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Forums > Critique > Serious Critique > Serious critique wanted!!
Posts: 33
Antrim, Northern Ireland, United Kingdom
I have started getting back into modelling this year id like everyones thoughts on my old work and also my new..i want to hear some constructive criticism to push me in the right direction when it comes to bettering by preformance
Emma x
Nov 12 12 02:52 pm Link
Posts: 3966
Lansing, Michigan, US
I suppose your 'new' work is the images with less than about 100 views, and your 'old' work is those images with over about 100 views. Sorry, I ain't gonna go looking at posted dates to sort them out.
Looks pretty much the same to me, the exception being the first image in your portfolio. It's interesting in a general pin up way. Is it a WOW! Nope... but it ain't bad either.
I'm of the opinion that model's should learn as many different genres as possible if they want steady work (and pay...). So practice up on pin up poses... Look at Gil Elvgren and other pin up artists work for samples of poses. Most modern illustrators are doing warrior hero stuff so classic pinup is left to photographers for the most part.
Just my opinion.
Nov 12 12 06:47 pm Link


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Laura Limmitt
- 39 years old
- Date of birth: Dec 10, 1975
- Date of passing: Jul 31, 2015
|Let the memory of Laura be with us forever
This memorial website was created in memory of our loved one, Laura Limmitt, 39, born on December 10, 1975 and passed away on July 31, 2015. We will remember her forever.
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"Very hard sometimes to get up in front of people and tell them what you meant to me . I could talk for days about that if I had to . The memories I share with you will always be in my heart, I will always love you."
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Back home!
Santee Travel Blog› entry 8 of 8 › view all entries
Today was the day to return to reality. I had booked a airport shuttle to pick us up. I went online and found one a bit more reasonable than the one suggested by the hotel. The driver picked us up (on time) and started for the airport. We had to make one more stop, down a very long and narrow alley. I was impressed when the driver backed his way all the way back down the driveway...don't think I could have done it with out leaving some paint on the walls.
When we got to the airport we had to check in our luggage and the wood sword and the large fan that the boys had purchased in Chinatown. You should have seen the face of the staff in the terminal when we walked in with my son carrying the sword. I am sure that had I been carrying the darn thing, I would have been proned out and subject to all sorts of embarassing bodily searches. As it was, the young man that was working the check in counter was having a blast playing with the sword before he finally wrapped it to put on the plane. The boys and I finished our check in and head to the assigned gate. But life could not possibly be that easy. About 45 min before we were scheduled to board, they called a gate change...all the way at the other end of the terminal...ARGGG!!! Then when we got to the new gate, I looked at my boarding passes more closely. I noticed we had not been assigned seats. I checked with the guy working at the counter and asked him about it. I asked if the flight was kind of cattle call type thing like Southwest Airlines. He looked confused and checked this screen and checked that screen, and made some comments I did not quite catch. I told him I was flying with 2 kids, and unless he wanted them to run amuk (not that they would, they are well behaved) we probably should all sit together. Well it all worked out, and we got to sit together.
Note: When we were on approach in San Diego, my son looked out the window (Brian again. I swear the kids does not miss anything) and told me he could see the neighborhood where we live. Our neighborhood is not really under any regular flght paths so I told him, No way. I looked out the window and he started pointing out landmarks around our house (Santee Lakes). He than told me he could see his school. I laughed when he told me he could tell where his school was because he could see all the dirt from the athletic fields... take note those of his teachers that may be reading this :)
Well, we got into the airport and caught the shuttle to the long term airport parking. This was the first time I had used this. I think it worked out rather well, and will probably use it again.


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Father: Jacob Douw FONDA b: 19 OCT 1793 in New York
Mother: Cornelia Anderson SCUDDER b: 16 MAR 1803 in Freehold, Monmouth Co., NJ
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text: Zoom Tiddly Winks Availability: In stock £8.50 Product Description Additional Information Product Reviews Product Description Details Children will love playing this traditional game of skill. Perfect for playing with friends, family or alone, children will enjoy popping the 'winks' into the pot while learning key skills in dexterity, coordination and strategy along the way. Great for popping in a bag or pocket and taking on trips away. A classic game that never goes out of fashion. Suitable Age: 3 Years + Additional Information Additional Information Age 5+ Learning Value Fine Motor Skills, Physical Development Product Reviews Write Your Own Review You're reviewing: Tiddly Winks How do you rate this product? * 1 star 2 stars 3 stars 4 stars 5 stars Quality Price Play Value *Nickname *Summary of Your Review *Review Loading... Enter security code Refresh captcha Submit Review CMS tab Options Gift Wrap +£2.50 £8.50 Qty: Add to Basket Add to Wishlist | Add to Compare Tweet Email to a Friend Add to Wishlist | Add to Compare You may also be interested in the following product(s) Storyonics - Creative Story Game £6.50 Tic Tac Toe Book £15.50 £8.00 Shut The Box £10.95 £8.00 Dude Dice Level 1 £6.95


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… peanut butter,
you make my heart flutter
I was at Cobb's Comedy Club last week and Shawn Wayans compared cheating to Chinese cooking. He said he didn't get why people kicked such a big fuss when their partners cheat. Usually, when that happens, couples are at that stage where they don't even talk or have sex anymore. That partner is basically like "old Chinese food," he says. It's been in the fridge forever, but after someone throws in some soya sauce, stir-fries it, makes it new and smell all good again, you look at that person and say, "Hey, that's my Chinese food." LMAO.
Anyway, his joke reminded me that I had some teriyaki chicken and lettuce leftover from lunch at Sushi Nara on Polk Street a couple of days ago. If I didn't do something with them, they would soon go bad.
Have you ever had a hug so tight and so warm it made you feel all fuzzy inside?
That's how eating a plate of well-made Risotto Ai Funghi Porcini makes me feel — like every mouthful is a hug that makes you feel so warm and loved that you want to hug the person who made it for you. That's probably the reason I wanted to learn to make it — I crave carebear-type love! LOL.
The recipe itself seems straightforward enough; first, you cook the mushrooms, then you cook the rice. In the final step, you throw both together and voila, you have a slightly nutty, slightly glutinous plate of chestnut brown goodness. Unfortunately, in this case, the cliche — the devil's in the details — is very true.
Boletus edulis. Penny bun. Cep. Or otherwise, porcini. The Italian language, with its singsong quality and highs and lows, has a way of bringing to the surface the character of the thing it is describing.
Porr. Ch. Nee. There is something fun and cheeky about the crescendo and staccato stop of each syllable, yet the experience of the entire word brings a seriousness and fullness of purpose — as if the mushroom is a unique individual in its own right and its name validates that uniqueness.


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Crossings at DaybreakWalk Score: 34
4950 W Frogs Leap Dr South Jordan, UT, 84095Check your credit here before your landlord does Check your credit here before your landlord does
Get away from crazy city life to come relax in the luxurious living at Crossing at Daybreak Apartments in South Jordan, UT. Our irresistible property, Crossing at Daybreak, is conveniently located close to the new highway, Mountain View Corridor, and the UTA Trax station, ensuring your commute to work easy and comfortable. Also next to the popular Oquirrh Lake, with over 22 miles of hiking, biking, and walking trails within a 5 minute walking distance of your apartment. Daybreak offers special amenities such as soccer fields, volleyball courts, community gardens, movie nights in the park, and many more in a top Utah Apartment.
As the only apartment complex in Daybreak, UT, Crossing at Daybreak offers 1, 2, 3 bedroom floor plans with high end finishes and life's most desirable features. We offer all the amenities you're looking for; spacious granite counter tops, large walk in closets, full size washer and dryers, private patios and balconies with breathtaking scenic views, and who wouldn't love a warm fireplace to cozy up to on those cold winter nights!
At Crossing at Daybreak Apartments, you'll enjoy friendly and responsive staff members along with a quick response time to any maintenance request. You'll love our inviting community that has everything you need; 24-hour fitness center, relaxing pool and spa, professionally landscaped grounds, detached garages, ample extra parking, a large playground, and the pleasure of the close shops and dining at Soda Row and The District. Let us take you on a tour of this master planned community you'll love to call your new home!
*Pricing and availability are subject to change.
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Tackling the 'Water Problem': Challenges facing U.S. regulation, sustainability, and global geopolitics
Past Event
Tackling the 'Water Problem': Challenges facing U.S. regulation, sustainability, and global geopolitics
Tackling the 'Water Problem': Challenges facing U.S. regulation, sustainability, and global geopolitics
A conversation with former U.S. Secretary of Interior Bruce Babbitt
U.S. water infrastructure and regulatory reform
Climate impacts on water are felt at every corner of the globe. From drought in California and a shrinking Colorado River to limited access to safe and clean drinking water in emerging economies, concerns about water are increasingly urgent. Addressing the water crisis facing the United States and other nations requires innovative, bipartisan ideas for how to alleviate water challenges, bolster resource security, and foster sustainable economic growth.
On March 28, the Cross-Brookings Initiative on Energy and Climate and the William S. Boyd School of Law at the University of Nevada, Las Vegas, hosted a half-day conference on key issues in water policy. This event celebrated the launch of the new Brookings Press book "The Water Problem: Climate Change and Water Policy in the United States," edited by UNLV/Brookings Senior Fellow Pat Mulroy.
Following an opening keynote conversation from former U.S. Secretary of the Interior Bruce Babbitt, Mulroy moderated a panel discussion on domestic water infrastructure and regulatory reform. A second panel of experts analyzed issues facing global water security, economics, and geopolitics, including regional dynamics in the Middle East, Southeast Asia, and China.


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Hello friends,
Which tool is best for checking website Problem?
iwebchk is the best tool to audit your website.
Depends what the issue is!
Are you talking about anything specifically or just a general tool for example SEO Audit?
My suggestions is SEO site checkup and Small SEO tool is best.
Open site explorer is best Moz SEO too to check site problems.
AS Deepak said open site explorer is a best tool and there is also a Google Webmaster which keeps track of all site data
Ahrefs is the best tool for site checkup.
Small SEO tool is best.
**Links removed by Site Staff so it doesn't look like you're spamming us. Please don't post them again.**
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Hi there,

Do you know a good gym within Guomao?

