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PTP1B (protein tyrosine phosphatase 1B) is a negative regulator of IR (insulin receptor) activation and glucose homoeostasis, but the precise molecular mechanisms governing PTP1B substrate selectivity and the regulation of insulin signalling remain unclear. In the present study we have taken advantage of Drosophila as a model organism to establish the role of the SH3 (Src homology 3)/SH2 adaptor protein Dock (Dreadlocks) and its mammalian counterpart Nck in IR regulation by PTPs. We demonstrate that the PTP1B orthologue PTP61F dephosphorylates the Drosophila IR in S2 cells in vitro and attenuates IR-induced eye overgrowth in vivo. Our studies indicate that Dock forms a stable complex with PTP61F and that Dock/PTP61F associate with the IR in response to insulin. We report that Dock is required for effective IR dephosphorylation and inactivation by PTP61F in vitro and in vivo. Furthermore, we demonstrate that Nck interacts with PTP1B and that the Nck/PTP1B complex inducibly associates with the IR for the attenuation of IR activation in mammalian cells. Our studies reveal for the first time that the adaptor protein Dock/Nck attenuates insulin signalling by recruiting PTP61F/PTP1B to its substrate, the IR.
- Dock/Nck
- Drosophila
- insulin receptor
- insulin signalling
- protein tyrosine phosphatase 61F/protein tyrosine phosphatase 1B (PTP61F/PTP1B)
- tyrosine phosphorylation
Insulin is a key anabolic hormone that promotes the storage of fuels as macromolecules. Insulin exerts its effects by binding to its receptor, a PTK (protein tyrosine kinase) that undergoes autophosphorylation and activation and phosphorylates key substrates, including IRS [IR (insulin receptor) substrate] proteins 1–4 [1,2]. Tyrosine phosphorylation of IRS scaffold proteins allows for the nucleation of signalling complexes and the activation of key pathways, including the PI3K (phosphatidylinositol 3-kinase)/Akt pathway that elicits many of insulin's metabolic and mitogenic effects [1,2]. In mammals, a key function of insulin is to lower blood glucose levels after a meal. Insulin serves to promote glucose uptake in muscle and fat and suppress hepatic glucose production [1,2]. Drosophila melanogaster is an ideal model organism for investigating the signalling networks induced by insulin [3–6]. Indeed, many of the key pathway components and modulators of IR signalling were first described in flies. IR signalling regulates varied biological responses in flies, including longevity, female fertility and visual system formation [3–7].
PTPs (protein tyrosine phosphatases) are a large and structurally diverse family of enzymes that are integral to the spatial and temporal control of receptor PTK phosphorylation, activation and signalling [8,9]. The prototypic family member PTP1B is a negative regulator of IR activation and signalling [9]. In particular, PTP1B dephosphorylates the IR tandem Tyr1162/Tyr1163 PTK activation loop tyrosine phosphorylation site that is necessary for IR activation and signalling [10–12]. In keeping with PTP1B being an integral negative regulator of IR signalling, previous studies have shown that PTP1B-null (Ptpn1−/−) mice exhibit enhanced insulin sensitivity associated with increased IR activation in liver and muscle [12–16]. PTP1B deficiency improves glucose tolerance and delays the onset of diabetes in IRS-2-null mice [17] and in mice that are heterozygous deficient for both IR and IRS-1 [18], whereas transgenic overexpression of PTP1B in muscle promotes insulin resistance [19]. Moreover, PTP1B expression and/or activity may be increased in muscle and adipose tissue in insulin-resistant humans and rodents [20–23]. PTP1B's integral role in insulin signalling makes it an exciting therapeutic target for the alleviation of insulin resistance in Type 2 diabetes [23,24].
Although PTPs such as PTP1B display exquisite substrate selectivity in vivo, it remains incompletely understood how this selectivity is conferred. Although the catalytic domain of PTP1B has inherent specificity for tyrosyl phosphorylated substrates, additional factors contribute to substrate selectivity [9,25,26]. PTP1B is targeted to the cytoplasmic face of the ER (endoplasmic reticulum) by a hydrophobic C-terminus [27], and this subcellular compartmentalization has spatial and temporal implications for PTP1B substrate selectivity. In particular, PTP1B's location in the ER may limit access to substrates such as the EGFR (epidermal growth factor receptor) at the plasma membrane. Indeed, PTP1B is thought to efficiently dephosphorylate the tyrosine-phosphorylated EGFR only after the receptor PTK is endocytosed [28]. The non-catalytic C-terminus of PTP1B also contains a proline-rich region that allows PTP1B to interact with SH3 (Src homology 3) domains to dephosphorylate substrates such as p130cas and c-Src [29–33]. Two proline residues in the C-terminus of PTP1B interact with the SH3 domain of p130cas for efficient p130cas and c-Src dephosphorylation to regulate integrin signalling in fibroblasts in vitro [29,32,33]. Thus the proline-rich region in PTP1B may be important in conferring substrate selectivity.
The putative PTP1B orthologue in Drosophila melanogaster is PTP61F [34]. Similar to its mammalian counterpart, PTP61F is ubiquitous and expressed at all stages of development, including oogenesis and embryogenesis [35,36]. Alternative splicing of PTP61F message can result in two variants: one that is targeted similarly to PTP1B [27] to intracellular membranes, including the ER, by a hydrophobic C-terminal tail [34] and another that lacks the hydrophic C-terminus and is targeted to the nucleus by a nuclear localization sequence [34], reminiscent of mammalian TCPTP (T-cell PTP) that shares 74% catalytic domain sequence identity with PTP1B [9,37]. Hereafter, only the ER-targeted form of PTP61F will be referred to. Similar to PTP1B, PTP61F has a proline-rich sequence (five PxxP motifs) in its non-catalytic C-terminus [34]. PTP61F has been shown to interact with the adaptor protein Dock (Dreadlocks), which is composed of three SH3 domains and a single SH2 domain [38,39]. Indeed, Dock was first identified as a protein that interacted with PTP61F in a yeast two-hybrid screen [40]. This interaction was confirmed in co-immunoprecipitation experiments from Drosophila embryo extracts and Schneider II (S2) cells and shown to be mediated by one or more of the Dock SH3 domains and the PTP61F non-catalytic C-terminus [40]. Interestingly, there is also biochemical and genetic evidence that Dock interacts with the IR. Dock is required for photoreceptor-cell axon guidance and is localized to photoreceptor-cell growth cones [38]. The Drosophila IR is also enriched in photoreceptor-cell growth cones and is similarly required for photoreceptor-cell axon guidance [41]. Dock has been shown to interact genetically with the IR in photoreceptor cell axon guidance and this interaction is dependent on IR PTK activity [41]. Dock has been shown to bind to the IR C-terminal tail [41] that contains tyrosine phosphorylation sites and proline-rich sequences [41].
In the present study we assess the role of the adaptor Dock in IR regulation by PTP61F. We report the Drosophila IR is a substrate for PTP61F and that Dock is required for efficient IR dephosphorylation in vitro and in vivo. Moreover, we report that the mammalian Dock counterpart, Nck, may similarly be required for IR dephosphorylation by PTP1B. Thus our studies provide evidence that adaptor proteins play an important role in conferring PTP substrate selectivity.
Rabbit anti-Dock and mouse anti-HA (haemagglutinin; 12CA5) and anti-c-Myc (9E10) antibodies were from Abcam; recombinant human insulin, mouse anti-tubulin and anti-FLAG M2 antibodies were from Sigma–Aldrich; rabbit anti-phospho-Tyr1162/Tyr1163-IRβ antibody was from Biosource International; rabbit anti-IRβ and mouse anti-actin antibodies were from Santa Cruz Biotechnology; 21-nucleotide siRNA (small interfering RNA) duplexes to human NCK1 were from Thermo Scientific; and mouse anti-NCK1 antibody was from BD Biosciences. The mouse monoclonal anti-PTP1B antibody (FG6) was provided by Professor N.K. Tonks (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, U.S.A.) and the PTP61F antibody was provided by Professor J.E. Dixon (University of California, San Diego, La Jolla, CA, U.S.A.).
Plasmid constructs, recombinant PTP61F and substrate trapping
cDNA sequences corresponding to the full length or the C-terminally truncated [PTP61F1–339] PTP61F in the wild-type, the C237S phosphatase-dead mutant or the D203A trapping mutant form were constructed with an HA tag at the N-terminus and cloned into a pET-28a (for protein expression in Escherichia coli) or a pAc5.1A vector (for protein expression in S2 cells). cDNA sequence corresponding to full-length human NCK1 was purchased from Open Biosystems. For expression in mammalian cells, NCK1 was cloned into a pRK5 vector. Protein purification from E. coli extracts was described previously [42]. The wild-type form of PTP61F1–339 was characterized biochemically as an active phosphatase (results not shown). The in vitro substrate-trapping procedure has been described previously [42]. In brief, the HA-tagged wild-type or D203A mutant form of C-terminally truncated PTP61F recombinant protein was incubated with an aliquot of total lysate prepared from S2 cells that were stimulated with 200 nM insulin for 20 min. After incubation at 4 °C for 30 min, an aliquot of immobilized anti-HA antibody–agarose beads was added for an additional 3 h of incubation. After extensive washes, beads were boiled in SDS sample buffer, and the eluted proteins were analysed by immunoblotting with antibodies to the tandem Tyr1162/Tyr1163 phosphorylated and activated human IR β subunit [Phos-IR(Tyr1162/Tyr1163)], which also recognizes the phosphorylated and activated Drosophila IR [Phos-IR(Tyr1162/Tyr1163)].
Cell culture
Drosophila S2 cells were maintained in 1× Schneider medium supplemented with 10% FBS (fetal bovine serum) at 24 °C. For transient transfection with plasmid, Lipofectamine™ 2000 (Invitrogen) was used as a vehicle following the manufacturer's directions. For RNAi (RNA interference)-mediated knockdown in S2 cells, dsRNAs (double-stranded RNAs) of approximately 700 bp corresponding to the PTP61F and Dock genes were added to S2 cells incubated in serum-free culture medium as described previously [43,44]. After a 2 h treatment with dsRNA, cells were incubated in complete medium for 24–48 h and then processed for immunoprecipitation or immunoblotting as indicated. HEK-293 (human embryonic kidney 293) cells were routinely maintained in DMEM (Dulbecco's modified Eagle's medium) supplemented with 10% FBS. For overexpression studies, HEK-293 cells (5×105 cells/6 cm plate) were incubated with plasmid DNA (1 μg/6 cm plate) and Lipofectamine™ 2000, according to the manufacturer's directions. For RNAi studies, HEK-293 cells (5×105 cells/6 cm plate) were incubated with 100 nM GFP (green fluorescence protein; 5′-GCAGCUGACCCUGAAGUUCAU-3′) or Nck1 (5′-GAUUAUGGCUUCUGGAUGAUU-3′) siRNAs and Lipofectamine™ 2000, according to the manufacturer's directions. At 2 days after transfection, cells were deprived of serum for 6 h and then stimulated with insulin for indicated times.
Drosophila stocks and transgenics
GMR-gal4 and UAS (upstream activating sequence)-dInR strains were provided by H.E. Richardson (Peter MacCallum Cancer Center, Melbourne, Australia). UAS-Dock flies were provided by Larry Zipursky (Howard Hughes Medical Institute, University of California, Los Angeles, CA, U.S.A.) [39]. Dpp-gal4 flies were from the Bloomington Stock Centre. To generate UAS constructs for PTP61F, the coding region for PTP61F (the ER-targeted variant [34]) was amplified by RT (reverse transcription)–PCR from whole fly Canton S RNA. The fidelity of the coding region was verified by sequencing. The cDNA was cloned into the pNmyc-UAST vector (C.G. Warr, unpublished work) in-frame with the initiation codon and three copies of the Myc tag coding sequence. The resulting protein had the Myc tag at the N-terminus. Transgenic flies were generated by injection into w1118 embryos using standard procedures for P element-mediated transformation. Several independent transformant lines were established and analysed for each construct. Fly crosses were performed at 25 °C.
Drosophila eye microscopy
Flies were collected 3–5 days post-eclosion. Scanning electron microscopy was performed on a Hitachi S570 scanning electron microscope at 15 kV and ×100 magnification (Monash MicroImaging, Monash University). Images were captured using Spectrum NT software.
The IR is a substrate of PTP61F
The sequence and structural similarity between PTP61F and PTP1B [45] suggests that these phosphatases may recognize their substrates in an evolutionarily conserved manner. In mammalian cells, the IR is a bona fide substrate of PTP1B [9]. The PTP1B catalytic domain has a second phosphotyrosine-binding pocket that allows for the recognition of tandem phosphorylated substrates such as the Tyr1162/Tyr1163 phosphorylated IR [10,11,46]. Autophosphorylation of the IR activation loop Tyr1162/Tyr1163 site is required for IR activation in response to insulin [47]. The IR Tyr1162/Tyr1163 phosphorylation site is conserved in the Drosophila IR (Tyr1553/Tyr1554) and can be readily detected in Drosophila S2 cells in response to insulin with antibodies to the phosphorylation site in the human IR (see Supplementary Figure S1 at http://www.BiochemJ.org/bj/439/bj4390151add.htm). To determine if PTP61F might have the capacity to regulate the IR in Drosophila, we first determined whether the catalytic domain of PTP61F [PTP61F1–339] could dephosphorylate the Drosophila IR in vitro. Purified recombinant HA-tagged PTP61F1–339 was incubated with lysates from insulin-stimulated S2 cells and the status of IR activation was assessed with antibodies to the human IR Tyr1162/Tyr1163 phosphorylation site. HA–PTP61F1–339 readily dephosphorylated the Tyr1553/Tyr1554 phosphorylated IR in S2 cell lysates (Figure 1A). To determine whether PTP61F could act directly on the tyrosine phosphorylated IR, we took advantage of the corresponding PTP61F D203A 'substrate-trapping' mutant; PTP 'substrate-trapping' mutants have the capacity to form direct and stable complexes with tyrosine phosphorylated substrates. Purified recombinant wild-type HA–PTP61F1–339 and HA–PTP61F1–339D203A proteins were incubated with insulin-stimulated S2 cell lysates and immunoprecipitated with HA antibodies, and the associated proteins were eluted and subjected to immunoblot analysis with antibodies to the phosphorylated IR (Figure 1B). The Tyr1553/Tyr1554 tyrosine phosphorylated IR was eluted from HA–PTP61F1–339D203A, but not HA–PTP61F1–339, immunoprecipitates (Figure 1B). Therefore, these results establish PTP61F's capacity to act on Drosophila IR directly. To determine whether PTP61F regulates the IR in a cellular context, we knocked down PTP61F expression by RNAi using dsRNAs [43,44]. Insulin-induced IR Tyr1553/Tyr1554 tyrosine phosphorylation in S2 cells was significantly increased after PTP61F knockdown (Figure 1C; see also Supplementary Figure S2 at http://www.BiochemJ.org/bj/439/bj4390151add.htm). Taken together, these results indicate that the IR can serve as a bona fide substrate for PTP61F.
