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Millennials Are The Future… Are Your Logistics Operations Ready For Them?
With millennials currently making up around one-third of the workforce and looking set to account for three-fourths of the workforce by the year 2025, suppliers that want to remain relevant need to make sure they master the best ways to work with this group of people.
One thing that sets this generation apart is the incredibly rapid pace at which information is shared. They seek real-time information for decision making, and every firm should review its organizational capabilities with this in mind.
What is important to millennials? First and foremost, they crave positive feedback much more than monetary rewards. They are also highly concerned about ethics, so supplier conduct is of the utmost importance. They also favor short meetings and quick emails over long business lunches. It's important to keep them apprised of what is going on at every moment. Provide regular updates, and allow them to access information about operations in real time on their own as needed.
Constant Availability And Rapid Responses
Many businesses will find that millennials operate on a 24/7 work cycle thanks to handheld devices, so speedy replies are crucial. In addition, suppliers must be prepared to offer innovative solutions and ideas. Don't be surprised when they ask for changes or challenge the status quo, and be prepared to provide a workaround while better solutions are bring implemented.
You should also be ready to provide references for similar projects or business types you've worked with in the past. One of the best approaches that can be taken with this generation is to under-promise and over-deliver. Pleasantly surprising clients can go along way toward securing repeat business and cultivating long-term relationships. You'll want to build trust and loyalty, just like you would with any other type of client. Above all, however, suppliers that want to stay afloat with the next generation of clients need to focus on transparency and real-time data.


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Lucy Beishon
Research Training Fellow
I have been working at the University of Leicester as a Clinical Research Fellow in Geriatric medicine, with an interest in cognitive disorders (i.e. dementia). I am particularly interested in how brain blood flow is altered or damaged in dementia and how this contributes to the development or progression of different types of dementia.
I am currently funded by the Dunhill Medical Trust on my research training fellowship project entitled 'The Cognition & Flow Study: The effects of brain training on brain blood flow.' This study aims to investigate the use of cognitive or "brain training "as a treatment for Alzheimer's disease, and mild cognitive impairment, and as prevention in healthy older adults. This study will assess a range of outcomes, including mood, quality of life, and ability to carry out daily activities. The study will also look for any effects of brain training on brain blood flow before and after cognitive training to identify if blood supply to the brain can be improved in dementia by stimulating the brain on a regular basis with cognitive training. The study will be completed with a series of interviews to identify any benefits or difficulties with the programme that are not captured by the outcomes listed above.
In addition to my research work, I am also a specialist trainee in geriatric medicine and my long-term goal is to work as a geriatrician with clinical and research interests in dementia. In the future I plan to investigate the role of cognitive training, and the effects on brain blood flow in dementia, in a larger multi-centre study.
The Dunhill Medical Trust
5th Floor
6 New Bridge Street
London EC4V 6AB

subset: commoncrawl


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Do not be angry with me for speaking the truth; no man will survive who genuinely opposes you or any other crowd and prevents the occurrence of many unjust and illegal happenings…. A man who really fights for justice must lead a private, not a public life, if he is to survive for even a short time. (Plato's Apology 31e-32a)
Democracy – which can be defined as that system of government whereby all eligible citizens cast a ballot and vote directly for their leadership – has been around since at least Plato's Athens, around 300-400 BC. The Great Greek's discussions on politics, found mostly in Timaeus (start reading at section V), Republic and Apology , paint a sorry picture for the possibilities of any purely democratic system. In fact, Plato finds democratic government to be the worst of all possible governing types, with the exception of tyranny (rule by a dictator). For great thinkers like Plato (Socrates, Avicenna, whoever), then Aristotle, down to our very own John Locke, it has been routinely held that rule by the common people [demos=people + kratos=rule] is equivalent to Mob Rule, even worse than rule by timocracy (the Good Ol' Boy syndrome), aristocracy (a king or queen and their line), and even rule by money (oligarchy).
Just briefly I want to explain why Plato and many of his time felt the way they did about democracy as this can help in understanding why we need a new political party to challenge the two-headed beast we now have. Plato's reservations with accepting democracy had nothing to do with its implementational viability or things like people not voting and so on. Rather, Plato had a view of common human nature that was not so flattering and this was the basis for the condemnation. The sheep, let me call them for Plato, simply cannot as a rule be trusted to make the important decisions necessary to run a nation, state, or even city. The reasoning he supplies, and which I think today remains legitimate, is essentially two-fold: first and most importantly, the common sheep are neither properly raised nor adequately educated; secondly, the sheep are very susceptible and gullible, easily swayed by bad logic, psychological manipulations, blind emotion, and we should add today, advertisements and the media. Plato is often taken to task for his views on censorship (many people simply can be made crazy by presentations…), but this derives from the same theoretical base. The common people, or as I say sheep, simply cannot be trusted to be reasonable. Uneducated as they are they cannot keep their emotions under control, or be trusted to make the right choices. Our history of copycat crimes based on cinema and television broadcasts, and the alleged efficacy of marketing strategies can alone attest to the ease with which the sheep can be manipulated. People can supply reasons for their choices but hardly ever rational ones.
But there seems to be a light at the end of Plato's democratic tunnel-vision, and that is that IF – and I cannot stress this word enough because it still has not come to pass – IF the people were raised rightly, and educated correctly, THEN a democracy could stand a chance. In America today it is often thought that we have a democracy but we do not. Our founding fathers were well aware of classic ideas of government and this, coupled with their underlying dissatisfaction with the then-current aristocratic government (which had by then devolved into timocracy and oligarchy), was all they needed to be sure and qualify the new American democratic process. The framers of the Constitution wanted to combine the voice of "the people" (those not in the aristocracy) yet keep the classical idea of aristos, or the best. What resulted was a hybrid we still have today that is known as a "representative democracy" and makes it so that the people vote on few issues directly and for the most part use their voting to elect their representatives, seemingly still, chosen from the "best."
The problem is the meaning of "best." Clearly the great thinkers did not define "best" as the one who could make the most money, speak the most appealing things to the masses (see especially Sophist), or look the best on the screen. "Best" for the ancient Greeks meant superior in reasoning ability, and possessed of the Good in every aspect of life. The best man is therefore the most righteous man, the fairest man, the honest man. In Plato's time, we must remember, democracy was the rule in Athens even though he lobbied against its merits. It was after all democracy, the voice of the people, that also condemned his beloved Socrates. The early proponents of democracy knew its limitations, and that it would be difficult for it to succeed in any polis much bigger than Athens, which in 300 BC was already experiencing some of those problems. So, for a democracy to work, in theory, the people need to be educated, and the population must be small enough so that everyone is adequately "spoken for," either by direct vote or through a representative.
We still have not yet any of this basis for democracy's success. The people, while most go to school, are scarcely educated, and most come from single or divided-parent households where their early upbringing leaves much to be desired. We do, in a sense, still need this Republic, we need still representatives for those who cannot, or are unable to represent themselves. This then electing of congressmen becomes a key issue, the selecting of each one as important for that electorate as is the President for the nation. But electing Senators and Representatives today we find ourselves in a similar circus. While everyone fitting the qualifications may toss their hats in the ring, it is a known fact that only those with enough money (oligarchy) and connections in business and the media (timocracy, cronyism. lobbies) encounter any kind of success. Because the area of coverage each representative is meant to speak for is so large (over 700,ooo to 1 in Texas…), the constituents so many, only those with big enough bank accounts and quick dial lists ever really get a chance to compete. The smaller-budgeted prospective candidates are never even seen.
With this post then I introduce a step in the direction of getting better people for the jobs of politician seen. The people of America need an alternative voice with power and a defensible platform to challenge the existing dynamic (via dunamis, also Greek, for always moving…) duo we call contemporary politics, the maddening red and blue two-sided slug of a coin. That is, I introduce with this essay what we will for working purposes (we can change the name later if deemed beneficial) call the Common Sense Party. IF there are to still be these political parties, we surely need another one, a real one with a chance to win. Perhaps a union of the existing alternative parties deserves some discussion.
This new political foundation will be followed-up on in our forum, in the members' section, and anyone who finds it interesting is encouraged to share the idea and participate in refining the platform, which I will begin presently. We can also give it a working title, the Neo-Declaration of Independence.
1. No representative at any level of government, be it mayor of a town or President of the nation, may make more at his position that the median income of his constituency.
2. No government benefits shall be paid for retired politicians at any level.
3. We demand regulation on any goods and services for which the people have no choice or no real choice. Examples are:
a. Mandatory insurance of any kind
b. Electricity, gas, and other fuels and energies
c. Local telephone, internet, cable
d. Health care and prescription costs; no capitalizing of any kind on illness
e. and more
4. We must stop being the police of the world. We simply cannot afford it anymore.
5. We must reduce the military expenditures by half in peace time. Too much business is involved.
6. We must push for peace at all costs and use war only as a last resort. This includes cold wars, covert wars, propaganda wars.
7. Any US military intervention without a declaration of war shall be ended immediately.
8. 30 years of service shall now be required to receive any government pension.
9. Basic health care must be immediately given to every US citizen as a benefit of citizenship, paid for by a combination of taxation and personal annual contribution based on income.
10. We must discuss some sort of basic intellectual proficiency for any voter. If you can't add 5 plus 3 and get the right answer then you shouldn't be voting. But his is just a wish…
11. We must support national decriminalization of marijuana and all cannabis products. The current government position of ruining lives and putting people in jail for no crime is absurd and in fact more detrimental to society than the behavior it cannot possibly prevent. The current stance is symbolic of governmental, scientific, and political incompetency and continued telling of outright lies. No crime should be a crime in America if there is no conceivable tort, that is, harm accrued by anyone else. Which leads to:
12. We must push for a re-affirming the person's right to his or her own self, that each individual should be free from molestation from without, and free to even choose to die if the circumstances so warrant.
13. We are against abortion but FOR a person's right to have one. Given #12 above, it is your right to do with your own body what you will. But for me, it's just become too easy and should be reserved for extreme cases. There is also that debate about that body inside of the pregnant woman. It is with sad face I would have to keep those clinics open. But such is the nature of freedom to choose. Bottom line is government cannot pay for it in any way.
14. All contributions to politicians shall be recorded and must be INDIVIDUAL only. No corporate contributions, and there must be a strict limit imposed for each social security number.
15. The media must be forced to donate time to all candidates of every (even minor) party equally.
16. Commercials and advertisements for politicians must be screened for accuracy before they are presented to the public.
17. We need to audit the Federal Reserve (or close it), trim the administrative section of the budget, and review all government contracts (n.b. under FINANCIALS).
18. There will no longer be any such thing as a "non-profit organization"; so long as any organization, committee, fellowship, etc. has people on the payroll, that organization shall be taxed as earning a profit.
19. Corporations shall be held to scale-based taxation off the gross receipts, not the bottom line. Too much is dumped to avoid such taxes. Members of the corporations shall be held accountable for corporate liabilities.
20. We shall eliminate the Port Authority, the Federal Reserve Bank, and any more bailing out of private enterprises.
21. We shall erect a National Bank Of The People to challenge the private options. This will be non-profit, after expenditures from the excess income from loans and so on going to establish new branches.
22. We will discuss closing all stock markets, or regulating them and taxing them, like the high stakes casinos they are.
23. We'll discuss an exchange of cleaner tobacco in exchange for lower tax rates.
24. Taxation needs a complete overhaul. As is the little people pay too much while the big bucks boys do not pay enough. Too much of the dollar sent to the government – at all levels – goes to salaries for employees and to waste.
25. Our stimulus for growth? America needs to make things again, things cheap enough and of good enough quality that the whole world will want to buy them again. Real working production jobs. Co-ops, where the people who work at the factory reap the profits of that factory equally, is the best idea we have heard yet.
Well, there you have it, 25 points to send you sheep ascurry. Help us fix it, or get out of the way.
Who's responsible for change?


