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|N�mero de publicaci�n
|Tipo de publicaci�n
|N�mero de solicitud
|Fecha de publicaci�n
|15 Nov 2011
|Fecha de presentaci�n
|4 Nov 2010
|Fecha de prioridad
|8 Ago 2003
|Tambi�n publicado como
|N�mero de publicaci�n
|Judith Kelleher-Andersson, Karl K. Johe
|Cesionario original
|Neuralstem, Inc.
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|Citas de patentes (49), Otras citas (233), Clasificaciones (24), Eventos legales (2)
|Enlaces externos: USPTO, Cesi�n de USPTO, Espacenet
This application is a divisional of U.S. Ser. No. 12/500,073 filed Jul. 9, 2009 and now allowed, which is a continuation of U.S. Ser. No. 12/049,922 filed Mar. 17, 2008, now U.S. Pat. No. 7,560,553, which is a divisional of U.S. Ser. No. 10/914,460 filed Aug. 9, 2004, now abandoned, which claims benefit under 35 U.S.C. �119(e) to U.S. Ser. No. 60/493,674 filed Aug. 8, 2003. The contents of these applications are incorporated herein by reference.
This invention relates to the use of fused imidazoles, aminopyrimidines, nicotinamides, aminomethylphenoxypiperidines and aryloxypiperidines for use as therapeutic agents and analytical reagents. More particularly this invention relates to these agents as therapeutics for prevention and treatment of neurological conditions and diseases in mammals and reagents for detecting neurogenesis and proliferation.
According to a long-held doctrine, no significant numbers of neurons are made in the adult mammal to contribute to the function of the adult mammalian nervous system. However, recent data indicate that adult mammalian brains contain neural precursor cells capable of generating new neurons both in normal and in injured conditions. These new neurons have been quantified in live animals by injecting or feeding in drinking water a marker of dividing cells, bromodeoxyuridine (BrdU) and by immunostaining of post-mortem brains with antibodies against BrdU and neuronal markers. An endogenous marker of dividing cells, Ki-67 protein, has also been used instead of BrdU for this purpose. Thus, in healthy, young rodents, approximately 3,000-15,000 new cells per day are estimated to be born in the dentate gyrus of the hippocampus, about 60% of which express early neuron-specific proteins such as doublecortin and type III beta-tubulin. Significant numbers of new cells and new neurons have also been observed in healthy, young primates. In rodents as well as in primates, the location of neurogenic areas in the central nervous system (CNS) is limited to the dentate gyrus of the hippocampus and the subependymal layer of the striatum. In human patients of different ages who have been diagnosed with a tumor of the tongue, a single injection of BrdU has revealed significant number of new cells and new neurons being born in the dentate gyrus and the subependymal layer of the striatum. Thus, adult mammalian brains contain neural stem cells capable of differentiating into neurons, and this process of generating new neurons (neurogenesis) occurs in the mature, adult brain in significant quantities throughout rodents, primates, and human species.
Such significant quantities of new neurons suggest that the new neurons may be important for the normal physiology of the brain, especially the hippocampus. The hippocampus is the main area of neurogenesis in adult rodents and is central for key cognitive functions such as learning and memory where new information is added, edited, stored, and recalled constantly throughout life.
Since the hippocampus is the most potent neurogenic area of the brain, many studies have been undertaken to establish whether neurogenesis may be the cellular mechanism to structurally accommodate the ever-increasing volume of cognitive processing to be handled. Thus, it has been shown that at least some of the newly born neurons, marked by genetic markers, mature to be electrophysiologically active and integrate into the exisiting neuronal circuitry of the hippocampus. Ablation of the neurogenesis in rats leads to decreased cognitive capabilities in several behavior tests. Thus, the existing data demonstrate that neurogenesis significantly contributes to the normal hippocampal physiology.
For example, most antidepressants are thought to work by increasing the levels of monoamines available for post-synaptic receptors. Examples of classes of agents working apparently by the "monoaminergic hypothesis of depression" include the selective serotonin uptake inhibitors (SSRIs) like fluoxetine, the mixed noradrenaline/serotonin transporter blockers like tricyclic agent imipramine and noradrenaline uptake inhibitors like desipramine. The antidepressant-induced increase in intraneuronal biogenic amines occurs quite rapidly. However, the antidepressant-induced improvement in clinical behavior requires weeks of daily administration.
One hypothesis that may account for the slow-onset of the antidepressants' therapeutic activity is that they work by promoting hippocampal neurogenesis. It is expected that neurogenesis would require a number of weeks for stem cells to divide, differentiate, migrate and establish connections with post-synaptic neurons. The neurogenesis theory of depression then postulates that antidepressant effect is brought about by structural changes in the hippocampal circuitry contributed by newly generated neurons stimulated by antidepressants (Malberg et al., 2000; Czeh et al. 2001; Santarelli et al, 2003).
The neurogenic theory of depression, though not conclusive, has strong supportive data including the finding that neurogenesis is actually requisite for antidepressant behavioral improvement in the novelty suppressed feeding model (Santarelli et al., 2003). A therapeutic benefit from hippocampal neurogenesis is further supported by the finding of hippocampal atrophy in depression, where magnetic resonance imaging studies identified a reduction in the right and the left hippocampal volumes in individuals with major depression (Sheline et al., 1996; Bremner et al., 2000; Mervaala et al., 2000). Long-standing work also suggests a strong relationship between glucocorticoid dysregulation or glucocorticoid hypersecretion in stress and depression, such that the hippocampal volume loss might be considered a consequence of glucocorticoid-induced hippocampal neuronal loss (Sheline et al., 1996; Lucassen et al., 2001; Lee et al., 2002 (review)). Furthermore, in studies which involved the administration of a chronic stress to animals, both hippocampal volume changes and reduction in neurogenesis are observed, and theses events are both reversed by chronic antidepressant administration (Czeh et al., 2001; Pham et al., 2003), further illustrating the strong association between depression, stress and neurogenesis. The association comes full circle, since agents or conditions that promote a reduction in neurogenesis also appear as causative agents/events in depression, specifically glucocorticoid (Sapolsky, 2000), and depletion of serotonin (Brezun and Daszuta, 1999), Kempermann and Kronenberg (2003), though acknowledging the validity of the hippocampal neurogenesis theory of depression, suggest that this hypothesis needs to be looked at in the context of a larger model of cellular plasticity, which elucidates how antidepressants induce nascent neurons of unknown phenotype to survive and produce viable circuits.
Neurogenesis can be characterized as three successive stages: proliferation of endogenous stem cells and precursors, differentiation into neurons and neuron maturation with formation of viable synaptic connections (plasticity). In consideration of each of these stages of neurogenesis, the hippocampal volume loss in depression could potentially be caused by 1) inhibition of the endogenous hippocampal stem cell proliferation in the dentate gyrus, 2) inhibition of differentiation and dendrite development and 3) loss of neurons (apoptosis) and their dendritic structure. Though apoptosis, also known as programmed cell death, is observed in depression, hippocampal apoptosis, as measured by DNA fragmentation, from depressed patients appears to play only a minor role in the volume loss (Lucassen et al., 2001).
In an animal model of acute stress or in normal animals receiving exogenous corticosterone, the stress causes a reduction in synaptic plasticity in the hippocampus (Xu et al., 1998). Chronic administration of the tricyclic antidepressant, imipramine, partially reversed the loss in long-term potentiation (LTP) in socially stressed, depressive-like animals (Von Frijtag et al., 2001) suggesting imipramine affects the plasticity phase of neurogenesis.
In another animal model of depression characterized by loss of neurogenesis and hippocampal volume loss, stressed animals that chronically receive the antidepressant, tianeptine, show similar numbers of dividing cells as control animals (no social stress) a measure of proliferation (Czeh et al., 2001).
