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The Library
Uptake and cytotoxicity of citrate-coated gold nanospheres : comparative studies on human endothelial and epithelial cells
Freese, Christian, Uboldi, Chiara, Gibson, Matthew I., Unger, Ronald E., Weksler, Babette B., Romero, Ignacio A., Couraud, Pierre-Olivier and Kirkpatrick, C. J. (C. James). (2012) Uptake and cytotoxicity of citrate-coated gold nanospheres : comparative studies on human endothelial and epithelial cells. Particle and Fibre Toxicology, Vol.9 (No.1). p. 23. ISSN 1743-8977
WRAP_Gibson_art%3A10.1186%2F1743-8977-9-23.pdf - Published Version Download (741Kb) | Preview |
Official URL: http://dx.doi.org/10.1186/1743-8977-9-23
The use of gold nanoparticles (AuNPs) for diagnostic applications and for drug and gene-delivery is currently under intensive investigation. For such applications, biocompatibility and the absence of cytotoxicity of AuNPs is essential. Although generally considered as highly biocompatible, previous in vitro studies have shown that cytotoxicity of AuNPs in certain human epithelial cells was observed. In particular, the degree of purification of AuNPs (presence of sodium citrate residues on the particles) was shown to affect the proliferation and induce cytotoxicity in these cells. To expand these studies, we have examined if the effects are related to nanoparticle size (10, 11 nm, 25 nm), to the presence of sodium citrate on the particles' surface or they are due to a varying degree of internalization of the AuNPs. Since two cell types are present in the major barriers to the outside in the human body, we have also included endothelial cells from the vasculature and blood brain barrier. Results Transmission electron microscopy demonstrates that the internalized gold nanoparticles are located within vesicles. Increased cytotoxicity was observed after exposure to AuNPs and was found to be concentration-dependent. In addition, cell viability and the proliferation of both endothelial cells decreased after exposure to gold nanoparticles, especially at high concentrations. Moreover, in contrast to the size of the particles (10 nm, 11 nm, 25 nm), the presence of sodium citrate on the nanoparticle surface appeared to enhance these effects. The effects on microvascular endothelial cells from blood vessels were slightly enhanced compared to the effects on brain-derived endothelial cells. A quantification of AuNPs within cells by ICP-AES showed that epithelial cells internalized a higher quantity of AuNPs compared to endothelial cells and that the quantity of uptake is not correlated with the amount of sodium citrate on the nanoparticles' surface. Conclusions In conclusion the higher amount of citrate on the particle surface resulted in a higher impairment of cell viability, but did not enhance or reduce the uptake behavior in endothelial or epithelial cells. In addition, epithelial and endothelial cells exhibited different uptake behaviors for citrate-stabilized gold nanoparticles, which might be related to different interactions occurring at the nanoparticle-cell-surface interface. The different uptake in epithelial cells might explain the higher reduction of proliferation of these cells after exposure to AuNPs treatment although more detailed investigations are necessary to determine subcellular events. Nevertheless an extrinsic effect of sodium-citrate stabilized particles could not be excluded. Thus, the amount of sodium citrate should be reduced to a level on which the stability of the particles and the safety for biomedical applications are guaranteed.
Item Type: | Journal Article |
Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
Divisions: | Faculty of Science > Chemistry |
Library of Congress Subject Headings (LCSH): | Gold -- Physiological effect, Gold -- Therapeutic use |
Journal or Publication Title: | Particle and Fibre Toxicology |
Publisher: | BioMed Central Ltd. |
Date: | 2012 |
Volume: | Vol.9 |
Number: | No.1 |
Page Range: | p. 23 |
Identification Number: | 10.1186/1743-8977-9-23 |
Status: | Peer Reviewed |
Publication Status: | Published |
Access rights to Published version: | Open Access |
Funder: | Sixth Framework Programme (European Commission) (FP6), Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Higher Education Funding Council for England (HEFCE), Birmingham Science City |
Grant number: | FP6–2004–NMP–TI4–032731 (FP6) |
References: | 1. Sperling RA, Gil P, Zhang F, Zanella M, Parak WJ: Biological applications of gold nanoparticles. Chem Soc Rev 2008, 37(9):1896–1908. 2. Boisselier E, Astruc D: Gold nanoparticles in nanomedicine: preparation, imaging, diagnostics, therapies and toxitiy. Chem Soc Rev 2009, 38(6):1759–1782. 3. Sopjani M, Föller M, Lang F: Gold stimulates Ca2+ entry into and subsequent suicidal death of erythrocytes. Toxicology 2008, 244:271–279. 4. Sereemaspun A, Rojanathanes R, Wiwanitkit V: Effect of gold nanoparticle on renal cell: an implication for exposure risk. Renal failure 2008, 30:323–325. 5. Cho W-S, Cho M, Jeong J, Choi M, Cho H-Y, Han BS, Kim SH, Kim HO, Lim YT, Chung BH, Jeong J: Acute toxicity and pharmacokinetics of 13 nm-sized PEG-coated gold nanoparticles. Toxicology and Applied Pharmacology 2009, 236(1):16–24. 6. Gibson MI, Danial M, Klok H-A: Sequentially Modified, Polymer-Stabilized Gold Nanoparticle Libraries: Convergent Synthesis and Aggregation Behavior. ACS Combinatorial Science 2011, 13(3):286–297. 7. Pan Y, Leifert A, Ruau D, Neuss S, Bornemann J, Schmid G, Brandau W, Simon U, Jahnen-Dechent W: Gold nanoparticles of diameter 1.4 nm trigger necrosis by oxidative stress and mitochondrial damage. Small (Weinheim an der Bergstrasse, Germany) 2009, 5:2067–2076. 8. Arvizo RR, Miranda OR, Thompson MA, Pabelick CM, Bhattacharya R, Robertson JD, Rotello VM, Prakash YS, Mukherjee P: Effect of nanoparticle surface charge at the plasma membrane and beyond. Nano letters 2010, 10:2543–2548. 9. Hauck TS, Ghazani AA, Chan WCW: Assessing the effect of surface chemistry on gold nanorod uptake, toxicity, and gene expression in mammalian cells. Small (Weinheim an der Bergstrasse, Germany) 2008, 4:153–159. 10. Uboldi C, Bonacchi D, Lorenzi G, Hermanns MI, Pohl C, Baldi G, Unger RE, Kirkpatrick CJ: Gold nanoparticles induce cytotoxicity in the alveolar type-II cell lines A549 and NCIH441. Part Fibre Toxicol 2009, 6:18. 11. Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, Pober JS, Wick TM, Konkle BA, Schwartz BS, et al: Endothelial cells in physiology and in the pathophysiology of vascular disorders. Blood 1998, 91:3527–3561. 12. Kirkpatrick CJ, Bittinger F, Klein CL, Hauptmann S, Klosterhalfen B: The role of the microcirculation in multiple organ dysfunction syndrome (MODS): a review and perspective. Virchows Arch 1996, 427:461–476. 13. Kunzmann A, Andersson B, Thurnherr T, Krug H, Scheynius A, Fadeel B: Toxicology of engineered nanomaterials: Focus on biocompatibility, biodistribution and biodegradation. Biochimica et biophysica acta 2010, 1810(3):361–73. 14. Bartczak D, Sanchez-Elsner T, Louafi F, Millar TM, Kanaras AG: Receptor- Mediated Interactions between Colloidal Gold Nanoparticles and Human Umbilical Vein Endothelial Cells. Small 2010, 7(3):388–94. 15. Bartczak D, Muskens OL, Nitti S, Sanchez-Elsner T, Millar TM, Kanaras AG: Interactions of Human Endothelial Cells with Gold Nanoparticles of Different Morphologies. Small 2012, 8:122–130. 16. Chithrani BD, Ghazani AA, Chan WCW: Determining the Size and Shape Dependence of Gold Nanoparticle Uptake into Mammalian Cells. Nano Letters 2006, 6:662–668. 17. Ma Y-J, Gu H-C: Study on the endocytosis and the internalization mechanism of aminosilane-coated Fe3O4 nanoparticles in vitro. Journal of materials science Materials in medicine 2007, 18:2145–2149. 18. Nativo P, Prior IA, Brust M: Uptake and Intracellular Fate of Surface-Modified Gold Nanoparticles. ACS Nano 2008, 2:1639–1644. 19. Scholzen T, Gerdes J: The Ki-67 protein: from the known and the unknown. Journal of cellular physiology 2000, 182:311–322. 20. Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. Journal of immunology (Baltimore, Md: 1950) 1984, 133:1710–1715. 21. Sasaki K, Murakami T, Kawasaki M, Takahashi M: The cell cycle associated change of the Ki-67 reactive nuclear antigen expression. Journal of cellular physiology 1987, 133:579–584. 22. Dyer LA, Patterson C: Development of the endothelium: an emphasis on heterogeneity. Seminars in thrombosis and hemostasis 2010, 36:227–235. 23. Mironava T, Hadjiargyrou M, Simon M, Jurukovski V, Rafailovich MH: Gold nanoparticles cellular toxicity and recovery: Effect of size, concentration and exposure time: Nanotoxicology. Nanotoxicology 2010, 4:120–137. 24. Roa W, Zhang X, Guo L, Shaw A, Hu X, Xiong Y, Gulavita S, Patel S, Sun X, Chen J, et al: Gold nanoparticle sensitize radiotherapy of prostate cancer cells by regulation of the cell cycle. Nanotechnology 2009, 20:375101. 25. Fang JL, Beland FA: Long-term exposure to zidovudine delays cell cycle progression, induces apoptosis, and decreases telomerase activity in human hepatocytes. Toxicol Sci 2009, 111:120–130. 26. Zhang L, Zhang J, Hu C, Cao J, Zhou X, Hu Y, He Q, Yang B: Efficient activation of p53 pathway in A549 cells exposed to L2, a novel compound targeting p53-MDM2 interaction. Anticancer Drugs 2009, 20:416–424. 27. Gosens I, Post JA, de la Fonteyne LJ, Jansen EH, Geus JW, Cassee FR, de Jong WH: Impact of agglomeration state of nano- and submicron sized gold particles on pulmonary inflammation. Part Fibre Toxicol 2010, 7:37. 28. Nel AE, Madler L, Velegol D, Xia T, Hoek EMV, Somasundaran P, Klaessig F, Castranova V, Thompson M: Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater 2009, 8:543–557. 29. Pan Y, Neuss S, Leifert A, Fischler M, Wen F, Simon U, Schmid G, Brandau W, Jahnen-Dechent W: Size-Dependent Cytotoxicity of Gold Nanoparticles. Small 2007, 3:1941–1949. 30. Rothen-Rutishauser B, Mühlfeld C, Blank F, Musso C, Gehr P: Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway model. Part Fibre Toxicol 2007, 4:9. 31. Xia T, Kovochich M, Liong M, Zink JI, Nel AE: Cationic Polystyrene Nanosphere Toxicity Depends on Cell-Specific Endocytic and Mitochondrial Injury Pathways. ACS Nano 2008, 2:85–96. 32. Unger RE, Krump-Konvalinkova V, Peters K, Kirkpatrick CJ: In Vitro Expression of the Endothelial Phenotype: Comparative Study of Primary Isolated Cells and Cell Lines, Including the Novel Cell Line HPMEC-ST1.6R. Microvascular Research 2002, 64:384–397. 33. Weksler BB, Subileau EA, Perrière N, Charneau P, Holloway K, Leveque M, Tricoire-Leignel H, Nicotra A, Bourdoulous S, Turowski P, et al: Blood–brain barrier-specific properties of a human adult brain endothelial cell line. The FASEB journal: official publication of the Federation of American Societies for Experimental Biology 2005, 19:1872–1874. |
URI: | http://wrap.warwick.ac.uk/id/eprint/49396 |
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It was easy, quick, simple, cheap and stylish. deffo I will choose Glasses Direct again
I had my old plastic frame and all I was looking for is reglass it. I had my eye test in specsaver and they said they can't do it because it is plastic (and because I'm not buying a new one from there) feeling so disappointed I talked with a friend who recommend your company. And, it was the best choice. Now I got my frame and my new glasses. Thank u.
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Entries in 'centresoft' (16)
MCV AWARDS 2013: CentreSoft wins Grand Prix
Distributor CentreSoft won the final honour of the night at the MCV Awards: the Grand Prix.
Celebrating 30 years of CentreSoft
MCV has put together a special supplement honouring three decades of the long-running UK distributor.
CentreSoft turns 30
CentreSoft is this week celebrating 30 years of trading in the games industry.
Square Enix CEO and the death of consoles in this week's MCV
This week's MCV is now available to download - and it is jam-packed with great features, including an extensive interview with Square Enix CEO, Yoichi Wada.
SBK Generations
Retail Previews in UK RETAIL Sponsored by Gioteck
James Batchelor finds out why Milestone's latest is the biggest motorbike racer of 2012.
Five indies win Centresoft Vita challenge
Sony and CentreSoft have thanked UK independent retailers for their support during the launch of PlayStation Vita.
IN PICTURES: Retail dresses up for Batman launch
Everyone may be recovering from the Modern Warfare 3 launch party, but that's not the only game that saw retail go all out to draw in fans.
Distributors' Christmas opening hours
The following guide offers you the details on who is open when this Christmas. Print his page or click here to download a sexy PDF.
0121 506 9585
Gerry Berkley joins Centresoft
Retail veteran Gerry Berkley has joined Centresoft as consumer fulfilment sales manager.
Centresoft secures Best Buy Europe account
Best Buy Europe has strengthened its UK launch plans by appointing Centresoft to exclusively handle all gaming products, MCV can reveal.
Warner hails indie showcase
Warner and Centresoft have hailed their joint Independent Retail Showcase held in Birmingham on Wednesday August 5th 'an outright success.'
Centresoft snaps up Majesco account
Centresoft has signed an exclusive deal to become Majesco's distribution partner in the UK.
Centresoft signs Nordic Games distribution deal
Centresoft has signed an exclusive distribution agreement with Nordic Games.


