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Colors TV is launching a number of new television shows for its evening slot in 2017 and one of the most anticipated ones amongst them is Savitri Devi College and Hospital. The show is being produced by Rashmi Sinha.
The show is a medical drama and unlike other medical dramas which have aired previously on Indian television, this show's primary intent is to highlight the medical malpractices and health related issues cornering around a hospital. Moving on, lets find out more details about the show, its cast, story and timings.
- Swarda Thigale as Sanchi Mishra (Female Lead)–Known for her role in Majhe Mann Tujhe Jhale.
- Varun Kapoor as Dr. Veer Malhotra (Male Lead)– Known for his role in Sumit Sambhal Lega.
- Mohan Kapoor as Anand
- Shilpa Shirodkar as Jaya Mishra (Mother of Sanchi Mishra)– Known for her role in Silsila Pyaar ka.
- Kishwer Marhantt as Aparajita– Known for her role in Brahmarakhas.
- Neha Bagga–Known for her role in Ishq Da Kalma Baani.
- Sharan Kaur
- Vikram Sakhalar
Savitri Devi College and Hospital is a Delhi based hospital. The show intends to highlight the ill practices adopted by today's hospitals and the medical issues which is faced by common man and how they cope with it.
The narrative will be essayed through a lead female protagonist Sanchi Mishra, who will become an intern at the titular hospital. Later, how she fulfills her mother's dream and goes on to pinpoint the pitfalls of our medical system. On the another hand there is a medical genius, who is a hater of mankind. The serial will illustrate how his medical team handles the most complicated cases being treated by the doctor's differentiated diagnosis methodologies.
Also Check: Top 10 Highly Educated TV Stars in India
Multiple trailers of the show has been released by Colors Television and on the forefront the drama looks compelling.
Launch Date : May 15th, 2017
Days: Monday – Friday
Airtime: 06:00 PM
(Also, it is expected that the show's run time will be of 1 hour.)
Repeat Timings: Will be updated shortly.
For more details on this upcoming show and other Indian shows, stay tuned with us.


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2 Person Patio Glider Porch Swing Outdoor Garden Bench Rocking Chair Furniture. Unique blend of ventilating mesh fabric on an aluminum body for comfort and durability - Sturdy, gliding, supporting frame supports up to 350lbs for long-lasting use - Ideal for outdoor use with its weather-resistant material and design - Lightweight construction makes it particularly well-suited for the backyard or patio - Assembly required. You can't bring the beach home with you, but with this Outsunny mesh fabric patio glider chair, you can have that same contended feeling in your own backyard. Ideal for use pool side to get the perfect tan you've always wanted or in a shaded area for reading a book or sleeping.
The durable, quick-drying mesh fabric is woven for durability and the sturdy aluminum frame is sure to make you feel comfortable and stable while you lay quietly in the sun. The lightweight construction makes it especially easy to move about from place to place.
With its sturdy gliding frame, this chair invites comforting relaxation every time you use it. Color: Brown - Cushion Color: Gray - Overall Dimensions: 44"W x 19"D x 42"H - Seat To Floor: 17.5" - Seat Width: 40" - Seat Depth: 19" - Backrest Width: 40.2" - Backrest Height: 30" - Net Weight: 25lbs - Weight Capacity: 700lbs - Material: Sling fabric. We are committed to offering our customers value-oriented items in Outdoor & Patio, Home Goods, Health & Beauty, Sporting Goods, Pet Supplies, Toys & Hobbies.
We are always looking tobring new innovative items to our catalog so contact our Customer Service department with any suggestions you may have! Occasionally a warehouse is out of the product and the warehouse further from you will have to fulfill the order which could make the ETA up to 6 days.T processed the order yet, we can make the change. And you will be able to redirect your package(s).
For items that are defective or damaged, Aosom may require pictures to help us determine the appropriate next step, which may be replacement parts, or a replacement unit. In this day and age, we understand people?S time is valuable, so supplying pictures when you message us will help us assist you faster. The item "2 Person Patio Glider Porch Swing Outdoor Garden Bench Rocking Chair Furniture" is in sale since Thursday, March 17, 2016. This item is in the category "Home & Garden\Yard, Garden & Outdoor Living\Patio & Garden Furniture\Patio Chairs, Swings & Benches". The seller is "mhcorp" and is located in Wilsonville, Oregon. This item can be shipped to United States.

subset: commoncrawl


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I was wondering if there was a way to add a client ip to an address list once their bytes passed had exceeded a set limit.
The nearest I could see in MT is the dst-limit feature which uses packets passed - sadly I cannot see this being a very accurate method.
I notice that IPtables has a quota module available these days - maybe Mikrotik would consider adding this feature in?? I think it would be useful to a lot of us.


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Apply now to high school, elementary school, and 3 magnet preschools via www.go.cps.edu now through Dec. 13, 2019. https://go.cps.edu/about-gocps
Tag: SEES
Non-CPS Students: Register for 5-9th Grade CPS NWEA Testing
Non-CPS students who are interested in applying for 5th-9th grade CPS programs for Fall 2020 should be sure to take the CPS administered NWEA Map tests. CPS requires this qualifying test of reading and math to be taken by all non-CPS students applying to selective programs in those upper grades. Students can register by August 30 to take the tests on Sept. 21, 22, 28, 29 or Oct. 5 or 6. All students who test by those dates will be able to use the online GoCPS application system. All current CPS students will have their spring NWEA Map scores used for qualifications and rubric calculations. GoCPS applications are open 9/30/19-12/6/19.
Spring 2019- CPS Elementary Notifications & Links
First round CPS elementary school notifications were released at 5pm on Monday, April 22.
Academic Centers (accelerated 7th/8th grade programs in high schools) have cutoff scores published here: GoCPS AC Cutoff Scores 2019-2020
All other elementary Gifted or Classical School programs do not publish cutoff scores. These offers are "single choice" and you can only receive one offer of SEES (selective enrollment elementary schools).
Magnet, magnet cluster & open enrollment schools are strictly lottery based and will have waitlist numbers published. These are multiple offer programs so you can be offered multiple schools and accept one yet be offered another option later and can change your acceptance then.
CPS Elem from Fall 2017: Continuing the Discussion from CPSO
For those looking to discuss the 2018-2019 application cycle for CPS Elementary school options, feel free to check out the threads at CPSObsessed- Fall 2017: Applying to Kindergarten/Elementary School and continue your thoughts either in the comments below or on the School Forum pages.


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Playlist- 9/12/11
1. Architecture in Helsinki- Contact High
2. Phoenix- Lisztomania
3. The Arctic Monkeys- The Hellcat Spangled Shalalala
4. Silverchair- The Greatest View
5. The Wombats- Shock Goodbyes and P45's
6. Seth Sentry- The Waitress Song
7. 28 Days- What's The Deal?
8. Kasabian- Fire
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- Fri Jan 15, 2016 6:07 pm
- Forum: Relax'n discuss-stuff
- Topic: LED screen burn in.....
- Replies: 1
- Views: 3683
RE: LED screen burn in.....
Hello Andrew, I use LED monitors as well. But, this is looking strange for me. Did you ask your service center for the issue? Do you have any warranty or guarantee for the monitors? If any, please replace it. I have been using it about 6 months and keeping the power off when done. Can you describe t...
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text: Formal Graham Crackers $13.99 Description Graham Crackers dipped in white chocolate and dark chocolate to create tuxedos and formal gowns. Perfect for weddings, rehearsal dinners and formal occasions of any kind. Price is for a box of 12. Quantity Add to cart Category: Weddings Related products Formal Berries $19.99 Add to cart Formal Apples $5.00 View products Formal Oreos $13.99 Add to cart Berrylicious Gown Apple $5.00 Add to cart


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It may not be immediately apparent but one way of developing applications to run in Sakai2 is to use a Google Gadgets based approach. Sakai 2 uses iframes as its mechanism for placing tools, and this is the same mechanism as used by Google Gadgets. With some care in the way the Gadget is coded, the CSS used and the JavaScript its possible to create a Gadget for Sakai 2 that exposes functionality hosted from a departmental server. This page explains how to create a Google Gadget for Sakai 2.
If you are thinking of Sakai 3, then this supports Google Gadgets and Sakai Widgets, so work using this approach will be appropriate inside Sakai 3. If you are really thinking about functionality in Sakai 3, then the Sakai Widget approach will give you a much better user experience since it does not use any iframes.
When you develop a widget that you want to use inside Sakai, there are some general best practices on how to write it.
<link type="text/css" href="/devwidgets/filemanager/css/filemanager.css" />
<script type="text/javascript" language="JavaScript" src="/devwidgets/filemanager/lib/jquery.accordion.js" ></script>
<input type="checkbox" name="filemanager_option_public" id="filemanager_option_public" /> <label for="filemanager_option_public">Public</label>
When you have created a new site, you are able to add an external widget by following these steps:
In camtools 2008 the way you add gadgets is slightly different.
To add a gadget, you'll need to modify the widgets.js file (which is located under /tomcat/webapps/ROOT). In that file you can find a list of all the widgets/gadgets that are currently used by Camtools. Some of them are locally installed and others are remote.
Your own entry could look like this:
library : { description:"Cambridge Libraries Widget\r\n", iframe:1, url:"http://www.lib.cam.ac.uk/widget.cgi?origin=https://camtools.cam.ac.uk", name:"Cambridge Libraries", id:"library", height:350, portal:1, scroll: true }
description: It's a common best practice to always add a good description. This description will show up if you'll add the file
iframe: 1 or 0: if this is 1, an iframe will be used to insert the gadget. If you have an external widget this should be 1.
url: The url of the widget
id: The id of the gadget should be the same as the parent object name and should be unique.
height: Height of the iframe gadget (you don't need this property with a embedded widget.
portal: 1 or 0: Should be 1 if you want it to appear on the portal page.
scroll: true/false: If this is true, the iframe will have scrollbars when the content inside is bigger than the height property you've set.
To add the gadget to your own portal, you click on "customize" next to the "My Startpage".
There you should see your entry from the widget.js file and check the box next to it.
Just hit "update" and see if it correctly shows up on your start page.


