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By Paige Brady, June 4, 2009 | More posts by Paige Brady
Heads up! The new summer issue of The Whole Deal value guide is in stores now, ready to help you out with great ideas for a great summer...without great expense. Here's just a sample of what's inside:Learn to Cook, Learn to Save
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Lee Young-ae accused of defamation
Started by sChiZoO14 , Apr 12 2013 11:43 AMkorea lee young-ae
1 reply to this topic
Posted 12 April 2013 - 11:43 AM
Actress Lee Young-ae was accused of defamation by a local businessman running a food company, according to local media reports yesterday. The businessman also accused an agency that has a copyright deal with Lee.
The disputes between the businessman and Lee started in 2011 as the food company used a photo of Lee in MBC drama "Dae Jang Geum" for an advertisement.
At the time, Lee said the businessman had never signed a copyright deal on photo use and would consider legal action.
No lawsuit was filed then, but the businessman recently filed a suit against Lee saying that the actress hurt the company's brand.
Lee's legal representatives said that the agency had forged Lee's seal and illegally signed a contract with the food company.
The legal team yesterday said that it will collect necessary evidence and review whether to take legal action against the company or agency.
JoongAng Daily
Posted 12 April 2013 - 02:54 PM
The businessman used her name for his restaurant
Yet sue her for hurting the brand name, what logic is this
Yet sue her for hurting the brand name, what logic is this

subset: commoncrawl


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Quince looks like a mixture between an apple and a pear, but tastes like neither. Beneath their golden yellow skin, tough and covered in fuzz, quinces are rock-hard and unpleasantly bitter when raw. But, don't let that deter you from giving them a try. When cooked slowly with sugar, water and little lemon, they become soft and sweet and intensely fragrant with hints of jasmine, guava, pineapple and vanilla.
Today, only about 200 acres of quince trees are grown commercially in the U.S. The majority of them come from California's San Joaquin Valley (south of Fresno), where they ripen in late September and are available through November. Shopping at our farmers market, their were only four left, proving their secret popularity and possible comeback in the kitchen. Add them to homemade applesauce and serve with latkes or roast pork. Poach them in muscat wine like you would a pear, but only longer. Or, try them in an apple pie or a crumble for a new take on a old fall favorite.
Recipe: Quince and Apple Crumble
Tip: A light speckling of brown spots should not affect the fruit's flavor or quality. Store fruit that is not completely yellow at room temperature, until it has fully ripened and is deeply fragrant.


