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To Purchase # 14672S
|100 µl (10 western blots)
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PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
IMP2 (D4R2F) Rabbit mAb #14672
Western blot analysis of extracts from Hep G2, SK-MEL-5, and A-204 cells using IMP2 (D4R2F) Rabbit mAb.Learn more about how we get our images
Gallery: IMP2 (D4R2F) Rabbit mAb #14672
Western Blotting Protocol
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
- 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
- 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
- 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
- Nonfat Dry Milk: (#9999).
- Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Wash Buffer: (#9997) 1X TBST.
- Primary Antibody Dilution Buffer: 1X TBST with 5% nonfat dry milk; for 20 ml, add 1.0 g nonfat dry milk to 20 ml 1X TBST and mix well.
- Biotinylated Protein Ladder Detection Pack: (#7727).
- Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
- Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
- Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody (#7074).
- Detection Reagent: SignalFire™ ECL Reagent (#6883).
B. Protein Blotting
A general protocol for sample preparation.
- Treat cells by adding fresh media containing regulator for desired time.
- Aspirate media from cultures; wash cells with 1X PBS; aspirate.
- Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
- Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
- Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
- Microcentrifuge for 5 min.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
- Electrotransfer to nitrocellulose membrane (#12369).
C. Membrane Blocking and Antibody Incubations
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
I. Membrane Blocking
- (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
- Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
II. Primary Antibody Incubation
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product datasheet) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of TBST.
- Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and Anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
- Proceed with detection (Section D).
D. Detection of Proteins
Directions for Use:
- Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
- Prepare 1X SignalFire™ ECL Reagent (#6883)by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
- Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Western Blot Reprobing Protocol
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Wash Buffer: Tris Buffered Saline with Tween® 20 (TBST-10X) (#9997)
- Stripping Buffer: To prepare 100 ml, mix 6.25 ml of 1M Tris-HCl pH 6.8, 10 ml of 20% SDS and 700 μl β-mercaptoethanol. Bring to 100 ml with deionized H20. Make buffer fresh just prior to use.
B. Protocol
- After film exposure, wash membrane four times for 5 min each in TBST. Best results are obtained if the membrane is not allowed to dry.
- Incubate membrane for 30 min at 50°C in stripping buffer (with slight agitation).
- Wash membrane six times for 5 min each in TBST.
- (Optional) To assure that the original signal is removed, wash membrane twice for 5 min each with 10 ml of TBST. Incubate membrane with LumiGLO® with gentle agitation for 1 min at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.
- Wash membrane again four times for 5 min each in TBST.
- The membrane is now ready to reuse. Start detection at the "Membrane Blocking and Antibody Incubations" step in the Western Immunoblotting Protocol.
posted June 2005
revised June 2016
|Western Blotting
IMP2 (D4R2F) Rabbit mAb recognizes endogenous levels of total IMP2 protein. This antibody recognizes both the 66 kDa isoform 1 and the 62 kDa isoform 2. This antibody may cross-react with an unidentified protein of 40 kDa.Species Reactivity: Human, Mouse, Monkey Species predicted to react based on 100% sequence homology: Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IMP2 protein.
Insulin-like growth factor-II mRNA-binding proteins (IMPs) belong to a family of zipcode-binding proteins (1,2). Three members of this family, IMP1, IMP2, and IMP3, have been identified (1,2). They contain two RNA recognition motifs, four K homology domains, and were found to function in mRNA localization, turnover, and translation control (1,2). Research studies have implicated these proteins in a variety of physiological and pathological processes, such as growth and development (3), testicular neoplasia (4), and melanocytic neoplasia (5).
A well-characterized polymorphism in the corresponding IGF2BP2 gene is associated with susceptibility to type 2 diabetes in specific ethnic populations (6).
1. Nielsen, F.C. et al. (2002) J Cell Sci 115, 2087-97.
2. Nielsen, J. et al. (2003) Biochem J 376, 383-91.
3. Hansen, T.V. et al. (2004) Mol Cell Biol 24, 4448-64.
4. Hammer, N.A. et al. (2005) Reproduction 130, 203-12.
5. Pryor, J.G. et al. (2008) Mod Pathol 21, 431-7.
6. Zhao, Y. et al. (2012) DNA Cell Biol 31, 713-20.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.


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Reuters Staff
1 Min Read
Oct 13 (Reuters) - Intuit Inc :
* Intuit - Co,american express enter partnership to give quickbooks online small business customers access to short-term financing from american express Source text for Eikon: Further company coverage:
All quotes delayed a minimum of 15 minutes. See here for a complete list of exchanges and delays.
© 2017 Reuters. All Rights Reserved.

subset: commoncrawl


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Tone, Mix, Decay, and Time controls
Luscious, studio-grade algorithms
Trails switch lets reverb decay or cut off on bypass
Models include:
'63 Spring
Reviewed by 4 customers
Comments about Line 6 ToneCore Verbzilla Guitar Effects Pedal:
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PSNI seize cannabis and rescue nine dogs, three ferrets and a goat from Belfast house
Police have uncovered a cannabis factory and rescued animals in ill health at a house on the Old Holywood Road on the outskirts of Belfast.
Cannabis plants with an estimated value of around £15,000 were seized during a planned search by the District Support Team
The PSNI also rescued nine dogs, three ferrets and goat. Police say the animals were all in ill health.
Constable Gemma Gregg said: "During the search we discovered a number of animals who appeared to be in a poor state of health. A local vet was contacted and the animals, including nine ogs, were assessed and transferred into the care of a local animal sanctuary.
"We are delighted to have been able to shut down this cannabis factory and prevent these drugs from being sold in our communities. We are also very pleased to have helped the animals and to ensure they now receive the care they need."
Belfast Telegraph Digital