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Be it enacted by the Senate and House of Representatives in General Court assembled, and by the authority of the same, as follows:
SECTION 1. The treasurer of the city of Woburn, with the approval of the mayor, is hereby authorized to borrow at one time or from time to time such sums of money as may be necessary, not to exceed an aggregate amount of one million one hundred and twenty-five thousand dollars, for the purpose of paying any judgment rendered against said city in the case currently pending in the United States District Court under Docket No. 95-12372 NG, and providing funds to make any required or agreed to indemnification payments to, or on behalf of, any city officials or employees with respect to any judgment rendered against such officials or employees or any settlement agreed to by said city with respect to the case currently pending in, or on appeal from, the Middlesex division of the superior court department of the trial court of the commonwealth under Docket No. 93-6797, and providing funds to make any settlement payments agreed to by said city with respect to such lawsuits. Notwithstanding the provisions of clause (11) of section seven of chapter forty-four of the General Laws or any other general or special law to the contrary, no further action by said city council shall be needed with respect to any such borrowing, each issue shall constitute a separate loan, each loan shall be payable within ten years from its date and indebtedness incurred under this act shall be included in determining the limit of indebtedness of said city under section ten of said chapter forty-four but, except as provided herein, shall otherwise be subject to the provisions of said chapter forty-four. Proceeds of any such borrowing may be expended without further authorization or appropriation by said city.
SECTION 2. This act shall take effect upon its passage.


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Plaid:Craft One Stroke Brush Set is ideal for the application of the one stoke painting technique which creates great florals and greens. Featuring (11) brushes with smooth wood handles that make them a breeze to work with, value pack contains (2) script liners - #1 and #2, seven flats - #2, #4, #6, #8, #10, #12 and 0.75-inch, and (2) scruffy - 0.25-inch and 0.75-inch. This product is made in USA.
This Web site is intended for use by US residents only. See International Sites. See our delivery policy for full details. Copyright 2013, Staples, Inc., All Rights Reserved.


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South African Freight Management on the NetBeans Platform
CoreFreight is a fully functional "operations support system" for Freight Forwarders and Customs Clearing operators.
The system provides full forwarding functionality from production of necessary transport documents, e.g., airway bills, to invoicing clients, and managing profitability on a shipment-by-shipment basis.
Statutory documentation and EDI messages for customs clearing in the South African environment is also provided.
NetBeans Platform
There were a couple of reasons that made us decide to use the NetBeans Platform as the foundation of our new application. Firstly, the NetBeans Platform is built on Swing, so all those UI libraries that you've used in the past can still be reused. Things like user notifications, update management and the modularity that is inherent to the NetBeans Platform are all things that we would've had to spend time implementing (and debugging…).
Additionally, the support for Maven NetBeans Platform applications is just getting better and better. Initially there were a couple of things that were very difficult to do relative to the Ant build process. However, now there is very little that cannot be accomplished using the Maven build process for NetBeans Platform applications.
(Note: Opinions expressed in this article and its replies are the opinions of their respective authors and not those of DZone, Inc.)