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The hemagglutinin (HA) of fowl plague virus A/FPV/Rostock/34 (H7N1) carries two N-linked oligosaccharides attached to Asn123 and Asn149 in close vicinity to the receptor-binding pocket. In previous studies in which HA mutants lacking either one (mutants G1 and G2) or both (mutant G1,2) glycosylation sites had been expressed from a simian virus 40 vector, we showed that these glycans regulate receptor binding affinity (M. Ohuchi, R. Ohuchi, A. Feldmann, and H. D. Klenk, J. Virol. 71:8377–8384, 1997). We have now investigated the effect of these mutations on virus growth using recombinant viruses generated by an RNA polymerase I-based reverse genetics system. Two reassortants of influenza virus strain A/WSN/33 were used as helper viruses to obtain two series of HA mutant viruses differing only in the neuraminidase (NA). Studies using N1 NA viruses revealed that loss of the oligosaccharide from Asn149 (mutant G2) or loss of both oligosaccharides (mutant G1,2) has a pronounced effect on virus growth in MDCK cells. Growth of virus lacking both oligosaccharides from infected cells was retarded, and virus yields in the medium were decreased about 20-fold. Likewise, there was a reduction in plaque size that was distinct with G1,2 and less pronounced with G2. These effects could be attributed to a highly impaired release of mutant progeny viruses from host cells. In contrast, with recombinant viruses containing N2 NA, these restrictions were much less apparent. N1 recombinants showed lower neuraminidase activity than N2 recombinants, indicating that N2 NA is able to partly overrule the high-affinity binding of mutant HA to the receptor. These results demonstrate that N-glycans flanking the receptor-binding site of the HA molecule are potent regulators of influenza virus growth, with the glycan at Asn149 being dominant and that at Asn123 being less effective. In addition, we show here that HA and NA activities need to be highly balanced in order to allow productive influenza virus infection.
Influenza A and B viruses contain two spike glycoproteins, the hemagglutinin (HA) and the neuraminidase (NA). During the viral life cycle, both of these glycoproteins fulfill distinct functions in receptor binding and virus release which are crucial for establishing a productive infection (for a review, see reference 19). HA is the prototype of a class I transmembrane glycoprotein and is embedded in the viral membrane as a homotrimer (38). The HA monomer consists of a globular head region connected to a fibrous stalk domain (39). Both regions carry N-linked oligosaccharide side chains, with those attached to the stalk region being highly conserved and those at the tip of the molecule showing considerable variation in structure and number among different influenza A viruses. The tip of the globular region harbors the receptor-binding pocket, which mediates virus binding to sialic acid-containing receptors on the surface of host cells. N-glycans attached to the HA head domain in close proximity to this receptor-binding site have been suggested to modulate the receptor affinity (6, 10, 23). The HA of fowl plague virus (FPV; A/FPV/Rostock/34 [H7N1]) has two N-glycans flanking the receptor-binding pocket (17). They have been shown to significantly decrease the receptor-binding activity of transiently expressed FPV HA. HA mutants lacking either one or both of these glycans have an enhanced hemabsorbing activity as evidenced by an almost irreversible tight binding to erythrocytes (26).
NA is anchored in the viral envelope as a mushroom-shaped homotetramer with type II membrane topology (3). NA acts as a receptor-destroying enzyme by catalyzing the removal of sialic acids from viral and cellular components. NA activity has therefore been shown to promote the release of progeny viruses from host cells and to prevent virion aggregation (4, 11, 20, 28).
When different natural and laboratory-derived influenza viruses were analyzed for their HA and NA composition, it was striking to see that some combinations of antigenic subtypes occurred quite frequently while others were never detected (18, 32). In the latter case, the failure to produce stable high-yield reassortant strains with some HA and NA combinations has been attributed to an incompatibility between the opposing activities of these two glycoproteins leading to viral aggregation. The importance of a functional HA and NA match for productive infection was also suggested in a very recent study indicating that changes in HA receptor-binding activity occurring during adaptation to a new host are accompanied by concomitant changes in the NA sequence (22).
Since this concept of matching HA and NA combinations had so far only been deduced from analyses of naturally occurring viruses, our aim was to characterize the requirements for an effective interaction of HA and NA in more detail on the molecular level. To this end, we generated recombinant influenza viruses in which HA mutants lacking either one or both tip glycans (26) were combined with different NA subtypes and the growth properties of the resulting viruses were assayed in cell culture. For the production of these recombinant viruses, we used a recently described RNA polymerase (PolI)-based system (30, 40). We show that, as a consequence of the enhanced receptor affinity of the mutated HA, progeny virus release from host cells was significantly restricted, resulting in limited cell-to-cell spread. Therefore, the N-glycans at the tip of HA appear to be potent regulators of virus growth in cell culture. Interestingly, this effect was dependent on the nature of the accompanying viral NA. The high-activity N2-subtype NA was able to partly overcome increased binding of carbohydrate-deficient HA, while the low-activity N1-subtype NA was not. By employing specifically designed recombinant viruses, we provide direct experimental evidence that a functional balance of HA and NA is an important determinant of productive influenza virus infection.
Cells and viruses.Kidney cells from African green monkeys (CV1) were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal calf serum (FCS) (Life Technologies GmbH, Karlsruhe, Germany), Madin-Darby bovine kidney (MDBK) cells were kept in DMEM with 4.5 g of glucose per liter supplemented with 10% FCS, and Madin Darby canine kidney cells (MDCK) were grown in minimal essential medium (MEM) containing 10% FCS. All cells were maintained at 37°C and 5% CO2.
Two reassortants of influenza viruses, A/WSN/33 (H1N1) and A/Hong Kong/8/68 (H3N2), were used. The reassortant WSN-HK (33) contains the N2-subtype NA gene of the Hong Kong virus and the residual genes of the WSN virus, and the reassortant HK-WSN (9) contains the H3-subtype HA gene of the Hong Kong virus and residual genes of the WSN virus. Both reassortants were amplified in 11-day-old embryonated chicken eggs.
Construction of plasmids.The hemagglutinin gene of FPV (A/FPV/Rostock/34) (H7N1) was amplified from the pA11SVL3 vector (26) by PCR using the oligonucleotide GGCCGCTCTTCTATTAGTAGAAACAAGGGTG as a forward primer and the oligonucleotide GGCCGCTCTTCGGCCAGCAAAAGCAGGGGATACAAAATGAACACTCAAATCC as a reverse primer. Both oligonucleotides contained SapI restriction endonuclease recognition sites. PCR products were digested with SapI and ligated in the viral genome-sense orientation into the SapI sites of the PolI-SapI vector, resulting in the construct PolI-SapI/HA. The PolI-SapI vector (kindly provided by Adolfo Garcia-Sastre, New York, N.Y.) is a derivative of the pPolI-RT plasmid described earlier (30) and contains a truncated version of the human PolI promoter and a hepatitis virus delta ribozyme separated by SapI sites. Expression plasmids pHMG-PB1, pHMG-PB2, pHMG-PA, and pHMG-NP, encoding the proteins of the influenza virus polymerase complex under the control of a hydroxymethylglutaryl-coenzyme A reductase promoter were kindly provided by J. Pavlovic (University of Zürich, Zurich, Switzerland). The N-glycosylation sites in FPV HA at positions 123 and 149 were inactivated using the Quickchange mutagenesis kit (Stratagene, Amsterdam, The Netherlands) according to the manufacturer's protocol. Threonine-125 was exchanged for alanine using the oligonucleotide GGAATAAGGACCAACGGCGCCACTAGTGCATGTAGAAGATCAGG as a forward primer and the oligonucleotide CCTGATCTTCTACATGCACTAGTGGCGCCGTTGGTCCTTATTCC as a reverse primer to obtain the G1 mutant of FPV HA (construct PolI-SapI/G1). Primers contained a NarI restriction site to confer a genetic tag to the mutated HA sequence. Similarly, serine-151 was exchanged for glycine using the oligonucleotide CCTGTCAAATACAGACAATGCCGGCTTCCCACAAATGACAAAATCATA as a forward and the oligonucleotide GTATGATTTTGTCATTTGTGGGAAGCC-GGCATTGTCTGTATTTGAC AGG as a reverse primer, resulting in the G2 mutant of FPV HA (construct PolI-SapI/G2). These primers contained an NaeI genetic tag site. For generation of the double mutant G1,2 HA vector (construct PolI-SapI/G1,2), Quickchange mutagenesis was performed on the PolI-SapI/G1 plasmid with the latter primer pair. Thus, the G1,2 mutant HA sequence was modified to include both the NarI andNaeI genetic tag sites. To distinguish between HA from authentic FPV and plasmid-based wild-type FPV HA, the latter sequence was modified by the introduction of a PvuII site at position 1149 using the forward primer GGAGAAGGAACTGCAGCTGACTACAAAAGCACCCAATCGG and the reverse primer CCGATTGGGTGCTTTTGTAGTCAGCTGCAGTTCCTTCTCC in the Quickchange mutagenesis procedure.
Rescue of recombinant viruses.CV1 cells were seeded in 6-cm dishes and grown to about 60 to 70% confluency. Cells were then transfected with plasmids pHMG-PB1 (1 μg), pHMG-PB2 (1 μg), pHMG-PA (1 μg), and pHMG-NP (2 μg) to express the influenza viral polymerase complex and with the PolI-SapI plasmid encoding the respective versions of the FPV HA sequence (5 μg). Transfection was performed using the Superfect transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions. At 36 h after transfection, the cells were infected with either the WSN-HK (H1N2) or the HK-WSN (H3N1) influenza virus reassortants at a multiplicity of infection (MOI) of 2. Progeny viruses were harvested at 18 h postinfection.
In infections with the HK-WSN helper virus, CV1 cells were treated with 10 mU of Vibrio cholerae neuraminidase (VCNA; Behring, Marburg, Germany) per ml of cell culture medium for 1 h at 37°C prior to virus harvest. Selection for recombinant viruses was then done using a specific neutralizing anti-H3-HA serum. To this end, progeny viruses from rescue experiments were adsorbed to MDBK cell monolayers for 1 h on ice, cells were washed two times with ice-cold PBS++ (135 mM NaCl, 2.5 mM KCl, 6.5 mM Na2HPO4, 1.5 mM KH2PO4, 1 mM CaCl2, 0.5 mM MgCl2 [pH 7.2]) and grown in FCS-free DMEM supplemented with 0.2% bovine serum albumin (BSA; ICN, Aurora, Ill.) containing 0.05% serum directed against the X31 strain of influenza virus (kindly provided by Peter Palese, New York, N.Y.). When the reassortant WSN-HK was used as a helper virus, selection was achieved by passaging rescue supernatants on MDBK cell monolayers in the absence of trypsin. Infected MDBK cell monolayers were then monitored for the appearance of liquid plaques for the next few days. Recombinant viruses were purified by three plaque passages on MDBK cells under selection conditions. For the production of virus stock solutions, recombinant viruses were amplified in MDBK cell monolayers seeded in 10-cm dishes.
Genotypic characterization of recombinant viruses.Plaque-purified recombinant viruses were used for the infection of MDCK cells seeded in 6-cm dishes. At 2 to 3 days postinfection, supernatants were collected and cleared of cellular debris by centrifugation at 2,000 × g. Viruses were subsequently pelleted from the supernatants by ultracentrifugation at 100,000 × g for 30 min. RNA was isolated from the virus pellet in a final volume of 50 μl of highly purified water by means of the High Pure RNA isolation kit (Roche Molecular Biochemicals, Mannheim, Germany) following the manufacturer's instructions. The isolated viral RNA (10 μl) was then subjected to reverse transcription (RT)-PCR employing the Titan One Tube RT-PCR system, supplied by Roche Molecular Biochemicals. The primers used in this procedure were GGCCAGTCCGGACGGATTGATTTTC (forward) and ATAGTGCACCGCATGTTTCCG (reverse) for wild-type (WT) plasmid-derived HA and GTATCAAATGGACCAAAGTAAAC (forward) and CGCAATTGGCATCAACCTGCACATCGC (reverse) for glycosylation-mutant forms of FPV HA. RT-PCR products were digested with PvuII (WT-HA), NarI (G1 mutant),NaeI (G2 mutant), or NarI and NaeI (G1,2 mutant), and cleavage products were examined by electrophoresis in a 1.4% agarose gel.
Phenotypic characterization of recombinant viruses.MDCK cells (2 × 106) were infected with recombinant viruses at an MOI of 2. At 8 h after infection, the cells were washed with phosphate-buffered saline (PBS), and 20 μCi of Redivue Pro-mix l 35S in vitro cell labeling mix (Amersham Pharmacia Biotech Europe GmbH, Freiburg, Germany) was added in 2 ml of MEM lacking methionine and cysteine. After 12 h, radioactively labeled viruses were pelleted from the supernatants. Viruses were lysed in 500 μl of radioimmunoprecipitation buffer (150 mM NaCl, 1% Triton X-100, 0.1% sodium dodecyl sulfate [SDS], 1% deoxycholate, 10 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 10 mM iodoacetamide, 5,000 U of aprotinin, 20 mM Tris-HCl [pH 8.8]). FPV HA was immunoprecipitated from the lysate by adding a monoclonal FPV HA-specific antibody (1:250) and 30 μg of protein A-Sepharose (Sigma, Deisenhofen, Germany). One half of the precipitated HA was digested for 6 h with 500 U of peptide:N-glycosidase (PNGase) F (New England Biolabs Inc., Schwalbach, Germany), while the other half remained untreated. Samples were run on SDS–10% polyacrylamide gel electrophoresis (PAGE), and HA bands were visualized by fluorography.
Analysis of virus growth.For growth curves, MDCK cell monolayers were infected with recombinant viruses at an MOI of 0.001 in PBS containing 0.2% BSA for 1 h. Unbound viruses were washed away with PBS–0.2% BSA, and serum-free MEM–0.2% BSA was added. Cells were incubated at 37°C under 5% CO2. From then on, HA titers in the supernatant were periodically monitored with chicken red blood cells (1% in saline).
For plaque assays, confluent MDCK cell monolayers in 6-cm dishes were infected with 10-fold dilutions of recombinant viruses in a total volume of 1 ml of PBS–0.2% BSA for 1 h. Cells were washed with PBS–0.2% BSA and covered with an overlay of MEM containing 0.5% purified agar (Oxoid Ltd, Basingstoke, Hampshire, England), 0.02% BSA, and 0.001% DEAE-dextran. Cells were incubated at 37°C under 5% CO2, and plaques were stained 3 days postinfection with 0.1% crystal violet in a 10% formaldehyde solution.
Determination of viral NA activity.The total protein content of viruses was determined using the BCA protein assay reagent (Pierce, Rockford, Ill.) following the supplier's instructions, with BSA serving as an internal standard. NA activity was measured with 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (MU-NANA) (Sigma) as a substrate (36). Defined amounts of viral proteins were diluted in 100 μl of 0.1 M sodium acetate buffer (pH 5.5). These mixtures were then incubated with 10 μl of 1 mM MU-NANA for 20 min at 37°C. The reactions were stopped by adding 1 ml of stop buffer (133 mM glycine, 60 mM NaCl, 40 mM Na2CO3 [pH 10.0]). The fluorescence of the released chromophore 4-methylumbelliferone was determined with a Perkin-Elmer luminescence spectrometer (λexc = 365 nm, λem = 450 nm) and was taken as a measure of the viral NA activity.
For the analysis of HA and NA incorporation, viruses were grown in MDCK cells in the presence of 50 μCi ofd-[6-3H]glucosamine (Amersham Pharmacia) for 20 h. Viruses were pelleted from the culture medium by centrifugation at 100,000 × g and applied to SDS-PAGE on a 10% gel. Protein bands were visualized by fluorography and excised from the gel. Radioactivity incorporated in HA and NA bands was measured by liquid scintillation counting.
Virus elution from chicken erythrocytes.Virus stocks were diluted serially in PBS, and 50-μl aliquots of these dilutions were incubated with 50 μl of chicken erythrocytes (1% in saline) on ice for 1 h in V-bottomed microtiter plates. Thereafter, the plates were transferred to 37°C, and the precipitation of agglutinated erythrocytes was monitored periodically for the next 24 h.
Release of progeny viruses from host cells.Confluent MDCK cell monolayers were infected with recombinant viruses at an MOI of 5. At 12 h postinfection, virus titers in the culture medium were examined by plaque assay on MDCK cells as described above. In parallel, MDCK cells equally infected for 12 h were treated with 25 mU of VCNA per ml of medium for 1 h at 37°C in order to release all budded viruses from the cell surface. Again, virus titers in the culture medium were determined in a plaque assay on MDCK cells. Infections for plaque tests were done on ice to inhibit VCNA activity. Virus titers obtained in the presence of VCNA were regarded as the maximum virus yield and were therefore set at 100%.
Generation of recombinant viruses differing in HA glycosylation and neuraminidase subtypes.To investigate the possible effects of the oligosaccharides at Asn123 and Asn149 of FPV HA on the growth of intact viruses, glycan attachment sites were abolished from the HA sequence either individually or simultaneously by site-directed mutagenesis (Fig. 1). The HA wild-type and mutated cDNAs were placed in the genomic (antisense) orientation under the transcriptional control of a truncated version of the human PolI promoter so as to ensure the generation of a precise 5′ end of the transcript (25, 40). The correct 3′ end was brought about by a hepatitis virus delta ribozyme sequence included in the PolI-HA plasmid. For analytical reasons (see below), endonuclease restriction motifs were introduced as tag sites in the HA cDNAs. A PvuII site was added at position 1150 to the WT-HA cDNA. HA mutants G1 and G2 were modified by the introduction of a novel NarI site at position 445 and a novel NaeI site at position 523, respectively. Correspondingly, HA mutant G1,2 bears both artificialNarI and NaeI sites (Fig.2A). In transfected CV1 cells, intranuclear PolI-based transcription produces a virus-like HA RNA gene that is encapsidated and amplified by the proteins of the influenza virus polymerase complex (PB1, PB2, PA, and NP) translated from expression plasmids which had been cotransfected along with the PolI-HA vector (30).
To obtain recombinant viruses, CV1 cells were then infected with helper viruses. Two different reassortants of strains A/WSN/33 (H1N1) (WSN) and A/Hong Kong/8/68 (H3N2) (HK) were chosen for this purpose. The HK-WSN (H3N1) reassortant has all WSN genes except for the HA gene of subtype H3, which stems from the Hong Kong strain. Recombinants created with this reassortant as helper virus therefore carried the FPV HA gene within the context of the residual WSN genes. Selection for recombinants was achieved by passaging supernatants from rescue experiments on MDBK cells in the presence of a neutralizing anti-H3-HA serum, which specifically blocked the growth of the HK-WSN helper while leaving the recombinant FPV HA viruses unaffected.
The second helper virus used, WSN-HK (H1N2), contains all the WSN virus genes with the exception of the N2-subtype NA gene, which is derived from the Hong Kong virus. Tissue culture supernatants obtained with this reassortant as a helper virus were passaged in MDBK cells in the absence of trypsin. Because FPV HA is cleaved by the cellular protease furin (34), only the recombinant virus propagated under these conditions, whereas helper virus growth was inhibited in the absence of trypsin. The procedure described represents a novel system for the efficient selection of recombinant influenza viruses, taking advantage of specific functional properties of the FPV HA.
Following this approach, we rescued an N1 series and an N2 series of recombinant viruses, both carrying the glycosylation mutants of FPV HA within the WSN background: FPV/N1 recombinant viruses were derived from the HK-WSN helper, while FPV/N2 recombinants were derived from the WSN-HK helper. Rescued viruses were purified by three plaque passages on MDBK cells.
Molecular characterization of recombinant viruses.The recombinant identity of the viruses obtained in rescue experiments was confirmed by RT-PCR analysis of viral RNA. To this end, isolated viral RNA was used as a template for RT-PCR with two sets of HA-specific primers encompassing the introduced genetic tag sites mentioned above (Fig. 2). RT-PCR products were subjected to restriction analysis with the respective endonucleases and analyzed by agarose gel electrophoresis. With RNA of the WT/N1 recombinant virus, RT-PCR yielded a product of about 780 nucleotides which was sensitive toPvuII treatment, whereas no cleavage occurred when FPV RNA was applied. The presence of the genetic tag sites was also confirmed in the recombinants containing HA mutants. RT-PCR products obtained from the G1/N1 virus were cleaved by NarI, those from the G2/N1 virus were cleaved by NaeI, and those from the G1,2/N1 mutant were sensitive to both NarI and NaeI. Again, no sensitivity to these enzymes was seen with RT-PCR products generated from FPV RNA. Identical results were obtained when viruses of the N2 series were assayed (data not shown). Thus, restriction analysis clearly revealed that the plasmid-based mutated FPV HA genes had been stably integrated into the rescued viruses.
Next, it was important to prove that N-glycans are in fact missing from the HA protein of the respective virus mutants. For this reason, HA was isolated by immunoprecipitation from radioactively labeled virus produced in MDCK cells. When examined by SDS-PAGE, the HA1 subunits from the G1 and G2 mutant viruses showed a reduced molecular mass compared to wild-type HA1. A larger reduction was observed with the G1,2 recombinant. PNGase F treatment confirmed that the differences in molecular weight resulted from loss of oligosaccharides (Fig.3; data are shown for N2 recombinants only but were identical for viruses of the N1 series).
Growth of recombinant viruses in cell culture.In view of the results obtained by solitary expression of HA glycosylation mutants in CV1 cells described above (26), it was of great interest to see if the lack of the tip glycans had an effect when the mutated HA constitutes an integral part of intact virions. MDCK cells were therefore inoculated at a low MOI with recombinant viruses, and the emergence of progeny viruses was monitored. Within the N2 series, wild-type as well as G1 and G2 viruses grew equally well on MDCK cells (Fig. 4). Only growth of the G1,2 mutant was moderately affected. However, with viruses of the N1 group, the loss of oligosaccharide side chains from the HA tip had striking effects on virus growth depending on the position and number of the deleted glycans. Titers obtained with the G2 mutant were reduced about fourfold compared to those reached by the WT/N1 and G1/N1 viruses. With a more than 20-fold reduction in virus titers and a delayed onset of virion release (see also Fig. 8), the G1,2/N1 virus exhibited a highly attenuated phenotype in MDCK cells.
The studies on the growth characteristics were extended by performing plaque assays on MDCK monolayers to track cell-to-cell spread of mutant viruses (Fig. 5). Results obtained by this approach closely corresponded to those described above for the growth curves. Within the N2 group, only the spread of the G1,2 mutant was inhibited. Yet again, there was a marked reduction in plaque size within the N1 group which was very distinct with the G1,2 mutant and less pronounced with the G2 mutant, demonstrating impaired cell-to-cell spread of these viruses.
These results indicate that N-glycans at the HA tip promote influenza virus replication in cell culture. Moreover, we could show that NA as well has a crucial impact on virus growth. This is evident from the finding that only the N2 subtype, but not N1 NA, was capable of abrogating the downregulating effects of missing N-glycans.
Release of recombinant viruses from receptors.The data described so far suggested that the N1 series of recombinants differs from the N2 series in the efficiency of release from receptors. We therefore first compared the neuraminidase activities of recombinants WT/N1 and WT/N2 with MU-NANA as the substrate. The results shown in Fig. 6 indicate that neuraminidase activity is about six times higher in the N2 recombinant than in the N1 recombinant. In parallel, the HA and NA content of recombinant viruses was assayed by applying radioactively labeled virions to SDS-PAGE (Fig.6). Liquid scintillation counting of excised protein bands revealed that the ratio of HA to NA in both virus types was about 6:1. The observed differences in NA activity are therefore not due to varying incorporation of NA molecules into virions but obviously reflect the weaker enzymatic activity of N1 NA.
We then analyzed elution of viruses from erythrocytes. Viruses were adsorbed to chicken erythrocytes at 4°C, and release at 37°C was subsequently monitored (Fig. 7). Wild-type and G1 viruses of the N1 series eluted quickly from erythrocytes, with elution being complete after 2 h. However, the G2/N1 virus eluted to only about 50% even after 6 h of incubation at 37°C, and elution of the G1,2/N1 virus was totally blocked. This clearly demonstrated that the NA activity of N1 viruses was too weak to overcome the high receptor affinity of the G2 and G1,2 mutant HA. By contrast, NA activity in N2 viruses was able to elute wild-type, G1, and G2 viruses quantitatively from the erythrocytes within about an hour and only failed to fully release G1,2 viruses.
Incomplete release of progeny viruses from infected cells might be overcome by VCNA treatment. Therefore, MDCK cells were infected with recombinant viruses at an MOI of 5. Before virus harvest, cells were treated with VCNA to quantitatively elute virus from the cell surface. Virus titers were determined by plaque assay on MDCK cells. Compared to virus release in the presence of VCNA, G2/N1 and G1,2/N1 were released in the absence of VCNA to only about 22 and 6%, respectively (Fig.8). Release of G1/N1 was only moderately affected. Within the N2 NA group, elution of G1,2 closely resembled that of G2/N1, while the other members of this group were not significantly restricted in their release from host cells. By adding VCNA to the overlay of plaque assays with G2/N1 and G1,2/N1 viruses, it was possible to overcome the size restrictions described above (data not shown). This clearly indicated that VCNA was able to promote the spread and multicycle growth of these viruses.
HA-mediated attachment of influenza viruses to sialic acid-containing receptors on the host cell surface is the initial step in infection. Influenza virus HA contains at the tip a narrow crevice lined with highly conserved amino acids. By its ability to specifically bind sialic acids, this crevice has been identified as the receptor-binding site (7, 31, 37). The precise structure of this HA domain is known to be of crucial importance for the process of virus binding to its receptor. Accordingly, single-amino-acid substitutions in the binding pocket can result in altered receptor-binding specificity and altered host range of the viruses (1, 5, 35). Furthermore, in our previous study employing vector-expressed FPV HA, we could show that oligosaccharides flanking the binding site modulate receptor affinity (26). The aim of the present study was to evaluate the impact of each individual N-glycan at the FPV HA tip on the growth of intact viruses. To address this question, we generated recombinant influenza viruses containing the oligosaccharide-deleted HA mutants. Our studies demonstrate that the glycans flanking the receptor-binding pocket are potent regulators of virus growth in cell culture. The oligosaccharide attached to Asn149 (absent in mutant G2) plays a dominant role in controlling virus spread, while that attached to Asn123 (absent in mutant G1) is less effective. Growth of viruses lacking both N-glycans was found to be reduced in cell culture due to restricted release of progeny viruses from infected cells. These findings on the growth of recombinant viruses are an important extension of our previous work investigating the receptor interaction of transiently expressed HA. The results presented here provide experimental evidence for a distinct regulatory function of individual N-glycans located at the HA tip in the viral life cycle. By sequentially removing N-glycans from the vicinity of the HA receptor-binding site, we have delineated a novel approach to specifically generating influenza viruses with gradually greater degrees of attenuation in cell culture.
By removing terminal sialic acid residues from oligosaccharide side chains of glycoconjugates, the viral NA acts as a receptor-destroying enzyme in influenza viruses (3, 19). When NA activity was blocked by either antibodies (4), inhibitors (12, 27), or temperature-sensitive mutations (28), formation of large viral aggregates on the surface of infected cells was observed, as with virus lacking NA either partly (24) or completely (20). Accordingly, viral NA is regarded as an important factor for the release of progeny virus from host cells, promoting the efficient progression of an infection. In light of this, it was of special interest to examine how different NA subtypes affect the attenuated phenotype of recombinant viruses lacking N-glycans at the HA tip. Several N1 NAs have a deletion in the stalk region that is most extensive with FPV NA (14). NA enzymatic activity has been reported to vary according to the length of the stalk region of the molecule, with NA species containing a deletion in the stalk having lower activity (2, 8, 21, 22). By choosing appropriate helper viruses, we generated recombinants in which the HA mutants were combined with either the WSN virus NA (N1 subtype) containing a stalk deletion or the Hong Kong virus NA (N2 subtype) that has no deletion. When assayed for neuraminidase activity, recombinant viruses carrying N2 NA exceeded those with N1 NA at least sixfold. Thus, our set of recombinants was ideally suited to analyze in depth the impact of different NA activities on the growth of mutant influenza viruses specifically designed to show distinct receptor-binding activities. Using this system, we were able to demonstrate that the growth behavior of HA mutant viruses is governed by the nature of the accompanying viral NA.
Among the viruses with high-activity N2 NA, growth restriction was observed only when the G1,2 mutant was present, showing the highest receptor affinity, while recombinants containing G1 and G2 grew essentially like virus carrying wild-type HA. Yet the situation was different with viruses containing the low-activity N1 NA. Here, the growth of G1,2 mutant viruses was significantly impeded in cell culture due to restricted release from host cells. This effect was less pronounced with G2 mutant viruses but still evident. Obviously, unlike N2 NA, the lower-activity N1 NA is not able to overcome the high-affinity interaction of G1,2 and G2 HA with its receptor.
Hence, our data clearly point out that, for the establishment of productive infection, influenza viruses are strictly dependent on a highly balanced action of HA and NA. An increase in receptor-binding affinity apparently needs to be accompanied by a concomitant increase in the receptor-destroying activity of the viral NA. Otherwise, the enhanced receptor binding is a serious disadvantage in the late stage of infection because it prevents the release of progeny viruses from host cells. The need for such a match of HA and NA activities had so far only been deduced from studies analyzing natural virus isolates or laboratory-generated reassortants (15, 16, 22, 32). Taken together, our data represent the first concise study of the functional interrelationship of distinct HA and NA species and provide experimental evidence for the strict requirement of a fine tuning of HA receptor-binding and NA receptor-destroying activity in order to allow efficient influenza virus propagation (Fig.9).
There is evidence that the N-glycans flanking the receptor-binding site not only modulate receptor affinity but also control receptor specificity. Thus, subtype H1 influenza virus strains with an oligosaccharide in such a position have been shown to bind preferentially to α2,3-linked neuraminic acid, whereas mutants lacking this oligosaccharide had a preference for the α2,6 linkage (10, 13). Furthermore, it has been shown recently that glycans carrying neuraminic acid in α2,3 or α2,6 linkages gain access to the receptor-binding pocket from opposite sides (7). Steric hindrance by a glycan adjacent to the receptor-binding site may therefore be a determinant of receptor specificity. Finally, the number and structure of N-glycans neighboring the receptor-binding pocket have been suggested to determine the host range and pathogenicity of influenza viruses (6, 10, 29). In view of these findings, it will now be interesting to employ our panel of recombinant viruses to elucidate the contributions of individual HA tip glycans to tissue tropism and host range.
We are grateful to Peter Palese and Adolfo Garcia-Sastre for kindly providing the anti-H3-HA serum, the reassortant helper viruses, and the PolI-SapI vector.
This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 286) and from the Fonds der Chemischen Industrie. T.W. was a recipient of a fellowship of the Deutsches Krebsforschungszentrum (Infektionsforschung, AIDS-Stipendienprogramm).
- Received 15 December 1999.
- Accepted 25 April 2000.
- Copyright © 2000 American Society for Microbiology


