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text:
I was wondering if there is any R package capable to do multilevel factor analysis?
Take the 2-minute tour ×
Stack Overflow is a question and answer site for professional and enthusiast programmers. It's 100% free, no registration required.
check out http://openmx.psyc.virginia.edu/. The original MX software (http://www.vcu.edu/mx/) can do multilevel factor analyis (see e.g. [1]), so I suppose
[1]: Bai, Yun and Poon, Wai-Yin(2009)'Using Mx to Analyze Cross-Level Effects in Two-Level Structural Equation Models',Structural Equation Modeling: A Multidisciplinary Journal,16:1,163 — 178

subset: commoncrawl


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Page 1
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The Journal of Physical Chemistry B is published by the American Chemical Society.
1155 Sixteenth Street N.W., Washington, DC 20036
Effect of the Barometric Phase Transition of a DMPA Bilayer on the Lipid/
Water Interface. An Atomistic Description by Molecular Dynamics Simulation
J. J. Giner Casares, L. Camacho, M. T. Martn Romero, and J. J. Lpez Cascales
J. Phys. Chem. B, 2007, 111 (49), 13726-13733 • DOI: 10.1021/jp075948v
Downloaded from http://pubs.acs.org on February 9, 2009
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Effect of the Barometric Phase Transition of a DMPA Bilayer on the Lipid/Water Interface.
An Atomistic Description by Molecular Dynamics Simulation
J. J. Giner Casares,†L. Camacho,†M. T. Martı ´n Romero,†and J. J. Lo ´pez Cascales*,‡
Departmento Quı ´mica Fı ´sica y Termodina ´mica Aplicada, Ed. Marie Curie, Campus de Rabanales, UniVersidad
de Co ´rdoba, 14014 Co ´rdoba, Spain, and UniVersidad Polite ´cnica de Cartagena, Centro de Electroquı ´mica y
Materiales Inteligentes (CEMI), Aulario II, Campus de Alfonso XIII, 30203 Cartagena, Murcia, Spain
ReceiVed: July 27, 2007; In Final Form: September 24, 2007
Understanding the structure and dynamics of phospholipid bilayers is of fundamental relevance in biophysics,
biochemistry, and chemical physics. Lipid Langmuir monolayers are used as a model of lipid bilayers, because
they are much more easily studied experimentally, although some authors question the validity of this model.
With the aim of throwing light on this debate, we used molecular dynamics simulations to obtain an atomistic
description of a membrane of dimyristoylphosphatidic acid under different surface pressures. Our results
show that at low surface pressure the interdigitation between opposite lipids (that is, back-to-back interactions)
controls the system structure. In this setting and due to the absence of this effect in the Langmuir monolayers,
the behavior between these two systems differs considerably. However, when the surface pressure increases
the lipid interdigitation diminishes and so monolayer and bilayer behavior converges. In this work, four
computer simulations were carried out, subjecting the phospholipids to lateral pressures ranging from 0.17 to
40 mN/m. The phospholipids were studied in their charged state because this approach is closer to the
experimental situation. Special attention was paid to validating our simulation results by comparison with
available experimental data, therebeing in general excellent agreement between experimental and simulation
data. In addition, the properties of the lipid/solution interface associated with the lipid barometric phase transition
were studied.
1. Introduction
This work was focused on the venerable and still prominent
topic of lipid bilayer structure, which plays a crucial role
controlling the diffusion of nutrients, ions, and water between
the inner and outer cell together, while providing a suitable
environment to other molecules embedded in the membrane,
such as membrane proteins or cholesterol, among others.1,2
Understanding the atomic interactions that control the behavior
of this complicated system is of unquestionable importance. One
of the aspects that has attracted increasing interest during recent
decades is the polymorphic phase transition of lipid bilayers
associated with temperature due to the importance of this process
from a biological viewpoint. In this respect, a temperature
denoted transition temperature (Tm) defines the temperature at
which lipids change from a gel (P′?) to a liquid crystalline state
(LR) at a constant external pressure.3,4
However, polymorphic phase transition may also be associ-
ated with different states induced by an external pressures or
barometric transition.5,6To understand the atomic interactions
that govern these phase transitions induced by an external
pressure, Langmuir air-water lipid monolayers have been
widely studied,7mainly because they enable the surface pressure
on the lipids that form the monolayer to be studied, and also
because a monolayer is basically half of a symmetric lipid
bilayer. For this purpose, different experimental techniques have
been used, such as scattering techniques, X-ray and neutron
reflectivity measurements, infrared reflection-absorption spec-
trometry (IR), NMR studies, and the ellipsometric technique.7-13
In the case of monolayers, different barometric phases have been
denoted, such as G (gaseous), LE (liquid expanded), LC (liquid
condensed), and SC (solid condensed).7
Improvements made in simulation algorithm and the increases
in the computing power attained during recent years have led
to molecular dynamics simulation14emerging as a powerful and
precise technique that provides atomic insight into the dynamic
Keeping this in mind with the aim of obtaining an atomic insight
into the interactions that control the phase transition of a lipid
bilayer, a computer simulation of a lipid bilayer of dimyris-
toylphosphatidic acid (DMPA-) was carried out. The main
reasons why we chose this type of lipid instead of DPPC or
DPPE were the following: first, because charged lipids at
physiological conditions play a crucial role in the structure and
functions of cellular membranes introducing an asymmetry
between the inner and outer side of the membrane, which can
constitute up to 20% of the total lipids, and second, because
DMPA-is a lipid widely used in the study of lipid monolay-
ers.20,21,9,11,13,22In this setting, considering that electrostatic
interactions control the lipid-lipid interactions in charged
lipids,16it is expected that different lipid packing associated to
their barometric phase transitions can modify noticeably the
lipid/water interface.
Thus, the main goal of this work was, in a first instance, to
validate the force field of DMPA-, because as far as we know,
this is the first time that this type of lipid has been simulated.
To this end, the properties of the bilayer were studied and
* To whom correspondence should be addressed. E-mail: javier.lopez@
†Universidad de Co ´rdoba.
‡Universidad Polite ´cnica de Cartagena, Centro de Electroquı ´mica y
Materiales Inteligentes (CEMI).
J. Phys. Chem. B 2007, 111, 13726-13733
10.1021/jp075948v CCC: $37.00© 2007 American Chemical Society
Published on Web 11/16/2007
Page 3
compared with available experimental data. After checking and
validating the force field for their liquid crystalline state (LR),
the system was subjected to high-surface pressures, bringing
the bilayer close to liquid condensed state (LC), with the goal
of describing its barometric behavior. Thus, changes in the lipid
structure associated with pressure are described and how these
variations affect the lipid/solvent interface properties is dis-
Assuming that lipid bilayers and monolayers are in fact
different physical systems, our hypothesis is that these systems
behave differently, and we propose an explanation in terms of
lipid-lipid interactions. What we present here is a systematic
and detailed study that supports our hypothesis. The obtained
results suggest that the simulated system behaves like a bilayer
in its liquid crystalline state at low surface pressure but behaves
more like a monolayer in its liquid condensed state when
subjected to high pressure.
For low surface pressures, the most important lipid interaction
is related with lipid tail interdigitation (back-to-back interac-
tions). However, because this kind of interaction is impossible
to achieve in a lipid monolayer, monolayers do not reproduce
bilayer behavior. However, at higher values of surface pressure,
the interdigitation associated with lipid tails vanishes, and the
most important effects are related with lateral interaction
between neighboring lipids. Thus, at sufficiently high pressures,
lipid bilayers and monolayers show the same behavior. In this
way, as has been seen for DPPC bilayers,19lipid bilayers at
high surface pressures reproduce air-water monolayer behavior.
Finally, a detailed study of the lipid/water interface was carried
out for different lipid packing of the membrane.
2. Method and Model
Periodical boundary conditions were considered along the
three-dimensional space, X, Y, and Z. The computer simulation
box was generated by placing two leaflets of 144 DMPA-(i.e.,
288 lipds in total), pointing the lipid heads toward the two water
layers placed on both sides of the computing box. Figure 1
depicts two snapshots of the system at two different surface
pressures. To maintain the electroneutrality of the system, 288
water molecules were substituted by 288 sodium ions (Na+),
so that the whole system was constituted by 288 DMPA-, 288
Na+, and 9780 SPCE water molecules,23which amounted 41436
atoms in total.
Gromacs 3.3. package24,25was used to run the MD simula-
tions, and the generated trajectories were analyzed with a
software code developed by ourselves. A dipalmitoylphosphati-
dylserine (DPPS-) modified force field16was used to carry out
these simulations. The charge distribution of a lipid DMPA-
molecule was determined by CNDO semiempirical method
implemented in HyperChem.26Thus the charge borne by each
atom of the system is depicted in Table 1, corresponding to the
atomic numeration shown in Figure 2. Long range interactions
were modeled by the Lennard-Jones potential with a cutoff of
0.8 nm, and the electrostatic interactions by the Ewald algo-
rithm.27,28All the bonds of the system were constrained by
SHAKE.29The time step used in all the simulations was 2 fs.
Simulations were carried out on a HPC160 parallel computer
using 16 processors with a performance of 1.5 ns of trajectory
length per hour.
In this regard, after setting up the system, the whole system
was subjected to a steepest descent energy minimization process
to remove undesired overlaps between neighboring atoms.
Once the system reached a certain energy minimum, four
simulations were carried out at the same normal pressure of 1
atm (z-axis) and each one to different surface pressures (in the
X-Y plane of the membrane) at 1, 6, 60, and 225 atm (which
correspond to 0.17, 1, 10, and 40 mN/m, respectively). Because
Figure 1. Snapshots of the DMPA-bilayer at two different surface
pressures: (a) 0.17mN/m and (b) 40mN/m.
Figure 2. Atom numeration used in this work for a molecule of
dimyristoylphosphatidic acid, DMPA-.
TABLE 1: Charge Density for Each Atom of Lipid DMPA-
Molecule, Na+Ions, and SPCE Water Molecules, As
Obtained by CNDO Method
atom number atom type
charge (e)
SPCE water
DMPA Bilayer on the Lipid/Water Interface
J. Phys. Chem. B, Vol. 111, No. 49, 2007 13727
Page 4
a thermodynamic normal temperature and pressure (NTP) state
