text: dual booted pc windows 7 and vista. i cannot seem to enable any shell commands to my evo, i have the android sdk on my pc & when clicking on the adb exe it opens then closes, when trying to issue commands through command prompt it cannot find device or random various errors occur im trying to gain write access which i thought i already had but apparently i was mistaken. my goal is to update fresh 0.3 to newest release have tried to update though rom manger but clockwork recovery is causing issues amon ra recovery installs through rom manager but i still end up stuck in htc boot screen loop when trying to update to newest fresh any helpp would be greatly appreciated


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The inflammatory endothelial response to LPS is critical to the host's surviving a gram-negative bacterial infection. In this study we investigated whether human endothelial cells express the functional coreceptor for LPS, CD14, and most importantly whether it is glycosylphosphatidylinositol (GPI) linked. We also examined whether plasma proteins could reconstitute an LPS response in CD14-inhibited endothelium. RT-PCR- and CD14-specific MAbs demonstrated CD14 expression on primary human umbilical vein endothelial cells (HUVEC) but not passaged HUVEC. The amino acid sequence of endothelial CD14 was 99% homologous to CD14 on monocytes. Endothelium responded to relatively low levels of LPS in the absence of plasma, and this was entirely dependent on CD14. Removal of GPI-linked proteins with phosphatidylinositol-phospholipase C prevented LPS detection and subsequent protein synthesis (E-selectin expression). Endothelial CD14 was sufficient to initiate functional leukocyte recruitment, an event inhibited by blocking its LPS binding epitope and also by removing CD14 from the endothelial surface. Plasma proteins restored only ∼30% of the LPS response in CD14-inhibited endothelium. In conclusion, our results strongly support an important role for endothelial membrane CD14 in the activation of endothelium for leukocyte recruitment.
- human
- human umbilical vein endothelial cells
- inflammation
- leukocyte recruitment
- glycosylphosphatidylinositol
lps is a major outer membrane constituent of gram-negative bacteria, highly immunogenic and shed during infection. This shed LPS is used as a detection system for local infection by our immune system, leading to important inflammatory responses, including leukocyte recruitment. However, under some conditions, LPS can enter the circulation, causing a systemic inflammatory response that is detrimental to the host. The human immune system is able to detect LPS by its trimeric LPS receptor: Toll like receptor 4 (TLR-4), myeloid differentiation protein 2 (MD-2), and membrane bound CD14 (mCD14). CD14 is described as a glycosylphosphatidylinositol (GPI)-linked 55-kDa glycoprotein on monocytes (9, 26), which was first demonstrated to be an LPS receptor on human monocytes (32). Pugin et al. (21) showed that responses to gram-negative LPS on murine macrophage cell lines were inhibited with CD14 blocking antibodies. The role of CD14 as an LPS receptor was confirmed by Goyert and colleagues (10) in a seminal study demonstrating high resistance to LPS-induced shock in gene-targeted CD14 knockout mice.
Isoforms of CD14 occur only via posttranslational modifications, including addition of GPI anchor. GPI-linked mCD14 has been demonstrated to be expressed on numerous blood cells: at a high level on monocytes and macrophage subsets (32) and at a lower level on granulocytes and activated or transformed B cells (10, 14, 16, 29, 35). Other studies have suggested that mCD14 is also expressed on human corneal cells (27), gingival fibroblasts (28), and epithelial cells (6). Whether endothelium can synthesize and express CD14 remains unclear. Early studies from several groups suggest that CD14 is absent from endothelium and that proinflammatory endothelial responses to LPS require soluble CD14 (sCD14) in place of mCD14 (2, 4, 22, 23). A more recent study suggested that CD14 may be present on endothelium and that its previously reported absence was due to the use of passaged endothelial cells that have reduced CD14 expression (11).
Endothelium is the critical cell for recruitment of leukocytes into tissues. In fact, recent in vivo data underscored the essential role for endothelial (but not leukocyte) TLR-4 in the early sequestration of neutrophils into lungs (1). Since TLR-4 and CD14 are closely linked partners in the LPS response, CD14 must clearly also contribute to LPS responses in endothelium. Moreover, endothelial detection of LPS may not only be relevant to acute inflammatory responses but also to more prolonged conditions such as those associated with atherosclerosis. Indeed, the absence of TLR-4 diminished development of atherosclerosis in mice (3). However, whether CD14 is necessary for endothelial responses to LPS and whether CD14 was endothelial in origin is unclear.
In this study we hypothesized that functional GPI-anchored CD14 is present on and originates from within endothelium. Using primary human umbilical vein endothelial cells (HUVEC) as our model system, we demonstrate that CD14-specific mRNA can be detected in amounts half of that in monocytes and that the protein sequence is 99% identical to monocyte CD14. Moreover, we demonstrate that CD14 is GPI anchored to the cell surface but is lost on passaging. Last, we demonstrate that primary HUVEC can initiate a maximal proinflammatory response via membrane CD14 in response to concentrations as low as 1 ng/ml of smooth Escherichia coli LPS entirely in the absence of plasma proteins and that this results in leukocyte recruitment. However, plasma proteins reconstituted ∼30% of the LPS response in CD14-inhibited endothelium. We therefore suggest that GPI-linked CD14 is present on endothelium and that its presence is important to the endothelial response to LPS.
CD14 antibody used for ELISA and blocking studies was unconjugated clone MEM-18 monoclonal antibody supplied by Hycult Biotechnology. The isotype (IgG1) control MOPC-21 was from Sigma (St. Louis, MO). FITC-conjugated CD14 antibody (clone LeuM3) and phycoerythrin (PE)-conjugated CD18 and CD54 (intercellular adhesion molecule, ICAM-1) and their matched IgG2a isotype controls were used for the flow cytometry data and supplied by Becton Dickinson Biosciences. The E-selectin MAb clone 1.2B6 (IgG1 isotype) for the E-selectin ELISA was purchased from DAKO, and peroxidase-labeled secondary antibody was obtained from Kirkegaard and Perry Laboratories. The human monoblastic leukemic cell line U937 and the EBV negative lymphoma B cell line BJAB were generous donations from Dr. S. Robbins and Dr. J. Deans, respectively (Univ. of Calgary, Calgary, Alberta, Canada). The U937 cells were matured with 50 ng/ml of PMA, and adherent cells were collected after 2 days' incubation. Smooth E. coli O111:B4 LPS was purchased from Calbiochem.
HUVEC isolation.
HUVEC were isolated from freshly obtained umbilical cords, as previously described (20). In brief, umbilical cord veins were rinsed of blood products with warm PBS, pH 7.4, and the vein was filled with warm collagenase A (Roche Diagnostics; used at a concentration of 320 U/ml in PBS). After 20 min of incubation in warm PBS, the digest was collected into centrifuge tubes containing heat-inactivated FBS (supplied by Hyclone) to neutralize the collagenase activity, and cells were pelleted. The supernatant was discarded, and the pellet was resuspended in M199 medium supplemented with 20% FBS, l-glutamine, and an antibiotic cocktail (all supplied from GIBCO), but with no endothelial mitogen. The cells were seeded into bovine fibronectin (Biomedical Technologies)-coated T25 culture flasks and grown to confluence in a 37°C humidified incubator for 4–5 days. Cells were heavily seeded to minimize cell growth and thereby permit HUVEC to express CD14, which is otherwise lost. Consequently, only first passaged HUVEC were used for all experiments.
Passaged HUVEC.
Confluent HUVEC were removed from primary fibronectin-coated culture flasks by trypsinizing (0.05% Trypsin-EDTA; GIBCO) for 30–100 s followed by the addition of M199 medium containing 10% FBS. The cells were washed, and the pellet was resuspended in passage M199 medium (M199 medium with antibiotic cocktail plus 10% FBS and endothelial mitogen) and seeded into new fibronectin-coated culture tissue flasks until confluent. HUVEC passaging was made by using population doubling, i.e., passage of one flask into two flasks. This process was repeated twice more for third-passage HUVEC.
ELISA for cell surface molecules.
ELISAs were carried out as described (20). In brief, HUVEC were seeded at confluence into fibronectin-coated wells in a sterile 48-well plate (Costar, NY). Once confluent, the cells were left untreated or stimulated with smooth E. coli O111:B4 LPS for 4 h at 37°C. The cells were washed with ice-cold PBS, fixed with 1% ice-cold formaldehyde, and blocked overnight in 1% BSA (Sigma). The cells were then labeled with either 10 μg/ml for CD14 or 12 μg/ml for E-selectin MAb (incubation for 30 min) and washed with PBS. Incubation with peroxidase-labeled goat anti-mouse IgG (10 μg/ml) was used to label these monoclonal antibodies; the cells were then washed. Color was developed with tetramethyl benzidine one-step substrate system (Sigma). The color reaction was stopped with 0.2 M sulfuric acid, and the supernatants were transferred to a 96-well plate. The optical densities (ODs) were read on a plate reader at 450 nm (Software Spectra Max plus, Molecular Devices; software SOFTmax Pro; version 3).
Monocyte enrichment.
Peripheral blood mononuclear cells (PBMC) were isolated from blood by using Histopaque 1077 (Sigma). The buffy coat layer was collected, washed in PBS, and incubated for 2 h at 37°C in a tissue culture flask. The cells were washed to remove floating cells, and adherent cells were treated with 0.05% Trypsin-EDTA (GIBCO, Invitrogen).
Flow cytometry.