Overexpression of PTP61F suppresses IR-induced overgrowth in vivo
Next, we determined whether the interaction between PTP61F and the IR was of biological relevance. When the IR is overexpressed in the developing Drosophila eye using the GMR-GAL4 driver, it causes severe eye overgrowth due to hyperproliferation and increased cell size [48]. Previous studies have shown that co-expression of negative regulators of the insulin pathway can suppress this overgrowth phenotype [49]. To determine if PTP61F negatively regulates insulin signalling in vivo, we generated transgenic flies which carry the coding sequence of PTP61F under the control of the UAS regulatory sequence (UAS-PTP61F). Expression of PTP61F alone using the GMR-GAL4 driver had no effect on eye development (Figure 2). As reported previously [48], overexpression of the IR using the GMR-GAL4 driver led to a severe eye overgrowth phenotype, with substantial eye outgrowth and disorganization (Figure 2). Importantly, co-overexpression of PTP61F with the IR significantly reduced the IR-induced eye overgrowth (Figure 2). Taken together, our results indicate that PTP61F is an integral negative regulator of IR activation and signalling in flies.
Dock bridges PTP61F and the IR and promotes IR dephosphorylation
Previous studies have shown that PTP61F interacts with Dock [38–40] and that Dock interacts with the IR in flies [41]. Having established that the IR can serve as a PTP61F substrate, we next determined whether Dock might influence PTP61F's capacity to dephosphorylate the IR. First, we performed reciprocal co-immunoprecipitation experiments to confirm Dock association with PTP61F and to assess its dependence on insulin. We found that endogenous Dock in S2 cells readily associated with ectopically expressed and immunoprecipitated HA–PTP61F and this was independent of insulin stimulation (Figure 3A). Similarly, endogenous PTP61F co-precipitated with overexpressed Myc–Dock, with and without insulin stimulation (Figure 3B). Therefore Dock and PTP61F can form a stable complex and this can occur independently of insulin stimulation. We then asked whether Dock could recruit the tyrosine phosphorylated IR to the Dock–PTP61F complex for dephosphorylation by PTP61F. Myc–Dock alone or Myc–Dock and wild-type PTP61F or the phosphatase-dead 'substrate-trapping' mutant PTP61F-C237S were expressed in S2 cells. After insulin stimulation, Myc–Dock was immunoprecipitated and the level of phosphorylated IR associated with Dock, or Dock–PTP61F complexes, was examined. Tyr1553/Tyr1554 phosphorylated IR was readily detected in Myc–Dock alone precipitates (Figure 3C). HA–PTP61F also co-precipitated with Myc–Dock, but phosphorylated IR was not evident in precipitated Myc–Dock/HA–PTP61F complexes (Figure 3C). On the other hand, reproducibly greater amounts of Tyr1553/Tyr1554 phosphorylated IR were detected in Myc–Dock/HA–PTP61F-C237S complexes (Figure 3C). These results are consistent with Dock bridging PTP61F and its substrate the IR. Accordingly, we determined whether Dock could facilitate PTP-mediated IR dephosphorylation. We used RNAi to ablate Dock in S2 cells and assessed insulin-induced IR Tyr1553/Tyr1554 phosphorylation. We found that Dock knockdown significantly enhanced insulin-induced IR phosphorylation (Figure 3D). Taken together, these results indicate that Dock might serve to recruit PTP61F for the attenuation of IR phosphorylation and signalling.
To determine whether this is of biological relevance, we assessed Dock's capacity to modulate PTP61F-mediated IR regulation in the Drosophila eye. Specifically, as PTP61F had not shown a full suppression of the IR-induced eye overgrowth phenotype (Figure 2, bottom right-hand panel), we asked whether co-expression of Dock could enhance PTP61F's capacity to suppress the IR-driven eye overgrowth. As a control, we also expressed Dock on its own and Dock plus IR. Dock overexpression on its own caused a modest rough eye phenotype (see Supplementary Figure S3 at http://www.BiochemJ.org/bj/439/bj4390151add.htm) and did not suppress the IR-induced eye overgrowth (Figure 4). However, co-expression of Dock plus PTP61F, as compared with PTP61F alone, caused a further reduction in IR-induced eye overgrowth, reducing outgrowth and improving organization (Figure 4). These data are consistent with Dock facilitating PTP61F-mediated down-regulation of IR signalling in vivo.
Nck promotes IR dephosphorylation by PTP1B
The Dock protein is highly related to the mammalian adaptor protein Nck that also contains three SH3 domains and one SH2 domain [38,39,50]. In fact, human Nck rescues the dock mutant phenotype of defective photoreceptor cell axon guidance [39]. Moreover, Nck has been implicated in IR signalling [51] and shown to interact via its SH3 domain with IRS-1 [52,53], but it remains unknown if Nck interacts directly with PTP1B and facilitates PTP1B in the attenuation of IR signalling. As a first step, we determined whether Nck and PTP1B could interact in human cells. FLAG–Nck was transiently expressed in HEK-293 cells and its association with endogenous PTP1B and the IR in response to insulin assessed in FLAG or IR immunoprecipitates. Endogenous PTP1B was readily detected in FLAG–Nck immunoprecipitates with and without insulin stimulation (Figure 5A). In contrast, IR association with immunoprecipitated FLAG–Nck was only seen in response to insulin (Figure 5A). Conversely, FLAG–Nck association with the immunoprecipitated IR was increased in response to insulin (Figure 5B). These results indicate that Nck–PTP1B complexes inducibly associate with the Tyr1162/Tyr1163 phosphorylated IR.
Next, we determined whether Nck facilitates PTP1B-mediated IR dephosphorylation. To this end, we first determined whether IR phosphorylation was affected by the transient knockdown of Nck1 in HEK-293 cells using siRNAs. Knockdown of Nck1 by approximately 50% resulted in a significant increase in IR Tyr1162/Tyr1163 phosphorylation in response to insulin (Figure 5C). To complement these results, we then determined if Nck1 overexpression could attenuate insulin signalling. Transient overexpression of FLAG–Nck1 in HEK-293 cells decreased insulin-induced IR Tyr1162/Tyr1163 phosphorylation (Figure 5D). Taken together, these data demonstrate that Nck and PTP1B form a stable complex that inducibly associates with the activated IR for the attenuation of IR Tyr1162/Tyr1163 phosphorylation and signalling.
In Drosophila, the functions of the insulin-like growth factor receptor and the IR are served by a single gene, dIR, which controls diverse processes such as cellular growth and proliferation, organ size, lifespan and carbohydrate (trehalose) homoeostasis [4,6]. The IR signalling pathway is highly conserved between mammals and flies, and studies in Drosophila have defined the biological functions of several pathway components, including that of IRS-1–IRS-4, PI3K, Akt, the lipid phosphatase and PTP superfamily member PTEN (phosphatase and tensin homologue deleted on chromosome 10) and the tuberous sclerosis proteins TSC1 and TSC2 [3–7]. Mutations in genes encoding negative regulators such as PTEN and TSC1/TSC2 result in increased IR signalling and cause hyperproliferative and overgrowth phenotypes, whereas inhibiting IR signalling by mutations, expression of dominant-negatives or the overexpression of negative regulators causes decreases in cell size, cell number and organ size and promotes longevity [3,4,6,54–58]. To date, the roles of PTPs in the attenuation of insulin-induced tyrosine phosphorylation-dependent signalling in flies have remained unknown. In the present study we report for the first time that PTP61F is a negative regulator of the Drosophila IR.
Importantly, in the present study, using Drosophila as a model organism, we describe the potential for adaptor proteins to confer PTP substrate selectivity. In particular, we describe the role of the adaptor protein Dock in recruiting and facilitating PTP61F in the dephosphorylation of its substrate, the IR. We found that the expression of Dock promoted IR dephosphorylation, whereas Dock knockdown enhanced IR activation, consistent with Dock and PTP61F synergistically controlling the magnitude of the insulin response. Dock was originally identified as an adaptor for various cell surface receptors, including the IR [41], DASCAM (Down syndrome cell adhesion molecule) [59] and repulsive guidance receptor Robo (Roundabout) [60]. In general, adaptor proteins couple surface receptors to downstream effectors and function to propagate cellular signalling [39]. For example, Dock couples the IR to downstream pathway components in the developing eye and is required for R-cell axon guidance and growth cone morphology [41]. Our results define for the first time the potential for adaptor proteins to not only propagate, but also to terminate tyrosine phosphorylation-dependent cellular signalling by the co-ordinated recruitment of PTPs. Moreover, our results highlight the potential role of adaptor proteins in conferring PTP substrate selectivity. Similar to their PTK counterparts, PTPs exhibit inherent specificity for substrates [8,9]. This is exemplified by catalytic domain swapping experiments between the highly conserved PTP1B and TCPTP, and SHP-1 (SH2 domain-containing protein tyrosine phosphatase 1) and SHP-2 [25,26]. We suggest that adaptor proteins may confer additional specificity by targeting PTPs to defined substrates. It is implicit that PTPs would need to be precisely regulated in such PTP–adaptor protein–substrate complexes. One such mechanism may involve their reversible oxidation and inactivation by reactive oxygen species [61–64].
PTP61F was previously identified as a Dock-associated protein [40]. These early studies suggested that the C-terminal region of PTP61F, which contains five proline-rich motifs, may be sufficient for the association and that this may occur via one or more of the N-terminal SH3 domains of Dock [40]. As such, one may expect that the association between PTP61F and Dock would be ligand-independent, as PxxP–SH3 domain interactions are often constitutive. In the present study, we have shown that the PTP61F–Dock complex was constitutive and unperturbed by insulin stimulation. Similarly, PTP1B and Nck formed a stable complex and this too was unchanged by insulin. Although further studies are needed to define the precise residues contributing to the interaction between PTP61F/PTP1B and Dock/Nck, it is likely that the PxxP motifs in PTP61F/PTP1B and the SH3 domains in Dock/Nck play an important role. So how might Dock/Nck target PTP61F/PTP1B to the IR? It has been shown that IR in flies contains a 400-amino acid C-terminal extension, not present in the human IR [65], that contains a PxxP motif [66]. This C-terminal PxxP motif can interact with the Dock SH3 domain in a yeast two-hybrid assay [41]. Other studies have predicted an interaction between tyrosine phosphorylation sites in the Drosophila IR and the C-terminal SH2 domain of Dock [50], whereas genetic studies have suggested that there is a functional redundancy between the SH2 and SH3 domains of Dock during the process of IR-mediated R-cell axon guidance [41]. In the present study, we observed an insulin-induced association between Dock/Nck and tyrosine phosphorylated IR in S2/HEK-293 cells. Accordingly, our studies suggest that the Dock/Nck SH2 domain may play an important role in targeting Dock/Nck to the active IR. This may be particularly pertinent for the mammalian IR, which lacks a PxxP motif. It is also important to point out that the recruitment of Nck/PTP1B to the activated IR may not be direct and instead rely on an intermediate such as IRS-1. This would be in keeping with a previous study that demonstrated that the SH2 domain of Nck-2 can bind to tyrosine phosphorylated IRS-1 in response to insulin [52,53].
Irrespective of the precise molecular basis for the interactions between Dock/Nck, PTP61F/PTP1B and the IR, the present study provides insight into a novel mechanism whereby PTP substrate selectivity may be conferred. In particular, we highlight an important mechanism whereby insulin signalling may be regulated by PTP1B and define the role of the adaptor protein Nck in recruiting PTP1B to the phosphorylated IR. Interestingly, a recent study has shown that the deletion of Nck1 in high-fat fed obese mice improves glucose tolerance and insulin sensitivity [67]. Although the phenotype in Nck−/− mice was ascribed to attenuated ER stress [67], it is also possible that at least part of the phenotype may be attributed to enhanced IR signalling. The highly conserved nature of the PTP active site has presented a significant hurdle in the development of PTP1B-specific inhibitors for use in alleviating insulin resistance in Type 2 diabetes. An exciting alternative approach may be to develop inhibitors that target the interaction between Nck and PTP1B.
Chia-Lun Wu, Bree Buszard, Coral Warr, Tony Tiganis and Tzu-Ching Meng designed the research. Chia-Lun Wu, Bree Buszard, Chun-Hung Teng and Wei-Lin Chen performed the research. Chia-Lun Wu, Bree Buszard, Chun-Hung Teng, Wei-Lin Chen, Coral Warr, Tony Tiganis and Tzu-Ching Meng analysed the data. Tony Tiganis and Tzu-Ching Meng wrote the paper.
This work was supported by grants from Taiwan's National Science Council [grant number 98-2311-B-001-019-MY3 (to T.-C.M.)], the National Health and Medical Research Council (NHMRC) of Australia (to T.T.) and the Australian Research Council (to C.G.W.). T.T. is a NHMRC Principal Research Fellow.
Abbreviations: Dock, Dreadlocks; dsRNA, double-stranded RNA; EGFR, epidermal growth factor receptor; ER, endoplasmic reticulum; FBS, fetal bovine serum; GFP, green fluorescence protein; HA, haemagglutinin; HEK-293, human embryonic kidney 293; IR, insulin receptor; IRS, insulin receptor substrate; PI3K, phosphatidylinositol 3-kinase; PTK, protein tyrosine kinase; PTP, protein tyrosine phosphatase; RNAi, RNA interference; SH, Src homology; siRNA, small interfering RNA; TCPTP, T-cell protein tyrosine phosphatase; UAS, upstream activating sequence
- © The Authors Journal compilation © 2011 Biochemical Society