has been simulated, the system was coupled to an external
temperature and pressure bath with temperature coupling
constants, τT, of 0.1 ps and a pressure coupling constant, τP, of
1 ps. In both cases, the Berendsen's algorithm was used.30
To avoid undesired simulation artifacts associated to the
starting conformation of the system, the first simulation at 0.17
mN/m was carriead out at 500 K during 500 ps. The end
conformation of this simulation was the starting point at 0.17
mN/m. Next, the system was cooled down to 350 K, and the
above-mentioned four simulations were carried out for 20 ns
where the end conformation of each trajectory was the starting
point of the next simulation at higher surface pressures. In the
four cases, the temperature of 350 K is above the DMPA-phase
transition temperature Tmof 328 K in a range of pH from 4 to
9.31The first 2 ns of trajectory length were discarded for analysis
purposes because this was the time required to reach the
equilibrium in the four cases.
3. Results and Discussion
3.1. Lipid Area. Figure 3 depicts the area evolution of lipids
as a function of time for several surface pressures. From these
curves, we observe how for a trajectory length above 2 ns that
the system reaches a steady state in the four cases. Thus, the
dimension of the equilibrated computing boxes were: (8.82,
8.68, 7.92), (8.76, 8.72, 8.04), (8.38, 8.26, 8.75), and (7.61, 7.41,
10.27), for the x-, y-, and z-axis of the computing box in nm at
0.17, 1, 10, and 40 mN/m surface pressure, respectively.
The area per DMPA-molecule obtained from our simulation
was compared with experimental data. Note that at low surface
pressure, 0.17 mN/m (or 1 atm), the simulated area was 0.54 (
0.07 nm
obtained by Ziegler et al.,32of 0.58 nm2for its liquid crystalline
state LR. At the same surface pressure, for DMPA-air-water
monolayers, a surface area of 0.8 nm2was measured from the
isotherm surface pressure-lipid area (π-A isotherm) by Lozano
et al.33When surface pressure is increased to 40 mN/m, the
surface area per DMPA-molecule obtained by our simulations
was 0.40 ( 0.07 nm2, which agrees perfectly with the area of
0.4 nm2obtained for monolayer π-A isotherm in its LC state.33
These results are depicted in Figure 4.
The effect of the interdigitation between opposite lipid leaflets
can be observed from the density of methylene groups of lipid
tails of the two lipid leaflets, as shown in Figure 5. Thus, we
observe how an increase in the surface pressure diminishes the
methylene overlap of opposite leaflets, that is, a diminishing in
the lipid-lipid interdigitation.
2, which agrees well with the experimental data
3.2. Lipid-Lipid Lateral Interactions and Lipid Hydra-
tion. The radial distribution function g(r) provides valuable
atomic information concerning neighboring molecules. Thus,
the radial distribution function g(r) is defined as
where N(r) is the number of atoms in a spherical shell at distance
r and thickness δr from a reference atom, and F is the number
density in the computing box.
Hence, the radial distribution function of the atom numbers
4, 11, and 28 (two carbonyl and a phosphate oxygens) around
the phosphorus of a neighboring lipid DMPA-was calculated.
By integrating the first maximum of the radial distribution
function g(r), the coordination numbers of the above-mentioned
atoms (4, 11, and 28) over a phosphorus atom as a function of
the surfaces pressures were calculated and are depicted in Table
Note that an increase in the surface pressure applied produces
an increase in the coordination numbers N(r) between lipids,
which clearly reflects the importance of lateral interactions at
high values of surface pressures. Indeed, the coordination
Figure 3. Lipid running area as a function of time for different surface
Figure 4. DMPA-surface area for different surface pressures: circles
represent the simulated data, squares represent the experimental data
for a DMPA-bilayers, and triangles represent the experimental data
for DMPA-monolayers.
Figure 5. Lipid tail methylene density F in kg/m3along the z-axis for
surface pressures of 0.17 mN/m (a) and 40 mN/m (b). The origin of
the z-axis was placed in the center of the box.
g(r) )
13728 J. Phys. Chem. B, Vol. 111, No. 49, 2007
Casares et al.
Page 5
number for atom 4 (phosphate oxygen) around a phosphorus
atom undergoes a dramatic increase of 73% from 0.17 to
40 mN/m. Less pronounced is the increase in coordination of
the carbonyl oxygens around the phosphorus atom, which is
only around 25% under analogous conditions.
The coordination numbers of water oxygens around atoms 4
and 28 of the lipid molecule (oxygen of phosphate and carbonyl
group, respectively) are depicted with their standard error in
Figure 6. From this figure, we observe that there is no significant
variation in the coordination number for pressures ranging from
0.1 to 10 mN/m. For higher pressures (40 mN/m or higher), a
dehydration of the polar lipid heads becomes clear. To quantify
this effect, the hydration number of the lipid head was calculated
from the radial distribution function of water around the heads
of the lipid molecules. It was found that 4.20, 4.19, 4.16, and
4.05 water molecules formed the first hydration shell around
phosphate oxygen for 0.17, 1, 10, and 40 mN/m, respectively.
In regard to the carbonyl oxygen tail, values of 1.12 (for 0.17
mN/m), 1.11 (for 1 mN/m), 1.09 (for 10 mN/m), and 0.85 (for
40 mN/m) were calculated. With these data, lipid hydration
(estimated as the sum of the hydration number of the phosphate
group) was calculated, reaching a value of 6.44 (for 0.1 mN/
m), 6.41 (for 0.17 mN/m), 6.34 (for 10 mN/m), and 5.75 (for
40 mN/m) water molecules. These values agree well with
experimental data provided by Schalke et al.9for DMPA-air-
water monolayers, where the number of water molecules that
coordinates the lipid head ranges from 6.2 to 4.8 for a surface
pressure ranging from 2 to 45 mN/m, respectively.
3.3. Hydrocarbon Tail Structure. The structure of the
hydrocarbon tail can be studied in NMR experiments by
measuring the deuterium order parameters along the lipid
ethylene tails. The order parameter tensor is defined as
where x, y, and z are the local coordinates of the system, θais
the angle made by the molecular axis with the bilayer normal,
and δab is the Kronecker delta. From simulation, the order
parameter -SCDcan be determined using the relation proposed
by Egberts and Berendsen34
where Sxxand Syyare the terms of the order parameter tensor of
eq 2.
Using eq 3, we can calculate the order parameters of a
DMPA-molecule for different surface pressures. These results
in Figure 7 can be compared with experimental data of the
DMPA-bilayer in its liquid crystalline state.20From a
comparison simulation with experimental data, we observe the
good agreement between both results at low pressures. Morover,
from the simulation we observe how the hydrocarbon structure
of the lipid tails is not perturbed at surface pressures up to 1
mN/m. Above this, increasing the surface pressures increases
the order of the lipid tails. This increase is more noticeable for
pressures above 40 mN/m, corresponding to the pressure at
which the barometric transition state of the lipids takes place
from its liquid crystalline state (LR) to gel state (L′?), which is
comparable with the liquid condensed state (LC) in lipid
Furthermore, an angle θ was defined as the angle between
the vector along the lipid chains and the perpendicular direction
to the lipid/water interface. The values of the angle θ obtained
were of θ ) 29.5, 29.7, 22.1, and 9.2° for 0.17, 1, 10, and 40
mN/m, respectively. In this regard, Schale et al.,9measured an
angle θ of 9° at 40 mN/m by X-ray, which agrees well with
our simulation results, for pressures at which bilayer and
monolayer behaviors converge. In this sense, these data confirm
the increasingly packed arrangement found in both monolayer
Figure 6. Coordination number for water molecules. Circles, carbonyl
oxygens (atom 28), and triangles, phosphate oxygen (atom 4), attending
atom numeration of Figure 2.
TABLE 2: Different Values of Coordination Number
around the Phosphorus Atoms (Atom 3) by Oxygen Atoms
(Atoms 4, 11, and 28) from Neighboring Lipids at Different
Value of Surface Pressure
surface pressure
Figure 7. Order parameters (-SCD) along hydrocarbon tails for 0.17,
1, 10, and 40 mN/m. The experimental results20are represented by
Sab)〈3 cos(θa)cos(θb) - δab〉
a, b ) x, y, z
- SCD)2Sxx
DMPA Bilayer on the Lipid/Water Interface
J. Phys. Chem. B, Vol. 111, No. 49, 2007 13729
Page 6
and bilayer as pressure increases and agrees well with the surface
area per lipid reported above.
Considering the lipid packaging, DMPA-monolayers are
arranged in hexagonal structure at high surface pressures. In
Figure 8, the center of mass of the ethylene lipid tails is plotted
in the X-Y plane (lipid plane). The hexagonal structure
associated with the gel structure L′?(in bilayers) and liquid
condensed state LC (in monolayers) can be clearly seen for a
pressure of 40 mN/m.
In addition, the lipid head orientation with respect to the
normal to the lipid plane was studied, obtaining values of 49.7,
48.8, 42.3, and 44.7° at 0.17, 1, 10, and 40 mN/m respectively.
These values show how the DMPA-heads in their liquid
crystalline state are much more oriented toward the water layer
than DPPC, where an orientation almost parallel to the surface
of the lipid layer has been reported.10,19This increase in head
orientation can be explained by the greater degree of head
hydration and lipid packaging of them than the DPPC ones. In
this regard, Schalke et al.9reported that the head orientation of
DMPA-in its liquid condensed state LC for a pressure of 40
mN/m was 47°, which agrees with our simulation result of 44.7°.
Unfortunately, no experimental data of DMPA-head orientation
in bilayers has been reported to compare with its liquid
crystalline state LR.
By means of FTIR, Lozano et al.33observed that in the
monolayers of pure DMPA-at high surface pressures the
transition dipole moment of the carbonyl group aligned with
the interface. The angular distribution function of the bonding
vector for this CdO group was calculated for each surface
pressure studied. Thus, from Figure 9 we can observe that for
high pressures, for example, 40 mN/m, the distribution takes a
sharper form with two maximum peaks at 90 and 97°, reflecting
the above-mentioned change in orientation of the carbonyl
moiety. This dense packing of a DMPA-monolayer at high
surface pressure is in a perfect accordance with experimental
3.4. Electrostatic Potential. The electrostatic potential ψ
across the lipids layers was computed from the double integral
of the charge density35
where the origin z of the electrostatic potential ψ(0) is taken at
the middle of the lipid bilayer. The electrostatic potential
computed in this way agrees with the computed charge density
using Poisson's equation without using a cutoff radius.36Hence,
Figure 10 depicts the electrostatic potential averaged on both
symmetric lipid leaflets.
Of note is the dramatic effect of surface pressure on the
potential difference at the interface, which ranges from
-0.08 V at 0.17 mN/m to -0.22 V at 40 mN/m, an effect that
is of great relevance in the electrochemistry for developing
modified electrodes using Langmuir-Blodgett's method.37
3.5. Solution Properties. Having established the lipid
structure associated to barometric transitions, the way in which
