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All Items : Collector Plates : Pre 1980 item #1183884 (stock #safn-4020)
B&G BING & GRONDAHL Mother's Day 1971 Collector's Plate Mors Dag 1971 A mother cat carries her kitten over a bed of hay. Plate is in its original box, no paperwork. The back is marked with the usual B&G Copenhagen Denmark stamp and 8000/9371 and the blue artist mark. No defects of any kind. perfect condition. Plate measures 5 7/8" in diameter.
All Items : Collector Plates : Pre 1980 item #1183887 (stock #safn-4021)
B&G BING & GRONDAHL Mother's Day 1975 Collector's Plate Mors Dag 1975 A mother deer and her 2 fawns. Plate is in its original box, no paperwork. The back is marked with the usual B&G Copenhagen Denmark stamp and 9375 and the blue artist mark. No defects of any kind. perfect condition. Plate measures 5 7/8" in diameter.
Pretty hand painted 11th birthday girl figurine by Enesco. One from their growing up girls series. She is 5 1/4" tall and is in perfect condition. She wears a delicate mint green dress with a small rosebud on the birthday cake she is holding. She had pretty brown hair with matching green bow, and the #11 in gold on the cake. Dated 1982. These make gret cake toppers for their cakes, then a collectible keepsake all in one. I have two with brown hair, and I have a blonde listed also.
All Items : Dinnerware : Franciscan : Pre 1970 item #1185667 (stock #safn-4108)
Sherrys Antiques* Collectibles* Frivolous Necessities
$12.00 ea
$12.00 ea
Franciscan Hacienda Green Earthenware 5 1/4" fruit, sauce, or dessert bowl by Franciscan, in the Hacienda Green design. This bowl shows gentle use and is in Very nice condition with no visible wear or damages. Harder size to come by. It measures 5 1/4" diameter and 1 1/2" deep. I will be listing several pieces to this pattern. Most are marked with the older TV style backstamp and some have the later Earthenware stamp. Made from 1964-1983. Please specify the backstamp if this makes a difference t...
All Items : Dinnerware : Franciscan : Pre 1970 item #1185739 (stock #safn-4113)
Sherrys Antiques* Collectibles* Frivolous Necessities
$12.00 set
$12.00 set
Desert Rose was first introduced in 1941 by Gladding, McBean and Co. THis is a cup with matching saucer and have the TV mark. all are in excellent condition. Good clear pattern and free from any defects. Price is for one cup with matching saucer. I have 4 perfect sets available. Price is for one cup with matching saucer.
All Items : Glassware : Depression : Companies : Pre 1980 item #1185750 (stock #safn-4116)
EAPC Early American Prescut Star of David divided relish tray or dish from Anchor Hocking. This piece has a scalloped top edge. Dish is about 10 inches long including the handles and about 6 inches wide. There are no chips, cracks or repairs. I have 5 available. Price is per dish.
1930s Taylor, Smith & Taylor TST Pottery creamer. Decorated with a Petit point Bouquet Design, like needlepoint, cross stitch or tapestry. Relief pattern rim with gold edge trim. Stamped Taylor, Smith & Taylor. Excellent Condition - Nice Bright Decorations - Absolute Minor Wear. Please check out the other pieces I will be listing to this lovely pattern. Save on shipping by ordering quanities. Sorry picture is not so good on this one. It is in beautiful condition though.
This hare will never win the race! He has paused to daydream and smell the flowers! Cute figural ceramic turtle planter would be perfect for a nursery. Pretty soft pastel highlights. Measures 3" tall and is 6" long. No chips or damages. Unknown maker.
All Items : Glassware : Depression : Companies : Hocking : Pre 1960 item #1191772 (stock #safn-4161)
Lovely RARE HOCKING AMBER BOOPIE GLASS 4 oz GOBLET from the 1950's. Rich Amber - Gold color bowl with clear foot and stem base surrounded with bubbles. These hold aprox. 4oz of your favorite beverage. Aprox. 3.5" tall, top rim aprox, 3.75" in diameter, base aprox. 2.5" in diameter. Fantastic condition with not the slightest wear or flaw. I just have two right now. Price is for each.
Finsbury Fine Bone China Thimble. Made in England 1" tall. Says "Congratulations on your Baby" and has a little blue bird, and accented with gold trim. Inside reads: Finsbury Fine Bone China. Made in England Excellent condition. I have 5 available. Price is per thimble. A great gift.
All Items : Breweriana : Pre 1960 item #1195615 (stock #safn-4229)
Vintage shot glass made of glass with thick bottom.Graphics of an English chap, with the roving eye. On the back it says "I say Jolly What" Perfect condition with the exception that the lettering is a little faint. I believe Anchor Hocking made these.
Degenhart Glass Orange Pink Bird Open Salt Cellar Dip. This is a salt dip shaped like a bird with a berry or marble in it's beak. It is a pinkish orange salt cellar. It measures about 3 inches long. Made by either Boyds or Degenhart. No chips, cracks or repairs.
Sliding into the New Century With Joy (Porcelain Sled Ornament)Number Of Item: 547778. Production Status: Limited Edition Porcelain Sled Ornament from 1999 -Included Items: Original Box Description: A Girl on a Sled with Her Puppy on a Porcelain Sled. Very Cute!!! This Precious Moments Item is in excellent condition and appears to never been used. All original packaging & box.
All Items : Glassware : Depression : Companies : MacBeth-Evans : Pre 1940 item #1197183 (stock #safn-)
Sherrys Antiques* Collectibles* Frivolous Necessities
$12.00 ea
$12.00 ea
Pink american Sweetheart pattern cereal bowl by MacBeth-Evans Glass Company, 1930-1936. 5 7/8" wide bowl is 1 5/16"deep. Excellent condition. I have 2 available. Price is per bowl.
All Items : Glassware : Depression : Companies : Federal : Pre 1940 item #1197406 (stock #safn-4275)
Marigold Carnival depression glass Normandie Bouquet and Lattice cereal bowl by Federal Glass 1933-1940. Measures 6 1/2" diameter. Perfect condition with no damages. I just have 1, but have other pieces in this pattern in my catalog.
Gorgeous reproduction of an antiqued gold filigree necklace woth tiers of chains filigree and blue saphire cabochons. Adjustable clasp will fit up to a 19" neckline. Like new, and now the newer generation refers to it as a Boho or Bohemian style. You will love it.
A sweet little rabbit for your collection or for Easter decorating. His ears are long so he may be a jack rabbit. Soft glaze. Measures 4 1/2" tall to the tip top of his ear. Perfect condition. Marked on the bottom, Foreign.
Heavy china like Hall China would make. Not sure who made this but it's reaaly cool and vintage. Forest green bowl w/ lid. Lid has a fish on top. No chips or breaks. About 3 1/2" across About 3 1/4" Tall (including fish on lid)


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Discussion in 'General Off-Topic Chat' started by Westside, May 29, 2011.
very powerful words...
And yet this changes nothing, sadly. Though it's great that people are standing up. He made a great speech. One of the best I've ever heard on such a subject.
This particular line made me choke a bit: "Without racism, soldiers would realize that they have more in common with the Iraqi people then they do with the billionaire that send us there."