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I have significantly noticeable bulges beginning slightly below either side of my mouth and radiating towards my ears where my skin has been pulled. I am three weeks post surgery and still have swelling on the sides of my face.
Can a Too Tight Face Lift Improve with Time?
Doctor Answers 50
Early Facelift Swelling will go. Patience is a must!
YES. You will favorably improve with time. At 3 weeks you should look okay but you still have a lot of healing to do. Swelling takes time. Firmness takes time. Remember the healing process is defined as 1 year. Every day you will look a little better. When you get up in th morning think....its a great day and I look better than yesterday. You will. Good Luck.
Can a Too Tight Face Lift Improve with Time?
Some feeling of tightness is normal in the early days after a face lift. You will still have significant swelling and as this improves the feeling of tightness will improve with it. If you are concerned discuss it with your surgeon.
Tightness early after a Face Lift
Hi sukietawdry in Asheville,
Face lift surgery has evolved over the years, but still involves tissue repositioning and tightening. The tight sensation will resolve as the tissue relaxes. One month is still early after the face lift, and many patients report this sensation.
Yes, to a degree skin is pulled, but more importantly the tissue and muscle beneath the skin is tightened. This deeper layer is the secret to face lift surgery. The covering skin displays what is happening underneath the face. The majority of the pull is generally not on the skin layer itself.
Speak to your face lift plastic surgeon regarding any concerns you may have. Best of luck.
Dr. Chaboki
Learn more: http://www.potomacplasticsurgery.com/facial-plastic-surgery/face-lift.cfm
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Facelift swelling and tightness will go away!
Early post facelift swelling and skin tightness
That early from a facelift...you will have some swelling, so that should get better and it may be normal. Too much skin tightness after facelift, while less than ideal, will most likely "relax" with time. Sometimes, regular moisturizing of tight skin can help it feel better and adapt (strech) better.
Lines in Cheeks after Facelift
What you describe is a problem that is usually present before surgery if you search for it, but is accentuated by most facelift techniques. Fortunately, if due to skin pull, it will relax some with time. If this does change, it will take some time. I would not consider doing anything to modify this for at least 6-9 months if this does not resolve.
Resolution of tightness following a Facelift
Three weeks is still prettly early, and you can still anticipate some degree of tightness following a facelift. Most facelifts look a little tight right after the surgery, and this generally goes away as swelling resolves and your skin relaxes somewhat. This can take up to several months.
There are many different techniques used to do facelifts. Some techniques emphasize just the tightening of the skin which can result in the overly tight or overdone look following a facelift. Other techniques emphasize tightening of the deeper tissues or SMAS layer, which generally do not produce the overtightened appearance, but usually take longer, but also tend to last longer. Good results can be achieved with either when done by experienced and thoughtful plastic surgeons.
Facelift swelling and results
- There are some early changes after facelifts that do go away- bruising, swellings, some tightness, numbness. Be optimistic about improving over time!
- However, there can be some changes that can last- and occasionally a group of long folds from the area near the corner of the mouth to the outer cheek can be one of these stubborn problems.
- In patients with very loose skin, if the "pull" direction is not balanced ideally between upward and outward, surgery can accentuate or not significantly improve such an appearance. While it may improve over time--hopefully it will-- it sometimes requires a different approach. It is both a common finding and a tough one to fully correct.
- One hopes the overall result of your surgery has made nice changes that you can be happy with. Otherwise, you may need to wait 9-12 months then reevaluate your options. Best wishes.
Swelling after facelift
3 weeks after facelift surgery, one would expect to have some residual swelling. Rest assured, this will continue to decrease over the next 2 months. Many of these lines that you are seeing will improve as a result. Make sure your surgeon is certified by the American Board of Plastic Surgery.
Samer W. Cabbabe, M.D.
Board - Certified Plastic Surgeon
St. Louis Plastic Surgery Consultants
After a facelift, the face and neck can feel tight
Feelings of tightness after a facelift is not terribly uncommon. Your swelling can certainly improve over time so that you look and feel more natural.
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.