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Human-induced pluripotent stem cells (hiPSCs) can differentiate into functional cardiomyocytes (iCell Cardiomyocytes) with ion channel activities that are remarkably similar to adult cardiomyocytes. Here, we extend this characterization to cardiac ion transporters. Additionally, we document facile molecular biological manipulation of iCell Cardiomyocytes to overexpress and knockdown transporters and regulatory proteins. Na/Ca exchange (NCX1) and Na/K pump currents were recorded via patch clamp, and Na/H and Cl/OH exchanges were recorded via oscillating proton-selective microelectrodes during patch clamp. Flux densities of all transport systems are similar to those of nonrodent adult cardiomyocytes. NCX1 protein and NCX1 currents decline after NCX1 small interfering (si)RNA transfection with similar time courses (τ ≈ 2 days), and an NCX1-Halo fusion protein is internalized after its extracellular labeling by AlexaFluor488 Ligand with a similar time course. Loss of the cardiac regulatory protein phospholemman (PLM) occurs over a longer time course (τ ≈ 60 h) after PLM small interfering RNA transfection. Similar to multiple previous reports for adult cardiomyocytes, Na/K pump currents in iCell Cardiomyocytes are not enhanced by activating cAMP production with either maximal or submaximal cytoplasmic Na and using either forskolin or isoproterenol to activate adenylate cyclases. Finally, we describe Ca influx-dependent changes of iCell Cardiomyocyte capacitance (Cm). Large increases of Cm occur during Ca influx via NCX1, thereby documenting large internal membrane reserves that can fuse to the sarcolemma, and subsequent declines of Cm document active endocytic processes. Together, these results document a great potential of iCell Cardiomyocytes for both short- and long-term studies of cardiac ion transporters and their regulation.
- human-induced pluripotent stem cells
- iCell Cardiomyocytes
- ion transporters
- sodium-calcium exchange
- sodium-potassium pumps
- phospholemman
embryonic cardiac myocyte cultures have been used effectively since the 1970s for long-term studies of cardiac function and protein turnover (e.g., Refs. 22, 34, 35). Adult cardiac myocyte cultures have been used since the early 1980s with a still greater range of experimental goals (e.g., Refs. 27, 28, 32). In spite of impressive advances with a variety of cardiac myocyte cultures (17), the utility of cultured myocytes, especially adult cultures (31), has remained limited for a variety of reasons (12). In contrast to intact cardiac tissue, isolated adult cardiac myocytes are in general fragile and usually do not support sustained contractile activity. As with most differentiated cell types, extensive structural and functional changes occur during culture of adult cardiomyocytes (32). Furthermore, transfection and genetic manipulation of cultured adult cardiomyocytes are difficult. Common alternatives, such as neonatal cardiac myocyte cultures (3) and immortalized cardiomyocyte cell lines (39), allow more sustained and experimentally reliable studies. However, they also bring more equivocal relevance to physiology of the adult human heart. In this light, recent progress in generating stem cell-derived cardiomyocytes raises entirely new opportunities for basic as well as therapy-driven research (33, 42).
The minimum list of desirable cardiomyocyte culture traits for most biochemical and functional studies is extensive and challenging. For biochemical purposes, the culture must be homogeneous. To be relevant to the intact heart, the culture should form a syncytium. Further, it should express proteins that are characteristic of adult cardiac myocytes, it should be functionally stable, it should be senescent but nevertheless metabolically active, and it should allow molecular biological manipulations to overexpress and knockdown specific proteins. For studies of cardiac ion channels and transporters, cardiomyocytes must allow convenient application of patch-clamp techniques. Finally, to be directly relevant to human physiology and disease, the ideal culture will be of human origin. In this light, cardiomyocytes derived from human-induced pluripotent stem cells (hiPSCs) by a number of different approaches (11, 13, 18, 38) are increasingly attractive models.
Among the different approaches to generating hiPSC-derived myoyctes, one of the most promising uses an hiPSC line engineered to ultimately permit high volume selection for cardiomyocytes by expressing blasticidin resistance under the control of the cardiac myosin heavy chain 6 (MYH6) promoter (25). Cells derived in this way show remarkably human-like electrophysiology (25, 30). Membrane currents that have been characterized include sodium, L-type calcium, hyperpolarization-activated, transient outward potassium, inward rectifier potassium, and the rapidly and slowly activating components of delayed rectifier potassium currents (25). The potential utility of similar cultures in automated imaging platforms to characterize hypertrophic and toxicity profiles is documented (7), and the maturation of similar cultures to a nearly adult myocyte-like phenotype over several months has recently been described (16). In the present article we extend the use of hiPSC-derived myoyctes to cardiac ion transporters, including Na/K pump, Na/Ca exchange currents, Na/H exchange (NCX1), and Cl/OH exchange. In addition, we demonstrate overexpression and quantitative knockdown of selected cardiac proteins by standard molecular biological methods. The results demonstrate that this cardiomyocyte culture system has many features required for substantively improved studies of cardiac ion transporters, in particular for studies of regulatory proteins and long-term regulatory pathways that impact the expression and function of membrane transporters.
Generation of iCell Cardiomyocytes.
Functional cardiomyocytes (iCell Cardiomyocytes) were obtained from Cellular Dynamics International (Madison, WI). Generation of the hiPSC cell line was described previously in detail (25). In brief, human fibroblast cell lines were reprogrammed by retroviral expression of sox7, oct4, nanog, and lin28. hiPSC clones were then generated to exhibit blasticidin resistance under control of myosin heavy chain 6 (MYH6). A neomycin-resistance gene driven by the constitutive phosphoglycerate kinase promoter was included in the targeting vector to allow selection of genomic integrants by inclusion of neomycin in the iPSC culture medium. Neomycin-resistant iPSC colonies were subjected to genomic DNA PCR screening to identify correctly targeted homologous recombinants. hiPSC aggregates were formed from single cells and cultured in suspension. Upon observation of beating cardiac aggregates, cultures were subjected to blasticidin selection at 25 μg/ml to enrich the cardiomyocyte population. Cardiomyocyte aggregate cultures were maintained in DMEM with 10% FBS during cardiomyocyte selection through the duration of the culture before cryopreservation at 30–32 days of culture.
Cardiomyocyte cultures.
Neonatal rat cardiomyocytes were prepared essentially by an established protocol (3), and the Institutional Animal Care and Use Committee of the University of Texas Southwestern Medical Center approved the protocols employed and the experiments. The hiPSC cells (iCell Cardiomyocytes; Cellular Dynamics International) were plated to a density of 150,000–300,000/well on 0.1% gelatin-coated 12-well cell culture dishes using iCell Cardiomyocyte Plating Medium (iCPM; Cellular Dynamics International), essentially as described previously (25). For imaging and immunofluorescence studies, cells were cultured equivalently on gelatin coated coverslips. Syncitial monolayers of beating cells formed within 48 h. The iCPM medium was then removed and replaced with Maintenance Medium (iCMM; Cellular Dynamics International). Cells were then further incubated at 37°C at 7% CO2 and maintained for up to 4 wk with medium changes every 48–72 h. Transfections of DNA and small interfering (si)RNA of hiPSC cells were performed using TransIT-LT1 and siQUEST (Mirus), respectively. In the experiments using DNA transfection to express fluorescent proteins, the transfection efficiency was 15–25%. In small interfering (si)RNA knockdown experiments, siGLO Green Indicator (D-001630-01-05; Dharmacon), protein loss evaluated by Western blotting, and loss of NCX1 transport activity, all indicated that transfection was >85% efficient. Therefore, it was not necessary to use optical methods to select cells for electrophysiological measurements in those experiments. Before patch-clamp experiments, cells were removed from dishes by trypsin treatment (0.25%) and maintained thereafter in suspension at room temperature in serum-containing medium. All experiments were performed within 3 h after deplating.
Electrophysiological methods, solutions, and sphingomyelinase.
Patch-clamp and ion selective electrode methods were all described previously (8, 20, 40, 41), and all recordings were performed at a holding potential of 0 mV. In brief, the solutions employed for NCX1 and Na/K pump current measurements minimized all other currents. The extracellular solution contained the following (in mM): 115 N-methyl-d-glucamine (NMG), 4 MgCl2 or 2 MgCl2 + 3 CaCl2, 15 TEA-OH, 10 HEPES, and 0.5 EGTA, set to pH 7.0 with aspartate. The cytoplasmic solution contained the following (in mM): 80 NMG, 20 TEAOH, 15 HEPES, 40 NaOH, 0.5 MgCl2, 0.5 EGTA, 0.25 CaCl2, 8 MgATP, 2 TrisATP, and 0.2 GTP, set to pH 7.0 with aspartate. Free Ca of cytoplasmic solutions was calculated to be 0.5 μM, and free Mg was calculated to be 0.4 mM using WEBMAX EXTENDED (http://www.stanford.edu/∼cpatton/webmaxc/webmaxcE.htm). The extracellular solution for Na/H exchange measurements contained the following (in mM): 140 NaCl, 2 CaCl2, 2 MgCl2, and 0.1 Tris, set to pH 8.2, and the pipette solution contained the following (in mM): 150 KOH, 50 PIPES, 59 aspartate, 1 EGTA, 0.5 MgCl2, 8 MgATP, and 2 TrisATP, set to pH 6.8. The extracellular solution for Cl/OH exchange measurements contained the following (in mM): 140 KCl or K-sulphamate, 2 CaCl2, 2 MgCl2, and 0.1 2-(N-morpholino)ethanesulfonic acid, set to pH 6.5, and the pipette solution contained the following (in mM): 140 KOH, 50 HEPES, 59 aspartate, 1 EGTA, 0.5 MgCl2, 8 MgATP, and 2 TrisATP, set to pH 7.6 with sulfamate. All chemicals were of the highest grade available from Sigma-Aldrich. Purified Bacillus cereus sphingomyelinase (SMase) was a gift of J. Sakurai (Tokushima Bunri University and University of Tokushima, Tokushima, Japan).
Transfection and constructs.
Transfections of hiPSC cells were performed using TransIT-LT1 and siQUEST (Mirus), as noted above, following manufacturer protocols. The extracellular NCX1.1-HaloTag (Promega) construct was a generous gift of Debora Nicoll (University of California, Los Angeles) utilizing the same external glycosylation site as a pHluorin construct developed by us (20). The construct to generate an extracellular fusion of the pH sensitive fluorophore pHluorin with PLM was developed similarly to the NCX1-pHluorin fusion (20). Briefly, using site-directed mutagenesis (Stratagene), we inserted a Cla1 cleavage site just before the NH2-terminal glutamate and after the signal sequence in the canine PLM construct (gift of J. Y. Cheung, Jefferson Medical College). We then inserted the pHluorin construct as described previously (20). siRNA constructs were purchased from Dharmacon as follows: NCX, siGENOME Human SLC8A1 (M-007620-01-0005); PLM, siGENOME Human FXYD1 (M-011977-00-0005); Mock transfection, siGENOME Non-Targeting siRNA pool#2 (D-001206-14-05); and siGLO Green Indicator (D-001630-01-05) to evaluate transfection efficiencies. For electrophysiological recordings, cells were removed at selected times with Cellgro 0.25% Trypsin-EDTA (Corning). For Western blotting, cells were lysed in RIPA as described below.
Immunofluoresence imaging.
For immunofluorescence, iCells were plated into 12-well dishes containing 0.1% gelatin-coated coverslips at a density of 200,000 cardiomyocytes per well. At room temperature, cells were washed once with PBS and fixed with 4% paraformaldehyde for 15 min, rinsed two times with PBS for 5 min, and permeabilized with 0.1% Triton X-100 in PBS for 10 min. Cells were blocked for 15 min at 37°C with TBS containing 0.1% Tween and 10% FBS. Coverslips were transferred to a sealed moist box, placed on parafilm, and incubated in blocking buffer with mouse anti-sarcomeric α-actinin primary antibody (EA-53; Sigma; 1:100) overnight at 4°C. Coverslips were transferred back to the dish and washed three times with TBS-Tween for 15 min. Goat-anti-mouse secondary with Cy5 (H+L) (Jackson ImmunoResearch, 1:400) was applied in blocking buffer for 2 h at room temperature. The iCells were washed once with TBS-T, and mounted on glass slides using Vectashield mounting media with DAPI (H-1200, Vector Laboratories). Imaging was performed using a ×63 HCX Plan Apochromat, NA 1.45, glycerin lens on a Resonance Scanning Confocal Microscope (SP5; Leica). Imaging data were processed and quantified using ImageJ (National Institutes of Health).
Live-imaging and life-time analysis.
For live-imaging, iCell Cardiomyocytes were grown on gelatin-coated 12-well glass bottom culture dishes (MatTek). Cells were transfected with PLM-pHluorin or NCX1.1-HaloTag. After 4 days, confocal imaging was performed using a Nikon TE2000-U with a ×60 oil immersion, 1.45-NA objective. A 40-mW 163-CO2 laser (Spectra Physics; Newport) operating at 488 nm and 3% of maximum capacity was used for excitation. Resolution was set to 512 × 512, yielding <2-s exposure times with a pinhole of 150 μm. Cells were imaged in PBS solution buffered to a pH of 7.4 or 6.2, as indicated. For lifetime pulse-chase analysis of NCX1.1-HaloTag, cells were labeled with 1 μM of the impermeable HaloTag AlexaFluor 488 Ligand (G1002; Promega) for 15 min in culture media at 37°C. The cells were washed several times in media, and imaging was performed at selected time points, as indicated. Backgrounds were subtracted in ImageJ, and nonnuclear punctae were quantified within a binary image defined as any particle between 0.5 and 5 μm2.
Western blotting.
Cells were lysed with cold RIPA buffer (150 mM NaCl, 1% NP40, 0.5% deoxycholate, and 0.1% SDS, 50 mM Tris·HCl) containing protease inhibitor cocktail (Roche). Protein concentrations were determined by MicroBCA Protein Assay Kit (Thermo). Samples for NCX1 detection were prepared with nonreducing SDS sample buffer and incubated at 37°C for 30 min before running on a 7% SDS-PAGE gel. For PLM, reducing buffer was used and heated to 92°C for 5 min. Samples were run on a 12% SDS-PAGE gel. All proteins were then transferred onto nitrocellulose membrane overnight at 4°C. For immunodetection, proteins were probed with monoclonal R3F1 (NCX1; 1:200) and/or anti-actin (Chemicon; 1: 2,000) and polyclonal C2-PLM (1:10,000; a gift of J. Y. Cheung, Temple University). Peroxidase-conjugated anti-mouse IgG (Bio-Rad) and anti-rabbit IgG (GE Healthcare) was used as the secondary antibody at 1:3,000. Peroxidase-labeled proteins were visualized via chemiluminescence (ECL+; GE Healthcare).
Figure 1 demonstrates the filament structure of iCell Cardiomyocytes revealed by staining cells with TRITC-labeled anti-α-actinin (red in A; white in B) simultaneous with DAPI (blue in A) to localize nuclei. Actinin is clearly organized in periodic bands. From 15 measurements of segments containing 8–10 bands, the average band spacing was 2.0 ± 0.07 microns (Fig. 1C), as expected for a developed muscle phenotype. The great majority of cells are mononuclear. While these images are suggestive of a rather homogenous cell culture, the isolation of detached single cells by trypsin treatment reveals that cardiomyocytes have a substantially wide range of dimensions and morphologies. Furthermore, functional analysis of ion transporter function reveals variability that in general correlates with cell morphology.
Figure 2, A and B, shows micrographs of iCell Caridiomyocytes after deplating, together with recordings of Na/K pump and Na/Ca exchange currents. In our experience, the largest current densities were recorded in large cells that appeared "bumpy," presumably a reflection of well-developed myofilaments. As the two micrographs in Fig. 2A illustrate, the appearance of such cells ranges from a rounded form to an elongated form, more reminiscent of an adult myocyte. The micrograph in Fig. 2B shows typical small, smooth cells. With the use of 40 mM cytoplasmic Na, pump currents were activated by substituting 5 mM Na in the extracellular solution for 5 mM K, and NCX1 currents were activated by applying 3 mM Ca in the presence of 0.5 mM EGTA in the extracellular solution. For the "larger" myocytes in this dish (Cms of 100 to 250 pF), Na/K pump currents amounted to 0.98 ± 0.1 pA/pF, and peak Na/Ca exchange currents amounted to 4.5 ± 0.5 pA/pF (n = 10). The ratios of these current magnitudes are consistent with current densities in guinea pig (10, 14) or rabbit cardiomyocytes (1, 4, 37). In contrast, "smaller" iCell Cardiomyocytes (Cms of 40–100 pF) from the same dish had Na/K pump current densities of 0.7 ± 2 pA/pF and peak Na/Ca exchange current densities of 1.8 ± 0.5 pA/pF in this dish (n = 5).
Figure 2C shows the current densities of 79 iCell Cardiomyocytes from 10 different dishes, cultured for 2 to 4 wk, which served as control measurements in comparative studies. The current densities are plotted in dependence on Cm, with Cm bins defined in Fig. 2. NCX1 current density falls off below 150 pF, and Na/K pump currents fall off less steeply. Both current densities are also significantly reduced in the myocytes with the largest Cm values. Thus we avoided the largest and smallest cells in routine measurements. When Cm values nevertheless fell out of the norm, the measurements were eliminated from data sets. Accordingly, results for the six largest and eight smallest cells in Fig. 2C were discarded.
As a contrast to iCell Cardiomyocytes, Fig. 2D shows recordings of Na/K pump currents and Na/Ca exchange currents in neonatal rat cardiomyocytes using identical experimental conditions. Pump current densities were invariably <0.5 pA/pF, and we often could not resolve pump currents with confidence in relation to background and leak currents. The example in Fig. 2D is from a culture that displayed relatively large currents (0.21 ± 0.06 pA/pF; n = 6) with average Cm values of 45 ± 4 pF (n = 15). Na/Ca exchange currents never exceeded 1 pA/pF and were usually <0.5 pA/pF. Thus transporter currents in the smaller iCell Myocytes are still more adult-like than transporter currents in neonatal cardiomyocytes.
Figure 3 demonstrates the presence of proton transporters in iCells that are consistent with transporters known to exist in adult cardiac myocytes. For these recordings, we employed oscillating micro pH electrodes to detect pH gradients next to cells in the presence of a low extracellular pH buffer concentration (8, 9). As shown in Fig. 3A, Na/H exchange was detected using a cytoplasmic solution set to pH 6.8 with 40 mM HEPES and no Na, together with an extracellular solution set to pH 8.2 (0.1 mM Tris) with 140 mM Na. Upon moving the cell close to the pH electrode, an acidifying pH gradient of 0.03 pH units is detected, and the gradient under these conditions is completely abolished when the extracellular solution is switched to one with 10 μM of the Na/H exchange inhibitor EIPA (21). Under other conditions, however, substantial proton fluxes occur in iCell Cardiomyocytes by mechanisms that we did not detect in the different cell types previously employed with this method (9). Figure 3B shows that, similar to adult cardiac myocytes (29), iCell Cardiomyocytes can generate substantial proton fluxes via Cl/OH exchange. To monitor this flux in isolation, Na-free solutions were employed using K as the major cation. The cytoplasmic solution contains 1 mM Cl with sulfamate as the Cl substitute at pH 7.6, and the extracellular solution contains 140 mM KCl at pH 6.5 [0.1 mM 2-(N-morpholino)ethanesulfonic acid]. In this condition, the proton (or hydroxyl) flux magnitude, recorded as an alkalinizing pH gradient, is similar to the maximal Na/H exchange flux, and the flux is fully blocked by substituting extracellular Cl for sulfamate.