subset: commoncrawl


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Grb2-associated binder 1 (Gab1), a scaffolding adaptor protein, plays an important role in transmitting key signals that control cell growth, differentiation and function from multiple tyrosine kinase receptors. The study was designed to investigate the role of endothelial Gab1 in angiogenesis and underlying molecular mechanisms.
Using cre-loxp technology, we generated endothelial-specific Gab1 knockout (Gab1-ecKO) mice. Gab1-ecKO mice are viable and showed no obvious developmental defects in the vascular system. To analyze the role of Gab1 in postnatal angiogenesis, we used hindlimb ischemia and Matrigel plug models. We found that loss of endothelial Gab1 in mice dramatically impaired postnatal angiogenesis. Gab1-ecKO mice had impaired ischemia-initiated blood flow recovery, exhibited reduced angiogenesis and were associated with marked limb necrosis. We further observed significant EC death in the ischemic hindlimb of Gab1-ecKO mice. Matrigel plug assay showed that hepatocyte growth factor (HGF)-mediated angiogenesis was inhibited in Gab1-ecKO mice. In vitro studies showed that Gab1 was required for HGF-induced EC migration, tube formation and microvessel sprouting. Mechanistically, HGF stimulated Gab1 tyrosine phosphorylation in ECs, leading to activation of ERK1/2 and Akt, which are angiogenic and survival signaling.
Gab1 is essential for postnatal angiogenesis through mediating angiogenic and survival signaling.
Angiogenesis, the formation of new blood vessels from existing vascular network, begins with the activation, migration, and proliferation of endothelial cells (ECs) in the existing vessels.1 Many growth factors, including vascular endothelial growth factor (VEGF), are shown to regulate EC function and angiogenesis.2 Besides VEGF, hepatocyte growth factor (HGF) is a potent angiogenic factor in vivo and stimulates vascular EC migration, proliferation, and organization into capillary-like tubes in vitro.3–6 HGF binds its receptor c-Met and stimulates c-Met kinase activation, which triggers transphosphorylation of c-Met and downstream signaling events.7, 8 HGF/c-Met pathway has emerged as a promising therapeutic target to promote or inhibit angiogenesis.7–9 However, the mechanisms by which HGF mediates angiogenesis have not been fully understood.
Grb2-associated binder protein 1 (Gab1), a member of the insulin receptor substrates 1 (IRS1)-like multi-substrate docking protein family, is highly expressed in vascular ECs. Gab1 is found to be directly or indirectly recruited to numerous activated receptor tyrosine kinases (RTK), including c-Met and VEGF receptor 2 (VEGFR2, also named as Flk1 or KDR).10–15 Upon phosphorylation by RTK, Gab1 then recruits and activates phosphatidylinositol 3-kinases (PI3K)/Akt and protein tyrosine phosphatase SHP2/ERK1/2 pathways, which are crucial to cell proliferation and differentiation.13, 14, 16–23 However, the role of Gab1 in growth factors/RTKs-mediated angiogenesis remains largely unclear.
Gab1 deficiency in mice is early embryonic lethal due to heart and placental defects.24, 25 In order to directly explore the role of Gab1 in angiogenesis, we generated Gab1 endothelial-specific knockout mice (Gab1-ecKO). Using hindlimb ischemia, Matrigel plug and tumor angiogenesis models, we showed defective postnatal angiogenesis in Gab1-ecKO mice. We further found that Gab1 was required for EC survival in vivo and in vitro under stress conditions. Moreover, HGF-induced Akt and ERK1/2 activation and cell survival were impaired in Gab1 deficient ECs. Thus our data demonstrate that Gab1 is crucial for postnatal angiogenesis through mediating angiogenic and survival signaling.
Gab1flox/flox mice (C57BL/6J background) described previously26 andTie2-Cre transgenic mice from Jackson laboratory (C57BL/6J background, stock number 008863) were used. All protocols for animal experiments were approved by the University Committee on Animal Resource of University of Rochester.
Hindlimb ischemia was generated by resection of the femoral artery in male WT or Gab1-ecKO mice as previously described.27 Laser Doppler perfusion imaging was used to record perfusion of both right and left limbs at different time points as indicated.
An expanded methods section is available in supplemental data including hindlimb ischemia model, Laser Doppler perfusion imaging, capillary density analysis, TUNEL staining, in vivo Matrigel plug angiogenesis assay, tumor angiogenesis, aortic ring assay for ex vivo angiogenesis, isolation of mouse lung ECs, cell culture and siRNA transfection, wound closure cell migration, Boyden chamber migration, capillary-like tube formation, Western blot analysis, immuno-fluorescent staining and microscopy, MTT cell viability assay, and data statistic analysis.
In order to explore the role of endothelial Gab1 in vivo, we generated Gab1 endothelium-specific knock-out mice. Gab1flox/+ mice were bred into Tie2-Cre/+ transgenic mice that express Cre in ECs. Tie2-Cre;Gab1flox/flox (Gab1-ecKO) mice were generated by male Tie2-Cre/Gab1flox/+ and female Gab1flox/flox intercross. Gab1-ecKO mice were viable and were born at the expected mendelian ratio (Supplemental Table I). Supplemental Figure I in the Supplemental Data shows the genotyping results for Gab1-ecKO mice and Gab1flox/flox wild-type (WT, control) littermates. Gab1-ecKO mice had no obvious defects on body weight and vascular development. Lung ECs were isolated. EC morphology was normal (Supplemental Figure II) and confirmed with endothelial specific markers PECAM1 (CD31) and VE-cadherin immunostaining (Supplemental Figure III). Immunobloting analysis showed a significant decrease of Gab1 expression in ECs isolated from Gab1-ecKO mice relative to that from WT mice (Supplemental Figure IV). Co-immunostaining Gab1 with EC specific marker vWF in mouse hindlimb muscles further confirmed EC specific Gab1 deficiency in Gab1-ecKO mice (Supplemental Figure V), while relative low expression of Gab1 in muscle fibers in both Gab1-ecKO mice and WT mice were observed. Moreover, we found that hindlimb vasculature and retina vascular network in adult Gab1-ecKO mice are similar to those in WT mice (Supplemental Figure VI and VII). Collectively, our results indicate that Gab1-ecKO mice have no obvious developmental defects in the vascular system.
To examine the role of endothelial Gab1 in postnatal angiogenesis, we used a mouse hindlimb ischemia model. The gastrocnemius blood flow was measured using a deep penetrating Laser Doppler probe. As shown in Figure 1A and 1B, the ratio of blood flow of the right limb relative to the left limb was 100% before surgery. After surgery to the right limb, the ratios dropped by 80% in all groups of WT and Gab1-ecKO mice. Blood flow in the gastrocnemius muscle in WT mice recovered in a time dependent manner. However, Gab1-ecKO mice failed to recover the blood flow, suggesting that Gab1 may play a role in postnatal ischemic angiogenesis. Moreover, Gab1-ecKO mice showed marked necrotic limb at 14 days after the femoral artery resection (Figure 1C), which is consistent with the defective recovery of blood flow. We used a clinical scoring system to assess lower-limb function and tissue salvage after surgery.27 As shown in Figure 1D, the deficiency of endothelial Gab1 was associated with severe tissue ischemia after surgery.
To characterize the ischemia-initiated angiogenesis, we quantified the capillary density of gastrocnemius muscle fibers from both WT and Gab1-ecKO mice before and after the hindlimb ischemia. As shown in Figure 1E, ischemia-initiated angiogenesis resulted in increased capillary density relative to the baseline in WT mice, while Gab1-ecKO mice had dramatically lower capillary density, less than that in the nonischemic control side (Supplemental Figure VIII shows color images of capillary CD31 staining). Taken together, these results established a critical role of endothelial Gab1 in ischemia-initiated angiogenesis.
Notably, Figure 1E showing a dramatically decreased capillary density in Gab1-ecKO mice suggests the potential involvement of EC survival in vivo under hypoxia which results in defective angiogenesis and vascular regression. To directly examine the role of Gab1 in cell survival, we performed TUNEL staining on tissues from Gab1-ecKO mice and WT mice after hindlimb ischemic surgery. Endothelial marker gene von Willebrand Factor (vWF) and nuclear DNA DAPI staining were also performed to identify ECs and cell nuclei. As shown in Figure 2, there were significantly more TUNEL-positive ECs in gastrocnemius muscle in Gab1-ecKO mice than in those of WT mice after ischemia. EC apoptosis in the arterioles were further comfirmed by triple immunostaining with TUNEL, vWF and smooth muscle alpha-actin (Supplemental Figure IX). It is worth noting that EC death started as early as 2 days (Figure 2A and 2B) and persisted at 7 days (Figure 2C and 2D) after ischemia in Gab1-ecKO mice, revealing a pivotal role of Gab1 in EC survival under ischemic stress.
It has been reported that Gab1 is involved in VEGF-induced EC survival.28 HGF is also a potent survival growth factor.29 We observed that expression of both VEGF and HGF were increased in ischemic hindlimb muscles (Supplemental Figure X). Interestingly, we found that HGF strongly induced Gab1 tyrosine phosphorylation in ECs (Supplemental Figure XI). To examine whether Gab1 mediates HGF-induced EC survival under stress conditions, we used the MTT cell viability assay and mouse lung ECs isolated from Gab1-ecKO mice and WT mice. Under serum starvation for 24 hours, cell viability of Gab1 deficient ECs dropped by almost 55% relative to the control WT ECs (Figure 2E). While HGF significantly protected WT ECs from death, it failed to maintain EC survival in Gab1 deficient ECs (Figure 2E). Less extent effects were observed when VEGF was used to protect ECs from death (Supplemental Figure XII). We also examined the levels of cleaved caspase 3 for the induction of apoptosis, and found that increased active caspase 3 in response to serum starvation was much higher in Gab1 deficient ECs than that in WT ECs (Supplemental Figure XIII). These data show that Gab1 is required for EC survival under serum starvation.
In order to dissect the role of Gab1 in HGF-induced angiogenesis in vivo, we used the Matrigel plug model, in which Matrigel mixed with HGF was subcutaneouslyinjected to Gab1-ecKO mice and WT mice. Matrigel plugs were harvested one week later, and stained for CD31. As shown in Figure 3A, deficiency of endothelial Gab1 in mice significantly reduced invading ECs and the density of microvessels in the Matrigel plugs (Supplemental Figure XIV shows color images of capillary CD31 staining). Hemoglobin concentration in the Matrigels was measured to assess the functional vessels in the Matrigel plugs. As shown in Figure 3B, hemoglobin concentration was much lower in Matrigel plugs implanted in Gab1-ecKO mice than that in Gab1-WT mice, consistent with the results of CD31 staining for microvessels in Matrigel plugs. These data demonstrate that Gab1 is essential for HGF-induced angiogenesis in vivo. Relatively less effects of endothelial Gab1 deficiency on VEGF-induced angiogenesis were observed by the Matrigel plug assay using Gab1-ecKO mice and WT mice (Supplemental Figure XV). In addition, we examined the role of Gab1 in tumor angiogenesis and found that endothelial Gab1 deficiency inhibited the tumor angiogenesis and tumor growth (see Supplemental Figure XVI and XVII).
To further investigate the role of Gab1 in angiogenesis, we performed the aorta ring ex vivo angiogenesis assay, in vitro angiogenesis capillary-like tube formation and EC migration. In the aorta ring assay, we found that the number of sprouting microvessels from the aorta rings isolated from Gab1-ecKO mice in the presence of HGF was dramatically reduced (Figure 4A and 4B). In the capillary-like tube formation assay, we used mouse lung ECs isolated from Gab1-ecKO mice and WT mice. As shown in Figure 4C and 4D, the capillary-like tube formation in the presence of HGF was significantly enhanced in WT ECs, while HGF-induced capillary-like tube formation in Gab1 deficient ECs was impaired. Cell migration was measured by the modified Boyden's chamber method and wound-healing migration assay. In the modified Boyden's chamber method, HGF-induced EC migration was impaired in Gab1 deficient ECs compared to that in WT ECs (Figure 4E). Less effects of endothelial Gab1 deficiency on VEGF-induced EC migration were observed (Supplemental Figure XVIII). In the wound-healing migration assay, Gab1 deficient ECs failed to close the wound areas in the presence of HGF (Figure 4F and 4G). In addition, we infected adenoviruses encoding mouse Gab1-WT or LacZ (control) in Gab1 deficient ECs. Introducing mouse Gab1 but not LacZ rescued the ability of wound closure for these ECs in response to HGF (Figure 4H–J).
The PI3K/Akt and ERK signaling pathways are involved in EC survival and in vitro angiogenesis. To investigate how Gab1 mediates HGF-induced EC survival and migration, we assessed the activation of Akt and ERK1/2 in response to HGF stimulation using human umbilical endothelial cells (HUVECs) transfected with Gab1 siRNA or scrambled siRNA (control). Gab1 siRNA efficiently reduced the level of Gab1 expression in HUVECs (Figure 5A). Knockdown Gab1 substantially decreased the levels of Akt and ERK1/2 phosphorylation in response to HGF (Figure 5A–C). We also used Gab1 deficient ECs to examine HGF-induced Akt and ERK1/2 signaling and observed similar results (Figure 5D and 5E). Gab1 deficiency did not affect HGF receptor c-Met tyrosine phosphorylation (Supplemental Figure XIX). Moreover, we infected HUVECs with various Gab1 mutants to dissect the Gab1 signaling pathways. The overexpression of adenoviral Flag-Gab1-ΔPI3K (three PI3K binding sites tyrosine were replaced by phenylalanine) in HUVECs greatly inhibited HGF-mediated Akt activation but not ERK1/2 activation, while the overexpression of adenoviral Flag-Gab1-ΔSHP2 (two SHP2 binding sites tyrosine were replaced by phenylalanine) in HUVECs blocked HGF-mediated ERK1/2 activation but not Akt activation (Figure 5F–H).Taken together, these results establish a critical role of endothelial Gab1 in angiogenic and survival signaling.
The novel finding of the present study is that endothelial Gab1 plays an essential role in postnatal angiogenesis in mice. Mechanistically, we found that Gab1 is critical for mediating HGF angiogenic signaling and survival of ECs under stress conditions in vivo and in vitro. To the best of our knowledge, this is the first study that revealed a key role for Gab1 in angiogenesis and HGF-mediated signaling in ECs.
Several genetic and biochemical studies have shown that Gab1 is involved in mouse development, oncogenic transformation and metabolism.24–26, 30 In particular, Gab1 deficient mice showed developmental defects in the heart, placenta, and skin, and died between E13.5 and E18.5.25 However, the role of Gab1 in vascular endothelial biology and angiogenesis have not been explored. Using cre-loxp system, we first generated Gab1-ecKO mice, which are viable and have no obvious vascular development defects. Using three angiogenic models including hindlimb ischemia, Matrigel plug and tumor angiogenesis in Gab1-ecKO mice, we showed that Gab1 regulates postnatal angiogenesis in vivo. Specifically, we found that ischemia-induced vessel growth and blood flow recovery were severely impaired in Gab1-ecKO mice compared to those in WT mice. HGF-mediated EC infiltration and microvessel growth in the implanted Matrigel plugs were blocked in Gab1-ecKO mice. Moreover, tumor angiogenesis and growth were inhibited in Gab1-ecKO mice. In agreement with our in vivo results, our in vitro studies showed that Gab1 is required for HGF-induced microvessel sprouting, EC migration and tube formation, which are essential components in angiogenic processes in vivo. Taken together, our studies clearly demonstrate an indispensable role of endothelial Gab1 in mediating angiogenesis in vitro and in vivo.
It has been shown that tissue ischemia/hypoxia induces expression of growth factors, which stimulates EC proliferation and migration resulting in neovascularization.31 Meanwhile, ischemia/hypoxia blocks nutrition supply and generates reactive oxygen species that may promote cell death, suggesting that EC survival could play important roles in ischemia-initiated angiogenesis. In this study, we observed that ischemic hindlimb in Gab1-ecKO mice underwent severe toe necrosis and had much lower capillary density in the ischemic limb than that in the nonischemic control, indicating that Gab1-ecKO mice not only have impaired angiogenesis but also show potential regression of preexisting vasculature. Therefore we reasoned that a Gab1-mediated cell survival mechanism may maintain preexisting vasculature and regulate angiogenesis under ischemic conditions. Indeed, our TUNEL and vWF double staining studies demonstrate that there is significant EC death in the ischemic hindlimb of Gab1-ecKO mice. In particular, we detected vascular EC death in the ischemic hindlimb of Gab1-ecKO mice, starting as early as 2 days and persistent at 7 days after femoral artery resection. To our knowledge, this is the first evidence showing the potential role of EC survival in hindlimb ischemia–induced angiogenesis and the role of Gab1 in EC survival in vivo. Consistent with these observations, we found that ECs isolated from Gab1-ecKO mice are more sensitive to starvation-induced cell death than those isolated from WT mice. The levels of cleaved caspase 3, a critical executioner of apoptosis, were enhanced in ECs isolated from Gab1-ecKO mice under starvation conditions, further supporting the concept that Gab1 acts as a survival factor against EC death. Collectively, our results indicate that Gab1-mediated EC survival is involved in ischemia-initiated angiogenesis.
Akt and ERK1/2 activation have been shown to regulate cell migration and survival signaling pathways.32 Several studies have suggested that Akt and ERK1/2 pathways are involved in HGF-mediated angiogenesis in vitro.33, 34 Gab1, an adaptor protein, plays an important role in mediating growth factors-induced activation of Akt and ERK1/2 through recruiting PI3K and SHP2 in a tyrosine phosphorylation-dependent manner.35, 36 However, the role of Gab1 in HGF-mediated signaling in ECs remains unclear. We first found that HGF strongly stimulated Gab1 tyrosine phosphorylation in ECs. Using Gab1 deficient ECs isolated from Gab1-ecKO mice and Gab1 siRNA knocked-down HUVECs as well as Gab1 phosphotyrosine mutants, we further found that Gab1 mediated HGF-induced Akt and ERK1/2 activation in ECs. The data provide signal mechanisms by which Gab1 mediates growth factors-induced angiogenesis.
Many growth factors are implicated in the processes of angiogenesis in vivo. Our results showing that VEGF and HGF are induced in response to ischemia, suggest that both VEGF and HGF are involved in ischemia-induced angiogenesis. In vitro studies have shown that Gab1 was tyrosine phosphorylated and mediated angiogenesis in vitro in response to VEGF. Consistent with this concept, we found that VEGF-induced angiogenesis was affected in Gab1-ecKO mice. However, we observed much stronger Gab1 tyrosine phosphorylation in ECs in response to HGF than that in response to VEGF. We further found that HGF-induced angiogenesis in vitro and in vivo was severely impaired in Gab1-ecKO mice. Our results suggest that Gab1 signaling is more important in HGF-mediated angiogenesis than VEGF-mediated angiogenesis.
It has been shown that enhanced secretion of cardiac HGF from myocardial infarct regions is associated with an attenuation of ventricular enlargement and an improvement in cardiac function.37 In fact, administration of recombinant HGF promotes angiogenesis in ischemic hindlimb and heart in various animal models.6, 38, 39 In this study, we uncover an essential role for Gab1 in ischemia-, tumor- and HGF-mediated angiogenesis. Our findings may have clinical implications and suggest that blocking Gab1 function results in the inhibition of tumor angiogenesis and tumor growth, and enhancing Gab1 signaling could be a potential therapeutic strategy to improve ischemic diseases.
Sources of Funding
This study was supported in part by the American Heart Association Postdoctoral Fellowship (to W.W.), Postdoctoral Fellowship (to C.H.H), NIH grant HL076754 and HL097593 (to M.K.J.) and Grant-In-Aid Award 0755916T (to Z.G.J.), the American Diabetes Association Thomas R. Lee Award 1-06-CD-13 (to Z.G.J.), and NIH grant HL80611 (to Z.G.J.).
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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There is a power in quietly taking the steps forward to your dreams without making a fuss. There is a strength that is willing to do the work, whatever it takes, a humility that says no task is beneath me. There is a peace that carries through difficult situations with grace, never complaining. It seems like I'm developing a distaste for unnecessary drama, for artifice, for show, for charade. Maybe it's the current state of our national affairs, maybe it's because I used to be so dramatic and fall apart all the time, circling the drain of my own private hostage crisis, dizzy from my own emotional ups and downs. I don't want a corporate solution to a personal problem. I want the world to care about every single being, to take the problems of each and every one of us for real. I want us to really listen and see the bare truth of all the hurt and all the beauty in each and every human being. I want us to brave enough to let that heartache in, I want us to be enlightened enough to evolve into the force of change that we can be. I want us to wake up to the truth of who we are, or who we could be, who we are meant to be, if we only drop the illusion of separateness, drop the artificial otherness, let go of the false pretense of hierarchy. I want us to rise up and go so high that low is just not an option anymore. Am I dreamer? I hope I'm not the only one.
#practiceyogachangeyourworld #onebreathatatime
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New Jersey: Oct. 21-23
Puerto Vallarta: 18-20
Toronto: Nov. 4-6
Practice with me!
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Chinese authorities are reported to have arrested at least five artists on the Chinese mainland who have shown support for pro-democracy protests in Hong Kong.
The Chinese authorities are determined to prevent pro-democracy rallies in Hong Kong from spilling across the border into the mainland, so they have detained at least 10 people, including a group of artists who attended a poetry reading inspired by the protests in Hong Kong.
On 11 October 2014, three artists were taken away from Beijing's Songzhuang art enclave. According to the BBC, the arrests were likely connected to the artists' support of the Hong Kong protests. The artists are the 42-year-old painter from Shandong Province, Zhang Haiying, the 36-year-old poet and writer Ouyang Xiajie, and the 39-year-old Tibetan artist and painter Kuang Laowu.
Previously, allegedly also because of their solidarity with the Hong Kong occupation, Beijing Songzhuang police arrested the well-known poet Wang Zang and the artist Cui among six other people.
On 30 September, Wang Zang had posted the above picture of himself on Twitter, raising his middle finger and holding the umbrella – a symbol of solidarity adopted by the protesters demanding open nominations for Hong Kong's chief executive.
Police apprehended Wang Zang in front of his Beijing home on the night of 1 October 2014, searched his apartment and confiscated a light blue umbrella he had used in a picture posted on Twitter, as well as a computer, a router and other materials, his wife Wang Li told The Associated Press.
Lawyer Sui Muqing said his client was detained for "provoking trouble," which he said carries a sentence of up to three years in prison.
At least another 20 people in mainland China have been detained for supporting the protesters, including posting pictures and messages online showing solidarity and planning to travel to Hong Kong to join protesters, according to human rights group Amnesty International. Another 60 have been called in by police for questioning.
Voice Project / AP – 10 October 2014:
Chinese artist arrested after tweeting picture with umbrella
Chinese police detained a well-known poet and seven other people ahead of a poetry reading planned in Beijing to support pro-democracy protesters in Hong Kong, relatives of the detained said.
» Artnet News – 13 October 2014:
Artists Arrested for Supporting Hong Kong Protests
By Zoe Li
» BBC News – 11 October 2014:
Beijing: Well-known human rights activist Guo Yu in detention


