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Responsibility for the maintenance of a bus shelter can fall with either Cheshire East Council or the Parish / Town Council, depending on which authority provides and owns it.
Clear Channel (Adshel) has a contract with the council to provide advertising shelters which are installed and maintained at no cost to the Council.
For further information or to report damaged infrastructure contact the bus stop infrastructure team on
Requests for new stops or for existing stops to be upgraded can be made direct to Cheshire East Council using the contact details above.
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Use the contact details above to request accessibility improvements to a bus stop.
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Light rainfall and cloudy skies are on the India Meteorological Department (IMD) forecast for Delhi-NCR on Friday.
This monsoon season so far, from June 1 to September 16, a total of 1,170.7 mm of rainfall was recorded. This is the highest for the entire monsoon season in the past 57 years, IMD officials said. The figure for this monsoon season stands close behind the highest amount recorded for the season – 1190.9 mm in 1964.
Gloomy skies and intermittent rainfall over the past few days have ensured a dip in temperature. The maximum temperature on Friday is likely to be 30 degree Celsius, while the minimum is likely to stand at 24 degree Celsius. The maximum temperature on Thursday was 28 degree Celsius, around 6 degrees below normal.
Relative humidity at 8.30 am Friday was 86 per cent. The possibility of rainfall remains on the IMD forecast for the next six days.
Between September 18 and September 21, the city is likely to witness light rainfall, with the possibility of thundershowers on September 22 and September 23, going by the IMD's seven-day forecast.
From September 1 to September 16, the Safdarjung observatory of the IMD recorded a total of 404.1 mm of rainfall.
The IMD has said conditions will not be favourable for the commencement of withdrawal of the monsoon from northwest India till September 29. A cyclonic circulation is likely to form over the Bay of Bengal next week. The formation of cyclonic circulations over the Bay of Bengal has kept monsoon conditions active over parts of northwest India.
Most parts of Delhi recorded light rainfall on Thursday as well. Between 8.30 am on Thursday and 8.30 am on Friday, the Safdarjung observatory, which provides representative figures for the national capital, recorded 12.9 mm of rainfall, while the station at Palam logged 9.1 mm, Lodi Road saw 13.4 mm and the Ridge recorded the highest amount of 19.6 mm.
According to an update from Thursday, a well-marked low pressure area lies over parts of north Madhya Pradesh, bringing normal rainfall activity to northwest India.

subset: commoncrawl


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Freshman snags softball squad's starting second-base spot
Freshman Allie Colleran has filled the hole at second base for the softball squad.
Freshman Allie Colleran has filled the hole at second base for the softball squad.
The Ithacan Sports staff recaps the past week in Bombers sports.
The softball team recorded its ninth win in 11 games.
The softball team's shortest player has left one of the biggest impacts so far this season.
Though it is officially the spring season, Kostrinsky Field still lies empty, because the Ithaca area winter conditions have done some damage. The weather has left the field with frozen dirt, raw grass and a few scattered patches of snow. Until the weather becomes warmer and the remaining snow melts off the field, the Bombers…
The softball team entered its five-series road trip with an overall record of 9-10. But with a rejuvenated offense and solid pitching, they have surged above the .500 mark and currently hold a mark of 18-11 overall following yesterday's doubleheader sweep against King's College.
While the softball team's power has been its driving force in Empire 8 Conference games, its ability to put runners on base has helped it seal eight of its 16 wins this season.


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Mckinney Thomassen posted an update 9 months, 3 weeks ago
Dump Trucks are the most important part of the commercial trucks on the market industries, societies and in addition with the industries. These trucks are having their special coverage and performance base to make the trucks on the market business more pleasing and dedicated.
The Definition
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The Structure
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The kinds
There are several and specified forms of trucks of Dump trucks. These kinds are separated in line with the bed types and also the kind of dumping. Also these trucks varies with the help of material containing elements and themes. Only at the web page we've got defined the different sorts of Dump trucks.
* Standard Dump trucks
A regular dump truck is really a chassis that produces the dump body together with the mounted frame that is along with the hydraulic ram used for raising the bed along with the front body with the dumper. The rear of the trucks is hinged within the side with the truck. Also the tailgate in the truck is configured because of swings and hinges which is attached with the top Lift Tailgate. These trucks can be found in the type of six wheeler and ten wheeler dump trucks version. The greatest Dump truck is named the "centipede" which is having seven axles and it is able to hauling 32 tonnes gross weight which is well liked in European and American countries.
* Articulated Dump trucks
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So, these are some of the leading varieties of trucks and the trucks are therefore very popular and demanding inside the truck lovers. Possess the best section of truck models and have the most accepted forms of Dump trucks.
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Kell Brook Totally Frustrated Making Amir Khan Fight Happen
Kell Brook Totally Frustrated Making Amir Khan Fight Happen
Domestic rivals Amir Khan and Kell Brook seemed to be on a collision course some years back but now it's all about the wait for the later, who after being brought up to Khan by Eddie Hearn in an Instagram video chat, ended with question of his ability to still make the 147 pound limit.
The former IBF welterweight champion Brook expressed his frustration as part of his own live Instagram video chat with Eddie Hearn, claiming to have given up on Khan because efforts to make the fight happen has been fruitful.
"I've given that angle up because there's no more I can [do], you know, because it's gone past the line now, for me," Brook said to his promoter. "I've done everything that's been asked of me to make it happen. It's still gone nowhere."
Sheffield's Brook is presently interested in facing someone entirely different, the author of Khan's most recent defeat Terence Crawford and he seemed more optimistic the fight was going to happen than an unfruitful wait for Khan.
On Your Favorites Sports
The unbeaten WBO Welterweight champion Terence Crawford (36-0, 27 KOs) has picked Brook as one of the potential opponents for his title defence, ones the Corona Virus Pandemic is over. However, despite the his optimism in the potential fight against Crawford, he didn't entirely rule out the fight with Khan.
"With me and Khan, you know, we can talk," Brook said. "When the money's right, we can make the fight happen."
Over the years, Khan has made clear, he doesn't like the idea of fighting Khan above the Welterweight limit as Brook hasn't made the welterweight limit since losing his IBF title to Welterweight champion Errol Spence via an eleventh round knockout back in 2017.
Khan has fought only twice above the Welterweight limit. First, in his knockout loss against boxing Star Canelo Alvarez back in 2016. A WBC championship fight that was contracted at a middleweight limit of 155 pounds.
Amir Khan is subsequent fight against Lo Greco was fought at a 150 pound limit, a fight he won with a first round knockout back in 2018.