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I have twelve years of math teaching experience to students in grades 7-12. My areas of expertise include pre-algebra, algebra, and geometry. I love to use technology and hands-on manipulatives to make mathematics more concrete.
While I haven't taught students in grades lower than the seventh grade, I have taken numerous courses and read books on how elementary students think about math. I have also tutored my nieces and nephews that are in elementary school. I'd be happy to meet with... ... More
I studied ASL (American Sign Language) at Fox Valley Technical College in Appleton, WI for a couple years. I took Intro to Deaf, ASl 1, and Communication with the Deaf. I attend a Deaf Congregation in De Pere, Wi weekly to sharpen my skills. I volunteer most of my time meeting with the deaf and hard of hearing, and assisting in bible educational work. ... More
To be an effective teacher, I feel it is important to have a solid understanding about the students you are teaching. I feel it is my job as a teacher to take care of these children and prepare them for their future in our community by being aware of who they are as individuals, using a range of techniques to teach them, understand and be knowledgeable about the content being taught, and utilize the resources at my disposal to give them the best education possible. This involves holding stude... ... More
I have spent 6 years living and working in China, including 1.5 years as a full time student in an intensive language program. My studies have focused not only on survival Chinese as some programs do, but rather on the long term goals of reading, writing, speaking, and listening proficiency. In addition to formal, in-classroom learning and examinations I have also had plenty of real-life tasks to master as I am married to a Chinese national whose family members only communicate with me in Chinese. ... More
I taught first grade for 13 years and tutored elementary students during the summer. The most rewarding part of teaching is watching students reach moments when they discover and master something they didn't know before. I believe all children can learn when someone believes in them and understands that there are many paths to learning and different methods to get there. Children deserve patience, respect, and the best of ourselves.... ... More
I graduated with a BS in Elementary Ed in 1974. I have been going to workshops, conferences, and seminars over the years. I have taken 3 or 4 extra courses to keep up my knowledge and up to date. ... More


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Nowadays, in this timeless medium artists continue to work with wood to create their pieces which reflect their ancient tradition of wood carving. Even though their approaches vary greatly, still there are some work that remains one commonality in their works of wood art. It seems that they each can transform the rigid form into something that is unexpectedly beautiful.
Damiano Taurino whose carving and treatment to the wood was smooth that it may look really like a piece of cloth. And in Earl Martz's sculpture of an owl, its face emerges from the distressed bark of a twisted branch. Others though, still have to celebrate their jagged form and incorporate it directly into their work.
In Cairo, where there is the Egyptian Museum, we can find a statue of a man which was produced from sycamore, possibly came from 4,000 BC. This piece is quite likely to be one of the first in a long tradition of wood carving art.
It's so true that woodworking has a long and rich place in our history. The proof is that early humans used it, along with stone, clay, and animal parts, as some of the first materials they mastered. It still remains relevant today, since many objects in our everyday life are crafted from this sturdy form.


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3522 Beauchamp Street, Houston, Texas
$665,0004 Beds4+ BathsActive
$665,0004 Beds4+ BathsActive
3522 Beauchamp Street, Houston, Texas 77009 - Beautiful open floor plan-3 story, 3 to 4 bdrm or study with 4 ensuite baths and a half bath, located in the highly desired Woodland Heights. Spacious second floor--living/dining/kitchen area with butler's pantry, stainless steel appliances, built-in hutch with wine rack, wine chiller, and cozy fireplace. Third-floor master with double sinks, frameless shower w/double shower heads and clawfoot bathtub. Private backyard has summer kitchen. Large two car garage. Zoned to the Travis Elementary!
Listing Courtesy of Boulevard Realty


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Sponsored by Mill Valley Library Foundation & The Friends
Directed by Ken Burns, Sarah Burns, David McMahon
In 1989, five youths of color from Harlem were detained for unlawful assembly in Central Park. Nearby in the park that night, a white woman jogger was brutally assaulted, raped and left for dead. Detectives hell-bent on making a quick conviction subjected the naïve, hapless youths to hours of escalating interrogation. Tired, poor and hungry, they were easily manipulated into confessing to what would infamously become known as the "wilding." All five served six to 13 years in prison for a crime they didn't commit. Master documentarian Ken Burns, continuing his exploration of race and power in America, retraces the missteps of the power elite, the media, the lawyers and police in a city split along race and class lines. In unraveling this miscarriage of justice, the film does much more to restore the humanity of these five wronged individuals than did the confession by the actual assailant in 2002.
Film Note Writer: Carol Harada
Sign up for MVFF Newsletter © Copyright 2013. California Film Institute and Mill Valley Film Festival. All Rights Reserved. | Login | Website:
studio.23 The Mill Valley Film Festival is produced by the California Film Institute, a 501(c)(3) not-for-profit. Celebrating and promoting film as art and education.


