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Where can I buy a thirties style front door?(9 Posts)
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My relationship with you has nothing to do with you.
This is an understanding that I recently learned while having a conversation one evening with two very close friends of mine, Aiden and Tom.
At first, this statement seems very contradictory and even selfish, but I assure you that its the most selfless or self-ful that you could be.
To be affected by another being is to give that being power of our emotions and directions of our thoughts.
Being affected by another being is to have the understanding that this person has something for me to learn and there is a lesson here that is being taught - that person will only be free from teaching you this lesson once you learn it, they are being as much captive by this situation as you are. It is not their fault, or their doing.
The same applies for most types of relationships whether it is business, personal, partners, love or family and friends.
People can transition from various relationships with us in our lifetime and we need to determine what space they will be occupying in our lives – such as if a love partner were to transition out of that relationship, would they be a close friend, an acquaintance or simply a lesson learned.
We also need to identify why our current relationships exist and why we have them – what need and energy exchange is happening for it to be alive.
For example if I were to use the relationship of a love partner – are we indeed in this relationship for our need of love sharing, the giving and taking of love, and are we adequately receiving love in return – if we are not, its a relationship of giving – which ultimately drains us and will not last – if we get as much as we give, its a relationship of gain, these are made to last.
If we identify this need of love sharing – of energy exchange – then we become fully capable of this exchange with this other person without any obligations or reservations. It is also vital to understand and agree upon exactly what love is for each of us within a relationship so that we understand what each others capacity and understanding of the giving and receiving is.
Once this understanding is reached – we then come to the understanding that My relationship with another person, has nothing to do with them – its to do with me.
Having this understanding in a relationship could bring one to the question of: "Do you love me or do you simply love loving me?"
We may not be completely capable of loving everyone, or at least we do not love everyone to noticeable and strong degrees.
Love then is about our understanding and ability to love and the other beings understanding and ability and compatibility to be loved by us.
A life partner then is a perfect permutation of material (or spiritual) nature that is designed specifically to receive and return our love, otherwise known as soul-mates. This sharing within a relationship creates a factor that is conducive for happiness - but happiness needs to be found and awakened in our hearts – from within.
If we can find that place of peace within our hearts, that balance with our relationships with others that do not completely depend on other people – we would have found contentment with equilibrium.

subset: commoncrawl


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- F. M. Hons,
- R. F. Moresco,
- R. P. Wiedenfeld and
- J. T. Cothren2
Limited information is available concerning nutrient requirements of high-energy sorghums (HES) (Sorghum bicolor L., Moench). The purpose of this study was to compare a HES, an intermediate grain cultivar (IGC), and a conventional grain cultivar (CGC) for grain and biomass (stover) yield, responses to applied N and P, and effects on nutrient partitioning and removal. Field experiments were conducted in 1983 and 1984 on Ships clay (Udic Chromustert) near College Station, TX. Nitrogen (0, 84,168 kg N ha−1) and P (0, 15, 30 kg P ha−1) were factorially applied to the three cultivars. Soil nutrient availability decreased after only one year of total dry matter harvesting and was reflected in greater responses to applied N and lower tissue nutrient concentrations and removals the second year. Cultivar and N influenced grain and biomass yields, while applied P had little effect on these parameters. The CGC and IGC produced more grain, but less biomass, than HES. The CGC removed the least nutrients in biomass and the most in grain, while removals by HES components were reversed. Although HES did yield more total dry matter, total crop N and P removals were similiar for all cultivars. HES did remove greater total quantities of all other nutrients. Applied N increased concentrations and uptake of most nutrients in grain and biomass.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
Copyright © . .


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Over 100 kids parade on MCLB
Sat, 09 Apr 2011 02:05:44 GMT —
While parents are stationed miles away, military children can sometimes feel a void. Family, friends, and mentors often times try to fill it.
"We just try to console them and love on them and let them know how much we care," said Marine Corps Logistics Base Child Development Center Director Dorothy Bryant.
On Friday at 3:00 p.m., the Child Development Center (CDC) threw a parade in honor of the month of the military child. There were no floats, horses, or throws in the parade. Parents and teachers pushed toddlers along in strollers and baby buggies.
Children waved and marched to the sounds coming from the band, while teachers belted out chants.
After the parade, children had the option to eat, jump in bounce houses, or have their faces painted.
While some parents weren't able to make the event, many were in attendance. One father remembered the feeling of his friends trying to cope while away from their families.
"It was tough on them. I've been very fortunate not to go back," said Marine Staff Sergeant Jacob Perry.
For a job that's hard to explain to children, supporters simply display their thanks.
"We really appreciate our military families. Without them we wouldn't be here," said CDC Director Dorothy Bryant.


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Located a short drive from downtown Atlanta, and conveniently situated just off Interstate 75, this hotel offers exceptional accommodations, state-of-the-art amenities and is close to many corporate offices. Doubletree Hotel Atlanta Northwest/Marietta places guests close to many major businesses. IBM, Coca-Cola Enterprises and Lockheed Martin are all nearby. The sites of downtown Atlanta, including the CNN Center and Zoo Atlanta are also close by. Guests at the Marietta Doubletree can enjoy a relaxing sleep on the hotel's exclusive Sweet Dreams beds, watch an in-room movie on flat-screen TVs or work out in the state-of-the-art fitness center. The hotel also offers an on-site restaurant as well as a wine bar, featuring an extensive wine list.
Call 1-800-774-1500 for cancellation policy inside the US and Canada; 1-972-770-6136 outside the US.
2055 South Park PlaceCP:30339-2014,Atlanta,Estados Unidos
Teléfono:No especificadoFax:1-770-272-9443
We travel back and forth from Columbus to central Florida on a regular basis; finding a place to stay overnight has been hit or miss.2012-11-12 - hotels.com
Great Service,very clean and spacious rooms.2012-11-06 - hotels.com
Love the beds.2012-11-03 - hotels.com
Great Amenities2012-10-25 - hotels.com
Free internet if you are a Hilton member, very clean quiet room, convienent Northern Atlanta, easy to drive downtown, local area is slightly rundown, be careful, don't leave valuables exposed in car2012-10-16 - hotels.com
Hotel is great value2012-10-16 - hotels.com
However, these are small complaints as the room service was excellent, and the location of the hotel was perfect for our needs.2012-10-15 - hotels.com
The bed was very uncomfortable, I could feel every spring in the mattress.2012-10-02 - hotels.com
My only complaint is that there wasn't a refrigerator in the room.2012-08-27 - hotels.com
lavatory sink was slow in draining; room key card didn't work last night of my stay so had go back to front desk to have it reactivated; the ladies at the front desk didn't seem very friendly...2012-08-25 - hotels.com
I was a bit thrown off to see there was no refrigerator or microwave in the hotel room, but my family staying at another hotel said it was the same in there's too.2012-08-11 - hotels.com
The front desk staff was rude and not helpful at all.2012-07-17 - hotels.com
It would have been nice if there was a mini fridge.2012-07-10 - hotels.com
They have both an indoor and outdoor pool both were small and a hot tub.2012-07-08 - hotels.com
You get bad beds or something smells.2012-07-08 - hotels.com
Very unfriendly staff.2012-07-06 - hotels.com


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Vanilla Night & Day are known for creating high quality, luxurious nightwear and loungewear. Their designs are exclusively made in Poland where they use quality fabrics and lace to create an array of new colours and styles each season. New fabrics are being introduced with Satin and Cotton being the most popular. They explore new styles ready for any occasion including Christmas and Birthdays and always have something special for your Wedding day.
You will find the collections are based on comfort, elegance and pure indulgence with dressing gowns that co-ordinate with a selection of pyjamas, chemises and nightdresses.
Make sure you keep your eyes peeled for next seasons additions of gorgeous new colours and flattering styles. Discover them here at Knicker Locker.
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Baadasssss! Trailer (2004)
"A Father - A Son - A Revolution" 20 January 2004 Drama 108 mins
Director Mario Van Peebles chronicles the complicated production of his father Melvin's classic 1971 film, "Sweet Sweetback's Baadasssss Song." Playing his father in the film, Van Peebles offers an unapologetic account of Melvin's brash and sometimes deceptive conduct on the set of the film, including questionable antics like writing bad checks, tricking a local fire department and allowing his son, Mario, to shoot racy sex scenes at the age of 11.
Watch the official Baadasssss! 2004 trailer in HD below or find more Baadasssss! videos on Vidimovie.