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The truth is, it isn't easy to change your mindset all in a few months. You can read the books, do the work, practice the prayers and meditations. But there are going to be setbacks. I had one of my own this week, I had to really plug in to my practice and the universe in order to re-evaluate the situation.
I'm have a part-time job at the Gap in a neighboring town. I'm taking a semester off from school, but I'm going back in January. I live with family and I don't have to pay rent or for food, which is an amazing set up. But around the holidays, my co-workers increase and my hours decrease. So, yes, my paychecks have literally been cut nearly in HALF. And I had a minor freak out.
"I just set up my budget and now I can't make it."
"I have no self-control over spending money."
"I suck at saving anything more than a few dollars a month."
All of these and more negative, fear-oriented thoughts FLOODED my brain. And I went crazy trying to figure out how to make a little extra money so I can have all the things I think I need and be successful in my life.
And this morning, after days of considering the MOST ridiculous options (it's embarrassing, so I won't get into it!), I finally had a breakthrough. I remembered once again that MONEY ISN'T EVERYTHING. And what I can GET is NEVER going to be as good as what I can GIVE.
MONEY will not make me HAPPY. MONEY will not make me SATISFIED in my personal life. THESE THINGS are not the answer. And now I've come home again, back to my place of love and giving and peace. I've accepted and forgiven myself for my fearful thoughts and I'm changing them into something I can really believe in.
"I have a budget for a reason, and I can follow it."
"I think hard about my purchases and use my money wisely."
"I save up as much money as I need each month."
Have a beautiful day. xx.


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Volunteer deliverers are collecting their copies of October's "The Connector" ready for delivery across the estates. The print for this edition was raised to 2200 copies in an effort to ensure there is a copy for each and every home.
Thanks goes to our volunteer deliverers. You can contact our secretary on email@example.com or call 0498 266 059 if you are available to help out as a volunteer deliverer. Deliverers are asked to deliver between 100 and 200 copies and this usually takes around 1 hour to do.
You can download your own colored copy from HERE. It is a little over 3MB in size.
Or you can peruse the compressed pdf version below. For a "full page view" click on the box in the bottom right hand corner with the four arrows, each arrow is pointing to a corner. The page opens in a new tab. To exit the full view click on the "X", top right hand corner to return to this page.


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Kirsten Dunst is set to star in a dark comedy series "On Becoming a God in Central Florida" in development at AMC with George Clooney executive producing, TheWrap has confirmed.
Dunst, who will also executive produce, will star as Krystal Gill, a recent widow and Orlando water park employee who schemes her way to the top of a pyramid scheme called Founders American Merchandise, which previously bankrupted her. The series will take place in the early 1990's.
Clooney is producing along with Grant Heslov under their Smokehouse Pictures banner. "The Lobster" director Yorgos Lanthimos will direct and executive produce. Robert Funke and Matt Lutsky wrote the project and will also executive produce. TriStar Television will produce along with AMC Studios.
See photos: Every 2016 'Walking Dead' Episode Ranked From Worst to Best
See photos: Every 2016 'Walking Dead' Episode Ranked From Worst to Best
An individual with knowledge of the project said that it is in the very early development stages at this point.
Dunst recently starred in Season 2 of the hit FX series "Fargo," in which she played Peggy Blumquist, a Midwestern housewife and hairdresser with delusions of grandeur.
Smokehouse has produced several of Clooney's recent films, including "Money Monster," "The Monuments Men" and "The Men Who Stare at Goats." The company also produced the television series "Memphis Beat."
Also Read: AMC-Carmike Merger Gets DOJ Approval, Will 'Almost Certainly' Close Before Year-End
Also Read: AMC-Carmike Merger Gets DOJ Approval, Will 'Almost Certainly' Close Before Year-End
Dunst is represented by UTA and Management 360. Smokehouse, Funke, Lutsky and Lanthimos are represented by CAA.


