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Definition of hoof in English:
Syllabification: hoofPronunciation: /ho͝of, ho͞of
( plural hoofs or hooves /ho͝ovz, ho͞ovz/)
- The horny part of the foot of an ungulate animal, especially a horse: there was a clatter of hoofs as a rider came up to themMore example sentences
- With their teeth, hooves, horns and dung, wildebeest have literally cultivated the grasslands.
- I closed my eyes, the horses hooves and the rocking of the carriage almost lulling me to sleep.
- She heard the clopping of horse hooves but she didn't know where it was coming from.
- 1 (hoof it) Go on foot: it was awfully hot, but we hoofed it all the way backMore example sentences
- If you are required to keep your cart on the path, you can end up walking farther than you would have if you hoofed it.
- Why go with a guide instead of hoofing it on your own?
- The bus wheezed up the road to the village of Naggar, where we disembarked, hoisted our packs, and started hoofing it.
- 1.1Dance: we hoof it reasonably fancily, and no one guffawsMore example sentences
- Fred's a smart alec sailor who bumps into his old flame while on shore leave, and it's not long before they're hoofing it to 'Let Yourself Go' and 'Dance'.
- But, no, he really does run a dance club, and Maria is soon hoofing it in Geneva.
- Certainly it's nothing new to see older dancers still hoofing it.
on the hoof
- 1(Of livestock) not yet slaughtered.More example sentences
- He said livestock was judged on the hoof at the show and subsequently slaughtered at the East London abattoir.
- This all seemed to work well, but as well as this, according to the regulations, you must have a vet to first inspect all slaughter animals on the hoof - thus adding to the expense.
- It is also used by a number of exporters in the Irish livestock industry who ship cattle on the hoof to Lebanon, Egypt and Europe.
- 2 • informal Without great thought or preparation: policy was made on the hoofMore example sentences
- Unfortunately they look set to continue the trend of setting parking policy on the hoof, in response to short-term financial pressures rather than in accordance with a long-term vision.
- But too often, he appears to be making up crime policy on the hoof, like his decision today to release hundreds of criminals early because the prisons are full.
- Are we about to witness more policy made on the hoof, or is this merely evidence they are struggling to defend the indefensible against valid widespread public protest?
- More example sentences
- If they're lucky, the couple will catch sight of the mousedeer, which is the world's smallest hoofed animal and features in local folklore.
- Foot-and-mouth is a highly contagious viral disease which causes blisters and fever in hoofed animals.
- Lions prey mostly on hoofed animals, although they occasionally consume fallen fruit.
Old English hōf, of Germanic origin; related to Dutch hoef and German Huf.
More definitions of hoof
Definition of hoof in:- The British & World English dictionary
- The English Synonyms


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Angel Statue
Set in Stone
In the solitude of a stormy morning, an angel casts a downward gaze in stony silence. Continue reading →
Angel in the Palouse – Statue in Colfax Cemetery
A second angel statue I found gracing the pioneer portion of the Colfax Cemetery in the heart of the Palouse. Continue reading →
Angel Statue in Colfax Cemetery
This beautiful marble statue of an angel graces the community cemetery on a hill over looking downtown Colfax, Continue reading →
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~I hope you enjoy your visit!
~Madge Bloom
- At the Controls - Rurality Blog Hop #51 posted on February 19, 2014
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- Coloring Outside the Lines: I'm excited too- can't wait for warmer weather and glorious sunshine.
- Catherine: We are still under a blanket of snow here in Southwest Saskatchewan. But there is forecast for double digets next week. Hopefully spring will come soon! Lovely shot! xo Catherine
- Contrasts – Rurality Blog Hop #53 (20)
- Wheat Manna – Rurality Blog Hop #52 (16)
- Teresa: Love those old buildings. There used to be so much artwork in the advertising/murals on them.
- Sheeps and Peeps Farm: We've seen a new one recently after an adjacent building was torn down. We've been trying to get a photo but there is a chain link fence obscuring much of the...
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- Teresa: I would never have known what it was, but it really does make for an appealing photo. Love the light in it.
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Rejoice...some sunshine


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Fashion Shoes 9:00 am Pre-Summer 2014 Maison Martin Margiela 22 Painted Derby Shoes Pre-Spring Summer 2014 is here. Doesn't time fly eh? Feels like we just got over post-Summer 2013 and present-Winter, well, I'm pretty sure that's still happening. Nevermind, a pair of shoes is a pair of shoes regardless of season. Now,… 2715 - Read more
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i'm just starting up with a web app using nitrogen and everything is going well. But i also want my app to interface with a riak db that i set up and i'm having some trouble.
I'm confused as to how I "include" the erlang client interface so that my code in nitrogen can access it.
I'm new to erlang and nitrogen, but i mean in general, for erlang, how do i include other libraries as reference? Do i just take the compiled beam files and throw it somewhere, then have an -include line at the top of my erlang code? if so, where do do i throw these files for nitrogen (it has its own separate erlang node instance i think)


text:
1 posts found | |
Elite Member
Joined: 1/13/10 EVE 2013 - DUST 514, CSM8, Fanfest, 10th Anniversary, Uprising, Odyssey, Valkyrie, Rubicon |
After Derek Smart's last blusterfest about how everyone else's game is rubbish/not as good/fail there seems to be complete silence on the GCO and LOD front. Any recent news on these? |


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Exclusive Extended Interview with Planned Parenthood's Cecile Richards
"We do more to prevent unintended pregnancy and the need for abortion than any organization in this country." – Cecile Richards
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Comedy Central's Indecision is the network's digital hub for news, politics and other jokes: we're here, we're everywhere. We're not affiliated with any television show. We're affiliated with ourselves.


