Interestingly, mice carrying a transgene containing the first or third intron alone had eight or nine cervical vertebrae (Figures S2G' and S2I'), whereas the WT and pH7-Hes7-123 mice had seven (Figures S2A'–S2C'). We performed subsequent experiments using the transgene containing the third intron alone (pH7-Hes7-3) because this transgene seemed to give rise to slightly better segmentations in the upper thoracic region.
We established two independent lines carrying pH7-Hes7-3 (Figure 1B) and measured Hes7 protein expression in the PSM. Line 1 expressed a high level of Hes7 protein in the PSM, whereas the level of expression by line 2 was similar to that of the WT endogenous level (Figure 1C). All line 1 mice (n = 11) exhibited eight or nine cervical vertebrae irrespective of the Hes7 background (+/+, +/−, or −/−), and line 2 (n = 4) exhibited seven (Figures 1Db, 1Dc, and S3). Furthermore, although the second thoracic (T2) vertebra in WT mice has a longer spinous process, this feature was instead associated with the T3 vertebra in some of the line 1 mice and all of the line 2 mice (Figures 1Dc, S3C, and S3D). These results indicate that one or two additional vertebrae formed in the cervical and upper thoracic region of the mutant compared with the control mice. When two additional vertebrae formed in line 1 of pH7-Hes7-3 mice, duplication of the first cervical vertebra (C1) and extra formation of C9 or T3 seemed to occur (Figures 1Db and 1Dc). Before cervical somites form, four pairs of occipital somites form. Furthermore, it has been reported that Her/Hes oscillation starts before the somite segmentation takes place in the chick (two cycles before somite formation) and zebrafish (five cycles before somite formation; Jouve et al., 2000; Riedel-Kruse et al., 2007). Thus, although it is unknown how many pulses of Hes7 oscillation occur before somite formation in mouse embryos, it is possible that at least six pulses of Hes7 oscillation occur before the first cervical somite forms. If this is the case, every six to eight somites, one extra somite could be formed in line 1. However, the vertebrae in the more-caudal region were fused in all of the pH7-Hes7-3 mice (Figure S2I). These results suggest that the segmentation clock is set at a faster tempo during the cervical and upper thoracic segmentations but is halted during the more-caudal segmentation in mice carrying pH7-Hes7-3.
We next compared the delay in gene expression from the genes lacking all introns, containing only the third intron, and containing all three introns. We used a ubiquitinated luciferase reporter under the control of the Hes1 promoter as previously described (Takashima et al., 2011). This analysis showed that the gene containing the third intron alone expressed the reporter protein ∼5 min earlier than the control gene (containing three introns) and ∼13 min later than the gene containing no intron (Figure S4), which was within the expected range of in vivo splicing kinetics (Audibert et al., 2002; Singh and Padgett, 2009). Mathematical modeling predicted that such a delay caused by a transgene containing the third intron alone would lead to faster but dampened oscillation (Figure 1Ab).
Increase of the Number and Decrease of the Size of Somites in the Anterior Region of pH7-Hes7-3 Mice
We compared the numbers of somites in pH7-Hes7-3 and control embryos at approximately embryonic day 8.5 (E8.5). At this stage, the control embryos contained 6.6 ± 0.5 somites, whereas their littermates carrying pH7-Hes7-3 had 7.4 ± 1.3 somites, indicating that the latter contained on average ∼0.8 more somites than the control at this stage. However, the somite number varied between embryos even in WT mice (commonly differing by three or four somites; Tam, 1981), and thus it was difficult to determine whether the observed difference was due to normal variability or a faster tempo of the segmentation clock. We therefore counted the number of somites that formed in the anterior region at E10.5 by using the forelimb as a landmark. In situ hybridization of Uncx4.1 revealed that there were 10.1 ± 0.1 Uncx4.1-expressing somites anterior to the posterior border of the forelimb in the control (Figures 2Aa and 2A'), whereas there were 11.4 ± 0.1 Uncx4.1-expressing somites in the same region of pH7-Hes7-3 embryos (Figures 2Ab, 2Ac, and 2A'). These results indicate that on average 1.3 additional somites form in the anterior region of the mutant. In agreement with the vertebral fusion defects in the caudal region, somites were fused caudally to the forelimb in the mutant (Figures 2Ab and 2Ac). These data suggest that the segmentation clock is set at a faster tempo but is subsequently halted during the formation of more-caudal somites.