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Results 1 to 3 of 3
03-28-12, 12:49 #1Registered User
- Join Date
- Mar 2012
- Posts
- 2
Database design question for newbie
Hi All, I'm trying to design a database that will be implemented as a SQL db back-end for a Java GUI app. The project is for a theater ticket sales s/w product. Part of the project requires an image of the seating layout for a venue that the user can click on to select seats for sale. This information has to be stored in a database for retrieval at later times. The database will also be able to store information for multiple performance at different dates/times, each with their own map of seats for sale.
Here is my concept for two tables to store this information:
Table1: Performance Event Instance
Date date
Time INT
GroupID TINYINT
Venue VARCHAR(20)
Title VARCHAR(40)
Map ??
Table 2: Map
Seat# VARCHAR(2)
Seat Area VARCHAR(10)
Status INT
X-coord FLOAT
Y-coord FLOAT
My question is how do I tie the map with multiple columns to a row in the performance table? I don't see how I could do a table join with the UID from performance because one table describes one seat layout map while one table will describe many performance events. It's almost like I'd have to do a nested table of some sort.
I'm investigating possibly using a BLOB where I serialize a Java object and store it in the database and just contain all of the information in the BLOB. This would work nicely with my Performance Event Table because each BLOB would fit directly into the Map cell for each row. However, I don't have any experience with serialization so thought their might be an easier solution.
Thanks for any help you can give to a database newb!
03-28-12, 15:42 #2Registered User
- Join Date
- Feb 2012
- Posts
- 76
If you add a foreign key to the Map table which references the primary key of the Performance table, you can use that as part of the primary key of Map.
If you pack your data into a BLOB, you'll be unable to query over that data, the whole thing will have to be loaded and deserialized each time you want to check it, and you'll have to iterate over the whole table in many cases. If you mean to throw away the good parts of the dbms like data checking and constraints, joins and ad-hoc querying, query optimization, and more, why even use a database?
03-28-12, 18:33 #3Registered User
- Join Date
- Mar 2012
- Posts
- 2
ahhh Foreign Key. After researching the foreign key concept that makes alot of sense, thanks! I was sure their was something like that but I wasn't sure what it was called or how to implement it. Told you I was a database newb.


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Black Moshannon Road Race. Fraternity Row Criterium. Pennsylvania State University. These are the legendary landmarks of the Eastern Collegiate Cycling Conference, and the popular races that draw riders from all over to test their mettle in the Nittany Cycling Classic hosted by Penn State. With finals starting for some schools and Easter Sunday, there was a noticeable drop in rider participation- nevertheless, teams still made the trek to hunt for more points for the season.
Saturday opened up to wispy blue skies and temperatures rising into short-sleeve weather. Race headquarters and trainers for set up for the day for a team time trial and road race at Black Moshannon State Park in the rolling heart of Pennsylvania. For the first morning race, there was a 9.5 mile team time trial with power climbs and descents that promised fast times for seasoned riders. Interestingly enough, some of the more veteran teams were absent from the races. The hosting Penn State did not submit a men's A team, and Northeastern ended up seven miles off course. The winners of the A fields were incredibly unique- Massachusetts Institute of Technology (MIT) set the bar with a time of 22:48.50, with the regulated four-man team size comprised of two men's A riders, a men's B and a women's A racer. On the women's side, Columbia University was the sole entrant and default winner of the morning race.
The afternoon road race was the real fixture of the day, if not the entire weekend. A 21-mile loop of 50mph descents and three major climbs, the last of which is a grinding five miler that overlooks the valley. In the men's A race, it was a constant game of hard solo attacks, the pack reeling in riders off the front and dropping even more off the back. Nearly half the field dropped out or was pulled from the three-lap struggle of a race. The final major surge was by Brett Wachtendorf (Penn State), who made his move at the base of the final climb and managed to stay away to win his home race. Wachtendorf passed Zachary Ulissi (MIT), Daniel Holmdahl (Dartmouth College) and Alan Royek (Shippensburg University), a group that unsuccessfully tried to pull away beforehand. The three however would stay away from the rest of the diminished field to hold on for the finish. With Ulissi and Holmdahl- respectively one and two in the men's overall standings- so focused on watching the other's moves, it was an easy thing for Royek to collect himself and put out an unstoppable sprint to take second for the day. The final podium spot was taken by Holmdahl, as he managed to nip Ulissi at the line at the end of a very long race.
In the women's A race, there was a bit more of wait involved before the solo acts began. Making her move up the first time up the major climb was Cecilia Davis-Hayes (Columbia)- whose wheel decided to flat at that moment. The move however encouraged the rest, and emerging over the top was Monica Volk (Penn State Lehigh Valley), Rose Long (Icahn School of Medicine) and Shaena Berlin (MIT). The three would work together to for most of the second of two laps. At the final climb however, Volk would peel off first Berlin and then Long to take the victory: no small feat for a freshman rider in her first collegiate season. For Long and Berlin, it was 'just' a matter of coming across the line to take second and third respectively in the conference's toughest road race of the year. In an impressive display of fitness, Davis-Hayes managed to get another wheel from a support vehicle and rode hard to bridge back up to the pack to finish fifth for the day.
Teams setting up early Easter Sunday at Penn State's Frat Row Criterium were greeted by cheery church goers and hung-over university students. The race was a flat six corner screamer of a race, changing from a narrow chicane to wide pavement turns for a great drag finishing stretch. In familiar fashion, Cecilia Davis-Hayes attacked on the first sprint lap and never looked back. Davis-Hayes would eventually lap and rejoin the main field- a move that all but locked up the win for the day and the lion's share of sprint points to keep the green jersey. The pack however was not content to just sit idle, and a three lady chase group went off the field front: Shaena Berlin, Monica Volk and Elspeth Huyett (Kutztown University). The chase group roared around the course, but was unable to lap the field to catch up to Davis-Hayes. The three racers however managed to stay away from the rest of the field to take the rest of the sprint points. In the finishing sprint, it was Huyett that managed to outgun and outstrip to take victory in the pack sprint. Volk would managed to edge out Berlin for the final podium spot; Berlin however, had the last laugh, as she picked enough points to maintain the overall leader's jersey over Davis-Hayes.
Penn State- lacking a rider in the women's A/B race- was determined to show their worth as a fast cycling and hosting school on their home turf. From the gun their riders attacked and set up high speeds, stripping a full third of the field in the first five laps. In similar fashion to yesterday's road race, no rider successfully managed to get away- even Brett Wachtendorf, the home road race winner, strung out the field towards the end but could not get away. Wachtendorf's real plan however was to turn up the heat and draw attention away from his teammate Wes Kline for a field sprint win. University of Pittsburgh racer Michael Oltman however saw through the ruse and marked Wachtendorf's wheel throughout the final laps of the race. Oltman's hunch paid off, and he came into the finish with a head of steam Kline could not match to take the duel and the day. Kline- who flatted, crashed and rejoined the race on a free mechanical lap- did Penn State proud to take second, with Jules Goguely (Rhode Island School of Design) taking third. Daniel Holmdahl (Dartmouth College) picked up an impressive 20 sprint points to further his hold on the sprinter's jersey- and a final position in the finishing sprint to finally overtake Zachary Ulissi for first in the overall men's standing
The weekend omnium was won by MIT with 210 points. Hosting team Penn Sate finished second (197 points) by a scant point over Columbia University (196 points). With only one more weekend left in the books before the season's and double points on the line for the championship weekend, riders everywhere look with baited breath to see who will take it all- the season leader's jerseys for sprints and overall, the divisional victories for their school and the championship races.
Full results are available from the ECCC calendar.
*Reporting for this recap was made possible by a very detailed e-mail and generous help from MIT's powerhouses Shaena Berlin and Zachary Ulissi. Still, ECCC writer Benjamin Kramer is prone to slip-ups and lacking enough pictures (there can never be enough). Feel free to e-mail him to help make past and future race reports accurate and awesome.*