subset: commoncrawl


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A cynic would say Wednesday's Group C crunch match in Port Elizabeth pits the smallest country at this World Cup against the most deluded. Slovenia, one of those European nations who habitually show up England's technical deficiencies, plot Fabio Capello's downfall in a match that could wipe out the international careers of a generation and ignite a crisis that would make the quarter-final exits of 2002 and 2006 appear heroic.
England have been here before, in 1986, when they lost 1-0 to Portugal and drew 0-0 with Morocco before recovering to beat Poland 3-0 and reach the Hand of God quarter-final against Argentina; and again in 1990, when Bobby Robson's second World Cup team drew with the Republic of Ireland and Holland but still advanced to a semi-final against West Germany.
Both times a festival of self-laceration preceded the recovery, and this time next week you may be reading cheery news about England's second-round match in Rustenburg rather than wincing at the mess Capello's men served up against the USA and Algeria: a performance so bad, in Cape Town on Friday night, that it contained not a single redeeming feature. In its poverty of thought and movement England's display bore a kind of visceral power to shock, so the question becomes: how did they get from where they were in qualifying to where they are now, and how can they now rise to the level of Brazil or Argentina?
For the time being those questions are presumptuous because the first task is to ascend beyond the level of Slovenia, while USA and Algeria collide in the other Group C match. For once Capello made no attempt to evade reality by snorting, shuffling his feet and cuffing away questions with his usual omnipotence. His most ominous admission is that these England players are not coping "with the pressure of the World Cup" – a diagnosis that failed to impress David James, the goalkeeper, and is sure to be resented by other players, who will feel it portrays them as chokers (which is precisely how they look).
"I'm surprised, because I remember the pressure when I started. I remember the first game against Switzerland at Wembley, I remember exactly what happened," Capello said yesterday. In those early days he was convinced England's confidence had collapsed and was sure he had the cure. This was a scared team, sick of failing, weary of expectations they could never fulfil; a team, in part, that no longer wanted to be on national service.
Capello said in Cape Town: "After the performance against Croatia [a 4-1 win in Zagreb] we started to play differently, and I hope here it will be the same here.
"I can change, do different things, we have time to prepare for this really important game. Really we have to play like we do in training: passes, passes, passes, really well, really good. But the problem is not just when we have the ball, we have to win back the ball very quickly, this is one of the big problems we have now. We have to press the opponent more to win back the ball."
Steven Gerrard, the captain, said: "I agree with what the manager has said. We need to find more spirit. We weren't aggressive enough, we never pressed the ball or won it back quickly enough. We never showed enough urgency and we certainly weren't clinical enough around the box. We need to find some click for Wednesday night."
In this crushing anti-climax at the otherwise inspiring Green Point Stadium we saw confirmation that Capello and the last remnants of the so-called golden generation are pulling in opposite directions. By fiddling with the emotions of the three goalkeepers over the first two games (and dropping Robert Green when he had seemed certain to retain his place, despite the howler in Rustenburg), Capello destabilised the squad to the point where the grievances of others – Joe Cole and Matthew Upson among them – have acquired legitimacy.
A squad high on self-regard and low on achievement had never spent so much time with Capello before, and there are signs that some are fed up with his touchline tantrums and aloofness around the camp. It is classically true that characteristics previously defined as strengths are reclassified as weaknesses when results go south. Capello's boot camp no longer looks a place of much-needed correction. The players are tight and edgy. One theory doing the rounds here – and it is not as silly as it sounds – is that they should have been allowed a good night out on Saturday to loosen up, in the style of England's 2007 rugby World Cup team, after they had lost a group game to South Africa 36-0.
"It's difficult to put your finger on it right now. There's a lot of pressure on the boys, there's no lying about that," Gerrard says. "There's a lot of tension because we want to stay in this tournament for a long time. We knew that coming into it there was always going to be big pressure. You've got to play under pressure and you've got to play well.
"It's the last chance. What's done is done now. It's no good looking back, we can't change that. As captain, I've got to make sure we don't feel sorry for ourselves and go out on Wednesday night and try and perform a lot better. The fans are entitled to their opinion. Unfortunately we sent them away upset and slightly concerned."
"Slightly concerned" barely touches the fury felt by an England fan who confronted David Beckham in the England dressing room – even the Football Association's security department were off their game – and the thousands more who drew an indignant response from Wayne Rooney with their booing (Rooney apologised yesterday). "The players feel exactly like the fans. Frustrated. But at the same time we need to win our last game. There is one more game left. We need everyone behind us now," said John Terry, who was stripped of the captain's armband, and whose downfall heralded a turbulent period for Capello. "There's no point everyone criticising. We haven't done it up to now and we need to in the next game. If we do it in the next game we go through from the group."
But Capello's problems stretch beyond questions of supporter-loyalty, nerves and application. Specifically, Rooney is a ghost of his mid-season self. Incoming balls bounce off him, "he lost too many passes", to quote Capello, and the searing speed has drained from his legs. He no longer strikes the ball with the same precision or venom; he plays as if roused from hibernation. The more he fights the limitations of a tired and perhaps injured body the more doubt is cast on his chances of scoring a first World Cup goal.
Elsewhere in the team Ashley Cole is performing creditably, Jamie Carragher, who is also struggling with the speed of the game, is suspended for picking up two cautions; Aaron Lennon's courage and appetite are in doubt; Frank Lampard is becalmed and has lost the capacity to shoot accurately from long-range; Gerrard is toiling from a position too deep to advertise his talents; and Emile Heskey, though busy, looks as if he could play in four World Cups and still not score, which surely obliges Capello to play without a second striker, bring in Joe Cole at last and push Gerrard up behind Rooney to enliven a blunt attack.
England's technical failings induce deep anxiety and are alarming even the culprits. Last week Franz Beckenbauer called them a "kick and rush" team, which now sounds flattering, because kick-and-trudge would be more accurate. Terry tries to steer the conversation away from ball retention and passing to more macho matters. He said: "At times we didn't keep the ball too well. But the most frustrating thing for the manager to see was the lack of fight and desire. That's what we're all about.
"I think everyone knows we can play. Week in week out with our clubs we play football on the floor. We got criticised by a few people [Beckenbauer] during the week for playing long ball, but football wise we can mix it with anyone. But first and foremost we're a very proud country and we've got to go out there wanting to win. That, more than anything, disappointed the manager."
Terry's assertion that England can "mix it with anyone football-wise" would not bear scrutiny in a court, where the prosecution would recite their 44 years in the wilderness. It is too soon for an autopsy, but from the FA's Sir Trevor Brooking comes a bleak assessment of the talent pool just below this ageing senior squad.
"We looked at the under-21s from last summer and we promoted Joe Hart and James Milner. Adam Johnson nearly got into the squad," Brooking said. "The Germans promoted Mesut Ozil, the best player of the under-21 tournament last year, who is really talented. They had five of their players come through. It's bridging that gap."
In the next four years England may be able to summon the likes of Jack Rodwell, Nathan Delfouneso and Phil Jones, but the word is that there is no new talent wave.
"England came here with an expectation from everybody to win the World Cup. It's not easy to deal with that," Arsène Wenger, the Arsenal manager, said. He is too kind. Other countries "deal" with it just fine. Capello, who has lost his sureness of touch, looks to have met his match: a culture he seems to find bewildering.