We next examined the anterior border of Hox expression. The relative positions of the anterior borders of Hoxb6 and Hoxb9 expression to the forelimb bud were very similar between the control and pH7-Hes7-3 embryos (Figures 2B and 2C, arrowheads). However, whereas the anterior borders of Hoxb6 and Hoxb9 expression correspond to the 10th and 13th somites, respectively, in control mice (Alexander et al., 2009), they corresponded on average to the 10.9th and 14.7th somites, respectively, in the mutant (Figures 2B, 2B', 2C, and 2C'). These results suggest that the anterior border of Hoxb6 and Hoxb9 expression was shifted caudally by one or two additional somites in pH7-Hes7-3 embryos, supporting the idea of a faster tempo of the segmentation clock. This result also indicates that the link between the segmentation clock and Hox gene activation (Zákány et al., 2001) can be easily dissociated, as observed in zebrafish hes6 mutation (Schröter and Oates, 2010).
If the segmentation clock were set at a greater frequency, the somite size would be smaller, in contrast to the larger somites observed when the clock cycles at a slower frequency (Schröter and Oates, 2010). Therefore, we compared the lengths of the three most recently formed somites between control and pH7-Hes7-3 embryos at the same stages. We found that they were reduced by 10%–20% in pH7-Hes7-3 embryos compared with the control at various stages (Figure 3). These data suggest that the tempo of somite segmentation is increased in mice carrying pH7-Hes7-3.
Accelerated Tempo of the Segmentation Clock of pH7-Hes7-3 Mice
To show decisively that the tempo of Hes7 oscillation and somite segmentation is accelerated in pH7-Hes7-3 embryos, we performed time-lapse imaging of whole-embryo cultures at E8.5. We examined Hes7 oscillations by using the Hes7 reporter pHes7-UbLuc (Takashima et al., 2011; Niwa et al., 2011). In control embryos, Hes7 expression oscillated with an average period of 126.6 ± 2.0 min (Figures 4A, 4C, and 4D; Movie S1. Hes7 Oscillation and Somite Segmentation in a WT Mouse at E8.5, Related to Figure 4). Furthermore, somite segmentation occurred with a similar periodicity (Figure 4A; Movie S1. Hes7 Oscillation and Somite Segmentation in a WT Mouse at E8.5, Related to Figure 4). In contrast, in pH7-Hes7-3 embryos, Hes7 expression oscillated with an average period of 115.4 ± 1.1 min (8.8% shorter than the control), and somite segmentation also occurred with the same periodicity (Figures 4B–4D; Movie S2. Hes7 Oscillation and Somite Segmentation in a pH7-Hes7-3 Mouse at E8.5, Related to Figure 4). These data indicate that removal of the two introns leads to a more rapid tempo of Hes7 oscillation and somite segmentation.
It has been mathematically predicted that altering the delay in negative feedback of clock genes should alter the oscillation period. Indeed, altering the intron length of an artificial oscillator gene results in alteration of the period (Swinburne et al., 2008). However, Stauber et al., 2012 recently reported that elongation of the intron length of another oscillator gene, Lfng, had no effect on the segmentation period. Thus, they were not able to alter the period by changing the delays in negative feedback of the natural clock genes. This could be because such an elongation was insufficient to cause a significantly longer delay. Alternatively, Lfng may not be a pace-making clock gene, because its oscillation is regulated by Hes7 (Bessho et al., 2001). Stauber et al., 2012 also attempted to elongate the intron length of the essential oscillator gene Hes7, but this abolished its expression, and therefore they were not able to change the period. Here, we found that reducing the number of introns in the Hes7 gene led to a faster tempo of the segmentation clock, which suggests that Hes7 is a pace-making clock gene for somite segmentation. Our study also shows that the number of introns is very important for the period and stability of oscillatory expression. Complete lack of introns abolishes oscillatory expression (Takashima et al., 2011), whereas removal of two introns leads to a more frequent but dampened oscillation. In contrast, the presence of two introns led to mostly normal segmentation, although there were some minor defects in caudal regions. Interestingly, another mouse oscillator gene, Hes5, has two introns (Takebayashi et al., 1995; Dunwoodie et al., 2002). Furthermore, the essential zebrafish oscillator genes her1 and her7 contain three and two introns, respectively (Gajewski et al., 2003). These data suggest that at least two introns are required for oscillatory expression of the segmentation clock genes, and point to the significant role of introns in the timing of gene expression.