Thanks!

Alex

I'm going to try to get a membership with a gym sorted tomorrow in either Shuangjing or Guomao, so I'll let you know what I find. If anyone else has suggestions I'd be more than happy to hear them.
Let me know too!!! I need a gym with weights.
Otherside of WanDA there have a nice one ,,


text: Shivrajkumar-Sonal Chauhan starrer Cheluvine Ninna Nodalu has completed nearly 50%of the shooting; recently the climax song was shot at Chancery Pavilion under dance choreographer Imran Sardaria. All the important actors in the film (Shivrajkumar, Sonal Chauhan, Tarun, Venkatesh Prasad, Dattanna, Vinayaprasad, Chitra Shenoy, Doddanna, Sangeetha and Shanthamma along with a Russain ballet troupe and 40 African dancers) took part in the filming; the song was shot at a cost of Rs. 60 lakhs.In CNN, Shivrajkumar plays the role of a tourist guide who has excellent knowledge of the New Seven Wonders of the World. In March, the team will fly to the all the monuments which are in the list of the New Seven Wonders of the world for filming. Debutant Raghuram handles the story and screenplay apart from direction. V. Harikrishna is the music director while cameraman Kabir Lal is returning to Kannada after a long gap.


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Last month, I went to San Pedro, Belize and met up with three of my best friends for an awesome girls' vacation. We ate delicious food. We drank fruity, blended things. We flirted with beautiful men. All in all, it was exactly what a girls' vacation should be. Plus hammocks!
San Pedro, an Introduction
You will fall in love with San Pedro. Everyone does.
San Pedro
The city is located on the island Ambergris Caye. This, along with Caye Caulker, are the two islands you hear about most frequently on travel blogs.
This advice pretty much sums up my experience as well:
If you're new to the island, leave about two thirds of your clothing at home, bring swim suits, sun block, sandals, and a hat. I struggled hard to find a single evening I could wear long pants in an entire two week stay. This is one very relaxed place. Most people step off the plane, and struggle for about three days to slow down. It's hot and fragrant and moist, the wind is cooling, and, well, things just don't seem so urgent.
Getting There
We flew into Belize City, took a cab to the Water Taxis, and the Water Taxi to the island. This is a pretty common way to get there, and we were following the advice of our AirBnB hosts. There is a small airport on the island, so you can fly in directly if you choose.
Things to Do
There's a lot to do in a town like San Pedro, and you can't get to all of it in a week. Here are seven of my favorite things we did while there:
Walking the Beach Path & Going for a Swim
The town is longer than it is wide because of the geography of the island. There's a beautiful path that's lined with the Caribbean on one side and hotels and businesses on the other. There's the occasional bar, but not many. There are a few spots along here that are good for a swim. Even if you're not planning on going into the water though, walking the path is a beautiful way to spend some time while you're in town.
Tooling around on a Golf Cart
Not an activity per se (although they list it as one here), just a damn good mode of transportation. San Pedro is small and there aren't tons of cars. Tourists and locals tend to hop around on golf carts. We rented one for 5 days for $200, which for us was $10 per person per day. You can walk San Pedro without one (though I wouldn't too late at night) and there are a good amount of cabs, so it's not a necessity. But it was awesome to have for convenience. More than that though, it was super fun!
I mean, isn't it every little kid's dream that their parents let them drive around in a golf cart? It was for me.
We rented ours from a local company called Avis (not THAT Avis), and got rates way lower than the published ones by asking. San Pedro isn't super-haggley, but you can always ask for a better deal.
Hanging out at Secret Beach
Question: How NOT a secret is Secret Beach?
Answer: There's a taco truck. DJed tunes. A restaurant. A palapa. A second pier. Bathrooms. Wifi. And multiple signs pointing to its location.
But it's still awesome. So awesome, we went back a second time.
Getting there is kind of a chore, which is why it's kept its name. So even though everyone around you knows all about it, it's remoteness keeps it more chill. Especially on weekdays during the off-season. A cab out there is about $50 each way, which is another handy reason to have a golf cart around, at least for a day or so.
When you get there, you'll have the aforementioned amenities, although you won't see much civilization on the way.
You'll also have crystal-clear blue water that's standing depth for a long ways out with views of mangrove islands in the distance.
I wrote about my experience snorkeling with the Pro Dive Center for this piece on the Belize Barrier Reef System. This was an activity we added on the tail end of the last day, but in a lot of ways it made the trip.
There are tons of other water adventure activities available as well. If that's your thing, Belize can make it happen.
Trying Delicious Things at the Belize Chocolate Company
Local chocolate. Turned into a milkshake. Drank while staring at the Caribbean.
There's literally nothing about that situation that isn't perfection.
Relaxing at the Beach Bars
Up and down the island, there are a number of chill beach bars that have good tunes, incredibly nice bartenders, and delicious (if a little pricey) fruity mixed drinks of the sort that a good vacation demands. Some also have food. We spent a lot of time at the Dive Bar because it was so close to us, and because the staff was so wonderful. We also went to Pirate's Treasure and Wayo's Beach Bar. None of them disappointed.
Enjoying the local Taco/Yoga/Massage Spot
The Ak'bol Yoga Retreat and Eco-Resort is a little north of town, but worth the drive! My friends went for the yoga, but stayed for the tacos. And then went back. And then we all went back two more times.
They also have massages. And cabins to rent. And a friendly dog. And a really hot waiter, to be honest.
The food here was by far our favorite we went to. I ate the fajitas for dinner with tons of guac. Then for breakfast, I had eggs and guac and salsa. They had pancakes. They had so many kinds of tacos. All four of us on the trip couldn't stop talking about how good the food was. And less expensive than most of the other restaurants in town.
Because it's San Pedro, you're also eating these delicious things (or doing the endurance yoga) by a pier with palm trees everywhere. Like a freaking Gaiam workout video in real life. But with tacos.
Other Tips
- This is a good travel guide if you're looking to pick one up before your trip or on your Kindle while you're there:
- A good hourly/ninety-minute regimen of sunscreen and bug spray (with DEET!) will go a long way. Skip this even once, and you'll be sorry. Try to bring some with you, as these are pricey.
- Go inside at dusk. That's the worst time for the mosquitos.
- The off-season has the potential for hurricanes, but the town is a lot emptier with basically the same weather an amenities. Slightly better prices.
- I wasn't trying to be too budget-conscious on this girls' week extravaganza, but if you want to do the island on a budget, check out this great post from Eternal Arrival on how to do it as a budget destination.
- The Water Taxi is not exactly like a pleasant boat ride. I had a mixed experience. On the way over was terrible and we kept hitting the water so hard it felt like cement. The way back was just crowded and a little hot.
- Prices are not as low as on Caye Caulker. We cooked a lot of meals to balance the meals out.
- It's an island, so everything is imported. If you want to save money on alcohol, bring a bottle from Duty-Free for your home supply.
- You may need a day or two to adjust to the humidity and heat. Take the first day easy if this is the case. The more you can be in the water, the cooler you'll be.
Have you been to San Pedro? Got any tips or stories? If so, share below!
Pin Me


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I have been on the search for shapewear that I could wear every day. The two major features that were important to me were comfort and that it would stay in place all day. Luckily, I discovered an amazing new line called Ruby Ribbon shapewear. What I love about the company is that besides just making traditional shapewear, they also make clothing with shapewear built right in!
I needed an everyday essential. So, i decided to give the Classic Cami a try. After having a baby the main area I wanted smoothed out was my stomach. I loved the results! I step into the cami and pull it up to ensure it seals everything in and stays put all day. After I put my clothes on, I could immediately tell a difference in the way I looked and of course I loved it. My mid section looked very sleek; something I hadn't seen since before I became pregnant with my little one!
Are YOU ready to Shape Up? Shop ruby Ribbon's Traditional shapewear HERE:
Or shop Clothing with shapewear built right in HERE:
Happy Shopping!
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2 Bedroom Apartment in Uvongo
Hettie du Plooy
Rawson Property Group-Hibiscus Coast Uvongo
2 Bedroom Apartment in Uvongo
R 860 000Can I afford this?
Neat top floor apartment features an open plan kitchen, the lounge leading onto a patio. This property sold fully furnished and is situated in the most popular complex on the south coast which offers excellent holiday letting.
The Complex is a full of entertainment with a tennis court, 2 communal swimming pools with braai facilities and change rooms. The one swimming pool is only for owners and situated near the waterfall. The complex offers outside braai facilities, 2 laundries, a supervisors office and 24 hr. security
Uvongo is known for its large rock escarpments thick with beautiful foliage which shelf's the Uvongo beach. The beach offers shark nets and life guards, ablution facilities, change rooms, a children's paddle pool which is situated on a grassy bank shades by trees, a tidal pool and a small pier for those for those who love fishing.
Key Property Features
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Experience Margate
If you're an avid fisherman, surfer or just someone that loves glorious beaches, coastal forests, magnificent coral reefs and endless sunny days, then the South coast is the place for you. The heart of the KZN South Coast, from Shelly Beach to Ramsgate, has a vibrant atmosphere yet very laid-back lifestyle. The area boasts an abundance of leisure and sporting facilities. People go to work dressed very casually, residents are surrounded by holiday makers, restaurants are always busy and beaches are always attractive.Find out more about Margate
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text:
Choosing a right color scheme is not always easy and it's essential to your website's design. Your layout design including font should be developed in concert with your color scheme, which can ensure readability, cohesiveness
Stunning Orange and Yellow Color Website Designs
Community News
As design junkies, we all benefit from what we put back into the art… So start sharing your latest news, article or resources and tips, by using the submit form. All submissions will of course be monitored and appreciated by the entire community.
3 years ago Community News35


text:
Hawaii '07: Day 4
Waianae Travel Blog› entry 6 of 16 › view all entries
Getting up was tough at 5:20 am, especially after Clint called at 4:30 (he didn't know I was here). But it was a hell of a lot easier than getting up to go to work. I packed my stuff for the day last night so I got up, put on some sunscreen and my bathing suit and headed off to the bus stop. I really wasn't too sure how the bus was going to work out. The bus schedules are kind of confusing but I got the directions off Google so I figured I'd be alright. Sure enough I got there at 8 and had plenty of time to walk to the boat. I was 15 minutes early and got myself situated. The boat was "manned" by two women, Tori (one of the owners) and Ann. The boat was named the Alaka'i, a 34' Baha King cat. Left the harbor a bit late (the people with me were stuck in traffic) but got to a good spot with a big pod of dolphins in no time.