In an experiment looking at association of antidepressants and neurogenesis in normal adult rats, the antidepressant, fluoxetine, required chronic administration to cause proliferation of cells in dentate gyrus (24 hrs post-treatment), but there was considerable loss of nascent cells, whether in the presence or absence of fluoxetine treatment, where fluoxetine provided no observed differentiation or survival benefit (Malberg et al., 2000).
Results on different neurogenic intervention points by known antidepressants suggest that novel neurogenic agents that intervene at different points in the neurogenesis pathway could result in potentially diverse therapeutic effects on depression. These points of intervention can be studied and the target elucidated for novel antidepressant candidates through in vitro assays. Since adult stem cell proliferation and neurogenesis is observed in adult vertebrates in hippocampal dentate gyrus (Gould et al., 2001; Eriksson et al., 1998), we can use multi-potential hippocampal stem cells to screen agents in vitro for neurogenic activity.
Interestingly, chronic administration of either the antidepressant fluoxetine, an SSRI or the antidepressant rolipram, a phosphodiesterase IV inhibitor, promoted neurogenesis in normal animals (Malberg et al., 2000; Nakagawa et al., 2002). One might conclude from these results that any agent that promotes neurogenesis will generate a behavioral benefit in depression, unrelated to the agents mechanism-of-action or possibly that there is a common pathway where both drug actions overlap. D'Sa and Duman suggest a scheme whereby the phosphorylation and activitation of CREB and the subsequent expression of BDNF are central to the induction of neurogenesis that culminates in antidepressant behavior. CREB phosphorylation is increased in animals administered rolipram chronically (Nakagawa et al., 2002), and antidepressants that either increase Ca2+/CaM-kinases of cAMP could cause the phosphorylation of CREB in the nucleus (reviewed by D'sa and Duman 2002). D'Sa and Duman (2002) further suggest that the phosphorylated CREB then binds to the CRE binding site to promote the expression of BDNF and bcl-2, that appear critical to cell survival and plasticity. Proof of involvement of neurotrophic factor BDNF in depression comes from studies showing that antidepressants and electroconvulsive shock both caused an increase in BDNF levels (Nibuya et al., 1996) and that intrahippocampal injection of BDNF had antidepressant activity in two models of depression (Shirayama et al., 2002).
If neurogenesis is critical for antidepressant activity is it also sufficient for therapeutic activity and is the mechanism by which the neurogenesis occurs or timing of neurogenesis also critical to the therapeutic activity? These questions can be answered by using novel agents developed through screening paradigms that identify agents that promote the proliferation and differentiation of endogenous hippocampal stem cells to neurons in vivo and determine fi they will be effective antidepressants.
In abnormal conditions, such as when an injury to a brain area has occurred, neurogenesis becomes more wide-spread and perhaps functionally diverse. In rodent models of ischemic and hemorrhagic stroke, the newly born neurons of the subependyma (also referred to as subenticular zone) are seen migrating to and accumulating in the lesion area of the cortex. However, the newly born neurons have a short survival period.
Neuropathology associated with key cognitive regions of the Alzheimer's diseased brain has led to therapeutic strategies that address the neuronal loss, in the hopes of reducing the cognitive decline. One strategy enlists trophic agents, that regulate neuronal function and survival, as Alzheimer's Disease (AD) therapeutics (see Peterson and Gage, 1999). Problems with systemic administration, side effects and locating trophic-sensitive neurons generated few clinical successes with these therapies. One AD therapeutic, AIT-082, promotes memory enhancement in AD individuals potentially by stimulating endogenous trophic factors (Ritzman and Glasky, 1999; Rathbone et al., 1999). So the use of agents to promote increased survival and function of the remaining available neurons appears to have some therapeutic value.
As discussed, the hippocampus is one of the main brain regions where neurogenesis in adult brain has been documented across several vertebrate species, including monkeys and humans (e.g., Gould et al., 2001; Eriksson et al., 1998). In fact, adult hippocampal neurogenesis contributes functionally to cognitive capacity. Shors et al. (2001) reported that inhibition of neurogenesis in adult rat hippocampus, in the absence of the destruction of existing neurons, caused impaired memory function. Many studies observed that degenerative conditions induced neurogenesis in mature mammalian brains, suggesting the existence of a natural repair pathway by means of neurogenesis. A focal ischemic model, reversible photothrombic ring stroke, caused increased neurogenesis in rat cortex by 3-6% (Gu et al., 2000). Seizures induced by electroconvulsive shock in adult rats increased neurogenesis in dentate gyrus of hippocampus *Scott et al., 2000; Madsen et al., 2000). Also, rats gamma-irradiated to kill mitotic cells in the CNS showed reduced numbers of nascent neurons and reduced LTP in slice cultures. These observations highlight the likelihood that a cellular mechanism for neurogenesis within adult human CNS, especially in hippocampus, does exist both as a normal physiological process and as a self-repairing pathway.
In adult neurogenesis a decline due to aging is observed (Kuhn et al., 1996), though proof that this age-dependent decline in neurogenesis causes cognitive impairment is still debated. Considerable evidence does exist, indication that increased neurogenesis reduces age-associated cognitive decline. This is most dramatically observed with the transplantation of human stem cells into aged rats initiating improved water maze learning and retention (Qu et al., 2001). Other data suggests that induction of neurogenesis by diet restriction (lee et al., 2000) exercise (van Praag et al., 1999) or growth factor addition (Lichtenwalner et al., 2001) improves learning and memory in adult or aged rats. A number of other inducers of neurogenesis have been identified, including anti-depressants (Malberg et al., 2000; Czeh et al., 2001), and nitric oxide donors (Zhang et al., 2001) suggesting the usefulness of neurogenic agents for other diseases presenting cognitive-deficits, such as stroke and depression. A small molecule that induces hippocampal neurogenesis that is blood brain barrier penetrable would allow for a potentially novel oral therapeutic for Alzheimer's disease.
Other potential AD therapeutics progressing in clinical trails, target neurodegeneration in the hopes of reducing the neuronal loss and cognitive decline. Apoptotic death involving caspase pathways and DNA fragmentation has been measured in in vitro and animal models of AD and in Alzheimer's diseased brain tissue. The extent of apoptosis leading to neuronal loss is of continual debate with most agreeing it has some effect, but that other neuronal death pathways definitely play a role (see Behl, 2000; Broe et al., 2001; Roth, 2001). Concern that measures of upstream caspase markers in neurons from AD tissue may not proceed to degeneration has been suggested (Raina et al., 2001). In order to screen for a neuroprotectant therapeutics it is critical, therefore, to measure more than one endpoint of neuronal death and determine at what point an agent may intervene in the death pathway(s). Behl (2000) suggested that AD pathology is most likely a mixture of apoptotic and necrotic pathways and that concentrating therapeutic discovery using only one pathway may provide inconclusive results. All hits in our neurogenesis models were tested through our secondary apoptosis/necrosis assay to screen for agents that function both as neurogenic and neuroprotective agents. These agents may improve or reverse the cognitive decline observed in MCI and AD.
Therefore, recent studies indicate that neurogenesis occurs in the adult human brains under normal as well as under degenerative conditions and that such adult-generated neurons do contribute functionally to the brain physiology such as learning and memory. These observations highlight the likelihood that a cellular mechanism for neurogenesis within adult humans CNS, especially in hippocampus, does exist both as a normal physiological pathway and as a self-repairing pathway. What is not known is whether deficiencies in the volume or persistence of neurogenesis and/or the survival or maturation of the new neurons contribute to permanent damage.
Thus, a compound that can stimulate endogenous neurogenesis, either in a disease state or in a healthy state, may be an effective drug for a number of human nervous system conditions and diseases. Many neurological diseases, including Alzheimer's disease, mild cognitive impairment, dementia, age-related cognitive decline, stroke, traumic brain injury, spinal cord injury, and the like, are neurodegenerative conditions. Neuropsychiatric diseases including depression, anxiety, schizophrenia and the like also show nerve cell dysfunction leading to cognitive, behavioral, and mood disorders. A neurogenic drug or agent that enhances the process of generating new neurons (neurogenesis) would be beneficial for countering and treating these diseases.