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Saturday 21st September 2013
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The 2013 England Athletics ultra distance championships were incorporated into the Self Transcendence 24 hour race held at Tooting Bec track in South London at the weekend (Sept 21st/22nd)
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Study on the value of antibiotic‑resistant gene detection in Helicobacter pylori in China
- Authors:
Affiliations: Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China
- Published online on: January 18, 2022 https://doi.org/10.3892/etm.2022.11153
- Article Number: 228
Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
This article is mentioned in:
Helicobacter pylori (H. pylori) is a spiral-shaped, microaerophilic and Gram-negative bacillus that was isolated by Marshall and Warrenin 1983(1). H. pylori infection has been considered as an independent risk factor for gastric cancer for 27 years (2). Both laboratory and clinical studies have confirmed that H. pylori is a key factor in the occurrence of gastric cancer. The Kyoto consensus published in 2015 reported that H. pylori infection is an infectious disease. The earlier H. pylori is eradicated, the lower the incidence of gastric cancer will be. The eradication program should contain the most effective antibiotics available (3). It has been reported that eradication is more successful when antibiotics are selected based on individual susceptibility, community antibiotic sensitivity testing or antibiotic use and clinical prognosis data (3). However, the increased antibiotic resistance rates have become a severe challenge for the clinical eradication of H. pylori. The resistance rate to tetracycline (TET), amoxicillin (AMX) and furazolidone (FUR) in the majority of countries and regions in the world is relatively low (<10%) (4-10). However, it has been reported that the abuse of antibiotics in Iran has led to a high antibiotic resistance rate of H. pylori to TET (38.5%), AMX (27.1%) and FUR (23.9%) (11). As of June 2017, in all World Health Organization regions, the primary and secondary resistance rates of clarithromycin (CLA), metronidazole (MNZ) and levofloxacin (LEFX) were ≥15%, except for the primary and secondary resistance rates of CLA in America (10%; 95% CI, 4-16%) and Southeast Asia (10%; 95% CI, 5-16%) and LEFX in Europe (11%; 95% CI, 9-13%), while the global double resistance rate of CLA and MNZ was 19% (12). It is generally accepted that the main detection method of the antibiotic resistance of H. pylori is a drug sensitivity test based on the in vitro culture of H. pylori (13). Since H. pylori is a microaerophilic bacterium, its in vitro culture is challenging and time-consuming. In addition, numerous hospitals do not dispose the facilities and equipment to carry out this test. A previous study has indicated that there are several resistance mechanisms of H. pylori to antibiotics, including enhanced oxidative free radical scavenging activity, increased DNA repair enzyme activity, drug efflux pump expression and activation, as well as resistant gene expression, which is the focus of the present study (8). The Vaculationg cytotoxin gene A(VacA) gene exists in almost all H. pylori strains. However, only ~50% of the strains can express VacA, an important virulence factor in H. pylori production that causes vacuolation (13). The VacA gene is divided into middle region types m1 and m2, intermediate region i1, i2 and i3 and signal sequence types s1a, s1b, s1c and s2(14). The association between VacA subtypes and H. pylori resistance remains controversial (15,16). The detection technology of drug-resistant genes is simpler and faster than the drug sensitivity in vitro culture-based test, and it can detect the H. pylori genotype at the same time (17). Therefore, the present study explored the value of antibiotic-resistant gene detection in H. pylori and the association between genotype and antibiotic resistance.
Patients and methods
Patients with H. pylori infection who were subjected to gastroscopy were enrolled between December 2017 and January 2020 in The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang, China). A total of 133 H. pylori-positive patients were enrolled in the present study, with a mean age of 45.1 years (age range, 18-71 years). The inclusion criteria were as follows: i) Age, 18-70 years old; ii) pathological findings of H. pylori infection; and iii) positive in [13C]-urea and [14C]-urea breath tests. The exclusion criteria were as follows: i) Gastroscopy contraindications; ii) diagnosis of severe cardiovascular diseases, immune disorders, poorly controlled diabetes, hypertension or recently planned subtotal gastrectomy; iii) inability to obtain biopsies due to poor coagulation function or anticoagulant drugs; iv) inability to complete gastroscopy due to lack of cooperation; and v) refusal to participate in a clinical trial.
The present study was approved by the Ethics Committee of The First Affiliated Hospital of Zhejiang Traditional Chinese Medical University (approval no. 2017-KL-054-02; Hangzhou, China). All patients provided written informed consent and general information, such as sex and age. Gastric mucosa samples were obtained from all patients (three samples per patient; each sample was 0.4-0.6 mm2). One sample was sent for routine pathological examination, one was cultured in vitro and subjected to a drug sensitivity test (agar plate dilution method), and one was sent for antibiotic resistance gene detection (PCR reverse dot blot; a chip with mutation sites presented in Table I). Finally, the sensitivity of H. pylori detection was compared between the two methods. The mutation sites tested in the present study were as follows: C556G, A562T and A562G in PBP1 for AMX; T87A, A91G, G91T and G91A in gyrA for LEFX; 926-928 (AGA to TTC) and 926-927 (AG to GT) in 16S rRNA for TET; A2142G and A2143G in 23S rRNA for CLA; and G616A in rdxA for MNZ (Table I). The mutation rate of the related gene sites was calculated in different antibiotic-resistant strains and the antibiotic resistance rate was calculated in gene mutant strains. Finally, the drug resistance rate was compared among different VacA genotypes.
H. pylori culture and drug sensitivity test H. pylori culture
Gastric mucosa tissue immersed in Bacterial Preservation Fluid (Hangzhou ZhiYuan Medical Laboratory) was aspirated using a sterile pipette. A total of 0.5 ml RIPA Lysis Buffer (Hangzhou ZhiYuan Medical Laboratory) was to form tissue homogenate. The tissue homogenate was then added to the culture medium of Columbia blood agar (500 g; Thermo Fisher Scientific Inc.). The medium was incubated in a microaerobic environment (5% O2; 10% CO2; 85% N2; 37˚C) for 2-3 days. The suspected colonies were selected for hematoxylin-eosin staining (6.0% hematoxylin for 5 min and 0.5% eosin 2 min at room temperature) and were subjected to three enzyme identification tests [urease (Suzhou Haibo Biotechnology Co., Ltd., 37˚C, 180 min), oxidase (Beijing Luqiao Technology Co., Ltd., room temperature 5 min) and catalase (Suzhou Haibo Biotechnology Co., Ltd. room temperature 5 min; data not shown]. If the cultured bacteria were identified as H. pylori, the result was positive; otherwise, it was negative.
Drug sensitivity test of H. pylori
The H. pylori colonies were selected in sterile normal saline, shaken and mixed well. Next, the bacterial suspension containing 0.5 MCF (150 million bacteria/milliliter) was prepared. A total of 3 µl bacterial suspension was collected using a pipette gun and added to the blood agar medium, with and without the following antibiotics: CLA (100 mg x 10 tablets; National Medical Products Administration), LEFX (100 mg x 10 capsules; China Academy of Food and Drug Control), MNZ (100 mg x 10 tablets; China Academy of Food and Drug control), AMX (0.25 g x 30 capsules; Dr. Ehrenstorfer GmbH). Three gas incubators were purchased from Thermo Fisher Scientific, Inc. (cat. no. 3427). The antibiotic concentration was the drug-sensitive minimum inhibitory concentration of the antibiotic: 1 clarithromycin, 2 levofloxacin, 2 amoxicillin, 2 furazolidone, 2 tetracycline and 8 µg/ml metronidazole. The medium was incubated in a microaerobic environment (5% O2; 10% CO2; 85% N2; 37˚C) for 2 days. The growth state of the strain was first observed in the culture medium without antibiotics. If it grew normally, the drug sensitivity test result could be interpreted; otherwise, the test needed to be repeated. Next, the colony growth state was observed in the culture medium containing antibiotics. If the colony growth was inhibited, H. pylori was considered to be sensitive to the antibiotic; otherwise, H. pylori was considered to be resistant to the antibiotic.
PCR reverse dot blot Preservation of samples and sample processing
Gastric mucosa samples were preserved at -80˚C. For genotyping and drug resistance gene mutation testing of H. pylori, a nucleic acid extraction reagent was used [Nucleic acid extraction kit, Yaneng Biosciences (Shenzhen) Co., Ltd., cat. no. Yueshen Xiebei no. 20150098; model no. pathogen DNA (centrifugal column type); specification, 25 copies/box]. The concentration and purity of the template DNA prior to PCR was determined using a nucleic acid quantitative analyzer (Multi-mode Readers BioTek).
Preparation of amplification reaction solution
The sequences of the gene primers used are listed in Table II. The number of reaction solutions required for each test was n+2, where n=number of samples to be tested, and 2 equals one H. pylori positive quality control sample and one H. pylori negative quality control material. Reaction solutions I, II and III were removed from the reagent box, melted and mixed. The amplification reaction solution was prepared according to the manufacturer's instructions. After the mixture was prepared, it was evenly mixed and centrifuged at 447.2 x g for 10 sec at 4˚C.
Sample addition
The sample DNA was tested (three times per sample; 4 µl each) and added into three different amplification reaction solutions. Quality control samples (DNA of known dose) were also included (three times per sample; 4 µl each) and added into three different amplification reaction solutions for PCR amplification.
PCR amplification
PCR was performed using the H. pylori Genotypes and drug resistance mutations detecting kit (PCR-reverse dot blot; Hangzhou Qianji Biosciences Co., Ltd. China). The PCR parameters are presented in Table III. The temperature rise and fall rate was 3.0˚C/sec.
