text:
National Cancer Institute®
Last Modified: August 1, 2002
AU - Verma S; Bramwell V
Dose-intensive chemotherapy in advanced adult soft tissue sarcoma.
SO - Expert Rev Anticancer Ther 2002 Apr;2(2):201-15
AD - University of Ottawa, 503 Smyth Road, Ottawa, ON, Canada.
The treatment of metastatic soft tissue sarcomas in adults is one of the
most challenging areas in oncology. While multidisciplinary management
of early-stage, localized disease has led to a number of improved
outcomes, therapy of unresectable or advanced disease remains
problematic. Virtually every conventional cytotoxic agent has been
systematically assessed in this malignancy, yet only a handful have
demonstrated significant activity. Adriamycin and ifosfamide are the
only chemotherapeutic drugs to have consistently produced response rates
of over 20% when given as single agents and these two drugs have been
exhaustively studied alone or in combination. Recent efforts to improve
response rates and, by inference, disease-free survival and overall
survival, have involved exploration of high-dose regimen incorporating
growth factors and/or autologous cellular support. In this article, the
status of dose-intensive chemotherapy in advanced adult soft tissue
sarcomas (excluding pediatric histologies, such as Ewing's sarcoma and
rhabdomyosarcoma) will be discussed. Emphasis will be placed on data
from randomized Phase III trials but information from Phase I/II studies
will also be reviewed and recommendations will be made on a systematic
analysis of the data.
AU - Soderstrom M; Bohling T; Ekfors T; Nelimarkka L; Aro HT; Vuorio E
Molecular profiling of human chondrosarcomas for matrix production and
cancer markers.
SO - Int J Cancer 2002 Jul 10;100(2):144-51
AD - Skeletal Research Program, Department of Medical Biochemistry and
Molecular Biology, University of Turku, Turku, Finland.
Chondrosarcoma is the second most common malignant bone tumor,
characterized by production of abundant extracellular matrix resembling
hyaline cartilage. To better understand the molecular pathogenesis of
chondrosarcoma, we analyzed 12 chondrosarcomas for their production of
connective tissue components and SOX9, a key regulator of normal
chondrocyte differentiation. Furthermore, 10 chondrosarcoma samples were
screened for additional changes in gene expression using cDNA array
analysis. In Northern analysis, several tumors were found to express
type II collagen mRNA at levels comparable to fetal cartilage used as a
control. Interestingly, the highest levels of type II collagen mRNA were
seen in 2 of the 3 grade 3 chondrosarcomas, which also exhibited the
highest mRNA levels of SOX9 and "prechondrogenic" pro alpha 1(IIA)
collagen. Expression of SOX9 in human chondrosarcomas is novel and
suggests that chondrosarcomas originate from a multipotent stem cell
committed to differentiation along the chondrogenic pathway. Results of
the cDNA array analyses emphasize the heterogeneous nature of
chondrosarcoma as no single transcript was systematically up- or
downregulated in all tumors analyzed. Among the interesting changes
observed was upregulation of decorin mRNA in 7 of the 10 tumors
analyzed. Further studies are needed to determine whether decorin plays
a role in the pathogenesis of chondrosarcoma. The cDNA arrays also
revealed discrepancies from Northern and RNase protection analyses in
transcript levels of matrix components, emphasizing the need to validate
cDNA array data with other techniques. Copyright 2002 Wiley-Liss, Inc.
AU - Wendtner CM; Abdel-Rahman S; Krych M; Baumert J; Lindner LH; Baur A;
Hiddemann W; Issels RD
Response to neoadjuvant chemotherapy combined with regional hyperthermia
predicts long-term survival for adult patients with retroperitoneal and
visceral high-risk soft tissue sarcomas.
SO - J Clin Oncol 2002 Jul 15;20(14):3156-64
AD - Department of Internal Medicine III, Diagnostic Radiology and Institute
for Biostatistics and Epidemiology, Klinikum Grosshadern Medical Center,
Ludwig-Maximilians-University, Munich, Germany.
PURPOSE: To determine the efficacy of neoadjuvant chemotherapy combined
with regional hyperthermia (RHT) for local tumor control and overall
survival (OS) in adult patients with retroperitoneal or visceral (RP/V)
high-risk soft tissue sarcomas (HR-STS). PATIENTS AND METHODS: From 1991
to 1997, 58 patients with HR-STS at RP/V sites were prospectively
treated with four cycles of etoposide, ifosfamide, and doxorubicin
combined with RHT followed by surgery, adjuvant chemotherapy, and
radiation. RESULTS: Objective response rate assessable in 40 patients
was 13% (five partial responses). Including minor responses (n = 8), the
radiographic response rate was 33%. The pathologic response rate
assessable in 26 patients after surgical resection was 42%. Median OS
was 31 months. At a median observation time of 74 months, 5-year
probability of local failure-free survival (LFFS), distant
metastasis-free survival, event-free survival, and OS were 25%, 51%,
20%, and 32%, respectively. Averaged minimum temperatures (T(min)) and
time-averaged temperatures achieved in 50% (T(50)) and 90% (T(90)) of
all measured tumor sites differed significantly between responders and
nonresponders (T(min), 39.3 degrees C v 38.0 degrees C; P =.002; T(50),
40.9 degrees C v 40.3 degrees C; P =.038; T(90), 40.1 degrees C v 39.3
degrees C; P =.017). At 5-year follow-up, probability of LFFS (59% v 0%;
P <.001) and OS (60% v 10%; P <.001) was significantly in favor of
patients responding to neoadjuvant thermochemotherapy. CONCLUSION:
Response to neoadjuvant chemotherapy combined with RHT is predictive for
an improved local tumor control resulting in a long-term survival
benefit for patients with HR-STS at unfavorable RP/V sites; however, the
impact of RHT has to be defined in a randomized phase III trial.
AU - Little DJ; Ballo MT; Zagars GK; Pisters PW; Patel SR; El-Naggar AK;
Garden AS; Benjamin RS
Adult rhabdomyosarcoma: outcome following multimodality treatment.
SO - Cancer 2002 Jul 15;95(2):377-88
AD - Department of Radiation Oncology, University of Texas M.D. Anderson
Cancer Center, Houston, Texas 77030, USA.
BACKGROUND: Childhood rhabdomyosarcoma (RMS) has a relatively good
prognosis. Outcome for adults with this disease is poorly documented due
to its rarity. METHODS: The clinicopathologic features, treatment
methods, and disease outcome were reviewed retrospectively for 82 adults
with locoregional RMS treated between 1960 and 1998. Patients with
distant metastasis at diagnosis were excluded. Actuarial univariate and
multivariate statistical methods were used to evaluate outcome. RESULTS:
Patient ages ranged from 17 to 84 years (median, 27 years). Histologic
subtypes were embryonal (34%), pleomorphic (43%), and alveolar (23%).
Anatomic sites of origin were head and neck (52%), trunk (26%), and
extremity (7%). Tumor size was 5 cm or smaller in 51% of patients.
Regional lymph node metastasis was present in 33% of patients at
presentation. Treatment consisted of radiation alone in 11%, radiation
and surgery in 18%, radiation and chemotherapy in 34%, and all three
modalities in 37%. With a median follow-up of 10.5 years, the 10-year
actuarial disease-free and overall survival rates were 41% and 40%,
respectively. The 10-year actuarial local, lymph node, and metastatic
control rates were 75%, 82%, and 53%, respectively. The major
determinant of metastatic control and survival was primary tumor size (<
or = 5 vs. > 5 cm). Local control was satisfactory (10-year rate of 87%)
for sites other than parameningeal (50% at 10 years). Patients whose
disease responded to chemotherapy had a significantly better metastasis
free period (72% at 10 years) than those whose disease failed to respond
(19% at 10 years). CONCLUSIONS: Adult RMS is a highly malignant tumor
with a significant incidence of metastatic recurrence. Continuing
investigation of new and potentially more effective chemotherapy is
crucial. Local control is satisfactory for sites other than
parameningeal where new radiation technologies such as
intensity-modulated therapy may be necessary to safely deliver adequate
doses. Copyright 2002 American Cancer Society.DOI 10.1002/cncr.10669
AU - Maire G; Pedeutour F; Mrozek K; Rys J; Iliszko M; Limon J
COLIA1-PDGFB gene fusion in dermatofibrosarcoma protuberans. molecular
analysis of a case with an unusual large marker containing sequences
from chromosomes 7, 8, 17, 21, and 22.
SO - Cancer Genet Cytogenet 2002 Jun;135(2):197-9
AU - Fisher B; Won M; Macdonald D; Johnson DW; Roa W
Phase II study of topotecan plus cranial radiation for glioblastoma
multiforme: results of Radiation Therapy Oncology Group 9513.
SO - Int J Radiat Oncol Biol Phys 2002 Jul 15;53(4):980-6
AD - Department of Radiation Oncology, London Regional Cancer Centre, London,
Ontario, Canada. firstname.lastname@example.com
PURPOSE: A Phase II trial was conducted by the Radiation Therapy
Oncology Group (RTOG) to compare the survival of patients with
glioblastoma multiforme treated with topotecan combined with standard
cranial radiotherapy (RT) for matched patients treated in prior RTOG
studies. A secondary objective was to document the acute and late
toxicities of this combination of chemotherapy and RT. METHODS AND
MATERIALS: Eighty-seven patients with histologically confirmed
glioblastoma multiforme received standard cranial RT (60 Gy/30 fractions
in 6 weeks) plus topotecan 1.5 mg/m2 per day i.v. for 5 d/wk every 3
weeks for 3 cycles. Eighty-four patients were evaluated, of whom 60
(71%) were > or =50 years, 44 (52%) were men, and 61 (73%) had a
Karnofsky performance status of > or =80. Twenty-nine percent of
patients had undergone biopsies, 48% partial resections, and 21% gross
total resections. Two resections were unspecified as to the extent of
tumor removal. Fourteen percent of patients were recursive partitioning
analysis Class III, 46% were Class IV, 35% were Class V, and 5% were
Class VI. RESULTS: The median survival was 9.3 months. Sixty-seven
patients (80%) had progression. The 1-year survival rate was 32%. One
patient remained alive without recurrence. RTOG 9513 patients were
matched with patients in an RTOG clinical trial database from previous
clinical trials. The matching variables were age, Karnofsky performance
status, mental status, and prior surgery. No statistically significant
difference was found between the survival of the study patients and that
of the matched patients from the RTOG database. Fifty-four percent of
patients had Grade IV acute toxicity. The toxicity was primarily
hematologic. Four patients had Grade III late central nervous system
toxicities. CONCLUSION: Topotecan administered at a dose of 1.5 mg/m2
per day i.v. for 5 d/wk every 3 weeks for 3 cycles given concurrently
with standard cranial RT for glioblastoma does not produce a
statistically significant survival advantage over previously tested
therapies. Other methods of administration of topotecan or other
camptothecins may provide more effective radiosensitization.
AU - AuCoin DP; Colletti KS; Xu Y; Cei SA; Pari GS
Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) contains
two functional lytic origins of DNA replication.