Candidate drugs generated from the screening have been tested in various animal models of human neurological and psychiatric disorders to determine the drugs' therapeutic potentials. An effective, predictive in vitro assay that can be used to select for clinical drug development neurogenic compounds that is particularly effective in promoting the neurogenesis in vivo has been described in U.S. patent application Ser. No. 10/728,652 filed Dec. 5, 2003, which is incorporated herein by reference in its entirety.
The present invention relates to compounds that promote neurogenesis in vivo. More particularly, the present invention is related to classes of compound structures that are shown to be particularly effective in promoting neurogenesis including compounds of the type, fused imidazoles, aminopyrimidines, nicotinamides, aminomethyl phenoxypiperidines and aryloxypiperidines. These compounds are shown to promote neurogenesis by proliferation and/or differentiation of human hippocampal multipotent stem cells and/or progenitor cells and neuronal progenitors. Moreover, the present invention relates to these agents as therapeutics for prevention and treatment of neurological diseases in mammals and as reagents for detecting neurogenesis and proliferation.
In one embodiment of the present invention, compounds are evaluated for their ability to promote neurogenesis by proliferation/differentiation of human hippocampal multipotential stem cells and neuronal progenitors and whether small molecule agents of the above chemical structures that have neurogenic activity in vitro and/or in vivo also have the ability to inhibit neuronal death. Modulation of neurogenesis pathways may overlap pathways critical to apoptotic and necrotic neurodegeneration, therefore, neurogenic small molecule agents are tested for their ability to inhibit neurodegeneration.
The compounds of the present invention are shown to stimulate endogenous neural stem cells capable of differentiating into neurons in adult human brains to proliferate and to differentiate into functional neurons in vivo. The additional neurons may enhance the cognitive ability of the subject and significantly extend the ability to perform cognitive tasks during extended periods of sleep deprivation.
The present invention also includes the identification of a specific class of compounds, the aminopyrimidines, that appear especially effective at inhibiting apoptosis in various neurodegenerative models suggesting this class of compounds might be especially useful for neurodegenerative indications.
The present invention includes neurogenic drugs which serve to prevent or treat neurodegenerative and neuropsychiatic disorders by promoting the endogenous birth of new neurons within the nervous system by administering the compounds of the present invention to the patient alone or in combination with transplanted stem cells or progenitor cells.
In a further embodiment of the present invention, the compounds are used as reagents for detecting neurogenesis and proliferation in an in vitro assay.
In one embodiment of the present invention, an agent is administered to treat a neurodegenerative disease. In a preferred embodiment of this invention the neurodegenerative disease includes Alzheimer's disease, dementia, mild cognitive impairment, aged-related cognitive decline, Parkinson's disease, amyotropic lateral sclerosis, multiple sclerosis, demyelination, stroke, spinal injuries, traumatic brain injuries, neuropathic pain, and the like.
One embodiment of the present invention includes an agent administered to treat a psychiatric disease. In one embodiment, the psychiatric disease includes depression, post-traumatic stress syndrome, acute or chronic stress, anxiety, schizophrenia, sleep deprivation, cognitive dysfunction, amnesia, and the like.
One embodiment of the present invention includes an agent administered by any number of routes including combining with multipotent stem cells or differentiated multipotent stem cells transplanted into brain.
In yet another embodiment of the present invention, the agent is administered to treat a cognitive dysfunction, memory deficit, or decreased learning capacity.
In a further embodiment of the present invention, cognitive dysfunction includes sleep deprivation, moderate cognitive impairment (MCI), and the like.
In another embodiment of the present invention, the agent is administered to enhance cognitive function, memory, and/or learning capacity of an individual.
In another embodiment of the present invention, an agent is administered by at least one route and at least one multipotent stem cell or differentiated multipotent stem cell is transplanted into brain.
In another aspect of the present invention, the agent is utilized in the above methods. Additional features and advantages of the present invention are described in, and will be apparent from, the following Detailed Description of the Invention and the figures.
Additional features and advantages are described herein, and will be apparent from the following Detailed Description and the figures.
The present invention relates to the identification of a specific type of compounds including fused imidazoles, aminopyrimidines, nicotinamides, aminomethyl phenoxypiperidines and aryloxypiperidines for use as therapeutic agents and analytical reagents by means of promoting neurogenesis by proliferation/differentiation of human hippocampal multipotent stem/progenitor cells and neuronal progenitors. More particularly this invention relates to these agents as therapeutics for prevention and treatment of neurological diseases in mammals and reagents for detecting neurogenesis and proliferation.
The key activity of a neurogenic agent is to increase the number of neurons generated from their precursors. A neurogenic agent can bring about such an increase in the number of neurons by a number of different mechanisms. The neurogenic agent can increase the number of neurons generated from their precursors (neurogenesis) and/or can protect existing neurons from neurodegeneration (neuroprotection).
The neurogenic agent can increase neurogenesis by acting as a mitogen for the neural stem/progenitor cells and, thereby, increasing the progenitor's cell number which, in turn, results in increased number of neurons in the culture when differentiated. Another mechanism includes the neurogenic agent acting as a neuronal specification factor by promoting the stem/progenitor cell differentiation forward neurons at the expense of glia. This directed differentiation will also result in increased number of neurons in the culture, but without changing the overall cell number. In yet another mechanism, the neurogenic agent can act as a mitogen for committed neuronal progenitors that differentiate only into neurons. Increasing this subpopulation would also increase the final number of neurons in the culture. Alternatively the neurogenic agent can act as a neuroprotectant to decrease neurodegeneration by acting as a survival factor to rescue immature neurons from undergoing cell death during differentiation, which will result in increased neurons.
Initial compound libraries include directed-libraries based on mechanistic pathways or targets thought to be involved in mitosis, differentiation, and survival of neural cells. These targets include growth factor receptors (e.g., FGFR, EGFR, NGFR), signal transduction pathways such as ras, CREBP, protein kinases and phosphatases, cell-cycle regulators such as c-myc, p53, p21, transcription factors such as bHLH proteins and nuclear hormone receptors, extracellular membrane (ECM) proteins such as metalloproteinases, and stress-related factors. These targets represent a collection of non-overlapping pharmacophores which cover diverse chemical space and, at the same time, leas to rapid identification of structure-activity relationships.
A neurogenesis screen of components to discover a safe drug which can activate the stem cells within the brain of a subject, especially in the hippocampus, and which can recruit new neurons into the circuitry to broaden the neural functionality at the physiological level must be capable of identifying a compound that will significantly boost either of these processes. In vitro assays in 96-well format have been designed to detect the effect of a compound on the proliferation and differentiation of hippocampal stem cells. These assays have been used to screen compound libraries for activities that stimulate mitosis and/or differentiation of hippocampal stem cells and/or progenitors. Candidate drugs generated from the screening will be tested in various animal models of human neurological and psychiatric disorders to determine the drugs' therapeutic potentials.
The compound identified as potential neurogenic agents include compounds with the following general structures:
Culturing a Stable Cell Line of Neural Progenitors
A screening of a large number of unknown agents (e.g., protein factors, peptides, nucleic acids, natural compounds, of synthetic compounds) for discovering a candidate drug involves repeating the same test for hundreds to millions of times. This repeated testing requires high level of reproducibility from the test. In order to obtain such reproducibility for a neurogenesis assay, stable cell lines of neural progenitors are required which, upon differentiation, generate reproducible quantities of neurons. For this purpose, a cell line is defined as a population of cells having been expanded for at least ten cell-doublings.
Human and other mammalian neural stem cells have been isolated, expanded, and differentiated, in culture from all major areas of the brain and spinal cord. (see for example, U.S. Pat. Nos. 5,753,506 and 6,040,180). Hundreds of stable, characterized, and cryopreserved neural stem cell lines have been isolated from many areas of the human and rodent brains including the hippocampus. In one embodiment, a multipotent neural stem cell/progenitor cell line derived from human hippocampus is used. As discussed above, the hippocampus is known for its relatively high level of neurogenesis. Cell lines derived from other areas of the central nervous system (CNS), including the dentate gyrus of an adult brain, can also substitute for the hippocampus. A neural progenitor population derived as a stable cell line from partial differentiation of embryonic stem cells can also be used.