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A Concise History of Canada
Part of Cambridge Concise Histories
- Author: Margaret Conrad
- Date Published: May 2012
- availability: In stock
- format: Paperback
- isbn: 9780521744430
$27.99 (G)Paperback
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Hardback, eBook
Looking for an examination copy?
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Margaret Conrad's history of Canada begins with a challenge to its readers. What is Canada? What makes up this diverse, complex, and often contested nation-state? What was its founding moment? And who are its people? Drawing on her many years of experience as a scholar, writer, and teacher of Canadian history, Conrad offers astute answers to these difficult questions. Beginning in Canada's deep past with the arrival of its Aboriginal peoples, she traces its history through the conquest by Europeans, the American Revolutionary War, and the industrialization of the nineteenth and twentieth centuries, to its prosperous present. As a social historian, Conrad emphasizes the peoples' history: the relationships between Aboriginal and settler, the French and the English, the Catholic and Protestant, and the rich and poor. She writes of the impact of disease, how women fared in the early colonies, and of the social transformations that took place after the Second World War as Canada began to assert itself as an independent nation. It is this grounded approach which drives the narrative and makes for compelling reading. In the last chapter, the author explains the social, economic, and political upheavals that have transformed the nation over the last three decades. Despite its successes and its popularity as a destination for immigrants from across the world, Canada remains a curiously reluctant player on the international stage. This intelligent, concise, and lucid book explains just why that is.
Read more- An engaging history of Canada by one of its most eminent historians
- The people of Canada, their forebears as well as the most recent arrivals, are at the heart of the narrative which favours social and cultural history
- For students and those with an interest in North America and its deep past
Reviews & endorsements
"This impressive history of Canada tells a rich and surprisingly new story in a relatively brief space. It is constructed on an awareness of the past half-century of historical writing and is especially convincing in its approach to Aboriginal communities, French-speaking households, and Atlantic Canada. I recommend it wholeheartedly." -Gerald Friesen, Distinguished Professor Emeritus, University of Manitoba
See more reviews"Conrad's book has energy and narrative drive and never seems didactic or heavy-handed. It has what Stephen Pyne calls the hallmark of all good non-fiction: "voice and vision." Instead of the literary conceits or clunky structural devices others may have relied on, Conrad trusts her own voice and the history of Canada itself to tell an inclusive yet coherent national story." -Jeffrey L. McNairn, Queen's University
"Margaret Conrad's elegant, erudite and sophisticated study of Canada's history is both dramatic in scale and detailed in exposition. Throughout, Conrad traces a clear narrative trajectory that juxtaposes Canada's internal social, political and cultural dynamics and its external influences, especially those emanating from the nation on the other side of Niagara Falls, to emphasize both the challenges and triumphs that the nation has faced in constructing and sustaining a distinctive, coherent and inclusive identity in the face of internal dissent, global unrest and economic uncertainty." -Susan-Mary Grant, Professor of American History, Newcastle University
"Recommended." -Choice
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Product details
- Date Published: May 2012
- format: Paperback
- isbn: 9780521744430
- length: 341 pages
- dimensions: 216 x 140 x 18 mm
- weight: 0.4kg
- contains: 54 b/w illus. 5 maps
- availability: In stock
Table of Contents
Introduction: a cautious country
1. Since time immemorial
2. Natives and newcomers, 1000–1661
3. New France, 1661–1763
4. A revolutionary age, 1763–1821
5. Transatlantic communities, 1815–1849
6. Coming together, 1850–1885
7. Making progress, 1885–1914
8. Hanging on, 1914–1945
9. Liberalism triumphant, 1945–1984
10. Interesting times, 1984–2010.


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Comfort Inn Near Grand Canyon is quite a large hotel in Williams. Simple, quality accommodation is offered with a 2-star rating.
Booking a room at Comfort Inn Near Grand Canyon gives you the benefit of free breakfast during your stay. Take a dive during your stay; there is an indoor swimming pool. Choose one of the 74 rooms. Free parking is another useful option when staying at Comfort Inn Near Grand Canyon.
The air-conditioning of Comfort Inn Near Grand Canyon keeps you cool on warmer days in Williams. From your room here, you can dial local numbers for free. Maintain your status updates via the rooms' wireless high-speed Internet access (complimentary). A coffee/tea maker is provided in the rooms of the hotel.
Show reviews
Check-in: | From 15:00 Check-out: Until 11:00 |
Internet: | Wireless high-speed Internet access (complimentary) |
Parking: | Parking (free) |
Rooms: | 74 |
General facilities: |
Swimming Facilities: |
Note: It is the responsibility of the hotel chain and/or the individual property to ensure the accuracy of the photos displayed. We are not responsible for any inaccuracies in the photos. | |
Please provide this reference number to our customer service center representative on request, so we can help you better


text:
Bacon Cheddar Chicken
A boneless skinless chicken breast recipe
Yield: 6 servings
Community Recipe from
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- shredded cheddar cheese
- bacon bits
- 6 boneless chicken breast
- Season the breast to liking.Butterfly the breasts.Coat inside with bacon bits,then stuff with the shredded cheese.Cook on hi broil for 9 minutes per flip,do 2 flips for a total of 27 minutes cook time.pull them out and coat top with bacon bits and cheese.Let stand 3 minutes and serve.
April 2012
This recipe is a personal recipe added by frank73300 and has not been tested or endorsed by MyRecipes.
Bacon Cheddar Chicken Recipe at a Glance
- COURSE: Main Dishes
- CONVENIENCE: 5 Ingredients or Less
- MAIN INGREDIENT: Poultry
- COOKING METHOD: Broil
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Spa Hotel Labe
Travel Blogs from Pec pod Snezkou
Czech Republic - Karlovy Vary
... kolonada).
Probably the most beautiful is the Mill Colonnade in the Neo-Renaissance style. It is 132 meters long, 13 meters wide and has 124 columns. It stretches over five hot springs.
The Hot Spring Colonnade is where you can not only drink the curative waters of the Vridlo (literally "Hot Spring", temperature 73�C/163�F), the best-known of the springs and symbol of Karlovy Vary, but also admire the 14-meter high fountain that ...
Into Germany
Today we drove towards the German border and visited one of the popular spa towns in Western Bohemia called Marianske Lazne. We then crossed the border late in the afternoon and spent the night in a campsite in Neustadt a.d. Waldnaab.
Dnes jsme jeli smerem k nemeckym hranicim a navstivili jsme jedno ze znamych lazenskych mest v zapadnich Cechach – Marianske Lazne. Pak jsme prekrocili hranice a stravili noc v kempu v Neustadt a.d. ...
80 Blasting to Cheb
... sic) out in the morning)
The place looks pretty so deserves some time. From here I will head further south into Slovenia and /or Croatia and promised myself to take it easy as today I have hammering it at a steady 130 km per hour most of the afternoon, as I needed to cover the 780 km distance before it was getting dark. Giving me an average of just over 100km per hour including three stops and some slow riding due to road ...
Karlovy Vary
... and a tower at the lookout which gives a 360 degree view of the surrounding scenery. The tower was built in 1914, and has a lift for those lazy people who don't want to climb the 150 stairs to the top. So bike riding in the morning, mountain climbing this afternoon (hmm, probably hill climbing, but who will contradict me!), as well as climbing the stairs of the tower, and then walking down to the town again ...
Dodging showers!
... water, waste pump out and a shower, and what would have been a lovely view over farmland, if we could have seen through the rain and mist. There was even a small castle that was floodlit at night!


text:
What's happening rap?
from Asher Roth – Turnip the Beet Lyrics on Rap Genius
Asher's first single after a short hiatus, "Turnip the Beet" addresses the problems with current rap: a lack of respect for the forefathers of the genre, as well as a lack of focus on any type of lyricism. But, he's also kind enough to ask how it's going.
To help improve the meaning of these lyrics, visit "Turnip the Beet" by Asher Roth Lyrics and leave a comment on the lyrics box