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In this study, we used PCR typing methods to assess the presence of tetracycline resistance determinants conferring ribosomal protection in waste lagoons and in groundwater underlying two swine farms. All eight classes of genes encoding this mechanism of resistance [tet(O), tet(Q), tet(W),tet(M), tetB(P), tet(S),tet(T), and otrA] were found in total DNA extracted from water of two lagoons. These determinants were found to be seeping into the underlying groundwater and could be detected as far as 250 m downstream from the lagoons. The identities and origin of these genes in groundwater were confirmed by PCR-denaturing gradient gel electrophoresis and sequence analyses. Tetracycline-resistant bacterial isolates from groundwater harbored the tet(M) gene, which was not predominant in the environmental samples and was identical to tet(M) from the lagoons. The presence of this gene in some typical soil inhabitants suggests that the vector of antibiotic resistance gene dissemination is not limited to strains of gastrointestinal origin carrying the gene but can be mobilized into the indigenous soil microbiota. This study demonstrated thattet genes occur in the environment as a direct result of agriculture and suggested that groundwater may be a potential source of antibiotic resistance in the food chain.
The widespread use of antibiotics in humans and animals has raised several concerns related to human and animal health. The principal area of concern has been the increasing emergence of antibiotic resistance phenotypes in both clinically relevant strains and normal commensal microbiota. In two recent studies, a link between the agricultural use of antibiotics and antibiotic-resistant human infections has been suggested (24, 33). Because consumption of tainted food is considered the main route of transmission of drug resistance, other possible means of antibiotic resistance dissemination have received relatively little attention. One of these possible means could be natural water and soil environments impacted by antibiotics and antibiotic-resistant bacteria from agriculture, where antibiotics are used for disease treatment, prophylaxis, and growth promotion. The concern over the use of antibiotics in agriculture, especially for prophylactic and growth-promoting purposes, has not been limited to the presumed role of antibiotics in selection of antibiotic-resistant bacteria (pathogenic or nonpathogenic) in the animal gut. The more debatable issue arising from chronic low-level exposure to antibiotics is whether this practice contributes significantly to increased gene frequencies and dissemination of resistance genes into other ecosystems.
Furthermore, many antibiotics used in animal agriculture are poorly absorbed in the animal gut. It is estimated that 25% to as much as 75% of the antibiotics administered to feedlot animals could be excreted unaltered in feces (6, 7) and can persist in soil after land application (4, 11). There is little information available concerning the fate of antibiotics in the environment and their link to the emergence of resistant genotypes found there. The annual production of livestock and poultry waste in the United States is nearly 180 million tons (dry weight basis) (13, 34), and coupled with antibiotic usage, this waste is a potentially large source of both antibiotics and antibiotic-resistant bacteria released into the environment.
Lagoons and pit systems are typically used for waste disposal in animal agriculture. Seepage and runoff into watershed systems are of particular concern due to potential mobilization of constituents and exposure of contaminants to humans and other animals. Groundwater, in particular, constitutes about 40% of the water used for public water supplies and provides drinking water for more than 97% of the rural population in the United States (http://water.usgs.gov/wid/html/GW.html ). Recent monitoring studies have demonstrated the vulnerability of groundwater to seepage from waste lagoons (19). Over a period of several years, Krapac and coworkers found indicators such as ammonia and fecal enterococci at elevated levels in groundwater samples obtained up to 100 m downstream of from swine waste lagoons, indicating that both long-term impact and environmental migration of contaminants occur (19, 20).
Recent studies have attempted to evaluate the impact of antibiotic use on populations of bacteria in natural waters (12, 23, 39). Antibiotic resistance analysis has been used to identify sources of fecal pollution (14, 18, 27, 38, 39). The traditional method involving cultivation and phenotypic testing is still relied upon but has a clear bias when it is used to determine the representative phenotypes and genotypes in this environment. The use of techniques such as PCR and molecular gene probe analysis has allowed sensitive detection of specific genes in the environment in the absence of cultivation. PCR amplification of small-subunit ribosomal DNA (rDNA) genes coupled with denaturing gradient gel electrophoresis (DGGE) has been used primarily to determine the genetic fingerprints of microbial communities. The repertoire of genes used for such analyses has been extended to specific metabolic genes (2, 8, 17, 31, 37) and, recently, to antibiotic resistance genes (1). In the latter work, we used a phylogenetic approach to design a set of primers to target tetracycline resistance genes encoding ribosomal protection proteins (RPPs). These primers were used to detect the corresponding tetracycline resistance genes in ruminal fluid, swine feed and feces, and pig intestinal fecal streptococci. In the present study, we used these techniques to determine the occurrence and migration of RPP tetracycline resistance genes in lagoons and in groundwater underlying two large swine production facilities known to be impacted by waste seepage (19). The use of tetracycline resistance as the key determinant to monitor resistance genes is relevant due to the common use of this antibiotic in the swine industry. To our knowledge, this is the first study in which the genes for one major class of antibiotic resistance were characterized in natural groundwater that is directly impacted by animal agriculture.
Description of the sampling sites.Site A, which began operation in February 1995, is a finishing operation that houses 4,000 pigs (Fig. 1). Antibiotics are used in this facility, but the relevant information concerning such use could not be obtained from the producer. The facility includes a two-stage waste-handling system in which a concrete settling basin collects most of the solids before the supernatant liquid passively enters an earthen lagoon. The area of the lagoon is approximately 1.2 ha, and the lagoon is unlined. No special construction techniques were used to compress the soil during lagoon construction. The average depth of liquid in the lagoon during a study conducted by Krapac et al. (19) was about 1.5 m. The concrete settling basin is periodically pumped, and the manure is applied to crop fields both on-site and off-site.
Site A is located on glacial outwash and terrace deposits along a stream valley that is incised into a till plain formed during the Illinois Episode of glaciation. The topsoils are silt or silty clay loams developed on alluvial deposits that are 1.3 to 2 m thick (Fig. 1). These deposits overlie a 0.6- to 1.3-m-thick upper layer of fluvial silty sand and gravel outwash, which is continuous across the site. Twelve of the 16 monitoring wells were installed in this upper sand layer (Fig. 1). Slug test results suggested that this upper sand has a saturated hydraulic conductivity of approximately 6.8 × 10−4 m/s. Below the silty sand and gravel is 1.6 to 3 m of silt loam diamicton, which may be colluvial. Below the silt loam diamicton is a 1- to 2-m-thick lower sand layer composed of sand and gravel outwash that is being used locally as an aquifer. Four monitoring wells were installed in this lower sand layer (Fig. 1). The saturated hydraulic conductivity of this deeper sand was estimated to be 8.2 × 10−6 m/s based on slug tests. Below this sand and gravel is more silt loam diamicton. Logs from water wells drilled in the vicinity show the presence of discontinuous sand and gravel outwash units below the diamicton that are used locally as aquifers. The multiple sand layers make this site particularly susceptible to leachate migration from the lagoon.
Site C is a farrowing and nursery operation that houses 1,200 sows (Fig. 1). The facility uses chlortetracycline, which is added once per quarter for about 2 weeks to the feed (400 g per ton). The feed consumption rate is about 3 kg/animal per day, and thus, each animal consumes about 17 g of chlortetracycline over the 2-week period. The facility began operation in the fall of 1992 and every year produces approximately 24,000 pigs. The facility uses a single-stage lagoon. Lagoon water is recycled to partially fill and flush the shallow pits below the confinement buildings. The area of the lagoon is approximately 0.8 ha, and the lagoon is unlined. The average depth of waste in the lagoon was about 6 m. Waste has never been applied to the crop fields surrounding the lagoon.
Site C is located on a glacial till plain formed during the Illinois Episode of glaciation. It is underlain by a silt loam glacial diamicton that is 3 to 15 m thick and overlies shale bedrock (Fig. 1). Thin (less than 30-cm-thick) glacial gravelly loam layers were found in two of the seven borings at the site. An intermittent stream borders the site on the west, and a small pond is located about 100 m south of the lagoon. Large-diameter bored and dug wells and ponds are the predominant sources of drinking water in the area.
Groundwater and lagoon sampling.Groundwater was collected from 14 monitoring wells at site A and from six monitoring wells at site C (Fig. 1). Water samples from the waste lagoons were also taken from each site. A polyethylene bailer dedicated to each sample was sterilized with alcohol and rinsed with sterile water prior to use for sample collection. Between 1.5 and 3 well volumes of groundwater was removed from each well before collection as described previously (19). Samples were stored in clean, sterile plastic bottles and kept on ice in the field. Samples were refrigerated at 4°C in the laboratory until they were analyzed.
Bacterial identification and isolation.Tetracycline-resistant bacteria from lagoon and groundwater samples were grown aerobically on Enterococcosel agar (BBL, Cockeysville, Md.) and MR2A agar (9); 20 μg of tetracycline (Sigma, St. Louis, Mo.) per ml was added to each medium. Undiluted groundwater samples (100 μl) were directly plated onto agar media, while lagoon samples were plated by using 10-fold serial dilutions up to 1,000-fold. Media lacking tetracycline were inoculated similarly. Cultures grown on Enterococcosel agar were incubated at 37°C for 48 h. Cultures grown on MR2A agar were incubated at room temperature for up to 14 days. Tetracycline-resistant colonies were further purified by restreaking on the same media and were used for PCR screening as described previously (1). Gram staining was performed with a Protocol kit (Biochemical Sciences, Swedesboro, N.J.).
DNA extraction.Groundwater samples (250 ml) were centrifuged at 17,700 × g for 20 min at 4°C. The supernatants were discarded, and the pellets were washed three times with 0.1 volume of phosphate-buffered saline (120 mM NaH2PO4 [pH 8.0], 0.85% NaCl) before extraction of total DNA by the method of Tsai and Olsen (36). Lagoon samples (50 ml) were centrifuged at 10,000 × g for 10 min at 4°C before DNA extraction as described above. DNA (final concentration, 125 ng/μl) was stored in TE buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8.0]) at −20°C.
PCR amplification.The primers and PCR protocol used to amplify tetracycline resistance genes in this study were described previously (1). Briefly, PCR was performed with 25 pmol of each primer and an ExTaq PCR kit (purchased from PanVera Corporation, Madison, Wis.) by using a final reaction mixture volume of 25 μl. Purified DNA (125 ng) or one-half of a 1- to 2-mm-diameter individual colony that was resuspended in sterile water was used as the PCR template. PCR amplification was performed with a GeneAmp PCR system 2400 thermocycler (Perkin-Elmer, Norwalk, Conn.). The temperature program consisted of denaturation at 94°C for 5 min, followed by 30 cycles consisting of 94°C for 30 s, annealing for 30 s, and extension at 72°C for 30 s and a final extension at 72°C for 7 min. The annealing temperatures used for amplification of different RPP genes were as follows: tet(O), 60°C; tet(Q), 63°C; tet(W), 64°C; tet(M), 55°C;tetB(P), 47°C; tet(S), 50°C; otrA, 66°C; and tet(T), 46°C. The PCR protocol was modified for DNA obtained from groundwater samples due to the presence of unidentified PCR-inhibiting substances. For these samples, a second, nested PCR was performed by using 1 μl of the first PCR mixture as the template and amplifying the template for 25 or 30 cycles as described above. Nested PCR was also performed with DNA from lagoon samples when necessary. The control reactions included PCR amplification with bacterial 16S rDNA primers 27F and 1525R (21) and sterile water or DNA of tetracycline-sensitiveEscherichia coli EPEC as the negative control template for all primer sets and the positive control strains for each primer set as described previously (1). PCR product aliquots (5 μl) were analyzed by electrophoresis on a 2.5% (wt/vol) agarose gel (NuSieve; FMC Bioproducts, Rockland, Maine) and were stained with ethidium bromide. The sizes of the PCR products obtained from amplification were 167 bp for tet(O), tet(Q),tet(W), tet(M), tetB(P),tet(S), and tet(T) and 212 bp forotrA.
DGGE analysis.DNA from groundwater, lagoons, and bacterial isolates was PCR amplified as described above, except that in the nested PCR the forward primer included a GC clamp at the 5′ end. Primers that targeted tetracycline resistance genes and the V3 variable region of 16S rDNA (25) were used. DGGE analyses of the amplified tetracycline resistance genes were performed as described previously (1). Briefly, electrophoretic separation of the PCR products was accomplished by using a polyacrylamide gel with a gradient containing urea and formamide (8% acrylamide, 30 to 60% urea–formamide, 0.5× TAE buffer [pH 7.4]). Electrophoresis was performed at 60°C for 2 h at 150 V and then for 1 h at 200 V, using a D-Code system (Bio-Rad, Hercules, Calif.). The gels were silver stained, and images were captured by using the Bio-Rad Diversity Database fingerprinting software on a G3 Macintosh computer equipped with a Bio-Rad GS-710 calibrated imaging densitometer. The gel standard markers consisted of known mixtures of PCR-amplified RPP tetracycline resistance genes or the V3 variable region of 16S rDNA.
Cloning and sequencing.DNA bands were excised from DGGE gels, crushed, equilibrated in 50 μl of sterile water, and then subjected to three cycles of freezing and thawing (at −20°C and room temperature). Eluted DNA (1 μl) was reamplified with the corresponding primers and reelectrophoresed on a DGGE gel as described above. PCR products were cloned by using a TOPO-TA Cloning kit (Invitrogen, Carlsbad, Calif.) according to the manufacturer's instructions. White colonies harboring the corresponding tetfragments were identified by PCR by using the small amounts of colony biomass and the corresponding primer sets. Representative bacterial isolates from the groundwater and lagoons were identified by sequencing of the cloned 16S rRNA genes, which were amplified with the bacterial primer set (21). Sequence analysis of the clonedtet and small-subunit rDNA fragments was performed by the University of Illinois Keck Center for Functional and Comparative Genomics. Sequences were analyzed on-line by using the BLAST (Basic Local Alignment Search Tool) family of programs of GenBank (22).
PCR amplification of tet genes from lagoon and groundwater samples.All of the known ribosomal protection tetracycline resistance determinants were detected in the lagoon samples (Table 1). Most of them were detected in total DNA from the lagoons after the initial 30 cycles of PCR; the only exception was otrA, which was detected only after a second, nested PCR. The most frequently detected determinants in groundwater samples from both sites were Tet Q, Tet W, Tet M, Tet T, and Tet O. Groundwater from well A8 contained Tet S, and well C6 water contained detectable otrA. None of the groundwater samples contained detectable tetB(P). Background well A7 at site A contained tet(T), which was also detected in more than one-half of the wells located downstream of the site lagoon in the direction of groundwater flow. Site C background well C1 did not contain any of the ribosomal protection tetracycline resistance determinants. Two of the shallow wells at site A, wells A10 and A4, also did not contain any detectable levels of these determinants. Well C3, located close to the lagoon but perpendicular to the general direction of groundwater flow away from the lagoon, did not contain any determinant. Sample A14 from the well located farthest away from the site A lagoon contained Tet Q, which was the most frequently detected determinant.
One of the objectives of this study was to determine whether the genetic diversity of tetracycline resistance genes uncovered by in vitro analysis of total DNA overlapped the genetic diversity of the cultivable isolates. Also, amplification of tet genes from total-DNA preparations is not discriminatory in terms of the host strains in which tet genes occur, and our aim was to determine whether tet gene dissemination to groundwater and indigenous soil microbiota could occur. The experiments which we performed are described below.
Phenotypic characterization and tetracycline resistance genotypes of bacterial isolates.Approximately 4 × 103 and 3 × 104 CFU per ml were obtained on Enterococcosel agar containing 20 μg of tetracycline per ml after direct plating of 100 μl of slurry from the lagoons at sites A and C, respectively. Several presumptive enterococci (gram-positive, catalase-negative cocci) were randomly selected and isolated (Table2). All of the enterococcal isolates contained tet(M) as determined by PCR, and one isolate (CLE3) also carried tet(S). Another randomly selected isolate obtained from the Enterococcosel medium was a gram-positive rod (CLE4) which also harbored the tet(M) gene. Few tetracycline-resistant colonies were obtained after direct plating of groundwater on Enterococcosel agar.
Cultivation of aerobic heterotrophs from undiluted groundwater on MR2A medium yielded counts between approximately 1.3 × 103and >105 CFU per ml. In the presence of 20 μg of tetracycline per ml, only groundwater from well A8 (1.7 × 102 CFU per ml) and the lagoons (>105 CFU per ml) yielded significant numbers of tetracycline-resistant colonies. Tetracycline-resistant colonies were obtained sporadically from direct plating of groundwater from several wells (wells A7, A3, A16, C2, and C7). These colonies, along with several randomly selected tetracycline-resistant colonies from well A8 and lagoon samples, were isolated. All of the MR2A medium isolates from groundwater and lagoon samples were distinct gram-negative rods. Of the 16 tetracycline-resistant isolates, 10 contained the tet(M) gene. The remaining six resistant isolates did not harbor any of the ribosomal protection tetracycline resistance determinants and presumably harbored the efflux mechanism of resistance.
DGGE analysis.DGGE analysis of the tet(M) determinant was used to assess conservation of the gene in both environmental samples and individual isolates. One major band corresponding to tet(M) was present in all of the groundwater and lagoon samples (Fig. 2). Multiple minor bands were present in several samples (e.g., well C7); however, when these bands were extracted from the gel, reamplified, and reelectrophoresed on a DGGE gel, the migration was identical to the migration of the major band and the positive control fortet(M) (data not shown). DGGE analysis for thetet(M) gene in isolates also demonstrated that there was one principal band corresponding to the tet(M) control band. The isolates were also analyzed by DGGE by using primers specific for the V3 region of the 16S rDNA (Fig. 3). The results indicated that isolates harboring the same tet(M) gene were representatives of at least 10 different phylotypes at sites A and C.
Identification of tetracycline-resistant bacteria.Several tetracycline-resistant isolates were selected for further identification by 16S rDNA sequencing. The strains that were selected on Enterococcosel medium belonged to the low-G+C-content gram-positive bacterial phylum, and the closest relatives wereEnterococcus sp., Staphylococcus sp., andLactobacillus reuteri (Table3). Growth on MR2A medium resulted in a wider range of taxonomic affiliations, including members of the alpha and gamma subdivisions of the class Proteobacteria and theActinobacteridae subclass of high-G+C-content gram-positive bacteria (Table 3). The only tetracycline-resistant isolate obtained from the background well at site A was identified as a member of the genus Bosea, which includes bacteria mostly of soil origin. With the exception of L. reuteri, all of the isolates had levels of similarity with database entries between 91 and 96%, which allowed only approximate taxonomic identification at the genus level and higher taxa levels.
Tetracycline is commonly used in animal agriculture, particularly in the swine industry (16). Because of this use, selection for tetracycline resistance occurs in the normal swine gut microflora, and in our previous study we demonstrated that the reservoir of tetracycline resistance determinants in the swine intestinal microbiota is substantial and diverse (1). However, the details of what happens with this pool of antibiotic resistance genes further downstream remain largely unknown. Since animal production systems are not closed ecosystems, this pool may be released into the environment. In this study, we demonstrated that a broad range of tetracycline resistance genes occur in two swine waste lagoons and that upon release into the environment these genes can potentially mobilize and persist.
Detection of all of the RPP genes in the waste lagoons at both sites clearly indicated that selection for this drug resistance trait occurs and that a number of resistance genes can be maintained in the microbial populations present. A wide range of RPP genes were detected in groundwater downstream of the waste lagoons. Greater occurrence of RPP genes was detected in wells proximal to both lagoons in the direction of groundwater flow, and the detection of tet(Q) in well A14, located more than 250 m downstream of the lagoon at site A (Fig. 1), suggested that the mobility of resistance genes in the environment can be substantial. Two of the locations at site A had nested wells (Fig. 1, wells A4 and A6), and second wells were screened in deeper sand layers (wells A3 and A5). The occurrence of RPP tetracycline resistance genes was greater in the deep wells than in the corresponding shallow wells, demonstrating that contaminants may be vertically mobile depending on the hydrogeology of the location. Although this study was based on a single sampling event, the data suggest that the presence of the tetracycline resistance genes is due to seepage and movement of groundwater underlying the lagoons. Wells A10 and C3 did not contain any of the tetracycline resistance determinants, as expected due to the locations of the wells relative to the lagoons and the direction of groundwater flow. Thus, there were clear gradients of relative frequency and diversity of tetracycline resistance genes, with the maximal values occurring at waste lagoons and a gradual decline in the direction of groundwater flow; however, the genes were still detectable 250 m downstream. These observations may have important implications for understanding the circulation and acquisition of antibiotic resistance genes. Groundwater constitutes a substantial part of the public water supply in the United States (http://water.usgs.gov/wid/html/GW.html ), and at both study sites it is used as the predominant source of untreated drinking water. Thus, along with other ways of acquiring antibiotic resistance, such as consumption of tainted food, the occurrence of antibiotic resistance genes in drinking water provides a possible way for antibiotic resistance to enter the animal and human food chain.
Several tet genes, particularly tet(M),tet(O), tet(Q), and tet(W), were dominant in the total communities in the lagoons and groundwater. These genes were found to be predominant in the gastrointestinal tracts of pigs and steers (1), and in this study the elevated frequencies of these genes in the environment surrounding the farms were consistent with the hypothesis that this occurrence was the result of gene flow from the animals. Tetracycline-resistant bacteria obtained from the lagoon and groundwater samples primarily harbored thetet(M) gene, probably because the cultivation technique was biased towards aerobic growth and the group of bacteria containing the known tet(M) genes includes members of a broad range of genera, including genera of gastrointestinal origin (Fig.4). Furthermore, it is known that alleles of tet(M) are present in a broad range of bacteria, and their transfer is presumably mediated by conjugative transposons Tn916, Tn1545, and Tn5251 and large conjugative plasmids. It is notable, however, that the use of two different media and random selection of tetracycline-resistant colonies resulted primarily (75%) in bacteria harboring tet(M). No groundwater isolates were correlated with a high occurrence of Tet T and Tet O, the two determinants which occur in aerotolerantStreptococcus spp. (Fig. 4). None of the other RPP tetracycline resistance genes were detected in any of thetet(M)-harboring isolates except CLE3, which contained bothtet(M) and tet(S). In some tetracycline-resistant isolates (A7-2, AL-1, AL-5, CL-2, A3-1, and C7-1) no genes conferring ribosomal protection were detected, and these isolates most likely have an alternative mechanism of tetracycline resistance, such as energy-dependent efflux of tetracycline. Anaerobic genera such asPrevotella, Bacteroides, and Butyrivibrio were not targeted for cultivation in our study, and consequently, isolates containing the two most prominent lagoon and groundwater markers [tet(Q) and tet(W)] were not represented. Few groundwater samples contained tet(S), otrA, andtetB(P); however, these determinants were detected in the lagoons, which suggested that they were present at significantly lower levels than the other RPP tetracycline resistance genes.
Enterococci were the dominant fecal bacteria detected in lagoons and in several groundwater samples. Tetracycline-resistantEnterococcus spp. were isolated along with an organism known to be of gastrointestinal origin, L. reuteri. The presence of identical tet(M) alleles in these bacteria suggests that lateral transfer of the gene occurred; however, it cannot be determined if this event occurred in the gut of an animal, in the lagoon environment, or in the groundwater. This gene and tet(O) were found to be circulating among the fecal streptococci in the pig gut (1). In this work, more significantly, the potential for transfer of the tet(M) allele involving groundwater and commensal soil bacteria was evident. Taxonomic analysis of isolates carrying the same tet(M) gene and belonging to the generaAfipia and Microbacterium confirmed that they probably originated from soil. Very little is known about the occurrence of tet(M) and other tetracycline resistance genes in phenotypically tetracycline-resistant bacteria in soil. Most of thetet(M) genotyping work has been done with clinical isolates, and interestingly, the genes are genetically quite diverse (92 to 96.8% identity) and display a mosaic structure consistent with homologous recombination between different lineages (3, 10, 15, 26). In our experiments, we observed extreme homogeneity of these genes in taxonomically (and ecologically) diverse bacteria, including intestinal lactobacilli, enterococci, and staphylococci, as well as soil species of Microbacterium and Afipia(Fig. 4). Consistent with this observation, we hypothesize that in our case we found a point source of genetic contamination originating on farms with the selective pressure of tetracycline. It is likely that transfer events occur in part because of the high density of bacteria in the lagoons, but it is not known to what extent such events occur in less populated groundwater environments. The observed horizontal spread of tet(M) genes to soil bacteria may have several notable implications. First, such spread suggests that antibiotic resistance gene dissemination is not restricted to the release of antibiotic-resistant gastrointestinal microbiota, which may have limited viability outside the gastrointestinal tract. The dissemination vector is not interrupted even if the original antibiotic resistance-harboring bacteria are not viable anymore. Second, when the resistance gene pool is mobilized into the indigenous bacteria in the environment, it has a much better chance of survival, persistence, and mobility, effectively increasing the gene frequency in local populations and having increased potential for reaching other ecosystems.
Another explanation for the elevated occurrence of tet(M) in soil bacteria may be the selective pressure of tetracycline, which may move through the soil layers to the groundwater and select for tetracycline resistance genes residing in soil microbiota. Few previous studies have addressed the fate of antibiotics in the environment and, if they are present, whether they exert any selective pressure for resistance. Tetracycline is not known to be readily degradable and is reportedly strongly absorbed in various soil types (28). This, along with the constant input of tetracycline via animal waste, can potentially provide a concentrated environment in which selection can occur. However, because of the strong sorption of tetracycline in soil (28), this antibiotic may have limited ability to reach distant groundwater layers and is concentrated primarily in soil layers close to lagoons. Despite the possibility of accumulation of tetracycline in soil environments, even low levels can substantially increase the transfer frequency of large conjugative transposons, such as Tn916 (32) and Tn1545(5), Tn916-like elements (29, 35), or Bacteroides transposons (30) on which tetracycline resistance genes reside. Other factors conducive to resistance persistence and acquisition in the environment are not known, and groundwater is a unique environment for study. In particular, these factors could be naturally occurring compounds with structural similarity to tetracyclines, such as certain plant flavonoids.
In this study, we estimated the pool, diversity, and possible transmission of one group of tetracycline resistance genes in two animal production systems and their surrounding environments. Although previous studies have addressed the occurrence of specific antibiotic resistance characteristics in the environment, none of these studies included a more thorough genotypic characterization of a specific antibiotic resistance trait. While the set of genes which we targeted in our study includes only a limited portion of the bacterial resistance genes that may be present, it provides the basis for using a similar approach in order to combine molecularly and phenotypically based methods to characterize the occurrence, diversity, and routes of transmission of antibiotic genes in nature. The data obtained can be used to address the question of whether environments such as groundwater and soil are significant reservoirs for dissemination of resistance genes. In addition, long-term surveillance can provide an indication of the mobility, persistence, and transfer of resistance genes and begin to address the issue of agriculture as a source of resistance genes for entry into the environment.
- Received 30 November 2000.
- Accepted 9 January 2001.
- Copyright © 2001 American Society for Microbiology