Film strips marked with the sample number were placed in a plastic centrifuge tube (15 ml), and 7 ml liquid A was added. Next, three PCR products corresponding to the sample number were added, and the tube cover was tightened. Next, the centrifuge tube was heated in a boiling water bath for 10 min. It was then removed, the cover was tightened, and the tube was placed into the hybridization instrument (Combi-H12 FINEPCR) at 48˚C for 1.5 h. A total of 45 ml liquid B was added to a 50 ml plastic centrifuge tube, which was then preheated at 48˚C in the hybridization instrument.
Membrane washing
The membrane strips were removed and transferred to a 50 ml tube containing preheated liquid B, followed by gentle washing at 48˚C for 15 min.
Color rendering
The formula of the incubation solution was as follows: Solution A:Peroxidase=2,000:1. The membrane was washed twice with liquid A at room temperature for 5 min each time. It was then washed with liquid C at room temperature for 1 min, and the color developing solution was prepared. The membrane strip was then immersed in the color developing solution at room temperature for 10 min and rinsed with pure water twice to observe the results. Subsequently, mutation points turned blue (Fig. 1).
Figure 1
Membrane strips. (A) S1/M2/UreA/CagA; sensitive to all the antibiocs tested. (B) S1/M2/UreA/CagA; resistant to clarithromycin (23S rRNA A2143G) and metronidazole (rdxA G616A). (C) Helicobacter pylori negative.
Statistical analysis
Fisher's exact test was used to compare two groups of classification data. The χ2 test was used for comparison of multiple sample rates. SPSS v17.0 (SPSS, Inc.) statistical software package was used for statistical analysis. P<0.05 was considered to indicate a statistically significant difference.
General patient information
The male:female ratio of the enrolled patients was 1.17:1 (P>0.05). A total of 111 strains were successfully cultured in vitro, 109 strains were identified by pathologists (Fig. 2), while 108 strains were detected using PCR reverse dot blot. No significant difference in the sensitivity of H. pylori detection was identified between the three methods (agar plate dilution method, 83.5%; PCR, 81.2%; pathology, 82.0%; P=0.89). A total of 96 antibiotic-resistant strains were confirmed by both the agar plate dilution method and PCR reverse dot blot. According to genotyping, all strains were VacA-positive and type s1, including 44 cases that were type s1m1 and 69 that were type s1m2. The agar plate dilution method revealed that the resistance rate of MNZ was 90.1% (100/111), followed by 35.1% (39/111) for LEFX and 32.4% (36/111) for CLA. No strains were resistant to AMX, FUR and TET. A total of 34 strains were resistant to both CLA and MNZ, 37 strains were resistant to both LEFX and MNZ, and 23 strains were resistant to CLA, LEFX and MNZ.
Figure 2
Pathological results. (A) Helicobacter pylori negative (magnification, x200). (Β) Helicobacter pylori positive (magnification, x100).
Association analysis between mutations of different gene loci and drug resistance
The results of the agar plate dilution method and PCR reverse dot blot were not entirely consistent; 32.1% of strains that, according to the agar plate dilution method, were MNZ-resistant exhibited an rdxA G616A mutation. In addition, numerous LEFX-related resistance gene sites were identified in gyrA. Among them, the T87A mutation accounted for 32.3%, the G91A mutation for 22.6%, the A91G mutation rate for 12.9% and the G91T mutation for 6.5%. The main mutation in CLA-resistant strains was 23S rRNA A2143G, accounting for 81.8%, with the 23S rRNA A2142G mutation accounting for 0.0%. Neither AMX-resistant nor TET-resistant strains were identified in vitro (Table IV).
Table IV
Proportion of gene mutations in drug-resistant heliobacter pylori strains shown by agar plate dilution.
Among all the gene sites studied, rdxA G616A had the highest accuracy in predicting H. pylori resistance (81.8%), followed by gyrA G91T (66.7%), gyrA G91A (54.5%), 23 S rRNA A2143G (49.5%), gyrA T87A (45.5%), gyrA A91G (33.3%) and 23S rRNA A2142G (0.0%), while no drug resistance was identified in the strains with AMX-(PBP1 C556G, PBP1 A562T and PBP1 A562G) and TET-resistance-related gene mutations [926-927 (AG to GT) and 926-928 (AGA-TTC) in 16S rRNA], according to the in vitro drug sensitivity test (Table V).
Among the genes, 81.8% of strains with a rdxA mutation were revealed to be resistant to MNZ, while 47.8% of strains with a gyrA mutation and 49.1% strains with a 23S rRNA mutation were indicated to be resistant to LEFX and CLA, respectively (Table VI).
Drug resistance analysis of different VacA genotypes
All 108 patients with positive H. pylori genes presented the VacA s1 type; there were no VacA s2 type patients. Out of those, 39 (36.1%) had VacA s1m1 strains and 69 (63.9%) VacA s1m2 strains. As indicated using Fisher's exact test and χ2 analysis, there was no significant association between genotypes and overall drug resistance rate, multidrug resistance rate, CLA, LEFX or MNZ resistance rate (P>0.05; Table VII, Table VIII, Table IX, Table X and Table XI).
There are several mechanisms of H. pylori antibiotic resistance, and they vary among different antibiotics (8). The mechanism underlying CLA resistance involves reverse binding to the domain V of the 23S rRNA gene in the ribosomal subunit 50S in bacteria, thereby interfering with protein synthesis and promoting the early release of peptide tRNA from the recipient site, thus preventing the synthesis of the H. pylori peptide chain extension protein (18). Therefore, a mutation in domain V of the 23S rRNA gene may decrease the affinity between the ribosome and drug, leading to the lack of binding between CLA and 50S ribosome subunit. If the mutation cannot affect protein synthesis, H. pylori resistance occurs. In addition, if the mutation cannot entirely block the affinity between ribosome and antibiotics, sensitivity to antibiotics can still occur in vitro. Among the point mutations, certain are clinically more important than others. Seo et al (19) reported that 19.5, 0.9, 0.2 and 0.7% patients presented A2143G, A2142G, A2142C and both A2143G and A2142G mutations, respectively. The CLA-resistant group was treated with proton-pump inhibitor (PPI) twice a day, AMX (1,000 mg) twice a day and MNZ (500 mg) three times a day for 7 days (PAM). Groups with other mutations were treated with PPI twice a day, AMX (1,000 mg) twice a day and CLA (500 mg) twice a day for 7 days (PAC). As revealed by ITT (Intention-To-Treat (ITT) and PP(Per-Protocol)analysis, there was a significant difference between the two groups [PAM vs. PAC, 55.4 vs. 74.3%, P=0.001 (ITT); 66.2 vs. 88.4%, P=0.0001) (PP)] (16). Similarly, Ong et al (20) treated patients infected with H. pylori with no CLA mutation points in 23S rRNA with concomitant (1,000 mg AMX; 500 mg CLA; 500 mg MNZ and 30 mg lansoprazole twice a day for 14 days) or tailored (1,000 mg AMX; 500 mg CLA and 30 mg lansoprazole twice a day for 14 days) therapy. There was no significant difference in the eradication rates between the groups in both point mutation-negative subjects (91.7 vs. 87.3%; P=0.154). These studies demonstrated that the detection of CLA mutation points in 23S rRNA could be efficient for screening of CLA resistant strains in regions where the CLA resistance rate is >15%. In the present study, the CLA resistance rate was as high as 32.4%, and the mutation rate of A2143G and A2142G in 23S rRNA was 81.8 and 0% (Table IV), respectively. The sensitivity of A2142G and A2143G mutation in 23S rRNA was 81.8%, rendering them ideal mutations for the detection of CLA resistance in H. pylori in China. Adding bismuth to standard triple therapy with CLA may eradicate 77.6% resistant strains with A2143G or A2142G mutation (21).
LEFX interferes with bacterial DNA synthesis by inhibiting DNA gyrase and topoisomerase IV (8). The gyrA gene encodes DNA gyrase, so its mutations can lead to the resistance of H. pylori to LEFX (22). Liou et al (23) used a modified sequential therapy containing LEFX and high-dose esomeprazole (40 mg esomeprazole and 1,000 mg AMX for the first 5 days, followed by 40 mg esomeprazole, 250 mg LEFX and 500 mg MNZ for another 5 days; all administered twice daily) in patients who did not respond to first-line treatment. The eradication rates were 84.6% (11/13) and 95.1% (58/61) in patients with and without a gyrA mutation, respectively (P=0.210), which indicated little value of gyrA mutation testing. However, Papastergiou et al (24) used a 7-day, genotypic resistance-guided triple H. pylori eradication therapy comprising esomeprazole, AMX and either CLA (wild-type 23S rRNA), LEFX (23S rRNA mutated/wild-type gyrA) or rifabutin (mutated 23S rRNA/gyrA). The mutation sites of CLA in Papastergiou et al (24) were A2146G, A2146C and A2147G in the 23S rRNA gene, while the mutation sites of LEFX were N87K, D91N, D91G and D91Y in the gyrA gene. For patients who received CLA-, LEFX- and rifabutin-containing triple therapy, the respective eradication rates were 24/27, 20/20 and 2/4 by ITT and 24/24, 19/19 and 2/3 by PP analysis. The overall eradication rates exhibited no significant difference between treatment-naïve and -experienced patients (ITT, 87.5 vs. 94.7%, P=0.64; PP, 96.4 vs. 100%, P=1.00). Therefore, the unsatisfactory results of the previous trial may be linked to the high resistance rate of MNZ. The present results indicated that 74.2% of LEFX-resistant strains had gene mutations. The mutation rates of T87A, G91A, A91G and G91T in gyrA were 32.3, 22.6, 12.9 and 6.5%, respectively. The resistance rate of loci 91 (36.1%) was similar to that in the study by Palmitessa et al (25). The coincidence rates of mutation sites, as determined by in vitro drug sensitivity test, were as follows: rdxA G616A (81.8%), gyrA G91T (66.7%), gyrA G91A (54.5%), 23 S rRNA A2143G (49.5%), gyrA T87A (45.5%), gyrA A91G (33.3%), and 23 S rRNA A2142G (0.0%). Since the resistance rate of gyrB is low (26), it was not detected in the present study, based on the economic benefit ratio. Meta analysis showed that second-line anti-H. pylori levofloxacin/amoxicillin-based triple (10-day regimen) therapy was significantly more effective than standard quadruple therapy [PPI+bismuth+two antibiotics; odds ratio, 5.05; 95% CI, 2.74-9.31; P<0.001; heterogeneity index (I2)=75%] (27). Simple molecular susceptibility testing for CLA and LEFX may achieve a high H. pylori eradication rate. If CLA or LEFX resistance does exist, administration of probiotic microorganisms along with triple therapy would be another treatment possibility (28).