Figures 4–6 demonstrate that iCell Cardiomyocytes are readily amenable to standard molecular biological approaches to manipulate selected proteins. In Fig. 4, we demonstrate knockdown of the NCX1 Na/Ca exchanger by siRNA transfection. In the Western blot, shown in Fig. 4A, the lanes were arranged to determine NCX1 and actin densities in duplicate for 3-day mock transfections, 3-day scrambled RNAi transfections, 3-day NCX1 RNAi transfections, 5-day mock transfections, 5-day scrambled RNAi transfections, 5-day NCX1 RNAi transfections, and an NCX1 control for BHK cells that highly overexpress NCX1 (23). The duplicate measurements demonstrate >75% loss of NCX1 protein in 3 days and almost complete loss within 5 days with no change of actin as a control protein. Figure 4B demonstrates the specific loss of NCX1 currents in iCell Cardiomyocyte recordings in relation to Na/K pump currents, and Fig. 4C quantifies the functional loss of exchange current over 3 days. Figure 4D shows the time courses of loss of NCX1 protein and current. The best exponential fits give a time constant of 50 h for loss of protein and a time constant of 45 h for loss of exchange currents. Clearly, the average lifetime of exchangers must be >1 day and <3 days, and the results give no evidence for discrepancies between the total protein pool and the "functional" surface membrane pool of NCX1.
In Fig. 5, we analyze NCX1 turnover by overexpressing an exchanger fusion that can be covalently tagged on an extracellular Halo domain (19), the domain being fused to the exchanger close to the glycosylation site of the first extracellular segment of the exchanger. Exchangers were labeled with impermeable HaloTag AlexaFluor 488 Ligand (G1002, Promega) at time zero, the extracellular solution was exchanged for solution without tag, and the occurrence of green punctae within cells was quantified at the given time points subsequent to labeling. Representative micrographs of this progression are shown in Fig. 5A over 12 h after labeling cells. Figure 5B quantifies the development of internal punctae. The increase is clearly far from saturation at 12 h, and the best fit to an asymptotic exponential function gives a time constant for NCX1 internalization of 42 h. This time constant is consistent with that for loss of NCX1 protein and current, as described in Fig. 4. Although fluorescence of the Halo-tagged exchangers subsequently decreased, this decrease may arise from any of multiple chemical possibilities unrelated to exchanger degradation (e.g., fluorophore degradation). Therefore, fluorescence changes were not analyzed in detail over longer time periods.
Figure 6 demonstrates knockdown and overexpression of the sarcolemmal regulatory protein PLM (2). The Western blot shown in Fig. 6A documents first that mock transfections result in no change of PLM or actin expression over 5 days. In this blot, there is an apparent loss of protein in lane 10 vs. 4 and lane 5 vs. 11, probably related to inefficient protein transfer. Nevertheless, it is clear that after siRNA of PLM for 3 days loss of PLM amounts to only ∼60%, while protein decreases to negligible levels after siRNA transfection within the 5-day period. As shown in Fig. 6B, the best fit of PLM densities from Western blots gives a time constant of 67 h for the loss of PLM after siRNA transfection. Figure 6C demonstrates overexpression of a PLM fusion with the pH-sensitive green protein pHluorin similar to constructs developed with NCX1 (20). In this case the pHluorin was fused to the extracellular NH2-terminal of PLM. As shown in Fig. 6C, left, the expression of the PLM-pHluorin fusion appears very inhomogeneous, being distinctly concentrated in areas that possibly correspond to membrane attachment zones of the iCells to the coverslip. Regardless of the reason for the inhomogeneous expression, application of an extracellular solution with a pH of 6.2 immediately quenched the entire PLM fluorescence and washout of the acidic solution resulted in an immediate return of fluorescence to the original pattern. This result indicates that the visible PLM-pHluorin fusion protein is located entirely in the surface membrane, and the result gives no evidence for internal PLM pools in this condition.
The original goal of our experiments manipulating PLM was to demonstrate and characterize the role of PLM in regulating Na/K pump activity via the cAMP system. However, this has not been possible because we could not demonstrate a stimulatory effect of activating cAMP synthesis on pump activity. In brief, we planned our experiments carefully to be consistent with previous work using Na-sensitive dyes (5). In those experiments, cardiomyocytes were loaded with Na by removing extracellular K and thereby blocking Na/K pumps. Upon activating pumps by application of extracellular K, intracellular Na decreases more rapidly in a mid-range of Na concentrations when cAMP levels are elevated than when they are not. On this basis, activation of the cAMP system should result in an increased pump affinity for cytoplasmic Na and cause a doubling of pump activity in the mid-range of the Na activation curve. As shown in Fig. 7A, we employed a cytoplasmic Na concentration (9 mM), which is submaximal for activation of Na/K pump currents. Pump currents were activated by brief application of 5 mM K to avoid depletion of cytoplasmic Na, and we promoted adenylate cyclase activity by applying either forskolin (20 μM in Fig. 7A) or isoproterenol (0.5 μM). No change of pump current magnitudes was observed with either adenylate cyclase activating agent.
As shown in Fig. 7B, we then considered a possibility that pumps might be "preactivated" by a constitutively active cAMP system in iCell Cardiomyocytes. This possibility was consistent with the fact that pump currents often exhibited rundown during experiments. If this rundown reflected dephosphorylation of PLM, activation of cAMP synthesis would be expected to reactivate pump currents. Figure 7B shows an example in which we monitored rundown of both pump currents and exchange current. Pump current ran down by >75% over a few minutes and was not restored by application of the β-adrenergic receptor agonist isoproterenol. Outward NCX1 currents decreased, as usual, during multiple activation episodes, and this decrease was also unaffected by isoproterenol.
Finally, we used Cm recording to characterize easily interrogated properties of sarcolemma turnover in iCell Cardiomyocytes. We describe in Fig. 8 Cm changes of iCell Cardiomyocytes in response to Ca transients induced by activation of outward Na/Ca exchange current (i.e., in response to Ca influx) and in response to sphingomyelin cleavage by sphingomyelinase C. As shown in Fig. 8A, activation of outward exchange current in the presence of a low cytoplasmic concentration of EGTA (0.5 mM) results in a large increase of the iCell Cardiomyocyte Cm, roughly a 30% increase in this case over 10–15 s. The average increase for 21 measurements was 14 ± 2.2%. This pattern is typical in our experience for fibroblasts (41), and, further in this regard, we have never observed Ca-activated exocytosis of significant magnitude in isolated cardiomyocytes, including adult and neonatal cardiomyocytes from several species (20). Over time, the enhanced Cm decayed very little in most iCell Cardiomyocyte recordings. The exchange current activated by a second application of extracellular Ca was invariably decreased in magnitude, and any additional increase of Cm was relatively small. As shown in Fig. 8B, we sometimes observed that, subsequent to the exocytic phase, Cm decreased over 1–2 min substantially. This pattern, typical for fibroblasts (20), was observed rather frequently when iCell Myocytes had been cultured for >3 wk and never when cells were cultured for <2 wk. In this experiment, we also followed the magnitude of Na/K pump currents to test whether the pump is affected in a selective way by the endocytic response. The pump current decreased roughly in proportion to the loss of Cm. Similar to the exocytic responses just described, we have never observed this pattern in adult cardiomyocytes; rather, endocytosis typically occurs immediately during large Ca transients in adult cardiomyocytes and ceases rather abruptly when Ca influx is terminated (20). Finally, Fig. 8C illustrates that iCell Cardiomyocytes respond to sphyingomyelin cleavage during extracellular application of purified sphingomyelinase C (20) by massive endocytosis that constitutes >50% of the cell surface. Similar responses are observed in adult cardiomyocytes, as well as fibroblasts (20). Thus this result simply documents that the iCell sarcolemma has basic physical properties that are similar to other cell types.
We have described in this article measurements of four ion transport activities and Ca-dependent Cm changes in human-induced pluripotent stem cell-derived cardiomyocytes, thereby allowing comparisons of these cells to adult cardiac myocytes, as well as to other cells such as fibroblasts, that can be studied with the same methods. In addition, we have described basic molecular biological manipulations of iCell Cardiomyocytes to access their potential utility to study cellular regulation and turnover of ion transporters. Together, the results document that iCell Cardiomyocytes are an advantageous and promising new cardiac myocyte culture system for a wide range of ion transport studies. The results also allow us to access potential limitations of these cells for some goals. We discuss here both the overriding advantages and certain limitations of iCell Cardiomyocytes in detail.
iCell cardiomyocytes allow both acute and chronic studies of human cardiac transporters.
As illustrated in Fig. 1, the cell selection method, based on the cardiac MYC6 promoter, clearly is successful in generating a cardiomyocyte cell culture with consistent 2-μm myofilament structures and, as described previously (25), with syncytial coupling of cardiomyocytes upon expansion to confluence. As described in Fig. 2, the larger iCell Cardiomyocytes have visible features more similar to mature cardiac myocytes and consistently display Na/K pump and Na/Ca exchange current magnitudes that are equivalent to those of adult guinea pig (10, 14) or rabbit cardiomyocytes (1, 4, 37). This outcome contrasts with cultured neonatal cardiomyocytes, which, as described in Fig. 2C, have much lower Na transport activities in spite of the fact that they beat at rates (40–100/min; e.g., Ref. 24) that are comparable to those of iCell Cardiomyocytes (25). Presumably for this reason, our literature searches do not reveal any published study of Na/K pump currents in neonatal cardiac myocytes. While the implications of low ion transport capacities for neonatal cardiomyocytes are enigmatic at present, the more adult-like transport function of iCell Cardiomyocytes clearly marks them as a more advantageous cell culture system for future studies. As shown in Fig. 3, iCell Cardiomyocytes display high activities of two pH regulating systems that are characteristic of adult cardiomyocytes, Na/H exchange activity, and Cl/OH exchange (29). As illustrated in Figs. 4–6, the iCell Cardiomyocyte culture system is readily amenable to the application of standard molecular biological techniques to knockdown and overexpress individual proteins that mediate and/or regulate cardiac ion transport. Specifically, we have demonstrated nearly complete knockdown of NCX1 and PLM by siRNA, and we have demonstrated overexpression of two fluoroescent fusion proteins. These results together clearly open the way to a wide range of acute and long-term studies of human cardiac ion transport not readily possible in cardiomyocyte culture systems to date.
Membrane protein turnover rates and pathways in iCell cardiomyocytes.
In Figs. 4–6, we have presented experiments documenting that iCell Cardiomyocytes can be used to characterize the turnover of ion transporters and ion transport regulatory proteins. Figure 4 documents that siRNA transfection to knockdown the cardiac Na/Ca exchanger NCX1 results in nearly complete loss of NCX1 protein in 3–5 days, and that NCX1 function declines selectively with a similar time course. Importantly, Na/Ca exchange currents do not decline more rapidly than NCX1 protein; NCX1 current is still 65% of normal after 24 h. This result contrasts strongly to a study using antisense nucleotides to knockdown NCX1 in neonatal cardiac myocytes (6), whereby NCX1 function was lost much more rapidly than NCX1 protein. To test further whether plasmalemmal NCX1 might turn over faster than total NCX1 protein in iCell Cardiomyocytes, we overexpressed an NCX1-Halo fusion that can be labeled from the extracellular side to follow loss of NCX1 from the cell surface. This approach, described in Fig. 5, also gave no evidence that NCX1 transporters localized to the cell surface turn over faster than the total NCX1 protein pool. In contrast, the appearance of punctae in the cytoplasm, indicative of internalized NCX1 protein, occurred almost linearly over a 12-h period, and the extrapolated time constant of turnover is in the same 40- to 50-h range that we obtain for loss of total NCX1 protein and NCX1 function. That the relatively slow turnover of NCX1 in iCell Cardiomyocytes is not atypical is verified in Fig. 6 by analysis of PLM knockdown with siRNA. The loss of PLM is definitively only partial after 3 days, and it becomes nearly complete within 5 days. Thus the turnover of PLM in iCell Cardiomyocytes appears to be even longer than that of NCX1, the minimum time constant being >60 h.
Finally, we demonstrated in Fig. 6C the use of an extracellular pHluorin fusion with PLM to determine localization of an overexpressed membrane protein in iCell Cardiomyocytes. Fluorescence micrographs of the fusion protein reveal a complex pattern that alone would not allow conclusions about the cellular localization of overexpressed protein. However, the immediate loss of fluorescence upon acidifying the extracellular space to pH 6.2 demonstrates that the entire visible pHluorin-PLM fusion pool is indeed localized to the sarcolemma. The reasons why PLM expression in the cell surface is inhomogeneous are not clear at this time. Prominent PLM domains might reflect, for example, places of cardiomyocyte attachment to the coverslips, presumably dependent on integrins and cytoskeleton, or the domains might reflect other types of lateral membrane inhomogeneities, for example, the presence or more ordered versus less ordered macroscopic membrane domains. Clearly, iCell Cardiomyocytes provide a powerful new model to address questions about sarcolemmal protein localization and transporter interactions with protein networks in relation to membrane domains.
The PLM conundrum.
It is well established that PLM is a regulatory subunit of the cardiac Na/K pump, and it is widely appreciated that PLM inhibition of Na/K pumps is relieved by phosphorylation via cAMP-dependent protein kinases (PKAs) and protein kinase Cs (PKCs) (5). Nevertheless, the stimulation of Na/K pump activity by protein kinase activation is not always evident, especially not in patch-clamp experiments. As noted in results, relatively large effects of cAMP kinase activation, apparent in experiments using Na dyes to analyze pump activity (5), are consistent with cAMP kinase-dependent PLM phosphorylation increasing the apparent affinity of Na/K pumps for cytoplasmic Na. Nevertheless, using patch clamp to determine pump activity as a membrane current, multiple groups have found no evident effect of PKAs under conditions that seem directly relevant to the results with Na dyes (15, 26). It was our hope that iCell Cardiomyocytes would allow us to clearly define conditions in which activation of PKAs causes substantial stimulation of pump activity. However, we tested a range of experimental conditions using both forskolin and isoproterenol to activate adenylate cyclase and, as illustrated in Fig. 7, were unsuccessful. In addition, we eliminated a possibility that pump rundown reflects loss of PKA-dependent pump stimulation. Isoproterenol did not reactivate Na/K pump activities subsequent to pump rundown. In short, we have not resolved the long-standing conundrum that some groups observe PKA-dependent activation of cardiac Na/K pump activity, while others do not.
iCell cardiomyocyte advantages in relation to limitations for transport studies.
As documented above, the list of experimental opportunities afforded by iCell Cardiomyocytes to study cardiac ion transport is substantial: iCell Cardiomyocytes have human adult-like expression of major cardiac ion transporters, they allow long-term molecular manipulation of individual proteins and regulatory factors, and they allow analysis of transporter and regulatory protein turnover. In apposition to these benchmarks, we have tentatively encountered two limitations. First, iCell Cardiomyocytes clearly exist in a range of phenotypic states. For example, electrophysiological characterization of iCell Cardiomyocytes and other stem cell derived cardiomyocytes has shown the presence of atrial-, nodal, and ventricular-like cardiomyocytes (25, 30). Immunocytochemical characterization has also shown differential expression of cardiac markers myosin light chain (MLC)2v and MLC2a and that the expression pattern of these markers can change over time in culture (16). In our experience, clear differences in cellular morphology and membrane surface area of trypsinized iCell Cardiomyocytes correspond well to differences in transporter expression. Small cells with poorly developed myofilaments have lower NCX1 current densities and relatively low Na/K pump current densities. Current densities also are decreased in the fractions of iCell Cardiomyocytes with the largest Cms of >150 pF. The reasons for these dependencies on myocyte size are unknown at this time. Hypertrophy of cardiac myocytes can be demonstrated to cause a decrease of Na/K pump current density caused by local Na depletion during pump current measurements, rather than a genuine decrease of pump capacity (36). It remains to be tested whether a similar mechanism affects Na/K pump current measurements in iCell Cardiomyocytes in dependence on cell size. For NCX1 currents, it remains also to be tested whether the decrease of current densities in larger cells might be the result of low Ca buffering (0.5 mM EGTA) employed in the present experiments. As with adult cardiomyocytes, it can be difficult to be sure that only one cell is voltage clamped. It is readily possible that recordings with large Cms reflect the activity of two or more cells coupled by gap junctions, thereby introducing diffusional complexities. The major implication for future experiments is that iCell Cardiomyocytes must be selected carefully to be comparable. This can best be accomplished objectively by performing comparative experiments in a blinded fashion.
Second, the Cm responses of iCell Cardiomyocytes to Ca transients, as described in Fig. 8, are similar to responses that we commonly observed in fibroblasts and HEK293 cells (20, 41), rather than adult cardiomyocytes (20). Many explanations of these results are possible at this time. One possibility is that the growth of cardiomyocytes on dishes, which promotes a flattened morphology, versus within a three-dimensional matrix in situ affects membrane trafficking and recycling mechanisms. Alternatively, acutely isolated adult cardiac myocytes may have significantly altered membrane cycling properties as a result of isolation procedures, and their function in these types of experiments may not be representative of cardiomyocytes in situ. A third possibility, which cannot be eliminated at this time, is that iCell Cardiomyocytes might retain some fibroblast-like properties that affect how membrane is trafficked and recycles.
In summary, the potential limitations of iCell Cardiomyocytes for studies of cardiac ion transporters and their regulation are significant. However, these limitations also raise new possibilities to address important open questions about the long-term regulation of cardiac ion transporters and the function of the cardiac secretory pathway, potentially in relation to cardiomyocyte differentiation. Beyond reasonable doubt, iCell Cardiomyocytes allow a wide range of new cellular and molecular experimentation, not previously possible, to address the molecular function and regulation of cardiac ion transporters.
This work was supported by National Heart, Lung, and Blood Institute Grants HL-067942 and HL-513225 (to D. W. Hilgemann).
No conflicts of interest, financial or otherwise, are declared by the author(s).
Author contributions: M.F., F.-M.L., M.-J.L., O.W.M., H.-R.W., and D.W.H. conception and design of research; M.F., F.-M.L., M.-J.L., H.-R.W., and D.W.H. performed experiments; M.F., F.-M.L., M.-J.L., H.-R.W., and D.W.H. analyzed data; M.F., F.-M.L., M.-J.L., O.W.M., and D.W.H. interpreted results of experiments; M.F., F.-M.L., M.-J.L., and D.W.H. prepared figures; M.F. and D.W.H. drafted manuscript; M.F. and D.W.H. edited and revised manuscript; M.F., F.-M.L., M.-J.L., O.W.M., H.-R.W., and D.W.H. approved final version of manuscript.
We thank Blake Anson (Cellular Dynamics International) for critical discussions and helpful advice.
- Copyright © 2013 the American Physiological Society


