text:
The Phi-TEC I is a computer-controlled adiabatic calorimeter that can be used to determine the heat evolved and pressure developed during an uncontrolled exothermic runaway reaction. It is the modern alternative to the classic ""ARC"" developed by Dow Chemicals.
The system is completely computer controlled and features a range of high-pressure test cells, magnetic stirring and pressure and temperature logging. Standard Pre-programmed Tests make runs quick and efficient and simple standard test cells keep consumable costs to a minimum.
Request info
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Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on March 30th 2014).


text:
from his twitter:
Drew Gooden: Pistons, Bucks, Cav's, Bobcats. Which team is the best fit for me?
he posted this about 2 hours ago. I just read all the NBA twitters on hoopshype. somebody needs to notify him that the Bobcats are a perfect fit for every NBA player.
I wouldn't mind having drew gooden as our backup big man. thoughts?

subset: commoncrawl


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Sculpt your way to the beach
Published on Wed, Feb 11, 2009 by
Matthew Nash
Read More
Local sculptors find inner peace from scraps.
Members of the Olympic Driftwood Sculptors meet once a month to scrape and sand pieces of wood into works of art.
"It is like a present nature gives you and you have to open it up," Tuttie Peetz, the certified instructor for the club, said about working on driftwood.
The group utilizes the "LuRon" method, which was adapted by Lucille Worland of Seattle. Worland spent time with members of the Makah Tribe in Neah Bay learning their process for crafting driftwood.
The "LuRon" process involves "eyeing" a piece of wood, looking deeper within. The artists rub the wood with a deer antler to compress the cells of the wood and bring out the shine.
They also make their own polish with beeswax and turpentine. Only natural products are used and bases for the art have to
complement it.
Members of the local club expanded on the "LuRon" method and formed "Beyond LuRon."
"This allows people to work outside of the box and try new things," Peetz said.
"Beyond LuRon" includes objects other than wood. One piece has a wine bottle held on its side. Peetz has a piece of art at home with dried kelp wrapped around it.
Projects range from a miniature, which can be a few inches, to extra-large. Some members are working on pieces more than 5 feet long.
Peetz prefers to call their driftwood "found wood" because most of their projects come from old clear cuts rather than a beach. In addition to a wood-gathering permit, she has pictures of finished sculptures in her car in case she needs proof when on her wood excursions.
Members range in experience from a few weeks to numerous years.
Bob Larsen, a member for three weeks, saw pictures of art in the Sequim Gazette and thought it would be therapeutic.
"It's definitely not the same thing when it goes from seaweed growing on it and all rotten to polished and done," said member Foster Thompson.
Some art takes only a few weeks to finish but others can take years.
"I leave a lot of stuff on it sometimes, because some of it is so fragile," said member Kay Kay.
"A piece might fall off and change the whole look and feel," member Barbara Ralph said.
Many members showed off their work with pride, asking if it looked like what they were calling it.
"The hardest part is picking a name," said Ginny Bullock, a new member.
"You never decide what it is going to be until you get far into it," Peetz said.
"We shouldn't try and give it a name because it limits you," Tony Ralph, said about the early process of sculpting.
The Olympic Driftwood
Sculptors formed in October 2008 and host 20 members at the Sequim Prairie Grange for their monthly meetings.
"We do it to share ideas and learn," club president Don Berger said.
"It shows me how wood flows and what to .... It also helps me get away from everything," Larsen said.
Olympic Driftwood
$15 a year to join
Required to take a class in six months to become a member
Meetings are 10 a.m.-2 p.m. the first Wednesday of every month at the Sequim Prairie Grange, 290 Macleay Road.
Prospective members are invited to attend a meeting to receive instruction from experienced club members. For more information, call 681-2535.
What: The Olympic Driftwood Sculptors' first art show
When: 10 a.m.-5 p.m. Feb. 21-22
Where: Dungeness River Audubon Center, 2151 W. Hendrickson Road, Sequim
Featuring: More than 100 pieces of art, art presentations and unfinished wood and wreaths of driftwood for sale.
Month of April: Display at Museum and Arts Center, 175 W. Cedar St.
Matthew Nash can be reached at mnash@sequim gazette.com.
The Sequim Gazette
is located at 147 W. Washington Street in Sequim.
Business hours are Monday through Friday from 8:30 a.m. to 5:00 p.m.
, or toll free at 800-829-5810. FAX 360-683-6670.
For a complete company directory with contact information please click
Local display advertising
by PaperG
© 2009 Sequim Gazette. All rights reserved. 147 West Washington, Sequim, WA 98382 • 360.683.3311
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Forums → Newcomers Forum
Posted Oct 19, '08 at 6:55pm
3 posts
Hello I'm new.... This side is very beautifull. Very very good 10+++++
Posted Oct 19, '08 at 7:01pm
564 posts
Hiya there! Have fun :) And it is a very beautiful site, considering it's less than a year old!
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General CounselWelcome to the SUNY Office of General CounselThe State University of New York Office of General Counsel provides legal advice and guidance to the Board of Trustees, the Chancellor and employees of the SUNY System Administration, and the Presidents and employees of SUNY colleges. Mission StatementThe mission of Office of General Counsel is to provide comprehensive legal services to support one of the United States' leading state systems of higher education, the State University of New York. We protect and defend the University's legal interests in accordance with the laws of the United States and the State of New York and provide legal advice to the Board of Trustees, System Administration, the State University's campuses, and other components of SUNY. We track legal developments, issue legal guidance to campus professionals and provide training on understanding and complying with legal requirements. System and Campus Contact Information Staff Biographies for the Office of General Counsel Access PDF copies of the General Counsel Update. |


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Chad Livengood from the Detroit News and Michigan Radio's political analyst Jack Lessenberry discuss the growing awareness of Lansing's work groups, and how voters can know who or what is influencing these committees.
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text:
Bunionectomy Trial With GRT6005
This study has been completed.
Grünenthal GmbH
Information provided by:
Grünenthal GmbH
ClinicalTrials.gov Identifier:
First received: March 24, 2009
Last updated: June 21, 2010
Last verified: June 2010
- Full Text View
- Tabular View
- Study Results
- Disclaimer
- How to Read a Study Record
The purpose of the trial is to determine whether the new centrally acting analgesic is effective in comparison to placebo and an active comparator (morphine).
Condition | Intervention | Phase |
Post Operative Pain |
Drug: GRT6005 Drug: Morphine Drug: Placebo |
Phase 2 |
Study Type: | Interventional |
Study Design: | Allocation: Randomized Endpoint Classification: Efficacy Study Intervention Model: Parallel Assignment Masking: Double Blind (Subject, Caregiver, Investigator, Outcomes Assessor) Primary Purpose: Treatment |
Official Title: | A Randomized, Phase IIa Trial Evaluating the Safety and Efficacy of a New Centrally Acting Analgesic in Subjects With Moderate to Severe Pain Following Bunionectomy. |
Resource links provided by NLM:
Further study details as provided by Grünenthal GmbH:
Primary Outcome Measures:
- Sum of pain intensity 2-10 hours after Investigational medicinal product intake. Pain assessments [ Time Frame: 8 hours ] [ Designated as safety issue: No ]
Secondary Outcome Measures:
- Amount of rescue medication [ Time Frame: 24 hours ] [ Designated as safety issue: No ]
- Adverse events [ Time Frame: 24 hours ] [ Designated as safety issue: Yes ]Adverse Events reported by the participant as well as electrocardiogram, a measure of electrical activity of the heart and laboratory values
- Time to first rescue medication [ Time Frame: 24 hours ] [ Designated as safety issue: No ]
- Patient Global Impression of Change [ Time Frame: 24 hours ] [ Designated as safety issue: No ]
Enrollment: | 258 |
Study Start Date: | March 2009 |
Study Completion Date: | October 2009 |
Primary Completion Date: | August 2009 (Final data collection date for primary outcome measure) |
Arms | Assigned Interventions |
Experimental: A
Dose 1
Drug: GRT6005
liquid formulation, 200 to 600 µg, single dose, one day
Other Name: Morphine
Experimental: B
Dose 2
Drug: GRT6005
liquid formulation, 200 to 600 µg, single dose, one day
Other Name: Morphine
Experimental: C
Dose 3
Drug: GRT6005
liquid formulation, 200 to 600 µg, single dose, one day
Other Name: Morphine
Active Comparator: D
Drug: Morphine
60 mg
Placebo Comparator: E
Drug: Placebo
single dosage
Ages Eligible for Study: | 18 Years to 75 Years |
Genders Eligible for Study: | Both |
Accepts Healthy Volunteers: | No |
Inclusion Criteria:
- Subjects scheduled to undergo primary unilateral first metatarsal bunionectomy
Exclusion Criteria:
- Contraindications to, or history of allergy or hypersensitivity to morphine, fentanyl, hydrocodone, acetaminophen, heparin, polyethylene glycol 400 USP-NF or any compound planned to be used during the anesthesia, or their excipients.
- Concomitant inflammatory disease.
- Life-long history of seizure disorder or epilepsy.
- Subjects with impaired renal function
- Subjects with impaired hepatic function
- Female subjects who are pregnant or breastfeeding.
- Resting pulse rate is <50bpm or >100 bpm after 5 minutes rest in supine position
- resting blood pressure after 5 minutes rest in supine position: Systolic blood pressure is <100mmHg or >140 mmHg Diastolic blood pressure is <60 mmHg or > 90 mmHg
Contacts and Locations
More Information
No publications provided
Keywords provided by Grünenthal GmbH:
Additional relevant MeSH terms:
ClinicalTrials.gov processed this record on March 13, 2014
No publications provided
Responsible Party: | Dr. Stephen E. Daniels, Premiere Research |
ClinicalTrials.gov Identifier: | NCT00872885 History of Changes |
Other Study ID Numbers: | 425965 |
Study First Received: | March 24, 2009 |
Last Updated: | June 21, 2010 |
Health Authority: | United States: Food and Drug Administration |
Keywords provided by Grünenthal GmbH:
analgesic bunionectomy surgery post operative pain single Hallux valgus repair |
Additional relevant MeSH terms:
Pain, Postoperative Postoperative Complications Pathologic Processes Pain Signs and Symptoms Analgesics Morphine Sensory System Agents |
Peripheral Nervous System Agents Physiological Effects of Drugs Pharmacologic Actions Central Nervous System Agents Therapeutic Uses Analgesics, Opioid Central Nervous System Depressants Narcotics |
ClinicalTrials.gov processed this record on March 13, 2014