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Seamazz 16/20 Chemical Free Tail-on Raw Shrimp 3 - 2 lb bag
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Our Seamazz all natural raw shrimp is sweet in flavor with a nice, firm texture. Each shrimp is individually quick frozen and comes in a resealable bag, allowing you to thaw just the right quantity for each meal. With no carbohydrates, saturated fat or trans fat, Seamazz raw shrimp is a nutritious source of protein with fewer calories than chicken, turkey, or beef. It can be broiled, grilled, stir fried, sautéed, or steamed and ready to eat in minutes.
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Costco.com products can be returned to any of our more than 600 Costco warehouses worldwide.
Merchandise: We guarantee your satisfaction on every product we sell with a full refund. The following must be returned within 90 days of purchase for a refund: televisions, projectors, computers, cameras, camcorders, touch screen tablets, MP3 players and cellular phones.
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text:
Categories > Celebrities > My Chemical Romance > Stop. Rewind. Replay
Chapter Forty Three
2 ReviewsWill and Gabriel...
Stop. Rewind. Replay
(#) sayDIE 2008-04-16 01:59:37 PM
hey please update soon, I totalllllyyyy looooove this story... :)
you are an awesome writer. :)
I read this today and I didn't stop until I finished it.
well please update soon! :)Author's response
Thank you.
Wow. That's a lotta reading.
I'll try. -
Stop. Rewind. Replay
(#) Alyss 2008-04-21 11:36:56 AM
aawww thats cute, gabriel...aawwww (you can imagine the squealing that has ensued)
aannnd mikeys written a duet...? (that is also vvv cute)
its seems so long since i read this, do you remember all back at chapter one.
it seems so long ago!!
p.s. love u tooAuthor's response
(Oh yes) Thanks!
i have difficulty remembering the last chapter, never mind the first!


text:
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Introducing 24 startups whose founders are building businesses and attempting to disrupt their industries in everything from popsicles to satellites.
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The New York Police Department's stop-and-frisk practices, ruled unconstitutional by a federal judge who was subsequently removed from the case, have led to convictions just 3 percent of the time, the state attorney general's office found.
New York Attorney General Eric T. Schneiderman released a report Thursday showing that 75,000 guilty pleas or conviction at trial resulted from about 2.4 million stops from 2009 to 2012. The tactic has been assailed by civil rights groups, who say it unfairly targets racial minorities.
"It's our hope that this report - the first of its kind - will advance the discussion about how to fight crime without overburdening our institutions or violating equal justice under the law," Schneiderman said in a statement. The attorney general hasn't stated a position on the tactic.
The report used police and court data to examine offenses charged at the time of arrests, whether charges were reduced by the time of convictions, and racial disparities, according to Schneiderman's office.
Only 0.3 percent of all stops, or about 7,200, led to defendants' serving jail or prison sentences for more than 30 days, according to the report. Just 0.1 percent of stops, or about 2,400, led to convictions for violent crimes, it said.
A police spokesman said the report ignores crimes prevented by the practice.
"The report is clearly flawed, which is why it makes absolutely zero recommendations," John J. McCarthy, a spokesman for the department, said in an emailed statement. "This analysis somehow just ignores situations where an officer's action deters or prevents a crime from occurring in the first place. Those situations never result in an arrest, conviction or jail time because a crime is prevented."
A three-judge panel of the U.S. Court of Appeals in Manhattan ruled Wednesday that U.S. District Judge Shira Scheindlin can't challenge her removal from the suit over the stop-and-frisk tactic, which she found unconstitutional.
The court said Scheindlin ran "afoul of the Code of Conduct for United States judges" by making remarks to the press while the case was pending and suggesting that lawyers opposing the tactic use a court rule to steer a case to her. The appeals court said it made no findings of misconduct or bias on her part.
New York City appealed her ruling and won a stay from the appeals court delaying changes she ordered during the appeal.
Mayor Michael Bloomberg criticized Scheindlin's ruling and backed the city's appeal. Bloomberg is the founder and majority owner of Bloomberg LP, the parent of Bloomberg News.
Bill de Blasio, the Democrat elected Nov. 5 to replace Bloomberg as mayor, opposed stop-and-frisk in his campaign and said he will withdraw the city's appeal.
In a separate report released Thursday, the New York Civil Liberties Union said it found a rise in discriminatory policing during the Bloomberg administration.
A survey of 5,000 city residents showed people who had negative contacts with police, such as being stopped and frisked, were less likely than others to turn to the police when in need of help or when witnessing a crime.
"People of color have borne an outsize burden of this injustice, but it impacts every New Yorker and the safety of the city as a whole when entire communities are afraid of the very police force that is supposed to protect them," Donna Lieberman, executive director of the organization, said in a statement.
Community News Network
Stop-and-frisk led to few convictions, state study finds
- Community News Network
Missing Malaysian jetliner confuses a world that's online 24/7
The disappearance five days ago of a Malaysian Airline Systems Bhd. aircraft with 239 people on board is confounding search teams and a global audience used to around-the-clock connectivity and real-time updates.
Which foods are the worst for the environment?
As with most arguments about our food supply, though, it's not that simple. Although beef is always climatically costly, pork or chicken can be a better choice than broccoli, calorie for calorie.
Old Spice attracting women in gender-bending win for P&G
Young women are embracing Old Spice - long known as the brand dad kept in the medicine cabinet - even as P&G's marketing continues to focus on their male peers.
VIDEO: Aftermath of NYC building collapse
Footage from the New York Daily News shows firefighters responding to a building collapse at East 116th Street and Park Avenue in Harlem. At least 11 minor injuries had been reported late Wednesday morning.
VIDEO: Double leads to runaway stroller at spring training game
A fan at a recent Marlins-Mets spring training game in Port St. Lucie was so intent on snagging the ball from a ground rule double by Chris Young that he forgot to hang on to the stroller carrying his young son, which then careened down a hill.
If you were a crustacean, would you feel pain?
A scientist and a seafood chef walk into a bar. "We have a mutual interest," says the scientist. "I study crustaceans and you cook them." But the chef wanted to know just one thing: Do the animals feel pain?
How virus sleuths and public health officials track the cause of a mysterious illness
When a mysterious disease fells people - as happened in California recently, with as many as 20 children experiencing unexplained paralysis - teams of physicians and epidemiologists quickly mobilize. Perhaps you saw the movie "Contagion"? The idea is to find the culprit before it spreads but also to prevent public panic.
First Apple, now Google hit with kids' app lawsuit
Last month, 4- and 5-year-old brothers in New York quickly spent $65.95 in real money to buy virtual goods in Marvel's Run Jump Splash game on the family tablet. They were able to rack up the charges without entering a password. And for that, the boys' mother has joined a class-action lawsuit filed Tuesday against Google, accusing the company of deceiving consumers about its in-app purchase system, which critics say makes it too easy for kids to spend money on their Android devices.
VIDEO: First month of Colorado pot legalization brought in $2M in tax revenue
The first month of recreational marijuana sales in Colorado brought in about $2 million in tax revenue, the first indicator of the earning potential of the U.S.'s premier legal pot market.
VIDEO: NHL game postponed after player collapses
Monday's game between the Columbus Blue Jackets and the Dallas Stars was postponed after Stars forward Rich Peverley collapsed on the bench shortly after completing a shift in the first period.
- More Community News Network Headlines
Missing Malaysian jetliner confuses a world that's online 24/7