SO - J Virol 2002 Aug;76(15):7890-6
AD - Department of Microbiology and Cell and Molecular Biology Program,
University of Nevada School of Medicine, Reno, Nevada 89557, USA.
We used a transient-transfection replication assay to identify two
functional copies of the human herpesvirus 8 (HHV8) lytic origin of DNA
replication (oriLyt). BCLB-1 cells were transfected with HHV8 subgenomic
fragments containing the putative lytic origin along with a plasmid
expressing viral transactivator open reading frame (ORF) 50. The HHV8
left-end oriLyt (oriLyt-L) lies between ORFs K4.2 and K5 and is composed
of a region encoding various transcription factor binding sites and an
A+T-rich region and a G+C repeat region. The right-end oriLyt (oriLyt-R)
maps between ORF 69 and vFLIP, a region similar to the RRV oriLyt, and
is an inverted duplication of oriLyt-L.
AU - Thompson LD; Gannon FH
Chondrosarcoma of the larynx: a clinicopathologic study of 111 cases
with a review of the literature.
SO - Am J Surg Pathol 2002 Jul;26(7):836-51
AD - Department of Endocrine and Otorhinolaryngic-Head & Neck Pathology,
Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA.
Chondrosarcomas of the larynx are rare tumors accounting for about 0.5%
of all laryngeal primary tumors. A total of 111 laryngeal chondrosarcoma
cases, diagnosed between 1970 and 1997, were retrieved from the
Otorhinolaryngic-Head & Neck Tumor Registry of the Armed Forces
Institute of Pathology. There was a 3.6:1 male/female ratio of patients
25-91 years of age (mean, 64.4 years). Patients presented most
frequently with hoarseness (n = 72 patients) present for a mean of 28.2
months. The majority of tumors involved the cricoid cartilage (n = 77)
with a mean size of 3.5 cm. All tumors were invasive and malignant by
radiology and/or histology (into bone within the ossified laryngeal
cartilages in 52 tumors). Most tumors were low-grade lesions: grade 1 (n
= 51), grade 2 (n = 54); there were six grade 3 tumors. An associated
benign chondroma with (n = 41 tumors) or without ischemia (n = 24
tumors) was noted. All patients had surgery and five had radiation
therapy. Wide excision or voice-sparing surgery was used in 73 patients,
whereas 37 patients had a laryngectomy. Recurrences occurred in 20 (18%)
patients, 10 of whom underwent salvage laryngectomy. At the last
follow-up, 102 patients had no evidence of disease (alive or dead, mean
11.2 years) and five patients had evidence of disease (alive, one
patient, 6.5 years; dead, four patients, mean 6.4 years). The six
patients with high-grade chondrosarcoma were all without disease at the
last follow-up (mean, 15.1 years). There was no difference in clinical
outcome based on grade (p = 0.210), location (p = 0.078), or treatment
(p = 0.607) but was worse for patients with a myxoid-type chondrosarcoma
(p = 0.044). Primary laryngeal chondrosarcomas are typically low- to
moderate-grade lesions involving the cricoid cartilage, frequently
associated with a chondroma. They usually portend an excellent overall
long-term prognosis with initial conservative voice-sparing surgery.
AU - Zoeteweij JP; Eyes ST; Orenstein JM; Kawamura T; Wu L; Chandran B;
Forghani B; Blauvelt A
Identification and rapid quantification of early- and late-lytic human
herpesvirus 8 infection in single cells by flow cytometric analysis:
characterization of antiherpesvirus agents.
SO - J Virol 1999 Jul;73(7):5894-902
AD - Dermatology Branch, National Cancer Institute,National Institutes of
Health, Bethesda, Maryland 20892, USA.
Human herpesvirus 8 (HHV-8) infection is associated with Kaposi's
sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's
disease. In this study, we used monoclonal antibodies (MAbs) directed
against HHV-8 lytic cycle-associated proteins encoded by open reading
frame (ORF) 59 (nuclear PF-8 protein) and ORF K8.1 (viral envelope
glycoprotein K8.1 [gpK8.1]) to investigate HHV-8 lytic infection in
single cells. Lytically infected cells were labeled with MAbs, stained
with fluorescently conjugated secondary Abs, and analyzed by flow
cytometry. A 3-day stimulation of HHV-8-positive PEL cell lines (BCBL-1
and BC-3) with 12-O-tetradecanoylphorbol-13-acetate (30 nM) or n-butyric
acid (0.3 mM) maximized the expression of lytic-phase viral proteins and
minimized cell toxicity. The absolute number of expressing cells was
inducer and cell line dependent. Expression of PF-8 occurred earlier and
more frequently (in up to 20% of cells) than did expression of gpK8.1. A
subset of PF-8 positive cells (25%) co-expressed gpK8.1, representing
the majority of gpK8.1 expressing cells. Acyclovir, foscarnet,
cidofovir, and PMEA reduced the number of cells expressing gpK8.1, but
not the number expressing the nonstructural early lytic gene product
PF-8. By contrast, alpha interferon (IFN-alpha) and IFN-beta reduced
expression of both PF-8 and gpK8.1, implying an overall inhibitory
effect on viral gene transcription or translation. In summary, we have
characterized and quantified HHV-8 lytic infection in single cells by
dual measurement of early- and late-lytic-cycle HHV-8 protein
expression. This technique should prove useful for screening of possible
antiherpesvirus agents and for detailed phenotypic characterization of
HHV-8-infected cells in vitro and in patients with HHV-8-associated
AU - Wang QJ; Jenkins FJ; Jacobson LP; Kingsley LA; Day RD; Zhang ZW; Meng
YX; Pellett PE; Kousoulas KG; Baghian A; Rinaldo CR Jr; Pellet PE
Primary human herpesvirus 8 infection generates a broadly specific
CD8(+) T-cell response to viral lytic cycle proteins.
SO - Blood 2001 Apr 15;97(8):2366-73
AD - Department of Infectious Diseases and Microbiology, Graduate School of
Public Health, University of Pittsburgh, Pennsylvania 15261, USA.
Human herpesvirus 8 (HHV-8) is a recently discovered gammaherpesvirus
that is the etiologic agent of Kaposi sarcoma (KS). The natural history
of primary HHV-8 infection, including clinical outcome and host immune
responses that may be important in preventing disease related to HHV-8,
has not been elucidated. The present study characterized the clinical,
immunologic, and virologic parameters of primary HHV-8 infection in 5
cases detected during a 15-year longitudinal study of 108 human
immunodeficiency virus type 1 seronegative men in the Multicenter AIDS
Cohort Study. Primary HHV-8 infection was associated with mild,
nonspecific signs and symptoms of diarrhea, fatigue, localized rash, and
lymphadenopathy. There were no alterations in numbers of CD4(+) or
CD8(+) T cells or CD8(+) T-cell interferon gamma (IFN-gamma) production
to mitogen or nominal antigen. CD8(+) cytotoxic T-lymphocyte precursor
(CTLp) and IFN-gamma reactivity were detected during primary HHV-8
infection, with broad specificity to 5 lytic cycle proteins of HHV-8
encoded by open reading frame 8 (ORF 8; glycoprotein B homolog of
Epstein-Barr virus), ORF 22 (gH homolog), ORF 25 (major capsid protein
homolog), ORF 26 (a minor capsid protein homolog), or ORF 57 (an early
protein homolog), in association with increases in serum antibody titers
and appearance of HHV-8 DNA in blood mononuclear cells. CD8(+) T-cell
responses to HHV-8 decreased by 2 to 3 years after primary infection.
This antiviral T-cell response may control initial HHV-8 infection and
prevent development of disease.
AU - Davis DA; Rinderknecht AS; Zoeteweij JP; Aoki Y; Read-Connole EL; Tosato
G; Blauvelt A; Yarchoan R
Hypoxia induces lytic replication of Kaposi sarcoma-associated
SO - Blood 2001 May 15;97(10):3244-50
AD - HIV and AIDS Malignancy Branch, the Dermatology Branch, and the Medicine
Branch, National Cancer Institute, National Institutes of Health,
Bethesda, MD, USA. firstname.lastname@example.com
There is substantial evidence that Kaposi sarcoma-associated herpesvirus
(KSHV) plays an important role in the pathogenesis of all forms of
Kaposi sarcoma (KS). It has been noted that KS commonly occurs in
locations, such as the feet, where tissue may be poorly oxygenated. On
the basis of this observation, the potential role of hypoxia in the
reactivation of KSHV replication was explored by studying 2
KSHV-infected primary effusion lymphoma B-cell lines (BC-3 and BCBL-1)
latently infected with KSHV. Acute and chronic exposure of these cells
to hypoxia (1% O(2)) induced KSHV lytic replication, as indicated by an
increase in intracellular lytic protein expression and detection of
virus in cell supernatants by Western immunoblotting. In addition,
hypoxia increased the levels of secreted viral interleukin-6. Moreover,
hypoxia enhanced the lytic replication initiated by the viral inducer
12-O-tetradecanoylphorbol-13-acetate. Desferoxamine and cobalt chloride,
2 compounds that increase the intracellular levels of hypoxia-inducible
factor 1, were also able to induce KSHV lytic replication. These studies
suggest that hypoxia is an inducer of KSHV replication. This process may
play an important role in the pathogenesis of KS.
AU - Nishimura K; Ueda K; Sakakibara S; Ishikawa K; Chen J; Okuno T;
Yamanishi K
Functional analysis of Kaposi's sarcoma-associated herpesvirus RTA in an
RTA-depressed cell line.
SO - J Hum Virol 2001 Nov-Dec;4(6):296-305
AD - Department of Microbiology, Osaka University Medical School, Osaka,
OBJECTIVE: The viral transcriptional activator encoded by Kaposi's
sarcoma-associated herpesvirus (KSHV) open reading frame 50 (RTA) is
expressed in the immediate-early phase of reactivation. We isolated an
RTA-depressed cell line, BLS50-4, by subcloning from the KSHV-infected
cell line, BCBL-1. RESULTS: In addition to RTA, induction of some lytic
gene expressions was also remarkably reduced in BLS50-4 cells, but that
of the K8.1 and ORF65 gene expressions was not. Both the replication of
the KSHV genome and the release of KSHV DNA into the medium were greatly
reduced. Transfection of RTA into BLS50-4 cells restored the expression
of K9 (vIRF) and ORF59, but not K8.1. CONCLUSIONS: Thus, we showed that
expression of late genes was not directly controlled by RTA, unlike the
other groups concluded. We also showed that, by isolating the
RTA-depressed cell line, the RTA protein had a critical role in viral
DNA replication and the expression of several lytic genes.
AU - Nascimento AF; McMenamin ME; Fletcher CD
Liposarcomas/atypical lipomatous tumors of the oral cavity: a
clinicopathologic study of 23 cases.
SO - Ann Diagn Pathol 2002 Apr;6(2):83-93
AD - Department of Pathology, Brigham and Women's Hospital and Harvard
Medical School, Boston, MA 02115, USA.