Although cell lines that are not genetically engineered are preferred, in one embodiment, cell lines that are genetically engineered to enhance the mitotic capacity of the cells are used to test potential neurogenic agents. In one embodiment, the genetic modification consists of intracellular over-expression of functional c-myc protein under a conditional activation system such as a c-myc protein fused to a ligand-binding domain of an estrogen receptor as described in U.S. patent application Ser. No. 09/398,897 and filed Sep. 20, 1999, and incorporated herein by reference.
In one embodiment, a progenitor population that, upon differentiation, generates both neurons and glia in a single culture has been used. Presence of glia, either astrocytes and/or oligodendrocytes, or their precursors, are required to promote physiological maturation of nascent neurons born from their precursors in culture.
In one embodiment, differentiation of the progenitors is initiated by withdrawing the mitogen from the culture. It is preferable that serum as well as other growth-promoting factors, are removed from, or not added to, the differentiating culture due to their significant effect on the reproducibility of the neurogenesis assay.
Detection of Cell Proliferation
Neural stem cells and progenitor cells differentiate spontaneously in the absence of a mitogen, therefore, undifferentiated mitotic cells are harvested by enzyme treatment to remove residual mitogen, such as basic fibroblast growth factor (bFGF) used in expansion and proliferation of the cells. In one embodiment of the present invention, undifferentiated human hippocampal stem cells (e.g., HH580)/progenitor cells are harvested by enzyme treatment.
The collected cells are seeded for attachment of the cells such as standard 96-well or 384-well plates, pre-coated with extracellular matrix proteins, such as poly-D-lysine and fibronectin (e.g., Biocoat PDL, Fisher). The initial seeding density can be within the range of about 2,000-125,000 cells per well of a 96-well plate. The preferred density, however, is 40,000 cells per well of a 96-well plate which has been optimized for best signal-to-noise ration as described in pending U.S. patent application Ser. No. 10/728,652. Cell density that is too low retards the initiation of differentiation and results in poor plating efficiency which interferes with the assay. Cell density that is too high leads to inhibition of neurogenesis due to cell-to-cell interaction and paracrine factors which are also interfere with the assay. It should be appreciated that the actual cell number can be proportionally decreased or increased depending upon the surface area of the culture substrate used. For example, for a 384-well plate which has approximately one-fourth of the surface area of a 96-well plate, the initial seeding density should be decreased by one-fourth accordingly.
The cells are passaged and grown for seven days in 15 cm plates in full growth medium according to Neuralstem protocol with 50% of the media changed every other day. Following centrifugation, the cell pellet is re-suspended in N2b medium and enough N2b medium is added to the cell suspension to achieve a concentration of 4�105 cells per ml. The N2b seeding media includes a standard serum-free, growth factor-free, basal media without phenol red that supports healthy neuronal/glial survival. Fibronection and PBS is completely removed by aspiration from wells of fibronectin-coated 96-well plate(s). 100 μl of N2b medium with or without 2� concentration of screening agents or compounds to be tested are added to each well. Subsequently, 100 μl of the cell suspension is added to each well at a density of 40,000 cells per well. The cell suspension is incubated for two days at 37� C. in the presence of 5% CO2.
On Days 2, 4,, and 6 of post-plating, incremential additions of the compounds are made to each well at appropriate concentrations. Therefore, after two days, 100 μl of N2b medium is removed from the wells, and 100 μl of the N2b medium is added. Likewise, on days four and six, 100 μl of N2b medium is removed from the wells and 100 μl of the N2b medium is added, and the cell suspension is again incubated at 37� C. in the presence of 5% CO2.
On day seven, the final day of the culture, a fluorescent dye such as Alamar Blue dye is added to each well. Alamar Blue dye detects metabolic respiration and is a reflection of total cellular activity indicating changes in cell number of neurons. In this regard, 20 μl of Alamar Blue (Biosource International DAL 1025) is added to each well and incubated for two hours at 37� C. in the presence of 5% CO2.
As a preliminary detection of positive activities, the overall immunostaining intensity in each well is read by a fluorescence plate reader. For instance, the fluorescence of the oxidized dye in each well is read by a fluorescent plate reader such as the Molecular Devices Fluorometric Plate Reader. The reader is set on the Read Mode End Point setting at an excitation of 530 nm, an emission of 590 nm, and a cutoff 570 nm. The fluorescence level is proportional to the number of respiring cells in the culture and is a measure of a proliferative activity of a test agent. The data is then exported and saved into any suitable database such as a Microsoft Excel Workbook.
After the Alamar Blue assay to detect general cellular proliferation, further staining with other markers are required to determine what cell types (e.g. neuronal, glial) are observed under differentiation media.
Detection of Neurogenesis
The final neuron number is detected by immunostaining of the culture with antibodies against neurons and is quantified by counting of the immunopositive neurons and/or by measuring the staining intensity. Therefore, after seven days of compound treatment, the cells are stained with the neuron-specific anti-tubulin IIIb antibody, TuJI, for neurons and Hoechst for all cell nuclei. The number of TuJI+neurons per area is quantified and expressed as a value relative to the vehicle-treated control of Hoechst-stained cells. In this regard, the medium is removed from the wells be decanting and blotting the plates on paper towels. The cells are then fixed in 100 μl of 4% paraformaldehyde per well for thiryt minutes at room temperature. The cells are washed three times with 400 μl per well of phosphate-buffered saline (PBS) at pH 7.5 for approximately ten minutes per wash. The plates are then stored at 4� C. in PBS or used immediately for staining.
The cells are subsequently stained with antibodies against neuron-specific antigens according to standard procedures. The staining process includes decanting the PBS and blocking the cells with 100 μl of 5% Normal Goat Serum (NGS) in PBS for one hour at room temperature. The NGS/PBS is decanted and the plates are blotted on a paper towel. The cells are then permeabilized with 0.1% Triton X-100 in PBS for 30 minutes at room temperature.
Typical neuron-specific antigens include Type III-beta tubulin and MAP-2c. The total cell number in each well was quantified by staining the cultures with a nuclear dye such as DAPI of Hoechst according to standard procedures. Therefore, once the cells are permeabilized, 20 μl of TuJI, monoclonal antibody against neuronal class III β-tubulin (CoVance (BabCo) MMS-435P) and 32 μl of anti-GFAP polyclonal (DAKO Z0334) are added to and mixed of PBS with 5% NGS. Final dilutions of TuJI and anti-GFAP are 1:400 and 1:250, respectively, 90 μl of the antibody solution is added to each well except two eleventh column. 90 μl of PBS/5% NGS is added to the eleventh column and no cells are added to the twelfth column. The plate(s) are sealed with parafilm and incubated at 4� C. overnight.
After incubating the cells overnight, the antibody solution is decanted and 200 μl of PBS/0.1% Triton X-100 is added to the wells. The plate(s) s are placed on a shaker and incubated at room temperature with gentle shaking for ten minutes. The cells are again washed with the PBS/Triton X-100 solution another two times.
Following staining with TuJI and anti-GFAP, 20 μl of Alexa Fluor 488 labeled goat anti-mouse IgG (H+L) (Molecular Probes A-11001) and 100 ul of LRSC-IgG, goat anti-rabbit (Jackson Immuno 111-295-144) is added and mixed with 10 ml of PBS/5% NGS. 100 μl of the resulting antibody solution is added to each well. The plate(s) are covered with foil and incubated with gentle shaking for up to one hour at room temperature. The cells are again washed as described above with PBS and an Triton X-100. 200 μl of PBS is then added to each well and microscopy is performed to detect positive TuJI and GFAP staining.