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Newegg.com - A great place to buy computers, computer parts, electronics, software, accessories, and DVDs online. With great prices, fast shipping, and top-rated customer service - once you know, you Newegg.
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NVIDIA GeForce GTX 580 is the top of line GPU. With screaming performance and an innovative new thermal design, GeForce GTX 580 rips through the latest games while running quieter than the previous generation. GeForce GTX 580 – don't play DX11 without it.
EVGA announced their lineup of GeForce GTX 580 graphics cards consisting of three versions: NVIDIA reference clock speed version, Superclocked variant and the single-slot FTW Hydro Copper 2 edition. The EVGA GeForce GTX 580 015-P3-1580-RX (reference) is based on the 40 nm GF110 core with 512 CUDA cores, and 1536 MB of GDDR5 memory over a 384-bit memory interface, and supports all the premium GTX gaming technologies such as NVIDIA Surround, 3D Vision, PhysX and SLI.
Filter Results |
Pros: Prompt shipping. Kudos to Newegg! Cons: DOA. Other Thoughts: Second ever RMA through Newegg in 10+ years. Manufacturer Response: Did you find this review helpful? |
AwesomePros: Very fast card with no issues. Love it. Cons: None Other Thoughts: This replaced my ATI 5870. Definitely a faster card. I prefer Nvidia's software over ATI personally. but that is user's choice.. Did you find this review helpful? |
It works! Folds well too...Pros: -Makes for a good SLI partner with discontinued GTX 580s Cons: -Runs 3C warmer than my other GTX 580 under similar loads Other Thoughts: Look, its a refurb! If you need a 580, this is probably worth the gamble. If you are designing a new system, look at the 600-series instead. Did you find this review helpful? |
1out of 3 DOAPros: Super fast card, CUDA performance better than 680 for many applications. Plenty of reviews of the card out there so no need to type too much. :) Cons: Bought two, one was DOA... RMA cost me a week of time, and return shipping... 3rd one worked fine... I wish NewEgg would pay return shipping for faulty merchandise... Other Thoughts: 2 of these in SLI ROCK! for the price hard to beat, if you're willing to gamble on a refurb. Manufacturer Response: 2 out of 2 people found this review helpful. Did you? |
fastest card i have ever ownedPros: came from a gtx 260 asus card to this.... everything can be maxed out now. On A e8500 dual core. Don't walk... run to buy this ! Cons: IS BEING TO FAST A CON ? Other Thoughts: Decided to buy a i5 2500k to remove the bottle neck i am getting with this and my dual core. This thing is that fast. Did you find this review helpful? |
As Good as New!Pros: -Runs flawless* No difference from a brand new model* Cons: None at all. Other Thoughts: This is very clearly stated as refurbished. The card I received has very minimal physical wear or damage on the back side of the board. It did not at all affect the performance of this card. Not an issue as far as I am concerned. 12 out of 12 people found this review helpful. Did you? |


text: Dayton calls for mediator, rejects budget hearing By: Jake Grovum June 2, 2011 0 "I don't understand why this political stunt is being used at is stage of the process," Dayton said at a Capitol news conference. "I've been willing to compromise for weeks now." Subscribe Login Email Address: Password: Already a paid subscriber but not registered for online access yet? For instructions on how to get premium web access, click here.Forgot your password? Share this:EmailPrintFacebookLinkedInTwitterGoogle +1 Amy Koch budget Kurt Zellers Mark Dayton 11:55 am Thu, June 2, 2011 Politics in Minnesota Jake Grovum


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BenFox - 12:06 pm on Dec 30, 2010 (gmt 0)
I've got to train up some linkbuilders in the new year and was wondering if anyone had suggestions of good (free) resources for information on linkbuilding.
When they start they're going to know nothing about SEO but I'm going to have them read the Google SEO Starter Guide and a well known Beginner's Guide.
After that I want to move on to improving their theoretical understanding of the linkgraph before moving on to specific tactics for link building. Finding articles on tactics is easy but I was hoping someone might be able to recommend a good high level resource.
Also any other training material for link builders would be much appreciated.


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St. Patrick's Day — and fleas — in Dublin in the '60s
Guest Post
Note from Dan: Below are parts of two emails I received and cobbled together Sunday by a reader in Roanoke who was responding to Sunday's column. He requested I not use his name.
By Anonymous
Thanks for your piece on Roanoke's St. Patty's Day.
It was my great fortune to spend some time in Dublin, Ireland in the 1960s.
Among the many shocks was to find that the only day of the year the pubs were closed was St.Patrick's Day — this seems to have remained the case for ten or so more years.
Another contrasting shocker was that Easter was more like our July Fourth, Soldiers and tanks were on parade. The Easter Rebellion of 1916 was, of course, the origin.
And St. Patrick's Cathedral was a Protestant place of worship — and had but handful of souls on Sunday. The "Pro-Cathedral," the Catholic venue, had, as you might suppose, people hanging out the windows.
As I recall, Roman Catholics could only attend Trinity College Dublin — home to the "Book of Kells" with the permission of their priest (it was Protestant).
The entire place was a window into 1905 — dirty. poor, foul air from open sod and coal fires and everything black with soot. Nearly every older adult had some type of lung problem.
I would not have been shocked to see Michael Collins, tommy – gun in hand, standing on a running board of a big car turning a corner in the night.
My wife and I returned a few years ago and it is now a different, modern place. The people and Guinness are still grand.
I was there as a 24-year-old 3rd-year med student — and I delivered babies at the Rotunda Hospital (also Protestant) – and in the Dublin slums for three months.
I did not keep track [of the number of babies], and we were generally a small group (med student, nurse mid-wife student, and nurse mid-wife). We often went out on "abnormals" in a taxi cab. "Abnormals" were having some type of problem, from simple to complex.
If it were normal, in-home labor, it meant a fairly long wait — which often meant fleas were on the hunt. We tried to cover our chairs with cleanish sheets, and that helped a bit. An ex-policeman — a nice guy who was fired for IRA connections — was a "porter" in the hospital "lodge" (where we came and went, and they would get us up — or out of the pub etc.), told me how to rid myself of them:
You stand on your bed sheet and strip and shake your clothing. When you see one (human fleas are larger than animal types) you take a bar of soap to it — and after you get them all, you scrape them down your room sink. Worked every time.
It was all amazing.


text:
use the following search parameters to narrow your results:
e.g. subreddit:aww site:imgur.com dog
subreddit:aww site:imgur.com dog
see the search faq for details.
advanced search: by author, subreddit...
~4 users here now
The Linux Mint subreddit: for news, discussion and support for the most popular Linux distribution.
Mouse wobbles- Mint or hardware? (self.linuxmint)
submitted 1 year ago by [deleted]
Hey guys, I'm having issues with my mouse. I have a touch pad, and when I drag left and right across the screen it doesn't move smoothly but wobbles in one spot than jumps to the next. Think electrons in certain orbits. This happened suddenly and right in the middle of working, and the only thing I can think of is that I ran an apt upgrade this morning. Does anyone know how I might fix this or if its a hardware issue?
Edit: that's odd. it went away as I was typing this. I've restarted twice to no avail. Has anyone had these issues? I had a huge update this morning.
[–]dfinf2 0 points1 point2 points 1 year ago
What computer? I had a Thinkpad do this all the time, though not sure if it was particular to Mint as thats all it ever had on there.
[–][deleted] 0 points1 point2 points 1 year ago*
i get that sometimes, but more often I get phantom right-clicks while scrolling. like, i'm scrolling, and all of a sudden the mouse does a right-click and brings up the menu. it's annoying and never happened on ubuntu, but I don't really mind much.
edit: i should add, I get this in MATE on both my dell vostro 1015 and my dell latitude d430. it seems to happen particularly when I try to edge-scroll quickly.
all it takes is a username and password
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create account
is it really that easy? only one way to find out...
already have an account and just want to login?
π Rendered by PID 16782 on app-128 at 2014-03-11 21:58:15.788245+00:00 running 01967df.