text:
Matthew T. Mason
Robotics Research and Advanced Applications, 1982.
Notes |
Text Reference |
Matthew T. Mason, "Adaptable Manipulation without Sensors," Robotics Research and Advanced Applications, 1982. |
BibTeX Reference |
@inproceedings{Mason_1982_1524, author = "Matthew T. Mason", title = "Adaptable Manipulation without Sensors", booktitle = "Robotics Research and Advanced Applications", year = "1982", } |
The Robotics Institute is part of the School of Computer Science, Carnegie Mellon University. Contact Us | Update Instructions |


text:
Potomac ferry service idea resurfaces
Thursday - 8/8/2013, 5:32am  ET
WOODBRIDGE, Va. - If you could, would you ditch the car and take a boat to work? A commuter ferry on the Potomac River only is being considered once again and this time there may be money to operate it.
The project was last seriously considered four years ago when some local leaders took the media on a ferry ride that started at Indian Head in Charles County, crossed the Potomac to the Occoquan River, and then made stops at Fort Belvoir and Alexandria before finishing at the Navy Yard in D.C.
But the concept never got off the ground because there was no money for it.
That has changed. Virginia Transportation Secretary Sean Connaughton says the state's new $4 billion transportation package may provide some of the the funds. And the project could be eligible for federal funding.
"That could include both money for the ferry itself as well as the construction of facilities to serve it," says Connaughton.
Providing docks for the ferry and parking at those docks was one of the major obstacles in 2009.
"Our opportunity may be here to see if we can find ourselves some small cheap boats, get this up and see how many people who can use it," says Connaughton.
The passenger-only ferry could be marketed not only to commuters, but also tourists.
The latest report on the ferry option is due in October. Connaughton says the passenger ferry could be back in the state's six-year transportation plan, which details which projects are slated for funding, starting next year.
Follow @WTOP on Twitter.
© 2013 WTOP. All Rights Reserved.


text:
Bee Movie Perfume
By Dreamworks for Women
Bee Movie Perfume by Dreamworks, This wonderful and fun fragrance for kids from dreamworks has an exciting blend of fern, bergamot, pineapple, lavender, juniper and geranium . All products are original, authentic name brands. We do not sell knockoffs or imitations.
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See all Dreamworks products
Customer Comments
Very nice and friendly site.. I will purchase again.. Thanks..
Bizrate.com Customer Survey


text:
Constipation-Common Sense advice for those who suffer from Constipation...
May 10th 2010 20:24
There are several reasons why you may feel constipated. And in order to fix this problem, you will need to rule out the rare cases and make sure that your constipation is caused by diet and lifestyle. For example, if you have diabetes, then chances are likely that you have experienced either diarrhea or constipation. If it isn't a condition such as diabetes or hypothyroidism, then fixing your constipation should be easy. You simply change your diet and lifestyle.
Diabetes and those with kidney & thyroid issues can be affected by constipation
Most diabetics suffer from constipation. The reason why is actually the same reason why kidney patients and those who have hypo or hyperthyroidism are likely to be constipated; Diabetics, with their blood sugar spikes actually damage the nerves in their intestinal walls. This causes the food to either pass too quickly causing diarrhea or too slowly causing constipation.
Prescription drugs could cause constipation
Because so many of the population relies on prescription drugs, constipation is a very common side effect. In a lot of drugs, diarrhea and constipation are two of the most common symptoms. The reason for this is how your body breaks down the drug...in a lot of cases, the nervous system is affected and your food either comes out too quickly or too slowly.
Constipation can also be caused by the foods you eat...
If you aren't diabetic, and don't have thyroid or kidney issues, then it is likely that constipation will all boil down to your diet. Vegetarians are hardly ever affected by constipation. The reason is that they get more than enough fiber in their diet. And if your everyday diet consists mainly of meats and potatoes, then your fiber intake could be seriously lacking. Studies have shown that just in the last 100 years, our fiber intake has decreased by nearly 50% The recommended fiber intake for an adult is 30 grams of fiber. But even this doesn't compare to what we ate 100 years ago . Double the fiber recommendation after you have gotten accustomed to eating more fiber.
For more on Fiber Rich Foods, click here
If you don't drink enough water, you may become constipated...
Even being slightly dehydrated can cause constipation. Water is needed to pass waste from your body. If you are dehydrated, the stools will stay in your body longer and come out harder. Drink half your weight in water (in ounces) daily. If you weigh 100 pounds, you should be getting 50 ounces of water a day.
Lack of exercise could cause constipation
Most Americans don't get anywhere near the amount of exercise they need. Waste passes through the body quicker when you are exercise because you are stimulating the body and forcing more blood throughout your body. Runners typically don't get constipated.
If you are suffering from constipation, the good news is that it is usually an easy fix. And provided that you aren't having an issue with it as a side effect and it is solely because of diet, then the good news is that it can be fixed. If you need constipation help, chances are good that it will all boil down to your diet.
Constipation means that a person has three or fewer bowel movements in a week. The stool can be hard and dry. Sometimes it is painful to pass. At one time or another, almost everyone gets constipated. In most cases, it lasts a short time and is not serious.
For more information, you can also check out Constipation Natural Remedies
98 |
Vote |


text:
Keyboard and mouse locker
Dogs, cats, small children, and computer keyboards: as they say on "Sesame Street," three of these things belong together. Obviously, PC keyboards are the odd man out in this group, but how can they all get along together in a world (your living room, for instance) in which three out of four respondents think "software" means plush toys? The answer may be as simple as KeyboardLock, a free tool from Booring that locks and unlocks your keyboard and mouse when you enter a password. Say you want to watch a movie on your laptop with pets … Read more


text:
Spinners. Smallest girl - Pearlie Turner, 408 East Long Ave. Been at it 3 years and runs six and seven sides. Her Sister (largest girl) runs only four sides. I found other cases where youngest sister did much more work than oldest and family stimulated her by praising her speed and the other's slowness. Location: Gastonia, North Carolina.
Click tabs to swap between content that is broken into logical sections.


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UNK Quick Links Quick Links A-Z Website Listing Office of the Chancellor Campus Maps UNK Factbook Calendar of Events Policies & Procedures Contact Information Registrar's Office UNK Alert Undergraduate Graduate International eCampus Scholarships & Fin. Aid Academic Affairs American Democracy Undergraduate Studies International Education Study Abroad Academic Calendar Colleges & Departments Computer Use Policy Course Catalog Library eCampus Classes Schedule of Classes Transcripts Firefly Academic Calendar Blackboard LoperMail eCampus Helpdesk (ITS) Major/Advisor Change (ugrad) Student Union Student Government LoperCAREERS Telecommunications The Antelope Online KLPR Radio UNK ID Card MyBLUE Blackboard Ektron Login Faculty Senate Firefly Future Catalog Helpdesk (ITS) Email Staff Senate Telephone Directory UNK ID Card myBlue Assessment Current Catalog Faculty Handbook Policies & Procedures The Antelope Online UNK Today UNK Factbook Business & Finance Academic & Career Services Contact Information Employment at UNK Human Resources ITS Police/Parking Services Dining Services Alumni Association NU Foundation NU System
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HAZARDOUS MATERIALS MANUAL AND CHEMICAL HYGIENE PLANPolicy for the safe use of hazardous materialsand the proper management of hazardous waste.(latest revision: October 2009)


text:
Tuesday, June 30, 2009
© Copyright 2014
Newton Citizen
COVINGTON - Five teenagers were wounded early Sunday morning when shots were fired at a large party in Trelawney subdivision.
Lt. Tyrone Oliver of the Newton County Sheriff's Office said that one or two men fired shots in the residence at 10 Trelawney Lane shortly after midnight Sunday, wounding five teenagers. Oliver said none of the teens' injuries were life-threatening. Four of the injured were taken to area hospitals for treatment and one was released on the scene to their guardian, according to a statement released by the Sheriff's Office. Oliver said the party was well-chaperoned and the suspect or suspects were at the residence without permission. "There were several hundred people there (at the party)," Oliver said. "We do have witnesses to the event, but being that it is an ongoing investigation, I can't say if there were one or two shooters." One of the teens who was injured in the shooting said Monday that a man showed up and started firing shots in the area of the home's garage. "All I know is that we were at the party and some dude showed up, and I'm guessing he was in a gang or something," the victim said. "The owner of the house made him leave, and he came back and he started shooting." Alice Queen can be reached at email@example.com like this story
- Shooting probe continues ( July 1, 2009 )
- Man dead in shooting
2 others injured Monday in neighborhood south of Covington ( June 30, 2009 ) - Man shot during argument ( April 20, 2010 )
- Police search for suspects in drive-by
House, truck damaged ( August 11, 2009 ) - Texas man brought back to Newton on murder charge ( August 24, 2011 )