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Sen. Bernie Sanders lashed out Wednesday at widespread use of offshore tax havens by U.S. companies, and the liberal independent targeted a group that represents CEOs of big corporations and wants corporate taxes lowered.
Sanders, top opposition member on the Senate Budget Committee, released a report decrying what he called "legalized tax fraud." It showed that 111 of the 201 member companies of the Business Roundtable are sheltering more than $1 trillion in profits overseas, where they are not subject to U.S. taxes.
Using the Cayman Islands, Bermuda and other tax havens, these companies have saved more than $280 billion in tax liabilities, Sanders concluded in the report.
The senator from Vermont delivered his broadside a day before a media event the Business Roundtable scheduled at its Washington office. The group was expected to call again for a rewrite of the tax code including a lower corporate income tax rate.


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In 2003, Pat Tillman, serving in the US Army, hastily wrote a "just in case" letter to his wife, Marie. When he returned on leave before his departure to Afghanistan, he placed the letter on top of their bedroom dresser. For months it sat there, sealed and ever-present, like a black hole through which Marie knew her stable life would be pulled if she ever had reason to open it. Then, in April 2004, Marie's worst nightmare came true. In the days following his death, it was Pat's letter that kept her going, and more than that, it was his words that would help her learn to navigate a world she could no longer share with her husband.
In The Letter, Marie's talks for the first time about her journey to remake her life after Pat's death. In it, she recalls meeting and falling in love with Pat when they were kids, his harrowing decision to join the army after 9/11, and the devastating day when she learned he'd been killed. She describes how she withdrew from the public spotlight to grieve, learning along the way the value of solitude, self-awareness, and integrity in the healing process. And, finally, Marie recounts her work to rebuild her life, including founding The Pat Tillman Foundation, an organization established to carry forth Pat's legacy of leadership, and her decision to step back into the public eye in order to inspire people to live with meaning and purpose.
Filled with the lessons Marie learned and the wisdom she gained since Pat's death, The Letter is both a heartrending love story and an inspiring tale for anyone, young or old, whose life has taken an unexpected hard turn - and who struggles to get back on the right path.
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Definitely Space Mountain It would be the scariest there, but I don't think of Disney as having really scary rides, definitely exhilarating and fabulous but not that scary.
Oh yeah, there was the time I went with my best bud (whose husband works there) and we got stuck on Splash Mountain for literally 15 minutes. It wouldn't have been so bad but we were stuck in the up position so our necks were killing us. Then when the guy came out of the side of the top of the mountain (where we were stuck) and told us the ride was shut down because THE CAMERA WASN'T WORKING I am very our spoken (understatement of a lifetime) and replied, ARE YOU KIDDING ME? (I didn't cuss there were kids)
The ironic part of the whole thing was even though she had been there hundreds of times (I'm not exaggerating) it was her first time on the ride and it took me an hour to get her to do it. We laughed when we got down, waited a little while and rode it again :))
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Who runs the books?
In my home I take care of the checkbook, bills, budgets and so on. How about you?
Add your quick reply below:
You must be a member to reply to this post.
Replies (1-7):
1-7 of 7 replies
My husband is the one that is in charge of the bills. I am lousy at book keeping.
by Bronze Member on Feb. 24, 2014 at 8:48 PM
My husband pays all the bills but I keep track of the budget.
We have everything set up online, so it is pretty easy to share the duty and keep up with things, even though his end is done while he's at work and I do it from home.
Claire Wait
My blog: TheUnderToad.com.
by Member on Feb. 25, 2014 at 6:02 AM
I take care of if all. He earns the money and I budget it and pay the bills.
Posted via mobile.cafemom.com
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We know the sun rises and sets as does the moon. There are rainbows, but does anyone know what a moonbow is? Do you know where you can see one?
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A moonbow (lunar rainbow) forms when the light reflected off the surface of the Moon is diffracted when interacting with water droplets suspended in a rain cloud or the mist of a waterfall. Unlike the colorful rainbows produced by the sun´s light, moonbows are created by the sun´s light that is reflected off the surface of the moon. You can see a moonbow on clear full moon night close to the banks of a waterfall or through the mist of a light rainy night. Read; "https://hubpages.com/education/How-a-Moonbow-is-fo..."
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With First Tutors: Music you can locate a Ripley mandolin tutor. Mandolin lessons are a great way to start a fun new hobby, pick up a new talent and gain confidence with mandolin. Please use our site to search for Ripley mandolin tutors and determine which best matches your requirements so you can start your mandolin lessons in Ripley straight away!
This is the best way to contact Ripley mandolin tutors in the UK, helping you discover Ripley mandolin tuition close to home and Ripley teachers to help with any instrument, for example, piano, violin or guitar. We can help you find Ripley mandolin tuition or a one-to-one Ripley mandolin teacher to prepare for mandolin exams and improve your mandolin skills! We order Ripley mandolin teachers local to you by their tuition prices and distance from you.
If you are a mandolin tutor wishing to offer mandolin tuition services in Ripley or anywhere else across the UK, please register with First Tutors: Music or learn about us here. Becoming a Ripley mandolin teacher is a wonderful way to re-affirm your own skills through providing mandolin lessons while assisting someone else, all in Ripley!


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Shanghai, Guangdong China (PressExposure) June 02, 2007 -- EDraw Office Viewer Component contains a standard ActiveX control that acts as an ActiveX document container for hosting Office documents (including Microsoft Word, Microsoft Excel, Microsoft PowerPoint, Microsoft Project, and Microsoft Visio documents) in a custom form or Web page. The control is lightweight and flexible, and gives developers new possibilities for using Office in a custom solution.
The control is designed to handle specific issues that make using ActiveX documents from a non-top-level host window difficult, and serves as a starting place for constructing your own embedded object file viewer or editor as an ActiveX control.
It is a great solution for companies wishing to display read-only Word document data and Excel sheet to their employees while restricting modifications to the underlying data. You can play the powerpoint file in the same window. You can also display password protected Word documents without supplying passwords to your users - keep your confidential data safe and secure! With the office ocx it is easy to upload or download file between server and client via HTTP/FTP.
This component shows allows developers to embed Office files for in-place editing and viewing. It can be easily integrated into applications written in languages that support ActiveX control such as Visual C++, Microsoft Internet Explorer, Visual Basic, Delphi, C++ Builder and .Net languages to view Office files inside their main solution, and give them programmatic control over the document while it is embedded.


text: We are small and passionate team willing to create helpful software applications and tools. Our goal is to create and provide software which will help people enjoy the multimedia world. We believe that DeviceOnline is simple enough, user friendly and yet powerful tool for multimedia sharing and streaming. We are happy to improve DeviceOnline every day and watching it how evolves to a better app. If you want to get in touch with us please do it from here.


text: Within Edward Snowden's recent revelations is a warning that the random number generation hardware used in Intel computers was compromised by the NSA. This project serves as both a tactical response to state overreach, and as an entry point into a philosophical conversation about the nature of random. I posit a continuum of potential for 'randomness' from machines to biological creatures. In BRNG, I track the unpredictable movements of cilliates to build high entropy random numbers. The microbes were harvested in Bluffdale, Utah, near the massive NSA Data Center.


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Mass Media and Public Opinion. Chapter 8. The Formation of Public Opinion. Public Opinion: it is a complex collection of the opinions of many different people. Usually refers to the attitudes held by a significant number of people on matters of government and politics.
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Chapter 8


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In an interview with the Associated Press on Thursday, Oprah admitted that she was in a state of disbelief after she learned that Donald Trump had won the 2016 United States presidential election.
RELATED: EXCLUSIVE: Gayle King Talks Oprah's Favourite Things 2016 — Find Out Oprah's Favourite!
Oprah, who had been a longtime and vocal supporter of Hillary Clinton, said that, after watching President Barack Obama's meeting with President-Elect Trump at the White House on Thursday, she found some comfort and that "hope is still alive."
"I could sense, maybe I'm wrong, but I could sense from Donald Trump's body language even when he came out for the acceptance speech, that brotha has been humbled by this whole thing. I think it's a humbling process that now you literally have the weight of the world on your shoulders," Oprah said.
RELATED: Oprah Reveals Cover Of New Cookbook: 'It's My Life Story — Told Through Food'
Oprah added that she does not feel like Trump's win was a refusal of Obama's eight years in office and credits his victory to his millions of supporters who felt like their voices were finally being heard.