text:
Saudi cement producer, Arabian Cement Company (ACC), has imported 200,000t of clinker from China, Mohammed Taher Othman, ACC director general, was quoted as saying on September 24, 2006.
The imported clinker will be used as a raw material at the company's plant in Rabigh, southwestern Saudi Arabia.
According to Othman, ACC imported 72,000t of clinker from Egypt in 2005.
ACC is currently working on an expansion project at Rabigh plant for the launch of a sixth production line with a daily capacity of 7,000t. The production line is to be operational in early 2008. Published under Cement News


text:
We appreciate you taking the time to visit our website. We are thrilled that you are now interested in making a gift to the School of Information.
You may make a gift using your credit card by using our secure online giving option, or by calling the FSU Foundation at (850) 644-0771.
If you wish to make a contribution with a check, please include a note indicating your gift's purpose. Gifts made by check should be made payable to FSU Foundation and mailed to:
FSU Foundation
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Thank you for investing in the future success of the School of Information.
Our Development staff is ready to discuss your plans for supporting the School, or to answer any questions you may have about the potential impact of your support.
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firstname.lastname@example.com or (850) 645-8312


text:
images via fashion gone rogue
21 January 2011
versace s/s 2011 lookbook
21 January 2011
daphne groeneveld and ginta lapina model the designer's spring and summer 2011 collections in versace's latest lookbook. i personally like the summer collection, worn by daphne, better than spring - the figure-hugging sheath dresses are at once ladylike and sexy and daphne is simply stunning. to see the entire lookbook, click here, and for more details on daphne, check out my september 2010 model spotlight.
5 comments:
- christine {bijouandboheme} said...
Goodness- the shoes!!! The boots in the second shot literally made my jaw drop.
- January 21, 2011 at 7:25 AM
- Emilie said...
aaaarghh love love love this collection!! The dresses are fantastic and I really like the third outfit!! Thanks for sharing :)
- January 21, 2011 at 9:14 AM
- {av} said...
IN LOVE with that turquoise bag. How incredible is this collection! happy weekend! xoxo {av}
- January 21, 2011 at 10:09 AM
- Moosette said...
L.O.V.E. the orange!
Have a fabulous weekend!
- January 21, 2011 at 7:26 PM
- Jillian said...
hola! i tagged you in a post on my blog, play along if you'd like! xo jcd - http://cornflakedreams.blogspot.com/
- January 22, 2011 at 8:42 AM
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London, United Kingdom (PressExposure) December 07, 2009 -- DNA Electronics Ltd, a developer of disposable, real-time gene testing at the point-of-care, has been named as the winner of the prestigious R&D prize at the Elektra09 European Electronics Industry Awards. DNA Electronics, a spin-out of Imperial College London, triumphed from a shortlist of five other finalists to be named winner by an independent panel of judges in recognition of its innovative technology and intellectual property strategy. The Elektra09 award ceremony was held on Tuesday 1st December 2009 at the Royal Lancaster Hotel in London.
DNA Electronicsâ SNP Dr (pronounced âsnip doctorâ) is a breakthrough silicon chip-based handheld device that offers fast and accurate spot test results for specific DNA sequences that indicate how patients are likely to respond to prescription drugs. The device is now being trialed in partnership with global pharmaceutical company Pfizer. Each year, the NHS spends £460 million to treat 250,000 patients admitted to hospital suffering adverse reactions to prescribed medication. A test to identify people likely to react badly to prescribed medication will enable doctors to tailor dosages to the individual needs of patients, bringing greatly improved treatment and massive potential cost savings to healthcare providers.
DNA Electronics was co-founded by the inventor of the foundation technology, Professor Chris Toumazou FRS, who is CEO of the company and director and chief scientist at the Institute of Biomedical Engineering at Imperial College London. The core technology is based upon switching transistors on and off with DNA, which has culminated in the worldâs first DNA logic on standard CMOS technology.
The annual Elektra Awards recognise the achievements of individuals and companies across the European electronics industry. The judging panel said of DNA Electronicsâ submission: âThis was the strongest set of finalists the Elektras has ever seen for the R&D Award. The winner impressed for quality of its basic research in an emerging area of electronics design.â
Commenting on the award win, Professor Toumazou added: âWe are absolutely delighted to have won an Elektra award in recognition of this ground-breaking and important innovation. The cost-effective and convenient testing enabled by the SNP Dr device will allow doctors to assess patients in the GP surgery and prescribe optimal dosages and treatments on-the-spot â without a lengthy and costly laboratory analysis. This award reflects the exceptional talent of the team at DNA Electronics who are now bringing this technology to commercial fruition.â
Please visit the Elektra Awards website for full details of award winners: http://www.elektraawards.co.uk/
ends â
Notes to editors
About SNP Dr The SNP Dr works by analysing genetic variations found in DNA called Single Nucleotide Polymorphisms (SNPs). SNPs are the parts of human DNA that make us all respond differently to disease, bacteria, viruses, toxins or medication. Each year, the NHS spends £460 million to treat 250,000 patients who are admitted to hospital suffering adverse reactions to prescribed medication.
The SNP Dr works by analysing the DNA in saliva or cheek swab samples, which are placed in a cartridge and exposed to the silicon chip sensors inside the device. A copy of the fast or slow metabolic SNPs is contained in the chip. If they detect a match, a message is displayed on the SNP Drâs console. The doctor can then assess their patient in the GP surgery, without a lengthy and costly laboratory analysis, and prescribe dosages and treatments accordingly.


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If yesterday it was just a rumor, today it became official: Audi will bring the TT RS Coupe to the United States market by the third quarter of 2011. Although the introduction of the TT RS was under consideration for several months, Audi launched an innovative survey on Facebook and no more than 11,500 fans expressed their enthusiasm for the Audi TT RS in just one month.
As a result, Audi concluded that it is the time to bring the new car in the United States.
For those who don't know, the Audi TT RS is powered by a 2.5-liter TFSI, five-cylinder engine capable to generate 360 horsepower and an impressive 332 lb-ft of torque, mated to a six-speed manual transmission as well as quattro all-wheel drive.
With these numbers, it can sprint to 62 mph (100 km/h) in just 4.6 seconds and has a top speed electronically limited to 155 mph (250 km/h).
The lightweight body featuring Audi Space Frame ASF aluminum construction produces a low drag coefficient of 0.32. Audi didn't release the pricing for the new car yet. Only the coupe version of the TT RS will be available in the U.S.
Audi of America President Johan de Nysschen revealed that the Audi TT RS remains to be rooted in the brand's motorsport success especially when it comes to performance and even agility. This is the reason why, Nysschen adds, that the TT RS is indeed a sports car that portrays emotion.
This particular emotion was made obvious when the company went around the country to explore any interests on the TT RS, Nysschen concludes. Audi said that it will continue to determine the features and options that it can offer to the U.S. market though it has yet to settle on a final price for the TT RS.
However for U.S. consumers, Audi will only offer the coupe version. Still, consumers should be excited with the fact that the TT RS is considered as the first classic sports car in the brand's RS family. This model was manufactured by quattro GmbH to be a pure driving machine similar to the RS4 and RS6.
The power of the engine is aided with the latest six-speed transmission that allows for a more precise operation due to the newly designed shift lever with its short shift travel. That is not the only thing that the new transmission has to offer however. What makes this one unique is that it manages to narrow down the distribution of its gear ratios and a rather high efficiency ratio.
A standard feature that comes with the TT RS is the quattro permanent all-wheel drive. What makes this all work can be found on the rear axle where a hydraulic and multi-plate clutch that is electronically controlled can be found. The purpose of the clutch is that when the need arises, it redirects most of the torque input from the car's front wheels to its rear.
Thus, in just milliseconds the accumulator works together with the powerful electrical pump to press the plates of the clutch through hydraulic pressure. Another feature that has been added to the powertrain of the quattro is the constant velocity joint. In the TT RS, it was placed before the two heavy-duty components which were the compact rear-axle differential and propeller shaft.
Meanwhile the drivetrain gives it the needed traction, stability, and dynamics. When the TT RS runs through laps or drives through winding roads, it is able to demonstrate its handling ability. The steering of the Audi TT RS is also precise and able to respond quickly.
Then there is the immediate reaction from the chassis and its neutrality at the cornering limit. All of these, with the grip when exiting a curve and the power of the engine, give the TT RS its true value as a sports car.


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Buddy's Auto Repair
Review for Buddy's Auto Repair
Cathy L. on 05/09/2012
Cathy said this business was very nice but she hopes they did not take advantage of her because the check engine light had illuminated. She was told by her son-in-law the light was on because of the fuel to oxygen ratio was incorrect. She will return to the business for resolution tomorrow, or Friday. She would not recommend the business because she found a different, unspecified better mechanic.
Category: Service
Service Date: 05/01/2012
Review Created: 05/09/2012
This review was collected via phone interview by Customer Research Inc.


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Child Development Divisions Childcare Video Magazine: "First Moves: Welcoming A Child to a New Care Giving Environment"
Streaming Media
Asa G. Hilliard, III Papers
Document Type
Date Information
Playing Time
This video recording, dated January 1, 1987, is a short instructional video produced by the state of California's Department of Education, titled "First Moves: Welcoming a Child to a New Care Giving Environment." The recording presents video clips of young children and their parents arriving at a childcare facility while care givers attempt to aid in the transition of the child to the new environment. There is also a panel discussion about the techniques being demonstrated between each set of clips. The video exhorts caregivers to pay particular attention to three techniques: Distance, Indirect Contact, and Time.
File Name
Recommended Citation
Center for Child and Family Studies, Far West Laborartory for Educational Research and Development, "Child Development Divisions Childcare Video Magazine: "First Moves: Welcoming A Child to a New Care Giving Environment"" (1987). Asa G. Hilliard, III Papers, Audio-Visual Materials Series. Recording 40.
All video content in this collection is protected by copyright or is the property of the Robert W. Woodruff Library of the Atlanta University Center, Inc., and/or the copyright holder as appropriate. For more information or to inquire about permission to publish, please contact email@example.com.


