text:
GREENSBORO, NC -- This is fact or fiction for college graduates as they enter the workforce. Certified Financial Planner Matt Logan of Matt Logan, Inc. put together this list:
Fact or fiction: You will be at your first job for the rest of your life.
Fiction: Many graduates put so much stress and emphasis on their first job, expecting it to be their end all be all. This is simply not the case. A study commissioned by Devry University's Career Advisory Board and conducted by Harris Interactive showed that Millennials will more likely have 12 to 15 jobs during their working years. That being said, look for a first job that may be a good stepping stone to your eventual career goal. Gain experiences and skills to prepare you for your ultimate dream job. Treat your early working years as resume building years and do not stress if you do not find your dream job or land in a field that is not your dream field right out of school.
Fact or Fiction: I should put off retirement savings until later in my career.
Fiction: Whether your first job out of college has a retirement plan or you have to start one on your own, you should begin saving as early as possible. If you make it part of your routine, it will help you for years to come. A Fidelity study compared the difference between investing $5000 a year in your IRA at the beginning of every year with a hypothetical return of 7%. If you were to start at age 25 you would have $1,641,122 at the age of 70. If you waited 10 years and started at age 35, you would have $796,687 at age 70. Lesson here, start young and invest regularly. Use the power of compounding early. That money will last a lot longer than the new car, new clothes or expensive vacation that you think you need.
Fact or Fiction: I need to have health insurance.
Fact: You are now officially an adult and on your own. It is no longer your parents responsibility, you need to make sure that you have health insurance coverage on yourself. I know you may not have been to the doctor in years and are the epitome of health. I get that. Nobody plans on needing medical attention in their 20s. You need to be sure you are prepared because a medical catastrophe can harness you with insurmountable debt.
Fact or Fiction: I need to buy a house.
Fiction: You are just out of school. Enjoy it. Home ownership comes with a lot of responsibility and is also something that will tie you down. One of your competitive advantages in the work world is your flexibility and that includes location. Most probably you do not have a family of your own yet. Put that off a bit until you have furthered your career. While owning a home is important, do not rush into it.
Fact or Fiction: I deserve to be in management.
Fiction: You deserve nothing. Employers main gripe with the Millennial Generation is the sense of entitlement. Prove them wrong. Tell prospective employers you want to earn a place in their company. Employers are saying that Millennials expect to go straight from college to the executive suite. That simply does not happen. Your career is more like a marathon than a sprint.
Fact or Fiction: Moving back in with mom and dad is a smart financial decision.
Fact: While it may not sound sexy, moving in with mom and dad after college can be a very wise decision. It may sound way more appealing to risk it all and move to New York City and try to live on credit cards and ramen noodles until you land your dream job, but moving home with the parents is not always a bad thing. Even if your parents require you to pay a nominal rent, moving home to pay off college debt and begin your working career is not always a bad thing. Chances are you have some connections in your hometown that could give you a leg up on landing your first job. I know you parents out there may cringe to hear that. Your job is to make sure you do not make life to comfortable at home, so when they are on their feet both job wise and financially, be sure to edge them out of the nest.
Fact or Fiction: Education debt is always good debt.
Fiction: The old saying is to always invest in your education. This is most of the time true but not always. Be sure that you know what you are getting into ahead of time when it comes to your education debt load. If you are going back to school for a graduate degree or further higher education, you should look hard at the information on graduates of that program. How many land careers in their field and what is the average pay? While it is rare, you can set yourself up for financial disaster by furthering your education into a career that cannot pay for your education debt.
You can also go to my website www.mattloganinc.com for more information on financial topics you may find helpful.
Matt Logan is a Representative with Matt Logan Inc and Summit Brokerage and may be reached at www.mattloganinc.com, 336-808-0126 or firstname.lastname@example.com.
Matt Logan Inc is an independent firm with Securities offered through Summit Brokerage Services, Inc., Member FIRNA, SIPC. Advisory services offered through Summit Financial Group Inc., a Registered Investment Advisor.


text:
Research Topics
R C Woodman
Affiliation: University of Calgary
Country: Canada
Detail Information
- The functional paradox of CD43 in leukocyte recruitment: a study using CD43-deficient miceR C Woodman
Immunology Research Group, University of Calgary, Calgary, Alberta, Canada T2N 4N1
J Exp Med 188:2181-6. 1998..These results suggest that leukocyte CD43 has a dual function in leukocyte-endothelial interactions. In addition to its role as a passive nonspecific functional barrier, CD43 also facilitates emigration of leukocytes into tissues...
- Thrombin and leukocyte recruitment in endotoxemiaR C Woodman
Department of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1
Am J Physiol Heart Circ Physiol 279:H1338-45. 2000..If AT III has beneficial effects in endotoxemia, it is not due to a direct effect upon leukocyte rolling, adhesion, or emigration in postcapillary venules in vivo...
- Chronic inflammation upregulates chemokine receptors and induces neutrophil migration to monocyte chemoattractant protein-1B Johnston
Immunology Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta T2N 4N1, Canada
J Clin Invest 103:1269-76. 1999..It also demonstrates that CD18 is required for chemokine-induced leukocyte transendothelial migration, independent of its known role in mediating firm adhesion. J. Clin. Invest. 103:1269-1276 (1999)...
- Selective recruitment of neutrophils and lymphocytes by thrombin: a role for NF-kappaBJ Kaur
Immunology Research Group and Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada T2N 4N1
Am J Physiol Heart Circ Physiol 281:H784-95. 2001..Although NF-kappaB mobilization was essential for thrombin-mediated VCAM-1-dependent recruitment, it only partially contributed to E-selectin-dependent recruitment...

subset: commoncrawl


text:
Is there one link, story, picture or thought that you saw online this week that you think somebody you know must see?
My friends: Alistair Croll (BitCurrent, Year One Labs, GigaOM, Human 2.0, the author of Complete Web Monitoring and Managing Bandwidth: Deploying QOS in Enterprise Networks), Hugh McGuire (The Book Oven, LibriVox, iambik, PressBooks, Media Hacks) and I decided that every week or so the three of us are going to share one link for one another (for a total of six links) that each individual feels the other person "must see".
Check out these six links that we're recommending to one another:
- How a collapsing scientific hypothesis led to a lawsuit and arrest - Ars Technica. "Science and hubris are strange bedfellows. I loved this story, which reads like a nerdy Grisham novel, because it gives you a glimpse into what it's like inside the pressure cooker of publishing and research. It's also a strong argument for peer review and transparency in research." (Alistair for Hugh).
- Passive Aggressive Notes. "Something lighter for the holidays. My grandfather seldom asked for tea, instead, he'd ask, 'is the kettle broken?' He'd have identified with the authors of this collection of passive-aggressive notes, signs and suggestions. Something to keep in mind when you open a present this year and say, 'were all the regular stores closed?'" (Alistair for Mitch).
- How Iceland survived the fire - Financial Times. "I am obsessed with our response to the financial crisis that started in 2008 and has no end in sight. It's refreshing to see what Iceland did: banks who made stupid loans were told to suck it up and suffer the consequences. The Iceland government didn't backstop the shitty financial decisions of big finance companies. The world was shocked! Appalled! Iceland would suffer. Except they haven't. Iceland has done shockingly well, compared to Ireland, which took the opposite path: guaranteeing all manner of shitty financial decisions by their own banks and foreign banks, subjecting the country to years of woe. Of course, Iceland and Ireland are small economies, and small countries, but as laboratories for different kinds of responses to this crisis, they are instructive." (Hugh for Alistair).
- You Say You Want a Devolution? - Vanity Fair. "This is something I've been thinking about for a while now, wondering about the reasons behind it: Today is 2011. Imagine 20 years ago, 1991: what was on the radio? How did people dress? Is there that much difference? Picture the same thing, between 1991 and the sideburns and bellbottoms of 1971. And then the sideburns and bellbottoms of 1971 versus the behives and swing jazz of 1951. It seems certain parts of our culture have stopped changing very much: music and fashion. What's going on? I don't quite agree with all of the author's conclusions, but it's fascinating, nonetheless." (Hugh for Mitch).
- Can You Learn To Code In One Day? We Sent A Non-Nerd To Find Out - Fast Company. "I've been on a kick lately and it involves me (and my kids) learning another language. The language of code. I'm both fascinated with the idea of taking a stab at it and terrified because I don't know where (or even how) to begin. Moving forward, I do think that 'coders are the new creative,' as Faris Yakob says. When I think about history, I think about all of the architects and designers it took to build our physical society, well the evolution of our society is, clearly, a digital one, so the coders, programmers and architects of our future need to know this one, very important, language... the language of code." (Mitch for Alistair).
- Most print newspapers in the States have only a five-year life span - The Guardian. "This isn't the ramblings of a lone Blogger hell-bent on building their own new media empire. This statement comes a soon-to-be-released report titled, Is America at a digital turning point?, by the University of Southern California's Annenberg centre for the digital future. According to the report: 'Circulation of print newspapers continues to plummet, and we believe that the only print newspapers that will survive will be at the extremes of the medium - the largest and the smallest.' That - in and of itself - forces us to stop asking questions about when newspapers will go away and start doing the very hard work of trying to figure out what comes next and what we're going to do about?" (Mitch for Hugh).
Now it's your turn: in the comment section below pick one thing that you saw this week that inspired you and share it.


text:
Advice for grads from Yale j-director, ESPN's Reilly
Good Men Project Magazine | Westword
"Have humble expectations," says Yale Journalism Initiative director Mark Oppenheimer. "Your first job will pay you too little, I promise." He points out that for a job interview, flip-flops aren't acceptable and "piercings that look painful enough to make people wince may cost you jobs. Employers do not want their first impression of you to be what an individual you are."
At the University of Colorado, ESPN's Rick Reilly was picked to speak to the journalism school's last graduating class. He said to them:
So, you guys are the last graduating class from the CU J-school. And I'm the last Distinguished Alumnus guy from this place. I feel like we're christening the Hindenburg here. It's like, 'Hey, congratulations, you made the last really good buggy whip! Turn off the lights when you leave, OK?'
When you get out there, all I ask is that you: DON'T WRITE FOR FREE! Nobody asks strippers to strip for free, doctors to doctor for free or professors to profess for free. Have some pride! What you know how to do now is a skill that 99.9 percent of the people don't have. If you do it for free, they won't respect you in the morning. Or the next day. Or the day after that. You sink everybody's boat in the harbor, not just yours. So just DON'T!