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The Book Report Network
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Feb 18, 2005
February 18, 2005
Bookreporter.com's Suspense/Thriller Author Spotlight Team (Carol Fitzgerald, Joe Hartlaub and Wiley Saichek) interviewed Michele Martinez, author of MOST WANTED. Martinez discusses her transition from prosecutor to crime fiction writer, the significance of her protagonist's Hispanic roots, and the challenging and rewarding aspects of writing her first novel.
Feb 4, 2005
February 4, 2005
Bookreporter.com Co-Founder Carol Fitzgerald and contributing writer Shannon McKenna interview debut novelist Miranda Beverly-Whittemore, author of THE EFFECTS OF LIGHT. Beverly-Whittemore talks about her inspiration for writing the book and addresses a central aspect of the novel that is likely to be a hot button issue with some readers. She also discusses the significance of the title, how her favorite writers have influenced her, and what her next project will be.
Feb 1, 2005
February 2005
In this interview Daniel Silva talks about his interest in the Arab-Israeli conflict, shares why he chose this moment to write about terrorism and sheds light on his character Gabriel Allon, as well as revealing some of his personal reflections on researching PRINCE OF FIRE.
Jan 7, 2005
January 7, 2005
Bookreporter.com's contributing writer Shannon McKenna, along with Carol Fitzgerald and Wiley Saichek, interviewed Sarah Graves, author of TOOL & DIE, the eighth installment in her "Home Repair is Homicide" mystery series. Graves talks about the rewarding and challenging aspects of writing recurring characters, her inclusion of home repair hints throughout her novels, and her fairly extensive background in various fix-it projects.
Dec 17, 2004
December 17, 2004
Bookreporter.com reviewer Bronwyn Miller interviewed Fannie Flag, author of numerous New York Times bestsellers including FRIED GREEN TOMATOES AT THE WHISTLE STOP CAFE and STANDING IN THE RAINBOW. Flagg talks about her choice of setting for her latest novel, A REDBIRD CHRISTMAS, and the development of her characters, many of whom are strong, irrepressible women. She also explains how being dyslexic and having A.D.D. impacts her writing routine and why she thinks Southerners have such a flair for storytelling.
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Results 1 to 4 of 4
Thread: Hi I have a problem
01-29-09, 06:42 #1Registered User
- Join Date
- Jan 2009
- Location
- Greece , Thessaloniki
- Posts
- 1
Hi I have a problem
Hi there what are you doing ????? Well I have a problem and I hope you will help me . I delete some files of my NOD32(Tools->Logs & Quarantine) by mistake , and it doesnt allow me now to enter the hard disk or internet explorer What have I to do ????? plZ help me Thnks
01-29-09, 09:01 #2Registered User
- Join Date
- Mar 2008
- Posts
- 89
Not at all database-related... unless of course you see the file system as a form of database.
Generally we would need more information. Do you happen to know what type of files you deleted? What exactly are you trying to do that doesn't work anymore? Do you get error messages? Did you try a restart?
Without knowing all that, I would say the NOD32 antivirus should be disabled as it seems to block actions it shouldn't. Then the program's security level might need adjusting, if it is accidentally set too high for some reason.
And well... give us more info to feed from please."My brain is just no good at being a relational Database - my relations suck real bad!"
01-29-09, 21:06 #3Registered User
- Join Date
- Jan 2009
- Posts
- 4
You can try to disabling NOD 32 process in task manager or reinstalling NOD32. Hope this will help.
02-06-09, 06:38 #4Registered User
Originally Posted by brandseo
- Join Date
- Mar 2008
- Posts
- 89
But disabling NOD32 is the first step.
However we haven't received any answer to our suggestions. I wonder if the problem was resolved or not."My brain is just no good at being a relational Database - my relations suck real bad!"


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Why Libya Could Be a Buying Opportunity
Chaos in North Africa this week sparked a surge in oil prices—and prompted traders to pile out of stocks.
Previous episodes of geopolitical uncertainty suggest investors might want to stay put.
The Standard & Poor's 500-stock index dropped 1.7% this week, the largest weekly selloff since November, as violent protests in Libya sent oil prices rising more than 9%. The fear is that the political revolutions that have engulfed...


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Aamir Khan on Salman Khan's rape remark: Unfortunate and insensitive
Aamir Khan is the latest celebrity from Bollywood to weigh in on Salman Khan's controversial rape analogy, saying it is unfortunate and insensitive if he made those comments.
Aamir Khan at the poster launch of 'Dangal'. Pic/Yogen Shah
"I was not present... Going by media reports, I feel what he said is unfortunate and insensitive," Aamir Khan told reporters in Mumbai.
When asked if he would give any advice to Salman on this, Aamir said, "Who am I to give advice?" The 'Dangal' star, 51, was speaking at the poster launch of the film.
Aamir said he is yet to speak to Salman on the issue. Salman had landed himself in soup when he compared himself to a raped woman while talking about the the physically gruelling shoot of 'Sultan'.
The actor has stayed mum on the controversy despite demands for an apology by womens' groups.


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Jenna Ushkowitz and her boyfriend Michael Trevino officially split after three years of dating. The breakup was announced when the 28-year-old "Glee" actress attended the "Normal Heart" premiere in Beverly Hills on May 19, 2014. "No, we're not together anymore," she replied when asked if the couple were still an item at the event. "That's OK. No worries. We're wonderful." No word on what caused the separation as well as when the actual breakup took place.
Jenna Ushkowitz and her boyfriend Michael Trevino, who joined actress Emma Roberts in a sexy, risque photo shoot with celebrity photographer Tyler Shields, started dating back in 2011. They made their first public appearance together at the SAG Awards in the same year. They have remained private, rarely speaking about their relationship but sharing a few pictures together on social media.
Back in January, Jenna Ushkowitz posted a throwback picture of the couple from the 2011 SAG Awards in honor of her ex-boyfriend Michael Trevino's birthday. "Happy birthday to my best friend, the one who has taught me how to laugh and love more and more everyday. Congrats on another year around the sun," the beautiful star gushed.
Jenna Ushkowitz and her boyfriend Michael Trevino split after dating for three years. The breakup was announced by the "Glee" star when she showed up at the premiere of Ryan Murphy's HBO film "The Normal Heart" on May 19, 2014. (Photo credit: Alberto E. Rodriguez/Getty Images North America)
Jenna Ushkowitz and her boyfriend Michael Trevino looked happy while posing together on the red carpet. (Photo credit: Alberto E. Rodriguez/Getty Images North America)
The couple couldn't keep their hands off each other while they attending an event together. (Photo credit: FayesVision/WENN)
Jenna Ushkowitz and her boyfriend Michael Trevino started dating in 2011 and first publicly debuted their relationship in the same year. (Photo credit: Frazer Harrison/Getty Images North…


text: Powertown is able to provide faster service because we are able to obtain quality replacement and OEM parts for almost any brand of outdoor power equipment. Most orders arrive in under two days except for an occasional part that requires ordering directly from the individual dealer. For units not easily transported, we provide service from a fully equipped van.


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Published: Saturday 21st February 2015 by The News Editor
Almost 100 firefighters and 15 fire engines are tackling a blaze which has broken out at industrial units.
One unit is completely alight while two others have been affected by the fire, which sparked more than 50 emergency calls to the fire brigade from concerned members of the public in south-east London.
While no homes have been affected London Fire Brigade has advised people living near the scene at Yarnton Way in Erith to close their doors and windows due to large volumes of smoke.
Station sanager Terry Gooding said: "We're working hard to bring the fire under control. The blaze is in an industrial area and is not affecting any residential homes.
"There is though a lot of smoke and I'd advise local residents to close doors and windows. We are likely to be at the scene throughout the morning dealing with the blaze."
The force said the cause of the fire, which broke out around 2am, is not yet known.
Published: Saturday 21st February 2015 by The News Editor


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This is one of my two "local" expresso stand. I actually happened to come across this one accidentally, as it's one my path from daycare to the freeway.
The barista always kno...
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text: Hi all... I just finished building my PC164 system with a Millennium G200 and Intel Ether Express Pro 100 and Tekram 390F card and it comes up into ARC just fine. I'm booting a custom-built 2.2.13 kernel with minimal support (SCSI, Ethernet, FP completion) and it runs fine for a few hours, but then suddenly zonks out. The monitor says "no signal" and the reset button doesn't do any good. The ethernet LEDs go out. Power cycling makes the keyboard LEDs do a happy dance, but there still is no video signal. FYI I took the green wire for power on (from the power supply) and grounded it against a ground wire. The only partial remedy is for me to unplug the motherboard from the power supply, take out the battery, and let it sit overnight. Then it'll come up okay, but only until it does it again. I was running a 2.0.36 kernel that came with the boot disks (debian) and that was running for about five days. Could this be software related? If it was software related, why wouldn't a power cycle fix it? thanks in advance for any help anyone can give me, -- Christopher Kyin-hwa Chen <firstname.lastname@example.com> <http://moocow.reed.edu/~cchen/> "I just wish they would let us use the fire trucks and get them all wet. (The protesters) would get cold and leave pretty fast." -- Washington State Police Riot Squad Trooper, Seattle, 2 Dec 1999