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ICDSP 2020: 2020 4th International Conference on Digital Signal Processing
Chengdu, China, June 19-21, 2020
|Conference website
|Submission link
|Submission deadline
|April 20, 2020
2020 4th International Conference on Digital Signal Processing (ICDSP 2020) will be held in Chengdu, China during June 19-21, 2020. It is an annual research conference which is mainly organised by International Academy of Computing Technology (IACT). The past 3 ICDSP conferences were held in Kuala Lumpur, Tokyo, Jeju Island respectively.
Submission Guidelines
Full Paper(publication and oral presentation)
Abstract(oral presentation only)
Electronic Submission System (.pdf)
List of Topics
Adaptive Filtering & Signal Processing
Ad-Hoc and Sensor Networks
Analog and Mixed Signal Processing
Biometrics & Authentification
Biosignal Processing & Understanding
Communication and Broadband Networks
Communication Signal processing
Radar Signal Processing
Rapid Prototyping and Tools for DSP Design
RF and Wireless Communications
Sensor Array and Multi-channel Processing
Signal Processing and Communications Education
Statistical Signal Processing
TF Spectrum Analysis & Wavelet
Time-Frequency/Time-Scale Analysis
Video compression & Streaming
Watermarking and Information Hiding
Conference Advisory Chair
Prof. Erchin Serpedin, Texas A&M University, USA
Conference General Chair
Prof. Franklin Bien, Ulsan National Institute of Science and Technology, Korea
Prof. Yow Kin Choong, University of Regina, Saskatchewan, Canada
Conference Technical Program Committee Chairs
Prof. Isao Nakanishi, Tottori University, Japan
Prof. Yasuhiro Matsuda, Kanagawa Institute of Technology, Japan
Prof. Ir Dr Goi Bok Min, Universiti Tunku Abdul Rahman, Malaysia
Invited Speakers
Prof. Erchin Serpedin, IEEE fellow, Texas A&M University, USA
Prof. Junchul Chun, Kyonggi University, Korea
Prof. Franklin Bien, Ulsan National Institute of Science and Technology, Korea
Prof. Yasuhiro Matsuda, Kanagawa Institute of Technology, Japan
Professor Ir Dr Goi Bok Min, Universiti Tunku Abdul Rahman, Malaysia
All papers will be published in the International Conference Proceedings Series by ACM(ISBN: 978-1-4503-7687-7), which will be archived in the ACM Digital Library, and indexed by Ei Compendex and Scopus and submitted to be reviewed by Thomson Reuters Conference Proceedings Citation Index (ISI Web of Science).
Gretchen Liu
E-mail: email@example.com
Tel: +86-18081079313