If the cells are not positive for TuJI and GFAP staining, one 20 ul aliquot of DAPI (Molecular Probes) or Hoechst (Sigma Chemical) is diluted in 2 ml of PBS. 20 ul of this solution is then added to each well. The plates are left at room temperature for 15 minutes and then washed twice with PBS. After a final wash 200 μl of PBS is added to each well.
The plate(s) are then read by the Molecular Devices Fluorometric Plate Render to determine the ration of TuJI+cell number (neurons) to Hoechst-stained cells (total cells). The reader is set on the Read Mode Well Scan setting. For detection of TuJI/Alexafluor 488 staining the settings include an excitation wavelength of 495 nm, emission wavelength of 519 nm with auto cutoff, and a PMT Sensitivity set on high. For detection of GFAP/LRSC staining, settings include an excitation wavelength of 570 nm, an emission wavelength of 590 nm with auto cutoff, and a PMT Sensitivity set on medium. For detection of DAPI/Hoechst staining the excitation is set at 358 nm, the emission is set at 461 nm with auto cutoff, and the PMT Sensitivity is set on high.
Primary Screening of Unknown Compounds
Synthetic organic compounds for screening through the neurogenesis screen are pre-selected by predicted bioavailability and CNS permeability. Calculations for CNS permeability is based on the use of 300 descriptors for 1,474 known CNS therapeutics. Predicted properties for successful CNS drugs are determined to be: (1) molecular weight (MW) of 400 or less, (2) five or fewer hydrogen bond acceptors, (3) two or fewer hydrogen bond donors, (4) polar area below 120 angstrom, and (5) neutral or basic with a pKa between 7,5 and 10.5.
Since the screening of 10,000 small molecule compounds in in vitro models of neurogenesis has been completed the in vitro screen have been shown to be predictive of in vivo neurogenic efficacy, orally available agents can be tested, that promote in vivo neurogenesis in models of depression.
Rolipram, for example, an antidepressant that works by increasing cAMP levels and is neurogenic in animals (Nakagawa et al., 2002) was effective in a primary in vitro neurogenesis screen. This suggests that the primary in vitro screen includes those agents that might promote neurogenesis by targeting the cAMP/pCREB/BDNF pathway. This does not necessarily exclude all other neurogenesis mechanisms for the neurogenesic agents. If the target of these neurogenic agents is important for behavioral activity where three separate chemically diverse classes showed in vitro assay efficacy differences and that the mechanism for all does not overlap at the point of CREB phosphorylation of BDNF expression then one might expect very different effects on behavioral activities in depression models.
Cumulatively, over five thousand synthetic compounds of the type including fused imidazoles, aminopyrimidines, nicotinamids, aminomethyl phenoxypiperidines and aryloxypiperidines have been evaluated for their effect on neurogenesis according to the assay method described above. From the preliminary analysis using the fluorescent plate reader, over three hundred compounds show initial positive activity. Those three hundred compounds have been re-analyzed by quantitative neuron counting. Among them, thirty compounds significantly increase cell number or proliferation, fifty-three compounds increase the number of neurons neurogenesis; and seven demonstrated significantly activity in both proliferation and neurogenesis. The significance level was empirically set at an activity above 30% change over the vehicle control for proliferation and above 10% change for neurogenesis.
Sixteen compounds possessing exceptional in vitro neurogenic capacity have been identified and are listed along with their structures in Table I. The sixteen compounds have been tested for neurogenic activities in live mice as described below and in in vitro assays. Table II details the efficacy results of the sixteen effective small molecule agents. Based on these findings, the compounds have potentially effective therapeutic value for any number of neurodegenerative and neuropsychiatric disorders. These disorders include, but are not limited to, stroke, Alzheimer's disease, Parkinson's disease, depression, mild cognitive impairment (MCI), traumatic brain injury and the like.
Dose-Response Profiles
In another in vitro assay, compounds are tested as above at varying concentrations to obtain a dose-response curve and EC50 values (effective concentration of the compound which produces 50% of the maximum possible response for that compound). Compounds with EC50 values below 10 μM for neurogenesis or proliferation effect are considered further.
Primary dose-response profiles and secondary functional assays are used to further eliminate compounds from the primary screen based on a lack of dose-dependent effect on neurogenesis, indicated by lack of linearity of dose-response, and in vitro neurotoxicity. The dose-response curve measures neurogenesis over a concentration range of 10 pM to 100 μM. Examples of several primary hits fully analyzed for dose-response are shown in
Detection of Neuroprotection
Agents that promote proliferation and neurogenesis are further evaluated for their ability to inhibit neurodegeneration and apoptosis of the human hippocampal multipotent stem cell-derived neurons. Neurodegeneration may be induced in culture using any number of inducers as known by those skilled in the art. Examples of neurodegenerative inducers include: staurosporine, hypoxial reperfusion, free radicals, glutamate agonists, activated monocyte supernatant, beta amyloid peptides and corticosteroids to name a few. Some of the neurogenic agents inhibit loss of neurons, inhibit capase activation, inhibit nuclear fragmentation or condensation, inhibit lactate dehydrogenase activity among other effects on neurodegeneration. Using staurosporine as an example, 125 nM of a staurosporine solution is added along with 10 μM of one of the listed neurogenic agents to human hippocampal stem cells differentiated for 3-4 weeks.
A number of neurogenic agents inhibit neuronal loss, capase-3 activity and/or nuclear fragmentation compared to staurosporine vehicle control. These NSI small molecule agents are all effective at in vitro neurogenesis as determined by methods described. More particularly, human hippocampal stem cells differentiated for a period of 3-4 weeks are treated with an agent for 8-24 hrs in a neuronal maintenance media prior to treatment with an apoptotic or neurodegenerative insults such as staurosporin, TNFα and LPS-stimulated monocyte supernatant and the like. Following the treatment period, media is removed from the treated cells and the cells are fixed and stained. Straining may include Hoechst stain, MAP-2ab (AP-20) antibody staining, active caspase-3 antibody or other measures of neurodegeneration and apoptosis. Instrumentation such as a Molecular Devices fluorescent plate reader or a Cellomics Array Scall II instrument and the like may be used to measure the degeneration and apoptosis.
As illustrated in
Method for Measuring Caspase-b 3 Activity as a Measure of Apoptosis
Another assay used to determine the ability of a compound to provide neuroprotection is an apoptosis assay. Apoptosis is measured using the activity of an early marker of the programmed cell death pathway, caspase-3. Following 24 hours or 48 hours of apoptotic-induction in the presence and absence of compound treatment, the media is removed from the wells, and 50 μl cell lysis buffer is added to each well. 50 μl of 2� reaction buffer containing 10 mM DTT is then added to the lysate in each well. The contents of two wells are combined (for example: rows A/H, B/G, C/F, D/E), and 5 μl of substrates is added per well as recommended in the protocol for a caspase-3 fluorometric assay kit (Biovision) known in the art. The plate is incubated at 37� C. for 60 minutes. The contents of each well are then transferred to a fresh plate, and the fluorescence is determined with an emission wavelength of 400 nm and an excitation of 505 nm using a fluorescent plate reader such as the Molecular Devices Gemini Fluorescent Plate Reader. Caspase-3 activity is expressed as μM of cleaved substrate per hour. Substrate concentration in μM is run for each plate to determine activity values for each treatment condition.
In Vivo Neurogenic Effects of NSI Compounds
In one embodiment, fifteen of the compounds from Table 1 above are administered orally to mice at 10mg/kg for ten days and co-administered bromodeoxyuridine (BrdU) by intraperitoneal injection for the first six days. One class of compounds caused a significant increase in neurogenesis. This class, broadly defined as "isonicotinamides" and, more specifically of the structures, NSI-189 and NSI-158, were especially effective at promoting neurogenesis in the dentate gyrus of C57B16 adult mice following ten-day consecutive oral administration. For example, in
The ability to promote in vivo neurogenesis using these orally administered compounds suggest that these agents could be beneficial for a number of disease states including depression, aging, stroke, Alzheimer's disease and mild cognitive impairment, to name a few. With the production of new neurons in the granular cell layer these same compounds could promote cognitive enhancement to counteract loss of neurons due to disease, injury or age. These could be useful when cognition enhancement in humans might be beneficial for military and other purposes. It should be appreciated that other potential uses are contemplated herein and that this list includes only some of the potential uses for which these neurogenic agents appear ideally suited.