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Published: Monday 8th December 2014 by The News Editor
The Duke of Cambridge will begin his American tour by branding the illegal wildlife trade as one of the most "insidious forms of corruption" in the world.
William and Kate are due to arrive in New York later this evening for a three-day visit that has generated "incredible" excitement among New Yorkers.
The second in line to the throne has been campaigning to save threatened animals for a number of years and will tell delegates at a Washington conference tomorrow some animal parts are worth more then their weight in gold.
The royal couple have a varied itinerary that will see the Duke meet US president Barack Obama, the Cambridges rub shoulders with celebrities at an NBA basketball match and the Duchess visit a Harlem based organisation supporting families.
They will also pay their respects to the thousands who died during the 9/11 terrorist attacks by visiting the World Trade Centre's Memorial and Museum.
The royal visit has already captured the imagination of New Yorkers said Danny Lopez, British consul general, New York.
Describing the atmosphere since the visit was announced he said "over the last few weeks there has been incredible interest" and a huge "level of excitement" from people who want to support or be a part of the royal engagements.
William will give a major speech in Washington tomorrow after meeting the American leader at the White House and the Vice President Joe Biden.
He is passionate about trying to preserve the planet's most endangered animals and habitats and founded the umbrella organisation United for Wildlife to try and tackle the issue and other problems.
At the World Bank Group's International Corruption Hunters Alliance Conference the Duke is expected to describe the trade in elephants tusks, rhino horns and other animal parts as "one of the most insidious forms of corruption and criminality in the world today".
Highlighting the scale of the problem William will say "some endangered species are now literally worth more than their weight in gold."
William will stress that in China, a huge market for endangered animal parts, the wholesale price of ivory has increased from five US dollars (£3) to 2,100 US dollars (£1,346) per kilogramme in 25 years and poaching has increased as a result.
The Duke will issue a call to action urging the world to act now: "Our collective goal must be to reduce the wildlife trade by making it harder: denying traffickers access to transportation, putting up barriers to their illegal activities, and holding people accountable for their actions.
"Those who look the other way, or spend the illicit proceeds of these crimes, must be held to account."
William will be joined by former foreign secretary William Hague, who hosted the Government's London Conference on the Illegal Wildlife Trade in February, attended by the Duke, Prince Harry and the Prince of Wales who spoke at the event.
Wildlife crime also poses a threat to world security as it "exacerbates sources of conflict" the Duke will say, and he is scathing about traffickers in animal parts: "Together they loot our planet, to feed mankind's ignorant craving for pets, trinkets, cures and ornaments derived from the world's vanishing and irreplaceable species."
Published: Monday 8th December 2014 by The News Editor