Experimental Procedures
Mathematical Simulation
Hes7 oscillations were simulated with the following equations as previously described (Hirata et al., 2004):
Because transcription is inhibited by a dimer of Hes7 protein, we assume
Transgenic Mice
The Hes7 transgene with three introns (pH7-Hes7-123) consisted of the genomic fragment of the Hes7 promoter region (5,393 bp upstream fragment from the first codon), a hemagglutinin (HA) tag fragment at the amino terminus, and a genomic sequence from the second codon to 76 bp downstream of the putative polyadenylation signal. To remove one or two introns, the intron-exon regions were replaced with Hes7 cDNA fragments. For the intronless Hes7 transgene (pH7-Hes7-0), the whole Hes7 coding and intron regions were replaced with Hes7 cDNA. Transgenic mice were generated by injecting linearized constructs without any vector sequence into the pronucleus of fertilized eggs. Mice carrying the Hes7 reporter pH7-UbLuc-In(−) were previously described (Takashima et al., 2011).
Genotyping was performed by PCR using the following primers:
WT mice: 5′-AGAAAGGGCAGGGAGAAGTGGGCGAGCCAC-3′ and 5′-GTTCTGAGAGCGAGAGGGGGTCTGGGATGG-3′
Hes7 null mice: 5′-AGAAAGGGCAGGGAGAAGTGGGCGAGCCAC-3′ and 5′-TTGGCTGCAGCCCGGGGGATCCACTAGTTC-3′
pH7-Hes7-3 mice: 5′-CGTACCAGATTACGCTATGGTC-3′, 5′-CCCAAGCCTGCTCCTTG-3′ and 5′-ACGGCGAACTCTAATATCTCCGCTTTCTC-3′
pH7-UbLuc-In(−) reporter mice: 5′-TACTGGTCTGCCTAAAGGTG-3′ and 5′-CCACCAGAAGCAATTTCGTG-3′
Western Blotting
The PSM parts of four embryos were mixed with 15 μl of lysis buffer (50 mM Tris-HCl pH 8.0, 100 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1× proteinase inhibitor cocktail, 1 mM phenylmethanesulfonylfluoride, 250 U/ml Benzonase) and incubated on ice for 30 min. After addition of 1.5 μl of 10% SDS, the samples were boiled and the protein concentrations were measured. The protein solution was boiled in sample buffer and then run on 12.5% SDS-PAGE. After the protein was transferred from the gel to polyvinylidene fluoride membrane (Millipore), the membrane was immersed in buffer containing 5% skim milk, anti-Hes7 antibody (1/300; Bessho et al., 2003), and peroxidase-conjugated anti-guinea pig immunoglobulin G (IgG, 1/4,000; Chemicon) in sequence. Immunoreactive bands were visualized with ECL-plus (GE Healthcare) and LAS 3000mini (Fuji Film). The intensity of each band was calculated with Image Gauge (Fuji Film). After the antibodies were stripped, the membrane was immersed in buffer containing 5% skim milk, anti-β-tubulin IgG (Santa Cruz), and peroxidase-conjugated anti-rabbit IgG (GE Healthcare) in sequence. Immunoreactive bands were visualized with ECL (GE Healthcare).
Bone and Cartilage Staining
Bone and cartilage of neonates were stained with alizarin red and alcian blue, respectively, as described previously (Bessho et al., 2001).
Whole-Mount In Situ Hybridization
Whole-mount in situ hybridization was performed as described previously (Bessho et al., 2001). For Hoxb6 and Hoxb9 probes, we amplified the genomic fragments by PCR using the following primers: 5′-TACCAGACCCTGGAGCTGGAG-3′ and 5′-ACCGAAGTATTTCACGTCCGG-3′ (Hoxb6), and 5′-TACCAGACCCTGGAGCTGGAG-3′ and 5′-CTAGTGGATCCTCAGGGCTCC-3′ (Hoxb9).
Bioluminescence Imaging of the PSM
All images were recorded in 16 bit with Image-Pro Plus (Media Cybernetics) and other equipment as described previously (Masamizu et al., 2006) with the following modifications: a 10× UPlan FLN objective lens (NA 0.30) was used with 4×4 binning and exposure time of 4 min 30 s. Images were analyzed with ImageJ software.
We thank H. Miyachi for help in generating the transgenic mice, and H. Shimojo, A. Isomura, and Y. Niwa for technical help and discussion. This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Supplemental Information
- Movie S1. Hes7 Oscillation and Somite Segmentation in a WT Mouse at E8.5, Related to Figure 4 (AVI 1555 kb)
- Arrowheads indicate newly formed somite boundaries.
- Movie S2. Hes7 Oscillation and Somite Segmentation in a pH7-Hes7-3 Mouse at E8.5, Related to Figure 4 (AVI 1758 kb)
- Arrowheads indicate newly formed somite boundaries.