text:
Who is your Everyday Hero?
Our four weeks of Everyday Hero nominations and awards are over, and we learned about some remarkable people. You can read about them, below.
Special thanks to everyone who was nominated - each of you are an Everyday Hero! And thanks to all those who took the time to nominate; it is great that these folks are getting recognition for their efforts.
We will keep the information about the nominations here on this page so that anyone who visits gets a better idea about what we were trying to do.
Nominate an Everyday Hero and they could win $250
Do you know anyone who has committed a random act of kindness?
Do you know anyone who is paying it forward?
"Everyday Heroes" are all around us. They are individuals and organizations who freely donate their experience, talent, time and hard work in an effort to benefit folks who aren't as fortunate. What do they have in common? They all have the ability to make a personal connection that frequently turns into a pay-it-forward wave that spreads around their local community and our world.
Each week, nominate a person or an organization that has inspired you with their acts of selflessness in our community.
Submit the name of your Everyday Hero here on our Facebook page, along with your reasons for nominating them.
Drawings will be held every Friday and you will be notified via Facebook if your nominee was chosen to win $250.
We will pick one winner every week for four consecutive weeks.
Employees and families of affiliated companies are not eligible to win. No purchase necessary. If a winner is unreachable after seven (7) days, or if that winner is unavailable for prize fulfillment, an alternate winner will be selected. Winner will assume any federal or state tax liability that may be incurred. Winners will be randomly selected. Promotion dates: 2-11, 2-18, 2-25, 3-3
By entering this contest you are adding your address to Airport's database.
Our Week 1 Winner was Jennifer Hedgepeth. Her friend Suzi Mcclendon gave us this nomination:
"I think that Jennifer Hedgepeth is a hero. This lady wears many hats. She helps anyone in need. She is always volunteering her time, helping with many different organizations. She is wonderful!!!"
Thank you for all you do, Jennifer!
Our Week Two Everyday Hero is JoLayne Fulmer.
Here is what her friends had to say:
Annie Laurie King: "My everyday Hero is JoLayne Fulmer. She lost her daughter, Faith, to cancer just 5 1/2 months ago. She has started Faith Fulmer Foundation and is helping Dr. Keller at OHSU find a cure for childhood cancer. 46 kids a day are diagnosed with cancer and 7 die a day. The money would help this research so other parents don't lose their child."
Heidi Hoggatt Loftus: "JoLayne Fulmer continues to collect, package up and send toys to other children and families faced with childhood cancer. She recently helped a local family who requested a bed to sleep next to their child who is dealing with cancer. JoLayne immediately put the request out to her network of friends and family who responded instantly and that evening the family had what they needed and more! She doesn't hesitate; she jumps at the chance to help and follows through. She does this with love and compassion more often than we know."
Thank you JoLayne for everything that you do; you are an inspiration to us all!
Our Week Three Everyday Hero is Doctor RD Jones, and here are two of his nominations:
Rach Rogers: "An everyday Hero? This man has distinguished ability to care for our children. He has the ability to put the needs of his patients far above his own. He is always just a phone call away. He takes the time to seek the answers to questions when everyone else tells us there is no hope. He is a man of integrity and shows this by his actions.
This man is Doctor RD Jones Of The Kids Clinic.
The level of commitment that the Kids Clinic staff has is immeasurable. The love and dedication that this office has is incredible. The Rouge Valley is blessed beyond measure by having this team."
Emilie Sampson: "I second (or 4th) the nomination of Dr. RD Jones. He has gone above and beyond in his care for my boys. He, along with all of the staff at the Kids Clinic have been a huge support for families who have children with disabilities. He has kept the clinic open after hours to ensure that we would not have to head to Urgent Care or the ER."
Thank you Doctor Jones and staff at the Kids Clinic for your efforts in our community. You obviously have an inspired following!
Our Week Four Everyday Hero is Chloe Christiansen, and here is one of her nominations:
Heath Christiansen: "I nominate my teenage daughter Chloe Christiansen as an everyday hero! She goes to school and works and still volunteers her time whenever she can. She volunteers at the hospital, redcross, relay for life, at local events, and she raises money for individuals who need it most. She always will help anyone who needs it! She is a local hero to many including myself!"
Chloe, you are awesome! Keep up the good work and keep setting a good example for all of us to follow.


text:
Seibertron.com - The Ultimate Transformers Fansite
rpetras wrote:If they scaled him down so he would fit better with G1 figures, they may find a market for this.
He'd look right in a G1 collection.
Cyber Bishop wrote:Hopefully this one will be easier on the wallet than the Igear one and I will be able to afford him.
warzon3 wrote:If I recall correctly, you pay up front for Robot Kingdom preorders?
warzon3 wrote:I think on their facebook that they mentioned they wouldn't have final pics until early November? I hope they really can still pull off October or at least early November.
I wanted to see pics first, but I caved and ordered not too long ago.
I feel like I'm the only one who is interested in them, I hope nothing happens where they pull out do to lack of interest. I'm sure everyone got the Igears versions over these guys....seems like bad timing.
Return to Unlicensed and KO Transformers Toys
Registered users: Autobot Roadburn, Bing [Bot], bodrock, BombshellDaBug, Exabot [Bot], Google [Bot], Google Adsense [Bot], Kurona, Majestic-12 [Bot], Nexus Knight, Quantum Surge, Qwan, Rampage01, Sigma Magnus, skywarp-2, sto_vo_kor_2000, Tigertrack, Tyrannacon, Yahoo [Bot]


text: so i just grabbed the mesmerize at my local USCC store and i was planning on rooting it like my other android phones that i have had..Ive been reading alot about how to root it and im still kinda having trouble. the software version is 2.3.5 E120, and most people when they talk about rooting this phone seem to have 2.3.4 EH09 or something like that... am i able to root my phone with that software? if someone can just kinda push me in the right direction that would be great. I know that it involves using a program odin and flashing stuff onto it but im very skeptical and confused about how to do that. Ive been reading threads on almost every forum including rootzwiki so if someone can just give me a tiny bit of help im sure i can get this all figured out. thanks!


text:
With all his policy proposals effectively snubbed by Prime Minister Taro Aso, Liberal Democratic Party lawmaker Yoshimi Watanabe's exit from the ruling party became almost certain Friday.
Watanabe said that in the morning he tried to hand an open letter to Aso that questioned him about civil servant reform. But Aso's secretary refused to accept it and instead told Watanabe to raise the question in the Diet.
Asked what he plans to do next, the former administrative reform minister said the matter of his exit "has come to a crucial point."
"Abolishing 'amakudari' and promoting civil servant reforms are the themes that I have been working on with all my effort and determination," he said, referring to the practice of retiring top bureaucrats landing lucrative, and often allegedly corrupt, jobs in industries they once oversaw. "If (Aso's policy) is fundamentally different or goes backward, it can be a cue for me" to leave the LDP, he said.
On Monday, Watanabe disclosed his seven policy proposals and said he would leave the LDP unless the proposals were not seriously and promptly considered.
Watanabe's likely exit from the LDP could cause a stir among the party's ranks that support his position. His opponents within the LDP have labeled his move a staged play to draw Aso's refusal and justify his exit.
Watanabe's proposals made Monday include calling an early general election, canceling the ¥2 trillion cash handout program and promoting civil servant reform.
Having observed Aso's response Thursday at the Lower House Budget Committee, Watanabe said he wanted to confirm two things with Aso.
One is whether Aso will completely ban the "watari" practice in which ministries can arrange new jobs multiple times at government-related corporations for retiring bureaucrats, which is often criticized as a waste of taxpayer money. In many cases, the ex-bureaucrats get vast retirement packages each time they change jobs.
The other is whether Aso plans to scrap a government ordinance that grants the prime minister authority to approve bureaucrats' job outplacement and also allows multiple job outplacements on rare exceptions.


text: Used to be in my district that tenured had one scheduled observation per year, non tenured had 3-4. Our state is now requiring new eval systems. Some have signed on to Marzano, Danielson, etc. we are doing Marshall...ten ten-minute observations, drop in, per teacher. I've been observed twice already...so ar, so good...gotta say, I do think unannounced minis paint a more realistic 'snapshot' of a classroom and teacher practices than a once a year dog and pony show...that plus my P does a non-observation swing thru most as anyway so it's comfortable, I feel like he understands who I am as a teacher and that my observations won't be 'gotcha' situations. 8 more to go... Any changes in observations/eval for you?


text:
The 20-second spot, for Trident Splash, was created by JWT's Phillip Meyler and Darren Keff and constructs a metaphor for the experience of chewing Trident gum.
It features a gum-chewing young woman in an airport queue, whose mind takes her to an imaginary world where she experiences a surreal adventure with synchronised swimmers, a performing sea lion and a spacehopper ride through a fifties B-movie set.
She jolts back to reality as her spacehopper explodes, squirting green liquid all over the screen. It closes with the campaign endline: "Mess with your head".
The ad breaks tonight and is the first of two ads directed by Traktor through production company Partizan.
It was shot in and around LA by Traktor's crew, which included Bill Pope, director of photography for 'The Matrix' and 'Spiderman' films.
Russell Ramsey, executive creative director of JWT, said: "They're cool entertaining films that make their point in a crazy way."
The "Mess with your head" campaign is the second for Trident since it launched last year with the controversial "mastication for the nation" campaign.
The ads aired across TV and cinema featuring black and white people speaking with strong Caribbean accents.
More than 500 viewers complained to the ASA that the ads showed offensive stereotypes and ridiculed black or Caribbean people. The campaign was subsequently banned for being racist and offensive.


text: Hi, I am trying to install sheepdog on latest proxmox VE 2 . And i am getting errors while compiling sheepdog as follows configure: Sanitizing prefix: /usr configure: Sanitizing exec_prefix: NONE ./configure: line 6490: syntax error near unexpected token `corosync,corosync' ./configure: line 6490: ` PKG_CHECK_MODULES(corosync,corosync)' is it possible to include sheepdog with proxmox 2? Thanks, Joby Xavier


text:
Northern Rail will continue to operate regional rail services across the north of England until February 2016.
A decision on the franchise, which includes services on the Airedale, Caldervale and Wharfedale lines, was reached yesterday after negotiations with the Department for Transport (DfT).
Northern has committed to a range of improvements as part of the agreement, including improving ticket purchase facilities, extending the availability of advanced tickets, installing customer information display screens at a further 100 stations, and funding environmental initiatives.
Alex Hynes, Northern Rail managing director, said the deal was reached following the signing of the new Direct Award agreement with shareholders Serco and Abellio.
"We're already delivering enhancements across our network, our services are more punctual and reliable, and the commitment is there to drive further efficiency with our partners," he said.
"Crucially, today's announcement gives us the opportunity to start consulting with all our stakeholders on the requirements for creating a step change in rail services for the North under the next long term franchise."
Northern will continue to work on proposals to deliver a fleet of electric trains to replace diesel stock.