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You have to remove the content in question, immediately, before even verifying the truth of the complaint SOPA is even more draconian. I know my books are "pirated" by illegal downloaders. This isn't going to stop pirates; they don't use or need domain names they'll use rotating IP, anyway, and go off shore.
i know there are a lot of people out there that are looking for the same thing but i really do have a lot to offer inbetween the sheets and hope that this actually works out for us.
But in my mind, a master/slave agreement or relationship is one where the slave has given up all control to the master.
What I would have answered a year ago, is not the same as what I say today nor it remain the same next year as I learn more and am exposed to more.
What was Palin's nickname on the high school basketball team?
In a few years, if you decide that you truly want to have (and not just because someone says you should), then consider adoption at that point.
Just have needs and wants that I can satisfy for you. Hot women seeking casual sex South Ayrshire)– profit.
MWM seeks older 35 y o plus MWF for ongoing discreet friendship Man wants married looking for sex and ankles attached to the bar. The acidity is the issue for me apparently, as the other things I gave up or reduced were also high acid foods. He couldn't wait, though, because he has the self control of a year old and wants instant gratification. I still have caffeine, I drink chai tea, but not daily. He thinks that sounds more adult, but his behavior indicates otherwise. He should have waited until your guest left to have it out with you. But it affects not only their sexual life, but their daily existence and perhaps even their more spiritual side. I say that I was out with a friend and did not good tea options, so I ordered a latte about 6 weeks after not having any. Now, mind you, this is still consensual and based on understanding of limits, etc.yes tipping over is a small concern but its not a fall nor a particularly painful one if you don't fall on a table corner or something.