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Cast your votes for the best animal figures of last year (2014) in our grand final poll!
Nice! I def want a mantis shrimp! Maybe both, but def the mantis shrimp!
Love that mantis shrimp!
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… Hearts, & Roots, … Heart Shape, Drawing – Art Product, Love, Romance, Drawn, Drawing – Activity, Painted Image, No People, Representing, Blue, Cloud, Stars, Surrealism, Surreal, Art, ArtWork, My Art, Painting, Bleeding Heart , Grassland, Landscape, Acrylic Painting, Symbolism by Alexandra Cook on EyeEm
… well, when I started this Painting, yesterday I thought it will be a nice cute Valentine Card, Design, . Of course, it went quite far in a Surrealistic, direction since … Drawing – Art Product, Close-up, Artists Life, Art, ArtWork, Hearts, Heart, Tree, Cloud, Stars, Paintbrush, Acrylic Painting, My Artwork, Creativity, рисунок, Arte, Brush, Love, Bleeding Heart , Roots, Surrealism by Alexandra Cook on EyeEm
… a Pink, Textile, Jewelry, Heart, now in my Linandara Etsy shop … Plant, Close-up, Pink Color, Leaf, No People, Crafts, Textiles, Handmade, Wool, Felt, Aloe, Necklace, One Of A Kind , Hand Made Jewelry, Hand Made, Etsy, Arts And Crafts, Love, Valentine, For Sale, Pink Hearts by Alexandra Cook on EyeEm
A pendant made by me with my own design. Water and Fade- proof. Look for Linandara on Etsy ($24) Pendant, Jewelry, Jewellery, Handmade, Pebble, Love, Peace, Drawing, Heart, Handmade Jewellery, One Of A Kind , Кулон, сердце by Alexandra Cook on EyeEm
Unless we are very, very careful, we doom each other by holding onto images of one another based on preconceptions that are in turn based on indifference to what is other than ourselves. This indifference can be, in its extreme, a form of murder and seems to me a rather common phenomenon. We claim autonomy for ourselves and forget that in so doing we can fall into the tyranny of defining other people as we would like them to be. By focusing on what we choose to acknowledge in them, we impose an insidious control on them. I notice that I have to pay careful attention in order to listen to others with an openness that allows them to be as they are, or as they think themselves to be. The shutters of my mind habitually flip open and click shut, and these little snaps form into patterns I arrange for myself. The opposite of this inattention is love, is the honoring of others in a way that grants them the grace of their own autonomy and allows mutual discovery.
The first step to take is to become aware that love is an art, just as living is an art; if we want to learn how to love we must proceed in the same way we have to proceed if we want to learn any other art, say music, painting, carpentry, or the art of medicine or engineering.
Someone once asked me what I regarded as the three most important requirements for happiness. My answer was: 'A feeling that you have been honest with yourself and those around you; a feeling that you have done the best you could both in your personal life and in your work; and the ability to love others.'
There was a question in my feed recently: What to do if you are for freedom of expression but there is somebody with ISIS flag marching throght the town? I think people should get organized and ready for the occasions like this – to get out and surround this person with all sorts flags for peace, freedom, love and equality.
There was a question in my feed recently: What to do if you are for freedom of expression but there is somebody with ISIS flag marching throght the town? I think people should get organized and ready for the occasions like this – to get out and surround this person with all sorts flags for peace, freedom, love and equality.
Now the one undeniable fact: this disillusion is inevitable. You had to ideal. You married did ideal, then along comes a fact that does not correspond to that ideal. You suddenly notice things that do not quite fit with your projection. So what are you going to do when that happens? There's only one attitude that will solve the situation: compassion. This poor, poor fact that I married does not correspond to my ideal; it's only a human being. Well, I'm a human being, too. So I'll meet a human being for a change; I'll live with it and be nice to it, showing compassion for the fallibilities that I myself have certainly brought to life as a human being.
One of the boldest things you could possibly do would be to marry that ideal that you've fallen for. Then you face a real job, because everything has been projected onto him or her. This goes beyond lust; this is something that goes way down. It pulls everything out. This anima/animus is the fish line that has caught your whole unconscious, and everything's going to come up – the Midgard Serpent, everything down in the bottom. This is what you marry.
For one human being to love another; that is perhaps the most difficult of all our tasks, the ultimate, the last test and proof, the work for which all other work is but preparation. I hold this to be the highest task for a bond between two people: that each protects the solitude of the other. This is the miracle that happens every time to those who really love: the more they give, the more they possess.
Letters to a Young Poet


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Cornerback – Michigan
2017 Fantasy College Football Outlook
There was no college football outlook written for Jabrill Peppers in 2017.
January 10, 2017 Jabrill Peppers News
RotoWire Fantasy Analysis
Peppers declared for the 2017 NFL Draft on Tuesday, Nick Baumgardner of MLive.com reports.
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Recent RotoWire Articles Featuring Jabrill Peppers
The Spread Offensive: Answering the Burning Questions
Published November 15, 2016
Jesse Siegel answers the questions that are ripping through the college football landscape. Who will win the Big Ten?
More Jabrill Peppers Articles View Last 30
The Spread Offensive: Lesson Learned, Hopefully November 8, 2016
The Spread Offensive: All is Falling Into Place October 31, 2016
The Spread Offensive: Who Can Derail Alabama Train? October 18, 2016
The Spread Offensive: Top-10 Showdowns on Tap September 27, 2016
A blank stat line is used above whenever a player was not on the field for any plays in the game that week.
Jabrill Peppers: Past News Updates ( ▲ View most recent update )
December 30, 2016
RotoWire Fantasy Analysis
Peppers (hamstring) will not play in Friday's Orange Bowl against Florida State, per the ESPN broadcast.
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October 31, 2016
RotoWire Fantasy Analysis
Peppers carried the ball five times for 24 yards and one touchdown against Michigan State on Saturday.
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text:
Maya Angelou, one of America's most celebrated poets, novelists and civil rights activists has passed away. Angelou, who served on two presidential committees and was awarded numerous awards, was 86 years old.
Published at 9:55 AM EST on May 28, 2014 | Updated at 10:04 AM EST on May 28, 2014


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Book Review: Saltwater Vampires
I debated whether or not to write a review for this book. But after looking at the reviews on Goodreads and seeing that the rating is pretty average, I decided to add my two cents. Because this is a fantastic book and it has not gotten enough praise! I know we all might be tired more »


text: by Clare Plested on 12th August 2016 Clare Plested has been sampling cocktails around Edinburgh for Broadway Baby every day for a week now. If you see her, offer her the 12-step programme."Spreading my wings and trying an 80 Days Daiquiri at the IBIS Southbridge. Delish!!"Clare Plested: Flock UpVenue: PBH Free Fringe – Ciao Roma (Venue 283)Dates: 6th to 27th August 2015 (not 17th) Time: 5.50pm (6.50pm)


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The Brokerage Citylink and Tower Hamlets Education Business Partnership held a joint conference on Friday 27th January to explore what the world of work will look like by the year 2040.
Business leaders from across London discussed the future of careers within professional and financial services, with perspectives given from businesses and educationalists on how employers and educators have a role to play in preparing tomorrow's workforce.
The conference, 2040- what work will look like for the next generation, was hosted by Lloyd's, who are a key supporter and partner of both charities.
Matthew Ryder QC, Deputy Mayor for Social Integration, Social Mobility and Community Engagement, opened the conference.
Speaking at the event, the Deputy Mayor said: "London is an amazing City of opportunity and we need to make sure it stays that way. Today's children and young people will be London's future - key workers, business leaders and entrepreneurs - people whose skills and capabilities will be the future of this great City. We, who are working now, need to make sure that we are creating the environment that they can then build on".
In a rapidly changing market, where it is estimated that 65% of children entering primary school today are likely to work in roles that don't currently exist, leading corporates are already addressing changing labour market needs and how businesses and schools will need to adapt and prepare for life in the second half of the century.
Lord Willetts, Executive Chair of Resolution Foundation and former Minister for Universities and Science, delivered the keynote speech, highlighting that businesses continue to offer poor career routes to the 40% of young people who do not gain a graduate qualification.
Business perspectives were provided by Chris Beazley, CEO of London Market Group, who focused on the talent challenge for the London market, and Anthony Bruce, HR Partner at PwC, who spoke about trust, adaptability and leadership in 2040 workplaces.
The conference highlighted the need for specific collaboration between businesses and educationalists to give students an understanding of what the real working world looks like, enabling them to translate their knowledge and skills from the classroom to the boardroom.