text:
Why Buy Chrysler?
Chrysler would be the first major automaker to become a private company. The big question is what will Cerberus do with the ailing car maker? Colin Blaydon, professor at Dartmouth's Tuck School of Business, talks about the deal.
STEVE INSKEEP, host:
Cerberus, the private equity firm that's buying Chrysler, has been trying to make an inroad into the auto industry for years. A company which buys troubled corporate castoffs now appears to be assembling one of the world's biggest automotive companies. It's put together a group of auto-related assets, including General Motors's former financing unit and the company that owns the Alamo and National Car Rental chains. Which raises the question whether Cerberus can save Chrysler.
We put that question to Colin Blaydon, who teaches at the Tuck School of Business at Dartmouth.
Professor COLIN BLAYDON (Dartmouth): What they are good at doing is really transforming companies. That's what they're going to be looking to do here. But it's very different from the big private equity deals we've been seeing recently.
INSKEEP: How so?
Prof. BLAYDON: Well, the reason we've gotten to know these firms, and they've been in the news so much lately, is because they have been able to raise so much money and buy very big companies. There is not a lot of money being put on the table for Chrysler.
INSKEEP: What can a private equity firm do with Chrysler or perhaps to Chrysler that Daimler was unable to do with them?
Prof. BLAYDON: One thing we have been seeing in the last few years is that the private equity model seems to be very effective. And by that I mean take a company, have a very focused board of directors that can make tough decisions and pay close attention because it's their investment money in it, and then put in place an incentive structure for the management team and the workers that really get them focused and have a big stake in the transformation of the company. That's something the big auto companies have not done.
INSKEEP: Can I just ask one more question? In and among all these efforts to cut costs and restructure the company and prepare possibly for going public again at some point after things have been restructured, do you think that the new executive team is going to have time to think about, well, making and selling better cars?
Prof. BLAYDON: They're going to have to. Because if they do not create value that's recognized in the marketplace, they're going to fail. There is a broad misunderstanding about these private equity firms that, you know, they strip assets and steal away in the middle of the night. They can't make money by doing that, and particularly they can't make money doing that with a company like Chrysler.
INSKEEP: We've been listening to Colin Blaydon. He teaches at the Tuck School of Business at Dartmouth. Thanks very much.
Prof. BLAYDON: Thank you.
Copyright © 2007 NPR. All rights reserved. Visit our website terms of use and permissions pages at www.npr.org for further information.
NPR transcripts are created on a rush deadline by Verb8tm, Inc., an NPR contractor, and produced using a proprietary transcription process developed with NPR. This text may not be in its final form and may be updated or revised in the future. Accuracy and availability may vary. The authoritative record of NPR's programming is the audio record.
Q&A: Under the Hood of the Chrysler Deal
Germany-based DaimlerChrysler will soon lose the second half of its name. The company announced Monday that it is selling 80 percent of the Chrysler Group to a private equity fund for $7.4 billion. The new owner, Cerberus Capital Management, inherits an automobile company that is losing money and laying off workers. The deal essentially reverses a much-touted 1998 merger of the two automakers. Here, a look under the hood at the sale:
What is Cerberus? I've never heard of it.
It is a private-equity firm — named after the three-headed dog that guarded the gates of hell in Greek mythology. The firm, founded in 1992, currently owns about 50 companies, ranging from Air Canada to Formica Corp. Of late, it has taken an interest in the auto industry. It owns the car-rental firms National and Alamo and has a controlling interest in General Motors' financing arm. Former Treasury Secretary John Snow is Cerberus' chairman.
Is this the biggest takeover by a private-equity firm?
No, but it is certainly one of the most high profile. For the first time, a U.S. automaker — and American icon — is in the hands of a private-equity firm, not accountable to shareholders.
What happened to the much-heralded merger between Daimler-Benz and Chrysler a decade ago?
It never stuck. At the time, it was called a "merger of equals" and a "marriage made in heaven." As it turns out, it was a less-than-ideal union —- with Chrysler's profit line after the merger resembling the path of a roller-coaster. Dieter Zetsche, the chief executive of DaimlerChrysler, admitted as much at a news conference Monday in Germany. "We obviously overestimated the potential of synergies," he said.
Critics of the merger say it failed, in part, because Daimler was reluctant to share its high-end technology with the more down-market Chrysler products. "Daimler didn't want their Mercedes owners looking under the hood of their car and seeing the same thing under the hood of a Dodge," says David Healy, an analyst with Burnham Securities.
Under pressure from angry shareholders, Daimler was eager to unload Chrysler — even at a fire-sale price — and rid itself of what had become a financial albatross. The way the deal is structured, Daimler is actually paying to unload Chrysler.
Will Daimler play any role in Chrysler's future?
Yes. Daimler will retain a nearly 20 percent share in the new Chrysler, and some joint research projects will continue.
How poorly is Chrysler doing as a company?
Very poorly. It posted an operating loss of $1.5 billion last year, closed one plant and recently announced 13,000 layoffs. It is saddled with high labor costs, dwindling sales and an excessive number of dealerships. And Chrysler, like other U.S. automakers, has been slow to introduce fuel-efficient hybrid cars, like the popular Toyota Prius.
What does the deal mean for Chrysler's 80,000 employees?
That's not clear. Traditionally, private-equity firms that buy a company like Chrysler reduce costs drastically — often through layoffs — then sell the company at a profit. That's why Chrysler President Tom LaSorda was quick to reassure employees by saying that Cerberus has "a long-term commitment to Chrysler's growth and success." Cerberus Chairman John Snow argues that a private firm, free of shareholders hunger for quarterly profits, can actually take a more long-term view. "We don't think about the next quarter. We don't think about what analysts have to say about us," he said. Many analysts, though, say future layoffs at Chrysler are inevitable, given the amount of cash the company is hemorrhaging.
How do the labor unions feel about the sale?
They support it, which is surprising because just a few weeks ago, the head of the biggest union, the United Auto Workers, publicly opposed the sale of Chrysler to a private-equity firm. UAW chief Ron Gettelfinger said he feared such a firm would "strip and flip" the company to turn a quick profit. It's not clear why he had a change of heart. The deal, though, may represent the best of several bad options for the company — and the union. On Monday, Gettlefinger said the union had received some "commitments" from Cerberus, but he didn't specify what they were. In any event, the tough decisions won't be made until this summer, when management and the union begin negotiating a new four-year contract.
Who absorbs Chrysler's health care and pension liabilities?
Cerberus does. And they are costly: around $18 billion or more, according to some estimates.
What does the deal mean for Chrysler consumers?
Not much, at least in the short term. Warranties, for instance, are protected under law, even if the company is sold. In the medium term, the new owners may launch some innovative new models in hopes of winning back market share.


text:
In celebration of Arizona's centennial, the Star will feature our picks for the 100 best athletes, moments and teams.
Throughout the summer, we will showcase our list - with the first 90 in no particular order. In August, Greg Hansen will choose his top 10, with a column on each.
Vance Johnson
Johnson, a Cholla High School graduate, won an NCAA championship in the long jump and went on to play on three Super Bowl teams. As an Arizona Wildcats freshman, he started four games at tailback in 1981. The following season, he earned All-Pac-10 first-team honors.
The Denver Broncos drafted Johnson in the second round in 1985. He played in Super Bowls in 1987, 1988 and 1990 as a starting receiver with Denver.
In Super Bowl XXI, he caught a 47-yard touchdown pass from John Elway and finished with 121 yards receiving in the 39-20 loss to the New York Giants.
In his best season, Johnson finished with 1,095 yards receiving with seven touchdowns in 1989. Along with Mark Jackson and Ricky Nattiel, Johnson was one of Elway's targets known as the "Three Amigos."
Away from the field Johnson has faced legal trouble, including brief jail time and incidents of domestic abuse. He wrote a biography, "The Vance: The Beginning & The End" about his troubled past.
After his retirement, Johnson was involved in trucking and mortgage businesses, according to the Denver Post.
Johnson is an uncle of former CDO H.S. standout Ka'Deem Carey, who will play at the UA.
Hometown; current age
Trenton, N.J.; 48
By the numbers
Career receptions (5th all-time for Denver)
Career receiving yards (seventh)
Career TD receptions (seventh)
Sarah Trotto


text:
News BulletsBy Caillan
June 5, 2001 - 1:15 PM
- The official site reported that Michael Dorn (Worf) will be moderating a panel of behind-the-scenes Trek luminaries at "A Festival Of Visual Effects" to be held on June 22-24 at the Los Angeles Film School. Further details can be found here.
- Digital Views.com has posted a review of 'Star Trek: The Motion Picture.
- StarTrek.com has conducted another Sensor Sweep of Trek alums on film and television.
- Zap2it.com has posted a round-up of all the science fiction shows debuting and returning this fall, including Enterprise.
- Rick Norwood's latest Babylon 5.1 column at the SF Site includes an episode guide to Voyager's seventh season. "This was, by a wide margin, Star Trek: Voyager's finest season," Norwood wrote.
- Totally Kate! has added further photos to its "Women of Star Trek: Voyager" convention page.
- Edward McArdle has posted a short report on the recent Holodiction Odyssey convention held in Melbourne, Australia. The report mostly consists of photos of the guests, Garrett Wang (Harry Kim), Tim Russ (Tuvok), Roxann Dawson (B'Elanna Torres), Robert Picardo (The Holodoc), Robert O'Reilly (Gowron) and J.G. Hertzler (Martok).
Discuss this news item at Trek BBS!
Add TrekToday RSS feed to your news reader or My Yahoo!
Also a CSI: Crime Scene Investigation fan? Then visit CSIFiles.com!
Find more episode info in the Episode Guide.


text:
Bringing the feeling of summer to shoppers throughout the winter, Santa Sweets Inc. plans to extend demand for its UglyRipe tomatoes through a new marketing campaign.
Signifying a move toward bigger marketing campaigns for the Plant City, Fla.-based Santa Sweets, the "Taste Like Summer" campaign plans to encourage shoppers to place the trademarked heirloom tomatoes in their shopping baskets.
Santa Sweets has increased acreage and is targeting Mid-Atlantic and Northeastern markets December through March to remind them that summertime local tomato flavor is available throughout the year, said Rick Feighery, vice president of sales for Santa Sweets and Philadelphia-based Procacci Bros. Sales Corp.
"Every time I read an industry publication or foodie magazines, it's all about flavor, flavor, flavor," he said. "We have always done well with our UglyRipes in Florida. We're trying to keep the momentum we have going into Florida into the Northeastern states for when all the snowbirds go home. They look for them in the stores."
The campaign features summer-themed point-of-sale materials including in-store displays, bins and placards and Santa Sweets plans to promote the tomatoes through social media and use a consumer website, http://tinyurl.com/SantaSweets.
Feighery declined to state acreage.
Santa Sweets grows tomatoes in north, central and south Florida, North Carolina and New Jersey.
Santa Sweets is owned by Joseph Procacci, Procacci Bros.' chief executive officer and chairman of the board.


text:
Category: Paris baby style
I was lucky enough to go to Paris recently, and like most tourists, I ate croissants in sidewalk cafes, visited museums, and walked along the Seine. Unlike most tourists, I also investigated the chicest French baby names.
"Prune," pronounced a very stylish and knowledgeable Parisian woman of my acquaintance. "Prune is the newest, most charming name for little girls."
It's hard to imagine Prune as a charming name for a child in any country, until you realize that in French it's the equivalent of Plum. Now THAT makes sense.
For boys, this same friend offered the name Illan, another unlikely translation to English. In France, it's pronounced Ee-lahn and sounds quite elegant.
Another friend, who volunteers at a Montessori preschool near the Louvre, said her class includes children named Capucine, the French for nasturtium; Frostine, best known to American children as the queen in Candyland; and Zingo — though Zingo (a boy) is Japanese.
Further investigation turned up the following French baby names on the Paris most-chic list:
ADELE — Asleep in the U.S. but sprightly in Paris.
ANNAELLE — Names that end with AEL or AELLE, which is pronounced ah-el, are typical of Brittany in the north of France.
CLARA — Definitely on the rise in the U.S. as well.