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Oates et al., 2012 (2012). Patterning embryos with oscillations: structure, function and dynamics of the vertebrate segmentation clock. Development 139, 625–639. CrossRef | PubMed
Pourquié, 2011 (2011). Vertebrate segmentation: from cyclic gene networks to scoliosis. Cell 145, 650–663. Abstract | Full Text | PDF (1326 kb) | CrossRef | PubMed
Niwa et al., 2007 (2007). The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock. Dev. Cell 13, 298–304. Abstract | Full Text | PDF (1010 kb) | CrossRef | PubMed
Niwa et al., 2011 (2011). Different types of oscillations in Notch and Fgf signaling regulate the spatiotemporal periodicity of somitogenesis. Genes Dev. 25, 1115–1120. CrossRef | PubMed
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Publication Information
Received: September 14, 2012
Revised: October 26, 2012
Accepted: November 15, 2012
Published: December 6, 2012


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Scripture gets put to shoe leather on a continual basis at HOPE in Lancaster.
Members of the local Omega Psi Phi alumni chapter know it's better to give than to receive but recently, they did both.
A car fire near the top of a Lancaster hill on Christmas Eve didn't end well for the shuttered business at the bottom.
HEATH SPRINGS – Heath Springs officials will try to meet with Steve Gedney again next month to discuss their concerns with the town's sidewalk project.
A 30-year-old Kershaw man accidentally shot himself in the foot on Dec. 14, according to a Lancaster County Sheriff's Office incident report.
INDIAN LAND – Santa came early Christmas Eve for a few lucky children. Forget the sleigh and reindeer; he was riding shotgun on a firetruck.
Ava got her Christmas wish!
The South Carolina unemployment rate dropped in November and is the closest this state has been to the national rate since 2002, according to a release issued by the S.C. Department of Employment and Workforce on Friday, Dec. 20.
County officials are only one ordinance reading away from establishing the updated structure for the county's 911 system.
A Lancaster man and his girlfriend were not harmed after someone fired a bullet into their bedroom earlier this month.


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Raxis Prime: Hidden X-Wing Cheat for Star Wars The Force Unleashed: Ultimate Sith Edition
Home / Xbox 360 / All Cheats /This page contains Star Wars The Force Unleashed: Ultimate Sith Edition Hints for Xbox 360 called "Raxis Prime: Hidden X-Wing" and has been posted or updated on Sep 17, 2008 by Waicyacutty. Let us know what you think of this Hint by thumbs voting.
- Developer: LucasArts
- Publisher: LucasArts
- Genre: Action General
- Release Date: Nov 3, 2009
- ESRB: Teen
Raxis Prime: Hidden X-Wing
After you have electric bombed the initial entrance to the Hyperdrive Core three times, stand facing away from the entrance and walk to the right slightly. Look into the toxic waster sludge and you will automatically target something. Raise it up with Force Grip to see that it is an X-Wing.
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StephenKing.com Message Board
Written Works
> when does sheemies tale come out?
View Full Version :
when does sheemies tale come out?
January 13th, 2011, 03:31 PM
cause its not dec 29th
January 13th, 2011, 03:48 PM
That was the date we were given but according to Marvel's web site (http://marvel.wikia.com/Dark_Tower:_Sheemie%27s_Tale_Vol_1_1), the date is now January 26, 2011.
January 13th, 2011, 04:36 PM
Is there a good site for seeing what has been released for the Dark Tower in the collected hard cover edition? I enjoy reading them but am not nor will be a monthly collector of the series. I prefer to wait until they are all out in one edition and then getting that.
The last one I read was the fall of Gilead and I think I saw people mention that the Battle of Jericho Hill series was going on not that long ago.
Has that been released in hard cover?
January 13th, 2011, 04:56 PM
You can check our Tracker here (http://www.stephenking.com/promo/ipt/works/dark_tower_ipt.html).
January 13th, 2011, 06:44 PM
They have all been good quality reads, which is a tribute to the creative team and all their hard work-fleshing out scenarios and lending new dimensions to our fave alternate universe...
January 21st, 2011, 01:30 AM
everything has been released in HC form, except for the stand-hardcases. i understand your frustration and not wanting to pick them up monthly. BUT . . . there is some subject matter, specifically in the DT series, that is only in the monthly installments and make it worth your while.
January 24th, 2011, 10:37 PM
Is there a good site for seeing what has been released for the Dark Tower in the collected hard cover edition? I enjoy reading them but am not nor will be a monthly collector of the series. I prefer to wait until they are all out in one edition and then getting that.