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The Fort William Henry Museum and Restoration has added a second ghost tour for this season to appeal to the surging popularity around paranormal investigations. The fort will continue to offer a family-friendly tour each night at 7:00 p.m. This is a walking tour of the grounds around the Fort William Henry Hotel and Conference […]Continue Reading »
Tag: haunted
Here is a report from John Tobin, one of the organizers of the "Preserving the Haunt – Para History Con" which took place at the Fort William Henry Hotel and Conference Center on April 26 and 27, 2013. The event was sponsored by Glory Haunt Hounds and Beyond Ghosts. Overall the event was a success. The […]Continue Reading »
On Friday, April 26th and Saturday, April 27th, 2013, Upstate NY paranormal groups Glory Haunt Hounds and Beyond Ghosts will be hosting a celebrity filled weekend for Paranormal and Historical enthusiast alike. "Preserving the Haunt" Para-History Con will be held at Fort William Henry Hotel and Conference Center. Celebrities who will be attending are John Zaffis […]Continue Reading »


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GraphicRiver Radio Chat 8436119
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GraphicRiver Radio Chat 8436119
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Radio \ Talk \ Station \ Online \ Podcast \ Recording \ Sound \ Show \ Studio
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Healthy Cook; Modern Housing; Healing Clinic; Security Print ; Digital Writer; Racing Pro; Compass Core.
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The Young and the Elderly at Risk
Edited by: Ioana Salagean, Catalina Lomos, Anne Hartung
The current retrenchment of the welfare states is buffering the growing demographic and economic pressures in European countries at the expense of the young and the elderly. However, both investment in the young, which determines a society's future, and providing public support for the elderly, the most deserving needy group, are seen as musts. This book encompasses a selection of empirical studies reflecting on when and why the young and the elderly are at risk in several (mostly Western) European countries. The book's contributions examine the educational achievement and the labor market entry of youths, particularly those who have a migration background; the poverty risk experienced by the elderly, especially if they are also immigrants and/or women; the pension outcomes of former cross-border workers; the simulated consequences of a recent pension benefit reform, as well as those of a potential reform, including financial assets and housing wealth in old-age income protection; and finally, the extent (and possible erosion) of the support for government providing child-care and protecting the elderly. The book is dedicated to the memory of KU Leuven's Professor Jos Berghman (1949-2014). *** Empirical research, as it is presented in this book's contributions, can add much to our understanding of how this social contract, and by extension also others, like the social contracts between the active and inactive, the healthy and the sick, and the rich and the poor, can be sustained, economically, politically, as well as socially. -- Wim van Oorschot, Professor of Social Policy, KU Leuven (Series: Social Europe - Vol. 34) [Subject: European Law, Social Welfare Law, Social Policy, Politics]
Publication Date: 12/23/2015
Format: Paper


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Guardiola hails progress as five-star City put unhappy Hammers to sword
West Ham United 0-5 Manchester City
Pep Guardiola hopes Manchester City's 5-0 of thrashing West Ham in the FA Cup third round will give his players more self-belief.
Yaya Toure's penalty, an own goal from Havard Nordtveit and further strikes from David Silva, Sergio Aguero and John Stones earned City a resounding win at London Stadium.
Boss Guardiola said: "Finally, we were able to keep the ball more than the last games, where it was almost impossible, so we played much, much better in that sense.
"We create more chances when that happens and we concede few and of course it helps us a lot.
"Before the penalty we had three or four clear chances and after the second and third goal it was easy for the second half," he insisted.
"Always it is important to win away, it's not easy in West Ham in the cup.
"Hopefully it can help us to make our players believers that they are good enough to play every game. They have to believe they are able to compete wherever."
The penalty decision which opened the floodgates may have been debatable, but the result was not and vindicated Guardiola's decision to field a strong line-up.
The City boss has endured a strange week after a defeat at Liverpool and a series of tetchy post-match interviews following the win at Burnley.
But rather than weaken his team for his first FA Cup tie, the Spaniard proved he is taking the competition seriously by recalling Silva and Aguero.
West Ham, by contrast, started with their star man Dimitri Payet on the bench and immediately found themselves under the cosh.
Silva's early cut-back found Pablo Zabaleta, whose goalbound shot was bravely blocked by Winston Reid, before Gael Clichy set up Silva only for his effort to be kept out by Adrian at the near post.
Raheem Sterling was then denied by a superbly timed Reid tackle and from the resulting corner Adrian tipped Aguero's volley over.
The pressure was always going to tell, though, and in the 32nd minute Zabaleta tumbled to the floor under Angelo Ogbonna's challenge.
There was only minimal contact but referee Michael Oliver quickly pointed to the spot and Toure stepped up to lash the penalty past Adrian.
West Ham may have felt hard done by but there was no justification for the collapse which followed.
They did almost hit back immediately when Michail Antonio forced a save out of Willy Caballero, but Sofiane Feghouli was unable to tuck the rebound into an unguarded net.
That proved a false dawn as City doubled their lead in the 41st minute, Sterling arriving at the far post and forcing an own goal from Nordtveit as he tried desperately to clear Bacary Sagna's cross.
Suddenly it was all too easy for City as two minutes later Sterling took his turn to race down West Ham's unmanned left-hand side.
The England international laid the ball across goal to Silva, who from six yards out had time to take a touch, steady himself, and probably do a crossword had he felt like it, before slotting in the third.
Five minutes after the interval it was four, Toure rifling a shot through a crowded area and Aguero diverting the ball into the net.
A chastening evening for West Ham got even worse six minutes from full-time when the visitors' £47.5m man Stones headed in Nolito's corner, as Guardiola served notice that City might just fancy a crack at lifting the famous trophy in May.
Belfast Telegraph


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Individual Match Highlights: Lee Chung-Yong (Bolton) vs Birmingham
Although still a relative unknown in Blighty, in his native South Korea he is a superstar.
On Saturday, Bolton's new 21-year-old South Korean signing Lee Chung-Yong netted his first League goal since his £2.2m summer arrival from FC Seoul to earn the Trotters a valuable three points in their battle to stay in the Premier League. A free-kick from Matty Taylor cannoned off the post before rebounding to the South Korean to put the visitors back in front minutes before the fulltime whistle.
Lee's winner, with arrived four minutes from time, allowed the South Korean to atone for a dreadful miss and for giving the ball away in the build-up to Kevin Phillips's goal which appeared to have earned the Midlanders a point.
It had been an eventful half-hour for the substitute midfielder who had also squandered a chance to put his team two goals ahead when he rounded Hart and rolled his shot wide. Individual match highlights of Lee Chung-Yong's performance against Birmingham can be seen here.
As a bonus, we also have a video capturing Cristiano Ronaldo's performance for Real Madrid in the 3-nil victory over Tenerife from Saturday night.
Ronaldo's amazing goalscoring run finally came to against the minnows but that wasn't for a want of trying. Always a threat, CR9 was frustrated by Tenerife keeper Aragonenses who did particularly well to deny the forward after he skipped past several challenges and fired a bullet on target with the shot-stopper did well to turn aside.
Individual match highlights of Cristiano Ronaldo's performance against Tenerife can be seen here, while a cute flick from the trickster can be seen here.


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Trump Got Compared To Hitler By A Republican Senator Who Just Left The Party
The Republican exodus brought on by Trump's victory has begun! Even some from within the GOP are actively calling Trump's actions against a federal court judge racist. The most famous being Paul Ryan; even though Ryan has refused to actually rescind his support. In a typical 6-year-old "Nuh uh you are"Read More


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Author has written 1 story for Harry Potter.
To any and all who were fans of the stories True Darkness Rising and Shadows Of Madness.
I'm sorry that they're no longer available. I've recently had a very painful case of writers block and decided to take a look through them... I wasn't very happy with what I saw. By no means are they bad but there were to many mistakes, to many things I want fix and change. I'll be redoing them and put them back once I've accomplished a bit more of what I had envisioned them to be.
Thank you for the kind words I've received about the stories and I hope when I upload the improved versions those who liked them will follow me again.
Yours truly, Ravenfeathers823


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You are here
DIRT: Detritus Input and Removal Treatments
Although organic matter is a critical constituent of soils that provides structure and influences many properties and biogeochemical processes, the fundamental processes controlling organic matter incorporation and dynamics in soils are poorly understood. In the DIRT experiment we are investigating biological and physical controls on soil organic matter storage. Since 1990 we have manipulated above- and belowground litter inputs to soils in a mixed hardwood stand.
Treatments include:
- 2x litter
- no litter
- no roots (trenched)
- no roots-no litter
- OA-less (one time replacement of organic and A horizons with B horizon soil)
The OA-less treatment was implemented to track soil recovery after impoverishment.
We have routinely measured key processes (soil respiration and nutrient leaching), surveyed site resources, and characterized baseline soil properties. Potentially mineralizable C and N (long-term laboratory incubations) and depth profiles for 13C and 15N contents were determined for all soils collected at the start of the study. We are collaborating with other LTER sites to establish similar experiments along climatic and edaphic gradients in North America.
This project began in 1990 and is supported by the Harvard Forest Long-Term Ecological Research program.


text: Human Rights Observatory Analysts Debate Way forward For South Sudan South Sudan has been independent for nearly three years. The Sudan People's Liberation Movement, which had fought a decades-long war from the Republic of Sudan, assumed control of the government and began state-building. By last July, ruling party rifts were playing out in public. President Salva Kiir sacked a cabinet of dissenting voices, including vice president Riek Machar. In December, Machar and others had complained about a lack of democracy in...Read complete article© Voice of America - Monday, February 24, 2014


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Surface PRO
The 128GB Surface Pro as well as its 64GB version has sold out at Microsoft's US online store, and checks suggest a lack of stock at both the company's retail stores.
Microsoft's Surface tablet will be priced from $499 for a 32GB version in the US and it will come out on 26 October in eight countries including the UK, the company said on Tuesday.
Microsoft Corp introduced its own line of tablet computers on Monday at a much-hyped press event in Los Angeles, marking a major strategic shift for the software giant as it struggles to compete with Apple Inc and re-invent its aging Windows franchise.