Liposarcomas in the oral cavity have rarely been described, with less
than 50 reported cases to date and a purported predominance of the
myxoid type. We reviewed our experience with 23 atypical lipomatous
tumors/liposarcomas of the oral cavity. Twelve patients were men, 10
were women, and gender was not stated in one case. Age at presentation
ranged from 28 to 83 years (median, 49.5 years). The most commonly
affected site was the tongue and most cases presented as a slowly
growing, painless mass. The clinical impression was lipoma or fibroma in
the majority of cases. Tumor size ranged from 0.6 to 8.0 cm (median, 1.5
cm). Five cases were well circumscribed, 5 cases were focally
infiltrative, and 13 cases had markedly infiltrative margins. Twenty-one
cases were classified as atypical lipomatous tumors (of which 10 showed
spindle cell features), one as dedifferentiated liposarcoma, and one as
myxoid liposarcoma. Follow-up data was available in 13 of the 23 cases.
Five others were lost to follow-up after a short period. Eleven patients
remained free of disease without local recurrence or metastasis during
the period of follow-up that ranged from 10 months to 9 years (median,
24 months). Two patients had multiple local recurrences. Our study shows
that atypical lipomatous tumor is the most common type of malignant
fatty tumor to arise in the oral cavity with an apparently low risk of
recurrence if widely excised, although follow-up is relatively limited
thus far. Copyright 2002, Elsevier Science (USA). All rights reserved.
AU - Brownlee NA; Hazen-Martin DJ; Garvin AJ; Re GG
Functional and gene expression analysis of the p53 signaling pathway in
clear cell sarcoma of the kidney and congenital mesoblastic nephroma.
SO - Pediatr Dev Pathol 2002 May-Jun;5(3):257-68
AD - Department of Pathology and Laboratory Medicine, Medical University of
South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA.
Mutation of p53 has been implicated in progression of classical Wilms
tumor (WT) into the anaplastic variant (AWT), drug resistance, and poor
prognosis. Because of prognostic similarities, clear cell sarcoma of the
kidney (CCSK) has been classified with AWT and other aggressive
pediatric renal tumors, apart from congenital mesoblastic nephroma
(CMN), which is instead a relatively benign tumor of neonates.
Initially, CCSK and CMN were assumed to be ontologically related, but
the role of p53 in the pathogenesis of either disease has not been
sufficiently evaluated as in AWT. We examined the status of p53 in CMN
and CCSK in comparison to AWT by immunohistochemistry and mRNA analysis
of p53, the downstream effector p21(WAF-1/CIP-1) ( p21), the multidrug
resistance gene MDR-1, a putative target of p53, and the p53-antagonist
Mdm-2. Surprisingly, strong p53 nuclear immunoreactivity was found in
cultures from two CMN specimens, but not in frozen or fixed tumor tissue
from five other CMN specimens, nor in cell lines or tumor tissue from
CCSK. Sequence analysis excluded p53 mutations. The size of the p53 mRNA
in CMN and CCSK primary tumors excluded gross deletions or
rearrangements. Low levels of Mdm-2 mRNA in CCSK and CMN primary tumors
and cultures did not support a role for Mdm-2. Absence of MDR-1 mRNA
excluded MDR-1 in the drug-resistant phenotype of CCSK.
Cisplatin-induced p21 transactivation assays and G(1) cell cycle arrest
analyses showed that p21 transactivation and G(1) arrest occurred in
both CCSK and CMN cultures, demonstrating integrity of the p53 signal
transduction pathway. Absence of p53 functional abnormalities excluded
relationships between CCSK and CMN as in AWT, supporting the association
of cellular CMN with congenital fibrosarcomas as more recently proposed.
AU - Leuschner I; Heuer T; Harms D
Induction of drug resistance in human rhabdomyosarcoma cell lines is
associated with increased maturation: possible explanation for
differentiation in recurrences?
SO - Pediatr Dev Pathol 2002 May-Jun;5(3):276-82
AD - Kiel Paediatric Tumor Registry, Institute for Paediatric Pathology,
University of Kiel, Michaelisstrasse 11, D-24105 Kiel, Germany.
In rhabdomyosarcoma (RMS) of childhood and adolescence very little is
known about interactions of cytotoxic drugs and tumor cells. In
recurrent RMS the tumor cells are often more mature than in the primary
tumor. The biological properties of these cells are still a subject of
controversy. We investigated two human (RD2 and TE 671) cell lines by
cultivating them with doxorubicin, cisplatinum, and etoposide. Degree of
differentiation and proliferation rate were estimated morphologically
and by means of immunohistochemistry and a monolayer proliferation
assay. Both morphological and immunohistochemical maturation was
measurable in most resistant cell lines. An increase in myosin
expression was most marked in the etoposide- and doxorubicin-resistant
RD cell lines. The proliferation rate was decreased in almost all
resistant cell lines. Nevertheless, the resistant cell lines tolerated
high-dose levels of cytotoxic drugs at a higher proliferation rate than
parental cell lines cultivated under similar conditions. The maturation
seen in some recurrent tumors of RMS can be simulated in vitro by
cultivating cell lines with cytotoxic drugs at sublethal doses.
Interestingly, the resistance-associated induced maturation was not
accompanied by p170 expression. After comparing these in vitro results
with the maturation seen in RMS specimens after chemotherapy, we
conclude that chemotherapy-induced differentiation in vivo might be a
morphological sign of chemoresistance.
AU - Chen GK; Lacayo NJ; Duran GE; Wang Y; Bangs CD; Rea S; Kovacs M; Cherry
AM; Brown JM; Sikic BI
Preferential expression of a mutant allele of the amplified MDR1 (ABCB1)
gene in drug-resistant variants of a human sarcoma.
SO - Genes Chromosomes Cancer 2002 Aug;34(4):372-83
AD - Oncology Division, Department of Medicine, Stanford University School of
Medicine, 269 Campus Drive, Stanford, CA 94305-5151, USA.
Activation of the MDR1 (ABCB1) gene is a common event conferring
multidrug resistance (MDR) in human cancers. We investigated MDR1
activation in MDR variants of a human sarcoma line, some of which
express a mutant MDR1, which facilitated the study of allelic gene
expression. Structural alterations of MDR1, gene copy numbers, and
allelic expression were analyzed by cytogenetic karyotyping,
oligonucleotide hybridization, Southern blotting, polymerase chain
reaction, and DNA heteroduplex assays. Both chromosome 7 alterations and
several cytogenetic changes involving the 7q21 locus are associated with
the development of MDR in these sarcoma cells. Multistep-selected cells
and their revertants contain three- to six-fold MDR1 gene amplification
compared with that of the drug-sensitive parental cell line MES-SA and
single-step doxorubicin-selected mutants. MDR1 gene amplification
precedes the emergence of a mutant allele in cells that were coselected
with doxorubicin and a cyclosporin inhibitor of P-glycoprotein (P-gp).
Allele-specific oligonucleotide hybridization showed that the endogenous
mutant allele was present as a single copy, with multiple copies of the
normal allele. Reselection of revertant cells with doxorubicin in either
the presence or the absence of the P-gp inhibitor resulted in exclusive
reexpression of the mutant MDR1 allele, regardless of the presence of
multiple wild-type MDR1 alleles. These data provide new insights into
how multiple alleles are regulated in the amplicon of drug-resistant
cancer cells and indicate that increased expression of an amplified gene
can result from selective transcription of a single mutant allele of the
gene. Copyright Wiley-Liss, Inc.
AU - Actor B; Cobbers JM; Buschges R; Wolter M; Knobbe CB; Reifenberger G;
Weber RG
Comprehensive analysis of genomic alterations in gliosarcoma and its two
tissue components.
SO - Genes Chromosomes Cancer 2002 Aug;34(4):416-27
AD - Abteilung Molekulare Genetik, Deutsches Krebsforschungszentrum,
Heidelberg, Germany.
Gliosarcoma is a variant of glioblastoma multiforme characterized by two
components displaying gliomatous or sarcomatous differentiation. We
investigated 38 gliosarcomas for aberrations of tumor-suppressor genes
and proto-oncogenes that are commonly altered in glioblastomas.
Amplification of CDK4, MDM2, EGFR, and PDGFRA were found in 11% (4/35),
8% (3/38), 8% (3/38), and 3% (1/35) of the tumors, respectively. Nine of
38 gliosarcomas (24%) carried TP53 mutations. PTEN mutations were
identified in 45% (9/20) of the investigated tumors. Twenty gliosarcomas
were analyzed by comparative genomic hybridization (CGH). Chromosomal
imbalances commonly detected were gains on chromosomes 7 (15/20; 75%), X
(4/20; 20%), 9q, and 20q (3/20, 15% each); and losses on chromosomes 10
and 9p (7/20, 35% each), and 13q (3/20, 15%). Five different high-level
amplifications were mapped to 4q12-q21 (1 case), 6p21 (1 case), 7p12 (2
cases), proximal 12q (4 cases), and 14q32 (1 case) by CGH. Southern blot
and/or differential PCR analyses identified amplification of PDGFRA
(4q12), CCND3 (6p21), EGFR (7p12), CDK4 (12q14) and/or MDM2
(12q14.3-q15), and AKT1 (14q32.3) in the respective tumors. Separate
analysis of the gliomatous and sarcomatous components of eight
gliosarcomas by CGH after microdissection and universal DNA
amplification revealed that both components shared 57% of the
chromosomal imbalances detected. Taken together, our data indicate that
the genomic changes in gliosarcomas closely resemble those found in
glioblastomas. However, the number of chromosomes involved in imbalances
in gliosarcomas was significantly lower than that in glioblastomas,
indicating a higher genomic stability in gliosarcomas. In addition, we
provide further support for the hypothesis that the gliomatous and
sarcomatous components are derived from a single precursor cell clone,
which progressed into subclones with distinct morphological features
during tumor evolution. According to our data, gain/amplification of
genes on proximal 12q may facilitate the development of a sarcomatous
phenotype. Copyright 2002 Wiley-Liss, Inc.