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What does independence mean to you?
Independence means freedom what we are enjoying today due to the Great freedom fighters, the entire credit goes to them.
Your favorite freedom fighter?
Every freedom fighter had their own way of getting freedom and independence. Equal due respect to all of them. All the freedom fighters are important. We should thank them since it because of them that we see this day.
One of your favorite patriotic songs?
'Challe Challo'
Independence day quote for your fans?
Happy Independence Day to all the viewers of india-forums. Enjoy and celebrate this day of freedom and thank all the freedom fighters for their contribution and making us see this very day. Also thanks for supporting me, I have an 'Actor' word before my name because of you all. Thank you very much. Keep reading and writing to india-forums.com because I am a daily visitor of this website and read all your comments and wants from my end. Working hard to fulfill all your expectations.
For more such articles keep reading India-Forums.com
Reporter and Author: Krishma Solanki.


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Home Authors Posts by Mary Renzi
Mary Renzi
8 POSTS 0 COMMENTSIn addition to writing articles for the beloved Dirge Magazine, Mary Renzi writes fiction of the dark and speculative variety, which can be found in such publications as Hypnos, One Throne, Pantheon, Zymbol, decomP, and Deadly Chaps. She enjoys masochistic endurance events, wildland firefighting, and running long distances in the brutal desert heat of Phoenix, AZ.
The Complete Human Being: Occult Tactics for Body/Mind Mastery
Occultists have spent centuries trying to master the link between body and mind. Here we look at some of their processes.
Laird Barron's Swift to Chase is a Paranoid Joyride Through an Arctic Hell
Laird Barron's latest book is Swift to Chase, a collection of interconnected short stories, each taking place in Alaska.
Death and Illumination: The Search for the Demiurge Within Us All
Many societies -- secret and otherwise -- have sought illumination and even otherworldly power through the use of ritual initiation.
Transgressive Authors All Rebel Intellects Must Read
"Transgression" is a word with heavy connotations. In Catholic-speak, a transgression is a sin, a violation of God's law which requires punishment. In our modern-day litigious culture, transgression means roughly the same thing, only with punishment meted out by a social authority rather than...
Elemental Horror: The Dark Places of Lars von Trier
The films of Lars Von Trier are not lighthearted, campy fun. These films are dark, emotional journeys straight into the black heart of humanity.
The Lesser-Known Triumphs of Aleister Crowley
You probably know him as a cannibalistic Satanist, but Aleister Crowley was actually a mountain-climbing, chess-playing, yoga-practicing, normal human.
Hungry For Creation: The Relentless Art and Music of Jacob Bannon
You may know him as the vocalist of hardcore band Converge, but did you know Jacob Bannon is also a supremely talented artist?
If it Bleeds, it's Leeds: Matthew M. Bartlett's 'Gateways to Abomination'
If you like dark, strange, twisted, interconnected short stories, do yourself a favor and get Matthew M. Bartlett's "Gateways to Abomination."


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CINCINNATI, Ohio – The Cincinnati Cyclones, ECHL affiliate of the NHL's Florida Panthers and Nashville Predators, announced that goaltender Magnus Hellberg has been assigned to the club.
A second-round selection (38th overall) by the Predators in the 2011 NHL Entry Draft, Hellberg appeared in a pair of games last year for the Cyclones and went 1-1-0 with a 2.52 goals-against average. The 22-year-old native of Uppsala, Sweden has spent the bulk of his pro career in the AHL with the Milwaukee Admirals where over the past two years, he's compiled a 27-26-1 record along with a 2.38 goals-against average. He made his NHL debut with the Predators on Oct. 26, 2013 against the St. Louis Blues.