text:
9477 profile views [ stats ]
More pictures...
registered February 11, 2006
Gender : Male
Birthday : December 28, 1991
Occupation : Student
Location : Lichfield, Midlands, United Kingdom
School : Birmingham City University (Birmingham, Midlands, United Kingdom)
Education : In college
Smoker : No
Drinker : Yes
All posts by Tanglewoodguit
All threads by Tanglewoodguit
Tanglewood: Not a clue :)
Behringer: v-tone
Behringer: some amp.
Hokada: Some acoustic
Tabs [ 1 ]
close [x]
Smoke yourself young, it's only a lung or two. I miss these snow-capped peaks, with their slopes and points of view. So hurry home lad, going backs to the places where maternal embraces are given. You're not on your own lad, you'll take things for granted and leave empty handed and sore. Ahhhhhhhhhhhhhhhhh Ahhhhhhhhhhhhhhhhh Bleed it out all over my paper. Leave it out, don't be scared of the wind. You'll soon find out that 3 years turns to vapour. It's a shame you can't breathe it back in. It's a shame you can't breathe it back in.
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By Food & Wine
The key to sealing in the flavor in this incredible brisket is to return the brisket to the cooker after turning off the heat and let the meat rest in the sauce for at least 15 minutes before serving. More Slow Cooker Recipes
© Fredrika StjärneSlow Cooker Spicy Brisket with Texas Caviar
3 tablespoons vegetable oil
2 1/2 pounds top brisket
2 tablespoons plus 1 teaspoon hot barbecue spice blend or Cajun spice blend
1 large onion, coarsely chopped
4 large garlic cloves, thinly sliced
3 cups chicken stock or low-sodium broth
One 28-ounce can whole tomatoes, chopped, juices reserved
1 1/2 cups black-eyed peas
1. In a very large skillet, heat 2 tablespoons of the oil. Sprinkle the brisket all over with 1 tablespoon plus 1 teaspoon of the spice blend. Add the brisket to the skillet and cook over moderately high heat until well-browned, about 5 minutes per side. Transfer the brisket to the slow cooker.
2. Add the remaining 1 tablespoon of oil to the skillet. Add the onion and garlic and cook over moderate heat, stirring occasionally, until softened, about 5 minutes. Add the remaining 1 tablespoon of the spice blend and cook, stirring, until fragrant, about 3 minutes. Add the stock and the tomatoes and their juices to the skillet and bring to a boil over high heat, scraping to release the browned bits on the bottom. Pour the mixture into the slow cooker. Cover and cook on high for 1 hour. Turn the heat to low and cook for 1 hour longer.
3. Add the black-eyed peas to the cooker, cover and cook on low for 3 1/2 hours, until the brisket and peas are tender.
4. Transfer the brisket to a carving board. Cover loosely with foil and rest for 15 minutes. Season the sauce with salt. Carve the brisket into 1/3 inch thick slices and using a large spatula, carefully return the brisket to the cooker. Turn off the cooker and let the brisket rest in the sauce for at least 15 minutes or up to 1 hour before serving.
Make Ahead The dish can be refrigerated for up to 3 days. Reheat gently.
More from Food & Wine
More Slow Cooker Recipes
Delicious Brisket Recipes
Fast Meat Recipes
Affordable Meat Recipes
Best Steak in the U.S.
Slow Cooker Spicy Brisket with Texas Caviar
By foodandwine.com | Shine Food – Tue, Jan 8, 2013 3:05 PM EST1 - 4 of 90
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Our Mission: Promote learning and personal development through education based programs, creative ideas and concepts, and continual professional development that prepare the Vanderbilt community for productive lifestyles.
Wellness is a positive, day-to-day approach to a long, healthful, active life. The Wellness Center is a hub for free educational resources, advocacy, and programs about hundreds of wellness-related topics.
What is Wellness all about? Explore our site to learn more.
Join our online community by visiting us on Facebook!
Follow us on Twitter @VandyWellness!!!
We welcome your suggestions or questions about our new site.
Please be patient while we update our website.


text:
Father and son stole iron to make tow bar (From Cotswold Journal)
Get involved! Send your photos, video, news & views by texting EJ NEWS to 80360 or e-mail us
Father and son stole iron to make tow bar
9:00pm Friday 30th November 2012 in News
A FATHER and son have admitted stealing a length of angle iron which they wanted to make into a tow bar.
Andrew Scarrott, aged 46, of Blackmore Park, Hanley Swan, near Upton-upon-Severn, and his son, also called Andrew Scarrott, 28, of Horbridge Avenue, Badsey, both admitted theft when they appeared before magistrates in Worcester on Tuesday.
The court was told how the men took the metal of unknown value from GL Hearn Holdings Ltd, Blackmore Park Industrial Estate, Malvern, at about 8.45am on November 13. Owen Beale, prosecuting, said: "The estate is derelict and disused and it's in the hands of receivers. They have suffered a great deal of thefts. At the time of this theft they had instructed a company to fence off the area to prevent further thefts."
Officers were called to the site and found the men, who had brought a van with them, carrying the metal beam and they were arrested.
Mark Sheward, defending, said: "It's essentially a site which has been used for fly-tipping for a number of years. Mr Scarrott senior wanted to build a tow bar for his van and needed some metal to do that. The site is open. It's not fenced off.
"They realise it was not their property to take even though it was on a site where people regularly abandon all sorts of property.
This is clearly not something they thought was an offence or they would have gone in the dead of night, not in the morning when the road was busy with passing traffic."
Both men were given a 12- month conditional discharge, ordered to pay £85 costs each and a £15 victim surcharge.
Comments are closed on this article.


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The Regional Economics division of the Richmond Fed's Research Department collects timely information about the Fifth District economy, tracks and analyzes regional data, and keeps the public informed on the latest economic developments.
Read the latest results of our monthly and quarterly surveys of Fifth District business activity.
View recent data and analysis on the Fifth District's economy produced by our Regional Economics division.
Listen to our regional economists provide updates on Fifth District and national economic conditions.
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The Regional Economics group invites you to participate in our Surveys of Business Activity and share information about your business and local economic conditions.
For more information, email us at email@example.com, or sign up by clicking on the link below.


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A Magnolia woman who brutally stabbed, beat and slit her son's throat was found guilty by a Montgomery County jury and sentenced to 40 years in prison Jan. 10.
Daphne Spurlock, 46, was convicted of Injury to a Child after a four-day trial in the 435th State District Court. She was on trial after an incident where police said she stabbed her son Michael repeatedly, slit his throat and stomped on his head and chest in their Magnolia home March 17, 2012. She told detectives that she was trying to drive demons out of her son. Her defense was insanity.
"I think the (Montgomery County District Attorney's) office did a great job in presenting the case to the jury," said Magnolia police detective Brian Clack, who was one of the first officers on scene. "They were able to convince the jury that she still knew right from wrong."
Montgomery County assistant district attorney Tyler Dunman said the case was difficult to prosecute because of the insanity plea.
Spurlock's attorney, George Parnham agreed and told the Montgomery County Police Reporter it was a hard case for both sides.
"An insanity defense is a very hard defense for a jury to understand, despite the severe presence of a mental illness," Parnham said. "To a large degree that's because of our statute in the state of Texas, which I have been fighting to change the past eight years so it is more in line with other states."
He added that many people who knew Spurlock noticed a change in her behavior weeks before the incident. She was even fired from her job at Walmart because of the changes.
As for Michael, the state awarded custody to his sister Michelle Arroyo. She said that he has went through several forms of rehabilitation and is now walking and talking.
"He is doing really good," Arroyo said. "It's been a challenge, but he's a fighter."
Clack and Magnolia Police Sgt. Lopez were the first to arrive on the scene and started emergency procedures until EMS workers arrived. Clack said that the incident and Michael are something he constantly thinks about.
"I think about it every day," he said. "I've never been involved with anything like this before."
Now that the trial is over, Clack said that he and other members of the Magnolia Police Department are looking forward to being able to see Michael again. They weren't allowed before, as Michael was a potential witness in the trial.
"He is a happy go-lucky kid," Clack said. "We had some stuff we were going to give him for Christmas that we can now give to him in person."


text:
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COOK beef in large, nonstick skillet over medium-high heat, stirring frequently, until no longer pink; drain. Stir in water, tomatoes with juice, chiles and chili powder. Cook until mixture comes to a boil. ADD pasta; return to a boil. Reduce heat to low; cover. Cook, stirring occasionally, for 14 to 16 minutes or until pasta is tender. STIR in evaporated milk. Remove from heat; stir in 1/4 cup cheese. Sprinkle additional cheese over top. Cover; let stand for 5 to 10 minutes before serving.
Kami Race from Hudson, NY
A hearty meal. This recipe was very easy to prepare, and the result was delicious. The ingredients I usually have on hand anyway!
Read More Reviews
STACEY SHEFFIELD from United States
Added black beans and hand full of corn for color
Stacey Sheffield from United States
Quick, easy and yummy! Will add to our rotation.
Christina Ward from Lakewood, OH
Not really hot at all like I thought it would be. My 1 year old LOVED it as well!!
Meg Ed from Cleveland
This has a great kick to it! We keep all the ingredients on hand its so easy and filling to eat! Everyone will ask for it again!
STEPHANIE ARHCER from DENVER
Jeannie from NJ
This is so easy and absolutely yummy! All three of my boys *loved* this recipe as well as my husband and in-laws. I did use a bit more shredded cheese because we love things cheesy, and I used half of a can of chiles instead of the whole can so it wouldn't be too spicy for the kids. Other than that the recipe is perfect just the way it is. This is now a permanent recipe in my recipe box!
SB from
I love a spicy mexican dish, and this easy one was no exception. I used a can of diced tomatoes with chillis, added black beans, corn,and added a little more taco seasoning. We love the 4 cheese mexican blend so I always use that, and added extra in the dish and on top. I only had a small amount left to reheat for lunch the next day, but it was awesome!
Sharon from Yosemite CA
Three families loved this recipe. It's easy and hardy. It was great as leftover lunch. Some of the kids ate 3 helpings. Next time I'm going to add some corn and black beans.
Wendy from Lena, IL
GREAT!! The whole family loved it!! This is one I'll make again and again!! There was no left overs!! I even shared this with my mother in-law. Wonderful!!!!!
Lorisa Smith from
This is a keeper! My family was very pleased. Works well for leftovers. It was a little too spicy for my kids so I may try a mild Rotel instead of the green chiles next time.
Lucinda Scherting from Bountiful, Ut
Perfect! Easy quick meal for my family. Will definitely make again.
Stephanee Eastman from Utah
I loved this dish! I never thought that homemade Mac and Cheese could be improved on, but this is it. This is my new favorite!
Janeen Jackson from Iowa
I love this recipe! It is so tasty. Highly recommended. (It was a little spicy for my kids though). I will probably leave the chiles out next time. Yum!
Gwen from
Wanting something different, this was definitely that. Substituted chiles and diced tomatoes for Rotel tomatoes and it added just more zip. Easy to prepare and very simple.
Shannon from State College, PA
This dish is incredibly easy to prepare and is also very tasty if you enjoy southwestern flavors. It was even better warmed up the next day.
Serving Size: 1/4 of recipe
Servings Per Recipe: 4
*Percent Daily Values are based on a 2,000 calorie diet.
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Dispatches From Israel
While working on my MSJ, I won a scholarship to do my journalism internship overseas. Through the kind support of the E.W. Scripps School of Journalism at Ohio University and Mrs. Shirley Fleischer, I interned at a news bureau in Jerusalem, Israel, during 2006. To see what mischief I got up to in the Holy Land, click on the links below. |
Dispatch Five: Is that a rocket in your pocket? (April 19, 2006) |
Dispatch Six: Layers of Time (May 2, 2006) |
What's a nice novelist like me doing in a place like this? |
Some people, both here and back home, have asked what on earth I'm doing on a journalism internship in Israel. Is this a career change? Did I quit fiction? Have I gone insane? No. No. And, not yet (but the night is young). |
So I decided to go get a master's degree in journalism, to expand my skills, opportunities, and résumé for writing fields besides fiction. I also wanted to earn a degree (the master's) that would potentially qualify me for some academic positions. | |
I attended the EW Scripps School of Journalism at Ohio University. And since experience is the best teacher, I applied for one of the overseas internship scholarships that's available via an endowment there. I chose to come to Israel because it's a very busy place, in terms of news, and I gathered from various advisors I consulted that I'd get more experience packed into an internship here than almost anywhere else. And after a few weeks on the job here, this seems to be true. |
Meanwhile, I have novels under contract and I'm working on them while I'm in Israel. As a quick tour of my website will reveal, I'm also still writing short fiction and essays for various editors with whom I have longtime associations. My fiction career continues at a healthy pace, and I look forward to its next few stages! |