Cells were removed from culture flasks by using 0.05% Trypsin-EDTA and washed in cold PBS. Cells were aliquoted into 1 × 106 cells per flow tube and kept on ice. Appropriate antibodies (20 μl per flow tube) were added to the cells and incubated in the dark and on ice for 30 min. Cells were then washed with cold PBS and either fixed with 1% formaldehyde or read immediately. The cell populations were gated to avoid debris and analyzed on a flow cytometer (BD FACScan cytometer using Cell quest pro software).
RT-PCR and real time RT-PCR.
HUVEC, BJAB cells, and matured U937 cells were washed with sterile PBS, and total RNA was isolated (RNeasy kit, Qiagen), dissolved in diethyl pyrocarbonate (DEPC)-water, and reverse transcribed (Omniscript, Qiagen). PCR analysis was performed with the following specific primers: GAPDH as described (25); CD14 fragment as described (34); CD14 1 kbp, forward primer 5′ ACC ACG CCA GAA CCT TGT GAG 3′ and reverse primer 5′ CAG CAC CAG GGT TCC CG 3′; and CD18 primers as described (18). PCR conditions included a 94°C hot start for 4 min followed by 35 cycles of denaturing at 94°C for 1 min, annealing temperature at 55°C (for CD18 and GAPDH) and annealing temperature 60°C (for CD14 and GAPDH), and extension at 72°C for 2 min. Samples were run on a 1% agarose gel containing ethidium bromide. Real-time RT-PCR was carried out with the use of the same CD14 fragment primers and GAPDH primers as for the RT-PCR. Semiquantitative analysis was performed by monitoring in real time the increase in the fluorescence of the SYBR-green dye (Molecular Probes) on a Bio-Rad i-Cycler (Bio-Rad Laboratories, Hercules, CA). Proper amplification was confirmed by performing melting curve analysis. Real-time fluorescence measurements were performed, and a threshold cycle value for each gene of interest was determined as previously reported (12). All data were normalized against the GAPDH mRNA level and expressed as fold increases relative to positive control, matured U937.
Polymyxin B treatment of LPS.
Polymyxin B (PmB) (Sigma) was incubated at a concentration of 5 μg/ml with smooth E. coli O111:B4 LPS for 20 min at room temperature before the treated LPS was added to HUVEC.
Flow chamber assay.
To study LPS-induced leukocyte recruitment on HUVEC under shear conditions, a laminar flow chamber was used as previously described (20). Fibronectin-coated glass coverslips with confluent monolayers of HUVEC were mounted into a polycarbonate chamber with parallel-plate geometry. The flow chamber was placed on an inverted microscope stage, which was enclosed with a warm air cabinet, and the temperature was maintained at 37°C. A syringe pump (Harvard Apparatus) was used to draw freshly donated heparinized blood from a healthy donor over monolayers at a shear of 2 dyn/cm2. The blood drawing protocol was approved by an independent University Ethics Committee. The tissue was then perfused with warm HBSS to enable visualization of the leukocytes (HBSS has no added phenol red). Interactions of leukocytes with the endothelium were visualized and recorded by using phase-contrast microscopy (×100 magnification). Transendothelial leukocytes were observed as phase dark cells (×200 magnification). The microscope was linked to a VCR machine, and video tapes of the experiments were recorded for later play-back analysis. Interactions were analyzed for rolling cells (defined as leukocytes moving at a slower rate than red blood cells), adhering cells (cells stationary for 10 s or longer), and transendothelial cells (phase dark cells).
PI-PLC treatment of cells.
PI-PLC (Sigma) was added into the corresponding tissue culture medium and incubated with cells for 1 h at 37°C. To determine the effective concentration, the PI-PLC was titrated from 0.001 U/ml up to 0.1 U/ml on matured U937 cells (see Fig. 7A). PI-PLC at 0.1 U/ml was used for all subsequent experiments.
Statistical analyses.
Values are expressed as means ± SE. Where appropriate, results were analyzed for statistical significance by using a Student's t-test. A P value of <0.05 was taken to be significant.
CD14 is present on primary-passage HUVEC but not tertiary passaged cells.
We examined whether CD14 is present on monolayers of cultured primary-passage HUVEC. Furthermore, we investigated the impact of cell passage on this expression (Fig. 1). Two flasks of HUVEC were cultured from the same umbilical cord, and cell surface expression for CD14 was examined by using a cell-surface ELISA at first and third passage. On incubation with the CD14-specific antibody on first-passage HUVEC, a fourfold increase in CD14 was observed compared with the isotype control. In contrast, when the same antibody was incubated with tertiary-passaged HUVEC, there was no detectable CD14 compared with the isotype control. In addition, there was ample mRNA for CD14 in primary HUVEC but not tertiary HUVEC cultures (data not shown). These results demonstrate that CD14 is expressed on primary-cultured HUVEC but not tertiary-cultured HUVEC. Because both primary and tertiary HUVEC were incubated in the same FBS, the CD14 detected is unlikely to be residual soluble CD14 from the culture medium.
Primary HUVEC are able to express a high percentage of CD14.
To further characterize the level of CD14 expression on primary-passage HUVEC, we compared percentage and mean fluorescence of CD14 expression of endothelium and monocytes. Monocytes are considered to express high constitutive levels of CD14. Figure 2 reveals that a very significant proportion of both monocytes (Fig. 2A) and endothelium (Fig. 2B) displays CD14 on the membrane surface. The histogram profiles from the flow cytometry data demonstrate the variability of CD14 on HUVEC from the same cord (Fig. 2B). Figure 2, C and D, shows that on average 92% of monocytes and 50% of HUVEC were positive for CD14, respectively. The density of CD14 per cell was analyzed, and monocytes showed average mean fluorescence intensity (MFI) of 90 compared with an MFI of 49 on HUVEC. Therefore mean expression per HUVEC was approximately half of mean expression of monocytes. Interestingly, the highest expressing HUVEC had an MFI of 124, which is more than the highest expressing monocytes, which had an MFI of 97 (Fig. 2, E and F).
HUVEC are capable of synthesizing CD14.
There are reports in the literature to suggest that molecules can originate from one cell type and transfer on to another cell type (5, 15). To examine if HUVEC are producing CD14-specific RNA, RT-PCR was carried out (Fig. 3A). A band corresponding to the expected 425-bp product from the CD14 primers was detected in four different HUVEC samples (Fig. 3A, lanes 5–8), as well as in a matured monocyte cell line (Fig. 3A, lane 4). There was minimal product in a B cell line (BJAB) consistent with low expression (Fig. 3A, lane 3), and the water control showed no contamination (Fig. 3A, lane 2).
HUVEC synthesize large amounts of CD14-specific mRNA.
To demonstrate that there are physiological quantities of CD14-specific mRNA present in HUVEC, a comparison was made of the quantities detected compared with a matured monocyte cell line (Fig. 3B). Real-time RT-PCR analysis for three different HUVEC samples showed CD14 mRNA at similar and even higher levels compared with the matured monocyte cell line control. Again, variability was noted. BJAB expressing low levels of CD14 protein confirmed a minimal amount of CD14-specific mRNA.
The specific CD14 mRNA detected was not due to contaminating monocyte mRNA.
To ensure that the specific CD14 bands observed in endothelium were not due to contamination of blood cells during the isolation procedure, PCR was carried out on the same cDNA (Fig. 3C) to detect CD18 message, which is known to be present on leukocytes but not on HUVEC. The primers used were designed to amplify a 462-bp fragment of CD18. The results from the RT-PCR demonstrate that there was no CD18 message detected in the HUVEC total RNA (Fig. 3C, lanes 5–8) compared with the positive controls U937 (Fig. 3C, lane 4) and the B cell line (Fig. 3C, lane 3). The water controls were negative (Fig. 3C, lane 2), demonstrating that no other contamination was detected. These data demonstrate that the HUVEC lysates used were not significantly contaminated with leukocytes and so could not account for the CD14 mRNA detected.
The specific CD14 protein detected was not due to contaminating monocytes.
To ensure that contaminating CD14 protein from infiltrating cells was not contaminating the endothelium, we again made use of the fact that monocytes and other leukocytes are CD18 positive, whereas HUVEC is CD14 positive but CD18 negative. Flow cytometric analysis was carried out, and Fig. 4, A and B, demonstrates our gating strategy to select for monocytes from whole blood and HUVEC. While the majority of our monocytes were double positive for CD14 and CD18 (Fig. 4C), <1% of our HUVEC were double positive (Fig. 4D) compared with the isotype controls (Fig. 4, E and 4F).
The amino acid sequence of HUVEC CD14 is 99% similar to that of monocyte CD14.
A comparison was made between the sequences of HUVEC CD14 and monocyte CD14. We designed primers that amplified a region from amino acid 37 to amino acid 347 and sequenced the PCR product. The data show that there is a 99% homology between the detected CD14 sequence on HUVEC compared with the published monocyte sequence (www.ncbi.nlm.nih.gov accession number NM_000591.1) (data not shown).
Endothelium can detect LPS in the absence of plasma proteins.
LPS invokes a response in endothelium (E-selectin synthesis). Figure 5A demonstrates that at an LPS concentration range from 0.01 to 0.1 ng/ml E-selectin expression was not detectable in the absence of plasma. By contrast, addition of plasma allowed LPS responses at 0.01 and 0.1 ng/ml of LPS. However, at 1 ng/ml of LPS a large response was detected in the absence of plasma compared with the isotype control. Maximum levels of E-selectin expression occur at an LPS concentration of 1 ng/ml or above in the absence of plasma, and addition of plasma did not increase maximal responses to LPS in this range.
The lipid A domain of LPS is the critical factor in activating endothelium.