In the present study, no case showed 926-928 and 926-927 loci mutations in 16S rRNA, a TET-related resistance gene and 556 and 562 loci mutations in an AMX-related drug-resistant gene, PBP1, occurred in 10 cases (C556G) and 3 case (A562T), respectively. However, TET- and AMX-resistant strains were not revealed to be among these mutant strains via the in vitro drug sensitivity test. At the same time, AMX-resistant strains detected in the in vitro drug sensitivity test identified no gene mutation. No FUR-resistant strains were cultured in the present study. In a review, Zamani et al (29) analyzed studies on the resistance of H. pylori to FUR published worldwide, suggesting that the resistance rate of H. pylori to FUR in most regions was <5%, which may be associated with A041G, A122G, C349A or G A78G, A112G, A335G, C156T and C165T mutations in the oorD gene and G353A, A356G, C357T, C347T, C347G and C346A mutations in the porD gene. The specificity and sensitivity of several mutation sites mentioned in the review by Zamani et al (29) requires further investigation, as they were not detected in the present study. Generally, the drug resistance rate of H. pylori to AMX, TET and FUR is low, and thus no drug resistance test is required prior to eradication if any of these three antibiotics are used (30) However, it should be noted that TET has been out of production for many years in China.
In a study by Lee et al (31), which enrolled 53 H. pylori-positive patients treated with MNZ-containing sequential or quadruple therapy between 2011 and 2015, rdxA and frxA mutations in patients with H. pylori were analyzed via DNA sequencing. The results indicated that rdxA mutations served a critical role in MNZ resistance and the outcomes of eradication therapy. Apart from rdxA, frxA and frxB are among the most frequently studied mutations; however, previous studies have suggested that mutations in these two genes have a synergistic effect with rdxA mutations in causing MNZ resistance, but cannot cause it alone (32-34). In theory, if the mutation rate of rdxA in resistant strains is high enough, tailored therapy could achieve a high eradication rate of H. pylori, according to the rdxA gene mutation detection results. However, only 32.1% of the MNZ-resistant strains had a rdxA mutation in G616A in the present study, which was slightly higher than the rate observed in a study in Pakistan (32.1 vs. 23.5%) (35). In addition, all strains (20/20) that were resistant to both CLA and LEFX were also resistant to MNZ in the present study. According to a study by Zhang et al (36), the rdxA mutation was identified in 90.7% (49/54) of Chinese children. Therefore, rdxA mutation detection in G616A may not be a good strategy to improve the eradication rate of H. pylori among adults in high MNZ-resistant areas, while it may benefit Chinese children.
As aforementioned, VacA is an important virulence factor in H. pylori. The association between VacA subtypes and H. pylori resistance remains controversial. Karabiber et al (15) demonstrated that VacA s1c-positive H. pylori was more likely to lead to CLA resistance, while in MNZ-resistant strains VacA s1 and s1c were more likely to lead to resistance. Wang et al (37) reported that the resistance rate of MNZ in the VacA s1m1/m2 strains was lower than that in the VacA s1m2 strain. However, Bachir et al (38) and Xu and Xie (39) demonstrated that the VacA genotype was not linked to H. pylori resistance. The present results indicated that there was no significant difference in H. pylori multidrug CLA, MNZ and LEFX resistance between VacA m1 and VacA m2 strains. The present results were consistent with studies by Bachir et al (38) and Xu and Xie (39). However, the s2 strain was not detected and the s1 subtype was not classified into s1a,s1b and s1c in the current study. Intermediate region i1, i2 and i3 were not detected.
Antibiotic resistance is divided into primary and secondary resistance (40). The antibiotic resistance rate increases in association with the number of therapy failures (38). The majority of patients included in the present study has received treatment before; however, due to the unknown antibiotic history of the patient, it is not possible to accurately calculate the primary and secondary drug resistance rates of H. pylori in Zhejiang, China, thus further research is required. The average time required to culture H. pylori and perform an in vitro drug sensitivity test is 1 week. However, it only takes 1 day to detect drug-resistant genes, which can save valuable time. In addition, Giorgio et al (41) demonstrated that reverse transcription-quantitative PCR on feces, which is non-invasive compared with the use of gastric mucosa, can be a useful tool for the detection of DNA sequence and antibiotic resistance-related gene mutations in H. pylori. A previous study used gene chip technology to detect the drug resistance of H. pylori in children, and the main mutation sites were similar to those identified in the present study (17). Studies focused on adults have only been used for the detection of single antibiotic resistance (42,43). The chip used in the present study covers five commonly used antibiotics. The results of gene detection were not identical to drug sensitivity test in vitro. At present, there is a lack of relevant clinical data to compare the accuracy of the two methods. However, a relevant clinical trial is in progress. There are numerous non-conventional rapid methods for antimicrobial resistance diagnostics, including whole genome sequencing (WGS), matrix-assisted laser desorption/ionization time-of-flight spectrometry, Fourier transform infrared spectroscopy and microfluidics technology. WGS is being expanded in China, while other methods require high purchase and maintenance costs and large space for equipment. Therefore, none of them can be widely carried out in the clinic (44).
In conclusion, VacA m subtypes were not linked to H. pylori antibiotic resistance. The detection of associated mutant genes C556G, A562T and A562G in PBP1 for AMX; T87A, A91G, G91T and G91A in gyrA for LEFX; 926-928 (AGA to TTC) and 926-927 (AG to GT) in 16S rRNA for TET; A2142G and A2143G in 23S rRNA for CLA; and G616A in rdxA for MNZ has a clinical application value in predicting the antibiotic resistance of H. pylori, particularly the resistance to CLA and LEFX.
Not applicable.
Funding: The present study was supported by Zhejiang Natural Science Foundation Committee (grant no. Q20H030019), Zhejiang Administration of Traditional Chinese Medicine (grant no. 2017ZKL010) and Zhejiang Province Science and Technology Hall (grant no. 2016C37033).
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
BL designed the study. JZ analyzed and interpreted data. JD analyzed data and wrote the manuscript. YH and LM performed experiments and revised the manuscript. All authors read and approved the final manuscript. JD and JZ confirm the authenticity of all the raw data.
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of The First Affiliated Hospital of Zhejiang Traditional Chinese Medical University (approval no. 2017-KL-054-02; Hangzhou, China). All patients provided written informed consent.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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Vermilion Energy Netherlands B.V. (Vermilion) has operated in the Netherlands since 2004. We are engaged in natural gas extraction from small fields, which is destined for use by Dutch households and companies.
In 2004, we established our head office at the gas treatment center in Harlingen, and by the end of the year, we had 10 employees. In 2014, we opened our second office in Amsterdam. Today, we have dozens of production locations throughout the Netherlands. Here, we extract natural gas from small fields, most located in the province of Friesland. The natural gas is sent from the production locations to gas treatment centers located in Harlingen, Garijp and Waalwijk, among others.View all our locations
Since 2004, Vermilion has grown into a reliable employer in the Netherlands, with more than 225 people now working for us directly – these are employees with an employment or hiring contract. We also work with Dutch (often local) companies when we need support, including technical maintenance, printing and facility services. Nearly 800 people work directly and indirectly for Vermilion in the Netherlands.
Our parent company is Vermilion Energy Inc., a Canadian publicly traded company. Vermilion was founded in 1994 as an oil and gas exploration and production company focused on assets in Alberta, Canada. Our name originates from Alberta's Vermilion Lakes, which are known for their reddish ochre waters. Western Europe has since become an important growth area, and Vermilion is currently active in eight other countries in addition to Canada and the Netherlands. Our core values are the same all over the world: Excellence, Trust, Respect and Responsibility.
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FromClover + Birch
This simplistic hammock is an alluring, purposeful addition to any playroom, nursery, or children's indoor space.
Your child will find comfort in the unique design that supports them as they move. The short width of the support bar allows the hammock to be used in a seated position as a chair, while the gathered fabric provides space for reclining, similar to a traditional hammock. The 100% cotton canvas fabric is supportive yet pliable - excellent for play.
Your little one will love curling up with a book, retreating for imaginative play, or relaxing within the gentle swinging motion.
The set includes:
One ceiling hook that supports 250 pounds.
One carabiner that supports 250 pounds.
One hammock chair ( 25' of rope to adjust as needed, support bar, cotton hammock seat).
- Crafted from 100% cotton canvas fabric.
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- The oak support bar measures 3' in length. Necessary mounting hardware is included.
- Hand wash with warm water and mild soap.
Returns and exchanges are accepted on this product within 30 days of the order date. Item must be returned unopened / unused, in its original packaging.