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Oh hi! So, this used to be my account a very long time ago, until I gained some common sense and changed my user to @something1d (bc harrygimmegravy? like really what was I thinking)
If you want to read my stories, please check them out through my profile here:
I'm not going to be checking/updating/looking at this account at ALL, so if you ever want to talk to me or anything, do it through @something1d please! I have no clue if anyone will ever even look at this account, so there's probably going to be absolutely no one on here who will ever read this and I'll just seem ridiculous hahaha oh god. Alright.
Thank you, love you very much :) <3
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text:
- Released: Oct 31, 2005
- Sound: 9
- Lyrics: 9
- Overall Impression: 10
Users' score:
(2 votes)
Sound — 9
Kid Courageous is the hottest new punk band out of Australia, this EP is thier best released work so far. Having Mick on lead vocals and Keys gives them an extremely unique sound for a punk band, Soon as I heard some of their work I was addicted and needed more. This is their third CD they have released, their debute album is to be released early 2006.
Lyrics — 9
The lyrics are quite diverse going from deep and meaningfull about girls from far away, to silly songs (Use Me) about kissing girls in calss. Mick a classicaly trained pianist and singer dose an excellent job on this EP, hitting very high long notes perfectly. Outstanding work.
Overall Impression — 10
I honestly cant compare it to any other artists work, it is so brilliant and unique. The most impressive song would have to be "About A Pretty Girl From A Town Far Away" a slow piano song that is just amazing. I love everything about it, if it was stolen I would buy 5 more, excellent price best 5$ I have ever spent!