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5 Activities To Help Children With Autism Learn By Themselves
When your child is engaged and happy to participate in activities with you, learning becomes faster and easier. This ability to adapt, to constantly monitor one's surrounding and oneself, is called dynamic intelligence. Here are some activities which you can deploy to take the first steps towards building intrinsic motivation in your child.


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Switzerland and the EU have signed a major accord to automatically exchange information on the financial accounts of each other's residents. EU taxpayers will no longer be able to hide undeclared funds in Swiss banks.
After coming up to increased international pressure in recent years, Switzerland has signed up to a few exchange of information agreements, bringing an end to its long tradition of banking secrecy.
The new tax transparency agreement with the EU was signed in Brussels on 27th May 2015, by EU Tax Commissioner, Pierre Moscovici; Swiss Secretary for International Finance Matters, Jacques de Watteveille, and Latvian Finance Minister Janis Reirs (Latvia currently holding Presidency of the EU Council).
The European Council describes it as an important step in efforts to clamp down on tax fraud and evasion. It estimates that EU member states lose around €1 trillion of tax revenue each year to tax evasion.
The accord will replace the EU-Switzerland taxation of savings agreement that has been in force since 2005. Under that agreement, Swiss banks deduct a withholding tax from interest earnings of EU account holders, rather than share information automatically, thereby keeping banking secrecy intact. But those days are over.
Swiss financial institutions will start to collect the necessary client and account data from January 2017, and transmit it to the Swiss tax authorities. From 2018 this information will start to be forwarded to the tax authorities of the clients' country of residence. This will take place on an annual basis, and for every client who lives in the EU.
The tradition of banking secrecy in Switzerland is coming to an end. Peter Worthington, Senior Partner at Blevins Franks, explains what this means for you living in Mallorca. […] 'Switzerland Pledges To End Banking Secrecy' by Blevins Franks
The data to be shared includes names, addresses, tax identification numbers and dates of birth, plus the financial information relating to the account.
The accord includes provisions to limit the opportunities for people to move assets or invest in products outside the scope of the agreement in order to avoid being reported to the tax authorities.
The information to be exchanged therefore includes not only income like interest and dividends, but also account balances and proceeds from the sale of financial assets.
The European Commission is concluding negotiations for new tax transparency agreements with Andorra, Liechtenstein, Monaco, and San Marino. The Swiss deal is however considered the most significant.
On signing the accord, Mr Moscovici said: "Today's agreement heralds a new era of tax transparency and cooperation between the EU and Switzerland. It is another blow against tax evaders, and another leap towards fairer taxation in Europe."
The Federal Council immediately launched a consultation which is open for comment until 17th September this year. The agreement will then be submitted, together with a dispatch, to the Swiss Parliament for approval.
Peter Wothington, Senior Partner at Blevins Franks Mallorca writes about Spain and Offshore Assets. […] Spain and Offshore Assets by Blevins Franks
In the coming weeks, the Federal Council will adopt dispatches on the Automatic Exchange of Information Act, the Multilateral Competent Authority Agreement, and the Organisation for Economic Co-operation and Development Economic Co-operation and Development (OECD)/Council of Europe administrative assistance convention.
Switzerland has been working to improve its image on tax policy. Banking secrecy and the inability to collect unpaid revenue has strained relations with countries like the US, France and Germany. Banks like UBS and Credit Suisse had had to pay large fines and hand over data to the US authorities.
It has signed up to both the US' Foreign Account Tax Compliance Act (FATCA), and the Common Reporting Standard established by the OECD. Almost a hundred countries have signed up for that so far, taking automatic exchange of information to a whole new global level.
It has also started investigations into allegations that a Swiss subsidiary of HSBC helped clients hide money in secret bank accounts to avoid paying millions in taxes.
As the OECD has explained, automatic exchange of information "can provide timely information on non-compliance where tax has been evaded either on an investment return or the underlying capital sum, even where tax administrations have had no previous indications of non-compliance."
The cross-border tax landscape has changed considerably. It is important to be informed on developments and take professional advice to ensure your tax planning conforms to local tax law and does not result in unexpected consequences. With specialist advice from Blevins Franks, it is possible to take advantage of tax compliant opportunities to protect your assets from the various Spanish taxes.
For more information and personalised advice, contact Blevins Franks on +34 971 719 181.
To keep in touch with the latest developments in the offshore world, check out the latest news on Blevins Franks Tax & Wealth Management Specialists.
Related articles to tax for foreigners living in Spain.
Blevins Franks Mallorca specialises in giving advice for tax-driven, wealth management investments. […] Blevins Franks Tax & Wealth Management Specialists
Blevins Franks Financial Management Limited (BFFM) is authorised and regulated by the Financial Conduct Authority in the UK, reference number 179731. Where advice is provided outside the UK, via the Insurance Mediation Directive from Malta, the regulatory system differs in some respects from that of the UK. Blevins Franks Trustees Limited is authorised and regulated by the Malta Financial Services Authority for the administration of trusts and companies. Blevins Franks Tax Limited provides taxation advice; its advisers are fully qualified tax specialists. This promotion has been approved and issued by BFFM.
This article was written on the 26th of July, 2015.


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Dazzling Tiffany prong set red rhinestone clip on earrings by Juliana, DeLizza and Elster. (Also known as D&E) These earrings features a large oval red rhinestone at their center. Flanked by pink navette stones and chaton cut red and pink stones, these exceptional earring really sparkle.
These earrings have all the qualities we love in D & E jewelry...prong set stones, well soldered backs and exceptional design. The clip on earrings measure 1 1/4" x 1" and are in excellent vintage condition.
2 other shoppers have this item in their Cart or Wish List .
Vintage Jewelry, Toys, Books, Dolls and Much More
Rare books, childrens literature, dolls, vintage jewelry and more.


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Justin Jackson's book Jolt: Marketing Tactics for Programmers, Designers, Freelancers, Makers, and Entrepreneurs was a great way to start off the year.
It really does give you a Jolt of inspiration.
This book is a great resource for marketing your new product, software, idea, book, or any project that you want to promote. It teaches you to, "Be different, break out of the mold, and surprise folks".
Justin provides case studies, examples of websites and services using data that you might not known before.
One of the stories he provides is about being first to the party:
There is a first mover advantage that you get on any new platform. In the early days of Twitter, people who grabbed a foothold there were able to amass a big number of followers, even though they might not have been famous elsewhere.
I joined Twitter in May 2007 and can attest to this. Those of us that were there at the beginning ended up with much more engagement with early adopters, forged friendships, and benefited from the platform. Whale is an upcoming platform that I'm seeing the same pattern emerge as I did then.
He goes on to say:
You can set yourself up to create [luck]. Quantity is what gets you to quality. Start placing little bets; try a bunch of things. One of them might work and become big.
This is great. Validate your ideas. Try to see what works, evaluate the data, and keep iterating on what does work. That's the key. Look back at what you've done, revise, and keep pushing forward.
A few of my favorite authors' books for Kindle are on sale on Amazon right now. They were so good that I had to share.
Remember, Amazon sales end quickly so I wouldn't wait too long if anything strikes your fancy.
The 4-Hour Workweek ( expanded and updated — 91% off ) by Tim Ferriss is on sale for $1.99. | Bring automation and efficiency to your business.
Choose Yourself by James Altucher is on sale for $.99. | The world has changed. Learn how to leverage those changes.
The Choose Yourself Guide To Wealth by James Altucher is on sale for $.99. | New rules for making money based on recent changes in society.
Crush It! by Gary Vaynerchuck is on sale for $3.99 ( 82% off ) | Do what you love. No excuses.
I was recently recommended to give the book Strengths Finder 2.0 a quick read. It is very much a short read but worth it indeed. The book shows you the importance of building your strengths and minimizing your weaknesses.
Recommended to get it on Kindle format as your online code is emailed immediately.
It includes an online code to take Gallup's StrengthsFinder quiz, which reveals your top 5 strengths. I won't go into all of mine, but my first one was incredible: Ideation.
There are many books that I've read. My library continues to grow – even with books that I haven't read yet. My favorites, the best that really speak to me, are few in all honesty. Lately, I've been reading a string of books that I wish I would have had 10 years ago ( Linchpin, Design Is A Job, etc.. ). Steal Like An Artist is now on that list.
What the Plus!, by Guy Kawasaki, is a tour guide and user's manual for Google's newest product: Google+.
This reference is great if you are just starting out with Google+ and want an extensive map on making the most of this new platform. And for the "experienced" early adopters who jumped on board in the beginning, you may be surprised to learn a few new tricks yourself.
Seriously. If only I would have had this book 10 years ago.
Jeffrey Zeldman, thank you for pushing Mike Monteiro to write. The design world will be a better place for it.
Most people are taught money management skills when they are younger. They either learn by observing, experience, or from their peers when they get older. And then some of us need a book…
I Will Teach You to be Rich, is a great irreverent style book. It is geared towards young people ages 20 to 30. This no nonsense approach teaches you that there is more to saving money then breaking your habit of lattes.
One of my most favorite books ever. Repeatedly, I try to consume it as much as possible: hardcover, Kindle edition and audiobook. This book has changed my life.
Do one thing at a time. Do it well. – Leo Babauta
The Power of Less is a testament to itself. Author Leo Babauta hits the right points and delivers in a clear concise way. No clutter or fluff — just what you need.
In January 2011, I read The 100 Thing Challenge by Dave Bruno. I highly recommend reading the book if you are looking to simplify your life. Many of us have different reasons for doing so.
Personally, I had been toying around with the idea of scaling back my possessions to only the necessary. And then I found this book.