the solution properties are perturbed by the barometric isomor-
phic lipid transition was studied. For that purpose, the computa-
tion box was sliced to 19 slabs parallel to the X-Y plane (lipid
leaflet), so that slab number 10 matched the midle of lipid
3.5.1. Water and Sodium (Na+) Translational Diffusion
Coefficient, Dt. First, the diffusion coefficients of water and Na+
ions were calculated using the treatment described by Cascales
et al.,16which permitted us to estimate their diffusion coefficient
in each zone of the computing box. The results for water are
shown in Figure 11. As we can see, the regions on both sides
of the box are composed of bulk water, reaching a steady value
for the Dt,xy. Note that the diffusion coefficient of bulk water
for SPC at 349 K is 7.5 × 10-5cm2s-1,38which is very close
to our value of 6.5 × 10-5cm2s-1. Also of interest is that
there is no significative variation in the water diffusion
coefficient with respect to pressure, indicating that our conclu-
sions are not affected by any simulation artifact. From the results
depicted in Figure 11, it can be observed that there is almost
no correlation between the diffusion coefficient and surface
pressure at the lipid/water interface, where a minimum value
of around 2.5 × 10-5cm2s-1was measured in the four cases
in the vicinity of the lipid interface regardless of the isomorphic
state of the lipids.
A similar trend was observed for Na+ions, where the values
of 4.5, 4.3, 4.2, and 4.4 × 10-5cm2s-1were measured for the
bulk water and 1.2, 1.1, 1.2, and 1.2 × 10-5cm2s-1in the
vicinity of the lipid surface, respectively. These results are in
good agreement with experimental data for sodium ion in bulk
water39and in the vicinity DPPS-layers,40where values of 3.8
× 10-5cm2s-1have been reported for bulk water at 298 K
and a value of 2.1 (1.1 × 10-5cm2s-1in the vicinity of a
DPPS-bilayer at 350 K.
The first conclusion from the values reported above is that
the barometric phase transition of lipids does not perturb the
profile of sodium diffusion from the bulk water to the vicinity
of the lipid interface.
Figure 8. Lipid packing for one of the two DMPA-leaflets. Center
of mass of each lipid tail is represented by a circle. Left: surface
pressure of 0.17 mN/m. Right: surface pressure of 40 mN/m. Lines
mark the hexagonal packing of this arrangement.
ψ(z) - ψ(0) ) -1
Figure 9. Angular distribution function for the vector bonding of the
CdO moiety (atoms 27 and 28, considering the atom numeration of
Figure 2) with respect to the perpendicular axis to the lipid/water
interface and different surface pressures.
13730 J. Phys. Chem. B, Vol. 111, No. 49, 2007
Casares et al.
Page 7
3.5.2. Water Rotational Relaxation Time, τ. Using the same
regions as described above, we estimated the rotational diffusion
coefficient of water molecules.16The reorientation of water
molecules is described by the correlation function
from which an apparent mean relaxation time, τapp, can be
Within this procedure, we calculated τappfrom the beginning
of the lipid/water interface to bulk water at each surface pressure
(see Table 3). As an example, the fit of the calculated data to
eq 6 for 0.17 and 40 mN/m in the central region and near the
lipid/water interface is depicted in Figure 12.
The assumption of bulk water in the midle of the aqueous
region and the absence of any simulation artifact due to surface
pressure is again confirmed by this study. The 2.11 ps of our
simulated value is in good agreement with the 1.7 ps38reported
for bulk water at 349 K. The slight difference between the results
Figure 10. Electrostatic potential across the z-axis for each surface pressure. For clarity, each region is deliniated with dotted lines. The zero volts
is placed in the middle of the water layer.
Figure 11. Translational diffusion coefficient Dt,xyof water for different
positions in the computing box along the axis perpendicular to the lipid/
water interface and different surface pressures.
〈P1(t)〉 )∑
aiexp(- t/τi) (5)
Figure 12. Reorientational correlation function of water dipole at 0.17
mN/m (top) and 40 mN/m (down) surface pressures. Symbols:
simulation results. Line: fitted curve.
TABLE 3: Reorientational Relaxation Time (τapp) of Water
for Different Regions of the Computing Box and Surface
π (mN/m)
slab number
1 1040
DMPA Bilayer on the Lipid/Water Interface
J. Phys. Chem. B, Vol. 111, No. 49, 2007 13731
Page 8
can be attributed to the fact that in our simulations some water
molecules that were hydrating sodium ions were considered
rather than the pure water in Postma's work.38In regard to the
rotational relaxation time in the vicinity of the lipid surface, it
increased up to 11.3 (1.1 ps independently of the surface
pressure. In other words, we can assert that lipid barometric
phase transition did not perturb the dynamic properties of water
from bulk to water/lipid interface in the range of pressures
3.5.3. Sodium Ion Hydration. Using the radial distribution
function of eq 1, we are able to calculate the hydration number
of sodium ions in solution. As it is shown in Figure 13, a value
of 5.0 water molecules was estimated in bulk water and 1.0
water molecule near the lipid/water interface. The results for
bulk water agree well with experimental41and simulation42data
in aqueous solution, where values of 4.6 and 3.8 have been
reported at 298 K. At the lipid/water interface, Cascales et al.
reported40a value of 1.2 in the vicinity of a DPPS-bilayer,
which agrees with the value of 1.1 obtained here for all the
simulated surface pressures.
4. Conclusions
Four DMPA-bilayers were subjected to different lateral
pressures in a range from 0.17 to 40 mN/m. The results
demonstrated that the lipid bilayer and monolayer follow the
same behavior only when surface pressure is sufficiently high.
On the other hand, their behavior differed with low pressures.
Validation of the DMPA-force field was made by compari-
son with experimental data for both bilayer and monolayer. The
experimental data corresponding to hydrocarbon order param-
eters and surface area per lipid molecule showed excellent
agreement with our simulation results at low surface pressures,
which reproduced the DMPA-bilayer behavior. At high surface
pressures, the area per lipid molecule, orientation of the carbonyl
moiety, tail orientation, head orientation, and hexagonal ar-
rangement of the lipid tails reflected monolayer behavior in the
liquid condensed (LC) phase.
In addition, we focused our interest on how this barometric
polymorphic phase transition affects the lipid/solution interface.
Thus, the water reorientational time, the water and sodium
translational diffusion coefficient, and sodium hydration were
studied. From the simulation results, we can assert that
barometric polymorphic lipid transition does not perturb the
lipid/water interface properties in the range of the pressures
Acknowledgment. J.J.L.C. wishes to thank the Spanish
Government (Ministerio de Educacion y Ciencia, MEC) and
Fundacion Seneca for his financial support through projects
BQU-2001-04777 and 00483/PI/04, respectively. L.C., M.T.M.R.,
and J.J.G.C. thank the Spanish CICYT for financial support of
this research in the framework of Project Nos. CTQ2004-03246/
BQU and MAT2004-03849. Also, J.J.G.C. thanks the Ministerio
de Educacion y Ciencia for a Formacion Profesorado Univer-
sitario (FPU) predoctoral fellowship.
References and Notes
(1) Cullis, P.; Hope, M.; de Kruijff, B.; Verkleij, A.; Tilcock, C. In
Phospholipids and cellular regulation; Kuo, J., Ed.; CRC Press: Boca
Raton, FL, 1985; pp 1-60.
(2) Yeagle, P. FASEB J. 1989, 3 (4), 1833-1842.
(3) Nagle, J.; Tristram-Nagle, S. Biochim. Biophys. Acta 2000, 1469
(3), 159-195.
(4) Yeagle, P. L. In The structure of biological membranes; Yeagle,
P., Ed.; CRC Press: Boca Raton, FL, 1992; pp 73-156.
(5) Ichimori, H.; Hata, T.; Matsuki, H.; Kaneshina, S. Biochim. Biophys.
Acta 1998, 1414 (1-2), 165-174.
(6) R. Winter. Curr. Opin. Colloid Interface Sci. 2001, 6 (3), 303-
(7) Mo ¨hwald, H. In Handbook of biological physics; Lipowsky, R.,
Sackmann, E., Eds.; Elsevier Science B. V.: New York, 1995; pp 161-
(8) Hunt, R.; Mitchell, M.; Dluhy, R. J. Mol. Struct. 1989, (214), 93-
(9) Schalke, M.; Kru ¨ger, P.; Weygand, M.; Lo ¨sche, M. Biochim.
Biophys. Acta 2000, 1464, 113-126.
(10) Dluhy, R. Appl. Spectrosc. ReV. 2000, 35 (4), 315-351.
(11) Schalke, M.; Lo ¨sche, M. AdV. Colloid Interface Sci. 2000, 88 (1-
2), 243-274.
(12) Mendelsohn, R.; Flach, C. Curr. Top. Membr. 2002, (52), 57-88.
(13) Pedrosa, J.; Perez, M.; Prieto, I.; Martin-Romero, M.; Mo ¨bius, D.;
Camacho, L. Phys. Chem. Chem. Phys. 2002, 4 (11), 2329-2336.
(14) van Gunsteren, W. F.; Berendsen, H. J. C. Angew. Chem., Int. Ed.
Engl. 1990, 29 (9), 992-1023.
(15) Alper, H. E.; Bassolino-Klimas, D.; Stouch, T. R. J. Chem. Phys.
1993, 99 (7), 5554-5559.
(16) Cascales, J.; Berendsen, H.; Garcia de la Torre, J. J. Phys. Chem.
1996, 100 (21), 8621-8627.
(17) Tieleman, D.; Marrink, S.; Berendsen, H. Biochim. Biophys. Acta
1997, 1331 (3), 235-270.
(18) Kaznessis, Y.; Kim, S.; Larson, R. Biophys. J. 2002, 82 (4), 1731-
(19) Cascales, J. L.; Otero, T.; Romero, A. F.; Camacho, L. Langmuir
2006, 22 (13), 5818-5824.
(20) Pott, T.; Maillet, J.; Dufourc, E. Biophys. J. 1995, 69 (5), 1897-
(21) Wu, F.; Gericke, A.; Flach, C.; Mealy, T.; Seaton, B.; Mendelsohn,
R. Biophys. J. 1998, 74, 3273-3281.
(22) Mendelson, R.; Flach, C. Curr. Top. Membr. 2002, 52, 57-78.
(23) Berendsen, H.; Grigera, J.; Straatsma, T. J. Phys. Chem. 1987, 91
(24), 6269-6271.
(24) Lindahl, E.; Hess, B.; van der Spoel, D. J. Mol. Model. 2001, 7
(8), 306-317.
(25) Berendsen, H.; van der Spoel, D.; van Drunen, R. Comp. Phys.
Comm. 1995, 91 (1-3), 43-56.
(26) Hyperchem 7.5. HyperCube, Inc.
(27) Darden, T.; York, D.; Pedersen, L. J. Chem. Phys. 1993, 98 (12),
(28) Essmann, U.; Perea, L.; Berkowitz, M.; Darden, T.; Lee, H.;
Pedersen, L. J. Chem. Phys. 1995, 103 (19), 8577-8593.
(29) van Gunsteren, W.; Berendsen, H. Mol. Phys. 1977, 34 (5), 1311-
(30) Berendsen, H. J. C.; Postma, J. P. M.; van Gunsteren, W. F.; DiNola,
A.; Haak, J. R. J. Chem. Phys. 1984, 8 (8), 3684-3690.
(31) Blume, A.; Eibl, H. Biochim. Biophys. Acta 1979, 558(1), 476-
(32) Ziegler, W.; Blume, A. Spectrochim. Acta, Part A 1995, 51, 1763-
(33) Lozano, P.; Fernandez, A.; Ruiz, J.; Camacho, L.; Martin, M.;
Mun ˜oz, E. J. Phys. Chem. B 2002, 106 (25), 6507-6514.
Figure 13.
surrounding the Na+ions for different surface pressures and regions
of the computing box.
Values of coordination number for water molecules
13732 J. Phys. Chem. B, Vol. 111, No. 49, 2007
Casares et al.
Page 9
(34) Egberts, E.; Berendsen, H. J. Chem. Phys. 1988, 89 (6), 3718-
(35) van Buuren, A.; Berendsen, H. Langmuir 1994, 10 (6), 1703-1713.
(36) Pandit, S.; Bostick, D.; Berkowitz, M. Biophys. J. 2003, 84 (6),
(37) Goldenberg, L. J. Electroanal. Chem. 1994, 379 (1-2), 3-19.
(38) Postma, J. A molecular dynamics study of water. Ph.D. Thesis,
Rijkuniverseit Groningen, The Netherlands, 1985.
(39) Lide, D., Ed. Handbook of Chemistry and Physics; CRC Press:
Boca Raton, FL, 2002.
(40) Cascales, J. L.; de la Torre, J. G. Biochim. Biophys. Acta 1997,
1330 (2), 145-156.
(41) Bockris, J.; Reddy, A. Modern Electrochemistry 1. Ionics; Plenum
Press: New York, 1998.
(42) Impey, R.; Madden, P.; McDonald, I. J. Phys. Chem. 1983, 87 (25),
DMPA Bilayer on the Lipid/Water Interface
J. Phys. Chem. B, Vol. 111, No. 49, 2007 13733