To study the specificity of the endothelial response to LPS, the critical activation domain, lipid A, was inhibited using PmB. Figure 5B demonstrates that the endothelial response to LPS was completely blocked by PmB. By contrast PmB had no effect on TNF-α-induced responses, suggesting an absence of nonspecific effects of PmB on endothelium. It is worth noting that high concentrations of PmB caused nonspecific cellular injury, making the TNF-α control very important.
Primary-passage HUVEC can recruit leukocytes in the absence of plasma proteins.
To examine if the E-selectin endothelial response to LPS observed on ELISA was physiologically relevant, leukocyte interactions with endothelium were quantified. Figure 6 shows increased rolling, adhesion, and emigration (Fig. 6, A–C, respectively) in HUVEC stimulated with LPS in the absence of plasma proteins. Figure 6 also shows that pretreatment with a CD14 blocking antibody completely inhibited the LPS response for all three parameters investigated. This antibody did not affect TNF-α-induced leukocyte rolling and adhesion (data not shown). Addition of plasma to the CD14-inhibited HUVEC reconstituted ∼30% of the LPS response, suggesting that there are proteins in plasma that can function as a surrogate for membrane CD14 (data not shown).
CD14 is attached to the endothelium via a GPI anchor.
To establish whether the CD14 detected by both ELISA and flow cytometry was fixed to the HUVEC cell surface and not due to contaminating soluble CD14 trapped on the HUVEC cell surface, HUVEC were treated with GPI-specific PI-PLC. GPI-specific PI-PLC has been previously used to inhibit GPI-linked CD14 on monocytes (32). Figure 7A shows that 0.1 U/ml PI-PLC removed known GPI-anchored CD14 on PMA-treated U937 cells. This same optimal concentration of PI-PLC also removed CD14 from HUVEC (Fig. 7B). Figure 7C shows that a transmembrane (non-GPI anchored) HUVEC surface protein, CD54 (ICAM-1), is not removed by PI-PLC. Finally, to ensure that the PI-PLC reagent was not contaminated with LPS, thus causing the downregulation of CD14, the PI-PLC was boiled to inactivate the enzyme. Once inactivated, the PI-PLC failed to remove CD14 expression (Fig. 7D).
Removing CD14 from the HUVEC surface is associated with a reduction in the HUVEC response to LPS.
HUVEC were treated with LPS with or without prior treatment with GPI-specific PI-PLC (to remove GPI-anchored proteins, including CD14). Figure 8 demonstrates that HUVEC stimulated by 10 ng/ml of LPS caused the expression of E-selectin to be increased fourfold compared with either isotype levels or levels of E-selectin expression on untreated HUVEC. In HUVEC treated with PI-PLC to remove CD14, there was a reduction of E-selectin expression.
Over the past 10–15 years, it has become clear that neither passaged endothelium nor endothelial cell lines have the capacity to respond to LPS in the absence of plasma proteins. However, in the presence of plasma proteins and more specifically soluble CD14, endothelium responds to LPS by expressing adhesion molecules to allow for leukocyte recruitment. From this work it has become generally accepted that endothelium does not constitutively express CD14 but requires external sources of soluble CD14. However, for many years, numerous investigators using primary or first-passaged endothelium stimulated with LPS observed a robust adhesive response of leukocytes to LPS-stimulated endothelium (24, 30, 31). These LPS responses were observed in the absence of plasma, raising some potentially conflicting data. Additionally, von Asmuth and colleagues reported that anti-CD14 antibody blocked endothelial responses to LPS even in the absence of serum (30). Although these data do suggest the possibility that endothelium could express CD14, an alternative explanation could be that soluble CD14 from the fetal bovine serum in which the endothelium was grown was incorporated onto the surface of endothelium. However, quite recently, Jersmann and colleagues (11) reported CD14 immunoreactivity on the surface of endothelium that was lost upon passage, an observation that was suggestive that perhaps endothelium may have its own CD14. Indeed, we present data herein that demonstrate CD14 message and protein associated with endothelium. The CD14 was not due to contaminating leukocytes. Most importantly, removal of GPI-linked proteins from the endothelial surface eliminated CD14 immunoreactivity, suggesting for the first time that it was the GPI-linked membrane CD14 that was on endothelium and functionally could induce adhesion molecule expression as well as recruit leukocytes to the endothelial surface.
The endothelium as the essential cell for leukocyte recruitment is well established. If endothelium is not activated, leukocytes cannot be recruited to the leukocyte-endothelial interface regardless of the state of activation of the leukocytes. Under flow conditions, there is a requirement for endothelial activation by LPS, leading to E-selectin synthesis and subsequent leukocyte recruitment. Our data demonstrate that in the absence of any exogenous source of CD14, endothelium has the capacity to detect low levels of LPS (1 ng/ml), leading to E-selectin synthesis and leukocyte recruitment. Moreover, the endothelium has message for CD14, and Jersmann et al. (11) were able to reduce expression of endothelial CD14 by inhibiting protein synthesis. By blocking CD14 function with PI-PLC, a molecule that removes all GPI-linked proteins from the cell membrane surface, we also support the view that the endothelial CD14 is the membrane form found on monocytes. Finally, removal of this CD14 inhibited endothelial E-selectin production and leukocyte recruitment, further supporting a functional role for membrane CD14 on endothelium.
Our data are also entirely in line with Frey and colleagues (4) who reported that at very low concentrations of LPS (1 ng/ml or less), endothelium was unable to detect LPS without the presence of plasma and more specifically soluble CD14. Our data also demonstrate that plasma was required to detect 0.1 ng/ml LPS; however, we cannot discount the possibility that membrane-bound CD14 is necessary for this process. In this regard, two studies have reported that serum was not sufficient to induce LPS responsiveness in cells devoid of membrane CD14, but on transfection of CD14, LPS responsiveness (in the presence of plasma) was then restored (7, 17). One possibility may be that soluble CD14 transfers LPS to membrane CD14. Indeed, Hailman et al. (8) reported that soluble CD14 can facilitate rapid transfer and efficient presentation of LPS to membrane CD14. However, preliminary data from our laboratory (data not shown) suggest that following inhibition of membrane CD14, plasma can reconstitute ∼30% of the LPS response. These data would suggest that plasma independent of membrane CD14 can directly aid in endothelial LPS detection.
As previous studies have shown endothelium to be negative for CD14, we investigated whether the absence of CD14 was due to passaging of HUVEC. Our results demonstrate that CD14 expression on endothelium is indeed lost on passaging, an observation consistent with one other study (11). Passaged HUVEC are routinely used in many laboratories, and the loss of expression of surface molecules due to passaging of endothelium has been observed for other proteins, including von Willibrand factor and P-selectin (13, 19). We also report that CD14 expression is heterogeneous on HUVEC. Indeed, we have seen heterogeneity of CD14 expression in our samples, which range from as high as 85% to as low as 20% of that detected on monocytes (Fig. 2D). It is well known that monocytes vary in their expression of CD14. Interestingly, variable CD14 expression has also been reported on human ginigival fibroblasts from different sources as well as the same source cultured in separate flasks (28). Therefore, variable CD14 expression on HUVEC could be due to time of culture or maturation state. Alternatively, it could be due to differences within the human population, as suggested by Sugawara and colleagues (28). Nonetheless, our data clearly show that CD14 protein can be detected on endothelium.
Previously, it has been suggested that the plasma protein LPS binding protein (LBP) is also required for endothelium to respond to LPS. Analysis of the LBP knockout mouse has revealed less sensitivity to LPS, but nevertheless a potent response is still seen (33). It has been hypothesized that LBP is required for CD14-negative cells to respond to LPS (3). Our data would suggest that LBP is not essential for LPS concentrations of 1 ng/ml and higher because all these responses were inhibited with a CD14 MAb, as well as GPI-linkage inhibition. Nevertheless, we do not discount the possibility that LBP, much like soluble CD14, could increase sensitivity of endothelium to LPS.
This is the first report that endothelial CD14 is linked to the membrane via a GPI anchor. This study suggests that there is functional, GPI-linked CD14 on endothelium, in contrast to the generally accepted view that CD14 is absent from endothelium. Moreover, we support the view that a lack of CD14 on endothelium likely reflects issues related to cell culture rather than a physiological phenomenon. In this study, we show that primary HUVEC cultured in the absence of mitogen express GPI-linked CD14 and that it originates from the endothelium. The identification of endothelial CD14 is important as it further suggests endothelium as a critical cell in the inflammatory response.
This work was supported by a Canadian Institutes of Health Research (CIHR) operating and group grant and the Calvin, Phoebe and Joan Snyder Chair in Critical Care Medicine. P. Kubes is a Canadian Research Chair and an Alberta Heritage Foundation for Medical Research scientist.
The authors have no financial conflict of interest.
We thank the staff at Foothills Hospital Maternity Ward for supplying the umbilical cords, and we also thank our healthy blood donors.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2006 by the American Physiological Society