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Best afford. DSL for SF, Bay Area?
So I moved to this expensive city from Concord and I needed new internet. My last service was with Sonic and they were awesome but now moving here means I can't afford it. Does anyone know a good DSL service that's cheap and has reliable CS? but don't mention ATT.I used to be with ATT but they were a b*tch to deal with and I don't want to rely on the retention dept to help renewing my contract but if there's nothing else then I might have to go with it. Currently I'm debating if I should go with DSLX because they are offering 3MBps/512kup for $14.95 which is almost or the same as ATT U-verse. I don't know if I can renew the contract to keep the same price after the year ends? Also DSLX still requires a stupid landline service just to use it right? If so, then I need to check with them to see if they offer ULTS. Any of you SF'ers or Bay Area residents have good suggestions? Please don't suggest cable from comcast or whatever, because I just mentioned I moved here, and the rent is killing me over here. I just need basic internet to do research, but reliable enough to stream videos online and maybe playing a few games here and there online on my PS3 with my friends who residents in Cali without having lag. |
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Packers News: Charles Woodson signs with Raiders
After another lengthy stint on the free-agent market, former Packers defensive back Charles Woodson has signed a one-year contract to return to the Oakland Raiders, according to the team's official Twitter account.
Woodson was the No. 4 overall pick to the Raiders in the 1998 NFL Draft. He played eight years with Oakland but had no serious suitors when he became a free agent in 2005.
Late in the free-agent process, Woodson signed a seven-year contract to play in Green Bay where he would go on to play seven seasons. He was named NFL Defensive Player of the Year in 2009 and was a four-time All-Pro selection during his seven years with the Packers.
Versatility and instincts made Woodson a turnover machine at the peak of his career. His post-game speech, in which he urged the team to have "one mind, one goal, one purpose and one heart" after defeating the Chicago Bears in the 2010 NFC Championship Game, is referenced on the team's rings for Super Bowl XLV.
After originally suggesting he only wanted to play for a Super Bowl contendor, Woodson changed his tune as he remained unsigned. The Raiders won But now Woodson, 36, will return to Oakland to help shore up a secondary that allowed the fifth-most points in the NFL last season.
The Packers, meanwhile, will turn to Jerron McMillian and M.D. Jennings to fill Woodson's shoes safety. Both players saw time with the first-team defense Tuesday at the Packers first public OTA of the 2013 season.
Follow @MJEversoll
Marques is a Journalism student, serving as the Sports Editor of UW-Green Bay\'s campus newspaper The Fourth Estate and a Packers writer at Jersey Al\'s AllGBP.com. Follow Marques on Twitter @MJEversoll.


text: Originally Posted by zach619 Nice, glad to hear you all had a fun day. I can't wait to see some shots of CB and WB. I bet Atlantic Beach etc. had a good day too. Its good to hear that everyone was able to get out and give it a try today. I know a couple of days ago a lot of people were worried about that swell getting way outta control. Sometimes a couple feet over head is all you want. wind was all wrong for atlantic beach, it wasn't that great


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Ever wonder about the cool costumes and characters in the Star Wars Universe? Come learn how a few are done!
Overview of armor building; plastic types, adhesives, cutting and detailing.
Overview of latex mask making; product types, clay molds, detailing products.
Overview of Tusken Raider costuming; soft materials, construction methods, weathering.


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Cancer drug company DARA BioSciences reported a larger net loss last year as it ramped up sales and marketing efforts to support the Raleigh company's products.
DARA reported a net loss of $7.3 million, or 60 cents per share, compared to a net loss of $6.17 million, or $1.20 per share, in 2011.
As of the end of the year, DARA had cash and cash equivalents of nearly $6.5 million.
DARA raised about $2.5 million from private investors over the first three months of the year, money that the company expects to see it through 2013.
In June, DARA launched its first product, Bionect, a treatment for skin irritation and burns arising from radiation therapy. It followed up with the October launch of Soltamox, its liquid version of the popular breast cancer drug tamoxifen.


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This is just an idea for green.I'm sure your artwork is better..Milky green and silver ribbon. Plume head. Tour linen. Transparent stones / rocks, green / green crystal.
Jan 4, 2013, 07.04 PMby Mary Athey
Lovely.hairbands x
Jan 4, 2013, 07.10 PMby razieh khlili
tanksss.Kindness artists like you. Encouraged me.
Jan 4, 2013, 01.05 PMby razieh khlili
Jan 4, 2013, 12.32 PMby misumihaela
lovely ideea!
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Prospect.2 New Orleans BEATING HEART BIENNIAL by Emily Nathan. The Prospect.2 New Orleans biennial, overseen by curator Dan Cameron, brings 27 artists to 16 venues in the Big Easy., 11/02/2011
Willem de Kooning BEAUTY AND THE BEASTLY ARTIST: WILLEM DE KOONING'S DESTRUCTIVENESS by Donald Kuspit. Who is de Kooning's woman? An essay on the occasion of "De Kooning: A Retrospective at the Museum of Modern Art., 10/06/2011
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More resources: Higher Education, Career Development, and K-12 Education.
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DescriptionAfter about 25 feet, an ancient, manky bolt ladder leads to some thin nailing which then leads to some clean pro. There's a good stance under the final headwall crack. ProtectionStandard aid rack. Some spotters and lots of pads at the start would make decking less unpleasant.


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Vintage Christmas Backgrounder
Stamp size – 4.5 × 5.75 inches
Co-ordinating with the hugely popular Very Vintage Christmas and the original Vintage Ornaments sets, this densely patterned background stamp exudes vintage elegance!
All images and stamp designs are Copyright © 2012 Waltzingmouse Stamps, Claire Brennan.
Small images show cards made using these stamps, other stamps may also have been used on these samples.


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JACKSONVILLE, Fla. -- A famous Jacksonville landmark is back in its place.
The historic Jacob Jewelers clock has been reinstalled to its post on the corner of Laura and Adams streets downtown. Mayor Alvin Brown and other city leaders attended a ribbon-cutting ceremony today to dedicate it.
It has been away for extensive renovations. The clock dates back almost 80 years.
The re-installation began last month with the construction of a concrete foundation to house the 2.2-ton clock. The clock is one of 12 remaining out of only 100 made in 1901. Jacobs Jewelers gifted the clock to the city in 1995.
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Anencephaly (cont.)In this ArticleWhat is anencephaly?Anencephaly is a condition that prevents the normal development of the brain and the bones of the skull. This condition results when a structure called the neural tube fails to close during the first few weeks of embryonic development. The neural tube is a layer of cells that ultimately develops into the brain and spinal cord. Because anencephaly is caused by abnormalities of the neural tube, it is classified as a neural tube defect (NTD). What are the causes, signs, and symptoms of anencephaly?If the neural tube fails to close properly, the developing brain and spinal cord are exposed to the amniotic fluid that surrounds the fetus. This exposure causes the nervous system tissue to break down (degenerate). As a result, people with anencephaly are missing large parts of the brain called the cerebrum and cerebellum. These brain regions are necessary for thinking, hearing, vision, emotion, and coordinating movement. The bones of the skull are also missing or incompletely formed. Almost all babies with anencephaly die before birth or within a few hours or days after birth. How common is anencephaly?Anencephaly is one of the most common types of neural tube defect, affecting about 1 in 1,000 pregnancies. However, most of these pregnancies end in miscarriage, so the prevalence of this condition in newborns is much lower. An estimated 1 in 10,000 infants in the United States is born with anencephaly. Medically Reviewed by a Doctor on 10/23/2012 Patient CommentsViewers share their comments
Anencephaly - Causes
Question: Do you know what causes anencephaly?