text:
Happy Valentine's Day from HRC
February 14, 2013 by HRC staff
Post submitted by Eric Cameron, HRC Digital Media Specialist
Here at HRC our work is guided by the idea that, regardless of gender, love and commitment are worthy of celebration and equal recognition under the law.
So whether you anticipate it all year long or it's just another day on the calendar, join us this Valentine's Day in celebrating the love that unites us and drives us forward in the fight for LGBT equality.
Read some of the inspiring stories of love, marriage and commitment that have already been submitted to our Share Your Love photo project, and don't forget to submit your own today!
Top Posts
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February 14, 2015
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February 16, 2015
"Love Has No Labels" Ad Campaign Celebrates Diversity and Inclusion
February 18, 2015


text:
Bar Nunn - Page 2
Police Standoff In Bar Nunn
Bar Nunn Man Pleads Guilty to Conspiracy to Commit Larceny Charge
A Bar Nunn man who prosecutors say conspired to steal $15,000 in generator wire changed his initial plea in Natrona County Court on Friday.
Danney Pollock faces up to 10 years in prison after pleading guilty to one count of conspiracy to commit felony larceny in a plea deal that would drop another conspiracy charge and a pair of larceny charges
Public Meeting Ponders New I-25 On/Off Ramp For Bar Nunn
A conversation about a possible new interchange for I-25 north of Casper happens next Tuesday. Wyoming Department of Transportation Spokesman, Jeff Goetz says its very early in the considerations and they want to hear from local residents first. It'll happen at Bar Nunn Elementary and he says they're mo


text:
Here is a link to our fishing items for kids!!! We will be adding more as they come in.http://www.copesdistributing.net/kids-fishing-gear-c-242_248.html
[SIGPIC][/SIGPIC] You can check us out online at www.copesdistributing.netYou can call us 1-866-523-2673 ask for Carmen Misty or Geri.WE CHANGED OUR HOURS:Monday-Friday 9:00 A.M. - 5:30 P.M. NEW SHOP NUMBERS ARE:937-459-5208937-459-5247937-459-5318937-459-5361To talk to me press EXT 201You can be our friend on Facebook:http://www.facebook.co...me.php?
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Looks like a great start. Dads are gonna have a hell of a time telling their daughter no when it comes to that barbie setup.
I don't need No stinking signature.
I hate to admit this but my wife has a pink pole that lights up in rainbow colors when you work the reel.
My buddy broke his pole before a trip and I told him I had him covered.
I about pissed my pants when I handed him the pole. He was really angry when he started to reel in a fish and it lit up.
I almost fell off the dock I was laughing so hard.
Ty not as nice as going fishing with grand kids though.
I sent a link to the site to him. I think he is overdue for getting his 4 kids (3 girls 1 boy) fishing. His girls will love the barbie setup and little bubba is all about cars.


text:
8100 N. TroostKansas City, MO 64118Phone: (816) 413-6800Attendance: (816) 413-6801Nurse: (816) 413-6802Fax: (816) 413-6805
NKC Schools Community Education Services presents Children & Youth Classes to get the ball rolling in 2015. Click here!
Please join PTA!
You can find more PTA info at:
Please support our Clardy Scholar Program by attending our Chuck E. Cheese Fundraising Night on April 3rd.
All students who attend will receive 10 free tokens!
The class with the best attendance will receive a Pizza Party with Chuck E!
Nationally & State Accredited


text:
Winners of this year's Video Ezy Entertainment Awards were announced at an inaugural star-studded ceremony held at the NIDA Parade Theatre this evening.
The event kicked off with a red carpet cocktail party attended by the who's who of the Home Entertainment Industry including presenters Bill "Mr Movies" Collins, Rebekah Elmaloglou, 'At the Movies' hosts Margaret Pomeranz and David Stratton, Fenella Kernebone and Marc Fennell of "The Movie Show", and FHM model Rozz Switzer.
The Awards, aimed at recognising movies that people loved watching at home in 2004, also raised $20,000 for the Starlight Children's Foundation. The much needed funds will go towards making life brighter for seriously ill children around Australia.
"Since the inception of the Video Ezy Entertainment Awards, we have had hundreds of thousands of public votes, clearly demonstrating how much Aussies love their movies, and love coming to their local Video Ezy store for the variety, fun and value we provide."
"We are extremely pleased with tonight's result and are proud to be donating to the Starlight Children's Foundation, whom we have had a longstanding relationship with," said Steve Johnston, CEO, Video Ezy.
Louise Baxter, National Strategy & Business Manager, Starlight Children's Foundation said:
"The funds we received from Video Ezy this evening will go towards granting the wishes of some of the many seriously ill children who are waiting to have a little magic in their lives. We are very grateful to Video Ezy, and look forward to a continued association in future."
Hosted by comedians Scared Weird Little Guys, the night was kept upbeat with presenters asked to bestow winners of each of the 10 categories with an award. Lord of the Rings: The Return of the King dominated the night, winning five of the 10 categories including Best Picture 2004.
Category winners were:
Best DVD Release 2004
The Lord Of The Rings: The Return Of The King (4-Disc Edition)
Roadshow Entertainment
Best Picture 2004
The Lord Of The Rings: The Return Of The King
Roadshow Entertainment
Best Duo 2004
Frodo And Sam (Elijah Wood & Sean Astin) – The Lord Of The Rings: The Return Of The King
Roadshow Entertainment
Best Hero 2004
Captain Jack Sparrow (Johnny Depp) – The Pirates Of The Caribbean: The Curse Of The Black Pearl
Buena Vista Home Entertainment
Best Heroine 2004
The Bride (Uma Thurman) Kill Bill Volume 1
Buena Vista Home Entertainment
Best Villain 2004
Gollum (Andy Serkis) – The Lord Of The Rings: The Return Of The King
Roadshow Entertainment
Best Breakthrough Performance 2004
Puss In Boots (Antonio Banderas) – Shrek 2
Universal Pictures Video
Best Performance By An Animal 2004
Dory (Ellen DeGeneres) Finding Nemo
Buena Vista Home Entertainment
Best Action Sequence 2004
The Battle For Minas Tirith- The Lord Of The Rings: The Return Of The King
Roadshow Entertainment
Best Chick Flick 2004
50 First Dates
Sony Pictures Home Entertainment
Following the Award ceremony, attendees were treated to an unforgettable after party, with a surprise guest performance by songstress Christine Anu, accompanied by piano men Howl at the Moon.
Thanks to 'Elizabeth'
- The Lawnmower Man cuts From Dusk Till Dawn - April 1, 2015
- Trailer : Mad Max : Fury Road - April 1, 2015
- It Follows expanding any further this Friday! - April 1, 2015
- Trailer : Masterminds - March 31, 2015
- When will we see the next Star Wars trailer!? - March 31, 2015
- Shrek to appear in new Puss in Boots sequel? - March 31, 2015
- Disney developing live-action Mulan - March 31, 2015
- Tron 3 ascends into title territory - March 31, 2015
- Superhero Movie Updates : X-Men, Suicide Squad, Deadpool, The Flash, TMNT - March 31, 2015
- NBC bringing The Wiz to Television! - March 31, 2015
Against the Current - the band, not adventures in dangerous swimming 101
Zedd - If our love is tragedy, why are you my remedy? (Well, answer my question!)
Arrow (Okay, Felicity from Arrow!)
Chrissy Costanza (cat eyes and buttery lyrics!)
Girls (TV) (Okay, Allison Williams!)
Movies - especially when they play in the dark.
Twin Peaks (TV)
Friends (TV) (It had me at "No way are you cool enough to pull Clint"; damn straight, Chandler!)
Traveling - preferably where water is, so I can splash someone!
Star Wars trilogy - no, the other one, fella!
Alex G - far more talented than her younger brother Alex H
Cameron Crowe movies - Say Anything..., Jerry Maguire, Almost Famous
The sign 'Free Wi-Fi'.
Reenacting dance/song scenes from "Grease" with my little girl (hey! Wait till you see my 'Summer Lovin'! - don't judge)
Die Hard - 40 stories of Sheer Adventure!
Alex Goot & Friends (his enemies aren't half as talented!)
Cooking up a nice dish and sitting in the entertainment area, on a cool night, basking in it's greatness.
Inflatable kids pools full of Vodka Lime Crush.
Acidic Email from angry, over passionate teenagers after I trash something "Twilight"-related on the site. Sparkle elsewhere.
My baby girl's big, caring heart.