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Paul Campanella's Auto & Tire Center
Review for Paul Campanella's Auto & Tire Center
ROSE L. on 05/07/2015
"Knowledgable, efficient and courteous"
Excellant service center. They correctly diagnosed my car's problem with just my description. They are always courteous. Facilities are clean, comfortable. Free coffee! Always a plus. Recommended without hesitancy.
Vehicle: Lexus GX 470
Service Date: 04/13/2015
Review Created: 05/07/2015 05:30 PM
Caroline R. at Paul Campanella's Auto & Tire Center responded on 05/08/2015
Rose, thank you for sharing your experience with us. We are glad to hear that you have been continuously provided with great customer service, for that is what we aim to do! We appreciate your patronage and kind words. Thank you for stopping in!


text: so i jus rooted my nexus one, and i actually paused for a few minutes at the screen where they say the warranty is void...but i was jus waiting for all the goodies at the other side... and i was surprised how market automatically downloaded all my apps for me, and now i can install my apps on the sd card as well! ok..so i got a few questions... i still have the stock 4gb card, but i hope to save up some money and go the whole way and buy a 32gb card! if and when i do change my sd card, is it jus a case of copying the files from one to the other? also, i saw in some threads and other places that to install on the sd card you need to partition it, but i didnt do anything like that, but rather i used ModInstallLocation which is available in the market... with regard to updates, will i get automatic updates now that im rooted?or will i have to go through the whole manual update process? and if i opt to install a custom ROM such as cyanogen, then its just a case of updating the cyanogen each time right?cos then each google release doesnt really matter?and would cyanogen download all my apps for me? thanks in advance guys!


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ZYZZYVA EventsDecember 5, 2017
ZYZZYVA Winter Issue Celebration in San Francisco
Location: 7 p.m., City Lights Booksellers, 261 Columbus Ave., San Francisco
Description: Featuring a conversation with T.J. Stiles and Caille Millner exploring the themes of art and resistance. Moderated by Managing Editor Oscar Villalon. Free.January 12, 2018
ZYZZYVA Winter Issue Celebration in Oakland
Location: 7 p.m., East Bay Booksellers, 5433 College Ave., Oakland
Description: Featuring a conversation with Troy Jollimore, Dean Rader, and Ismail Muhammad on the theme of art and resistance. Moderated by Managing Editor Oscar Villalon. Free.February 15, 2018
ZYZZYVA East Coast All-Stars
Location: 7:30 p.m., Greenlight Bookstore, 686 Fulton St., Brooklyn
Description: Readings by recent and Winter Issue contributors Bino A. Realuyo, Annie DeWitt, Jenny Xie, Melissa Hohl, and Kristopher Jansma. Free.
ZYZZYVA e-mail updates
Tag Archives: Antioch
A Fortunate Literary Community in L.A.: Wendy C. Ortiz and Rhapsodomancy
In Los Angeles, a person can't get anywhere in seven minutes. There's no Muni, BART, quaint Italian streetcar or the tried and true 22 Fillmore. Attending readings can be a chore that involves multiple freeway changes and nail biting, bumper-to-bumper traffic. It's difficult to lure people out for free drinks, a cheese plate, and a discounted literary journal here, where an iffy parking situation can make or break an event. In L.A., I show up to readings because I value the time spent crafting a story, the twenty-four revisions and the manic rehearsals that go into a reading. I know …Continue reading
Posted in News Tagged Andrea Quaid, Antioch, Eloise Klein Healy, Los Angeles, reading series, Rhapsodomancy, Wendy C. Ortiz 1 Comment


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GraphicRiver Girl on the Moon with Bouquet of Roses 8051384
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GraphicRiver Girl on the Moon with Bouquet of Roses 8051384
Stock Vector \ Characters \ People
Person \ Petal \ Pathway \ Magical \ Little \ Plants \ Stairs \ Young \ Steps \ Stem
Stock Vector - Girl on the Moon with Bouquet of Roses 8051384 by interactimages @ GraphicRiver
Stock Vector \ Characters \ People
Graphics Files Included: JPG Image, Vector EPS; Layered: Yes; Minimum Adobe CS Version: CS.
Illustration of a girl going to the moon with a bouquet of roses
A Blue Monster Standing Near the Fence; An Empty Wooden Board at the Farm with Ants; A Happy Family Jumping Across the Lighthouse; A Boy Holding Two Packs of Bean Candies ; Two Smiling Teenagers with Empty Signs; A Monster Holding a Gift with a Red Ribbon ; A Wooden Bridge Across the Tall Buildings.
Keywords: bushes, cartoon, child, fantasy, female, flowers, girl, green, human, kid, ladder, lady, leaf, leafy, leaves, little, magical, pathway, person, petal, plants, stairs, stalk, stem, steps, way, young.


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I received a sample of Essentia Water in exchange for this review. All opinions are my own.
How are your New Years' Resolutions going? Still holding strong? Hopefully today's post will help with them. It's an easy one to keep actually.
I'm going to let you in on a little secret of mine. I am able to lose more weight when I drink lots of water.
A few months ago I mentioned the importance of maintaining proper hydration levels. This is especially true when you are trying to lose weight.
I've heard a few people say they don't want to drink a lot of water because it will make them gain weight, they will retain the "water weight." Actually, just the opposite is true. When you drink more water, your body flushes the water faster, and it flushes toxins as well.
You should be drinking at least half your body weight in ounces every day. If a person weighed 150 pounds, they should drink 75 ounces of water. That might seem like a lot of water, but it adds up very quickly.
I usually drink about 30 ounces with each meal, and I have about 16 ounces in between each meal. That's more than half my body weight in ounces, but I love drinking water, and drinking more water tricks my stomach into feeling fuller. If I feel a craving coming on, I drink about 16 ounces of water first, then wait a couple of minutes to see if my craving has dissipated. Most of the time it has. When I've had my allotted serving at dinner, but still feel a little hungry, I'll drink a glass of water and that usually makes me feel more full.
I want to make a little clarification here about the type of water to drink. I know a few people that drink a lot of carbonated water. In fact, that's mainly the type of water they drink. Carbonated, or sparkling, water is better than no water, but no carbonation beats carbonation every time. Your body can do funny things with extra carbonation, but that's another topic for another day.
There are a lot of water choices available, which ones are the best? Like I mentioned in my post a few weeks ago, I had my eyes opened when I watched the documentary, Fed Up. I knew before that everything had sugar, but I really started doing the math, and paying closer attention to the food I was eating. The same holds true for the drinks I consume.
I made a quick trip to the grocery store and found over 6 different types of water. Not just 6 different brands of water, there are more like 50 brands of water, but 6 types of water.
Sparkling Ice has carbonation, plus artificial sweeteners for taste. Both ingredients aren't great for your body.
Gatorade or Powerade have either sugar or artificial sweeteners depending on if you get the "Zero" variety. Yes, there are electrolytes in Gatorade or Powerade, but there are healthier ways to get electrolytes. I really only drink these sports drinks while I am playing sports, ie., running a marathon.
Coconut water. I like coconut water, it has electrolytes, it has natural sugars (as long as you check the ingredients and there aren't added sugars), it also has the great vitamins and minerals that coconuts contain. But, you can't drink 100 ounces of coconut water or you would never leave the bathroom. I add a little coconut water to my morning smoothie, and about 1/4 cup to 20 ounces in my water bottle.
Propel water just doesn't taste very good, in my opinion. It has similar issues with Gatorade or Powerade, artificial sweeteners, with a lot of aftertaste.
Vitamin Water. These drinks just make me laugh. There are some vitamins added, yes, but water? Not much. There is a lot of sugar in these drinks. They are tasty, and I drink them when I am pregnant, because I can't drink plain water while pregnant, but the sodium content can be very high, depending on the flavor you buy. Not really a healthy drink, but tasty.
A new water has recently come out on the market, pH balanced water. Have you heard that adding lemon to your water will balance the pH levels in your body? It's true, but not everyone enjoys lemon in their water. Instead, companies are now adding specific minerals and vitamins to provide a higher pH level in the water.
I recently received a sample of Essentia Water to review. The shipment arrived the day we got home from Disneyland. You know you can't eat super healthy while you're at Disneyland, and I always feel a little bloated and/or sluggish after flying and traveling for any specific time. It was so nice to see the box full of Essentia Water when we got home.
I just started a weight loss challenge, and yes, I was trying to be careful about what I ate while we were in Disneyland, but for all the reasons I just mentioned, I gained about 3 pounds while we were gone. I received 2 50 oz bottles, 2 33 oz bottles, and 2 16 oz bottles. I drank one of the 50 oz bottles, and one of the 33 oz bottles within 8 hours of being home. The next day I drank the rest of the Essentia water, plus a little more from my fridge after I ran out. I lost 4 pounds in 3 days. I know some of that can be attributed to a better diet once I got home, and more strenuous exercise, but I know part of that weight loss was from the water. I felt cleaner inside and out after drinking the Essentia Water. I knew my body was getting rid of the excess toxins from the food and traveling. It was nice!
The Essentia Water tastes great too! I was a little nervous there would be an aftertaste with all the added minerals, and pH level of 9.5. There was absolutely no aftertaste. The Essentia Water tastes better than my fridge filtered water. I even offered some to my mother-in-law (they traveled with us), and she loved the taste of the water, she usually has to add some flavoring to her water.
So, here's the summary of all this information. Drink lots of water if you are trying to lose weight, or if you just want to feel healthier. And try some pH balanced water to see if that doesn't help curb your cravings, and improve your mood.
I've got a few more health tips I'll be posting in the next few weeks, so keep checking back!