AU - Mertens F; Stromberg U; Mandahl N; Cin PD; De Wever I; Fletcher CD;
Mitelman F; Rosai J; Rydholm A; Sciot R; Tallini G; Van Den Berghe H;
Vanni R; Willen H
Prognostically important chromosomal aberrations in soft tissue
sarcomas: a report of the Chromosomes and Morphology (CHAMP) Study
SO - Cancer Res 2002 Jul 15;62(14):3980-4
AD - Department of Clinical Genetics, Lund University Hospital, SE-221 85
Lund, Sweden. email@example.com
Cytogenetic analysis has not only provided important information on the
pathogenesis of soft tissue tumors but, by disclosing distinct
chromosomal rearrangements in different histopathological entities, has
also come to serve as a valuable diagnostic tool. Little is known as yet
about the potential prognostic impact of cytogenetic features detected
in these tumors. A total of 239 benign and 221 malignant soft tissue
tumors with clonal chromosome aberrations were subdivided according to
general karyotypic features, such as degree of complexity and ploidy
level, and rearrangements of specific chromosomal regions. The
cytogenetic variables were analyzed regarding clinical outcome, using
time to metastasis as the end point. Selected variables were then
compared with established clinicopathological predictors of metastasis
development. When the entire material was considered, 167 of 268
investigated cytogenetic variables were associated with clinical
outcome. Focusing on the subset of 151 patients with high-grade sarcoma,
17 variables were identified that, besides grade and size, were
associated with increased risk of metastasis development. A final Cox
regression analysis identified five independent cytogenetic predictors
of adverse outcome; breakpoints in chromosome regions 1p1, 1q4, 14q1,
and 17q2, and gain of regions 6p1/p2. An increasing effect on metastatic
risk was seen with increasing involvement of the selected cytogenetic
variables, even when different histopathological types were studied
separately. We conclude that cytogenetic data provide independent
prognostic information in soft tissue sarcomas. Furthermore, our results
point to specific areas of the genome harboring genes that may influence
the metastatic potential of sarcoma cells.
AU - Sato Y; Nabeta Y; Tsukahara T; Hirohashi Y; Syunsui R; Maeda A; Sahara
H; Ikeda H; Torigoe T; Ichimiya S; Wada T; Yamashita T; Hiraga H; Kawai
A; Ishii T; Araki N; Myoui A; Matsumoto S; Umeda T; Ishii S; Kawaguchi
S; Sato N
Detection and induction of CTLs specific for SYT-SSX-derived peptides in
HLA-A24(+) patients with synovial sarcoma.
SO - J Immunol 2002 Aug 1;169(3):1611-8
AD - Department of Orthopedic Surgery, Sapporo Medical University School of
Medicine, Japan.
To investigate the immunogenic property of peptides derived from the
synovial sarcoma-specific SYT-SSX fusion gene, we synthesized four
peptides according to the binding motif for HLA-A24. The peptides, SS391
(PYGYDQIMPK) and SS393 (GYDQIMPKK), were derived from the breakpoint of
SYT-SSX, and SS449a (AWTHRLRER) and SS449b (AWTHRLRERK) were from the
SSX region. These peptides were tested for their reactivity with CTL
precursors (CTLps) in 16 synovial sarcoma patients using HLA-A24/SYT-SSX
peptide tetramers and also for induction of specific CTLs from four
HLA-A24(+) synovial sarcoma patients. Tetramer analysis indicated that
the increased CTLp frequency to the SYT-SSX was associated with
pulmonary metastasis in synovial sarcoma patients (p < 0.03). CTLs were
induced from PBLs of two synovial sarcoma patients using the peptide
mixture of SS391 and SS393, which lysed HLA-A24(+) synovial sarcoma
cells expressing SYT-SSX as well as the peptide-pulsed target cells in
an HLA class I-restricted manner. These findings suggest that aberrantly
expressed SYT-SSX gene products have primed SYT-SSX-specific CTLps in
vivo and increased their frequency in synovial sarcoma patients. The
identification of SYT-SSX peptides may offer an opportunity to design
peptide-based immunotherapeutic approaches for HLA-A24(+) patients with
synovial sarcoma.
AU - Watanabe K
Leiomyosarcoma versus myofibrosarcoma.
SO - Am J Surg Pathol 2002 Mar;26(3):393-4; discussion 394-6
AU - Karakousis CP; Zografos GC
Radiation therapy for high grade soft tissue sarcomas of the extremities
treated with limb-preserving surgery.
SO - Eur J Surg Oncol 2002 Jun;28(4):431-6
AD - State University of New York at Buffalo, School of Medicine and
Biomedical Sciences, Kaleida Health, Millard Fillmore Gates Hospital,
Buffalo, NY, 14209, USA. email@example.com
AIM: It is common to use either pre- or post-operative radiation for
high grade sarcomas undergoing limb-conserving surgery. Since 1977, we
have adopted a selective policy of post-operative radiation, given only
in patients with inadequate surgical margins. METHODS: A retrospective
review of 114 patients (1977-1995) with high grade adult soft tissue
sarcomas of the extremities was carried out. Amputation was required in
10 (9%). Patients with a minimum surgical margin <2 cm (n=33) received
adjuvant radiation (29%). RESULTS: No complications occurred in 81/114.
Wound complications were infection (14%), seroma (6%), haematoma (4%),
dehiscence (4%) and skin edge necrosis (2%). Remedial operations were
required in four (3%). Overall, the local recurrence rate was 23/114.
Patients with surgery only (n=57) or surgery plus systemic chemotherapy
(n=24) manifested local recurrence in 15/81 (19%) and those with surgery
plus radiation (n=21) or surgery and radiation and chemotherapy (n=12)
suffered local recurrence in 8/33. The local recurrence rate for tumours
< or =5 cm was 6/32 and for those >5 cm 17/82, P=1.0. The 5 year
survival rate was 60% for tumours < or =5 cm (n=32) and 46% for tumours
> or =5 cm (n=82) (P=0.009). CONCLUSIONS: (1) Limb preservation was
feasible in 91% of patients. (2) When the local treatment modality was
surgery alone ('wide' margins) the local recurrence rate was 19%, and
when it was surgery plus radiation (narrow margins) it was 24%. (3)
Selective use of radiation (in patients with narrow margins) and
reliance on surgery alone in cases amenable to wide resection may be a
legitimate alternative to universal application of radiation with
conservative resection. Copyright 2002 Elsevier Science Ltd. All rights
AU - Behranwala KA; Clark MA; Thomas JM
Soft-tissue tumours of the perineum.
SO - Eur J Surg Oncol 2002 Jun;28(4):437-42
AD - Sarcoma and Melanoma Unit, Royal Marsden NHS Trust, London, UK.
AIM: Primary perineal tumours in adults are rare entities that have been
infrequently reported (one clinical series of nine patients with sarcoma
and isolated case reports). We present our experience of perineal
tumours better to characterize and define the natural history of this
condition. METHODS: Perineal tumours occurring in adults (>18 years)
Sarcoma Unit prospective database. RESULTS: Nineteen perineal
soft-tissue tumours (12 malignant and seven benign) were evaluated and
treated at the Royal Marsden Hospital during this period. Liposarcoma
(n=3) and aggressive angiomyxoma (n=4) were the most frequent
histological subtypes. Three malignant and four benign tumours were
larger than 10 cm. Most tumours were located deep to the deep fascia.
Local recurrence in those with sarcoma occurred in one of five patients
with negative microscopic margins and in one of two patients who had
positive microscopic margins. One patient each with aggressive
angiomyxoma and fibromatosis recurred locally. CONCLUSION: Aggressive
treatment in the form of wide local excision is associated with fewer
local recurrences in adults with primary perineal soft-tissue tumours.
The aim of surgical treatment should be to obtain negative resection
margins without causing disturbance to urinary or anorectal function.
AU - Odone-Filho V; Cristofani LM; Bonassa EA; Braga PE; Eluf-Neto J
In vitro fertilization and childhood cancer.
SO - J Pediatr Hematol Oncol 2002 Jun-Jul;24(5):421-2
AU - Estourgie SH; Nielsen GP; Ott MJ
Metastatic patterns of extremity myxoid liposarcoma and their outcome.
SO - J Surg Oncol 2002 Jun;80(2):89-93
AD - School of Medicine, University of Utrecht, Utrecht, The Netherlands.
BACKGROUND AND OBJECTIVES: Extremity myxoid liposarcomas have a unique
extrapulmonary metastatic potential. We studied the metastatic pattern
of extremity liposarcomas to determine what types of posttreatment
imaging may be of value in the follow-up these patients. METHODS:
Twenty-two patients from a total of 128 patients with primary extremity
liposarcoma were treated at a tertiary care institution for subsequent
months (range: 6-270 months). Data on these patients was prospectively
collected and then retrospectively analyzed for effect of metastatic
pattern and treatment on outcome. RESULTS: Of these 22 patients,
extrapulmonary metastases developed in 10, combined pulmonary and
extrapulmonary metastases developed in 6, and isolated pulmonary
metastases developed in 6. Of the 16 patients with extrapulmonary
metastases, 13 were of the myxoid subtype. Of the 49 patients with
extremity myxoid liposarcomas, metastases developed in 14 (29%). The
most common sites of metastases among these 14 patients include: the
retroperitoneum, 10 patients (71)%; intra-abdominal extra-hepatic, 7
patients (50%); spinal/paraspinal, 6 patients (43%). Only 3 of the
patients are alive and disease free and all 3 of these patients are from
the subgroup of 10 patients with only extra-pulmonary metastases (2
intra-abdominal and 1 retroperitoneal). CONCLUSIONS: Extremity myxoid
liposarcomas have an unusually high predilection for extra-pulmonary
metastases, frequently without any pulmonary metastases. After treatment
of the primary tumor, these patients should be followed with periodic
chest X-ray and abdominal/pelvic computed tomography (CT) scans. Any
back or neurologic complaints should prompt additional imaging of the
appropriate spinal area. Consideration should be given to surgical and
adjuvant treatment of metastatic disease when appropriate.
AU - Pisters PW; Sondack VK
Metastatic patterns of extremity liposarcoma and their outcome.
SO - J Surg Oncol 2002 Jun;80(2):94-5
AD - Sarcoma Service, M. D. Anderson Cancer Center, Houston, Texas, USA.
AU - Drabick JJ; Davis BJ; Lichy JH; Flynn J; Byrd JC
Human herpesvirus 8 genome is not found in whole bone marrow core biopsy
specimens of patients with plasma cell dyscrasias.
SO - Ann Hematol 2002 Jun;81(6):304-7
AD - Hematology/Oncology Service, Walter Reed Army Medical Center,
Washington, DC 20307-5000, USA. firstname.lastname@example.com
Recently, molecular evidence of the gamma herpesvirus, human herpesvirus
8 (HHV-8), was found in the nonmalignant bone marrow stromal cells of
patients with multiple myeloma using a polymerase chain reaction
(PCR)-based assay. Other investigators have been unable to confirm
either the presence of HHV-8 using molecular techniques or serologic
evidence of prior infection with HHV-8. In order to maximize the
likelihood of detection of small quantities of the virus and minimize
the risk of potential nucleic acid contamination, we used entire bone
marrow biopsy core specimens for DNA extraction and amplification. These
specimens included both malignant plasma cells and bone marrow stromal
cells and were subjected to minimal manipulation prior to DNA extraction
and PCR. We tested eight patients with various plasma cell dyscrasias
and compared them to negative controls with non-Hodgkin's lymphoma using
standard PCR assays utilizing the KS330(233)primers and probe for HHV-8.
This assay is reproducibly positive in Kaposi's sarcoma tissue. We found
no evidence of HHV-8 DNA in either the lymphoma controls or the samples
from patients with the plasma cell dyscrasias using these methods. We
conclude that HHV-8 is unlikely to play a major role in the pathogenesis
of the plasma cell dyscrasias in the majority of patients with these
diseases. This report adds to the body of evidence that HHV-8 is not
associated with plasma cell dyscrasias like multiple myeloma.