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Dragons Den Peter Casey to run in Donegal as Independent
- Politics
- March 25, 2015, 07:52
Dragons Den entrepreneur Peter Casey has confirmed he will contest the upcoming General Election in Donegal as an Independent candidate.Mr Casey, from(...)
Our wedding story: 'He knew before our first date that he'd marry me'
- People
- March 21, 2015, 06:00
Each Saturday on irishtimes.com/weddings we'll report on couples' love stories. We'll tell the tales and show the pictures of these fabul(...)
US veteran pleads not guilty to Islamic State terror charges
- March 19, 2015, 07:46
A US air force veteran has appeared in court accused of trying to support the Islamic State.Tairod Nathan Webster Pugh (47) from New Jersey pleaded (...)
Jersey Boys writer: 'Frankie Valli is a dark soul. He is not a happy guy'
- People
- March 13, 2015, 06:00
Rick Elice seems anxious to please his Irish audience. As he delivers a self-deprecating and smart monologue about how he came to write Jersey Boys(...)
Delta plane skids off runway at snowy New York airport
- March 5, 2015, 20:38
A Delta Air Lines jetliner landing during a snowstorm at New York's LaGuardia Airport on Thursday slid off the runway and struck a fence before coming(...)
Sinn Féin's evolving funding stream from Irish-America
- Politics
- March 5, 2015, 03:30
The drink flowed as diners chewed on filet mignon and enjoyed a rousing political speech in between traditional Irish songs performed by a band in on(...)
Where Friends of Sinn Féin raises money in the US
- Politics
- March 5, 2015, 03:30
An examination of the 20 years of financial information in the group's US filings shows the success the party has enjoyed in tapping the Irish-America(...)
The New York street baby's first birthday
- Generation Emigration
- February 20, 2015, 13:15
The place of birth on Ila Isabelle McCourt's birth certificate reads "Manhattan". Nothing unusual there. Ila is a native New Yorker, born to Cian M(...)
Scientist concerned over 'CIA' weather weapon query
- Environment
- February 15, 2015, 17:02
A senior US scientist has expressed concern that the intelligence services are funding climate change research to learn if new technologies could be u(...)
Republican hopefuls heading to UK to boost credentials
- February 2, 2015, 01:00
New Jersey governor Chris Christie on Sunday began a three-day trade mission to the UK. The trip is meant to bolster his state's economic and cultura(...)


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Seeking 15" undercounter icemaker that produces cubes--impossible
Hi there,
I'm looking for an ice maker that produces actual cubes, not nuggets or crescents, and that can fit in a very small space, only 15" or so wide and not more than 24" deep. Does such an animal exist? I've had real trouble finding any that produce cubes, apart from size. Then I thought maybe we should just make our own--but how to store when we have a big party? Any help much appreciated.
I think only countertop and large commercial ice machines make cubes.
Hoshizaki makes an undercounter 15" ice machine that makes top hat type ice. It is hard clear ice and has a slow melt time to not water down drinks and does not stick together or against the side of the glass.
IMO Hoshizaki makes the most reliable and durable residential ice machines. Although a Japanese company these are made in Georgia.


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It got a ton of attention, US players got pissed because Sega wouldn't bring the game out here sooner, figured out how to use a translator third party app made by psupedia guys, then started fucking with the game, leading to japanese players hating anyone speaking english on their servers...and english speaking people damning the US. Iirc, sega started banning anyone playing outside of japan too, which was funny.
If it ever comes out in the US, I may try it, bt as of now fuck this game and fuck Sega too.


text:
Quick Links
Local Control Accountability Plan (LCAP)
Online Bidding for Warehouse Supplies
Technology Request For Proposal - RFP
Transitional Kindergarten - TK
Contact Info
Turlock Unified School District
1574 E. Canal Drive
Turlock, CA 95380
(209) 667-0645
Mailing address:
Turlock Unified School District
PO Box 819013
Turlock, CA 95381-9013
(209) 667-0645
Email Us
To search and apply for open jobs for both certificated and classified openings. Use the keyword "Turlock Unified" and our current open positions will display or click on the link below. Complete an application through the Edjoin website:
School Search
If you would like to know which school your student(s) will be attending, click on the following link and enter your home address information, your school based on grade level will appear.
Please note: This is only for neighborhood schools or schools of residence, this does not apply to lottery based schools at Osborn Two-Way Immersion, Walnut Education Center or Dutcher Middle School.
School Websites
The School websites addresses have changed:
Osborn Two-Way Immersion Academy
Sandra Tovar Medeiros Elementary
Sub Info
Festival on the Green ~ 2015
The 2015 Festival on the Green was a resounding success. Each school was represented in either choir, orchestra or band. It was a showcase of their wonderful talents and shows strong support for the music programs at Turlock Unified School District.
Thank you to the Turlock Education Foundation, the Music Educators, parents, students and support staff who made the musical day possible.
Whose Cuisine Will Reign Supreme?
Join us to see whose dish will reign supreme in this Iron Chef style competition between Turlock High and Pitman High and their respective culinary arts program. The event will also include live entertainment, a 50/50 raffle, an auction, and much more!
TurlockEdFoundation.org/ironchef - Sponsorships Available
CSU Stanislaus Offering Math Camp during Spring Break
CSU Stanislaus
Math Camp
Spring 2015
What: Interactive hands on learning experience with games and puzzles.
Where: At CSU Stanislaus DBH Building #21
Map: https://www.csustan.edu/campus-maps
When: April 6th - 10th 1:30 PM - 3:30 PM
For Who: Students in grades 2nd - 5th
Cost: $50.00 per student (T-shirt included)
How to Register: Online registration and payment at: https://commerce.cashnet.com/Math1
Contact Us
-Email: email@example.com
- Phone: (209) 667-3780
- Website: https://www.csustan.edu/math/grants-program
Calendar of Events
Spring Recess
Health Information for Parents on Enterovirus D68
Due to the recent outbreak of the enterovirus D68 which is associated with severe respiratory illness that has been especially harmful to children, we would like to provide resource information that is available to answer any questions you may have:
TUSD Family Link
The website address to access Turlock Aeries Family Link is:
The Aeries Family Link program is a valuable tool in communicating with the school staff concerning your students' academic success. For information on how to sign up for the program, please check with the school office for more information.
TUSD on Facebook and Twitter
District Office Telephone Directory


text:
There are no games scheduled for Tuesday, Nov 22.
8:00 PM ET, November 22, 2005
BATON ROUGE, La. (AP) -- Even though Seimone Augustus was held to her lowest point total in two seasons, No. 3 LSU cruised to an easy victory in its home opener.Augustus played just 20 minutes and had 10 points to help LSU rout Southern University 107-39 on Tuesday night. It was her lowest scoring game since finishing with eight points against Richmond two seasons ago.Sylvia Fowles scored 21 points and Erica White added 14 for the Lady Tigers (2-0). LSU forced 34 turnovers and held the Lady Jaguars (2-1) to 32 percent shooting, including 2-for-10 from 3-point range."Other than a sluggish start of the second half, I was really pleased," LSU coach Pokey Chatman said. "We did a good job in both our half-court offense and our half-court defense."Ashley Thomas and Quianna Chaney each scored 12 for the Lady Tigers, whose 68-point margin of victory in their home opener ranked second in team history to a 104-28 win over Prairie View 10 years ago."Their athleticism is incredible," said Southern coach Sandy Pugh. "Some of those kids on that team, they could probably play for our men's team. They're so athletic, quick, jump well, strong. Very impressive."Rolanda Monroe led Southern with 11 points. Fredreika Lewis added 10."We really never got into our offensive schemes until the second half to where we were able to get the ball down and do something positive with it," said Pugh. "I thought that was a big problem. Their athleticism was over the top for us."In the first half the Lady Jaguars shot 67 percent, but trailed 21-9. Southern made four of its first six field goal attempts, but committed nine turnovers."We limited Southern's touches in the half-court," Chatman said. "Our pressure was good early. We got all five people involved in our defense."Fowles, who scored 15 points in the first half, made the last two baskets before halftime to give LSU a 57-17 lead."Offensively as a team our execution was as good as it's been in a long, long time," Chatman said. "You could go from players one through 12 and I was pleased."
Copyright by STATS LLC and The Associated Press


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On the geometry spec-sheet Yeti lists different head angles for the 32 and 34 stanchion forks at the same length. Specifically they state that a 32 stanchion 150mm travel fork results in a 67 degree head angle while a 34 stanchion 150mm travel fork results in a 66.7 degree angle...
What gives?...
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LOGAN — Mark Weber, who spent the last six seasons as an offensive line coach at BYU, has been named Utah State's offensive line coach and assistant head coach.
Weber has 34 years of collegiate coaching experience and has been involved in 15 bowl games. During his time at BYU, Weber coached six first-team all-Mountain West offensive linemen, including two All-Americans in Dallas Reynolds and Matt Reynolds.
"Mark Weber is a veteran offensive line coach with a background as a tough, fierce competitor," said USU head coach Matt Wells in press release Monday. "Mark brings a great deal of experience with him to Utah State and has tremendous knowledge about football in the state of Utah. His time spent in the Mountain West Conference will aid us as we transition into a new league, and we welcome Mark and his family to Logan."
The feeling seems mutual.
"I'm really excited to work for Matt Wells. There is great energy and momentum with the Aggie program and I'm thrilled to get the chance to help in its growth," said Weber. "Matt is innovative and energetic and did a fantastic job as Utah State's offensive coordinator. I love the way those offensive linemen play with their mindset and attitude, and I can't wait to be part of that program and around those kids."
Prior to his stint at BYU, Weber spent one season at North Carolina (2006) and two years at Fresno State (2004-05), where he mentored All-America Logan Mankins, a first-round pick of the New England Patriots, and Cole Popevich, who was a freshman All-America.
Weber spent seven seasons at UCLA (1997-2003) prior to his stint at Fresno State, helping guide the Bruins to five bowl appearances, including the 1999 Rose Bowl and a pair of Pac-10 titles. At UCLA, he coached two first-team All-Americas, including Kris Farris, who won the 1998 Outland Trophy. He also mentored three freshmen All-Americas and six players who garnered all-Pac-10 honors.
Weber and his wife, Kathryn, have two daughters, Allie and KaCee.
Mark Weber File
Coaching History:
2013- Utah State – Assistant Head Coach/Offensive Line
2007-12 BYU – Offensive Line
2006 North Carolina – Offensive Line
2004-05 Fresno State – Offensive Line
1997-2003 UCLA – Offensive Line
1994-96 UNLV – Assistant Head Coach/Offensive Line
1993 Nevada – Assistant Head Coach/Offensive Line
1991 Missouri Western State College – Defensive Line
1987-90 Oregon State – Offensive Line
1985-86 West Texas State – Assistant Coach
1983-84 Eastern New Mexico – Assistant Coach
1982 Snow College – Assistant Coach
1980-81 Idaho State – Graduate Assistant
1979 Los Angeles Valley College – Assistant Coach
Playing Experience:
1978-80 Cal Lutheran – Offensive Guard
1980 Cal Lutheran – Physical Education
1981 Idaho State – Physical Education (masters)
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Phoenix Suns
Robert Sarver is NOT looking to sell the Suns
Late Monday night, NBA reporter Ric Bucher published a note regarding the Phoenix Suns.
Discussing the team's search for a new general manager, Bucher also said "The bigger question is, how much longer will Sarver be calling the shots? One source says the increased evaluation of franchises inspired by the Seattle-Sacramento fight over the Kings has Sarver wondering if it's time to cash out."
That, naturally, piqued the interest of Suns fans everywhere, many of whom are not pleased with how Sarver has run the team since taking over in the summer of 2004.
But not so fast, says Arizona Sports' John Gambadoro.
Contrary to a report on Robert Sarver wondering if its time to "cash out" there is zero truth to it. The Phoenix Suns are not for sale.— John Gambadoro (@Gambo620) April 30, 2013
Well there you go.
Sarver and the Suns have a difficult road ahead of them.
The team finished the 2012-13 seasons with a 25-57 record, which was the worst in the NBA's Western Conference and ranks as the second-worst mark in franchise history.
President of basketball operations Lon Babby and the organization chose to part with general manager Lance Blanks, and the search for his replacement is currently underway.
Once hired, the new GM will be tasked with choosing wisely with the multitude of draft picks the Suns have coming to them over the next few seasons, as well as rebuilding a roster that lacks consistency and star power.
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- Tim Kempton, Suns analyst and Notre Dame alumnusTK explains what Notre Dame has to do to have a chance against Kentucky in the Elite Eight.
- Mike D'Antoni- Former NBA head coachYou won't see Mike D'Antoni coaching college basketball.
- Lon Babby- Suns president of basketball operationsLon Babby says the Suns gave up an opportunity in their playoff race.