subset: commoncrawl


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Holiday cottage, Rye, East Sussex
November 2017
Ockman Cottage
"Great location in centre of Rye. Close to all amenities but in a quiet side street location. Also good location for visiting Kent and East Sussex coasts. Several good walks from cottage including great circular walk to Winchelsea via coast and Camber castle. Lots of great places to eat in Rye. Ideal location for a short break. Cosy cottage with good facilities."
October 2017
lovely cottage
"Every thing was ok we were like at home. The owner called us at our arrival to ask if everything was fine. Nous recommandons le cottage et la petite ville de Rye "
August 2017
Very pleased with Ockman Cottage
"We were delighted with our stay in Ockman Cottage. The cottage is right in the middle of the historic town but because it is tucked away it is really quite and peaceful. The cottage was beautifully decorated and really well equipped. We really enjoyed our stay and will definitely come again."
August 2017
Beautiful place for a short break in the centre of a vivid town
"The cottage is in a well maintained and fully equipped condition, especially the kitchen has more than you typically need during vacation. The beds and sofas were a bit too soft for our taste. What we liked very much was the cleanliness of the place. "
May 2017
Welcoming and comfortable cottage right in the centre of Rye
"This was a really comfortable and welcoming cottage, perfect for us as a couple. It is very well equipped and everything works well. Location is fantastic, right in the centre of the citadel. "
December 2016
Christmas 2016
"We stayed as a family of 3 at Ockman Cottage for 5 nights over Christmas. It is in an idyllic location in the centre of Rye, but so quiet. Perfect for us, spotlessly clean and cosy. Even cooked Christmas dinner in the compact, but well equipped kitchen. The owner was extremely helpful, contacting us prior to our stay. Would definitely return."
October 2016
Very quaint cottage, excellent position for a visit to Rye
"Our stay was excellent, Ockman Cottage is a real jewel in the centre of Rye. Everything has been thought of to make your stay very relaxed. The beds are extremely comfortable, and the d?cor throughout has been tastefully done. Would recommend a visit to Ockman cottage to see for yourself."
You enter the property from the private cobbled twitten, into a spacious open plan living area. The property elegantly mixes period pieces with modern touches, giving the holiday cottage the feel of a boutique hotel. The kitchen is fully equipped and whether you choose to eat in, or visit one of Rye's many lovely eateries, the choice is yours. The lounge has comfy sofas, ample lighting and a flatscreen TV for nights spent in, relaxing with a movie. The cottage has a selection of local maps for planning leisurely walks and various guides to help you decide how to spend your days. There is a small outside courtyard with planted pots and seating.
Stairs lead to one kingsize bedroom and one double bedroom and a bathroom. Both bedrooms have shuttered windows, luxury headboards and colour-complementing soft furnishings, which give the property a luxurious appearance. The bathroom has a bath, overhead shower and heated towel rail.
On arrival a welcome pack of wine, biscuits, coffee, tea and milk will be waiting for you to get your holiday off to a good start. The owners have tried to think of everything to ensure your holiday is as relaxing and problem free as possible.
Ockman Cottage is the perfect base for exploring everything Rye and the surrounding 1066 Country has to offer.
A gentle walk and you can visit the Heritage Centre where you can watch the town model ' Sound and Light' show, which goes into detail about the history and the colourful past of Rye and its inhabitants, a good start to any holiday in Rye. Visit the 'Old Pier' working models upstairs and play the old penny machines and step back in time. Or amble around the many antique shops in the Strand Quay and hunt out a bargain!
The town of Winchelsea is a ten minute drive away and, like Rye, has a rich history and links to the days of smugglers. One of the many cellar tours that take place during the year is a worthy visit and a chance to see parts of the town that are not normally on show. With far reaching views over the marshes and out to sea, it really is a pleasant way to spend a few hours.
Just ten minutes drive away is the Rye Harbour Nature Reserve, where you can walk along the sea wall and out on to the marshes to enjoy the flora and fauna. You can choose to walk out to Camber Castle, originally located on a shingle spit and where once it protected the towns of Rye and Winchelsea before the sea receded. Although no longer open to visitors, it is still a striking landmark worth a visit. The beach of Camber Sands is just a short drive away and has miles of sandy beach and dunes to explore. Or visit the rock pools at low tide on the pebble beach at Pett Level, just a ten minute drive from Rye.
The towns of Hastings and Ashford are around a thirty minute drive away and here you can get mainline trains to London if you wish to explore the capital for a day. If you would like to take a day trip to the continent, the Eurostar can be picked up from Ashford International Station, and trains to Paris, Lille and Bruges are available. Alternatively, the ferries run from Dover, just 45 minutes away or the Channel Tunnel from Folkestone, a 45 minute drive.
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Oh dear. That sinister Doomsday Clock has just ticked forward 30 seconds. The Bulletin of Atomic Scientists cites Trump and climate change ("which I had hoped would cancel each other out", said Giles Coren in The Times). Now we're at only two and a half minutes to midnight, the closest we've been to disaster since 1953. This certainly chimes, as it were, with the zeitgeist. There is unquestionably "a sense out there that the end of the world is nigh", says Coren. "It's all over Twitter and the liberal press, it's there in the glum faces of your friends at dinner and the black humour on metropolitan coffee shop blackboards…"
In America, the novelist Philip Roth wrote last week: "What is most terrifying is that [Trump] makes any and everything possible, including, of course, the nuclear catastrophe?" Meanwhile, in the current edition of The New Yorker, there is a piece headlined "Doomsday Prep for the super-rich" in which the magazine examines how some of America's wealthiest people are "getting ready for the crack-up of civilisation".
We hear about Steve Huffman, the 33-year-old co-founder of Reddit, who is worried about "the temporary collapse of our government and structures". Huffman, whose company is valued at $600m, lives in San Francisco. "I own a couple of motorcycles," he says. "I have a bunch of guns and ammo. Food. I figure that, with that, I can hole up in my house for some amount of time."
Survivalism, says Evan Osnos, the New Yorker writer, may once have conjured up a picture of hillbillies: "the woodsman in the tinfoil hat, the hysteric with the hoard of beans, the religious doomsdayer". But the new survivalists are hi-tech wizards or hedge-fund mangers, living in places like Silicon Valley or New York.
Antonio García Martínez, for example – a 40-year-old former Facebook product manager – has bought five acres of woodland on an island in the Pacific Northwest and brought in generators, solar panels and thousands of rounds of ammunition. The author of a book called Chaos Monkeys, he wanted a refuge that would be away from cities, but not too isolated.
"All these dudes think that one guy alone could somehow withstand the roving mob. No, you're going to need to form a local militia. You just need so many things to actually ride out the apocalypse." Once he began telling people in San Francisco about his island project, they "came out of the woodwork" to describe their own schemes. "I think people who are particularly attuned to the levers by which society actually works understand that we are skating on really thin ice right now."
Another survivalist, a tech fund manager, tells Osnos he keeps "a helicopter gassed up all the time" and that he has "an underground bunker with an air-filtration system. A lot of my friends do the guns and motorcycles and the gold coins. That's not too rare any more." The motorcycles come from a 1998 film, Deep Impact, in which a boy and his girlfriend escape an asteroid attack on a motorbike. Giles Coren says he doesn't own a helicopter and can't ride a motorbike. He'll just have to make do with his bicycle and his air rifle.
Tabloid money… "Cheer up, grow up and shut up, Emma"
• "Let me stress immediately that bribery and corruption are morally wrong," says the Daily Mail's Peter Oborne. But John Rose, the boss of Rolls-Royce caught up in a "deafening chorus of denunciation" over "the revelation that the firm has owned up to 'extensive systematic bribery and corruption'", is a patriot, who "should be celebrated rather than pilloried". Rose's critics should keep in mind "that there are vast areas of the globe… where bribery is standard practice".
Indeed, some cultures do not even see it as wrong, says Oborne. Put simply, Rose faced a choice between allowing Rolls-Royce to be destroyed by foreign rivals who did not have to abide by such niceties as Tony Blair's anti-bribery law, "or devise a way of dealing with it".
• Donald Trump had a point when he said he wrote things down because he did not trust computers, says Frederick Forsyth in the Daily Express. These days, "confidential information might as well be on a hoarding on the M1". Banks are forever telling me to "be modern and bank online". But "if every con man can hack in", why bother? "As for security, I have been issued so many personal IDs, passwords, PIN numbers… I could set up my own GCHQ". And when it comes to bank helplines giving information, "Captain Mainwaring was faster in Dad's Army", says Forsyth. "So the Hairdo in the White House may have it right. Jot it down."
• Actress Emma Watson (pictured) is reported to be "freaking out" because she turned down a role in La La Land, the stars of which are tipped for Oscars, says Ann Widdecombe in the same paper. "If that is true, then all I can say is calm down, dearie." "First Emma, you are only 26… Second, you are already a multi-millionaire and an acclaimed actress, while so many talented people are struggling." Besides, making bad calls is part of grown-up life, says Widdecombe. "So cheer up, grow up and shut up, Emma."


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Wednesday Words
To Go Where Your VisionPoints, a few inspiration points for you and your business.
There are three kinds of men. The one that learns by reading. The few who learn by observation. The rest of them have to pee on the electric fence for themselves.
—Will Rogers
If you didn't find your must-have summer reading on Monday, you'd better hone your powers of observation! This week and next, it's all about little observations here at Maximum Customer Experience. Let's have some fun with it.
Grow and be well,
Kelly Erickson
8 Responses to "Inspiration Points: If You Don't Feel Like Reading (ZAP!)"
I've never had the pleasure of peeing on an electric fence myself. But I'll take Will Roger's word for it….
Friar's last blog post…Friar's Random Rants (Part III)
I did stumble over an electric fence, back in my younger years. We were tramping through Belgium near the Battle of Waterloo site and for some reason decided to race down some hill or other. (My buddy and I were always racing — perhaps a guy thing in your early 20s?)
We got to the bottom and there was this thin wire sort of like fishing line but a bit wider. He hopped over, but of course I had no idea what it was and grabbed it.
It was low voltage to keep the cows in, but still quite a shock (literally) when you're not expecting it.
Can't say I learned anything though. My buddy and I are still idiots when we get together…
Graham Strong's last blog post…One Dime Is All It Cost: How to Keep Your Content Current (And Why You Should…)
Ah! So you are a reader! An excellent path to choose. 🙂
If you weren't the first commenter today I hope you know I would have been very disappointed in you.
Will Rogers: Good lessons, told so oddly you can't possibly forget them. Love how his brain worked.
Glad to see you so early in the a.m.!
You sneaked under as I was typing. Sounds like some electric fences teach cows more than people. I'm with Friar, though–I'd rather take your word for it.
"There are three kinds of men. The one that learns by reading. The few who learn by observation. The rest of them have to pee on the electric fence for themselves."
…and those who just don't seem to learn at all. Even after they've zapped their you-know-what!
I don't learn by reading. I definitely have to see it done. Then I have to try it myself. Never tried the peeing on fence thing, but I am a girl, so that might be more challenging. I have been known to get my tongue stuck to a frozen metal post!…oh dear…nobody's going to take that and run with it are they?
@Urban Panther
Well, it could be worse…you could have put your tongue the the electric fence…:-)
Friar's last blog post…Friar's Random Rants (Part III)
I run a nice calm joint here. When we want to run with things like that, we check if your man's been shaving anything new lately, and run over there.
Will Rogers may not have thought about the logistics, but I do think he meant men in the sense of "folks," not in the sense of "people with you-know-whats."
I'd say we're going to have to put you in the third category, for freezing your tongue in that manner. Worth noting that he didn't actually say which category he belonged in…
I think he's right, by far the biggest category is those who've just got to try it even though they know they may get zapped. Under certain circumstances, not involving frozen posts or electric fences, it's probably what moves society forward. 🙂
Friar, LOL!