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Augmented intrarenal ANG II stimulates IL-6, which contributes to renal injury. The expression of intrarenal angiotensinogen (AGT) is enhanced by increased intrarenal ANG II in human renin/human AGT double transgenic mice. ANG II also augments AGT expression in hepatocytes and cardiac myocytes. However, the mechanisms underlying AGT augmentation by ANG II and the contribution of IL-6 to this system are poorly understood. This study was performed in human renal proximal tubular epithelial cells (HRPTECs) to test the hypothesis that IL-6 contributes to the upregulation of AGT expression by ANG II. Human kidney-2 (HK-2) cells, immortalized HRPTECs, were incubated with 10−7 M ANG II and/or 10 ng/ml IL-6 for up to 24 h. AGT mRNA and protein expressions were measured by real-time RT-PCR and ELISA, respectively. The activities of NF-κB and STAT3 were evaluated by Western blotting and EMSA. Stimulation with either ANG II or IL-6 did not significantly alter AGT mRNA or protein expression. In contrast, costimulation with ANG II and IL-6 significantly increased AGT mRNA and protein expressions (1.26 ± 0.10 and 1.16 ± 0.13 over control, respectively). Olmesartan, an ANG II type 1 receptor blocker, and an IL-6 receptor antibody individually inhibited this synergistic effect. NF-κB was also activated by costimulation with ANG II and IL-6. Phosphorylation and activity of STAT3 were increased by stimulation with IL-6 alone and by costimulation. The present study indicates that IL-6 plays an important role in ANG II-mediated augmentation of AGT expression in human renal proximal tubular cells.
- angiotensinogen
- kidney
- renin-angiotensin system
- cytokines
the renin-angiotensin system (RAS) plays important roles in blood pressure control and the regulation of electrolytes and body fluid homeostasis. Previous studies have demonstrated the expression of renin and angiotensinogen (AGT), the precursor of ANG II, in multiple tissues (8, 37). These findings led to the concept that locally expressed RAS is involved in the regulation of individual organs in a tissue-specific manner (8, 28, 30). Nevertheless, since intrarenal ANG II is elevated in many forms of hypertension and blockade of RAS components constitutes potent therapies that control hypertension and attenuate renal damage, the renal RAS is acknowledged as a key target for clinical and biochemical studies (26). In the kidney, AGT is expressed mainly in the proximal tubule cells (13, 38), and the expressions of renal AGT mRNA and protein are enhanced in ANG II-infused rats (18, 34). We recently reported that intrarenal AGT mRNA and protein expression is enhanced by increased intrarenal ANG II production in human renin/human AGT double transgenic mice (19). Moreover, ANG II induces AGT expression in an immortalized rat renal proximal tubular cell line (12). These data provide a firm foundation for the hypothesis that ANG II-induced enhanced AGT production in renal proximal tubular cells contributes to further increases in intrarenal ANG II levels.
In rodent tissues and cells, some mechanisms underlying ANG II-induced AGT augmentation have been reported in hepatocytes (3, 16, 23). ANG II induces rat AGT expression via NF-κB activation (3, 16, 23). An acute-phase response element was identified in the rat AGT promoter region, which includes the NF-κB and CCAAT/enhancer-binding protein binding sites (2, 5). This finding revealed a molecular mechanism underlying the augmentation of rodent AGT by ANG II and some cytokines. On the other hand, little information is available regarding the cis- and trans-acting regulators of AGT expression in human cells (2). Although the regulatory mechanisms of human AGT have been clarified by investigations regarding human AGT polymorphism (9, 14), the existence of an NF-κB-binding site in the promoter region of human AGT has not been reported (10, 15). Therefore, it is not known whether NF-κB activity is involved in the expression of human AGT.
ANG II and cytokines contribute to various forms of renal dysfunction (35), and there is substantial evidence of interaction between these factors. In IL-6 knockout mice, the magnitude of ANG II-induced hypertension is attenuated (21). In the kidneys of ANG II-infused rats, IL-6 levels are elevated with the hypertension (31). Furthermore, the disruption of the IL-6 gene attenuated albuminuria, a marker for renal injury, in ANG II-induced hypertensive mice (24). In hepatocytes, IL-6 stimulated AGT secretion (27) and expression via STAT3 activation (15, 29). These data suggest that IL-6 plays a role in the ANG II-mediated AGT positive feedback system (4). However, direct interaction between AGT and IL-6 in the kidney has not been established. In particular, the mechanism underlying the augmentation of AGT by ANG II and the contribution of IL-6 to this system in human renal proximal tubular epithelial cells have not been demonstrated although it is clear that intrarenal AGT is primarily expressed in proximal tubule cells. This study was performed to test the hypothesis that IL-6 contributes to the upregulation of AGT expression by ANG II and that NF-κB and STAT3 participate in the regulation of AGT expression in human renal proximal tubular epithelial cells.
Cell culture.
Human kidney-2 (HK-2) cells, which are immortalized human renal proximal tubular epithelial cells, were obtained from ATCC. The cells were cultured in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen). They were plated at a density of 2 × 105 cells/well in six-well plates. Before stimulation, the cells were exposed to serum-free medium for 24 h. Thereafter, they were treated with 10−7 M ANG II (Phoenix Pharmaceuticals) and/or several concentrations of IL-6 (Peprotech) for up to 24 h (4, 12, or 24 h) in a medium containing 1% serum. To determine the contribution of ANG II type 1 receptor (AT1R) to the effect of ANG II and IL-6 on AGT expression, HK-2 cells were individually treated with ANG II and IL-6 and with a combination of ANG II and IL-6 in the presence of 1 μM olmesartan (Sankyo), an AT1R blocker, for 24 h. Moreover, the role of the IL-6 receptor (IL-6R) in the effects of ANG II and IL-6 was investigated using 2 μg/ml anti-IL-6R antibody (R&D Systems). To deduce the influence of NF-κB in human AGT expression, cells were treated with 10 μM parthenolide, an NF-κB inhibitor (32), for 24 h. To investigate the influence of STAT3 on human AGT expression, cells were treated with 0.2 μM JSI-124 (Calbiochem), an inhibitor of STAT3 activation (1). The treatment with JSI-124 was begun before 2 h of stimulation with ANG II and/or IL-6 because the inhibitory effect of JSI-124 is observed more slowly than STAT3 activation by IL-6 (39).
Quantitative real-time RT-PCR.
Quantitative real-time RT-PCR (qRT-PCR) was performed to evaluate human AGT mRNA expression using a TaqMan PCR system. For total RNA isolation, treated cells were washed with 3 ml of PBS. After PBS aspiration, total RNA was isolated from the cells using a BIO-ROBOT EZ 1 (Qiagen). Subsequently, qRT-PCR was performed as previously described (20). In brief, 20 ng total RNA from each sample was applied to an Mx3000P System (Stratagene) equipped with a Brilliant Single-Step QRT-PCR Master Mix II Kit (Stratagene). All samples were analyzed in triplicate, and the data were normalized based on the expression level of the human GAPDH mRNA. The information of sequences are as follows: human AGT mRNA: forward primer, 5′-GAG AGA GCC CAC AGA GTC TA-3′; reverse primer, 5′-GCT TTG ATC ATA CAC AGC AA-3′; and probe, 5′/6-FAM/CCA ACA GCT TAA CAA GCC TGA GGT/BHQ1/3′; and human GAPDH mRNA: forward primer, 5′-ATC ATC CCT GCC TCT ACT GG-3′; reverse primer, 5′-CTG CTT CAC CAC CTT CTT GA-3′; and probe, 5′/HEX/ACC TGA CCT GCC GTC TAG AAA AAC CTG/BHQ2/3′.
Human AGT ELISA.
To quantify human AGT protein in the culture medium and in the cell lysate, human AGT ELISA was performed. HK-2 cells were cultured in six-well plates with 1.5 ml of medium/well. After treatment with the various agents, the media were collected into tubes, and the supernatants were collected after centrifugation of the media for 5 min at 5,000 rpm. Cells were harvested with 100 μl lysis buffer (IBL), and the cell lysates were then incubated for 30 min at 4°C. Supernatants were collected after centrifugation for 10 min at 10,000 rpm. Human AGT ELISA was performed as previously described (17). Total protein concentrations in the media and cell lysates were quantified using Bio-Rad Protein Assay (Bio-Rad) and a Micro BCA Protein Assay Kit (Pierce), respectively. Data from human AGT ELISA were normalized based on protein concentration.
Western blot analysis.
To detect the expression levels of AT1R and IL-6R proteins, Western blotting was performed. After treatment for 24 h, cells were washed three times with 3 ml PBS. The cells were harvested with 80 μl lysis buffer containing 1% Triton X-100, 150 mmol/l NaCl, 1 mmol/l EDTA, 1% Nonidet P-40, 1 mmol/l Na3VO4, and 0.25% Protease Inhibitor Cocktail (Sigma). The lysates were sonicated three times for 10 s each and centrifuged at 13,000 rpm at 4°C for 30 min. Total protein concentration of the supernatant was quantified using a Micro BCA Protein Assay Kit (Pierce). Next, 5 μg (for AT1R) or 20 μg (for IL-6R) of total protein was applied to a precast NuPAGE 4–12% gel (Invitrogen). The separated proteins were transferred to a nitrocellulose membrane (Bio-Rad). A rabbit anti-AT1R antibody (1:350, Santa Cruz Biotechnology) and an IRDye-labeled anti-rabbit IgG antibody (1:15,000, Li-Cor) were used for AT1R detection. A goat anti-IL-6R antibody (1:150, R&D Systems) and an IRDye-labeled anti-goat IgG antibody (1:15,000, Li-Cor) were used for the detection of human IL-6R. Mouse anti-β-actin antibody (1:1,000, Abcam) and an IRDye-labeled anti-mouse IgG antibody (1:15,000, Li-Cor) were used for the detection of human β-actin. Detection was performed using an Odyssey System (Li-Cor). Data were normalized based on human β-actin protein expression levels. Phosphorylations of p65, a subunit of NF-κB, and STAT3 were detected using Western blot analysis to elucidate participations of these transcriptional factors in the effects mediated by ANG II and IL-6 on human AGT expression. Cells were treated with ANG II and/or IL-6 for up to 30 min. Mouse anti-phospho-p65 (Ser 536) antibody (1:1,000, Cell Signaling), mouse anti-phospho-STAT3 (Tyr 705) antibody (1:250, BD Transduction Laboratories), and an IRDye-labeled anti-mouse IgG antibody (1:15,000, Li-Cor) were used for the detection of phosphorylated p65 and STAT3. It is known that p65 protein is a subunit of NF-κB, and NF-κB activation involves p65 protein phosphorylation (33). Rabbit anti-p65 antibody (1:1,000, Cell Signaling), rabbit anti-STAT3 antibody (1:250, Thermo), and an IRDye-labeled anti-rabbit IgG antibody (Li-Cor) were used to detect total p65 and STAT3. The levels of phosphorylated p65 and STAT3 were normalized based on total p65 and STAT3, respectively.
Binding activities of NF-κB and STAT3 to DNA were tested using EMSA. After treatment for 15 min, the cells were washed with 3 ml cold PBS and then the cells were harvested. Nuclear protein extraction was performed using an NE-PER kit (Pierce). The protein concentration was determined using a Micro BCA Protein Assay Kit (Pierce). Three micrograms nuclear protein was incubated with NF-κB IRDye-700 (Li-Cor) or STAT3 IRDye-700 (Li-Cor) for 30 min. Reagents included in an EMSA Buffer Kit (Li-Cor) were used for the reaction. The reaction was applied to 6% Novex DNA Retardation Gel (Invitrogen). The detection and quantification of shift bands were performed using an Odyssey System. After detection of the shift bands, target bands were identified using the combination of anti-p65 with p50 antibodies (Cell Signaling) and anti-STAT3 antibody (Thermo).
Statistical analysis.
Data are expressed as means ± SD. The data were analyzed using one-way ANOVA followed by a Bonferroni/Dunn multiple comparison post hoc test. P < 0.05 was considered statistically significant.
Effects of ANG II and IL-6 on human AGT mRNA expression.
Augmentation of human AGT mRNA expression was not observed with any combination at either 4 or 12 h (Fig. 1A). The AGT mRNA expression level was not influenced by the addition of either 10−7 mol/l ANG II or 10 ng/ml IL-6 at each of these two time points (Fig. 1A). However, costimulation with 10−7 mol/l ANG II and 10 ng/ml IL-6 significantly increased the expression level of AGT mRNA (1.26 ± 0.10, ratio to control) at 24 h (Fig. 1A). AGT mRNA expression was not increased by any combination either at 48 h (data not shown). The increased expression of AGT mRNA induced by costimulation reached significance at 10 ng/ml IL-6 (Fig. 1B).
Effects of ANG II and IL-6 on human AGT protein expression.
In addition to AGT mRNA expression, costimulation with ANG II and IL-6 increased the expression of human AGT protein in the media (Fig. 2A) and cell lysates (Fig. 2B) after 24 h. The increments in AGT protein expression induced by the costimulation were smaller than those increments in AGT mRNA expression (in medium: 1.15 ± 0.05, in cell lysate: 1.16 ± 0.13, ratio to control). However, the difference is significant. In both the medium and cell lysate, stimulation with either ANG II or IL-6 individually had no effect on the expression level of AGT protein when tested independently.
Effects of olmesartan and IL-6R antibody on AGT mRNA augmentation by costimulation with ANG II and IL-6.
The augmentation of AGT mRNA induced by costimulation with ANG II and IL-6 was inhibited by 1 μmol/l olmesartan (Fig. 3A). In the absence of the costimulation, olmesartan did not influence the AGT mRNA level. We observed that 2 μg/ml IL-6R antibody neutralized the effect of costimulation with ANG II and IL-6 (Fig. 3B). Individually, neither olmesartan nor the IL-6R antibody had any effect on AGT expression.
Effects of ANG II and IL-6 on AT1R and IL-6R protein expression.
Addition of either ANG II or IL-6 individually did not induce AT1R expression at 24 h. Furthermore, costimulation with ANG II and IL-6 had no effect on AT1R expression either (Fig. 4A). Similarly, there was no change in the expression of IL-6R at 24 h (Fig. 4B).
Effects of ANG II and IL-6 on NF-κB activity.
Phosphorylation of p65 protein was not affected by treatment with either ANG II or IL-6 at any time point. Although phosphorylation of p65 protein was not observed at 0 min (data not shown), it was detected even in the control after 5 min (Fig. 5A). Costimulation with ANG II and IL-6 facilitated p65 phosphorylation at 10 (1.28 ± 0.21, ratio to control) and 15 min (2.21 ± 0.62, ratio to control, Fig. 5A). The increment in p65 protein phosphorylation by the costimulation was not detected at 30 min (Fig. 5A).
Binding activity of NF-κB to DNA was detected using EMSA. Costimulation with ANG II and IL-6 significantly enhanced the binding activity at 15 min (1.67 ± 0.15, ratio to control, Fig. 5B). Binding activity as well as p65 phosphorylation were detected even in the control (Fig. 5B).
Since the binding activity of NF-κB to DNA was detected even in the control, the contribution of NF-κB to human AGT expression was determined using parthenolide, an NF-κB inhibitor. The inhibitor attenuated the basal expression level of AGT (0.34 ± 0.13, ratio to control) at 24 h in the cell line used in this study (Fig. 5C).
Effects of ANG II and IL-6 on STAT3 activity.
ANG II alone did not induce phosphorylation of STAT3 at any time point. STAT3 phosphorylation was increased by stimulation with IL-6 alone at 10, 15, and 30 min (2.22 ± 0.06, 2.75 ± 0.68, and 1.46 ± 0.14, respectively, ratio to control). Costimulation with ANG II and IL-6 also increased the phosphorylation at these time points (1.95 ± 0.26, 2.02 ± 0.63, and 1.32 ± 0.11, respectively, ratio to control, Fig. 6A).
EMSA showed that the binding activity of STAT3 to DNA was significantly induced by IL-6 alone and by the costimulation at 15 min (1.16 ± 0.04 and 1.33 ± 0.04, respectively, ratio to control, Fig. 6B).
JSI-124 inhibited STAT3 phosphorylation (Fig. 6C) and increment in AGT mRNA expression (Fig. 6D) by costimulation with ANG II and IL-6.
The present results demonstrate the effects of combined ANG II and IL-6 on human AGT expression in human renal proximal tubular cells. Several in vivo studies in rodents have demonstrated ANG II-induced augmentation of renal AGT expression (18, 19, 34). Furthermore, in vitro studies have shown ANG II-induced augmentation of AGT expression in rat renal proximal tubular cells (12), hepatocytes (23), and cardiomyocytes (25). Previous studies have also shown that IL-6 stimulates AGT secretion (27) and expression (15, 29, 36) in hepatocytes. In the present study, stimulation with ANG II alone did not increase the expressions of human AGT mRNA or protein in HK-2 cells. Although stimulation of IL-6 alone also failed to increase human AGT expression, costimulation with ANG II and IL-6 increased the expression levels of both AGT mRNA and protein at 24 h. Since it is well known that ANG II increases intrarenal IL-6 levels (31), ANG II and the IL-6 induced by ANG II may collectively increase human AGT expression in vivo. Although AGT production in HK-2 cells is lower than that in HepG2 cells (7), renal proximal tubular cells are the main source of AGT in the kidney (13, 38). Therefore, ANG II and IL-6 can synergistically induce large changes in the amount of AGT in the human kidney even if its induction ratio is small, as shown in this study. Augmentation of AGT expression in the kidney by IL-6 has not been reported; thus this study provides novel information relevant to intrarenal AGT regulation. Moreover, the results indicate that ANG II-dependent induction of AGT expression requires IL-6 as a costimulatory factor in human renal proximal tubular cells.
It has been reported that oxalate and IL-1 stimulate IL-6 production in HK-2 cells, indicating that HK-2 cells can produce IL-6 by in response to various stimuli (6, 11). Therefore, it is possible that ANG II induces endogenous IL-6. The reason that single stimulation with ANG II alone did not induce AGT expression may be the low concentration of induced IL-6. In support of this, costimulation with ANG II and a low concentration of IL-6 did not influence AGT expression (Fig. 1B).
Olmesartan inhibited the effect of costimulation with ANG II and IL-6 on AGT expression, indicating that AT1R is critical for AGT enhancement by ANG II. Moreover, IL-6R antibody also neutralized the effect, suggesting that these agents exert a synergistic effect, even though they do not independently induce human AGT expression. These findings suggest that AT1R blockers may attenuate the secondary pathophysiological effects of ANG II even though ANG II alone does not exert any direct effects, and this may partially account for the powerful renoprotective effects of AT1R blocker beyond its depressor effect.
It has been reported that IL-6 increases AT1R expression in vascular smooth muscle cells (40). Thus we evaluated the possibility that ANG II increases IL-6R expression and/or IL-6 increases AT1R expression. However, treatment with either ANG II or IL-6 alone or with their combination did not increase the expression of AT1R or IL-6R (Fig. 4). Hence, these results indicate that the mechanism underlying human AGT upregulation by costimulation in this cell line is independent of the changes in the expression levels of their receptors.
Treatment of cells with the combination of ANG II and IL-6 facilitated the phosphorylation of p65 protein (Fig. 5A). Furthermore, NF-κB activation in response to costimulation with ANG II and IL-6 was revealed by EMSA (Fig. 5B). ANG II-induced NF-κB activation has been shown in many studies (3, 16, 23), and high basal activity of NF-κB in HK-2 cells has been demonstrated (6). In our study, high basal activity of NF-κB was observed (Fig. 5, A and B, in control), and basal AGT expression level was decreased by NF-κB inhibitor (Fig. 5C). Although an NF-κB-binding site in the human AGT promoter region has not been identified, these results indicate that NF-κB activity obviously relates to AGT expression in the human kidney as well as in the rodent kidney. High basal NF-κB activity may reflect the high production of AGT in proximal tubule cells in the kidney.
Interestingly, STAT3 phosphorylation was increased by IL-6 alone as well as by costimulation with ANG II and IL-6 (Fig. 6A). Furthermore, STAT3 activation by IL-6 alone and by costimulation with ANG II and IL-6 was revealed using EMSA (Fig. 6B). Moreover, JSI-124 inhibited STAT3 phosphorylation (Fig. 6C) and increment in AGT mRNA expression (Fig. 6D) by costimulation with ANG II and IL-6. IL-6 has been shown to stimulate NF-κB activity through the activation of STAT3 in renal proximal tubule cells (22). Therefore, NF-κB activation by costimulation with ANG II and IL-6 may be achieved through weak activation of NF-κB by ANG II, which is subsequently augmented by IL-6-mediated STAT3 activation. The human AGT promoter region contains STAT3-binding sites, and the activation of STAT3 contributes to the augmentation of AGT expression (15). However, independent stimulation with IL-6 had no effect on AGT expression in HK-2 cells. Although high basal activity of NF-κB might relate to this low sensitivity of human AGT expression to IL-6, further studies are required to confirm this possibility.
In summary, we have demonstrated that ANG II and IL-6 synergistically induce human AGT expression through the activation of NF-κB and STAT3 in HK-2 cells, indicating that IL-6 plays an important role in ANG II-mediated augmentation of AGT expression in human renal proximal tubular cells. Elucidation of the mechanisms underlying this augmentation may lead to new strategies to treat hypertension, cardiac diseases, and renal diseases.
This study was supported by National Institutes of Health Grants R01-DK-072408, P20-RR-017659, and R01-HL-026371; the Health Excellence Fund, Louisiana Board of Regents; Sankyo Co., Ltd. (Tokyo, Japan); and the Consortium for Southeastern Hypertension Control Warren Trust Fellowship Award program (R. A. Gonzalez-Villalobos).
The authors acknowledge the excellent technical assistance of Duy V. Tran and My-Linh Rauv (Tulane University).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2008 the American Physiological Society