AU - Murta EF; Oliveira GP; Prado Fde O; De Souza MA; Murta BM; Adad SJ
Association of uterine leiomyoma and Chagas' disease.
SO - Am J Trop Med Hyg 2002 Mar;66(3):321-4
AD - Discipline of Gynecology and Obstetrics, Faculty of Medicine of
Triangulo Mineiro, Uberaba, Minas Gerais, Brazil.
With the aim of studying the frequency of Chagas' disease among
sufferers of uterine leiomyoma, we analyzed women older than 35 years
who underwent surgery and presented with leiomyoma on
anatomicopathological examination. The diagnosis of Chagas infection was
based on positivity to at least two of three serological tests:
enzyme-linked immunosorbent assay, passive hemagglutination, and
immunofluorescence. The study was case controlled, matching for age,
skin color, and parity. The control group consisted of women undergoing
surgery for other benign gynecological alterations. During this period,
118 women presented with uterine leiomyoma, 27.1% of whom were
serologically positive for Chagas' disease versus 16.1% of the controls
(P < 0.05). Matching by skin color and parity showed that 40% of the
white multiparous women with uterine leiomyoma had Chagas' disease
versus 10% of the controls (P < 0.05). We concluded that there appears
to be an association between Chagas' disease and uterine leiomyoma.
AU - Sandberg A; Bridge J
Updates on the cytogenetics and molecular genetics of bone and soft
tissue tumors: alveolar soft part sarcoma.
SO - Cancer Genet Cytogenet 2002 Jul 1;136(1):1-9
AD - Department of DNA Diagnostics, St. Joseph's Hospital and Medical Center,
350 West Thomas Road, Phoenix, AZ 85013,USA.
AU - Rapp TB; Yang L; Conrad EU 3rd; Mandahl N; Chansky HA
RNA splicing mediated by YB-1 is inhibit


text:
From Oct. 4-7 MUFSO 2009 will celebrate its golden anniversary, marking five decades of Multiunit Foodservice Operators conferences. The upcoming MUFSO in Dallas truly will be a celebration of this important industry event. But more important it will be a celebration of an industry that has come of age during those 50 years.The first year MUFSO was held, 1959, a number of key events occurred. President Dwight D. Eisenhower signed a proclamation in January admitting Alaska as the 49th state, ...
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subset: commoncrawl


text:
Milwaukee's cheesiest shop, bar and cafe
Have a cow, man. Why not? After all, it's Dairy Month here at OnMilwaukee.com. Join us all month long as we explore all things that make you go "Moo" and celebrate America's Dairyland during the Dairy Days of Summer! Brought to you by OnMilwaukee.com and the Wisconsin Milk Marketing Board.
For most of its 74 years, the Wisconsin Cheese Mart was a sleepy little shop on Old World Third Street. Originally in the old Metropolitan Block building, across an alley from Usinger's, the store moved a half block north in 1976 after its historic edifice burned.
The mart ran through a succession of owners, and at times seemed to be as much a deli as a cheese retailer. Enter Ken McNulty in 2003.
A software implementer who spent too much time on the road, he had tired of living out of a suitcase, and he wanted to own a business that could sell its products online. McNulty bought the Wisconsin Cheese Mart and it woke up.
The new owner swept all of the non-Wisconsin cheeses, except a handful of European specialties, off the shelves. He installed a high-powered website that offers customers multiple ways to navigate through the shop's vast inventory.
Cheese categories include "stinky," "spicy" and "smoked." You can browse through cheeses that have won awards, are made in small batches, or are in unusual shapes. Internet sales leaped 1,000%.
The mart ships to all 50 states from an old cigar factory located a block north and across the street from the retail space. California, Texas and Florida are the three hungriest states for Wisconsin Cheese Mart products.
McNulty gave the bricks and mortar location a merchandising facelift, and three years ago he expanded into the adjoining storefront, adding a full bar and cafe open to midnight on weekends. Shop sales have increased 600% since he bought the business.
Cheese plates ($7.25 to $11.25) for pairings with wine and beer are offered. Deli plates of sausage ($6.75), meat and cheese ($7.75) and charcuterie ($12) are joined on the menu by a half dozen variations on grilled cheese sandwiches ($3.50 to $8.25), wieners and brats on pretzel buns ($4.50), and of course, cheesecake ($3.75).
The sausages are from Usinger's, Bolzano, and Wautoma's StoneRidge Meats.
All of the bottled and tap beer is brewed in Wisconsin, with the selection ranging from Miller and Leinie's to Capital and Furthermore. Beer and wine flights are available.
McNulty expected most customers to pair wine with their cheese, but ya der hey, this is Wisconsin. Brewskis rule. Beer decisively outsells wine, and the owner responded by bulking up the beer list and selling six-packs to go.
The bar and cafe contribute only a small number to the business' bottom line, according to McNulty, but they drive store cheese sales, which in turn drive internet sales. Customers can wander the shop with a glass of beer or wine in hand. That encourages browsing and lowers reluctance to trying something new.
The mart stocks more than 200 Wisconsin cheeses, and many are unusual. Basic aged cheddars are the best sellers in the shop and online, but check out some of these more exotic offerings:
- Mango Fire Heritage Cheddar from Henning's Wisconsin Cheese in Kiel uses mango and habanero peppers to give your mouth a pop. An 8-oz. package is priced at $6.25.
- Raspberry BellaVitano is a cheddar and parmesan mix soaked in New Glarus Brewing Company's Raspberry Tart beer. The winner of the 2008 World Cheese Award for best new cheese and best American cheese, it is made by Plymouth's Sartori Cheese. The Raspberry BellaVitano sells for $4.65 for 5.3 ounces.
- Nut Brown Ale Caerphilly, a cave-aged Welsh miner's cheese soaked in Bull Falls Brewery ale, is made by Harmony Specialty Dairy Foods in Stratford. It is $17.75 a pound.
- Wild morel mushroom and Jack cheese from the Meister Cheese Company in Muscoda costs $4.45 for 8 ounces.
- Juusto, a Finnish-style baked cheese served heated with jelly or jam, is produced by Pasture Pride Cheese in Cashton. It is $4.95 for 7 ounces.
McNulty said the cheese industry is mirroring the brewing business in the splintering of the market among specialty and niche products. Industry growth is not coming from the familiar lowest common denominator brands, but from cheese makers offering new and intriguing flavors.
The Wisconsin Cheese Mart attracts a substantial number of tourists seeking a slice of Milwaukee's heritage on Old World Third Street, and McNulty uses that introduction to make them follow-up customers online. "It helps to have a store and not just a virtual presence," he said.
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Meet the Team
Julie Meyer
Chairman and Chief Executive - Ariadne Capital; Managing Partner – Ariadne Fund; Founder – Entrepreneur Country; MBE
Julie Meyer founded Ariadne Capital in August 2000 to create a new model for the financing of entrepreneurship in Europe and the UK - "Entrepreneurs Backing Entrepreneurs". She created the founding Investor Partnership of 60 leading entrepreneurs and business builders who are Ariadne's core shareholders.
She has led or co-led the investments and/or advisory mandates with BeatThatQuote, Espotting, Monitise, SpinVox, and Zopa. She manages the Ariadne fund, sitting on the Boards of Clickslide, Taggstar and itrigga.
Julie was part of the early team at Tom Teichman's NewMedia Investors in 1998, which became early stage investment firm Spark Ventures in October 1999. At NewMedia Investors, she co-managed deals in key technology companies including ArcCores, lastminute.com and WGSN. Also, in 1998, she founded First Tuesday, the network of entrepreneurs, which many credit for igniting the Internet generation in Europe. It was sold in July 2000 for $50 million in cash and shares.
She has been named one of INSEAD's Top 50 Alumni, Ernst & Young Entrepreneur of the Year, World Economic Forum Global Leader of Tomorrow, TIME Magazine Digital 50, one of WIRED's 100, one of London's 1000 Most Influential People (Evening Standard) and one of the Top 30 Most Influential Women in Europe.
Julie is also one of the BBC's Online Dragons in the award-winning Dragon's Den Online, and is also currently setting up a "Dads and Daughters" foundation to support and extend her thesis that women's identities are shaped - for good or bad - by the messages they receive from their father's as children and young adults.
She is on the Board of Directors of Medikidz, Vestergaard Frandsen, a for profit humanitarian development firm, and INSEAD, her business school. She graduated with a Bachelor of Arts degree in Humanities and English Literature from Valparaiso University, near Chicago, US in 1988, having spent her junior year in Cambridge, UK in 1986.
In 2011, she was asked to be on the Secretary of State's Entrepreneurs Panel and the Secretary of Health's Innovation Panel. In 2012, Julie was honoured to receive an honorary MBE, for her 'services to entrepreneurship'. Julie's first book 'Welcome to Entrepreneur Country' was published in June 2012.
To schedule a call or an appointment, contact Jessica Gardner on firstname.lastname@example.com.
For speaking opportunities please contact Jan McGinley - email@example.com.
Julie Meyer, MBE
Ariadne Capital – CEO – in our 13th year
Managing Partner – Ariadne Fund
Entrepreneur Country – Founder
+44 203 021 1641
Amit Pau
Director - Corporate Finance and Entrepreneur Country
Amit is deeply passionate about helping companies exploit disruption (business models and technology) to transform their business. His 20+ years experience in the TMT industry has given a robust platform to launch numerous market leading solutions in areas such as enterprise mobility, mobile entertainment, managed data services, digital marketing and electronic commerce. Whether it is in a corporate leadership role or an entrepreneurial environment Amit consistently drives innovative 'go to' market strategies in order to deliver quantum growth.
In his previous high profile corporate positions at Vodafone, as Group MD for International Accounts and Business Markets, Amit was instrumental in creating strategic OEM partnerships with Dell, IBM and HP. Additionally, he led the Divisional Units of AT&T and GTS where he launched their e-commerce products and delivered significant scale.
Amit has transformed multiple entrepreneurial companies; whether it be as an investor driving the IPO's or NED working with the Executive team in refining their business models, mentoring them with scaling their operations in order for successful exits. This vastly enriching experience has enhanced his experience in commercialising innovation with a combination of business models and disruptive technologies.
Over the years Amit has served on a number of listed and private boards such as Vodafone Spain, Radamec Plc, IOS Plc and Goldnet, to name a few.
Lorna Collings
Senior Advisor – Fund Administration
Lorna Collings has worked in the Private Equity industry for 15 years starting her career with BancBoston Capital where she held positions in both the Finance Department and the Investment Team. After five years she moved to CDC Group plc to work on the spin out of Actis Capital LLP. Subsequently spent six years with Gartmore Private Equity and was an integral part of the project team responsible for the complex joint venture with Hermes Private Equity to create Hermes GPE LLP, where she was Finance Director. More recently she works as a consultant, advising Finance Teams in the Private Equity & Venture Capital industry.
Lorna has a BA (Hons) degree in Applied Psychology from Sussex University and became a Chartered Management Accountant, qualifing in 2000.