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WordPress Site Editor Plugin Path Traversal Vulnerability [CVE-2018-7422]
CVE number – CVE-2018-7422
A vulnerability in the Site Editor plugin for WordPress could allow an unauthenticated, remote attacker to access sensitive information on a targeted system.
The vulnerability is due to insufficient sanitization of user-supplied input submitted to the ajax_path parameter that is processed by the :-
code of the affected software. An attacker could exploit this vulnerability by sending a request that submits malicious input to the targeted system. A successful exploit could allow the attacker to access arbitrary files on the system, which could be used to conduct further attacks.
Proof-of-concept (PoC) code that demonstrates an exploit of this vulnerability is publicly available.
WordPress.org has not confirmed the vulnerability and software updates are not available.
To exploit this vulnerability, an attacker must send a request that submits malicious input to the targeted system, making exploitation more difficult in environments that restrict network access from untrusted sources.
As of March 27, 2018, the Site Editor plugin for WordPress has been closed and is no longer available for download.
Administrators are advised to contact the vendor regarding future updates and releases.
Administrators are advised to allow only trusted users to have network access.
Administrators are advised to run both firewall and antivirus applications to minimize the potential of inbound and outbound threats.
Administrators may consider using IP-based access control lists (ACLs) to allow only trusted systems to access the affected systems.
Administrators can help protect affected systems from external attacks by using a solid firewall strategy.
Administrators can apply Snort SID 47424 to help prevent attacks that attempt to exploit this vulnerability.
Administrators are advised to monitor affected systems.
At the time this alert was first published, WordPress.org had not released a security advisory.
At the time this alert was first published, WordPress.org had not released software updates.