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We have investigated urine protein inhibitors of calcium oxalate crystallization to determine whether variations in these proteins are associated with kidney stone disease and whether protein measurements improve the identification of stone formers compared with conventional risk factors (RF). Using Western blotting, we studied variations in the electrophoretic mobility patterns and relative abundances of crystallization-inhibitory proteins in urine from 50 stone-forming (SF) and 50 non-stone-forming (NS) first-degree relatives of calcium SF patients, matched by gender and age. Standard urine chemistry stone risk measurements were also made. Multivariate discriminant analysis was used to test the association of these proteins with nephrolithiasis. Differences in form and abundance of several urine proteins including inter-α-trypsin inhibitor (ITI), prothrombin fragment 1 (PF1), CD59, and calgranulin B (calB) were found to be associated with stone formation. By multivariate discriminant analysis, measurements of forms of PF1, ITI, and calB in men and ITI and CD59 in women, classified 84% of men and 76% of women correctly by stone status. In contrast, standard urine chemistry RF identified only 70% of men correctly and failed to distinguish female SF from NS. Thus a small subset of protein measurements distinguished SF from NS far better than conventional RF in a population of relatives of calcium SF, illustrating the significant association of these proteins with stone disease. Variations in these proteins may serve as markers of stone disease activity or vulnerability to recurrence and may provide new insights into mechanisms of stone formation.
- kidney calculi
- crystallization
- inhibitor
- inter-α-trypsin inhibitor
- prothrombin fragment 1
- calgranulin
a number of proteins in urine have been implicated in the process of calcium kidney stone formation, due to either their ability to inhibit crystallization of calcium oxalate (CaOx) and calcium phosphate stone-forming (SF) salts or their presence in the matrix of these stones. Such proteins include Tamm-Horsfall protein (THP) (20), osteopontin (uropontin) (2), prothrombin fragment 1 (PF1) (38), inter-α-trypsin inhibitor (ITI) (9), and bikunin (BK), a portion of ITI (3), calgranulin (34), CD59 (7), and albumin (10). Although some reports suggest defects, deficiencies, or variations of these molecules among SF vs. normal subjects (13, 14, 26, 27, 43), it remains unclear whether any of these is a cause or a consequence of stones; no research thus far has translated molecular knowledge into clinical practice.
Among the many proteins that have been linked in some way to stone formation, it is possible that some are more strongly associated with nephrolithiasis than others. If this is the case, certain protein measurements may be better at distinguishing SF from none-stone-forming (NS) in a population, using statistical techniques of classification. We wish to identify those proteins that are most strongly associated with stone formation to focus attention on those that may offer the greatest insights into the disease.
Our approach here has been to measure multiple urine proteins in groups of SF and NS subjects, all of whom are first-degree relatives of calcium SF patients. We reasoned that by making our comparisons within SF families, we would reduce the effects of well-known inherited stone causes such as hypercalciuria so that possible links between protein biomarkers and being a SF or not might be more readily observed.
Study Subjects
During a concurrent study of families with calcium stone disease (National Institute of Diabetes and Digestive and Kidney Diseases Grant R44-DK-54585), 24-h urine samples were obtained from 902 first-degree relatives of calcium SF. From this pool, we chose 100 subjects at random, one-half female, one-half SF by sex to create a balanced block design of 4 cells (male NS and SF, female NS and SF) with 25 subjects/cell. The incidence of stones was self-reported; documentation of stone type was unavailable. We excluded subjects with dipstick proteinuria or glucosuria above trace, to avoid potential effects of renal disease. Also, we included only subjects who were not taking medications that could affect stones or mineral metabolism. These medications included thiazide and other diuretic agents, vitamin D supplements, and alkali supplements.
To select our set of 100, we used a random number table, choosing the first eligible SF in the list that met an arbitrary criterion, an even number. Thereupon, we selected as a NS control the first same-sex subject within 5 yr of age, meeting the second criterion, an odd number on the random table. We continued this process until we had obtained our set of 100, which is a true random sample of the original 902. These 100 subjects arose from 74 families, each of which contained a SF proband. No probands are included here. For 58 of the 74 probands, we had stone analyses or documentation of calcium stones according to their physicians' records. For the remaining 16 probands, we had knowledge that stones were not struvite, cystine, or uric acid, and they were therefore classified as "calcium" SF.
The ethnic backgrounds of the 100 subjects were Caucasian (96%), Asian (2%), and African-American (2%). Ages ranged from 23 to 69 yr. Our randomization was successful in relation to age, weight, and conventional urine stone risk factors (RF), including 24-h urine volume, urine pH, daily excretion of protein, calcium, citrate, magnesium, ammonia, sodium, sulfate, oxalate, phosphate, creatinine, and uric acid and calculated supersaturations for CaOx, calcium phosphate, and uric acid. The mean values of these measurements did not differ among the four groups of male and female SF and NS (Table 1). Urine inhibition of CaOx crystal growth was measured as an end point in the original study of relatives of SF (6, 19); these four groups did not differ in that measurement (Table 1).
Conventional Studies
Each participant was sent a kit containing materials to collect a 24-h urine specimen and return two 50-ml aliquots to our laboratory. Gentamicin (20 mg) and 1.25 g of Germall II (International Specialty Products, Wayne, NJ) were added to a 4-liter container as urine preservatives before collection was begun. One aliquot was used for the measurement of established stone RF, including calcium, oxalate, citrate, phosphate, magnesium, uric acid, sulfate, ammonium, chloride, potassium, sodium, and pH using methods already detailed from this laboratory (1). Urine creatinine was measured to assess the completeness of collection. Supersaturations for CaOx, calcium phosphate, and uric acid were calculated using EQUIL 2 (48).
Preparation of Dialyzed Urine Proteins
The second 50-ml aliquot was dialyzed using a 3,500-Da membrane (Spectra/Por, Spectrum Laboratories, Rancho Dominguez, CA) at 4°C for 40 h against 10 mM NaCl, 5 mM Tris, pH 7.2, with three changes of the bath. The protein concentration was measured using the BCA method (Pierce Biotechnology, Rockford, IL). This protein mix was used for the assay of crystal growth inhibition (6). An aliquot was also frozen at −20°C and subsequently used for gel electrophoresis and Western blotting.
Western Blotting
Knowing that the proteins of interest can exist in various forms in urine, we chose at the outset to examine these proteins by Western blotting to visualize what we were indeed measuring. At the time, no commercial source provided purified standards for these proteins, and therefore we were limited to a semiquantitative assay in which the optical density (OD) of the proteins was expressed as a fraction of the total OD on a track after staining with specific antibody followed by chemiluminescent Western detection.
Gel electrophoresis.
Proteins were resolved by PAGE using the method of Laemmli (23) with the Mini-Protean III electrophoresis system (Bio-Rad Laboratories, Hercules, CA). Precast gels (18% resolving with 4% stacking gels) were purchased from Bio-Rad. Gels were run under denaturing and reducing conditions. Chemiluminescent protein molecular weight standards (MagicMark) were from Invitrogen (Carlsbad, CA). Protein samples (20 μg) were solubilized by boiling for 3 min in the presence of SDS and 2-mercaptoethanol before loading on gels. Proteins were separated by SDS-PAGE at 90 V for 2 h.
Blotting and Western detection.
After electrophoresis, gels were immersed for 15 min in transfer buffer composed of 39 mM glycine, 48 mM Tris, 0.0375% SDS, and 10% methanol. Proteins were then transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad) using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad) at 30 V overnight. Following electrotransfer, the PVDF membrane was rinsed in TTBS buffer (150 mM sodium chloride, 25 mM Tris, pH 7.2, containing 0.05% Tween 20). Western detection was carried out using the ECL chemiluminescence kit (Amersham Biosciences, Piscataway, NJ). Nonspecific binding sites were blocked by immersing the membrane in 1× Blocker BSA (Pierce Biotechnology) in TTBS for 1 h at room temperature. The membrane was washed in TTBS and incubated for 1 h in a solution containing sheep polyclonal antibody to human prothrombin fragment 1 (CL20111AP, Accurate Chemical and Scientific, Westbury, NY) diluted 1:2,000 in TTBS. The membrane was again washed in TTBS, then incubated for 1 h with a donkey anti-sheep IgG secondary antibody conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA) at a dilution of 1:10,000. Chemiluminescence of the ECL substrate was detected by digital image acquisition using a cooled CCD camera system (BioChemi System, UVP, Upland, CA). Exposure time was 1 min. Antibodies were stripped from the membrane using Restore Western blot stripping buffer (Pierce Biotechnology) according to the manufacturer's instructions. A second Western detection was then performed as above by incubating the membrane with rabbit polyclonal antibody to human inter-α-trypsin inhibitor (A0301, Dako, Carpentaria, CA) diluted 1:2,000 followed by goat anti-rabbit IgG secondary antibody conjugated to horseradish peroxidase (Pierce Biotechnology) at a dilution of 1:10,000. Additional blots were prepared for analysis of CD59 or calgranulin B (calB) using 60 μg of urine protein. Detection was performed as above using goat polyclonal antibody to human CD59 (SC-7076) or calB (SC-8114) diluted 1:2,000 and donkey anti-goat-HRP secondary antibody at 1:10,000 (all from Santa Cruz Biotechnology) with an exposure time of 1 (calB) or 10 min (CD59). All secondary antibodies (as purchased) were preadsorbed with human serum proteins to minimize cross reactivity to other proteins in urine.
Primary antibodies used for the detection of THP, albumin, α-1-microglobulin, and calgranulin A (calA) were sheep anti-human THP (Cortex Biochem, San Leandro, CA), sheep anti-human albumin (Biodesign International, Saco, ME), sheep anti-human α-1-microglobulin (Biodesign International), and goat anti-human calA (Santa Cruz Biotechnology). Appropriate secondary antibodies and the same dilutions were used as above.
Data Analysis
Image analysis.
Digital images were stored in established quantitation software (LabWorks, UVP). By lane, each image was processed to measure location and intensity of all bands. Location was in molecular mass units taken from conventional standard lanes. The optical density (OD) of each band and the total OD of the lane were determined by the software, then intensities of each protein were calculated in three ways. First, the OD of each band was divided by the total OD of the lane to yield a unitless ratio that expresses the OD of each band as a percentage (p) of the total for that individual sample. The OD fractions of the bands added up to 100% for each person, but mean values for the individual groups of men and women, with and without stones do not add up to 100%, as expected, because means of ratios are not equal to ratios of means. Second, the OD of each band was divided by the total OD of a standard urine sample lane that was run on every gel. This semiquantitative corrected OD can be used to compare abundances of each band between samples and gels in absolute (a) terms. Finally, the total OD in the lane was divided by the total OD of the standard lane to express total (t) immunoreactivity for the protein as a fraction of that of the standard urine.
Statistical analysis.
Molecular mass and intensity data were ported to standard statistical software (Systat 11, Systat Software, Richmond, CA) and merged with panels of age, sex, SF status, crystal growth inhibition, and stone risk urine chemistry data. We chose age and sex as demographic demarcators because both are well known to have effects on mineral metabolism and prevalence of stone disease. The association of individual variables with stone formation was estimated using a combination of simple univariate t-tests and multivariate discriminant analysis. Our strategy was to maximize discovery of possible protein relationships to stone forming. To do this, we essentially screened all protein measurements for differences by sex or stones using t-tests. As a more stringent screen, we performed ANOVA by protein in which protein abundance was the dependent variable with sex and SF as factors. Where ANOVA showed sex or stone-sex interactions of significance (P < 0.05) we performed post hoc hypothesis testing by sex as appropriate. To preserve both analyses, we show significance values by t-test as superscripts and those confirmed by ANOVA as bold superscripts (see Table 2). Finally, we included all proteins we measured as candidates in multivariate discriminant analysis.
Electrophoretic Profiles of Urine Proteins
By visual inspection of Western blots, a PF1 band with a molecular mass of 31 kDa predominated among most subjects (Fig. 1A). Many samples also contained a lower molecular mass (LMM) form of ∼23 kDa. Less abundant higher molecular mass forms (HMM: >40 kDa) of PF1 were also visible in some samples. A few samples also contained smaller immunoreactive bands (<20 kDa) that are probably products of proteolytic degradation of PF1.
BK migrated as a prominent band at ∼37 kDa, in agreement with the 35- to 40-kDa size of native urinary BK (Fig. 1B) (17). Most samples contained a second band of ∼21 kDa. Weakly staining HMM bands (>50 kDa) were visible in most samples, and a small portion contained LMM bands (<20 kDa).