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Results 1 to 7 of 7
Thread: Feedback from subwoofer
Feedback from subwoofer
I have 3 12inch kicker L5's with a 1200.1 Kicker amp in a 2004 Tahoe. I am getting alot of feedback it almost sounds like the exhaust coming through the subs while sitting at a stop, but as soon as I take off it goes away. I thought it was the ground at first but I cut a small piece of carpet up and sanded down to bare metal and grounded there. Any suggestions?
2004 chevy tahoe 35% tint on front windows, 5% tint on the back half ,flowmaster exhaust dumped, Spectre 9900 CAI, 3 12 inch kicker L5's, in-dash DVD/Navigation,kicker 1200.1 amp, removed cargo rack, black headlights lense, black turn signal lense, smoked tail lights, 8000K HID lights.
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- Oct 2010
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- Mckinney, Tx.
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Upgrade your RCA cable!
10-27-2010, 10:36 AM #3I have tried that once before but did not make it ANY better at all
2004 chevy tahoe 35% tint on front windows, 5% tint on the back half ,flowmaster exhaust dumped, Spectre 9900 CAI, 3 12 inch kicker L5's, in-dash DVD/Navigation,kicker 1200.1 amp, removed cargo rack, black headlights lense, black turn signal lense, smoked tail lights, 8000K HID lights.
10-27-2010, 10:46 AM #4make sure your ground from amp to frame is less then one foot. Any longer you will have issues with grounding. If the humming goes away with acceleration, it is more then likely engine noise. You have interference somewhere. Double check all your connections. Any bare wires running next to power leads should be shielded.
10-31-2010, 10:21 PM #5I am new to sound systems but one thing I read many times was not to run your power cable next to the RCA cables. I did mine on opposite sides and it sounds great. Don't know if that's your problem but it could. Hope this helps!
'01 Chevrolet S10
-custom air intake
-painted dash
-footwell lights
-Strange Performance stickers
-exhaust coming soon
-Xplod Head Unit with iPod connectivity
-12" Sony Xplod woofer
-Majestic 400 watt amp, bridged
If at first you don't succeed, then skydiving is not for you.
10-31-2010, 10:24 PM #6Yes, running the RCA cables next to the power or remote wire will possibly cause feedback. You also may want to run more than one ground wire. I haven't been in a situation where I needed to do that personally but I have read threads where guys have had to do that.
1999 Chevrolet Suburban LS K1500
1991 Chevrolet S10 4.3L 4x4
2005 Suzuki Forenza (GM/Daewoo)
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- Oct 2010
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- 01832 Massachusetts
- Posts
- 85
Agreed with the above... I use to do custom installs professionally. One of the first things that was taught to me was run power alone on one side of vehicle and the rca on the other side. Remote power would not cause road noise if that were ran with the rca's... I am willing to bet that those rca's are near some high voltage
2010 Crew Cab Silverado LT
Rough Country level kit
Kenwood DNX5160 (In dash NAV/DVD)
Kenwood KAC - 8105D (Mono Amp)
JL Audio 10w3
Rhino lined
Rugged Cover
Undercoated rear wheel wells
Fully De-badged (except grill)
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text: Abbreviation: HOR Accessible DirectionsThis building is accessible via access through Derrick Hall and Math Computer Science. From the Quad, follow the ramp to the first floor of Derrick Hall. Enter Derrick Hall (semi-automatic door) and take the elevator to the Mezzanine. Exit the rear (west) entrance and turn right (north) on the sidewalk. Enter the south entrance of Math Computer Science and take the elevator to the 3rd floor. Exit the building and cross Pickard Street to the east side of Smith Drive. Hornsby Hall is located on the east side of Smith Drive. Accessible ParkingAccessible parking is located in front of the building. Accessible EntranceThe front entrance of the building is accessible. There is not a semi-automatic door. | |


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You got that wrong, Speedo. I am not part of that silly modesty obsession. I just don't believe the CFNM versions of public-school swim classes of yore.<quoted text>
Pity you didn't have a female PE teacher. This should wake up tnmdude from his grave and give the MM/LL/cadad Modesty Crusade something to talk about.
Comments (Page 2)
To Zuiko aka Sir Arthur hello....Yes your statement above is a historical fact,The word gymnasium is a greek word meaning to go naked. |
Halifax, Canada |
Agreed- also- quite certain Zuiko is correct` about the male athletes performing in front of females as that would make them try their best to impress the fav lady. Gee- Zuiko knows a lot about everything !! |
To Coyote hello women from Sparta once took part in the Olympic games as horse riders-and yes they to were naked.
To Coyote hello i know a lot about everything as well,I know that your not circumcised-Katie Mellish xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxx. |
Halifax, Canada |
U R correct as always-(well not ALWAYS, but usually). Actually those gals from Sparta were a pretty independent bunch and as we know were very athletic. I recall reading that the women held their own games in a different location and their events were nude as well. At the men's games I think the only females permitted were sorceress' and prostitutes. I think Sir A(aka Zuiko) would have preferred to be a contestant at that locale whereas I would have attended the ladies' events- just to see the skill levels you understand.... I think there is a question of whether the first female participant actually drove the chariot or simply owned the horse(s).
Budapest, Hungary |
If you type "gym" at this school memories homepage there is a proof that there were hardly any schools where boys were allowed to wear shirts for PE.
http://www.friendsreunited.com/Search/Memorie... |
To Coyote hello yes you are right female charioteers did tale part in the Olympic games the women who took part were from Sparta. |
Halifax, Canada |
I would not be so sure Katie-- it is/was suggested m'lady (Spartan society lady with athletic abilities) simply owned the horse and had the chariot piloted by a male slave. It is` clear the only females present at the men games were sorceress' and prostitutes so the spartan ladies held their events at a different location..only proper U understand....
To Coyote hello you maybe right in what you have posted here but i will try and prove you wrong-you maybe right here but lets see if i can find evidence to prove me right.All the best Katie Mellish xxxxxxxx. |
Halifax, Canada |
U cannot do it my friend- but try if u will ! Zuiko knows !!.... he @ u know it all 4 sure !! xxxx
To Coyote hello i bet you will not say no to a Kipper breakfast and a fish supper with me-Katie Mellish xxxxxxxx. |
Halifax, Canada |
U R correct once again kid ! |
Oswestry, UK |
Coyote, in the ancient greek times the opposite sex were not allowed in. So unimformed, and yet you expect us to believe your weird fetishes. |
Oswestry, UK |
I wonder what it is about nude boys sliding across the wet shower floors Bob? |
Halifax, Canada |
Hey Kip- thanx for the misinformation-duh- what is unimformed ? U read the saga of the early Olympics and U will discover sorceress' and prost... were able to attend !!! |
Chester, UK |
Yeah, right, I wouldn't believe you coyote. |
Since: Jan 13 1,446 Location hidden |
Judged: 1 largelout is misinformed, as usual. The Olympics were an exception and only held once every 4 years. Meanwhile there were stadiums all across Greece in nearly every town, and later all across the Roman Empire, where nude male athletic events were held regularly and attended by everyone, including female spectators. In fact parents encouraged their daughters to attend so they could pick a future husband from the athletes. The young male athletes were also proud to display themselves naked in front of the female spectators, and even sometimes offered their trophies like garlands won in each event to the girl of their choice among the spectators. But LL is too ignorant and uncultured to know about these things. |
Chester, UK |
Judged: 1 1 I have read about it, females were forbidden to go to the stadiums. |
And no females permitted at the Greek olympics either
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Rented the whole bottom floor for a candlelight dinner
from Kanye West – White Dress Lyrics on Rap Genius
In an attempt to salvage what's left after some emotional turbulence and to repair their relationship, Ye rents out the whole bottom floor of a restaurant for a sweet candlelight dinner!
And we all know Ye will drop stacks for his boo, like when he rented out the AT&T stadium to propose to Kim.
To help improve the meaning of these lyrics, visit "White Dress" by Kanye West Lyrics and leave a comment on the lyrics box


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Bermuda: Resources
Main Government Page |
gov.bm |
Statistics Office |
govsubportal.com |
Central Banks |
bma.bm |
Stock Exchanges |
bsx.com |
News Sites
Bermuda: The Royal Gazette
The Royal Gazette is daily publication covering news in Bermuda. A wide variety of topics are covered, including business, sports, and community issues.
Regulatory Agency Sites
Bermuda Monetary Authority
The Bermuda Monetary Authority (BMA) is the regulator of the Financial Services Industry in Bermuda. This site contains all of the information on regulations and compliance sorted by sector.
Other Resources
Bermuda: The Bermuda Chamber of Commerce
The Bermuda Chamber of Commerce is a non-profit, apolitical organization established to advance the commercial, industrial, civic, trade and general interests of Bermuda. The sites contains tourist information, e-business opportunities, and hospitality resources.