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Linklaters is emerging as a frontrunner to win the mandate advising on the IPO of AIG's Asian life assurance arm.
The magic circle firm will take part in a beauty parade to advise on the deal, which AIG hopes will raise between $5bn (£3.27bn) and $10bn through the IPO of American International Assurance (AIA) in Asia.
In February, Linklaters Hong Kong partner Chris Kelly led a team advising AIG on the sale of its consumer finance business in Asia to Ayudhya. Allen & Overy partner Suparek Auychai advised Ayudhya on the acquisition.
Linklaters declined to comment on the deal, but a source close to the firm said: "Counsel for the AIA IPO is yet to be decided. There's a process and we expect there to be a beauty parade."
Several firms have won instructions from AIG since it was bailed out by the US government in September 2008.
Since then the insurance company has established a global steering group comprised of AIG executives and private practice firms advising on the sale of aspects of the business.
Sullivan & Cromwell and Weil Gotshal & Manges are both part of the steering group. Weil New York partner Michael Aiello is one of the partners representing the firm on the steering group.


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Category: Stock Graphic, Icons
Stock Graphic >> Icon - Round food icons 165023 by miumiu @ CreativeMarket
File Size: 3.25 MB; Requirements: Adobe CS1+; Layered: Yes; DPI: 72 DPI; Vector: Yes; Dimensions: 64 x 64 px96 x 96 px128 x 128 px.
Collection of round flat medical icons
You can scale icons without loosing quality, change colors, mix them together. Perfect for your blog, website, flyers or labels
- 2 EPS8 files with all icons with and without shadow
- 84 PNG files with icons in two styles (64x64px, 96x96px, 128x128 px)
- 4 PNG files with preview
You can find more sets of icons in my collections: "Hand drawn icons", "Flat icons", "Outlined icons" and "Round icons"
- https://creativemarket.com/miumiu/collections/44739/Hand-drawn-icons
- https://creativemarket.com/miumiu/collections/62524/Flat-icons
- https://creativemarket.com/miumiu/collections/62527/Outlined-icons
- https://creativemarket.com/miumiu/collections/78500/Round-icons
Keywords: food, fruit, vegetables, icons, round, circle, cheese, ham, mushroom, apple, cucumber, bread, tomato, carrot, loaf, pear, fish, milk, beet, cherry.


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Thursday, December 29, 2011
The Girl with the Dragon Tattoo--Movie
Photo: Rooney Mara, from her Wikipedia page. She and Lisbeth Salander look nothing alike. I mean that in a positive way, for both.
The blog for the book will come soon. Just finished it.
But first the movie.
The movie was a must-see on many levels: the genre of the movie and book (as you should know by now, I dig the murder/mystery thing); the positive hype of both; and, perhaps most importantly, David Fincher, the director.
First, a word about him. Impressive credits, especially Zodiac. I know he directed Se7en, a good movie but very overrated. The Social Network was a great film, about technology that has undoubtedly changed many lives (except mine; still don't have a Facebook account)--but, still, a movie about a nerdy, ingenious, socially inept guy, who may or may not have stolen the idea, the rights, and who knows what else. Not my cup of tea for a subject, but an admittedly great film. I believe Zodiac and now this movie will push him into the upper-echelon of movie buffs (Fincher's been there for the pros for some time now). I like Fincher's directing style and the intensity of his films: The Social Network was intense for its genre. The others are just...intense. Fight Club, for instance. (Good, not great.) Haven't seen Benjamin Button or Panic Room. They're on my list of things to do.
I also wanted to see this because Steven Zaillian wrote it. He penned the 'plays for Schindler's List (one of the all-time great screenplays) and for Gangs of New York, also a great screenplay (Daniel Day-Lewis should've won the Oscar for that one). Of course, he also wrote American Gangster, which was okay, but I'll pass, and Hannibal, which was just bad. But I'm a return customer for Schindler and Gangs.
Daniel Craig is always good, and it's good to see him do something well besides James Bond. (Speaking of which, it's about time for another, yes?) He's a good actor who has not, and will not, get the credit he deserves. Women swoon over him. While he's essentially a non-violent Bond in this film, he still played it well. He's got the Bond charm and charisma going, but he still had other facets going on that went over well. (Those shots where he's learning something, and the camera stays on his thoughtful and learning face--well, rumor has it that he was simply counting to ten all that time. He says he counts to ten when he's told he needs to show understanding and learning on film.)
But the cream of the crop here was Rooney Mara, as Lisbeth Salander. According to Wikipedia, she beat out Natalie Portman and Scarlett Johansson for the role. I like the first and am lukewarm by the latter, and they're both too hyped these days. Mara came out of nowhere. She played bit parts in several TV shows, a major part in the Nightmare on Elm Street remake (all good actors have to start with a bad horror film, apparently. See Johnny Depp, Renee Zellweger and too many others to mention), and a small role in Fincher's Social Network. (Both say that her part in his most recent film did not help her to get The Girl.)
Her character on film is even more realistic than the one on paper I have just finished reading. That never happens, at least for me. Without going into much detail, she absolutely nailed this role. One of the things she did in the film (and possibly this is Fincher's and Zaillian's doing as well) that Salander didn't do in the book was to stay consistent with her mono-syllabic answers and otherwise clipped speech patterns--even to those she liked or cared for. Frankly, she spoke too often (in spurts) in the book; I have known people (exactly) like Lisbeth Salander, and you'll have to take my word that such folks do not normally converse. They staccato you, or they rant at you, but they don't converse. Mara didn't in the movie, either.
In my life, I have seen a Lisbeth Salander many times. I've seen the anorexia, the internal fury, the speech patterns, the behavior, the anger in the eyes, the massive insecurity, the abuse, the addictions--In short, I've seen it all. She owned it. This movie should be seen for her performance alone. (I dare you to recognize the woman in the film as the one pictured above, but they are the same.)
Sweden itself gives a good performance, too, if you know what I mean. (I know about 40% of the internal shots were filmed in L.A.) It's obviously beautiful and cold there. The free education intrigues me, but the violence and rape stats in this trilogy--and in the many books by Henning Mankell--do not paint a pretty picture. Both authors (I know Larsson has died) try to say that the stats are horrific, possibly even worse than the U.S.'s. For now, I'd like to visit there, but not live there. Of course, I can say that about almost anywhere.
And the movie has a lot of nice touches not in the book, which I'll leave alone for now. Suffice it to say that they involve the whereabouts of a missing woman and a car crash. The movie also breaks a few rules; the big one is that the movie, like the book, continues on for quite awhile after the main mystery has been solved. I approve, especially because it is done largely for the sake of characterization. Rare, these days.
More on this, and the book, later. Go see it, even if you've already read it. And I'll be proactive and say that you should read it, too, even if you've already seen the movie.
10 comments:
The book has been on my "To Read" list for some time. I keep passing it up solely because I own it - giving my attention to the library books with due dates instead. Since I hate seeing movies before I've read the book, guess I'll have to send it forward on the queue. Thanks for the great review.ReplyDelete
No problem, Namzola. The actual book review is in a blog entry above, from January 2nd. Definitely read the book first before you see the movie, but both are worth doing. And I hear ya about reading the overdue books first. I used to have plenty of those, too.ReplyDelete
Have you seen the Swedish version?ReplyDelete
My boss is from Denmark, and one of his huge pet peeves is Hollywood remakes of really good foreign films. It drives him bonkers--he just can't understand why Americans, unlike the rest of the world, can't just watch and appreciate the original films in their original languages with subtitles. His perpetual example is a really good Danish film called "Brodre," absolutely excellent in the original, then Hollywood did a remake called "Brothers" with Jake Gyllenhaal and Tobey Maguire--and in my bosses opinion it was "eh."
Before Christmas he was going on and on about how annoying it was that the fantastic Swedish films for the "Dragon" trilogy were being remade. "What's wrong with the original? Can't Americans read subtitles?"
I haven't read the "Dragon" series yet. I'm one of those weirdos who avoids book fads (gasp, I've never read "Harry Potter"), at least until the hype dies down. However during Christmas 2010 P and I were stuck at his brother's apartment in Philly after the Dec 26th storm, and we were looking through our Netflix account for something to watch. It was streaming the first two "Dragon" Swedish films, and I thought, "hmm, maybe these will be interesting..." We intended just to watch one, but got so sucked in to the story, we started the second one right away even though it was 2am. We had to wait a few months for the third to stream, but in all we really enjoyed them.
Now when I see advertisements for the American version with Rooney Mara, they just look so wrong. Noomi Rapace will forever be the face of Lisbeth Salander in my mind, the same with Michael Nyqvist as well.
I guess I'll have to see the American version to compare (as I did with "Brothers") but I recommend checking out the Swedish version if you haven't yet.
C., seeing the Swedish movies are very high on my list of things to do. If I can find them on cable, or in the library, or something, I'd see them as soon as this weekend.ReplyDelete
I also usually don't like remakes of good movies. Like, why did Gus van Zandt remake Psycho, especially if he's going to shoot it exactly the same, frame by frame? Dumb, and useless. Not an homage, either. But, in the case of DRAGON, I'm sure the American film company remade it because they knew that not too many Americans--or others throughout the world--would have seen the Swedish original. In this, they would be sadly correct. Americans, in general, do not watch foreign films.
I've heard that Noomi Rapace did a great job, and I'm sure she did. (She was okay in a simple role in Sherlock Holmes 2.) But give Rooney Mara a chance here: she was brilliant. I'll repeat, she owned this very difficult role.
I hope all is well. I received a computer camera for Xmas. You know what that means!!!
Good review! I have yet to check out the American version though, but I thought the Swedish one was quite good. The running time was a bit long, but so was the book :) I was very impressed with the structure of the plot as well as the performance by Noomi Rapace. She gave it her all! It may be difficult for you to be objective now having seen Mara's portrayal first. You should definitely watch it and compare the two. Hopefully I'll get a chance to check out the US version before it leaves the theater. For some reason I think Daniel Craig was more suited as Blomkvist than Michael Nyqvist. Just a hunch, I have.ReplyDelete
To the review above, I agree about the movie "Brothers", it was a weak remake!
Thanks, Diane. I'll keep an open mind about Rapace's performance, since her portrayal is well-regarded. I'll be looking for differences in their performances. I'm very interested in seeing it. I think the Blomkvist role is better suited for someone with a little dapper charisma and sleekness, which Craig has--as a current James Bond--and Nyqvist doesn't. Nyqvist just doesn't have the face or the charm for it, and I mean that in the kindest of all possible ways.ReplyDelete
You are far too kind. As a female, I will openly admit Nyqvist doesn't have the face or the charisma to pull off Blomkvist. While reading the book, I pictured him having more defined classically handsome features. He was a bit of a ladies man in the novel after all. Not to sound shallow, but I was a bit put off by the actor chosen for the foreign film. In both performance, charm, & physical resemblance. So I do agree Craig was the better choice. I mean that in the least superficial way possible.ReplyDeleteReplies
I saw Nyqvist in the last Mission Impossible movie and I just couldn't see him as Blomqvist. Then I saw Noomi Rapace in the latest Sherlock Holmes movie, and I could definitely see her as Lisbeth Salander. She has the sparkle in the eyes, and the intensity, for that role.Delete
Now that I've seen both the Swedish and American version of the film I have to say I agree with your review even more so! I honestly feel Fincher's version is the true adaption whereas the Swedish version was more of loosely based one. Aside from the plot changes and the different ending, we are blessed with one hell of a knock out performance by Rooney Mara! She possessed such a rawness yet vulnerability without changing who she was not once ever. I loved that. I agree with your thoughts on her mono-syllabic tone. She didn't budge not once with it, yet you could still get a sense she was opening her cocooned shell slightly with Blomquist and developing affection for him. What a phenomenal performance.ReplyDeleteReplies
I'm hoping the third film comes out soon. I've heard it's having problems because the screenplay will be nothing like the book. A good thing, since Salander is bedridden for most of the book, then unrealistically nimble. The movie should be better. I'm reading the fourth in the trilogy (Feel my disapproval?) now; so far it would be good only for fans of the trilogy. It wouldn't win over newbies.Delete