Further Studies of NSI-Compound Effect on the Stage of In Vitro Neurogenesis:
In another in vitro assay, compounds of the fused imidazole, aminopyrimidine, nicotinamide, aminomethyl phenoxypiperidine and aryloxypiperdine types are evaluated for their effect on the differentiation of the culture-born cells to mature neurons. More particularly, multipotent human hippocampal stem cells are treated in three doses over a one-week period in N2b media and in the presence of an agent of the type mentioned above. The cells are then fed twice weekly for approximately two more weeks in N2b or Neuralbasal+B27 Media (Gibco) and then cells are fixed using 4% paraformaldehyde. Cells are washed 3� with PBS (pH 7.4) and then stained using a nuclear stain (Hoechst or DAPI) and a mature neuronal marker, MAP-2ab (AP-20) antibody. A secondary antibody, Alexa Fluro 488 labeled goat anti-mouse IgG, is used to identify by fluorescent labeling the mature neurons. The fluorescent intensity and number of cells labeled in a multi-plate format can be measured using for instance a Array Scan II (Cellomics) or similar instrumentation.
Effects of the long-term NSI compound treatment on human hippocampal stem cells in the absence of mitogen is illustrated in
As illustrated in
As described in pending U.S. patent application Ser. No. 10/728,652, leukemia inhibitory factor (LIF), a cytokine growth factor, was selected from among several known neurotrophic factors as a positive control against which tested compounds can be compared. The selection of LIF as a positive control was based on its properties to increase the number of neurons and glia by two to three-fold. This effect is both consistent with the neural stem cell system in which the cells respond appropriately to the positive control by enhanced differentiation and/or mitosis, and achieves the objective of the assay method in which such cellular responses can be measured reproducibly and quantifiably. This, the use of LIF in
Results from NSI-106 and the positive control, LIF treatment are shown as an example. Unlike with LIF, NSI-106 produces mostly co-stained cells, indicating that the mitotic population is committed neuronal progenitors. The bars represent the mean�SD from each of six wells.
In addition, referring back to
The compound NSI-106 and similar agents could be useful especially in a more lipophilic form, as understood by those skilled in the art to promote division of neuronal progenitors in brain. This agent could be useful for a number of neurological indications. This agent and those like it, even in its present form, could be useful with any endogenous or transplanted stem cell to promote a neurogenic phenotype. The neurogenic phenotype could then be used for purposes of reversing or improving any number of neurological indications. For example, this agent could be given in utero or early postnatally for the purpose of improving brain or spinal nerve development. This compound might also be useful in stimulating embryonic stem cells to produce a neuronal progenitor that could be differentiated into neurons. Other uses of such a compound include hippocampal replenishment of neurons replacing, replenishing or enhancing any neuronal phenotype in brain, spinal cord and peripheral nervous system. This compound could then be useful for not only CNS indications but any nerve cell disease or injury.
It should be understood that various changes and modifications to the presently preferred embodiments described herein will be apparent to those skilled in the art. Such changes and modifications can be made without departing from the spirit and scope of the present subject matter and without diminishing its intended advantages. It is therefore intended that such changes and modifications be covered by the appended claims.
% Inhib
Neuron Number
2� Assay
% Prolif
(% of
(% of
% NeuronRatio
Nuclear Frag
211 � 48
77 � 12
92 � 6
0.3 nM, r20.59
0.07 nM, r20.75
16 � 19
92 � 3
165% @100 nM
157% @300 nM
137 � 8
120 � 5
181 � 14
81 � 13
104 � 8
0.1 nM, r20.65
1.5 nM, r20.5
103 � 8
114% @10 nM
137%@30000 nM
135 � 21
131 � 15
92 � 33
124 � 8
128 � 4
0.1 nM, r20.39
2164 nM, r20.70
115 � 11
144%@30,000 nM
202%@10000 nM
56 � 17
128 � 30
92 � 12
115 � 13
152 � 22
125 � 15
0.07 nM, r2065
0.001 nM, r20.70
105 � 26
123%@30,000 nM
175%@30000 nM
146 � 34
120 � 21
97 � 16
143 � 6
130 � 14
445 nM, r20.82
4.8 nM, r2 0.62
153 � 33
112%@30000 nM
115%@30000 nM
123 � 77
102 � 14
103 � 12
149 � 15
179 � 29
137 � 8
18.1 nM, r20.73
13.5 nM, r20.96
97 � 21
138%@300 nM
186%@300 nM
170 � 46
123 � 12
216 � 28
113 � 16
100 � 6
333 nM, r20.75
<0.1 nM, r20.66
47 � 15
89 � 13
111%@1000 nM
124%@1000 nM
171 � 19
143 � 11
177 � 44
107 � 29
99 � 6
<.01 nM, r20.77
85 nM, r20.90
110 � 25
112%@30000 nM
227%@3000 nM
120 � 10
105 � 7
218 � 140
135 � 15
142 � 6
>1000 nM, r20.67
>1000 nM, r20.75
146 � 19
146%@30000 nM
113%@10000 nM
139 � 43
Tox hi dose
Tox hi dose
107 � 22
236 � 79
93 � 17
106 � 9
36 nM, r20.48
0.2 nM, r20.88
22 � 18
99 � 6
106%@3000 nM
145%@300 nM
141 � 24
48 � 14
114 � 14
74 � 8
95 � 9
125 � 10
0.01 nM, r20.54
0.1 nM, r20.83
95 � 6
112%@10000 nM
123%@300 nM
85 � 45
78 � 34/
121 � 13
108 � 21
100 � 27
111 � 13
69 � 18
128 � 13
131 � 4
No Curve
0.05 nM, r20.60
99 � 19
112%@1 nM
140%@300 nM
40 � 33
148 � 30
111 � 16
73 � 26
141 � 70
135 � 15
>1000 nM, r20.895
786 nM, r20.538
30 � 19/13 � 12
83 � 25
112%@30000 nM
132% 3000 nM
155 � 30
105 � 19
70 � 21
102 � 41
130 � 19
411 nM, r20.82
253 nM, r20.76
144 � 17
141%@30000 nM
165%@30000 nM
121 � 74/182 � 39
110 � 26
96 � 19
76 � 14
130 � 22
127 � 9
<.001 nM, r20.36
13 nM, r20.64
42 � 25/32 � 1
98 � 11
128%@1 nM
165%@300 nM
121 � 19
100 � 12
108 � 28
151 � 40
116 � 8
>1000, r20.77
>1000 nM, r20.75
111 � 10
126%@30000 nM
145%@30000 nM
87 � 41
139 � 35
98 � 18
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In Defense of a Married Man Trailer (1990)
14 October 1990 Drama, Crime, TV Movie 94 mins
A lawyer's husband is having an affair. When the woman he is having the affair with is found dead. He is the prime suspect. Though he adamantly proclaims his innocence. His wife takes charge of his defense, but can she place his betrayal behind or aside to be able provide him with the best possible defense?
Watch the official In Defense of a Married Man 1990 trailer in HD below or find more In Defense of a Married Man videos on Vidimovie.

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Tagged: footer
February 22, 2014 at 2:54 pm #227906
I need little help with 2 things.
1. How to decrease footer height to fit text height (see picture) ?
2. How to display price excl. VAT in next line and with smaller font size (see pic.) ? I want do this on catalog page and on single product page. This is my setting of Price display suffix: incl. VAT {price_excluding_tax} excl. VAT
Thank you.
this is my page: acraft.sk
February 23, 2014 at 7:58 pm #228172
- This topic was modified 3 years, 9 months ago by miso00.