text:
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1100 V-Star ClassicThis accessory fits:
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EMEA Shiner's football team by EMEA - over 7 years ago
"Inter-office flag football team. We're an eye care practice, hence the name "shiner""
fast & free shipping, guaranteed delivery dates and all-inclusive pricing
Check out the photos below to see our sports team customers in action. they are wearing custom team t-shirts, custom hoodies, and raglans in all kinds of weather to display their team spirit! CustomInk makes it fun and easy for any team - soccer, softball, or otherwise – to design and order custom team jerseys online.
If you're looking to raise money for your team, be sure to try a team fundraiser selling t-shirts you design. It's easy to set up and absolutely free to launch with no risk to you. Raise $100s for your team easily.
"Inter-office flag football team. We're an eye care practice, hence the name "shiner""
"Halloween 2006 - the back of our Average Joe's t-shirts with personalized nick names"
"Halloween 2006 - Average Joe's Dodgeball team from the movie dodgeball with personalized nick names on the back"
"We hasted a Silent Auction at our office for Ronald McDonald House and this is our team!"
"This photo was taken at a flag football tournament in Maryland. A south asian tournament which benefited the south asian earthquake relief fund. It was great working with CustomInk they provided us with excellent service and fast delivery."
"Sam Brannan Middle School in Sacramento, California has a Robotics team. They build Lego Mindstorm robots and compete with other middle school students all over California. The picture was taken at a tournament in Modesto, California, the first time the team wore their new sweatshirts. Some kids hardly want to take them off! This was the first time the team had professionally made sweatshirts. Previously, we had parents iron on applique. What a difference it made! They really made us look like a team vs "nerds". Customink did a great job. Not only does the sweatshirts have team graphic and team name on front, but each team member has their names and the compeition logo on the back. They even put the graphic low enough so we can read the names below the hoods! We love customink. Everyone was so helpful and patient with us, especially Jenny! Sam Brannan will be hosting a State qualifying tournament December 2, 2006 where the Robowolves will be proudly wearing their customnk sweatshirts. For details, see our web site!"
"This was the last game of the season. The cheerleaders all wore these furry hats to show their spirit."
"We got our shirts last year for our ultimate frisbee team at McGill university in Montreal. This was our championship season. The picture was taken right after we won in early November."
"CustomInk was reliable, cheap and had a speedy deliver, which makes it the only place I would order tshirts online! Our intramural softball team at Loyola College looks better than any other because of our matching shirts, complete with numbers on the back. Thanks CustomInk!"
"We met this AHL hockey player last season while he was sitting out do to an injury. We became good friends, and now have our own section of the rink devoted to him. Bryce Lampman is currently the Assistant Captain for the Hartford Wolfpact."


text:
LiquidPics is a multitouch interactive photo effecter. What you see is what you beleave, check following video.
Archive for October, 2008
Wednesday, October 22nd, 2008Old Camera
Wednesday, October 22nd, 2008Old Camera is limited version of Toy Camera. Whenever you take a photo with this app, you can get old black and white picture.
Now we have a flickr community!
Toy Camera
Wednesday, October 22nd, 2008Toy Camera simply converts iPhone's cheap camera to lovely toy camera like lomo.
Now we have a flickr group!
This app has no setting panel, no complex feature, just take a photo and you get nicely effected pictures.
Random Pose Viewer
Wednesday, October 22nd, 2008Random Pose Viewer is a free drawing study tool which is provided by posemaniacs.com
This app randomly displays more than 19000 poses as drawing motif.
Basic functionality is same as an online flash version except, this has a zooming feature with pinch gesture.


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Rank Boston's biggest sports busts
The list of players who never lived up to their potential or their contracts while in Boston seems to grow each year. Boston.com's Steve Silva compiled a review of some of the biggest busts in Boston sports history, and now we're giving you the chance to rank your five biggest busts and compare them to Silva's and other readers.
Discuss: Let us know who else should be on the list | View results
Your biggest busts
Thanks for your contribution!
Shows leading vote-getters from all ballots submitted
Steve Silva
- 1 Carl Crawford
- 2 Rick Pitino
- 3 John Lackey
- 4 Vin Baker
- 5 Daisuke Matsuzaka


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Kai The Hitchhiker Wanted For Murder! Authorities have issued an arrest warrant for internet celebrity "Kai" in the murder of Joseph Galfy, Jr. in Clark, New Jersey. The suspect is identified as Caleb Lawrence McGillvary, a 24-year-old Internet celebrity and is well known on the inernet as"Kai the Hatchet Wielding Hitchhiker." 5/16/2013 5:06:58 PM 14,928
Kai The Hatchet Wielding Hitchhiker An epic interview with Kai, a hitchhiker who used his hatchet to stop a man that thought he was Jesus from killing people in Fresno, CA. 5/16/2013 5:05:23 PM 14,738
CNN Busted For Faking Satellite Interview Major fail by CNN. What was the point of making them seem like they were far apart? 5/9/2013 11:42:59 AM 35,188
Black Man Describes Captive White Girls Rescue Once again, black people give the best dramatic interviews! 5/6/2013 10:59:46 PM 20,502
Complete Failure at News Station Talk about fail sauce...nothing goes right over there. 5/5/2013 8:45:06 AM 26,890
First Time News Anchor Fail His career as a news anchor is over before it even started. 4/22/2013 12:15:52 PM 47,397
Woman's Epic Description of Strong Hail Storm Black women always give the best interviews! 3/21/2013 2:21:25 PM 49,293
What Happens When You Inhale Sulfur Hexafluoride Sulfur Hexafluoride is a gas that is 6 times heavier then what we breath. It has some very cool effects. 3/5/2013 11:26:52 AM 20,381


text:
shortcuts Rated 4 out of 5 stars
I would like to enter a shortcut to insert the disposable mail (like ctrl+alt+c).
and one other thing, since I have the option to open the mail inbox once the disposable mail on, most website demand to enter the mail twice (as confirmation), so when entering the disposable mail in both boxes, I get two new tabs of the inbox opened.
I don't know how you can find a workaround for it.
Perfect Addon Rated 4 out of 5 stars
it makes my life easier.
but the only thing I can't seem to figure it out it the keyboard shortcut ! i tried pressing ctrl+y alt+y but didn't work.
No shortcut available
Hi. I'm really sorry, but as far as I remember, I haven't set any keyboard short-cut in this extension. Can you give more details about what you would like to do ?