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The Key Town Collection features a sophisticated finish and ornate details to create traditional designed furniture that is sure to enhance any home's decor. The dark brown finish, flowing over the ash swirl accents, features a gentle distressing that perfectly complements the dark bronze colored hardware. Bring grand traditional style to life with the sophisticated details of the Key Town Collection.
Traditional Furniture takes its beginning from furniture produced in Europe in 17-19th century. Typical traditional furniture can be identified by graceful shapes, elaborate carvings, ornamentation and details that give it a formal look. Popular traditional style types are Victorian, Neoclassic, Country, French Country, and British Colonial.Contemporary Furniture style, sometimes also described as high-tech and minimalist, uses straight lines, minimum number of colors (solid or patterned) and generally features new materials and cutting-edge technology. Popular contemporary style types are Deco, Modern, Scandinavian, Italian and Minimalism.Transitional Furniture style walks the line between traditional and contemporary furniture, with lines that are less ornate than traditional styles but not as severely basic as contemporary lines.
The Key Town Splatback Arm Chair (Set Of 2) features a sophisticated finish and ornate details to create traditional designed furniture that is sure to enhance any home's decor. With the ornate pierced chair splats and the shapely turned legs, this furniture is a shining example of inviting traditional design. Bring grand traditional style to life with the sophisticated details of the Key Town Dining Room Collection.
Made with select veneer, Ash swirl accents and hardwood solids. Dark brown, gently distressed finish. Ornate pierced chair splat. Shapely turned legs on chairs.
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Credit: Waterford Township
Challenge: Finishing a 17,000 residential meter change-out program, a $1.5 million project financed with an EPA Drinking Water State Revolving Fund loan. The contractor is swapping existing meters with Neptune Technology Group Inc. high-resolution E-Coder meters and installing Neptune's R450 radio transmitters at customer buildings. The contractor verifies in the field that the new meter's communicating properly, via the radio, to our Neptune N_Sight fixed-based network reading system and then moves to the next location. It's then our responsibility to update the data in Neptune's Customer Information System software, verify the meter's reading properly, set up alarming on the new device, and prepare the account for the next billing cycle.
Solution: With the contractor installing 300 units a week, we've reassigned staff to handle the deluge of daily installations. The project is to be completed by the end of 2014. By then, all residential meters will be read utilizing a radio-read fixed-base network, eliminating the need to read meters in the field. Customers will have increased access to their own meters, allowing low- and high-flow notifications and real-time reading capability.
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Ann Jarvis
Ann Maria Reeves Jarvis (September 30, 1832, Culpeper, Virginia — May 9, 1905, Philadelphia, Pennsylvania) was a social activist and organizer during and after the American Civil War. She and her daughter, Anna Marie Jarvis (1864–1948), are recognized as the founders of the Mother's Day holiday in the United States.
Ann Marie Reeves Jarvis was born in Culpeper, Virginia, on September 30, 1832, the daughter of Josiah Washington Reeves and his wife, Nancy Kemper Reeves. Her mother's Kemper ancestor, Johannes Kemper, came to Virginia in 1714 as one of the original German colonists whom Virginia Lt. Governor Alexander Spotswood settled at Fort Germanna on the frontier. Johannes (or John) Kemper, the immigrant, married Alice Catherine (Ailsey) Otterbach, a fellow 1714 immigrant from the Siegerland, soon after their arrival in Virginia.
Ann Marie Reeves' father, Josiah Reeves, was a Methodist minister who was transferred in 1843 from Culpeper to Philippi, Barbour County, now in West Virginia, when Ann Marie was eleven. In 1850, Ann Marie Reeves married Granville Jarvis, son of a Baptist minister, who became a successful merchant in nearby Taylor County. Anne Marie Reeves Jarvis was mother to eleven children, but only four reached adulthood.
Mrs. Jarvis was a dynamic woman who saw needs in her community and found ways to meet them. Starting in 1858, she organized Mothers' Day Work Clubs in the towns of Grafton, Pruntytown, Philippi, Fetterman, and Webster to improve health and sanitary conditions. These clubs raised money to buy medicine and to hire women to work in families where the mother suffered from tuberculosis. They developed programs to inspect milk, long before there were state requirements. Mrs. Jarvis called on her brother, Dr. James Edmund Reeves, who practiced medicine in Philippi and Fairmount, to provide advice and training for the women in her clubs.
Jarvis' activism had personal roots; of the eleven children to whom she gave birth, just four survived to adulthood. The other seven are believed to have died from diseases such as measles, smallpox, diphtheria, whooping cough, or tuberculosis—diseases now largely prevented by childhood vaccination.[1]
During the American Civil War (1861-1865) sentiment in western Virginia was sharply divided and the western part of the state broke away from Virginia and formed the new state of West Virginia, loyal to the Union. Ann Marie Jarvis urged her Mothers' Day Work Clubs to declare neutrality and to provide aid to both Confederate and Union soldiers. The clubs fed and clothed soldiers from both sides stationed in the area. When typhoid fever and measles broke out in the military camps, Mrs. Jarvis and her club members provided nursing help to the suffering soldiers, both Blue and Gray.
At the end of the war, public officials, seeking ways to eliminate postwar strife, called on Mrs. Jarvis to help. She and her club members planned a "Mothers Friendship Day" for all soldiers from both sides and their families at the Taylor County Courthouse, with bands playing "Dixie" and the "Star Spangled Banner" and "Auld Lang Syne." This effective and emotional event reduced many to tears, and to the understanding that old animosities were destructive and must end. The Mothers Friendship Day was an annual event for several years, until tensions had disappeared and it was no longer needed.
Mrs. Jarvis taught Sunday School for a quarter century, and was often invited to lecture on subjects such as "Literature as a Source of Culture and Refinement," "Great Mothers of the Bible," and "The Importance of Supervised Recreational Centers for Boys and Girls," a very progressive idea at the time. She often spoke of her dream to have a day in which Americans would honor mothers. After her husband Granville Jarvis died, she moved to Philadelphia to live with her son and two daughters. She died there in 1905.
On May 12, 1907, Jarvis' daughter, Anna Marie Jarvis, passed out 500 white carnations at her mother's church, St. Andrew's Church in Grafton, West Virginia—one for each mother in the congregation. The following year, she held a memorial to her mother in Grafton, West Virginia on May 10, 1908, and then embarked upon a campaign to make Mother's Day a recognized holiday, a goal which was achieved when President Woodrow Wilson declared it so in 1914.
See also[edit]
Nine years after the first official United States Mother's Day, commercialization of the holiday became so rampant that Anna Jarvis herself became a major opponent of what the holiday had become and spent all her inheritance and the rest of her life fighting what she saw as an abuse of the celebration.
Later commercialization and other exploitations of Mother's Day infuriated Jarvis and she made her criticisms explicitly known the rest of her life. She criticized the practice of purchasing greeting cards, which she saw as a sign of being too lazy to write a personal letter. She was arrested in 1948 for disturbing the peace while protesting against the commercialization of Mother's Day, and she finally said that she regretted having started it.
(Taken from Wikipedia's Mother's Day page: Mother's Day)
- ^ Mother's Day for Peace article from May 12, 2013; retrieved at zinnedproject.org on March 12, 2014
- Kendall, Norman F. (1937), Mothers Day, a History of its Founding and its Founder. Grafton, W. Va., D. G. Smith
- Wolfe, Howard H. (1962), Mothers Day and the Mothers Day Church. Published by the author, printed at Kingsport, Tenn. by Kingsport Press
- "Mother's Day: A Celebration rooted in a Germanna mother's life" Culpeper Star Exponent, May 14, 2012
Further reading[edit]
- LEIGH Eric Schmidt (1997). Princeton University Press, ed. Consumer Rites: The Buying and Selling of American Holidays (reprint, illustrated ed.). pp. 256–275. ISBN 0-691-01721-2.