The last one I read was the fall of Gilead and I think I saw people mention that the Battle of Jericho Hill series was going on not that long ago.
Has that been released in hard cover?
BOJH is out in hardcover, I have it. I also was never a comic book collector. I had never bought a comic before the Stand and Dark Tower stuff starting coming out. However there is extra stuff written by Robin Furth which is kind of interesting at the end of each DT comic book which is not reproduced in the hardcover compilations. I also was going to wait for the hardcovers until I realized they were not reproducing everything from the books in them. I really wish they would because it is a royal pain to keep up with comic book release dates. I don't know how comic book collectors deal with this, but it gives me a headache and I only buy the Stephen King stuff. Marvel used to be good with the dates but they changed their website and now it is hard to get accurate dates there. Lilja's Library stopped giving dates for them too. SK website generally tells you the date they are out lately.
I don't know what is going on with the Sheemie one shot but I am getting annoyed lol. The date has changed sooo many times. It is not on midtown comics weekly release list for this week and they are really accurate so who knows if it is indeed coming Wed. I like to buy the hardcovers when they come out too though so I can look back without damaging the books. Who knows, they may be worth something some day I can't imagine there are a lot of readers of this series out there unless they are a DT fan. Glad I found a fan base here.
January 31st, 2011, 10:36 PM
Has the release date of Sheemie's Tale been pushed back again? Also does anyone have a link to marvel (or another quality website) where you can subscribe to the monthly series? I've looked all over marvel's website and can subscribe to other comics but I have never really been into comics until the DT came out a few years back. .
February 1st, 2011, 01:58 AM
hmmmmm. any new updates?
February 1st, 2011, 10:19 AM
Marvel's web site says it came out last week. Not sure if this is true. I usually use this ecom site to verify dates.
http://www.tfaw.com/ (http://www.stephenking.com/forums/redirector.php?url=http%3A%2F%2Fwww.tfaw.com%2F)
Bev Vincent
February 1st, 2011, 10:29 AM
I can't imagine them releasing a standalone in the middle of an ongoing series. I'd be surprised if it came out before the end of the Little Sisters arc.
February 10th, 2011, 10:43 PM
I haven't seen any copies of Sheemie's Tale at the comic store either.........................
February 11th, 2011, 09:05 AM
Seriously, this is amateur behaviour. How can Marvel announce a comic-book and 4 months later it's still on hold???
March 31st, 2011, 01:26 AM
is this comic still on hold?? its driving me crazy i can find out an official answer..
Brother Rabbit
February 15th, 2012, 09:36 PM
when does sheemies tale come out?
For those of you who have come here searching for info on Sheemie's Tale. (as this is the first link that shows on Google)
Wikipedia now says:
"Sheemie's Tale, a one-shot that's meant to serve as an interlude between The Journey Begins and The Little Sisters of Eluria, was originally scheduled for publication in November 2010, then postponed to January 26, 2011, and has since been indefinitely put on hiatus"
Hope this helps people searching for this issue as it has taken me -over a year- to discover this reality and cross this issue off my want list.
Hope This Helps,
Brother Rabbit =:xB
February 16th, 2012, 09:39 AM
I'd say the odds are good that this issue never gets released at this point.
March 16th, 2012, 01:04 PM
Any updates? This is a weird situation.
March 16th, 2012, 02:03 PM
I've emailed Robin Furth to see if she has any information she can share with us.
October 18th, 2012, 03:00 AM
The new (final) issue of "The Man In Black" is out, and the inside back cover is an advert for the next issue: "Sheemie'sTale"! It doesn't say when it'll be out, though.
October 19th, 2012, 07:47 AM
...soon, very soon....
October 23rd, 2012, 08:15 AM
The monthly publication that solicits new comic releases (Diamond 'Previews') is out for November... it states that 'Sheemie's Tale' is now a two-issue story, and #1 is set to ship on Wednesday, 2 January, 2013. That's the way to kick off the new year...
October 23rd, 2012, 11:30 AM
(there is another sheemies tale out on the social groups)
January 10th, 2013, 04:38 AM
The first issue came out this week. It's pretty damn good, too.
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Geraldine J. "Jerry" Catuogno of San Antonio passed away at age 83 on Thursday, June 30, 2011 in Fredericksburg. She was born in 1927 in Mission to Elic and Marguerite Weatherford.
She is survived by her daughter, Marguerite E. Haley and husband C.C. She was known as "Nana" to her grandchildren Tara A. and Kyle P. Haley.