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LG TV and AMD Mobility Radeon HD7850M via HDMI sleep problem
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snapcase07 |
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so what? sarah silverman is a dog-lover. she is still the funniest woman in comedy, and possibly,… -
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Britney's Trip from Trashy to Illiterate
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text:
Manhattan Research offers one-page snapshots and Infographics of eHealth and digital pharma data and insights from the latest consumer and healthcare professional market research: Mobile Health Trends for 2012 This snapshot previews data from the Cybercitizen Health® study of how mobile health will bring about real healthcare changes by motivating behaviors and enabling on-going care and tracking. Nurse Practitioner and Physician Assistant This snapshot highlights key trends among NPs and PAs. Digital Doctors Global Dashboard This complimentary Digital Doctors Global Dashboard shows a snapshot of data from the Taking the Pulse® Global Series. Manhattan Research, a Decision Resources Group company, is a global pharmaceutical and healthcare market research and strategic advisory firm. We conduct annual research studies covering digital health trends among healthcare professionals and consumers in the Americas, Europe, and Asia. Broad consumer and physician research is complemented by targeted analysis among target consumer therapeutic and healthcare professional specialist segments.
For more information about how Manhattan Research can help you combine in-depth, trended data with insights from our healthcare analyst team to shape better informed marketing and sales strategies, contact email@example.com or call 1.888.680.0800, ext 2.
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St. Bonaventure 76, Fordham 65: Box score analysis and where does Fordham go from here?
BRONX, NY – On Wednesday night, St. Bonaventure traveled to Rose Hill Gym and earned a 76-65 victory over Fordham. It was a battle of two teams trying to get to the middle of the pack in the Atlantic 10, entering the night just a game apart from each other in the standings.
We start by taking a look at some of the key numbers on the evening, then some notes that expand on them:


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I didn't ask for this
My name is dick smart, To learn more about me, go to my facebook or website.
Please help my family, we're about to lose everything. Any money is well appreciated.
This is CRAY-1.6.. One of the first supercomputers.
In Code Lyoko, the supercomputer is modeled after the CRAY-1.6
DUDE CRAY HAS ONE OF IT'S OFFICES LIKE TWO BLOCK FROM MY HOUSE! On a more related note, I should watch Code Lyoko again,...


text:
Orlando's Hip Hop Live Online 24/7
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You go, Nelly!! You may have heard about Amanda Berry. She's one of the three young women who were kidnapped, held captive and tortured in the Cleveland area for about a DECADE before finally being rescued earlier this year. Well, she was introduced at a music festival in Cleveland over the weekend, and later she got to share the stage with Nelly, who praised her strength and courage.
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We're sorry, Charles Rennie Mackintosh Coaster is no longer available
Why not try one of these instead?
Made in Britain
This coaster is a Gillian Kyle tribute to Charles Rennie Mackintosh - Scotland's renowned architect, designer and super 'tache!
This design is from the 'Local Heroes' range by Gillian Kyle in collaboration with young illustrator Clare Forrest; celebrating colourful characters past & present. Finally you can have a cup of coffee with Robert Burns or spend some quality time drying the dishes with Charles Rennie Mackintosh in your own kitchen! The other characters available in the range are listed below.
'Local Heroes' includes: Robert Burns, Charles Rennie Mackintosh, Mary Queen of Scots, Bonnie Prince Charlie, Alexander Graham Bell and Burke & Hare!
made from:
Individual coaster (also available in a mixed set of 6) decorated with our charming Charles Rennie Mackintosh design. Made entirely in bonny Scotland, these melamine coasters are finished with a felt backing.
Each coaster measures 110mm x 110mm and 3.2mm in thickness.
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text:
Title screen
Intro: Aika
Intro: Vyse
Intro: assorted bad guys
Intro: Fina
Pirate Island, near a save point
Status screen (Spanish version)
First-person perspective makes the game world even more immersive. Check out the view from high above
Valuan ship. Note the "steampunk" design
Regular battle. Targeting enemy
"We won, we won!" :)
Vyse enjoys awesome graphical effects while climbing the ladder
Boss battle. Vyse attacks!
Aika heals Vyse
Vyse executes one of his special moves
Pirate ship interior. Note the detailed graphics
Ship navigation. Ahh, the adventure...
Now *that's* how you make an entrance!
You'll sometimes be able to make dialogue choices.
Arcadia consists of floating islands with the skies as their seas.
Shop interface.
The mysterious girl central to the adventure.
Roaming around a friendly town.


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Current News
The Spring 2014 Housing Application is now available. You can apply online today by logging in with your Net ID and Password. Apply Now!
The Intent to Vacate Form is now available to students that are seeking to be released from their housing agreement due to graduation. Please submit the completed Intent to Vacate Form along with all supporting documentation by December 1st, 2013 to be considered.
All first year undergraduate and incoming transfer students as well as newly admitted graduate students are required to have the meningococcal vaccination to be eligible to live in University Housing. Please read the Rutgers University Immunization Letter for more information about required...


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What Are The Complications Of Genital Warts Hpv
Here's a taste of what TheBody.com has to offer on this topic:
Get the Facts
Human Papillomavirus (HPV)What Is HPV? How Can HPV Be Detected? Can HPV Infection Be Prevented? How Are HPV Infections Treated? The Bottom Line NOTE: In the U.S., counseling and referrals are available on a national human papillomavirus (HPV) hotline. Call toll-free at 877-HPV-5868 (877-478-5868). What Is HPV? There are over 100 viruses known as human papilloma virus (HPV). They are common. One study found HPV in 77% of HIV-positive women. HPV is transmitted...
Ask the Experts
genital warts (HPV, 2011) (HUMAN PAPILLOMA VIRUS AND HIV/AIDS, 2011)if a person have genital warts does it mean that he/she is going to have a positive syphilis test like VDRL or RPR?...Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation
Hi,No. Genital warts are caused by a virus, the human papilloma virus (HPV). Syphilis is caused by a bacteria, Treponema pallidum. You can read much more about HPV and syphilis in the archives of this forum. I'll repost below a sample of what...
Read more »
Genital warts (HPV) and HIVHi Dr Bob,Thanks for all you hard work and answering the questions of all worried people.Here are the delima i am goin throuh. Last year Dec 05 engage with the safer sexual with a sex worker. Condom was properly used and i checked at the end it was...Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation
Hi,If the latex condom was used properly and did not fail (break), your HIV-acquisition risk would be essentially nonexistent.The cause of genital warts is HPV, human papilloma virus. Unlike HIV, HPV can be transmitted by mere skin-to-skin contact....
Read more »
hpv and genital wartswhen someone has hpv or genital warts, do they have to take medication to supress having out-breaks?...Response from Ryan M. Kull, C.S.W
Columbia University's Health & Related Services
I am not aware of any medications that are effective in suppressing outbreaks of genital warts. Warts should be treated when they are present. Aggressively treating the warts may reduce the chances of warts coming back, but it is not guaranteed....
Read more »
Hpv/genital wartsHello, Is hpv genital warts forever and can they under your buttcheek or do they most likely occur inside vagina. Will I have it forever? And will I always pass it to other sexual partners? ...Response from Richard Cordova III
Test Positive Aware Network
Hi, there:You can get genital warts anywhere in the boxer-short region. Imagine putting on a pair of boxer shorts. Anywhere that the boxer-shorts covers is fair game.You can have the warts removed, and the body is usually able to clear the virus...
Read more »
Connect With Others
HPV/Genital Warts
Posted by Anonymous, 2 Replies
Hey, Whats up? My girl friend whom I've been with for a year now, recently got a pap smear done and she came up positive for HPV (abnormal cells). Me myself had been tested for STD's and a Visual Check up for HPV and in all tests I came up negative....
Read more »
help....Hiv and genital warts
Posted by Anonymous, 7 Replies
Hi everbody my name is john, 25 and need some help,,, i found out i had hiv about 7 months ago and at the same time was told i had genital warts, which at the time did not know what was the hardest to deal with in a funny kind of way, i know the hiv...
Read more »
genital warts
Posted by Anonymous, 4 Replies
i was told that you can have hpv genital warts for years and never have outbreaks. and years later break out and that it doesn't mean your boyfriend has cheated. is this true? Read more » do genital warts cause cancer in men
Posted by team1, 1 Reply
they do cause cervical cancer in women among other things. but its been stated that the warts are harmless to men? is that true?
Read more »
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06 Jan 2010 | #1 |
| Upgrade issues I recently upgraded my laptop to Windows 7, with an upgrade CD from my manufacture. I'm haveing problems with my system not coming out of sleep mode and not restarting. I'm having to reset and start in safe mode to get it the restart. |
My System Specs |
. |
06 Jan 2010 | #2 |
| Start over and do a clean install, it can be done with that DVD. If you are interested post back and we will tell you how it is done. |
My System Specs |
06 Jan 2010 | #3 |
| Please do, I'm not sure how to start over. Any help would be appreciated! |
My System Specs |
. |
06 Jan 2010 | #4 |
| Go to BIOS, you can find out how by reading your computer manual. It is done by striking one of the f keys (ususlly 2 or 12) repeatedly during boot. In BIOS change the booting order from Hard Drive to CD/DVD. Turn off your computer, put DVD in drive and boot up. Follow instructions on the screen. When you get to the screen asking for custom or upgrade, choose the advanced option. Format in that option, then choose custom install. Do not give your key number until you are all done. Make sure you uncheck the box that asks to activate automatically. When done, install your programs. Then try if for up to 30 days. If satisfied, then activate. Note, You have to reinstall all of your prgrams and you must save everything else like documents etc. You can make it easy by using windows easy transfer, found on the MS site. Do not forget to back everything up before you start. We never know what will happen. Good luck |
My System Specs |
06 Jan 2010 | #5 |
| Just take a look at this thread Clean Install with a Upgrade Windows 7 Version |
My System Specs |
06 Jan 2010 | #6 |
| Thanks for the advice, does it matter that my upgrade DVD is from the Manufacture of the laptop. Is this upgrade DVD a full version of Windows 7? Will what you are suggesting work with this DVD |
My System Specs |
06 Jan 2010 | #7 |
| I think yes, but to be sure you have no problems. Download the free Paragon software or use System Image which is part of Windows 7 and make an image on a separate hard drive. Anything goes wrong just restore and you are as good as you are now. http://www.paragon-software.com/home/db-express/ |
My System Specs |
06 Jan 2010 | #8 |
| Did your Upgrade kit come with a DVD clean copy of Windows 7, and a drivers/apps CD? If so, then you can boot the DVD as given, select Custom install, use Advanced drive tools to delete all partitions, create New one(s) as you wish, then format before installing to first partition. The installer is mostly driver-complete, with more coming on on Windows Updates after install. Any drivers then missing in Device Manager can be loaded from your Drivers/Apps disk, along with any favorite apps. Others can be found on the manufacturer's Support Download page for your computer model. |
My System Specs |
07 Jan 2010 | #9 |
| The CD i received is from ACER. It came with one with driver updates plus a Windows 7 DVD. How can I tell if this is a full version? |
My System Specs |
07 Jan 2010 | #10 |
| Make a system image of what you have, put it on an external hard drive. Then try to restore with the DVD. If things go bad, just restore from the image that you had. You cannot lose. |
My System Specs |
Thread Tools | |
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To display the associations of a class correct, a corresponding parameter in a Constructor and the assignment to the member variable is needed. E.g. for a association from Employee --> PaymentMethod
public class Employee {
private PaymentMethod paymentMethod;
/** green */
public Employee(PaymentMethod paymentMethod2) {
paymentMethod = paymentMethod2;
a assignment like
public Employee(PaymentMethod paymentMethod) {
this.paymentMethod = paymentMethod;
doesn't work! Because a constructor is not the only possibility to set a member variable, e.g. dependency injection like Spring, this is a real limitation!
Dan Padgett
Hi there
This has actually been changed in CVS a couple months ago so that the user is able to choose whether he wants to use association as green currently has it defined or the way you are proposing. You will probably be able to see these changes in the next release cycle.
Thanks for your interest!
Dan Padgett