text:
ago23 days ago
Telephone Engineer, Ex BT, Eastbourne. 01323 44140001323 4414...(click to reveal full phone number). With 29 years of experience in the telecommunications industry, I provide telecom services to customers in Eastbourne and the surrounding area. Additional telephone extension sockets / points fitted around the home for the internet and Sky. I carry out fault finding on noisy and crackly telephone lines, rewiring and relocation of telephone sockets, installation of indoor and outdoor bells, networking of computers, testing and trouble shooting of problems on Broadband, Internet and WIFI. Covering Eastbourne, Seaford, Hailsham, Ploegate, Pevensey Bay and the surrounding area. Eastbourne 01323 44140001323 4414...(click to reveal full phone number).
ago1 day ago


text:
Anopheles sinensis
From Pestinfo-Wiki
|Literature database
|145 articles sorted by:
|• year (recent ones first)
|• research topics
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|• list of natural enemies
Anopheles sinensis Wiedemann, 1828
The mosquito is found in China and neighbouring countries like Taiwan, Indonesia, South Korea, Thailand and Japan. It is zoophilic, preferring to feed on cattle and other domestic animals. However, it also bites humans readily, especially when animal hosts are not available. It is a vector of malaria (Plasmodium vivax).
A. sinensis breeds in various water accumulations but mainly in rice fields. Populations can increase rapidly as the development of the immature stages is completed in only 2 weeks. A. sinensis belongs to the Anopheles hyrcanus group.


text:
This establishment has closed. This page remains for historical records.
Pacific Beach Bar & Grill
- 860 Garnet Avenue, San Diego, CA 92109 | Directions
- 858-272-7278 or 858-272-1242
Additional Info
|11 a.m. to 2 a.m.
|11 a.m. to 2 a.m.
|11 a.m. to 2 a.m.
|11 a.m. to 2 a.m.
|11 a.m. to 2 a.m.
|11 a.m. to 2 a.m.
|11 a.m. to 2 a.m.
Related Links
- "Be A Jerk At Pacific Beach Bar & Grill" · Aug. 22, 2012
JoelSuzuki July 19, 2009 @ 6:39 p.m.
PB Bar & Grille also offers daily food specials. Taco Tuesday is their busiest day other than weekends. The Wednesday specials ($5 pizzas, $5 burger & beer) are also great values.
abbeyrd Oct. 31, 2009 @ 6:44 a.m.
Although it has been sometime since I have eaten a sit down meal here, the food had always been very tasty and the service excellent.
We don't live in the area so only go on an occasional Friday night. Happy hour is until 10pm. We sit at the bar and the menu includes $2.50 wells and domestic drafts plus 50 cent appetizers for each drink you buy.
The appetizers are a small portion of a regular order. All the appetizers are great (garlic fries, philadelphia roll, fried mice which are jalepeno poppers, etc). They really fill you up for cheap. And the service is always great.