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SINGAPORE - Eight local and international sailing teams will compete in this week's DBS Marina Bay Cup, the largest match racing competition on the local calendar.
Into its second year, this year's edition will feature twice as many teams and include participants from Australia, Malaysia, Thailand, the Philippines and host Singapore during the Sept 15-18 event.
Team Philippines, led by 2015 SEA Games keelboat match racing champion Ridgely Balladares, are the defending champions but face stiff competition from Team Sime Darby Foundation, skippered by Malaysia's top sailor Jeremy Koo, ranked 17th in the world.
Other teams in action at the Marina Bay include those from the Singapore Management University and University of Western Australia.
Practice sessions are on Thursday followed by three days of match racing.


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This week we began our Spring study with an introduction to our topic. We will be studying Romans chapter 8, using Trillia Newbell's If God Is For Us: The Everlasting Truth Of Our Great Salvation.
But Why Romans, and why only chapter 8? I turned to James Montgomery Boice to help us understand why, out of all the Bible, we should choose one chapter from one book. He opens his commentary series on Romans with the following: Continue reading "Romans 8, An Introduction"


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NEW ORLEANS, LOUISIANA--Every Friday, we team up with the Historic New Orleans Collection to bring you something unique from our city's past. For today's find, you need a date! We are headed to a high school dance for the class of 1964.
It's not news that Irma Thomas is awesome. She is the soul queen at the heart of New Orleans.
Eric Seiferth, the Associate Curator Historian at the Historic New Orleans Collection shares some publicity photographs of Ms. Thomas from the mid 60's in the beginning of her career. Among the photos was a contract for a performance at the Jung Hotel, playing with Sugar Boy Crawford's band the King Cutters.
The occasion was a high school dance.
The price for the performance was one hundred and fifty dollars minus a 25 five dollar commissions charge.
Seiferth says "Benny Spellman was someone she performed with frequently and they would alternate sets so according to this contract she is to perform from nine to midnight alternating with Spellman with was pretty usual. They were all over town performing."
Irma Thomas was once a waitress at the Pimlico Club.
She seized the sound waves of the 60's with a series of popular hits.
During this time, artists would perform local gigs frequently to help promote themselves as well as earn some cash.
Thomas' song Breakaway, was a big hit during this time. It's the song about a romance you can't get seem to getaway away from.
Seiferth says, "that was a big local hit and I'm sure the teenagers at the dance were excited to hear and dance to it."
Each new generation enjoys the technological advances originating from the past generations. However, THIS AUTHOR prefers the high school dances of old! These days, teenagers get a dj at the prom.
The the young men and women at the St. Aloysius dance in 1964 received the memory of a performing queen in her prime.
"She's an amazing artist and an amazing part of New Orleans' rich rock and roll and blues history," says Eric Seiferth.
Make sure you tune in every Friday to see what we find out of the Historic New Orleans Collection.