Paul Burniston
Senior Advisor - Finance
Paul has 25 years experience in corporate strategy, visioning and driving strategic change, innovation and performance improvement throughout an organisation be that organically or through transactions. Extensive experience building businesses in retail financial services and online media sectors. At Interactive Investor as Head of Corporate Strategy and Development, Paul led the development of the online brokerage proposition, growing it from start-up to one of the leading online brokers in UK, managed the finances leading the business into profit, and led capital raisings and acquisitions.
Prior to Interactive Investor, Paul worked for 5 years at AMP, setting up an online fund supermarket business from concept to implementation, and developing and executing their European entry strategy.
Paul started his career with 10 years in management consultancy at OASiS and Ernst & Young delivering large scale business transformation programmes across people, process and systems.
Jessica Gardner
Executive Assistant to CEO
Jessica joined Ariadne Capital in April 2013 as the Executive Assistant to CEO, Julie Meyer.
Prior to Joining Ariadne Capital, Jessica was an Executive Assistant at GE Capital EMEA Services Ltd where she provided support to two Senior Leaders.
Jessica has 8 years PA/EA experience in Financial Services and previous experience in Marketing and Event Management within the leisure industry.
Peter Hale
Finance Manager
Peter graduated in 2012 with a BA (Hons) in Business Management and Accounting & Finance degree from Nottingham Business School, and whilst at University was actively involved in the 1stXV Rugby team and Business and Finance Society.
He is currently undertaking his Professional Accountancy qualifications (ACCA) and before working at Ariadne gained experience working at a Chartered Accountancy Firm.
A keen sportsman and traveller, Peter enjoys playing everything from Rugby through to Croquet and has spent time in places such as Southern Africa, Australia & New Zealand, South East Asia and Europe.
Claire Langmead
Executive Assistant
Claire joined Ariadne Capital in October 2010, and is the Executive Assistant to CEO, Julie Meyer.
Prior to joining Ariadne Capital Claire was Team Secretary at Prudential Property Investment Managers, the property investment arm of M&G Investments, where she provided support to two Fund Managers & six Investment Managers.
Claire also previously worked with the event management industry, and has several years experience as part of an events team working on many promotional marketing campaigns for large companies such as Lexus, Toyota, O2, & T-Mobile. She also has many years experience within the entertainment industry as a dancer and singer. Claire has peformed in several theatre shows and spent 7 years touring theatres around the UK as, she has also appeared as a Background Artiste in TV & films.
James McClurg
Assistant Director, Ariadne Capital & Investment Manager ACE Fund
Before joining Ariadne Capital James was a consultant in the Strategy practice at Deloitte London. During this period James worked with both large corporates and entrepreneurial businesses on pure strategy projects and transaction based projects.
James has a BA (Hons) in Business Economics and MSc Management with Distinction. Whilst at university and since leaving university, as a personal pursuit, James has started and assisted in the creation of a number of small businesses.
Ian Findlay
Director of M&A and Corporate Advisory
Ian Findlay has 20+ years experience working in strategy formulation and transaction initiation, structuring and execution.
At Ariadne, Ian has been engaged by a variety of start-ups to advise on their business development and funding, and also by corporates looking to embrace digital business models. Ian has also assisted numerous start-ups exit successfully, including being the sole financial adviser on the sale of comparison site BeatThatQuote to Google at 122xEBITDA, and the sale of social media agency Simply Zesty to UTV Media plc. Ian has also advised on the buy side and assisted ITV in their acquisition review of Channel 5, and advised of the purchase from Reed Elsevier of Travel Weekly and Caterer & Hotelkeeper.
Some of the mandates completed by Ian at Ariadne are shown in the tombstones below.
Ian is continuing to build Ariadne's corporate finance presence, covering M&A (Sell-side and Buy-side), valuation and raising capital, together with strategy development and corporate advisory.
Ian spent 16 years with Emap, during the last six of which he was Director of Corporate Development and latterly a member of the Executive Board. Ian has an extensive track record of completing transactions including partnerships, joint ventures, acquisitions and divestments, and is a seasoned negotiator. At Emap Ian was responsible for 47 completed M&A transactions with a total value of £4.5 billion - 32 acquisitions and 15 disposals.
Prior to joining Emap in 1992, Ian was Business Development Director of RBPG, a division of Reed Elsevier, where he completed numerous transactions.
Ian is MBA and degree qualified, and has worked in the USA, France, Germany, Netherlands, Spain, Italy and the Middle East.
Nicolas Perard
Nicolas Perard joined Ariadne Capital in July 2012 and focuses on supporting the Ariadne Capital Entrepreneur (ACE) fund. Before joining Ariadne, Nicolas had previous experiences in Venture Capital at DN Capital and Management Consulting at Fiserv.
Passionate about early and growth stage tech investments and entrepreneurship Nicolas completed a Master Degree in Technology and Management from École Centrale Paris after graduating from Paris Dauphine University with a Master Degree in Finance.
Jonathan Simnett
Senior Adviser, Ariadne Capital
Jonathan Simnett has nearly 30 years' experience, winning in excess of three dozen awards, building businesses and helping public and private sector organisations and their investors in fast-growth B2B and B2C segments to: grow; manage change: reposition; enter new markets; acquire; float and report through the delivery of more effective management and marketing communications strategies.
Leader of the team named Global Communications Department of the Year at the 2009 International Business Awards, New York, he was formerly vice chairman in the UK of Brodeur Worldwide, a global public relations consultancy. He was part of the team that founded and built a company of 650 people and $95 million turnover worldwide over a period of 15 years, before selling to Omnicom. He also sat on the board of Brodeur in Europe and became one of the UK's first Investor in People.
He has worked with established companies in periods of rapid change such as: AT&T; Avaya; BT; C&W; Cisco; HP; Huawei; Infosys; Intel; Myriad; Novell; Informix; IBM; Nortel Networks; OpenReach; TCS; Time Out Group and Sybase. In addition, he has also worked with fast growth or venture-backed companies taking many to sale or public offering including: Blast Radius; Calyx; Civica; Concateno; Centiq; Clear; e2v technologies; Gear4; Getjar; Global Graphics; Holition; Intuwave; Interoute; KeyPoint Technologies; Technis; marketingQED; SpinVox; WEEE Systems, Westhouse Medical and Zomm.
He has also delivered campaigns for CEOs and financiers and advisory firms such as Ariadne Capital and Battery Ventures and their portfolios and for government organisations such as: European Commission DG XIII; Innovation Norway; Locate in Scotland; Royal Norwegian Embassy; Salford Regeneration and Thames Valley Enterprise. In addition, he's worked with the management teams of agencies including: Chameleon; CognitoMedia; Komodo; Fleishman-Hillard; Midnight Communications; Positive Marketing and Rochester PR Group.
Linda Peters
Senior Advisor, Ariadne Capital - Luxury Retail, Fashion
Linda has a diverse background with experience in entrepreneurial ventures, corporate venturing with major global companies, and business model innovation that spans over 25 years, including over 12 years in the Luxury & Lifestyle industry sector. Her unique background combines luxury e-commerce expertise, along with experience in more traditional retail platforms for developing brands and luxury businesses. Linda has held leadership and operating roles in the industry as CEO, MD, and VP Global Strategy, Business Development and Strategic Marketing. Her experience includes: overall leadership and management of early and growth stage businesses with full P&L responsibility, strategy development, strategic marketing and operations management.
She has particular experience in developing and growing luxury fashion & beauty and lifestyle businesses. At Sephora.com (owned by LVMH), one of the top leaders in online luxury beauty retailing, she was responsible for global strategy, business development, and strategic marketing where she built strategic partnerships and led marketing initiatives that resulted in significant growth of the customer base and revenue. Recently, she was Interim MD for Cocosa.com, a UK flash-sales fashion business owned by Bauer Media, where under her leadership Cocosa achieved high growth and a platform for sustainable growth. She developed and was executing on a new lifestyle strategy including Travel and Beauty and International expansion. She was instrumental in the successful sale of Cocosa to Al Fayed Family Trusts completed in July 2011.
Upon moving to London from San Francisco in 2002, in collaboration with the University of Arts London, she co-founded the Centre for Fashion Enterprise, a successful and innovative venture programme for British Fashion start-up businesses. In 2006, she joined a UK/US based private equity advisory firm where she was the industry CEO for the Luxury Fashion & Beauty sector.
Prior to working in the luxury industry, she held senior/partner-level positions in prestigious US consulting firms including CSC Index based in Cambridge MA, and Strategos (under Gary Hamel's leadership) based in Silicon Valley, California. She consulted to corporations on strategy innovation, corporate venturing, and design of new operating models.
She has board-level experience as a Non-executive Director and Company Secretary. Educated in the US, Linda holds a Bachelors of Science degree in Computing Science with honours from the University of Evansville. Linda is a dual British and American citizen and resides in London.
Melinda Nicci
Senior Advisor for Health Technology, Ariadne Capital
Melinda Nicci MSc, is a Sport Psychologist and a successful international entrepreneur and consultant in the health and wellbeing industry. As well as being an expert advisor and speaker, Melinda held the position of Senior Director in Global Group Innovation, at Philips Head Office in Amsterdam. She is currently Senior Advisor for Health Technology for Ariadne Capital. Based in London, Melinda also acts as a mentor and advisor on accelerator programmes in the technology sector, as an innovation mentor for executives, and consults to companies from blue chip to start up, including the NHS, in developing technology based health propositions; strategy; go- to- market; partnerships and business models. Melinda has also co-written a book, produced a very successful wellbeing DVD, has written for many publications, and has had numerous television appearances as a health expert. Melinda developed a new health and wellbeing educational initiative for teenagers, which is piloting in schools in London.
Kit Ruparel
CTO in Residence
Kit is a board experienced CTO and CIO with a skill-set across software development, IT systems and company operations.
The majority of his career has been spent growing start-ups or turning around SMEs that may otherwise have been regarded as 'end-of-life'.
Having mostly worked for private-equity or VC backed organisations, Kit's skills have been regularly used by investors to evaluate companies they may have an interest in, perform due-diligence activities, or assist with growth or turnaround strategies.
With an exceptional ability to rapidly understand a new product or service, the business model around it and whether the technology and operations are helping or hindering the company vision, Kit is as comfortable operating within experienced boards as he is probing lines of source-code with the developers and engineers.
Extremely innovative, Kit rapidly finds solutions to any technical or operational challenge; and as well as having patents to his own name is also excellent at identifying the unique product features or methods in others' intellectual property and then making the most of them from a business perspective.
Kit's network and access to skills is very broad, having worked with experts across virtually every technological discipline, using development and BPO organisations across most continents, peering with 'deep' contacts (i.e. true technology owners, not evangelists) inside organisations like Microsoft, and negotiating products, services and partnerships from the largest name-brand companies down to the smallest specialist suppliers.