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reagents for scientific research laboratory use, namely, immunohistochemistry (IHC) reagents
Business in Tokyo, JP


text:
Blog redirect
May 21, 2008 by Dave Earley
Filed under Uncategorized
Comments Off on Blog redirect
I've ported the blog portion of my site to be the main repository of all things earley edition, so instead of earleyedition.com/blog, the blog now shows on the front page of the site.
Things looked to have worked fine initially, but now I seem to have lost all style and formatting associated with the site.
So apologies for the bare bones look, I'm not quite sure what happened.
From now on, earleyedition.com/blog is redirecting to earleyedition.com, since there is no real difference between the 'blog' and the rest of the content of this site.
I hope you continue to come back, and that it starts looking like normal again some time soon!
It pays to be with iStockphoto
May 9, 2008 by Dave Earley
Filed under Online, Pictures
I've just logged into my iStockphoto account for the first time in over six months, and am mildly surprised to see I have earned some money.
I only have three images available for purchase in my portfolio there. Admittedly they've been there for quite a long time but I wonder if the hit rate is because of good keywords, because there are a large number of purchases comparative to views on one of the photos.
One of the images has been purchased by one out of every five people who have viewed it in the last 18 months, while the other has only seen 1% of viewers purchasing.
In the case of the second image, the higher number of views could be a result of more keywords associated with the image, and therefore less specialised searching allowing people with too many interests to view the image. In the first example, a specific few keywords means only people who want that kind of image are seeing it.
Anyway, I thought it interesting that so few photos added to iStockphoto are still giving some sort of a 'return', no matter how small.
iPhone Australia released on Vodafone
May 6, 2008 by Dave Earley
Filed under Media, Mobile, News, Technology
How's this for awesome? I am of course buying into the hype that is everything Apple, particularly the iPhone, which is yet to be released in Australia.
Vodafone announced today they have signed a deal to sell the iPhone in ten of its global markets, including Australia, 'later this year'.
Tuesday 6 May 2008
Vodafone to offer Apple's iPhone in ten markets
Vodafone today announced it has signed an agreement with Apple to sell the iPhone in ten of its markets around the globe. Later this year, Vodafone customers in Australia, the Czech Republic, Egypt, Greece, Italy, India, Portugal, New Zealand, South Africa and Turkey will be able to purchase the iPhone for use on the Vodafone network.
You have plenty of other multi-purpose phones – smart phone, PDA phone, Pocket PC phone – all of which do lots of good things. Is the iPhone the best? How does it rate against the others?
The mobile world is advancing towards that mythical 'all-in-one' device that can not only effectively meet the demand for multimedia use of phone, video, audio, image and web, but also realistically meet the needs of those publishing content on the go.
It's a mobile revolution. The Nokia N95 can't be bad if it's the mobile platform of choice for the Reuters Mojo team, so does the iPhone live up to the hype?
Digital journalism – attempt first, succeed later
May 2, 2008 by Dave Earley
Filed under Journalists, Media Monologues, Online
Comments Off on Digital journalism – attempt first, succeed later
Digital media isn't about a perfect product straight out of the box, it's about taking the first step and just having a crack.
You've got NO EXCUSE
via News Videographer
I'm extremely impressed with these high school student journalists and the site. If they can do this, you really have NO EXCUSE.
Just have a go. Try your hand, you never know the amazing stories you can tell in new ways. You only learn through experience.
As part of that, there must be a culture within newsrooms that allows the freedom to fail in respect to digital media. You only fix mistakes by making them, and if people are going to learn the new skills they're going to have to not be afraid to make mistakes.
Encourage your newsroom to change.
The most basic things to encourage self-learning in are video and audio recording. Don't even worry about editing skills if people have zero experience or are discouraged. Just teach them to hit record. Give the result to someone else to edit/craft, and then give feedback.


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French Peak Road, Cave Junction, Oregon
$155,000- BedsActive
$155,000- BedsActive
French Peak Road, Cave Junction, Oregon 97523 - Magnificent acreage. 32.2 private acres. Part of historic Boswell gold mine complex. The property is beautifully treed, and close to Sucker Creek. It is zoned Serpentine. Serpentine zoning allows for a single family dwelling. Owner may carry. All information is deemed accurate but not verified or guaranteed; anyone considering the purchase of this property should perform their own investigation into the uses of the property. Listing agent is related to selling company's manager.
Listing Courtesy of John L Scott Real Estate Grants Pass