text:
What is STEM?
STEM@SU Factsheet
STEM Scholarship Opportunities @SU
STEM Majors
STEM Education Majors
STEM Graduate Programs
STEM Summer Research Opportunities @SU (Updated 1/31/14)
STEM Outreach/Activities: Science Nights, Science Olympiad, Science Camp, Center for Applied Mathematics and Science (CAMS), STEM Living-Learning Communities (Updated 3/07/14)
Eastern Shore Regional Tournament of the Maryland Science Olympiad (Updated 3/07/14)
Scholarships/Fellowships/Internships for STEM Students (Updated 1/27/14)
What's New in STEM@SU? (Updated 2/20/14)
NSF-funded Bridges for SUCCESS Program
STEM Career Information
STEM Listserv
Eastern Shore STEM Network
Biology and Chemistry Seminars
News Archive
Give Now
Find us on Facebook
Follow us on Twitter @SU_STEM
Kristen A. Paul STEM Coordinator 410-677-5368 email@example.com
STEM E-mail: firstname.lastname@example.com
FBI Information Session
A Special Agent with the FBI will be on campus to talk about opportunities available to college students
Tuesday, November 29, 2011
from 2:30 PM - 3:25 PM
To attend this event, please sign up online through e-Recruiting:
Go to http://salisbury.experience.com/er/security/login.jsp and log in (login instructions to the left of the page)
Once you are in - you may have to enter some general profile data first if you have never been in eRecruiting - click on "Calendar" on the grey menu bar and you will see a drop-down. Select "Event Type"
From the "Event Type" drop-down, select "Employer Information Session"
Click on the workshop you wish to attend
On the right of the page you will see a link that says "Sign-up". Click on it.
If you have any problems registering, contact
Mr. Endicott in Career Services at
email@example.com or call 410-543-6075


text:
Excerpt: "We seek to examine how current policies and Administration efforts affect opportunities for enhancing or expanding U.S. manufacturing and competitiveness, both for strengthening domestic job growth and for the benefit of U.S. influence over international nuclear safety and nuclear security."
Chairman: Ed Whitfield (KY)
Vice Chairman: Steve Scalise (LA)
Ralph Hall (TX)
John Shimkus (IL)
Joseph R. Pitts (PA)
Lee Terry (NE)
Michael C. Burgess, M.D. (TX)
Bob Latta (OH)
Bill Cassidy (LA)
Pete Olson (TX)
David McKinley (WV)
Cory Gardner (CO)
Mike Pompeo (KS)
Adam Kinzinger (IL)
Morgan Griffith (VA)
Joe Barton (TX)
Fred Upton (MI)
Ranking Member: Bobby L. Rush (IL)
Jerry McNerney (CA)
Paul Tonko (NY)
John Yarmuth (KY)
Eliot L. Engel (NY)
Gene Green (TX)
Lois Capps (CA)
Michael F. Doyle (PA)
John Barrow (GA)
Doris O. Matsui (CA)
Donna M. Christensen (VI)
Kathy Castor (FL)
John D. Dingell (MI) (non-voting)
Henry A. Waxman (CA)
WASHINGTON, DC – One year ago today, President Obama stood in front of a pile of steel pipes in Cushing, Oklahoma, and endorsed the southern leg of the Keystone XL pipeline, calling for action to cut red tape and mak
WASHINGTON, DC – The Subcommittee on Energy and Power, chaired by Rep. Ed Whitfield (R-KY), today continued its American Energy Security and Innovation hearing series with a focus on natural gas and electric coordination challenges.
U.S. Rep. Adam Kinzinger: We need the Keystone XL pipeline
By: U.S. Representative Adam Kinzinger (IL-16)
To read the cover letter to Energy and Commerce Committee Ranking Member Henry Waxman, click here.