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King County this week netted more than $500,000 of Nonhighway and Off-Road Vehicle Activities (NOVA) grants.
Trail and campsite maintenance, Forest Service patrols and a new bridge over the Snoqualmie River for hikers, bikers and horseback riders are among seven outdoor-recreation projects in King County that have been awarded federal grants.
The county this week netted more than $500,000 of Nonhighway and Off-Road Vehicle Activities (NOVA) grants to help fund cross-country skiing, mountain biking, fishing, sightseeing and other activities throughout Washington.
Such financial help is vital to the upkeep of hundreds of miles of trails that snake through state forests and are enjoyed by thousands of hikers each weekend, said Doug Schindler, field-program director for the Mountains to Sound Greenway Trust, a nonprofit that works to preserve open spaces along Interstate 90.
"People think that trails just sort of are there," Schindler said. "But trails don't build themselves. Trails don't maintain themselves. It takes a lot of work."
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The projects include:
$100,000 for the state Department of Natural Resources to hire a Washington Conservation Corps crew for two years to maintain 55 miles of trails in the Tiger Mountain and Snoqualmie state forests. The crew will remove downed trees and brush and rebuild trail surfaces and drainage.
$100,000 for the U.S. Forest Service to build a 100-foot bridge across the Snoqualmie River near North Bench and Goldmyer Hot Springs, to provide a safe route for hikers, bikers and equestrians along the Middle Fork Trail system and trails leading into the Alpine Lakes Wilderness.
$62,404 for the U.S. Forest Service to fund four seasonal forest-protection officers, and travel costs for two senior volunteers this summer, in the Mount Baker National Forest. They will patrol the 300,000 acres and 50 trailheads of the Snoqualmie Ranger District, plus thousands of campsites and 24 outhouses.
Snohomish County received more than $200,000. About half of that amount will go to repair the Darrington Trail in the Glacier Peak Wilderness, which sustained $4 million in damage after a flood in 2003 that washed many trail bridges below Glacier Peak downstream. Other Snohomish County projects include maintenance work at various trailheads and campgrounds.
The state Interagency Committee for Outdoor Recreation awarded a total of $5.7 million in grants throughout Washington to help fund 71 projects in 27 counties. Funding comes from 1 percent of federal gas taxes, plus fees levied on off-road-vehicle users and dealers, said Susan Zemek, a committee spokeswoman.
Among Washington counties, Kittitas County received the most federal NOVA grants this year: more than $1 million to maintain and rebuild trails and restore the popular Kachess campground, among other projects.
Karen Gaudette: 206-515-5618 or email@example.com