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We should replace all negatively connotative words such as fat, large and obese with awesome words like voluptuous :D
CrazyHappySad 31-35, F 5 Answers 5 Sep 9, 2011
What would we replace "unhealthy" or "underweight" with? Is this some sort of new political correctness?
Best Answer
We don't need to replace any words.<br />
Connotation derives from context and tone.
Best Answer
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Why is supporting smoking bans, trans fat bans, large soda bans and legalizing marijuana part of the same ideology?It doesnt make sense that the same group of people supporting a total smoking ban for bars and cigar shops, trans fat bans, large soda bans also support legalizing pot gummy bears...BurroBonton 26-30, M 8 Answers 2 May 25, 2015 in Politics
Is it contradictory to support banning smoking in bars, banning trans fat, banning large sodas, banning happy meal toys.and legalizing marijuana?


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Saturday is coming up soon, so that means another race day. This week is the Jet to Jetty 5K.
When I laid out my race calendar, this was the target race for the Summer of Speed. It is a fast course that I have run many times. I figured I would have plenty of speed work under my belt by now, and this is the race where I would go for that elusive sub-20. Last year I ran two 5Ks at 20:06 but couldn't quite dip to 19:xx for the first time in over 17 years.
Well, July was a good month with plenty of interval work. But August has been kind of a bust, when a cutback week for travel turned into 3 weeks without any speed work after I had a little muscle strain. And my tune-up 5K from two weeks ago turned into a frustrating 2.4K disaster.
After that muscle strain, I took 6 days off. Since then I did 7 miles Sunday, 5 miles Tuesday and 6 miles at the track yesterday. The muscle seems to be OK, just a little tight, so that's good. But my overall feel has been sluggish. Just don't feel 100%.
Still, my times at the track were still good. With the race coming up, I cut the workout short - 4 400s and 1 800.
400 at 10K pace: 1:41
400 at 5K pace: 1:36
400 at mile pace: 1:27
400 at mile pace: 1:26
800 at 5K pace: 3:15
So, I am going to shoot for sub-20, but I feel like I'm not quite ready. If I get under 20:30 I will be happy, and I have another fast course 5K on tap for Sept 22nd to really go for it! Plus I will get to meet up with loopsters Atom Buddy and RunningBuddyMark. And there should be an In 'N Out Burger truck there for post-race chowing.


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Country Casual Teak - Premium Teak Furniture
Country Casual Teak - Premium Teak Furniture
- Country Casual Teak outdoor furniture
Since 1977, Country Casual Teak has provided the highest quality of premium teak patio furniture to hundreds of satisfied customers all over the Los Angeles area. We offer a variety of teak outdoor furniture for both residential and commercial applications. Teak's weatherproof qualities make it the ideal choice for Los Angeles outdoor teak furniture and many pieces of classic teak furniture are still in use after over 70 years. Teak's contains a very large amount of oil and rubber, making it virtually immune to Los Angeles's environmental factors. In fact, teak's weatherproof qualities made it the first choice for British sailing vessels in the Atlantic. Our patio furniture has flourished in the Los Angeles area and continues to be the premier choice for Los Angeles outdoor furniture for hotels, clubs, restaurants, parks, and homes.
- The best choice for Los Angeles patio furniture
Country Casual Teak's outdoor furniture exclusive designs have highly refined elements to ensure the highest level of comfort while still maintaining optimum strength and durability. We offer a wide range of outdoor furniture options ranging from teak Adirondack chairs, chaise lounges, teak benches, umbrellas, dining tables, rocking chairs and more. Also, we can personalize any of your teak patio furniture with custom wood engraving.
- Shop for Outdoor Furniture online or by phone
Our eco-friendly teak is a favorite of Los Angeles furniture buyers. Further, each piece of our teak furniture conforms to our strict standards of quality and embodies our environmental stewardship. Country Casual Teak is committed to renewable resources and environmentally conscious business practices. We require that only ecologically grown and harvested timbers from managed, sustainable forests be used in the manufacturing of our teak outdoor furnishings. We strive to use recycled papers and cardboard in packaging and reduce waste in business operations and to provide all residents of Los Angeles furniture that they can feel proud to own.


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BALLSTON SPA - A Ballston Spa man pleaded guilty Monday to beating a Greenfield man on Sept. 7, 2002, near a bar on Caroline Street in Saratoga Springs.
Michael J. Mosley, 33, of 17 Ballston Ave., Ballston Spa, will be sentenced Sept. 22 to six months in Saratoga County Jail and five years of probation, Assistant District Attorney Richard Wendling said.
Mosley is currently serving a year in county jail on an unrelated drunken-driving charge. He will serve the sentences consecutively, Wendling said. Mosley pleaded Monday morning just before jury selection in his trial on the assault charge was supposed to begin.
The victim, 26-year-old Robert Do of Greenfield Center, suffered a serious injury to his head and required a week in a neurological intensive care unit. Do recovered over time.
"I spoke with the victim, who seems to be satisfied," Wendling said. "I think it is a satisfactory disposition, too."
Wendling's boss, Saratoga County District Attorney James A. Murphy III, said he accepted the plea agreement because there were problems with the prosecution's case and he wanted to make sure Mosley was convicted of a felony.
At first, police charged Mosley's brother, 24-year-old Arthur Mosley of 15 Ballston Ave., with felony assault and didn't charge Michael Mosley with anything. Grand jurors charged Michael Mosley with the felony and downgraded charges against Arthur to a misdemeanor charge, Murphy said.
The misdemeanor assault charge against Arthur Mosley is still pending, Murphy said.
"That, coupled with the fact that the victim remembers nothing of the incident, meant we had a case where the only witnesses were defense witnesses," Murphy said.
Murphy also said the five-year probation sentence is significant for Michael Mosley.
"He basically has to walk the straight and narrow for the next five years," Murphy said.


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Trackside Support
The TMPP Support Truck is on the Road and at the Races!
As a racing-industry leader, Tim McAmis Performance Parts provides racers with trackside support like no other. Working out of a state-of-the-art mobile performance warehouse and fabrication shop, the TMPP Trackside Support Team is able to assist racers with any kind of issue that may arise during an event.
From nuts, bolts, hoses and fittings to safety products, hood scoops and electronics, Tim McAmis Performance Parts has thousands of replacement products on-site while also arranging for free delivery of orders to each race. Along with VP Racing Fuels, LAT oils and lubricants, a Co2 filling station and shock dyno, TMPP Trackside Support keeps you going rounds by supplying service you will not find anywhere else.
When you need it most, the highly experienced and qualified TMRC craftsman provide welding, fabrication and expert composite repair. Additionally, certified technicians from Racepak, MSD, Davis Technologies and NW Radio can be found at the TMPP Trackside Support transporter to ensure that you are trouble-free when it comes time to pull into the water box.


text:
By Gennifer Biggs, Business Solutions magazine.
It is a question heard at most recent industry events and partner summits: How do I, as a VAR or managed services provider (MSP), participate in cloud services? The concern of solutions providers is that, with cloud solutions rolling out to resolve IT concerns ranging from email security to disaster recovery, there is little left for them to offer. "We hear from our partners that they are afraid of getting left out when it comes to cloud solutions," says Lee Garrison, CMO at KineticD, formerly Data Deposit Box, a hosted backup and disaster recovery solutions vendor. "Quite the contrary, we feel there is a big opportunity for resellers, particularly with SMB customers, who are rapidly adopting the cloud." Why? Garrison explains that not only are the value propositions of cloud — enterprise-class solutions at affordable subscription pricing that eliminates the need for upgraded hardware, new software licensing, and in-house IT support — particularly appealing to SMB customers, those companies are also a perfect fit for MSPs and VARs because they usually have no IT staff. "Plus, the same advantages of cloud that SMBs enjoy also apply to the resellers," explains Garrison. "VARs can offer an enterprise-class cloud service without upfront investment in equipment or technicians, and that means they can quickly participate in the recurring revenue model and get their margin, yet the provider does all the work."