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Which writers or poets inspire you and why?
I have been inspired by many and varied writers and poets, too many to name. As a child I loved Enid Blyton and grew into R.F Delderfield and progressed to Jane Austen. My recent reading material has included George Martin and Dorothy Dunnett, together with a variety of modern writers.
If you are a writer or poet, how did you get started?
I started writing stories as a child under the age of 10. Intially I made tiny comics for my sister and friends. As I grew up I wanted to be a journalist. I did achieve this ambition but soon decided it was not the kind of writing I wished to do. After many years working and looking after the family I turned my hand to short story writing and now I am writing my first novel.
Where and when do you do most of your reading?
I don't find I have the time to read as much as I'd like or feel I should. I grab any free moments and occasionally read at bedtime. Most reading, these days, is done when I'm on holiday.
Are there some themes you enjoy more than others?
I love reading history, both fiction and non-fiction. I also enjoy biographies, particularly of women in history.
What are you reading currently?
The Kashmir Shawl by Rosie Thomas.
Are you a library lover, a bookshop bird or an online owl?
Where there are books, there am I! I love libraries, I will spend hours trawling a book shop and I have a huge 'to be read' collection on my kindle.
Which genre of writing do you prefer and why?
I love reading history, both fiction and non-fiction. I also enjoy biographies, particularly of women in history. I also enjoy some fantasy novel. I think it's because I like to escape!


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Thu, 12 Jul 2018
What is Prior's Court?
Prior's Court offers education and residential care for young people aged five to twenty-five whose autism needs mean they require a lifetime of support. For these young people life can be extremely challenging. But with the right support, amazing things can be achieved.
Set on a beautiful 50 acre site in West Berkshire, we have extensive facilities adapted to support the complex needs of young people with severe autism.
We believe in supporting each young person according to their specific needs so we ensure staff working with each young person gets to know them well and builds a trusting and healthy relationship.
There are over 500 staff at Prior's Court working full time and part time to ensure that there is 24 hour support throughout 52 weeks of the year. Staff work in various roles within the school and young adult provision including therapists, teachers in the school and learning facilitators in the young adult learning centre, home managers, autism practitioners / support workers (education, residential and night teams), medical staff and family support. Staff also work in the departments which support them including IT, administration, Human Resources and training, finance, admissions, marketing and fundraising, housekeeping and estates maintenance.
We are looking for outstanding people to join our outstanding provision and we currently have the following vacancies:
Full/Part time and weekend Autism Practitioner
Shift times 7am -3.45pm and 3.15pm – 10pm.
Salary FTE £17,800 - £23,000 depending
on Qualifications, skills and experience
3 Nights per week Autism Practitioner
Week 1: Wednesday/Thursday and
Sunday 9.45pm – 07.15am
Week 2: Thursday/Friday and
Saturday 9.45pm – 07.15am
Salary £13,500 - £17,250 depending on Qualifications, skills and experience
Monday – Friday 7am – 2.30pm
Starting salary £15,015
35 hours per week
Instructor for Vocational and lifelong Learning
Monday – Friday 8.30am – 4.30pm
Salary £20,445 - £32,000 depending on Qualification, skills and experience
If you are interested in any of the above vacancies please call our Recruitment team on 01635 245928
or email: firstname.lastname@example.com for more details.
We would encourage you to come along at an agreed time and visit our amazing organisation to find out more about working at Prior's Court.
Registered Charity No: 1070227


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App Camp for Girls 2017 — App Camp For Girls, a non-profit organization aimed at correcting the gender imbalance in the software industry, has opened 2017 registration for its summer program that teaches girls in the US how to make mobile apps in one week.
Camp tuition is US$400 for the week. In addition to launching a new location in Chicago, the program will again take place in Portland, Seattle, and Phoenix. Parents can register for 2017 sessions on the App Camp For Girls website. All sessions will be held in July. Space is limited; early registration is recommended.
What are we doing over here in New Zealand? Apart from this worthy effort, of course. And things like Code@MOTAT, which is open to all kids 8 and up with their own laptops. The national organisation site is here.
WikiLeaks document dump reveals CIA iPhone penetration team, insecurity of exploit library — A new batch of 8,761 files was released on Tuesday by WikiLeaks that alleges that the US Central Intelligence Agency has a dedicated iOS exploit team —and lacks the ability to keep the attack vectors under lock and key.
Adobe updates Lightroom pro photo apps for iOS with 'Authentic HDR' & more — Adobe has released updates to the iPhone and iPad versions of Lightroom, making various enhancements including the addition of 'Authentic HDR', a new shooting mode.


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When I first found out I was going to Dunedin, I remember thinking "Where's this place? Is it true there are more sheep than people? How on earth am I going to survive knowing that shops close at 5? What can I do for fun?"
I'm sure these questions are pretty drastic but for a girl who found out she was going to Dunedin to study in less than a month… well, it was pretty understandable.
I was an interesting case because my parents were the ones that applied to the foundation school in New Zealand for me.
They wanted me to have an overseas education and pretty much uprooted me from my school in Singapore to attend a pre-university course in NZ. And I am so grateful for that every day.
Living on my own in a foreign country can be scary, especially if you don't know anything about the city (well except from Wikipedia, of course). All I knew about Dunedin was that it was a university town and that there was probably nothing much to do after 5 pm except to stay at home and chill.
Thus, my first few weeks in Dunedin mainly consisted of me going to classes and going home. It does sound boring, doesn't it? Well, the only way from the bottom is up and things started changing when I met my best friend Caroline.
Caroline and I didn't have a good impression of each other when we first met. I thought she was too cool for me, and she thought I was way too quiet. We couldn't have been more wrong about each other!
Anyway, after a rocky start, we gradually became really close and she got me to join the Otago Singapore Club (OSC) as she was the president in 2010-11.
I really admired the work she had done because she was organising all these food events, paintball events, etc. to bring us Singaporeans together. It was through her that I was able to open myself up to people and when I started to truly feel like a part of the Dunedin community.
I know some people may not agree with me on hanging out with my fellow countrymen, because who goes to a different country and hang out with the same people you hang out with back home?
But in truth, joining an activity (be it your cultural club, a sport, a musical activity) is the best place to make friends if you're someone like me.
Otago University has a whole slew of clubs that have the loveliest people you will ever meet and will get you immersed in the university culture right away (http://www.ousa.org.nz/recreation/clubs/).
From archery, kickboxing, to dance, Otago has it all for the shy and quiet individual.
Even if you're a naturally brave and outgoing person, joining an activity won't hurt as you will be making new friends and learning a new skill at the same time!
Anyway, while I took the comfortable route of making friends with people from similar backgrounds, it was a great platform for me to meet people of all cultures.
Dunedin is such a small and close-knit community that it is impossible to not know anybody who is from another country.
We have so many inter-club activities that also help you meet people all over the world!
I used to joke that my friends are similar to the United Nations because my friends are from New Zealand, Hong Kong, the Netherlands, Malaysia, India and more.
Furthermore, one of the best things about Dunedin is the annual International Food Festival whereby the entire international clubs band together to cook up a feast for the public.
You can afford cheap and delicious food from the different cultures, and groove to the sounds of a live band. Throughout my years at Otago, the International Food Festival just keeps getting bigger and better! And it is the only international food festival in the whole of the south island.
Joining an activity/club will also give you a break from all that study.
Instead of lying in bed at 8 pm and feeling horrible about your latest assignment, you could be going out for concerts or dancing with your friends!
I don't know about you, but I get bored easily so I always jumped at the chance of planning exciting events for my fellow international friends.
Being the president of the International Cultural Council (ICC) has expanded my repertoire outside of the academic sphere and has given me opportunities to meet people from all walks of life.
One of my greatest accomplishments this year was organising the International Cultural Night – which is basically a concert showcasing all the different cultural performances to the wider Dunedin community.
While stressful, I have never had so much fun before. It was great to be in the midst of all the action and seeing all the hard work come alive on that one night. Moreover, all proceeds went towards charity and it was nice to know that everything paid off for the better
So let me just say this: I never expected to be where I am right now. It goes to say that life is unexpected and you just have to go with the flow sometimes. But I have no regrets because being the ICC President gave me the chance to escape from those long dreary hours of study, and helped me to be a more self-assured and confident individual.
I have also met so many great people who will always remain close to my heart. So what are you waiting for? Go out, explore, and make new friends. You will be surprised to know that we don't bite.
Picture Credits to Otago Singapore Club and Hadi