text:
[ONE] RL meeting in Budapest, come it's fun
Dear Citizens of the eWorld,
Today we are writing about a bit different issue. No campaings, no victories, just RL stuff.
A few of you might know that there will be an real life meeting in Budapest at 19-21 August. We have decided to advertise it in as many countries as we can.
International Erepublik meeting - 19-21st August, Budapest
We would like to bring your attention to the first upcoming international meeting that will be held between the 19th and 21st of August in Budapest, Hungary.
Everyone is welcome, we hope that each one of you is going to have a good time here.
So let's see the details:
The date:
- we set the date at the end of August so you can plan ahead. We know that you cannot organise a foreign trip overnight so you have plenty of time to make this long weekend free.
- as some of you know, the 20th of August is a national holiday in Hungary. That means, you are not just given the opportunity to meet players from all over the world but you can also see the the city celebrate and make some awesome photos of the grand firework
- we try to accomodate as many people for free in our homes as possible. Despite of that some of you may have to book a room in a hotel or hostel. We are here to assist you with any questions you might have and we will also try to look up the most fairly priced rooms in town.
The program:
We personally feel responsible about you having a good time once you visited our country.
Sightseeing tours will be provided for free, you can see the plan below and come to as many or as few proposals you like. (The sightseeing tours themselves are going to be free of charge, however if you would like to see an area you should finance entry fees for yourself. We are working on group discounts so you don't have to go bankrupt on Hungarian culture.)
19th of August:
- arriving in town, people meet their hosts/ check in their booked rooms.
- getting ready for clubbing.
- 20:00 CET - ? CET clubbing
20th of August:
- 10: 00 CET Castle District, Buda
- cca. 12:30 CET arriving @ Margit-sziget (Margaret Island)
- cca. 15:00 CET (I personally suggest this appointment since I think we should spend the hottest day of time under some nice tall trees
🙂) Szent István Bazilika (Saint Stephen Basilica)
- cca. 16:30 CET Hősök Tere (Heroes Square)
- cca. 17:30 CET Free time
- in the evening: watching fireworks, clubbing
21th of August:
plan a)
You are early birds and leave the country
You come visit the Ráckeve aquapark with us
All houres are rather estimated than fixed, it depends on you, how much time would you like to spend at a given destination. This schedule is made upon my experience as a tour guide.
If you are interested in participating, keep in touch with at least one of the following people:
srnica/sekica18 - on behalf of Serbia
alpho - on behalf of Romania
Kita Skowronski / AThompson - on behalf of Hungary
Manedhel on behalf of Poland
/more contact persons and their links will be added later on/
We hope that many of you will come to Budapest. Cya there!
Secretary General: Gregory Gallangher
Military Commander: StrozeR
Deputy Secretary General: Mikel_Ahone
Deputy Military Commander: Baltazar8
Department of International Relations : AThompson
In the end I would like to invite every friendly citizen of the ONE, every honorable opponent and troll to our public IRC channel on Rizon: #one.public
National translations
If you have any, please either comment or send a PM to the ONE HQ org we will put it here.
Hail Mexico
Hail Poland
Hail Spain
Hail Macedonia
Hail Serbia
Hail Hungary
Hail ONE
NOTE: I live in Mexico and I need morte that €750, I have the half of this (in Mexican money)
goddamit, i'll be somewhere in France on 19-21 August. But im planning to visit Budapest by the end of April, any erep meetings? : )
Being a casual Bakan guy the first question popping in my mind is "will the security check for granades?".
sad you guys try so hard for a game that blows. this would have been respectable a year ago
This will be realy nice,i cant come i dont have 18 years 😒 next summer i`m coming!!!!!!!!!! 😃
Joe Newton, these meetings were never about the game. Even when it was still good.
I think EDEN will wait that date to win a battle xD coz all ONE 'll be there... ONE for ALL , ALL for ONE 😁
Hail Brotherhood...
from a Turkish citizen.
Serbs are coming since it's only a few hours drive from Belgrade.
ps: I have been in Budapest 2 times great city nice people, but doesn't have night clubs as much as Belgrade
Slovenians might come also.
One question... is there any camps around there? Its summer so we can also camp = )
smooth ziga, i googled camping + budapest (try this out! ) - there are many results ;P
What is this?You are reading an article written by a citizen of eRepublik, an immersive multiplayer strategy game based on real life countries. Create your own character and help your country achieve its glory while establishing yourself as a war hero, renowned publisher or finance guru.


text:
Configuring Your LedBorg as a Rain Indicator
For this portion of the tutorial we'll be combing several things together in order to turn our LedBorg LED module into a rain indicator based on the weather forecast for our location. We'll use a Python script to call a weather API which will in turn read the chance of rain for the day, and then toggle the LED from off to bright blue to indicate the forecasted rain.
First, we need to get an API access key for Weather Underground. For personal use and small development projects the API is free. Visit the Weather API sign up page here and sign up for an API key.
Once you have your API key, visit the Weather Underground and search for the city you want to monitor. In our case we're going to monitor San Fransisco, CA. The URL for San Fransisco's forecast page is:
The important part for our purposes is the last part of the URL: /CA/San_Francisco.html. We're going to use that to edit the forecast URL for the API tool. The base URL is:
http://api.wunderground.com/api/YOUR API KEY/forecast/q/STATE/CITY.json
You can generate the forecast for any US city by entering your API key, the two letter state code, and the city name from the URL you pulled from your Weather Underground search results.
Once you have the API URL with your API key and state/city inserted, you can then edit the following Python script by creating a new text document on your Pi using Leafpad and pasting the following code into it:
from urllib2 import urlopen
import json
req = urlopen('http://api.wunderground.com/api/YOUR API KEY/forecast/q/STATE/CITY.json')
parsed_json = json.load(req)
pop = int(parsed_json['forecast']['txt_forecast']['forecastday'][0]['pop'])
# The following is a debugging value.
# Edit out the hash and change
# the integer to a 0-100 to test
# the LED response.
# pop = 0
print 'Current chance of precipitation is {}.'.format(pop)
# The default setting is to turn on the LED
# for any chance of rain above 20%. You can adjust
# the value in "if pop > 20:" as you wish.
if pop > 20:
LedBorg = open('/dev/ledborg', 'w')
del LedBorg
print ('Rain!')
LedBorg = open('/dev/ledborg', 'w')
del LedBorg
print ('No rain!')
Save the file as wunderground.py in the /home/pi/ directory. Open up the terminal and type in the following command:
python wunderground.py
If you have entered your API key and your state/city codes properly, it should kick back a response that looks like:
If precipitation is predicted for your area your LedBorg output should look like such:
Now waiting for a rainy day to properly test the script would be tedious. If there is no chance of rain in your area today and you want to see the LED light up, edit the wunderground.py script and replace the "pop = pop" passthrough value in line 13 with a value greater than 20 such as the 60 that our forecast returned. Just remember to change the line back to "pop = pop" when you're done.
The final step is to set up a cron job to automatically run the script we just saved on a regular interval in order to keep the LED indicator current. Since this task is necessary for both this script and the email indicator that follows, we're going to cover setting up a cron job after we've shown you how to set up the other script.
Configuring Your LedBorg as a Gmail Indicator
Who doesn't like the dopamine fix that comes with seeing new email in their inbox? In this portion of the tutorial we are going to show you how to use the LedBorg as an new Gmail indicator. Just like last time, we are going to combine an external input (in this case an Atom feed instead of an API) and a simple script to drive our LED.
We need to expand the functionality of our Python installation a little bit by installing FeedParser, a Python RSS/Atom feed reading tool. Open up the terminal and enter the following command:
sudo easy_install feedparser
Once the installation is complete we can set to work creating our Gmail checking script. Again, using Leafpad, paste the following text into the editor. Change the username and password to match the username and password of the Gmail account you wish to check.
import feedparser
# Enter your Gmail username
# and password. Don't include
# the @gmail.com portion of
# your username.
username = "username"
password = "password"
mail = int(feedparser.parse("https://" + username + ":" + password +"@mail.google.com/gmail/feed/atom")["feed"]["fullcount"])
# The following is a debugging value.
# Edit out the hash and change
# the integer to a 0 or 1 to test
# the LED response.
# mail = 0
if mail > 0 :
LedBorg = open('/dev/ledborg', 'w')
del LedBorg
print ('Mail!')
LedBorg = open('/dev/ledborg', 'w')
del LedBorg
print ('No mail!')
Save the script as gmailcheck.py. Open up the terminal and type the following command:
python gmailcheck.py
If you have email sitting in your Gmail inbox the LED will turn green and you will get a response like such:
If you have mail in your Gmail inbox your LedBorg will look like so:
Just like the rain checking script, we have included a debugging value. If you don't have any new email you could either send yourself an email to raise your inbox count to 1 or you could edit out the comment hash and change the debugging line to "mail = 1" to test the script. Remember to revert the line when you're done testing.
Hop into the next section of the tutorial to set your Gmail script to run on a schedule.
Setting Up a Cron Job to Run Your Script
Now that we have two scripts to play with, we need to set up a cron job in order to run them throughout the day to keep the LED indicator current.
The first thing we want to do is to turn off the LED if it is currently on from our previous experiments. At the terminal type:
echo "000" > /dev/ledborg
While you're still at the command line, you can open up the cron editor. If you have never set up a cron job before, we would strongly suggest checking out our guide to using it here. That said, we'll walk you through setting up a basic schedule right here.
At the terminal type:
sudo crontab –e
This will open up the Raspbian cron table in the Nano text editor. Use the arrow keys to scroll down to the very bottom. This is where we're going to setup the recurring cron job for our Python scripts.
If you want to set up the rain script, enter the following line into the cron table:
*/5 * * * * python /home/pi/wunderground.py
Press CTRL+X to exit; select yes to save and overwrite the existing cron table. The value we entered in the cron table "*/5 * * * *" sets the script to run every 5 minutes, forever.
Every 5 minutes is a fine span of time for a script that checks for forecasted rain—you could even argue it's a bit too aggressive—but if you're trying to keep on top of your email it's a bit too long a span for notification. If you're setting up the schedule for the Gmail notification script enter the following line in the cron table:
*/1 * * * * python /home/pi/wunderground.py
This entry runs the gmailcheck.py script every minute for a much speedier update notification.
That's all there is to it! You can experiment with your own Python scripts by lifting the if/else statements out of ours and trying them out with brand new variables. If you can find an input source for the data you can turn it into a variable in your Python script—stock market averages, pollen counts, Twitter mentions, if there is an API for it you can turn it into an ambient LED indicator.
Finally, I want to give thanks to all the great resources I used to work on this project. It has been a bit over a decade since I was actively writing programs and it took a few knocks to get the dust and rust out. The contributors at /r/LearnPython helped me muck about in the API output for Weather Underground, studying how Michael over at Mitch Tech dealt with the Gmail Atom feed made parsing it for the LedBorg easy, and studying the Python learning modules at Code Academy was a great way to pick up the basic syntax and structure of a language I'd never used before.