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Like those above, I like the idea. I think all you K-12s have the jump on us profs in technology sometimes–I've learned a lot by slicing this month and now here's one more new thing to add.
I had a hard time reading it because of the orientation, but just the same, I appreciate the effort. (And I'm glad that you are "crawling back" to feeling like a human again–yay!)
sorry to have missed you last weekend. Also, sorry you were under the weather. We had a great time and loved every minute of it. Hope to blog about it soon!
Is there a way to rotate the image? I can't seem to find one.


text:
the new phone, known as the sgh-i310, is powered by windows mobile 5.0 for smartphone. usb 2.0 connectivity is also provided, which allows the phone to be utilized as a removable hard disk. other features include a microsd slot, tv output, and a two-megapixel digital camera with flash. to enhance the phone's music capability, it comes with dual speakers, a digital power amp, and support for bluetooth stereo, which allows music to be transmitted to other bluetooth devices over the air.
the phone is expected to launch in europe during the second half of this year.
samsung may be on to a winning formula by combining the storage capacity of popular music players with a phone and a digital camera. the key to a true ipod killer may be convergence. if you can get the same great features of three individual devices all in one device, you may have something.
of course, with convergence comes a premium in price. and then it all comes down to what people are willing to pay for.
read more from the samsung press release.
user comments 4 comment(s)
|huh (9:18am est tue mar 07 2006)
cool. hope has fast processor. add some emulators to it. hack it. – by bla
|cool… (12:15pm est tue mar 07 2006)
soon we will be able to stick the phones on our heads and record our whole lives and make it available for download to the next generation. – by truegamer
|specs (1:37pm est tue mar 07 2006)
how come the specs never mention battery performance?
– by curious
|apple samsung (5:21pm est tue mar 07 2006)
a file leaked out in japan saying that samsung is releasing a 30 gb ipod wanna be cell phone. it will be pretty much just like the ipod except it will play avi and windows media player movies. it will also play mp3 and ogg files. – by insider


text:
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Drama for people with special needs
Publisher: A&C Black
ISBN10: 0713643846 ISBN13: 9780713643848 LCC: RC489 Edition: (UK)
Includes bibliographical references and index.
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- Physical Description: iv, 172 p. ; 22 cm.
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text:
Rugby Stripe Applique Pillow Covers
No longer available
- Overview
After an intense game on the field or television, rest your weary head on these classic rugby-stripe pillow covers.
- 16" square
- 100% cotton twill.
- Zipper closure.
- Insert sold separately.
- Personalize it with your initials or favorite number in orange, navy or charcoal applique.
- Monogramming is included at no additional charge.
- Machine wash.
- Imported.
Mix + match our bedding to create your own unique look with our Design Your Own Bed Tool.
- Dimensions + Care
- Machine wash in warm water, gentle cycle.
- Use only nonchlorine bleach, if needed.
- Tumble dry on low heat; remove promptly.
- Warm iron if needed.
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Charcoal/Black Pillow Cover
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text:
The Quality Cook Book
The front panel is green with green ink. There is a skillet with four wavy lines representing fumes rising from it. Roberts & Mander Stove Co. Gas Range Division is underneath it. The back panel is plain. In the lower left of page one is Form 118. Pages 1-4 deal with The oven heat control. Page 5 is a chart on measures and weights. Pages 6-7 have oven cooked dinner ideas. The rest of the leaflet is menus. There is a four page glossy insert in the center with descriptions and drawings of stoves. Pages 31-32 have instructions on cold pack canning.
Donor: Sliker, Shirley Brocker
Brand Name: Roberts & Mander Stove
Publisher: Roberts & Mander Stove Co. (Philadelphia, Pa.)
1930 (ca.)
32 p.
17.8 x 12.4 cm
Language: English
Key Terms:
Copyright: Copyright owner/holder cannot be located. This item is made available strictly for educational and research purposes. If you have any more information about this item or if you are the copyright owner and believe our website has not properly attributed you, please let us know.
Citation: The Quality Cook Book. The Alan and Shirley Brocker Sliker Collection, MSS 314, Special Collections, Michigan State University Libraries. Available at http://www.lib.msu.edu/exhibits/sliker/detail.jsp?id=3383


text:
West facing window in North Texas. The sun is so hot it's burning blinds! Imagine the fade damage inside. Avoid this issue with window film that blocks harmful UVB and UVA rays.
Views: 220
One of those halogen free standing lamps hugging the blinds inside?
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text:
Auburn focuses on SEC -- not national -- title
Updated: September 13, 2003, 9:07 PM ETBy Pat Forde | Special to ESPN.com
Welcome to Auburn Football Jeopardy. The category: Offensive calamities. The answer: 1967. The question: When was the last time the Tigers scored as little as three total points in consecutive games? Yeah, it's that bad for a program that began the season in everyone's top five. Ask former offensive coordinator/current Louisville head coach Bobby Petrino about the Auburn implosion and he just grimaces and tries to avoid the question. When pressed he says, "Yeah, I'm surprised about it. You never know why things like that happen, but they'll be all right. They've got good coaches and good players." It's pretty much too late to be all right by preseason-expectation standards, but Petrino is saying what he needs to say for a fan base that misses his play-calling and game planning acumen. A few Auburn fans have been e-mailing the Louisville football office requesting Petrino's return to The Plains as soon as possible.
No, the Tigers offense is not breaking big plays. Auburn's longest run: 17 yards. Auburn's longest pass: 30 yards. Both are the shortest of any SEC team, and the Tigers are last in the league in passing efficiency. But the defense is not setting up any scores with turnovers. Through two games Auburn has just one forced fumble and no interceptions -- and clearly, this is an offense that could use a couple of short fields to get itself jump-started. "We just have not played well on either side of the ball," coach Tommy Tuberville said. "Not just on offense."
The Tigers open SEC play against league punching bag Vanderbilt this week, but Tuberville is hoping his team assumes the underdog stance. "We've been so good the last few years playing the role of underdog," he said. "Obviously, we did not handle this (the big-dog role) very well. ... We still have an opportunity to get back to Atlanta (for the SEC title game). We just better wake up. ... This still is the best team I've had, potentially, in the last nine years (as a head coach)."
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text:
fix (1)
This page is about the slang term fix (1)
Meaning: to influence the result of a contest, a vote, or a race by illegal or unethical means
For example:
- The result of the football game was fixed by a group of gamblers who bribed the referee to make decisions that favoured one team.
- The fixing of matches, or match-fixing, has been a problem in sports such as boxing and soccer.
Quick Quiz:
The judges who were involved in fixing the boxing match were
This entry is in the following categories:
Contributor: Matt Errey


text:
The K99 Greeley Stampede Concerts- Rodney Atkins!
It's Memorial Day Weekend- the unofficial kickoff to summer, and with that–
K99 presents The Greeley Stampede Concerts this summer! Kicking things off– Rodney Atkins with Little Big Town on Friday June 24th!
Rodney seems to have a way at bringing out the patriotism in me. His songs really seem to celebrate living in this great country!
The song that really says: "USA! USA! USA!" is of course "It's America".
His concert on Friday June 24th at The Greeley Stampede– Presented by K99 and The Colorado Beef Council is going to be fantastic!
Get your tickets today- they start at only $24! Get details HERE!
Subscribe to K99 on


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Longtime Lions coach decides to step down
ST. LEO - In the same blunt, unbending and unforgiving fashion with which he has always criticized himself and his team during a trying 10 years as Saint Leo men's basketball coach, Mike Madagan came to an unavoidable conclusion about his future with the school he has guided throughout its entire university era. He was no longer the right man for the job. Madagan agreed to step down as Lions coach on Monday, following this week's Sunshine State Conference games against Eckerd and Barry. The Lions, 1-13 in conference play and 11-15 overall, already had been eliminated from postseason play by clinching last place in the SSC. He will remain with the school as an adjunct professor and assistant athletic director through the end of the school year. "I have been given every opportunity and resource needed to be successful at Saint Leo both on and off the basketball court," Madagan said in a news release. "While there were many successes and milestones achieved, we lacked the consistency the program I aspired to build needed and deserves moving forward."It is a bittersweet ending for a coach whose values and emphasis on recruiting players of high character never came into question, but who never enjoyed sustained success on the court other than the occasional upset. The Lions had a losing record in each of Madagan's 10 seasons. Though Madagan's Lions produced three SSC Freshmen of the Year and six All-SSC selections, their best finish as a team in the SSC was fourth, in 2004-05. They never made the conference tournament final or advanced to the NCAA II Tournament. Saint Leo's last SSC Tournament win came in 2008. The Lions are 103-183 overall, and 40-112 in SSC games under Madagan. Saint Leo Athletic Director Fran Reidy said Madagan came to him after the school's Christmas holiday break and brought up the possibility of stepping down following the season. Madagan had recently lost his top assistant coach, Ben Thompson, who was offered a job on the support staff of the Virginia Tech men's basketball team. Thompson was never replaced, adding more stress and responsibility to Madagan's job. "He told me the passion and the go was not what it used to be," Reidy said. "He always put Saint Leo University first, the athletic department second, and the men's basketball team after that, which ultimately may have been detrimental to his own personal and his team's success." Reidy has hired an outside consulting firm, Alden and Associates, to conduct a nationwide search for a new coach. Because the current team has seven outgoing seniors, thus plenty of scholarship money to allocate to new recruits, the incoming coach should have ample opportunity to build right away without jeopardizing the scholarships of returning players. "We typically do not take away scholarships from student-athletes," Reidy said. "Traditionally we have not allowed coaches to do that, even though our competitors do that. I think there is enough flexibility for the new coach so that we can keep that policy." Reidy expects to accept applications until March 16 and have a new coach in place by early April. He made it clear what he wants the emphasis to be during the hiring process. "Prior, successful head coaching experience at the college level, first and foremost," Reidy said. Though Madagan's success on the court was never consistent, Reidy believes the values he exhibited could be difficult to duplicate with a new coach. "I think all of our coaches will come to appreciate how much of a team player Mike has been," Reidy said. "He had a lot of wins in other areas. ...Unfortunately, the record only shows wins and losses."
Correspondent Bart O'Connell can be reached at firstname.lastname@example.com.
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Our 2017 Expeditions ARE FULL. However, we do occasionally receive cancellations; if you are interested in being on the waiting list, please call 970.242.0971, ext. 2212.
Bookmark us! Come back later in the year for our 2018 Expeditions schedule!
About Our Paleo Expeditions
- Please note: The various expeditions are for ages 5 and older; each expedition has minimum age noted. Those 16 or younger must be accompanied by a participating adult on all expeditions.
- Gnats are common unwanted visitors in the quarry from May to July and, unfortunately, they do bite. This is one aspect of your experience we cannot control but of which we would like for you to be aware. Participants are encouraged to bring an insect repellent to the quarry with them.
- Weather conditions are unpredictable. Bring hats, sunscreen, sturdy shoes, and sweatshirt or light jacket.
- Although the average high for Grand Junction is 92° in July, it can be 5-10° hotter in the quarry. There are shade tents at the quarry but there is no native shade.
- Online bookings over the weekends and during federal holidays will experience a delay in processing. Please allow enough time for your booking to be processed before the expedition.
- Registration and medical forms must be completed before your registration is complete.
Cancellation / Refund Policy
- Cancellations prior to three weeks in advance of the expedition date will be refunded at 80% of the dig/expedition cost (total cost, less the 20% non-refundable deposit).
- Cancellations within two to three weeks prior to the expedition date will be refunded at 50%.
- All cancellations within one week prior to the expedition date, and all no-shows forfeit full price; no refunds or credits will be issued for unused portions of the Paleontology Expeditions and Tours.
- If the Museum cancels the expedition due to weather or any other reason, we will do our best to make alternate arrangements, reschedule your expedition, or refund your registration in full.
- To make your reservations online, payment in full is required by our Paypal account. We also offer the option of making a 20% non-refundable deposit at the time of registration by calling 970.242.0971, ext 2212. The remaining balance must be paid 15 days prior to the expedition date or a $25.00 service fee will be required to continue holding your reservation.
- These cancellation policies are strictly enforced as our digs and expeditions fill up fast.
For more information, please call 970.242.0971, ext. 2212.
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You can really tell it's wedding season, as Kate was spotted at yet another wedding today! Kate was accompanied by both Princes William and Harry to the occasion, where the Princes' cousin Emily McCorquodale was marrying James Hutt. The wedding took place in Stoke Rochford, a small village located in Lincolnshire. For the wedding, Kate wore a number of recycled pieces, most of which you'll ... View the Post
Kate attends Sam Waley-Cohen & Bella Ballin's wedding with sister Pippa
On June 11th 2011, The Duchess of Cambridge attended the wedding of Sam Waley-Cohen and Bella Ballin. Kate rushed on over to the wedding after attending the Trooping the Colour celebration in the morning. #158146746 / gettyimages.com Kate attended the wedding with her younger sister Pippa. The wedding ceremony was held in Lambourn Church, Berkshire. Kate and Pippa know Sam Cohen ... View the Post