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(Repeats with no changes to text) (Reuters plans to stop publishing this item from April 1. For coverage of stories related to corporate finance on Eikon, please click on to retrieve the Investment Banking Top New page and to retrieve the IFR Top News page. For all Top News pages, click on. Thomson ONE users, please enter RT/TOP/DEALS in the News window to retrieve the Investment Banking Top News page. For the IFR Top News page, enter RT/TOP/NOW2. For comments and feedback, please contact firstname.lastname@example.com)
March 19 (Reuters) - The following corporate finance-related stories were reported by media:
* Cement makers Holcim and Lafarge, seeking to save their merger deal, are discussing a new leadership for the combined group which would give Lafarge's boss Bruno Lafont a lesser role, sources familiar with the matter said on Wednesday.
* Japan's loss-making electronics firm Sharp Corp plans to cut around 6,000 jobs, over 10 percent of its workforce, in a global restructuring that will cost over 200 billion yen ($1.7 billion), a person familiar with the plan said on Thursday.
* Spanish oil major Repsol has set the size of its hybrid bond issue at 2 billion euros ($2.1 billion), comprising two tranches of 1 billion euros each, a source close to the deal said on Wednesday.
* A unit of investment manager Neuberger Berman bought a 20 percent stake in hedge-fund firm Jana Partners LLC, valuing the New York-based firm at roughly $2 billion, the Wall Street Journal reported, citing a source familiar with the matter. (on.wsj.com/1B1v15E)
* Solar-energy company Sunrun Inc is powering up for a potential initial public offering later this year, the Wall Street Journal reported, citing sources familiar with the matter. (on.wsj.com/1BRCYxY)
* Deepak Fertilisers and Petrochemicals Corp has offered to sell its stake in Mangalore Chemicals and Fertilizers Ltd (MCF) to Zuari Group as it no longer sees merit in pursuing the battle for control of MCF, the Business Standard reported, citing sources familiar with the matter. (bit.ly/1Ex2hpZ)
For the deals of the day click on
For the Morning News Call-EMEA newsletter click on (Compiled by Shivam Srivastava in Bengaluru)


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Alaska, AK (PressExposure) June 09, 2014 -- With the growth of popularity level in the UFC zone, it can be well stated that the job sector of the mixed martial arts industry will surely lead to a different new level. The main area is related with the martial arts fitness and also the area of conditioning. This is also a leading call from MMA Conditioning Association, which will lead to the call by the zone of educating enthusiasts. The main aim is to lead by none other than educating enthusiasts in order to become the certified and this is related with the qualified conditioning coaches, especially designed for the martial artist.
The world class system, related with the UFC, is going to help you become the next MMA champion. Not only a champion, but you can also avail the same training, if you want to become someone else, apart from a champion. In case, at this current position, you are a MMA coach and want to enhance the skills at its best and also want to make more money, you can try and opt for the service from MMA Conditioning Association, to learn more about this field.
You will surely receive the title of ultimatefighting championships, after you have come across the experts from this site, as they are associated with this field for quite some time now. Even if you are fitness pro, still there are some special sessions available from this segment in order to increase the present monthly income and also this might help in offering a higher level of workout, related with the clients. On the other hand, in case you are a traditional martial arts owner, you might have to take some points to action fast. This can only be done after you have come across MMA Conditioning Association.
As the growth of martial arts careers is increasing at a fast pace with some of the most reliable salary option, people can try and go for the enthusiasts, related with the thirst to know more. You can easily avail some of the latest trainings along with the condoning strategies, when you will come across with the reliable site. The trusted sites are going to offer you with training options, which are provided by none other than leading professionals.


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6 Winch Place Ocean Reef 6027 WA
Renovated, Pool, Cul-de-sac & 700m to Beach
Lots of renovation in this spacious family entertainer with 3 living areas, separate study, stunning kitchen and plenty of floor to ceiling glass to take in the gorgeous backyard outlook
Fantastic new kitchen overlooks the pool and outdoors it offers 40mm stone bench tops, stainless appliances including 900mm oven, gas stove, range hood and dishwasher, stacks of overhead cupboards and big fridge space
Awesome outdoors with a huge wraparound alfresco, extensive paving, solar heated pool, easy care reticulated garden with plenty of room for the kids to play that includes a kids cubby and sandpit
Oversize master bedroom has a lovely ensuite, 3 door fitted sliding robe plus a separate built in robe. Kids bedrooms all feature built in robes. The study has a built-in robe and can be used as a nursery or 5th bedroom.
The bathroom with full size bath and the laundry have been beautifully renovated
Huge double garage has masses of extra space for motorbikes, trailers or storage, with side rear access for boats or caravans possible. Ducted air conditioning, 2 gas points, security doors and so much more!
Fabulous location in a quiet cul-de-sac, only 700m from the beach and proposed Ocean Reef Marina - stroll to beautiful parklands, local shops, Prendiville Catholic College, Ocean Reef High, St Simon Peter, Ocean Reef and Beaumaris Primary schools
Block size 801 sqm Built 1990
Call Shane for a private inspection or further information
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Peard & Associates
Hillarys WA, 6025
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Real Estate For Sale in Ocean Reef, WA 6027 priced between $520,000 and $960,000