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California Measles Outbreak Is Over
The happiest place on earth is finally safe again. The Disneyland-based measles outbreak that began in California in December is over, said public health officials on Friday. According to CDC reports, the outbreak was the largest since the 1989 epidemic which killed 70 people, and was likely brought into the country by a foreign traveler or an American returning from an overseas trip. Because of the high numbers of visitors the Disney park attracts every day, it's not surprising that the virus spread quickly. But as of Friday, said the Department of Public Health, no new cases had been reported in over 42 days. Despite the momentary quell in reported cases, officials say that pending legislation that would require all children to be vaccinated prior to entering kindergarten — and would likely prevent another epidemic like the one that originated at Disneyland — isn't going away anytime soon.
Luckily, out of the outbreak's 147 reported cases — which spanned seven U.S. states, Mexico, and Canada — no deaths were reported. But that doesn't mean it's back to the old grind.
"This is a wake-up call to make sure that we keep measles from regaining a foothold in our country," said Dr. Anne Schuchat of the CDC in a statement to the press on Jan. 29, just as the outbreak began gaining major traction. "This [outbreak] is not a problem of the measles vaccine not working, it's a problem of the measles vaccine not being used."
In order to stave off another wave of measles, several California legislators are considering bills which would essentially protect the public against a similar epidemic by forcing parents to obtain the proper vaccinations for their children.
"We are clearly at a point where our community immunity is dropping too low," said California State Senator Richard Pan, a physician, in a State Health Committee meeting on Wednesday. "[To believe otherwise] is a luxury."
A Senate Education Committee vote on measure SB 277, which would have required all children to be fully vaccinated, was stalled by a week on Wednesday after protesters swarmed the state capitol to contest what they believed was a scientifically unfounded piece of legislation.
"I strongly oppose injection of questionable materials into the bodies of our children as a condition of education," said environmental engineer Steve Wall in a comment to The New York Times. According to The Times, if the measure had passed, it would have made California the largest state to ban "exemptions from vaccines for any reason other than medical necessity", along with only two other states — Mississippi and West Virginia.
The outrage over the measure took some of the senators and supporters by surprise. "This [anti-vaccine movement] is a movement that has taken off, and you have people who feel so deeply about it that it doesn't matter what information they get," said J. Theodore Anagnoson, professor emeritus of political science at California State University, in an interview with The Sacramento Bee on Thursday.
Since Friday's announcement that the measles outbreak had ended, however, many are choosing to focus on the positive while they await the Committee's upcoming vote.
"They did a tremendous job," said Dr. James Cherry, a UCLA infectious disease specialist, in an interview with The Washington Post, speaking of the health workers who stemmed the epidemic before it could get worse.
However, The San Francisco Chronicle reported that health officials were warning members of the public not to rest on their laurels. With the anti-vaccination movement growing to a clamor, they said, it was likely that health workers would see an influx in further viral outbreaks over the coming years unless those legislative guidelines were passed.
"Measles can be reintroduced in California at any time when an infected person brings it to the state," said Dr. Karen Smith, director of the California Department of Public Health, in a statement on Friday morning. "The best defense for protection against the highly infectious measles is vaccination."
Images: Getty Images (1)


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VIRTUAL NJ Walks for TS
To meet the needs of our on-the-go families and to ensure the greatest impact, NJCTS expanded NJ Walks for TS to encompass the entire state throughout the spring.
The NJ Walks for TS Virtual Walk will allow your family and friends to show support wherever you choose anytime in March, April or May. And be sure to wear your t-shirts and send us your pictures so we can spread the Walk message on our social media channels. We will also share those pictures with the Governor, Commissioner of Education, Commissioner of Health and others as part of TS Awareness Day in June to introduce them to the faces of the 1 in 100 kids and families living with Tourette Syndrome across our state.
Register as a Walk Team Captain << Click on this link and choose CREATE MY OWN FUNDRAISING PAGE. Registered captains will receive a 2020 NJ Walks for TS t-shirt when they donate or fundraise $25. Recruit your friends and family to join your walk (or whatever activity you choose) and ask them to support your walk by making a donation. Teammates who wish to purchase a shirt, may do so by using the link or form below. Their $25 will be added to your final fundraising total. Register by March 1 to get your team name on the back of the shirt!!
Once you have registered your team, you will have your very own fundraising page! You can update your page with your story and photo. Then send the link to family and friends asking for their support.
Make a Donation to the Event or a Participant << Looking to make a donation to NJCTS or on a participant's behalf? Click on the link above and then on "Top Fundraisers." If you can't find their name, make a general donation and let us know which participant to credit.
Purchase a NJ Walks for NJ T-Shirt << Your purchase will support the event. Please indicate on the form (either online or printed) which team you would like credited with the purchase.
Sponsor the Event << The deadline to become a sponsor and make it on our t-shirt is February 28. Follow the link for other sponsor benefits. (Download printable form.)
Make sure you wear your shirt when you do your walk, run or ride and have someone snap a photo of you with your teammates, family and friends. Please send them our way! We will share your photos on Facebook and let everyone know you support NJCTS. Send photos to email@example.com.
NJ Walks for TS promotes awareness, acceptance, action, and advocacy of Tourette Syndrome and its associated disorders. This event is about fun and empowerment for kids, by kids, and about kids. It is a chance to stand up and step out to break the stigma attached to TS and show your support for our friends, family, and neighbors living with this misunderstood neurological disorder. All funds raised support our various programs including education outreach, scholarships and awareness.


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Set in the heart of Loughton, being only a short walk of the Central Line station and High Road shops, is this three bedroom detached family home, located within the Staples Road primary school catchment. There is a 60ft west facing rear garden; two reception rooms; and a 12ft kitchen with an additional 12ft utility area. The property has potential to both extend and offer off street parking, subject to all the relevant planning consents being obtained.
10' 3" x 5' 6" (3.12m x 1.68m) Fitted carpet. Radiator. Ceiling light point. Understairs storage cupboard.
12' 9" x 12' (3.89m x 3.66m) Double glazed bay window to front aspect. Fireplace. Fitted carpet. Radiator. Ceiling light point. Coving.
12' x 11' 5" (3.66m x 3.48m) Double glazed window to rear aspect. Fitted carpet. Radiator. Ceiling light point. Coving.
11' 10" x 6' 10" (3.61m x 2.08m) Fitted with wall and base units. Sink with mixer tap. Integral oven, gas hob and chimney hood. Space for dishwasher. Worktops with tiled splashbacks. Double glazed window to rear aspect. Tiled flooring. Breakfast bar. Ceiling light point.
12' 4" x 5' 5" (3.76m x 1.65m) Space for appliances. Double glazed windows to front, side, and rear aspects. Tiled flooring. Double glazed door to rear garden.
Double glazed window to side aspect. Loft hatch. Fitted carpet. Storage cupboard. Ceiling light point.
12' 9" x 11' 11" (3.89m x 3.63m) Double glazed window to front aspect. Fitted wardrobes. Fitted carpet. Radiator. Spotlights.
11' 5" x 8' 10" (3.48m x 2.69m) Double glazed window to rear aspect. Radiator. Ceiling light point.
11' 11" x 6' 11" (3.63m x 2.11m) Double glazed window to rear aspect. Radiator. Spotlights.
5' 6" x 5' 4" (1.68m x 1.63m) Suite comprises of bath with wall mounted shower; pedestal wash basin; and close coupled WC. Tiled walls. Double glazed obscure window to front aspect. Radiator. Spotlights.
Steps up to front door with mature shrubs. Potential for off street parking subject to the usual consents being obtained. Gated side access to rear garden.
60' x 28' (18.29m x 8.53m) Commences with patio and steps leading to lawn with mature shrubs and trees.


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Today's HQ Trivia Questions & Answers : Previous Quizzes for Practice
HQ Trivia Practice Questions & Answers (15 Dec 2017)
Participants : 355k
Prize : $1500
Winners : 37 (Each one got $40.54)
- From which service did people in the 90's hear "you've got mail!"? AOL, Netflix, Kiki's Delivery service
- Veronica, Betty & Jughead are characters from which comic book franchise? East of west, Watchmen, Archie
- Crystals growing inside a hollow rock are known as? Geode, Moraine, Fizzgig
- "The natural" and "moneyball" are movies about which sport? Football, Basketball, Baseball
- Which sauce has pine nuts as an ingredient? Ponzu, Pesto, Tahini


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27 Mar Irrational thoughts vs Rational thoughts?
Rational Emotive Behavior Therapy
Rational Emotive Behavior Therapy (REBT) is a short-term form of therapy that helps you identify self-defeating thoughts and feelings, challenge the rationality of those feelings, and replace them with healthier, more productive beliefs. REBT focuses mostly on the present time to help you understand how unhealthy thoughts and beliefs create emotional distress which, in turn, leads to unhealthy actions and behaviors that interfere with your current life goals. Once identified and understood, negative thoughts and actions can be changed and replaced with more positive and productive behavior, allowing you to develop more successful personal and professional relationships.
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What color Bill? And who will place the sponsor decals?
Some type of Blue, with silver or white. I real like Sal's blue, but I don't want to use the same exact color. I'm sure stickers will go on at the track again. I have better luck finding someone that can do it there. Scott Horner came accross the country just to put stickers on for me Man I suck at putting them on Bill


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JCN Online Journals
Welcome the the Journal section of JCN Online. Here you can view every article from the current Journal of Community Nursing.
There are also extensive back issues available.
Please sign in or register to view the articles.
Journal details
Virtual reality
How exactly do you convey to students the difficulty of dealing with patients in their own homes? Here, Cathy Greaves, district and Queens Nurse, explains how simulated learning helped her students...