Hey miso00!
Please add following code to Quick CSS in Enfold theme options under Styling tab
#footer, #socket .container { padding: 0; } #footer .widget { margin: 0; }
#top .price span { float: left; clear: left; }
YigitFebruary 23, 2014 at 8:56 pm #228201
First code edit only footer but socket height is the same.
Second code works but incl.VAT and excl.VAT text is wrongly placed.
Is it possible to place prices in two lines through woocommerce "Price display suffix:" option ?
Site URL: http://www.acraft.sk
Thanks for your help.February 24, 2014 at 3:53 pm #228504
1- You do not have enough content on your page that is why socket section looks that high. Please add more content to your page
2- Can you post the link to your page where this happens
YigitFebruary 24, 2014 at 5:12 pm #228559
1. Yes, if I add more content, footer and socket looks good. But on some pages I don't want more content.
Is it possible to decrease socket height by some other way than adding content ? Thank you.
2. http://acraft.sk/en/product-category/diely-vzduchovych-podvozkov/audi/
And one other problem. Do you know how to remove category image from THIS page (audi logo above products)??
I want to have the category iamge only HERE. Thanks a lot.
February 25, 2014 at 10:06 am #228925
- This reply was modified 3 years, 8 months ago by miso00.
1) You can try to add a "Color Section" to the layout. Then use the "Section Minimum Height" option: http://www.clipular.com/c/6248100729454592.png?k=uv9y0eTazC1ex3LFe6HcMsFLH14 to set a minimum height for the content section. This height will be applied regardless of the content height. If the page is an archive page (i.e. shop page or post) you can't set a minimum height for the content. In this case you either need to add more content or the footer/socket area will be bigger.
2) I recommend to hide the image with some css code – insert this code into the quick css field:
.template-shop .page-thumb{ display: none; }
PeterFebruary 25, 2014 at 10:35 am #228933
Thanks Dude, Thanks Yigit. This helps me. You are the bosses.
Last thing. Please help me with price and incl.excl.VAT displaying. I mentioned it in (Purchase code hidden if logged out) /#post-228201″>THIS post (second image).
February 26, 2014 at 3:44 pm #229591
- This reply was modified 3 years, 8 months ago by miso00.
Please try adding following code to Quick CSS as well
#top .price { display: table!important; }
February 26, 2014 at 5:32 pm #229649February 26, 2014 at 6:06 pm #229670
- This reply was modified 3 years, 8 months ago by Yigit.
I have updated the code i have posted in my previous post, can you try it now
YigitFebruary 26, 2014 at 7:04 pm #229702
No effect. I have this in quickCSS:
#top .price span { float: left; clear: left; } #top .price { display: table!important; }
But if I add only this:
#top .price span { display: table!important; }
Prices looks like THIS. Stupid incl.excl.VAT text isn't next to price.February 26, 2014 at 7:06 pm #229706February 26, 2014 at 7:21 pm #229713
OK waitFebruary 26, 2014 at 7:25 pm #229718This reply has been marked as private.February 26, 2014 at 7:28 pm #229721
"Due to protection of web servers from repeated attacks, we were forced to restrict access to administrative interface of web pages to selected countries." I am from Lithuania
YigitFebruary 26, 2014 at 7:31 pm #229723
Can I change something in settings in order to acces the admin inteface by you ?
EDIT: Just changes some setting on my webhosting. Try now.
February 27, 2014 at 1:01 am #229846
- This reply was modified 3 years, 8 months ago by miso00.
You must be logged in to reply to this topic.


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- Please select a date to see the proper prices!
Description of the accommodation:
Rooms, services Should you be looking for a hotel in Japan, we suggest you take a look at the offers of Hotel Palace Inn Kagoshima. The hotel is offering 50 nicely furnished rooms to stay in to those seeking accommodation in Kagoshima. You are also welcome at the hotel for a fine local meal in the restaurant. The café provides a nice atmosphere for a romantic evening talk or a personal meeting. You can browse the Internet via wireless connection (WiFi) available at the hotel. If you wish to learn more about the exact scope of services provided by Hotel Palace Inn Kagoshima (including those available for an extra fee) or about the equipment of the rooms, you can find the complete list on the information sheet below.
Payment In order to finalize the reservation you may be required to provide credit card guarantee or advance payment by credit card, depending on the chosen room, date and price.
Arrival, opening hours The earliest time of check-in is 15:00, the latest time of check-out is 11:00. The personnel can speak Japanese and English languages.
Hotel Palace Inn Kagoshima rooms Rooms
- Please select a date to see the proper prices!
Accommodation services
Free WiFi in shared places
Own restaurant
Bathrobe, Hairdryer, Fridge, Tea/coffee-maker


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Hi Everyone!
We're proud to announce the another addition to the Suluta Family - Coveroo.com!
The "Coveroo Creed":
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Niall Horan enjoys LA gig with rumoured girlfriend Jessica Serfaty
Niall Horan enjoyed an evening out with his rumoured girlfriend Jessica Serfaty on Monday.
The Mullingar native and former America's Next Top Model contestant headed to The Troubadour to see friend Gavin James perform.
The pair appeared to keep a low profile and headed straight from the gig into Horan's car.
Horan (23) has been linked to the actress since March this year when they reportedly spent a weekend together in Las Vegas, although they have kept any relationship under wraps since.
The singer has also been linked to Selena Gomez and Celine Vandycke but hasn't confirmed any speculations.
Just 24 hours prior to their date, the former One Direction star performed his debut solo single, This Town, at the AMAs, and attended the awards alone.
Serfaty (25) was a contestant in America's Next Top Model in 2010, coming in firth place. She also starred in crime drama Blood Brothers in 2013.
The young model gave birth to a son, Roman, when she was 16. She married husband Ididia Serfaty aged 17, but the pair divorced in 2013.
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Fawry's spirit of success radiates from its most important asset: the Team . If you are enthusiastic and driven, be a part of our team of young and innovative professionals, please send us your resume and a little more about yourself and what you hope to accomplish.
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We know all of the intimate, romantic locations in Western Australia. Tell us your style and we will provide you with the inspiring ideas.
I've been photographing for about 20 years now and have enjoyed every moment of it!
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Faculties and Campuses
Turku University of Applied Sciences has four faculties. We train, for instance, Bachelors of Business Administration, Bachelors of Engineering, Bachelors of Culture and Arts and Bachelors of Health Care. We operate in Turku and Salo.
Faculties and Corporate Services
- Business, ICT and Chemical Engineering
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Corporate Services
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What's The Secret Of Turning A Single Story Into An Endless Franchise?
We know how (and where) franchises go to die, but what turns a one-off movie or a standard TV series into a franchise that can go on, sometimes even for generations?
6 Prequels That Surpass Their Originals
Most prequels are bad. This is due to many staying to close to the originals that they become pointless as we already know what's going to happen like The Thing prequel. Others are simply bad and result in massive inconsistencies and headaches such as with Star Wars Episodes 1-3.
There Is an Inherent Problem With Prequels
News of a sequel of a movie that you love can feel a little bittersweet: On one hand you get to revisit a fictional world that you love. On the other, there's a not insignificant chance that visit will be unpleasant and awkward. Even worse, though, can be news of a prequel.
Guardians of the Galaxy-style trailer makes the Star Wars prequels good
I'm really digging all these Guardians of the Galaxy-style trailers. This movie's vibe is so good and fun that it can even make the Star Wars prequels look good! What is this black magic? Maybe Disney should give all the footage to these editors and re-release the trilogy as something that makes some sense.
Holy bantha poodoo, Disney is making more Star Wars prequels
In what might be the most bombad idea of the year, a Disney executive has revealed that those Star Wars stand-alone movies that have been discussed will be prequels — specifically, origin stories, potentially focused on characters like Han Solo, Yoda, or that guy on Cloud City with the ice cream maker.