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Johnny Tremain, a young apprentice silversmith, is caught up in the danger and excitement of Boston in the 1770s, just before the Revolutionary War. Johnny can't help being swept along by the powerful currents that will lead to the Boston Tea Party and the Battle of Lexington. But even more gripping than living through the drama of Revolutionary Boston is the important discovery Johnny makes about his own life.
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- lifestyle -
Kanye West Is Working On A Song Titled "Piss On Your Grave"
Kill it with pee.
08 . 19 . 14
Ok, here's a bit of news, not surprising at all, to pep up your Tuesday. For better or worse I'm one of those people who eagerly awaits the latest official release from Kanye West. This idea of the rapper's possible collaboration with Paul McCartney is too enticing not to pay attention to. Today The Huffington Post did shed at least a little light on what fans can expect from Yeezus. After reaching out to West's reps for comment on the potential McCartney collab, the online publication confirmed that West is working away on new material, including a track entitled "Piss On Your Grave." Is anyone really shocked by the title? His team also declined to comment on whether or nit the artist was working alongside the iconic Beatle.
McCartney previously said in an interview that he thought about adding a rapper to his song "Appreciate." The only rap guest features he considered were Kanye West and Jay Z.
So there you go, a bit of not at all astounding news to share on your way out from the office.


text:
Previous Challenge Entry (Level 2 – Intermediate)
Topic: First (as in original) (01/10/05)
TITLE: Why Me? | Previous Challenge Entry
By Dean Herring
There's no way I'll be the last,
But sometimes life seems so unfair,
And patience is surpassed,
And tho' it's not original
That's not the point you see,
I want to know a simple thing,
Tell me God, WHY ME?
Adam said it, so did Eve,
And Cain and Abel too,
Noah said it 3 days in,
To floating with his zoo,
Abraham was overheard
Jacob was quite clear,
Joseph kept repeating it,
But Moses hardly dared.
Joshua was about to
But then the walls came down,
Samson finally got it,
And brought the temple down,
Gideon was sheepish
So checked it with a fleece,
Samuel, Saul and David,
Each said it once at least.
Elijah said it 'cos he thought,
He'd been left high and dry,
Jonah wished that he had,
And still it was his cry,
Daniel surely whispered it,
As lions eyes lit up,
Jeremiah cried it out,
Lamenting the corrupt.
Zaccheus, he said it,
From way up in his tree,
Lazarus mumbled something,
I'll bet it was 'Why me?'
Peter said it as he sank,
Much to his surprise,
And even Jesus said it,
Just before he died.
So I guess I'm not original,
I s'pose I'm not the first,
God hasn't left me here to rot,
And maybe I'm not cursed,
I'll put my hand in Gods hand,
Close my eyes and grit my teeth,
Then see it as a challenge,
I guess it's called belief.
©Dean Herring 2005
The opinions expressed by authors may not necessarily reflect the opinion of FaithWriters.com.
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text:
hi there! got question again.so if i had bleeding last july 28 and took birth control pills (cos i thought it was my period).is it safe to say that im protected to any sexual intercourse that happened when i had the bcp?what if i missed taking the pill for a couple of hours?i wasnt able to take it on time. btw i had again another pregnancy test and it says negative.August 15, 2011 - 9:40am
Date of Menstruation:
May 2 [for the month of May],May 31 [for the month of June],July 3 [for the month of July]
I had sex last July 18 which is the 16th day from the start of my period.he used condom when he finally entered but im not 100% if he used it properly.but i saw a cum in it before he pulled out.i was just wondering if theres a possibility of leakage.7hrs after the act [10.30pm], I took an emergency contraceptive pill which is nordette 4pcs. Then took it again 12 hours after [10.30 am the next day], 4pcs and 12hours after - 4pcs [10.30pm same day].july 28 i had a very heavy bleeding which lasted for approx 2 days.i am expecting that this is my period bec i am expecting my period to come Aug 3 but it came few days earlier.i am taking birth control pills right now which will end on aug 17. so i have 7 days to wait for my period to come.i took the pregnancy test awhile ago and it says negative (is this accurate?).im still worried if i can still be pregnant?and when should i expect my period to come?please enlighten me.thanks
Add a Comment5 Comments
hi rosa,
thank you for the immediate response. i took the pregnancy test awhile ago and it says negative. i was just wondering if i can say that the result is accurate? that i am not pregnant?
Hi Anon,
If your period was due on August 3rd, any pregnancy test taken after that date is over 99% accurate. So, that would make you not pregnant.
RosaAugust 12, 2011 - 11:19am
Hi Anon,
When using a condom, you can tell if it has torn by looking at it when it is taken out and gently squeezing the bottom-- if sperm leaks, then the condom has torn. Usually condoms do not leak from the top unless it is very large for the penis size in which case it probably wouldn't even stay on.
Side effects of Nordette include: nausea, distention, breast tenderness, altered menstruation cycles, and breakthrough bleeding. There are chances of some women experiencing serious side effects like sudden weakness, chest pain, migraine headaches, heavy spotting, and elevated cholesterol.
Your period for august may be irregular due to the two days of heavy bleeding you had on the 28th. Please try not to stress out over this, your chances of conceiving were very low to begin with and by taking the oral contraceptive you should have eliminated the possibility.
You can take a pregnancy test at this point if you would like some peace of mind.
All the best,
RosaAugust 11, 2011 - 9:24am
what i meant was i saw a cum after the deed,the time he pulled out.August 11, 2011 - 6:37am
All user-generated information on this site is the opinion of its author only and is not a substitute for medical advice or treatment for any medical conditions. Members and guests are responsible for their own posts and the potential consequences of those posts detailed in our Terms of Service.