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Spring 2014 Courses
Several schools/colleges offer honors sections of courses. Honors College students also may take honors sections of courses in order to fulfill honors diploma requirements. Please click here for a listing of honors courses that are open to students of all majors.
Honors College students will have the opportunity to enroll in interdisciplinary seminars, HONR courses, which are taught by top-notch faculty members who have been recognized for teaching excellence. We are pleased to offer 12 HONR courses for spring 2014. Please note the HONR 199 courses are for first and second-year students only; HONR 299 and 399 courses are open to all high ability students.
Below is a listing of the current semesters course offerings. Click on the course name to view a short description of the course. To download a print friendly PDF, click here.
- "Brain Disorders: Biological Basis, Treatability, Attitudes and Public Policy"
HONR 19900, Section 001, CRN 20654 - "Science and Pseudoscience: An Adventure into Logical Thinking and Inquiry"
HONR 19900, Section 002, CRN 20655 - "Youth Civic Identity in Emerging Democracies"
HONR 19900, Section 003, CRN 69815 - "Economics in Literature, Drama and Art"
HONR 19900, Section 004 CRN 69816 - "Publishing Bootcamp"
HONR 19900, Section 005, CRN 69832 - "Animals in Global Culture"
HONR 19900, Section 006, CRN 12186 - "What is Place? An Interdisciplinary Approach to Place, People and Writing"
HONR 19903, Sections 001 & 002, CRNs 69810 & 69811 - "Rise and Fall of the American Empire"
HONR 29900, Section 012, CRN 58217 - "Making the Human"
HONR 29900, Section 017, CRN 69827 - "Science Theater: Science & Technology"
HONR 29900, Section 018 CRN 69830 - "Sustainable LEED-ership"
HONR 29900, Section 019, CRN 69855 - "Photography & Cultural Value"
HONR 29900, Section 020, CRN 10303 - "Introduction to Visual Studies"
HONR 39900, Section 001, CRN 69750
"Brain Disorders: Biological Basis, Treatability, Attitudes and Public Policy"
HONR 19900, Section 001, CRN 20654
Instructor: Professor Emeritus Joseph Vanable
# of Credit Hours: 3
Days and Times: T/TH 10:30 - 11:45
Room: BRNG B254
Brief Course Description:
Brain disorders are biologically-based illnesses that affect 8 in 100 of the population, and account for far more person-hours lost to disability than can be attributed to cancer, diabetes, or heart disease. They can be treated with more success than can heart disease (success rate for schizophrenia is 60%, major depression is 70%, and bipolar disorder is 80%, heart disease treatment success rate is 51%), yet there is a great deal of stigma directed towards these illnesses, and public policy is typically crafted as if it is not possible to have effective treatment for them, and funds expended in treating them should be minimized, because it is mistakenly thought that they are essentially wasted funds.
This course will examine these and other issues associated with brain disorders, by a combination of lectures, discussion, reading primary and secondary sources (but not text books), writing research reports, and presenting them. The sole prerequisite is to have an open and enquiring mind. The approach will combine biology, psychology, medicine, law, and public policy, beginning with the fundamentals, and building on these to develop a broad and reasonably deep interdisciplinary understanding of what the present status quo is, and how this might evolve in the future.
"Science and Pseudoscience: An Adventure into Logical Thinking & Inquiry"
HONR 19900, Section 002, CRN 20655
Instructor: Professor Emeritus Alan York
# of Credit Hours: 3
Days and Times: T/Th 12:00 - 1:15
Room: SMTH 201
Brief Course Description:
Daily we are barraged with information of an astounding nature: health claims, extra-terrestrial encounters, miracles, and nutrition and diet claims, to name a few. Newspapers, magazines, television "news", and public speech present us with information that simply does not stand up to the rigor of scientific or logical examination. In this course, we will examine the basis of science and scientific thinking and the rigor and ethics that are a part of science, and apply this knowledge to many of the weird claims with which we are confronted daily. Through readings, video presentations, personal appearances, and discussion we will examine why people, educated and uneducated, make assumptions of fact that lead to belief in weird things. We will concentrate on using the precepts of science and scientific thinking to critically examine extraordinary "events", paranormal events, and pseudoscience.
"Youth Civic Identity in Emerging Democracies"
HONR 19900, Section 003, CRN 69815
Instructor: Dr. Kathy Obenchain
# of Credit Hours: 3
Days and Times: T/TH 4:30 - 5:45
Room: BRNG B254
Brief Course Description:
This seminar-based course focuses on contemporary issues, specifically how young people in emerging democracies are creating a civic identity. Unlike young people who were socialized and educated in established democracies (like the U.S.), these young people are figuring out what it means to be a civic being in a democratic nation at the same time their parents, teachers, and political and social institutions are trying to figure it out. Using primary and secondary sources from a variety of disciplines (e.g., history, political science, journalism), we will individually and collectively explore youth civic perspectives from around the world. At the same time, students will also explore their own civic identities. As an interdisciplinary course within the Honors College, students will write regularly during the semester, complete substantial writing projects, and receive feedback from the instructor to help improve your writing. Students will also have the opportunity to revise one or more assignments, and to read and discuss peers' work. Expect a substantial portion of your grade to come from your written work.
"Economics in Literature, Drama and Art"
HONR 19900, Section 004 CRN 69816
Instructor: Dr. Michael Watts
# of Credit Hours: 3
Days and Times: MW 10:30 - 11:45
Room: RAWL 2077
Brief Course Description:
Alfred Marshall described economics as the study of the "ordinary business of life," so it is really no surprise (except perhaps to many economists) that literature, drama, and art often deal with economic concepts, themes, and issues. In an anthology published in 2003, The Literary Book of Economics, I included over 70 literary passages dealing with a wide range of economic topics, written by authors from many different centuries and nations, reflecting the same range of political and personal values that one can find among economists—from Marxist to free market, but mostly in between. That anthology will be one of two key textbooks in the class – the other is a short, non-technical introduction to economics. I will provide both books for you, so don't buy anything. (Christmas comes early, for once.) We will also look at a thousand or so paintings that deal with economic concepts, issues, and themes, from a website I have set up on Art and Economics [https://intra.krannert.purdue.edu/sites/econandart/Pages/Home.aspx], which is still in development. "Genre" art deals with scenes from everyday life, so again it is not really surprising that many painters and engravers have depicted economic behavior and issues in some of their work. There will be two exams, a term paper, and two or three short class presentations.
"Publishing Bootcamp"
HONR 19900, Section 005 CRN 69832
Instructors: Charles Watkinson and Catherine Fraser-Riehle
# of Credit Hours: 3
Days and Times: T/TH 3:00 - 4:15
Room: BRNG B254
Brief Course Description:
The purpose of this course is to expose students to the world of scholarly publishing and communication, from practical issues to philosophical challenges. It will provide a competitive advantage for any student interested in pursuing publishing professionally or who plans to proceed to graduate school and especially those planning academic careers.
Scholarly publishing is a global business worth $26 billion annually and employing over 110,000 people. Despite its size and profitability, the industry currently is facing a number of disruptive forces. These include the increasing demand for free and immediate access to taxpayer-funded research, the migration of reading habits from print to digital formats, and the rise of new competitors such as the self-publishing movement and "predatory publishers." Through discussion and debate we will explore the complex ethical, technological, and commercial challenges faced by all participants in the scholarly communication cycle.
To fully understand the publishing process, one needs to live it. A tangible output of the course will be an original, peer-reviewed, high quality online and print book publication that will be entirely produced by the students enrolled. We will work together through acquiring the content, editing and design, and marketing and distribution, particularly exploring way in which digital technologies have opened new opportunities but also created new challenges.
The content for the spring 2014 semester will be a collection of biographical essays about the Purdue class of 1904, each written by a student in the Fall 2013 "Interdisciplinary Approaches to Writing" HONR 19903 class. Students also will be involved in the production of a journal, the Journal of Purdue Undergraduate Research.
"Animals in Global Culture"
HONR 19900, Section 006 CRN 12186
Instructors: Dr. Mark Russell
# of Credit Hours: 3
Days and Times: T/TH 9:00 - 10:15
Room: REC 121
Brief Course Description:
This course is an introduction of the importance of animals in various cultures and societies of the world. Factors which influence the role of animals in society including physical and biological adaptations of animals and the role of traditions, culture, religions, language, politics, geography, climatic, and socio-economics are discussed. The disciplines related to anthropology, agriculture, and biology will be integrated as we explore the societal tensions and current issues surrounding animal-human interactions. A high level of student led discussions and theme writings are expected.
"What is Place? An Interdisciplinary Approach to Place, People and Writing"
HONR 19903, Sections 001 & 002, CRNs 69810 & 69811
Instructor: Adam Watkins
# of Credit Hours: 3
Days and Times: MWF 8:30 - 9:20 and MWF 9:30 - 10:20
Room: BRNG B254
Brief Course Description:
"There are these two, young fish swimming along. They happen to meet an older fish, swimming the other way, who nods at them and says, 'Morning boys. How's the water?' The two, young fish swim on for a bit and then eventually one of them looks over to the other and says, 'What the hell is water?'" – David Foster Wallace
In a commencement speech to Kenyon College graduates, David Foster Wallace offered this now "viral" parable of daily life, which highlights the difficulty we often face when we try to talk and think critically about things that are so obvious that we are blind to them, things so important that we neglect them entirely. Place is one such thing.
This course offers an interdisciplinary approach to place, building on what Honors College students have already learned in regards to an environmental view of the world. In addition to environmentalism, we will read works of ethnography, anthropology, nature writing, cultural geography, topoanalysis, as well as place-oriented journalism and documentaries. In other words, we will consider numerous theoretical and literary approaches to the interrelationships between place, individuals, and culture.