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Women's AMS Athletic T-shirt
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Trash Du Mer
category: > Indoor/Outdoor
THE coastline of Morocco is as pristine as a Sudanese refugee camp. The beaches look like overly decorated Christmas trees, with a variety of trash washed ashore. Take these bottles, jerry cans and empty tubes of toothpaste. Fresh picks from the beach at the surf spot K12, near Taghazout. My advice to the Moroccans: if you don't clean up your beaches, at least arrange the trash in an orderly fashion. Like I did below. It just looks more tidy.
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Results 1 to 4 of 4
Thread: Analyze Process
12-31-03, 01:08 #1Registered User
- Join Date
- Dec 2003
- Location
- India
- Posts
- 12
Unanswered: Analyze Process
Can anyone explain me the differences between the following types of analyze processes :-
1) analyze table abc estimate statistics sample 20 percent;
2) analyze table abc estimate statistics sample 20 percent for table for all indexes for all indexed columns;
3)analyze table abc validate structure;
4) analyze table abc compute statistics for table
for all indexes for all indexed columns;
5) analyze table abc compute statistics for table;
Which process would be the best if i need to analyze a 300GB database everyday .
Thanks in advance
Last edited by sbdash; 12-31-03 at 01:11.
12-31-03, 08:11 #2Drunkard
- Join Date
- Nov 2002
- Location
- Desk, slightly south of keyboard
- Posts
- 697
Hi Sbdash,
Only you can truly decide which method is best for your tables. Different tables can be analysed in different ways and some could be skipped altogether to gain the most optimal statistics.
You should also check out histograms on certain table columns - these can have a dramatic effect on performance, again only you can decide on which columns/tables these would be appropriate.
You should also consider moving to the DBMS_STATS package for statistics gathering etc.
Finally, it is unlikely that you would need to analyze the db every day. Unless the content (skew of data) of a table changes significantly each day you will gain no benefit.
I would suggest you read the Oracle tuning for performance guides, it's far too much to go into here.
BillPlease don't email me directly with questions. I've probably just got home from the pub and cannot guarantee the sanity of my answers. In fact, I can't believe I actually made it home.
12-31-03, 10:42 #3Registered User
- Join Date
- Sep 2003
- Location
- Milan, Italy
- Posts
- 130
For daily analyze, I would consider taking a look at the MONITORING option of the tables/indexes, which basically means that Oracle will flag the tables/indexes that were modified "more than 10%" so that you can re-analyze only them daily. Requires use of the dbms_stats package, that you would want to use anyway instead of the ANALYZE command which is somewhat "deprecated".
You can take a look at this question on Tom Kyte's site
which is exactly the same as your one.
12-31-03, 11:19 #4Registered User
Provided Answers: 1
- Join Date
- Aug 2003
- Location
- Where the Surf Meets the Turf @Del Mar, CA
- Posts
- 7,776
Over the last couple of days there has been much discussion on the ORACLE-L email list regarding the frequency when statistics should be collected.
A decent number of the resident Wizards now conclude that "frequent" collection of statistics; especially when done via regularly scheduled task, is no longer a "Best Practice".
FWIW - I tend to agree with them.
Happy New Year!
The official ORACLE-L FAQ: http://www.orafaq.net
You may also send the HELP command for other information (like subscribing) to email@example.com


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Sara Näse
You can contact me in English, Swedish or Finnish.
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Are You a Leader or a Follower, Virgo?
Leadership — based in natural power, courage and control, it's a quality most people respect and many desire to possess. After all, who wouldn't want to command a room as soon as they walked into it? We all want others to listen to us, to treat us with respect and to recognize our authority. But the world doesn't just need leaders. It also needs followers — people who take direction well and comply with the rules; people who manifest others' visions by carrying their ideas through to the finish. When it comes to getting great things accomplished, people rarely do it on their own. Instead, they join together in teams of leaders and followers — visionaries and those who bring those visions into reality. Thus, both leaders and followers are essential to progress. Are you a leader or a follower?
Virgo is the Sign of Service, which says it all. A natural follower, you're compassionate and considerate; you take direction well, and you feel most fulfilled when working selflessly for someone you love or respect. Instilled with a strong sense of duty, you don't shy away from hard work, especially not when it's something you believe in. Your frugal nature and practical perspective make it easy for you to bring others' grandiose ideas down to earth, where you can modify them into something feasible in the real world. Your tendency to be hard on yourself — harder than anyone else — makes you an excellent employee or team member, since you hold yourself to such high standards. Trustworthy and diligent, you always strive to do your best. You're an asset to any group or team of which you're a part.
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I am virgo and I wanna leader not only follower


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Jim and Adam sat down over a pint of internet to discuss the recently released imperial epic, Rome II. They had things on their mind.
Jim: So then. I am usually in the Total War apologist camp, but not this time. I am not sure if it's because I had a better experience with Shogun 2, or whether there's some kind of allergy due to over-exposure going on, but Rome II rubbed me up the wrong druid.
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NOTE: To place order using Credit Card, P-Card, or Purchase Order – please choose kit size above and click "Add to cart" button. You can place order online without creating an account.
Transfection Reagent for PANC-1 Cells (Non-endocrine Pancreatic Cancer)
- Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
- A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
Download in vitro PANC-1 transfection protocol: [PDF]
- Download PowerPoint presentation for PANC-1 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems PANC-1 Transfection Protocol: [PDF]
Download MSDS: [PDF]
PANC-1 Cell Line:
The PANC-1 cell line was established from the pancreatic duct disuse of a 56-year-old Caucasian male patient who suffered from epithelioid carcinoma. PANC is short for pancreas, referring to the Human pancreas, where cancer can be found. Research related to pancreatic cancer would be one use for this cell line, but it also makes for a good transfection host. Growth of these cells is inhibited by L-asparaginase.
PANC are used as an in vitro model of non-endocrine pancreatic cancer for tumorigenicity studies. These cells possess the type B phenotype for glucose-6-phosphate dehydrogenase G6PD and overexpress heregulin/human epidermal growth-factor receptor 2 (HER2/neu) oncogene, which is present in 60 to 70 percent of human pancreatic carcinomas, but are estrogen receptor (ER) negative. PANC cells have been show to be negative for MUC4 and positive for Smad4, a TGF beta signaling cascade protein inactivated in human gastrointestinal cancers. PANC can be induced to differentiate into hormone-producing islet-like clusters following stimulation by the FGF2 growth factor. The PANC-1 cell line has a doubling time of 52 hours and G6PD activity of the slow mobility of B type. Chromosome studies show a modal number of 63 with three distinct marker chromosomes and a small ring chromosome.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the PANC-1 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).
Figure 2. Protein expression of Cyclophilin B in PANC-1 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into PANC-1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems:
Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen's Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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Owners of new 2011 Chevrolet, Buick, Cadillac, and GMC vehicles mobile applications are now able to access their vehicle information and OnStar services from their smartphones. The mobile applications, which were made possible by General Motors Co. and OnStar, are dubbed myChevrolet and OnStar MyLink.
These are offered to Android, iPod and iPhone users as free downloads on android.com and apple.com. In a statement, GM said that these two applications' features fall into three categories: location-based services, vehicle-specific information, and OnStar connectivity.
It was in July that GM had announced plans for the new apps. Those who download the apps will get access to commonly used user manual information on vehicle functions such as instructions for radio-presets, and warning and indicator lights.
Users can use GPS enabled functions to get the location of their vehicle in a parking lot, and ask for roadside assistance or set a service appointment with the nearest Chevrolet dealership.
In a statement, Chris Perry, Chevrolet's vice president of marketing, said that this is a "great example" of how the company is focusing on smart technologies that respond to customers' needs.