Movie and TV Trends We Hope Are On Their Way Out
Hollywood sells us quick hits of entertainment, and counts on our attention-deficit disorder to keep us distractible. But Hollywood also has its own case of ADD, chasing fads and cool ideas, and then running them into the ground. That can be a very good thing, because some fads are so dumb you can't wait for Hollywood…
New The Thing Trailer: Sheer Freaking Terror, Served Ice Cold
The first full-length trailer for the Thing prequel is here, and it serves up a huge dose of claustrophobia and paranoia, laced with awesome bloody violence. If you've been skeptical about a prequel to John Carpenter's film, check this out.
Bad news for Caprica: Syfy making another BSG prequel
Are there any gaps in the life story of William Adama you're dying to fill? Because Syfy has got your back. In addition to Caprica, they've just greenlit another prequel, focusing on the future Admiral's time as a viper pilot.
6 upcoming movie prequels, and why they might not suck
We caught our first glimpse of The Thing's creepy Arctic horror this past weekend. But it's just one of a half-dozen movie prequels in the pipeline. Past experience shows many of these prequels may suck. Here's your prequel suckitude forecast.
Ridley Scott Says Alien Prequel Will Have A New Female Hero — And Maybe New Giger Designs!
Ridley Scott says his new prequel to his seminal 1979 space-horror film Alien is definitely happening, and he's going to talk to venerable designer H.R. Giger about some new creature designs. And he drops some plot details. Spoilers ahead...
Arriving Pre-Spoiled: Why Prequels Suck
BSG spinoff Caprica launches next Friday, but as good as its opening episodes may be (And they are), we're still worried. Why? Because it's a prequel, and if science fiction has taught us one thing, it's that prequels generally suck.
10 Science Fiction Prequels, Ranked By Crappiness
It's prequel time, with Caprica newly out on DVD and both Star Trek and Wolverine hitting theaters soon. So we decided to rank 10 science fiction prequels in order of crappiness.
Prequels Aren't Just Dumb, They're Evil
Star Trek and Star Wars keep venturing down the dreary route of prequels, and in the process, they've made the universe a way less interesting place. But prequels aren't just boring and predictable — they're also morally wrong and a scourge on humanity, because they portray people as helpless pawns of a history that's…
Must See: Enterprise
Must-see TV shows are futuristic classics that shouldn't be missed. Of course, not every must-see is perfect. That's why we've rated them 1-5 on the patented "crunchy goodness" scale.
Title: Enterprise (later Star Trek: Enterprise)
Date: 2001-2005
Vitals: The early seat-of-your-jumpsuit days of Starfleet, complete…


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Rishita Manhattan – ...
₹ 4,000,000.00
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Aarcity Foreste, is One of the hottest sector in Noida Extension, Greater Noida, today as skyline of the country is changing. in this Humongous property Developments in Commercial, Residential and retail Sectors in Noida. In this Project Developed are testimony to the fact Indian real estate is growing at Lightning speed From Shopping malls to Multiplexes to integrated townships to office buildings-everything is being developed to match global standards. The Corridors Integrates the finest in living spaces with leisure Amenities and essential facilities like Indoor Games, Private Swimming Pool, Yoga Center, jogging Track, CCTV Camera for Security Purpose, 24 hrs Power backup and Water Supply or more. Your Dream Home Available with affordable Starting Price 22.75 Lac, and Possession Date is Dec 2016.
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Plaza Studio does Festivals about once a month, some website items go with us. After events we update solds. If today is an event day and you purchased an item that sold out you will be refunded, or put on a back order, whatever you prefer. We will contact you asap if this happens.
Click buttons to go to Plaza Studios social media sites
These imported Tribal dance belts have authentic coins and beaded discs.
This page is for the purchase of Letter G in the picture below (first on the left)
Belts shown above G, D, A, C Click here for other Tribal Belts


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Advice From Recruiters
EaglePicher Technologies (EPT) believes in placing individuals in positions that will benefit the growth and prosperity of both EPT and the employee. We do this through a selection process using a variety of tools and materials. Please note that some positions require more time to accomplish all of this.
The selection process may include:
What to expect:
Where to Start
To be considered for a position at any of our facilities, you must submit your resume/application electronically to a specific job posting. It is EaglePicher's policy to refuse applications submitted via other means. You must answer all questions on the application. Blank or incomplete applications may indicate you did not complete the application. The best applications are the ones that closely match the job posting. Before completing the application, highlight the relevant experience you have and customize your application to reflect it. Use the grammar and spelling checks available. Spelling and grammar errors send a message that you are not committed, not detail oriented, or have poor written communication skills.


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Severna Park, MD is filled with an eclectic array of homes, whether it's a condo or townhouse or the single-family homes found throughout the area. Just as there are a number of different types of homes, there are also many different reasons a homeowner might want to hire a cleaning service.
Whether you're a busy professional working long hours, a family looking for a bit of help with moving in/out or someone wanting cleaning services to help maintain their home, hiring a cleaning service company can make a huge difference in your quality of life. At Spouses Cleaning Houses, we know not all cleaning services offer quality work.
Spouses Cleaning Houses we take great pride in our work. We are an independently owned company offering house cleaning services on a weekly, bi-weekly or monthly basis.
Our employees are bonded and insured. All house cleaning services are fully customizable, including all rooms of the home and with flexible appointment times to suit your busy schedule. Customers can choose from regular cleaning that can include simple aspects like cobweb removal and vacuuming or deep cleaning services like scrubbing tile grout and detailed cleaning of all blinds.
Take advantage of Spouses Cleaning Houses – call us today for your free estimate.
We stand above the rest!
Want A House Cleaning Estimate?
Call (410) 571-9428 or request a house cleaning estimate online today!Request an Estimate Online
Testimonials from a Neighbor
We've used Spouses Cleaning Houses services for regular weekly cleanings for 7 years and also used them for move in/ move out services. They have been honest, reliable and provided excellent service over the years. My wife and I always admire how good the house looks when we returned after they have cleaned it!
— Solove, R., Severna Park, MD


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CM coach Alex Campea weighs in on Peter Ngobidi and his decision to attend UMass
Catholic Memorial football coach Alex Campea remembers a young ninth-grade prospective football candidate named Peter Ngobidi telling him he wanted to be a great football player.
Three years later, the 6-foot-2, 220-pounder progressed to the point where UMass offered him a scholarship. This morning, Ngobidi realized a dream when he gave a verbal commitment to play for the Minutemen in the fall of 2013.
"I'm just so happy for Peter, he's a great kid and worked very hard to get here," Campea said. "I think this is a great for Peter, he fits in very well with the schemes they are running. The coaches texted me and said they were thrilled that Peter was coming."
Despite being named to the Catholic Conference All-Star team as a junior, Ngobidi was overshadowed a bit by seniors Camren Williams and Armani Reeves, both of whom committed to Ohio State. Campea was effusive in his praise for the two Herald All-Scholastics and the way they encouraged Ngobidi.
"Cam Williams was a great teammate and Armani Reeves was a great teammate," Campea said. "They constantly told Peter to go to as many camps as possible and keep on working hard every day.
"Cam and Armani had a lot of film on them. What happened was when the colleges saw the two of them on film, they would see a No. 44 (Ngobidi) making a lot of plays on the defensive side of the ball.
"They noticed he can play."


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Stock Graphic >> Web Element - Valentines day labels and elements 3 19687 by Creative Toons @ CreativeMarket
File Size: 1.82 MB; Tileable: Yes; Requirements: Adobe CS1+; Layered: Yes; DPI: 300 DPI; Vector: Yes.
Valentine's day labels and elements Vol 3 contains floral frames, typography and decorative elements.
In this set of Valentine's day elements you will find the .jpg, .eps10 and .ai files. I've used free fonts, download links included in the zip file.
This file is layered, easy to edit text and colors and can be scalable at any dimensions without loss of resolution.
If you have any questions please feel free to contact me.
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