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written before about the excellent fantasy series A Song of Ice and Fire by George R.R. Martin. As I've mentioned, one of my most anticipated television series is HBO's adaptation of the first novel, A Game of Thrones.
The first teaser, embedded below, is short but enticing, nicely capturing the foreboding tone of the beginning of the epic saga.
The cast seems perfectly selected: Mark Addy as King Robert Baratheon, Sean Bean as the heroic Eddard Stark, Peter Dinklage as my favorite character Tyrion Lannister, Lena Headey as the beautiful but scheming Queen Cersei Lannister, Emilia Clarke as the dragon heiress Daenerys Targaryen, and many other great actors.
The epic tale deals with multiple families battling for control of the land of Westeros, on a world where seasons last decades, supernatural creatures exist, and sorcery is real. The fantasy elements are almost a sidenote to the realistic drama and rich characters that Martin has created, with loads of sex and violence, all of which will be brought to life in HBO's eagerly awaited version.
Tune in to Game of Thrones in 2011!
News: New Girl, Americans, House of Lies, Bloodline renewed, Sky1 and Stan Lee team up + more - I offer no guarantees any of these are true… *Film casting* - Adewale Akinnuoye-Agbaje to play Killer Croc in *Suicide Squad* - Stephen Amell to p...
26 minutes ago


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Order Bessarabian Stamps today!
"Terribly good!" —Andrei Codrescu
Reminiscent of Bruno Schulz's Street of Crocodiles, Oleg Woolf's Bessarabian Stamps—a cycle of sixteen stories set mostly in the Moldovan village of Sanduleni—is a vivid, surreal evocation of a liminal world. Sanduleni's denizens are in permanent flux, forever shifting languages, cultures, and states, in every sense of the word. With a warm, Bessarabian irony recalling one of Eastern Europe's long-forgotten regions, the Stamps explore what it means to live on the edges of empires, which rise and fall while Sanduleni abides.
"I don't know if Bessarabian Stamps is footnote, photography, short story, or what. I vote for poetry. Terrific poetry!" —Gerald Stern
Click here to order your copy of Oleg Woolf's Bessarabian Stamps, translated by Boris Dralyuk, directly from Phoneme Media.


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Boys Basketball: Spread out scoring keys DL's Mid-State win over Cards
The visiting Staples-Motley boys' basketball team didn't know where the next Detroit Lakes' points were going to come from Thursday inside Ralph Anderson Gym, as a dozen different Lakers scored in their convincing 64-44 victory.
Are you a newspaper subscriber but you don't have a Digital Access account yet? https://secure.forumcomm.com/?publisher_ID=40&event=subscriber/lookup.
You will need your subscription account number and phone number. Not sure if you have an account? Email us at firstname.lastname@example.com and we can help you.


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Parent to Parent
A place for parents to talk about life's most challenging job
By Amy Graff
Posts filtered on Category
Sometimes we parents need a laugh
Remember to post your funny kids story here. For inspiration, Jenn told an adorable story today about Andrew, who pulled his little potty seat into the middle of the living room. When Jenn asked if she could help, he told her "I need privacy." [Read More]
Overheard on Facebook: What does 'sexy' mean?
A mom says: My 8 year old son asked me what "sexy" meant tonight? I wanted to say "I'll get back to you on that." My reply: "Sex is another word for gender. So there are two sexes: male and female. So when someone says 'sexy', they mean that a female or male is very […] [Read More]
Kids say: Fitness and exercise
Make sure to check out and see if your comments on chores several months ago made our first Parent to Parent page in Unwind this Sunday. In the meantime, we're gathering content for the following Sunday's theme — fitness and exercise (for parents). And we're looking for funny stories about things your kids have said […] [Read More]
Share your funny stories about kids and chores
A few months ago, we wrote a post asking for tips for getting your kids to help out around the house, and you had great ideas. Now, we're looking for funny stories related to chores — either excuses kids make to get out of them or helping-out attempts gone wrong. Andrew has attempted to feed […] [Read More]
Elmo does his version of LMFAO's 'Sexy and I Know It'
My favorite line from "I'm Elmo and I Know It" is: "We head to the store so we don't be nervous/No shoes, no shirt, and I still get service/Wha. [Read More]
Freestyle baby swinging — or an Internet hoax?
I was told to check out this crazy video of "freestyle baby swinging" when I got to the office today. Apparently this video has gone viral. There's no way this can be real or this woman would be behind bars by now. I think it's a joke, but it's not very funny, is it? Freestyle […] [Read More]
Parents: Take charge in 2012
Have you made your New Year's resolutions already? We have some ideas to get your parenting under control in 2012 — along with your own goals, of course, to lose your love handles and stop saying "yes" to every PTO position. [Read More]
Send us your kid bedhead photos
I have a fun idea for a gallery. I don't know about your kids, but Matilda gets the best bedhead in the world. I want to see if anyone else can rival her, so if your kid has particularly wild morning hair, snap a photo and send them my way (firstname.lastname@example.com). I'll put them together […] [Read More]
Our visit to Santa. Maybe next year.
Update: See Jen Smith's Christmas photo outtakes here. We visited Santa on Black Friday, as we always do when my parents come for Thanksgiving. This year, I had high hopes. Both kids have been rambling on about Santa Claus, and Andrew has been talking about how Santa is going to bring him his one Christmas […] [Read More]
Show More


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Columbus Double Murder Suspect Wounded By Police
Columbus police say a double murder suspect was shot and wounded Wednesday by officers near Parsons Avenue and Kossuth Street. He was taken into custody shortly after the killings.
Listen to the Story
A northeast Ohio injection well operator run by a man accused of illegally dumping fracking wastewater into a storm sewer is asking a state panel to overturn an order revoking its operating permit.
Both sides agree on one thing – the act that Ben Lupo is accused of is a bad deed. Michael Cyphert is the attorney for Lupo's company, D&L Energy of Mahoning County.
Ben Lupo's a bad person. He intentionally directed someone to put brine down a sewer. He shouldn't have done that. He shouldn't have done that. But are those actions to be imputed to D&L Energy simply because he's an officer of the company or was an officer at the time?
Cyphert claims that Lupo runs several companies, including Hardrock Excavating, which does brine transport, storage and disposal, and D&L Energy, which develops and drills injection wells. And Cyphert says isn't in the brine business. Cyphert says Lupo ordered an employee of Hardrock Excavating, not D&L Energy, to dump the brine – an act he describes as "reprehensibleâ€.
"We don't know why he did it. Perhaps we will not know until his trial on the federal level and he is able to put forth any defenses that he has and a jury of his peers may decide whether he's actually guilty or not.â€
Cyphert says D&L didn't benefit from the act but Hardrock might have, since it would have saved that company money on brine storage. And he also argued that the Ohio Department of Natural Resources' oil and gas division chief Rick Simmers didn't have the authority to revoke D&L's injection well operating permit over what he called "a silly, stupid, intentional act†done by an officer of another company.
"If the chief wishes to issue an order against those who are responsible, it's certainly within his prerogative to do so. But don't punish D&L Energy for something it didn't do.â€
Brian Ball is the attorney for the state, and said D&L Energy entered into contracts with companies that were not registered and not maintaining compliance with state law – and therefore the company violated the terms of its own permit. And he says there were three companies working together on this operation – and Lupo was in charge of all of them.
I know the witnesses that the state will present will show that in many times they knew they were talking to Ben because he was the heart and soul and the commonality between the consortium of companies located at this address – companies that were often at least in the three considered – Mohawk, Hardrock and D&L Energy – conspiring and causing unlawful activity with systematic routine practice.
And Ball says it's clear why Lupo made the choice he did – Ball says Lupo's company was paid to handle fracking wastewater, and so he ordered the dumping of that fluid into the storm sewer to make money.
"Some of it wasn't going to go in the ground. Some of it was going to go on the ground. And routinely at night with his direction. And that is strictly the business they were in.â€
Bottom line, says Ball, is that the chief of the oil and gas division is allowed to issue orders to enforce the terms and conditions of permits. And Ball says as the head of all three companies is responsible for the actions of any one of them.
"While Mr. Lupo's acts present personal liability for him. Liability for violations of Chapter 1509 is strict, joint and several. Therefore, as persons, D&L Energy, Hardrock and Mohawk are also all liable.â€
Testimony continues in the hearing – a decision on D&L Energy's continued operations could happen this week.