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Girls Gone Tri--Fall Session
Bluffton/Beaufort County Pool
Sat 09/28/2013
All Womens, Sprint, USAT Sanctioned
100 Participants
Date | Time | Race | Distance |
Sat 09/28/2013 | 05:00 AM | Girls Gone Tri Fall Session--Individual | |
Sat 09/28/2013 | 05:00 AM | Girls Gone Tri Fall Session (Relay) |
All participants will receive race shirts. Awards will go to overall and age group winners. All finishers will receive a finishers award! This event is sure to please the greenest of athletes and those who are road tested. You don't want to miss it!
- Address:55 Pritchard Street
Bluffton, SC 29910


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IFA designation (source: National Association of Independent Fee Appraisers - http://www.naifa.com/member/require/index.html )
IFA - Member is a designation conferred to a residential appraisal specialist who is a Member of the National Association of Independent Fee Appraisers (NAIFA). NAIFA was founded in 1961 as a nonprofit professional association of real estate appraisers. Today, NAIFA has chapters throughout the United States, Canada, and Mexico. The Association's objectives are to raise the standards of the appraisal profession, gain recognition for its members as qualified professionals, and to promote fellowship among appraisers. There are seven [7] levels of membership with four [4] professional designations plus two [2] designation programs for designated members of other associations that sponsor The Appraisal Foundation.
IFA designates must be a member (Candidate, Associate, or Affiliate) of NAIFA and must meet the following requirements for IFA - Member status:
Experience: Receive credit for 2,500 hours of appraisal experience in no less than two [2] years.
Demonstration Report: Submit an original and one copy of a Self-Contained Appraisal Report that communicates a Complete Appraisal of a one [1] to four [4] unit residential property. Also submit an Experience Rating Form (ERF), which must be approved by the National Admissions Committee. Because of its rigorous education and experience requirements and commitment to adhere to strict industry standards and a professional code of ethics, you can be confident an appraiser with an IFA - Member designation will provide you with the very highest quality residential appraisal service.


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This article was originally distributed via PRWeb. PRWeb, WorldNow and this Site make no warranties or representations in connection therewith.
Sonetics Corporation redefines wireless team communication with new initiative and restructured website for improved customer experience.
Portland, OR (PRWEB) February 05, 2014
Sonetics Corporation, a leading global supplier of wireless headsets and communication systems, today announced its Discover Your Solution initiative. The initiative involves a major restructuring and optimization of its website, http://www.soneticscorp.com, which features customer-focused improvements in navigation and product search, an expanded array of safety and productivity resources, and makes it even easier for users to find the best team communication system for their needs.
Discover your solution expands our dialogue with our customers, said Julie Steding, marketing manager for Sonetics. Our customers asked us for a tailored user experience on soneticscorp.com. We were pleased to develop this new industry-specific look at Sonetics communication products with their input.
As part of the initiative, http://www.soneticscorp.com has been significantly restructured around specific markets, allowing users to focus immediately on the communication needs unique to their industry. Users will find a wealth of additional industry-specific resources, including videos, white papers, case studies, safety and productivity information, and a list of upcoming conferences and trade shows.
Most of our competitors focus on their products, Steding said. We believe it is more useful and productive to focus on specific industries, and how better communication can help work teams within those industries stay safer and accomplish more in less time.
Sonetics designs and manufactures wireless headsets and communication systems for a wide variety of applications, including aviation, construction, directional drilling, first responders, manufacturing, marine, towing and recovery, tree care, and general work teams. Wireless headsets significantly reduce background noise and allow all team members to communicate clearly and easily. Sonetics headsets fit over the ear for hearing protection and are outfitted with a boom microphone and ear speakers to allow hands-free voice transmission and reception through a mobile base station. Systems can also be connected to a two-way radio, allowing communication with remote users.
Studies in occupational health and safety have consistently shown that most workplace accidents are at least partially caused by poor or non-existent communication, said Simon Broadley, vice president of engineering. Wireless headsets not only create a safer work environment; in our experience they also keep team members focused and reduce down time. Continuous and instantaneous communication goes a long way toward making teams more cohesive and productive, he added.
Twice-named by Inc. magazine as one of Americas 500 fastest-growing private companies, Sonetics, together with its Firecom and Flightcom divisions, helps more than 500,000 customers in 90 countries hear and be heard under challenging circumstances. Visit http://www.soneticscorp.com/discover or call 800-833-4558 for more information.
For the original version on PRWeb visit: http://www.prweb.com/releases/2014/02/prweb11550538.htm
Information contained on this page is provided by an independent third-party content provider. WorldNow and this Station make no warranties or representations in connection therewith. If you have any questions or comments about this page please contact email@example.com.
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This website is best viewed in a browser that supports web standards.
Skip to content or, if you would rather, Skip to navigation.
ATCHISON COUNTY, MO (2010-08-27) Dove season kicks off the fall hunting season in Missouri, and the department of conservation is reminding hunters of the restrictions.
Dove hunters under 16 years of age would not have to get a permint. Those 16 to 64 years old will have to get two for the season. Atchison county conservation agent Eric Abbot says it's a popular season to Prepare for other foul shooting later in the fall. You can contact your local agent for more on the requirements this dove season.
© Copyright, KXCV