text:
Hot on the heels of NBC renewing DAYS through September 2014 and Prospect Park officially releasing the cast and production lists for AMC and OLTL comes word from deadline.com that B&B is next to be reupped and GH is in no danger of cancellation. For the full story on the good news in soaps, go to http://www.deadline.com/2013/01/bold-and-the-beautiful-general-hospital-returning-soap-opera-comeback.
More Renewals In Daytime


text:
|Sun, 15th Oct - EAF Judges Workshop (Foxton Village Hall, Hardman Road, Foxton, Cambridge, CB22 6RN)
|Sun, 5th Nov - Dianne Owen & Nadir Khan: EAF Special Event (Fulbourn Center, Home End, Fulbourn, CB21 5BS)
|Sat, 21st Oct - PAGB Masters of Print (On line competition - The closing date for entries is 21st October 2017)
|Sat, 25th Nov - PAGB Awards for Photographic Merit - November 2017 (Colchester Academy, Hawthorne Avenue, Colchester, Essex, CO4 3JL)
|Sat, 30th Sep - RPS Nature Group Annual Exhibition (Wingfield Barns, Church Road, Wingfield, Diss, IP21 5RA)
|Sun, 8th Oct - RPS Creative Group: Lectures by Tony & Eva Worobiec (Foxton Village Hall, Hardman Road, Foxton, Cambridge, CB22 6RN)
|Mon, 2nd Oct - Shillington & DCC: "Specialising in Lighting of all Things" by Colin Bristor (Village Hall, Hexton Road, Barton-le-Clay, Beds, MK45 4JY)
|Mon, 2nd Oct - Chingford PS: "Depth of Feeling" by Terry Ward (The Horticultural Hall, Larkshall Road, Chingford, London E4 6PE)
|Tue, 3rd Oct - Colchester PS: "Creative Photography" by Barbie Lindsay EFIAPs MPAGB FBPE (Christ Church, Ireton Road (off Maldon Road), Colchester, CO3 3AT)
|Sat, 28th Oct - Sat, 25th Nov Loughton CC: Annual Photographic Exhibition (Loughton Library, Traps Hill, Loughton, IG101HD)
|Sat, 28th Oct - Sun, 29th Oct Chet Valley PC: Annual Exhibition (The Community Rooms, Hobart High School, Loddon, NR14 6JU)
<< Start < Prev 1 2 3 4 5 6 7 8 9 10 Next > End >>
|Page 10 of 38
Copyright © 2009-2017 East Anglian Federation of Photographic Societies. All rights reserved.
Powered by Joomla! Styled by JoomlaShine. Bolted together by David Steel. Maintained by Steve Robinson.


text:
Sorry for the late post today. Life just keeps running.
This week I'm trying to sound creative in saying new things I'm blessed with, or saying it in new ways. I wonder what the point of being redundant is. Then I think, is it redundant to tell your spouse "I love you" often? Well, personally I like it.
I don't think God tires of praise. I don't think he gets tired of gratefulness. I think that putting aside complaints and focusing on thankfulness needs to happen at least as often as a garden needs to be weeded.
I feel so spoiled lately. I'm certain this is not because I have everything I want, but because God has given me so much joy.
I wish that didn't sound sappy. I wish I could write it in a way that wouldn't sound fake or like a cheesy card. I know everything in this world that sounds "real" has a certain reality of sin and wrongness and pain in it. Everything else sounds sappy.
Maybe that's why joy is so great. It does not require that everything is perfect. I does not mean the lack of pain, or perfect situations. Joy is not circumstantial. It's not saying everything in my life is perfect and I'm just happy all the time. It's saying my heart and mind are captured by God and so things around me, perfect and imperfect can't grab it.
I feel like I haven't put the time into spending time with God lately as I should. He has been overwhelming me with joy anyway. I've been feeling tired (though I was blessed with a really long nap yesterday! It was lovely!) Still God has given me joy. It has been dropped down on me like an act of mercy and grace, and I wish I could say I have earned it, or did something special so that I could tell you which steps to take to get it.
All I can do is point to the cross, as we've been trying to reflect on during this Lent season. I can just point there and say "I got it there. There's a man there handing it out for free. Not only does he have power and authority to hand it out, he has the grace and love as well." I have no 5 steps to follow, I cannot say I do right all the time. Today I'm just grateful for joy. It sees me through some days when I fail at mustering it up myself.
I'm thankful for the cross. We're so blessed.
You know the deal here. Please leave a comment saying how God has blessed you this last week, and/or put a link to your own "I'm Blessed" post if you're long-winded like me. 😉


text:
Archive for January 15th, 2008
Copycense Clippings (Jan. 8, 2008 to Jan. 14, 2008)
Editor's note: Copycense writers and editors compiled this version of Clippings during our annual holiday break. It was published in mid-January.
Article of the Week
projectb15ck. UCSC Network Woes. Jan. 9, 2008. According to an e-mail within the body of the post, the information technology department at University of California, Santa Cruz is using Cisco's Clean Access appliance and software to "help speed up the network for legitimate uses and reduce the risk of 'accidental' copyright infringement." In the e-mail, John Rocchio , a UCSC IT administrator, says Clean Access will be configured to block file sharing services such as Gnutella and Bittorrent. The poster notes UCSC is a public university, therefore it is spending public funds to block Internet access. The unnamed writer asks incisively what difference is there between UCSC's action's and Comcast's reported filtering of P2P traffic. Categories: File Sharing, P2P & Downloads; Education; Music; Networks; Privacy & Security.
Quote of the Week
"2008 has to be the year we get real or the business as we know it goes away." –Fred Goldring, entertainment attorney.
Elizabeth Montalbano. Digital Music Industry Challenged to Follow Fans' Lead. PCWorld.com. Jan. 9, 2008. You're kidding us, right?
Copycense™: Incisive IP.™
Clippings continue on the next page.
Technorati Tags: Copycense, Copycense Clippings, K. Matthew Dames


text:
Can we hook this week's winner up with Manti Te'o?
Jill ("Dance Moms")
Abby was obnoxious with all of her instigating about Sophia and with forcing a competition between Maddie and Kendall, but Jill was the worst. Why? Because she wouldn't just let Melissa off the hook, and started fighting with Abby in front of the kids. Even when her own daughter started crying uncontrollably and begged her to stop it, she didn't listen.
Watch a recap of this week's "Dance Moms":
Shain ("Buckwild")
We've been chalking these people up as only a danger to themselves, but really, Shain is the worst of the bunch. His attitude towards women is really awful... particularly for a man who still lives at home with his parents. His constant reference to how women should be more like trucks and him being just so charming about the "lucky" gals who got to sleep with him made us want to toss him in the creek. That and his senseless destruction of perfectly good trucks on a regular basis earned him a spot on the list.
JWoww's Dad Terry ("Snooki & JWoww")
Papa JWoww (who looks like an older, sleazier Tom Petty) dropped by his daughter's house for a casual BBQ and proceeded to make things super-creepy by hitting on one of her nubile young friends, even climbing into the hot tub with her wearing swim trunks he borrowed from Roger. (Ol' Rog probably won't want those back anytime soon, especially after the old guy joked about sporting "a different kind of wood.") We agree with JWoww (for once): "It's gross."
[Video: JWoww Belly Flops on Fox's 'Stars in Danger: The High Dive']
Josie ("Top Chef")
Don't let the door hit ya on the way out, Josie. Instead of owning up to her fault in the Kristen debacle last week, she instead was crying because people thought she threw Kristen under the bus. She did! Then she continued to make excuses for her poorly made food, blaming equipment and claiming that other people enjoyed it. Hero of the week? Tom Colicchio, for calling out her out on all her B.S.
Josie talks about her "Top Chef" elimination:
Rod/KJ ("Catfish")
So he's a guy who was lying on a gay dating site about his name and photos and who met a transgendered woman and fell in love, but then he freaked out a bit when he found out that she was actually born a woman and had a child. Not the worst thing, and after they both revealed their deceptions, she totally forgave him and wanted to spend time with the man who had supported her via text/phone during her bout with cancer. But then he told her that the only reason he'd been supportive was because she'd been paying all his phone bills and sending him cash. Douche.
Winner: Rod/KJ
All we know for sure is that he's got some issues he's not ready to deal with and Ebony is way better off with her new lesbian girlfriend who isn't only after her for her money.
More from Television Without Pity:
- Best & Worst Movies: Cast Your Vote
- 'American Horror Story': Craziest Moments
- 'Parenthood': Why It Deserves Another Season


text:
This web site is designed to be your best source for your real estate needs in the Northwest Suburban Chicago land market. The links below are offered freely to help answer your questions concerning residential housing, looking at properties for sale, or trying to understand what your home is worth. I pride myself in offering unparalleled service to ALL my clients in the Chicago land real estate market. Your complete satisfaction with my service and representation is my number one priority.
I am a Realtor Broker offering my services for the past almost 30 years in the entire Chicago real estate market specifically the surrounding communities such as Park Ridge, Des Plaines, Arlington Heights, Mount Prospect (also known as Mt Prospect), Palatine, Hoffman Estates, Schaumburg, Elk Grove Village, Prospect Heights, and Buffalo Grove and Wheeling.These are communities that are considered suburbs on the Northwest side of Chicago. You'll find these areas convenient for hoping on a train, or expressway to Chicago, go to O'Hare airport, or just enjoying the community events in each suburban village or city.
Whether you are considering buying a home, selling a home or both, I know these areas inside and out.
Featured Property for Sale: Some of the best properties for sale are displayed right here. As experts in the Chicagoland real estate market, we can provide you detailed information on these homes, or any others!
Market Trends Newsletter: Find out what's going on in today's real estate market. Our Real Estate Blog provides fresh perspective on our market activity. Be sure to check these out!
Email Listing Alerts: Be the first to know what's coming up for sale in the Chicago land real estate market with our New Property Listing Alerts! Just tell us what you're looking for and we'll email a daily update of all homes listed for sale since your last update. You can unsubscribe at any time.
What's Your Home Worth: Receive a complimentary analysis of your home's approximate present value on the market today.
Avoid Foreclosure: Are you facing foreclosure? You may have a better way out. Contact us today!
Let me know if there is something specific you are looking for and we'll find what you need. Enjoy your visit and please contact me if there is anything I can do to make your next home buying or home selling experience the best it can be!
Your Real Estate Advocate,
Jan McNulty, ABR, CRS, CDPE, Broker/Assoc.
RE/MAX suburban
Office: 847-259-0202
Mobile: 847-274-0535
Office fax 847-259-0175 Email fax: 847-637-8291
330 E. Northwest Highway
Mount Prospect, IL 60056
Office: 847-259-0202
Mobile: 847-274-0535
We can help home sellers understand buyer expectations and establish realistic pricing for your home under the current market conditions.
Our goal is to leverage our knowledge and experience to help you get the best price the market will support while still selling your home in a reasonable period of time.
More Info
For home buyers, our knowledge of the Arlington Heights, Mt Prospect Illinois real estate market is second to none. We know the neighborhoods, the current market conditions, and the opportunities for buyers in today's market. Our buyer specialists are committed to helping you find the home you want at the best possible price.
More Info
Finding your dream home or finding a buyer is only part of the challenge of a successful transaction. There are many details that need to be managed through closing, and Jan McNulty - RE/MAX Suburban brings the depth and breadth of skills and experience required to ensure your transactions go smoothly. Our commitment is to provide you the quality of service and communication you desire and deserve.
Learn More About Jan McNulty - RE/MAX Suburban


text:
Autossh monitors a ssh connection and reconnects the ssh-session if the connection fails.
To automatically log in you need to use an authentication key.
The package, slightly outdated, can be found in the 'oldpackages' feed.
Alternative packages:
sshtunnel is a simpler, functionally-identical package. (depends on OpenSSH so higher disk space requirements)
Install autossh
opkg install autossh
Create a key
dropbearkey -t rsa -f /etc/dropbear/id_rsa
dropbearkey will print the public key, starting with
ssh-rsa. You can reprint the key using
dropbearkey -y -f /etc/dropbear/id_rsaor write it to a file (e.g.
dropbearkey -y -f /etc/dropbear/id_rsa | grep ssh-rsa > /tmp/pubkey
Add the key to the
authorized_keys file on your server.
E.g. Copy pubkey file to the server an do
cat pubkey >> ~/.ssh/authorized_keys
Autossh is configured using the Unified Configuration Interface (
To get ssh working you need to replace
localhost in
2222:localhost:22 of the
ssh variable to the local ip.