In particular, the course will interrogate how people behave in certain spaces, why people create emotional connections with their localities, and how place can even shape who people are and how they think. Of course, we have at our disposal one of the most fascinating types of place in American culture – the college campus – and we will use this place and the people who inhabit it as our own "object of inquiry" as we test out the diverse "lenses" or ways of looking at the world noted above.
By exploring these various fields of study, students will learn numerous research strategies and styles of writing, which they will then model in their own written work. Over the last half of the course, students will propose and develop a multi-media project that incorporates one or more of these approaches to place.
This course *may* be used as a substitute for English 106 or 108. Consult your college advisor.
"The Rise and Fall of the American Empire"
HONR 29900, Section 012, CRN 58217
Instructor: Dr. Steve Hallett
# of Credit Hours: 3
Days and Times: MWF 10:30 - 11:20
Room: BRNG B254
Brief Course Description:
The major hot-button topics related to this course are taught in various departments and colleges throughout the university. Rarely, however, do we consider them together. As a result, engineers may develop a deep understanding of the oil industry without grasping the importance of global warming, and environmental scientists might study global warming without understanding the oil or coal industries. Likewise, agriculture students might discuss bio-fuels without understanding the economics of their implementation. Economists might recognize the impact of Chinese manufacturing on the global economy without recognizing its impact on the environment. This course, rather ambitiously, attempts to join the dots.
Another important goal of the course is to look at how the various global challenges will play out in different parts of the world. Overpopulation, for example, is a global problem, and yet the populations of most of the developed world are rather stable; the populations of Japan and Germany are shrinking. The political will to implement fuel efficiency standards and sources of renewable energy is strong in Europe, growing in the USA, and yet virtually non-existent in China and India. Global warming is likely to be disastrous in flood-prone Bangladesh, but may improve farming conditions in Canada.
We will take a trip through history, analyzing the relationships between human societies and their environment. We will discuss the emergence of farming and the early evolution of societies, and study the role of environmental exploitation in the demise of various civilizations. We will discuss the history of fossil fuels, the ways in which they have transformed the world, and the geopolitics of fossil fuel acquisition. Looking at the modern world, we will investigate the full range of growing global problems related to population, fossil fuel depletion, global warming, and food production. We will also analyze potential remedies, such as renewable energy (and nuclear energy). Importantly, we will discuss all these topics in the context of political and economic constraints. Perhaps the really important questions are not whether we can "save the world", but rather whether we will…
"Making the Human"
HONR 29900, Section 017, CRN 69827
Instructor: Dr. Dino Felluga
# of Credit Hours: 3
Days and Times: T/TH 12:00 – 1:15 PM
Room: BRNG B254
Brief Course Description:
This course asks the question: how did we come to be who we are? To answer that question, we will examine what is arguably the most important moment and place for the modern understanding of the human in the Western world: Renaissance Italy. How did the innovations of the Italian (and especially the Venetian) Renaissance lead us to a new understanding of ourselves, one that not only ushered in the modern era but also continues to shape us today? All aspects of the human will be explored: visuality, space, time, psychology, knowledge, faith, government, art, architecture, music, science, urban space, technologies of communication, discipline and punishment. This course will have a companion 2-credit course in which students can also enroll: HONR 299—Revisiting Venice. Revisiting Venice will take students all the way to Venice, Italy, where we will see and hear firsthand the art, architecture, music, and scientific artifacts that made the Renaissance the most important epoch in Western human history. Work for the 2-credit study visit will involve keeping a travel journal of ideas that engages with what we discuss and see during our visit to Italy.
"Science Theater: Science & Technology"
HONR 29900, Section 018, CRN 69830
Instructor: Dr. Tom Turpin
# of Credit Hours: 3
Days and Times: T/TH 1:30 - 2:45
Room: BRWN 3104
Brief Course Description:
This class will explore the relationship between scientific discovery and the relationship to technological innovation. The class will visualize and develop a theatrical performance included scripts and props, and participate as actors. The target audience will be students in Middle School. Final products for the class will be video clips and a live performance of the material before a live audience.
"Sustainable LEED-ership"
HONR 29900, Section 019, CRN 69855
Instructors: Michael Gulich and Michael Ursem
# of Credit Hours: 3
Days and Times: T-TH 1:30 - 2:45
Room: BRNG B254
Brief Course Description:
In the United States, buildings account for: 36% of total energy use, 65% of electricity consumption, 30% of greenhouse gas emissions, 30% of raw materials use, 30% of waste output, and 12% of potable water consumption (USGBC). In order to meet the global challenges of our time, a more resourceful approach to engineering, architecture, and infrastructure will have to be developed that reduces the inputs of energy, water, and materials and while also reducing the outputs of waste and emissions. Several frameworks have emerged to facilitate this shift including the LEED green building rating system, the Living Building Challenge, the Sustainable Sites Initiative, and the Envision Sustainable Infrastructure Rating System, among others. As these systems evolve, design and construction professionals and product manufacturers continue to raise performance levels incrementally.
This course will introduce these different rating systems with relevant case studies and also evaluate the strengths and weaknesses of each system. Guest speakers who practice green design and construction will be invited to offer diverse perspectives and 'real-time' case studies.
"Photography & Cultural Value"
HONR 29900, Section 020, CRN 10303
Instructor: Dr. Rosanne Altstatt
# of Credit Hours: 3
Days and Times: T/TH 9:00 – 10:15 AM
Room: BRNG B254
Brief Course Description:
The source material for this course is the studio photography of Charles Pansirna, housed in the Purdue University Galleries collection. These images will come under intense scrutiny as students learn how to look by viewing more photos each week and discussing their cultural value. This is less a matter of assessing and assigning value than of creating it. We will take the multiple perspectives of visual culture studies: visiting archives, devising modernist and postmodernist taxonomies for these photos, researching their histories, slipping them into art historical comparisons, imitating and inhabiting them as part of a curatorial methodology. We will do so with an eye toward a future exhibition at Purdue Galleries. Individual students' research will feed a wiki made by the class with the ultimate goal of bringing Pansirna's photos from glass plate negatives to images in the digital circulation of a global context.
The generic space of the vernacular studio is a confined, local space that is present most everywhere around the world. It hosts the request of non-celebrities to be photographed: rites of passage in various religions and cultures are marked, families dress in September for Holidays cards or one last formal photo is taken before a son or daughter is shipped overseas. Newborns, newlyweds and even the dead in their caskets pause to be arranged and posed by the studio photographer.
How do we as scholars approach the vernacular photography of Pansirna Studios, frequented by members of Chicago's vibrant Lithuanian-American community between the years of 1916 and 1953? As historical documents: Pansirna's community has since dissolved into the American melting pot, its members passed away, their images now haunting over 1600 photographic negatives and prints in the collection. As art history: We consider how these photos compare to renowned studio photography from the USA, UK and Mali that was either intended for the field of art or has been brought into it by curators and scholars. As ethnography: We ask why these photos are sometimes considered ethnographic and search the images for evidence that may or may not support this problematic category. As material: We will study how these photos were made, their technical specifics and the mode of being a photographer or a sitter. This will extend into the impact of material forms upon the viewer. How does the viewing of negatives, the prints drawn from them or their digitization and subsequent malleability affect their reception?
"Introduction to Visual Studies"
HONR 39900, Section 001, CRN 69750
Instructor: Dr. Christopher Lukasik
# of Credit Hours: 3
Days and Times: MW 4:30 - 5:45
Room: BRNG B254
Brief Course Description:
Ours is a predominantly visual culture. The ever-expanding array of new visual technologies and the practices of vision they generate influence how we see ourselves and our world, and how we think about seeing itself. With the visualization of knowledge more and more integral to the functioning of all advanced professional societies, the visual has been increasingly seen as the key to solving imperatives of speed and efficiency in "The Information Age" and images have become central to how humans connect to technology and to one another. Yet, most students enter and exit Purdue without even the most basic knowledge of the images that have come to play such a vital part of their daily experience. This course will change that by introducing students to the emerging field of visual studies. It will provide them with an overview of the basic theories, practices, and histories of the visual from a variety of disciplinary perspectives and historical periods from the physical mechanisms of sight (such as the anatomical structure of the eye and the neurological basis of vision in the brain) to the historical and social dimensions of seeing (such as theories of the gaze, spectacle, and surveillance). The course will be organized around a series of basic questions (such as what is an image? how do images produce meaning? what is vision?) whose responses have come to constitute the field of visual studies as well as its interdisciplinarity. Students will learn to understand how images and their viewers make meaning, to determine what role images play in our cultures, and to consider what it means to negotiate so many images in our daily lives. In short, the course will encourage students to approach critically the way we engage (and have engaged) the world in terms of what we see and what we want to see. Readings will be drawn from seminal works in visual theory, critical iconology, cultural semiotics, cultural theory, intermediality, art history, media studies as well as the psychology, philosophy, and science of vision. Course format will be lecture/discussion.