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Accueil | Case studies | Office Depot
Office Depot
Office Depot offers the largest selection of office products in the world; they are leaders in providing their customers with solutions that allow their business to run smoother.
metapeople, part of NetBooster Group has been successfully working with Office Deport on their digital marketing strategy since 2012.
- Office Depots' main goal was to gain new customers and to increase online sales across their 10 key regions internationally, includng Germany, Switzerland, Belgium, UK, Italy and Spain
- Additional goals included the reduction of the costs-per-order as well as the improved use of landing page
- The first step of the solution was for the metapeople team to optimise Office Depot's keywords, ad copy and landing pages
- Account re-structuring was carried out in the ad group as well as at campaign level
- The ad groups were made as specific as possible and a variety of new and beta Google features were used to create efficiencies
- Fully transparent, real-time tracking was implemented across all markets and channels
Bounce Rate
Optimisation efforts resulted in 31% decrease in bounce rate
Efficient scaling of the campaigns led to an impressive increase in conversions
CPO was halved from Q2 to Q3


text: Hurton's Armed guard discovers the astronauts spying on the fishing camp and captures them. Retrieved from "http://planetoftheapes.wikia.com/wiki/Armed_guard?oldid=37357" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Gorillas Living Characters TV Characters Gorilla Guards Characters Male Characters Apes Living Apes Add category Cancel Save


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EZDoctor Helps Over 750,000 Patients Find New Doctors
Dec 31, 2015, 08:35 ET from EZDoctor
FORT LAUDERDALE, Fla., Dec. 31, 2015 /PRNewswire/ -- During this open enrollment season, patients looking for a new primary care doctor but don't know where or how to start their research can now get help directly from the experts. EZDoctor, an innovative healthcare technology company just announced that it will be assisting over 750,000 healthcare consumers this open enrollment season in the process of finding a doctor.
Logo - http://photos.prnewswire.com/prnh/20151230/318493LOGO
This year's open registration period has brought with it a very important change; 12 of the 23 Consumer Operated and Oriented Plans (CO-OPs) will no longer offer health insurance coverage through The Patient Protection and Affordable Care Act, also known as Obamacare. This change has impacted over 750,000 consumers who enrolled in those co-op health plans and now at the end of the year, will lose their health insurance coverage.
Patients now need to find new health insurance plans through the marketplace and select new doctors. According to recent news, 11 more co-ops are possibly in danger of going under.
EZDoctor.com, the fast-growing hub that connects patients with doctors and has developed the most comprehensive physician background checks available, will be assisting those patients across the country in this overwhelming process.
Not only can patients find great doctors and schedule appointments but also research potential physicians before choosing them or booking appointments, making the whole process even easier, more convenient and transparent.
With EZDoctor's physician background checks, called EZDoctor Reports, healthcare consumers are able to view a doctor's background information, screen them and decide if a doctor would be a good fit based on price, experience, education/training and disciplinary/malpractice history. Whether searching for a primary care doctor, pediatrician or specialist, or if it's an HMO or PPO health plan, EZDoctor's tools make the process of finding a new healthcare provider quick, easy and transparent.
As part of EZDoctor's goal of making the process of choosing a doctor seamless and transparent, its team is working to give consumers access to the most comprehensive reports available on doctors today. Patients should have all the information they need to make well-informed and educated healthcare decisions.
Michelle Beltre
Marketing Communications Specialist
Ext. 108
SOURCE EZDoctor


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Welcome | Sign In or Contact Us
Sump Pumps Direct Announces Addition of Superior Pump Sump Pumps
Online Superstore Continues To Expand Sump Pump Offering
Receive Weekly How-To Tips & Specials
Sump Pumps Direct announces the new superior pump sump pumps and the 2010 superior pump sump pumps. The product experts chose the new superior pump sump pump based on sales history and customer reviews. Read recommendations on the new superior pump sewage pumps from the experts who work with them every day.


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The Xilinx Spartan(TM)-3A family of I/O-optimized field programmable gate arrays (FPGAs) is an extension of its low-cost, high volume Spartan-3 Generation. The Spartan-3A platform provides a cost-reduced solution for applications where I/O count and capabilities matter more than logic density. With support for the industry's widest range of I/O standards (26) and unique power, configuration capabilities and anti-cloning security advantages, Spartan-3A FPGAs provide a flexible and cost-saving solution to new high-volume applications within consumer and industrial segments, such as display panel interfaces, video/tuner board interfaces and video switching.
Built on 90nm technology, the new platform consists of five devices offering up to 1.4 million system gates and 502 I/Os. Offering the lowest cost per I/O in the industry, the new family also features significant advances in power management, device configuration and design security. The Spartan-3A platform integrates an impressive array of innovative features and technologies including:
- Widest I/O Standards Support: First TMDS-compliant FPGAs enabling DVI and HDMI support for consumer video applications, and the only FPGAs compliant to 26 popular single-ended and differential signaling standards. Source-synchronous interfacing technology cost-optimized to ensure best design margins. Pre-engineered solutions for PCI, PCI Express, USB, Firewire, CAN and SPI among others, as well as support for all popular low-cost DDR and DDR2 memory interfaces up to 333 Mbps.
- Only Dual-Power Management Solution: Suspend mode with over 40 percent static power reduction, fast wake-up time of less than 100 microseconds and system-level synchronization across time domains. Hibernate mode provides up to 99 percent reduction in static power and wake-up time of less than 100 milliseconds.
- Comprehensive Configuration Capabilities: Source-synchronous interfacing technology cost-optimized to ensure best design margins. Enhanced multi-boot capability with integrated watchdog supervision for guaranteed 'golden' configuration.
- Robust Anti-Cloning Security Feature: The Spartan-3A devices are the industry's first FPGAs to offer a unique DeviceDNA serial number. This permanent ID code safeguards hardware and software IP, providing designers with the flexibility to implement custom algorithms that enable authentication, deter cloning, and control IP activation.
Pricing and Availability
Customers can immediately begin designing systems with Spartan-3A FPGAs using the ISE(TM) design tool suite, Spartan-3 Generation library of application-specific IP, and new Spartan-3A Starter Kit. Engineering samples are shipping now for the XC3S700A and XC3S1400A devices with the XC3S50A, XC3S200A and XC3S400A devices to follow in the first quarter of 2007. At customer production timeframes in 2007, the 3S700A device will list for $11.95 and the 3S1400A device at $16.95, both in 250K unit volumes.


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Matthew and Camila's Levi Looking Like a Little Man Already
Matthew and Camila's Levi Looking Like a Little Man Already
Matthew, Camila, and Levi got back to their routine in Malibu over the weekend, including jogs for Matthew and shopping trips for the whole family starring the already stylish baby. While McConaughey may not be running away with the favorite new celebrity parent of 2008 poll, he still seems so much warmer and happier now that he's growing into his role as a father. That isn't to say that Matthew and Camila aren't going to pass their free spirits onto little Levi, but MM already seems to have bigger things on his mind than trying to keep track of his flip flops.
To see more of Matthew, Levi, and Camila just


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GRI starts work on revisionJune 2011
The Global Reporting Initiative (GRI) is asking its network and the public for input as it begins work on new sustainability guidelines.
The fourth generation of the GRI's sustainability reporting guidelines is expected to expand significantly, as well updating and clarifying the present G3 guidelines, which have been in place since 2006.
GRI says the new guidelines will be a response to the growing importance of non-financial reporting as it will 'need to be standard-ready' while it enters 'a mainstreaming phase'.
Therefore, G4 will specifically aim to help companies to report better to their various stakeholders, to harmonize available frameworks and principles, and to provide more advice on the integration of financial and non-financial performance data.
The new guidelines are also being seen as a stepping stone for companies preparing an integrated report based on the framework being developed by the International Integrated Reporting Committee.
There is at present a call for sustainability reporting topics that members and the public would like included.
Already a member? click here to login


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Lady Lucy Upton's tongue may be too sharp to attract suitors, but her heart is good, and when her painfully shy friend Cassandra needs help, she devises a brilliant scheme to help her discourage an unwanted suitor, the Duke of Claringdon. Lucy will hide behind the hedgerow and tell Cass just what to say to discourage the Duke of Claringdon...but it turns out that he's made of sterner stuff than either of them anticipated. And Lucy is shocked to discover that tangling with the tenacious man is the most fun she's had in ages. Lord Derek Hunt made a promise to his dying friend to marry the demure Cassandra, and for a man who wants nothing more than peace and quiet after the horrors of war, she'll make the perfect bride. If only the impudent Miss Upton will let him court the girl! Doing battle is the last thing on his mind, but bantering with Lucy behind the bushes is too tempting to resist. And the spoils of this war just may be true love.
©2014 Valerie Bowman (P)2014 Tantor
"Could not deal with the reader"
I made it through about 20 minutes of the book before giving up. The narrator reminded me too much of Moaning Myrtle from Harry Potter.
"Didn't enjoy the narrator"
I'm a huge fan of Valerie Bowman and have read or listened to all of her books. This is a great story but for me personally it was ruined by the narrator. While she is charming and well spoken her cadence was to fast and her voice made the characters sound very young and child like. I couldn't get into the story and had to stop half way through and get the book to read. I enjoyed the plot and story very much, just didn't enjoy the narrator.
"Great group of friends and sweet romances"
Lucy and Derrick provide fun, sharp, witty banter. Cass and Jane are wonderful friends. Garrett was a great cousin and friend.
Alison does the job of British and period diction with both males and females.
"Loved this book!"
I've listened to all three books in this series, and I like this one the most. I enjoyed the bantering between the hero and the heroine. Their banters were so much fun to listen to that I couldn't help but smile several times. Both characters are strong-willed, clever, witty, and loyal. They are perfect for each other! The love scenes were pretty hot and steamy.
I felt that some parts toward the end of the book kind of dragged a little.
I really liked the narrator. I wish she had narrated the final book (Book 3) in this series. However, I didn't like that she made Lucy (the heroine in this book) sound a little older in Book 2. It would be a good idea to read/listen to the books in order, because Lucy and her friends appear in each book and a few references are made from each preceding book.
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If you find this review inappropriate and think it should be removed from our site, let us know. This report will be reviewed by Audible and we will take appropriate action.