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Janet Yellen says "secular stagnation" is a potential concern for the Fed. Here's what that means, and why it matters to policy.» Read More
Expect gold to bounce when it retests April's low for the first time. But after that, there could be more trouble.
Gold opened up much lower on Sunday night, as metals took a beating once again. Silver was down as much as 8 percent, dipping to $20.25. Gold tested our next major support level, putting in a low at $1,336.30.
(Read More: Gold Heads for Longest Slide in Four Years)
Since the open, things have stabilized. Gold has reached back above $1,350, testing the electronic close from Friday, although still down more than $10 from the marked floor close. Silver has erased much of its losses, but is still down sharply on the day.
Much of this choppiness can be attributed to currencies, as the dollar index hit a high of 84.40. As we have said many times before, a stronger dollar will put pressure on commodities priced in dollars. Aside from currencies, though, gold's weakness has been no secret. Especially when the Consumer Confidence reading came in a six-year high late on Friday, there was additional selling into the close.
Look for the prior $1,335.60 low to act as a major support now, but note a retest will likely send this market lower, with less emphasis on the $1,321.50 April low. The next major downside level is $1,283.50, but as we have previously mentioned, a close below that could potentially send this market down to $1,154.
Gasoline has risen 1.5 percent in the past week, even while crude oil has dropped. This even though the two products usually move together.
Gold bears need a close below $1,368, and bulls needs a close above $1,404.
On Thursday night, gold retested the Thursday session low of $1,368, which was put in early in the day. Friday's lower price action follows a rally off of the lowest level seen in four weeks, which allowed gold to get a marked close of $1,386.90 on Thursday.
On Friday morning, the low of $1,371 has provided some support, as gold tests the major $1,383.90 resistance and retracement level.
Thursday's recovery found major resistance against Wednesday's support shelf at $1,389. It will be important for the bear camp to see a close below $1,383.90, but psychologically, it will be more important to see sustained price action below $1,380.
The trend is clearly bearish, and action in the low $1,370s will likely break the camel's back and push gold to new lows. The next major support is comes in at $1,335.60, which was the low of a reversal day. Below that, gold is supported at the previous low of $1,321.50.
Price action above $1,383.90 will help the bulls gain some intermediate-term control. Although resistance will be found at $1,389 as well, sustained price action in the mid-to-high $1,380s will likely find buyers into the end of the week, bringing the market to the next resistance level at $1,396.20 to $1,397.
Given those levels, here are two short-term out-of-the-money options plays on the gold futures that you may want to consider. Bullish traders should consider buying the June 1,420-strike calls for $900, which provides 11 days of long gold exposure. Bearish traders should consider buying the June 1,335-strike puts for $1,000, which will provide them with 11 days of short exposure.
Whatever your bias in gold, futures options are liquid and trade nearly 23 hours a day.The idea here is that in a volatile market, options can be used to trade with calculated risk.
Good luck and good trading.
Peter Schiff of Euro Pacific Capital has been bullish on gold for a long time, but now he has a new reason to buy: Japan.
That nation's sweeping plan to boost its economy has seen the Bank of Japan pledge to buy 7.5 trillion worth of government bonds a month (about 70 percent of new Japanese issuance), and Japan is embarking on stimulus in the form of government spending.
Japan's first-quarter gross domestic product data showed that its economy grew by 0.9 percent on a quarterly basis (3.5 percent annualized), which has led to a degree of optimism that Prime Minister Shinzo Abe's policies are working.
But Schiff was steadfast in his belief that Abe's plans will come to grief and that the prime minister has an unsustainable situation on his hands in aiming for a 2 percent inflation rate.
(Read More: Japan's Economy Boosted by Consumption, Exports)
In an April blog post, Schiff wrote that "the idea that informs Abe's plan, that rising prices entice consumers to buy before prices go up, is clearly suspect as economic law dictates that demand increases when prices fall."
Thus, he said, the plan is destined to fail—and quickly.
"Japan will unleash an inflation tsunami if it tries to keep its bond bubble from popping with more qualitative easing," Schiff said. "If it shows restraint, the bubble will burst, eventually taking stocks down with it."
In other words, Japan is doomed to inject increasingly more money into the system, spurring inflation, which will in turn dramatically boost the price of gold, according to Schiff.
"Gold is a buy, and the Federal Reserve and Bank of Japan policies will drive the metal to new highs," he said. "So will the easy-money policies of other central banks that are joining the currency war."
Given that gold's record price is more than $1,900 and the metal now sits below $1,400, Schiff has good reason to pick up a few ounces.
Wednesday was a tough day for gold as it dropped below $1,400 for first time in nearly a month. But traders foresee more pain for the precious metal.
"Once we broke that support at $1,418, you have to look at that as resistance," said Anthony Grisanti of GRZ Energy. "If we settle below $1,400, we're going to take a shot at $1,300."
It ultimately comes down to the dollar, according to iiTrader CEO Rich Ilczyszyn. "The dollar is the tallest midget in the room right now," he said on "Fast Money: Halftime Report." Because the currency has been strong relative to others, gold has suffered from the lack of inflation, the trader said.
"For years, gold was a great hedge against purchase power, and now that comes into check," Ilczyszyn said. "Gold will continue to go down until the price is attractive against stocks," he wrote on CNBC.com.
Gold, stocks, and the dollar are more intertwined that it initially appears, according to George Gero, RBC precious metals strategist. In a Wednesday morning note, he wrote that "the recent decline in gold prices is a result mainly of strong stocks using up dollars, as international investors participating in the 14 percent up move this year in stocks have to pay for them with dollars."
That would mean that the rally in U.S. equities makes gold less attractive for two reasons: One, it has strengthened the dollar; two, it has made gold a major relative underperformer.
How far can gold drop?
"If we get through the $1,300, gold can hit $1,100," Grisanti said.
On Tuesday, gold dropped below $1,400 for the first time in a month. That aligns pretty well with what Fortress Investment Group President Michael Novogratz told "Squawk Box" on Tuesday morning: "Gold's bubble has burst," he said. "You've tried everything you can to make it go higher and it won't go higher. Therefore, it will go lower."
Is the market's run done, or is there 40% more upside from here?
According to RBC U.S. Market Technical Strategist Robert Sluymer, the answer is "both." It just depends on an investor's time frame.
Sluymer said the market is a bit overdone in the short-term, but over a three-to-five year horizon he sees the S&P 500 taking 2,300 to 2,400.
How does he arrive at that target? Simple math.
As we head into Memorial Day, drivers are experiencing the lowest gasoline prices since 2008. So what explains the weakness?
Refiners are running strong, strong demand for gas has yet to materialize, and supplies outside of the Northeast are good. Couple that with weak numbers out of China on Monday, and you can see why demand for crude will be lagging.
I do not foresee low prices all summer long. After all, demand is expected to increase, and refiner problems are always at risk of cropping up. But by the same token, I do not see $4 gas on the horizon. Sure, we might get a few pockets around the country, but overall, it looks like smooth sailing (or driving).
So what levels am I looking at?
On the downside, I would buy at the $2.75 to $2.70 level. Indeed, gasoline has found solid support here. And as far as the upside goes, I would sell out of my position at $2.90.
Gold bears want to see a close below $1,418 Monday.
Gold sold off on Friday, reaching the lowest level since April 24 ahead of May options expiration. All week, gold was hitting a wall just below $1,480, and after reaching stops below $1,455, it picked up momentum to the downside and broke the major $1,437.50 support level.
The low on Friday was $1,418.50; however, the market found support against a trend line and was able to rally into the close. Gold reached $1,436.60 upon the floor close, and surprisingly reached the major $1,447 pivot level upon the electronic close, putting shorts on edge as the market failed to follow through.
Gold dropped three percent Friday, suffering the worst decline of any widely followed commodity as it plunged to a two-week low.
Many experts blamed the weakness on the U.S. dollar. The Dollar Index, which compares the dollar to a basket of foreign currencies, has risen nearly two percent over two days, to levels it had not seen in over a month. A rising dollar tends to hurt gold, because it makes gold relatively less expensive in dollar terms.
That's how George Gero, precious metals strategist at RBC, explained it. "Dollar moves were attracting sellers to gold as a strong dollar priced gold higher," he said.
But Friday fear is also hurting the gold market, Gero said. "Short-term investors unwilling to hold positions over the weekend left sellers with few buyers, so support levels have been breached," he wrote in a Friday note.
On the other hand, Carter Worth, Oppenheimer's chief market technician, said the chart tells you all you need to know. "A multiyear bull market has transitioned to a bear market," he told CNBC.com. "The backing and filling of late is the normal setup for the next leg down."
In other words, the tight range that gold was trading in was a precursor to the selloff—and it's nothing he hasn't seen before, Worth said. "After an initial plunge, and then a partial snap-back, any instrument usual goes quiet ... only to drop again, resuming its downtrend," he said.
CME Group brings buyers and sellers together through its CME Globex electronic trading platform and trading facilities in New York and Chicago.
Take your trading to the next level with a platform that lets you trade stocks, options, futures and forex all in one place with no platform or data with no trade minimums. Open an account with TD Ameritrade and get up to $600 cash.


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Noise music is a type of music that employs noise as a musical resource. It is generally created with nontraditional musical instruments such as static, feedback, electronics, circuit bent instruments, or any other noise source. The genre explores cacophony, repetitive sounds, atonal randomness, indeterminacy and dissonance. The earliest noise music was created by futurist composer Luigi Russolo in the early 1910s. His piece "Veglio di una città", released as a single in 1913, was the first ever noise song, shocking Italian audiences.
Full of Hell
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Obsidian Kingdom
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Tagged: Black Metal Noise Experimental Black Metal Raw Black Metal Drewgaze