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RubyMine 4.5 Released
Congrats to the Jetbrains team for another great looking release. As always you can hit the What's New page for all the new bits. A few highlights:
- SASS, Slim and Sinatra
- MongoDB and Mongoid
- Rails Engines
- Capistrano
- (My favorite) "Renaming model magic" for more awesome refactoring
- Improved Mac support with native help and retina display support
Note that 4.5 is a free update for everyone who purchased their RubyMine license after July 18, 2011.


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Man Up Mondays Episode 1
A Snowboarding video by survivingthewinter - 1,976 views
This isn't a very hard trick, and we aren't particularly good freestyle snowboarders. But that's pretty much the whole point of Man Up Mondays. To try something you would never normally try on your own when the cameras aren't rolling.
This week, Tom attempts to get Paul off his backside and try something different instead of nosepressing every which way around the slope.
Follow us on facebook if you want more content


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This coverall is suitable for helping protect workers who face the challenges of being exposed to non-hazardous light aerosols and dry particles in many industrial applications.
This Web site is intended for use by US residents only. See International Sites. See our delivery policy for full details. Copyright 2013, Staples, Inc., All Rights Reserved.


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Fiber is essential for a healthy body. In fact, most adults require 32 grams of fiber each day to maintain digestive health, regularity, a healthy weight, and most importantly, a healthy heart! However, most Americans only get about half this amount in their diet, and usually less.
This lack of fiber in the typical American diet may contribute to the rising rates of heart disease, obesity, and digestive problems that plague the country. Taking a fiber supplement each day is the easy way to ensure you are getting all you need to maintain your health. The only drawback? Unlike your fruits, vegetables and grains, fiber supplements were not certified organic... Until now!
Watch this video to learn why it's so important to choose organic psyllium, or read this blog.
If your goal is to soothe your digestive system, and support your heart then it's essential to start with the cleanest, organic herbs!
Our psyllium is USDA certified organic. This means that from the time the seeds are planted until the moment you purchase the final product, you have the assurance that our psyllium is grown without pesticides, herbicides or chemical fertilizers, contains no additives or sweeteners and is of the highest quality available.
In whole husk form, derived from the seeds of the herb Plantago ovata, psyllium husks are a rich source of soluble fiber. They naturally promote healthy elimination and regularity while supporting the gastrointestinal system.
Heart Healthy! Psyllium has also proven to be quite substantial in the lowering of both total cholesterol and LDL cholesterol level**.
There are two types of fiber: Soluble and insoluble.
Soluble fiber absorbs water and forms a gel-like substance which entraps food, sugars, cholesterol and fats in the stomach and carries them through the digestive tract.
Psyllium is a great source of soluble fiber!
Insoluble fiber does not dissolve in water. It moves through your digestive system quickly and mostly in tact, acting as a bulking agent and improving regularity.
Psyllium is one of the only fiber supplements that contains a beneficial amount of both soluble and insoluble fiber!
Fiber has no calories because it is not digestible and is not absorbed into the body.
Most animal products including meat, eggs, milk and cheese don't contain any fiber.
Fiber offers many health benefits, especially the fiber found in psyllium! Not only does the insoluble fiber in psyllium act as a bulking agent to improve digestive regularity, its soluble fiber content blocks fat and cholesterol absorption, making it essential to heart health.
Filling up on high fiber foods or taking psyllium as a fiber supplement may also help you feel full for a longer period of time and leave you with less room for less healthy food options.
Here's a great recipe for Cacao Cakes with Psyllium!
Psyllium Smoothies! Check out this video for a great recipe using ORGANIC INDIA organic Psyllium and Tulsi Tummy Tea!
ORGANIC INDIA was the first company to produce and market organic psyllium. We have advanced cleaning techniques that allow us to do what was once thought impossible - produce certified organic psyllium!
We use a dry steam process to clean our organic psyllium. Conventional psyllium is commonly cleaned via a fumigation process using methyl bromide, then sterilized with ethylene oxide.
We use organic crop rotation practices. Conventional crops are commonly rotated with castor, cumin, mustard, and wheat, which are treated with chemical pesticides, herbicides, and fertilizers. These pesticides are hazardous to the health of farmers and increase chemical toxicity in soil, water and air.
Every purchase of ORGANIC INDIA psyllium contributes to the sustainable livelihood of the family farmers we work with in India!


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Scarlett Rouge, my favorite blonde bbw bimbo can be such a nasty slut when she's in the mood. Yes, I love all the pics and videos where she is doing the whole glamour puss thing, but let it be known THIS GIRL LOVES SEX RAW AND NASTY
Look at those meaty pussy lips, definatly some meat curtains that are ready for a hard cock to be showed between them. Look how this plump cocksucker loves the cock!


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Narrow Lot
Square Feet: 964 Bedrooms: 2
Full Bathrooms: 2Great for First-Time Buyers
That's the beauty of a bungalow. Design 24240 is all about accommodating family comfortably. Great for getaways and everyday! This one features 964 sq. ft. and a classic pitched hip roof. The fireplace lights up the living room while the kitchen warms up to a bright corner window. The kitchen panty holds plenty and while the breakfast booth gazes over the back porch the dining room muses over the front porch. The master bedroom snuggles up to a private shower room with personal toilette and vanity sink. Bedroom #2 enjoys nearby ¾ bath with tub toilette and vanity sink Learn MoreW0063
Square Feet: 1008 Bedrooms: 2
Full Bathrooms: 2This adorable 2 bedroom 2 bathroom home is great for the family just starting out. With a single car garage rear porch and private bathrooms it is sure to meet your needs. Learn Moreb-95010
Square Feet: 1042 Bedrooms: 2
Full Bathrooms: 2The double-door entry leads to a dramatically vaulted living room which overlooks a delightful rear lanai. The dining room is easily served by the kitchen which features a pantry closet. The vaulted master bedroom has a huge walk-in closet with built-in shelves and a private bath. Learn More1044-11-09
Square Feet: 1044 Bedrooms: 2
Full Bathrooms: 2This cute little plan is perfect for a young couple single professional or vacation bungalow. Craftsman detailing on the elevation invites you into a compact but easily living plan. Learn MoreDH-1080
Square Feet: 1073 Bedrooms: 2
Full Bathrooms: 2This plan is cute as a button and loaded with personality. She's adorned with shake siding stacked columns and lots of windows. A quaint porch welcomes you to this cute home. Enter the large Great Room that shares a raised snack bar with the Kitchen. The Kitchen/Dining area is open and has lots of cabinets and a pantry. A nice sized Laundry Room has access to Bath 2 for easy clean up. Bath 2 is perfect for family and guests. Both bedrooms are nice sized and have lots of closet space. Bedroom 1 has a private bath with dual sinks and linen storage. This cute home has lots of storage. This economical plan may be small but not short on style. Learn More24740
Square Feet: 1093 Bedrooms: 2
Full Bathrooms: 2Enjoy Surrounding Vistas
The side covered porch of this home encourages the occupants to enjoy the outdoors. The covered porch and great room both receive an abundance of natural light from overhead skylights. In the great room a soaring vaulted ceiling enhances the home's sense of airiness. A loft overlooks the great room and opts as a terrific computer/study area. Learn MoreW0070
Square Feet: 1094 Bedrooms: 3
Full Bathrooms: 2This economical 3 bedroom 2 bathroom Southwestern Suburbian style home is great for the family on a budget. With a large great room to cultivate family ties and open kitchen to keep everyone in on the fun you can't help but want to spend time with those you love. Learn MoreDH-1122
Square Feet: 1122 Bedrooms: 2
Full Bathrooms: 2This Charming Cottage is sure to WOW you! Are you looking to get the most for your money? Well look no further. Enter this spacious Great Room with three full-length windows to ensure maximum natural lighting. The Kitchen/Dining is fully loaded with a movable Island snack bar pantry and lots of cabinets. The Master Bedroom is nice-sized has a private bath and a great walk-in closet. Bedroom 2 is perfect for extra room and a great walk-in closet. Bath # 2 is ideally located for guests that come to relax in the Great Room. There is a great Laundry room with a covered porch off the side to give a nice balance of work and play. Learn MoreW0047
Square Feet: 1140 Bedrooms: 3
Full Bathrooms: 2This quaint 3 bedroom 2 bathroom home is perfect for the family just starting out or the empty-nester wanting to down-size. With an emphasis on the large front porch this home is also great for a social lifestyle. Learn MoreDH-1157
Square Feet: 1152 Bedrooms: 2
Full Bathrooms: 2The Perfect Cottage! The covered front porch welcomes you into this country cottage. The Great Room features a wall of Built-in entertainment unit and upper/lower cabinets for give unexpected storage. The Kitchen/Dining area is open and airy - perfect for entertaining friends and family. A large pantry can be found close to the kitchen and laundry room. A French door leads out onto a rear covered porch. A nice sized Laundry room is located with-in steps from the Kitchen and within easy access from the rear porch. The Master Bedroom has a nice Walk in Closet Private Bath and has access to the Rear Covered Porch. Bedroom # 2 is a nice size with a Walk in Closet. Learn MoreW0065
Square Feet: 1162 Bedrooms: 3
Full Bathrooms: 2This cute 3 bedroom 2 bathroom craftsman home is a great cottage or lake home. With large porches on both the front and back of the home you can soak up nature around you. This home works well with both narrow lots and on hillsides. Learn Morepd1163-28-10
Square Feet: 1163 Bedrooms: 3
Full Bathrooms: 2Plan is perfect for historical in-fill areas or other narrow lots. Darling craftsman bungalow elevation features an inviting front porch & dormer. Lots of closets & easy to furnish rooms make this plan a great starter or retirement home. Learn More24241
Square Feet: 1174 Bedrooms: 3
Full Bathrooms: 2Nice and Comfortable
The covered porch welcomes visitors and family alike. A spacious living room with a fireplace adds to the cozy atmosphere. In the warmer months the house is kept cool due to its typical bungalow design that allows heat to collect in the attic space and away from the areas of activity. Learn More


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Most everyone will not need an introduction to our oldest son Chad Anton...who just celebrated his 33rd Birthday, and is well known ( and loved) over the years to most of our guests on Promenade.
Chad has been involved in family businesses since he was very small. He started helping out when I was involved with Hotels and Dinner Theaters, and spent a lot of time learning every role possible when we owned our family restaurant for 7 years while a teenager. He has spent seasons working on Yacht Promenade in his younger days, and has consistently came back to help out when I needed him too.
In between this, he lived in Canada, opening up several bars, restaurants and nightclubs, and also became Regional Director of one of the top ten franchise companies (Vanguard Cleaning..which means the boat should always be spotless!).
Chad, several years ago, became involved with the charter brokerage business as a broker in SailDiveBVI, and in the last 6 months brought us a proposal for an unique, one of a kind online yachting and water activity business that would fit in very well with our current business.
Although not quite ready to go public with our new addition company wise as of yet, I will say that we have spent many months in development and it is progressing nicely. I can give you a teaser
We have built up our yachting business over 15 years, and Chad always wanted to take it over eventually, although his time frame was a couple years away. With no wife nor children for him on the horizon, and the increased workload of developing a new company, I figured there really was no time like the present. Chad joining us, will enable him to focus on one industry (yachting) as opposed to several that he is doing now. It will allow Bazza and I the ability to transition and cycle out of actively crewing the charters in the future, and work on less physical aspects of our businesses.
Chad will be working full time with the company starting November, and with already holding his SCUBA Dive Master certificate, and a Yachtmaster ticket, is eager to "get on with it"- in whatever aspect of the company is needed. Look for him to be on charters around the New Year, and by next spring, he should be captaining most of the actual charters on Promenade, and actively managing all it entails.
Chad, Felix and Lindsey will do a spectacular job, and unlike us, they are WANTING to run a busy schedule over the next few years and indeed, offer charters for 10 months of the year. Great news for our guests!
We have some special rates up for open weeks this calendar year...and I know everyone will be extremely pleased with our charters and crew.
Here are some pictures of Chad and us over the years on Yacht Promenade! yeah, some of them go back a long long way!
Chad Anton can be contacted at email@example.com or email@example.com
I am a pretty happy mother and proud.