She was preceded in death by her husband of 49 years, Castanzo V. Catuogno, of Brooklyn, New York; brothers, James Thomas and Charles Weatherford of Mission; and son, Dane Michael Ardelean of Aurora, Illionois.
Visitation will be held at St. Matthew's Church, 10703 Wurzbach Rd., San Antonio, on Wednesday, July 6, 2011 from 5-7 p.m. with a 7:30 p.m. rosary. Funeral services will be held at St. Matthew's on Thursday, July 7 at 10 a.m. with interment at Ft. Sam Houston at 11:30 a.m.
To send flowers, or to make a memorial donation, the funeral home is Mission Park North, and the family's charity of choice is Hill Country Memorial Hospice, 808 Reuben St, Fredericksburg, TX 78624.
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Spotted Seatrout
Anglers, prepare your rods and reels; the recreational harvest season for spotted seatrout in southern Florida will reopen on Jan. 1. The opening rings in 2012, a recreational closure-free year that will include several spotted seatrout management changes effective Feb. 1.
Recreational spotted seatrout management changes include:
Spotted seatrout harvest has been closed in the southern region of the state since Nov. 1.
The southern region includes any Florida state waters and federal waters south of the Flagler-Volusia county line on the east coast and, in the Gulf, south of a line running due west from the westernmost point of Fred Howard Park Causeway, which is about 1.17 miles south of the Pinellas/Pasco county line.
The maximum daily bag limit for spotted seatrout in the southern region of the state is four fish per person. In northeast and northwest regions, the daily limit is five spotted seatrout per person until the new rules take effect Feb. 1.
The statewide slot limit for spotted seatrout is 15-20 inches total length, and anglers may keep one spotted seatrout larger than 20 inches as part of the daily bag limit.
Spotted seatrout may not be harvested by snagging or snatch-hooking or by any multiple hooks with live or dead natural bait. Spotted seatrout may be taken only with hook-and-line gear and cast nets, and must be landed in a whole condition.
FWC Facts:The largest lake in Florida is Lake Okeechobee.
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Antagonism between MES-4 and Polycomb Repressive Complex 2 Promotes Appropriate Gene Expression in C. elegans Germ Cells
Antagonism between MES-4 and Polycomb Repressive Complex 2 Promotes Appropriate Gene Expression in C. elegans Germ CellsRemoval of Polycomb Repressive Complex 2 Makes C. elegans Germ Cells Susceptible to Direct Conversion into Specific Somatic Cell Types
Cell Reports, Volume 2, Issue 5,
Pages 1169-1177,
Publication Date 25 October 2012
Copyright © 2012 The Authors All rights reserved.
DOI: 10.1016/j.celrep.2012.09.019
Laura J. Gaydos
Andreas Rechtsteiner
Thea A. Egelhofer
Coleen R. Carroll
Susan Strome
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA
Department of Biology, Indiana University, Bloomington, IN 47405, USA
Corresponding author
These authors contributed equally to this work
Present address: AIT Laboratories, Indianapolis, IN 46241, USA
► MES-2/3/6 and MES-4 cooperate to regulate gene expression in the germline ► MES-2/3/6 concentrates repressive H3K27me3 on somatic genes and the X chromosome ► MES-4 generates H3K36me3 on germline genes and repels repressive H3K27me3 ► Antagonistic histone modifiers shape chromatin landscapes and gene expression
The Caenorhabditis elegans MES proteins are key chromatin regulators of the germline. MES-2, MES-3, and MES-6 form the C. elegans Polycomb repressive complex 2 and generate repressive H3K27me3. MES-4 generates H3K36me3 on germline-expressed genes. Transcript profiling of dissected mutant germlines revealed that MES-2/3/6 and MES-4 cooperate to promote the expression of germline genes and repress the X chromosomes and somatic genes. Results from genome-wide chromatin immunoprecipitation showed that H3K27me3 and H3K36me3 occupy mutually exclusive domains on the autosomes and that H3K27me3 is enriched on the X. Loss of MES-4 from germline genes causes H3K27me3 to spread to germline genes, resulting in reduced H3K27me3 elsewhere on the autosomes and especially on the X. Our findings support a model in which H3K36me3 repels H3K27me3 from germline genes and concentrates it on other regions of the genome. This antagonism ensures proper patterns of gene expression for germ cells, which includes repression of somatic genes and the X chromosomes.


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Quick Facts
Best Known For
Joan Crawford was an Oscar-winning actress, dancer and executive. She was known for films like Whatever Happened to Baby Jane? and Mildred Pierce.