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Entering last year of contract, Wings coach Mike Babcock is unconcerned about signing an extension.
Duff: Babcock intends to stay in Detroit
On the surface, it's much ado about nothing.
It's a story that really isn't much of a story, once you dig a little deeper into the facts and talk about it with the people most involved in the outcome.
Mike Babcock is entering the final year of his contract as coach of the Detroit Red Wings. This is cause for worry among the faithful.
Among the two guys who will be most involved in the decision to get him signed to a new pact – Babcock and Red Wings general manager Ken Holland – there is no worry at all.
"No priority whatsoever," Babcock said of the need for a contract extension. "I'm real comfortable with the owner (Mike Ilitch) and the manager. That's not a concern for me one bit."
Holland, while admitting he hasn't even broached the contract issue with Babcock, was adamant as to who his coach will be next season and for the foreseeable future.
"I want him back behind the bench," Holland said of Babcock. "I respect the job he does for us. I respect his work ethic. I respect the job he did in putting the young players into our system and sticking with them.
"I'm going to talk to him. I thought he did a tremendous job (this season). From the time a team gets eliminated from the playoffs until early August, these are the times for big decisions. I want to talk to Mike here in the next couple of months and we'll see what comes of it."
After he guided the injury-riddled Red Wings to their 23
consecutive appearance in the Stanley Cup playoffs, there was strong support for Babcock as a contender for the Jack Adams Trophy, which goes to the NHL's coach of the year.
During the season, Babcock, who has guided the Wings to a pair of Stanley Cup finals, winning it all in 2007-08, surpassed Adams (413) to become the winningest coach in franchise history.
He also led Canada to its second straight Olympic hockey title at the 2014 Winter Games in Sochi.
Holland understands your unrest. An available Babcock would go to the top of any team's want list.
"I think if you put down a list of the best coaches in the National Hockey League he's on that short list," Holland said. "I think many would have him on that list."
Worry not, though, because the Wings want him to remain right where he is right now.
"I do want him back," Holland said. "We work good together."
Babcock doesn't even care if they decide to talk contract this summer, citing another great coach who worked the Detroit bench prior to him.
"I know Scotty Bowman went year to year sometime here and I'm real comfortable with that," Babcock said. "When you're at the stage of my career that I am, I'm real comfortable with whatever they want.
"I want them to be happy. If they're not happy then I'm not happy. I have no problem. I can go year to year."
In other words, relax – Babcock intends to be coach of the Wings as long as he's wanted.
"For sure, I'm staying in Detroit," Babcock said. "I haven't thought about going anywhere else.
"The owner is better than great to me. They do things right. They treat people right. The GM is a driven guy who can't stand losing and I like to be around ultracompetitive people."
So find something else to fret about. This one is a non-starter.
Babcock is going to be coach of the Wings next season and likely, for many more seasons to come.
His contract might not be long term, but his intentions certainly are.


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SAOIRSE Ronan, who has racked up a dozen feature film credits since being nominated for an Oscar at the tender age of 13 in 2007, didn't set out to work this hard.
But some offers are just too good to refuse. And in the case of this 18-year-old Irish actor, they just kept coming.
When Peter Jackson invited her to collaborate on his adaptation of critically-acclaimed novel, The Lovely Bones, opposite Rachel Weisz and Mark Wahlberg, Ronan could hardly say no.
And what up-and-comer would pass on an opportunity to go head-to-head with Cate Blanchett in a thriller helmed by Joe Wright (who directed Ronan in her Oscar-nominated performance in Atonement).
Then came an invitation from Interview With the Vampire director Neil Jordan to play a key role in his latest discussion with the undead, Bytantium, alongside Gemma Arterton, Jonny Lee Miller and Sam Riley.
And just when it seemed like there might be finally be a chance for Ronan to head back home to County Carlow for some of her "mam's cooking", Wes Anderson's The Grand Budapest Hotel came up.
"I have done three films in a row a few times now, and it can burn you out," Ronan admits.
"It means you can't fully appreciate the project you are doing because you are so knackered.
"I don't really want it to be like that, but sometimes with schedules and stuff, you can't really make that decision."
There are clearly worse problems to have in an industry that averages 90 per cent unemployment - but one feels a certain sympathy for the young actor.
At an age when many of her peers are enjoying a gap year before knuckling down to the pressures of adult life, any mis-step she makes is likely to come under close scrutiny.
And even though the term "child actor" has never sat particularly comfortably with this youngster, the choices she makes now will have a major impact on her adult career.
In spite of the pressures, she says she has no regrets. "You can't really say no to someone like Wes. And I am glad I didn't. I had a great time."
Ronan has plenty to say about The Host, a film adaptation of author Stephenie Meyer's follow-up to the Twilight novels, directed by Andrew Niccol (Gattaca).
The sci-fi romance about a young woman whose body is inhabited by an alien life form, marks something of a departure for Ronan.
But even in the heightened world of teen fiction, she has gone for the dramatic challenge.
"Why not play two women instead of one? That was one of the real draws for me," she says.
The Host takes Twilight's love triangle, between a vampire and a werewolf, and raises it with a love quadrangle.
Since Melanie and Wanda are two separate entities existing in the same body, it makes a certain sense that they ultimately fall in love with two completely different men (played by Jeremy Irons's son Max and Jake Abel).
Ronan said she didn't lose too much sleep over the possibility The Host would introduce her to a whole new celebrity stratosphere.
"I didn't really think about that when I decided to take the project - to be honest, I find it very hard to believe that anything could surpass Twilight for the next little while because it was such a massive phenomenon - but I am definitely aware of it now."
Next up is Ronan's own take on the vampire film - Byzantium, directed by Neil Jordan (The Borgias).
"It was fun to play because it wasn't your typical vampire story - we don't have fangs and we don't turn into crystals in the daylight. It was more of a mythological take on the vampire story, which I really liked."
After a two-month break, Ronan will star opposite Eva Mendes, Christina Hendricks, and Ben Mendelsohn in How to Catch a Monster,, the directorial debut of man-of-the-moment Ryan Gosling.
"He wrote it, too. It's quite dark and very much up his street," she says.
SEE The Host, opens today
Originally published as Former child star in hot demand


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martin sent word that blender is now working on the pocket pc operating system. for those of you not familiar with it, blender is a design and animation tool for creating great -looking 3d images. i read that it's being used to create some 3d games for the playstation 2, but now that it's ported to pocket pc a whole new gaming experience could be coming. check out a video of it in action (it's at the bottom of the page) and read more about it here.
user comments 1 comment(s)
|blender (9:07am est mon apr 09 2001)
cool… – by basko


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Learfield, IMG College Close To Merger Lonzo Ball Talks Shoes, Reality Show Boston Teams Look To Oppose Racism "Conan" Spoofs Fenway Sign Incident Fla. City Voting On Braves' Spring Home Will Scottrade Center Get New Name? Dick's Launching Winter Games Campaign Tiki, Ronde Barber To Call NFL On Fox Fox Leads All CFB Ratings With Texas-USC
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Published November 4, 1994
The U.S. Mint's World Cup commemorative coin program was "a flop" for the government and lost more than $5M -- "the first in modern times to lose money," according to a report in this morning's L.A. TIMES. However, the program netted almost $9M for the World Cup Organizing Committee. Legislation authorized by Congress and signed in 1992 by President Bush, permitted the U.S. Mint to produce over 10M coins of legal tender celebrating the 1994 World Cup, but only 1.5M coins were sold. The Federal government was responsible for designing, manufacturing, marketing, and distributing the coins -- and then paying the World Cup a 25% royalty on any coins sold, essentially guaranteeing a profit. "For example, an uncirculated World Cup commemorative silver dollar sold for $28. Included in the purchase price was the surcharge payment of $7, which went to the World Cup Organizing Committee." Rep. Esteban Torres (D-CA), who sponsored the legislation: "Everyone wants to get into the commemorative coin business for obvious reasons. It's not so much that members want to provide perks. But they are besieged by interest groups -- everyone comes at you." This January, Congress will launch a commemorative coin program for the 1996 Olympics (Julie Cart, LOS ANGELES TIMES, 11/3).