Kit is a Chartered IT Professional and has voluntarily assisted the British Computer Society, the National Computer Centre, the DTI, the Learning and Skills Council and the government e-Skills programme on a range of topics from Professionalism in IT initiatives to defining skills frameworks for CIOs through to IT end-users.
Kelly Dolan
Head of Editorial - Entrepreneur Country
Kelly Dolan joined Entrepreneur Country in January 2012 as Head of Content.
Her current responsibilities include managing Entrepreneur Country's host of media platforms, including creating a weekly newsletter for investors and entrepreneurs, designing and editing a monthly digital magazine as well as managing an inventory of 200 leading entrepreneurial contributors, who share their business insights with Entrepreneur Country readers through it's various communication channels.
Kelly has also worked on account management, business development and sales, as well as strengthened engagement and increased the population of the Entrepreneur Country community.
Prior to joining Entrepreneur Country Kelly worked as a freelance journalist for Bauer Media on a range of men's and women's titles during crucial periods of digital transformation. Kelly also speaks at young entrepreneurial programmes, workshops and business schools, as well as sits on panels for industry events.
Paul Macavoy
Senior Advisor Talent
Paul has over 16 years experience in the Executive Search and Talent Advisory market. His core areas of expertise include Talent Acquisition, Management and Development, with a particular focus on the people dynamics that effect start-ups and high growth businesses experiencing change.
The majority of Paul's experience is within the Technology, Telecommunications and Media Sectors and includes extensive work with early stage start-ups as well as FTSE 100's and leading Professional Services firms.
He is currently advisor to a number of CEO's in the TMT market and works closely with them to create dynamic and effective leadership teams.
Jan McGinley
Speaking Agent
Jan joined Ariadne in December 2012 to manage Julie Meyer's global speaking engagements, bringing a wealth of experience in PR, project management and business development. Having started her first business at the age of 16, Jan has continued to build small companies over a number of years, with her experience as an entrepreneur giving her a measured and intuitive approach to her speaking agent role, supported by a huge network of contacts built through her 20 years in business.
Prior to joining Ariadne, Jan spent 5 years in PR, during which time she set up an Entrepreneur Ambassador Panel for a Government project, media trained business experts and worked with a number of corporates and well known high street brands.
Jan has a BA Honours from UEL. She has travelled extensively in South America, India and Australia.
Steve Piper
Financial Controller
Previously employed by Sea Containers Ltd for 29 years reaching the position of Group Chief Accountant responsible for producing and filing the consolidated accounts of both Sea Containers and Orient Express Hotels Ltd . A hard working individual with experience within the leisure activities (hotels & train), publishing, travel industry, container leasing, manufacturing, shipping and property development.
Steve O'Donnell
Senior Advisor
Steve O'Donnell has over 30 years global experience working at C-level in large multinational organisations. He was CEO at MEEZA, a Qatar Foundation Joint Venture, Managing Director at ESG, Global Head of Data Centre Operations at BT and has held senior positions at Cable & Wireless, Lehman Brothers, and Deutsche Bank.
He is globally recognised as an expert in green IT and sustainability having won six industry awards including the British Computer Society best use of green technology award in 2007. He is an active angel investor and Non Executive, working in Smart Grid, Sustainability, Data Centre cooling technology, IT Security and the IT Services sector. He is an inventor and holds a number of patents in the data center cooling area. In 1999 one of the companies he led as CEO won a Fast 50 special award from Deloitte & Touche for rapid company growth achieving over 3000% in three years.
Steve is a entrepreneur, and a global businessman, with a deep understanding of how to drive performance and efficiency in the organisations he manages. He has successful experience in six trade sales, two MBOs and an IPO. He is an active participant in social media with a loyal following on his blog http://www.thehotaisle.com and a growing audience on Twitter @stephenodonnell. He likes to add value in analysing and understanding the problem, identifying the customer segments and honing the message and product offering to help drive sales execution.
Tobias Straessle
Senior Advisor
Tobias Straessle is an internationally experienced senior business executive with over 30 years in the financial services industry as line manager and management consultant. He has a track record of shaping & implementing new technology-based business operating models & services, scaling businesses for growth, managing complex business & organizational change programs, optimizing value chains for higher productivity and better cost efficiency, building & leading large multi-cultural, high-performance IT and Operations teams.
His expertise includes business and technology innovations in capital markets trading & settlement, wealth management and asset management, service outsourcing/insourcing, technology due diligence, people development and coaching, management and strategic leadership of operations & information technology departments.
Tobias Straessle is today engaged on various fronts: He owns Grey Cells Associates Ltd, a Management & Technology Consulting firm; he is a Senior Advisor of Eleven Canterbury Ltd., a firm that coaches small to mid-sized technology-based companies for Financial Services market entry; he is Chairman of Saxo Bank (Switzerland), the subsidiary of a global investment bank offering online capital markets trading and investing for retail, professionals including White Label partners. Previously Tobias spent over three years with Saxo Bank Group, as COO and CIO, based in Copenhagen, 15 years with UBS/SBC Investment bank in London & Zurich, one year with CBI in Geneva, seven years with Andersen Consulting and a few years with Credit Suisse. Tobias holds a Master Degree in Mathematics from the Swiss Federal Institute of Technology (ETHZ) and an MBA from J.L.Kellogg School of Management at Northwestern University in Chicago. He speaks English, German and French
Rhea Sethi
Head of Events - Entrepreneur Country
Rhea Sethi joined the Entrepreneur Country team in 2012 to manage its extensive events portfolio, as well as support business development.
As Head of Events, Rhea organises Entrepreneur Country's bi-annual Forums, facilitating a full day of networking for 400 entrepreneurs and investors, as well as numerous workshops for the Entrepreneur Country community, incorporating partners and sponsors. More recently, Rhea organised Ariadne Capital's Follow the Entrepreneur Summit, a two day residential for 200 high net-worth individuals.
Rhea's expertise lies in both events management and recruitment and has worked for the likes of KPMG and Morgan Stanley. She also has a BA Degree in Business and Management from Hertfordshire University.
Tom Peterson
Marketing Manager - Entrepreneur Country
Tom joined Entrepreneur Country in 2010 as Marketing Manager having previously worked in online for Incisive Media. His responsibilities have included growing the Entrepreneur Country community through social media & marketing campaigns while developing new digital offerings for the Entrepreneur Country brand.
Tom became involved in online marketing after completing his BA Marketing and Advertising degree from Hull University. A keen traveller,
Tom has lived across Eastern Europe, Southern Africa and briefly in America.
Travis Baxter
Operating Partner
Travis Baxter has thirty years experience of consumer media with specific expertise in content development, consumer change and business strategy. He spent the first 5 years of his career at the BBC and since then his experience includes working at divisional MD level for RTL group and the FTSE 100 company EMAP and working as an operating partner with Advent International. Travis is currently media advisor to the Turkish private equity firm Actera, a non-executive director of South West Screen and of Slicethepie, and is Content and External Affairs Director of Bauer Media in the UK. He also sits on the board of a number of UK radio trade bodies.


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Tekken 3
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Game Detail
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- Release: Apr 29, 1998 »
- Also on: ARC
Titles rated T (Teen) have content that may be suitable for ages 13 and older.


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Stark News Details
Gasland to be Screened at Kent State Stark
Posted Apr. 6, 2011
The award-winning documentary Gasland will be screened at Kent State University at Stark on Thursday, April 21 at 7 p.m. in the Main Hall Auditorium, located at 6000 Frank Avenue NW in Jackson Township. This event is free and open to the public.
The largest domestic natural gas drilling boom in history has swept across the United States. The Halliburton-developed drilling technology of "fracking" or hydraulic fracturing has unlocked a "Saudia Arabia of natural gas" just beneath us. But is fracking safe? When filmmaker Josh Fox is asked to lease his land for drilling, he embarks on a cross-country odyssey uncovering a trail of secrets, lies and contamination. A recently drilled nearby Pennsylvania town reports that residents are able to light their drinking water on fire. This is just one of the many absurd and astonishing revelations of Gasland. Part verite travelogue, part expose, part mystery, part bluegrass banjo meltdown, part showdown. The film has won numerous awards, including the Special Jury Prize for a Documentary at the 2010 Sundance Film Festival.
For additional information about the Gasland screening, contact Derek Wholihan at email@example.com. To learn more about the film, visit www.gaslandthemovie.com.


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Impresa en: diciembre del 2010 Autor: Array
Describe a study of newly arrested youths where researchers found correlations between the youths' prevalence of these sexually transmitted diseases and their cocaine and marijuana use.
Summarizes a study to determine whether a stress-related biological marker in saliva can predict how long a drug user will remain in treatment.
Reports on NIDA's research priorities—drug abuse and addiction prevention, treatment, and HIV/AIDS—which will remain consistent during these challenging budget times.


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Crunchy roast potatoes
Here it is... the big secret to making crispy roast potatoes! Boiling them first will also cut down on the amount of time they spend in the oven- freeing up space for your ham, turkey and other vegetables! Serves 4 - 6
Cooking Time:30 minutes
Level of Difficulty:Easy
- 675 g/11 lb potatoes peeled and cut into large even-sized chunks
- Vegetable oil
- Preheat the oven to 220°C/425°F/Gas 7. (If you are cooking your roast potatoes in the same oven as the turkey at 180°C make sure to add on some extra oven time to ensure that the potatoes are cooked
- Place the potatoes in a pan of cold salted water and bring to the boil. Reduce the heat, cover and bring to the boil then simmer for 10 minutes until the outsides have just softened
- Meanwhile, preheat a roasting tin with 2 tbs of oil for a few minutes in the oven
- Put the lid firmly back on the potatoes saucepan and shake, so that the poatoes move around and their outsides get a bit bashed
- Lift the roasting tin out of the oven and set on top of the cooker. Carefully add the bashed potatoes one by one to the hot oil (take care!) and brush potatoes with oil using a pastry brush or spoon over the surface
- Place the roasting tin with the potatoes back in the oven and cook for 15 minutes. Using a large wooden spoon, turn potatoes over to coat them again
- Place back in the oven for a further 15 minutes
- Test with a skewer to make sure the potatoes are cooked- they should be crunchy on the outside and soft on the inside.


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2013 Criterium attire |
Anyways...team camp isn't quite here but the raw materials are starting to pile into the service course in Winstom-Salem, North Carolina. I myself will be heading down to live in Athens, Georgia on the first of February and am super amped to get my wintered hands on these fresh items. I'm also super stoked to get this jersey out and about! A big thank you to Champion System clothing and Winsor Creative for cooking up this stellar design.
Reynolds Wheels..before... |
Reynolds Wheels...after...wow...pretty! |
Bar tape! |
Keen Shoes! |
Tifosi Sunglasses |


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Set to Grill Sarah Palin
For the third time this season, we're told Tina Fey will be back, hysterically not answering questions as Palin. You betcha! No word yet on who has been tapped to play Biden.
Thanks to Latifah -- and since "SNL" has no female African-American cast member -- the world is saved from seeing Amy Poehler in black face.