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Nadesico (GN 3)
Title: Nadesico
Volume: GN 3
Pages: 163
Distributor: Central Park Media
Release date: 2002-04-15
Suggested retail price: $15.95
Set in 2142, Nadesico is a fast-paced, offbeat, science fiction manga. Nadesico is reminiscent of classic anime of the past, combing elements of Macross and Gundam with an Asamiya twist. When Mars is attacked by alien invaders, the United Earth government deploys the large battleship Nadesico into space to fight the alien menace. Manned by a mostly female crew led by the lovely captain Yurika Misumaru, the Nadesico also has the accidental hero, Akito Tenkawa. In this volume, Akito searches Mars for clues to his past, and a mysterious space cruiser from the past appears—the Yamato (shades of Star Blazers)! When the Nadesico's crew is reassigned, Yurika receives a mysterious summons...a call to her as the Shaman Queen of the Yamato Pantheon! Original story and art by fan-favorite Kia Asamiya (Dark Angel, Star Wars Episode One: The Phantom Menace Manga)!
(added on 2011-09-29, modified on 2011-09-29)
- Encyclopedia information about Nadesico (manga)


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Megger MOM2 220A Micro-Ohmmeter
The Megger MOM2 is an innovative hand held Micro-Ohmmeter, designed with both portability and high current output specification in mind.
Able to output up to 220A DC in order to provide a high accuracy resistance measurement as low as one micro-ohm (0.000001 Ohm), the Megger MOM2 is suitable for a wide range of testing applications in almost any location. This is due to an ultra-capacitor that is designed to store and discharge a huge amount of energy due a very low internal resistance, unlike a standard capacitor.
Whilst testing, the ultra-capacitor is discharged across the test object, whilst the voltage drop is simultaneously monitored. Both voltage and current readings are synchronously sampled and averaged, allowing the Megger MOM2 to display an accurate resistance measurement.
The Megger MOM2 also features DualGround testing certification. This means that the micro-ohmmeter features groundbreaking technology and methods that enable for a safe, fast and easy workflow, with both sides of the test subject grounded (earthed) throughout the test process. This means the Megger MOM2 is certified for use by many utilities requiring a safety earth connection in place during sub-station outages.
Supplied with a strong rubber holster within a hard carry case, the portable design of the Megger MOM2 micro-ohmmeter makes it the ideal instrument for use in the field and substations alike. Fully charged, an engineer can expect a full day's worth of testing without further recharge, whilst being able to store and download 190 test values via Bluetooth to a PC or Laptop using the MOMWin software supplied.
Why test resistance at such a low value?
If the contact resistance on a high current electrical joint is too high, temperature rises and power loss are inevitable leading to a host of problems such as overheating joints and electrical equipment failure. The following table demonstrates the importance of low resistance joins within high current applications:
Current Contact Resistance Power Loss 10,000A (10kA) 1 milli-Ohm 100,000 Watts (100kW) 10,000A (10kA) 0.1 milli-Ohm 10,000 Watts (10kW) 1,000A (1kA) 1 milli-Ohm 1,000 Watts (1kW) 1,000A (1kA) 0.1 milli-Ohm 100 Watts
Key Features
- 0-220A DC current output
- Battery powered
- Lightweight – 1kg
We Have a Choice Of Three MOM2 Kits:
Includes 1.3m (4ft) test cable
with Kelvin probes
Includes 3m (10ft) test cable
with Kelvin clamps
Includes 5m (16ft) test cable
with Kelvin clamps
MOM2 with Kelvin Probes
2 Included - -
MOM2 with 3m Kelvin Clamps
1 Included 1 Included -
MOM2 with 5m Kelvin Clamps
1 Included - 1 Included
SpecificationPlease click here for full product specifications
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text: OUT THERE (2010) by Abstract Source published on 2011-01-20T15:35:34Z Tech House - Abstract Source - Out There Genre House Comment by M.J.E (Official) good work...... 2012/08/27 07:11:19 +0000 Comment by Fuller Dj Nice song in a nice work! I like this kind of synths and transitions! Check out my song if u want and follow me if u like (pregressive house) Dear Love by Hispánico. 2012/07/13 17:22:02 +0000 Comment by Mariano Fonrouge niceee track !! check: http://soundcloud.com/marianofonrouge/vieja-loca-mariano-fonrouge 2012/05/01 05:30:34 +0000 Comment by Ivan Hermez hello, I like your track, if your disc has the other sign with a label, send it to me I'll singne in senssual records. here is my email address email@example.com 2012/04/05 18:23:48 +0000 Comment by JAKKO Awsome...PLz if you have time check my page ! 2012/03/23 02:49:42 +0000 Comment by recentmatters http://soundcloud.com/vee-and-c/vee-v-c-live-shortcut 2012/02/29 18:39:36 +0000 Comment by TommyBielecki where can i download this?!!? 2012/02/02 23:22:28 +0000 Comment by DINOMYT This is by far one of if not the greatest most inspirational track i have heard in a loooooong time, years!! absolutely wonderful. top notch from start to finish. 2012/02/01 16:14:39 +0000 Comment by Zego12 Primo House! 2011/12/20 07:01:07 +0000 Comment by Y3NO (TreeOfHouse) WOOOooooWWW great track, listen my last groovy tech house sound @ http://soundcloud.com/dj-yeno/dj-yeno-feel-the-groove 2011/12/11 21:16:23 +0000 Comment by Spectrum1 Great work! I Would Appreciate your Feedback on my mixes cheers:) 2011/12/10 00:13:16 +0000 Comment by samuse bounce baby 2011/11/21 15:16:40 +0000 Comment by MIKE-K (OFFICIAL) Good work on this mate.. Please be so kind to check out my first ever set... Comments would be very much appreciated.. Thanks MIKE-K 2011/11/05 19:37:42 +0000 Comment by yumenova @hyper-kube-1: This part is so cool !! I wanna listen dis in the club. 2011/10/25 19:54:53 +0000 Comment by S7any Nicee :) 2011/10/06 20:43:16 +0000 Comment by sickdub (real) this is super tight m8, really like this synth pad or whatever, just building...nice 2011/10/02 06:25:39 +0000 Comment by KfB Great track blogged at WWW.BEATSNOTJUNK.BLOGSPOT.COM 2011/09/05 12:37:05 +0000 Comment by Öteli & Flöteli wahnsinn... 2011/08/31 16:09:42 +0000 Comment by Stereo Statik Records deep! Good! www.stereostatik.com 2011/08/31 05:27:26 +0000 Comment by Luca "1House" Epifano ♫ ♫ love dis! 2011/08/25 18:54:28 +0000 Comment by JEAN AGORYA good 2011/08/20 18:04:09 +0000 Comment by Hyper-kube Always loved your use of the B4 plugin, makes your tracks so distinctive! 2011/08/14 15:06:49 +0000 Comment by Hyper-kube Great work m8! 2011/08/14 15:04:25 +0000 Comment by Abstract Source @youhighbro: Yeah you could say that! how do i submit tracks? 2011/08/13 20:17:46 +0000 Comment by MarcelloImmbrizi Very Crazy! 2011/08/13 17:23:26 +0000 Comment by XFRD This track is amazing man ! 2011/07/15 13:57:28 +0000 Comment by youhighbro an odd chance to run into u on here. i'm hunting for new underground tunes. I totally like the amount of awesome music I've found on soundcloud. Check out my site site http://youhighbro.com/ if your a stoner :D and if you want to submit some music I post random stuff I like to http://koolbro.tumblr.com/ thanks- You High Bro? 2011/07/13 10:19:29 +0000 Comment by SMOOTRAB nice & full sound 2011/07/06 23:23:11 +0000 Comment by chrisby like this sound... groovy-moovy... ;) 2011/07/01 18:26:23 +0000 Comment by Triickz Nice buildup much?? I THINK SO. 2011/06/18 15:04:52 +0000