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Goldman Sachs: What Tech Bubble?
With the Nasdaq Composite jumping to an 11-year high, the inevitable question will arise: are we back in a bubble? We asked the very same question last year around this time, and it wasn't long before the whole rally came tumbling down again.
One need look no further than Groupon and Zynga, two social-media superstars that have stumbled a bit out of the IPO gate, but which are up 19% and 33% this week alone, thanks to exuberance around the listing of some other social media fad. Does that mean there's a bubble?
Maybe not, says Goldman Sachs strategist David Kostin. In an investor note yesterday, Mr. Kostin argued that the technology sector "appears to be the most undervalued" of the 10 stock-groups on the S&P 500. He argues that investors are only paying for tech stocks to grow earnings in line with the broader S&P 500, a way more cautious stance than what they've taken over the past 10 years. The most expensive sector by this metric? Why, utilities, of course. There's your bubble.


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A look at the Star Wars: X-Wing Miniatures Game
Arriving at my FLGS at the same time as Netrunner was the new Star Wars game from FFG, the X-Wing Miniatures Game. It probably won't be much of a surprise to anyone who knows me that I had been eagerly awaiting this game. Early reports had compared it to Wings of War, but with Star Wars ships. I think WoW is a good game, but it was really the Star Wars connection that made the game interesting. I quickly picked it up, and then it had to wait as I played a lot of Netrunner first.
However, I did have time to admire the models:
The basic set comes with three ships: one X-Wing and two TIE Fighters. The base of the models had space for a cardboard insert which shows the pilot's and the ship's abilities. Two unique pilots for the X-Wing come in the basic game (Luke Skywalker and Biggs Darklighter) as well as two generic pilots (Red Sq. Pilot and Rookie Pilot). The TIE Fighters have three unique pilots (Dark Curse, Night Beast and Mauler Mithel) and three generic pilots (Academy Pilot, Obsidian Sq. Pilot and Black Sq. Pilot). Attractive cards also match each of the pilots, with the full detailing of their special abilities.
The game itself is fairly simple. It's not Wings of War, though it has some similarities.
First, each player plans their turn by selecting a manoeuvre for each ship on the special Manoeuvre dial, which is unique for each class of ship. (TIE Fighters manoeuvre differently to X-Wings).
Second, going from least skilled pilot to most skilled pilot, each player reveals the manoeuvre and uses one of the manoeuvre templates (heavy cardboard rulers) to move their ships. Little plastic 'nubs' on the bases allow accurate placing of the templates.
Following the move, the pilot can take an Action - some common ones for the starting ships are Focus, Evade, and Target Lock.
Third, going from highest skill pilot to lowest, each ship may make one attack against a ship within its arc of fire. Special attack and defence dice are rolled, the number dependent on the ship stats and range, with dodges cancelling hits. If there are any uncancelled hits, the ship takes damage. Certain results provide for critical hits, where the draw from a deck of cards shows what happens. Shields cancel hits, but when a ship take damage equal to its hull value it is destroyed and removed from the game.
Those are the basic rules of the game. Simple, huh?
The game is made more complex by the interaction of the special abilities of the pilots and the addition of special upgrade cards. Proton torpedoes and Astromech droids for the X-Wings, while skilled pilots (Luke, Mauler Mithel and the Black Squadron Pilots) can take special skills like Determination or Markmanship.
The basic set also comes with three more advanced scenarios than the basic "destroy them all" scenario:
* Political Escort, where the rebels have to escort a senator's shuttle against TIE Fighter attacks
* Asteroid Run, where the rebels must hide in an asteroid field until their ship is repaired while the Empire looks for them
* Dark Whispers, where the Empire must destroy a Rebel holonet satellite network
Constructing a squadron uses a point-based method; Luke is worth 28 points and an Academy Pilot is worth 12 points. Upgrade cards also have point values. The standard game is 100 points, but you do not have enough points to achieve that with just one starter! Luke + R2D2 + Proton Torpedoes + Markmanship is 39 points. Even two starters is not enough to play a 100 point battle.
To get 100 points worth of ships, you'll have to buy additional ships, which come in individual packages. They're quite expensive at $15 for each ship, and the first wave includes the X-Wing, Y-Wing, TIE Fighter and TIE Advanced. The second wave will include some larger ships for $30 each. That's very expensive, but it's hard to go past the Millennium Falcon.
The game itself plays well. I've played two games with the contents of just one starter, and one game with the contents of two starters (so, 2 X-Wings against 4 TIE Fighters). That last game took us only 30 minutes, so you can see that the game plays quickly. It's somewhat greedy of table space; at 100 points, FFG suggests a 3 foot by 3 foot playing space.
With just one starter, the game isn't really that interesting. You can learn the rules, but there's only so much one X-Wing can manoeuvre. Poor Luke got shot down in both of the games I played with him on his own. Once we added the second starter, the game got a lot more interesting: two X-Wings were able to work together in tandem, and that particular game against a newbie Imperial player saw each of his TIE Fighters shot down in quick succession. I don't expect the next game to be so easy!
I'm extremely pleased with the the game so far, although the cost is higher than I would like. Some of the people on BoardGameGeek.com have been very inventive in their set-ups for the games - consider this Death Star Trench, and this 800 point game. That last took 6 hours to play, which gives you an idea of the time taken by games where lots and lots of ships are on the table. I expect a 100 point game to take about 30-60 minutes.
So, those are my initial impressions of the X-Wing Miniature Game from FFG. I've got three Y-Wings, a TIE Advanced, another X-Wing and two more TIE Fighters on their way, so I should soon be able to actually play a 100 point game. If you've got any questions about the game, feel free to ask them and I'll try to give sensible answers.
Merric Blackman
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Hi Fable, I love that you boss around your very big brother!! Happy dotd!
Dog of the Day
Cat of the Day
Pet of the Day
Hi Fable, I love that you boss around your very big brother!! Happy dotd!
Hi Fable! Happy Dog of the Day! What an *enchanting* name for one enchanting, simply precious pup!
Oh yes, Fable! I certainly have enjoyed poring over your PRECIOUS pics, learning all about your happy life and just how much you mean to your family! I only wish your mom had included a pic of your wee self with your super-sized Great Dane sib! What a sight that must be! (Though I'm sure there's no question as to which one of you rules the roost!)
I'm very familiar with that "NOOO, not a bath" look, hehe, and it's no wonder you love showing off those stylin' outfits, for you make for one fabulous doggie super model! What a gem you are, Fable, a beauty inside and out, and your family SO very lucky to have a sweet and loving, smart as a whip, personality packed pup in you!:
Thanks for starting my day off with a BIG smile, sweet baby girl! Enjoy your well earned day in the spotlight, being spoiled rotten!
Lots of love, hugs and *smoochies* to you, precious Fable, and to your GD sib, too!
P.S...You with your watering can-------->♥♥♥
Star,Tigg'r , Mollie and the10 Gallon Gang!
And my Rainbow Bridge Furangels...Jingles, Cody, Fritz, Chessa, Satin, Buddy, Lizzie, Oliver, Squeaker, Moonbeam, Rosie, Ruby~
Hello Fable in Canada, You are adorable!! I enjoyed all your pics and reading about you. You sound as if you are a sweet lovebug and I laughed when I read you are boss of the Great Dane LOL. I hope all of you have many years together. Congrats on being DOTD!
Hi, Fable! I bet you and your Great Dane brother make a terrific team! I like the middle picture best -- you have such a comfortable look in that one Happy Dog of the Day to you!
I've been Boo'd ... right off the stage!
Aaahh, I have been defrosted! Thank you, Bonny and Asiel!
Brrrr, I've been Frosted! Thank you, Asiel and Pomtzu!
"That's the power of kittens (and puppies too, of course): They can reduce us to quivering masses of Jell-O in about two seconds flat and make us like it. Good thing they don't have opposable thumbs or they'd surely have taken over the world by now." -- Paul Lukas
"We consume our tomorrows fretting about our yesterdays." -- Persius, first century Roman poet
Cassie's Catster page: http://www.catster.com/cats/448678
Hello Fable,
Pleased to meet you sweetheart. Congratulations on being honored as our DOG OF THE DAY.
I loved seeing all of your pictures, even the one of you at bath time. Sending lots of love & hugs
to celebrate your special day.
I've Been Boo'd
I've been Frosted
Today is the oldest you've ever been, and the youngest you'll ever be again.
Eleanor Roosevelt
Fable is magnificent! She is certainly fulfilling her destiny as being herself!! I love boston terriers, they are so smart and loving. I digress, Fable is oneof a kind and peace and love to the whole family
Copyright © 2001-2013 Pet of the Day.com


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As FastCount continues to evolve, we are proud to offer new and innovative business opportunities to entrepreneurs looking to get involved with the data community.
Evanston, IL (PRWEB) October 31, 2012
FastCount, a compiled postal and email mailing list database headquartered in Evanston, IL, launched Private Label this month to offer business opportunity seekers the potential to operate a personalized, consumer data website. Entrepreneurs have already begun to adopt this unique opportunity to grow business and enhance their involvement with the data community.
Entrepreneurs taking advantage of this opportunity are required to find clients and guide them to the Private Label website. After that, the clients can search the database, specify a list, pay and download the list instantly.
"As FastCount continues to evolve, we are proud to offer new and innovative business opportunities to entrepreneurs looking to get involved with the data community," says Larry Organ, CEO of FastCount. "Private Label is our latest opportunity to provide rich and quality content and enhance our adopters' websites."
Lists are rented for one-time use, and multiple usage results in more revenue. Instant purchasing capabilities allow end-users to buy lists on short notice in a variety of popular consumer categories. FastCount's sister company, ConsumerBase.com, is an industry leader providing multi-channel direct marketing services
About FastCount
FastCount is a client-facing compiled postal and email sales lead database available for instant download. FastCount gives direct marketers a faster way to get leads for marketing campaign needs.
FastCount is based in Evanston, Illinois. The company was created in 2012 as a marketing tool for ConsumerBase LLC, a subsidiary of Organ Worldwide LLC.
Kathryn Fallucco, Media Editor