text:
After playing six league games in two weeks, Ramona High School's girls soccer team only played one game during the week of Feb. 8-12. The Bulldogs traveled to Valley Center Feb. 12 and played Valley Center to a scoreless tie.
"It was a very evenly fought game," said Ramona coach Tom Charbonneau. "Neither team could put one away."
Ramona goalkeeper Maria Solis had eight saves in the shutout. She was assisted by defenders Gina Lucas, Samantha Schmidt, Erika Stinchcomb and Emily Vandervort. Charbonneau also cited the efforts of senior center-midfielder Bianca Scott.
"Real strong play in the middle," Charbonneau said of Scott. "Her best game of the season."
Charbonneau attributes Ramona's failure to score on Valley Center's defensive play rather than any deficiencies of the Bulldog offense.
"We had probably four or five good opportunities," he said.
Charbonneau noted that Silvia Villegas had three quality shots which were saved by the Jaguars' goalkeeper.
"Our girls had an outstanding game," Charbonneau said.
Ramona's junior varsity and freshman teams played at Del Norte, which is not fielding varsity programs this year, on Feb. 10.
January rains forced two Monday makeup games which led the Bulldogs to play three games in each of the ensuing two weeks.
The tie against Valley Center was the third consecutive Ramona game that ended in a draw, and the tie was also the Bulldogs' fourth in their last five games. Including a non-league forfeit loss that had been on-field tie, Ramona's overall record following the Valley Center game was 3-7-7 while the Bulldogs had a 1-4-5 Valley League mark.
Ramona's final regular-season game will be at home tomorrow against San Marcos and will be Senior Night. The CIF playoff seeding and selection meeting will take place Saturday.
"Still fighting for a CIF spot," Charbonneau said.


text: Only 198 in W.Va. enroll in Affordable Care Act By David Gutman November 13, 2013 Story Share CHARLESTON, W.Va. -- More than 7,000 West Virginians applied for health coverage in the first month of the Affordable Care Act but only 174 of them have completed the process of picking a private insurance plan in the new marketplace, according to data released Wednesday by the federal Department of Health and Human Services.West Virginians completed 3,807 applications, representing 7,096 people, in the period from Oct. 1 to Nov. 2, according to federal officials.Of those 7,096 people, about half -- 3,100 -- were deemed eligible for insurance through Medicaid or the Children's Health Insurance Program.Highmark Blue Cross Blue Shield, the only private insurer offering insurance in West Virginia's marketplace, said that through Tuesday it had enrolled 198 West Virginians -- meaning that an additional 24 people chose a health plan between Nov. 2 and Nov 12."Well, that's not good," Perry Bryant, executive director of West Virginians for Affordable Health Care, said of the enrollment numbers. "It's really disappointing.""But this is a long-distance run, not a sprint," he said. "This is a marathon. Hopefully, the website will be operational sooner, rather than later."Open enrollment in the marketplace continues until March 31.Bryant said he worries that the problems with the health insurance marketplace website, healthcare.gov, will have an adverse effect on rates in the marketplace.The older, sicker people who need insurance will do what it takes to enroll, Bryant said, but website problems could deter younger, healthier people from enrolling in health insurance.Having a sicker population to insure could mean higher premiums, he said. "This has the potential to have a serious long-term impact on the marketplace," Bryant said, "and it's a real concern."Nationwide, about 106,000 people completed the process of selecting a private insurance plan in the first month of open enrollment, far fewer than the 500,000 sign-ups the Obama administration had predicted before the website was beset by technical problems.The 36 states, including West Virginia, where the federal government is operating the health insurance exchange have signed up only 27,000 people, compared to 79,000 for states running their own exchanges.The Obama administration promised Wednesday that the website will be working by the end of November for the "vast majority" of users.That means "an experience for the vast majority of consumers that is easy to use and gets them from start to finish without locking them out or timing them out," Secretary of Health and Human Services Kathleen Sebelius said in a conference call. Sebelius urged shoppers not to wait until the end of the month to shop for insurance, saying that, despite its glitches, the website is "very much operational.""It is running right now," she said. "Every day, people are coming through; every day people are getting enrolled."She said customers who are not eligible for Medicaid or a tax subsidy could browse for plans on the website but then sign up directly with the insurer they choose.Highmark officials stressed the importance of getting the website to work."There is an urgent need to improve the functionality of the federal marketplace as soon as possible, to ensure that consumers have enough time to enroll," Highmark said Wednesday in a news release.Of the people who have applied in West Virginia, 4,371 -- 61 percent -- have been found eligible for some sort of financial assistance, either through Medicaid, CHIP or tax subsidies. That's on top of the 52,000 West Virginians who pre-qualified for the state's expanded Medicaid program, which was enlarged as part of the federal health-care reform package.The deadline to sign up for insurance that would take effect on Jan. 1, 2014, is Dec. 15. People have until the end of March to sign up for insurance for 2014 and avoid the penalty of $95, or 1 percent of taxable income, whichever is greater.Sebelius and the HHS report pointed to enrollment numbers in Massachusetts, which enacted health-care reform in 2006, to try to put the sign-up numbers in a better light.In its first month of open enrollment, Massachusetts had only 123 people sign up and pay for health plans, 0.3 percent of the final total. In contrast, Sebelius pointed to the 106,185 people who signed up in the first month of the Affordable Care Act as representing 1.5 percent of the final goal of 7 million enrollees.One crucial difference is that the Massachusetts numbers included only people who had paid a first-month premium, while the ACA numbers include everyone who has completed an application and selected a plan, even if they have not yet paid.Sebelius said some people might decide to switch plans between now and Dec. 15, when payment is due, so they would not release the number of paid enrollments until then.West Virginia Attorney General Patrick Morrisey, a longtime opponent of the Affordable Care Act, called again Wednesday for delaying the law."Now is the time for people on both sides of the political spectrum to come together and delay this law," Morrisey said in a news release. "Our state and nation can ill afford to keep pursuing something that has proven, on so many fronts, to not work as the president has promised."Staff writer Lori Kersey contributed to this report. Reach David Gutman at firstname.lastname@example.com or 304-348-5119. Story Share Story Share Trending Now 1 Facebook founder visits Cabin Creek Health Systems 2 Volunteers aid flood victims in Elkview, Clendenin 3 Teen pleads guilty to voluntary manslaughter in Oakes Field shooting 4 WVU hopes to get injured defenders back for TCU 5 Huggins, WVU tap South Florida hoops talent Makeshift clinic in typhoon-hit city overrun with patients Previous Story Jones rips Kanawha GOP chairman for levy opposition Next Story


text:
I started walking back to my room at the Chongzuo EcoPark the other evening after watching the langurs come down the mountain to roost.
I'd stayed watching them settle into their cliff face caves a bit longer than usual and by the time I began hiking back to the reserve's headquarters, it was already dark.
I hadn't bothered to pack a flashlight as I knew the path fairly well and preferred to let the moonlight guide me.
Then, I started thinking about all the warning signs plastered along the park's paths warning hikers about cobras.
I flipped open my cell phone for what little light it offered and grabbed the first stick I could find.
After a couple minutes of fumbling around, Jintong, one of the reserve's staff, drove towards me driving an electric cart. He said he'd just run over a snake and proceeded to unfurl a very recently deceased, very large king cobra.
Jintong hit the snake on the path I was about to walk down, about 100 meters from my room.
Then, before I thought to ask for a ride-anywhere!-he drove on. I froze, convinced every branch and twig I saw on the path before me was a snake.
Pan told me afterwards that I needed to be especially careful this time of year. The temperature here on the edge of the tropics was just starting to rise and snakes were beginning to come out in the evenings to lie in open paths warmed by the sun.
My wife would later tell me it snowed again in Boston and that sounded pretty nice to me.


text:
Flared Nostrils + Rhinoplasty Q&A
My Nose Points and Flares when I Smile, How Can I Change This? (Photos)
When I am not smiling I have this cute button nose, but as soon as I smile my nose flares and overly points it looks awful!! I would like to know what... READ MORE
Flared Large Nostrils After Rhinoplasty
I had rhinoplasty 9 weeks ago. A had a small hump shaved down, my nose bone broken so it would colapse down some, and cartiladge shaved down on the... READ MORE
Will Fixing Nose Tip Also Narrow Nostril Flare?
I have a bulbous tip and my nostrils are flared a little more than I would like (especially when I smile). Apart from that, I like my nose. I am... READ MORE
I have a real problem with my nostrils flaring when I'm talking. Is there a procedure to fix this?
Alar Batten Grafts
Hello, I wanted to know what exactly does an Alar Batten graft achieve? Also will it make my alar base wider or increase the flare? Will it make the... READ MORE
If You Flare Your Nostrils Will They Get Bigger?
If you constantly flare your nostrils will they stay that way? READ MORE
Can a Nostril Wedge Excision Be Performed on my Nose?
Hi there, I had an open rhinoplasty about 1 month ago now. Bulbous tip reduced, tip rotated upward and Alar base reduced with a Sill excision. After... READ MORE
What Will Make my Nose Look Better?
My Nose is Awful, Big & Bulbous, Crooked, Hanging Columella, Retracted Ala, over Projected & Not Rotated! Please Guide Me! I DESPISE... READ MORE
Nostil Flaring After Rhinoplasty
Hello I had septorhinoplasty 10 days ago. I can't seem to help but flare my nostrils. I also sometimes wake from sleep wrinkling my nose. I know... READ MORE
Possible to Get a Better Looking Nose but Look Less Attractive?
I have droopy nose tip , a little hump , flaring nostrils and generally unattractive looking nose. But I consider myself good looking from frontal... READ MORE
Does a Paranasal Implant Increase Nasal Flare?
Hello, I was interested in getting a paranasal implant and was wondering whether it would increase by nasal flare? And how/ if it changes the... READ MORE
Had Rhinoplasty 8 Weeks Ago, Nose is Flat and Nostrils Flare, & Looks Shorter, is it Swelling? (photo)
I had my Rhinoplasty almost two months ago, on july 4th it will be 2 months. My nose hasnt changed much at all since 1st month. looks worse then my... READ MORE
Are "Flaring Nostrils" Post Rhinoplasty dangerous?
I am four days post rhinoplasty. I am a very fidgety person and I can't help flaring my nostrils and poking the tip of my nose (i still have a... READ MORE
I Have Flared Nostrils, and my Nose Tip is Bumpy ....could a Nose Job Fix That?
I'm thinking of having a nose job, I want to have a more pointy tip, and less flare nostrils...is it possible? READ MORE
Flared Nostrils After Rhinoplasty- Is It Swelling?
I had Rhinoplasty 3 weeks ago to remove the droopiness, remove the hump, and push back the tip, i find my nostrils are flares out more than they did... READ MORE
Why are my nostrils flared and wide after rhinoplasty? (photo)
I got a primary septorhinoplasty 7 months ago, had dorsal hump removed, deviated septum corrected, my surgeon broke my bones and when i asked he said... READ MORE
Problems That Will Be Faced After Ear Cartilage Graft Rhinoplasty??
I'm going for a Ear cartilage graft rhinoplasty this dec and i would like to know what risk is involved in this surgery? Will my flaring nostril... READ MORE
Unable to Move/Flare Right Nostril After Rhinoplasty: is This Normal?
Ever since I had the surgery, I could not flare my right nostril. 3 months have passed and I am getting worried. is this permanent? did a nerve get... READ MORE