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On the 6th day of Ganesh Chaturthi, we are giving you some special modak recipes that you can try out.
Modak's are very special to lord ganesh. It is believed that modaks and karanji are his most loved sweets.
This is Lord Ganesha's favorite sweet and hence, a must for this festival. Modaks are offered to the Lord and then distributed among devotees as prasad.
Try The Delicious Kalakand Sweet Recipe
Preparations of different types of modaks for Ganesh Chaturthi can be seen in various households across the country, especially in Maharashtra. For one sweet named modak, a variety can be achieved by altering the filling. So, why don't we go ahead and make our modaks?
Before going into the various types of modaks for Ganesh Chaturthi let us prepare the covering or shell (since it is shaped like one). This will remain common for all the varieties you try your hands at.
Modak Covering
For the modak covering, you will need:
• Rice flour – 1 cup
• Water – 1 cup
• Ghee – 2 tsps
Different Dal Recipes To Try On A Gloomy Day
In a heavy bottom deep dish, warm the water with the ghee.
When just about to boil, add the rice flour and stir well. Make sure that no lumps form.
It is a little difficult to stir this mix because the rice tends to expand and the mix gets considerably thick. It will soon start taking the form of dough.
Grease your palms with ghee and oil a working surface. Turn dough onto this greased surface and knead well. Kneading is important as you need to bring about an even textured dough with no cracks. It will have to be so even that it does not crack during steaming or else all the filling will spill out or it might get soggy.
Once you attain a smooth soft dough you can begin making your modak.
Types Of Modak Stuffing
Now let us move on to the different types of modaks for Ganesh Chaturthi. As mentioned earlier it is only the filling that makes the difference. Hence, we will be presenting a variety of stuffing here.
Ideally the stuffing should be prepared before the shell or covering. Doing so ensures that your dough is soft when stuffing.
• traditional modak: The traditional modak is made using coconut and jaggery and this is the one that is usually offered to lord ganesha. To prepare the traditional modak you will need the following:
Grated coconut – one cup
Jaggery – one cup
Nutmeg – a pinch
To prepare the filling mix coconut and jaggery in a heavy bottom pan and cook on a slow heat. The jaggery will melt and mix with the coconut evenly.
Once mixed turn off heat and add nutmeg. Some people also like to add saffron but it is your choice. Allow the mix to cool before you start stuffing.
Meanwhile place a steaming vessel to heat.
To make the modak take a lemon sized ball of the dough and roll it out evenly. Place a little stuffing in the center and gradually collect all the edges in the center to form a tip. If you can manage give it a pleated effect during the sealing process. It requires a bit of practice but is worth the effort.
When your steaming vessel is ready, place the prepared modaks in the steaming dish and cook for approximately 10 – 15 minutes.
Your steaming hot, juicy and aromatic modaks are ready!
• Dry Fruit Modak: As the name suggests this one is all about dry fruits. Crush dry fruits of your choice and cook them in jaggery instead of coconut. Add nutmeg, cinnamon or saffron – whichever one you prefer.
• Chocolate And Walnut Modak: A bit away from the Indian taste is the chocolate and walnut. Mix a cup of grated chocolate with crushed walnuts to make a very different type of modak for Ganesh Chaturthi.
• Mawa Modak: Take one cup mawa and cook in a heavy bottom pan till it turns pink and gives off fat. Mix half a cup of powdered sugar, half a cup of roasted rawa, a handful of grated coconut and a handful of raisins.
Superfast Modaks
If you have not got the time to prepare the filling and are looking for quick options try making use of readymade sweets you get at your local sweetmeat shop. Good options include boondi laddoo, dry fruit barfi, peda, milk cakes, besan laddoo and kaju katli.


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"The Other 9/11": Faith, Hope and the Media
What is the role and responsibility of the media in a post 9/11 era? How can we protect ourselves from images that leave us feeling helpless, fearful and insecure? In his presentation, Simon Cohen, managing director of the London-based Global Tolerance, drew out the example of the "other 9/11": September 11, 1906, when Gandhi first deployed his method of nonviolent resistance. By comparing both anniversaries and their legacies Cohen explored the unparalleled power of the media to influence perceptions about faith in society, for better or worse. Globalization and the social communications revolution linked with Twitter, Facebook, and YouTube empower us all, in new ways, to discover and promote faith's positive potential to contribute to a more just, tolerant, intercultural, and interreligious world.
Simon Cohen on the Other 9/11: Faith, Hope and the Media (EXCERPT ONE)
September 11, 2009
Simon Cohen on the Other 9/11: Faith, Hope and the Media (EXCERPT TWO)
September 11, 2009
"The Other 9/11": Faith, Hope and the Media
September 10, 2009


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Status: Closed
Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
Be the first PennLive.com user to add photos or videos of Horses LTD
Horses Ltd is located at 374 Worthington Mill Rd, Richboro, PA. This business specializes in Horse Training. Horses Ltd is open Mon - Fri, 9am - 5pm; Sat, 10am - 6pm; Sun, closed and accepts American Express, Cash, Mastercard and Visa.