text:
Market News
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Better-than-expected domestic data and encouraging international political developments propelled benchmarks into positive territory. Meanwhile, investor enthusiasm for a couple of sectors catapulted the S&P 500 and the Nasdaq to new highs. All the S&P 500 industry groups finished in the green, among which, materials stocks emerged as the biggest gainer.
The Dow Jones Industrial Average (DJI) increased 0.7% to close the day at 14,818.75. The S&P 500 gained 0.7% to finish yesterday's trading session at 1,593.61. The tech-laden Nasdaq Composite Index rose 0.9% to end at 3,307.02. The fear-gauge CBOE Volatility Index (VIX) gained 0.7% to settle at 13.71. Consolidated volumes on the New York Stock Exchange, American Stock Exchange and Nasdaq were roughly 5.1 billion shares, well below 2012's average of 6.48 billion shares. Advancing stocks outnumbered the decliners. For the 73% that advanced, 24% declined.
The S&P 500 closed at an all-time high while the tech-laden Nasdaq rose to its highest level in more than a decade after investors turned bullish towards the energy and technology sectors. Continuation of monetary support, in light of low inflation from the Federal Reserve and expectations of rate cuts from the European Central Bank (ECB) added to investor confidence.
Meanwhile, better-than-expected housing data also lifted investor sentiment. According to the National Association of Realtors, pending home sales improved in March. In percentage terms, pending home sale increased 1.5% to 105.7 from 104.1 in February and is 7% higher on a year-over-year basis. This was higher than the consensus estimate of 0.7%. According to the U.S. Department of Commerce, personal income and disposable personal income (DPI) each increased 0.2% in comparison to an increase of 1.1% each in personal income and DPI, in February. The PCE inflation for March decreased 0.1% versus an increase of 0.4% in the previous quarter.
Investors also received a boost from the formation of a new government in Italy. After two months of a political gridlock, Italy finally formed a government and Enrico Letta was sworn in as Italian Prime Minister on Sunday. Letta laid emphasis on reviving growth and increasing employment in Italy. He added that he plans to ease taxes on young and new employees; cancel planned raise in the value-added tax and restructure the property tax. He also said bringing about tax reforms without increasing debt would be difficult.
On the earnings front, shares of Roper Industries, Inc. (NYSE:ROP) lost 3.8% after its revenue missed the Street's estimates. Shares of Health Net, Inc. (NYSE:HNT) increased 4.6% after its earnings soared past the Street's expectations. Adjusted earnings per share (EPS) were recorded at $0.62 in comparison to the Street's expectation of $0.41. The company's revenue of $2.8 billion marginally beat estimates of $2.79 billion.
According to Thomson Reuters, about 274 S&P 500 companies have released earnings of which 69% have beaten the Street's expectations and 43.2% have registered revenues above estimates.
Of the top ten S&P 500 industry groups, materials stocks were the biggest gainers. The Materials Select Sector SPDR (XLB) gained 1.5%. Stocks such as Monsanto Company (NYSE:MON), the Dow Chemical Company (NYSE:DOW), E I Du Pont De Nemours And Co (NYSE:DD), FMC Corp (NYSE:FMC) and Praxair, Inc. (NYSE:PX) increased 1.7%, 1.4%, 2.3%, 1.3% and 1.5%, respectively.
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InspectAPedia ® Home HEATING SYSTEMS AIR CONDITIONING & HEAT PUMP SYSTEMS BACKDRAFTING HEATING EQUIPMENT BACKUP HEAT for HEAT PUMPS BANGING HEATING PIPES RADIATORS BAROMETRIC DAMPERS BASEBOARD HEAT BLOWER FAN OPERATION & TESTING BOILERS, HEATING BOOKSTORE - InspectAPedia BTU USAGE MONITORS CARBON MONOXIDE - CO CIRCULATOR PUMPS & RELAYS COMBUSTION AIR CONDENSING BOILERS/FURNACES DIAGNOSTIC GUIDES A/C / HEAT PUMP DIAGNOSE & FIX HEATING PROBLEMS-BOILER DIAGNOSE & FIX HEATING PROBLEMS-FURNACE DIRECT VENTS / SIDE WALL VENTS DRAFT REGULATORS, DAMPERS, BOOSTERS DUCT SYSTEM & DUCT DEFECTS ELECTRIC HEAT, DIAGNOSIS, REPAIR ELECTRIC MOTOR DIAGNOSTIC GUIDE FAN, AIR HANDLER BLOWER UNIT FLOODED HEATING EQUIPMENT REPAIR FLUE SIZE SPECIFICATIONS FURNACES, HEATING GAS BURNER Flame & Noise Defects GAS PIPING, VALVES, CONTROLS GEOTHERMAL HEATING SYSTEMS HEAT PUMPS, DIAGNOSIS, REPAIR HEATING COST SAVINGS METHODS HEATING OIL PIPING TROUBLES HEATING OIL TANKS HEATING SYSTEM NOISES HEATING SYSTEM TYPES GAS LP & NATURAL GAS SAFETY HAZARDS MANUALS & PARTS GUIDES - HVAC MIXING / ANTI-SCALD VALVES MOTOR OVERLOAD RESET SWITCH NOISE, HEATING SYSTEMS ODORS FROM HEATING SYSTEMS OIL BURNERS OIL FILTERS on HEATING EQUIPMENT OIL FILL PIPE LEAKS OIL SPILL CLEANUP / PREVENTION OIL TANKS PLASTIC Plexvent / Ultravent RECALL PUFFBACKS, OIL BURNER RADIANT HEAT RADIATORS RELIEF VALVE LEAKS Reset Switch - Heater Primary Control RESET SWITCH - ELECTRIC MOTOR Reset Switch - Stack Relays SAFETY, HEATING INSPECTION SAFETY RECALLS CHIMNEYS VENTS HEATERS SOLAR HEATING SYSTEM DESIGNS SOOT on OIL FIRED HEATING EQUIPMENT STEAM HEATING SYSTEMS TANKLESS COILS THERMOSTATS, HEATING / COOLING VIDEO GUIDES: Heating System Videos WATER HEATERS WINTERIZE A BUILDING WOOD, COAL STOVES & FIREPLACES WOOD STOVE SAFETY ZONE DAMPERS ZONE VALVES, HEATING More Information |
This article gives a definition of AFUE and explains AFUE (Annual Fuel Utilization Efficiency) or heating system efficiency ratings and numbers. The articles at this website describe the basic components of a home heating system, how to find the rated heating capacity of an heating system by examining various data tags and components, how to recognize common heating system operating or safety defects, and how to save money on home heating costs. Green links show where you are. © Copyright 2014 InspectApedia.com, All Rights Reserved. How is heating boiler efficiency or economy measured? What does boiler AFUE mean?How is heating boiler efficiency or economy measured? What does boiler AFUE mean?In short, the AFUE (Annual Fuel Utilization Efficiency) tells you, Here's a very rough example: if the AFUE number for your heater is 80%, that means that for every dollar you spend on heat, twenty cents "goes up the chimney" or is lost in various other ways, and eighty cents of each heating dollar shows up in your occupied space as heat. Each model of heating boiler or furnace is assigned an AFUE number. Our page top sketch of a high efficiency furnace is provided courtesy of Carson Dunlop Associates. At left we illustrate a high efficiency gas furnace - expected to have a high AFUE number. Sketch courtesy of Carson Dunlop Associates. Also see this sketch of a high efficiency condensing furnace and this high efficiency pulse combustion heating system. AFUE is an abbreviation for Annual Fuel Utilization Efficiency. In short, the AFUE tells you, for each dollar you spend on energy for heating by gas, oil, or another fuel, just how much of your dollar shows up inside the occupied space of your building as heat. Higher AFUE is better. If your boiler or furnace or steam boiler has an AFUE rating of 90, that means that for every dollar you spend on fuel, 90 cents worth of heat is delivered into your building. The remaining 10 cents is lost in inefficiency such as heat that escapes up the chimney along with the products of combustion. What is the Reliability & Accuracy of Heating System Efficiency Ratings - AFUE?AFUE is not the whole story of heating cost efficiency. A high-efficiency heating system that has not been cleaned and serviced may be running poorly and wasting money. In fact, an 85% AFUE heating boiler that has not been cleaned might be running at an efficiency much lower, perhaps 65%. Furthermore, if your building is drafty or poorly insulated, you may be delivering heat at high efficiency but losing it from the building much faster than necessary. These articles can help with a more complete approach to saving money on heat:
What are the Current AFUE Requirements for Heating Equipment?
Other Heating System Performance Measurements & StandardsAlso see our definition of HSPF, SEER, and other measurements found at SEER RATINGS & OTHER DEFINITIONS More ReadingGreen link shows where you are in this article series.
Frequently Asked Questions (FAQs) about the definition of AFUE or heating system efficiency measurements... Ask a Question or Search InspectApediaQuestions & answers or comments about AFUE and heating system efficiency ratings, measurement, and improvement Try the search box just below or if you prefer, post a question or a comment in the Comments box below and we will respond promptly. Search the InspectApedia website HTML Comment Box is loading comments...
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- Two Mammalian MOF Complexes Regulate Transcription Activation by Distinct Mechanisms
Two Mammalian MOF Complexes Regulate Transcription Activation by Distinct Mechanisms
Molecular Cell, Volume 36, Issue 2, 23 October 2009, Pages 290-301
Xiangzhi Li, Lipeng Wu, Callie Ann Sprunger Corsa, Steve Kunkel and Yali Dou
SummaryIn mammals, MYST family histone acetyltransferase MOF plays important roles in transcription activation by acetylating histone H4 on K16, a prevalent mark associated with chromatin decondensation, and transcription factor p53 on K120, which is important for activation of proapoptotic genes. However, little is known about MOF regulation in higher eukaryotes. Here, we report that the acetyltransferase activity of MOF is tightly regulated in two different but evolutionarily conserved complexes, MSL and MOF-MSL1v1. Importantly, we demonstrate that while the two MOF complexes have indistinguishable activity on histone H4 K16, they differ dramatically in acetylating nonhistone substrate p53. We further demonstrate that MOF-MSL1v1 is specifically required for optimal transcription activation of p53 target genes both in vitro and in vivo. Our results support a model that these two MOF complexes regulate distinct stages of transcription activation in cooperation with other histone modifying activities.
Summary | Full Text | PDF (1160 kb) - Physical Association and Coordinate Function of the H3 K4 Methyltransferase MLL1 and the H4 K16 Acet...
Physical Association and Coordinate Function of the H3 K4 Methyltransferase MLL1 and the H4 K16 Acetyltransferase MOF
Cell, Volume 121, Issue 6, 17 June 2005, Pages 873-885
Yali Dou, Thomas A. Milne, Alan J. Tackett, Edwin R. Smith, Aya Fukuda, Joanna Wysocka, C. David Allis, Brian T. Chait, Jay L. Hess and Robert G. Roeder
SummaryA stable complex containing MLL1 and MOF has been immunoaffinity purified from a human cell line that stably expresses an epitope-tagged WDR5 subunit. Stable interactions between MLL1 and MOF were confirmed by reciprocal immunoprecipitation, cosedimentation, and cotransfection analyses, and interaction sites were mapped to MLL1 C-terminal and MOF zinc finger domains. The purified complex has a robust MLL1-mediated histone methyltransferase activity that can effect mono-, di-, and trimethylation of H3 K4 and a MOF-mediated histone acetyltransferase activity that is specific for H4 K16. Importantly, both activities are required for optimal transcription activation on a chromatin template in vitro and on an endogenous MLL1 target gene, Hox a9, in vivo. These results indicate an activator-based mechanism for joint MLL1 and MOF recruitment and targeted methylation and acetylation and provide a molecular explanation for the closely correlated distribution of H3 K4 methylation and H4 K16 acetylation on active genes.
Summary | Full Text | PDF (720 kb) - MOF-Regulated Acetylation of MSL-3 in the Drosophila Dosage Compensation Complex
MOF-Regulated Acetylation of MSL-3 in the Drosophila Dosage Compensation Complex
Molecular Cell, Volume 11, Issue 5, 1 May 2003, Pages 1265-1277
Alessia Buscaino, Thomas Köcher, Jop H Kind, Herbert Holz, Mikko Taipale, Kerstin Wagner, Matthias Wilm and Asifa Akhtar
SummaryDosage compensation ensures equal expression of X-linked genes in males and females. In Drosophila, equalization is achieved by hypertranscription of the male X chromosome. This process requires an RNA/protein containing dosage compensation complex (DCC). Here we use RNA interference of individual DCC components to define the order of complex assembly in Schneider cells. We show that interaction of MOF with MSL-3 leads to specific acetylation of MSL-3 at a single lysine residue adjacent to one of its chromodomains. We observe that localization of MSL-3 to the X chromosome is RNA dependent and acetylation sensitive. We find that the acetylation status of MSL-3 determines its interaction with roX2 RNA. Furthermore, we find that RPD3 interacts with MSL-3 and that MSL-3 can be deacetylated by the RPD3 complex. We propose that regulated acetylation of MSL-3 may provide a mechanistic explanation for spreading of the dosage compensation complex along the male X chromosome.
Summary | Full Text | PDF (636 kb) - …more
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Copyright © 2000 Cell Press All rights reserved.
Molecular Cell, Volume 5, Issue 2, 367-375, 1 February 2000
Add/View Comments (0)Activation of Transcription through Histone H4 Acetylation by MOF, an Acetyltransferase Essential for Dosage Compensation in Drosophila
Asifa Akhtar1, 2 and Peter B Becker1, 2, ‡, *,
1 The Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
2 Adolf Butenandt-Institut, Molekularbiologie, Schillerstrasse 44, Ludwig-Maximilians-Universität München, 80336 München, Germany
- Dosage compensation in Drosophila involves a 2-fold increase in transcription from the single male X relative to the two female X chromosomes. Regulation at the level of the chromosome involves alterations in chromatin organization: male X chromosomes appear decondensed and are marked by acetylation of histone H4 at lysine 16. We demonstrate that MOF, a protein required for dosage compensation with significant sequence similarity to the MYST family of acetyltransferases, is a histone acetyltransferase that acetylates chromatin specifically at histone H4 lysine 16. This acetylation relieves chromatin-mediated repression of transcription in vitro and in vivo if MOF is targeted to a promoter by fusion to a DNA-binding domain. Acetylation of chromatin by MOF, therefore, appears to be causally involved in transcriptional activation during dosage compensation.