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Tag: inkblending
As soon as my friend Rebecca told me she was going to apply to retrain as a nurse, I knew she'd ace it. She's super caring and smart and of course she was offered a place everywhere she interviewed! So, obviously I had a card already made and waiting to go when she was offered […]
Alcohol ink floral cards
So, I'm jumping on the alcohol ink trend… I've been eyeing up beautiful alcohol ink cards for months, too nervous to try it myself, but it was my birthday recently, so I asked for a few basics to get me started. I won't go into the detail of how I created these backgrounds today, as […]


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9 December 2019
On the surface, Conrad Shawcross' sculpture retains the appearance of scientific rationality.
7 November 2019
Art News
A new public sculpture by the British sculptor, Conrad Shawcross RA, was unveiled today at Chelsea Barracks. This marks the first artwork to be installed as part of an ambitious programme of public art commissions created for London's newest neighbourhood.


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Lemon Pepper Pasta Salad by EatWell Exchange
Total Time
40 min
20 min
20 min
This veggie-packed pasta salad is loaded with crunch and bright, fresh flavors. It makes a perfect side dish for any gathering, especially in the summer when tomatoes, corn, and zucchini are at their peak. The recipe was developed by registered dietitians (and best friends!) Ashley Carter and Jasmine Westbrooks, who founded EatWell Exchange, which won the 2021 WW Wellness Impact Award. Eatwell Exchange works to decrease health disparities in low-socioeconomic communities while keeping culturally relevant foods on the table.
Wegmans Small shells pasta
8 oz
Fresh lemon juice
2 Tbsp
Olive oil
¼ cup(s)
Dried basil
¼ tsp
Dijon Mustard
1 tsp
Table salt
¼ tsp, plus more for seasoning
Black pepper
¼ tsp
Garlic powder
½ tsp
Fresh parsley
¼ cup(s), chopped
Uncooked zucchini
10 oz, chopped
Lemon pepper
½ tsp
Uncooked sweet yellow corn
1 cup(s), or 1 cup canned corn, drained
Grape tomatoes
2 cup(s), cut into halves
Uncooked red onion(s)
¼ cup(s), chopped, finely chopped
1 item(s), medium, diced
- Cook the pasta in a large pot of salted, boiling water, according to package instructions. Drain the pasta in a colander, rinse briefly with cool water and drain it again.
- In a medium bowl or jar, combine the lemon juice, lemon zest, olive oil, basil, mustard, salt, pepper, garlic powder, and chopped parsley. If using a bowl, whisk the ingredients together. If using a jar, seal the lid and shake until combined. Set the vinaigrette aside.
- Coat a medium nonstick skillet with cooking spray. Add the zucchini and lemon pepper seasoning. Sauté on medium heat until tender, stirring occasionally. Set aside and let cool.
- In a large bowl, combine the pasta, zucchini, corn, grape tomatoes, and red onion. Add the dressing and toss. Gently fold in the avocado to combine. Before serving, season with a pinch of salt.
- Serving size: 1 cup


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Ladies & Germs, Friends and Clients, I'm temporarily reduced to WIFI internet access after a major snafu on my faithful VAIO desktop (strictly a software problem). Until I can get the Geek Squad out here to fix it (and put in some more RAM while they're at it), I'll be working from my satellite office in Starbucks for limited hours. If you need to contact me, please give me a call on my cell. If you need my cell, drop me a note at the email-me link at the right and I'll get back to you as soon as possible. Or call my landline, which is still working, and is listed in the book.
UPDATE: Well, the current ETA is (AARGH!) February 2nd! Never fear, posts will keep coming though. I'm almost a month ahead, thank goodness. I knew there was some reason I did that.
On the up side, maybe without the Internet, I'll actually get some book making and writing done. Keep you posted.
UPDATE: Got the laptop hooked up to the intertubes, which is not an ideal situation, but certainly better than no intertubes at all. Now it's February 6th before the technician can come out. Grrrrr! But at least I'm online again.
UPDATE: The good news is that I managed to fix my drivers. The bad news is that I had to do a system restore to do it, and now I have to reload everything and reset all my preferences. Yes, I did to a backup and didn't lose anything but some of my fun little sounds and icons and cursors. Annoying, but not a tragedy, by any means. And that external harddrive I bought? That sucker was the best investment I've ever made. Get yerself one now, and back up everything you own every night.


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What Is Atal New India Challenge 2.0?
Did you know, your company could get assistance from the government for standing out of the crowd and delivering a meaningful impact? This assistance is provided under flagship programs of the government. These are the most important programs that aim at addressing the major challenges of the nation.
The Atal New India Challenge program was launched on 28th April 2022, by the Atal Innovation Mission. It is in accordance with the NITI Aayog. The aims and goals of Atal New India Challenge 2.0 are mainly based on technological innovations. The innovations which solve the State's sectoral challenges and are relevant to societal issues.
Aims Of The Program
Atal New India Challenge 2.0 is aimed at incentivizing innovations that fall under the areas that are critical for the growth and development of the Nation. Dr. S. Chandrasekar, Secretary, DST, assured that the government will support the innovations which are revolutionary and motivate the startups to work towards difficult challenges while launching Atal New India Challenge. He talked about the sectors which are in dire need of startups such as Agriculture and Health with other difficult sectors like Chemicals and Pesticides.
Atal New India Challenge 2.0 primarily aims at addressing the Commercialization Valley of Death which supports the innovators to grow through the risks of resource access for the testing, pilot, and market establishment. Atal India New Challenge pushes prototype stage innovations and the shortlisted startups get the support to reach the commercialization stage over a time period of around 12-18 months with funding up to INR 1 crore. Along with the funding, another associated support is also provided by the AIM innovation ecosystem.
The Atal New India Challenge 2.0 Program aims at 18 challenges mainly from these Sectors along with an open invitation category:-
- Agriculture – Millet
- Post
- Women Centric
- Drinking water and Sanitation
- Renewable Energy and Clean-Tech
- Education
Agriculture - Millet
Challenge 1
Primary Processing – A solution required to elevate the efficiency of millet processing viz. Dehulling, Separation, Polishing, etc. The solution should be such that it retains nutritional value while being effective at the same time.
Challenge 2
Decentralized Processing – Lack of machinery/ Infrastructure that enables decentralization process for small scale. It should be affordable and user-friendly for farmers, FPOs, and consumers.
Challenge 3
Shelf-Life Augmentation – Need a solution to elongate the shelf life of millets, processed grains, flours, etc. It should aim at making it on par with the competing crops.
Challenge 4
Supply Chain Linkages – A solution for market linkage that improves the supply of inputs (HYV Seeds, etc) and outputs (distribution, etc).
Challenge 1
Digital Address System – Require Use Cases Applications (APIs) that interact with digital address central infra in the upcoming time for diverse use cases of digital address.
Women Centric Challenges
Challenge 1
Innovation for Women Hygiene – Need Sanitary napkins that are low cost, eco-friendly and sustainable compostable. Along with that, innovation for the detection and recovery of UTI and toilet concept for women while traveling.
Challenge 2
Innovation for Women Safety – Safety Gadgets, better life-saving applications, and tech for safety for violence against women.
Challenge 3
Professional Networking Opportunities for women – 'women only' business networking app/self-help groups, support women-led startups.
Challenge 4
Innovation for working mothers – electric breast pumps which are silent and made for on-the-go and child safety-related innovations.
Challenge 5
Lifestyle of Rural Women – Easing out the life of rural women by developing innovation for water collection and supporting them in their knowledge of natural resources management.
Winners Get Up To INR 1 Crore Of Funding
The Atal New India Challenge program has rolled out great advantages for the innovators out there. Each winner of the ANIC is eligible for funding of INR 1 crore which is divided into 2 parts – Grant-in-AId and Equity Investment. The Grant-in-Aid is of up to INR 80 lakhs and is a non-returnable grant. The Equity Investment is up to INR 20 Lakhs and 2% of the equity of the startup must be held by the government.
Apply Today
Listen up, innovative startup owners! Are you ready to make the most out of this program? Gear up and apply before the deadline. Are you trying to figure out how to go about this? You can apply with the assistance of Egniol Services. Our professionals deliver a hassle-free procedure of application for your business.
Our professionals will be engaged in a start-to-finish process of your application. Contact Egniol services to know more about the other aspects like eligibility and timeline. Write to us or call us to apply for the Atal New India Challenge 2.0 program today.