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Merry Christmas to all.....
Your guest have giving us such a wonderful year we would like to "THANK THEM"
For the month of December from 1st to the 29th, if you come any 2 days the second day if "FREE" that is right the second day is on us.... the last 2 day of December were not included because we are already full for New Years.
The rest of the month is open so give us a call an visit the Saugatuck shops for that very special gift and don't forget a Gift Certificate is also a very good thing for those who you just don't know who to by for.
My we all have a wonderful , Happy and Safe 2014.
Paulette Clouse, owner.
Variety of deals while you explore local art
Play in clay with your friends or loved one!
Holland Tulip Time & More
2013 Holiday Specials
Savings - Savings - Savings
"Looking for a Deal"
Stay ALL 5 days
Melt Your Stress Away
Hearts & Flowers in the Snow
Sailing Lake Michigan
Wine Lovers Getaways
Perfect gift for a beer lover
New this year to Saugatuck, so give them a try... Captain AL has been sailing all his life and would love to spend the day with you. Go to Fishing & Boating. Just let know when you would like to sail and it will be added to the cost of your room.
Live play's Down town Holland Michigan Trap for a Lonely Man Jan 30- Feb 1, 7-8, 13-15 at 7:30pm Sunday, Feb 9 at 2pm Tickets $16.00 -Adult / $14.00-Seniors / $10-Students Group rates available Specials!Open Night HCT Members Special - 2 for 1
Cross country ski and snowshoe rentals, and 5 different area trails to spend the day on. Saugatuck land Sharks has daily rentals for $14.00 per day for adults and $10.00 per day for children Store hours 10am -6pm
2 day stay in April and receive a $50.00 Gift Card to eat at the "Salt of the Earth"


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Let's get together guys...it's about time...I have a couple of things in mind that I would like to discuss to expand our group and activities.
Let's get together guys...it's about time...I have a couple of things in mind that I would like to discuss to expand our group and activities.
Sure am gonna miss you guys. Will def stop by the garage when I'm coming through lubbock again though.
KStyle, when are you planning on making a trip?
D@n, what did you have in mind, intrigued!
Why don't you have a build thread? MUST...HAVE...CARPORN...
Let's do it...I'm taking a 2012 Golf R to the next get together.
Are you guys aware of the Coffee & Cars happening in Lubbock every month? If you are not....the first Saturday of each month except on holidays there's a get together @ the Bistro on 98th & slide. If any of you guys wanna join and represent V-Dub hit me up and we'll go together.....it's from 8-10am.
It's Tuesday, you guys meeting? In town with a convertible mustang rental :p
Sent from my iPhone using Tapatalk
hey sent a request on the FB page dont think ive gotten accepted yet.. just picked up a 2013 CW GTI autobahn.. got back in to VWs after about 4 yrs of trading in my mk4.. there are acouple of us here in clovis... not many though..
Who wants to go to The Formula 1 @ Austin this weekend? I have two tickets for the Friday practice and Sunday race at a discounted price. These tickets are sold out and they are the best ones that you can buy! I'm willing to sell both tickets for both days of just for the race.
The hairpin #15 turn is the most dramatic on the track and will offer the most spectacular show. These are exclusive tickets and have access to some of VIP areas of the track.
Wow! Too bad I had to leave early...we have got to make it a tradition of showing up to the coffee & cars get together... 8+ cool V-dubs!
Tomorrow Coffee & Cars....don't miss out!
Tomorrow Coffee & Cars @ The Bistro!
Stolen in town...please send tips if you see it on the road.
OMG.. That's awful! Hope they find it soon
I'm a mechanical engineer, not a mechanic. I know why it works, not how.
Just FYI to the VW/Audi owners in Lubbock. If you don't know already, some of your favorite mechanics has left the VW dealer garage and started their own. It's called J Dee's Import Garage located in the Depot District, 1602 Ave F, 744-2444.
Rumor has it they are trying to become a APR Turning dealer in the future...
Just a reminder that the Lemans race is happening in September @ COTA in Austin! Click and join! Great seats, people, track day!
Last edited by d@n; 08-09-2013 at 09:44 PM.


text:
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text:
In the quiet time of evening
When the stars assume their patterns
And the day has made his journey
And we wondered just what happened
To the life we knew before the world changed
When not a thing I held was true
But you were kind to me and you reminded me
That the world is not my playground
There are other things that matter
And when a simple needs protecting
My illusions all would shatter
But you stayed in my corner
The only world I knew was upside down
And now the world and me, I know you carry me
You see the patterns in the big sky
Those constellations look like you and I
Just like the patterns in the big sky
We could be lost, we could refuse to try
But we made it through in the dark night
Would those lucky guys turn out to be
But that unusual blend of my funny friend and me
I'm not as clever as I thought I was
I'm not the boy I used to be because
You showed me something different
You showed me something pure
I always seemed so certain but I was really never sure
But you stayed and you called my name
When others would have walked out on a lousy game
And you could've made it through
But your funny friend and you.
You see the patterns in the big sky
Those constellations look like you and I
That tiny planet in a bigger guy
I don't know whether I should laugh or cry
Just like the patterns in the big sky
We'll be together â€~til the end this time
Don't know the answer or the reason why
We'll stick together till the day we die
If I had to do this all a second time
I won't complain or make a fuss
When the angels sing that that unlikely blend
Are those two funny friends, that's us
Writer(s): Gordon Matthew Sumner, David Hartley, David M. Harltley, David Harltley
Copyright: Wonderland Music Co. Inc., Artemis Muziekuitgeverij B.V.
Lyrics powered by www.musiXmatch.com
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text:
Victor H. Metcalf
This article needs additional citations for verification. (March 2013) |
Victor Howard Metcalf (October 10, 1853 – February 20, 1936) was an American politician; he served in President Theodore Roosevelt's cabinet an Secretary of Commerce and Labor, and then as Secretary of the Navy.
Born in Utica, New York, on October 10, 1853 to William and Sarah P. (Howard) Metcalf. He attended the Utica public schools, Utica Free Academy, and Russell's Military Institute at New Haven, Connecticut. In 1872, he entered Yale College where he was a member of the Delta Kappa Epsilon fraternity (Phi chapter); he left in his junior year to attend Yale Law School. He graduated in 1876 and was admitted to the Connecticut bar. In 1877, he continued his legal education at Hamilton College, and was admitted to the New York bar. He practiced in Utica in 1877, and then moved to Oakland, California in 1879. His law practice in California handled real property and commercial cases.
He married Emily Corinne Nicholson in 1881; they had two children.
He was elected as a Republican to the 56th, 57th and 58th United States Congresses, serving from 1899 until 1904. In congress he served on the Naval Affairs and the Ways and Means committees. Metcalf's legislation for reclamation of arid lands put him in touch with President Theodore Roosevelt.
President Roosevelt appointed him, on July 1, 1904, Secretary of Commerce and Labor. As Secretary of Commerce Roosevelt sent Metcalf to San Francisco in 1905 as an intermediator between the San Francisco school board and 91 Japanese students who were refused from entering public schools. A compromise was reached where the students would be permitted into the public schools while Japan would stop issuing passports to laborers.1 He served until December 12, 1906, when he was appointed Secretary of the Navy. During his term, he oversaw the world cruise of the Great White Fleet. The pressures of office took a toll on his health and he resigned as navy secretary November 13, 1908.
After leaving Roosevelt's Cabinet he returned to Oakland and resumed his practice of law, and engaged in the banking business. Little more than a month after his wife Emily died, Metcalf died in Oakland, February 20, 1936.
He is buried at Mountain View Cemetery, Oakland, Calif.
External links
- Victor H. Metcalf at the Biographical Directory of the United States Congress
- Men of Mark in America
- Biography
- The Political Graveyard
United States House of Representatives | ||
Preceded by Samuel G. Hilborn |
Member of the U.S. House of Representatives from California's 3rd congressional district 1899–1904 |
Succeeded by Joseph R. Knowland |
Political offices | ||
Preceded by George B. Cortelyou |
U.S. Secretary of Commerce and Labor Served under: Theodore Roosevelt 1904 – 1906 |
Succeeded by Oscar S. Straus |
Government offices | ||
Preceded by Charles J. Bonaparte |
United States Secretary of the Navy 1906–1908 |
Succeeded by Truman H. Newberry |
Victor H. Metcalf was a brother of the Delta Kappa Epsilon fraternity (Phi chapter).
HPTS - Area Progetti - Edu-Soft - JavaEdu - N.Saperi - Ass.Scuola.. - TS BCTV - TS VideoRes - TSODP - TRTWE | ||