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Tarsha Jackson killed in Northwest D.C. hit-and-run
(AP/ABC7) - A 16-year-old has died after being hit by a car in Northwest Washington, and police are asking for the public's help in locating a vehicle involved in the crash.
The Metropolitan Police Department said Tarsha Jackson died Saturday at about 2:15 p.m. The girl, who police said was from Northwest Washington, was struck Friday around 6 p.m. at the intersection of First Street and New York Avenue NW.
Police say the accident happened when a 2003 Pontiac Bonneville that had a green light hit the girl as she crossed the road outside of a crosswalk. A tan sport utility vehicle with D.C. registration then hit the girl.
The Pontiac remained on the scene, but the SUV did not stop.
"I just can't put it in my head," says Tiara Jackson, Tarsha's sister. "She's still here to me."
Her sister spotted the flashing lights and made the horrifying discovery.
"I was going to look for her. I saw the street blocked off and when I walked that way," she says. "I could see her stuff lying in the street."
Tarsha's family believes the teen was the target of a crime before the accident.
Relatives say Tarsha was wearing a pink Helly Hanson coat when she was struck. They say a witness told them the teen was running from a male trying to steal her coat.
"The boy had on a big black coat. He was chasing Tarsha across the street...that's what the girl told us," Tarsha's cousin, Ciera Pollard, said.
The Helly Hanson brand has been the focus of robberies in the city.
Police are investigating the witness' account but did not confirm any information about a would-be robbery.
Tarsha was the youngest of six children. Now her family is left doing the unexpected - planning and raising money for a funeral and trying to understand how anyone could run over a child and never look back.
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Description: The Ridgecrest Daily Independent has an immediate opening to take over the Rocketeer II, a publication that serves the Naval Air Weapons Station, China Lake. The editor is expected to work closely with the NAWS China Lake Public Affairs Officer to develop content for this publication that comes out every two weeks. This will include covering stories on the base, taking photos, editing submitted content and laying out pages under a strict deadline. Knowledge of Quark is a must. Previous experience working with the Military is a plus, but not required. An applicant must be able to start immediately. China Lake is a cutting-edge research and testing facility in the Mojave Desert of Eastern Kern County. It is one of the largest land-based facilities in the Navy. It is home to multiple naval commands, all of which work with the Rocketeer II to produce articles and content for each edition. China Lake is a gem in the Navy.The base is nestled in the Eastern Sierra within the Indian Wells Valley. The city of Ridgecrest is the support community, which houses nearly all the base employees and has a strong symbiotic relationship with the base. Ridgecrest is within three hours of Las Vegas and Los Angeles and a short day trip away from Death Valley, Mt. Whitney and Mammoth Lakes. It has plenty of outdoors activates for the adventurous and a strong small-town atmosphere. A successful applicant must be able to hit the ground running and learn quickly. Because the content focuses on the Navy base, all stories and photos are subject to approval from the Navy. We stress being a strong team member and going above and beyond to ensure the production of a quality product. Applicants must show the ability to juggle multiple tasks and be flexible. There is also the opportunity to work on special projects for the Daily Independent. Please send a resume, cover letter, writing and layout samples and a list of references to John Watkins at firstname.lastname@example.com.
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