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Traveling Luck for Briscoes Mill (historical), Mississippi, United States
Where is Briscoes Mill (historical)?What's around Briscoes Mill (historical)?
Wikipedia near Briscoes Mill (historical)
Where to stay near Briscoes Mill (historical)
The timezone in Briscoes Mill (historical) is America/Rankin_Inlet
Sunrise at 06:34 and Sunset at 16:53. It's light
Latitude. 33.2631°, Longitude. -89.6208° , Elevation. 100m
Weather near Briscoes Mill (historical); Report from Greenwood, Greenwood-LeFlore Airport, MS 64.3km away
Weather :
Temperature: 18°C / 64°F
Wind: 0km/h North
Cloud: Few at 12000ft
Weather :
Temperature: 18°C / 64°F
Wind: 0km/h North
Cloud: Few at 12000ft
Satellite map around Briscoes Mill (historical)
Loading map of Briscoes Mill (historical) and it's surroudings
Geographic features & Photographs around Briscoes Mill (historical), in Mississippi, United States
- stream;
- a body of running water moving to a lower level in a channel on land.
- Cross Branch (2km)
- Baxter Creek (3.4km)
- Bagasha Creek (4.5km)
- Panther Creek (5.3km)
- Scoobachita Creek (5.6km)
- Redden Branch (6.1km)
- Taylor Creek (7.3km)
- Braswell Branch (9.1km)
- Little Zilpha Creek (9.4km)
- Scott Branch (9.4km)
- Little Horse Creek (9.6km)
- Mayo Branch (10km)
- Randle Creek (10.4km)
- Hays Creek (10.7km)
- cemetery;
- a burial place or ground.
- Providence Cemetery (3.4km)
- New Salem Cemetery (4.5km)
- Oak Grove Cemetery (6.4km)
- Shiloh Cemetery (7.9km)
- Old Plank Cemetery (8.1km)
- Grantham Cemetery (8.9km)
- Newhope Cemetery (8.9km)
- Friendship Cemetery (9.3km)
- Joe Walker Cemetery (9.3km)
- Daniel Cemetery (9.6km)
- Old Friendship Cemetery (9.7km)
- Sand Hill Cemetery (9.7km)
- Old Friendship Cemetery (10km)
- church;
- a building for public Christian worship.
- Providence Church (3.4km)
- New Salem Baptist Church (4.5km)
- Hebron Church (5.9km)
- Oak Grove Church (6.4km)
- Shiloh Church (7.9km)
- Old Plank Church (8.2km)
- Friendship Church (9.7km)
- Sandhill Church (9.7km)
- Bethany Church (9.9km)
- populated place;
- a city, town, village, or other agglomeration of buildings where people live and work.
- North Center (historical) (3.8km)
- Chita (historical) (5.8km)
- Carmack (5.9km)
- Friendship (historical) (9.5km)
- Sand Hill (historical) (9.7km)
- Auris (historical) (9.9km)
- school;
- building(s) where instruction in one or more branches of knowledge takes place.
- North Center School (historical) (3.5km)
- New Salem School (historical) (4.5km)
- Carmack Elementary School (5.9km)
- New Hope School (historical) (8.9km)
- Friendship School (historical) (9.4km)
- Macedonia School (historical) (10.8km)
Accommodation around Briscoes Mill (historical)
- Local Feature;
- A Nearby feature worthy of being marked on a map..
- Briscoes Mill (historical) (0km)
- Mitchells Mill (historical) (4.2km)
- Wesley Chapel (5.9km)
- Denmans Ferry (historical) (10.4km)
- Ferguson Box (historical) (10.6km)
- Kirkwood Ferry (historical) (10.7km)
- dam;
- a barrier constructed across a stream to impound water.
- Wade Lake Dam (2.9km)
- Mississippi No Name 1 Dam (3.6km)
- Armstrong Lake Dam (7.3km)
- Dan Oak Dam (10.7km)
- post office;
- a public building in which mail is received, sorted and distributed.
- Zilpha Post Office (historical) (0.1km)
- lake;
- a large inland body of standing water.
- Denman Lake (9.7km)
- mountain;
- an elevation standing high above the surrounding area with small summit area, steep slopes and local relief of 300m or more.
- Arnold Mountain (7.7km)
Wikipedia entries close to
Briscoes Mill (historical)
- Vaiden, Mississippi city city o
- West, Mississippi city city o
- Kilmichael, Mississippi city city o
- French Camp, Mississippi city city o
- Ethel, Mississippi city city o
- Rainwater Observatory and Planetarium science
- Kosciusko, Mississippi city city o
- McCool, Mississippi city city o
- Winona, Mississippi city city o
- Sallis, Mississippi city city o
- Durant, Mississippi city city o
- Weir, Mississippi city city o
- Holmes County State Park park
- Carrollton, Mississippi city city o
- North Carrollton, Mississippi city city o
- Duck Hill, Mississippi city city o
Airports close to Briscoes Mill (historical)
Greenwood leflore(GWO), Greenwood, Usa (64.3km)
Jackson international(JAN), Jackson, Usa (146.7km)
Columbus afb(CBM), Colombus, Usa (150.2km)
Meridian nas(NMM), Meridian, Usa (163.6km)


text:
Bad weather
For the latest information about Highways, gritting and school closures please visit:
Waste collections
In the event of snow and ice disrupting waste and recycling collection services the following guidelines will generally apply:
|Type of collection missed
|Action to take
Refuse Collections (in the event of a missed collection; you will be permitted to put out extra waste in refuse sacks)
Present waste at the kerbside by 7.30am the next morning and leave it out for up to a week
Recycling collections (blue bag, green box or food waste)
Present recycling at the kerbside by 7.30am the next morning and leave containers out for up to three working days
Garden Waste collections Please be aware that this service may be suspended so that the vehicles can be used to collect any outstanding refuse (as per the terms and conditions of the garden waste license detailed at time of purchase)
Take wheeled bin back in and present it on the next scheduled collection day
Updates will be available on:
- this page, the Council's Facebook page and Twitter
- the waste and recycling automated telephone line, 01594 812666
- Local radio stations.
We will try to maintain services as long as it is safe to do so, we assess conditions and take into account:
- health and safety of the crews and the public,
- being able to manoeuvre vehicles safely,
- the accessibility of the depot, disposal sites and properties due for collection each day, and;
- the weather forecast.
If collections are missed, a catch up plan will be put in place so that crews will return as soon as conditions improve. The crews will work late and on Saturdays to implement the catch up plan, as required.
Missed refuse collections will be given priority.


text:
Ivar Vivahitharayal...? Trailer (2009)
12 June 2009 Drama, Comedy, Family
Vivek does his MBA degree from Pondicherry University. Even while as a student, the main ambition of Vivek is to get married as soon as he is back home after his studies. Vivek has a circle of very close friends of four, with whom he discusses everything. A phone-in programme encounter with Kavya aka Tinku (Bhama), a radio jockey, and Vivek, results in loss of Kavya's job. Some strange circumstances lead to the marriage of Vivek and Kavya, without knowing each other that they are the same people who clashed on the FM radio channel. Vivek expects Kavya to be an orthodox and simple wife of his dreams and Kavya turns out to be otherwise.
Watch the official Ivar Vivahitharayal...? 2009 trailer in HD below or find more Ivar Vivahitharayal...? videos on Vidimovie.


text:
GraphicRiver Holiday Blue Background with Tree Branches 3718158
Stock Vector \ Conceptual \ Seasons \ Holidays \ New Year
Background \ Vector \ Branches \ Illustration \ Editable \ Winter \ Objects
Stock Vector - Holiday Blue Background with Tree Branches 3718158 by almoond @ GraphicRiver
Stock Vector \ Conceptual \ Seasons \ Holidays \ New Year
Holiday blue background with tree branches. Vector illustration. Fully editable, vector objects separated and grouped, no blends, gradient mesh used
Layered: No; Graphics Files Included: Vector EPS; Minimum Adobe CS Version: CS.
Drop of Water Background ; Background with Butterflies; Three Saint Patricks Day Banners; Spring Background ; Nature Background; Spring Background ; Three Happy Easter Banners.
Keywords: background, banner, blue, branches, card, cold, color, concept, design, frame, gift, green, happy, ice, icon, merry, new, note, ornate, party, season, shape, single, snow, star, symbol, tree, winter, xmas, year.


text: So I decided to wipe the phone couple days after 2.2 update. I was experiencing severe lag time while typing. Also zooming (double-tap) in browser wouldn't work. Anyway, I cant get appbrain to sync. I installed appbrain on phone and have signed in with same account but none of my apps show up just the pre-installed ones on the phone. When I look on web all my apps still show up in My Apps. Thanks


text:
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Spotted guinea fowl feathers are a popular craft feather. When dyeing the white spots take the dye creating a unique look for an inexpensive craft feather. Dyed guinea feathers are sold by the half ounce packages. Dyed colors include blue, red, turquoise, green, gold, orange, lavender, pink and purple.
~Call if you would like a quote per pound!