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The Wyatt Earp Drill
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April 17, 2011, 11:01 PM
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Join Date: April 14, 2009
Location: Florida
Posts: 542
I like your tweaks. They can ALL incorporate an unknown element. I have to say maybe I am not explaining correctly? Target distinction is more than just recognizing a threat to you. I HATE HATE HATE to use ABC's dateline or 20/20 investigation, but they DID make a point in their biased study that should be touched upon in drills. They had 2 shooters and 1 that was shooting students actually appeaared to be shooting at the bad guy who came into the room.
I guess basically what I am saying in target distinction is just that you learn to need to be eyes open to everything. Just because it is someone with a gun doesn't make them bad and it also doesn't make them good. I mean don't get me wrong either...al the tactics you are discussing are very important and should be practiced until they are instinct. They can be incorporated into different types of FOF drills though.
Perhaps target distinction can be applied to dogs as well? I will be discussing in another post though lol.
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Getz and Johnson are backed by Oscar Peterson, Ray Brown, Herb Ellis, and Connie Kay – and the tracks are longish, with plenty of solo room in the open jam session mode of the recording. Titles include "Billie's Bounce", "My Funny Valentine", "Crazy Rhythm", and "Blues In The Closet". (Out of print. CRC pressing.) © 1996-2017, Dusty Groove, Inc.
If something is noteworthy, we try to note it in the comments — especially if it is an oddity that is the only wrong thing about the record. This might include, but isn't limited to, warped records, tracks that skip, cover damage or wear as noted above, or strictly cosmetic flaws.
We have only one grade for non-new CDs at Dusty Groove — "Used CD". This grade is somewhat all-encompassing, but we choose it because we try to offer Used CDs in the best shape possible. All of our Used CDs are guaranteed to play without skipping or flaws. If you purchase a Used CD from Dusty Groove, you have 1 week to play it to determine that it plays correctly — and if it does not, then you may return it for a full refund.
With our Used CDs, you can expect the disc to be free of all but the lightest of surface marks — clean, and not dirty at all. You can also expect the case to be clean (we often change the cases ourselves — putting fresh cases on Used CDs we handle) — and you can expect the booklet to be in good shape, unless noted otherwise. We will list any specific details/defects underneath the item — so look for notes on cutout marks in the case, stamps on the barcode, or details like that.


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Tony Stewart's barbed comment about how getting rid of some "dead weight" had helped speed him on his way to a second straight victory in the Chase has set fans puzzling.
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When Tony Stewart was standing in victory lane at New Hampshire Motor Speedway on Sunday, he was asked what had changed to put him back at the top after such a frustrating season up till the Chase cut-off.
"We got rid of some dead weight earlier this week, so it made it a lot easier," he responded to the pit lane reporter on national TV. "It's been a big weight lifted off our shoulders. Just sometimes you have to make adjustments in your life and we did that and it has definitely helped."
So what exactly was Stewart referring to when he said "dead weight"?
His crew chief, Darian Grubb, certainly had no idea. "I've heard a lot of people talk about it, but I haven't heard what he was talking about," he said at the post-race press conference. "I know he went and did a lot of dirt racing and had a lot of fun this week, kind of unloaded everything. Maybe it was a lot of dead weight off his mind."
No one was buying that one. Surely "dead weight" related to a person - had someone been fired from the team?
"Not that I know of," said Grubb. "Maybe he's talking about me and I just don't know it yet!" he added, getting a good laugh from the assembled media.
It seemed that the only person who knew what Smoke was talking about was Smoke, so when Tony Stewart showed up for his own post-race interview it was the first question he was asked by the press.
"We're just going to leave it at that," said Stewart, bluntly.
"Could we ask if it that was ...?"
"You can't ask anything. It is what it is. That's all it was is what you said. That's where we'll leave it," said Stewart, firmly closing the door on any follow-ups.
He did confirm that the comment did not refer to any personnel changes at Stewart/Haas. Darian Grubb was presumably relieved in the sidelines to know that his pink slip wasn't winging its way through the mail to him after all. Stewart's agent was also at the race looking distinctly employed as well, so it wasn't that either.
With Stewart known to be notoriously prickly with the media when riled, no one else pressed on with that line of questioning - but that didn't stop the speculation continuing afterwards both in the press room and with fans online.
The favourite theory is that it related to a break-up with his girlfriend Jessica Zemken, a racing driver in World of Outlaws and Sprint cars, and plenty of consoling messages on her Facebook fan page seemed to make the assumption that the jab had indeed been directed her way.
Stewart would be right to want some privacy for his private life and be annoyed if the media started poking its nose in to matters that didn't concern them. However, the two time NASCAR Cup champion should know better than to use national TV airtime to make barbed comments that he just knows will tweak journalists' interest and leave them scrabbling for more details: if he'd wanted it kept private, he should have kept it private.
It's not as if the NASCAR media entourage isn't unusually respectful of its drivers' private lives - to an extent that raised eyebrows when no one reported on the breakdown of Kurt Busch's five-year marriage to wife Eva earlier this year. That was even though it was common knowledge in the NASCAR paddock, as Eva had been a regular attendee at races until the start of this season when she didn't appear for Busch's Budweiser Shootout and Gatorade Duel victories at Daytona. The media nonetheless decided that it was nothing to do with their coverage of the sport and simply ignored it.
The story only finally broke when Busch was joined in victory lane at Infineon Raceway by a woman later identified as Patricia Driscoll, who hugged and kissed him in the televised celebrations after he won the Sonoma road race. Several reporters felt in the circumstances that they had to explain the context of the images, as it was clear that the woman was not the well-known Eva Busch.
Subsequently the question asked was whether Busch's struggles early in the year were related to the situation with separating from his wife, while his mid-season boost was influenced by a happier personal life.
Now with Stewart's comment about his revitalised Chase form being down to dropping "dead weight" - if it really is related to his private life - it will make it increasingly difficult for reporters to agree that private lives are really none of their business in the future if they're shown to be hard-wired to on-track success.


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How can I remove the stitch marks caused after a thyroid operation?
Stitch Scar Removal After Thyroid Operation
Doctor Answers 3
Scars resulting from stitches can be improved with currently available scar treatments
Scars due to stitches respond to treatments like intralesional steroids, intralesional Bleomycin, Laser and Radiofrequency resurfacing. If the surgery was not too long ago, you could start using creams like Contractubex or Cicatrix to help improve the scar.
Surgical scar revision for neck scars and thyroid scars
Removing stitch marks from a scar is usually difficult without surgical scar revision. In some cases, fractional CO2 laser may work in some rare cases.
Learn more: http://www.surgery90210.com/cosmetic-dermatology/38/scar-revision.aspx
Yes. The scar can be revised
The answer to your question is that a variety of scar camouflage techniques are available. However, it is important to note that scars will improve naturally over the course of a year. Unless there are serious aesthetic issues with a scar that are unlikely to resolve, I encourage patients to wait for this time frame. If the scar is still objectionable at 1 year (wide or hypertrophic), I would recommend surgical scar removal and revision. If the scar is raised or firm, a trial of steroid (kenalog) injections might be more appropriate.
You might also like...
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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Salvation Army offers help on energy bills
Posted December 1, 2014
Fayetteville, N.C. — Cumberland County residents stood in line Monday at the Salvation Army Social Services building, waiting their turn to apply for help paying their energy bills.
"Because my light bill sometimes is so high, I always have to ask for help to pay it," said Fayetteville resident Alene Johnson, one of those in line.
The Salvation Army has about $2 million to provide energy assistance to seniors and those with special needs. Recipients don't get a check to pay their bills. Instead, the money goes directly to the utility.
When the organization opened the application process in February, the crowd wrapped around the building before the money ran out. The crowd was smaller Monday, partly because Salvation Army staffers conducted outreach at senior living facilities, but the desperate need for help was the same.
"I go to school full time at Fayetteville Tech, so I don't really have enough money to pay the deposit because I just moved into a new home," Kimberly Diaz said. "The deposit is like $350, and the heat deposit is like $150."
The Salvation Army has beefed up its staff since the last application process, but things still slowed at times. Applicants need to bring proper identification.
"We just want folks to realize that, to be patient with us and to make sure that they have everything that they need when they get here so that they can be seen as quickly as possible," said Jason Smith, director of the Salvation Army branch that serves Cumberland, Scotland, Moore, Robeson and Hoke counties.
The program runs until March 31 or until the money runs out.
"This is just a blessing for me and a blessing that I'm just thankful for it," Johnson said.


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BBC Review
...arresting, intimate and evocative in equal measure.
Charlotte Gardner 2008
Between the ages of 19 and 37, Giacomo Rossini wrote 40 operas. He then turned his back on the genre for the rest of his life. Thankfully for us, he didn't spurn vocal music per se; in addition to his Stabat Mater and Petite Messe Solennelle, he composed three collections of songs, Soirées Musicales, Péchés De Vieillesse and La Regatta Veneziana, many of which were performed at the Parisian salon evenings he hosted with his wife. This selection of them for Hyperion is arresting, intimate and evocative in equal measure.
Soirées Musicales are the first set and, whilst Rossini may have been sick to the back teeth of the stage, these elegant songs capitalise on his dramatic talent, the writing at times sounding more than a little reminiscent of his operatic work. Péchés De Vieillesse or Sins Of Old Age were composed 20 years later, by which time Rossini's salons were famous, attended by the likes of Boito, Liszt and Verdi. Despite the jollity of the evenings, the songs themselves are undeniably the work of an older man, with the gloomy topics of death and exile pushing in alongside the declarations of love.
There isn't a low point on the entire disc. Roger Vignoles plays with all his characteristic intelligence and subtlety. Miah Persson is enchanting in the opening La Promessa, and playfully dazzling in La Pastorella Dell'Alpi. Stella Doufexis' pure, rich voice gives perfect expression to L'orpheline Du Tyrol, and the two women's voices meld together magically when paired in duets such as La Pesca. Meanwhile, Bruce Ford is technically brilliant and dramatically engaging in the drinking song, L'orgia, and smoothly leads the mood from light to darkness with L'esule, the first of the Péchés De Vieillesse.
If Rossini's songs are the cake and the performers the icing, the recording itself is the unexpected cherry on top. The acoustic of Potton Hall in Suffolk immediately transports you to the intimate space of Rossini's salon, candles flickering, guests listening intently, adding a further dimension to this already wonderful disc.