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text:
Tarot Card Meanings
Ten of Spades
Protect yourself while the storm rages and focus on rebuilding after it passes.
Nine of Spades
Page of Spades
Watch Now: Ten of Spades Video
Learn the meaning of the Ten of Spades Tarot card in under a minute!
General Meaning
The Ten of this suit represents finality, the end of something. As is easy to grasp from the picture in many decks, there is no hope for revival here. A limit has been reached, a line has been crossed and there is no turning back. In some situations this may be felt as a tragic loss, but it often brings with it a paradoxical sense of release and closure. The waiting and wondering are over. There is no more ambiguity. You can rightly let go and move on, as there is no more progress to be made here.
Emotionally and psychologically, this card appears when one is exhausted and used up, burnt out by the effort of caring and responding and trying to make a difference. When a person feels this way, they have reached burnout and can no longer be held responsible for anything, and therefore can be forgiven for caving in or ceding the fight. The simple instructions are: "Go no further along these lines!"
In the Reversed Position
The Ten of Swords is reversed suggests that you may have been over-dramatizing your predicament. In an attempt to get sympathy and understanding from others, you have begun to believe your own stories and excuses.
Ask yourself what you would do if there were no one around to sympathize with you. Would you be making a faster recovery? If you reframe your situation to emphasize the chance to start fresh, things won't seem so bad. Consider the possibility that you are indulging in drama; take a more accurate look at your situation.
In the Advice Positon
The Ten of Swords in this position advises that you lay low for a while. Don't make a move. Keep yourself as safe as possible until the drama, even the possible trauma, plays itself out. Once the turmoil dies down, then you can assess the damage and start to make repairs. The situation can be compared to a hurricane moving through the neighborhood. You can't be sure whether it is going to pass over a corner of the field or whether it is going to slam into your house.
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SWORDSAce of Spades
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text:
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The Average Salary of a Request for Proposal Specialist
A request for proposal specialist helps a company bring in new business. These specialists respond to requests for proposals and information from current and potential clients. A major job duty is completing the questionnaires sent by clients by the due date. This typically includes a great deal of collaboration with others to ensure responses are thorough and accurate.
RFP Specialist Salary
In 2010, the United States Department of Labor cited the average annual salary for technical writers, including an RFP specialist, as $63,280. The average starting salary for the position was $37,160.
Regional Comparisons
According to Simply Hired, the average annual salary for an RFP specialist is $75,000 in Washington D.C., $62,000 in Los Angeles, $54,000 in Miami, $57,000 in Dallas and $68,000 in Boston. The Department of Labor states that top-paying states for technical writers in general are Washington, California, Massachusetts, Connecticut and Virginia. The discrepancy is due to the high cost of living in those states.
Contributing Factors
RFP specialists work for companies including electric power generation, transmission and distribution; software publishers; wholesale electronic markets, agents and brokers; railroad rolling stock manufacturing; and communications equipment manufacturing. The amount of time an RFP specialist has worked in the field has an impact on annual salary, and those with five years of experience or more tend to earn a higher salary than those with less experience.
Industries with Greatest Amount of Workers
Computer system design, consulting, architectural and engineering, employment services and software companies typically have the highest concentration of RFP specialists. Advertising agencies and financial services companies are also major employers for professionals working in the field.
Career Outlook
The Department of Labor predicts the employment of technical writers will increase by 17 percent through 2020. Increased developments in scientific and technical products and online support will boost the need for this position. Full-time RFP specialists may receive increased competition from freelancers working in the field, however.
2018 Salary Information for Technical Writers
Technical writers earned a median annual salary of $71,850 in May 2018, according to the U.S. Bureau of Labor Statistics. On the low end, technical writers earned a 10th percentile salary of $53,990, meaning 90 percent earned more than this amount. The 90th percentile salary is $89,730, meaning 10 percent earn more. In 2018, 55,700 people were employed in the U.S. as technical writers.
- United States Department of Labor: Technical Writers
- United States Department of Labor: Occupational Employment and Wages, May 2012, Technical Writers
- StartUp Hire: RFP Specialist
- Campus Explorer: How Much Will an MBA Increase My Salary?
- Simply Hired: Average RFP Specialist Salaries in Washington D.C.
- Simply Hired: Average RFP Specialist Salaries in Los Angeles
- Simply Hired: Average RFP Specialist Salaries in Miami, FL
- Simply Hired: Average RFP Specialist Salaries in Dallas, TX
- Simply Hired: Average RFP Specialist Salaries in Boston, MA
- U.S. Bureau of Labor Statistics: Occupational Outlook Handbook: Technical Writers
- Career Trend: Technical Writers
Photo Credits
- Digital Vision./Photodisc/Getty Images


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Date(s) - September 28, 2020 - September 30, 2020
All Day
Categories No Categories
Greetings from the Christian Schools Canada Leadership Conference 2020 Planning Committee!
We look forward to welcoming you to Vancouver for the 10th Christian Schools Canada leadership Conference at the Marriott Pinnacle, Vancouver from Monday, September 28 – Wednesday, September 30. Deeper Learning Conference to be held on September 28.
We are especially pleased to spend time with the theme of Exclusion and Embrace, featuring plenary speaker: Miroslav Volf, professor of Theology and Director of the Yale Center for Faith and Culture at Yale University.
Our prayer is that this conference will inspire, challenge and strengthen you in your personal and communal leadership journey. We encourage you to use this unique opportunity also to build relationships and network with Christian school leaders from across Canada and from different countries around the globe. We're looking forward to seeing you in beautiful Vancouver, BC!
Twitter.com/ChristianSchCan #csc2020conf


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Aqua Ionizer Deluxe 5.0 is one of the most compact Water Ionizers on the market. This water ionizer also functions as a beauty machine and has preset settings for balancing skin and hair pH (acidic settings) as well as daily drinking for detoxification, better hydration and general health.
Ionized alkaline water creates natural antioxidants (reduced ORP levels) and supports better hydration along with other health benefits. This attractive and quality-built unit from Air Water Life comes equipped with everything you need for quick and easy installation to your kitchen, bathroom, or utility room faucet. It is both stylish and intuitive to use, installs in just minutes with an easy to follow diagram, instructions and an owner's manual. You will be producing clean, vitalized, antioxidant rich alkaline drinking water within minutes of set up.
Having the Aqua Ionizer Deluxe 5.0 reduces plastic waste and the need to purchase bottled drinking water providing delicious fresh antioxidant rich water. The Aqua Ionizer Deluxe is our most advanced and powerful water ionizer. Depending on source water, the Aqua Ionizer Deluxe 5.0 produces water with a pH of between 5.0 to 11.0 alkaline. It also boosts the waters age fighting antioxidant potential with a -600 to +600 ORP. Each inner activated carbon filter cleans and treats 4,000 liters.
7 Healthy Water Settings
Produces pH 5.0 to 11 Alkaline Water
Antioxidant potential -600 to +600 ORP
Alkaline Water is high in antioxidants and ideal for healthy drinking water. Acidic Water though not intended for drinking, is perfectly suited for skin, hair and scalp conditioning as well as plant care.
In addition to allowing you to adjust your water's pH level, all our water ionizers have built-in Active Carbon Water Filters designed to remove a whole host of harmful pollutants found in tap water.
The powerful water ionizer benefits come from re-hydrating parched cells and re-balancing the bodies' pH. These amazing Antioxidant properties are only found in Ionized Alkaline Water.
HYDRATE – Increase Your Ability to Stay Hydrated
ENERGIZE – Boosts Your Energy Levels
FIGHTS FREE RADICALS – Protect Your Healthy Cells
DETOXIFY – Aid in Elimination of Toxins
LOWERS BODY ACIDITY – Balance Your Bodies pH Levels
REVITALIZE – Replenishes Vital Minerals and Electrolytes
Aids in Detoxification while the healing benefits of certain types of water are not yet fully understood, your body when properly hydrated has the ability to heal itself of many ailments.
Your body's ability to heal itself is far greater than anyone has lead you to believe. When you aid in your bodies elimination of toxins you clear the way for healing to occur. With the Aqua Ionizer Deluxe water ionizers, you can make your own detox water beverages which taste amazing and are healthy too!
Shopping online should be enjoyable and we want you to love your experience with us! If you have any problems with your order or aren't satisfied with your product, just let us know and we'll get you another product in the mail or your money back! We will do WHATEVER it takes to make sure you are 100% satisfied with a risk-free 30 day guarantee.
We have 24/7/365 Email Support. Please contact us if you need assistance.
Shipping Info
All Shipments are trackable through USPS tracking (or your local mail carrier). Due to high demand, please allow 3-7 business days for delivery, applies to high volume orders.
Refund Policy
We accept returns within 30 days from date of delivery of the item only in the case of damaged, defective or wrong products received.