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A graduate of the University of Portsmouth's Television and Broadcast course, Jake Wiafe, has won a Bafta as part of the Little Dot Studios team for their short documentary 'Missed Call'.
The 20-minute film is shot on an iPhone and tells the poignant story of a teenage boy, Jim, and his mother Victoria as they make the decision to contact the boy's father, who vanished from their lives a decade earlier.
The small indie company won the Bafta in the Short Form Programme category against stiff competition from a raft of documentaries made by the BBC.
'Missed Call' can be watched on YouTube.
Jake joined Little Dot Studios in 2017 and has been senior operations assistant on the 'Real Stories' documentary channel for the past year.
Alex Hryniewicz, Head of Owned Channels at Little Dot Studios, said: "Jake was instrumental in the success of our Bafta-winning film 'Missed Call', preparing the film for release on to the platform, producing editorialised cut downs for Facebook and IGTV and even stepping in front of the microphone to co-host the podcast interview with the film-maker and commissioning editor that preceded the Bafta win.
"He's a hugely talented member of the Owned Channel team and we're extremely lucky to have him working at Little Dot Studios."
Missed Call is directed by Victoria Mapplebeck, the mother in the film.
In her acceptance speech she said: "This was a tricky film, as you can see, because I'm both director and parent to the fantastic Jim, and that meant I needed ongoing support from this fantastic dream team of commissioners and editors and producers.
"I think it's proof — I hope the film is proof that small is beautiful, because I shot the whole thing on that phone."
BSc television and broadcasting course leader Charlie Watts said: "This is a lovely result for a very talented graduate, and it gives further validation of the quality teaching and learning that has taken place."


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Best Digital Marketing Training in Zirakpur
With the boom on the internet and the advancement of technology, it is hard to ignore the importance of Digital Marketing. This has also given rise to the demand for experts having knowledge of digital marketing. So, if you want to scale heights in your career by learning the power of digital marketing, it is essential for you to choose the right training institute. Well, this is where most students get confused. Having observed this puzzle, we have come up with a list of things you need to check before enrolling in Digital marketing training in Zirakpur.
7 Things to Check Before Joining Digital Marketing Training
No matter whether you are a graduate or a professional, Digital marketing can be learned by anyone. However, there are certain checkpoints you need to look for before enrolling in the course. Here they are:
Join Certified Institute
It is essential for you to join a center that is certified. For instance, you can look for an ISO 9001:2015 certified institute. Having training from such an institute ensures that you will get quality training while doing digital marketing.
Check Trainers' Experience
Trainers play an important role when it comes to learning a course. Thus, we suggest you check the experience of the trainers who are going to train you in Digital Marketing. Ensure that the trainers are not only highly-qualified but also are well-informed as well as well-experienced.
Pay Attention to Course Curriculum
It is necessary for you to check the syllabus you are going to cover during the training. Some institutes only give basic training to the students; however, many brags about providing an all-inclusive knowledge of the field while the course. Thus, it is a must for you to have an eye on the course syllabus beforehand.
Live Projects
Another point that needs your consideration is to check whether the institute allows you to work on live projects while doing digital marketing or SEO training in Zirakpur. If you would be working on live projects during the training, it would become easy for you to work independently as a freelancer upon the successful completion of the course.
Students Reviews
You can check students' reviews to know about the genuine feedback about the institute you are planning to join for digital marketing training in Zirakpur. Having a nudge on the reviews, you will get to know about the good and bad points of the institute, thus would be able to plan your enrolment accordingly.
Practical Exposure and Batch Size
Another point you need to check is whether the institute is offering practical training to its students or not. If only theoretical knowledge is being given to the students then there is no point to join that center. Also, there are many institutes providing training in big batch sizes. This will not let you grab the knowledge of the topics completely. So, we suggest you join an institute that is offering practical training to its students, especially in a one-to-one manner.
Last but not least, cost matters when it comes to joining a training institute. Not everyone can invest thousands of rupees for a short-term course. Thus, compare the cost of the course with various other institutes and join the one that best fits your needs as well as budget.
Having a check on these points prior to joining a training institute will help you make the most of your time and effort. So, what are you waiting for? Navigate to Google and find the best digital marketing training institute in Zirakpur keeping all the above points in mind. If you find this piece of writing of some help, share your feedback with us in the comments section.
Read More: Top Digital Marketing Career's for Students


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Life Sciences Organisation sets out the UK stall
October 2018
Life Sciences Organisation (LSO) is a perhaps little known mix of civil servants and private sector specialists that forms a healthcare, life sciences and bioeconomy cluster in the UK Department of Trade and Industry. From this position, as well as assisting the UK government to develop policies supporting life science businesses, LSO's primary purpose is to help UK life sciences companies to do business overseas and support foreign life sciences companies to invest in the UK.
Further to this remit, this month LSO has issued an extensive series of guidance notes providing an overview of the advantages of investment in the UK's life sciences sector, in particular in the following areas:
- Cell and gene therapy: develop new treatments in the UK
- Antimicrobial resistance: develop solutions in the UK
- Medical technology: partner with the UK to develop new products
- Cardiovascular disease: develop treatments and technologies in the UK
- Cancer: develop treatments and diagnostic technologies in the UK
- Infectious diseases: develop diagnostics in the UK
Each of these notes explains the financial investment in research and other projects that the government is committing to these areas of technology, as well as the key initiatives and infrastructure that are in place or being developed.
LSO is also involved in managing Brexit and the post Brexit domestic policy such as the life sciences Industrial Strategy and Sector Deal (see our earlier Synapse articles Sector Deal and Industrial Strategy). In setting out its stall for the UK life sciences sector, is also clear that LSO regards the availability of high quality research scientists and world-leading research centres as key assets of the UK life sciences offering beyond Brexit (see also our earlier Synapse article UK Life Sciences Prospects post-Brexit).
LSO is inviting those interested in commercial opportunities in the UK's life sciences sector to contact it at firstname.lastname@example.com.
If you have any questions on this article or would like to propose a subject to be addressed by Synapse please contact us.
Paul England
Paul is a senior professional support lawyer specialising in patents law in our London office.
"LSO's primary purpose is to help UK life sciences companies to do business overseas and support foreign life sciences companies to invest in the UK."