text:
|Cancer Research
|Clinical Cancer Research
|Cancer Epidemiology Biomarkers & Prevention
|Molecular Cancer Therapeutics
|Molecular Cancer Research
|Cell Growth & Differentiation
Laboratory of Molecular Endocrinology [H. H.] and the Department of Surgical Oncology [P. K. H. C., L. L. P. O., K-C. S.], National Cancer Centre, Singapore 169610 and Department of General Surgery, Singapore General Hospital [P. K. H. C., L. L. P. O., K-C. S.], Singapore 169608
Materials and Methods
Materials and Methods
Recent research on IGF-I and IGF-II have shown these to be potent mitogens for human hepatoma cells (7) , and both IGF-I mRNA (8) and IGF-IRS (7 , 8) have been detected in human hepatoma cell lines. In vitro studies on human HCC cell lines HuH-7 and HepG2 have demonstrated that these cells secrete IGF-II and that the inhibition of IGF-II expression in these cells led to a reduction in cell proliferation (8) . IGF-I and IGF-II mRNA have also been detected in vivo in HCC, but the levels compared with matched nontumorous adjacent hepatic tissue were lower than in HCC for IGF-I mRNA (9) and higher in HCC for IGF-II mRNA (9, 10, 11) . Furthermore, IGF-II fetal transcripts (5.6 and 4.5 kb) were found in HCC (9) , whereas normal IGF-II transcripts were regressed (10) . Coexpression of IGF-II protein and Ki-67 antigen in tumor cells were observed suggesting that IGF-II acts as an autocrine or paracrine growth factors in these tumors (10) . Overexpression of IGF-II in transgenic mice has been shown to increase serum IGF-II and HCC (12) . These observations suggest an important role for IGF-II in hepatocarcinogenesis.
Both IGF-I and IGF-II bind with high affinity to specific IGFBPs, which modulate their bioactivity. At least six IGFBPs have been described (reviewed in Refs. 13 , 14 ). Expression of genes encoding the various IGFBPs has been observed in many tissues and is subject to intricate physiological regulation (reviewed in Refs. 13 , 14 ). IGFBP-3 is the most abundant IGFBP in the circulation, where it forms a Mr 150,000 complex with an acid-labile subunit and IGF-I or IGF-II (14) . The IGFBP-3 gene is expressed in many tissues, and IGFBP-3 has affinities for IGFs that are either equal to or stronger than those of the IGF receptors and, therefore, inhibit the IGFs by sequestration in the extracellular compartment (reviewed in Refs. 13 , 14 ). Recent evidence also demonstrates that IGFBP-3 has growth-inhibitory activity that is independent of its IGF binding properties (reviewed in Refs. 14 ).
Thus, existing evidence suggests that a better understanding of IGFBP-3 may allow modulation of its activities and the potential for therapeutic applications in the control of HCC.
Materials and Methods
To determine whether the proliferative action of IGF-I can be attenuated in the presence of IGFBP-3, HepG2 cells were treated with hrIGFBP-3 in the presence and absence of IGF-I for 48 h. As shown in Fig. 5C , treatment of HepG2 with 25 ng/ml IGF-I for 48 h resulted in a 2.8-fold increase in cell number (P < 0.01). IGF-induced HepG2 proliferation was significantly attenuated (P < 0.01) in the presence of 250 ng/ml IGFBP-3 and completely abolished at the concentration of 500 ng/ml. This result suggests that IGFBP-3 attenuated IGF-I-induced HepG2 proliferation by reducing IGF-I bioavailability.
To test the hypothesis that autocrine production of IGF-II by liver cancer cells plays a role in mediating liver cancer cell growth, HepG2 cells, which has been shown to secrete IGF-II (8) , were treated with anti-IGF-II, IGF-II, or with both combined for 48 h. Fig. 5D shows a 3-fold induction of basal proliferation by 25 ng/ml IGF-II (P < 0.01). This induction was significantly attenuated by IGF-II antibody (P < 0.01), whereas preimmune serum was not significantly affected. IGF-II antibody alone caused a 35% reduction in cell number. These results suggest that the rapid proliferation of liver cancer cells in vivo and in vitro may at least, in part, be a consequence of autocrine stimulation mediated by IGF-II expression.
Because MAPK and PI3k are important for the effect of IGFs on growth and apoptosis, the mechanisms involved in the attenuation of IGF-I by IGFBP-3 on proliferation of human HepG2 cancer cells were investigated. HepG2 cells were preincubated with 250 ng/ml hrIGFBP-3 for 24 h. The medium was removed, and cells were rinsed with serum-free medium and exposed to IGF-I for 10 min. Various components of IGF-IR signaling (IGF-IR, IRS-1, Akt, MAPK, and PI3k) were analyzed. Treatment with IGF-I resulted in a significant increase in tyrosine phosphorylation of IGF-IR ß subunit, IRS-1, and MAPK (P < 0.01; Fig. 6, C, E, and G ). Activation of MAPK by IGF-I also resulted in the activation of Elk-1 transcription factor (Fig. 6, H) . Preincubation of HepG2 cells with hrIGFBP-3 lead to a significant reduction in IGF-I-induced phosphorylation of IGF-IR, IRS-1, Elk-1, and MAPK (P < 0.01). Treating HepG2 cells with IGF-I also resulted in an increase in the association of p85 with IRS-1 (data not shown). A 2.5-fold increase in basal PI3k activity by IGF-I was observed (P < 0.01; Fig. 7A ). IGFBP-3 alone also significantly reduced PI3k activity (P < 0.01). Phosphorylation of Akt at Ser473 was also induced by IGF-I (P < 0.01; Fig. 7C ). IGF-induced PI3k activity and Akt-1 phosphorylation were significantly attenuated by preincubating HepG2 cells with IGFBP-3 (P < 0.01; Fig. 7C ).
Materials and Methods
All of the HCC samples significantly expressed less IGF-I than control liver. Unlike IGF-I, most of HCC samples retained IGF-II expression, and 32% of HCC samples significantly overexpressed IGF-II. IGF-IR levels were not significantly low in 39% of HCC samples. These observations suggest an autocrine/paracrine role of IGFs and IGFBP-3 in the proliferation of liver cancer cells in vivo. This suggestion was reinforced by in vitro experiments involving the IGFBP-3 and IGF antibodies. Blocking IGF-II autocrine function by treating HepG2 cells with IGF-II antibody significantly reduced cell-proliferation indicating that functional autocrine IGF-II loop exists in these cells. Treatment of HepG2 liver cancer cells with IGFBP-3 also led to growth inhibition, whereas treatment of HepG2 with IGF-I or IGF-II led to increased DNA synthesis. The growth-promoting effect of IGF-I and IGF-I-induced phosphorylation of key proteins in PI3k and MAPK pathways were significantly attenuated when HepG2 cells were pretreated with hrIGFBP-3. Our results provide evidence for a paracrine/endocrine mechanism(s) by which IGFs and IGFBP-3 influence hepatoma cell proliferation.
Because endogenous IGF-I and IGF-II are produced by both normal liver cells and HCC cells, the decrease or loss of IGFBP-3 production by HCC would allow more free IGFs to act in an autocrine or paracrine fashion to enhance tumor cell growth. Although expressions of other IGF-binding proteins in HCC such as IGFBP-1 and IGFBP-2 (data not shown) were also investigated, only IGFBP-3 levels were associated with HCC growth suggesting the possible involvement of IGFBP-3 in controlling HCC cell proliferation.
The observations that HCC tumors express less IGF-I than control liver, whereas IGF-II expression is higher in a high proportion of HCC tumors, are consistent with previous reports that showed IGF-I mRNA levels were lower in HCC as compared with nontumorous hepatic tissue from an adjacent area (9) , whereas IGF-II mRNA was higher tumor compared with normal liver (13 , 14 , 16) . At the moment, the molecular mechanisms responsible for reduction in IGF-I and reactivation of IGF-II in HCC remain to be determined. It has been reported that hepatic IGF-I and IGF-IR expression is regulated by GH (13 , 14) and estrogen (16) . It is possible that the reduction in IGF-I and IGF-IR expression in HCC observed in the present study may be because of the loss of receptors for GH or estrogens. Experiments are under way to investigate this possibility.
Currently, the molecular mechanisms by which the IGFBP-3 expression is lost or reduced in HCC are not known. Several reports have suggested that IGFBP-3 expression is regulated by GH (reviewed in Refs. 13 , 14 ), antiestrogen (17) , transforming growth factor ß (18) , and retinoic acid (19) . In HCC, receptors of these compounds may be lost or inactivated, leading to IGFBP-3 gene inactivation. It is possible that the absence of IGFBP-3 expression in HCC is a consequence of genetic alterations such as deletion, mutation, or inappropriate hypermethylation as described in a number of tumor suppressor genes (20 , 21) and cyclin-dependent kinase inhibitor genes (22 , 23) , or chronic hepatitis and cirrhosis (24 , 25) . We are currently investigating the mechanisms responsible for silencing the IGFBP-3 gene and the relationship between hepatitis and cirrhosis with the loss of IGFBP-3 expression in HCC.
In our experimental system, we also observed that IGFBP-3 inhibited proliferation of HepG2 cells and could be an important factor in a negative control system regulating human cancer cell growth in vivo. Because the gene encoding IGF-II is expressed in HepG2 (8) , the observed inhibitory action of IGFBP-3 likely involves the reduction of bioavailability of endogenous IGF-II for cell surface receptor binding. It is also possible that IGFBP-3 inhibits HepG2 by direct growth inhibitory signal transduction pathways (26 , 27) . However, these mechanisms are not mutually exclusive and both may be relevant in vivo.
Human liver cancer cell growth is inhibited by exogenous IGFBP-3. In addition, IGFBP-3 is a potent IGF-I antagonist. These properties suggest a possible therapeutic application for IGFBP-3 in the control of liver cancer cell proliferation. Also, the presence of IGFBP-3 in HCC specimens may possibly be a better marker for tumor stage, diagnosis, and prognosis than currently used histological parameters. Experiments are under way to analyze the association between levels of serum IGFBP-3 and tumor stage, metastasis, and other markers such as serum -feto protein in patients with HCC.