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The Deer Salami is produced in the region of Andalusia and it is an exceptional charcuterie product. It has a rich and succulent taste where you can appreciate very well the taste of bush meat.
Our Salami is made of the best bush meat using craft methods. The Deer Salami is presented in whole piece and air dried. By cutting it you can appreciate the dark red colour and the white veins typical for this kind of sausage. The Salami has a characteristic taste of bush meat, a light touch of pepper and a subtle spiciness.
Enjoy with our Deer Salami a high quality charcuterie product. Try it as snack, together with a cup of wine or in a sandwich.
aprox. 190 gr.
whole piece
vacuum packed
The Deer Salami is a high quality charcuterie product from the Region of Andalusia. The characteristic taste of bush meat, the perfect point of air drying and the subtle spiciness convert this salami in an extraordinary product.
aprox. 190 gr.
whole piece
vacuum packed


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Yelvertainment has been awarded £1,364.16 from Northamptonshire Community Foundation through the Constance Travis Endowment Fund
Yelvertainment aims to raise the social and community wellbeing of Yelvertoft and the surrounding area through music and entertainment. The group provides a programme of events and activities, giving opportunities for members of the local community to take part.
Funding from the Foundation will help Yelvertainment to provide equipment to a number of community groups in the area, enabling them to both carry out existing activities more effectively and to extend the range of activities they offer.
Alan Saunders, Chairman of Yelvertainment, said: "Yelvertainment is proud to accept its grant award through the Constance Travis Endowment Fund. Thanks to Northamptonshire Community Foundation for its assistance in obtaining it. The grant will benefit the community and:
Ensure the survival of the Yoga classes which have grown to be a popular activity targeted at villagers and, in particular, older people.
Improve the catering quality of the Hub in the Pub get-togethers and Yelvertainment events for all villagers thus helping their continuing success.
Improve the quality of the revived annual Wassail celebration which is designed to promote the environment and conservations work of Yelvertoft Pocket Park."


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Our experienced and friendly team at Plumbing For Life can assist you with all your gas requirements. The installation of gas services is a potentially dangerous situation and such work should only ever be carried out by a licenced, professional gas fitter. Our plumbing technicians are licenced and experienced and will find solutions to all your gas requirements.
We can assist you with getting gas installed on your property, fit the gas meter and assist you with gas certifications. We can advise you on the selection of the most up to date gas appliances and expertly perform all your gas fitting and appliance installations.
Our licenced gas fitters can install gas bayonets for you to be able to use reliable gas heating for all your BBQ'ing needs. You'll never have to 'swap and go' again! Gas heating can also provide your house with warmth on those cold winter nights and greatly reduce those spiraling power bills.
Call Plumbing For Life for all your gas fitting needs, including:
For further information regarding any gas fitting concerns you may have, please give Michael from Plumbing For Life a call on 0414 629 082. We are fully insured and our workmanship is guaranteed!


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Saudi Arabia's Aid Agency Takes Initiative to Boost Rehab Projects
On Wednesday, the King Salman Humanitarian Aid and Relief Center (KSRelief) coordinated the first "Developing the rehabilitation project of recruited and war-affected children in Yemen" workshop at King Saud University in Riyadh.
Dr. Abdullah Al-Rabeeah, supervisor general of KSRelief, stated that the center has assured to arrange a string of workshops directed at enhancing the quality of relief projects and programs conforming to the best practices and underscoring the crucial role of the Kingdom in the international humanitarian work.
He stated that the dream of KSRelief solicits to make the center an international pioneering center to spread these values all over the world, where the Kingdom plays a guiding role in reducing the suffering of all people around the globe so that they get a chance to lead a decent life, based on the principles of forbearance, love and peace.
The center was chosen by the UN as a member to form an international team to collaborate in restoring children. The team comprises of 16 specialists who have enough experience to tackcle the matter of recruited children.
Al-Rabeeah stated that the significance of this project at the international humanitarian level has led us to commune with specialists, consultants and pertinent people in Saudi Arabia and Yemen. He stated that he strongly thinks that their outlook would evolve and improve the project with a sustainable scientific approach, based on structuring and emancipating scope in Yemen to become an international humanitarian role model.
There is no check to the Saudi capabilities, where they come from the Kingdom's moral dedication to back humans and societies going through crisis situations and give them a decent life, especially to women and children who are considered among the most severely affected groups by disasters and conflicts.
The launch of the workshop was attended by Yemen's Minister of Human Rights Mohammed Askar, officials from the King Saud University, and academic experts and specialists in the humanitarian field.
Article Tags: Saudi Arabia · Rehab Projects


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Appian World 2019 for Financial Services
20 Years Later—And We're Still Going Strong!
Wow—less than a month to go before Appian World 2019. It's Appian's 20th birthday! All four of the original founders—who were there 20 years ago when the company was born—will be there for this great celebration. In 1999, they had a belief that talented and passionate people, given the power to be heard and the autonomy to excel, could deliver amazing results. Now, 20 years later in beautiful San Diego, you have the opportunity to witness those amazing results. You won't want to miss these three fabulous days where you can network with peers in financial services and learn how Appian's low-code platform is helping them improve customer experience, achieve operational excellence, and simplify global risk management and compliance.
World-Class Speakers
In addition to some of the world's most prominent leaders in financial services, there will be many other phenomenal speakers talking about our common, ongoing journey—the journey of change. I am particularly looking forward to the session, "Leading Through Change," with panelists from a diverse group of organizations, including Black Girls CODE and Goldman Sachs. Andre Agassi is another great speaker I'm looking forward to. I cannot wait to hear the tennis legend talk about perseverance and determination.
Join Us May 13-15 at the Hilton San Diego
At Appian World 2019, you can arrange one-on-one sessions with Appian experts and participate in hands-on product training. You'll learn about new technologies like Artificial Intelligence (AI), Machine Learning (ML), and Robotic Process Automation (RPA)—all while connecting with industry peers who are facing similar challenges.
Some highlights of the week include:
Monday, May 13
- Hands-on labs, training, jumpstart sessions
- Product expert meetings: Request a meeting today
- Welcome reception: Enjoy delicious food and fun music while networking with your peers
Tuesday, May 14
- Appian World 5k: All proceeds will be donated to Black Girls Code
- Optimizing Efficiency and Customer Experience in Financial Services: Vanguard
- Developer Jam
- Rethinking Automation for the 21st Century: Aegon Nederland & Capital Group Companies
- Robot and Human, Hand in Hand: Unione Fiduciaria and KPMG
- Conference party aboard the USS Midway: Docent Tour
Wednesday, May 15
- Janus Henderson: Simplifying Capital Markets Operations and Service Delivery with Appian
- Hackathon
- Closing keynote featuring Andre Agassi
Thank you to all our sponsors who helped make this such a great event. I look forward to seeing you there! Please register today at appianworld.com.


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Monthly Archives: October 2012
spaghetti squash, where have you been all my life?
i have had "try spaghetti squash" on my to-do list for at least 3 years, but for some reason have never gotten around to it. UNTIL NOW. and man, have i been missing out! this stuff is awesome! even the … Continue reading
because i needed another scarf
really, i swear (*cough*). ok, so i didn't, but i started this scarf last winter and then abandoned it for months, so i thought it was time to finish it off. there was just enough yarn left to make a … Continue reading
mission accomplished
significant progress has been made since my last post! i ended up calling the shawl finished after one more row, because it was getting really large. wove in the ends, and voila! a shawl to keep me warm on chilly … Continue reading
it never fails
when i am 90% finished with a crochet project, i become obsessed with starting something new. this leads to a whole lot of wip's and not a lot of finished products. i am currently working on a big shawl to … Continue reading
happy spooktoberween!
so, the fella and i love us some halloween. this year, we went to a party at the home of some friends from college. they host a party each year with a different decade as the theme. this year was … Continue reading


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Avengers: Endgame TV Spot – Found (2019) | Movieclips Coming Soon SUBSCRIBE: https://youtu.be/nE2uhv-ZCSw : Check out the new TV Spot for Avengers: Endgame starring Scarlett Johansson! Let us know what you think in the comments below.
► Buy Tickets to Avengers: Endgame: https://www.fandango.com/avengers-endgame-2019-215871/movie-overview?cmp=CSYT_YouTube_Desc
Want to be notified of all the latest clips and behind the scenes footage? Subscribe to the channel and click the bell icon to stay up to date.
US Release Date: April 26, 2019
Starring: Robert Downey Jr., Brie Larson, Scarlett Johansson
Directed By: Anthony Russo, Joe Russo
Synopsis: After the devastating events of Avengers: Infinity War (2018), the universe is in ruins. With the help of remaining allies, the Avengers assemble once more in order to undo Thanos' actions and restore order to the universe.
Watch More:
► Coming Soon to Theaters: http://bit.ly/2mkFBqr
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The Fandango MOVIECLIPS COMING SOON channel is home to all the movie clips, trailers, behind the scenes videos, and more from the best upcoming movies. Subscribe to learn more about the movies you love!


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Senior Adviser
Cecilie Wathne leads U4's thematic work on Measurement and Evaluation. Prior to joining CMI, she was head of planning, monitoring, evaluation, accountability and learning (PMEAL) at the Norwegian NGO Strømme Foundation. She has also worked as a researcher at the Overseas Development Institute on issues related to aid effectiveness and accountability. Cecilie holds a MSc in Economics for Development from the University of Oxford and a BSc in Economics, a BA in Political Science and a Certificate in Quantitative Methods from the University of Washington. She has undertaken fieldwork in over a dozen countries in Asia and Africa.