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Hermien Potgieter won a booking to the value of R1800 at Makarios B&B
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Four-star center Doral Moore commits to Wake Forest
Four-star center Doral Moore has committed to Wake Forest, Moore told Clint Jackson of Rivals.com.
Moore chose the Demon Deacons over offers from multiple other schools, including Kentucky, Connecticut, Ohio State and Texas.
The 6-foot-11, 230-pound center attends Montverde Academy in Montverde, Florida. He's ranked the No. 48 player in the class of 2015 by Rivals.
Moore becomes the second commitment in the 2015 class for Wake Forest, joining four-star point guard Bryant Crawford of Washington, D.C.
• HAMILTON: John Groce, Illinois continue to pursue top prospects
Wake Forest is entering its first season with former Kansas great Danny Manning as head coach after firing Jeff Bzdelik. Manning was previously the head coach at Tulsa. The program hasn't made the NCAA tournament since 2010.
The Demon Deacons have begun practice and open the season Nov. 14 against UNC-Asheville.
• HAMILTON: Trice works to stay healthy for Spartans
- Ben Estes


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These new luxury apartments offer beautifully appointed 2-Bed and 3-Bed accommodation right on the beach.
Each has a sea balcony and totally self-contained facilities for the perfect individual and family breaks.
Discover the sun, sea, surf and luxury accommodation at Tolcarne Beach Village.
As the apartments are on the beach the access road down to us is a narrow road, access to and from the beach by foot is 130 cliff steps, so you will be fit at the end of a stay with us.
Tolcarne Beach offers all round family surf fun with hire of surf equipment, wetsuits and surf lessons complete with life guard attendance during the peak season. Beach huts and deck chairs are also available for hire.
From the A30 follow the signs to Newquay.
On approaching Quintrell Downs, at the roundabout, turn right signed Porth.
Continue towards the town centre passing the surf pods on the left.
Continue along this road and you will see the Hotel Bristol on your left with a set of pedestrian traffic lights.
Turn right down towards the beach, over the crossing, between the blue iron railings. Drive right down on to the beach and you have arrived, where you will be met by one of our team.
There is parking at the foot of the cliff close to the apartments but only a narrow lane down to it, so access is only suitable for small cars.
We also have additional parking at the top of the cliff and there is nearby on the road parking and public carparks.
You can check in from 15:00. You need to check out by 10:00.


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Ordway Center for the Performing Arts generously supports MinnPost's Arts & Culture coverage. Learn why.
From plazas to profound places, architect leaves imprint
What architect made the most impact on the Twin Cities urban landscape in 2007?
Hands down, my nominee is Tom Oslund, who happens to be a landscape architect.
The seven-person Minneapolis firm of Oslund and Associates designed a major new riverfront park, took on the challenging design of the plazas outside the Twins ballpark, and is playing a leading role in the design of the new I-35W bridge, from its elegant profile to the 50-foot-high signs that will announce the Mississippi River.
In May, the 7.5 acre Gold Medal Park became Minneapolis' newest public space. The 32-foot-high mound, which is reached by a spiral walkway rising out of a green lawn filled with 300 trees, was an instant hit. The mound offers a destination, a place to view the river, to gather or to sit and reflect. The park, just east of the monumental Guthrie Theater, has given the Mill District neighborhood a much-needed green centerpiece — and the city a profound place.
Park became makeshift memorial
Within hours of the Aug. 1 collapse of the I-35W bridge, the top of the mound had become a makeshift memorial — a true sign of its success as a public place. A few weeks later, Oslund received a phone call from Linda Figg, president of Figg Engineering Group, the firm that eventually won the contract to build the replacement bridge.
"She'd gone to our website and liked the simplicity of our work," said Oslund, who advised on the pared-down concept for the bridge and became the landscape architect for the winning team. In that capacity, he played a central role at the October public workshop on the design of bridge elements. He is working on the gateway markers, lighting and the spaces at the river's edge.
Also this year, his firm was busy designing the public plazas leading to the Twins ballpark on Sixth and Seventh Streets North. Oslund's design achieves the nigh-impossible task of making the plaza inviting to both baseball fans and people just passing through the space. Oslund advised that trees planned for what is a bridge over the I-394 access lanes might survive but would not grow. Instead, he has suggested tall, vine-covered steel armatures —vertical trellises -- that will lend a sense of scale to the large open space while softening it and providing places to sit.
Awards a-plenty
Add five design awards from the Minnesota chapter (PDF) of the American Society of Landscape Architects, an honor award from the national organization for a rainwater pond at the University of Minnesota-Duluth, the design of a house and winery in California's Napa Valley and the conclusion of three years of work on the 20-acre site of the Harley-Davidson Museum in downtown Milwaukee, Wis., and it was a very good year.
"When the Harley-Davidson Museum opens in June, it will be our biggest association with a brand," said Oslund, whose work includes landscapes for General Mills and Medtronic. "General Mills is big. Medtronic is big, but this is really big."
Holiday bonuses all around!
Comments (3)
The attention paid to architecture, public spaces and the urban environment is one of the things I miss since leaving Minneapolis for Winona twelve years ago. Count your blessings.
On another note: why an accomplished landscape architect would want to be associated with urban noise pollution is a puzzle.
Tom Oslund may be a great landscape architect but his firm's web site is extremely weak. It lists words like "360 degree view" in a tag-like format but they are mere words and not links to and actual 360 degree view of the park.
I might trust his firm to build a mound of dirt for me, but I wonder about anything more intricate.
I wish the Gold Medal Park had the effect on Mpls that you see, but I just do not see it. It is too far from the "used" parts of downtown, namely the economic district. It is good to see a park go into downtown, even though it misses the mark greatly. What downtown needs is a really downtown urban space, which must be accessible to people that are actually downtown, placing it with a few blocks of Hennepin and from 4th to 7th St. I will be very surprised if this new park gets much if any use at all, until the Downtown East neighborhood fills, which is undoubtedly many years down the road.
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RESIDENTS in North Yorkshire could soon help police monitor speeding motorists in the region.
Responding to a question about monitoring speeding during a live webchat on Monday, chief constable Dave Jones said North Yorkshire Police was considering adopting a scheme which would see civilian volunteers assist police on some road safety matters.
The scheme would be based on the Speedwatch initiative, which is already under way in 20mph, 30mph and some 40mph areas in Hampshire and on the Isle of Wight, and is managed by local Safer Neighbourhood Teams but run by volunteers.
According to Hampshire Police: "Volunteers will use equipment that can monitor the speed of passing traffic and record the details, later adding them to a database.
"Vehicle checks will be undertaken by the police and letters will then be sent to the registered keepers advising them of the speed and reminding them of why it is a community concern.
"It is important to know that SpeedWatch now forms part of our response to speeding issues.
" It is the first tier in our response to dealing with this problem.
"If a problem persists, we will escalate to tier two, meaning that enforcement can be undertaken by the police Safer Neighbourhood teams."
Mr Jones said "in recognition of the concerns expressed by the community we are exploring how we can involve them more in monitoring speeding vehicles", but said the Hampshire scheme "may not be the final product in North Yorkshire".
So far, the scheme costs up to £3,000 to set up, which would have to be found by the community, either through sponsorship, parish or town councils, or community grants, and go on equipment, signs, and safety equipment.
A North Yorkshire Police spokeswoman said: "Community Speed Watch is not enforcement, but education and information gathering which could then go on to inform our enforcement activity " It's in the early stages at the moment and nothing firm has been decided.
"If a Community Speed Watch scheme is introduced, it will be in addition to, and not in place of our current enforcement strategy which uses collision data and information from the speed management protocol to determine where enforcement takes place.


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What is a meal without a dessert ? Yeah, I suppose you will survive .. I try to make a dessert at least every second day, although it seems a nice something sweet, should be eaten every day.
Not good for the waistline, though ;-) . The other night Cheri told me I have to stop now, it's not good for her figure. Right, but I'm not forcing them to have some, but of course nobody declines !
These Chocolate cups are
I serve them with unsweetened Whipped cream because the pudding is sweet, but the sauce is unbelievably nice and sweet. These delicious desserts can be served any time of the year, and just plain Vanilla ice cream is a great topping as well.
I tried to get a good photo showing the sauce at the bottom - the ramekin on the right which I took a scoop out, came out best. I used standard size ramekins and got 4 servings from this recipe, but it can also be baked in a larger dish. (Your choice)
Here's the recipe:
Bottom Sauce Chocolate Cups:
Linking up with these cool parties:


text: I will leave others to make what they will of your response about privacy. I think it is naive and inadequate. >PS: I noticed Keitai-L has no privacy policy either ;) Please feel free to take the matter up with the list owner. But since the entire list is mirrored on the web, I am puzzled as to what PRIVATE information you might think exists to have a policy about! NickReceived on Tue Dec 9 04:10:43 2003