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Joan Crawford - Acting Career
Joan Crawford has become a Hollywood legend with an acting career that spans many memorable roles.
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Play NowJoan Crawford. (2014). The Biography Channel website. Retrieved 10:21, Mar 11, 2014, from http://www.biography.com/people/joan-crawford-9260899.
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Born on March 23, 1905, in San Antonio, Texas, Joan Crawford began dancing at a young age, and went on to act in dozens of films. She was one of Hollywood's top stars of the 1930s, earning an Oscar for her lead role in 1945's Mildred Pierce. She later became known for the horror classic Whatever Happened to Baby Jane? and was the subject of the memoir Mommie Dearest. She died on March 10, 1997, in New York City.
Early Life
Film actress Joan Crawford was born Lucille Fay LeSueur in San Antonio, Texas, on March 23, 1905 (though some sources have reported her birth date as 1908). Her parents separated before she was born, and her mother later married theater owner Harry Cassin. Crawford would come to be known as Billie Cassin growing up, and periodically throughout of her entertainment career.
After her mother and stepfather split, Crawford attended two private schools, where she worked on the premises to pay for tuition while also being treated harshly, receiving corporal punishment for perceived misdeeds. Because of her workload, she was unable to attend classes and her scholastic record was faked.
Big Break in 'Our Dancing Daughters'
After a short time at Stephens College, Crawford left to pursue a dancing career, a pastime to which she'd dedicated herself. She eventually danced in the Broadway show Innocent Eyes, and in 1925 started to work onscreen for MGM. She starred in a number of silent films during this period and was given the name "Joan Crawford" from a magazine contest sponsored by the studio. The actress hit it big with the smash Our Dancing Daughters (1928), in which she played a rich, lovelorn girl who loves to Charleston.
A prolific and long-lasting film career was to follow, with Crawford ultimately going on to star in more than five dozen films. She took on talking roles with projects like Hollywood Revue (1929) and Grand Hotel (1932), and her dancing skills were prominently displayed with Fred Astaire in the 1933 hit Dancing Lady. Clark Gable was also featured, and was a recurring co-star in works like Possessed (1931) and Strange Cargo (1940).
Oscar for 'Mildred Pierce'
Crawford was a major, top-earning star of the 1930s, though by the end of the decade, her pictures were meeting with limited success. She rallied again with A Woman's Face (1941) before leaving MGM and signing with Warner Brothers, eventually garnering the lead role in 1945's Mildred Pierce, about a mother who rises from humble beginnings to become a successful restaurateur. The film received several Academy Award nominations, and Crawford won for best actress.
Crawford would receive two more Oscar nominations over the years, one for her role as a schizophrenic nurse in another film by the name Possessed (1947), and the other as a playwright in the thriller Sudden Fear (1952), which she'd also produced. She became known for a steadfast devotion to her career and a willingness to adapt to different vehicles while cultivating a fan base.
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Thanks to our unending fascination with all things terrifying, Hollywood is constantly churning out horror films for our enjoyment. With no end of gruesome combinations of stalking, murder and mutilation, these films hinge on the terrorizing of innocent victims by a sick, disturbed murderer. In the midst of all the bloodshed, actresses in the role of scream queen provide a dose of eye candy—and a killer set of lungs. Some of these women have made their name in horror flicks, while others have played one frighteningly convincing role. These are the women who have played some of the iconic scream queens of the big screen.
Scream Queens 25 people in this group
Name Changers
In entertainment, where the line between fiction and reality is often blurry, names are a crucial part of a celebrity's image. Stage names are often chosen to make an actor or musician's name easier to pronounce or remember, or simply to make it sounds more attractive. Here are famous celebrities who have changed their names.
Name Changers 236 people in this group
Silent Screen Stars
In the early years of motion pictures, actors were recruited from the stage, resulting in larger-than-life performances that seemed jarring when blown up to the size of a movie screen. As the years went on, actors began to understand the subtleties of the medium, and used more natural expressions to connect with their audiences. They became movie stars, known for their glamorous looks and identifiable personalities. As Norma Desmond in Sunset Boulevard would say, they didn't need dialogue, they had faces.