Although many treatment modalities have been attempted in HCC, there is currently no proven practical therapy besides surgery, and attempts at both adjuvant therapy and therapy for inoperable HCC remains experimental (6) . Because HCC is capable of endogenous production of both IGF-I and IGF-II (8, 9, 10, 11) , and hepatoma cells are very responsive to stimulation by these growth factors, agents that are capable of modulating endocrine, paracrine, and autocrine production of the IGFs and their receptors are likely to be useful in treatment of HCC. The present findings offer both a potential diagnostic parameter and as well as a potential novel strategy for HCC cancer endocrine therapy.
|Materials and Methods
Materials and Methods
Collection of Human HCC and Adjacent Nontumor Liver Specimens.
Tissue samples were obtained intraoperatively from tumors and adjacent nontumor livers during liver resection for HCC in 28 patients at the Singapore General Hospital. The samples were snap frozen in liquid nitrogen and stored at -80°C until analysis. A similar set of samples was fixed in 10% formalin and paraffin embedded. The diagnosis of HCC was confirmed histologically in all of the cases. Prior written informed consent was obtained from all of the patients, and the study received ethics board approval at both institutions.
Immunolocalization of IGFBP-3.
Formalin- and paraffin-embedded sections were used for IGFBP-3, IGF-II, and IGF-I immunolocalization. This was performed using rabbit antihuman IGFBP-3 (1:500 dilution), rabbit antihuman IGF-I (3 µg/ml), and rabbit antihuman IGF-II (3 µg/ml) antibodies as described (28) . Nonspecific staining was evaluated for each specimen using either a similar concentration of IgG or by absorbing the primary with appropriate specific immunogen. The slides were evaluated, and intensity of the staining was scored. Specific staining was semiquantitated by assigning a score of 0 to +++ based on increasing green fluorescence intensity. The results shown in Table 1 represent the average score obtained from twice staining.
Cell Culture.
Human hepatoma HepG2 cells were obtained from American Type Culture Collection and maintained as monolayer cultures in MEM supplemented with 10% FCS growth medium. For proliferation study, confluent cultures of HepG2 cells were trypsinized and plated at 2 x 104 cells in 24-well plates (Nunc, Nalgene Nunc International, Rochester, NY) with growth medium. After 48 h, the cell monolayers were rinsed twice with MEM PSF medium and incubated additionally in PSF medium for 24 h. After 24 h, various concentrations of hrIGFBP-3, human recombinant IGF-I, or 25 ng/ml human recombinant IGF-I in conjunction with various concentrations of hrIGFBP-3 were added in triplicate in specific pathogen-free medium for 48 h. Cell number was determined as described previously (28) .
To determine the effect of exogenous IGF-II and autocrine IGF-II production on HepG2 cell proliferation, HepG2 cells were plated and grown as described above, and the cells subsequently treated with rabbit preimmune serum (1:800 dilution), 25 ng/ml human recombinant IGF-II, rabbit antihuman IGF-II antibody (2 µg/ml), or with combination for 48 h. Cell number was determined as described above.
Western Blotting.
To determine changes in the expression of IGFBP-3 and IGF-IR, snap-frozen HCC tumors and normal adjacent liver tissues were thawed and homogenized in lysis buffer (1 mM CaCl2, 1 mM MgCl2, 1% NP40, 1 µg/ml leupeptin, 1 µg/ml aprotinin, 1 µM phenylmethylsulfonyl fluoride, and 100 µM NaVO4). Proteins were subjected to Western blot analysis as described (28) . Blots were incubated with 1:5000 antihuman IGFBP-3, 1 µg/ml antihuman IGF-IR, 0.5 µg/ml anti--tubulin antibodies, and 1:7500 horseradish peroxidase-conjugated donkey antimouse or antirabbit secondary antibody. The blots were then visualized with a chemiluminescent detection system as described by the manufacturer.
To examine the effects of IGF-I and IGFBP-3 on IGF-IR, IRS-1, Elk-1, Akt-1, MAPK, and their phosphorylation forms, HepG2 cells were plated at 5 x 106 cells per Petri dishes (Becton Dickinson, Lincoln Park, NJ) in growth medium. After 48 h, the cell monolayers were rinsed twice with MEM SFM and additionally incubated in PSF medium for 24 h. After 24 h, cells were treated with 250 ng/ml hrIGFBP-3 in SFM for 24 h. Cells were rinsed once and then incubated with 25 ng/ml IGF-I for 10 min. After IGF-I stimulation, cells were harvested and lysed in lysis buffer as described above. To determine the effects of IGFBP-3 on IGF-I-induced MAPK, Elk-1, and Akt phosphorylation, Western blotting was performed using phospho Akt (Ser473; 1 µg/ml), phospho p44/42 MAPK (Thr202/Tyr204; 1 µg/ml) and phospho Elk-1 (Ser383; 1 µg/ml) antibodies. Levels of IGF-IR ß subunit and IRS-1, and their phosphorylated forms were determined by immunoprecipitation of total cell lysates using anti-IGF-IR (2 µg/ml) and IRS-1 (4 µg/ml) antibodies, respectively, as described by the supplier. Immunoprecipitated proteins was blotted using rabbit antihuman IGF-IR (1 µg/ml), antihuman IRS-1 (1 µg/ml), and antiphosphotyrosine 4G10 antibodies (1 µg/ml).
To study IRS/PI3k p85 interactions, cell lysates were precipitated with anti-IRS-1 antibody. Immunocomplexes were used to determined PI3k activity associated with IRS-1. To measure PI3k activity, 500 µg of total protein were incubated with 4 µg of anti-IRS-1 antibody for 3 h. The antigen/antibody complex was precipitated with 50 µl of protein A-agarose and washed with kinase buffer [25 mM Tris (pH 7.5), 5 mM ß-glycerol phosphate, 2 mM DTT and 0.1 mM Na3V04]. Immunoprecipitates were resuspended in 25 µl of kinase buffer containing 20 µg of L-phosphatidyl inositol-4 monophosphate and 5 µl of 100 mM MgCl2, and then adding 30 µCi of [32P]ATP in 2.5 µl of 0.88 mM ATP per kinase reaction. Kinase products were resolved using TLC with a CHCL3/methanol/H2O/NH4OH (40:48:10:5) solvent system. Quantitative analysis of 32P-labeled inositol 1,3,45 phosphate levels was determined by scanning of the blots densitometrically.
Statistical Analysis.
For quantitation analysis, the sum of the density of bands corresponding to protein blotting with the antibody under study was calculated and normalized the amount of -tubulin. Differences in cell number and the levels of proteins under studied were analyzed by the Mann-Whitney U test.
1 Supported by grants from National Medical Research Council of Singapore (NMRC/0541/2001) and SingHealth Cluster Research Fund (EX 008/2001; to H. H.).
2 To whom requests for reprints should be addressed, at Molecular Endocrinology Laboratory, National Cancer Centre of Singapore, 11, Hospital Drive, Singapore 169610. Phone: (65) 436-8347; Fax: (65) 226-5694; E-mail: cmrhth{at}nccs.com.sg.
3 The abbreviations used are: HCC, hepatocellular carcinoma; IGF, insulin-like growth factor; IGF-IR, IGF-I receptor; IGFBP, insulin-like growth factor-binding protein; hrIGFBP-3, human recombinant IGFBP-3; PI3k, phosphatidylinositol 3'-kinase; MAPK, mitogen-activated protein kinase; IRS-1, insulin receptor substrate 1; Akt, serine/threonine protein kinase; GH, growth hormone; PSF, phenol-red serum-free; SFM, serum-free medium.
Received for publication 11/ 1/01. Revision received 12/27/01. Accepted for publication 1/ 7/02.
Materials and Methods
This article has been cited by other articles:
H. Huynh, V. C. Ngo, J. Fargnoli, M. Ayers, K. C. Soo, H. N. Koong, C. H. Thng, H. S. Ong, A. Chung, P. Chow, et al.
Brivanib Alaninate, a Dual Inhibitor of Vascular Endothelial Growth Factor Receptor and Fibroblast Growth Factor Receptor Tyrosine Kinases, Induces Growth Inhibition in Mouse Models of Human Hepatocellular Carcinoma
Clin. Cancer Res., October 1, 2008; 14(19): 6146 - 6153.
[Abstract] [Full Text] [PDF]
H. Huynh, P. K.H. Chow, and K.-C. Soo
AZD6244 and doxorubicin induce growth suppression and apoptosis in mouse models of hepatocellular carcinoma
Mol. Cancer Ther., September 1, 2007; 6(9): 2468 - 2476.
[Abstract] [Full Text] [PDF]
H. Huynh, K. C. Soo, P. K.H. Chow, and E. Tran
Targeted inhibition of the extracellular signal-regulated kinase kinase pathway with AZD6244 (ARRY-142886) in the treatment of hepatocellular carcinoma
Mol. Cancer Ther., January 1, 2007; 6(1): 138 - 146.
[Abstract] [Full Text] [PDF]
H. Huynh, K. C. Soo, P. K.H. Chow, L. Panasci, and E. Tran
Xenografts of Human Hepatocellular Carcinoma: A Useful Model for Testing Drugs.
Clin. Cancer Res., July 15, 2006; 12(14): 4306 - 4314.
[Abstract] [Full Text] [PDF]
C. W. Fong, M.-S. Chua, A. B. McKie, S. H. M. Ling, V. Mason, R. Li, P. Yusoff, T. L. Lo, H. Y. Leung, S. K.S. So, et al.
Sprouty 2, an Inhibitor of Mitogen-Activated Protein Kinase Signaling, Is Down-Regulated in Hepatocellular Carcinoma
Cancer Res., February 15, 2006; 66(4): 2048 - 2058.
[Abstract] [Full Text] [PDF]
M. Takaoka, H. Harada, C. D. Andl, K. Oyama, Y. Naomoto, K. L. Dempsey, A. J. Klein-Szanto, W. S. El-Deiry, A. Grimberg, and H. Nakagawa
Epidermal Growth Factor Receptor Regulates Aberrant Expression of Insulin-Like Growth Factor-Binding Protein 3
Cancer Res., November 1, 2004; 64(21): 7711 - 7723.
[Abstract] [Full Text] [PDF]
H Reynaert, K Rombouts, A Vandermonde, D Urbain, U Kumar, P Bioulac-Sage, M Pinzani, J Rosenbaum, and A Geerts
Expression of somatostatin receptors in normal and cirrhotic human liver and in hepatocellular carcinoma
Gut, August 1, 2004; 53(8): 1180 - 1189.
[Abstract] [Full Text] [PDF]
C. K. Ong, C. Y. Ng, C. Leong, C. P. Ng, K. T. Foo, P. H. Tan, and H. Huynh
Genomic Structure of Human OKL38 Gene and Its Differential Expression in Kidney Carcinogenesis
J. Biol. Chem., January 2, 2004; 279(1): 743 - 754.
[Abstract] [Full Text] [PDF]
L. M. Neri, P. Borgatti, P. L. Tazzari, R. Bortul, A. Cappellini, G. Tabellini, A. Bellacosa, S. Capitani, and A. M. Martelli
The Phosphoinositide 3-Kinase/AKT1 Pathway Involvement in Drug and All-Trans-Retinoic Acid Resistance of Leukemia Cells
Mol. Cancer Res., January 1, 2003; 1(3): 234 - 246.
[Abstract] [Full Text]
|Cancer Research
|Clinical Cancer Research
|Cancer Epidemiology Biomarkers & Prevention
|Molecular Cancer Therapeutics
|Molecular Cancer Research
|Cell Growth & Differentiation