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Quick, what do Abe Lincoln, nude body stockings, Wikipedia, George Michael, a time-traveling witch, gay cruising and a crap sandwich have in common?
Surprisingly, they're all plot points in Stephen Karam's hilarious, free-wheeling dark comedy "Speech and Debate," now in its San Diego premiere at Diversionary Theatre. The heartwarming, zippy and dance-happy play explores the inner angst of three teen misfits struggling to be noticed at their conservative Oregon high school. Brought together by loneliness, blackmail and a brewing gay sex scandal involving their drama teacher, Mr. Healy, the awkward and unlikely trio forge a lasting bond over online blogs, club meetings and bad performance art.
The three teens first meet through the cringe-worthy online blog of eccentric Diwata, a frizzy-haired, tone-deaf aspiring teen actress who's so frustrated in her failed efforts to land a part in the high school musical that she's hit the Web with her Casio keyboard to sing/speak a rumor about Mr. Healy's penchant for sex with underage boys. Tuning in that night are Solomon, the preppy 16-year-old crusading reporter for the school newspaper, and Howie, an openly gay soon-to-graduate senior who messages Diwata's blog to (sort of) confirm the rumor.
Sensing a juicy scoop, Solomon blackmails Howie into divulging the details. But because the school newspaper won't print controversial stories, Diwata convinces Solomon that the only way he'll get his story out is by helping her form a school speech and debate club. Diwata promises Solomon he can read his story at a speech contest, though secretly she plans to dump him on show day so she can perform her own kooky musical about a witch from the Salem witch trials who travels through time to rap and dance with a gay teen Lincoln.
All three teens have secrets and ulterior motives, but they share a commonality as lonely outsiders. When their fragile bond of trust is threatened by a reporter from the Oregonian newspaper who comes sniffing around for Solomon's scoop, they come together in a zany, uninhibited dance performance that celebrates their friendship and shared weirdness.
Director Jason Southerland's production is witty, fast-paced and gleeful (yes, as in "Glee" ---- these teen students break out frequently in song); and it's led with the best performance to date by up-and-coming San Diego actress Rachael VanWormer. The brainy, quirky VanWormer has played all sorts of characters at theaters around the county, but the loud, abrasive and over-the-top Diwata is a perfect match for her exuberant talents.
Kevin Koppman-Gue is all nerves, neediness and bluster as the Izod-wearing Solomon, an eager, aspiring journalist with an unfortunate character flaw that his Christian Republican parents are working hard to cure. Markuz Rodriguez is natural and confident as Howie, the disco-dancing Eagle Scout who came out to his parents at the tender age of 10. Wendy Waddell completes the cast as the school newspaper adviser and the Oregonian reporter.
There's no choreographer credited for the George Michael dance number, but it's a gas, and the comical projections by Beth Gallagher also add a lot to the story. Also worth noting is costume designer Kate Stallons, who created some inspired outfits for the frumpy Diwata.
Karam's play was inspired by a real-life political sex scandal exposed through IM chats, and there are some troubling questions in the play about the never-seen drama teacher, but "Speech and Debate" is really a sweet, uplifting story about friendship, diversity and acceptance. Audience of virtually all ages will find something to love in this oddball comedy about finding your own unique place in the world.
"Speech and Debate" *
When: 8 p.m. Thursdays-Saturdays; 2 and 7 p.m. Sundays; through April 11
Where: Diversionary Theatre, 4545 Park Blvd., San Diego
Tickets: $29-$33
Info: 619-220-0097
Web: diversionary.org
* Critic's choice


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|Dubh Linn Alma Negra Dunkel "Las Lajas"
|Dubh Linn River Moy Dark Brown Ale
|Dubh Linn Galway Bay Double IPA
|Dubh Linn Cobh IPA
deyholla (648) Minneapolis, Minnesota | July 25, 2016
An older Irish styled bar that has added a brewing capacity to serve their own beers. A dimly lit place that has a bar on one wall and a divided seating area with some pool tables in the back. Place was absolutely packed after APN. Due to this service was quite slow, probably slower than it needed to be. 7-8 beers were on tap that were average at best. Samplers were available. Food was pretty good and prices were solid. I would want to come back when less busy to get a better feel for the service but only a decent spot IMO.
Atom (227) Minneapolis, Minnesota | July 16, 2014
Larger Irish pub seems kinda like a party spot when school is in session, I heard this place gets packed. Dart boards and pool tables. I was suprised at how good the beer was actually!! Granted it's not to busy I'd go back every time I'm in town.
csaso (172) Missouri | September 10, 2013
Pretty typical Irish pub kind of place. Menu is typical of such a place but on the good side. They have 4 of their own beers on tap plus several other guest taps. Not a bad place, not great but certainly not bad. Would visit again if I am in the area.
XmnwildX12 (51) Farmington, Minnesota | August 4, 2013
Been her a couple times and both times they had the same beers. But I really dig their Potato Stout and their IPA/DIPA is not bad.
JK (413) St. Louis Park, Minnesota | September 3, 2012
A large bar and restaurant. The ambiance is a little odd, one side is a brown Irish pub, and the other is a game room with pool, darts, and more seating. About 20 taps, with four house beers when I was there. The corned beef and cabbage was good, but expensive. Not a cheap place, $50 for two entrees, four beers, and tip. Aside from their own taps, you won't any interesting beer if you are coming from the Twin Cities. Service was friendly. This appears to turn into a college watering hole late in the evening.
TheHOFF43 (169) Robbinsdale, Minnesota | August 13, 2012
What was once a favorite college drinking locale has turned into a solid brewpub. A few beers I like quite a bit and always good service and fair pricing.
BVery (668) Plymouth, Minnesota | November 17, 2011
Visited here on Nov. 17, 2011. 20 total taps, and 3 are house taps - the bartender told me that they just started brewing their own beer about 4 months ago. So I wasn't expecting a lot, but I tried the 3 house taps (which he gave me for free - hence the 10/10 on value!), which were the Pacche Radler (he tells me they wanted to make a cider, couldn't because of the laws, so this is a peach version of a beer that is close), the Maple Ale, and the Double Hop IPA. I wasn't expecting much - new to brewing, only 3 beers.....wow, was I in for a treat. All three beers were excellent, but the Double Hop IPA is downright genious. Magic in a glass, I kid you not. You really need to find a way to give this one a try - I had visions of Pliny, and I'm not joking. The bartender was so unassuming - he's like "hey, thanks for the compliment" when I was raving about the IPA, and I'm thinking "dude, no really.....do you understand what you have here...."


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I'm aware of his past.
I understand that Bobby Petrino signed a 10-year extension at Louisville in July 2006, only to leave the school for the Falcons in Jan. 2007.
I get that he left the Falcons in the middle of the following regular season at 3-10.
I know he lied about his motorcycle accident at Arkansas, subsequently leading to his dismissal from the school.
But I also saw the heartbreak from Hilltopper fans when Willie Taggart bolted for South Florida.
I also witnessed fans question the entire direction of an athletic department, watching their head coach walk out the door after the administration did everything in its power to keep him from leaving.
And that's why I'm confident in saying this is the best possible hire Western Kentucky could have made.
A good friend of mine (currently in graduate school at WKU) explained this hire in the best possible way.
"It's like the stock market. If you get a coach worth $4.5 million for $850k, you have to buy. Buy, buy, buy. It's an easy decision."
Willie Taggart took over an 0-12 program, turned it into 2-10, 7-5, and another 7-5 season that included a win over Kentucky in Lexington.
Some Hilltopper fans were saying there has NEVER been a better time to be a Western Kentucky fan.
Then Middle Tennessee and Florida Atlantic leave for Conference USA.
WKU lands its first bowl game in school history.
Then the head coach leaves before even coaching it.
A coach that told fans, "Who has it better than us?", before bolting.
It'd be an understatement to say the fan base was crushed.
But before WKU fans could even come full circle with their disappointment in Taggart, Bobby Petrino was hired.
Hello Western Kentucky, breaking news on Sportscenter.
Making a national statement
This was a significant crossing point for the future of WKU athletics.
Hire an up-and-coming assistant coach from another school, maybe an experienced head coach just looking for a school to retire at.
The kind of coach the average Sun Belt school lands.
Or...take a chance on a homerun hire?
If you're Auburn, Tennessee, Kentucky, you don't take a chance on Bobby Petrino.
As an SEC school, you've got plenty of other quality options to choose from.
Insert Mark Stoops to Kentucky, and Butch Jones to Tennessee. Hires that received rave reviews by most.
But WKU doesn't have a chance at a Mark Stoops or Butch Jones.
They did have a chance at Bobby Petrino.
On a day when Kentucky announced Neal Brown would be leaving Texas Tech to join Stoops at Kentucky as offensive coordinator, the big news was happening at Western Kentucky.
Who would have ever thought in the Bluegrass State that a coaching hire at WKU would overshadow a hire at Kentucky?
It does when you get someone the caliber of Bobby Petrino.
A win/win hire
If Petrino leads WKU to new heights, 10-2 or even 11-1 overall for two seasons and bolts, he has to pay WKU $1.2 million in six monthly payments, beginning the month after termination.
That's on top of $500,000 WKU is already receiving from South Florida for the Coach Taggart hire.
WKU gets a few winning seasons, and vital revenue for a school left behind in the conference realignment race.
In every way, this is the best possible hire Western Kentucky could make.
Was it the best possible hire Tennessee could make? Or Kentucky? No.
But for Western Kentucky, this is an out-of-the-park hire.
Petrino is so dynamic, a media member at the hiring press conference asked if Kentucky had contacted WKU about buying out of the 2013 game in Nashville, after hearing the news about Petrino.
Joking, of course.
But how many hires could a Sun Belt school make that would cause media to even joke about an SEC school buying out of a future game?
Not many.
So Hilltopper fans, especially those worried about Petrino's past---Sit back, ask yourselves this question, and get ready for a wild ride.
"Who has it better than us now?"


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The Vernal Equinox on Saturday, March 20 at 10:32am Pacific daylight time heralded the beginning of the spring season for Earth's northern hemisphere. At the instant of vernal equinox, the sun lied in the plane of Earth's equator. As a consequence, days and nights are of equal length (12 hours each) everywhere on our planet. Another consequence is that the sun rises due east along the horizon and sets due west. During the next three months, as the sun shines more and more directly on our hemisphere, daylight hours will lengthen and the rise and set positions of the sun will gradually shift toward the northeast and northwest, respectively.
Saturn lies at opposition to the sun on Sunday, March 21. Look for this creamy white planet over the east horizon as evening twilight gathers any day this week or next. Saturn remains in the sky all night currently, rising at sunset and setting at sunrise. It will remain a good telescopic target for the next three or four months. Saturn's rings are currently tilted only a few degrees to the line of sight (nearly edge-on); though they will gradually open up to nearly 30 degrees inclined some seven years from now.
The Big Dipper, an abbreviated version of the larger constellation known as Ursa Major (the Great Bear), hovers nearly straight overhead during evening hours from March through June. The seven stars of the dipper -- all but one classified as "second magnitude" in brightness -- can be distinctly seen on clear evenings, even from light-polluted city locations. The two stars at the end of the bowl of the Big Dipper point downward toward a lone, second-magnitude star: Polaris, the North Star, which perpetually marks the direction of true north.