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Tim DeChristopher transferred to Utah halfway house
SALT LAKE CITY — Environmental activist Timothy DeChristopher was transferred Wednesday from federal prison to a halfway house in Salt Lake City, according to his attorney.
DeChristopher, who inspired a national following by posing as a bidder to protest a December 2008 Bureau of Land Management auction, arrived in Utah by train.
"He's most anxious to see his friends and supporters," defense attorney Patrick Shea said.
Following his conviction at a March 2011 trial in federal court, DeChristopher was given a two-year prison term. He spent time in Colorado and later was transferred to Northern California, where he was housed at the Herlong Federal Prison.
Last month DeChristopher lost his appeal before the 10th Circuit Court of Appeals on convictions for violating provisions of the onshore oil and gas development leasing act and misrepresenting himself as a bidder.
No further appeals are anticipated, Shea said.
DeChristopher, a University of Utah economics student in 2008, had planned to join protesters outside a BLM auction in Salt Lake City, where multiple parcels were on the table for potential oil and gas development. Instead, he went inside, registered as a bidder and was given a placard, No. 70, to raise for bidding on parcels.
The activist ended up winning more than a dozen parcels valued at $1.8 million, though he lacked the money to pay for them. DeChristopher has said too many of the parcels were located in scenic vistas and he bid on them to protect the planet from climate change.
Although his attorneys tried to raise the legal argument that DeChristopher acted out of necessity to protect the environment, that defense was shot down and climate change never became a issue in his legal case.
DeChristopher will be eligible for release from the Salt Lake halfway house to home supervision in February, Shea said. In April, he'll be placed on supervised release, the federal system's version of probation.
Shea said his client intends to remain involved in the environmental movement, to the extent his probation will allow.
"He's still very dedicated to the principles and will continue to be an activist," Shea said. "But he's going to have some restraints from the (U.S. Probation Office)."
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Images of Joe K. Dash(0 from 0 )
Telephone & Addresses
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Joe D Dash (Age: 73)
10032 New York NY
Joe Dash
11215 Brooklyn NY
Joe Dash
19107 Philadelphia PA
Joe Dash, Allston, US, Allston St
View Joe K.'s social profiles and photos on Facebook, MySpace, and +40 Networks.
Yellow Page contacts
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lastFM: Joe Dash – Sons Of Nice Young – Listen and discover ...
Joe Dash – Sons Of Nice Young at Last.fm. People who like Joe Dash also like Forward Edge, april & the fools, Intestines. Discover more music, concerts, videos ...
lastFM: Joe Dash's Biography – Free listening, videos, concerts, stats and...
Top tracks from Joe Dash: Cutdown, Surgery & more. People who like Joe Dash also like april & the fools, Intestines. Discover more music, concerts, videos,...
lastFM: SONS OF NICE YOUNG – joe dash – Listen and discover music at Last.fm
SONS OF NICE YOUNG – joe dash at Last.fm. People who like SONS OF NICE YOUNG also like クボタタケシ・ワタナベトシミ, Nathalie Wise, EL LATIN SWINGERS. Discover more music,...
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Joe Dash | DASH'S MARKET | Email, Owner, @
Joe Dash is the Owner of DASH'S MARKET. Lead411's profile includes an email address, @, linkedin, biography, etc
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LinkedIn: Joe Dash | LinkedIn
View Joe Dash's professional profile on LinkedIn. LinkedIn is the world's largest business network, helping professionals like Joe Dash discover inside ...
Joe Dash - Model Mayhem
Joe Dash. Model Male Melrose, Massachusetts, US. My Website: Joe Dash. My MM URL: http://www.modelmayhem.com/joedash. Mayhem # 1014094 ...
Joe Dash | Dash | ZoomInfo.com
View Joe Dash's business profile as Owner at Dash and see work history, affiliations and more.
Joe Dash - Buffalo, New York - DASH MARKETS
Joe Dash Is A Person Who Works At DASH MARKETS Markets & Supermarkets
Private Homepages
Future Website of joe-dash-whalen
Are you the owner of this site? Building a fantastic website doesn't have to be hard. Succeed Online. You can have a professional-looking, easy to manage ...
classmates: Joe Dash
Strong Vincent High School, Erie, PA, 1985-1989
Celebrities & Politicians
Joe Dash Discography at Discogs
Compilations. Cutdown and 1 more… Various - Messthetics #108 - South Coast D.I.Y. '77-81 (CD, Comp, Enh), Hyped To Death, 2011. Reviews & Discussion.
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findagrave: Dash, Joe
01/15/1880 - 10/20/1926, Colleton County, South Carolina
Mary Dash Obituary - Erie, Pennsylvania - ObitsforLife.com
Mary is survived by her three children, Brenda Eggleston and her husband Rodman Jr., Brian F. Dash and his wife Yvette and Joe Dash and his friend Sue Zuba ...
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Joe Dash (deceased) - Genealogy - Geni
Genealogy for Joe Dash (deceased) on Geni with over 140 million profiles of ancestors and living relatives. Page updated July 3, 2014.
Person Details for Joe Dash in entry for Lawrence Martin ...
Joe Dash in entry for Lawrence Martin and Laura Dash, "Virginia, Marriages, 1785-1940". Go To: PRINT; SOURCE BOX. Name: {{ person.personBestName }}.
Joe Dash (1893 - 1969) - Springfield, MA
This is a profile of Joe Dash's life with Joe's family tree and family photos. Joe Dash was born on August 14, 1893. Joe Dash died in October 1969.
Joe Dash
Potential photos and documents for Joe Dash. View All 6 Images · Joseph Dash. Joe Dash · Sarah Josephine Dash. Joe Dash · Etta E Dash.
Books & Literature
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Along the Cherry Lane - Page 147 - Google Books Result
As we were at the door, Joe Dash said to Alan and me, "I hope Dick knows what he's doing; that's a very rich deal." At that, Alan got very angry. He said, "Joe, if ...
Billboard - 1 Sep 1979 - Google Books Result
Thanks lor your understanding. work almost weekly," confirms Joe Dash, CBS vice president of business development working closely on the new digital ...
Billboard - 11 Mar 1972 - Page 61 - Google Books Result
To push its concept at the dealer level, Kavan has established a task force — Ron Bledsoe, Joe Dash and Jerry Budelman — to "talk SQ at every level of the ...
Billboard - 14 Nov 1981 - Google Books Result
Joe Dash, chief ol Mastei works, was invited by Domingo lo atlend a performance of "Talcs Of Hoffman" in Covem Garden Dash recalls he didn't realise, until he ...
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Joe Dash Music Links - Setlist.fm
Get Joe Dash setlists - view them, share them, discuss them with other Joe Dash fans for free on setlist.fm!
Boottee Lyrics - Benzino
Joe Dash but I'm tryin to make that ass mine. Player always on the grind scopin some take home. Creepin, bonin, see you in the mo'nin [Chorus - Teddy Riley]
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Dominik Joe Dash - Houston, TX
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Crimson Joe Dash. 51 Connie Buck. Sheza Sonny Command. 52 Jesse Pinkerton. Stitch. 53 Ali MacCarty. Dessy. 54 Amanda Johnson. Billies D Copper.
JOE DASH's (DASH113) Pictures, Photos & Images | Photobucket
View and follow JOE DASH (DASH113)'s photos and videos on Photobucket.
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Joe Dash 2013 Acting Reel - YouTube
► 3:14► 3:14Published on March 16,2013 Excerpts from: The Dark Places (2012) The Original Party Trolley (2012) In ...
Joe Dash Dramatic Reel 2 on Vimeo
This is "Joe Dash Dramatic Reel 2" by Joseph DeGeorge on Vimeo, the home for high quality videos and the people who love them.
Joe Dash: How to use the Escape Latch (Glocal Minute) - YouTube
http://www.globesville.com This week, Content Manager Joe Dash shows us how to use the escape latch inside the trunk. Most modern cars are equipped with a la...
Joe Dash Comedic Reel on Vimeo
This is
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It's A Coo-fest As Quadruplets Turn 1 Babies From ...
Parents, an uncle, a sister and cousins adhere to a somewhat stricter schedule, coming midweek or on Fridays, when Lisa and Joe dash out for ...
Dash's Market brings freshness and quality to Buffalo-area ...
WILLIAMSVILLE, NY -- Somewhere, Joe Dash's grandparents are smiling. Mr. Dash's grandparents Mary and Joe, who ran a small produce ...
Dash's Markets gives back to community - The Amherst Bee
Mark Mahoney, director of operations for Dash's Markets, left, and Joe Dash, owner, display some of the fresh produce for sale at 499 West ...
Dash's celebrates 90 years, adds to scholarship program ...
"We all know those people whose efforts are overshadowed by others in more high-profile positions," Owner Joe Dash said. "We want to ...
Reports & Statements
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joe dash | Tumblr
Hi Guys ! Are you getting tired of my love yet lol Well this is going to be the second part of my Gift/Love you Series ❤. Again, I originally didn't want to do ...
i'm feelin it, billy joe dash!!!!!!!! - Tumblr
And heeey pal! Pal-io-buddy-o-mine if you're asking a question that you think i've probably already answered, check my faq or my question tag.
Joe Dash (iknow_joe) on Instagram | iPhoneogram
COMMENTS 10. 1374516758iknow_joe. Hahaha im an adrenaline junky too @habeshapapi we gotta get u onboard next. 1374517099habeshapapi.
Kevin's 336: Wikipedia: Dash's Market
Joe Dash, the current owner and operator of Dash's Markets is a third-generation grocer. The first Dash's store was opened by his father, Frank, ...
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"Joe Dash" Chords and Tabs - Riffstation
Get chords and tabs for any "Joe Dash" song in the world synced with the YouTube video. See "Joe Dash" tabs for guitar, tabs for ukulele and chords for piano.
Food for thought: Joe Dash - Buffalo - Buffalo Business First
"We took a new brand on the market 11 years ago," Joe Dash said of reintroducing Dash's to WNY. "There were a lot of barriers to get through.
Joe Dash - Quazoo
Collection Zone. http://assets.bizjournals.com/buffalo/print-edition/. Joe Dash. Tree Zone. Connection Zone.
Joe Dash Reports from the NYIT OW Campus BBQ
The man, the legend Joe Dash, Globesville's Content Manager reports this week from the quad for the NYIT Old Westbury BBQ in his Glocal ...
ModelClub Inc. - ModelClub Inc - Joe Dash
Bio. Hi, my name is Joe Dash. I grew up in Boston, Massachusetts. I am of Italian and Irish descent. When I was young I dabbled in acting doing such roles as ...
Mr Joe Dash Quarter Horse - All Breed Pedigree Query
Pedigree for Mr Joe Dash, photos and offspring from the All Breed Horse Pedigree Database.
JOE DASH > Similar artists - YouZeeK
JOE DASH, - Play: Sons Of Nice Young, Truth About Surgery, Play Top Albums, songs, Concerts, Biography.
Joe Dash - Awwwards
Joe Dash. Joe Dash has not yet updated their profile. 35 FOLLOW · schema.tc VISIT SITE · Joe Dash has voted for this site. 9; 9; 9; 10. 1 year ago ...
punkbrighton | The Bands | Joe Dash
About Joe Dash. Shaun Jones • vocals, guitar, keyboards. Jon Goode • guitar. Dave Godot • bass. Paul 'Ludo' Martin • bass. Mark Leechasko • drums
Joe Faruq Dash's stream on SoundCloud - Hear the world's ...
Joe Faruq Dash. Joe Dash Savage, United States. 13 followers13; 1 track1. Follow Share. Joe Faruq Dash. Play. Re:search · Just dabbling with what I have--.
Joe Dash's Website d(^^d)
Joseph Dash. J as in Joseph Dash. O as in OMG its Joseph Dash. S as in Somebody loves Joseph Dash. E as in Everybody loves Joseph Dash.
@morbidqueen21 #Joe Dash En Instagram Photos ...
Joe Dash En morbidqueen21 Instagram Photos : morbidqueen21 4 1 16:38. Ang jubis lang! morbidqueen21 6 0 15:35. Happiest Birthday to my ...
Anishinaabe legend, The Woodpecker - Anishinaabemdaa
"Hey Joe" dinaan saa giiyenh Joe dash giinkwetwaan "Waa" dinaan "Giikendaan naa kego". "Wegnesh" kida saa giiyenh Joe. "Gwanda binenhshiinhak ...
Dash's makes a dash for Kenmore Ave. | Hello Hertel
Contact Joe Dash directly-. http://dashsmarket.com/dashs/contactus.jsp · Reply ↓. Raelyn on December 2, 2013 at 1:43 PM said: This is such ...
Fatal Fury (Neo Geo) - The Cutting Room Floor
US/Europe, Japan. Fatal Fury (Arcade) Joe Dash US.png, Fatal Fury (Arcade) Joe Dash JP.png. Finally, the command for Joe Higashi's "Slash ...
Joe Cavanaugh's Homepage - MyWeb - University of Iowa
Office: N312, College of Public Health Building Office Phone: (319) 384-1602. E-Mail: joe dash cavanaugh at uiowa dot edu. FAX: (319) 384-1591. Address:
Next Self-Storage Facility Planned for Former Budwey's on ...
Joe Dash has some ideas for the former Budwey's Supermarket at 416 Kenmore Avenue and it's not what neighbors had hoped for or expected ...
OPINION: Why a grocery store on Hertel Avenue matters
This week, the neighborhood received more bad news when we learned that owner Joe Dash will not only close the store on Hertel, but that he ...
RUSHOUT - Rakuten.com
RUSHOUT: W28 ☆ old clothes leather underwear JORDACHE Joe dash black /14may07 - Purchase now to accumulate reedemable points! | Rakuten Global ...
Residents angry local grocery store closed | wivb.com
Joe Dash, the owner of Dash Markets, said due to construction on Kenmore Avenue and low demand for a supermarket, it's not financially ...
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