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When does punishment become excessive?
Question: Your recent series on punishment was thought-provoking. I agree children should have consequences when they misbehave. Nonetheless, would you please clarify when punishment becomes excessive? What is the line between reasonable and too much? When I was young, I was spanked with a belt on numerous occasions. I always felt, and still do, that they were completely unnecessary and over the top. Do you agree?
Answer: Absolutely! Belts, paddles, switches, and other nefarious variations on that general theme are dangerous and unequivocally unnecessary.
I don't have a problem with spankings per se. The best research (that is, research done by people who are dispassionate on the subject) consistently finds that contrary to the ideological myth, when spankings are occasional, moderate (two to four swats on the child's rear end), and administered by loving parents who spank with their hands only, they are not associated with psychological, behavioral, or social problems.
Then we have well-intentioned claims that the Bible instructs parents to spank with a "rod," but the biblical term "the rod of discipline" is clearly metaphorical. It refers not to beatings with sturdy sticks, but to parental authority that is reliable, righteous, just, and unequivocal. For more on that subject, interested readers are referred to the statement on spanking found on my website at johnrosemond.com.
Before I answer your first question, allow me to address a misunderstanding. I do not believe that successful discipline is a matter of properly manipulating consequences and I don't believe it's always necessary to respond to misbehavior with consequences. Consequences are overrated and often overused. The key to effective discipline is an attitude, a certain presentation style, not consequences or punishment. When parents act like their authority is legitimate, that they know what they are doing and why, children do what they are told. When parents explain, threaten, yell, plead, and the like, children take every opportunity to misbehave.
My recent series on punishment was not an apologetic for a punishment-based approach to discipline. I merely said that punishment is an essential aspect of an effective disciplinary approach. Research — again, studies done by dispassionate individuals — confirms that assertion.
It's important to note that the "size" of a punishment does not determine whether it is excessive or not. That is determined by the parent's attitude. Punishment is likely to be excessive when the parent is angry and using punishment as a form of "payback." The parent in question is being impulsive and vengeful as opposed to calmly corrective. Whatever message the parent intends to send is blurred by his or her emotional reaction to the child's misbehavior.
Punishment that is driven by emotion accomplishes nothing and serves only to elicit emotion from the child. It accomplishes nothing of value; therefore, by definition, it is excessive.
Visit family psychologist John Rosemond's website at www.johnrosemond.com; readers may send him email at email@example.com; due to the volume of mail, not every question will be answered.


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Visiting sunflower fields is one of my favorite ways to celebrate the end of summer and the beginning of fall. Sunflowers in Cincinnati are essentially one huge field of summer's last hurrah!
The sunflower fields and farms are a wonderful place to walk and take in all the season has to offer. Be sure to bring the camera as this late bloomer holds nothing back and the sunflower fields make for a wonderful backdrop for family pics. And a few of the farms will even allow you to cut a few to take home.
Get out there and enjoy our list of sunflower field favorites! Let us know if we missed YOUR favorite by leaving a comment below.
Sunflower Fields Near Cincinnati:
🌻 Blooms and Berries has some of the best fall fun around!
Fall on the Farm is their big fall event. It includes their beautiful sunflower fields and it's a must-do for our family each fall.
Sunflowers at this farm are expected to be blooming during the end of September into the beginning of October.
Fall on the Farm dates are September 17 – October 31, 2022.
Be sure to grab your tickets online before your visit.
🌻 Burwinkel Farms has acres of sunflower fields with walking paths, a pumpkin patch, and a corn maze and lots more fall fun. Be sure to check with the farm for their peak weekends.
They have some early bloomers in July of 2022 and will have more sunflower crops later.
Watch for updates on their Facebook page.
🌻 Country Pumpkins is having their Sunflower Days from August 12 through August 26, 2022.
This 250 acre dairy farm is located in Dry Ridge, Kentucky. Take a shuttle ride out to their sunflower field where you can take all the selfies you want!
Timed tickets are required (buy online). No sunflowers are included in your ticket but you can add on U-Pick sunflowers to your ticket.
🌻 Gorman Heritage Farm is having their annual Sunflower Festival – October 1 and 2, 2022.
Explore the beautiful historic working farm including several miles of wooded trails, meet the farm animals, buy pumpkins, and, of course, sunflower fields. They'll also have an opportunity for you to cut your own sunflowers ($1 per stem), food trucks and vendors on site.
Admission: $10 adults, $5 kids ages 3-17, $5 adults 60+, kids under 3 are free.
Full details can be found here. Grab your tickets online (no tickets at the gate).
You might also like: Best Fall Festivals in the Cincinnati Area
McGlasson Farms in Hebron, Kentucky should have sunflowers in late September/ early October.
It's a perfect fall weekend! U-pick pumpkin patch plus sunflowers, apple cider, caramel apples, fall squash, corn stalks, sweet potatoes, sweet corn, tomatoes and more!
Cash or check only for this farm.
Watch their Facebook page for updates.
Schappacher Farms hopes to have multiple patches of sunflowers this year!
They plant sunflower fields on the late side so you can admire their sunflowers when the pumpkins are ready too.
The Meade Historic Preserve in Symmes Township has a field that blooms once a year.
This park is free and they encourage you to come visit to see the sunflowers.
Head over to Symmes Twp. Parks for info.
It looks like Niederman Family Farm is in on the sunflower action for 2022!
They have sunflowers in mid-July this year.
Vogel Farm in NKY has lots of sunflowers and will often post (to their Facebook page) about times when they have the fields open to the public (usually starts in mid-July).
They specialize in cut sunflowers and bouquets which they sell at their farm stand.
They just posted that the sunflowers fields are planted and doing great!
Be sure to "like" their Facebook page to get the open field updates. Bring cash!
The Black Barn in Lebanon usually has a one acre sunflower field. The 2022 field info has not been announced yet.
plants a sunflower field in Mason, found near Cottell Park in Deerfield Township, and it is typically one of the earliest to bloom in Cincinnati. This field of flowers is free and open to the public but they do ask that you respect the field, don't pick the flowers, and park at Cottell Park.
Sadly, it looks like the sunflower fields won't be planted in this location any longer. Natorps says: "Sunflower Field Update: Many of you know the land is leased from a farmer who generously allowed Natorp's to plant sunflowers. Now, after many years he would like his land private for farming. This means saying goodbye to the sunflowers. Thank you all for sharing your wonderful photos and kind words throughout the years."
Like all flowers, sunflowers bloom for a short time. It's always a good idea to check the status of the sunflower fields before you head to the farm.


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Antivirus programs can clean mobile or spyware and force away spyware and malicious applications that try to gain access to your phone. Although many antivirus software are not ideal, a few be different above the rest. That they don't require intense bandwidth or perhaps performance issues, and they surpass in reading user reviews and feature set. Listed below are among the better antivirus apps for Android os. Choose one of these antivirus apps today! But how would you know which one is https://beastapps.net/avast-business-antivirus-review right for your device?
Avira can be described as relatively modern antivirus iphone app, but has exploded rapidly yesteryear. The totally free version of this app contains basic features like virus verification and personal privacy scanning. In addition, it offers gadget admin features, anti-theft support, and level of privacy scanning. It really is more light than Norton, and is sold with three tiers of premium features. The paid variant has more advanced features and a every year license that will cost $5. Kaspersky Mobile Anti-virus is an excellent decision if you're searching for a free anti virus app.
McAfee is another good choice for iOS devices. That is simple to use and comes with a availablility of security features. It filtration spam texts, protects you against malicious websites, and spots vulnerabilities within your device. The app as well protects you Wi-Fi scratches and is helpful for keeping track of your device if you lose it. Additionally, it has a remote alarm feature and media channels vault, which automatically backs up contact information.


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Balasubramanyam, M and Premanand, C and Mohan, V (2002) The lymphocyte as a cellular model to study insights into the pathophysiology of diabetes and its complications. Annals of the New York Academy of Sciences, 958 . pp. 399-402. ISSN 0077-8923
Blood cells from subjects with hypertension and/or diabetes mellitus have been successfully studied in the past to gain insight into pathological alterations of several signal transduction pathways. Diabetes mellitus is also considered to be a disease of abnormal cellular Ca2+ metabolism, as metabolic derangements of Ca2+ transport have been noticed both in the prediabetic state and as a consequence of hyperglycemia and oxidative stress. In this report, we used peripheral blood lymphocytes from type 2 diabetes patients and control subjects to study and delineate different mechanisms of Ca2+ turnover that determine the level of cytosolic calcium (Ca(i)). While demonstrating the specific Ca2+ turnover alterations, we suggest that insights into the pathophysiology of diabetic complications originating from signal transduction defects could be conveniently studied using blood cell types such as lymphocytes and that such studies could lead to the identification of new molecular drug targets.
|Item Type:
|Official URL/DOI:
|Uncontrolled Keywords:
|diabetes;pathophysiology of diabetes;The lymphocyte;cellular model
|Diabetes > Diabetes Awareness and Prevention
Biochemistry,Cell and Molecular Signalling > Pathophysiology-Diabetes
|Department of Cell and Molecular Biology
Department of Diabetology
|ID Code:
|Deposited By:
|Deposited On:
|23 Nov 2009 11:32
|Last Modified:
|23 Nov 2009 11:32
|Related URLs:
Repository Staff Only: item control page


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Browsing Artículos científicos by Subject "Maqui"
Now showing items 1-1 of 1
Maqui (Aristotelia chilensis [Mol.] Stuntz) morphological and phenolic traits associated with forests type and latitudinal gradient in natural populations of Patagonia Argentina (Elsevier, 2021-12)Maqui (Aristotelia chilensis [Mol.] Stuntz) is a native berry of the Patagonia Andean forests, which has one of the highest antioxidant levels currently known. This biochemical characteristic strongly depends on the phenolic ...