CD59 migrated principally as a band of ∼20 kDa, consistent with the molecular mass of 18–20 kDa observed by others using SDS-PAGE (Fig. 1C) (29). In addition, samples from several subjects contained LMM bands, which may be either products of deglycosylation (28) or proteolytic cleavage.
CalB Western blots (Fig. 1D) showed a major immunoreactive band at 14 kDa that is the monomeric form of this protein (31). In addition, forms of ∼30 and ≥40 kDa were also apparent. Samples from several subjects contained LMM bands (<12 kDa) that are likely to be proteolytic cleavage products of calB (not shown).
Other proteins.
Similar Western blots were prepared to analyze other proteins of interest, including THP, albumin, α-1-microglobulin, and calA. No significant differences were detected in the urinary abundances of these proteins between SF and NS, and none were informative in any of our analyses; thus the blots are not shown.
Univariate Analysis of Isoform Abundances
Differences between SF and NS.
For clarity, we repeat here that we use "p" to denote the proportion of immunoreactivity ascribed to a single isoform, "a" to denote the abundance of the isoform compared with a standard, and "t" to denote the total immunoreactivity of all isoforms compared with the standard. Male SF had a lower proportion of PF1 in the >40-kDa band, HMM PF1 (p), than male NS, whereas female SF had more PF1 (t) than female NS (Table 2). Female SF had more 37-kDa BK (p) and less 21-kDa BK (p) than female NS, whereas male SF had higher 21-kDa BK (a) than male NS. Female NS had less CD59 (a) than female SF. Overall, the main stone-related differences were 1) decreased HMM PF1 (p) and increased 21-kDa BK (a) in male SF vs. NS; and 2) increased CD59 (a), PF1 (t), and 37-kDa BK (p) in female SF vs. NS.
Sex differences.
Female NS had greater 23-kDa PF1 (p) than male NS and, correspondingly, reduced 31-kDa PF1 (p). As a group, women had less BK (a) and less ITI (t) than men. Female SF had less 21-kDa BK (a) than male SF. Female NS had more 21-kDa BK (p) and less 37-kDa BK (p) than male NS. In addition, female NS had less CD59 (a) than male NS. Among both SF and NS, women had significantly more of the monomeric form of calB (a) than corresponding men.
The use of multiple t-tests offers the virtue of sensitivity in this kind of discovery research but suffers from well-known tendencies to find significances from chance alone. We therefore performed ANOVA by protein with sex and stone forming as factors and have bolded superscripts indicating significance. Significant differences with stone forming were found for BK (p) and 21-kDa BK (p) in women, 21-kDa BK (a) in men, and HMM ITI (p) for stones and sex, which was not found with t-tests.
Multivariate Analysis of Proteins in Relation to SF
As expected from Table 1, conventional RF were not useful in distinguishing SF from NS by multivariate discriminant analysis. When all subjects were considered together, the results were insignificant, with 55% of subjects correctly classified by stone status and only urine pH entering the model (P = 0.14). In men alone, citrate excretion and supersaturation of CaOx (SS CaOx) entered the model, but the overall success rate of classification was rather low (70%), and the P value was marginally significant (P = 0.05). In women, analysis by forward stepping failed to produce any classification by RF, as no variables entered into the model. For these reasons, we analyzed the sexes independently, and proteins separately from the RF.
For protein analysis, we included all measurements of every protein (including PF1, ITI, CD59, calA, calB, THP, albumin, and α-1-microglobulin) in the initial model. From this set, HMM PF1, HMM ITI, BK, and HMM and low-molecular-mass (LMM) forms of calB (Fig. 2) classified 84% of male subjects correctly (P = 0.0008). The false-positive and false-negative results were both low, at 16%. In women, CD59, BK, and HMM ITI correctly identified 76% of subjects (P = 0.003), with false-positive and false-negative rates of 24%. Although the sets of proteins contributing to classification were not the same for both sexes, BK and HMM ITI forms were common discriminators. Interestingly, entry of BK into either model does not appear to improve the results (Fig. 2), but analyses performed without this variable failed to achieve more than marginally significant results, indicating a complex relationship with other discriminating proteins.
Our results point to HMM ITI as a potential urine protein correlate of stone formation within families of probands who were themselves calcium SF. The ITI family of proteins consists of three heavy chains (H1, H2, H3) of 75- to 85-kDa molecular mass, and one light chain, BK, with a molecular mass of 35–40 kDa (17, 39). BK can be linked covalently through its chondroitin-4-sulfate side chain to one or two heavy chains, forming the pre-α-inhibitor (PαI; H3+BK, 125 kDa) or ITI (H1+H2+BK, 180–220 kDa) (8, 11, 36), also known as ITI-trimer (26). The >50-kDa bands we detected here most likely correspond to heavy chain components, PαI or ITI. The 21-kDa BK we found corresponds to either the ∼25-kDa BK form identified by Suzuki et al. (43) as deglycosylated BK or a 20-kDa fragment of BK, also known variously as urinary trypsin inhibitor 2 (UTI-2) (15, 22) or uristatin (37). LMM bands (<20 kDa) are products of proteolytic degradation.
In a less striking way, BK measurements contributed to discrimination in all subjects as well. Men and women differed in the types of HMM ITI and BK measurements entering the classification, whether a proportion (p) of immunoreactivity in that protein form within a sample or an absolute (a) amount relative to a standard urine control. The reason for this sex difference is not clear, but in any case HMM ITI and BK measurements were important discriminators.
There are conflicting reports in the literature regarding the relationship between ITI and stone disease. Medetognon-Benissan et al. (27) reported that BK levels were lower in SF than in healthy controls. By contrast, Suzuki et al. (43) found higher urinary concentrations of BK in male SF compared with healthy male controls and that a greater proportion of SF than normal patients had an aberrant BK 25-kDa species, which is identified to be deglycosylated BK, a form with reduced effectiveness in inhibiting CaOx crystal growth. Marengo et al. (14, 26) found increased HMM forms (PαI and ITI), but not BK, in SF (14, 26), and these differences were constant over several months (26).
Prothrombin fragments were strong predictors of stone forming in men. The abundant 31-kDa protein we observed is consistent with urinary prothrombin fragment 1, formerly referred to as crystal matrix protein (40). The 23-kDa form corresponds to either a thrombin-derived cleavage product of PF1 (33) or deglycosylated PF1 (35). HMM forms (>40 kDa) are likely to be other PF1-containing activation peptides of prothrombin, primarily prothrombin fragment 1+2, with a molecular mass of 43 kDa (40). Immunohistochemical analysis of human kidneys has shown that PF1 is synthesized in the proximity of renal stones and is more abundant in the kidneys of SF than in healthy individuals (41). However, to date no differences have been found in the relative amounts of any prothrombin forms in the urine of CaOx SF vs. normal controls (14).
PF1 and BK are potent inhibitors of CaOx crystal growth (3, 4, 38), and can be produced in the kidney (30, 42). While the entire ITI molecule has been shown to inhibit CaOx crystallization (9), the heavy chain components do not have inhibitory activity, and BK is thought to be responsible for the ability of ITI to inhibit CaOx crystallization (21). Interestingly, we observed here that urine from women contains a lower proportion of intact 31-kDa PF1 and less BK and total ITI than that from men. Such differences may well be a reason that we found reduced whole urine crystal growth inhibition among women vs. men (6).
In women, but not men, the amount of CD59 was successful in discriminating SF from NS. Little is known about the relationship of CD59 to stone disease. CD59 is an 18- to 20-kDa glycoprotein inhibitor of the membrane attack complex of complement that is thought to play a role in protecting the kidney from complement-mediated injury (47). CD59 deficiency and increased urinary excretion of CD59 have been linked to renal tissue damage (18, 24, 45). CD59 is an acidic protein with a modest ability to inhibit CaOx crystallization (Coe F, unpublished observations), but its possible association with nephrolithiasis has not been otherwise investigated.
Calgranulin is a strong inhibitor of CaOx crystal growth and consists of two monomeric subunits, A and B (also known as MRP8 and MRP14, or S100A8 and S100A9), which are small (11 and 14 kDa, respectively) acidic proteins that can combine to form homo- and heterodimers (24–28 kDa), as well as tetramers and other higher-order multimers (35–48 kDa) (16, 31, 34, 44). In fact, it is the calA/calB heterodimer for which crystal growth inhibition has been documented (34). Although the heterodimer is thought to be the functional form of the protein, there is increasing evidence that the monomers and other conformational forms may exert individual functions (31). We observed protein bands with sizes corresponding to monomeric, dimeric, and multimeric forms on both our calA and calB blots. However, we found that only calB-immunoreactive forms, but not calA, contributed to the discrimination of SF from NS in men. The relevant calB bands were in the HMM (>40 kDa) region of the gel, which may represent multimeric forms of the protein. However, it is not clear whether such forms would be expected to be visible under the denaturing and reducing conditions used here, as the proteins are noncovalently linked, so the identity of these bands is uncertain (44). LMM calB (<12 kDa), which also entered the classification model, most likely represents proteolytic cleavage products of various calB forms.
In addition to inhibiting CaOx crystallization, ITI, PF1, CD59, and calgranulin are known to have roles in mediating inflammation or cell injury and repair, and their role in the pathophysiology of nephrolithiasis may not be limited to or even involved with crystal inhibition (46). Expression of these proteins may be provoked by renal tissue damage from stones or crystals. Alternatively, these proteins may promote stone formation by acting as nucleators or as a matrix for crystallization (49). In any case, stone disease may induce changes in expression or posttranslational modification of PF1, ITI, CD59, and calgranulin in the kidney, which could consequently affect the form or abundance of these proteins in urine. We may well be observing kidney cell responses to the presence of stones or crystals, or perhaps injuries from stone-passage events.
The source of these proteins in urine is not yet certain, as they could be filtered, in part, from blood. However, HMM ITI forms, which were found to be important predictors of stone formation, would not be efficiently filtered due to their size. This suggests that HMM ITI in urine is of renal rather than hepatic origin (17, 26). What does seem reasonable, given the present work, is that these questions concerning the source and purpose of urinary ITI, PF1, CD59, and calgranulin are significant issues for subsequent research.
Technically, this study is limited by the electrophoretic conditions used to separate the proteins and the semiquantitative nature of the measurements. We can be reasonably sure that the PF1, ITI, CD59, and calB bands we have observed represent the molecular species that we have discussed in this report. However, the conditions we used here were initially chosen for the analysis of LMM proteins such as calgranulin and were not ideal for resolution and quantitation of HMM forms (>50 kDa).
Proteolysis could produce a shift from HMM to LMM species and be more prominent in SF than NS. Bautista et al. (5) found aberrant LMM OPN variants more frequently in SF than normal controls due to increased serine proteases in their urine. We have not observed that urine PF1, ITI, CD59, or calgranulin is susceptible to rapid degradation in the absence of protease inhibitors (data not shown). In any case, protease degradation is not a likely explanation for what we observe with ITI, because we do not detect the appearance of new aberrant LMM forms but rather an increase in the abundance of 37-kDa BK and HMM ITI forms in the urine of SF.
This study was designed so as to reduce differences of standard urine RF between SF and NS. Because all subjects were first-degree relatives of calcium SF, there was no difference in urine calcium levels between SF and NS here, whereas we (32) and others (25) have found higher urine calcium levels among SF vs. normal people. We chose these conditions to reduce the effects of routine factors such as urine calcium to see the effects of urine proteins against a more neutral background.
In conclusion, this study supports the hypothesis that variations in the abundance and electrophoretic mobility of specific urine proteins are associated with stone formation. In these relatives of calcium SF, PF1, ITI, CD59, and calB isoforms are candidates for identifiers of SF. The meaning and origin of the sex differences in these proteins, and the differences between SF and NS are not clear. However, even so, PF1, ITI, CD59, and calB do seem either to affect stone disease, respond to it, or, at least, be somehow related to stones through some intervening mechanisms. These molecules have great potential, as markers and predictors of SF propensity, or as clues to the development of new drugs for prevention of stones or renal crystallization. Given our own recent proofs that CaOx SF deposit interstitial apatite crystals in their inner medullary tissues (12), understanding and exploiting the properties of crystal inhibitors may well be of even greater clinical importance. If future studies show that cosegregation is robust and reproducible, it will be difficult to accept the null hypothesis that such proteins have no role in stone disease, as a cause of stones, or as a consequence.
This work was supported by National Institutes of Health Grants R44-DK-54585, R43-DK-64499, PO1-DK-56788, and Grant 01-49101 from the Illinois Department of Commerce and Community Affairs.
Brian Coe and Fredric Coe have financial interests in Litholink Corporation and are on the board of directors.
We thank Georg Müller for help with statistical analyses. We acknowledge the technical assistance of Kimberly Crocker.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2006 the American Physiological Society