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unwaveringly faithful to a political party; voting the straight ticket:
a brass-collar Democrat.
Tagged: b
Read Also:
- Brass-farthing
noun (Brit, informal) something of little or no value: his opinion isn't worth a brass farthing
- Brass-hat
a person in a high position, especially a top-ranking army or navy officer. Historical Examples Indiscretions of Archie P. G. Wodehouse noun (Brit, informal) a top-ranking official, esp a military officer A high-ranking officer in the military or other uniformed services (1890s+ British) Any high-ranking official; manager; chief; boss (1930s+) A high-ranking official, as in […]
- Brass-instrument
a musical wind instrument of brass or other metal with a cup-shaped mouthpiece, as the trombone, tuba, French horn, trumpet, or cornet.
- Brass-knuckles
a band of metal with four holes that fits over the upper fingers and that is gripped when a fist is made, used for increasing the effect of a blow with the fist. Historical Examples Andersonville, complete John McElroy Andersonville, Volume 2 John McElroy


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Wounded Warriors take on former Bucs in flag football lnkd.in/bXX-fuv
What a great win, and celebration in the locker room. Check it out!! lnkd.in/bNdiVtG
It's funny, after all these years I still wake up with GOOSEBUMPS on GameDay. Let's go STORM!! lnkd.in/dX7sED4
Thank you to all who have sacrificed so much for our freedom here in the US and abroad, both veterans and their... fb.me/45TFBJtxJ
Waking up to sad news of the passing of Chris's friend #WillSmith. Chris always spoke of how kind and generous... fb.me/4vMfvNauZ
I am giving 2 tickets against Jacksonville to the first person who messages me. I will leave the tix at will call. GO STORM!!
Thank you to The Wounded Warrior Foundation, and The Tampa Bay Storm for allowing me to be a part of a great night. …lnkd.in/dPZYVzn


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In My Sleep Trailer (2010)
"Sleepwalking Can Be Deadly" 23 April 2010 Thriller, Mystery 90 mins
Marcus (Philip WInchester) wakes up in the middle of a cemetery half naked and has no idea how he got there. Urgent questions pound inside his head. "Where was I last night? Who was I with?" Marcus suffers from parasomnia, a rare sleep disorder which causes him to do things in his sleep which he cannot remember the next day. His situation takes a horrifying turn when Marcus wakes up covered in blood with a knife at his side. Then, the wife of his best friend is found stabbed to death. Marcus is terrified to put together the pieces - could he have murdered her in his sleep to hide a dark secret between them? Mysterious phone calls make Marcus believe someone is watching him. He investigates his own nocturnal activities, desperate to figure out what happens after he goes to sleep at night. His journey to discover the truth ends in a shocking discovery.
Watch the official In My Sleep 2010 trailer in HD below or find more In My Sleep videos on Vidimovie.


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Film & Video - List of Companies, Distributors, Suppliers, Manufacturers, Importers, Exporters in Canada
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VideoSharks is a company located at 294 Villeneuve Ouest, Montreal, Quebec. Their main product is online explainer videos. Their wide range of experience in video production and ta...
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Dia's wedding photography is refreshing, spontaneous, and emotional. His style is clean with a modern sensibility. He portrays special moments via a unique blend of photojournalism...
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At Viva Media, the foundation of our business is one built on passion. We love what we do, we're good at it, and it shows. For more, call 1 647-749-8842
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Currently 0.00/512345 Rating: 0.0/5 (Total votes: 0)
Rating: 0.0/5 (Total votes: 0)
What's New in This Release:
· This little update to Birdbrain fixes the authentication issue that prevented users with two-factor authentication from being able to authenticate in Birdbrain. We say NO MORE. Just because you love safety does not mean you can't love Birdbrain. Have at it.
· We've also made some improvements to the drawer navigation (so it no longer seems like we'd removed the key feature of the app).
What's New in 2.6:
· Birdbrain has undergone a number of huge changes to drastically improve the performance of the app and to get rid of pesky crashes.
» show more


text:
GraphicRiver Clocks 6060533
Category: Stock Vector | Comment: ( 0 )
GraphicRiver Clocks 6060533
Stock Vector \ Objects \ Man-made objects
Ringer \ Section \ Ring \ Report \ Isolated \ Light \ Sector \ Watch \ Timer \ Time
Stock Vector - Clocks 6060533 by Ennessy @ GraphicRiver
Stock Vector \ Objects \ Man-made objects
Graphics Files Included: Photoshop PSD, Transparent PNG, JPG Image, Vector EPS, AI Illustrator ; Layered: No ; Minimum Adobe CS Version: CS .
different detailed clocks (hourglass, wall clock, desk clock) and stopwatches on a white background
Sand Clock; Eyes Icons; Decorative Trees; Woman Dresses on Hanger; Trees of Four Seasons; Leaves Set; Vector Leaves.
Keywords: alarm, analogue, arrow, bell, button, call, clock, data, digits, diod, element, font, hour, hourglass, icon, indicator, isolated, light, report, ring, ringer, section, sector, set, stopwatch, symbol, time, timer, watch, web.
Download Link


text: Flat Roof Repair in Seasalter, Whitstable The job consisted of 2 flat roof replacements, over a garage and a rear extension. The existing "felt roof" covering is being stripped off and carted away and the decking inspected and replaced as necessary. On this particular job, around 50% of the deck had failed due to water leaks. The deck was replaced with OSB3 smart ply, other options could be wbp (water boiling point) ply, marine ply or fully bonded to flat roof insulation boards as a warm roof system. Replacement System The new system comprises a 3 layer, torch-on, high quality … [Read more...]


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