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Home urq20v.com
This is what the floor mat anchors look like under the carpet.
Seems like an after though, just rivited to the floor.
However it does make it easier to add to my car.
I figured it might be a good idea to measure and take some
pictures of where they are attached.
All measurements are in inches.
I'm not even sure if the floor pan looks the same on the urq.
When I install them I'll take some additional photos.


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Examining the Link between Children's Cognitive Development and Touchscreen Interaction Patterns
Ziyang Chen, Yu-Peng Chen, Alex Shaw, Aishat Aloba, Pavlo Antonenko, Jaime Ruiz, and Lisa Anthony. 2020. In Proceedings of the 2020 International Conference on Multimodal Interaction (ICMI '20). Association for Computing Machinery, New York, NY, USA, 635–639. DOI:https://doi.org/10.1145/3382507.3418841
Designing Natural User Interfaces for Children
In collaboration with Dr. Lisa Anthony's INIT Lab, this work explores the challenges children have interacting with Natural User Interfaces (NUIs), i.e., interfaces that use touch, speech, and gesture. Children are increasingly being presented with technology, such as tablets and smartphones, in classrooms, museums, and at home. However, children are still developing their cognitive and physical capabilities, and prior work has shown that children's interaction behaviors and expectations are different from those of adults. For example, our prior work has shown that gesture recognition algorithms do not perform as well for children, mainly due to their motor skills development affecting their writing ability. Our goal is to build better technology and interactions for children by understanding how children interactions differ from adults.
In our work, we have examined children's touch and gesture interactions on smartphones, tablets, and tabletops. On the tabletop, children responded more accurately to changing target locations and were more accurate touching targets around the screen. We also found that the gesture recognition rates were consistent across devices, which implies that gesture data does not have to be collected on the device that recognition will take place. Throughout our work, we provide design guidelines for children's touchscreen interactions to inform the design of touchscreen applications for children. While our work has mainly focused on improving recognition and accuracy, we also examined children's expectations of intelligent user interfaces that use modalities such as speech and writing. We developed a conceptual model of the children's expectations which can be used along with our prior work on improving accuracy to further develop technology that is tailored towards children. We continue to build upon our work to further examine children's expectations and abilities in respect to Natural User Interfaces.
Examining the Link between Children's Cognitive Development and Touchscreen Interaction Patterns
Human-Centered Recognition of Children's Touchscreen Gestures
Alex Shaw, Jaime Ruiz, and Lisa Anthony. 2017. In Proceedings of the 19th ACM International Conference on Multimodal Interaction (ICMI 2017). ACM, New York, NY, USA, 32-40. DOI: https://doi.org/10.1145/3136755.3136810
Tablets, tabletops, and smartphones: cross-platform comparisons of children's touchscreen interactions
Julia Woodward, Alex Shaw, Aishat Aloba, Ayushi Jain, Jaime Ruiz, and Lisa Anthony. 2017. In Proceedings of the 19th ACM International Conference on Multimodal Interaction (ICMI 2017). ACM, New York, NY, USA, 5-14. DOI: https://doi.org/10.1145/3136755.3136762
Using Co-Design to Examine How Children Conceptualize Intelligent Interfaces
Julia Woodward, Zari McFadden, Nicole Shiver, Amir Ben-hayon, Jason C. Yip, and Lisa Anthony. 2018. In Proceedings of the 2018 CHI Conference on Human Factors in Computing Systems (CHI '18). ACM, New York, NY, USA, Paper 575, 14 pages. DOI: https://doi.org/10.1145/3173574.3174149
Investigating Separation of Territories and Activity Roles in Children's Collaboration around Tabletops
Julia Woodward, Shaghayegh Esmaeili, Ayushi Jain, John Bell, Jaime Ruiz, and Lisa Anthony. 2018. In Proceedings of the ACM on Human-Computer Interaction, Vol. 2, CSCW, Article 185 (November 2018). ACM, New York, NY. 21 pages. https://doi.org/10.1145/3274454
Examining Fitts' and FFitts' Law Models for Children's Pointing Tasks on Touchscreens
Julia Woodward, Jahelle Cato, Jesse Smith, Isaac Wang, Brett Benda, Lisa Anthony, and Jaime Ruiz. 2020. In Proceedings of the International Conference on Advanced Visual Interfaces (AVI '20). September 28– October 2, 2020, Ischia, Italy. ACM, New York, NY, USA, 5 pages. https://doi.org/10.1145/3399715.3399844
"It Would Be Cool to Get Stampeded by Dinosaurs": Analyzing Children's Conceptual Model of AR Headsets Through Co-Design
Julia Woodward, Feben Alemu, Natalia E. López Adames, Lisa Anthony, Jason C. Yip, and Jaime Ruiz. 2022. In CHI Conference on Human Factors in Computing Systems (CHI '22), April 29–May 05, 2022, New Orleans, LA, USA. ACM, New York, NY, USA, 13 pages. https://doi.org/10.1145/3491102.3501979


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Apstein: Before ruling baseball, Scott Boras failed at baseball
From Stephanie Apstein at Sports Illustrated on May 13, 2020:
Before Scott Boras ruled baseball, he was a 24-year-old second baseman in Double A, and he was fast asleep. A knock on his hotel room door awoke him around 6 a.m. "Boras!" rumbled Cardinals minor league field coordinator George Kissell. "Get up!"
Boras's mind raced. He was scorching the ball for the Arkansas Travelers. He had just come off a two-hit night. He was imagining his call-up by the time he turned the knob.
"Put some clothes on," Kissell said. "We're going to church."
"George," Boras pointed out, "it's Tuesday."
"I know," Kissell said. "But I saw you play defense last night, and we're going to church and praying that this workout's gonna make you better defensively."
Read the full article here: https://www.si.com/mlb/2020/05/13/scott-boras-baseball-career
Originally published: May 13, 2020. Last Updated: May 13, 2020.


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31 Main St (Main St/Prince Edward St)
Price: $1,958,888
Status: Sale Pending
- Tax: $16,153 (2022)
- Community:Brighton
- City:Brighton
- Type:Residential
- Style:Multiplex (2-Storey)
- Beds:8
- Bath:4
- Size:5000+ Sq Ft
- Basement:Full
- InteriorFireplace
- ExteriorBrick, Vinyl Siding
- HeatingBaseboard, Gas
- Sewer/Water SystemsPublic, Sewers, Municipal
Listing Contracted With: ROYAL LEPAGE MEADOWTOWNE REALTY, BROKERAGE
*Calling All Investors Over 7% Cap Rates!* Prime Mixed Use Investment Property Located In Downtown Brighton Consists Of Commercial On Main Level W/3 Units (Leased & Willing To Stay). Upper Level Consists Of 4 Residential Units (Leased W/Tenants Willing To Stay). Drawings For 5 Additional Residential Units Also Included. Possibility For A Third Storey. Units Separately Metered. Building Boasts 14,000 Sq Ft Over Two Levels. Excellent Opportunity For Owner/User Or Investor. Building Has Been Completely Renovated (New Windows/Doors, Metal & Membrane Roof, Hot Water Tanks, Furnace, Ac, Insulation. Very Well Maintained Property With Tons Of Future Potential. Core Commercial Zoning Has 12M Height Restriction W/ 75% Lot Coverage, Permits Hotel, Banquet Hall, Apartments & Many More Uses. Pls Review All Attachments.
Alarm System, Carbon Monoxide Detector, Hot Water Tank Owned, Smoke Detector, 4 Refrigerators, 4 Stoves, 1 Coin Operated Washer, 1 Coin Operated Dryer. Exclusions: Tenant Belongings.
Want to learn more about 31 Main St (Main St/Prince Edward St)?
Denise Dilbey
Real Estate Broker
Royal LePage Meadowtowne Realty, Brokerage