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Presiding deity: Sri Bhagavathi Amman
Other names: Devi Kanyakumari
Thyaga: Soundary, Balasoundary
Holy Spring: Papanasa Theertham
Reputation: Southernmost boundary of India – Bharath Varsha
Speciality: Pilgrimage-cum-tourist centre
Place: Kanyakumari
Scriptural name: Kumarikandam
District: Kanyakumari
Devotees come to Kanyakumari temple with the main purpose of getting marriages happily settled in the families. Their prayers are granted by the goddess.
It is also said that those going to Kashi should visit Kanyakumari for safe results. This is a place that has great significance for a dip in the waters here so that the devotee gets relieved of his/her sins.
Sage Parasurama worshipped Goddess in Kanyakumari.
Kanyakumari is in the southernmost point of India – called Cape Comerin during the pre-independence days. The reputation of this temple is widely known to all throughout India and the world. Though the temple is on the sea shore, the water in the well in the temple is sweet and not salty.
The earlier ancient temple had been washed away in a sea erosion ( a tsunami of high intensity) and the present temple was constructed and goddess installed later, which also is over thousand years old.
Kanyakumari is the revered centre for performing rites for the ancestors. A place that has a large number of visitors from North India.
Prayer commitments:
People use to light lamps in the temple and offer new saris to the goddess besides usual abishek and archanas. People bathe in the sea and offer Tarpan to the departed ancestors for their salvation.
General information:
Kanyakumari is 25 Kms from Nagercoil, 91 from Tirunelveli and 242 from Madurai. There are convenient private and temple lodges in the place. Alaimagal lodge, Kalaimagal Lodge and Malaimagal Lodge are few to mention charging Rs. 150 a day. As Kanyakumari is an attractive tourist centre, there are also modern lodging houses the charges ranging from Rs. 150 to Rs. 800.
Those desirous to visit the temple may contact the Executive Officer on phone No. 04652 – 246223.
Probably, Kanyakumari is the only place in the world where a confluence of three seas could be seen. This is the point where the Indian Ocean in the South, the Arabian Sea in the West and the Bay of Bengal in the east meet together. The nature's beauty is in its full form here. During the full moon days, devotees and tourists can enjoy simultaneously the setting of the sun in the west and the rising of the moon in the east.
Gandhi Mandap:
In the year 1948 the ashes of the Father of the Nation Mahatma Gandhi was immersed in the sea confluence in Kanyakumari. Before immersion, homage was paid to the ashes in the pot with flowers. Gandhiji's disciple Acharya Kripalani took efforts for the construction of a memorial for him. The foundation stone was laid in 1954 and a beautiful Mandap came into being in the year 1956. It is noteworthy that sunlight falls on the Peeta in the Mandap on the 2nd October of the year, the day of Mahatmaji's birth.
Vivekananda Memorial:
There are two great rocks in the eastern direction in the sea. The bigger one measures about 3 acres and is 55 feet above the sea. There is also a symbol on this rock in the shape of a foot which devotees believe to be that of the Goddess. In the year 1892, Swami Vivekananda visited Kanyakumari, spent his time on the rock in deep medidation. A Mandap is constructed on this rock in his memory.
Aiyan Thiruvalluvar:
On the other rock, the statue of Thiruvalluvar is installed representing the minute skills in sculpture. The tall statue is very beautifully designed and stands so majestically as if conversing with tides. Boat conveyance is available to go and see the statue closely. Indeed, a pleasant wonder.
10 day Navarathri festival in the Tamil month of Purattasi (September-October). 10 day Vaikasi Visakam during May-June with a Boat Festival. The crowd will exceed ten thousands. During these festival days, the Goddess will be taken in procession both in the morning and evening. On the ninth day the Goddess will be taken round the water on the western part in a boat beautifully decorated.


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Famed West Coast rapper Game shared his thoughts on Gucci Mane's recent Twitter challenge to box any rapper who will step in the ring with him. Game says that while "Gucci's my dude," he admitted that he would get in the ring with him for charity, because it's not a negative thing. Game also reveals what he thinks would happen if Mysonne got in the ring with Gucci Mane.
EXCLUSIVE! Game: "I'd Get in the Ring & Box With Gucci Mane"
Sunny S
VladTV Staff Writer


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Contact InformationQantum Communications Corporation
1266 East Main Street, 6th Floor,
Stamford, CT 06902
Other InformationEntity Status: Active
Type of Entity: Stock
Entity Creation Date: Jun 06, 2003
Days in Business: 5223 days
Company Description
Qantum Communications Corporation is located in Stamford, Connecticut and is listed as an active Stock. Qantum Communications Corporation was started on Jun 06, 2003 which was 5223 days ago.
EmployeesFrank Washington director:
Frank D. Osborn ceo, President & Director:
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Registered AgentSecretary Of The State
30 Trinity Street,
Hartford, CT 06106-0470
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David K. K. Johnson eBooks
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Heroes and Philosophy
William Irwin & David K. Johnson & David K. K. Johnson
Wiley, July 2009
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You are here
MHS triplets embrace the differences
Ciara, Amber and Jade Collins sat silent in the car. Palpable anxiety filled the air between the triplets — a staunch contrast from the chorus of laughs, shouts and bubbly conversations that usually characterized their car rides together.
Halfway through their senior year at Malibu High School, the girls were on their way to take the ACT for the first time; a test that would make or break their college applications.
"We could all feel the tension," Jade said. "One of us turned on the radio, we cranked Two-Door Cinema Club the whole way there and just rocked out together. Even though we were so scared and nervous for that test, in that moment we were having fun just the three of us."
According to the girls, car rides are just about the only time the three of them are in the same place at the same time.
"That's where all of our moments happen — in the car, on our way to school, work, games, classes, activities," Ciara said. "I've always loved our car rides."
Ciara, "the first one born and the born leader," according to her mother, Ruth, was one of the captains of the MHS basketball team, an active member of the Youth and Government program and a member of the MHS choir. She will be attending Bard College in New York in the fall to study pre-law.
"Bard is all about outside thinking and incorporating different ideas," Ciara said. "That's the way I think — there's always multiple answers to a single question. And my whole life, with basketball and debating and choir, I feel like I've always been on stage, which I think is really good preparation for pre-law."
Amber, "the thinker" of the group, was involved in the MHS newspaper, The Current, for four years as a writer, editor, designer and eventually handling events on the business side. She also works at Bank of Books Malibu, a setting which deeply inspires all of her passions. She will be attending the University of Chicago and is considering studying philosophy or literature.
"I was drawn to Chicago because I felt like it was all about a deeper level of thinking," Amber said. "I'm also a person that can be easily inspired by my surroundings, and I can't think of a better place to get inspiration."
Jade, who is all about "creativity and optimism," has been a cheerleader for 11 years and was the captain of the MHS cheer team for two years. She is also an active artist, having just completed AP Art and designed and sewed her own dress for senior prom. She will be attending Pratt Institute in Brooklyn, New York, to study fashion design.
"It's a very small school, so you get the benefit of being surrounded by highly competitive art students," Jade said. "You still get the big city part, while still being able to experience a smaller community setting, too."
Despite harboring entirely unique personalities and being excited to experience college life separate from their sisters, the triplets do admit to one disadvantage of parting ways in the fall.
"When you're a triplet, wherever you show up, you're showing up in a crowd — you never go anywhere alone," Amber said. "We never had the experience of having to eat alone on the first day of school. So it'll all be new and exciting, but I know at some point I'll stop and look around and say, 'Where is everyone?' I'll come back with a greater appreciation for our unit."
For Ruth, the thought of sending her final three children off to college has both good and bad aspects.
"Of course, you live vicariously through them — they're all young and have got so much going on in their lives, it's been exciting to watch and to be a part of," Ruth said. "But I know now it's going to be my time to pursue some of my passions again, as well."
As they reach the end of their high school careers, Ruth has watched their individual personalities flourish. When the girls were young, Ruth said, she had them participating in all the same extracurricular activities.
"We couldn't transport them to all the different things, so if one liked gymnastics, they all had to try it," she said, laughing.
Once the girls hit fourth grade, Ruth was able to accommodate for all their different passions. She believes that shift was instrumental in developing their individual personalities, which became strong factors in each of her daughters' successes.
But, despite the recreational activities they have been involved in, Ruth has always instilled in her daughters the importance of a college education.
"I started acting and modeling very early, so I never had the chance to go to college; that's why I've always pushed them in that respect," Ruth said. "There are so many things out there to get them sidetracked, but it's all about staying focused and keeping yourself busy and having a goal in sight."
Ruth and the girls agree, though, that MHS was an instrumental key to their college acceptances.
"We could not have done any of it without Ms. Chi [MHS college and career counselor]," Ruth said. "She spent endless hours working with all the girls and myself, helping us get scholarships and telling us where to apply."
Now, with graduation recently behind them, the girls say there are plenty of things they won't regret leaving behind.
"I won't miss getting all my clothes stolen," Ciara said.
"But mom, I know you'll miss doing all those loads of wash," Amber said.
But, the triplets and Ruth know they will always been united in one way or another.
"I'm already looking for apartments in New York so I can be bi-coastal," Ruth said, laughing. "They might not like that too much, but they're too much a part of my life for them to be too far away."


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Weclome to the American Kidney Fund's Brochure Store!
One copy of each brochure is FREE!
Click here to purchase additional copies.
If you have questions or need assistance, please call our toll-free HelpLine at 1.866.300.2900 or email us at email@example.com for pricing information and assistance. Thank you!
High Blood Pressure and Your Kidneys
This brochure is important for anyone with high blood pressure. High blood pressure is the number two cause of kidney disease in the United States. But kidney problems can also cause high blood pressure. This brochure explains how the kidneys and high blood pressure are related. It also talks about what your blood pressure numbers mean and what you can do to help control them.
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Rascal Flatts A Popular Group In Albany [VIDEO]
Albany loves Rascal Flatts. So does the rest of America as far as that goes. They are Superstars in every sense of the world. We'll get to see them on January 26th at the TU Center with Sara Evans and Hunter Hayes at a 1077 'GNA Presents concert event!
Rascal Flatts puts on one of the best live shows in country music, or any kind of music to be honest. The crowd will be singing along with all those huge hits like "Life Is A Highway", "Bless The Broken Road", "Why Wait" and so many more!
Tickets are still available at the TU Center Box Office and LiveNation.com
If you want to get out of the clod make sure you vist WGNA's Facebook page for your chance to win a trip to see them in Jacksonville, Florida with airfare and hotel included plus great seats and meet and greets for 2. You can enter once a day, and its free.
Subscribe to 107.7 WGNA on