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Vault Watch
This product is sold outSize Guide
As Vault's combination lock revolves, they remind you that every second is priceless. Vault is unusual in that it tells the time in the 3 o'clock position–further punctuated by the setting crown. Because of this unique arrangement, we're asked to consider the seconds first, then the minutes, then the hour–helping to further put time in perspective.
Vault features a lugless case which continues to the round theme of the lock…
The Vault Time Machine Watch is available with a brushed stainless steel case. It measures 40mm (1.57?) in diameter and has a 20mm wide black leather band. It is constructed with three discs that turn clockwise, not unlike the mechanism of a vault and markings on the underside of the glass lens that "mark time". The watch measures 9 1/2? from end to end with 7? to the closest hole and 9 1/2? inches from the farthest hole. The brushed stainless steel case is complemented with a brushed stainless steel buckle.
Join us for First Friday & other upcoming gallery events


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The Animal Welfare Committee has the authority, on behalf of the University to:
- Ensure that any objectionable procedures be stopped if, in consultation with the veterinarian, the Animal Welfare Committee considers that unnecessary pain and distress is being experienced by the animal.
- Ensure that any use of animals be stopped which, by deviating from the approved protocol, causes the animals to experience undue pain and distress.
- Ensure that an animal be humanely killed if, in consultation with the veterinarian, the Animal Welfare Committee feels that pain or distress caused to the animal cannot be alleviated.
AWC Terms of Reference
|2 Community Representatives
|Katie Johnston
|Tad Mitsui
|1 Faculty Member from a Department other than those using animals
|Ian McAdam (English)
|01-09-2015 to 31-08-2019
|1 Representative from Occupational Health & Safety
|Lorna Selinger
|1 Veterinarian
|Isabelle Gauthier (ex officio)
|3 Faculty members experienced in research animal care and use, from Neuroscience, Biological Sciences, and Psychology
|Greg Pyle (Biological Sciences)
|01-01-2018 to 31-08-2020
|Bryan Kolb (Neuroscience) - Chair
|01-01-2019 to 31-12-2021
|Jean-Baptiste Leca (Psychology)
|01-05-2019 to 01-05-2021
|A student representative
|vacant (Biological Sciences)
|vacant (Neuroscience)
|Francisco Gomez (Psychology)
|01-01-2018 to 12-31-2019
|A technical staff representative
|Karen Dow-Cazal, Animal Care Technician, CCBN (ex officio)
|Eric Stock, Aquatic Research Facility Coordinator, ARF (ex officio)
|Research Services
|J. Keiko McCreary, AWC Coordinator (ex officio)
Membership Terms:
The University Animal Welfare Committee (AWC) includes representation from the following areas:
- Three faculty members, experienced in research animal care and use, from departments conducting research with animals. Faculty members should be from Neuroscience, Biological Sciences and Psychology.
- A faculty member from a Department other than those using animals.
- A representative from Safety Services.
- A veterinarian, experienced in the use of animals for research (ex officio).
- A non-University member representing the community interests and concerns.
- A technical staff representative from the animal research facilities.
- A student representative.
- The AWC Coordinator (ex officio)
- Other individuals may be asked to review the protocols as the need arises. A Chair is elected annually. The Chair should not be an ex officio member, nor be directly involved in the management of the institutional animal facilities, nor be a clinical veterinarian for the institution, nor be an animal health or veterinary personnel member charged with ensuring compliance with CCAC guidelines, nor be involved in the preparation of a significant number of the protocols to be reviewed by the Committee, in order to avoid potential conflicts of interest.
- Committee members are appointed by the Vice President, Research for two-year periods, with terms staggered to provide continuity. Normally, terms may be renewed once.
Normally, two year alternating terms. The Committee reports through the Vice President, Research to the President.
Misc. Information:
Quorum = 50%+1 (must include the Chair, Veterinarian, a Community Representative and the AWC Coordinator)
Canadian Council on Animal Care


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Texas A&M coach Jimbo Fisher: 'This game was truly won in the trenches'
COLLEGE STATION — Texas A&M offensive linemen's visits with the media this season have been brief but memorable.
In September, left guard Jared Hocker guaranteed a victory at Clemson. It didn't pan out, but Aggies applauded his bravado. On Saturday center Colton Prater, in his first meeting with reporters this year, explained why he believed A&M was strengthening as the season has pressed on.
"That's one thing this team has been good about — we always beat people down," Prater said following the Aggies' 30-6 beatdown of South Carolina at Kyle Field. "When it comes down to these tough, hard-fought games, it's always them rolling over, not us."
Prater's bluster was based on A&M controlling the ball a season-high 41:39 of the game's 60 minutes. That was more than twice as long as South Carolina (18:21). The Gamecocks never reached the end zone, and Prater said the Gamecocks' defense was gassed from the extended time on the field.
"The (South Carolina) defense was out there complaining about it, they were feeling it, too," he said. "That's fun for an offensive lineman, to hold the ball that long, beat people down and put your will on them."
A&M (7-3, 4-2) held the Gamecocks (4-7, 3-5) and besieged coach Will Muschamp to their lowest point total in the six-year history of the series, all A&M victories, and South Carolina didn't convert a third down until about nine minutes remained.
"The game was truly won in the trenches," A&M second-year coach Jimbo Fisher said. "Our defensive play was outstanding … to hold them to 45 yards rushing."
The Aggies now go on the road for their final two contests, at No. 4 Georgia on Saturday and at No. 1 LSU on Nov. 30.
Despite the final score, the nearly 105,000 fans on hand soaked in a defensive battle through three quarters, and the teams played a scoreless third quarter before the Aggies' Seth Small booted a 40-yard field goal early in the fourth to give A&M some breathing room with a 16-3 lead.
"This was a huge performance for us defensively," A&M linebacker Anthony Hines said. "This give us confidence, not only in getting a win but how well we played."
South Carolina's final score was set up by an A&M fumble on its own 12-yard line. The resulting Gamecocks drive even went backward nine yards before a final field goal late in the fourth quarter.
"They affected the quarterback," Fisher said of A&M's persistent pressure on freshman Ryan Hilinski. "The throws came out early … the (defense) played very intelligently. Toughness and intelligence are key."
Cordarrian Richardson, who had a 75-yard touchdown run midway through the fourth quarter to put away the win, rushed for 130 yards on six carries, and Isaiah Spiller finished with 129 yards on 24 carries, marking the first time this season against SEC competition the Aggies had two runners with at least 100 yards.
The Gamecocks sacked Mond twice and popped him on multiple occasions, A&M's biggest concern heading into Athens, Georgia, and Baton Rouge, Louisiana.
"Kellen did a good job of managing the game, doing the right things again and again," Fisher said. "He took too many shots in the game, and we've got to clean that up. We're going to need to (play better) with the next two games coming up."
The Aggies have won four consecutive games for the first time since closing out last season with a four-game win streak. The Aggies started this season trading wins and losses over their first six games.
"Here's what I like most — we're practicing better," Fisher said of the Aggies getting better as the season winds down.
A&M and Georgia haven't played in a decade, a 44-20 Bulldogs victory in the 2009 Independence Bowl. The Aggies last played in Athens in 1980, a 42-0 Georgia win by the eventual national champions.
Once the Aggies get their first visit to Georgia in nearly four decades behind them, they'll face a top-ranked LSU squad bent on revenge following the Aggies' seven-overtime victory a year ago at Kyle Field.
"We don't want to look at any game ahead, and we were able to close out (this season) at Kyle Field big-time against an SEC team," Mond said. "We're 4-0 in the last four, and now we're getting ready for No. 4 in the country and No. 1 in the country."


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Good morning.
1. Markets will be a big deal today, because it's Rates Day for the RBA. No one – except maybe TD Securities – is considering a hawkish RBA today. Here's your 10-second guide. But whichever way the decision falls, it's likely to be a big day for the Aussie dollar. The ASX200 is likely to open higher, with futures traders predicting that it will bounce back from yesterday's fall with a solid global lead.
2. New Jersey governor Chris Christie makes Trump look like Trump Lite. He's the guy whose staff members engineered a four-hour traffic jam by closing toll lanes on a bridge out of Fort Lee, around the time Fort Lee's mayor was refusing to nominate Christie in the 2013 New Jersey gubernatorial election. Today, he's on front pages everywhere enjoying a day at the beach – on a day when beaches were closed to the public because of a government shutdown he ordered. When asked why he was enjoying the beach when the public couldn't, Christie said:
"I'm sorry they're not the governor."
3. People Looking at Trump Funny is the new bloodsport for media photographers in 2017. You're a lay-down for a viral if you can pick something like this out your 1000 shots for the day:
That's space legend Buzz Aldrin at the signing of an executive order to revive the National Space Council. But the real action was taking place on Buzz's wrist and ankles:
4. The Turnbull government has opened its doors again to overseas tech workers. Immigration minister Peter Dutton just promoted another 36 jobs from the two-year to the four-year scheme which can provide a path to permanent residency.
5. Elsewhere in IT Worker World, there's an ethics debate raging about whether it's cool for programmers to automate their work so they can take most of the rest of the week off. It was sparked by one anonymous post on Stack Overflow by a remote worker who says he has used scripts to cram his whole job into two hours of work a week and hasn't told his boss.
6. Your Medicare details are for sale. For just $30 in Bitcoin, a hacker is selling the details of "any Australian citizen". The Guardian can confirm it's true, because it bought the accurate details of a staff member.
7. Bryan Cranston hasn't moved to Canada. Stephen King hasn't moved to Canada. Lena Dunham hasn't moved to Canada. In fact, twice as many people moved to Canada in 2012 after Obama's second win than they did after Trump's win. And the data suggests a trend of declining applications overall.
8. The stats of a winner:
Final Punch Stats: Jeff Horn def. Manny Pacquiao by Unan. Dec. Pacquiao out landed Horn, 182-92. Horn landed just 15% of his total punches. pic.twitter.com/dImHXkowyO
— CompuBox (@CompuBox) July 2, 2017
While Manny Pacquiao took home about $US10 million from his loss to Jeff Horn on Sunday, Horn would have struggled to recoup a tenth of that, even with ticket incentives. He's likely to be looking at $2 million for a rematch with Pacquiao in November. But right now, the nature of his ugly, unlikely but inspiring win has pundits thinking twice about whether Conor McGregor might actually be able to hand Floyd Mayweather the first loss of his career.
9. On the opening night of Wimbledon, Nick Kyrgios only lasted two sets before retiring injured. And John Millman only lasted three sets against Rafael Nadal while being soundly thrashed.
10. We're 12 days away from the start of the penultimate season of Game of Thrones. And while fans may be disappointed to have learnt it's only six episodes long, it was revealed overnight that the final episode of each season will be feature-film length. And possibly every episode of the final season. That's seven Game of Thrones movies.
BONUS ITEM: "Stop talking over the top of-", "I AM NOT TALKING OVER THE TOP OF YOU."
Have a great day.
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