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Monday, July 14, 2008
Youth Crime At Record Lows, While Violent Youth Crime Rises...
"Recent Statistics Canada figures show that while overall youth crime is down significantly in the past 15 years, youth violent crime has surged 30 per cent since 1991."
Interesting. My, what you can do with statistics, if not explained clearly. But, no-one ever looks at the causes behind the figures. Why has youth violent crime "surged"? Youth crime is DOWN, and SIGNIFICANTLY. Youth have more options nowadays, more jobs, etc. So, how do we explain the increase in PERCENTAGE of violent crimes? Let's simplify it for the slightly less mathematical minds out there: Imagine you have 10 apples, slightly bruised, with 3 of them really rotten (or 30%). You take out 5 apples, but not the really rotten ones. Now you have 5 apples left, with 3 of them really rotten - a whopping 3/5 or 60%!!!
Anyone who has studied Sociology and Psychology, finds it pretty easy to explain the seeming hike in violent crime. Violent criminals suffer from more extreme psychoses than non-violent offenders. They are likely to be affected in a more extreme way, and less likely to change their minds when the degree of punishment changes, or economic times change, or their social milieu changes. If you manage to reduce overall crime with effective measures for youth, you are less likely to reduce violent crime, as these criminals tend to be more "hardened" and suffering from more extreme psychoses.
The point about "degree of punishment" is also important in refuting Conservative arguments. Simply bringing in a death penalty, or harsher punishment, won't stop violent crime. As a matter of fact, offenders of that mindset may be MORE LIKELY to commit a violent act, if it means they will stay out of jail, or get out of the harsher punishment. The whole, "I'm never going to the chair, you'll need to take me in dead" argument. People who have psychoses, and cannot realistically negotiate wrong from right probably won't be likely to be curbed from action by another year or two in jail, and a life sentence will probably only make them commit violent acts to stay out.
The Cons will try to argue the same old ignorant argument, even laying on the "biblical" eye for an eye" routine. It will win a lot of hearts with the doorstep whisper campaign (hot-button politics always wins some easy votes). It will create a media buzz, as the networks try to outdo each other to display the most graphic look at violence on our streets. As able-minded, thoughtful people, WE need to stand up and paint this policy for what it really is: a last ditch Conservative attempt to curry favor with voters - in the only way they know - with the only issue they claim to own.
I wish the Cons good luck. If this is the only issue that casts them in a good light, and they're desperately reaching for it in this pre-election period, then we stand in pretty good stead.
1 comment:
- WesternGrit said...
What the media NEEDS to report about the figures is:
- Are the crime figures "per capita" (meaning, per 100,000 Canadians, etc.)
- Is the increase in violent crime in proportion to the increase in all crimes (that lends credence to my argument)?
Statistics can be manipulated - especially by people who control the media and industry... They have the means, and the opportunity. We have to be watchful.
Crime is at record lows across Canada, and with time, continues to go down. Reporting of crime, on the other hand, continues to rise. Sensationalism has replaced journalism in many quarters. Crime sells. Sensationalism moves papers. Other people's dirty laundry, if you will. The media is the most evil of tools, if twisted and corrupted by private or state interests. The news media, in particular, needs to be as independent as possible. The independence of the media - and in particular, individual journalists needs to be defended and protected, even if by law.
- 7:00 pm


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Reuters Market Eye - Bank of America-Merrill Lynch upgraded Bharti Airtel (BRTI.NS) and Idea Cellular (IDEA.NS) to 'buy' from 'neutral' citing the positive outcome of the 2G auction this week.
BoA-Merrill said the "deep" under-performance in both stocks over the past 12 months, potential for more favourable policy decisions in areas such as spectrum pricing, and prospect of "strong" fiscal 2014-15 earnings growth would drive Bharti and Idea.
An auction of 2G airwaves attracted less demand than targeted by the government, leading to lower prices that are giving a respite to the debt-ridden industry.
Bharti gains 3.5 percent, heading towards its fourth day of gains. Idea is up 1.5 percent, gaining for a second day.


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A power-packed laptop, the giant-sized GE70 2PE Apache Pro delivers the performance gamers demand.
MSI's bombastically titled GE70 2PE Apache Pro serves up serious gaming power in a 17.3in chassis. With a quad-core Core i7 processor alongside one of Nvidia's latest GTX 800 Series GPUs, the Apache Pro promises blindingly quick performance for $1,899.
It certainly looks like it means business. It's an imposing piece of kit, measuring almost 4cm thick and weighing a lumpen 3kg, and the chassis feels seriously tough. The metal wristrests hardly flex during typing, and the metal extends all around the keyboard, giving the laptop's entire base reassuring solidity.
One of the Apache Pro's key assets is its dedicated GeForce GTX 860M graphics chip, which sits in the middle of Nvidia's new 800 Series range, and boasts the new, power-efficient Maxwell microarchitecture. In contrast with the previous Kepler architecture, the Maxwell generation distributes its CUDA cores into several separate blocks, each of which can be dynamically toggled on and off to minimise power usage. As a result, Nvidia claims the new Maxwell GPUs deliver twice the performance per watt of their Kepler predecessors.
The GeForce GTX 860M finds itself in good company. Working alongside is a quad-core Core i7-4700HQ CPU supported by 16GB of DDR3 RAM, a pair of 128GB SSDs in a RAID0 array and a 1TB HDD. The pair of SSDs ensures lightning-quick boot-up and application-load times, and achieved sequential read and write speeds of 836MB/sec and 505MB/sec in the AS SSD benchmark. It came as no surprise to see the Apache Pro breeze through our suite of Real World Benchmarks with an overall score of 0.97.
Gaming performance is equally impressive. The GeForce GTX 860M completed our Crysis benchmark – run at 1,920 x 1,080 resolution and High detail settings – at a silky-smooth average frame rate of 65fps. Even with Crysis cranked to Very High detail settings, the Apache Pro kept up a playable average of 42fps.
Nvidia's new Battery Boost feature helps eke the most from the MSI's battery, however. This allows the user to cap the maximum frame rate between 20fps and 50fps in 5fps increments and, once this is set, the GPU's clock speeds and power requirements are adjusted on the fly to minimise power consumption. To put this to the test, we repeated our Crysis benchmark at 1,280 x 720 resolution and Very High detail settings. With the frame rate capped at 50fps, the MSI lasted 53mins, and with the frame rate set to 25fps it lasted 1hr 5mins – a 23% increase in battery life.
The MSI's display is a high point. The matte finish of the 17.3in, Full HD panel all but banishes distracting screen reflections and, thanks to the 1,101:1 contrast ratio, images are eye-poppingly dynamic.
Ergonomically, MSI has kept standards high. The SteelSeries Scrabble-tile keyboard combines a spacious layout with great-feeling keys, and the multicoloured backlighting comes in handy when the lights go down. The laggy-feeling touchpad is a shame, but it's bearable for short periods and, helpfully, is disabled when you plug in a USB mouse.
Connectivity includes two USB 3 ports, a pair of 3.5mm audio jacks, an HDMI port and an SD card reader. Along the right there are two USB 2 ports, a DVD writer, and Gigabit Ethernet and VGA ports. Wireless networking ticks all the boxes, too: there's dual-band 802.11ac Wi-Fi and Bluetooth 4.
Like all gaming laptops, the MSI is expensive. It's also given a run for its money by the considerably more compact Gigabyte P34G v2. However, if you're on the hunt for a big-screened gaming laptop the MSI GE70 2PE Apache Pro comes heartily recommended.