text:
Hi all,
I'm going to be grinding some XP prior to the expansion on my Barb(s), and would like to group with some other WW Barbs if possible for the XP bonus and other MP benefits.
I do currently have one other who I will be farming with, and we would like a couple more similarly geared WW Barbs (250k+ unbuffed DPS at least) to party with. We will mostly be playing in the evenings (EU).
Let me know if you're interested. Without wanting to sound like an ass, we aren't too interested if your gear isn't up to par.
- 8/24/2013 7:01:26 PM posted a message on Posted in: Looking For Group
posted a message on Hit me up too OP, or anyone else in this thread.Posted in: Looking For Group
Gaskie#2632 -
posted a message on I love how all the people with 1 post are coming out in support. Not at all suspicious. Nosiree.Posted in: Diablo III General Discussion
posted a message on You'll also notice that none of those high DPS monks are anywhere near the top Paragon rankings. Dual wielding is clearly the way to go if you aren't blinded by large numbers.Posted in: Monk: The Inner Sanctuary
posted a message on Posted in: Monk: The Inner SanctuaryQuote from Lewbagger
Quote from Gaski
I like your approach to gearing and skills. A bit off the mainstream, very nice!
I like how you use two fist weapons, just as I guess Blizzard intended. I notice you don't have much LoH but quite a bit of Life Steal - how do you find life steal? Also - stacking FR, was that expensive?
Life steal is far superior once you're getting high enough crits, especially when there are lots of enemies around. It works better with AOE skills (like WoL, SW). As for stacking FR I don't think its much more expensive than any other resist.
Interesting - thank you. Maybe I'll try out some life steal since I'm regularly seeing 200k+ crits. -
posted a message on I have two Sun Keepers, a new one with 44% MF and a legacy one with 25% MF, I swap these in before elite kills for a total of 276% MF before NV stacks. It's really nice that you can use both even though they're unique equipped. Just a shame I wasted 13m on a second new Sun Keeper before I realised they were unique equipped.Posted in: Monk: The Inner Sanctuary
posted a message on Posted in: Monk: The Inner SanctuaryQuote from Obe
Quote from Gaski
I've never been a fan of items with really low stats and extremely high resistances (eg. your bracers, shoulders, etc.) but I guess considering your Inna's pants you kind of do need to find the resists elsewhere.
Other than that, I've always wondered why certain people upgrade the gems on their gear (read: excluding weapons and helm) to perfect/radiant stars when their gear is still relatively easy to upgrade - I mean I understand Christaras using full radiant emeralds because he has 2.5 billion gold but looking at your profile I see a bunch of pieces that could've been upgraded using the 40 million gold you spent on getting the perfect greens you have on your armor. Not that I think it's a bad choice, it just really confuses me because the gold spent:DPS gained ratio one has by upgrading gems past star is appallingly low compared to what it could be via upgrading actual gear.
I should also say that this is my totally unbuffed DPS. I'm at 85k with BoH.
One thing I should probably do like you is get fleet footed, 10% movement speed is quite great. Your gear's good overall too, that's for sure.
Mine: https://eu.battle.ne...460/hero/226352
The reason I have several "sub standard" items is because of the high MF and resistances on them. Bracers and shoulders being the best example. There is no chance that for anything less than a significant chunk of money, I could get items with super high dex/vit/AR/MF to replace them. As you say - I have to pick up resistsances somewhere and the tradeoff for that is slightly worse stats. Luckily for me the rest of my gear makes up for it. Sat in my bags I have several extremely good rings, bracers etc, it's just that I like to keep MF. I suspect DPS would be going over 100k if I were to go full DPS gear. I should probably test that.
Edit - Fleet Footed is something I only use Solo. It gives me a total of 32% run speed and is super nice. In groups I use the passive which increases damage when you heal party members. I find the DPS increase noticeable and significant. -
posted a message on Posted in: Monk: The Inner SanctuaryQuote from thundersteele
Quote from Swiftdeathx
Heres my build: http://us.battle.net...34/hero/1482989
I've opted to go for more spirit generation/ spending with the recent buffs and its working extremely well. (AOE/ Life steal with WoL is immense).
I like your approach to gearing and skills. A bit off the mainstream, very nice!
29m bid. I was a bit lucky, the auction ran out during that period when some people had problems logging in, so I saved a bit compared to the market price. That chest you have is nice btw
His DPS seems bugged, I was trying to compare it to my own gear and couldn't see how it was possible to have so much DPS, so I checked D3rawr and it's showing as 60k which is more realistic.
I like how you use two fist weapons, just as I guess Blizzard intended. I notice you don't have much LoH but quite a bit of Life Steal - how do you find life steal? Also - stacking FR, was that expensive?
Here's me - http://eu.battle.net/d3/en/profile/Gaskie-2632/hero/4578698 -
posted a message on Hi,Posted in: Looking For Group
I'm looking for a few people who might be interested in high MF Act 3 runs. My stats are:
1992 Dex
31% Crit
310% Crit Hit Damage
30k life
600 res all
900 Life on Hit
255% MF (With 0 stacks/Follower buffs) - Swapping out for kills.
Ideally I'd like a Barb/Monk to team with, or maybe even some sort of clan. Fire me a request in game if you think you might be interested - Gaskie#2632. -
posted a message on Hi mate,Posted in: Looking For Group
I'm looking for a melee partner for some Act 3 farming. I'm also a monk, with 51k DPS,
Feel free to add me if you want to give it a shot - Gaskie#2632 - To post a comment, please login or register a new account.


text:
By day, Nola Devlin (Poppy Montgomery) is an unassuming, frumpy magazine editor who is overlooked and teased by her coworkers. When the sun sets, though, and she is behind the glow and anonymity of her computer screen, she becomes the famous and "reclusive" advice columnist Belinda Apple. Nola's friends, tired of being overworked and overweight, band together to create the "Cinderella Pact," vowing to lose pounds by following the advice of their "fairy godmother," Apple. When her secret identity is threatened, Nola is forced to take her own alter ego's advice. But, as the group of friends drops dress sizes, their real issues are exposed, and better-than-expected life changes begin to blossom.
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Poppy Montgomery | Nola Devlin | |
Adam Kaufman | Chip/Alex | |
Michelle Harrison |
Writing |
Gary Harvey | Creative Producer |
Nancey Silvers | Writer |
Sarah Strohmeyer | Author |


text:
It's not everyday a MULTI-MILLIONAIRE takes time from his fishing trip to help a couple of stranded common folk ... but this weekend, San Diego Padres owner Jeff Moorad came to the rescue.
Moorad was hanging out at a secluded area of Lake Miramar in San Diego yesterday when a man approached him and asked for help ... because the battery in his car had died.
We're told Moorad -- who was watching baseball on his phone at the time -- sprang into action ... and helped the stranger get his car up and running again.
Unfortunately, the good karma didn't pay off for Moorad's team yesterday -- the Pads lost to the Cardinals 2 - 0.
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TMZ, you need to get your facts straight! The guy under the hood is JC Playford, a local bum in Ramona, CA. Google "JC Playford" to learn more about him. He DOES NOT drive an Escalade and this is probably his first time seeing one. He was probably under the hood of Moorad's Escalade, GET HIM AWAY FROM THE CAR!!!
TMZ - get the facts straight before you post dumb news.
that's JC Playford, a local bum in Ramona - taking a look at Moorad's Escalade... Boy how TMZ sure can spin things!!! Google "JC Playford" to learn more about the long haired fellow helping out the Padres owner. Get your story straight TMZ !!!


text:
Bed, Breakfast, and Marc Jacobs at the Elysian Hotel Chicago
June 30, 2010By: Mary Winston Nicklin
Now fashionistas can "get in bed with Marc Jacobs" with a special package offered by the Elysian Hotel, the luxurious new hotel in Chicago that landed a spot on Conde Nast Traveler's "Hot List" just after opening. Back in February, the Marc Jacobs Collection store launched in the hotel's street-level retail space causing quite the stir in Chitown (and nationally!) The "Bed, Breakfast, and Marc Jacobs" package, available through Labor Day, includes accommodations in an Elysian guestroom or suite, complimentary breakfast in bed, a Marc Jacobs beach tote, and a limited edition Marc Jacobs towel. Rates start at $415. As BagBunch puts it, "These Marc Jacobs accessories are just the thing for looking the part when sunning yourself near the Elysian pool."
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Posted in: Articles, Featured Luxury Hotels, Food and Wine, North America, Special Luxury Travel Offers, Hot Spots, Luxury Shopping, Fashion
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