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Why a Label With Roots in London Is Betting on 3 Dallas Musicians
A burgeoning group of North Texas talent is taking advantage of a modern landscape where traditional boundaries are disappearing.
Cliff Simms is quietly sipping a pint at City Tavern in downtown Dallas, the very model of a British expat. He has lived in Dallas since 2009, after first visiting the year before on a business trip. Simms may be sitting across from me on this October night, but I know he isn't here right now. At least his mind isn't. It's in Manhattan, where singer-rapper Sam Lao is performing at the legendary SoHo club SOB's, as part of the Who's Next? Live showcase put on by taste-making hip-hop radio station HOT 97. He keeps tabs on Lao's performance from afar, gleaning details from texts and tweets.
Lao is in New York to promote herself, of course, and to a lesser extent the long-slept-on Dallas hip-hop scene; she's the first local rapper to be invited to the HOT 97 event. But Simms is so invested in what's happening at SOB's because she is also an ambassador of DEFDISCO, the Sheffield- and Dallas-based label he co-founded in 2014. While Lao is the most recent DEFDISCO signing, joining a burgeoning group of North Texas talent, if Simms had his way, she would have been the first. For whatever reason, the time wasn't right then. It is now—for signing with Simms' label, for breaking out of Dallas, for stardom, maybe.
"I think anybody who has worked in the music industry—and I don't mean this condescendingly, but at a level like London, New York, and LA, a major musical hub, and has been around well-established artists and international-selling acts—anyone who has had that kind of exposure and sees Sam Lao, and doesn't make those parallels, is either not very good at their job or an idiot," Simms says.
But Lao is just part of Simms and DEFDISCO's Dallas-centric strategy. She joins a stable that already included Kaela Sinclair, a singer-songwriter and touring member of French dream-pop act M83; soul singer Kirk Thurmond, who performs solo as well as fronting his band The Millennials; and Fort Worth rapper 88 Killa, who was a member of the popular local hip-hop collective Brain Gang, along with Lao, Blue, The Misfit, and Dr. Dre protégé Justin "Justus" Mohrle. Along with Lao, DEFDISCO also recently signed R&B vocalist Larry g(EE) and electro-pop singer LEV. And, in addition to a roster full of Dallas musicians possessing both marketability and stone-cold talent, the label is now armed with a worldwide distribution and marketing deal with ADA, the powerful, well-connected, independent-focused branch of the Warner Music Group.
At the heart of all of this is the savvy hand of the UK-born Simms. He worked with Sony and EMI, and overseas in Dubai, before coming back to the States to help the widow of Buddy Holly sell the catalogue of his music. While in Dallas, Simms began to freelance, assisting contacts from London with licensing and clearance contracts. Through his freelance gigs, he connected with future partner Barry Gilbey, founder of the influential house music label Choo Choo Records. Simms' background in publishing made him a valuable asset when they started DEFDISCO in 2014, with other partners Justin Williams and Paul Herron.
"I don't even look at it as a record company," Simms says, over his pint. "We are an artist agency. Instead of the mechanism on the back end being how many albums can we sell, it's who can we partner an artist with using this model? If you get the brand right, the record takes care of itself. We took a record label and an agency and welded it together. The face of it is a record label, but the engine is an ad agency."
It makes sense in a modern landscape where traditional boundaries are disappearing, and everyone is vying for the same consumer dollar. Lao, LEV, Larry g(EE), and the rest of Simms' Dallas signings need the massive awareness major brands bring, and brands need the immediacy that a song like Lao's feminist anthem "Pineapple" provides.
"There's no other medium like music that gets straight through that white noise and directly engages," Simms says. "Without getting corny, music is the universal language."
Sam Lao
For a few years now, after storming on the scene with her West Pantego EP, there has been no artist more destined to break out of Dallas' area code than Sam Lao. Her newest album, SPCTRM, will be officially released through DEFDISCO and distributed by ADA/Warner, with marketing support from Warner Music and Black Box LA (who have worked with Andra Day and Gary Clark Jr.). With a visual arts background, Lao understands the intersectionality of aesthetics and message, art and commerce, creating a polished sound that transcends the hip-hop framework of her music. Songs like the anti-catcalling anthem "Pineapple," "Fool's Gold," and "Kaleidoscope" (which features a hype, descriptive verse from 88 Killa, who encouraged her early musical pursuits) all shimmy with '90s summertime leisure while never losing Lao's innate authenticity. But it's the expansive track "Higher," which allows Lao the room to explore the full breadth of her vocals and emotive content, that showcases the real potential of what she can be—an artist capable of limitless appeal.
Holly Peyton, who performs under the name LEV, caught Simms' attention when she released her debut EP, Fear No Evil, in 2015. Blending influences from '80s art-pop stars such as Gary Numan, Human League, and Depeche Mode, Fear No Evil was confident and strange, a tapestry of dark, literary, synth-heavy pop songs. Maybe more than anyone, LEV represents the archetype DEFDISCO is interested in cultivating and growing, an artist with defined character, talent, and layered appeal. In fact, her sound is so polished and crisp, it's kind of hard to believe she hasn't already caught on in a big way. Peyton will release her first full-length, Glass Ceiling, midyear with marketing support from Warner Music and The Syndicate, who have worked with CHVRCHES and Spoon.
Larry g(EE)
When Larry g(ee) released his recent single "Don't Talk" (featuring Sam Lao and produced by DJ A1, who works with Erykah Badu), there was a noticeable shift from the classic sound g(EE) was known for, an amalgam of '60s soul and big band standards. The darker, moodier sound of "Don't Talk" was a departure, more resembling The Weeknd and FKA Twigs, showing a new inspiration from alternative soul and pop. But one thing that hasn't changed is his stage presence. g(EE)—born Larry Gayao—has always been known for his live performances, catching so much notice from his shows in Dallas that he appeared as the featured musical act on Jimmy Kimmel Live in 2012. Since then, he has been working on finding the right label deal that works for his evolving sound. Now that he's found that with DEFDISCO (he'll release something later this year), g(EE) provides a valuable veteran presence on the roster.


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JUST IN: THIS INTERESTING COMEDY FILM TO RELEASE FOR DIWALI ALONG WITH MERSALHome > Tamil Movies > Tamil Cinema News
By Arvind Sundaram | Oct 12, 2017
All the while, it was expected that Thalapathy Vijay's Mersal would be a solo release for the Diwali festival, but here comes one interesting new addition to the Diwali race.
Karthik Subbaraj's debut production film, Meyaadha Maan, starring Vaibhav, Priya Bhavani Shankar, under Rathna Kumar's direction is all set to hit the screens for Diwali on the 18th of October. The film was earlier scheduled to release on November 17th, but now the team have advanced the release date, by a month.
The official new poster of Meyaadha Maan, which indicates the Diwali release says, "Mersal aana kaalai varudhunga, kudave, thulli maanum varudhunga!"
There is also a speculation about Sasikumar's Kodi Veeran releasing for Diwali, but let us have to wait for an official confirmation. For now, there will be 2 Diwali releases.
RELEASE DATE OF MEYAADHA MAAN IS HEREHome > Tamil Movies > Tamil Cinema News
By Avinash Pandian | Oct 10, 2017
Here are a few notable quickies that might have missed your attention
1. GV Prakash's 100% Kadhal shooting to kickstart from tomorrow, 11th of October. The film is a remake of Telugu film 100% Love. Arjun Reddy fame Shalini Pandey plays the female lead in this remake.
2. Meyaadha Maan funded by Karthick Subbraj's Stone Bench Production gets an official release. The movie is slated to release on the 17th of November.
3. Shraddha Kapoor completes Prabhas' trilingual film Saaho's 1st Schedule. The highly rated Bollywood actress confirmed the same via Twitter, "1st schedule wrap on #SAAHO. Bittersweet feelings.Have had an amazing time shooting with a fabulous team. Felt so at home in #Hyderabad ❤"
*Tweet is not spell-checked
- Meyaadha Maan To Release On The 17th Of November
- Meyaadha Maan Starring Vaibhav And Priya Bhavani Shankar To Release On November 17, 2017.
- Karthik Subbaraj, Vaibhav And Priya Bhavani Shankar's Meyaadha Maan Audio To Release On September 27, 2017.
- Karthik Subbaraj's Debut Production Film Meyaadha Maan Planning To Release On 3rd November
- Karthik Subbaraj, Vaibhav And Priya Bhavani Shankar's Enna Naan Seiven Single From Meyaadha Maan To Release On August 25
- Meyaadha Maan Director Rathna Kumar Talks About His Experience With T. Rajendar At His Residence
- Meyaadha Maan Single Thangachi Song To Release On 7th August
- Karthik Subbaraj And Vaibhav's Meyaadha Maan Teaser Review
- ATMUS To Release Mersal In The USA. Full Theatre List Details Here
- Mersal Match Meter Game Reveals Details About Vijay's Characters In The Film
- AP International Acquires The Overseas Theatrical Rights Of Vijay's Mersal
- Actor Vijay Said To Have Performed The Mersal Pigeon Magic Trick By Himself.
- MERSAL Tribute To Thalapathy Fans! "Rasigane Thalaivan" | Lyricist Vivek
- 3500 Screens Worldwide? Will Vijay's MERSAL Set A New Record? | Atlee | TK 493
- MASS: What Is 80's Vijay's Name In Mersal? | Vijay | Atlee | TK 492
- "I Sweat It Out Only For Their Happiness" - Vijay Says
- Mersal - Maayon Bonus Track Full Lyrics | Vijay | Atlee | AR Rahman
- Disappointment For Thalapathy Vijay Fans | Mersal Trailer?
- Mersal First Show Chennai | Vijay | Samantha | Atlee
- "DO NOT Predict MERSAL Story!"- Art Director Muthuraj | Mersal Making | Vijay | RR09
- Mersal Teaser Hidden Details - Did You Witness Childhood Vadivelu? | TK 485
- Mersal FDFS - Double Treat For Alaporaan Thamzihan & Mersal Arasan | Vijay
- Mersal Second Promo - Videos
- MASSIVE RECORD: Mersal Teaser Hits 25 Million Views! | Vijay | Atlee


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Code 91890
Coral Carnitine supplement is recommended to boost one's energy and to improve immunity. The product enhances mental and physical performance and has fat burning effect.
Revitalisation Program, Weight Management Program
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Take one capsule twice a day with a meal.
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Form release: 180 Capsules


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ICON Sleeve With TENSAERLITE for MacBook Pro 15"- Thunderbolt (USB-C)
Securely Use:
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The shock-absorbing Tensaerlite bumper is molded and sewn to each side and provides maximum impact protection with minimal weight. Upper and lower compression panels are form-fitted to firmly secure your 15-inch MacBook Pro and faux-fur lined to keep your device looking new longer. The secure magnetic closure prevents unwanted slips and keeps out dust and debris. With easy access to all ports, you can charge your device while you protect it.
Complete surface and side protection for your 15-inch MacBook Pro.
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90% Polyester / 10% EVA