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2017 Rams Roster Preview: Cory Harkey, At Closing
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2017 Rams Roster Preview: Cory Harkey, At Closing
If youe a UDFA, your self superior be prepared in the direction of grind absent.Ordinarily, a 5-yr vocation for a UDFA would appear to be together with a attractive strong profession, thinking of no person drafted on your own. That doesn feel plenty of for recent Los Angeles Rams TE and chief Cory Harkey, whilst.Youl in all probability uncover him inside the centre of a huddle delivering pre-sport speeches hoping toward attain his staff members organized for fight. For a gentleman who doesn get hold of substantially likelihood, Harkey contains been a robust participant for the Rams. As an H-Back again Los Angeles Rams Jerseys, component-season Facebook, within just-line TE and exclusive groups participant, he can do a little bit of every little thing.Is that a man the Rams require yet again for his 6th year as a Ram?Roster BattleHarkey greatest instantaneous roster overcome arrives towards freshly drafted 6th-spherical starter Facebook Sam Rogers (roster preview) https://www.ramsuniformshop.com/81-John_Sullivan_Jersey, instant-spherical choose TE Gerald Everett, and past year 2 drafted TEs: Tyler Higbee and Temarrick Hemingway (roster preview). At the moment, optimum of all those men are strictly acquiring TEs and don present the exact adaptability as Harkey.If everyone, Sam Rogers will be his most important competitors.ExpectationsIf youe seeking at regular figures, no, Harkey almost certainly won include several quantities if any at all. Nonetheless if youe searching for a chief, filthy operate model participant who can provide your self lucrative snaps inside the jogging sport and upon ST, then Harkey will absolutely include some form of a part.Opportunity of Developing Ultimate Roster (6 Isaiah Johnson Jersey.5/10)If it have been not for Rogers, I consider this # would be large. Even then, this is a participant moving into his 6th yr with heaps of enjoying knowledge and a locker-area voice. Inside this Storystream 2017 Los Angeles Rams Roster Preview 2017 Rams Roster Preview: LB Mark Barron, Headhunter 2017 Rams Roster Preview: Cory Harkey, At Previous 2017 Rams Roster Preview: CB Kayvon Webster A Mainstay?
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I'm kind of in love with slow cooker smoked pork. It is so dang easy to make I end up making it all the time. I use it in all sorts of things including these killer quesadillas that we absolutely love. It actually got me thinking about a lettuce wrap version. It's healthier and all that. These wraps are delicious! Not only are they healthy with vegetables and all, but they are so easy to make and tasty at that. You can make your carrot veggie mixture at any point during the 8 hour slow cooker time. Just refrigerate until you are ready to use.
Prep Time: 15 minutes
Slow Cooker Time: 8 hours, low
Yield: Serves About 6
1 (2-3 lb) pork butt roast
3 TB liquid smoke
1 TB sea salt
2 carrots, shredded or julliened
1 cucumber, peeled, seeded, and julliened
3 TB rice vinegar
12 romaine lettuce leaves
2 cups fresh cilantro leaves
1/2 cup hoisin sauce
1. Place the pork roast into a 5 to 8 quart slow cooker. Pour liquid smoke over pork and sprinkle with sea salt. Cook on low 8 hours until meat shreds easily with a fork. Shred before serving.
2. Before serving, combine carrots and cucumbers in a mixing bowl. Pour rice vinegar over and toss to coat.
3. Serve shredded pork on lettuce leaves, topped with some of the carrot mixture, fresh cilantro leaves, and a drizzle of hoisin sauce.
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|RMA One on One Free Agribusiness Sessions for Farmers
|Date: Wednesday, February 22nd, 2017
|Time: 4:00 PM - 8:30 PM
|Location: TAC 24 Hyde Ave Vernon CT
For more Information Please CLICK HERE.
One-on-One Advising Sessions 2017
9am-3:30pm (most locations) except TAC – Tolland County Extension Center,4-8:30pm)
Wednesday February 22th,
Tolland County Extension Center, TAC - 24 Hyde Avenue, Vernon, CT 4 – 8:30 pm
Saturday March 4th
CT Farm Bureau Office, 78 Beaver Road Wethersfield, CT 06109
Monday March 13rd
Middlesex County Extension Center, 1066 Old Saybrook Road, Haddam CT
Saturday March 25th
Common Ground High School, 358 Springside Ave. New Haven, CT
Thursday March 30th
New London County Extension Center, 562 New London Turnpike Norwich, CT
Monday April 10th
Wamogo High School, Agri-Science Center 98 Wamogo Rd. Litchfield, CT
Tuesday April 18th
Fairfield County Extension Center 67 Stony Hill Rd. Bethel, CT