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New York Demands Apology From Lil Wayne
Lil Wayne recently released a statement about his dislike for New York, however he never thought that anyone would respond back to his statement.
Read moreLil Wayne recently released a statement about his dislike for New York, however he never thought that anyone would respond back to his statement.
Read moreJuvenile is no stranger to the jail house, but the rapper sure did not expect to get booked recently after fighting at a hotel.
Read moreBow Wow has been signed to Young Money for quite some time now and many artists say the greener in the Young Money team, however that may not be the case for all of its member.
Read morePusha T and Lil Wayne recently reignited their beef, and now 2 Chainz is left to take sides expect he refuses to take sides. 2 Chainz recently explained why the beef will not break his ties to artists from either…
Read moreWhen Lil Wayne spoke his mind about Peter Rosenberg's beef with Nicki Minaj, Peter just fought back.
Read morePusha T recently dissed Drake and Lil Wayne just reacted by expressing what he had to say.
Read moreLil Wayne explains that he removed Nicki Minaj from Summer Jam, because she was being disrespected.
Read moreLil Wayne collaborates with Supra Footwear and he's ready to release his own line of shoes.
Read moreLil Wayne is going on a new mission as he teams up with DJ Scoob Doo for the launch of a new website called BreadOverBed.com.
Read moreFlo Rida and Lil Wayne recently hooked up to work on a new track that left Flo Rida amazed. Both artists have teamed up for a track on Flo's new album Wild Ones. He loved being in the studio with…
Read more


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B&Bs in Limoges-Rochechouart, France
Choose from our range of B&B's in Limoges-Rochechouart. View photos of B&B's in Limoges-Rochechouart and read bed and breakfast reviews. To find a B&B in Limoges-Rochechouart in the right location for you, view all of the Limoges-Rochechouart B&B's on the map and book online.
La Belle Vienne
Le Bourg ExideuilLa Belle Vienne is a traditional French village house set in a village on the banks of the beautiful River Vienne, just 30 mins from Limoges airport and 13km from the Lakes of the Haute Charente. Recently renovated and tastefully decorated by
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La Maison Rouge Volets
La Coldebouye, Exideuil sur VienneOur period village house, on the edge of Exideuil sur Vienne, is full of character but benefits from modern ensuite bathrooms and central heating. We offer relaxed, comfortable bed and breakfast accommodation in the heart of the Charente Limousine
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Maison Chabaudie
6 Allee de la chabaudie, RochechouartExperience hidden France at Maison Chabaudie, a family-run B&B offering a warm welcome and a relaxing feeling. 35km from Limoges Airport.
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- price per room per night


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Main Content
Ricky Stenhouse, Jr. will be changing gears in his rookies eason, racing full-time in the NASCAR Sprint Cup Series™. The two-time NASCAR Nationwide Series™ Champion will pilot the No. 17 NOS Energy Ford Fusion for one race this season. A proven winner and a fierce competitor, Stenhouse is sure to charge to the front for a first-place finish! This 1:64 scale car features a die-cast body, high-quality paint, and custom decals similar to the graphics on your favorite driver's car. Race fans do not want to miss their opportunity to own the Action Racing Collectibles die-cast of the No.17 NOS Energy Ford Fusion. Created to replicate the brand new paint scheme and showcase the Generation 6 stock body, each die-cast has precise detail and is officially licensed by NASCAR®.
- 1:64 Scale (approximately 3" in length)
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- Officially licensed NASCAR product
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Special event items are produced by manufacturers only after the outcome of a game
or event. These are advanced sale items and will ship immediately after they are
received in our warehouse.
Manufacturer direct items are shipped directly from the manufacturer. These items
are not available for international or expedited shipping.
Customized items can be personalized with options such as your name, your favorite
number, and/or designs. Some options may be limited by league rules.


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Dynamic Protein Domains: Identification, Interdependence, and Stability Biophysical Journal, Volume 91, Issue 2, 15 July 2006, Pages 670-685 Semen O. Yesylevskyy, Valery N. Kharkyanen and Alexander P. Demchenko Abstract Existing methods of domain identification in proteins usually provide no information about the degree of domain independence and stability. However, this information is vital for many areas of protein research. The recently developed hierarchical clustering of correlation patterns (HCCP) technique provides machine-based domain identification in a computationally simple and physically consistent way. Here we present the modification of this technique, which not only allows determination of the most plausible number of dynamic domains but also makes it possible to estimate the degree of their independence (the extent of correlated motion) and stability (the range of environmental conditions, where domains remain intact). With this technique we provided domain assignments and calculated intra- and interdomain correlations and interdomain energies for >2500 test proteins. It is shown that mean intradomain correlation of motions can serve as a quantitative criterion of domain independence, and the HCCP stability gap is a measure of their stability. Our data show that the motions of domains with high stability are usually independent. In contrast, the domains with moderate stability usually exhibit a substantial degree of correlated motions. It is shown that in multidomain proteins the domains are most stable if they are of similar size, and this correlates with the observed abundance of such proteins. Abstract | Full Text | PDF (490 kb) |
In Silico Characterization of Resonance Energy Transfer for Disk-Shaped Membrane Domains Biophysical Journal, Volume 92, Issue 9, 1 May 2007, Pages 3040-3051 Maria A. Kiskowski and Anne K. Kenworthy Abstract Förster resonance energy transfer (FRET) has become an important tool to study the submicrometer distribution of proteins and lipids in membranes. Although resolving the two-dimensional distribution of fluorophores from FRET is generally underdetermined, a forward approach can be used to determine characteristic FRET "signatures" for interesting classes of microdomain organizations. As a first step toward this goal, we use a stochastic Monte Carlo approach to characterize FRET in the case of molecules randomly distributed within disk-shaped domains. We find that when donors and acceptors are confined within domains, FRET depends very generally on the density of acceptors within domains. An implication of this result is that two domain populations with the same acceptor density cannot be distinguished by this FRET approach even if the domains have different diameters or different numbers of molecules. In contrast, both the domain diameter and molecule number can be resolved by combining this approach with a segregation approach that measures FRET between donors confined in domains and acceptors localized outside domains. These findings delimit where the inverse problem is tractable for this class of distributions and reframe ways FRET can be used to characterize the structure of microdomains such as lipid rafts. Abstract | Full Text | PDF (626 kb) |
The Influence of Interdomain Interactions on the Intradomain Motions in Yeast Phosphoglycerate Kinase: A Molecular Dynamics Study Biophysical Journal, Volume 92, Issue 5, 1 March 2007, Pages 1709-1716 Erika Balog, Monique Laberge and Judit Fidy Abstract A 3-ns molecular dynamics simulation in explicit solvent was performed to examine the inter- and intradomain motions of the two-domain enzyme yeast phosphoglycerate kinase without the presence of substrates. To elucidate contributions from individual domains, simulations were carried out on the complete enzyme as well as on each isolated domain. The enzyme is known to undergo a hinge-bending type of motion as it cycles from an open to a closed conformation to allow the phosphoryl transfer occur. Analysis of the correlation of atomic movements during the simulations confirms hinge bending in the nanosecond timescale: the two domains of the complete enzyme exhibit rigid body motions anticorrelated with respect to each other. The correlation of the intradomain motions of both domains converges, yielding a distinct correlation map in the enzyme. In the isolated domain simulations—in which interdomain interactions cannot occur—the correlation of domain motions no longer converges and shows a very small correlation during the same simulation time. This result points to the importance of interdomain contacts in the overall dynamics of the protein. The secondary structure elements responsible for interdomain contacts are also discussed. Abstract | Full Text | PDF (741 kb) |
Copyright © 2006 The Biophysical Society All rights reserved.
Biophysical Journal, Volume 91, Issue 8, 3002-3013, 15 October 2006
Semen O. Yesylevskyy*, , , Valery N. Kharkyanen* and Alexander P. Demchenko†, ‡
* Department of Physics of Biological Systems, Institute of Physics, National Academy of Science of Ukraine, Kiev, Ukraine
† A.V. Palladin Institute of Biochemistry, Kiev, Ukraine
‡ Research Institute for Genetic Engineering and Biotechnology, TUBITAK, Gebze-Kocaeli, Turkey
Analysis of changes in the dynamics of protein domains on ligand binding is important in several aspects: for the understanding of the hierarchical nature of protein folding and dynamics at equilibrium; for analysis of signal transduction mechanisms triggered by ligand binding, including allostery; for drug design; and for construction of biosensors reporting on the presence of target ligand in studied media. In this work we use the recently developed HCCP computational technique for the analysis of stabilities of dynamic domains in proteins, their intrinsic motions and of their changes on ligand binding. The work is based on comparative studies of 157 ligand binding proteins, for which several crystal structures (in ligand-free and ligand-bound forms) are available. We demonstrate that the domains of the proteins presented in the Protein DataBank are far more robust than it was thought before: in the majority of the studied proteins (152 out of 157), the ligand binding does not lead to significant change of domain stability. The exceptions from this rule are only four bacterial periplasmic transport proteins and calmodulin. Thus, as a rule, the pattern of correlated motions in dynamic domains, which determines their stability, is insensitive to ligand binding. This rule may be the general feature for a vast majority of proteins.