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I am currently in my first year at the New Zealand Broadcasting School studying a Bachelor of Broadcast Communications - Broadcast Journalism. The course is fantastic and you can't get it anywhere else, I absolutely love it.
The relationship the industry has with the school and the tutors is second to none, which gives us as students the best possible springboard into the professional world. I plan to travel overseas and I hope with the industry I'm going into I can do that. The industry is incredibly exciting and with the help and guidance of CPIT I can follow my ambitions and goals.
Upon successful completion of my degree I intend to commence working in television with the ultimate aim of becoming a broadcasting journalist in one of New Zealand's leading news media companies.


text:
Languages: English
Seller: Collins Publishing
The CEN® (Certified Emergency Nurse) certification is the certification specific to emergency nursing and measures the attainment of a defined body of nursing knowledge pertinent to that particular specialty. A role delineation study is conducted approximately every five years to assure the examination reflects current emergency nursing practice. Although there are CENs throughout the world, the CEN exam is based on emergency nursing practice in the United States.
Comprehensive 500 questions for Emergency Nursing. Updated for 2013. Questions include instruction according to the BCEN: Board of Certification for Emergency Nursing on :
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text:
When most people think about buying life insurance, they don't realize how many different options there are to choose from. Choosing the best policy requires understanding each option, and then knowing if that options is right for you and your family. Types of life insurance that a person or family might see while shopping will include term life insurance, whole life insurance, and simplified issue, among others.
The main differences in the policies concern the amount of money paid for the benefit, the length of time the insured is covered, and the application process required to gain approval of the policy. Depending on a person's circumstances, one type of policy might be more beneficial than another. The first step to choosing a policy requires understanding each type's differences.


text:
| While designers are embedded in a clinical environment,
embedding a clinician on-staff with a design team
also improves the availability of feedback.
Overcoming regulatory pitfalls of medical device development with early clinical involvement.
Overcoming regulatory pitfalls of medical device development with early clinical involvement.
For medical product development, the rigors of input gathering and iterative concept testing may slow down innovation process and subsequently increase costs. Building on user-centered design principles, creating an Embedded Design Team with clinicians and design professionals in a co-creation atmosphere provides a revolutionary way to drive speed to market.
For companies today, meeting regulatory guidance of the U.S. Food and Drug Administration (FDA) and international standard organizations is about improving patient safety by applying principles of user-centered design and demonstrating them adequately throughout the process. Regulatory guidance requires design input from clinicians at frequent touch points to mitigate the risks to patient safety. But, every clinician touch point has costs—in terms of both money and time.
In the past, historical design approaches included voice of the consumer input early in the process and then later at validation. Creating an Embedded Clinician/Design Team by embedding designers in a clinical environment or embedding clinicians on a design team takes user-centered methodologies to the next level. Here are five ways to enhance medical product development with an Embedded Team:
1. Start with the Users
By embedding designers in the medical environment for observation, empathy is greatly enhanced. Kaleidoscope's Chief Medical Officer and practicing Pediatric Surgeon Sean Barnett, MD, MS shares the importance of understanding the physician's ecosystem and needs. "When you are in the consumer goods space, most people have purchased or used a
consumer good like soap or laundry detergent," says Barnett. "However, the majority have never performed or even seen a sleeve gastrectomy for example. Embedding designers helps build their understanding. And the earlier in the product development cycle the better."
Embedding works both ways. While designers are embedded in a clinical environment, embedding a clinician on-staff with a design team also improves the availability of feedback. "Clinicians get excited to work on new products as there is never a shortage of problems to solve. Often the biggest hurdle is creating an interactive environment between the end-user and the designer. With this model, the biomedical engineers can walk down the hall and get clinical feedback immediately," says Barnett. "That's golden."
2. Build Things Early
Whether using an Embedded Clinician/Design Model or even an historical model, it helps to build things as quickly as possible. "After deep upfront research, it is important to move quickly
to concept development," says Chris Hammond, Director of Insights and Innovation at Kaleidoscope. "Create artifacts for users to respond to. Put things in clinicians' hands. We are asking people about the future, something they have not seen yet. You've got to show it to them."
From a regulatory standpoint, it is important to document and track these touch points and changes in design requirements. "Regulatory pathways take care of themselves. Plus, we have a better understanding of tasks and how best to achieve goals," says Hammond.
3. Iterate Fast
Set short cycles and don't set expectations. Step back and ask questions with users frequently to help mitigate risk. For example, Kaleidoscope wanted to understand the intuitiveness of a medical device with a user-interface screen for a global medical technology company.
"It's not always feasible to embed a clinician, so we created an interactive, collaborative five-day process," says Hammond.
"In the first few days, the team worked deeply with a series of nurses and doctors to create, test and refine an interactive prototype," says Hammond. "The working sessions were much more effective because of the early and deep clinical involvement. Issues like the need for a "home icon" on all screens and the problems of using the same interface for both home and clinical use were discovered and resolved. Our user-centered validation held on the fifth day went smoothly. Spending more money upfront made costs much more predictable downstream."
4. Design for the Ecosystem
Designing medical products is about much more than a single physician using a single device. An embedded clinician can help represent the needs of all stakeholders including patients, doctors, nurses, technicians and supply professionals. In another Embedded Design Team example, Kaleidoscope partnered with a regional children's hospital. "We set up a broad needs-based approach for the under-served pediatric market and embedded Kaleidoscope designers and engineers within the hospital," says Barnett. Meshing design professionals and clinicians created a nimble team that was able to discuss problems, talk the same language and come up with solutions quickly.
5. Create Environments for Collaboration
"We have been in business for almost 25 years, working with big Fortune 100 companies and understand what it takes to bring a product to market," says Hammond. "We saw that clinical access was a challenge for companies. For example, Company-XYZ shows up with a contract but the surgeon doesn't want to sign it because they are worried about their intellectual property and their time. They are busy taking care of patients. What is unique about Embedded Teams is that clinicians help drive the process and can see the positive impact on the industry that they are making as a whole."
Embedded Teams add agility to product development but don't replace the need for formal formative user testing with a broad sampling of users. "There may be bias," says Hammond. "Embedded Teams make formal testing more effective and many issues are resolved beforehand."
About Kaleidoscope Innovation and Product Design
Headquartered in Cincinnati, Kaleidoscope is a global innovation and product-design firm with five locations and more than 80 employees. We transform business through Purpose Driven Innovation™ by leveraging our expertise at the intersection of consumer insights, technology and health and wellness.Learn more about our medical product development capabilities.
A board certified adult and pediatric general surgeon, engineer, anatomist and scientist, Dr. Sean Barnett brings a multimodal approach to medical-device design and thinking. He completed his general surgery training and fellowship in surgical infectious disease at the University of Minnesota as well as a fellowship in general and thoracic pediatric surgery at Cincinnati Children's Hospital Medical Center. During his tenure at Cincinnati Children's Hospital, he served on staff as a general, core trauma and bariatric surgeon as well as an Assistant Professor of Surgery through the University Of Cincinnati College Of Medicine. Additionally, Dr. Barnett presided over the Operating Room Equipment and Standards Committee and the Innovation Consortium where he collaborated with numerous companies including Kaleidoscope. As Chief Medical Officer at Kaleidoscope, Dr. Barnett provides a wealth of clinical and corporate medical knowledge and leadership. He remains clinically active and is an Associate Professor of Surgery at the Boonshoft School of Medicine.
Chris Hammond is passionate about the opportunity space between research and design. He grew up in a family of engineers but quickly developed a love of creating and sketching. After ten years of working in the corporate sphere, he joined the team as a Senior Designer. Now he now heads up Kaleidoscope's insight and innovation team, and aims to lead with courage, integrity, and empathy.
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