Silent Screen Stars 16 people in this group


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1812 Soldiers' Field Day Fife & Drum Muster
Description: | Recognize the 1812 bicentennial at Fort George. This weekend-long event is a pageant of military music by Fife and Drum corps from the United States and Canada as well as a competition of 1812 drill teams from Fort George, Fort Malden, Fort York and Fort Erie a collection of the premier 1812 military sites in Ontario. During this 14th year of the annual Muster and Field Day event, the 41st Regiment Fife and Drum corps will also be celebrating their 25th anniversary at Fort George. Come and experience the sights and sounds of the military garrison during the bicentennial of the War of 1812. |
Location: | Fort George National Historic Site, Niagara-on-the-Lake |
URL: | http://www.pc.gc.ca/lhn-nhs/on/fortgeorge/ne/ne1.aspx |
Date: | Saturday, August 18, 2012 |
Repeat Type: | Daily;Count=2
Time: | 10:00am-4:00pm EDT |
Duration: | 6 hours |
Category: | General Events* |
Created by: | Manor Greystone |
Updated: | Saturday, March 24, 2012 5:27pm GMT |


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For Immediate Release – 6/25/2013Contact – Director of Communications, Rita Elliott 815-226-3374
Rockford, Ill., — Regional high school students are in for a real-life surprise when they are witness to an "accident" on campus. On Wednesday, June 26, 2013 beginning at 1:30 p.m., attendees of the Rural Health Careers Camp will witness an accident simulation in Rockford College's parking lot A. A realistic and real-time accident scene will be created and emergency crews from Rockford Police, Fire, and Ambulance will arrive on campus with emergency lights and sirens. Emergency crews will determine that "Flight for Life" will be necessary. Weather permitting; an emergency helicopter will then land on campus to treat the "victim" involved in the accident.
Rockford College is pleased to be the host site for the Rural Health Careers Camp. The three-day camp held June 26 – June 28, is intended for high school students residing in rural areas who have a budding interest in the health professions. The camp is a partnership between the National Center for Rural Health Professions (NCRHP) located at the University of Illinois Health Science Campus in Rockford, Rockford College and the Area Health Education Center (AHEC) of Northwest Illinois. 19 students are in attendance.
The purpose of the Rural Health Careers Camp is to provide rural high school students with a hands-on learning experience about different health professions careers, and to show them the opportunities available in health professions. Students will also receive information about education and training, academic requirements, financial aid, and campus life. The faculty for the camp is comprised of health professions faculty, health professionals currently in practice, and health professions college students. Current rural high school freshmen, sophomores, and juniors with a priority given to freshmen and sophomores from the 19 counties of the Northern Illinois Area Health Education Center (AHEC) Region are eligible to apply to the camp. Counties included are: Boone, Bureau, Carroll, DeKalb, Henderson, Henry, JoDaviess, Knox, LaSalle, Lee, Mercer, Ogle, Putnam, Rock Island, Stark, Stephenson, Warren, Whiteside and Winnebago.
Questions may also be directed to Vicki Weidenbacher-Hoper at firstname.lastname@example.com or call 815-395-5854.
3/6/2014 » 3/27/2014NICNE Certificate in Nonprofit Management - Course 4
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8 hours agoJeremy Long: Personal Allegories art exhibit to open March 21 at Rockford University
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2/27/2014Rockford University to celebrate the 167th year of its founding


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Back from a 8 sabbatical she is ready to share her life's experiences of the many obstacles we encounter in our lives that stop us from using our God given gifts and talent and from fulfilling our purpose and mission in our lives. Which keep us from enjoying our everyday lives with love peace and happiness.
Kim's mission is to relay the Az-U-R, "as you are", message that speaks of issues concerning psychological and social observances, drug and alcohol abuse, personal relationships, love, disappointment and self growth. The importance of acceptance and truth in being "As You Are" (Az-U-R). As He created you. That all things are possible through Jesus Christ.
With the soul of a rocker, her rich, soothing but powerful vocals that have a tonal quality that's unforgettable, with heart felt passion and enthusiasm. In hopes of touching all who will listen to the straight forward messages learned through her experiences. Intertwining her original songs from past and present, some of the rock classics favorites, contemporary Christian classic also including some the standard hymns. With positive uplifting truth and love, that we don't have to be victims, we can be victors.
For Booking Info: (270)994-5379
e-mail : firstname.lastname@example.com
Kim French Singer / Songwriter
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says ... on Monday, Oct 26 at 11:04 PM
You can do it and keep up the good work!
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says ... on Thursday, Aug 20 at 10:28 AM
Kim you got what it take keep on going all the way to the top and don't let no one stop you
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says ... on Sunday, Aug 16 at 4:53 PM
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says ... on Thursday, Aug 13 at 3:31 PM
Kim you are great , love your style , look for me on the youtube.com and tangle.com just type in gospelpicker I write and sing my own music, that was a beautiful song good job
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