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Mathematical Programming
A Publication of the Mathematical Optimization Society
Mathematical Programming publishes original articles dealing with every aspect of mathematical optimization; that is, everything of direct or indirect use concerning the problem of optimizing a function of many variables, often subject to a set of constraints. This involves theoretical and computational issues as well as application studies. Included, along with the standard topics of linear, nonlinear, integer, conic, stochastic and combinatorial optimization, are techniques for formulating and applying mathematical programming models, convex, nonsmooth and variational analysis, the theory of polyhedra, variational inequalities, and control and game theory viewed from the perspective of mathematical programming. The editorial boards are particularly interested in novel applications of mathematical programming and interfaces with engineering, economics, and computer science. Articles primarily concerned with computational issues such as implementation and testing should in general be submitted to Mathematical Programming Computation.
Mathematical Programming consists of two series. Series A publishes original research articles, expositions and surveys, and reports on computational experimentation and new or innovative practical applications as well as short communications dealing with the above. Issues of Series B each focus on a single subject of current interest to the mathematical programming community. Each issue of Series B has one or more guest editors, who need not be members of the editorial board. An issue may be a collection of original articles, a single research monograph or a selection of papers from a conference.
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A study of the difference-of-convex approach for solving linear programs with complementarity constraints
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The Films of Ken Russell
Movie - Bucket Of Blood (Roger Corman, 1959)
American comedy horror film staring Dick Miller as waiter-turns-artist Walter Paisley ... "The ultimate art movie." (Jen Ray)
Henry Kenneth Alfred Russell (3 July 1927 – 27 November 2011) was an English film director. He was known for his pioneering work in television and film and for his controversial style. He was criticised as being over-obsessed with sexuality and the church. His subject matter was often about famous composers, or based on other works of art which he adapts loosely. Russell began directing for the BBC, where he did creative adaptations of composers' lives which were unusual for the time. He also directed many feature films independently and for studios.
He is best known for his Oscar-winning film Women in Love (1969), The Devils (1971), The Who's Tommy (1975), and the science fiction film Altered States (1980). Classical musicians and conductors held him in high regard for his story-driven biopics of various composers, most famously Elgar, Delius, Liszt and Mahler.
One noted admirer, the British film critic Mark Kermode, attempting to sum up the director's achievement, called Russell; "somebody who proved that British cinema didn't have to be aboutkitchen-sink realism—it could be every bit as flamboyant as Fellini. He now makes very strange experimental films like Lion's Mouth and Revenge of the Elephant Man, and they are as edgy and out there as the work he made in the 1970s."
Amelia and the Angel (1957)
Shelagh Delaney's Salford (1960)
London Moods (1961)
Movie - Bucket Of Blood (Roger Corman, 1959)
American comedy horror film staring Dick Miller as waiter-turns-artist Walter Paisley ... "The ultimate art movie." (Jen Ray)


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Checkpoint Systems
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Checkpoint Systems is a global leader in merchandise availaibility solutions. Checkpoint partners with retailers and their suppliers to reduce shrink, improve shelf availability and leverage real-time data to achieve operational excellence. Checkpoint solutions are built upon 40 years of RF technology expertise, diverse shrink management offerings, a broad portfolio of apparel labeling solutions, market-leading RFID applications, innovative high-theft solutions and its Web-based Check-Net data management platform. As a result, Checkpoint customers enjoy increased sales and profits by improving supply-chain efficiencies, by facilitating on-demand label printing and by providing a secure open-merchandising environment enhancing the consumer's shopping experience.
Checkpoint's RFID solutions portfolio encompasses:
- RFID tags
- RFID hardware
- RFID software
- RFID implementation and field services
Checkpoint's advanced solutions are ushering in the age of digital retailing.
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Checkpoint Systems Expands Health and Beauty RFID Label Portfolio For Improved Merchandise Visibility
THOROFARE, NJ – April 28, 2016 – Checkpoint Systems, Inc. (NYSE:CKP), a leading global supplier of merchandise availability solutions for the retail industry, today announced that it has expanded its portfolio of RFID labels for health and beauty products with its new Compass RFID label for improved inventory management.
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Zebra Technologies' New RFD8500 Sled Brings Enterprise-Class RFID and Barcode Scanning to Android and iOS Mobile Devices
LINCOLNSHIRE, Ill. – April 15, 2015 – Zebra Technologies Corporation (NASDAQ: ZBRA), a global leader in products and services that provide real-time visibility into organizations' assets, people and transactions, today announced its first multi-operating system RFID sled designed to easily add high-performance RFID and 1D/2D bar code scanning to Android™ and iOS™ mobile devices.
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Brady to Exhibit at the 2015 RFID Journal LIVE! Conference and Exhibition
Company will showcase its RFID part marking and trace
MILWAUKEE, Wis. (April 15, 2015) — Brady (NYSE:BRC), a global leader in industrial and safety printing systems and solutions, today announced it will showcase its RFID part marking and traceability solutions at the 2015 RFID Journal LIVE! Conference and Exhibition. The conference will take place April 15-17, 2015, at the San Diego Convention Center in San Diego, California. Brady will be exhibiting at booth #248.
Checkpoint Systems Introduces New High-Speed, High-Quality RFID Tag and Label Production Capabilities
Speed, Quality, Efficiency and Service Improved for Retailers With EVO, Check-Net Mobile and New Printshop in Asia
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Checkpoint Systems' New RFID Labels for Health/Beauty/Cosmetics
Categories Based Upon Latest Impinj Chip
San Diego – April 14, 2015 – Checkpoint Systems, Inc. (NYSE:CKP), a leading global supplier of merchandise availability solutions for the retail industry, today announced at RFID Journal Live its new Slim and Whisper RFID labels for Health/Beauty/Cosmetics Categories. These categories have specific inventory management challenges based on their small size, SKU complexity and densely-packed merchandising displays.
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1st Choice Security Solutions is an OEM Manufacturer and Distributor of ultra-long-range, hands-free RFID readers, tags, accessories and software for vehicle identification, asset loss prevention and real-time inventory, visitor and employee monitoring, and emergency evacuation and personnel muster accountability. The company also produces real-time location systems (RTLS), wireless wiegand access control for gates and doors, GPS tracking and inmate house arrest systems.
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Developer of novel thin film transistor (TFT) based technologies enabling lower power, lower cost, and higher performance electronics. Printable RFID tags is one target application. Novel unipolar logic circuit technology enables lower power than CMOS for TFT-based circuits. Other IP related to monolithic 3D chips for memory and logic.
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3DT Solutions offers customizable RF and RFID technologies, combined with secure and encrypted data storage, to offer clients a flexible range of solutions.
3in1GroupUSA with it's factory in Asia has developed a unique technology to enable the RFID inlay to be transferred to the surface of the fabric and paper by hear and pressure. The inlay remains soft and super thin after transference just like printing directly onto the surface of the material. Our RFID inlays can be applied as stickers onto hangtags or sandwich in between paper products or heat transferred onto back of labels or onto actual garments in seams or labels.
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Supporting young people towards a
healthy and pain free lifestyle
Your child is the centre of our attention
is dedicated to joint & muscle (musculo-skeletal) problems in younger people. Lead by our principal therapist Sid Ahamed, who is one of the U.K's leading lecturers in paediatric orthopaedic physiotherapy.
We cover the full range of childhood injury
from joints and soft tissues to spinal problems.
In addition
see children with neuro-muscular and movement development or control issues to help them develop normal movement, restore mobility, balance and co-ordination.
For Orthopaedic Investigation
we have referral links with the region's leading specialists.
Suitable treatment ages
Typically we see children from the age of 5-6 yrs old.
But younger children may also be appropriate for treatment.
Contact us to see if treatment is suitable for younger ages.
Treatment period
Young people rarely need lengthy courses of treatment.
Normally appropriate assessment, professional advice and guidance will address a young person's issues.
Non specific issues
These include poor co-ordination, balance and movement control.
In these cases we offer specialist programs ranging from supervised exercise, through personal training to DMT a new form of therapeutic exercise.
Also see our kids club health and fitness page for more details
Dedicated to the needs of young athletes
Children and adolescents suffer from a unique set of injuries that adults by definition cannot have i.e. injuries to the growth tissues within the developing skeleton. Growth tissues such as growth plates (physeal plates ) , or cartilaginous tendon insertions ( apophyses or traction epiphyses ) do not exist in the adult skeleton having matured and ossified.
Our physiotherapists have the specialist post-graduate training for this area of practice
Children and adolescents suffer from a unique set of injuries that adults by definition cannot have i.e. injuries to the growth tissues within the developing skeleton. Growth tissues such as growth plates (physeal plates ) , or cartilaginous tendon insertions ( apophyses or traction epiphyses ) do not exist in the adult skeleton having matured and ossified.
Our physiotherapists have the specialist post-graduate training for this area of practice
- Balance and co-ordination problems
- Limitation of range of movement
- Muscular weakness and slow development
- Hypermobility syndromes (over-flexible joints)
- Assessment for orthotics and orthopaedic supports
- Torticollis (painful or stiff neck)
- Rehabilitation following fractures, dislocations & surgery
Paediatric Teaching
Sid has lectured on paediatric orthopaedics, sports injury and musculo-skeletal conditions to the physiotherapy profession for over 20 years. He has presented at major national and international conferences and taught training courses at local, regional and national level. He is a published author and has written papers on a variety of paediatric issues.
Our patients tell us...
I took Lucy to Splash World yesterday and it was the first time ever that she's been able to stand without holding hands paddling. She went in up to her knees and it was so lovely to see. I just wanted to say a big thank you! With your help with physio and the programme you put in place Lucy was able to enjoy the beach mostly unaided for the first time. There's such a hugh difference in Lucy after doing this last session with you. So thanks again from Lucy and myself for all your time and hard work, it's really appreciated.
We approached several private physios and were particularly impressed with Nadia right from our initial assessment session. He took the time and effort to clearly explain how Nadia's condition would affect her ability to control her movement, and how this linked in with the approach that he would use to treat her. He has taught us how to integrate physiotherapy into everyday activities, and as a result we have seen great progress in Nadia's development, and also her attitude to physiotherapy in general. We have seen the benefits of the work in Nadia's movement, and have happily recommended the clinic to friends whose children require help with physical development.
I struggled to get Charles to co-operate with various private physios and therefore relied on his once-a-month NHS appointments. Finally I came across Physio4Kids following a recommendation. I've never been so pleased with the help, support and knowledge of a physiotherapist. From the 1st appointment Charlie has engaged and responded very well with therapy. I have no hesitation in recommending their service to any families needing flexible, reliable and most of all professional care for there child/children. Many thanks for your continued support.