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Browns notes: Richardson says Browns 'let the whole city of Cleveland down' (with video)
All signs seemed to point toward the Browns closing the home portion of the 2012 season Sunday on an upbeat note.
They had won three straight games to improve to 5-8 and placed themselves on the fringe of the AFC wild-card playoff chase.
The defense seemed to be coming into its own, limiting opponents to an average of 12.7 points in the three victories.
Rookie quarterback Brandon Weeden and the offense weren't setting the world ablaze, but they averaged 23.3 points in the three victories while making plays when plays needed to be made.
So much for the signs.
In a 38-21 loss to the Redskins, the Browns got beat every which way but loose.
In the quiet Browns locker room, many of the players admitted to being surprised at the poor performance by the home team.
Rookie running back Trent Richardson scored two rushing touchdowns but gained only 28 yards on 11 carries.
"This was very surprising," Richardson said.
"The way we game-planned for the entire week, I thought there'd be a better outcome. But you have games when outrageous stuff happens," Richardson added. Continued...
"We let ourselves down. We let the whole city of Cleveland down, especially the season-ticket holders. I feel bad for them. As a man, I want to apologize. It was a big ball game. We didn't do what we were supposed to do."
The team's elder statesman, kicker Phil Dawson, said the team's mettle will be tested in upcoming road games against Denver and Pittsburgh.
"Now, we have to deal with the reality the playoffs are no longer a possibility," Dawson said. "I have no doubt these guys will show up for practice this week and work hard. They've been doing that for five months."
Tips of the hat
Most of the Browns' players who spoke with reporters after the game had words of high praise for Washington quarterback Kirk Cousins.
The rookie from Michigan State did a great job filling in for the injured Robert Griffin III, completing 26 of 37 attempts for 329 yards and two touchdowns.
Cousins' coach, Mike Shanahan, offered a matter-of-fact assessment of Cousins' contributions to a clutch road victory for the playoff-contending Redskins.
"We're always going to put together a game plan that we think fits the players that are playing,'' Shanahan said. "So, whoever it is — a tight end, receiver or quarterback — we are going to make adjustments. I thought (Cousins) did a great job.'' Continued...
Much higher priority
At the end of his postgame session with reporters, Browns coach Pat Shurmur shared some heartfelt thoughts about last week's tragic school shooting in Newtown, Conn.
"It's very disturbing stuff,' Shurmur said. "I know our players have talked about it a bunch. We've talked about it together.
"If we can learn anything from it, let's not leave anything unsaid," Shurmur added. "If you've got an opportunity to hug your child, let's not let anything go because tomorrow and anything happening tomorrow is not guaranteed.''
Injury report
Two of the Browns' defensive starters, safety T.J. Ward (left knee) and end Frostee Rucker (groin), were forced to the sideline and did not return.
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ROCKINGHAM — Before the season started, Richmond Senior girls basketball coach Victoria DeFrate kept stressing defense, defense, defense.
Her team paid attention.
Richmond limited Douglas Byrd to one field goal in the first and third quarters en route to a 62-25 nonconference victory Friday night at Raider Gymnasium.
"I was excited to see improvement from Tuesday," DeFrate said. "The girls are starting to see that our defense creates offense. Your shot may be off, but you can rely on your defense."
The Raiders (2-0) used their defense to hold the Eagles without a basket until Audraya Doyle scored with 6.4 seconds remaining in the first period. The long drought allowed Richmond to build a 15-5 advantage.
After extending the lead to 35-13 at halftime, the Raiders limited the visitors to two points in the third quarter — a putback by Doyle at the 3:07 mark.
"After Tuesday's game I asked the girls what they thought they needed to improve on," DeFrate said. "I could see the enthusiasm in the next practices. They are beginning to see the things they have to do that us coaches have been harping on in practice."
Just like its season-opening win at South Robeson, only one player scored in double figures for Richmond — Shaquail Wall with 16. Cherie Ellerbe added nine points in the victory.
Doyle led the way for Douglas Byrd with eight.
The Raiders will return to action 6 p.m. Tuesday when they travel to Cheraw, S.C.
— Sports editor Shawn Stinson can be reached at 910-997-3111, ext. 14, or by email at email@example.com.


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Product Description
Nothing beats the soft comfort of this curve-hugging women's NBA custom long-sleeve t-shirt. It's fitted to accentuate your feminine shape and features your choice of graphics heat sealed on the chest, back, and sleeves.
Country Of Origin: Honduras
In stock, leaves warehouse in 2 - 3 full bus. days. - (Details)
Standard Ground Service Available - (Details)
Note: Shipping restrictions may apply.
Remember, no sales tax except in FL, IL, KY, NY, OH, PA and TX.
- Grey: 90% cotton, 10% polyester
- Other Colors: 100% cotton jersey
- Fitted long-sleeve shirt
- This tee is designed with a figure-forming fit. Purchasing a larger size is advised
- Crew neckline
- Front: Choose large full-chest logo or small left-chest logo
- Back: Choose large logo, player name and number, or personalized name and number
- Sleeves (either or both): Choose logo or number, or leave blank. Number can only be selected if also on the back and must match the back number. If no back number is chosen, the only sleeve option is logo
- All graphics are heat sealed
- Decorated in the team colors
- Machine wash and dry
- Officially licensed
- Made in Honduras
Reviewed by 1 customer
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Comments about NBA Custom Denver Nuggets Womens Custom Long Sleeve T-Shirt:
I ordered a custom shirt and the logo was noticably off center and crooked. Customer Service was very sympathetic and placed a new order. Promised I'd have it in a week and it's been almost two weeks and still don't have it. Wanted it for a specific event last week!! :(
Displaying review 1


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Welcome, we're here to help you!
Web Designs Boise offers top-quality professional Web Design and Development services.
We specialize in custom content creation and programming, and we are experts in WordPress, Joomla, Zen Cart and Magento installations and customizations.
Further, we can help you with your web hosting needs too with our own servers and 24/7 technical support.
Call us today at (208) 424-0125 for a free project price estimate.


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Add feed or gadget | This lively pet hamster will keep you company throughout the day. Watch him run on his wheel, drink water, and eat the food you feed him by clicking your mouse. You can customize the color by cli... http://hosting.gmodules.com/ Randomly presents quotes from the most famous and important people of the world! http://quotes4all.net/ Collection of optical illusions - an easy way to trick the human eye. http://brainden.com/ Reviews and previews of movies from print, online, and broadcast critics. http://i.rottentomatoes.com/ Some hungry pet turtles to play around with on your homepage. Feed the turtles by clicking the water. Drag them around and watch them swim after the food. Add more turtles and customize the colo... http://hosting.gmodules.com/ Optical Illusion of The Day gadget displays the newest illusion in your iGoogle homepage each day. http://www.moillusions.com/ | Search for gadgetsLearn more about iGoogle |
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Seems like everyone who's anyone is hitting the Toronto International Film Festival
Ministry of GossipWith dozens of premieres, screenings and Q&A sessions, plus parties to match, is there really a good reason for an actor not to attend the 2010 Toronto International Film Festival? Makes sense that Hollywood -- and by that we mean East Coast, West Coast... -
Stars line up for Stephen Elliott's film
Jacket CopyAuthor Stephen Elliott will direct his first film; stars Heather Graham, James Franco, Lili Taylor and Dev Patel are in talks to appear.... -
Aniston, 'Airbender' dominate Razzies
Frankly My Dear - Orlando SentinelThe Golden Raspberry Foundation has pursed its lips and let the spritz hit the fan, unleashing its annual takedown of the worst, the very worst in movies. Jennifer Aniston is about to have her Sandra Bullock moment. With no Oscar to ease the pain. And... -
See which locally shot movie is top nominee -- for worst awards
TV WatchersThe movie "The Last Airbender," which has scenes filmed in Berks and Carbon counties, looks like it's about to sweep the awards. Unfortunately, the awards for which it's nominated are the 31st annual Golden Raspberry Awards —... -
'The Last Airbender'
Zap2ItThe animated series that was a popular attraction on Nickelodeon gets elaborate live-action treatment from director M. Night Shyamalan ("The Sixth Sense"), but some fans of the franchise still might appreciate the original version more. Noah Ringer plays...Tags: The Last Airbender (movie), Entertainment, Documentary (genre), Blu-ray Discs, Slumdog Millionaire (movie)
Movie Review: The Last Airbender
M. Night Shyamalan peers over the edge and into the abyss with The Last Airbender. Yes, that's water ahead of him, water all around him. And it's swirling. This colossal folly, the fiasco of the summer of 2010 -- gives us all a ringside seat at the...Tags: Star Wars (movie), Entertainment, Slumdog Millionaire (movie), PG Rated Movies, Noah Ringer
Working the refs: protestors pepper critics re: Last Airbender
Frankly My Dear - Orlando SentinelI've been hit with a few pieces of "grass roots" protest or astro-turf protest in the past couple of hours. They're from folks supposedly representing groups that are up in arms over Hollywood's casting of actors not of the specific race of... -
Avatar: The Last Airbender, MORE racial blowback
Frankly My Dear - Orlando SentinelOur spirited discussion of the racial implications of how M. Night Shyamalan's film of the popular TV 'toon Avatar: The Last Airbender, continues with a few words from Guy Aoki, who runs something called Media Action Network for Asian Americans.... -
Monday's TV Talk Shows
Show TrackerThe Early Show Bret Michaels; Dylan Lauren, daughter of Ralph Lauren. (N) 7 a.m. KCBS Today Taylor Lautner; Laura Vanderkam. (N) 7 a.m. KNBC KTLA Morning News Dev Patel; David Alan Grier; Harry Hamlin. (N) 7 a.m. KTLA Good Morning...... -
Last Airbender–the racial Catch 22
Frankly My Dear - Orlando SentinelAs I watched M. Night Shyamalan's film of The Last Airbender last night, one thing missing from this large and fractious racial debate over the "racebending" casting is mention of the trap that any filmmaker finds him or herself in with a... -
Thursday's TV Talk Shows
Show TrackerClick here to download TV listings for the week of June 27 - July 3 in PDF format This week's TV Movies Weekly TV Listings can also be downloaded here The Early Show Camping inexpensively. (N) 7 a.m. KCBS Today...... -
Will 'The Last Airbender' rank first with the Razzies?
Gold DerbyWhile "The Last Airbender" did well at the box office, taking in almost $70 million over the July 4 weekend, it failed to impress the critics, managing an aggregate rating of just 20 at Meta Critic. That poor score is the fourth lowest of the year and...
Sep 14, 2010
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| Los Angeles Times
Original site for Dev Patel topic gallery.


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Below Zero at Dead by Dawn
Feb 23, 2012
Below Zero at Dead by Dawn
Below Zero has been accepted to 14 international film festivals and counting! Catch it at Dead by Dawn in Edinburgh, March 31, Boston or Santa Cruz in April and May respectively.
Scored by Core composer Jeff Tymoschuk, the movie has been described as "A well-crafted film", "Multilayered and open to interpretation" and "Blurring the line between a horror film and a very dark psychological thriller."