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Fire Lite 411 Alarm Manual Featured
fire-lite-411-alarm-manual.pdf (285 views)
Fire Lite 411 fire alarm installation, operation, programming and user manual guide. Learn the procedure for installing, maintaining, servicing and inspecting your fire system to current federal, state and local code. Download a Fire Lite 411 alarm manual online.
Images (1)


text:
Ceramic Baby Bottle Bank by Cambium Designs 1986
Sold for:
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- Sold Date:
- Source: eBay
Welcome To My Dolly Dearest on eBay.....an experience in collecting!
1986 Baby Bottle Bank by Cambium Designs
Baby Bottle Bank designed and manufactured by Cambium Designs and first offered in 1986. It measures 14" tall, is made of ceramic, and is in the shape of a large baby bottle. It features a teddy bear and toy design with places to put the time the baby was born, date of birth, weight, and the name of the baby. When a coin is put into the slot, the tune "It's A Small World" plays. It has a removeable rubber stopper in the bottom and is marked "CAMBIUM DESIGN/@1986/BABY BOTTLE BANK". It is preowned and in beautiful condition.
Shipping for this item is $7.50 in the lower 48 states and includes tracking.
(International Shipping Slightly Higher)
I do not give shipping quotes to international buyers since I charge only actual shipping. So if you don't want to pay actual shipping, please do not purchase my items. I only ship Priority or Express mail Internationally. I DO NOT ship 1st class mail.
Import duties, taxes, and charges are not included in the item price or shipping charges. These charges are the buyer's responsibility. Please check with your country's customs office to determine what these additional costs will be prior to purchase.... read more


text:
Thanks for the transcript!
Written on February 8, 2015
Hey my friends, Robert here…
A few days ago, I posted a video blog that I made about overcoming fears. Below is the transcription of that video. I hope you find the top tips useful in overcoming your fears.
I Just wanted to do a quick video with today's topic "Overcoming fear'. I'm gonna talk about 3 secrets to overcoming fear and it's not actually the easiest thing you can do. Right now I'm actually in my backyard and It's pretty beautiful.
The first part is you need to acknowledge it. See, if you don't acknowledge your fear it's gonna be hard to overcome it. You don't even face it and it's important that if you don't acknowledge it, then what happens is you don't know what you wanna get over.
(Robert walking along) Alright now I'm just taking a walk with my dogs so if I stop or call one of their names in the meantime I just want to make sure that they don't go too far, don't mind me.
But anyway, the first step to overcoming fear is acknowledging it. You need to acknowledge what you're afraid of. A lot of people don't wanna face what they're afraid of and they're scared to even mention it, they're scared to even think about it, they're scared to even just have it in their mind. And the first thing that you need to do – you need to acknowledge it — making sure you're aware of what you're afraid of. If it's doing a video — acknowledging "Okay I am afraid of doing a video." Or being onstage – which, is the number one thing that people fear, right? "I have a fear of being onstage." Whatever it may be, you have to acknowledge it first to move forward because without acknowledging it, you're not letting yourself know what you're afraid of. And sometimes, you don't even know what you fear, it's just a feeling you have and you're like, "Well I don't wanna do that. I don't wanna, you know, I'm scared, I don't know what's gonna happen." And all that other stuff. We're gonna get into that.
But the next thing you wanna do after acknowledging it, is thinking about what the WORST thing that can happen if it goes wrong. Let's say you're doing a video, right and you know, like I'm doing a video right now for you folks. Let's say I mess up or something, or what's the worst that can happen? Will I mess up how I speak or I don't get my thoughts out properly or I make a fool out of myself — whatever it may be, think of the worst thing that could happen to doing whatever you're afraid of. If it's being onstage — Oh well I could make myself look foolish, or I could look funny, or I'm gonna mess up. Think of the worst thing that can happen and accept it. You know, what is the worst thing that can happen? If you get down to it, the worst thing that can happen is if you mess up — that's it! You know, what else is there? What else is there that can go wrong? I mean there's a probably a million things but the bottom line is: You did it! You overcame your fear and you just have to acknowledge the worst things that can happen, and being able to accept it like, "Okay, that's the worst thing that can happen — that's not really a big deal."
Just think about that. What's the worst thing that could happen? And when you really process that, when you think about it — there's not really that bad of things! I mean, you're still alive, you're still breathing, you still have your hearing, you still have your sight. You know, unless you're afraid of doing something that is life threatening, you're still taking a big chance. But if you're talking about like working online and our niche here, making videos – that's a fear that you have to overcome and by doing that – first, you have to acknowledge it and second, you gotta think what's the worst thing that could happen. And when you think about it, the worst that could happen is really not that bad at all! Like I said, you do a video – you mess up. Or you're writing a blogpost, maybe you don't get the words out properly, or the blogpost – you don't like it. Whatever it may be, that's the worst that could happen. It's nothing close to life threatening or any of that stuff so just DO IT, okay?
And the third part to this is: think about what's the BEST thing that can happen. You see our fears limit us so much in life — if we knew what our fear is costing us we would overcome them so much faster. Because if you really think about what the best thing that can happen to your life if you overcome these fears, you'll realize that you missed out on a lot already! And you'll overcome your fear right now! Why? Because think about it – what's the best thing that could happen? Well are you living the life that you wanna live? Are you driving the car that you wanna drive? And listen, it's not only about that but, are you helping other people to overcome their fears? You see, what happens is when you overcome your fear, you're helping other people to overcome their fears. Because, what helps me to overcome my fears is seeing other people being able to do it! And I'm like, "Sure if they can do it, so can I!"
I remember my wife and I, we went to a park. And I have a little fear for heights. And what helps me to overcome that is trusting the roller coaster. And I know that sounds weird but once you trust the roller coaster and you don't feel that it's gonna unlock and the seat belt's gonna come off and you're gonna fall out — even though those thoughts come to our mind, once you trust the roller coaster, it's more fun than fear. So what you wanna do is change the fear into fun. Make it kinda creative. Once you trust yourself…once you trust the roller coaster then you'll be able to prosper. That's something that I saw. Another thing was, I see these kids in the roller coasters that I'm like, "Wow! You know these roller coaster's like a thousand feet high." You know and stuff like that. And I see these little kids on it and I'm like, "Man! I'm such a wuzzy!" You know, when I see that, I'm like, "If they can do it…so can I!"
But anyway, so I see these little kids on the roller coaster and if they can do it, so can I! So listen, you gotta think about what's the best thing that can happen? What are your fears costing you right now? If you're afraid of doing videos – well your fear is costing you being able to show people, show other people the value that you have. It's costing you, you know, being able to express yourself on video and feel good about it.
Listen, whatever it is — your fear, it doesn't matter. Follow these three steps and you will be able to overcome them.
First, you have to acknowledge what you're afraid of. If you're afraid of going on roller coasters — acknowledge it. You know…I'm afraid of roller coasters, or the height, or whatever it may be.
Second, think of what's the worst thing that can happen and kinda being able to accept it. Now, okay I know it's a little tricky with my fear of roller coasters before because…what's the worst thing that can happen? Well, I can die, and something can go wrong and that's it. But listen, if you put that aside, and you're just like, "Let me enjoy my life. Let me enjoy my time while I'm on the roller coaster. Let me trust it. Let me trust these heights. Let me trust being able to do something else or trying something new in my business or writing that blog post or putting out that video I've been thinking about, putting about I've been having yet." Whatever it may be, there's so many examples that you could think of that people fear. But think of what's the worst thing that can happen and accept it. Kinda being able to accept it will help you to overcome it.
And then the last step you wanna think of: What is the best thing that can happen from it? See, listen, I've been able to live here in Florida. Why? Because I overcame fears that I had. I know that kinda sound weird but the fears I overcame was maybe, you know….doing videos or trying this internet thing out. See I'm new to the industry. I've only been online for about a year or so. And there were a lot of things where I could've been like, "I'm not gonna do this because I'm afraid of this. Or, I'm not gonna allow this to happen because you know, or it can't happen because I'm afraid to do it or try it." So think of the best things that can happen.
I mean it's beautiful here and it's not only about me. But you know, this only just a yard. I'm gonna bring you guys to my backyard. I'm walking towards my house now. This is just like a big yard that they have in the back like a little reservoir or whatever they call it or a little river…in case it rains, it can hold the water and stuff like that. But it's pretty cool.
Guys, when you overcome your fears, the possibilities in life is limitless. There is nothing that you can't do in life. And once you do it, you're gonna feel so good. You're gonna feel so good, I'm tellin' you. Just overcome that fear. Do that video. Put out that blog that you wanted to put out. Whatever it is – go on that roller coaster today and just face your fear. You're gonna feel good about it.You're gonna have a little bit of that fear in there, but it's okay because once you overcome it – it's just a seed of overcoming fear. And I'm tellin' you guys, once you do that, you're life's gonna change and you'll be able to grow and experience life. And the things that life can bring you once you overcome fear, you can't even imagine and they just come to you when you overcome those fears. So everything outside of your comfort zone is exactly what's gonna bring you to success in your life. So you know, that's the bottom line.
So that's exactly what you wanna do guys. Everything that you want is outside of your comfort zone and once you come and you step out of that comfort zone, you'll be able to experience things like this. Like this is actually our backyard. You'll see here that…my wife Ally is playing some music so if you can't hear me too clear…don't worry. But we got just a beautiful backyard…nice pool back here…trees…you know, and a nice little futon…and it's pretty cool. And in the night time, at lights out, this whole pool area is really nice. Ally's doing a little partying now inside…having a little fun enjoying life.
So anyway guys, that's the beauty of working from home and overcoming your fears because, another thing really quick, maybe leaving that job…I'm not encouraging you to leave the job you have or the second job that you have. But you need to be able to trust something new or try to trust something new. Give it a chance to being able to reap the rewards from it and a lot of times we feel, "Well if I leave this job or if I do this…this can happen, that can happen." Stop all of that because that's exactly what stops us from overcoming those fears. You need to try them, you need to be a little bit of a risk taker and it's okay to be. Because you see, the rewards that come out of overcoming those fears are just beautiful and you can't even imagine how your life is gonna be unless you try them.
So guys I wanna keep this short. And overcome your fears guys. Love you guys. Appreciate you. And I'll see you on the next one. Take care guys. Have a great day today!"
one comment (read it or post one here)
Thanks for the transcript!
Copyright © 2014 Robert Mercado


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A real butcher shop. Hand cut steaks to your specifications, and the best ribs I have found in this city. A good selection of fresh fish also.
Pros: Service
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From family friendly trips with kid friendly activities to local plumbers lawyers, spas, and contractors. Judy's Book has millions of listings and reviews for the best and not so great. Whether you're looking for a specific business or just trying to discover great places we make it easy. We're a family oriented site focusing on bringing trusted reviews and recommendations to moms and others. Green and Sustainable places are becoming more relevant to Judy's Book users, have a favorite place? Write a review and tell users why it's Green!


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274 Madison Ave, Suite 304 (Btw 39 and 40th str) New York, NY 10016
If insurance is not an option, your initial visit would be $90....or $84 if pay cash A variety of payment plans are accepted. Such include cash and credit/debit cards. There will be additional charge if blood test, urine test or X-ray is done. Any tests that are necessary and could cost extra will be discussed with you by the doctor in advance.
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Dr. Fuzayloff is a competent, energetic and highly dedicated physician who is board certified in Internal Medicine. He is a Diplomat of the American Board of Internal Medicine and has built a highly respected private practice in New York City. His extensive schooling includes a degree in Biology from St. John's University in New York and a medical degree from New York College of Osteopathic Medicine, where he graduated with honors in Pathology and Nephrology.
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